LOCUS       DAOSJM010000001        24321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_2302_length_24321_cov_4.610195, whole genome shotgun sequence.
ACCESSION   DAOSJM010000001 DAOSJM010000000
VERSION     DAOSJM010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 24321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24321
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_24321_cov_4.610195"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PKX82_00005"
     CDS             complement(<1..916)
                     /locus_tag="PKX82_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOF50653.1"
                     /translation="MSVALYSLWTSRETSQTLERLTKQSMVHVVMVAEVQERVVAVNG
                     LVMQTVAYTGAGLKPAIIKALDEKIAAELKTTQQRIDRLREAMAQGDADAVSRLTRLD
                     AAFKTYTKSALDTVDMKDADLATAAIYMSAADTAYQETRGLLSEQFNGEVEQAKHSGE
                     LTTESLSTGNKASLTLAAIALVIGMLVTWFTAREIVRPLKSAVGIARDVAEGNLSLRD
                     VQAGSDETGQVLRALGEVTQRLNGMIANIRSGADQIDTAASEISIGNNDLATRTEQTA
                     SALQATSSSVADLSNTIRLNADTAMQATQ"
     gene            complement(1076..1438)
                     /locus_tag="PKX82_00010"
     CDS             complement(1076..1438)
                     /locus_tag="PKX82_00010"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOF50654.1"
                     /translation="MVNAALAHVKKAGNDQAFKDFTADKANWTKKDLYVFAMDMKGVV
                     LAHGANEKLVGKNLIELKDQNGKGFAREFIEVGSTKGEGWVDYDWAHPVTKKVEGKTS
                     YVKRIPGFDGFVVVGAYR"
     gene            complement(1695..2543)
                     /locus_tag="PKX82_00015"
     CDS             complement(1695..2543)
                     /locus_tag="PKX82_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase family protein"
                     /protein_id="HOF50655.1"
                     /translation="MAQLIGGLGTSHIPAIGNAIHKGLQQNPYWKPFFDGFPPVREWL
                     GEKKPDVVVMFYNDHGLNFFLDKMPTFAVGAAHEYNNADEGWGIPTLPPFKGEVDLSW
                     HLINTLVEKEFDVVTCQEMLVDHACTLPLKLFWPEGDCPVTVVPVCINTVQFPLPSAK
                     RCYALGKAVGEAIRSWDSSKKVAVIASGGLSHQLDGERAGFINKAFDLQFLDSLDTNP
                     EWATQFSVHELVEKTGTQGVELLMWLAMRGALATGGGSSVRRVTSNYHIPISNTATAV
                     LALETA"
     gene            complement(2571..2918)
                     /locus_tag="PKX82_00020"
     CDS             complement(2571..2918)
                     /locus_tag="PKX82_00020"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HOF50656.1"
                     /translation="MSAPLPKIPGTTPFDGEQAKKGYALNKMCFSFNDAANRAAFLAD
                     EEGYMKKYGLTEPQAAAIRAKNVLQLISAGGNAYYLAKFAGIFKLDMQDIGAQQTGMT
                     KDEFKAKLVAAGK"
     gene            3005..3943
                     /locus_tag="PKX82_00025"
     CDS             3005..3943
                     /locus_tag="PKX82_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF50657.1"
                     /translation="MHCMSKFDWSDLDAGLLQLLVAVVEAGSVTGAAQRLGVTQSAVS
                     HLLDKLRGITGDPLFVKSGRGIVATARAESLAEQARELLRQLQQFAHSAEFDPARWKT
                     SITIAANDFQRDLLLPALAQRLRQAAPLVQLRVIPSAVPRLETLRADECQLVISPRPP
                     DGTDILQKRLFETAYRVFFDASQRAAPKTQEEYQAADHATVAYEAHRGLDIDRQFEAQ
                     GLVRRFAVVVPGFSALPAFVRGTPLLVTAPALLANSTFAGLAHAPVPVACPAMPMYLI
                     WHARYQQDPAHRWLREQLEAVVAPALSSPKGSGTIR"
     gene            3943..4752
                     /gene="ppk2"
                     /locus_tag="PKX82_00030"
     CDS             3943..4752
                     /gene="ppk2"
                     /locus_tag="PKX82_00030"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOF50658.1"
                     /translation="MKKSKSGKEPKPGKLDKGAYYEKLAALQLELNEVARWLQHTGKR
                     LVVVIEGRDTAGKGGVISAMAETLNPRQCRTVALAKPSDQEKTQWYFQRYVPHLPGAG
                     EIVLFDRSWYNRAGVERVMGFCSKEEVKAFLKQAPVFERMLVDDGVLLFKYWLTVDQA
                     QQEERFAERAADPLKHWKLSPIDVKAREKYAEYGLARDAMLEATHKPRTPWTLVDFND
                     QRLGRLTLIRHLLDHIPDRHVPESVVTFPPLDHAPLREKFGASPKPIAPAA"
     gene            complement(4765..4914)
                     /locus_tag="PKX82_00035"
     CDS             complement(4765..4914)
                     /locus_tag="PKX82_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HOF50659.1"
                     /translation="MLETVAILLLIFWALGLVSSYTLGGFIHILLVIAVVVILVRLIR
                     GRSPL"
     gene            complement(5113..6630)
                     /locus_tag="PKX82_00040"
     CDS             complement(5113..6630)
                     /locus_tag="PKX82_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HOF50660.1"
                     /translation="MSNQTRVRHPGYLSKIMSAEAAAALIPAGANVGMSGFTGAGYPK
                     QVPEALARRIERLHAAGEDFRIGLWTGASTAPELDGALAAVNGISMRLPYQSDPTVRK
                     QINAGATQYTDIHLSHVAQLAWFGFLGPLNVAVVEVAGILPDGRLIPSSSVGNNKTWL
                     DHADHIILEVNSHQNIGLEGMHDIYYGTRLPPLRRPIPLLHPGDRIGDHYLQCDLSKV
                     IAVVETQQPDRNSAFAAPDENSERIAGHIIDFLQREVKAGRLPPELLPLQSGVGNIAN
                     AVLIGLNKGPFENMTAYTEVLQDGMLDMLRSGKLAMASATALSFSPAALADFNANIDF
                     YRERIVLRPQEISNHPELIRRLGLIAMNAMIEADIYGNVNSTHVMGSSIMNGIGGSGD
                     FARNAYLSFFMTPSTAKNGAISCIVPMVSHTDHTEHDVEIIVTEQGLADLRGLSPTQR
                     ARLVIEKCAHPDYQPALRDYFERALRDAPGKHTPHLLTEALSWHARCVETGRMLA"
     gene            6775..7584
                     /locus_tag="PKX82_00045"
     CDS             6775..7584
                     /locus_tag="PKX82_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOF50661.1"
                     /translation="MNSEAIWTFLTTQGADFGLKLLGALAAWIIGRWLIAMALRLLNA
                     AFQRGGKVDQTLANYLTSILSVILNIVLILAILDIFGVKTTSFAALLAGAGLAIGTAW
                     GGLLTHFAAGVFMQVLRPYKVGDFVTAGGITGTVKELGLFGTTLVTPDNVVTIVGNNT
                     VFSGAIQNFSTLPHRRVDCLAKVANSVDVVDAIARLKVAVAAIPNVATSPAPDIEILQ
                     FTPEGPLLCVRPYTYTDHYWQVYFDTHKAVVSTFGTAGYPVPETPVVHRNL"
     gene            complement(7657..9003)
                     /gene="miaB"
                     /locus_tag="PKX82_00050"
     CDS             complement(7657..9003)
                     /gene="miaB"
                     /locus_tag="PKX82_00050"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOF50662.1"
                     /translation="MSKKVFIKTFGCQMNEYDSDKMADVLGAAQGYEPTDDVEQADLI
                     LFNTCSVREKAQEKVFSDLGRIKHLKAKGVQIGVGGCVASQEGAEIIKRAPYVDVVFG
                     PQTLHRLPEMLNARKAQNRPQVDISFPEIEKFDHLPPAKVEGASAFVSIMEGCSKYCS
                     YCVVPYTRGTEINRPFEDVLVEIAGLADQGVKEVNLLGQNVNAWRAPMTEGGEIADFA
                     TLLEYVAEIPGIERIRYTTSHPNEFTPSLIAAYDKLPKLVSHLHLPVQHGSDKILMAM
                     KRGYTAMEYKSTIRKLRAIRPDMSISSDFIVGFPGETDDDFARMMKLIADIGFDNSFS
                     FIFSPRPGTPAANLHDDTPHEVKLARLQELQAAINANITRISEQRLGTVQRILVEGAS
                     KRDNGELMGRTECNRVVNFAGDARLVGQLVDVKITETRSYTLRAEVLTRETAALPL"
     gene            complement(9094..9495)
                     /locus_tag="PKX82_00055"
     CDS             complement(9094..9495)
                     /locus_tag="PKX82_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50663.1"
                     /translation="MNRCLRALNLLCLLTALPLAASAQTSPTTQVLTDTTGTVSVAVR
                     TFPPKAVRGTLMVVAPPEVVLNNRIDRLSPGARIRGANGMLAMSASLVGQTLPVMTLR
                     ESQGMLHEVWVLTDIEAKLYPATPSYSSAAQ"
     gene            complement(9538..10542)
                     /locus_tag="PKX82_00060"
     CDS             complement(9538..10542)
                     /locus_tag="PKX82_00060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOF50664.1"
                     /translation="MQQRSLGPFQVSAIGLGCMNLSHAYGAPVSAEQGERVLLEALDA
                     GVTLFDTATLYGFGANETLVGKVMQPHRNRFTLASKCGMGGVDVAGDGKLVRVIDGRP
                     ETIRQQCEDSLRRLRTDVIDLYYLHRWDKKVPIEDSVGALADLVRAGKIRSIGLSEVS
                     ADTLRRAHAVHPITALQTEYSLWTRNPEIAVLDACKELGIAFVAFSPVGRGFLCGDLA
                     DVDTLDAKDIRRSMPRFAPDNYAANLALLPAYRALAQEMDCTPAQLAIAWLLHKAPHI
                     IPIPGTTRVDHLHDDLGAADVQLSADAIARLEALIHSGNVVGSRYNPQGNSEVDTEVF
                     "
     gene            complement(10624..10700)
                     /locus_tag="PKX82_00065"
     tRNA            complement(10624..10700)
                     /locus_tag="PKX82_00065"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10664..10666),aa:Met,seq:cat)
     gene            complement(10736..11515)
                     /locus_tag="PKX82_00070"
     CDS             complement(10736..11515)
                     /locus_tag="PKX82_00070"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOF50665.1"
                     /translation="MNYECITTRIEGDKVAIITLNRPKQLNALNDQLMDELGHALKGF
                     DLNPAIGCMVITGSEKAFAAGADIGAMATYTFADVYKGDYITRNWEVIRSIRKPIVAA
                     VSGFALGGGCELAMMCDFIIAADNARFGQPEIKLGVIPGAGGTQRLPRAIGKSKAMDL
                     ALTGRMMDATEAERSGLVSRVVPLDKLQEEALGAALLICGFSQMATMAAKEAVNRSFE
                     GTLADGIMFERRMFHALFATADQKEGMDAFVNKRPANFKHQ"
     gene            11575..12369
                     /locus_tag="PKX82_00075"
     CDS             11575..12369
                     /locus_tag="PKX82_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOF50666.1"
                     /translation="MADPTVLFADHGNVALVTLNRPDALNSLTHQMHRDLWAALDRAE
                     ANPAIRALVLTGAGRGFCAGADLHGSDLAPSDGAAGRPDPGVTIEQNFNPTTRRMQNL
                     RMPCVAAVNGVAAGAGVSLALACDVTIAAPGARFIQAFSKIGLIPDAGSSWFMPQRLG
                     LARAMALAMTGDALSAPQAKEWGLIWDVADDCVAAALAYAQRLADMPTLALVATRHLL
                     RESTARTLNQQLDVERDTQSAMGKTHDYVEGVTAFREKRSARFNGT"
     gene            complement(12366..14204)
                     /locus_tag="PKX82_00080"
     CDS             complement(12366..14204)
                     /locus_tag="PKX82_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HOF50667.1"
                     /translation="MTRSPAGRAGSPADIHYRVEMVNLHAHLYQVTLTIAQPAAQQHV
                     QLPAWIPGSYLVREFAKNLQNLRANQSGRAVELQQLTKARWQVDCTAHAPLELQYEVY
                     AFDNSVRTAWLDATRGFFNGTSLFLMVEGQQDKTHTVDLVACTDSPQWKVATGLPPVR
                     TDKRGFGRYSASNYDMLADCPVELGDFWEGHFQACGIPHRFVVAGAPPSFDGTQLLKD
                     TQTICEEGIRFWHGKNYKNNSASRTTGGGKGPIPFQNYLFILNATHDGYGGLEHRNST
                     VLLCTRKDLPRQPRTTLAPTTHKQPEGYTTLMGLICHEYFHTWNVKRLRPAEFSRYDY
                     TQENYTELLWFFEGFTSYYDDLLLRRAKLIDNTVYLKLLSKTINQVQQTPGRLVQTVA
                     QASFDAWVKYYRQDENTPNATVSYYTKGSLVALCLDLTLRAEGKTTLDEVMRGLWKRC
                     AGGPMAQDDLLAVLSELGGRAYAKDLARWVHSTKDLPLTELLEAHGLMVNREPDQIAQ
                     QLGLRVKESGGLFVQQVLRASVAEKAGFAAGDEWIAVEAESGPWRMQTLDDLNIYTGK
                     AKKVTALVARDKRLMTLSLTMPKPSQAVRLSVRDAAAANKWLDGGA"
     gene            complement(14201..14926)
                     /locus_tag="PKX82_00085"
     CDS             complement(14201..14926)
                     /locus_tag="PKX82_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOF50668.1"
                     /translation="MISRFFPALLGTTLAMAALGAGAQEAVIRKNLAERVPQLKAIDE
                     VTKSVIPGLYEIRVNGTEIYYSDAQANYLVQGNIIDVRNRRNLTEERVDKLTAIKFDA
                     LPLKDAFTIVRGNGKRKLAVFEDPNCGYCKRFERDLQKVDNVTIHMFLYPILGADSAE
                     KSKAIWCAKDKTAAWQDWMVRDQAPPSAAAMCDTSALARNVELGRAYKITGTPTLVFV
                     NGSRVPGAIDAKQVEKHLAEAQP"
     gene            complement(14923..16077)
                     /locus_tag="PKX82_00090"
     CDS             complement(14923..16077)
                     /locus_tag="PKX82_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOF50669.1"
                     /translation="MTRPFDICIRGAGIVGRTLALQLASKRLRVALTKPQPVPEGHSD
                     VRAYALSQPSRALLESVRCWPDEQHATPVLSMVVQGDDQGQVTFSAAEQGVPALNWIV
                     DVPPLEVQLADAVRFQPLIEMVDAPVAAALTVVCEGRTSQTRAEFGVDFEITPYGQSA
                     LAARIQCAQPHQQIARQWFSKGEILAFLPLDGPQGNLCALVWSVSPERAHELKNMDAS
                     DFCQALETASHGALGALTLTSERNVWALQQAQAHRWSGSSAQGAWVLAGDAAHNVHPL
                     AGQGLNLGLGDVAELVRVLDARPYWRSVGDARLLRQYERARKADFAMIGNAGDALQRL
                     FNHSHPALQTLRNLGMNGFEISGPLKHWVTRRAMGNTPTITPPTPTKVLP"
     gene            complement(16129..17877)
                     /locus_tag="PKX82_00095"
     CDS             complement(16129..17877)
                     /locus_tag="PKX82_00095"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOF50670.1"
                     /translation="MFKFFSGLLLSALLASTLTPSAARAQPVMLYDATPSLQLDSPLQ
                     VWVEQGSASTVGVATAIPQNFKPLPALTRHELTTGTTVWIRFSVARGPFDTAQWTLNV
                     PIPYLDSVTLHQRNPDGSWTAQTAGDLTAQSGWSRKGLYPDFDLMLPNSGPQELVLQV
                     RNFKPSAVPVRLVARNERESSRLTELLIMGVALGALIAMAALSTLRFGQHNSRIDGWA
                     ALYALLISATLAQVNGVLNAFVWATMPTVGDYAVSLLPAVALGGTLLFVRQLYALSTH
                     HHRYDRFLSGIAWVAIASIAFHLVLDRTDADRLGSITMILATLAGLIATLLSWRGESS
                     VGRWLVLAYVPQFLGAMYLFAEAMGLVPTLWEMRYVTSLSIAMSVPILVYALSQITHD
                     RRELVIRAKHLPTQDALTGLLTAEVFQNHLEGALMRSINEREPIALVMVSVVNHDYIR
                     SSFGDTTAEQCILRAVIKIQRILRDVDPAGRMDTANFALLMEGMRDRKAVTERMVKLI
                     GSGLIPLPGLMPEVVLHFQAACVLLHENPVPADRVMDDLRALLAEMSSRNRRPVRFLE
                     PIPTEASPLQSELGPD"
     gene            17988..18764
                     /locus_tag="PKX82_00100"
     CDS             17988..18764
                     /locus_tag="PKX82_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOF50671.1"
                     /translation="MMYRSVLAFLIALGCLGTAHAAGPDCARPYTLALHDHGLLYSAQ
                     TGIDKDVADELVRRSGCKVTVSLMARARIWQLIESGALDFSLSGITNANRDTFAAFAW
                     YFSNKYYLLVRKDSGVRSIADFERNPNLHIGVIRSFRYSTSANRLVDQLQAENRLSQA
                     GGLAPSYQTLMLNRIQGMIIEPFDYPTLEEAKIRDTTTIIEFNDPPVPHGLIMSKKAL
                     PEAEQEKWRALIQDMRADGTLRRIFEKYFNPTLAAAMVEF"
     gene            18927..20354
                     /locus_tag="PKX82_00105"
     CDS             18927..20354
                     /locus_tag="PKX82_00105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF50672.1"
                     /translation="MRETVSRVEQRASAYEQMLRGVQALLATTDLTDRKAVHDYVEML
                     QLDANFSAVQVIGVVERIPAERKASHTQVMRKRGFADYTVFPSGERPEYAAVIQRESS
                     IGRQRAAPLGLDVWAEPVRRAAMERARDSGMAAITGKVRLAVDSGIDAPPGFIMYLPL
                     FAPDQPHGTVAQRRANLVGWVFASFRMDDLMASMYGKTAPGIALSIYDDVVPDASTLL
                     YASSAGAEAVSPAATAGLQANEYMVVAGHTWTLVLQAQREFEGRFGGNGATVIAVAGA
                     GLSVSLALLVWLMVTARDRALYFAAEMTEELRMMAQHDPLTSLPNRALFSDRLRGELV
                     RAQRHGGLFAMIFLDLDNFKPINDTYGHGVGDVMLMEVAKRLRATVRTSDTVGRIGGD
                     EFVVLMPELADASVAFGLAEKIRMAVREPMVVEGHALSVSCSLGVAVYPDDGTDEISL
                     AKRADEAMYRAKERGRDGVVRAGSD"
     gene            20425..21516
                     /gene="ychF"
                     /locus_tag="PKX82_00110"
     CDS             20425..21516
                     /gene="ychF"
                     /locus_tag="PKX82_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOF50673.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     EVPDPRMAQLAEIVKPERMVPAVVEFVDIAGLVAGASTGEGLGNKFLAHIRETDATIN
                     VVRCFEDDNVIHVAGKVDPIADIEVIQTELCLADLGAVEKALHRVTKAARSGDKESIK
                     LVAILEKCQAALNEAKPVRSIDFTKEELPLLQQFFLITAKPAMFVANVSEDGFENNPL
                     LDRLKAFADAQKAPVVAICAKLEAEMSEMSDEDRQMFLEELGQTEPGLNRLIRSAYTL
                     LGLQTYFTAGVKEVRAWTIHVGDTGPQAAGVIHTDFEKGYIRAQTIAFEDFITYKGEQ
                     GAKDAGKMRAEGKDYVVKDGDVMNFLFSS"
     gene            complement(21572..22066)
                     /locus_tag="PKX82_00115"
     CDS             complement(21572..22066)
                     /locus_tag="PKX82_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50674.1"
                     /translation="MDFDLETILASLAVVISVVSAYLSRRALDAQISISKNTAFFTQK
                     AAAEEYLAQHPELLELHGIEPRMLDALGITEHEFVYLMQSFNAAELYYQIAESKTITL
                     SQYRKTLLSNPKVKLAWQNLIRGKLLTESAFTKTVDSFYASEDLRPQPEDRSSTSARV
                     LQNK"
     gene            complement(22163..22636)
                     /locus_tag="PKX82_00120"
     CDS             complement(22163..22636)
                     /locus_tag="PKX82_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOF50675.1"
                     /translation="MPAWITPLLDLLSLPEYSLGTVFVVSFISATLLPLGSEPVVFGV
                     VQLNPELFWPVVLVATAGNTLGGALDWWIGYGAHRVADKYHHSKHHMRVLDWLQKLGP
                     KACLLAWLPAVGDPLCAVAGWLKLPFWPCLFYMAIGKFARYVTMTAALTGLVKLW"
     gene            22769..23815
                     /gene="dusB"
                     /locus_tag="PKX82_00125"
     CDS             22769..23815
                     /gene="dusB"
                     /locus_tag="PKX82_00125"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOF50676.1"
                     /translation="MHIGQFALANPLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTS
                     RPDLLNSLKTSRRANHDGEPGPIAVQIAGTEAQMMADAAVYNIDRGAQIIDINMGCPA
                     KKVCNKWAGSALMQDETLAMEIVQAVVNACAPRNVPVTLKMRTGWCQAEKNAVVLARA
                     AEAAGVSMVTVHGRTREQGYKGSAEYDTIAAVKAALSIPVVANGDITTPEKARDVLRA
                     TGADAIMVGRAAQGRPWIFREIAHFLATGAHLAPPLVSEVKRLLVEHLHDHYTLYGEF
                     SGVRSARKHIGWYVRELPGGEHFRAQMNLLEDSEAQVAAVERFMDHLNEKMDRIPAAG
                     AASGMREDTMEMTA"
     gene            23812..24048
                     /locus_tag="PKX82_00130"
     CDS             23812..24048
                     /locus_tag="PKX82_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF50677.1"
                     /translation="MSKKHIEECVRTSLEGYFKDLRGTEPDGMHDMIVRVVEKPLLEV
                     VMHHADHNQSRAAQWLGLNRNTLRKKLLEHKLID"
     gene            24109..>24321
                     /gene="purH"
                     /locus_tag="PKX82_00135"
     CDS             24109..>24321
                     /gene="purH"
                     /locus_tag="PKX82_00135"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905910.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOF50678.1"
                     /translation="MNALLSVSDKTGIVELAQALHAQGIKLLSTGGTAKLLAEKGLPV
                     TEVAEMTGFPEMLDGRVKTLHPRVHGG"
BASE COUNT         4500 a         7190 c         7795 g         4836 t
ORIGIN      
        1 gttgtgtggc ctgcatggcg gtatcggcat tgaggcgaat ggtgttggac agatctgcga
       61 cggaagagga cgtggcttgc aatgcagacg ctgtttgttc ggtgcgggtt gcaaggtcat
      121 tgttgccgat agaaatttca ctggcagccg tgtcgatctg atctgcgcca ctgcgaatat
      181 tggcgatcat gccgttgagg cgttgcgtta cctcacccaa agcgcgcagt acctggccgg
      241 tttcgtcact gcctgcctgc acatcgcgca ggctcagatt gccttcggcc acgtcacgcg
      301 caatcccgac ggccgatttc aagggtcgga caatctcgcg tgcggtaaac caggtgacca
      361 gcatgccaat caccagtgca atcgctgcga gcgtcaggga agccttgtta ccggtggaca
      421 ggctttcggt ggtcaattca ccgctgtgtt tggcctgctc cacttcgccg ttgaattgct
      481 ccgagagcag tccgcgggtt tcctggtagg cggtatcggc tgcgctcata tagatcgcag
      541 cagtcgccag gtctgcgtcc ttcatatcga cggtgtccaa tgcggacttg gtgtaggtct
      601 tgaacgctgc atccagccgg gtcagccgtg acactgcgtc ggcgtcgcct tgcgccatag
      661 cttcgcgcag ccggtcgatg cgctgctggg tcgttttcag ctctgcagca attttttcgt
      721 ccagcgcctt gatgatggcc ggcttcaggc cagcacccgt gtaggccacc gtttgcatga
      781 ccagcccatt gaccgccacc acgcgctcct gtacctccgc caccatgacg acgtggacca
      841 tgctctgctt ggtcaggcgc tccagcgtct gggaggtctc ccgggacgtc cacaggctat
      901 acagtgctac gctcatcagg cacagcagta ccaatgtagg agcgatggcg acctttacca
      961 tgagggatag gcggtcaaaa aacgttctca tgccgtatct ttcaaaaaac agaaaaaccg
     1021 gtgcacctga tacggcgtca ccggtccatg ccagtctggc tgcgggaaaa cgaaattagc
     1081 ggtatgcgcc aaccacgaca aaaccatcaa agccggggat acgtttgacg tagctggttt
     1141 tgccttcaac ttttttggtc acagggtgtg cccagtcgta gtccacccag ccttcgcctt
     1201 tggtggagcc cacttcgatg aattcacgtg caaatccctt gccgttctgg tccttgagtt
     1261 caatcaggtt tttgccgaca agcttctcgt tggcaccatg tgcaagcact acgcccttca
     1321 tgtccatggc gaaaacgtac aaatcctttt tggtccagtt ggctttgtcg gctgtgaaat
     1381 ccttgaatgc ctggtcattt cctgccttct tgacgtgcgc caatgccgca ttgaccatgg
     1441 ccttggccta gtccttggta ccgtggtcct gtgccatagc accgaaggat acaaagccag
     1501 caagcaccag cgtcatcaga ttgcgaaaca gtttcatagt tggtcgtctc cacgaggtta
     1561 aaaaagtact gtgaacaata cgtaacggca atctacgtgg caacttaagc gagcgcaagc
     1621 ctccaaattg cgttttatca ataagagctt ataaaaggcg atcacggacg tccgaagacg
     1681 tcccgttacg acgctcaggc ggtttccagt gccaacaccg ccgtggctgt gttggaaatc
     1741 gggatgtggt aattgctggt cacccgcctc acgctggagc cgccaccggt agccagtgcg
     1801 ccgcgcatgg ccagccacat cagcaactcc actccctggg tgccagtttt ttctaccagc
     1861 tcgtgcacac tgaactgcgt agcccattcg ggattggtgt ccaggctgtc cagaaactgc
     1921 aggtcaaacg ccttgttgat aaagcccgcg cgctcaccgt cgagctggtg gctcaggcca
     1981 ccggaggcaa tcaccgccac ttttttgctg ctgtcccacg agcggatggc ttcgcccacg
     2041 gccttgccca gcgcgtagca gcgcttggcc gatggcagcg gaaactgcac ggtattgatg
     2101 cacaccggca ccacggtcac cgggcagtcg ccttcgggcc aaaacagctt caggggcagg
     2161 gtgcaggcgt ggtccaccag catctcctgg caggtcacca cgtcaaactc cttttctacc
     2221 agggtgttaa tcaggtgcca tgacagatcc acctcgccct tgaagggggg cagcgtggga
     2281 atgccccaac cttcgtcggc gttgttgtac tcgtgcgctg cgcccacggc gaaggtaggc
     2341 atcttgtcca ggaagaagtt caggccgtga tcgttgtaga acatcaccac cacgtcgggc
     2401 ttcttctcgc ccagccattc gcgcaccggt gggaatccgt caaaaaaagg tttccagtac
     2461 gggttctgct gcagaccttt gtggattgcg ttgccgatgg ccgggatgtg ggaggtgccg
     2521 aggccgccaa tgagttgtgc catgtgaatg tgtcctgtgt tgttttatat ttacttgccg
     2581 gccgccacga gcttggcctt gaactcatct ttggtcatgc cggtttgctg ggcaccaata
     2641 tcctgcatgt cgagcttgaa aatgccggca aacttggcca gatagtaggc atttcctcca
     2701 gcggaaatca gctgcagcac gttcttggcg cggatggctg cagcctgcgg ttcggtcagg
     2761 ccgtactttt tcatgtagcc ctcttcatcg gcaagaaagg cggcgcggtt ggcggcatcg
     2821 ttgaacgaga agcacatctt gttcagggca tagccctttt tggcctgttc gccgtcaaag
     2881 ggtgtggtac caggaatttt gggaaggggg gcgctcacga tttgctcctt ttttgtggat
     2941 gtttgacctg catcagccgc agagtatccc gaccccattg ccacaaataa atagatcaat
     3001 gatcatgcat tgcatgagta aattcgattg gtctgatctg gatgcagggc ttctgcaact
     3061 gctggtggcg gtggtggagg cgggtagcgt tacgggcgcc gcccagcggc tgggcgtcac
     3121 acagtcagcg gtgagccacc tgctggacaa gctgcggggc atcacgggcg acccgctgtt
     3181 cgtcaagagc gggcgcggta ttgtggccac cgcgcgggcg gaaagcctgg ccgaacaggc
     3241 gcgggagttg ctgcgccagt tgcagcagtt cgcccatagt gcggagttcg atccggcgcg
     3301 ctggaaaacg tccatcacca tcgccgccaa cgattttcag cgggatctgc tgttacccgc
     3361 tctggcccaa cgcctgcgcc aagccgcacc tttggtgcaa ctgcgggtga ttccctcggc
     3421 cgtgccacgg ctggagaccc tgcgcgccga cgaatgccaa ctggttatca gcccacgccc
     3481 gcccgacggc accgacatcc tgcaaaaacg cttgtttgaa acggcgtacc gggtgttctt
     3541 cgatgccagc cagcgtgcgg cgccgaagac ccaggaggag taccaggcgg ctgaccacgc
     3601 caccgtggcc tacgaggcgc accgggggct ggacatcgac cggcagtttg aagcccaggg
     3661 gctggtgcgc cggtttgccg tggtggtgcc gggcttcagt gccttgccgg cgttcgtgcg
     3721 cggcacgccg ctgctcgtga ccgcgcccgc cttgctggcc aactccacgt ttgccggact
     3781 ggcgcatgca ccggtgccgg tggcctgccc ggccatgccc atgtacctga tctggcatgc
     3841 ccgctaccag caggacccgg cgcaccgctg gctgcgtgag cagctggagg ctgtggtggc
     3901 accagcattg agttccccga agggctccgg tacgatccgg tgatgaaaaa gtcaaaaagc
     3961 ggtaaagagc ccaagcccgg aaaactggac aaaggcgcgt actacgagaa gctcgctgcg
     4021 ttgcagctgg agctcaacga ggtggcgcgc tggctgcagc acaccggcaa gcgtctcgtc
     4081 gtggtgattg agggccgcga tacagcaggc aagggcgggg tcatttccgc catggctgaa
     4141 accttgaacc cgcgccagtg ccgtaccgtg gcattggcca aacccagcga ccaggaaaaa
     4201 acgcagtggt atttccagcg ctacgtgccg cacttgccag gggctggaga gatcgtactc
     4261 tttgaccgga gctggtacaa ccgcgccggt gtcgagcgcg tgatgggttt ctgctccaaa
     4321 gaggaggtca aggcgttcct gaaacaggcg cctgtgtttg agcggatgct ggtggacgat
     4381 ggcgttttac tgttcaagta ttggctcacg gtcgaccagg cgcagcagga ggagcgcttt
     4441 gccgagcgcg cggccgaccc gctcaagcac tggaagctca gccccatcga tgtgaaggcc
     4501 cgcgaaaagt acgccgaata cggacttgcc cgcgacgcca tgctggaggc gacccacaag
     4561 cccaggacgc cgtggacgct ggtggatttc aatgatcaac gtttgggccg attgacgttg
     4621 atccgccact tgctggacca cattcccgat aggcacgtgc cggaatccgt ggtcaccttc
     4681 ccgccgctgg accatgcccc actgcgcgag aagtttggcg cctcgcccaa gccgatcgcc
     4741 cctgcggcct gacgcactcg cagactacaa agggctgcgg ccgcgaatca gacgtaccag
     4801 aatcacgacc acggcgatca ccaggaggat gtggataaaa cctccgagtg tgtacgacga
     4861 gaccaggcca agggcccaga aaatcagcaa taggatggcg acggtttcca gcatctggtg
     4921 ttccttgtag gtagactttt ttcaaaagag cagtctcatc caccggaatc gatccgtcgg
     4981 tgcgctggca cacacccgcg gcccacgcag ccgctttggg ccgcgtcaac gtttgcaatc
     5041 cgggaaatat aggttaaatc gacctccggc ccccgtccag tatgcgcaag cagctatata
     5101 aatagtagca attcaggcca gcatgcgccc ggtctccacg cagcgggcgt gccacgacag
     5161 ggcttcggtc agcaggtggg gcgtgtgttt gccgggtgca tcgcgcaggg cgcgctcgaa
     5221 atagtcgcgc agtgccggtt ggtagtcggg gtgcgcgcac ttctcgatca ccagccgcgc
     5281 ccgctgcgtc ggcgacaggc cgcgcaggtc ggccaggccc tgctcggtga cgatgatttc
     5341 cacgtcgtgt tcggtgtggt cggtgtgcga gaccatgggc acgatgcacg agatggcgcc
     5401 gttcttggcg gtggacggcg tcatgaagaa ggacaggtag gcgttgcgcg caaagtcgcc
     5461 cgagccgcca atgccattca tgatgctcga tcccatcacg tgcgtggagt tgacgttgcc
     5521 atagatgtcg gcctcgatca tcgcgttcat ggcaatcagg cccaggcggc ggatcagctc
     5581 ggggtggttg ctgatctcct gcgggcgcag cacgatgcgc tcgcggtaga agtcgatgtt
     5641 ggcgttgaag tccgccagtg ccgccgggct gaacgacagc gccgtggccg aggccatggc
     5701 gagcttgccc gagcgcagca tgtccagcat gccgtcctgc agcacctcgg tgtaggcggt
     5761 catgttctcg aacggtccct tgttcaggcc gatcagcacc gcgttggcga tattgcccac
     5821 accggactgc aggggcagca actcgggtgg caggcgcccc gctttcactt cacgctgcag
     5881 gaagtcaatg atgtggccag cgatgcgctc ggagttctcg tcaggcgccg caaaggccga
     5941 gttgcggtcg ggttgctgcg tctccaccac tgcaatcacc ttgctcaggt cgcattgcag
     6001 gtagtggtcg ccaatgcgat cgccggggtg gaggagcgga atggggcggc gcagcggggg
     6061 caggcgggtg ccgtagtaga tgtcgtgcat gccctccagg ccaatattct ggtgggagtt
     6121 cacttccaga atgatgtggt ctgcatggtc cagccaggtc ttgttgttgc ccacggagga
     6181 ggacggaatc aggcgcccat cgggcaggat gcccgccact tccaccaccg ccacgttcag
     6241 cgggcccaga aaaccaaacc atgccagctg cgccacatgc gagaggtgga tgtcggtgta
     6301 ttgcgtggcg ccggcattga tctgcttgcg cactgtgggg tcggactggt agggcaggcg
     6361 catggaaatg ccgttcaccg cagccagggc cccgtccagc tctggcgcgg tcgaggcgcc
     6421 ggtccacagg ccgatgcgga agtcttcgcc cgcggcatgc agccgctcaa tgcgccgggc
     6481 cagcgcttcg ggaacctgtt tggggtagcc cgcgccggta aacccgctca tgccgacgtt
     6541 ggcgccagcg ggaatgagtg cggccgccgc ctcggcggac atgatcttgg acaggtaacc
     6601 ggggtgacgc acacgggtct ggttcgacat aaaaaaactg ctccaacggg gtagtgcgcc
     6661 gttcgcacaa aaggtgcgca tcttagcagt gcaacgtaaa atgattaggg taaaccctta
     6721 ttcgtgcgct ttggcgtatc gtggattccc cgtatttatc aagagaggtt gggtatgaat
     6781 agcgaagcca tctggacatt tctgacaacc cagggcgccg acttcggcct gaagctgctg
     6841 ggtgcgctgg ccgcatggat cattggccgc tggctgattg ccatggcgct gcgcctgctg
     6901 aatgcagctt ttcagcgcgg cggcaaggtg gaccagacgc tggccaatta cctcacctcc
     6961 atcctgtcgg tgatcctcaa catcgtcctg attctggcga ttctggatat ttttggtgtc
     7021 aagaccacct cgtttgcggc actgctggcc ggtgcggggc tggccattgg caccgcctgg
     7081 ggtggcctgc tgacgcactt tgctgcgggc gtgttcatgc aggtgctgcg cccctacaag
     7141 gtgggcgatt ttgtaaccgc agggggcatt accggcacgg tgaaggagct gggcctgttt
     7201 ggcaccaccc tggtcacgcc tgacaacgtg gtcaccatcg tgggcaacaa caccgttttc
     7261 tcgggtgcca tccagaattt cagcaccttg ccccaccgcc gggtggattg cctggccaag
     7321 gtggccaaca gcgtggatgt ggtggacgcc attgcgcgcc tgaaagtggc ggtcgctgcc
     7381 attccgaatg tggccaccag tcccgcgccc gatatcgaaa ttttgcagtt cacccccgaa
     7441 ggcccgctgc tgtgcgtgcg cccctacacc tataccgacc actattggca ggtgtacttt
     7501 gacacccaca aagccgtggt cagcaccttt ggcaccgcgg gctacccggt gcccgagaca
     7561 ccggtggtgc accgcaattt atagtcaaat ctggctctag cccccgtgca gtatgcgcaa
     7621 gcagctatcg tttctatagc tgcttttttt atggcgttac aggggtagcg cggccgtttc
     7681 gcgggtcagc acctccgcgc gcagcgtgta gctgcgggtt tccgtgatct tcacgtccac
     7741 cagctggccg accaggcggg cgtcgcctgc aaagttgacc acgcggttgc actcggtgcg
     7801 gcccatcaac tcgccgttgt cgcgtttgga tgccccttcc accaggatgc gctgcaccgt
     7861 gcccaggcgt tgctcgctga tgcgggtgat gttggcattg atggcagctt gcagctcctg
     7921 cagccgtgcc agtttcacct cgtgcggcgt gtcgtcatgc aggttggctg caggtgtgcc
     7981 ggggcggggg ctgaagatga agctgaagct gttgtcaaag ccgatgtcgg cgatcagctt
     8041 catcatcctg gcgaagtcgt cgtccgtctc tccggggaag ccgacgatga agtccgagct
     8101 gatggacatg tccgggcgga tggcgcgcag tttgcggatg gtgctcttgt attccatggc
     8161 ggtgtagccg cgcttcatgg ccatgaggat cttgtcgctg ccgtgttgca ccggcagatg
     8221 caggtggctc accagtttgg gcaacttgtc ataagccgca atcaggctgg gcgtgaattc
     8281 gttggggtgg ctggtggtgt agcggatgcg ctcgatgccg ggaatctcgg ccacgtactc
     8341 cagcagcgtg gcaaagtccg ctatttcgcc gccctctgtc atgggcgcgc gccaggcatt
     8401 cacgttctgg cccagcaggt tgacctcttt cacaccctga tcggccaggc cggcaatttc
     8461 caccagcacg tcctcgaacg ggcggttgat ttcggtgccg cgggtgtagg gcaccacgca
     8521 gtagctgcaa tatttgctgc agccttccat gatgctcaca aaggcgctgg cgccttccac
     8581 cttggccggc ggcaggtggt cgaacttctc aatctctggg aagctgatgt ccacttgcgg
     8641 ccggttctgg gctttgcgag cgttgagcat ctcgggcagg cggtgcagcg tctgcgggcc
     8701 aaacaccacg tccacatagg gcgcacgctt gatgatctcg gcgccctcct ggctggccac
     8761 acagccgccc acgccaatct gcactccctt ggctttgagg tgcttgatac gacccaggtc
     8821 ggaaaacacc ttctcctgcg ctttctcgcg caccgagcag gtgttgaaca gaatcaggtc
     8881 agcctgctcc acatcgtcgg tgggctcgta gccctgcgct gcgcccagta cgtcggccat
     8941 cttgtccgag tcgtactcgt tcatctggca gccgaaggtt ttgataaaga cttttttgga
     9001 catggcgggt cctgcaatgc tatgaatttg agagctgctt gcgcagaaga tacgggggct
     9061 agagccccaa aagacctaaa atccagcccg gaattactgg gcggcggacg aatacgaagg
     9121 cgtagctgga tagagcttgg cttcgatgtc ggtgaggacc cagacctcat gcagcatgcc
     9181 ttgcgactcg cgcagggtca tgaccggcag ggtctggccc accagcgagg ccgacatggc
     9241 cagcatgcca ttggcgccgc ggatgcgtgc accgggggat aggcgatcga ttctgttgtt
     9301 cagcaccact tcagggggcg ccacgaccat cagcgtgccg cgcacggctt tgggcggaaa
     9361 ggtgcgtacc gccacggaca cggtgccggt ggtgtccgtc agcacctggg tggtggggct
     9421 ggtctgggcc gacgccgcca gaggcagtgc ggtcagcagg caaagtaggt tcaaagcgcg
     9481 aaggcagcgg ttcatggtgt gtatccagag gcttgcttgt gggaggtgaa tgctaactca
     9541 aaacacttcg gtgtcgactt cgctattgcc ctgcgggttg tagcggctgc ccaccacgtt
     9601 gccggagtgg atcaacgcct ccaggcgggc tatggcgtct gcgctgagtt ggacgtcagc
     9661 tgcacccagg tcgtcgtgca ggtggtccac gcgggtggtg ccggggatcg ggatgatgtg
     9721 cggcgccttg tgcagcagcc aggcaatggc caactgggcc ggggtgcagt ccatctcttg
     9781 cgccagcgcg cggtaggcgg gcagcagggc caggttggcg gcgtagttgt cgggtgcaaa
     9841 gcgcggcatg ctgcggcgga tgtctttggc gtccagagtg tccacatcgg ccaggtcacc
     9901 acacaaaaag ccgcgcccca ccgggctgaa ggccacaaag gcgatgccca gctccttgca
     9961 ggcatcgagc accgcaatct cggggttgcg cgtccacagc gaatactcgg tttgcagggc
    10021 cgtgatgggg tgcacagcat gggcgcggcg gagcgtatcg gctgacacct cggataaacc
    10081 aatgctgcgg atcttgcccg cgcgcaccag atcggccagg gcgcccacgc tgtcttcaat
    10141 gggcaccttt ttgtcccagc ggtgcaggta atacaggtcg atcacgtcgg tgcgcaggcg
    10201 gcgcaggctg tcctcgcact gctggcggat ggtctcgggc cggccatcga tcacccggac
    10261 caatttgccg tcgcctgcca catccacccc gcccatgccg cacttgctgg ccagggtgaa
    10321 acggttgcgg tgcggctgca tcaccttgcc gaccagggtt tcgttggcac caaagccgta
    10381 gagcgtggcg gtgtcaaaca gggtgacgcc agcatccagc gcttccagaa gtacgcgctc
    10441 accttgctcg gccgataccg gcgcgccgta ggcgtggctc aggttcatgc agcccaggcc
    10501 gatggcgctg acctggaaag ggcctaaaga tcgttgttgc atgttggact cctgcgagaa
    10561 agctggacaa gcgccaaaca aaaaacccgc actagcgtgt gctggtgcgg gtttttgctt
    10621 gtttggtggt cgtaggtgga tttgaaccac cgacatcagc attatgaatg ctgcgctcta
    10681 accagctgag ctatacgacc gaaagaccag cattatagcg gcataaatta ccgtgtcact
    10741 ggtgtttgaa attggcgggg cgtttgttca cgaacgcgtc catgccttcc ttctggtcgg
    10801 ccgtggcaaa aagggcgtgg aacatgcggc gctcgaacat gattccgtcg gccagcgtac
    10861 cttcgaacga gcggttgaca gcttccttgg cggccatggt ggccatttgc gagaaaccgc
    10921 aaatcagcag ggccgcaccc agggcttctt cttgcagctt gtccagtggc acgacgcggc
    10981 tgaccagccc ggagcgctcg gcttcggtgg cgtccatcat gcggccggtc agggccaggt
    11041 ccatcgcctt ggacttgccg atggcgcggg gcaggcgctg cgtgccaccg gcaccgggaa
    11101 ttacgcccag tttgatctcg ggctggccaa agcgggcgtt gtcggccgca atgatgaagt
    11161 cgcacatcat ggccagctcg cagccgccac ccagggcaaa gccgctcacc gctgcaacga
    11221 tgggtttgcg gatgctgcgg atgacttccc agttgcgggt gatgtagtcg cccttgtaca
    11281 cgtcggcaaa ggtgtacgtg gccatggcgc cgatatcggc accggcggca aaggcttttt
    11341 cgctgccggt gatgaccatg caaccaatcg ccgggttcag gtcaaagccc ttgagcgcgt
    11401 ggcccagctc gtccatcagc tggtcgttca gcgcattgag ctgcttgggt cggttcagcg
    11461 taatgatggc aaccttgtcg ccttcgatac gggtggtgat gcattcgtag ttcatgatgg
    11521 tgctgttgaa gttggtatcg tcgaaacatc cactatagcg aagaggcact gcacatggct
    11581 gatcccactg ttttgtttgc ggaccacggg aatgtggccc tggtgaccct gaaccgcccg
    11641 gacgccctca acagcctgac ccaccagatg caccgggatc tgtgggcggc gctggaccgg
    11701 gccgaggcca atccggccat ccgggcgctg gtgctgaccg gcgccgggcg cggtttctgc
    11761 gccggtgcgg atctccatgg gtcggatctt gcccccagcg atggcgccgc cgggcggccc
    11821 gatcccgggg tgacgattga gcagaacttc aaccccacga cgcggcgcat gcagaacctg
    11881 cgcatgcctt gcgtcgcggc cgtcaacggc gtggcggcgg gtgccggtgt gtcgctggcg
    11941 ttagcgtgcg acgtgacgat tgcggcgccc ggtgcgcgct tcatccaggc cttcagcaag
    12001 atcgggctga ttcccgatgc gggcagcagc tggttcatgc cgcagcgcct cgggctggca
    12061 cgggccatgg cgctggccat gacgggggac gcgctcagtg cgccacaggc caaggaatgg
    12121 gggttgatct gggatgtggc ggacgactgt gtggcggccg cgctggccta tgcgcagcgg
    12181 ctggcggaca tgcccacgct ggcgctggta gcgacacggc atctgttgcg cgagtccacc
    12241 gcgcggacgc tgaaccagca gctcgatgtg gagcgcgata cgcagtcagc gatgggcaag
    12301 acccatgact atgtggaagg ggtgacggcg tttcgcgaga agcggtcggc ccggttcaac
    12361 ggaacctagg cgccaccgtc cagccacttg ttggccgccg ctgcatcgcg caccgacagg
    12421 cgcacggcct ggctgggttt gggcatggtc agcgacaggg tcatcaggcg tttgtcgcgg
    12481 gcaaccagcg cggtgacctt cttggccttg ccggtgtaga tgttcaggtc gtccagggtc
    12541 tgcatgcgcc aggggccgct ctcggcttcc accgcaatcc actcatcacc ggcggcgaag
    12601 ccagctttct cggccacgct ggcgcgcagc acctgctgga caaacagacc gccggattcc
    12661 ttgacgcgca ggcccagttg ctgggcaatc tggtcgggtt cgcggttgac catcaggccg
    12721 tgggcttcca gtagttcggt caggggcagg tctttggtac tgtgcaccca gcgggccagg
    12781 tctttggcgt aggcgcggcc gccgagttcg ctgagcacgg ccagcaggtc gtcctgggcc
    12841 atggggccgc cggcacaacg cttccacagg ccgcgcatca cctcgtccag cgtggtcttg
    12901 ccttcggcac gcagggtcag gtccaggcac agggccacca gagagccttt ggtgtagtag
    12961 ctcaccgtgg cgttaggggt gttttcgtcc tggcggtagt actttaccca ggcatcgaaa
    13021 ctggcctgcg ccacggtctg caccagacgg ccaggcgttt gctgcacctg gttgatggtc
    13081 ttggacagca gcttgaggta gaccgtgttg tcgatcagct tggcgcggcg cagcagcagg
    13141 tcgtcgtagt agctggtaaa gccttcgaag aaccacagta gttcggtgta gttctcctgc
    13201 gtgtagtcgt agcgggaaaa ctcggccggc cgcagacgtt tgacgttcca ggtgtggaag
    13261 tactcgtggc agatcaggcc catcagggtg gtgtacccct cgggttgctt gtgggtggtg
    13321 ggggccagcg tggtgcgggg ctggcgcggc aggtccttgc gggtgcacag cagcacggtg
    13381 gagttgcggt gctccagtcc gccgtatcca tcgtgcgtgg cattcaggat aaacaggtag
    13441 ttttggaacg gaatcggccc cttgcctccg ccggtagtgc gtgaggcgct attgttttta
    13501 tagttttttc cgtgccagaa gcggatgcct tcttcgcaga tggtctgcgt gtccttgagc
    13561 agctgtgtcc cgtcgaagct cggcggcgca cctgccacca caaagcggtg gggaatgcca
    13621 caggcctgga aatgcccctc ccagaaatca cccagttcca ccgggcaatc cgccagcatg
    13681 tcgtagttgc tggcgctgta gcggccaaag ccgcgtttgt cggtgcgcac ggggggcagt
    13741 ccggtggcca ctttccattg gggcgaatcg gtgcaggcca ccaggtcgac ggtgtgcgtt
    13801 ttgtcctgct ggccttcgac catcaggaac aggctggtgc cgttgaaaaa gccccgggtc
    13861 gcgtccagcc aggcggtgcg caccgagttg tcaaaagcgt aaacctcata ctgcagttcc
    13921 agtggcgcat gggctgtgca gtccacttgc cagcgtgcct tggtcagttg ctggagctcc
    13981 acggcgcgac cggattggtt cgcgcgcagg ttttgcaggt tcttggcgaa ttcccggacc
    14041 aggtaactgc cgggaatcca cgctggcaac tgcacatgct gctgtgccgc gggctgtgcg
    14101 atggtcagtg tgacctggta caggtgggca tgcaggttga ccatttccac ccggtagtgg
    14161 atgtctgccg ggctgccagc gcgaccggcc gggctgcgtg tcatggctgg gcttcggcca
    14221 gatgtttttc gacctgtttg gcatcaatag cgccgggtac gcggctgccg ttgacaaaca
    14281 ccagggtggg cgtgccggta attttgtagg cgcggcccag ctccacattg cgggccagcg
    14341 cgctggtgtc acacatggct gcggctgagg gtggtgcctg gtcgcgcacc atccagtcct
    14401 gccaggcagc agtcttgtct ttggcacacc agatggcttt ggatttttct gccgaatcgg
    14461 cgcccaggat cgggtatagg aacatgtgga tggtgacgtt gtccactttc tgcaggtccc
    14521 gctcaaagcg cttgcagtag ccacagttgg gatcttcaaa caccgccagt ttgcgcttgc
    14581 cattgccacg cacgatggtg aacgcgtcct tcagtggcag ggcatcgaac ttgatggcag
    14641 tgagtttgtc cacccgctct tcggtcaggt tgcggcggtt gcgcacatcg atgatgttgc
    14701 cctgcaccag gtagttggcc tgggcgtcgg agtaatagat ctcggtgccg ttgacccgga
    14761 tttcgtacag gcccgggatc accgacttgg tgacttcgtc aatcgccttg agttgcggca
    14821 cgcgttcggc cagatttttg cggatgacgg cctcctgagc tccggcgccc agtgcagcca
    14881 tggccagggt ggtgcccagc agcgcgggga aaaaacgtga aatcatggaa gtaccttggt
    14941 tggtgtaggg ggtgtgatgg tgggggtgtt ccccatggcg cggcgtgtca cccagtgttt
    15001 gagggggccg ctgatctcga atccgttcat gcccaggttg cgcagggttt gcagggcagg
    15061 gtggctgtgg ttgaagagcc gttgcagtgc gtcgcccgcg ttgccaatca tggcaaagtc
    15121 ggccttgcgt gcgcgctcgt actggcgcag caggcgggca tcgcccacac tgcgccagta
    15181 ggggcgcgcg tcgagcaccc gcacgagttc ggccacgtcg cccaggccca ggttcaggcc
    15241 ctggcccgca aggggatgca cattgtgggc cgcatcgccg gccagcaccc aggcaccctg
    15301 ggccgagctg ccgctccagc ggtgcgcctg cgcctgctgc agggcccaca cattgcgctc
    15361 gctggtcagc gtcagcgcac ccagtgcgcc gtggctggca gtttccagcg cctggcagaa
    15421 gtcacttgca tccatgtttt tcaattcgtg ggcccgttca ggactgacag accacaccag
    15481 cgcgcacaag ttcccctgcg ggccgtcgag cggcagaaat gccaggattt ctcccttgct
    15541 gaaccattgc cgtgcaatct gctggtgcgg ctgtgcgcat tgaatccggg cggccaaggc
    15601 ggactggcca taaggcgtga tttcaaagtc cacgccaaac tcggcccgcg tctggctggt
    15661 acgcccttcg cacaccacgg taagcgctgc agcgaccggc gcgtccacca tttcaatgag
    15721 aggctgaaag cgcacggcgt ctgccagctg gacttcgagc ggaggcacat cgacgatcca
    15781 gttgagggct ggcacgccct gctctgccgc cgagaacgtc acctggccct ggtcatctcc
    15841 ctgcaccacc atggacagca ccggtgtggc atgctgctcg tccggccagc agcgcaccga
    15901 ctccagcaac gcgcgcgacg gctggctcag tgcgtaggcg cgtacatcgc tgtggccttc
    15961 ggggaccggc tggggtttcg tcagtgccac gcgcagccgc ttgctggcca gttgcagtgc
    16021 cagggtgcgt ccgacgatac cggcgccccg gatgcagatg tcaaagggtc gtgtcatccg
    16081 ggcattgtaa aaggcgcagc acaaccggcg tgccgtgtgg gcttggcgtc agtccgggcc
    16141 caattcactc tgcaggggtg acgcctcggt tggtatgggt tccagaaaac gcaccgggcg
    16201 ccggttgcgt gacgacattt cggccagcag ggcgcgcagg tcgtccatca cccgatccgc
    16261 aggcaccggg ttttcatgca gcaacacaca ggcagcctgg aagtgcagca caacttcagg
    16321 catcaggccg ggcaggggaa tgagtccgga gccgatgagt ttgaccatgc gttccgtcac
    16381 ggccttgcgg tcccgcatgc cttccatcag cagcgcaaaa ttggcagtat ccatgcggcc
    16441 ggccggatcc acgtcgcgca ggatgcgctg gatcttgatc accgcgcgca gaatgcactg
    16501 ctcggcggtg gtgtcgccaa aggaactgcg aatgtagtca tggttcacca cactgaccat
    16561 gaccagcgca atgggttcgc gctcgttgat ggatcgcatc agcgcccctt ccaggtgatt
    16621 ctggaatacc tctgcggtca gcaggccggt cagcgcatcc tgggtgggca gatgtttggc
    16681 ccgaatcacc agttccctgc ggtcgtgggt gatctggctc agggcgtaca ccagaatcgg
    16741 gaccgacatg gcaatgctca gcgaggtgac atagcgcatt tcccaaagcg tgggcaccag
    16801 ccccatggct tccgcaaaca ggtacatcgc ccccaggaac tggggcacat aggccagtac
    16861 cagccagcgg ccaacggacg attcgccacg ccagctcagg agggtcgcga tcagcccggc
    16921 cagggtcgcc aggatcatgg tgatggagcc cagacggtcg gcatcggtac ggtccagcac
    16981 gaggtggaag gcgatcgatg caatggccac ccaggcaatg ccagacaaaa agcggtcgta
    17041 gcggtggtgg tgtgttgaca gggcatacaa ctggcgcaca aagagcagcg tgcctcccaa
    17101 tgccacggca ggaagcagac tgacggcgta gtcgccaacg gtgggcatag tggcccatac
    17161 gaaggcgttg agtaccccgt tgacctgtgc caaagtggca gaaatcagca acgcatacag
    17221 ggcggcccat ccatcgattc tgctgttgtg ctggccaaag cgcagggtgg acagagctgc
    17281 catcgcaatc agggccccca gcgccacacc catgatcagc aactcggtca ggcggctgga
    17341 ctcgcgttca ttccgggcga ccaggcgcac cggtacagcc gaaggtttga agttgcgaac
    17401 ctgcagcacc agctcttgcg gaccagaatt gggcaacatg aggtcgaagt ccggatagag
    17461 ccccttgcgg ctccagccac tctgcgctgt caggtcaccg gcagtctgcg cagtccagga
    17521 gccatctggg ttgcgctggt gcagcgtcac agagtccagg tagggaatgg gcacgttgag
    17581 cgtccattgc gcggtgtcga aggggccgcg ggcgacagaa aaccggatcc acaccgtggt
    17641 gcctgtggtc agttcgtgcc gggtcagggc gggcaggggt ttgaagttct gggggattgc
    17701 cgtagcaact ccaacggtag aagccgagcc ctgctccacc cagacttgca gtgggctatc
    17761 caactggagg gatggcgtcg cgtcgtacag catgaccggt tgtgcccggg ccgcagacgg
    17821 ggtcagggtt gaggcgagga gtgcgctcag caacagaccg gagaagaatt tgaacatgtg
    17881 cagaagccag ggccgtacga tctgcaggcc gcaccgcatc gcgtatttca cgcgagttcg
    17941 gtatcatgca atgcaacagc ccttcacacc tacctccgcc aggccagatg atgtaccgca
    18001 gtgttcttgc ctttttgatc gcgctcggtt gccttggcac cgcgcatgcg gctggcccgg
    18061 attgtgcgcg cccctatacc ttggcgttgc acgaccacgg actgctgtac tctgcgcaaa
    18121 ccggcatcga caaggatgtg gcggatgagc tggttcgccg cagtggttgc aaggtcacgg
    18181 tcagtctgat ggcgcgggcc cgtatctggc agctcattga gtcgggggca ctggatttca
    18241 gcctgtccgg aatcaccaac gccaaccggg atacctttgc agcgtttgcc tggtacttca
    18301 gcaacaagta ctacctgctg gtccgcaagg attctggggt acgcagcatt gcagacttcg
    18361 agcgcaatcc gaatctgcac atcggggtga tccgcagttt tcgctacagc acatcggcca
    18421 accgcctggt ggaccagctg caggcagaaa accgcctcag ccaggcggga gggctggcgc
    18481 cgtcgtacca gaccctgatg ctcaaccgca tccagggcat gatcatcgaa ccctttgact
    18541 atcccacgct ggaagaggcg aagatccgcg acaccacgac gatcatcgaa ttcaacgacc
    18601 cgccagtccc ccacggcctg atcatgtcca aaaaagcgtt gcctgaagcc gagcaggaga
    18661 aatggcgcgc gctgatccag gacatgcgtg cggacggtac gctgcgccgc atcttcgaga
    18721 agtatttcaa tccgaccctg gctgctgcca tggtggagtt ctagcgcgtc ctggacggtc
    18781 gctggttgta tttgtccatg cgcttgccac gccaactttt gctgccctgg ttcattctgg
    18841 tggtgatgct cgggctgacg tggctggtgt gggaccacga gcggcagatc gcgcgcaagg
    18901 aaatgtagtc ccagttcgat ttcgtattgc gtgaaactgt cagtcgtgtt gagcagcggg
    18961 cgtcggccta cgagcagatg ctgcgggggg tgcaggcgct gctggccaca accgacctga
    19021 cagaccgcaa ggcggtgcat gactatgtgg agatgcttca gctggatgcc aatttctcgg
    19081 ccgttcaggt catcggcgtt gtcgagcgga ttccggcaga acgcaaggcc tcgcataccc
    19141 aggtgatgcg caaacgcggg tttgccgact acaccgtctt tccttcaggg gagcgacctg
    19201 aatatgccgc ggtgatccag cgcgaatctt cgattggacg ccagcgcgcc gcgcccttgg
    19261 ggctggatgt gtgggccgag ccggtgcgtc gcgctgcgat ggagcgggcg cgggactccg
    19321 gtatggctgc catcaccggc aaggtgcggc tggcggtgga ctccggaatc gatgcgccac
    19381 ccggattcat catgtacctg ccgctgtttg cacctgatca gccgcacggc acggtggcgc
    19441 agcggcgtgc caacctggtt ggctgggtct ttgcctcgtt tcgtatggat gatttaatgg
    19501 ccagcatgta cggcaagacg gcacccggaa ttgccctgtc catctacgat gacgtggttc
    19561 cggatgcctc gacgctgctc tatgcctcgt ccgccggtgc agaggcggta tcaccggcag
    19621 cgacggccgg cctgcaggcc aatgaataca tggtggttgc aggccatacc tggaccctgg
    19681 tgctgcaggc ccagcgcgaa ttcgagggcc ggtttggcgg caacggtgcg accgtgattg
    19741 cggtggcggg agcgggcttg agcgtgtcgc tggcgctgct ggtctggctg atggtgaccg
    19801 cccgggaccg ggccctgtac tttgcggcag agatgacgga ggagttgcgc atgatggcgc
    19861 agcacgatcc cctgaccagc ctgcccaatc gcgccctgtt cagcgaccgg ctgcggggcg
    19921 aactggtacg ggcacagcgc cacggcggcc tgttcgccat gatctttctg gacctcgaca
    19981 acttcaagcc catcaacgac acctacgggc atggtgtggg cgatgtgatg ctgatggagg
    20041 tggccaagcg cctgcgggcc acggtgcgga cctccgatac ggtgggccgc attggcgggg
    20101 atgagttcgt ggtgctcatg cccgagctgg ccgacgcgtc agtcgcattt ggcctggccg
    20161 aaaaaattcg catggccgtg cgcgagccca tggtggtgga ggggcatgca ctcagcgttt
    20221 cctgcagcct gggggttgcc gtctacccgg acgatggaac ggacgaaatt tccctggcca
    20281 agagggcgga cgaggccatg taccgcgcca aggaacgtgg acgtgatggc gtggtgcgcg
    20341 cgggcagcga ctgagccgga ccacgtgccc tgcggtgagg atttgccact cattacaatt
    20401 ggcgcctgat ttgtaaggat ttccatgagt ttgaagtgcg gcatcgttgg cttgcccaac
    20461 gtcggcaaaa gtaccctgtt caacgccctg accaaggccg gcattgctgc cgagaactat
    20521 ccgttttgca ccattgagcc caatgtgggc atcgtcgagg tgcccgaccc gcgcatggcg
    20581 caactggcgg agatcgtcaa gcccgagcgc atggtgcctg cggtggtgga gttcgtggac
    20641 attgccggcc tggtggcagg cgccagcacg ggcgagggcc tgggcaacaa gtttctggcc
    20701 cacatccgcg aaaccgacgc caccatcaat gtggtgcgct gctttgaaga cgacaacgtg
    20761 atccacgtgg ccggcaaggt cgatccgatt gccgacatcg aagtgattca gaccgagctg
    20821 tgcctggccg atctgggtgc ggtggaaaaa gccttgcacc gcgtgaccaa ggcggcccgc
    20881 tcgggtgaca aggagtcgat caagctggtg gccattctgg agaaatgcca ggccgcactg
    20941 aacgaagcca agccggtgcg ttcaatcgac ttcaccaagg aagagttgcc cctgctgcaa
    21001 cagttcttcc tgattactgc caagcccgcc atgtttgtgg ccaacgtgtc ggaagacggc
    21061 tttgagaaca acccgctgct ggaccgcctg aaggcttttg ccgatgcgca aaaagcgcct
    21121 gtggtggcga tctgcgccaa gctcgaagcc gagatgtcgg aaatgagcga cgaagaccgc
    21181 cagatgttcc tcgaagaact cggtcagacc gagcctggcc tgaaccgcct gatccgctcg
    21241 gcctacacct tgctgggcct gcaaacctat ttcaccgccg gtgtgaaaga agtgcgcgcc
    21301 tggaccatcc acgtgggcga caccggcccg caggcggccg gcgtgatcca caccgacttc
    21361 gagaagggct acatccgcgc ccagaccatc gccttcgagg acttcatcac ctacaagggt
    21421 gagcagggcg ccaaggatgc cggcaagatg cgcgctgaag gcaaggacta cgtcgtcaag
    21481 gatggcgatg tgatgaactt cctgttcagc tcctgacccc agatttatac gccaaattag
    21541 gctctagccc ccgcgggcat tgcgcaagac gctacttgtt ttgtagcact ctcgcgctgg
    21601 tgctggaccg atcctcgggt tggggccgca gatcttcgct ggcgtagaaa ctatcgacgg
    21661 tcttggtaaa cgcgctctcg gtcaaaagct tgccacggat caggttttgc caagccagct
    21721 ttaccttggg gttggatagc aatgttttcc tgtattggct cagcgtgatc gtcttggatt
    21781 cggctatctg gtagtagagt tcggctgcgt taaaggattg catgaggtag acaaactcat
    21841 gttcagtgat acccagcgca tcgagcattc gcggctctat cccatgcagt tcgagcagct
    21901 ctggatgctg tgcgagatat tcctcggcag cagctttctg ggtgaagaag gcggtgttct
    21961 tggagatgga tatctgggca tcgagcgccc gtcgtgacag ataggccgac accacagaaa
    22021 tcacgactgc cagcgaggcc agaatcgtct caagatcaaa gtccataggt aacaccccgc
    22081 acatttcaga cagccgggaa tataccttcc ggtatcgctc ggcaaagggc agaccgcacc
    22141 tcccaagtga aacctcgcac actcaccaaa gcttgaccaa ccccgtcagt gccgccgtca
    22201 tcgtcacata gcgcgcgaat ttgccgatgg ccatgtagaa caggcagggc cagaagggca
    22261 gcttgagcca acccgctact gcacacagcg gatcgcccac cgccggtagc caggccagca
    22321 ggcaggcctt ggggcccagt ttctgcagcc agtccagtac cctcatgtgg tgtttggagt
    22381 ggtggtattt gtcggccacc cggtgcgcac cgtaaccgat ccaccagtcc agtgcaccac
    22441 ccaatgtgtt gcccgcagtg gcgaccagga ccaccggcca gaacagttcg gggttgagct
    22501 ggaccacacc aaaaacgacc ggttcggagc ccagcggtag cagcgtggcg gagatgaatg
    22561 agaccacaaa caccgtaccc aggctgtatt cgggtagcga caacagatcg agcagggggg
    22621 tgatccaggc gggcatagcg cgcagtgtag gcgcagaagc catgccggta gatgcagatt
    22681 tcaattgctg aaattttggg cagctagaat ggagcccggc tcgcgccacc ctgtctgttc
    22741 acactcccat ttttgatcac gcaagcacat gcacattggc cagttcgctt tggcgaatcc
    22801 cctgtttgtt gcgcccatgg ctggcgtgac cgaccgccct ttccggcagc tgtgcaagcg
    22861 cctgggggct ggctacgcgg tgagcgagat ggtgacgtcg cggccggacc tgctgaattc
    22921 cctcaagact tcgcgccgcg ccaaccacga cggtgagccc ggccccatcg cggtgcagat
    22981 cgccggcacc gaggcgcaga tgatggccga tgccgccgtt tacaacatcg atcgaggtgc
    23041 acagatcatc gacatcaaca tggggtgccc ggccaagaag gtctgcaaca aatgggcggg
    23101 ctctgcgctg atgcaggacg agactctggc aatggagatt gtgcaggccg tggtgaatgc
    23161 ctgcgccccg cgcaatgtgc cggtcacgct gaagatgcgc accggctggt gccaggcgga
    23221 aaagaatgcc gtggtgctgg cccgtgcggc cgaggcagcg ggcgtgtcca tggtgaccgt
    23281 ccatggccgc acccgcgagc agggctacaa aggcagcgcc gagtacgaca ccattgcggc
    23341 ggtgaaggcc gcgctctcca ttcctgtagt ggccaatggc gatatcacta cccccgagaa
    23401 agcgcgcgat gtgttgcggg cgaccggtgc ggatgccatc atggtgggtc gcgcagccca
    23461 gggacgcccc tggattttcc gtgagatcgc ccatttcctg gccaccggcg cgcatctggc
    23521 gccaccgctg gtgagcgagg tcaaacgcct gctggtcgag catttgcatg accactacac
    23581 gctgtatggt gagttcagtg gcgtgcgttc tgcacgcaag cacattgggt ggtacgtgcg
    23641 tgaactcccg ggaggcgagc actttcgtgc gcagatgaac ctgctggaag acagcgaggc
    23701 gcaggttgcg gcagtggagc gctttatgga tcacttgaat gaaaagatgg atcgcattcc
    23761 ggccgcgggt gcggccagtg gcatgcggga agacacgatg gaaatgacag catgagcaag
    23821 aagcacatag aagagtgcgt gcgcaccagc ctggaaggct atttcaagga tctgcgcggt
    23881 accgagcccg atggcatgca cgacatgatc gtgcgcgtgg tggaaaaacc gctgctggag
    23941 gtggtcatgc accacgccga ccacaaccag tcccgcgctg cgcagtggct gggcctgaac
    24001 cgcaataccc tgcgcaaaaa gctgctggaa cacaaattga tcgattgagc ggtttccctg
    24061 cgaactccgc gccgcccccc cgttttttaa cttggccctc ccattcctat gaacgcactt
    24121 ctttctgtct cggacaaaac cggcatcgtc gaactcgccc aggcgctgca cgcgcagggc
    24181 atcaagctgc tctccaccgg cggcaccgcc aaactgctgg ctgaaaaagg tctgccggtg
    24241 accgaggtgg ctgagatgac gggctttccc gagatgctcg acggccgtgt caagaccctg
    24301 cacccgcgcg tgcacggtgg c
//
LOCUS       DAOSJM010000002        24045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_2337_length_24045_cov_5.081992, whole genome shotgun sequence.
ACCESSION   DAOSJM010000002 DAOSJM010000000
VERSION     DAOSJM010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 24045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24045
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2337_length_24045_cov_5.081992"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PKX82_00140"
     CDS             complement(<1..492)
                     /locus_tag="PKX82_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOF50679.1"
                     /translation="MNAPLSRDLPEHIRKALETVTLDDKYSLEYGQAFMSGVQALVRL
                     PMLQRVRDMRVGKNTAGFISGYRGSPLGTYDQSLQKAEKYLKAHHVVFQPGVNEELAA
                     TALWGTQQLGFAPPGSNKYDGVFGIWYGKGPGVDRCSDVFKHANMAGTTPWGGVIAVA
                     GDDH"
     gene            609..1121
                     /locus_tag="PKX82_00145"
     CDS             609..1121
                     /locus_tag="PKX82_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOF50680.1"
                     /translation="METLDRFDIAILNELQNDGRLSNAELASRVGLSAAPCWRRVKAL
                     EESGFITGYRAEINRHKTGLGVLAFVRLDADRNSGDVLQALENAIRTIPEVISCHYIS
                     GNGTFELQVVSRDLNTFSQFAREVLINLPNVKDLHTSFSLGEVKASSALPLGHLSPAS
                     SAATTTRGRS"
     gene            1118..1792
                     /locus_tag="PKX82_00150"
     CDS             1118..1792
                     /locus_tag="PKX82_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50681.1"
                     /translation="MKKRLLLVFMCFWPLAPVSIAQLATKSIANPNPLPLRNLLIEVR
                     QVQSGSAQQSGMETGGAARIGNDGQLSMQGQLQARQQQSQQGATAIQQVMVLNGRNAH
                     IALRTAQPLRLLQSFVRNGRVVVTQSVVFLEAGTGFLATPRWDGAELVELSIAAQQAL
                     PNASRITGATSTASTESVLLVPLGQWTPIAQSDLSASNDTQSLGGNSASAMQSSTEVQ
                     VRITVR"
     gene            complement(1805..2764)
                     /locus_tag="PKX82_00155"
     CDS             complement(1805..2764)
                     /locus_tag="PKX82_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOF50682.1"
                     /translation="MSKTSVMRDIDERTNLASNSMFELLLFRLGESPATHKRELFGIN
                     VFKVREIMVMPEITAMVNAPPSVMGVANIRGQMIPVVNLPLITGCNPTKGLAILLVTE
                     FARTTQAFAVEEVNEIVRLEWKQVLSAEGTGGGLVTSIARLDGNAENTRLAQVLDVEQ
                     ILRDVFPEQHKSLDESAVMPKVSIPPGSVILAADDSAVARMMIEQGLQAMNVQYVMTK
                     TGKEAWDKLQAMQVEATAAGKSIRDKVALVLTDLEMPEMDGFTLTRNIKQDPRFAGIP
                     VIIHSSLTGTTNEGHVKSVGADAYVAKFVAEELGATIRSVLNR"
     gene            complement(2829..3998)
                     /locus_tag="PKX82_00160"
     CDS             complement(2829..3998)
                     /locus_tag="PKX82_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOF50683.1"
                     /translation="MRDSFNRRLWVGCSVAAVVAVAGILAGCGSAPMVYPTAPPAPVA
                     RHVVVPAPIPSGPGGSLTQPRSRWVPVAWSELPGFGQDNLFEAWNAWLKSCEKPVAPM
                     GALCGEVRRLSIADAQAQRDWMQARLQPYRVESAQGESTGLLTGYFEPQLEASRVPTA
                     TFTVPLYQPPLSLGQRKPWYTRQEIDTLPEAQAALQGRAIAYLADPIDALVLQIQGSG
                     RLRIAQPDGSQRWIRVAYAGTNDQPYRSVGRWLLDQGLVRDASWPGIKTWLAQNPQRR
                     QELLWSNPRMVFFKEEPLSDLDAAFGPKGAQGVALTPGRSIAVDPGSIPYGTPVWLVS
                     SGPQVSLQRLVLAQDTGTAITGAIRADYFAGWGAEAADLAGRLRQPLQLWTFWPK"
     gene            complement(3998..4828)
                     /locus_tag="PKX82_00165"
     CDS             complement(3998..4828)
                     /locus_tag="PKX82_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOF50684.1"
                     /translation="MKIAAIQMVSTGDLNDNLQQAAALLDQAAAQGAELAVLPEYFCL
                     IGQNDADKLAIQEPYGKGPIQQFLADTARRLKLWIVGGTLPLTATQADRVFNSTLVFN
                     PQGLCVARYDKIHLFRFDNGVERYDESRVLERGNTPVRFAMASRDGHRWQVGLSVCYD
                     LRFPELYRSYAQQGVDLILVPSAFTHTTGKAHWEVLLRARAIENLAYVAAAAQGGVHP
                     NGRHTWGQSIAIDPWGQVLAEQEQDPGVVLADLDAHMLVQRRTQLPVLEHRVLTEGTP
                     "
     gene            complement(4844..8905)
                     /locus_tag="PKX82_00170"
     CDS             complement(4844..8905)
                     /locus_tag="PKX82_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HOF50685.1"
                     /translation="MIPTPSLLLRAVASAARWALWLLALGWLTLGLVWGGLHFLIVPR
                     IGELRPWVEQQASSALGISVRIGSIVARSNGLIPSVEIQDVRLLDPQGREVLRLPQVL
                     AALSPHSALGLGFEQLYVEGLVLDIRRTADGRFWVAGMALPQTTDSDGAGADWVFSQT
                     ELAVRHGTVRWTDEMRGAPTLELTDVDVVLRNSGKSHALRMDATPPASWGARLSLSGI
                     FRQPLLSRSAGNWREWQGQAYAHFPHVDMAQLRRYLDLGVDVAQGAGALRAWVDVNRA
                     EFTHATADVAMDQVNVRVSPKLEPLMLTTVSGRVGARRFDDGYEFSTEALAFHTQDGL
                     HWPGGNVRVGLFSGDARKPAHGEISADRLDLAAMVEIAQRLPLDATVHQALAAYKPKG
                     LVEQMQATWLGAVATPERFTAKGRVVQLEIAAQSAPQNTPGVQGGTVDFDVTQAGGRA
                     TISVQGGAVDLPGIFEASVVPLDQLSGDVQWKLEGERIEVALPNLRFSNADVQGEAQV
                     KWQTAEVAKGVVNQQGRFPGILDLQGTLSRAQGAQVHRYLPLVLDESVRFYVRDAVRA
                     GVASNVKFKLKGDLRTFPYANAKQGDFRISADVKNVTYAFAPAFLLPKDSLPWPALTQ
                     LSGELVIDHDTLQIKSANGVLGSAGLQFFKTEGLVKNLYDGATVTVTGDLKGPLNDVL
                     TIVNGSPLGGLTGKVLSRATAAGAADYKLKLGFPIAAVDRITVQGAVVMAGNDVQISP
                     ETPKLTRLRGTLAFSESGFTVSGGQARALGGDVRIEGGLNTAAAKVGTPVKTPATTLR
                     IQGTATAEGLRQAKELGFAARLAQYATGASTYQATVGLRGGVPELLISSSLTGLALNL
                     PAPFAKSAEAALPLRIETSALRSPVLSGASQLDQLQFDVGRLANIAYVRDVSGADARV
                     MRGSIGVGLAADETAPMPDQGVVANVNLSTVDVDAWSQVLAKVSGSEIDTALAVGQPG
                     MAYLPTSLAVRARELLVGGRTLHNVVVGGSRDGQLWRANLDATELSGYVEYRQSTGPT
                     AGRVYARLARLVIGQSTAQDVENLLDEQPTSIPALDVVVEDMELRGKRLGRVEIDAVN
                     LAAGASVRDVAREWRLNRFNIITPEAVLTASGNWTHIAPVATTPSNRSIKERRRTALN
                     FKLDIADAGDVLSRFGMTGVVRRGKGKIEGQVAWMGSPITLDYPSLGGSFNVNVETGQ
                     FLKADPGLAKLLGVLSLQSLPRRLVLDFRDVFSEGFAFDFLRGDITIERGIARTNNLQ
                     MKGVNAAVLMEGQADIAKETQNLKVVVIPEINAGSASLIASAINPLVGLSTFLAQVIL
                     RRPLIEANTQEFLVDGTWVDPRVTRVERKTAPN"
     gene            8942..11650
                     /gene="glnE"
                     /locus_tag="PKX82_00175"
     CDS             8942..11650
                     /gene="glnE"
                     /locus_tag="PKX82_00175"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HOF50686.1"
                     /translation="MTRNGAAPLAQESRFVQRIRRRYAEQLSLLPSGAPTRAAMQAVF
                     DALRSTGADISQALRTTRQLVLERLTCLDCDADTPLELVTLAMTELAEWALDISFDEG
                     CKALDQSHGAPLAPNGERAQLCIVGMGKLGARELNVSSDIDLIYIYDHDGETAGNAEG
                     RSRISNQEYFSKLVRAMYALVGDTTEHGFVFRVDLALRPNGNSGPPAVSLGALEEYFH
                     VQGREWERFAWLKSRVVAPSSAVGSSCALYLRSLVVPFVFRRYLDYNVFDSLRVLHRQ
                     IRDHAARRSAGHPERNNDVKLSRGGIREIEFTVQLLQVVRGGQFPELRTRPTVSALQR
                     IATAGLMPQSTADALAQAYLFLRKVEHRIQYLDDQQTHVLPSGDNDLGWIAQTLGFAD
                     TCAFLCQLDAHRELVAQEFDALLGGAQHGCKGCGGKSSGDANDFDALLADLEGPFRDR
                     VASWREHPRVLALREESRARLGRLVQRTAQWLADGRVTEDAAVRLADWIEPLLRRESY
                     LALLLERPTVHERLLRMLGAARWPARYLLKHPGVIDELAGTAMFEERFDAQDFEQELE
                     RRRTALQGSGEDDDEALLNLLRRAHHAEVFRTLARDVEGKLTVEQVADDLSALAESVL
                     RTTAAWCWQRLKNKHRPDHRFAIIAYGKLGGKELGYGSDLDIVFVYDDEDERAGEVYA
                     ALVRKLINWLTVKTGEGDLYEIDTALRPNGNSGLLVTSFASYAAYQQQRGSNTAWTWE
                     HQAMTRARCVLGAPELQARFDAVREAVIKTERGEKGLRDEIGNMRTKVSASHAIKGEQ
                     FDVKHSPGGMVDIEFVVQFLVLSQGGRHPELLPNVGNIALLQRAQACGLLPGDVGDKA
                     AQAYRTLRQIQHRARLNEEPTQLDIAKVTTERDAGLALWTAVFGVH"
     gene            11657..12475
                     /locus_tag="PKX82_00180"
     CDS             11657..12475
                     /locus_tag="PKX82_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HOF50687.1"
                     /translation="MRIPSAAVLLLGAATTYAQAQDAGLPLWEVGMAGGALESPAYPG
                     STEKLRLAAALLFLIYRGEVLRIDRGGLDARLVHQDKFELDIGFSGSLPASSGDIAAR
                     TGMPDLGTLVEFGPRAKWTLLQHGTSRVQLVLPLRTVLELGGGFRGQGNAFEPALSYE
                     TRDGLSDWRMSARVSLVIADSKLGEYFYGVSQPYVTASRPYYEAKAGLLLSRLTLDAA
                     KNITPDLRVFGFVRYDSYAGAANASSPLMRQSYGTTVGVGFAWLLGRSAERVAW"
     gene            complement(12482..12976)
                     /locus_tag="PKX82_00185"
     CDS             complement(12482..12976)
                     /locus_tag="PKX82_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOF50688.1"
                     /translation="MQAHDAPPAFLDKIARREDLRPALDKLPKPWVFTNGVFDVLHRG
                     HVMYLAQARALGGSLIVALNTDASVRRLGKGDDRPLNNEMDRAIVMASQQAVGLVTWF
                     DEDTPVDIIGEIRPDILVKGGDYDMSKLPETRLVESWGGHALALPFVQGYSTTKLVQR
                     IRAG"
     gene            13071..14351
                     /locus_tag="PKX82_00190"
     CDS             13071..14351
                     /locus_tag="PKX82_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOF50689.1"
                     /translation="MNLVPVSFDSIRIGQPLPFPLVDEDGVLLARKSFVVESRAHLED
                     IVGRGRGLFIDVSDSGALHRAYVDQLQTLVRSEKSLGEIADTKLSAEALRKRVVDDER
                     IDWLDLQVQTNYLLRDSNPETFQSRLVRIDDILSEQTQRNPDGTLFALIHLSATETQL
                     YSATHAMLVSAMCSLASREVLGWSESIDAIVRRVALTMNVCMTDLQDKLALQVEPPTP
                     EQRAAIDNHPKGSGDLLRTLGVGDPLWLEAVRYHHHKLPGPLLDKTPAQRMARLVQRA
                     DMLDKTPAQRMARLVQRADMFAARLAPRASRVPISPAAAMQACYFDENRQVDEAGAAL
                     IKAVGIYQPGSFVRLATDEVGVVVRRGQNTSTPRVAVVVNRSGMPTGEPIVRDTSARE
                     HRIVASVPHREVKVKINLERMLPLTATTATDRPW"
     gene            14476..15780
                     /locus_tag="PKX82_00195"
     CDS             14476..15780
                     /locus_tag="PKX82_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF50690.1"
                     /translation="MKIAIIGSGISGLAVAHTLAGKADVTLFESGNYFGGHTHTVDVT
                     LPGPHGSVTHGVDTGFLVFNERTYPNLIALLGELGVATAKSDMSFSVQVPARKGHKAL
                     EWSGSSLNTVFAQRGNLLNPRFLRMLRDVLRFNRLATDIATRNAESELMQPLHMFLTQ
                     HGFSSEFRDWYFLPMLGCIWSCPTDQMLQFPVATMIRFCHNHGLIQVTNRPQWWTVSG
                     GARNYVDRIVAQVQDKRLNTPVERVERDAQGVLVTSRGQTERFDKVVMACHSDQSLAM
                     LSTPSPLERSVLGAIRYQPNRAVLHTDTSALPANKRAWAAWNYERSADTRQDAAQVCL
                     HYLLNMLQPLPFAQNVVVSLNPVRDIDPKQIHGSFEYAHPVFDLEAIQAQGQVGSLQG
                     QQHTYFCGAWTGYGFHEDGLKSGLHVARQLLALSAAPLEQQA"
     gene            15777..16550
                     /locus_tag="PKX82_00200"
     CDS             15777..16550
                     /locus_tag="PKX82_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HOF50691.1"
                     /translation="MTATAHQAMLGFGSVRHVRLRPVRNAFDYGTYFLMLPMRTLQRN
                     GNGTLARNRLAALSFFDKDHGDGRGPEQGGALAWLEALLHREGIDGADGEIWLHCYPR
                     VLGFTFKPVSFWYCHATDGSLRAIVVEVNNTFGERHCYLLDRPRYGTELQAAKVFHVS
                     PFCPVEGGYRFRFMVTPDLTRTVARIDYDDAAGPLIQTSVSGTLEPLNAASVRRALWR
                     YPAMTLGVVARIHWQALKLLVKKTPFFGKPTPPTTFTTR"
     gene            16576..17808
                     /locus_tag="PKX82_00205"
     CDS             16576..17808
                     /locus_tag="PKX82_00205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HOF50692.1"
                     /translation="MTTLHPSAVRSFALPRGTPGAARTVLKLLQRLRHGSLTVQLPDG
                     SMQRFGGDAAPHATLHLHNWKVFSAALKSGDIGFAESFIAGDWSTPNLTELLRVLVKN
                     RGEMEGVIYGSWLGRLFYRVRHLLHRNTHANSRKNIHAHYDLGNAFYTLWLDETMNYS
                     SAIFEGDTTQPMALAQKTKVRRALTQARVRPGDRVLEIGCGWGALAEMATAESGASIT
                     GVTLSTEQLEFARARMQRLGTSNQADLRLQDYRDIDDAPFDAICSIEMVEAVGSEYWP
                     TYFATVAKLLKPGGYACVQSIVIDDALFDRYLHSTDFIQQYIFPGGCLPCPREFRAQA
                     EAAGLEVVDAYAFGHDYAETLRRWREAFLARRDEVLAVGFDEKFMRIWEFYLAYCEAA
                     FDEDNIDVVQYTLRKRAD"
     gene            17811..18386
                     /locus_tag="PKX82_00210"
     CDS             17811..18386
                     /locus_tag="PKX82_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOF50693.1"
                     /translation="MAPLRIRRRTLLGAGAQALSLGLLGLHDASAQPEVPLEIRNGVS
                     SARLAGTARMRFFGLNIYDARLWVAPGFQASAFALHPLALELTYLRALNGRSIAERSL
                     KEMQRTAPLTTEVAQRWLGLMQDAFPDVVEGDRLTGLHRPGTGAQFWLNGQPRATIPE
                     AEFSRRFFGIWLSEDTSEPRLRTELLAGNTP"
     gene            18396..19697
                     /locus_tag="PKX82_00215"
     CDS             18396..19697
                     /locus_tag="PKX82_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF50694.1"
                     /translation="MRTPTTLPLPATPFTWRDGWRYGLMGFPLAFVALPLYVTLPNHY
                     ARHFGVPLATLGLVLLFARLFDAFVDPVLGRWGDRLYKQSPRAVLRWAALAAVLLGAG
                     FALLFFPPVQHPDALLVWAVGCLLLTYTAYSTLSILHQSWGALLGGDEAQRSRVVAWR
                     EGLGLAGVVMASITPVALGLSATVAIFFIALAVGWSAWRCARQPELTHPASEFSGIAV
                     RPESSLWHPFSRPAFRALLAVFVVNGIASAIPATLILFFVQDRLQAPSAVEPLFLGSY
                     FVCAALAMPLWLAVVRRLGLARTWGLGMLLSIAVFVFASTLGAGDTTLFVIVCALSGF
                     ALGTDLALPGAMLAGTIAASGDRGHAEGAYFGWWNFATKLNLALAAGLALPALGLLGY
                     TPGSRDPEALQMLTWAYCLLPCLLKAIAAVLLYTRILRKPL"
     gene            19694..20236
                     /locus_tag="PKX82_00220"
     CDS             19694..20236
                     /locus_tag="PKX82_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HOF50695.1"
                     /translation="MKRRLLLLSGTAATAMLVGGCASQQLDSYTAEKPVLDLQQYFNG
                     TLDAYGIFTDRSGTVVKRFTVVMRCTWQGNEGVLDEDFLYSDGSKQRRVWRITKLPGG
                     RYTGTADDVVGVAQGQAQGNALNWSYTLSLPVDGKVYEVQFDDWMYLMSDTVMLNKAT
                     MRKFGVRLGEVTLAFTRRAP"
     gene            20233..21012
                     /locus_tag="PKX82_00225"
     CDS             20233..21012
                     /locus_tag="PKX82_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF50696.1"
                     /translation="MSFFQPLNPPLKEWRGKTVWIVGASTGIGLATAEALHTLGARVI
                     VSARKAASLDAFVAHHPGSMALALDATDRQAVGQAARQLQALGPLDCVVYCAGHYNAM
                     RAWALDPDDMLRHMEVNYSGAIHVLDAVLPQLLARGEGHISLVGSVAGYRGLPNGLAY
                     GPTKAALINLAETLYLDLRDRGIGVSIINPGFVQTPLTAANQFAMPALLTPREAATAI
                     VKGWAEGAFEIHFPKRFTLWMQALRLLPNRLFFQAVRRATS"
     gene            21009..21467
                     /locus_tag="PKX82_00230"
     CDS             21009..21467
                     /locus_tag="PKX82_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOF50697.1"
                     /translation="MNNANHNPPAIPLHASRLVAFFESLTPASVAEMALFYDPQARFK
                     DPFNDVTGLPAIQHIFTHMFVALDSPRFVVTNQMTQGAQCFLTWEFRFAFRNFKKGQE
                     QVILGSTHLVFSNEGLVTLHRDYWDAAQELYEKLPVVGSLMRWLRKRANS"
     gene            complement(21464..21691)
                     /locus_tag="PKX82_00235"
     CDS             complement(21464..21691)
                     /locus_tag="PKX82_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50698.1"
                     /translation="MANYKTIYQCRECGATSYQRVIGRAENGALVPTGQYKCTGCRNV
                     FDTLRAWWEPRRATEFQANSLSTSEQHSTFA"
     gene            complement(21777..22391)
                     /locus_tag="PKX82_00240"
     CDS             complement(21777..22391)
                     /locus_tag="PKX82_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOF50699.1"
                     /translation="MKLLGSTSSPYVRKVRVVMAEKKLDYTFVPEDVWSADTTIAFSN
                     PLGKVPCLIMEGAEALFDSRVIVEYLDTLSPVGKLIPSVGRERAEVKTWEALADGLLD
                     ASILARLEATWGGRTEAQRSQAWIDRQMAKVNDAVKAMNQGLGDKPYCSGIHLSLADI
                     AVGCALGYLDFRFPQLDWRSEYPALVKLHDKLMQRPSFADTVPV"
     gene            22487..23866
                     /gene="purB"
                     /locus_tag="PKX82_00245"
     CDS             22487..23866
                     /gene="purB"
                     /locus_tag="PKX82_00245"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOF50700.1"
                     /translation="MTFSAISALSPLDGRYAAKLSTLRPLTSELGYMHRRVQVEVAWF
                     IALSDAGFAEFKPLSPGARTYLLGLVKNFSEADGEAIKAIEKTTNHDVKAVEYWIKSK
                     FEARPELEHASEFVHFACTSEDINNTSHALQLRSSRDVVVLPALDKIILKLRDMAHAY
                     ADVPMLSRTHGQTASPTTVGKEIANVVVRLQTACERIANIKMLAKMNGAVGNYNAHLS
                     AWPDFDWEAFSKKVIETPEPLGLGLTFQPYSIQIEPHDYMAELFDAVARTNTILIDLS
                     RDIWGYVSLGYFKQKLKDGEVGSSTMPHKVNPIDFENAEGNLGLANAVLRHLSEKLPI
                     SRWQRDLTDSTVLRNIGVALGYAVLAYSSLMTGLNKLEINEAAIAEDLDSSWEVLAEP
                     IQTVMRRFGLPQPYEQLKKFTRGAAMTRELMQGFIAGLDLPQSEKDRLLAMTPGSYTG
                     KASELARRV"
     gene            23866..>24045
                     /locus_tag="PKX82_00250"
     CDS             23866..>24045
                     /locus_tag="PKX82_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HOF50701.1"
                     /translation="MALKSTIYKANLQIADIDHSYYADHALTLARHPSETDERMMVRL
                     VALALNAYKLQSECNG"
BASE COUNT         4583 a         7736 c         7163 g         4563 t
ORIGIN      
        1 gtggtcgtcc cccgccaccg cgatcacgcc gccccagggc gtggtgccgg ccatgttggc
       61 gtgtttgaac acatcggagc aacggtccac gcccgggccc ttgccgtacc agataccaaa
      121 tacaccgtca tatttgttgc tgccgggtgg tgcaaaaccc aactgctggg tgccccagag
      181 cgctgtggcg gccagctcct cgtttacccc cggctggaac acgacatgat gggccttaag
      241 gtatttctcg gccttttgca gagactggtc ataggtgccc agcggcgagc cgcggtagcc
      301 actgataaag cctgccgtgt tcttacccac acgcatgtcg cgcacacgct gcagcatggg
      361 cagccggacc agggcttgca ccccgctcat gaaggcctgg ccatattcca gggaatattt
      421 gtcgtcgagg gtgaccgttt ccagtgcctt gcggatgtgc tcgggcaggt cgcggctcag
      481 gggtgcattc atggtgcatg tctccgatat agttcgcgca agtgtattcc ggcaccgctg
      541 atatgtcttt gcgttctttg ctctaaaagc gccacacatg gaaagaatct ttctccataa
      601 tgccatgcat ggaaacactt gaccgatttg acatcgccat cctgaatgaa ttgcaaaacg
      661 atggccgact gagcaatgcc gaactggcct cgcgcgtggg gctgagcgct gccccgtgct
      721 ggcgccgggt caaggcgctg gaagagagcg gcttcatcac cggctaccgt gccgagatca
      781 accgccacaa aaccggcctg ggcgtgctgg ctttcgttcg gctggacgcc gaccgcaaca
      841 gcggcgatgt gctgcaggcc ctggaaaacg ccatccgcac catccctgaa gtcatcagct
      901 gccactacat cagcggtaac ggcacctttg agctgcaggt ggtcagtcgc gacctcaaca
      961 ccttcagcca gttcgcgcgc gaggtactga tcaacctgcc caacgtgaaa gacctgcaca
     1021 ccagcttctc gctcggcgaa gtcaaggcca gcagtgccct gccgctgggc cacctctcgc
     1081 ctgcctcctc tgcagccacc accacacggg gtcgttcatg aaaaagcgcc tgctacttgt
     1141 ttttatgtgt ttttggcctc tagcccccgt atctattgcg cagttagcta ctaaatcgat
     1201 agcaaacccc aacccccttc cgctgcgtaa tctgctcatc gaggtgcgac aggtgcagtc
     1261 tggcagtgcc cagcaatccg gcatggaaac cggtggtgct gcgcgaatcg gcaacgacgg
     1321 gcaactctcg atgcagggac agttgcaggc acgccagcag cagtcgcaac aaggtgcaac
     1381 ggcaatccaa caggtgatgg tgctcaatgg ccgtaacgcc catattgcgt tgcgcacagc
     1441 ccagcccctg cgcctgctgc agtcgttcgt acgcaatggc cgggtggtcg tcacgcagag
     1501 cgtggtgttt ctggaagcgg gtaccggctt cctagccacc ccgcgctggg acggcgctga
     1561 actggtggaa ctatcgattg ctgcccagca ggcactcccg aatgcaagtc gcataactgg
     1621 cgcaacctcc acggcgagca cagaaagcgt gctgctggtg ccactgggtc agtggacgcc
     1681 cattgcgcag tcggaccttt ctgccagcaa tgacacccag agcctgggag gcaacagcgc
     1741 atcggccatg caatccagca ccgaagtgca ggtacgcatc acggtgcgct gacgcacaac
     1801 cgcgtcagcg gttcagcacc gagcgaatcg tggcgcccag ctcttcggcc acaaacttgg
     1861 ccacgtaggc atcggccccc acgcttttga catgcccttc gttggtggta ccggtcaggg
     1921 aggagtggat gatcaccggg atgccggcaa agcgcggatc ctgcttgatg ttgcgggtca
     1981 acgtgaaacc atccatttca ggcatttcca ggtcggtcag caccagcgcg accttgtcgc
     2041 ggatggactt gccagcggct gtggcttcga cctgcatggc ctgcagtttg tcccaggctt
     2101 ccttgccggt cttggtcatg acgtactgca cattcatggc ttgcaggcct tgctcgatca
     2161 tcatgcgggc cacggccgag tcatccgcgg ccaggatgac ggagccgggt ggaatcgaca
     2221 ctttcggcat gacagcagat tcatcgaggg acttgtgctg ctcggggaac acatcgcgca
     2281 ggatctgctc cacgtccagc acctgggcca aacgggtgtt ctccgcattg ccatcgagcc
     2341 gggcaatgct ggtcaccagc ccgccgcccg taccttcggc ggaaagcacc tgtttccact
     2401 ccagacggac aatttcattg acctcttcca ccgcaaacgc ctgcgtggtg cgcgcaaact
     2461 cggtcaccag cagaatggcc agccccttgg tgggattaca gccggtaatc agcggcaggt
     2521 tgaccaccgg aatcatctga ccccggatat ttgccacacc catgaccgac ggaggtgcat
     2581 tcaccatggc agtgatttcc ggcatgacca tgatctcgcg cactttgaac acattgatgc
     2641 caaacagctc gcgcttgtgg gtggctggcg actcgcccaa acgaaacagc agcaactcaa
     2701 acatgctgtt gctcgccaga ttggtccgct catcaatgtc gcgcattact gatgtcttgc
     2761 tcatggtgtc atcctgtcgt tatgagtgaa ttgctggtcg cacagatgtc atggtaacga
     2821 cttcccggct actttggcca gaacgtccac aactgcagcg gctggcgcaa ccgtccggcg
     2881 agatcggccg cttccgcacc ccaaccagca aagtagtctg cccgtatcgc accggtaatg
     2941 gcggtgcccg tatcctgcgc cagtaccagc ctctggaggc tcacctgggg gccgctggat
     3001 accagccaga cgggtgtgcc gtagggaata cttccggggt ccaccgcaat ggagcgtccc
     3061 ggcgtcagcg ccacgccctg cgcgcctttg ggtccaaagg cagcatccag atcggacaac
     3121 ggctcttcct tgaaaaaaac catgcgcggg ttactccaca gcaactcctg acgccgctgt
     3181 gggttttgcg ccagccaggt cttgataccg ggccacgagg cgtccctcac cagcccttgg
     3241 tcgagcagcc agcgccccac actgcgatag ggctggtcgt tggtgcccgc ataggccaca
     3301 cgaatccagc gctggctacc gtcaggttgg gcaattcgca accggccgga tccctgaatc
     3361 tgcaacacca gtgcatcgat cgggtcggcc aggtacgcaa tcgcccggcc ctgcaacgca
     3421 gcctgcgctt ccggtaaggt gtcgatttcc tggcgggtgt accaaggctt gcgctgcccc
     3481 agactcaacg gcggctggta cagcggaacg gtaaaagtgg ccgtgggcac gcggctggct
     3541 tccagctgcg gctcgaaata cccggtcagc agcccggtag attcgccctg cgcggattcc
     3601 acccggtagg gctgaagccg cgcctgcatc cagtcgcgtt gcgcctgggc gtcggcaatg
     3661 ctcaggcgcc gcacctcgcc acacagcgct cccatcgggg ctacaggctt ctcgcaactc
     3721 ttgagccagg cgttccaggc ttcaaacagg ttgtcctgcc caaagcccgg cagttcggac
     3781 caggcaacag gcacccatcg gctgcgcggc tgggtcaggg aaccccctgg acccgaagga
     3841 ataggagcag gcacaacgac atgccgcgcc actggggcgg gcggcgcggt gggatacacc
     3901 atgggggcac tgccgcagcc agccagtatg ccagccacag caaccacagc ggccacactg
     3961 caccccaccc acagccggcg gttgaaagag tcacgcatca tggtgtgccc tcggtcagca
     4021 cccgatgctc cagcacaggc aactgcgtgc gccgctgcac cagcatgtgt gcatccaggt
     4081 cagccagcac cactccggga tcctgttcct gttcggccag cacttgtccc catgggtcga
     4141 tggcgatgga ctgtccccag gtatgccgcc cgttcggatg tacgccaccc tgtgctgccg
     4201 ccgccacata cgccaggttt tcgatagcgc gcgcgcgcaa cagcacctcc caatgcgcct
     4261 tgcccgtggt gtgggtgaag gcgctgggca ccagaatcag gtccacaccc tgctgcgcat
     4321 aactgcggta caactccgga aaacgcaggt cataacagac gctcaggccc acctgccagc
     4381 ggtgcccgtc ccgcgacgcc attgcaaacc gcacgggggt gtttccgcgc tccagcacgc
     4441 gcgactcgtc gtagcgctcc acaccgttgt caaagcggaa caggtggatc ttgtcgtaac
     4501 gtgccacgca aaggccctgg ggattgaaca ccagcgtgct gttgaacacc cggtccgcct
     4561 gcgttgccgt cagcggcagc gtcccaccca cgatccacag tttcaaacga cgcgccgtgt
     4621 cggccagaaa ctgctggatc gggcccttcc catagggctc ctggatggcc agcttgtccg
     4681 catcgttctg gccaatgaga caaaaatact ctggaagcac agccagttcc gcgccctgcg
     4741 cggccgcctg atcgagcagg gcagcggcct gctgcaggtt gtcgttcagg tcgccggtag
     4801 acaccatctg aatggctgca attttcatgt ggactccata cgcttagttg ggtgccgtct
     4861 ttcgctccac gcgggttacg cgtgggtcca cccaggtgcc gtcgaccagg aactcctggg
     4921 tgttcgcctc gatcagcggc cgacgcaaga tcacctgcgc cagaaaagtg ctcaacccta
     4981 ccaggggatt gatggcagac gcgatcagtg aagcacttcc tgcattgatc tcgggtatca
     5041 ccaccacctt gaggttctgg gtctccttgg caatgtccgc ctggccttcc atcagcaccg
     5101 ccgcattgac acctttcatc tgcaggttgt tggtgcgcgc gatgccccgc tcaatggtga
     5161 tgtccccgcg cagaaaatca aaggcaaaac cctcactgaa gacatcgcga aaatccagca
     5221 ccaaccggcg tggcagagac tgcaggctca gtacccccag cagcttcgcc aggccagggt
     5281 ctgccttcag gaactgcccg gtttccacgt tcacattgaa acttccgccc aggctggggt
     5341 agtccagcgt aatcggggag cccatccagg ccacctggcc ttcgatcttg cccttgcccc
     5401 tgcgcaccac accggtcatg ccaaagcggc tgagcacatc gcccgcatcg gcaatatcga
     5461 gtttgaaatt gagcgccgtg cggcggcgct ccttgatgct gcgattcgat ggcgtggtgg
     5521 ccaccggagc gatgtgggtc cagttgccac tcgcggtgag cactgcttcg ggtgtgatga
     5581 tgttgaagcg gttgagtcgc cattcgcgcg ccacatcccg gacggacgca ccagccgcca
     5641 gattgacggc gtcaatttcc acgcgcccca gcctcttgcc acgcaactcc atgtcttcca
     5701 ccacgacatc cagcgctgga atgctggtgg gctgctcgtc gagtagattc tccacatcct
     5761 gtgccgtgct ttgaccaatt accagccggg ccagccgggc gtacacgcgg ccggccgtag
     5821 gccctgtgga ctgccggtat tccacatacc cgctgagctc tgttgcatcc agattggcgc
     5881 gccacagctg tccatcacgc gatccgccca caacgacgtt gtggagggta cgcccgccga
     5941 ccagcaactc gcgcgcgcgc accgccagac tggtcggcag atacgccata ccaggctgcc
     6001 ccactgcgag cgcagtgtcg atttcggatc cggacacctt ggccagcacc tgcgaccagg
     6061 catctacatc cacggtcgac aggttgacgt tcgccaccac cccctggtcc ggcatgggtg
     6121 cggtctcatc cgcggccaga cccacaccga tggaaccccg catcacacgg gcatcggccc
     6181 cagacacatc ccgcacatag gcaatattgg ccagtcgccc cacgtcgaac tgcaactggt
     6241 ccaactggct ggcaccggac agaacggggg accgcagcgc tgaggtttct atgcgcaatg
     6301 gcaaagctgc ttcggcagat ttggcaaaag gagcgggcag gttcaacgcc agtccggtaa
     6361 gcgaactgct gatcagcaac tcaggcaccc cgccacgcaa ccccaccgtt gcctggtagg
     6421 tggacgcgcc tgttgcgtat tgcgccaggc gcgcggcaaa gcccagctcc ttggcctggc
     6481 gcaatccctc ggcagtggcc gtgccctgga tccgcaacgt cgtcgccggc gtcttgaccg
     6541 gggtaccgac cttggccgca gcggtattga gcccgccctc gatgcgcaca tcaccgccca
     6601 aggcgcgtgc ctgtccaccc gataccgtaa agccactttc ggaaaacgcc agcgtgccac
     6661 gcaagcgcgt gagcttgggt gtttccggag aaatctggac atcgttgccc gccatcacca
     6721 ccgccccctg caccgtgatc cggtccaccg cagcgatagg aaagccgagc ttgagcttgt
     6781 agtctgccgc cccggcggcc gtggcccggg acaacacctt gccggtcaaa ccacccaacg
     6841 gcgaaccatt gactatggtg agcacatcat tgagcggccc ctttaaatct ccggtcacgg
     6901 tgacggttgc cccgtcgtac aggttcttga cgagcccttc cgtcttgaag aactgcaagc
     6961 cggcgctgcc taggacgccg tttgcgctct tgatttgcag cgtgtcatgg tcaatcacca
     7021 actcgcccga caactgcgtc agtgcgggcc agggcaggct gtctttgggc aacaggaacg
     7081 ccggtgcgaa ggcgtaggtg acatttttca catccgccga gatgcgaaag tccccctgtt
     7141 tggcattggc gtaaggaaag gtacgcaaat cccccttgag cttgaacttg acattcgatg
     7201 caactcctgc ccgcacggcg tcccgcacgt aaaagcgcac cgactcatcc agcaccagcg
     7261 gcaggtaacg gtgcacttgc gcgccctgtg cacggctcag ggttccctgc aagtccagaa
     7321 ttccggggaa gcgcccctgc tggttcacca caccctttgc gacctccgcg gtctgccact
     7381 tcacctgggc ttcgccttgc acatcggcat tgctgaaacg caggttgggc aacgcaacct
     7441 cgatgcgttc gccttccagt ttccattgca catccccgct cagctggtcc aatggaacca
     7501 ccgacgcttc aaaaatgcca ggcagatcca ctgccccacc ctgcacggaa atggtggcgc
     7561 gccctcctgc ctgggtcaca tcgaaatcta ccgtgccccc ctgcacgcca ggtgtgttct
     7621 gcggggcgct ttgcgcggca atttccagtt ggaccacacg gcccttggca gtaaaacgct
     7681 cgggcgtggc caccgcgccc agccaggtgg cctgcatctg ctccaccagc cccttgggct
     7741 tgtaggctgc cagcgcctgg tgtacggttg cgtccaacgg caagcgctgg gcaatttcca
     7801 ccatagccgc caggtccagc cggtccgcac tgatttcccc atgcgcaggc ttgcgggcat
     7861 cgccgctgaa caggcccacc cgcacgttgc cgcctggcca gtgcaaacca tcctgcgtat
     7921 ggaaagccag cgcttcggta gagaactcgt agccatcgtc gaaccgcctg gcacccaccc
     7981 ggcccgacac agtggtcagc atcagcggct ccagcttggg cgatacccgg acgttgacct
     8041 ggtccatggc cacgtccgcg gtggcatggg tgaattcggc ccggttgaca tccacccagg
     8101 cgcgcaaggc gccagcgccc tgcgcgacat ccacacccag atccagataa cgacgcagct
     8161 gcgccatgtc cacatgcgga aagtgggcgt acgcttgtcc ctgccactcg cgccagttac
     8221 ccgcactgcg gctcaacagc ggctggcgaa agatcccaga caggctcaac cgagcgcccc
     8281 aggaggcggg tggcgtggca tccatacgca atgcgtggga tttgccagag ttgcgcagca
     8341 ccacatcgac atcggtcaac tccagcgtcg gcgcgccacg catctcgtcg gtccaccgca
     8401 ccgtgccatg gcgtaccgcc agttccgttt gggaaaacac ccagtccgca cccgccccat
     8461 cgctgtccgt ggtctgtggc agggccatgc ccgccaccca aaaccggcca tcggccgtac
     8521 ggcgaatatc cagtaccagg ccttccacat acaactgctc gaaacccagt cccagtgccg
     8581 agtgtggcga cagcgctgcc agcacctgcg gcagacgcaa tacctcccgc ccctgcgggt
     8641 ccagcaagcg aacatcctgg atctcgaccg acgggatcaa cccgttggaa cgggccacga
     8701 tggagccgat gcgtaccgag attcccaacg cactggaggc ctgttgctcg acccaaggac
     8761 gcaattcacc gattcggggc acaatcagaa aatgcagtcc accccagacc aggcccagcg
     8821 tgagccagcc taaagcgagc agccacaagg cccagcgcgc agccgaggca acggctctga
     8881 gcagcagcga tggggttggt atcaaatcaa atctcgtgca ttggtagcta tgtcaggaat
     8941 tatgacccgc aacggggcag cgccactggc gcaggaatca cgtttcgtcc agcgcattcg
     9001 ccgcaggtat gcagaacagc tctccctttt gccctccggc gctcccacac gcgcagccat
     9061 gcaggccgtc tttgacgcat tgcgcagcac aggtgctgac atttcgcagg ccttgcgcac
     9121 cacacgtcag ctggtgctgg agcgcctgac ctgtctggac tgcgacgccg acacaccgct
     9181 ggaactggtg accctggcca tgactgaact ggcagagtgg gcacttgaca tcagctttga
     9241 cgaaggctgc aaggccctgg accagagcca cggcgccccc cttgccccca atggcgaacg
     9301 ggcgcaactg tgcattgtgg gcatgggcaa gctgggcgcc cgggagctca atgtctccag
     9361 cgacatcgac ctgatctaca tctacgacca cgatggcgaa accgccggca acgccgaagg
     9421 ccgcagccgc atctccaacc aggaatactt cagcaagctg gtgcgtgcca tgtacgcact
     9481 ggtaggcgac accacagagc acggcttcgt cttccgcgtc gatctggccc tgcgccccaa
     9541 cggcaactcc ggaccaccgg ctgtttcgct aggtgccctg gaagagtatt tccatgtgca
     9601 ggggcgcgaa tgggagcgtt ttgcctggct caaaagccgg gtagtcgccc ccagcagcgc
     9661 agtgggttcg agttgcgcgc tgtatttgcg cagcctggtc gttccgttcg tattccgccg
     9721 ctatctggac tacaacgtgt ttgactcgtt gcgggtgctg caccgccaga ttcgcgacca
     9781 tgcggcgcgc cgcagtgcgg gacatccgga gcgcaacaac gacgtcaagc tgtcccgcgg
     9841 cggcatccgc gagattgagt tcaccgtgca actgctgcag gtggtgcgtg gtggccagtt
     9901 cccagagctg cgcacgcgcc ccacggtcag cgccctgcaa cgcatcgcca ctgccggact
     9961 gatgccacaa agcaccgcgg acgcactggc gcaggcctac ctgtttctgc gcaaggtgga
    10021 gcaccgcatc cagtacctcg acgaccagca aacccatgtg ctgcccagtg gcgacaacga
    10081 cctcggttgg attgcacaga cactgggttt tgccgatacc tgtgcgttcc tgtgccagct
    10141 cgatgcccac cgggaactgg tggcgcagga gttcgacgct ttactggggg gagcgcagca
    10201 cggctgcaag ggatgtggtg gcaaatcgtc cggtgatgcc aacgactttg acgccttgct
    10261 ggcggatctg gaaggtccgt ttcgcgaccg tgtggccagc tggcgcgagc acccgcgcgt
    10321 gctcgcgttg cgcgaggaat cccgcgcgcg cctgggccgg ctggtgcagc gcacggcgca
    10381 gtggctcgcc gatgggcggg tcactgaaga cgcggctgtg cgactggccg actggatcga
    10441 gccgctgctg cgccgcgaaa gctacctggc gttgctgctg gagcgcccca cggtgcacga
    10501 gcgcctgctg cgcatgctgg gagcggcgcg ctggccagca cgctacctgc ttaagcaccc
    10561 cggggtcatt gacgagctgg ctggcaccgc catgtttgag gagcgctttg atgcgcagga
    10621 ctttgaacag gaactggagc gccgccgtac tgccctgcag ggcagcggcg aagatgacga
    10681 cgaagccctg ctcaacctgc tgcgccgcgc acaccatgca gaagtcttcc gcacgctggc
    10741 gcgcgacgtg gaaggcaagc ttacggtcga acaggtagcc gatgacctca gcgccctggc
    10801 ggagagcgtg ttgcgcacca ctgccgcctg gtgctggcag cgtctcaaga acaagcaccg
    10861 ccccgaccac cgctttgcca tcattgccta cggcaagctc ggcggcaagg aactgggcta
    10921 cggcagtgac ctcgacatcg tgtttgtcta cgacgacgag gacgagcgcg ctggtgaggt
    10981 gtatgccgcg ctggtgcgca aactgatcaa ctggctcact gtcaagaccg gcgagggaga
    11041 tctgtacgaa atcgataccg cactgcgccc caatggcaac tcggggctgc tggtgaccag
    11101 ctttgcctcg tacgcggcct accagcagca gcgcggcagc aacaccgcct ggacctggga
    11161 acaccaggcc atgacgcgcg cgcgctgtgt actgggcgca cccgaactac aggcgcgttt
    11221 tgatgcggtg cgcgaagcgg tgatcaagac cgagcgcggg gaaaaaggcc tgcgcgacga
    11281 gattggcaac atgcgcacca aggtcagcgc cagtcatgcc atcaagggcg agcagtttga
    11341 tgtgaagcac agcccgggtg gcatggtgga catcgaattt gtcgtgcagt ttctggtcct
    11401 ttcgcaaggt ggacggcacc ccgaactgct gcccaacgtg ggcaacattg ccctgctgca
    11461 acgggcgcag gcctgtggcc tgctacccgg cgatgtgggc gacaaggcag cgcaggccta
    11521 ccgcacactg cggcagatcc agcaccgcgc acggctcaac gaagagccca cgcaactcga
    11581 cattgcgaaa gtgactaccg aacgcgacgc cggcctggcg ttatggacag cggtgtttgg
    11641 ggtgcactaa acacctatgc gcatcccttc cgctgctgtg ctgctgctag gagcagccac
    11701 cacttatgcg caggcacagg atgctggctt gccgctttgg gaagtaggta tggccggtgg
    11761 cgctttggag tctcctgcct acccgggatc cacagaaaag ctgaggcttg ccgctgcgct
    11821 gcttttcctg atctaccgcg gagaggtgct gcgcatcgat cggggcggac tggatgcgcg
    11881 actggtgcac caggataagt ttgagctgga cataggtttt tccggctcat tgccggccag
    11941 ttccggggac attgctgcgc gcacaggcat gcccgatctg ggcacgttgg tggagtttgg
    12001 cccacgtgcg aaatggacct tgttgcaaca cggtaccagc cgtgtgcaac tggtgctgcc
    12061 tttgcgcacg gtcctggaac tgggcggcgg attccgaggg cagggaaacg cttttgaacc
    12121 cgccctgagt tacgaaaccc gggacgggct atccgattgg cggatgtcgg cacgggtcag
    12181 tctggtgatt gccgacagca agctgggcga atatttctac ggggtttccc agccttacgt
    12241 cacggccagc cggccgtact atgaggccaa ggccgggctg ttgctgtcgc gactgacgct
    12301 ggatgccgcc aaaaatatca cacccgatct gcgcgtattt ggcttcgtcc gctacgactc
    12361 gtacgcgggg gctgcgaacg ccagcagtcc gttgatgcgg caatcgtatg gaaccactgt
    12421 gggagtgggg tttgcctggc tgctgggccg atctgcggag agggtggcct ggtagaacag
    12481 gctacccggc ccggattctt tgcaccagct tggtggtgga atacccttgg acaaacggca
    12541 gcgccagcgc atggccaccc cagctttcca ccagccgggt ttccggcagt ttggacatgt
    12601 cgtagtcacc gcccttgacc aggatgtccg ggcggatctc gccaatgatg tccactggcg
    12661 tatcttcatc gaaccaggtc accaggccca ccgcttgttg gctggccatg acgatggcgc
    12721 ggtccatttc attgttcagc gggcggtcat cgcccttgcc cagccggcgc accgatgcat
    12781 ccgtattcag ggccacgatg aggctgccgc cgagcgcgcg tgcctgagcc aggtacatga
    12841 cgtggccgcg gtgcagcaca tcgaacaccc cattggtgaa cacccagggt ttgggcaatt
    12901 tatccaaggc agggcgcagg tcttcgcgcc gggcgatctt gtccaggaag gcggggggag
    12961 catcatgggc ttgcatgccc ccgatgctag cagggccaga ctgcacggtg cttgcccctc
    13021 aattccggct ttcagcgacc gatctgccat gcataatatt ttggtaactt atgaaccttg
    13081 tccctgtcag ttttgactcc atccgcattg ggcagccact tcctttcccg ctcgtggacg
    13141 aggacggggt cctgctggcc cgaaagtcgt ttgtagtcga atcccgcgcc catctggagg
    13201 acatcgtggg gcgcggcagg ggcctgttca tcgacgtttc cgattctgga gccctgcacc
    13261 gcgcttatgt agaccagctg caaacgctgg tgcgcagcga aaaatcactg ggcgaaatcg
    13321 cagacaccaa actctcggcc gaggcactgc gcaagcgcgt ggtggacgac gagcgcattg
    13381 actggctgga cctgcaggtg cagacgaatt acctgctgcg ggactccaat cccgagactt
    13441 ttcagagtcg cctggtacgc attgacgaca tattgagcga gcaaacccag cgcaatccgg
    13501 acggcacgct gtttgcactg atccacctgt ctgccacgga gacccagctc tacagcgcta
    13561 cccacgccat gctggtcagc gcgatgtgca gcctggcatc ccgcgaggtt ctggggtggt
    13621 ccgagtcgat tgacgccatt gtgcgcagag tcgcgctgac catgaatgtg tgcatgaccg
    13681 atctgcagga caaactggcc ctgcaggtgg agcctcccac ccctgagcag agggcggcga
    13741 tcgacaacca tcccaagggc tccggcgacc tgttacgcac cctcggtgtc ggcgatccgc
    13801 tctggctcga agccgtgcgc taccaccacc acaagctgcc aggcccgttg cttgacaaga
    13861 cgccggccca acgcatggca cggctggtgc agcgtgctga catgcttgac aagacgccgg
    13921 cccaacgcat ggcacggctg gtgcagcgtg ctgacatgtt tgcagcgcgt ctcgcgcccc
    13981 gcgcgtctcg cgtcccgatt tcacccgcag cagccatgca ggcctgctac tttgacgaga
    14041 atcgccaggt agacgaggct ggcgccgcgc tcatcaaggc cgtgggcatt taccagccag
    14101 gttcttttgt gcgcctggcg acagacgaag tgggcgtggt ggtgcggcgc ggacagaaca
    14161 cttcgacacc gcgcgtggcc gtcgttgtca accgcagcgg aatgcccacc ggggagccca
    14221 tcgtgcggga tacctccgcg cgcgagcacc gcatcgtggc cagcgtgccg caccgggagg
    14281 tgaaagtcaa aatcaacctc gagcgcatgc tccctctgac ggctaccacc gccacagacc
    14341 gtccctggta gtccctgcca acgctgcgaa tccatccgat ggagccaata aaccatgcta
    14401 attaaatggt tttaaaacct attgatgcga taaattacca atcagtcaca aaatagccga
    14461 aaggtttatt ttgttatgaa aattgcaatc atcggttcag gcatttcagg ccttgcagtg
    14521 gcccacacgt tggcgggcaa agccgacgtc actttgtttg aatcgggtaa ttactttggc
    14581 ggacacacgc acacagtgga cgtcaccctg ccgggtccgc acggctcggt gacccacggc
    14641 gtagacaccg gttttctggt gttcaacgaa cgaacctatc ccaacctgat tgccctgctc
    14701 ggcgaactcg gtgtggctac tgccaaatcg gacatgtcgt tctcggtgca ggttccggcc
    14761 aggaagggcc acaaggcact ggagtggagc ggctcatcac tgaacactgt cttcgcgcaa
    14821 cgcggcaatc tgctcaaccc gcgcttcctg cgcatgctgc gcgatgtgtt gcgcttcaac
    14881 agactggcca ccgacatcgc cacgcgcaat gccgaatccg aactgatgca gccgctgcac
    14941 atgtttctga cacagcacgg gttttcgtcc gaatttcgag actggtattt cctgcccatg
    15001 ctgggctgca tctggagctg cccgacagac cagatgctgc agtttccggt ggccaccatg
    15061 atccggttct gccacaacca tggcctgatt caggtcacca accggcccca gtggtggacc
    15121 gtgagcggcg gggcacgcaa ctacgtggat cgcatcgtgg cgcaggtgca agacaagcgc
    15181 ctcaacactc cggtggagcg ggtggagcgc gacgcgcagg gcgtgctggt gacctcgcgc
    15241 ggacagacgg agcgctttga caaggtggtg atggcctgcc actccgacca gtcgctagcc
    15301 atgttgagca cccccagtcc cctggagagg tctgtacttg gcgccattcg ctaccaacct
    15361 aatcgcgcgg tactgcacac cgatacctcg gccctgccgg ccaacaagcg cgcgtgggcc
    15421 gcctggaact acgagcgcag tgcggatacc cggcaagacg cggcccaggt gtgcctgcac
    15481 tacctgctca atatgctgca accactgccg tttgcgcaaa atgtggtcgt gtcgctgaat
    15541 ccggtgcggg acatcgaccc caagcaaatc cacggcagct ttgaatacgc ccacccggtg
    15601 tttgacctgg aagccatcca ggcacaaggc caggtgggca gtctgcaagg gcaacagcac
    15661 acctatttct gtggagcctg gaccgggtat ggcttccatg aagatggcct caagtcgggc
    15721 ctgcatgtgg ccaggcaact gctggcgctg tctgcggctc cactggagca gcaggcatga
    15781 cagccactgc acaccaggcc atgctgggct ttggctcggt acgccatgtg cgtctcagac
    15841 cggtgcgcaa tgccttcgac tacggcacct atttcctgat gctgccaatg cgtactcttc
    15901 agcgcaatgg caacggtacg ctggcccgta accgccttgc cgcactgagt ttctttgata
    15961 aagaccatgg tgatggccgc ggtccggagc agggcggtgc gctggcctgg ttggaggcac
    16021 tgctgcaccg cgagggcatt gacggggccg atggagagat ctggctgcac tgctatccgc
    16081 gcgtgctggg gtttaccttc aagcccgtga gcttctggta ttgccatgca acagacggca
    16141 gcctgcgcgc cattgtggtg gaagtgaata acacttttgg cgagcggcac tgctacctgc
    16201 tggaccgccc gcgctatggc accgagttgc aggcagccaa agtgtttcat gtgtcgccgt
    16261 tctgccctgt ggaaggtggc taccggtttc gcttcatggt gacgcccgac ctcactcgca
    16321 ccgtggcacg catcgactat gacgacgcag caggcccgct gatccagacc agcgtgagcg
    16381 gaactcttga gccactgaat gccgccagcg tgcgccgcgc cctgtggcgc taccctgcca
    16441 tgacgcttgg ggtggttgca cgcatccact ggcaggcact caaactgctg gtcaaaaaaa
    16501 cgcctttctt tggcaaaccg accccaccca ccacgttcac cacccgataa ctcccagaac
    16561 gagcgcgaga ccaccatgac gactctccac ccttccgcag tcagaagctt tgcgttaccc
    16621 cgtggcacac cgggcgctgc gcgcaccgtg ctgaaactcc tgcaacgcct gcggcacggc
    16681 agcctgacag ttcagctgcc cgacgggtcc atgcaacgct ttggcggtga cgccgcaccc
    16741 cacgcaacgc tgcacctgca caactggaaa gtgttttctg ccgcactcaa atccggcgac
    16801 attggttttg ccgaaagctt tattgcgggc gactggagca cgcctaacct gaccgagctg
    16861 ctgcgggtgc tggtgaagaa ccggggcgag atggaaggag tgatttatgg cagctggctg
    16921 ggacgcctgt tctaccgcgt gcggcatctg ctacaccgca acacccacgc caatagccgc
    16981 aagaacatcc atgcccacta cgacctgggc aatgctttct acacgctctg gctcgatgag
    17041 accatgaact actcgtcggc cattttcgag ggcgacacca cccaacccat ggcgctggcc
    17101 cagaagacca aggtacgccg agcactgacg caggcacggg ttcgccccgg cgaccgggtg
    17161 ctggagattg gttgcggctg gggcgccctg gcagaaatgg ccacggccga gtcaggcgca
    17221 tccatcaccg gagtgacgct gagtaccgag cagctggagt ttgcacgggc ccgcatgcag
    17281 cgcctgggaa ccagcaatca ggcagatttg cgcctgcagg actaccgcga cattgacgat
    17341 gcaccttttg atgctatctg ctccatcgag atggtggaag ccgtcggcag cgaatactgg
    17401 cccacctact ttgccacggt ggccaaactg ctcaaacccg gtggctacgc ctgcgtgcag
    17461 agcatcgtga tcgacgacgc cctgttcgac cgctacctgc actccaccga cttcattcag
    17521 caatacatct tccccggggg atgcctgccg tgcccgcgcg aattccgcgc ccaggcggag
    17581 gcagccggac tggaggtggt ggatgcctac gcgtttggcc atgactatgc cgagacattg
    17641 cggcgctggc gcgaagcctt cctggcccgg cgcgacgagg tgctggccgt gggtttcgat
    17701 gagaaattca tgcgcatctg ggagttctat ctggcgtact gcgaagcggc gtttgatgag
    17761 gacaacatcg atgtggtgca gtacaccctg cgcaagcggg cagactgagc atggcacctt
    17821 tgcgcatccg tcgacgcact ctgcttggcg caggcgcgca ggcgctcagc ctgggcctac
    17881 tggggctgca cgacgcaagc gcacaacccg aagtgccgct ggagattcgc aacggtgtat
    17941 ccagcgcccg tctggcaggt actgcacgga tgcggttctt cggtctgaat atctacgacg
    18001 cgcggctgtg ggtggcgccg ggttttcagg cgtcagcatt tgctctgcat ccgctggcgc
    18061 tggagctgac ctatctgcgc gcactgaatg gccgctccat cgccgaacgg tcactaaagg
    18121 aaatgcagcg caccgcgccc ctgaccaccg aagtcgccca gcgctggctc ggcctgatgc
    18181 aggacgcgtt tccagatgtc gtagagggcg accggctcac cgggctgcac cggcctggca
    18241 cgggtgccca gttctggttg aacggccagc cacgcgccac cattccagag gcggagttca
    18301 gccgtcggtt ctttggtatc tggctgtccg aagacaccag cgagccgcgg ctgcgtaccg
    18361 agctgctggc gggcaatacg ccctgaggca ggaccatgcg caccccaacc acactgcccc
    18421 tacctgcgac ccccttcacc tggcgtgacg gctggcgcta tgggctgatg gggttcccac
    18481 tggcgtttgt ggctctgccc ctgtatgtca cgcttcccaa ccactacgca cgccacttcg
    18541 gtgtaccgct ggccacactg gggctggtgc tgctgtttgc gcggctcttc gacgcatttg
    18601 tcgatccggt actgggccgc tggggtgatc gcctttacaa gcagtcgccc cgcgccgtgt
    18661 tgcgctgggc ggcgcttgct gcggtgctgc tgggtgcagg atttgccctg ctgttttttc
    18721 cacccgtgca acacccggat gccttgctgg tctgggctgt gggatgcctg ctgctcacct
    18781 acaccgccta cagcacactg agcatcctgc accagtcctg gggtgccctg ctgggcggcg
    18841 atgaggcgca gcgcagccgg gtggtggcct ggcgcgaagg gctggggctg gcaggggtgg
    18901 ttatggcatc tattacgcct gtagccctcg gtttatctgc aacagtagct atatttttta
    18961 tagcacttgc tgtagggtgg tcggcttggc gttgcgcacg acaacccgag ttgacccacc
    19021 ccgcgagcga gttttctggt atcgccgttc gaccagagtc ttccctctgg cacccgtttt
    19081 cgcggccagc attccgggcc ttgctggccg tgtttgtggt caatgggatt gccagtgcca
    19141 tcccggcgac cctgatcctg ttttttgtgc aggaccggct gcaggctcca tccgcggttg
    19201 agcccctgtt tctggggagc tattttgtct gcgccgctct ggccatgcca ctttggctgg
    19261 ccgttgtgcg tcgcctgggg ctggcccgaa cctggggtct aggcatgctg ctgtcgattg
    19321 ctgtgttcgt gttcgcctcc acactgggtg ccggtgatac gacgttgttt gtcatcgtct
    19381 gcgcactttc cgggttcgct ctgggtaccg atctggcgct gcctggcgcc atgctggccg
    19441 ggaccatcgc cgccagtggc gaccggggac acgccgaagg cgcctatttt ggctggtgga
    19501 actttgccac caagctcaac ctggctctgg ctgccggtct ggcgctaccc gcgcttgggc
    19561 tgctgggcta cacaccgggt tcccgcgatc ctgaggcact gcagatgctc acctgggcct
    19621 actgcctgtt gccctgcctg ctcaaagcaa tagccgccgt gctgctgtac acacgcattc
    19681 ttcgaaagcc cctatgaaac gacgcctgct cctgctctct ggtactgccg ccaccgccat
    19741 gctggtgggt ggatgcgcct cacaacaact ggactcctac accgccgaaa agccggttct
    19801 ggacttgcag caatacttca acggtacgct cgacgcatac ggcatcttca ccgaccgctc
    19861 cggtacggtg gtgaagcggt ttacggtcgt catgcgctgt acctggcagg gcaatgaggg
    19921 ggtgctggac gaggacttcc tctactcgga tggcagcaag caacgccgcg tctggcgcat
    19981 caccaagttg cccggcgggc gctataccgg cactgcagac gatgtggtgg gcgttgcgca
    20041 agggcaggcg cagggcaatg cgctgaactg gagctacacc ctgagccttc ctgtcgacgg
    20101 caaagtgtac gaagttcagt ttgacgactg gatgtacctg atgtccgaca ccgtgatgct
    20161 gaacaaggcc accatgcgaa agtttggcgt ccggctgggc gaggtcacgc tcgcattcac
    20221 gaggcgtgcg ccgtgagctt cttccaaccc ctcaacccgc cgctgaagga atggcgcggc
    20281 aagaccgtat ggatcgtcgg cgcttctacc gggattggtc tggcaaccgc cgaagccctg
    20341 cacacgctgg gcgcccgcgt catcgtgtcg gcacgcaagg ccgcttcgct ggacgccttt
    20401 gtggcacacc accctggcag catggcgctg gcactggatg ccacggacag acaggccgtt
    20461 ggtcaggcgg cgcgccaatt gcaggcgctc ggccccctgg actgcgtggt gtactgtgcc
    20521 ggccactaca acgccatgcg tgcatgggcc ctggacccag acgacatgct gcgccacatg
    20581 gaggtgaatt acagcggagc catccatgtg ctggacgctg tgctgcccca actactggcg
    20641 cgcggcgaag gacacatcag tctggttggc agcgtggctg gctaccgggg cctgcccaac
    20701 ggcctggcct acggtccgac caaggcagcc ttgatcaacc tggcggaaac cctatatctg
    20761 gatttgcgtg accgggggat cggtgtatcc atcatcaacc ccggctttgt acaaacgcca
    20821 ctgaccgcag ccaaccagtt tgccatgccg gcgctgctga caccgcgcga ggctgcgacg
    20881 gccatcgtca aaggctgggc agaaggcgcc tttgaaatcc atttccccaa acgtttcaca
    20941 ctgtggatgc aggccctgcg cctgttaccc aaccggttat tcttccaggc cgttcgccgc
    21001 gccacgtcat gaataacgcc aaccacaacc ctcctgccat accgctgcat gcttcccgcc
    21061 tggtggcttt cttcgaaagc ctgacgcctg catcggttgc ggagatggcc cttttttacg
    21121 acccgcaggc gcggttcaag gacccgttca acgatgtcac gggattgccc gccatccagc
    21181 acatctttac ccacatgttt gtggcgctgg actccccccg ctttgtcgtt accaaccaga
    21241 tgacccaggg cgctcagtgc ttcctgacct gggagttccg gtttgcgttt cgcaatttca
    21301 aaaaagggca ggagcaggtc atcctcggct caacccatct ggtgttttca aatgaaggac
    21361 tggtgacgct gcaccgggac tactgggatg ctgcgcagga gctgtatgaa aaactgcccg
    21421 tggtgggcag cttgatgcgc tggctcagga aacgggccaa tagctaggcg aacgtcgagt
    21481 gctgctcaga cgttgaaaga ctgttggctt gaaactcggt ggcacggcgc ggctcccacc
    21541 atgcccgcag cgtgtcaaac acattacgac agccggtgca tttgtactga ccggtcggaa
    21601 ccagggcccc attttcagcg cggccaatga cgcgctggta gctggtcgca ccgcattcgc
    21661 ggcattggta gatcgtcttg tagttcgcca tggtgggcca gactgtacgg ccagcccccg
    21721 cacttggcga gtcaatttat gtaaaccaat gtaagcacct acaaaggtgc ggtgcgtcag
    21781 accggaacgg tgtcggcgaa gctcgggcgc tgcatcagct tgtcgtgcag tttgaccagc
    21841 gcgggatact ccgagcgcca gtccaactgg ggaaagcgga agtccaggta gcccagcgcg
    21901 cagcccacgg cgatatccgc caggctgagg tgaatgccag agcaatacgg cttgtcaccc
    21961 agcccctggt tcatggcttt gacggcatcg ttgaccttgg ccatctggcg atcaatccaa
    22021 gcctgactac gttgcgcctc ggtacgtcca ccccaggtgg cttccagacg cgccagaatg
    22081 gaagcatcca gcaggccatc ggccagcgcc tcccaggttt tgacttcagc gcgctcgcgg
    22141 cccaccgagg gaatgagctt gcctacgggg gacagggtgt cgaggtattc cacgatcacg
    22201 cgggaatcaa acagtgcctc ggcgccttcc atgatcaggc agggcacctt gcccagcgga
    22261 ttgctaaagg ctatggtggt gtctgcgctc cacacgtcct cgggtacaaa ggtgtaatcg
    22321 agttttttct ccgccatcac gacgcgcact ttgcggacat aggggctgga ggtggatccg
    22381 agtagtttca tgggcggctt gctgtttcag gagctttcat tctatccatt gggtcaggct
    22441 ggcgaggggg cggcgggctt tgtggcgcac aacctacaat tcgaaaatga ctttctctgc
    22501 catttctgca ctttccccgc tggacggccg ctacgccgcc aaactgtcca ccctgcgccc
    22561 cctgaccagc gagctgggct acatgcaccg ccgggtgcaa gtcgaggtgg cctggttcat
    22621 tgccctgagc gatgccggct ttgctgaatt caagccgctg agccccggag cgcgcactta
    22681 cttgctgggc ttggtgaaga acttttccga agccgatggc gaagccatca aggcgatcga
    22741 aaagaccacc aaccacgacg tgaaggcggt ggaatactgg atcaaatcca agttcgaggc
    22801 gcggcctgaa ctggaacacg ccagcgaatt tgtccacttt gcctgcacca gcgaagacat
    22861 caacaacacc agccatgccc tgcaactgcg ctcatcgcgc gatgtcgtcg ttctgccggc
    22921 gctggacaag atcatcctga aactgcgcga catggcccat gcctatgccg acgtgcccat
    22981 gctcagccgc acccacggcc agaccgccag ccccaccacc gtgggcaagg aaattgccaa
    23041 cgttgtagtg cgcctgcaga ccgcctgcga acggattgcc aatatcaaga tgctggccaa
    23101 gatgaatggc gccgtaggca actacaacgc ccacctgagc gcctggcccg actttgactg
    23161 ggaagctttc agcaaaaagg tcatcgagac acctgagcct ttgggcttgg gtctgacctt
    23221 ccagccctac agcatccaga tcgagccgca cgattacatg gccgaactgt ttgacgcggt
    23281 agcccgcacc aacaccatcc tgatcgacct gagccgtgac atctggggct atgtgagcct
    23341 gggttacttc aagcagaagc tcaaggacgg cgaagtgggc tcctccacca tgccacacaa
    23401 ggtcaatccg attgacttcg aaaatgccga aggcaacctg ggtctggcca atgcagtgct
    23461 gcgccatttg agcgaaaagc tgcccatcag tcgctggcag cgtgacctga cagactccac
    23521 cgtgctgcgc aatattggcg tggcgctggg ctatgcagtg ctggcctaca gttcgctgat
    23581 gaccggcctg aacaagctgg aaatcaacga agccgccatt gccgaagacc tggactcttc
    23641 ctgggaagtg ctggccgagc cgattcagac cgtgatgcgc cgctttggct tgccccagcc
    23701 ctatgagcag ctcaagaagt tcacccgcgg cgccgccatg acacgcgagc tgatgcaggg
    23761 ctttattgcc gggctggacc tgccccagag cgaaaaagac cgcctgctgg cgatgacgcc
    23821 cggaagctat accggcaaag cctctgagct ggcaagacgc gtctgatggc tcttaaatcc
    23881 accatctaca aagccaacct gcagattgca gacattgatc acagctacta cgccgaccat
    23941 gcgctgaccc tggcgcgcca ccccagcgag accgacgaac gcatgatggt gcgactggtg
    24001 gcgctggcgc tcaacgccta caagctgcaa agcgagtgca atggc
//
LOCUS       DAOSJM010000003        16306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_3734_length_16306_cov_4.579718, whole genome shotgun sequence.
ACCESSION   DAOSJM010000003 DAOSJM010000000
VERSION     DAOSJM010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 16306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16306
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3734_length_16306_cov_4.579718"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PKX82_00255"
     CDS             complement(<1..339)
                     /locus_tag="PKX82_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50702.1"
                     /translation="MKLSNMSLAAKLWLALGVVVGLLVFILVVSGARGAALSARLDAD
                     AAERVIKGREFSRWVGLTETSTTRTTASLMSADPAVTVFYKDSIAAGLQEISDLRKKI
                     LEMNNSEATKA"
     gene            complement(419..1786)
                     /gene="radA"
                     /locus_tag="PKX82_00260"
     CDS             complement(419..1786)
                     /gene="radA"
                     /locus_tag="PKX82_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOF50703.1"
                     /translation="MAKDKTIFTCNECGGISPKWLGKCPHCNAWNTLVESVAHAEVGT
                     KNRFASLARTAEIAVLSEIEASDVDRTPTGHEELDRVLGGGIVEGGVVLIGGDPGIGK
                     STLLLQALDSLQRSGKNTLYVTGEESGAQVALRSRRLGLDHSQVKVLAEIQLEKILAT
                     LDATQPDVAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRAAKASGTAIVLVGHVTK
                     EGALAGPRVLEHMVDTVLYFEGDTHSQFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVS
                     NPSAIFLSQHAEPVPGSCVMVTLEGTRPMLVEIQALVDSGGPSPRRLSVGLDKDRLAM
                     LLAVLHRHAGVACMDQDVFVNAVGGVRISEPAADLAVMLSITSSLRGKPLPKGFIAFG
                     EVGLAGEVRPAPRGQERLKEAAKLGFSVAVVPKANAPKKPIEGLTIHAVERVEQAMEV
                     VRSLT"
     gene            1940..4492
                     /locus_tag="PKX82_00265"
     CDS             1940..4492
                     /locus_tag="PKX82_00265"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOF50704.1"
                     /translation="MPVPPSEAGLSATEAAQRLRTDGPNALPSEQRRGVLSVVRETMR
                     EPMFLLLLAAGLLYLLLGDLQEGLILFGFVLMVLALTIYQEGKTERALESLRDLTSPR
                     AQVVRDGMPMRIAGTDVVVGDLLILSEGDRVPADALLVSGNELQVDESLLTGEAAPVS
                     KIPGTTGVAPVAERPGGDNLPWLYSGTLIVRGHGLARVTATGARSEIGRIGSALDSLT
                     AEASPLKKQTARLIRVLVMVALGASLSLVVVFGVMRGDWLGALLAGIALAMALLPEEF
                     AVVLTVIPAMGAWRLSKQNVLTRRIAAIETLGAASVLCVDKTGTLTENRMTVAQLHVD
                     QGIVGPQTLDIDYSAPAELAEEFHALVEYSILASMAEPFDPMEKAFHRLGQHFLQGTE
                     HLHRDWTLVQEYGLTPQLRAMSHVWRAVQGEDHVVAAKGAPEAVMDLCHLDAPTQARI
                     EQAINTMAAQGLRVLGVASARFAGEAWPAQEHDFDFAFLGLLGLADPLRAEIPAAVAQ
                     CHTAGIRVIMITGDYPATASAIAVKAGLRVEGECSVLTGDAIAGLSDAALQEHMQTVS
                     VCARVAPEQKLRIVQALQARGAVVAMTGDGVNDAPALKAAHVGVAMGGRGTDVAREAA
                     ALVLLDDNFASIVGAVQLGRRIFDNLRKSMSYILAVHVPIAGMAMLPVLFGWPVMLYP
                     LHIAFLELVIDPACSMVFENEPLEADAMQRPPRDPQAMLFGGMTLVMALLQGLGVLLV
                     ACGATLWGRTWLSEDALRAFVFVTLVAGNLALIFSNRSGTGSLWAMLRVPNRTLWIVS
                     AVTLGMMVMAVYVPWLSGLFHFEALNGSDLLLAIAMGAMSVLWFALVKRSRS"
     gene            complement(4489..5697)
                     /locus_tag="PKX82_00270"
     CDS             complement(4489..5697)
                     /locus_tag="PKX82_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50705.1"
                     /translation="MKYVPIPVAMLEVGKPLPVNVVSDTGQLLLRKGQPIVSEQHRDK
                     LHAFNASTSPSDAQAWQRAYERMVHELLRSGVDVQEIARMPMPAEIRESDYVVGRQLH
                     GGWLDLQEVLRGILYQGGLAINPLQRLADIQKKAMSLLHADADDSLFCLYQALADDSL
                     GYCATHALLCAVMCELTATKLGIDTAHRHSLVAAALTMNIGMARDQDSMARQSSDITE
                     WQRTLIAEHPARGADILAGFGVDDVDQLDIVRWHHAPDHANAYPANHAARRLLALADS
                     FVARTTARKTRASQSAVKAVKAMVLKAQGDELGVGSAMAQAVGFYPPGSYVQLVTGEM
                     AISVMRGQRANMPWVITIADKDGIPAVKYQCKDTSDPALAIAAPVNYEKVKVAVSADK
                     VRRARERIPR"
     gene            complement(5694..6788)
                     /gene="alr"
                     /locus_tag="PKX82_00275"
     CDS             complement(5694..6788)
                     /gene="alr"
                     /locus_tag="PKX82_00275"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOF50706.1"
                     /translation="MPRPIQATIHTQAFVNNLAVARRAAPDARVWAVVKANAYGHGIE
                     RVFDGLRGADGFALLDLAEAQRVRDLGWRGPILLLEGVFELRDLELCSRLDLWHTVHC
                     DAQIDMLAAHKTNVPHRVFLKMNSGMNRLGFAPDQYRSAWTRLNALPQVDEISLMTHF
                     GNADGPLGIADAMAVFAAVTSDLPGERTLSNSAATLRHPDAVQVSDWVRPGIAIYGSA
                     PDYPEHTAAHWGLQPTMTLAAKIIAVQAISARAGVGYGSNFVAQEAMRIGVVACGYAD
                     GYPRICPTGTPVLVDGVRSRTLGRVSMDMLAVDLTPVPDAGQGSEVTLWGRAANGAVL
                     PIDEVARQAQTVGYELMCALAQRVPVVVAE"
     gene            6914..8197
                     /gene="lplT"
                     /locus_tag="PKX82_00280"
     CDS             6914..8197
                     /gene="lplT"
                     /locus_tag="PKX82_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HOF50707.1"
                     /translation="MKRGFFTIMSAQFFSSLADNALFVAAVQLLRTGGAPEWQQAALV
                     PMFALFYVVLAPFVGAFADALPKGKVMLISNSIKVVGCLLMLFGTHPLMAYAIVGLGA
                     AAYSPAKYGILTELLPASQLVKANGWIEGLTIASIILGVLLGGQLVGTVISTKLLALD
                     FPLLTTSVDTAPEAAISVLIFVYLLAAWFNTKIPLTGVPIRALPRNLVTLLPDFWHCN
                     TRLWRDKLGQISLAATTLIWGVAGNLRFIVLAWAAAALGYSVTQASALQGVVAIGMAV
                     GAVVASLRMRLEDGPRVIPLGIGMGMLIVLLIFIDNVWVAAPFLILLGALGGFLVVPM
                     NALLQHRGHNLMGAGRSIAVQNFNEQACILLLGAAYSLSTGLGMPTFGAITAFGVVIA
                     GVMWLIKRWHERNCRNHTVELENLLEIARSDNVHG"
     gene            8190..9074
                     /locus_tag="PKX82_00285"
     CDS             8190..9074
                     /locus_tag="PKX82_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF50708.1"
                     /translation="MGNVRAALALTVNAFAWGVSWWPFRQLEDLGVHPLWATAMVYLV
                     ALVFLLLAQPDAWRGMLRFPGLWLLALAAGLTNVGFNWAVTQGDVVRVVLLFYLMPGW
                     VVLLAWPLLGERPHRASMFRLALALAGVIVVLKTPEVAWPWPESLPDWLALMGGAAFA
                     LTNIFLRKFNHTPQSATMFAMFGGSAAMTLGTALLGLGLGIVPSLPGVHTEWLLWVSG
                     LSLLILAGNLGLQYGAARLSANATSIIMLSEVVFASTSAVLLGASTVTPRILLGGAMI
                     LAAAILSALHTSPRSTHG"
     gene            9148..9633
                     /locus_tag="PKX82_00290"
     CDS             9148..9633
                     /locus_tag="PKX82_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOF50709.1"
                     /translation="MPSIYDFEALQISGTSVPLKQYQGKAMLIVNTASACGFTPQFAG
                     LEELHKAYGDKGLVVLGFPCNQFGSQDKGSNDEIAEFCQLNYGVSFPMMAKIDVNGSG
                     AHPLYQYLTKEAPGLLGSKAIKWNFTKFLIGKDGSVLKRYAPTDTPKSLAGDIERALA
                     A"
     gene            complement(9643..11583)
                     /locus_tag="PKX82_00295"
     CDS             complement(9643..11583)
                     /locus_tag="PKX82_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOF50710.1"
                     /translation="MPVNQTDSWAKTAPIPLQPAPDTARPAARNMGELRVLLDAARHA
                     PIRSLGESLLDLKLIDMDTLARYCEHPPQVERSVEHLLTESGLVKELEIEHARAHMLN
                     TPEVDAEKFTVEREALDHLAWNVAFTHHVVPLGMESGMLYVASSTPLKRELEVELSVF
                     ANCMVSLVWASRAQIVRRLAKETHTQTSLEVALASGFDELSRSGEQLTADVHSLLSDA
                     MGEMKGATDPEQVSATDESSSVVRLVNRMIMDAYEQKASDIHIETNPGDDVSRVRFRK
                     DGDLEDYLVLPFLLRAAVVSRIKIMSRLDISERRRPQDGKINFHDFADIKLELRVAIL
                     PTHDNLEDVVMRLLATSKPIPLQQLGFSARDDKIIKRLTERQFGLILACGPTGSGKTT
                     TLHSLLAHINTDSRKIWTAEDPIEITQKGLRQLQVNPKIGVTFASAMRAFLRADPDVI
                     MIGEVRDEETASVCIEASLTGHLVLSTLHTNSAAESVIRLLDLGMDPMNFGDSLIGIV
                     AQRLVRALCKHCAVPKVLTGAELEALAQEYMEGTSLKMEEASQRLLEAAALASGDREA
                     PVRMYHPVGCERCGGRGYKGRMGIYELLENTGGMKQKIQSRAATSDIFQEATRAGMRT
                     LRQDAIEKVLAGYIDLAQSRLV"
     gene            complement(11681..12970)
                     /locus_tag="PKX82_00300"
     CDS             complement(11681..12970)
                     /locus_tag="PKX82_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOF50711.1"
                     /translation="MNYLYLYALVFGVVLVLYMRRQSRIHKTHKTELQESIQSGLAEP
                     PSLHPVVDPVRCIGSGSCATVCPEDALGIVNGKAVLKNAAHCIGHGACMAACPFDAIK
                     LVFGTEKRGIDIPNISQDFETNVPGIFIAGELGGMGLIRKAAEQGRQAIAAIRRKTSG
                     QGDYDVVIVGCGPAGLSAGLSSIHHKLKYKLMEQEDSLGGAVYHYPRQKIAMTAPVVL
                     DLIGTVKFGEVQKEKLLEFWQGVVEKTGLQIGFRERMEGVERTAQGFVVRTNKGAYAT
                     RTVLLTMGRRGTPRKLDVPGEETAKVVYRLVDPEQYRGQAALVVGGGDSALEAAIALA
                     EQPKTSVILSYRSAAFSRVKQKNRTLLEQAEKDGRIQVMLNSTVSEIFDHEVEIEQNG
                     TRQRFPNAVVIVCAGGLLPTPLLQKIGIEFATKFGTA"
     gene            complement(12986..14194)
                     /locus_tag="PKX82_00305"
     CDS             complement(12986..14194)
                     /locus_tag="PKX82_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50712.1"
                     /translation="MDNPIDAIKRQLAQLKELHESGVLSQPQYEEGKTTLERRLLDLV
                     LSGATVPVVPVESVAQTLAHDASRTAARPSGLLVAALVAGIVAIAAAGYWWKGSPSLT
                     SQPMAQAPGAGNTEGTSGAAHATDSEQIAAMTEKLAARMKEQPDDAEGWAMLARSYSV
                     LGKHPEALKAYEKAVGLRKDDATLLADYADSLAVKNNRALDGEPMRQVERALKLDPRN
                     LKALSLAGTHAFNKKDYAGAVRNWEKVVQFGAADNTLVQQIQPGLAEARNLAGLPAAP
                     SLDSGKNAVPAEATVSGTVTLAPELRKLAQPEDTVFVFARAAAGSKMPLAILRKQVKD
                     LPLQFTLDDSMAMTPANALSGSPQVIVGARVSKSGNAMPQPGDLSGQTGAVKVGATGV
                     KLEIKETVKP"
     gene            complement(14217..14666)
                     /locus_tag="PKX82_00310"
     CDS             complement(14217..14666)
                     /locus_tag="PKX82_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOF50713.1"
                     /translation="MAKWILWMSLPFWVGFAFANEAAPASADPALEARMMRISSELRC
                     LVCQNQTIADSHAGLAVDLRQQVREMLQKGQSEQEIIAYMTARYGDFVLYRPPFKATT
                     ALLWIGPGVMVVAGLAILVIVLRRRSRMPQDQFDPESPDAGQDPHLS"
     gene            complement(14659..15189)
                     /locus_tag="PKX82_00315"
     CDS             complement(14659..15189)
                     /locus_tag="PKX82_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HOF50714.1"
                     /translation="MKYLRFLIPLFVFLGLVWFLARGLGLDPKTVPSPLIGKSAPGFS
                     LTKLDNPEKTIRREDLLGKVWMLNVWASWCVACREEHPLLVEFSRKKLLPIYGLNYKD
                     DRAAGLRWLANFGNPYDASLFDNDGRVGIDWGVYGVPETFIVDREGIIRFKQIGPLTP
                     EVIRTQIEPLVRQLNG"
     gene            complement(15186..>16306)
                     /locus_tag="PKX82_00320"
     CDS             complement(15186..>16306)
                     /locus_tag="PKX82_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HOF50715.1"
                     /translation="ILAFSFSLLGTFLVRSGVLSSVHAFATDPRRGLFILAFLTIVVG
                     ASFALYAWRAPTVGLGARFALMSKETALLANNVLFLVAAGAVLLGTLYPLLLDALGMG
                     KISVGPPYFDNVFMPLMAPTVFLMGIGPMARWKEADVPALAKRLRWALGVTVVAAIST
                     GLLAGEIHLLATCGLLMAYWIFTSVATDLWERIQPEGRGLTEIRRKWRQLPRALIGMM
                     VAHIGVAVFTFGVVMVKTYEVESDVKMNVGDSTTVRGYTFTFKGVREIKGPNYVAAQG
                     QMEITRGRDRIAELQPEKRVYRVQQNPMTEAAISTGLTRDLYVSMGEQVDDGAWIVRV
                     YVKPFIDWIWGGCMLMALGGLMAVSDRRYRRKQKVATA"
BASE COUNT         2993 a         5224 c         4853 g         3236 t
ORIGIN      
        1 cgcttttgtt gcctcggagt tgttcatttc cagaatcttc ttgcgcaggt cgctgatctc
       61 ctgcaagccc gctgcgatcg agtccttgta aaaaacggtc actgcagggt cggcactcat
      121 cagcgatgcc gtcgtgcggg tggtactggt ttctgtcaac ccgacccagc gcgaaaactc
      181 ccgccccttg atgacgcgct cggccgcatc ggcatccagc ctcgcactca gggccgcacc
      241 gcgcgcgcca gacaccacca gaataaatac cagcaggcca accaccaccc ccagggccag
      301 ccagagtttg gctgccagtg acatattgct caatttcatg tacgtatctc cattcagagg
      361 atcatggtaa ccacggccct ccctttacgc aatgacaacc cgcaagtgtc gaactgactc
      421 aggtcaggga gcgcaccacc tccatggcct gctctacccg ctccaccgca tggatggtca
      481 ggccctctat cggcttcttg ggcgcattgg ccttgggcac cacggccacg ctgaagccca
      541 gcttggcggc ttctttcagg cgttcctggc cccgcggcgc cgggcgcacc tcgccggcca
      601 gccccacttc accaaaggca atgaagcctt tgggcagggg cttgccgcgc aggctggagg
      661 taatggacaa catcaccgcc agatcggcgg ccggctcgct gatgcgcaca ccgcccaccg
      721 cattcacaaa cacgtcctgg tccatgcaag cgacccccgc atggcggtgc aacaccgcca
      781 gcagcatggc cagacggtct ttgtccaggc ccacgctcaa gcggcgcggg ctggggccac
      841 cactgtcgac cagcgcctga atttcgacca gcatcgggcg cgtgccctcc agcgtcacca
      901 tcacgcaact gccgggcacc ggctcggcat gctggctcag aaaaatggcg ctggggttgg
      961 acacgccctt gagacctttc tccgtcatgg caaacacccc gatttcgttg accgcaccaa
     1021 agcggttctt gatggcgcgc accaggcgga actggctatg cgtgtcaccc tcaaagtaca
     1081 gcaccgtgtc caccatgtgt tcgagcacgc gcggacccgc cagcgcaccc tccttggtca
     1141 cgtggcccac cagcacgatg gcggtcccac tggccttggc cgcacgcgtg agatgcgccg
     1201 cacattcgcg cacctgcgcc accgagcccg gggcgctggt gagctggtcg gaatacacgg
     1261 tctggatgga gtcgatcact gccacatcgg gctgcgtggc atccagggtg gcgaggattt
     1321 tctccagctg gatctcggcc agcaccttca cctgcgagtg gtccagcccc aggcggcgcg
     1381 accgcagagc cacctgtgca ccgctttctt caccggtcac atacagtgtg tttttgccgc
     1441 tgcgctgcag cgaatccagc gcctgcagca agagcgtgga tttgccaatg ccggggtcgc
     1501 ccccgatcag caccacgccg ccttcgacga tgccaccgcc cagcacccgg tccagctctt
     1561 catggccggt gggggtgcgg tccacatcgg aggcctcaat ctcgctgagc accgcaattt
     1621 cggccgtcct ggccagggag gcaaagcggt ttttggtgcc cacctcggca tgggccacgg
     1681 actcgaccag cgtgttccag gcgttgcagt gggggcactt gcccagccac ttggggctga
     1741 tgccgccgca ttcgttgcag gtaaaaatgg ttttgtcttt ggccatgggc caataatact
     1801 gtatatattt acatgagttc gcccattttt ccagaatgag catgctatgc tatttatagc
     1861 tatccacgca caattcacgg tggctacggc ctgtttttgc ttcaaatcca tctatcatcg
     1921 aggctgaagg agccccacca tgccggtacc accgtctgaa gcaggcctga gcgccaccga
     1981 ggccgctcag cgcctgcgca ctgacggacc caacgccctg ccatcggagc agcgccgtgg
     2041 cgtgctgagc gtggtccgcg agacgatgcg tgagcccatg ttccttctgc tgctggcggc
     2101 cggcttgctg tatctgctgc tgggagacct gcaggaaggc ctgatcctct ttgggtttgt
     2161 gctgatggtg ctggcactca cgatttacca ggagggcaaa accgaacggg cattggaatc
     2221 cctgcgggac ctgaccagcc cccgggcgca ggtggtgcgg gacggtatgc cgatgcgcat
     2281 tgcgggcacc gatgtggtgg tgggtgacct gctgatcctc tccgaaggcg accgcgtgcc
     2341 tgcggatgcg ctgctggttt cgggcaacga actgcaagtg gatgaatcgc ttttgactgg
     2401 tgaggctgcg ccggtgagca aaatcccggg caccaccggg gtggcgcccg ttgccgaacg
     2461 cccgggtggc gacaacctgc cgtggctgta ttcgggcacg ctgatcgtgc ggggccacgg
     2521 actcgcccgc gtaaccgcca ccggtgcacg cagcgagatc ggtcgtatcg gctcagcact
     2581 cgactccctt actgcagagg cttcgccgct gaaaaaacag acggccaggc ttatccgggt
     2641 gctggtgatg gttgccctgg gcgccagtct gtccctggtg gtggtgttcg gcgtgatgcg
     2701 gggcgactgg ctcggcgcac tgctggccgg cattgcgctg gccatggctt tgctgcccga
     2761 agaatttgct gtggtcctga cggtcattcc cgcgatgggt gcctggcgtc tgtcgaagca
     2821 aaacgtcctg acccggcgca tcgccgccat cgaaacgctg ggggccgcat cggtgctgtg
     2881 cgtggacaag actggcaccc tgaccgaaaa ccgcatgacg gtggcccagt tgcatgtcga
     2941 ccaaggcatt gtcggcccgc agaccctgga cattgactac agtgcaccgg ccgagctggc
     3001 ggaagagttc catgctttgg tggagtactc cattctggcc agcatggccg agcccttcga
     3061 cccgatggag aaggcgtttc accggctcgg gcagcacttc ctgcagggca ccgaacacct
     3121 gcaccgggat tggaccctgg tgcaggagta cgggctgacc ccccagctgc gggccatgtc
     3181 ccacgtatgg cgcgccgtgc agggcgaaga ccacgtggtg gccgccaagg gcgcccccga
     3241 agcggtgatg gatttatgcc acctggacgc ccccacccag gcccgtattg agcaggccat
     3301 caacaccatg gcagcgcagg gcctgcgggt actgggcgtg gccagtgcac ggtttgcggg
     3361 tgaagcgtgg cccgcacagg aacacgactt tgactttgcg tttttgggtc tgctggggct
     3421 ggccgatccg ttgcgggcag aaattcctgc ggccgttgcg cagtgccata ccgcgggtat
     3481 ccgcgtcatc atgatcaccg gggactaccc tgccaccgcc agcgccatcg ccgtgaaggc
     3541 gggtttgcgc gtggagggcg aatgcagcgt cttgacaggc gatgccatcg ccggcctcag
     3601 cgatgccgca ctgcaggagc acatgcagac agtcagcgtg tgcgcccggg tggcgcccga
     3661 gcaaaagctg cgcattgtgc aggcgctgca ggcgcgcggt gcggtcgtcg ccatgacggg
     3721 cgatggcgtc aacgatgcgc cggcgctcaa ggccgcccac gtgggcgtgg ccatgggcgg
     3781 ccgggggacc gatgtggccc gcgaggcggc cgcactggtg ctgctggacg acaactttgc
     3841 atccatcgtg ggggcagtgc agctgggacg gcgcattttt gacaatctgc gcaaatcgat
     3901 gtcctacatc ctggccgtgc atgttcccat cgcaggcatg gccatgctgc cggttctgtt
     3961 cggctggccg gtgatgctgt acccgctgca cattgcgttt ctggaactgg tcattgaccc
     4021 ggcctgctcg atggtgttcg agaacgagcc gctggaggca gacgccatgc aacggccacc
     4081 gcgcgatccc caggccatgc tgtttggcgg catgacgctg gtgatggccc tcttgcaggg
     4141 gctgggggtc ttgctggtgg cgtgcggtgc caccctgtgg ggccgcacct ggcttagcga
     4201 agacgccctg cgcgcctttg tatttgtgac gctggtcgcg ggcaatctgg cattgatctt
     4261 ctccaaccgc tccggtaccg gctccctgtg ggccatgttg cgggtgccca atcgcacgct
     4321 gtggattgtc agcgccgtga cgctgggcat gatggtgatg gcggtctatg tgccctggct
     4381 gtccgggctc ttccattttg aagcgctaaa cgggagcgac ctgttgctgg ccatcgccat
     4441 gggtgccatg agcgtgctgt ggtttgcgct ggtcaagcgc agccgcagtt agcgcgggat
     4501 gcgctcgcgt gcccgacgca ccttgtcggc actgacggcg accttgacct tttcgtagtt
     4561 caccggtgcc gcaatggcca gcgccgggtc cgaagtgtcc ttgcactggt acttcaccgc
     4621 cggtatgcca tccttgtcgg caatggtgat gacccacggc atattggcac gctggccacg
     4681 catgaccgag atcgccattt cgcccgtcac cagctgcacg tagctgcccg gagggtaaaa
     4741 cccgaccgcc tgcgccatgg ctgaacccac gcccaattcg tccccctggg ccttgagcac
     4801 catggccttg accgccttga cggcagattg cgatgcacgg gtcttgcggg ccgtcgtgcg
     4861 ggccacaaaa ctgtcggcca gcgccagcag gcgcctggcg gcatggttgg ctggataggc
     4921 attcgcatgg tcgggcgcgt ggtgccagcg cacgatgtcg agctggtcca cgtcatcgac
     4981 cccaaacccg gcgaggatgt ccgctccgcg cgcggggtgt tcggcaatca gcgtgcgctg
     5041 ccactcggtg atgtccgaac tctggcgcgc catgctgtcc tggtcgcgcg ccatgccgat
     5101 gttcatggtc agcgcagctg ccaccagcga atggcggtgc gccgtatcga tgcccagctt
     5161 ggtggcggtc agctcacaca tcaccgcaca cagcagtgca tgggtggcac agtagcccag
     5221 cgagtcatcg gccagcgcct ggtacaggca aaacaggctg tcatccgcgt cggcatgcag
     5281 cagggacatg gcttttttct ggatgtccgc cagccgctgc agggggttga tggccagacc
     5341 gccctggtac aggatgccac gcagcacctc ctgcaggtcc agccagccgc cgtgcaactg
     5401 gcgtcccacc acatagtcgc tctcgcggat ctcggctggc atgggcatgc gtgcaatctc
     5461 ctgcacatcc acaccgctgc gcagcagctc atggaccatg cgctcgtagg cacgctgcca
     5521 ggcctgggca tcactgggcg aggtcgaggc attgaaggca tgcagcttgt cgcggtgctg
     5581 ctccgacacg atgggctggc ccttgcgtaa cagcagctgt ccggtatcgc tgaccacatt
     5641 gacgggcagt ggcttgccca cttcgagcat cgccacggga atgggaacgt atttcactct
     5701 gccaccacca caggaacgcg ctgcgccagt gcacacatca actcataacc tacggtctgc
     5761 gcctgccggg ccacttcatc gatgggcagc acggcaccgt tggccgcacg gccccacagc
     5821 gtgacttcac tgccctgccc ggcatcgggc acgggcgtca gatccaccgc cagcatgtcc
     5881 atgctcaccc gccccagcgt gcggctgcgt acgccgtcga ccagcaccgg tgtgccggtg
     5941 gggcagatgc gcgggtaacc gtcggcatag ccacacgcca ccacgccgat acgcatcgcc
     6001 tcctgcgcca caaagttaga gccatatccg actccagccc gcgcggatat tgcctgaaca
     6061 gctatgattt ttgcagcaag cgtcatggtc ggctgcaggc cccagtgcgc ggccgtgtgc
     6121 tcgggatagt ccggcgcgct gccatagatg gcgatgccgg gccgcaccca gtcggacacc
     6181 tggaccgcat ccgggtggcg cagggtggct gcgctgttgc tcagcgtgcg ctcgccgggc
     6241 agatcgctgg tcaccgcggc aaataccgcc atcgcatcgg cgatacccag tggaccgtcc
     6301 gcgttgccaa agtgggtcat cagggaaatc tcatccacct gcggcaaggc gttcaggcgg
     6361 gtccaggccg agcggtactg gtcgggcgca aaacccagac ggttcatgcc ggaattcatc
     6421 ttcaggaaca cccggtgcgg cacattggtc ttgtgcgccg ccagcatgtc gatctgcgca
     6481 tcgcagtgca ccgtgtgcca gaggtccagc cgcgaacaca gctccaggtc gcgcagctcg
     6541 aacacgcctt cgagcagcag gatcgggccg cgccagccca ggtcgcgcac gcgctgcgcc
     6601 tcggccagat cgagcagcgc aaagccgtcg gcaccgcgca ggccgtcaaa cacgcgctcg
     6661 atgccatgcc cgtaggcatt ggccttgacc acggcccaca cccgtgcatc gggggctgcc
     6721 ctgcgcgcaa cggccaggtt attgacgaag gcctgggtat ggatggtggc ttggatcgga
     6781 cggggcatgg tggcattgtc aatcggaatg gacaggattc tgacactgcc cggcgtgcta
     6841 taacctcgac acgtttggag acactctaca aaacaatcgg catcggatgt cctgatgccc
     6901 gccatcccga tacatgaaac gcggtttttt caccatcatg tcagcgcagt ttttcagctc
     6961 gctggccgac aacgcactgt ttgtggcagc agtgcagcta ctgcgaaccg gcggtgcccc
     7021 cgagtggcaa caggccgcgc tggtcccgat gtttgcgctg ttctatgtgg tgctggcccc
     7081 tttcgtcggc gcctttgcgg acgccctgcc caagggcaag gtcatgctca tcagcaactc
     7141 catcaaggtg gtgggctgcc tgctgatgct ctttggcacc cacccgctca tggcctatgc
     7201 catcgtcgga ttgggcgcgg cggcctattc gccagccaag tacggcatcc tcaccgagct
     7261 gctgccggcc tcccaactgg tcaaggccaa cggctggatc gagggcctga ccatcgcctc
     7321 catcattctg ggcgttctgc tcggcggaca actcgtgggt acggtgatct ccaccaaact
     7381 gctggcgctg gactttccac tcctcacgac cagcgttgat acggcccccg aagcggccat
     7441 cagcgttctg atttttgttt acctgctggc ggcctggttc aacaccaaga ttccgctcac
     7501 cggtgtgccc atccgcgcgt tgccccgaaa cctggtcacg ctgctgccgg atttctggca
     7561 ctgcaacacc cgtctgtggc gcgacaaact gggccagatt tcactggcgg ccaccaccct
     7621 catctgggga gtggctggca acctgcgatt tatcgtattg gcctgggctg cggcggcatt
     7681 gggttacagc gtgactcaag cgtcggctct acagggggtg gtggcaattg gcatggccgt
     7741 gggcgccgta gtcgcctcgc tgcgcatgcg actggaagac ggcccgcgcg tcattcccct
     7801 gggcataggc atgggcatgc tgatcgtgtt gctgatcttc atcgacaacg tctgggtagc
     7861 agcaccattt ctcatcctgc tgggcgcctt gggtggcttt ctggtggtgc caatgaacgc
     7921 cttgctccag caccgtggac acaacctgat gggtgccgga cgttccattg cagtacaaaa
     7981 tttcaatgag caggcttgca ttctgctcct gggcgccgcc tacagcctgt ctaccggatt
     8041 gggcatgccc acctttggcg ccatcacggc gtttggcgtg gtcattgccg gtgtcatgtg
     8101 gctcatcaag cgctggcatg agcgcaactg ccgcaatcac acagtcgaac tcgagaatct
     8161 gctggaaata gcgcgcagcg acaacgtgca tgggtaatgt cagggccgcc ctggccctga
     8221 ccgtcaacgc ctttgcctgg ggggtttcct ggtggccttt tcggcaactg gaggatctgg
     8281 gcgtgcaccc gctgtgggct acggccatgg tgtacctggt tgccctcgtg tttttgctgc
     8341 ttgcccaacc cgatgcctgg cgcggcatgc tgcgctttcc gggcctgtgg cttctggcac
     8401 ttgccgccgg actgaccaat gtgggtttta actgggccgt aacgcagggc gatgttgtcc
     8461 gtgtggtcct gttgttctac ctgatgccgg gctgggtagt cctgctggcc tggcctctcc
     8521 tgggcgagcg gccccatcgg gcttcgatgt tccggctggc ccttgcgctg gcgggcgtca
     8581 tcgtcgtgct caagacgccg gaggtggcct ggccctggcc ggagtccctg ccagactggc
     8641 tggcgctgat gggtggcgcg gccttcgcac tgaccaacat cttcctgcgc aagttcaacc
     8701 atacgcctca gtctgcaacc atgttcgcga tgtttggtgg aagtgcggcg atgactttgg
     8761 gtactgcgct gcttggactg ggcctgggga tcgttccatc cctgcccggc gttcacacag
     8821 agtggctgct gtgggtgagc ggattgagtc tgctgattct tgcgggcaac ctggggctgc
     8881 agtacggtgc tgcacgcctg agtgccaacg cgacctcgat catcatgctg tctgaagtgg
     8941 tcttcgccag cacatcggca gtcctgctgg gagcatccac cgtgactccg cgcatcctgc
     9001 tgggtggcgc catgattctg gccgcggcga ttctgagcgc cctgcacacg tcgcccagga
     9061 gcacccacgg ctaacgccta gtcctaccgc tgcaagtggt attgatcggt gctatcgtcc
     9121 gccactcctt ccatgaacca gcccaccatg ccatccattt acgacttcga agccctgcag
     9181 atcagcggca catccgtccc cttgaagcaa taccagggca aggccatgtt gatcgtcaac
     9241 accgcaagtg cctgcggatt cacgccgcaa tttgccggac tggaagagtt gcacaaagcc
     9301 tatggtgaca agggtctggt ggtgctcggg tttccgtgca accagtttgg ttcgcaggac
     9361 aagggcagca acgacgaaat cgcggaattc tgccagctga actatggggt gagctttccc
     9421 atgatggcca agatcgatgt caacggctcc ggcgcccacc cgctgtacca gtacctgacc
     9481 aaggaagccc ccgggctact gggcagcaag gccatcaaat ggaacttcac caagttcctg
     9541 atcggcaagg acggatccgt gttgaaacgc tacgccccca ccgacacacc caagagcctg
     9601 gccggggata tcgaacgcgc gctggccgcc tgagcgcctg gtctagacca ggcgggattg
     9661 cgccagatcg atgtaccccg ccaggacttt ttcaatcgca tcctggcgca gggtgcgcat
     9721 gccggcccgg gtagcctcct ggaagatgtc cgacgtggcc gcgcgcgact ggatcttctg
     9781 cttcatgcca cccgtgtttt cgagcagctc gtagatgccc atacggccct tgtagccccg
     9841 cccgccgcaa cgctcgcacc cgaccgggtg gtacatgcgc acgggcgctt cgcggtcgcc
     9901 cgaagccagc gccgccgctt ccagcaggcg ctggctggcc tcttccatct tcaggctggt
     9961 gccttccatg tactcctgtg ccagggcttc cagctcggct ccggtcagca ccttcggcac
    10021 cgcacagtgc ttgcacagcg cacgcaccag acgctgggcc acgatgccga taagggagtc
    10081 gccgaagttc atcgggtcca tcccgaggtc cagcaggcgg atcacgctct cggccgcact
    10141 gttggtgtgc agcgtggaca gcaccaggtg cccggtcagc gacgcttcaa tacacacact
    10201 ggcggtttcc tcgtcccgga cttcgccgat catgatgacg tccgggtccg cgcgcaggaa
    10261 cgcgcgcatg gcggacgcaa aggtcacccc gatcttggga ttgacctgca gctggcgcaa
    10321 gcctttctgg gtgatttcaa tcgggtcttc cgccgtccag attttgcggc tatcggtatt
    10381 gatatgtgcc agcagcgagt gcagggtggt ggtcttgccc gaaccggtcg gaccacaggc
    10441 caggatcaag ccgaactgcc gctcggtcag ccgcttgatg atcttgtcat cgcgtgccga
    10501 gaagcccagt tgctgtagcg gaatcggctt ggacgtggcc agcaagcgca tgaccacatc
    10561 ttccaggttg tcgtgcgtgg gcaggatggc cacacgcaat tcgagcttga tatcggcaaa
    10621 atcatggaaa ttgatctttc catcctgggg ccgacgccgc tccgaaatgt cgagccggct
    10681 catgatcttg atgcgcgata ccacagctgc ccgcaacaga aacggcagga ccaggtagtc
    10741 ctccagatca ccgtccttgc ggaaacgcac gcgtgacaca tcgtcgccgg gattggtctc
    10801 gatatggatg tcggacgctt tctgctcgta ggcatccatg atcatgcggt tgaccagacg
    10861 gaccacgctg gagctttcgt cggtcgcact cacctgctcg ggatcggttg cgcccttcat
    10921 ctcgcccatc gcatccgaca gcaggctgtg cacgtcggca gtcagctgct ctccggagcg
    10981 ggacaactcg tcaaaccccg aagccagtgc cacctccaga ctggtctggg tgtgtgtttc
    11041 cttggccagg cgccgcacaa tctgtgcgcg cgaggcccac accagggaca ccatgcagtt
    11101 ggcgaagacg ctcaactcca cctccagttc gcgcttcagg ggcgtcgagg acgccacgta
    11161 cagcatgccc gactccatgc ccagcggcac cacatggtgg gtgaaagcca cattccaggc
    11221 cagatggtcc agtgcttcac gctcgacggt aaacttttcc gcatccacct cgggagtgtt
    11281 gagcatgtga gccctggcgt gctcgatttc cagttccttg accaggcccg actcggtcag
    11341 caaatgctcg acgctgcgct ccacttgcgg cggatgctcg cagtaccgcg ccagggtgtc
    11401 catgtcgatg agcttgaggt ctagtagcga ctcgcccagc gaccgtatcg gcgcgtggcg
    11461 cgcggcatcc agcaggacgc gcaactcccc catgttccgg gctgcgggac gcgcagtgtc
    11521 tggcgctggc tgcaaaggga tcggtgccgt cttggcccaa ctatcggttt ggttcactgg
    11581 cacgcagttc gctccaacga gatgatttcc atatttccag tgtagaccgt acggggtatc
    11641 tctggacagc aaaaaaattg actattgtca atttctttgc tcaggctgta ccgaattttg
    11701 tcgcaaactc gatgccgatc ttctgtagca gcggcgtagg cagcaatcct ccggcgcaaa
    11761 cgatcaccac ggcattcgga aacctctgtc gcgtgccatt ctgctcaatt tccacctcat
    11821 ggtcgaaaat ctcggacacg gtggagttca gcatgacctg gatgcgtccg tccttctccg
    11881 cctgctccag cagtgttctg ttcttctgtt tgacccggga aaaggcggcg ctgcgatagg
    11941 acaggatgac ggacgtcttg ggttgctctg ccagcgcaat ggccgcttcc agtgcgctat
    12001 cgccaccgcc gacgaccagt gcggcctggc cccggtactg ttcgggatcc accagccggt
    12061 agaccacctt ggccgtttcc tcacccggta cgtcaagctt gcgcggcgta ccccgtcgac
    12121 ccatggtgag cagcacagta cgtgtcgcat aggcaccttt attggttcgc acgacgaacc
    12181 cctgcgcggt gcgctccact ccctccatgc gttcgcgaaa gccaatttgg agtccggttt
    12241 tctcgacgac gccctgccag aactccagca gcttttcctt ctgcacctcg ccgaacttga
    12301 ctgtgccaat caaatccagc acaaccggtg ccgtcatcgc aattttctgg cgcgggtagt
    12361 ggtacaccgc cccgccaagc gagtcttcct gctccatcag cttgtacttg agcttgtggt
    12421 gaatggagga aaggcctgcc gacaacccgg ccgggccaca acccacgatg accacgtcat
    12481 agtcaccctg accactggtc tttctccgga tggccgctat ggcctgccgg ccctgctctg
    12541 ctgctttgcg aatcaggccc ataccgccga gctcgcctgc aatgaaaatg ccgggcacgt
    12601 tggtctcgaa gtcctggctg atattgggaa tgtcgatgcc gcgcttctca gtgccaaaca
    12661 ccagcttgat ggcatcgaag gggcaggccg ccatacaggc accatgcccg atgcaatggg
    12721 ctgcattctt caacacggcc ttgccattga cgatgcccaa agcgtcttcc gggcacaccg
    12781 tcgcacacga tccagagcct atgcaacgga ctgggtcaac caccgggtgc agtgaaggtg
    12841 gttccgccaa gccgctttga atggactcct gcaattccgt cttgtgggtt ttgtgaattc
    12901 tgctttgccg gcgcatgtag agcaccagca ccacgccaaa caccaaggca tagagataga
    12961 ggtaattcat cgcagcacgc gcacactacg gtttgacagt ttccttgatt tcaagcttta
    13021 cccctgtcgc gcccactttg acggcaccag tttgccccga caaatcgccc ggctggggca
    13081 tggcattgcc gctcttgctg acacgggcac ccacgatcac ctggggcgaa ccggaaaggg
    13141 cattcgcagg cgtcatggcc atgctgtcat ccagggtgaa ttgcagcggc aagtctttga
    13201 cttgcttgcg caatatcgcc agaggcattt tggagccagc agccgcgcgg gcaaaaacaa
    13261 acacggtgtc ttccggctgt gcgagctttc gcagttcggg tgccagcgtg accgtcccac
    13321 tgacagtggc ctccgcaggt acagcgtttt tgccagaatc cagactgggc gcagccggta
    13381 gcccggctag gttacgcgct tctgccaggc ccggctggat ttgctgcacc agggtgttgt
    13441 ccgctgcacc gaattgaacg accttctccc agttcctgac tgcgccagca tagtctttct
    13501 tgttgaaagc atgggttccc gccagtgaaa gtgccttgag gttgcgggga tccagcttca
    13561 gggcacgctc cacctgcctc atgggctcgc catccagagc gcggttgttt ttcaccgcca
    13621 gggagtcggc atagtctgcc agcagcgtgg catcgtcctt gcgcaatcca acagcctttt
    13681 cgtaggcctt gagggcttca gggtgttttc caaggacact gtaggacctg gccagcatgg
    13741 cccatccctc tgcgtcatcg ggctgctcct tcatccgcgc agccagcttc tcggtcatgg
    13801 cagcgatctg ctctgaatcg gtcgcatgtg ctgcgccgga tgtcccctct gtattgcccg
    13861 caccaggcgc ttgcgccatt ggctggctgg tcaaggaagg tgagcccttc caccaatagc
    13921 cagcagcagc aatagccaca atgcccgcga ccagtgccgc caccaacagg ccggacggcc
    13981 tggctgccgt ccttgatgcg tcgtgtgcca aagtctgtgc gacgctttcc accggtacta
    14041 ccggcacggt cgcacccgag agcaccaggt caagcaaccg gcgttccagc gtggtctttc
    14101 cctcttcgta ctgtggctgg gaaagcacac cggattcgtg cagctctttg agttgcgcaa
    14161 gttggcgctt gatggcgtcg atggggttgt ccatgaatac agtctcgcaa aaaatatcag
    14221 gagagatgcg ggtcttgccc cgcatcgggg gattcaggat caaactgatc ctgcggcatg
    14281 cggctgcgtc ggcgaagcac gataaccaga atggccaagc cagccaccac catgacccca
    14341 gggccaatcc atagcagcgc agtggtggct ttgaagggcg gccggtacag cacaaaatca
    14401 ccatagcggg cagtcatata cgcaatgatc tcctgctccg actggccctt ttgcagcatt
    14461 tcccgaacct gctgacgcaa atccaccgcc agtccggcgt gtgaatcagc gatggtctgg
    14521 ttctggcaca ccaggcaacg cagttcactg gagatgcgca tcatgcgggc ctccagcgcc
    14581 gggtcggcag acgccggtgc cgcttcgttc gcaaaagcaa agccgaccca gaacggcagg
    14641 ctcatccaca gaatccattt agccattgag ttgcctcacc aggggttcga tttgtgtacg
    14701 gatcacttcc ggcgtgagtg gaccgatctg tttgaaccgg atgatgccct cacgatccac
    14761 gatgaaggtt tccggcacac catagacccc ccagtcaatg cccacccggc cgtcgttgtc
    14821 aaacagcgag gcatcgtaag gattgccaaa attggcaagc cagcgcaaac ctgccgccct
    14881 gtcatccttg taattcagtc catagatggg caatagcttc ttgcgcgaga actccaccag
    14941 caagggatgc tcttctcgac aggcaacgca ccaggaagcc cagacattga gcatccacac
    15001 cttgcccagc agatcttcgc gacggatggt cttctcggga ttgtccaact tggtcagaga
    15061 aaaaccgggt gcggacttgc caatcagtgg ggaaggaacc gttttgggat ccaggcccaa
    15121 ccccctcgcc aaaaaccaca ccaaccccaa gaatacaaac agcggaatca ggaaacgaag
    15181 gtacttcatg ccgttgccac cttctgcttt ctgcggtagc gtcggtcgga aactgccatc
    15241 agtccaccca aagccatcaa catgcaacct ccccatatcc agtcaataaa gggttttaca
    15301 tagacccgca cgatccaggc gccatcgtct acctgctcac ccatggaaac atacagatca
    15361 cgggtcagac ccgtactgat ggcagcttcc gtcatggggt tctgctgaac ccgatagacc
    15421 cgcttttcgg gctgaagctc ggcaatacgg tctctaccgc gtgtgatctc catttgccct
    15481 tgcgctgcca cgtaattcgg accttttatc tcccgaactc ccttgaaggt gaaggtatat
    15541 ccacggaccg tcgtgctgtc acccacgttc atcttgacat cactttcgac ctcataggtt
    15601 ttgaccatca cgacaccgaa agtaaacacc gccaccccaa tgtgggccac catcatgccg
    15661 atcaaggcac gtggcaactg ccgccacttg cggcgaattt ccgtcagccc cctgccctcc
    15721 ggctggatac gctcccacag atcggtggca acagaggtga atatccagta agccatcaac
    15781 aggccacaag ttgccagaag gtggatttcg ccggcaagca ggcctgtgga aatcgctgct
    15841 acgacagtca cgcccagtgc ccagcgcagg cgcttggcca gtgccggaac atctgcctcc
    15901 ttccagcgcg ccataggacc tatgcccatc aagaaaaccg tgggtgccat caggggcatg
    15961 aatacattgt caaagtaagg cgggccgacg gaaatctttc ccatgcccag ggcgtccagc
    16021 agcaacggat acagggtacc cagcaatacc gcaccggcag cgaccaggaa caacacgttg
    16081 ttggccaaaa gcgcggtctc cttcgacatc agcgcaaagc gggcacccag gcccacggtg
    16141 ggtgcccgcc aggcatacag tgcgaacgac gccccaacca cgatagtcag aaaagccagg
    16201 atgaacagac cccggcgcgg atctgttgca aaagcgtgca cggacgacaa cacgcccgaa
    16261 cgcacaagaa acgtacccag cagcgagaac gagaatgcga ggatgg
//
LOCUS       DAOSJM010000004        15809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_3884_length_15809_cov_4.940079, whole genome shotgun sequence.
ACCESSION   DAOSJM010000004 DAOSJM010000000
VERSION     DAOSJM010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15809
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3884_length_15809_cov_4.940079"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKX82_00325"
     CDS             complement(<1..370)
                     /locus_tag="PKX82_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOF50716.1"
                     /translation="MGKRIAFIGGGNMASAIIGGLLRQGFAPGQIDVVEPWAEARERL
                     QKDYGVVAQAEAGAFLAAADMVVWAVKPQTFKEAATQVRAHNTGALHLSVAAGIRSDS
                     IAQWLGTERIIRTMPNTPALI"
     gene            complement(439..1929)
                     /gene="glpK"
                     /locus_tag="PKX82_00330"
     CDS             complement(439..1929)
                     /gene="glpK"
                     /locus_tag="PKX82_00330"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOF50717.1"
                     /translation="MTYLLALDQGTSSSRSIVFDPSGHTVAMAQKELTQIYPQPGWVE
                     HDALEIWRIQLATAREALAKAGITAAQVKAVGITNQRETTVVWNRKTGQPIHHAIVWQ
                     DRRAEPTCVELRARGLADTIREKTGLLVDAYFSGTKLKWILDHVPDARAQAAHGELAF
                     GTIDSWLIWQLTEGREHVTDVSNASRTMLLNVHTNQWDEDLLAALDIPASLMPRVQAS
                     ASLFGEVAPTLLGQAIPIGGVAGDQQSALFGQACFKAGMVKNTYGTGCFMLMHTGDNF
                     QTSHNGLITTSAAQPTATPEFALEGSVFVGGAVVQWLRDGLHAIQGSGEVQSLAQSVP
                     DSGGVIVVPAFTGLGAPYWQPDARGSITGLTRGSTIAHIARAALESIAFQSAALLQAM
                     SRDAISAGGAQVSELRVDGGACVNDLLMQFQADLLGIPVVRPAITETTALGAAYLAGL
                     TTGVYQSTDELTDLWKAERRFLPTLPSERAQELMERWEHAVRQTVL"
     gene            complement(1948..2715)
                     /locus_tag="PKX82_00335"
     CDS             complement(1948..2715)
                     /locus_tag="PKX82_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOF50718.1"
                     /translation="MTPNPRQLTLLSVVQAQGSVTVEQLAETLGVTLQTVRRDVQRLA
                     DEGLLARFHGGVRVPSSTVENIAHQQRESLNAEGKSRIARKVAAAVPNDCSLILNIGT
                     TTEAIARALLHHSGLRVITNNLNVASILSTNPRCEVIVVGGVVRGRDQGIVGEAAVDF
                     IRQFKVDLALIGISGIESDGTLRDYDYREVKVAQTIISHAREVWLAADISKFKRPAMV
                     EVATLSQIDRLFTDAAPPDPFPALLADALVRLDVALS"
     gene            2877..3950
                     /locus_tag="PKX82_00340"
     CDS             2877..3950
                     /locus_tag="PKX82_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF50719.1"
                     /translation="MQLNLEGISKKVGPQTWLYDMSLAPRSGAVTVLLGATQAGKTSL
                     MRIMAGLDTPTAGAVRVNGVDVTGMPVRQRNVAMVYQQFINYPSLKVRDNIASPLKLR
                     GDRNVDQRVRELAEKLHIEMFLDRYPAELSGGQQQRVALARALAKGAPLMLLDEPLVN
                     LDYKLREELRDELTALFAAGSSTVIYATTEPGEALLLGGYTAVLDAGELLQYGPTSEV
                     FHAPQSIRVARAFSDPPMNLLVASAAATGVQLAHGPHIELTLPPTRSATLTVGMRASA
                     LRVRSQEGDVALVGTVELAEISGTDTFVHFETPLGELVAQLTGVHHFDLGQPCTFFFN
                     PAQVYVFDAQGMLLLAPVRGKGR"
     gene            3952..5028
                     /locus_tag="PKX82_00345"
     CDS             3952..5028
                     /locus_tag="PKX82_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF50720.1"
                     /translation="MARIELDLAHAYRPNPQQESDYALLPLKMTFEDGGAYALLGPSG
                     CGKTTLLNIMSGLVAPSQGTVRFDGKDVTRATPQERNIAQVFQFPVIYDTMTVAENLA
                     FPLRNRKMPADQIKKRVGVIAEMLEMSGQLNQRAAGLAADAKQKISLGRGLVRPDVSA
                     VLFDEPLTVIDPHLKWQLRRKLKQIHHELKLTLIYVTHDQVEALTFADQVVVMTRGRA
                     VQVGTAADLFERPSHTFVGHFIGSPGMNFLPGRNADGDFHTVGAVVALPHTRPLANGD
                     FTLGVRPEYVGLAEAHAPGALSMTVTQVQDVGTHVILSATREGRTVKARLAPDATQLT
                     PGDSVWLQVMGEHTCFYQNEEITA"
     gene            5025..5918
                     /locus_tag="PKX82_00350"
     CDS             5025..5918
                     /locus_tag="PKX82_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOF50721.1"
                     /translation="MSTTTKPVNQKAWFLILPVLLCVAFSAILPLMVVVNYSVQDIIS
                     PERRVFVGTEWFAAIMRDEELHGALLRQITYSFAVLAVELPLGILLALSMPSQGWKSS
                     AALVIVSLSLLIPWNVVGTIWQIFGRTDIGLMGAALQGMGLEYSYTGNAMHAWLTVLL
                     MDVWHWTPLVALLGYAGLRSIPDAYYQAASIDGASKLDVFRYVQLPKMRGVLMIAVLL
                     RFMDSFMIYTEPFVLTGGGPGNSTTFLSQFLTQKAVGQFDLGPAAAFSLIYFLIILLM
                     CFILYNWMQRVGTQAKEGGHE"
     gene            5911..6723
                     /locus_tag="PKX82_00355"
     CDS             5911..6723
                     /locus_tag="PKX82_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOF50722.1"
                     /translation="MSKPKFQKRSLFMLAYVIFALLPIYWMVNMSFKTNNEILAGFTL
                     FPDHFTWDNYRTILTDPAWYSGYINSLIYVGINTVISLTVALPAAYAFSRYSFLGDKH
                     VFFWLLTNRMTPPAVFLLPFFQLYTSVGLMDTHIAVALVHLLFNVPLAVWILEGFMSG
                     IPREIDETAYIDGYSFPRFFIRIYIPLIKAGVGVAAFFCFMFSWVELLLARTLTSVNA
                     KPIAATMTRTVSASGMDWATLAAAGVLTIVPGAIVIWFVRNYIAKGFAMGRV"
     gene            6736..7044
                     /locus_tag="PKX82_00360"
     CDS             6736..7044
                     /locus_tag="PKX82_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HOF50723.1"
                     /translation="MFDWMAWTTPVAVFFCCIALMLVGMTVWEIKSPTIARKGFLPME
                     TTRGDRLFVGLLSAAYVNLAFVGISGRLGEWLGLESEPSIWISFVLSMFLLGLVMRKG
                     "
     gene            7133..8747
                     /locus_tag="PKX82_00365"
                     /pseudo
     CDS             7133..8747
                     /locus_tag="PKX82_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699976.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     assembly_gap    8748..8757
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8982..10598
                     /locus_tag="PKX82_00370"
     CDS             8982..10598
                     /locus_tag="PKX82_00370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOF50724.1"
                     /translation="MANVASPLPTLRADLITRLSQDVSYDIAIVGGGATGLGVALDAA
                     ARGLKVVLVESHDFAKGTSSRATKLVHGGVRYLAQGNISLVREALHERTTLLNNAPHL
                     AQPLAFVVPSYKWWETPYYGAGLSAYDLLAGRAGLGKTEFLGRQATLGALPKVLGAGL
                     KGGVRYWDGQFNDARLALALARTAAQRGALLVNYCRANDLTFVDGQISGLVCEDTLTG
                     RTYVVRSRCVVNATGVWVDALREKDGQANAGDGRRPTQPMVAPSQGVHIVVDREFLGG
                     DTALMIPKTADGRVLFAVPWLGKVILGTTDTPRHDLPREPMPFQEEVDFILNESARYL
                     QRAPTVADIRSIWVGLRPLVKPQDDDGENTKGLSREHTVLVSRSGLVTVTGGKWTTYR
                     AMAEDVVQKCMNKGLIPGDFKEVTANIRLTGVVSPGDVATSICQPEGLHSYGSEQAAV
                     EAMPGAQHWLYPSLSEAMVRFAARYEYAVTVEDVLARRARLLFLDAALAARMAPEVGK
                     ILEQETGLPAQTEEFLVLSQQYLHPVIKKC"
     gene            10772..11140
                     /gene="rplN"
                     /locus_tag="PKX82_00375"
     CDS             10772..11140
                     /gene="rplN"
                     /locus_tag="PKX82_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOF50725.1"
                     /translation="MIQTESRLEVADNTGAKSVLCIKVLGGSKRRYASVGDIIKVSIK
                     EAAPRGRVKKGEIYSAVVVRTAKGIRRGDGSLVKFDGNAAVLLNAKLEPIGTRIFGPV
                     TRELRTEKFMKIVSLAPEVL"
     gene            11150..11470
                     /gene="rplX"
                     /locus_tag="PKX82_00380"
     CDS             11150..11470
                     /gene="rplX"
                     /locus_tag="PKX82_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOF50726.1"
                     /translation="MNKIRKGDEVIVIAGRDKGKRGTISLRKDDSHVVVEGINLVKKH
                     TKPNPMKGTTGGIVEKTMPIHQSNVAIFNAATGKADRVGIKVLADGKRVRVYKSSGEE
                     IKVA"
     gene            11473..12012
                     /gene="rplE"
                     /locus_tag="PKX82_00385"
     CDS             11473..12012
                     /gene="rplE"
                     /locus_tag="PKX82_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOF50727.1"
                     /translation="MARLQQHYREKVAPELTAKFGYTSPMQVPRLTKITLNMGVSEAV
                     ADKKVMDNAVGDLTKIAGQKPVVTKSKKAIAGFKIRENQAIGCMVTLRGVQMYEFLDR
                     FVTVALPRVRDFRGISGRAFDGRGNYNIGVKEQIIFPEIEYDKVDALRGLNISITTTA
                     KTDEECKALLAGFRFPFKN"
     gene            12021..12326
                     /gene="rpsN"
                     /locus_tag="PKX82_00390"
     CDS             12021..12326
                     /gene="rpsN"
                     /locus_tag="PKX82_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOF50728.1"
                     /translation="MAKMALIERELKRDKLAAKYAKKYAELKAIAGDAKRSDEERAAA
                     RLGLQKLPRNANPTRQRNRCSITGRPRGTFQHFGLGRAKIREMAFAGEIPGIVKASW"
     gene            12345..12740
                     /gene="rpsH"
                     /locus_tag="PKX82_00395"
     CDS             12345..12740
                     /gene="rpsH"
                     /locus_tag="PKX82_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOF50729.1"
                     /translation="MSMSDPIADLLTRIRNAQMVAKTTVLVPSSKVKVAIAQVLKEEG
                     YIDGFKVSTEGGKAELEIALKYYAGKPVIERIERVSRPGLRVYRGSDAIPQVQNGLGV
                     AIVTTPKGVMTDRKARATGVGGEVLCYVA"
     gene            12764..13297
                     /gene="rplF"
                     /locus_tag="PKX82_00400"
     CDS             12764..13297
                     /gene="rplF"
                     /locus_tag="PKX82_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOF50730.1"
                     /translation="MSRVGKLPVNVPAGVDVSIKEDQISVKGTGGTLQLAQNSLVKIA
                     NDAGKLSFQPANDSREADAMTGTMRQLVNNMVVGVTKGFEKKLSLIGVGYKAQATGAK
                     LNLAVGYSHPVNVDMPAGITVATPTPTEILIKGADRQRVGQIAAEIRAIRPPEPYKGK
                     GIRYADEKITIKETKKK"
     gene            13309..13674
                     /gene="rplR"
                     /locus_tag="PKX82_00405"
     CDS             13309..13674
                     /gene="rplR"
                     /locus_tag="PKX82_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOF50731.1"
                     /translation="MLTKKEQRLRRARQTRIRIATQGVARLTVTRTNLHIYASVISGD
                     GGKVLAAASTAEADVRKSLGGSGKGGNVAAAQIIGKRVAEKAKAAGVEKVAFDRSGFA
                     YHGRVKALADAAREAGLQF"
     gene            13692..14216
                     /gene="rpsE"
                     /locus_tag="PKX82_00410"
     CDS             13692..14216
                     /gene="rpsE"
                     /locus_tag="PKX82_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOF50732.1"
                     /translation="MAKVQAKMQGKAEGPEDGLREKMIAINRVTKVVKGGRILGFAAL
                     TVVGDGDGRIGMGKGKSKEVPAAVQKAMEEARRNMLKVSLKNGTIHHKVMGHHGAASV
                     MMAPAPKGTGIIAGGPMRAVFEVVGITDIVAKSHGSTNPYNMVRATLDALSVSTTPAE
                     VAAKRGKSIEEIFA"
     gene            14232..14414
                     /gene="rpmD"
                     /locus_tag="PKX82_00415"
     CDS             14232..14414
                     /gene="rpmD"
                     /locus_tag="PKX82_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOF50733.1"
                     /translation="MTTQQTVKVQLVRSPIGTKESHRATVRGLGLRKMGSVSELQDTP
                     AVRGMINKISYLVKVL"
     gene            14425..14856
                     /gene="rplO"
                     /locus_tag="PKX82_00420"
     CDS             14425..14856
                     /gene="rplO"
                     /locus_tag="PKX82_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOF50734.1"
                     /translation="MELNGIKPADGAKHAKRRVGRGIGSGLGKTAGRGHKGQKSRSGG
                     YHKVGFEGGQMPMHRRLPKRGFKSHLLQFNAEVTLTALDALGVTEADLLTLKQLGAVA
                     EKAKVVKVINTGAITKAVKLTGIGATAGAKAAIEAAGGSVA"
     gene            14877..>15809
                     /gene="secY"
                     /locus_tag="PKX82_00425"
     CDS             14877..>15809
                     /gene="secY"
                     /locus_tag="PKX82_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOF50735.1"
                     /translation="MATNAAQIANSGKFGDLRRRLVFLLLALVVYRVGAHIPVPGIDP
                     AQLQQLFKGQQGGILSLFNMFSGGALSRFTVFALGIMPYISASIIMQLLTYVLPTFEQ
                     LKKEGEAGRRKITQYTRYGTLGLALFQSLSIAMALESSAGLVITPGFGFRLTAVVTLT
                     AGTMFLMWLGEQITERGLGNGISILIFGGIAAGLPSAIGGLLELVRTGAMSIIIALLI
                     VVLVALVTYFVVFVERGQRKILVNYARRQVGNKVYGGQSSHLPLKLNMAGVIPPIFAS
                     SIILLPATIANWFSAGDSMRWLKDIASTLSPGQPV"
BASE COUNT         3235 a         4329 c         4903 g         3332 t
ORIGIN      
        1 caatcagcgc aggggtgttg ggcatggtgc ggatgatccg ctcggttccc agccactggg
       61 caatgctgtc actgcggatg ccggcggcta cgctcaggtg cagggcgccg gtgttgtggg
      121 cgcgcacctg ggtagctgcc tccttgaagg tttggggctt gaccgcccac accaccatgt
      181 cggcggcagc caggaacgcg cctgcttcgg cctgggcgac gacgccgtaa tccttctgca
      241 ggcgttcgcg ggcctcggcc cagggttcaa ccacgtcgat ctggccgggc gcaaatccct
      301 ggcggagcag gccgccgatg atggcgctgg ccatattgcc gccgccgatg aacgcaatgc
      361 gtttgcccat ggtcattctt tcaggttctg tctgtggagc gtcagagtct atccaaagca
      421 aggggcgcac tctggcgcct agagcactgt ctggcgtacc gcgtgctccc agcgctccat
      481 cagctcctgg gcgcgctcgc tgggcagcgt cggcaggaaa cggcgctcgg ccttccacag
      541 gtcggtcagc tcatccgtgc tctggtaaac accggtggtc agaccagcca ggtaggcggc
      601 acccagggcg gtggtctcgg tgatggcggg gcgcacgacc ggaatgccca gcaggtcggc
      661 ctggaactgc atcagcaggt cgttcacgca ggcgccgccg tccacccgca gctccgacac
      721 ctgtgccccc cctgcgctga tggcgtcccg actcatggcc tgcaacaggg cggcactctg
      781 gaaggcgatg ctctccagcg ccgcgcgcgc aatgtgggca atggtgctgc cccgggtcag
      841 gccggtgatg gagccgcgtg cgtcgggttg ccagtagggc gcacccaggc cggtgaaagc
      901 gggcaccacg atgacgccgc ccgagtccgg cacactttgc gccaggcttt gcacttcacc
      961 gctgccctga atggcgtgca ggccgtcgcg cagccattgc accacggcgc cacccacaaa
     1021 gacgctgcct tcgagtgcga attcgggcgt ggcggttggc tgcgccgcac tggtggtgat
     1081 gagtccgttg tgcgaggtct ggaagttgtc accggtgtgc atcagcatga agcatccggt
     1141 gccataggtg ttcttgacca tgccggcctt gaagcaggcc tggccaaaca gggcgctctg
     1201 ctggtcgcct gccacgccgc caatgggaat ggcctggccc agcagagtgg gtgccacttc
     1261 accgaacagc gaggcggagg cctgcacccg gggcatcagg ctggcgggga tgtccagcgc
     1321 cgccagcagg tcttcgtccc actggttggt gtgcacattg aggagcatgg tgcgtgaggc
     1381 attgctgaca tcggtgacgt gctcccggcc ttcggtgagc tgccagatca gccagctatc
     1441 gatggtgcca aaggccagct ctccgtgggc tgcctgggcg cgggcgtctg gtacgtggtc
     1501 cagaatccat ttgagtttgg tgcctgaaaa ataggcgtcc accagcaggc cggttttttc
     1561 gcggatggta tcggcaaggc cgcgcgcgcg cagctctaca caggtgggct cggcccggcg
     1621 gtcctgccag acgatggcat ggtgaatggg ctggccggtc ttgcggttcc atacgacagt
     1681 ggtttcacgc tggttggtga tgcctacagc cttcacctgg gccgcagtga tgccggcctt
     1741 ggccagcgcc tcgcgcgccg tggcgagctg gatgcgccag atttccaggg catcgtgctc
     1801 cacccagcca ggctgggggt agatctgggt cagttccttt tgcgccatcg ccacggtgtg
     1861 gccactgggg tcgaacacga tgctgcggga gctggaggtg ccttggtcga gggcaagcag
     1921 gtaggtcatg gcaatggagt atccaaatca ggagagtgcg acatccagcc gcaccagggc
     1981 atcagccagc agtgcgggga acgggtcggg tggtgcagca tcggtgaaca ggcggtcgat
     2041 ctgcgatagc gtggccacct cgaccatggc cgggcgcttg aacttgctga tgtcggcggc
     2101 cagccagact tcccgcgcgt gcgaaatgat ggtttgggcc actttgactt cgcgatagtc
     2161 atagtcgcgc agcgtgccgt cggactcgat gcccgaaatg ccaatcaggg cgagatccac
     2221 cttgaattgg cgaataaagt ccacggccgc ctcgcccacg atgccctggt cacgcccgcg
     2281 caccacaccg cccaccacga tgacttcgca gcgggggttg gtgctgagga tgctggccac
     2341 attcaggttg ttggtaatga cgcgcaggcc gctgtggtgc agcagggcgc gcgcaatggc
     2401 ctcggtggtg gtgccaatgt tgaggatcag tgagcagtcg ttggggactg cagcagctac
     2461 cttgcgcgca atgcgcgact tgccctcggc gttcaggctt tcgcgttgct ggtgggcgat
     2521 gttctctacg gtggagctgg gcacccggac gccgccatgg aagcgcgcca gcaggccctc
     2581 gtcggccaga cgctgcacat cacgccgcac ggtctgcagg gtgacgccca gggtctcggc
     2641 cagctgctcc accgtgacgg agccttgtgc ctggacgaca gagagcagag tcagttgtct
     2701 ggggtttggg gtcattgtgt gtttgtaccc tgaatctgca gggcggcttg actgtaaaac
     2761 gaatcaaaaa gaatatttat agggttagta ctgagataaa acgaatcaaa acgaacaata
     2821 attctcgaat tcgaaaatac tcacggggtc aaaaccctgc aagaaaaggt ggcgtgatgc
     2881 agttgaatct ggaaggcatc agcaagaaag tagggccgca gacctggctg tacgacatga
     2941 gcctcgcacc gcgcagcggc gctgtgacgg ttttgttggg cgcaacccag gcgggaaaaa
     3001 ccagcctgat gcgcatcatg gcaggactgg atacgcccac cgcaggagcg gtccgggtga
     3061 atggcgtgga tgtgactggc atgcctgtgc gccagcgcaa tgtggccatg gtttaccagc
     3121 agttcatcaa ctacccctca ctcaaggtgc gcgacaacat tgcctcgccg ctgaaactgc
     3181 gcggtgaccg gaatgtggac cagcgcgtgc gcgagttggc cgagaagctc cacattgaga
     3241 tgtttctgga ccgctatccc gcagaactgt cgggcggcca gcagcagcgc gttgcactgg
     3301 cccgtgcact ggccaagggc gcaccgctga tgctactgga tgagccgctg gtgaacctgg
     3361 actacaagct gcgcgaagaa ttgcgcgacg agttgaccgc actgttcgct gcggggagct
     3421 ctacggtgat ttatgccacc accgaacccg gcgaagccct gctgctgggt ggctataccg
     3481 cggtgttgga tgccggtgag ctgctgcagt acggacctac atctgaagtg tttcatgcgc
     3541 cccagtccat ccgtgtagcg cgtgctttca gtgacccgcc catgaacctg ctagttgcca
     3601 gtgcagcagc cacgggtgtg caactggccc atggaccgca catcgagctg accctgccgc
     3661 ccaccaggtc ggccacgctg acggtgggca tgcgtgccag cgcgctgcgg gtgcgttccc
     3721 aagagggcga tgttgcgctg gtgggcacgg tggagctggc cgagatttcg ggcaccgaca
     3781 cgtttgtgca cttcgaaacg ccattagggg agctagtggc tcagctgacc ggggtgcacc
     3841 acttcgatct gggacaaccc tgcactttct ttttcaatcc tgcgcaggtc tatgtgttcg
     3901 acgcgcaggg catgttgctg ctggccccgg tgcgcggcaa aggacgctga tatggcacgc
     3961 atagaacttg atctggccca cgcctaccgg ccgaacccgc agcaggaaag cgactacgcc
     4021 ctgctgcctc tgaagatgac ctttgaagat ggtggcgcct acgcgttgct gggcccatcg
     4081 gggtgtggca agaccacatt actcaacatc atgtcggggt tggtagcacc ttcgcagggc
     4141 acagtgcgct ttgacggcaa ggatgtgaca cgtgcgacac cgcaggagcg caatattgcg
     4201 caggtattcc agtttccggt gatctacgac accatgacgg tggccgagaa cctggccttc
     4261 cccctgcgca accgcaagat gcccgccgac cagatcaaga aacgcgtggg cgtcatcgcg
     4321 gagatgctgg agatgagcgg ccagctcaac cagcgtgccg ccggtctggc agctgacgcc
     4381 aaacagaaga tttctctggg tcgggggctg gttcggcctg atgtgtcggc ggtgctgttt
     4441 gacgagccgc tcaccgtgat tgatccgcac ctgaaatggc agttgcgtcg caagctcaag
     4501 cagatccacc acgaactcaa gctcacgctg atttatgtca cccacgacca ggtggaggcg
     4561 ttgacattcg ctgaccaggt ggtggtgatg acacgggggc gggcggtgca ggtggggacc
     4621 gcggctgatt tgtttgagcg gcccagccac acgtttgtgg gccatttcat tggctcgcca
     4681 ggcatgaatt ttttgcctgg ccgcaatgcg gatggtgact ttcacacagt gggtgcagtg
     4741 gtggcgctgc cgcacacgcg accgcttgcc aatggcgact tcacgttggg cgtgcgtccc
     4801 gagtacgtgg gtctggccga agcccatgcg ccgggcgcac tgtccatgac cgtgacccaa
     4861 gtgcaggatg tgggaacgca tgtaatcctc agcgccacac gcgaaggacg cacagtcaag
     4921 gcgcggctgg ctcccgatgc cacacagcta accccgggag acagcgtgtg gctgcaggtc
     4981 atgggcgaac acacctgctt ctaccagaac gaggagatca cggcatgagc acgacgacca
     5041 aaccggtcaa ccagaaagcc tggttcctga tcctccctgt gctgctgtgc gtggcgtttt
     5101 cggccatcct gcctttgatg gtggtggtga attactcggt gcaggacatc atctctcccg
     5161 agcgcagggt gtttgtgggc acggagtggt tcgctgccat catgcgcgac gaggaactgc
     5221 atggcgcgct cttgcgccag atcacctact cgtttgcagt gctggctgtg gagttgccac
     5281 tggggatatt actcgcactg tccatgccct cccagggctg gaagtcttcg gcggcgttgg
     5341 tgattgtgtc gctgtccctt ctgattccct ggaacgtagt gggcaccatc tggcagattt
     5401 ttggacgcac cgatatcggt ttgatggggg ctgcgctgca aggcatgggc ctggagtaca
     5461 gctataccgg aaatgcgatg cacgcctggc tgacggtgct gctgatggac gtgtggcact
     5521 ggacgccgct ggtggcactg ctgggctatg ccgggctgcg ctctattccc gatgcttact
     5581 accaggcggc aagtatcgat ggtgccagca agcttgatgt gtttcgctat gtgcagctgc
     5641 caaaaatgcg cggtgtgctg atgattgcgg tgttgttgcg cttcatggac agcttcatga
     5701 tttacaccga gccttttgtg ctgaccggcg gtggtccagg caactccacc accttcctgt
     5761 cgcagtttct cacgcaaaag gcggtcggcc agtttgacct gggacctgcg gccgcgttct
     5821 cgctgatcta tttcctgatc attttgttga tgtgttttat cttgtacaac tggatgcagc
     5881 gcgtcggcac ccaggctaag gagggtggcc atgagtaagc ccaaattcca gaagcgttcg
     5941 ctgttcatgc tggcgtacgt catatttgcg ctgttgccca tttactggat ggtcaacatg
     6001 tcgttcaaga cgaacaacga aatcctggca ggtttcacgc tgttcccgga ccatttcacc
     6061 tgggataact acaggaccat cctgaccgat ccggcctggt actcgggcta catcaacagc
     6121 ctgatctatg tgggcatcaa cacggtcatt tccttgaccg ttgccctgcc cgcagcctat
     6181 gcgttttcgc gctacagctt tctgggtgac aagcatgtgt tcttttggct gctgaccaac
     6241 cgcatgacgc cacccgccgt gttcctgctg cccttctttc agctctacac ctcggtgggc
     6301 ctgatggaca cccacattgc ggtggcgctg gtgcacctgt tgttcaacgt gccgctagcg
     6361 gtgtggatcc tggaaggctt catgagcggc atcccgcgcg agattgacga gaccgcctac
     6421 atcgacggct attcattccc gcgcttcttc atccgcattt atatcccgtt gatcaaggcc
     6481 ggtgtgggcg tggccgcgtt cttttgcttc atgttcagct gggtggagtt gctgctggct
     6541 cgcacgctga ccagtgtgaa tgccaagccg attgccgcca ccatgacccg caccgtttct
     6601 gcatcgggca tggactgggc gacgctggct gccgcgggtg tcctgaccat cgtaccgggt
     6661 gccatcgtga tctggtttgt ccgcaattac atcgcgaagg gtttcgcgat gggacgtgtt
     6721 tgagaggaat gcacgatgtt tgattggatg gcttggacca cgccggtggc agtatttttt
     6781 tgctgtatcg cgctgatgct ggtgggcatg acggtgtggg aaatcaaatc ccccaccatt
     6841 gcgcgcaaag gctttctgcc tatggaaacg acccgcgggg atcgtctttt tgtggggctg
     6901 ctcagcgccg cctatgtgaa tctggcattc gtgggcatca gtgggcgact gggcgagtgg
     6961 ttggggcttg agtcggagcc ctcgatctgg atcagctttg tgctctccat gtttctcctg
     7021 ggactggtga tgcgtaaggg ctgacaagca acgattttca aggttcggct tataccccct
     7081 ggggccgcgc cattctttca aacaggggtt catcagaccg aggagattga caatgaagtt
     7141 gagatattcc gtactggccg tcgccatggc ttgcgctgtg ggtacccaag catgggcaga
     7201 tgaagcggcg gctaaaaaat ggattgacag cgaattccag ccctccactt tgagcaaggc
     7261 ccaacaggct gccgaaatga agtggtttat ggacgcggcc aaaaaactgc agggcaaggg
     7321 tatcaaggaa atttccgtgg tgtccgagac catcaccacc cacgaatacg aatccaaaac
     7381 actggccaag gcttttgagg aaatcacggg gatcaaggtc aagcatgatc tgattggcga
     7441 aggtgatgta gtcgaaaaac tgcaaacttc catgcagtcc ggcaagtcca tctacgacgg
     7501 ctggattact gactccgacc tgatcggtac ccactaccgc tacggcaaga tcatgaatct
     7561 gaccgactac atggccggtg cgggcaaaga gtggaccaac cccggtctgg acctcaagga
     7621 tttcatcgga accagtttca cctccggtcc tgacaagaag ttgtatcagt tgcccgatca
     7681 gcagttcgcc aacctgtact ggttccgcgc cgacctgttc gaaaagcccg agttgaaggc
     7741 caaattcaaa gccaagtatg gctatgacct gggcgtgccc ttgaactgga gcgcttacga
     7801 agacatcgct gcgttcttca gtgaagacgt gaagaccatc gacggcaagc cgatttatgg
     7861 tcacatggat tacggcaaga aagatccgtc gttgggctgg cgcttcaccg acgcgtggct
     7921 gtccatggcg ggtacagccg acgtaggcat tcccaatggc aagccagtgg acgagtgggg
     7981 tattcgtgcg tctgccgacg ggtgcacacc gcagggggct tctgtgtccc gtggcggcgc
     8041 gaccaactcg cccgctgccg tgtatgcact gaccaagtac atcgactgga tgaagaaata
     8101 ctcgcccaag gaagccatcg gcatgacttt cggcgaagcc ggccctgtac ccgcccaagg
     8161 ccagatcgcc caacagatct tctggtacac cgcctttacc gcggacatga tcaagcccgg
     8221 tttgcccgtg gtgaatgcag acggcacgcc caagtggcgc atggctcccg gtcctaacgg
     8281 accgtactgg aaacaaggta tgcaaaacgg ctaccaggat gtgggctcat ggactttctt
     8341 caaggatcat gatgccaaca aggtcgctgc tgcctggctg tacgcccagt tcgtgactgc
     8401 caagaccact tcactcaaaa agaccgttgt cggtctgacg cccatccgcg agtccgacat
     8461 ccagtccaag gccatgacgg atatggcacc caagctcggt ggcctggtgg agttttatcg
     8521 cagtcctgcc cgtgtggcct ggactcccac cgggaccaat gtgcccgact atcccaagtt
     8581 ggcacagttg tggtggaaga acgtagccgt ggctgtcact ggcgaaaaga cgcctcaggc
     8641 tgcgatggat aacctggctg aagaaatgga tgcggtgatg ggccgcctgg agcgctctgg
     8701 gatggcggta ggtccgccta agctaaatgc aaaaagcagc cctgaaannn nnnnnnnaag
     8761 ggcgcaccgt ggaagaaact ggccaatgaa aagcctaagg gcgaaaccat tgcctatgac
     8821 actttgctca atgcatggaa gaacggcaag gttcgctaat gacgctcgag tgagcgactg
     8881 atcgaccccc atgcaggcat ttcttgcgtg ggggtgttca aaagaatctt tcggcggagc
     8941 tcagtattgg cgggtccgct gcgaatatgt agaaaacact tatggctaat gttgcgagtc
     9001 ccctcccgac cctgcgtgca gacctgatca cgcgcttgtc gcaggatgtc agctacgaca
     9061 tcgcgattgt gggcggcggt gccaccggtt tgggtgtggc cctggatgcc gcagcacggg
     9121 gactcaaggt ggttctggtg gagtcgcatg atttcgccaa aggcacgtcc tcccgggcga
     9181 ccaagctggt gcatggcggt gtgcgctacc tggcgcaggg aaatatcagc ctggtgcgcg
     9241 aggcactgca cgagcgcacc acgttattga acaacgcccc gcatctggcc cagccgctgg
     9301 cgtttgtggt gccttcctat aagtggtggg aaacgccgta ctacggggca ggactttcgg
     9361 cgtatgactt gctggcaggc agggcggggc tggggaagac cgaatttctc gggcgccagg
     9421 ccacgctggg ggctttgccc aaggtgttgg gggcgggcct caagggaggg gttcgctact
     9481 gggacggcca attcaatgac gcccggttgg ctctggcgtt ggcccgcacg gccgcgcagc
     9541 gcggcgcctt gttggtgaat tactgccggg ccaatgacct gacctttgtg gatggtcaga
     9601 tttccggact ggtctgcgaa gacaccctga ccggtcgtac ctatgtggtg cgttcacgct
     9661 gcgtggtcaa tgcgacaggg gtgtgggtgg atgcgttgcg cgaaaaggat ggccaggcca
     9721 acgcgggtga cggccgccga ccgacccagc ccatggttgc gccgagccag ggcgtgcata
     9781 ttgtggtgga ccgggagttc ctgggcgggg atacggcact gatgattccc aaaaccgccg
     9841 atggccgggt tctgtttgcc gtgccttggc tgggcaaagt aattctgggg actacggata
     9901 cgccccgcca cgatttgcca cgtgagccaa tgccatttca ggaagaggtg gacttcatat
     9961 tgaatgagtc ggcccgctac ctgcagcgtg cacccacggt agcggacatc cgcagcatct
    10021 gggtcggctt gcgtcctcta gtcaaaccac aggatgacga tggtgaaaac accaaggggc
    10081 tgagccggga gcacacggtg ctggtcagcc gcagtggact ggtgacggtc accggcggga
    10141 aatggaccac ctaccgcgct atggccgagg acgtagttca gaagtgcatg aacaaaggcc
    10201 tgataccggg cgatttcaaa gaagtgactg ccaacatcag gctgactggg gtggtatcac
    10261 cgggagatgt ggcaacctcg atttgccagc ccgagggttt gcattcctat ggatcagagc
    10321 aggctgccgt agaggcaatg cctggcgcgc aacactggtt gtaccccagc ctgagtgagg
    10381 ccatggtgcg ttttgccgcc cgctacgaat acgccgttac ggttgaggat gtactggcgc
    10441 ggcgcgcgcg gttgttgttt ctggatgcag ccctggccgc acggatggca ccggaagtgg
    10501 ggaaaattct ggagcaggag acggggttgc ccgcccaaac cgaagaattt ttggttttga
    10561 gccagcaata tttgcatccc gtcatcaaaa agtgttgact cggcaaaaat aggtaggcat
    10621 aatcgtgggc ttcgcttgaa aaagcagaag gttctgccca aatggatgtc gtgcaagtgg
    10681 tttgtgcggc ggacagcggg cccaagactg acctgttgtg gttctggcct taaggctggt
    10741 gctgcctccg gtgcaagttg ggaatattga aatgatccaa actgaatctc gactggaagt
    10801 tgccgacaac accggcgcga aatctgtcct ctgcatcaag gtgctgggcg ggtcgaagcg
    10861 tcgctatgca agcgttggcg acatcatcaa agttagcatc aaagaagccg ctccccgtgg
    10921 tcgcgtcaaa aaaggcgaga tctacagtgc tgtggtggtt cgtactgcca agggcatccg
    10981 ccgtggcgac ggttctttgg tgaaattcga cggcaacgca gcagtgttgc ttaacgccaa
    11041 attggagcct atcggcaccc gtatctttgg accagtgacg cgcgaactgc gcactgaaaa
    11101 gttcatgaag atcgtgtccc tggctcctga agttttgtaa ggacccgcca tgaacaagat
    11161 tcgcaaaggc gacgaagtca tcgttatcgc aggtcgcgat aagggtaagc gcggaacaat
    11221 ttccttgcgc aaagacgact cccatgtggt ggtcgagggc attaacctgg tgaagaagca
    11281 caccaagcca aaccccatga agggcacaac cggcggcatc gttgaaaaga ccatgcctat
    11341 tcaccagtcc aacgtggcaa ttttcaatgc tgcaacgggc aaggctgacc gtgttggtat
    11401 caaggtgctg gctgacggta agcgcgttcg cgtttacaag tcgagcggcg aagaaatcaa
    11461 ggtggcataa gcatggcacg tcttcaacaa cattaccgcg aaaaagtggc gcctgagctg
    11521 acagccaagt ttggctacac ctcgcccatg caggttcccc gtctgaccaa gatcaccctg
    11581 aacatgggtg tgagcgaggc ggttgctgac aagaaggtta tggacaacgc cgttggcgac
    11641 ctgaccaaga ttgctggcca gaagcctgtt gtaaccaagt ccaagaaggc tatcgctggt
    11701 ttcaagatcc gcgaaaacca ggcgattggt tgcatggtga ctctgcgtgg cgtgcaaatg
    11761 tacgaattcc tggatcgttt tgtgaccgtg gctctgcccc gtgttcgtga cttccgtggt
    11821 atttctggtc gcgcattcga tggccgtggt aactacaaca tcggcgtcaa agagcagatc
    11881 attttccctg aaattgaata tgacaaggtg gatgccttgc gcggcctcaa tatcagcatc
    11941 accacaacgg caaagaccga cgaagagtgc aaggcactgc tcgctggctt ccgtttcccg
    12001 ttcaagaact gaggtgacct gtggctaaaa tggctttaat cgagcgtgag ctcaaacgtg
    12061 acaagttggc tgccaagtac gcaaagaaat atgcggaact gaaggccatc gctggtgatg
    12121 caaagcgctc tgacgaagag cgcgctgcgg cccgtctggg tctgcaaaag ctgccccgca
    12181 atgcaaaccc cactcgccag cgtaaccgct gctccatcac gggtcgtccc cgtgggacgt
    12241 tccagcactt cggtctgggt cgtgcgaaga ttcgtgaaat ggcctttgct ggtgagatcc
    12301 ccggcatcgt caaggccagc tggtaagcga caggagaaac taatatgagt atgagtgatc
    12361 ccatcgccga cctgttgaca cgcatccgca atgcgcaaat ggtcgccaag acaacggttc
    12421 tggtgccttc ttccaaagtg aaggttgcga ttgcccaagt gttgaaagaa gaaggctata
    12481 tcgacggttt caaggtttcc accgaaggtg gcaaagctga actggaaatc gccctgaagt
    12541 actacgccgg caagcctgtg attgagcgca tcgagcgcgt cagccgccct ggtctgcgtg
    12601 tttaccgtgg cagcgatgcc atcccccagg tccagaacgg actgggtgtt gccatcgtga
    12661 cgacccccaa gggtgtgatg actgatcgca aggcgcgcgc taccggtgtc ggcggcgaag
    12721 ttctttgcta cgtcgcttaa cggcacatta ggagtaatta caaatgtccc gcgtaggcaa
    12781 acttcctgta aacgtccccg ccggtgtgga tgtgtccatc aaagaagacc agatcagtgt
    12841 caaaggcact ggcggcactc ttcagttggc acagaacagc ctggtgaaaa ttgcaaatga
    12901 tgctggcaag ctgagctttc agcctgccaa tgattcccgc gaagcagatg ccatgactgg
    12961 taccatgcgt cagctggtga acaacatggt ggtcggtgtg accaagggct tcgaaaagaa
    13021 gctgagcctg attggtgtgg gttacaaagc ccaggccact ggtgccaagt tgaatctggc
    13081 agttggttat tcgcatcccg tcaatgttga tatgcctgct ggcatcaccg tggcgacccc
    13141 cactcctaca gaaatcctga tcaagggcgc tgaccgtcag cgcgttggac agattgctgc
    13201 tgagattcgt gctattcgtc ctcctgagcc ttacaagggc aagggaatcc gctatgcgga
    13261 cgagaagatc acgatcaaag aaaccaagaa gaaataagga gctgcaacat gttgaccaaa
    13321 aaagagcaac gtcttcgcag agcccgtcaa acgcgcatcc gcattgcgac gcaaggcgtg
    13381 gcacgtttga ccgtgacccg taccaatctg cacatttacg ccagcgttat ctctggcgat
    13441 ggcggcaagg ttctggctgc agcatcgacc gccgaggcag atgttcgcaa gtccctgggt
    13501 ggttctggca agggcggcaa cgtcgctgct gcgcagatca ttggcaagcg cgtggctgag
    13561 aaggccaagg ccgctggtgt tgaaaaagtt gcgtttgacc gctccggttt tgcgtaccac
    13621 ggccgcgtta aagcgctggc agatgccgct cgtgaagctg gcttgcagtt ctaagcagat
    13681 cggaaattaa gatggctaaa gttcaagcaa aaatgcaagg taaggccgag ggtcctgagg
    13741 acggtctgcg cgagaagatg atcgcgatca atcgcgttac caaagtggtg aagggcggcc
    13801 gtattctcgg tttcgctgcc ttgaccgtgg tgggtgacgg cgatggtcgc atcggtatgg
    13861 gtaagggtaa atccaaggaa gtgccggctg ctgtgcagaa ggccatggaa gaagcccgtc
    13921 gcaacatgct gaaggtttct ctgaagaacg gcaccatcca ccacaaggtg atgggtcacc
    13981 acggcgcggc aagcgtcatg atggcaccgg ctcccaaagg taccggcatc attgcaggcg
    14041 gccctatgcg cgcagtgttc gaagtggtcg gaattaccga tatcgtggcc aagagccatg
    14101 gctcgaccaa cccctacaac atggttcgcg ccacgttgga tgcactgtcc gtgtctacca
    14161 ctccagctga agttgctgcc aagcgcggca agtcaattga agagatcttc gcttaaaacg
    14221 gagaattcac catgacaaca caacaaacag tcaaagtcca attggtgcgt agcccaattg
    14281 gtaccaaaga atcccaccgc gccactgtgc gtggtctggg acttcgcaaa atgggaagtg
    14341 tcagcgagtt gcaggatacg cctgcagtgc gcggcatgat caacaagatc agctatctgg
    14401 tcaaagtgct ctaagaggtt gatgatggaa ctcaatggca tcaagccagc agacggcgct
    14461 aaacacgcaa aacgtcgcgt tggtcgcggt attggatctg gcctgggtaa aaccgctggt
    14521 cgtggtcaca agggtcaaaa gtcgcgctcc ggtggctacc acaaggtagg tttcgaaggc
    14581 ggccagatgc ctatgcatcg tcgtctgccc aagcgcggct tcaagtcgca cttgctgcaa
    14641 ttcaatgctg aagtcactct gacagctctg gacgcgcttg gcgtgaccga agccgatttg
    14701 ctgacattga agcaactggg tgcagtcgct gagaaggcca aggtcgtgaa ggtgattaac
    14761 accggcgcga ttaccaaggc tgtcaagctc accggtattg gtgcgactgc tggtgcaaag
    14821 gctgctattg aagcggctgg tggcagcgtt gcctgatttg ctgacgagag atatttgtgg
    14881 caactaacgc agcgcaaata gcgaactccg ggaaattcgg cgacttgcgc cgtcgtctcg
    14941 tgtttttgct cctggcattg gtggtgtatc gggttggtgc acatattccg gtgccgggga
    15001 ttgaccctgc gcagcttcag caattgttca aaggccagca gggcggcata ttgagtcttt
    15061 tcaatatgtt ctctggtggt gcgctctcgc ggtttaccgt gtttgcgctg ggaatcatgc
    15121 cgtacatctc ggcatcgatc atcatgcaat tgctgactta tgtcctgcca accttcgagc
    15181 aattgaagaa ggaaggcgaa gctggccgtc gcaagatcac gcaatacacc cgctatggca
    15241 cgctgggatt ggccttgttc cagtccctga gtatcgcgat ggcattggag tcttctgccg
    15301 ggctggttat tacgcctggt ttcggattcc gccttacggc agtggtcaca ctcacggctg
    15361 gcaccatgtt cctgatgtgg ctgggtgagc aaatcactga gcgcggattg ggcaatggta
    15421 tttccatcct gatttttggt ggtatcgcag caggactccc cagtgcaatc ggtggactac
    15481 tggaactggt gcgcaccggt gccatgagta tcatcattgc ccttctgatc gttgtgttgg
    15541 tcgcactggt aacgtacttt gtcgtgttcg tggagcgcgg acagcgaaag attttggtga
    15601 actacgcgcg ccgtcaggtc ggcaacaagg tatatggagg tcagtcctcg catctgccct
    15661 tgaagctgaa catggctggt gtgattcctc caatttttgc gtcgtccatt attctgttgc
    15721 cggctacaat cgctaactgg ttcagtgctg gtgactccat gcgctggttg aaagatattg
    15781 caagcacgtt gagtcccggc cagccggtg
//
LOCUS       DAOSJM010000005        15784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_3890_length_15784_cov_4.163837, whole genome shotgun sequence.
ACCESSION   DAOSJM010000005 DAOSJM010000000
VERSION     DAOSJM010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15784
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3890_length_15784_cov_4.163837"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="PKX82_00430"
     CDS             complement(<1..839)
                     /locus_tag="PKX82_00430"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097657 - 3',5'-nucleotide bisphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-nucleoside bisphosphate phosphatase"
                     /protein_id="HOF50736.1"
                     /translation="MTTLLNADLHCHSVVSDGTLTPEALAARAHANGVELWALTDHDE
                     IGGQYRAAAAARELGMKYLTGVEVSVTFVGQTVHIVGLGFDADDERLANGLRQTRGGR
                     RERAMEMADGLAKVGIKGAFEGALQFVGNPDLISRTHFARFLVETGVCKETNEVFRKF
                     LTEGKPGFVPHRWATLKDAVTWITDAGGMAVIAHPARYKFTPNEEFALFTEFKAHGGQ
                     GVEVVTGSHTAAEYVIYADTANEFGLAASRGSDFHSPAESHTELGTLPFLPGNVTPVW
                     EALA"
     gene            complement(869..1507)
                     /locus_tag="PKX82_00435"
     CDS             complement(869..1507)
                     /locus_tag="PKX82_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOF50737.1"
                     /translation="MAIEIVYQDDAIVVLNKPSGLLSVPGRGADKQDCLSSRVQAVFP
                     DALVVHRLDMATSGLLVFSRSLAHQRTLGNAFAAREVHKRYVAIVSGLLTTPPDDWHT
                     IDLPIIVDWPNRPLRIIDRTLGKPSTTRYRSLLANPQDNTTRVELEPVTGRSHQLRVH
                     LQALGHPILGDTLYAPGRVALQAPRLLLHATHLRFNHPSHGTPIEFDCPAGF"
     gene            complement(1497..3641)
                     /locus_tag="PKX82_00440"
     CDS             complement(1497..3641)
                     /locus_tag="PKX82_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF50738.1"
                     /translation="MKRGATQDVIIDGYAPAQMIEDLRKYQSELEIQNQALRYSQQEA
                     EGASERFATLFSNVPLALMVVDEDGLVLASNAMALRLFQPLESDPSLNFLLPFVGATH
                     ADEVAVAFINARTAGTSEVNEVEFLAGAQGRFTGDLHIARIDNPLDNLAHFICAVIDQ
                     GPLLAERKALQKSADVLRQRNEDLLLSEKRMAAIINSSLDAILCIDQNQCITVFNPAA
                     TALFECPVEQAYGSPLARFLPDVQDALTDGKVFSQATLGEFQAVTLLGRQIFVEISVS
                     IERRLRARSPLLPTAGHEASLRNPERSSGAVTTIFARDLTTRKKAEAQRIALETQLRE
                     SQKMQAMGTMAGGIAHDFNNILSAILGNVELAKQDTSVESSAATSLDEIEKAGRRARD
                     LVRQILTFSRNEQPKRIPIQLGEVVQETVRLVKVTLPPTVDLKTIMPYTSPPVLADAT
                     QVEQALLNVCTNAILAMGTDKGSVTIELGQTWLDAPLCGRLGLPPGAYVSLTVRDTGN
                     GMSQATMERIFEPFFTTRQVGQGTGLGLAVVHGIMQTHHGAIDVQSTLGTGSVFTLYF
                     PVSEQAAFATQVDLAPVTDVQGQGKHVMYVDDDQALVFLVKRALTRKGFLVTTFTDPI
                     EAQAALAADPGRFDLLVTDYNMPGYSGLDLLRAVKTICPDLPVALASGYVTPEIEKGA
                     VEAGARALIYKPNDISELCETVQRLITESHGD"
     gene            complement(3759..5633)
                     /locus_tag="PKX82_00445"
     CDS             complement(3759..5633)
                     /locus_tag="PKX82_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOF50739.1"
                     /translation="MCLAIALHSVTYARPVEDTMAQRTLACTACHGEQGRAGPDGYYP
                     RLAGKPAGYLYNQLVNFREGRRHYTLMRGLIDPLDDSYLREVAQHFAALDLPYSPPAK
                     PDTPALVLEHGRKLVNNGDASRKIPACTQCHGAALTGVVPNVPGLLGLPRDYINGQLG
                     GWRTGQRNAPAPDCMGHVAKSLSNQDISAVSAWLSTQAVPGNGKPALTAPPKPPGAPD
                     IQCGKDTTAETSRTPSDAQVARGSYLARIGNCALCHTARGGEPFAGGRDIKTPFGTVF
                     SSNISPDKHFGIGGWTADDFWQAMHHGRSRDGRLLNPAFPYTSYTHVTREDSDALWAY
                     LRSLKPSTRTNTPHAMQWPYGTQAALWAWRTLYFTPAGAASTRPAIQDSQITRGQYLV
                     QGLGHCTECHAQRNVLGGLAKGAQGLGGVIPGSQWVAPSLYRSEESSVAGWATEDIQA
                     FLRTGLTPRAIASGPMAEVVLHGTQYLTEGDATAMARYLKTLGPALATATAPTAPTTA
                     AQSLPPTGRGTALYDKHCAQCHGNAGEGYTKAYPALAGNRAVNMANTSNLALKVLYGG
                     FGPATAGHPRPYGMPPYRLLLSDAEIADVLTYIRSSWGNAGAAVPEFDINRIRTTSAP
                     "
     gene            complement(5699..7390)
                     /locus_tag="PKX82_00450"
     CDS             complement(5699..7390)
                     /locus_tag="PKX82_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF50740.1"
                     /translation="MTAPITVHPGDLPLQCLYRWERDRPDAIYLTQPIGGGQVTDITW
                     AQAADQVRRMAAWLQAQGWPEGSRIAIIGKNSAHWILADLAIAMAGHISIPIYPTFNG
                     ESLTYILEHSDTKACFVGKMDDTSALRTGVPQGLPLIALPLAPALPAMQWSDLIVKTA
                     PLKGTPSPDADTLFTIIYTSGTTGKPKGVMHSYRTMAWGVSSATRRFTMTSADRYISY
                     LPLAHVAERMLIEQASLRYGGRIFFAEALDTFVQDLQRARPTIFFSVPRLWVKFQQGV
                     HSKMLEEKLKRLLSIPLVGRLVRKKILKGLGLDQCRIAAGGAAPMPHEMLHWYRNLGL
                     SLIEVYGMTENCGVSHSTLPGSNQIGTVGQPYDEVQCRIDPETGEIQMRCPAVMLGYY
                     REPEQTAQTITADGWLHTGDKGSVNNQGLLHITGRVKDIFKTSKGKYIAPAPIEDLLM
                     MHQDVEACAVTGANMTQPLGIVLLSQDAVNRSVTPEGKQALTASLEDHLRTVNTQLEA
                     HEKLAYLAAVTTLWTPENGFVTPTLKVKRPRVEDVYGQHFDTWLKRGKPVVWADA"
     gene            complement(7484..7819)
                     /locus_tag="PKX82_00455"
     CDS             complement(7484..7819)
                     /locus_tag="PKX82_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOF50741.1"
                     /translation="MPIAASQPLQATESQVRIEPAGWVCSAAPLQSILQATQQAGIAL
                     PSACRNGTCRTCLSQLVSGEIGYLIEWPGVSAEERQQGLFLPCVACARSDIVIDQPIA
                     RRSSEFNVT"
     gene            7908..8435
                     /locus_tag="PKX82_00460"
     CDS             7908..8435
                     /locus_tag="PKX82_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF50742.1"
                     /translation="MSSTTTFSVRPATLRDAKNIAELHNATVREAFKALITDTPIPVV
                     PLDKRQAYWREAIEYSEPQVQVALDDDKIVGFVGFDRCRDKGTPPTMGEIWAIYVNPA
                     YWAKGVGLALWDAAREGLQEEGCTHVCVWVPIANERALRFHELAGFKREMSSAKTVPM
                     GAVKVEEIRLKRALA"
     gene            complement(8441..9559)
                     /locus_tag="PKX82_00465"
     CDS             complement(8441..9559)
                     /locus_tag="PKX82_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOF50743.1"
                     /translation="MHRVAISSTGLFTPPYVITNEELVESFNQYVALENAKHAAEISA
                     GTRTALTESSVEFIEKASGIQRRYVLEKSGILDPQRMRPRFEARPDSELSLMAEIAVK
                     AAQDALTRAGKTAADVDGVICAAANMQRAYPAMGVEIQTALGVQGYAFDMNVACSSAT
                     FALEMAFNAVRTGSARAILVVNPEITSAHHAWMDRDCHFIFGDVCTAILVERLDLAPA
                     GAFEILGTRLLSTFSNNIRNNAGFMSRAEDRNPEDRDQLFRQEGRKVFKEVCPMAAEH
                     MEKHLATLDLSPQNVRRYWLHQANQSMNQLISRKLLGRDATLDDAPVILNEFANTASA
                     GSIIAFHRHHSDIQPGEVGVICSFGAGYSIGSAIVRKL"
     gene            9713..10738
                     /locus_tag="PKX82_00470"
     CDS             9713..10738
                     /locus_tag="PKX82_00470"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HOF50744.1"
                     /translation="MNMVRAWAATQCGGPLEKFEYDLGEMGAEDVEIAVEHCGVCHSD
                     LAMVDSEWFPATYPLVPGHEVVGTIVAVGAQTKGRKVGQRVGLGWHSASCTHCQYCLG
                     GEQNLCRKSQPTIVGRHGGFAERVRAHWTWSVPLPEALDPGAAGPLMCGGGTAFLPFV
                     IHDIKPTDRVGVVGIGGLGHLAVKFARAWGCEVTAFTSTASKREEALLLGAHHTISSV
                     DVKELKSIAGTLDFLLVTVGASLEWDALIATLAPKGRLHMVGVVTEAMKVRTSGLLSW
                     QRSISASPTPSPVTLAKMLEFSARHGIAPQVEHFPMSRVNEALDHLRAGKARYRVVLD
                     ADFSPAG"
     gene            complement(10748..11218)
                     /locus_tag="PKX82_00475"
     CDS             complement(10748..11218)
                     /locus_tag="PKX82_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOF50745.1"
                     /translation="MTTTVTVELGYEFDVNASAKEVFEVLSNVPDSASHYPKVEKLTD
                     LGGGAYRWEMAKIGIGSVNLQTVYAAKYVSNKAKGTVTWTPVPGEGNALVSGSWKITG
                     NKKSTNLVLNITGELTLPVPALMKMVVEPLVEAEFERMTEQYIANLATRFGGEA"
     gene            11374..13494
                     /locus_tag="PKX82_00480"
     CDS             11374..13494
                     /locus_tag="PKX82_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOF50746.1"
                     /translation="MTKALSINSIDAAVDHVLDTIPGDIVLGIPLGVGKPNPFVNALY
                     QRIKANPARKLRIVTALSLERPVGHSDLESHFLAPLVERVFGNYPDLDYVKDSRAKAL
                     PANIEVREFFMKTGDYLGNAAAQLGYISTNYTFVARDMALQGMNVIAQAVAAREVEGK
                     LMLSLSSNPDVTFEVVEKMRAVGQKLMAIGVINREMPYMPNGAEVPPDFFDVVVTDPA
                     ATHAVFAPPNAKVTPADYAIGLHASSLVVDGGTLQIGIGSLGDAIGQALIVRDRHGPE
                     YQRVLESLCPDGIADRSLGRFNEGLYGCSEMFVNAFLRLIDAGIIRREVYADEALQTL
                     CNTGVVRDGAVTPEVLTALLEARRIRAPLRVKDVVFLKRFGIFKSGVEMKGDQLSVEG
                     AHCSNDLRDAASFDVVCRSMLGSHLQGGVYMTGGFFLGPRDFYEHLRSMAPHDLSRID
                     MTRIDFINQLYGDDSLKRAQRVKARFINTTMKVTLLGAAASDALESGQVVSGVGGQYN
                     FVAMAHALPDARLLMMLRATHDNKDGLKSSIVWNYGHVTIPRHLRDIVITEYGVADLR
                     GQSDGEVVKRLIAIADSRFQDDLVREAQAHGKLEADYELPERYRNNLPQALEATLHPW
                     AEAGLLPDFPFGTDLTEDELRMVRAMKKVKHASEHPAELLTMAVKSLWEGKEAPPAYL
                     ERLGLADAHSFKDLLIRRLFAGNL"
     gene            13536..14432
                     /locus_tag="PKX82_00485"
     CDS             13536..14432
                     /locus_tag="PKX82_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HOF50747.1"
                     /translation="MTTSLLALIDDIATVLDDVAVLTKVAAKKTAGVLGDDLALNAQQ
                     VAGVTADREIPVVWAVCKGSLVNKVILVPAALLISIVLPWAVTPLLMVGGLYLCFEGF
                     EKLVHKYLGHAEDDAAHEAELMQALADPAVDLVALERDKIKGAIRTDFILSGEIIAIT
                     LGTVQASPWTTQLTVLSGVALLMTVGVYGIVAGIVKIDDAGLYLSRRPGEGGGVRAVR
                     AFGRGLLRSAPWLMKTLSVVGTAAMFLVGGGILVHGLPFLHHAVEAIAVPMLPDLVVG
                     VVAGALALAGVMLTQRLLRVFK"
     gene            14450..15397
                     /locus_tag="PKX82_00490"
     CDS             14450..15397
                     /locus_tag="PKX82_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF50748.1"
                     /translation="MSAEKIASKSVATGLLLAVLGAIAFSGKAIIVKLAYRHGVDAVT
                     LIMLRMLFALPFFVCIAWWTGRSRGGVKPTPLTGKDWLGVVWLGVTGYYLASYLDFAG
                     LQYISASLERLILYLNPTLVLLLGLVLYGRGISHRQLVGMAISYSGVLLVFGHEVTLT
                     GSDVALGALLVFLSAVSYAVYLSYSGELVKRLGSLRLVGLATSVACVLCIGQFVVLRP
                     LASAWTVAPEVVWLSVLNATACTVVPVLLVMMAIERIGSGLAAQVGMVGPMSTITMGV
                     LLLGEPFTGWVVAGTVLVVAGVFVVSRVTRMPDTVQMDK"
     gene            15414..>15784
                     /locus_tag="PKX82_00495"
     CDS             15414..>15784
                     /locus_tag="PKX82_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF50749.1"
                     /translation="MDLGLQGKWALVCGASKGLGLGCAQALVQEGVNVLIVARGLAAL
                     ERSASKLVADYADKTGAAVQFCAEDITTVDGRDAVFSMRQDFDIVVTNAGGPPPGNFR
                     DWDRDAWIKAVDANMLTPIEL"
BASE COUNT         2784 a         4511 c         5224 g         3265 t
ORIGIN      
        1 gccagggctt cccacacagg ggtcacgttg cctggcagga agggcagggt gccgagttcg
       61 gtgtggcttt ccgccgggct gtggaagtcg ctgccgcgcg aggccgccag accgaattcg
      121 ttggcagtgt cggcgtagat aacgtactcg gcggcggtgt ggctgccggt gaccacttca
      181 acgccttgtc cgccgtgggc cttgaattcg gtgaacagcg caaattcttc gttgggtgtg
      241 aacttgtagc gtgcggggtg ggcaatcacg gccatgcctc cagcgtccgt gatccaggtg
      301 acggcatcct tgagcgtggc ccagcggtgc ggcacaaagc ccggcttgcc ttcggtgagg
      361 aatttgcgga agacctcgtt ggtttccttg cagacacccg tttccaccag aaagcgcgca
      421 aagtgggtgc gcgagatcag gtcggggttg ccgacaaatt gcagcgcacc ttcaaaagcc
      481 cccttgatgc cgaccttggc cagcccatcc gccatttcca tggcgcgttc ccggcggcct
      541 ccgcgcgtct ggcgcagtcc gttggcaagc cgttcgtcgt ccgcatcaaa ccccaggccg
      601 acgatatgta ccgtttgccc gacaaaggtg acggaaactt cgacacccgt gaggtatttc
      661 atgcccagct cccgggccgc agcggccgca cggtactgtc cgccgatctc gtcatggtcg
      721 gtcagcgccc acagttccac gccgttggca tgggcgcgcg cagccagtgc ctcgggcgtc
      781 agggtcccgt cggaaaccac ggagtggcag tgcaggtcgg cgttgagtag ggtggtcaca
      841 cggccatttt aggtgcaggg ccggattctc agaacccggc agggcaatcg aactcgatgg
      901 gcgtgccgtg cgatgggtgg ttgaagcgga gatgtgttgc gtgcagcagc agtctggggg
      961 cctgtaacgc cacccgtccc ggggcgtaca gtgtgtcgcc caggatggga tggcccaggg
     1021 cctgcaggtg cacgcgcaac tggtgcgagc ggcccgtgac cggctccagt tcgacgcggg
     1081 tggtgttgtc ttgggggttg gctaggaggc tgcggtaccg ggtggtgctg ggttttccaa
     1141 gcgtgcggtc gatgatgcgc agggggcggt tgggccagtc cacgatgatg ggcaggtcga
     1201 tggtgtgcca gtcatcgggc ggcgtggtca gcaggccact gacgatggcg acatagcgct
     1261 tgtgtacctc gcgcgcagcg aaagcgttgc ccagcgtgcg ctggtgcgca aggctccgcg
     1321 aaaacaccag cagacccgag gtggccatgt cgagccggtg cacgaccagc gcatcgggga
     1381 agacggcctg cacgcggctg ctcaggcaat cctgtttgtc cgcgcccctg ccaggaaccg
     1441 acagcaagcc ggagggtttg ttgagtacga cgatggcgtc atcctggtag acgatctcaa
     1501 tcgccatggc tctccgtgat caggcgttgc actgtttcgc acagctcgct gatgtcattg
     1561 ggtttgtaaa tcaatgcgcg ggctccggcc tctacggcac ctttttcaat ttctggtgtg
     1621 acatagcccg aggccagtgc gacgggcagg tccgggcaga tggtcttgac ggcgcgcagc
     1681 agatccagcc cgctgtagcc gggcatgttg tagtcggtca ccagcaggtc aaaccggcca
     1741 ggatcggcgg ccagtgcggc ctgggcctca atcggatcgg tgaacgtggt gacgagaaaa
     1801 cctttgcgcg tgagtgcgcg cttgacgaga aagaccagtg cctggtcatc atccacgtac
     1861 atgacatgtt tgccctgacc ctggacatcc gtgaccggtg cgaggtcgac ctgcgtggcg
     1921 aaagcggcct gctcggaaac cgggaagtac agggtgaaca cactgcccgt tcccagtgtg
     1981 ctttgcacat cgatggcacc gtggtgcgtc tgcatgatgc cgtgtaccac cgccagcccc
     2041 aatcccgtgc cctggcccac ctggcgtgtc gtgaagaagg gctcgaagat acgttccatg
     2101 gtggcctgcg acatgccatt gcccgtgtcg cgcaccgtga ggctgacgta ggcgccgggt
     2161 ggcaggccga gccggccgca cagcggggcg tccagccacg tctggcccag ttcgatggtg
     2221 accgagcctt tgtccgttcc catggccaga atggcattgg tgcacacgtt gagcaaggcc
     2281 tgctccacct gcgttgcgtc ggccagtacc ggtggactgg tgtaaggcat gatcgtcttg
     2341 aggtccacgg tgggtggtag cgtgaccttg accaggcgga ccgtctcctg caccacctca
     2401 cccagctgga tgggaatgcg cttgggctgc tcattgcggc tgaaggtcag gatctggcgc
     2461 accagatctc tggcgcgccg tccggccttc tcgatttcat cgaggctggt ggccgcggac
     2521 gattcgacgg aggtgtcctg ttttgcgagt tcgacattgc ccagaatggc gctcaggatg
     2581 ttgttgaagt catgggcgat gccgcccgcc atggtgccca tggcctgcat tttttgcgat
     2641 tcgcgcagct gcgtttccag tgcgatgcgc tgggcctcgg ctttcttgcg ggtggtgaga
     2701 tcgcgcgcaa agatggtggt gaccgcaccg ctggatcttt cagggttgcg cagactggcc
     2761 tcgtgtcccg cagtgggcaa caacggggat cgtgcgcgca gtcgccgttc gatcgacacg
     2821 ctgatttcca caaaaatctg gcgccccagc aaggtgactg cctggaattc tcccagtgtg
     2881 gcttgcgaga acactttgcc atcggtcagc gcatcctgca catcgggaag gaagcgggcc
     2941 agcgggctgc cataggcttg ctcaaccggg cactcaaaga gtgccgtcgc agcgggattg
     3001 aaaacggtga tgcactggtt ctggtcgatg cacagaatgg cgtccagcga ggagttgatg
     3061 atggctgcca tccgtttctc gctgagcagc aggtcctcat tgcgctggcg cagcacatcg
     3121 gcgcttttct gcagcgcctt gcgctccgcg agcaacgggc cctggtcaat gacggcacaa
     3181 atgaagtgcg ccaggttgtc cagtggattg tcgatgcggg cgatgtgcag gtcgccggta
     3241 aacctgccct gggcgcctgc caggaactcc acttcattga cttcgctggt gcctgcggtg
     3301 cgcgcattga taaacgccac cgccacttca tcggcgtggg tagccccgac aaagggcaac
     3361 agaaagttga gcgacgggtc actttccaat ggctggaaca gacgcaacgc catcgcattg
     3421 ctggcgagca ccagcccgtc ctcgtccacc accatcagag cgagcggcac attcgaaaac
     3481 agggtggcga aacgttcaga cgctccttcg gcttcctgct ggctgtaccg cagggcctgg
     3541 ttctggatct ccagttcgga ctggtacttg cgcaggtcct cgatcatctg cgcgggggca
     3601 tacccgtcga tgattacgtc ctgtgtggca ccacgcttca tgacgttggc tgcgatcggg
     3661 cgcagcctgg cctcttgcgc aatcagtttt tttctacggg aaagtcggac catgttattg
     3721 cctggtggcg aacgagtttg ggcttctcat ggcaggtctc agggagcgga tgtggtgcga
     3781 attctgttta tatcaaactc gggcactgcc gcgcccgcat tgccccagga agaacggatg
     3841 taggtcagca cgtcggcgat ctctgcatcg gagagcagga gccggtaggg tggcatgcca
     3901 tagggcctgg ggtggcccgc cgtggcgggg ccaaatcccc catacagcac cttgagtgca
     3961 aggttgctgg tgttcgccat gttgacggcc cgattgcctg ccaaagcggg atacgctttg
     4021 gtgtaacctt cgccggcatt accgtggcat tgcgcacagt gtttgtcata aagagcagtc
     4081 ccacgcccgg tcgggggcag ggattgggcg gctgtcgtcg gcgcggtagg tgctgttgcc
     4141 gtggcgagtg cgggccccag cgttttcaga tagcgtgcca ttgccgttgc gtcaccttcc
     4201 gtgaggtatt gggttccgtg cagtaccacc tcggccatgg gtccactggc gatggcacgc
     4261 ggcgtaagac ccgtacgcag aaacgcctgg atatcttcgg tagcccagcc ggctaccgaa
     4321 gattcttcac tgcggtagag cgagggcgca acccactggc tgcctgggat caccccaccc
     4381 aatccctgtg cacccttggc gagtccgccc aggacattgc gctgggcatg gcattccgtg
     4441 cagtgcccca gaccctgcac cagatattgg ccgcgtgtga tctgactgtc ctggatggct
     4501 gggcgggtgg atgcagcccc tgccggggtg aagtacaagg tgcgccaggc ccacagggcg
     4561 gcctgcgtgc cgtaaggcca ctgcatcgcg tggggggtgt tggtgcgggt ggagggtttg
     4621 aggctgcgca ggtaggccca cagggcgtcc gaatcctcgc gtgtcacatg ggtgtagctg
     4681 gtgtagggaa aggccgggtt caacaggcga ccgtctcggg atcttccgtg gtgcatggcc
     4741 tgccagaagt cgtcggccgt ccagcccccg atcccaaagt gcttgtctgg cgagatgttg
     4801 ctggagaaaa cggtgccgaa cggtgttttg atgtcccgac cgccggcaaa tggctcgccg
     4861 ccacgggcgg tgtggcacag cgcacagttg ccaatgcggg ccaggtagct gccacgggct
     4921 acctgtgcat cacttggggt gcggctggtt tcggccgtcg tgtcttttcc gcactggata
     4981 tcgggcgcac caggcggttt gggtggggca gtcagcgcag gcttgccatt gcccggcacg
     5041 gcctgggttg aaagccaggc gctgaccgca ctgatgtcct gattgctcag actcttggcg
     5101 acatggccca tgcagtccgg ggcaggggca ttgcgctggc cggttctcca tcctccgagt
     5161 tggccattga tgtagtcgcg gggcaacccc agcagccccg gaacgttggg gacgacgccg
     5221 gtcagggctg cgccatggca ttgggtgcag gccgggatct tgcgggatgc gtccccgttg
     5281 ttgaccagct tacgcccgtg ctccagaacc aatgccggtg tgtccggctt cgctgggggg
     5341 gaatacggca ggtccagtgc ggcgaagtgc tgggcgactt cgcgcaggta actgtcatcg
     5401 agtgggtcga tcaagccgcg catcagggtg taatggcggc gcccttcccg gaaattgacc
     5461 agctggttgt acaggtagcc cgccggcttg ccagcgagtc gcgggtagta gccgtccggg
     5521 ccggcgcgac cctgctcacc gtggcatgca gtgcacgcca gcgtgcgctg cgccatggtg
     5581 tcctcgacgg gacgggcata cgtaacggag tgaagtgcga tggccagaca catcgcgaga
     5641 gccctgcggc catgttgaaa gtacatggca tgagcttacc gcagggggat tcaggttatc
     5701 aggcgtcggc ccagacgacg ggcttgcccc gcttcagcca agtgtcgaaa tgttgcccat
     5761 agacatcttc cacgcgtggg cgtttgacct tgagtgtggg ggtcacaaag ccgttttccg
     5821 gggtccacag cgtggtgact gcagccaggt aagccagctt ctcatgcgct tcgagttggg
     5881 tgttgaccgt gcgcagatga tcctccagcg aggcagtcaa ggcttgcttg ccttccggcg
     5941 tgacactgcg attgacggca tcctgggaca gcaggacgat acccaggggt tgcgtcatgt
     6001 tggcgccagt gacggcacag gcttctacgt cctggtgcat catcagcagg tcttcaatcg
     6061 gtgctggtgc aatgtatttg cccttgctcg tcttgaaaat atccttcacg cgaccggtga
     6121 tgtgcagtaa cccctggttg ttgacggacc ctttgtcgcc ggtatgcagc cagccatcgg
     6181 cagtaatggt ttgcgcagtt tgctcgggct cgcggtagta gcccagcatc actgccgggc
     6241 agcgcatctg gatttctccg gtttcggggt cgatgcggca ctgaacttca tcgtagggtt
     6301 ggcctaccgt cccgatctgg ttgctccccg gcaatgtgga gtgggaaacg ccacagtttt
     6361 cggtcatgcc gtaaacctca atcaggctca gacccaggtt gcggtaccag tgcagcattt
     6421 catggggcat gggtgcagct ccgccggctg caatgcggca ttggtccaag cccagtcctt
     6481 tgagaatttt cttgcgtacc agacgaccga ccagggggat ggacaacagg cgtttgagct
     6541 tttcctcaag catcttggag tgcacgccct gctggaattt gacccacaac cgcggaactg
     6601 aaaagaagat ggtgggacgt gcacgctgca aatcctgcac aaaggtgtcc agtgcctcgg
     6661 caaagaagat gcgtccgcca tagcgcagcg aagcttgttc aatcagcatg cgctcggcca
     6721 catgggccag gggcaggtag gagatgtagc ggtctgcgct ggtcatggtg aaccgtcgcg
     6781 tcgcgctgct cacgccccag gccatggtgc ggtagctgtg catgaccccc ttgggcttgc
     6841 ccgtcgtacc cgaggtgtag atgatggtga acagcgtgtc ggcgtccggc gacggcgttc
     6901 ctttcaaggg cgcagtcttg acgatgaggt ctgaccactg catggccggc aatgcagggg
     6961 ccagcggcag tgcgatcagt ggcagccctt ggggtactcc ggtgcgcagt gcactggtgt
     7021 cgtccatctt gccgacgaag caggctttgg tatcgctgtg ctccaggata taggtgaggg
     7081 attcaccgtt gaatgtgggg tagatcggaa tggaaatgtg gccggccatg gcgatggcca
     7141 ggtcggccag aatccagtgc gcgctgttct tgccgatgat ggcgatgcgg ctgccttcag
     7201 gccatccttg cgcctgcaac catgcggcca tgcggcgcac ctggtctgca gcctgggccc
     7261 aggtgatgtc ggtgacttgt ccgcctccga tgggttgggt cagataaatg gcatcggggc
     7321 gatcgcgctc ccagcggtaa aggcattgca gtggcaggtc gccagggtga acggtaatcg
     7381 gtgcagtcat gaaacaaagg ctcgctaatg aatgccgccg attgttaaag ccattgtttt
     7441 cttcaacaat ggtagtgacc ctaggtgacg ttaaccttcg accctaggtg acgttgaact
     7501 ctgaactccg gcgtgcgatc ggctggtcaa tcacgatatc gctgcgtgcg caggctacac
     7561 acggaaggaa gagtccttgc tgtcgttctt ccgcactcac acccggccat tcgatgaggt
     7621 aaccgatctc acccgagacc aactgactca ggcaggtacg gcacgtgcca ttacggcaag
     7681 cgctgggcaa tgcgatgccc gcctgttgag tggcctgcag aatactttgc aacggtgcgg
     7741 ctgagcacac ccagcctgca ggctcgatgc gtacctgact ctcggtggct tgcaggggct
     7801 gactggcggc aatgggcatg ggttgttttt gcaggggagg cgagtcattc gcatttgcag
     7861 cgacaattgc actctttttc aattttgctt caggtttacg tttagacatg tccagtacga
     7921 ccactttctc ggtgcgcccc gcgactttgc gcgatgccaa aaatattgca gaactccaca
     7981 acgcgaccgt ccgcgaagcc ttcaaggcct tgatcaccga tacgcccatt cccgtcgtgc
     8041 ccttggacaa gcgccaggcc tattggcgtg aggccatcga atacagcgaa ccccaggtgc
     8101 aggtcgcgct ggatgacgac aagatcgtag gctttgtcgg tttcgaccgg tgccgcgaca
     8161 agggcacgcc tccgaccatg ggcgagatct gggctattta cgtcaacccg gcctattggg
     8221 ccaagggcgt ggggctggcg ctatgggatg ccgcccgcga aggcttgcag gaagagggct
     8281 gcacccacgt gtgtgtgtgg gtgccgattg ccaatgagcg tgcactgcgt tttcacgagc
     8341 tggccggttt caagcgcgaa atgtcgagcg ccaagacggt gcccatgggc gccgtcaagg
     8401 tcgaggaaat tcgcctgaaa cgggccctgg cctaagccac tcagagttta cggacgatgg
     8461 cgctgccgat ggagtagccg gcgccgaagg agcagatcac ccccacttct ccgggctgga
     8521 tatcggagtg gtggcggtgg aacgcgatga tggagcctgc cgacgccgtg ttggcaaact
     8581 cgttgagaat cacgggggca tcgtccagcg tggcatcgcg ccccagcaac ttgcggctga
     8641 tgagctggtt catcgactgg ttggcctggt gcagccaata ccggcgcaca ttctggggcg
     8701 acaggtccag cgtggccaga tgcttctcca tgtgttcggc cgccatggga cacacttcct
     8761 tgaacacctt gcggccttcc tgacggaaca gctgatcccg gtcttcgggg ttgcggtctt
     8821 ccgcacggga catgaagcct gcgttgttgc ggatgttgtt gctgaaggtg ctcagcagcc
     8881 gtgtgcccag tatctcgaag gcgcccgcag gtgccaggtc cagtcgctcc accaggatgg
     8941 cggtgcagac gtcgccaaaa atgaaatggc agtcgcggtc catccaggca tggtgggccg
     9001 acgtaatctc ggggttcacg accagaatgg cacgcgcgga gccggtgcgc accgcgttga
     9061 acgccatttc cagggcaaac gtggccgatg aacaggccac gttcatgtca aacgcgtagc
     9121 cctgcacgcc cagtgcggtc tgtatttcaa cgcccatggc cgggtaggcc cgctgcatgt
     9181 ttgccgctgc gcagatgacg ccatccacat ccgcggccgt ttttccggcg cgcgtcagcg
     9241 cgtcctgtgc tgccttgacg gcgatctcag ccatcagcga caactcgctg tcggggcgag
     9301 cttcaaagcg gggacgcatg cgctgggggt ccagaattcc ggacttctcc agcacatagc
     9361 ggcgctggat acccgacgct ttttcgatga attcgacgct ggactcggtc agtgccgtgc
     9421 gcgtaccagc agagatctcg gcggcgtgtt tcgcgttttc cagggcaaca tattggttaa
     9481 aggactcaac caactcttcg ttggtgatga cgtagggcgg ggtgaacagc ccggtgctgc
     9541 tgatggcaac gcgatgcata gtggtacttg aaaaaatagg ggcgctggcc tagtccagcg
     9601 ccgcgctgat tatgctccgg gtttttgcag tcacgcgcgt tacaccgtgg caagccatgc
     9661 cattcaattg gtattacgct tcggagcagt gacagaccaa cagaaagttt ccatgaacat
     9721 ggttcgcgcc tgggccgcca cacagtgtgg tgggccacta gaaaaatttg aatacgacct
     9781 gggggagatg ggcgccgaag acgtcgagat tgcggtggag cactgtggtg tgtgccactc
     9841 ggacctggcc atggtggaca gcgagtggtt cccggcgacc tatccgctgg ttccgggcca
     9901 tgaagtggtc ggcaccattg tggcggtcgg cgcccagacc aagggccgta aggtcgggca
     9961 gcgggtaggt ctgggctggc acagcgcaag ctgcacgcac tgccagtatt gccttggcgg
    10021 cgaacagaat ctgtgccgca agagccagcc caccattgtc gggcgccatg gtgggtttgc
    10081 cgagcgtgtg cgcgcccact ggacctggag cgtgccgctg ccagaggcac tggaccccgg
    10141 agctgccggc cccctgatgt gtggtggcgg gaccgctttt cttccctttg tgattcacga
    10201 catcaagcct accgaccgtg tgggtgtggt cggcattggg gggctgggtc acctggccgt
    10261 gaagttcgcc cgtgcctggg gatgcgaagt gacggccttt acctccaccg cgtccaagcg
    10321 ggaggaagcc ttgctgctgg gcgcacacca cacgatttcc agcgtggatg tgaaggagct
    10381 caagtcgatc gccggaacgc tggattttct gctggtgacg gtaggcgcca gtctggagtg
    10441 ggatgcgttg atcgccaccc tggcgcccaa aggccggctg cacatggtgg gggtggtgac
    10501 tgaggccatg aaggtgcgca ccagtggact gctgtcctgg cagcgcagca tctcagcttc
    10561 accaacaccg tcacctgtga cgctggccaa gatgctggag ttttctgcgc gccatggaat
    10621 tgcgccgcaa gtcgaacatt tcccgatgag ccgtgtcaat gaggcactgg accatctgcg
    10681 cgcaggaaag gcccgctacc gggtggtgct cgacgccgac ttcagtccgg ctggctagac
    10741 aacaggctca ggcctcgccg ccaaagcgtg tggcgaggtt ggcgatgtac tgctcggtca
    10801 tgcgctcaaa ctccgcttcc accagcggct ccaccaccat cttcatcagg gccggaacgg
    10861 ggagtgtcaa ctccccggtg atgttgagca ccaggttggt ggatttcttg ttgccggtaa
    10921 tcttccagct gccagacacc agtgcgttgc cttcacccgg gaccggtgtc caggtgactg
    10981 ttcctttggc cttgttggag acgtatttgg cggcatagac ggtctgcaga ttcacggagc
    11041 caataccgat cttggccatt tcccagcggt acgcgccgcc gcccagatcg gtcagctttt
    11101 ctaccttggg gtagtggctg gcggaatcgg gcacgttgga gagcacctcg aaaacctctt
    11161 tcgccgaagc attgacgtca aactcgtatc ccagctcgac ggtgactgtg gttgtcatgg
    11221 aatctccctt attgttggtt tgaatcgccg aacatattac tgcagcctga ctggttgcgg
    11281 gtcagcgttc ttcttgaaaa gttgtcctag gtcaagactt tcaggaactg ctggtctgat
    11341 actgattgga gaccatataa gaggagacac accatgacaa aagcactgtc gatcaattcc
    11401 attgacgccg cggtagacca cgttctcgac accatccctg gcgacatcgt gctcgggatt
    11461 cccttgggtg ttggcaagcc caatccgttt gtcaatgcgc tctaccagcg catcaaggcg
    11521 aacccggcac gcaagctgcg catcgtcacc gcactctcgc tggagcggcc tgtggggcac
    11581 agtgatctgg agagccactt cctggcaccg ctggtggagc gggtgttcgg aaattacccg
    11641 gatctggact atgtgaagga ctcccgagcc aaggcgctgc ccgccaacat cgaggtgcgc
    11701 gagttcttca tgaagaccgg ggactacctg ggcaacgcgg ctgcgcagtt gggctacatc
    11761 tccaccaact acacctttgt cgcgcgcgac atggcactgc aaggcatgaa cgtgatcgcc
    11821 caggccgtgg cggcccgtga agtggagggc aagctgatgc tgagcctgtc gagcaacccc
    11881 gacgtcacct tcgaggtggt ggagaagatg cgtgccgtgg gtcagaaatt gatggctatc
    11941 ggcgtgatca accgggagat gccttacatg cccaacgggg cagaagtgcc gcctgatttc
    12001 tttgatgtgg tggtgacaga ccccgccgcc acgcatgcgg tttttgcgcc acccaatgcc
    12061 aaggtaacgc ctgcagacta tgcgatcggc ctgcacgcct ccagtctggt ggtggatggc
    12121 ggaaccctgc agattggtat tggctcgctg ggcgatgcca tcgggcaggc gctgatcgtg
    12181 cgcgaccgcc atggtccgga ataccagcgc gtcctggaat ccctgtgtcc cgacggcatt
    12241 gcagaccgca gcctgggccg tttcaacgaa gggctgtatg gctgctcgga gatgtttgtc
    12301 aacgcctttc tgcgcctgat tgatgccggc atcatccggc gtgaggtgta cgccgatgag
    12361 gcattgcaaa ccttgtgcaa taccggcgtg gtacgcgacg gggcagtcac accggaggtg
    12421 ctcaccgcgc tgctggaggc tcgccgcatc cgtgcgccgt tgcgggtcaa ggacgtggtt
    12481 tttctcaagc gctttgggat tttcaaatcc ggtgtggaga tgaagggcga ccagctgagt
    12541 gtggaaggtg cgcattgcag caatgacctg cgcgatgcgg ccagctttga cgtggtgtgc
    12601 cgcagcatgc tgggttcgca cctgcagggc ggcgtttaca tgactggcgg attctttctg
    12661 ggtccccggg atttttacga gcatctgcgc tctatggcac cgcacgatct gtccaggatc
    12721 gacatgacgc gcatcgattt catcaaccag ctgtatggcg atgacagcct caagcgtgcg
    12781 cagcgggtca aggcccgctt catcaacacc accatgaagg tgaccctgct gggcgcggcg
    12841 gccagcgatg cgctggagtc gggccaggtg gtcagtggcg tgggcgggca gtacaacttt
    12901 gtggccatgg cacatgcgtt gcccgacgca cgcctgctca tgatgctgcg cgccacgcac
    12961 gataacaagg acggactcaa gagcagcatt gtgtggaact acggccacgt caccattccg
    13021 cgccatctgc gcgacattgt gattaccgag tacggggtgg cagacctgcg gggacagtcc
    13081 gacggggaag tggtcaagcg cctcattgcc atcgcggatt cccgcttcca ggacgacctg
    13141 gtacgcgaag cccaggccca cggcaagctg gaagccgact acgagctgcc cgagcgctac
    13201 cgcaacaacc ttccgcaggc gctggaggcc acgctgcatc cctgggcgga agcgggcctg
    13261 ctgccggact ttccattcgg cacagacctg acggaagacg aactgcgcat ggtgcgcgcc
    13321 atgaagaaag tcaagcatgc cagtgagcac cccgctgagc tgctgaccat ggctgtcaag
    13381 agcctctggg aaggcaagga agcgccaccg gcctatctgg agcgcctggg tctggccgac
    13441 gcacacagct tcaaggatct gttgatccgc cgcctgtttg ccgggaatct gtagcgttct
    13501 gctggggccg tcggaaggcc tgttaaattc aggccatgac taccagcctc cttgccctga
    13561 ttgatgacat cgccaccgtc ctggacgacg tggccgtact gaccaaagtc gccgccaaaa
    13621 agaccgccgg agtgctgggt gacgatctgg cactcaatgc gcagcaggtg gccggcgtga
    13681 ctgcggaccg ggagattcct gtggtctggg cggtgtgcaa gggctccctg gtgaacaagg
    13741 tcattctggt gccagccgca ctgctgatca gcatcgtgct gccctgggcc gtgacgccgt
    13801 tgctgatggt gggcggtttg tacctgtgct tcgaaggttt cgagaagctg gtgcacaagt
    13861 acctcggcca tgccgaagac gatgccgcac acgaggcgga actgatgcag gctctggccg
    13921 atccggcggt ggacctggtg gcgctggagc gggacaagat caagggggcc atccgaaccg
    13981 acttcatcct ttccggcgaa atcattgcca tcacgctggg tacggtgcag gccagcccgt
    14041 ggaccacgca actgaccgtg ctctcggggg tggcgctgtt gatgacggta ggggtctatg
    14101 gaattgtggc cggcattgtg aagattgatg acgccgggct gtacctcagc cgccgccccg
    14161 gcgagggtgg tggcgtgcgg gccgtgcgcg cctttggacg ggggctgctg cgttctgcgc
    14221 cctggctgat gaagacgctg tcggtggtgg gtacggcggc catgtttctg gtgggcggtg
    14281 gcattctggt ccatggcctg ccgtttctgc accatgccgt ggaagccatc gcggtgccca
    14341 tgttgcctga tctggtggtt ggcgtggtgg ccggtgcact ggcactggcc ggtgtgatgc
    14401 tgacacagcg tctgctgcgc gtattcaagt aaattggctt ccagcccctg tgtctgctga
    14461 aaagattgct tccaaatcag tagcaacggg tttgctgctg gctgtgctgg gtgcgattgc
    14521 cttcagtggc aaagccatta tcgtcaagct ggcgtaccgg catggcgtgg atgccgtcac
    14581 gctgatcatg ctgcgcatgc tgtttgcgct gccattcttt gtgtgcatag cctggtggac
    14641 cggccggtca cgcgggggtg tgaagcccac gcctctgacc ggcaaagact ggctgggcgt
    14701 ggtctggctc ggggtgaccg gctactacct ggccagctac ctcgattttg ctggtctgca
    14761 gtacatcagt gcctcgctgg agcggctgat cctgtacctc aaccccaccc tggtcctgct
    14821 gctggggctg gtgctgtatg ggcggggcat cagccaccgc caattggtcg gcatggccat
    14881 cagctacagc ggtgtgctgc tggtgtttgg ccacgaggtc acgctgaccg gcagcgatgt
    14941 ggcgctgggt gcactgctgg tgttcctgag tgcggtgagt tacgcggtgt atctgtcgta
    15001 cagcggtgaa ctggtcaaac gactcggctc gctgcggctg gtggggctgg caaccagcgt
    15061 ggcctgtgtg ctgtgcatcg ggcagtttgt ggtgttgcgt ccactggcca gcgcgtggac
    15121 cgtggcgccg gaagtggtct ggctttcggt gctcaacgcc acggcctgca cggtggtgcc
    15181 ggtgctgctg gtgatgatgg ccattgagcg tattggctcg gggttggcgg cccaggtcgg
    15241 catggtgggc cccatgtcca ccatcacgat gggcgtgctg cttttgggtg agccgtttac
    15301 cggatgggtg gtggcgggga cagtgctggt ggtggccggt gtttttgtgg tgagccgcgt
    15361 cacgcgcatg cccgatactg tgcagatgga caagtaacga atggagaatc gccatggatt
    15421 tgggattgca gggtaagtgg gcgctggtat gcggcgccag taaggggctg gggctgggat
    15481 gcgcgcaggc actggtgcag gagggcgtca acgtgctcat cgtcgcacgc gggctggcag
    15541 cactggagcg atctgcttct aaattggtag cagactatgc agacaagacg ggggctgcag
    15601 tccagttttg tgcagaagac atcaccaccg tcgatggccg tgacgcggtg ttttccatgc
    15661 gccaggactt tgacatcgtg gtcaccaatg ccggtggccc gccgcctggc aacttccggg
    15721 actgggaccg cgatgcctgg atcaaggcgg tggacgccaa catgctcacc cccatcgaac
    15781 tgat
//
LOCUS       DAOSJM010000006        14917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4138_length_14917_cov_4.773314, whole genome shotgun sequence.
ACCESSION   DAOSJM010000006 DAOSJM010000000
VERSION     DAOSJM010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14917
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4138_length_14917_cov_4.773314"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PKX82_00500"
     CDS             complement(<1..340)
                     /locus_tag="PKX82_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOF50750.1"
                     /translation="MGNKLYVGNLPYSFRDEDLQQAFAAHGSVSSAKVMMERDTGRSK
                     GFGFVEMGSDAEAQAAINAMNGQQFGGRGLVVNEARPMEPRPPRSGGFGGGGGGGGGY
                     GGGGGRSGGGG"
     gene            complement(558..1760)
                     /locus_tag="PKX82_00505"
     CDS             complement(558..1760)
                     /locus_tag="PKX82_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOF50751.1"
                     /translation="MEALKQGADALFILLGAIMVLAMHAGFAFLELGTVRRKNQVNAL
                     VKILVDFSVSAVVYFLVGYAVAYGTTFFVGAEQLAARNGYELVKFFFLLTFAAAIPAI
                     ISGGIAERAKFWPQLIATAVIVGFVYPFFEGIAWNQHFGFQAWIKAATGEEFHDFAGS
                     VVVHAVGGWIALGAVLLLGARSNRYRKDGAMSAHPPSSIPFLALGAWILIVGWFGFNV
                     MSAQVLDKMSGLVAVNSLMAMVGGTLAALIMGRNDPGFVHNGPLAGLVAVCAGSDLMH
                     PLGALVVGGVAGAIFVVMFTLTQNKWKIDDVLGVWPLHGLCGTWGGLAAGIFGSKALG
                     GLGGVSFLAQLAGTAMGVAWALAGGFLVYGALKATLGLRLSQEEEYDGADLTIHKIMA
                     SPDRESNW"
     gene            1942..3294
                     /locus_tag="PKX82_00510"
     CDS             1942..3294
                     /locus_tag="PKX82_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOF50752.1"
                     /translation="MTAPSPFSVDLLAREPFVRFAVTDIDGVCRGKHLSGEKVAGMLE
                     GGGTIASALFGWDITDELYDNVGFTGFHTGYPDVGLRLDPSTARQLPWDDSRWLILGE
                     HTATDGSPLPICPRQVLKRVLTQAQAMGYTAQVGMEFEWFVLKETELSVRQKEYRHLE
                     TATHGLTNYSPFRMDGQHGFVKDLFDYLPAIGVPLEALHTEAGPGNFEAAIRYGEALQ
                     AADRAVLFKQAVREIGRRHGLLSTFMAKYSTAYAGCGGHLHQSLWQDGVNTFHDASAP
                     HGMSDTLCHFVAGQLHCLPQFMALYAPFINSYKRLVDNNLAPVKPTWAVDNRNASLRV
                     IPGSAQSMRLETRTPGADANPYLAIAAALASGLYGIEKKMELSQPPVTGANVGAEGVE
                     RLPHTLAEATDALDRSDLARDLLGDAFVTHFVQTRQWEVRRFNQAVTDWELQRYLELV
                     "
     gene            3306..3734
                     /locus_tag="PKX82_00515"
     CDS             3306..3734
                     /locus_tag="PKX82_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOF50753.1"
                     /translation="MSIFTTSREFPAAPDAVFAALLDPQRLARWWGPAGFRNTFDTCE
                     VKTGGAWRFTMHGPDGADYPNVATFVVVEPPHRLVIQHENAPQFRLTITLSPVGAGTR
                     IVWEQAFEDAAVAAAVRHIVEPANEQNLDRWMAEVATAPA"
     gene            3879..5060
                     /locus_tag="PKX82_00520"
     CDS             3879..5060
                     /locus_tag="PKX82_00520"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOF50754.1"
                     /translation="MSLIATAQNMQFDAPLPLQSGASICQYALSFETYGTLNADKSNA
                     VLICHALNASHHVAGVYAGQDRSEGWWDNMIGPGKAVDTNRFFVIGVNNLGSCFGSTG
                     PMHVNPDSPSGQVYGADFPVVTVEDWVNAQARLLNRLGIQKLAAVMGGSLGGMQALSW
                     TLQYPERVAHAVVVASAPNLTAENIAFNEVARRAIVTDPDFHGGHFYQHGVIPKRGLR
                     IARMIGHITYLSDDVMNEKFGRRLRDAVAQELAETAGNGRTFGDYKYSTQDVEFQIES
                     YLRYQGDKFAEYFDANTYLLITRALDYFDPARKFGGDLSQALAHASCKFLLVSFTTDW
                     RFSPKRSREIVKALLDNKRDVSYAEIDAPHGHDAFLLDDPRYMGVVSSYFDSIAKELQ
                     P"
     gene            5057..5638
                     /gene="metW"
                     /locus_tag="PKX82_00525"
     CDS             5057..5638
                     /gene="metW"
                     /locus_tag="PKX82_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOF50755.1"
                     /translation="MSDATIQHAIAQLVPEGARVLDLGCGDGAMLDHLQRTRGCTGYG
                     VEIDDANVLACVKRGVNVIQLNLDEGLAMFGDASFDVVLQIDTLQHLRNAEVMLRETA
                     RVGKVGIVAFPNFAHWPNRLSILRGRMPVTRRLPYQWYDTPNIRVGTHADLAELAQRT
                     GLRVLDSFGLQEGRTVRFAPNLRAGTSVYKLAR"
     gene            5750..6640
                     /locus_tag="PKX82_00530"
     CDS             5750..6640
                     /locus_tag="PKX82_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HOF50756.1"
                     /translation="MDTQKPSVFSISPLEFNARLGRADAPLVLDVRREARFAESPRLL
                     AGAVRCAPEDVASLIAASKPRDAAVYCVYGHNVSEDAVAALCRAGWNARSLAGGFEGG
                     QDGVDAADAIAQWRACPPISCVKRPDWGVTGERASRWITRERPKVDRIACPWLIRRFI
                     DPRAEFIYVPTAQVFAEAARLQAVAYDIPGAPVSHAGERCSFDALLQGFDLKDAALDL
                     LATIVRAADTDRLDLAPQAAGLLAMSLGLSRLHNKDDQAILDAAMPLYDALYAWCRDR
                     VIAQDEPHNWKPETMTGTQA"
     gene            6637..7986
                     /gene="chrA"
                     /locus_tag="PKX82_00535"
     CDS             6637..7986
                     /gene="chrA"
                     /locus_tag="PKX82_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOF50757.1"
                     /translation="MSAQQSQGVSFGEALKFWLKLGLISFGGPAGQIAIMHRELVEQR
                     RWISEQRFLHALNYCMLLPGPEAQQLATYIGWLMHRTWGGVVAGALFVLPSLLLLVAL
                     SGLYMAFGTVPVVAAILWGIKPAVAAIVLQALHRMASKTLKNPLKAPVLPSLAAISFI
                     AIAVFSVPFPWVVLVAALCGWMGQRWLPGQFAAGGGHGAGKATADHAPAWIDDDTPTP
                     AHARFKPSRLALLVGVGALLWLLPMAALVAWQGWQGTLAQMGWFFTKAALLTFGGAYA
                     VLPYVYQGAVEHFGWLTAAQMMDGLALGETTPGPLIMVVAFVGFVGGWGQPVLGPEAL
                     FWSGALAATVVTWFTFLPSFIFILAGGPLVESTHGKLQFTAPLAAISAAVVGVIASLA
                     LFFIVHLALPTGATGPFDAKALTLAAVAAVALLRYKVGIIPVIAACAVMGWLASLFV"
     gene            7995..8831
                     /locus_tag="PKX82_00540"
     CDS             7995..8831
                     /locus_tag="PKX82_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HOF50758.1"
                     /translation="MPRFAANLSLMYTELPFPDRFAAAAGDGVKGVEYLFPYAWTAQE
                     LAARLADHGLQQVLFNAPPAGATLAEMASAWDRGARGTACLPGREAEFRAGVEQALHY
                     AQALQCPRVHLMAGCPSHDVPVEQAYDTYLDNLAWAASLAGTVAVDVLIEPINTRDMP
                     DYFLTHQAQAHAVVQAVGAANLKVQMDLYHCQIMEGDVATKLRQYLPGGAVGHIQIAG
                     VPERHEPDTGELNYPYLFAVIDAAGYAGWIGCEYRPRGGQAPGATRAGLGWLPKGGLP
                     VA"
     gene            8871..10334
                     /locus_tag="PKX82_00545"
     CDS             8871..10334
                     /locus_tag="PKX82_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOF50759.1"
                     /translation="MNARIPTRALNTTQVLTEVSGAWDASIVPQLQDYIRVPAKSPMF
                     DADWASHGYIDTVVRNTAAWIEAQKVAGLTLEVVRLEGRTPVLFFEIAATQAASSQTV
                     LMYGHLDKQPEFSGWRNDLGPWTPKIEEGKLYGRGGADDGYAAYAAIAAVQALKTQNT
                     PHPRIVGLIETCEESGSYDLLPYIDALRERMGDVGLVVCLDSGAGNYDQLWLTNSLRG
                     MASGVLKVEILTEGVHSGDASGLVPSSFRIMRQVLDRLEDSATGRLLPASFHCEVPAA
                     RLAEARATAAILGEELYRRFPWAHYDCGGSTTFALPTTTDPLEALLNRTWKPTLSVTG
                     ADGFPDLKNAGNVLRPYTAFKLSLRLPPLVDAAVAVQQLKALLEDNAPYQARVTFEGA
                     ASATGWNAPETAPWFAQAVDAASTAHFGAPCGHIGQGGTIPLMNMLSLGFPRAQMMVC
                     GVLGPKSNAHGPNEFLHIPYAKKLTAAVAQVISHFPG"
     gene            10341..11204
                     /locus_tag="PKX82_00550"
     CDS             10341..11204
                     /locus_tag="PKX82_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOF50760.1"
                     /translation="MYTPTSNSSLSTFIVSDGSNLVVQDWPLPANTPRRGMVIIVHGL
                     GEHAGRYNHVAAKLNEWGFSARSYDQCGHGESAGLPGSLPSDNRLLDDLADMVDAARA
                     QMPKGMPLLLLGHSMGGLVVSRFVSLNIRPVDGLIMSSPALDPGLSGFQKFLVSVLPR
                     IAPNLRVGNGLKPQFISHDPAVVIDYKADPLVHDRISARLARFIATAGPATVAHAPHW
                     TVPTLLMYAGDDHLLNADGSRAFAAAAPRHLVTSVCFESLYHEIFNEYDARPVFAALK
                     QWLDQNVQAAG"
     gene            11201..13267
                     /locus_tag="PKX82_00555"
     CDS             11201..13267
                     /locus_tag="PKX82_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF50761.1"
                     /translation="MSWHHRLLGRLRLRWRGGLPLRYTMAAVVVLGVTLPGLVFFAVE
                     QRLAEKSQNALLERSENAFMAISRVSIGEAMWAIDRNALDAALNRILDNPQVVAVRVD
                     DALAGTSSMERRRAGYSLSLAEETDARRLRRLEQRVDRSGETLGSVTVWFDMAYGQNL
                     LLQRRSEMLLLVALQVLASLMVLMPLLVSRVLKPIERLKEQASALLERTPAQAHAPFV
                     WRRNDELGLLGRHLGQVQVQLRELFAQLQSKNEQLQQLALYDHLTGLPNRALFTDLVQ
                     REILLARRTQQQFAVLFIDLDRFKAVNDTMGHAAGDALLVELARRLREVLRDVDVVCR
                     QSGDEFLVLVRNVEHWEWLGEVADRILQVVEGPVGLANGAARISASIGIARFPDDAED
                     FEPLVKCADIAMYQAKSLGRARYSFFHSELNAQLLAHMELEKQLAQAIRGGELVLHYQ
                     PQVDAVSGVLVGLEALVRWKHPERGLLFPGQFIAVAEESGLIAEMGVWTLREACRQQA
                     LWKARGLQVGTMAVNVSALEFRDHRLLDSLQAALQESGVSPGELEVEITESVLMAETD
                     TSLRIIARLHELGVGIAIDDFGTGYSSLSYLKRLRPTQLKIDRSFVRDADTDSDSRAI
                     VTGVISLAKALGLNMVAEGVETEQQRQFLRDAGCHTLQGFLIARPLAVDALEQWMENN
                     IGTTTT"
     gene            13264..13848
                     /locus_tag="PKX82_00560"
     CDS             13264..13848
                     /locus_tag="PKX82_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOF50762.1"
                     /translation="MNSFSQRVRTLLGVALLAGACGVLQAAPTSSTFEPTQVVQGTTL
                     KLNGAGTRYKVVFRLYDLALYTQSKVSTPEELLALPGPKRLNFVALRDLPGTDLGLVF
                     IKGLSANSSPELVRKHTVSSNRLVEIFSGRSKLTAGDTFAMEYVPGKGTTFYIQGQAQ
                     GAPLGDAEYFGMVLKIWVGSSPADFKLKDALLGQ"
     gene            complement(13857..>14917)
                     /locus_tag="PKX82_00565"
     CDS             complement(13857..>14917)
                     /locus_tag="PKX82_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF50763.1"
                     /translation="MATLPVMGYVVGGALSTPLVAKTQSRFGRRGSFQIGLAVAAASA
                     LLGAWAVAGQHFWVLVAATVIAGYYSANGQLYRFAAAELALPAFREKAVSLVLAGGLL
                     GAIAGPNLAARTRNLLEVQFAGAYVVLAAVALVAMVLMAFIRFPDTAVPQGKAHGGRS
                     LWTIVRQPVFAVACITAALGYGVMNLLMAATPLAMQQCGLGFDDAALVLEWHVIGMFA
                     PGFFTGHLIKRFGTLPIMGLGVVLNLLCIAIALSGVELHQFVLALFLLGVGWNFLFTG
                     STTLSMEAYRPEEKDQAQAAINFAVFAVMAFSSLASGALVVTQGWTLLNLGSLPLVLL
                     AGASLLWLGWRQRVTRGG"
BASE COUNT         2791 a         4588 c         4715 g         2823 t
ORIGIN      
        1 agccgccacc accggaacgg ccgccaccgc cgccgtagcc accgccaccg ccaccgccac
       61 cgccgaagcc accggagcgg gggggacggg gttccatggg acgggcttcg ttcaccacga
      121 gaccgcggcc accgaattgc tggccattca tggcgttgat agcagcttga gcttcagcgt
      181 cgctgcccat ttccacgaaa ccgaagcctt tggagcgacc ggtgtcgcgt tccatcatga
      241 ctttggcgct ggaaacggaa ccgtgtgctg caaaagcttg ttgcagatct tcgtcacgga
      301 aagaataagg caggttgcct acgtaaagtt tgttgcccat gtaaggactc ctcaaaataa
      361 ctcaaaacgc gatggagtct aaaaccgcaa tcaacaaacc tatgaagact taaagcagac
      421 gcgaaactga ctaatcaccg caaacttgca caccgatatt tcggcaatgc gaattcatta
      481 tgcgccagtt tgtaaaagca acctaatttt ttttgaaaaa agcaaataat tgttcgaggt
      541 ggtgcagttg tgccaactta ccagttggac tcccgatccg gactggccat gatcttgtgg
      601 atggtcaggt cagctccgtc gtattcttct tcctggctca ggcgcaggcc aagagtggct
      661 ttgagcgcac cgtataccag gaagccgccc gcgagtgccc aggccacacc catggcggtt
      721 ccggcaagtt gggcaagaaa cgacacaccg cccaggccac ccagtgcctt gctgccgaaa
      781 ataccggctg caagcccgcc ccaggtgccg cacaagccgt gcaagggcca gacgcccagc
      841 acatcgtcta tcttccactt gttctgggtc agggtgaaca tcaccacaaa gatggcgccg
      901 gctacaccgc ccaccaccag cgcacccagc ggatgcatca ggtcagaacc cgcgcacacg
      961 gcgaccagcc cggccagcgg gccgttgtgg acaaagcccg gatcgtttct gcccatgatc
     1021 agcgctgcca gcgtaccgcc aaccatggcc atcagtgagt tcactgccac cagaccggac
     1081 atcttgtcca gcacctgggc actcatcacg ttgaagccaa accagccaac aatcaggatc
     1141 caggcgccca gcgccagaaa gggaatgctc gatggcgggt gggccgacat ggcgccgtcc
     1201 ttgcggtagc ggttgctgcg cgcgcccagc agcaggacgg cgcccagcgc aatccagccg
     1261 ccgacggcgt ggaccaccac gctgccggca aaatcatgga actcctcgcc agtggccgcc
     1321 ttgatccatg cctggaatcc gaagtgctgg ttccacgcga tgccttcaaa gaagggatac
     1381 acaaaaccca caatcacggc agtggcgatc agctgcggcc agaatttggc gcgctcggca
     1441 atgccgccgg aaatgatggc gggaatggcc gctgcgaacg tgagcaggaa gaagaacttc
     1501 accagctcat agccgtttcg ggcggccagt tgctcagcgc caacgaagaa ggtcgttccg
     1561 taggcaacgg catatccgac gaggaaatag accacggcag acactgagaa gtccaccaga
     1621 atcttgacca gcgcattgac ctggttcttg cgacggacgg ttccgagttc cagaaacgca
     1681 aatcccgcat gcatggccag caccatgatt gcgcccaaca aaatgaacag ggcatctgcg
     1741 ccctgtttta gtgcttccat gagtaacctc ttgaattaaa tgctttgtat tggtgcaaaa
     1801 aggttctaaa tcccaattgc gcaccaaaac aaagcaaaaa acgagccaat gtgggtatgt
     1861 gccctgtgtc ggttcgccaa gtggtactgc caacgggtgg aaaaactgcg ataaacaggg
     1921 cattccaaaa tgcgagacac catgactgcg ccatctcctt tctccgttga tctgctggcc
     1981 cgagagcctt ttgtccgttt tgccgtgacc gatatcgacg gcgtctgccg tggcaagcac
     2041 ctctccggag aaaaggtggc gggcatgctg gaaggcggcg gcaccattgc cagtgcgctt
     2101 tttggttggg atattaccga tgagctgtat gacaacgtag gcttcaccgg gtttcatacc
     2161 ggttatccgg atgtggggct gcgactggat cccagtacgg cgcgccagct gccctgggat
     2221 gacagccgct ggctgatcct cggtgagcac acggccacgg acggctcgcc gttgccgatc
     2281 tgtccgcgtc aggttctcaa gagggtgctg acccaagctc aggccatggg atacaccgcg
     2341 caggtgggca tggagttcga gtggtttgtg ctcaaggaaa ccgagctttc ggtgcggcaa
     2401 aaggagtacc gccatctgga aactgccacc cacgggctga ccaactattc cccttttcgc
     2461 atggatggtc agcacgggtt tgtgaaggac ctgtttgact acctgccagc catcggcgtg
     2521 ccgctggagg ccctgcacac ggaagcgggt ccgggcaatt ttgaagctgc cattcgctac
     2581 ggcgaagccc tgcaagctgc agatcgggcg gtgctgttca agcaggcggt gcgcgaaatt
     2641 ggtcgccgcc acggcctgct cagcaccttc atggccaagt attccaccgc ttacgcaggg
     2701 tgcggtggcc acttgcacca gagcctgtgg caggatggcg tcaacacctt ccacgacgcc
     2761 agcgcccccc atggcatgag tgacaccctg tgccacttcg ttgccgggca gctgcattgc
     2821 ctgccgcagt tcatggcgtt gtacgcgccg ttcatcaaca gctacaagcg cctggtggac
     2881 aacaacctgg cgccggtcaa gccgacctgg gcggtggaca accgcaatgc ctcgttgcgg
     2941 gtgattcctg gatcggccca atccatgcgt ctggaaaccc gtaccccggg tgccgatgcc
     3001 aacccctacc tggcgattgc ggcggcactg gcctccgggc tgtacggcat cgagaaaaaa
     3061 atggagctca gccagccacc ggtgactggc gcaaatgtgg gtgcggaagg ggtggagcgt
     3121 ctgccgcaca cgctcgctga agccaccgac gcgctggacc gctcggacct ggcgcgtgac
     3181 ctgctcgggg atgcctttgt cacgcacttt gtgcagacac gccagtggga agtacgccgc
     3241 ttcaaccagg ccgtgacgga ctgggagttg cagcgttacc tggagttggt gtaagttcac
     3301 acaccatgtc gatcttcact acctcgcggg aattccctgc cgcaccggac gccgtatttg
     3361 ctgccctgct ggacccgcag cgcctggcca ggtggtgggg acctgcagga tttcgcaata
     3421 cgtttgatac ctgcgaagtg aaaacgggag gcgcctggcg tttcacgatg cacggcccgg
     3481 acggtgcgga ctaccccaac gttgccacgt tcgttgttgt ggagccaccg cacaggctgg
     3541 tcatccagca tgagaacgcg ccgcagttcc ggctgaccat cacactgtcg ccagtcgggg
     3601 caggcacccg gatcgtatgg gagcaggcgt ttgaagatgc cgccgtcgcc gcagcggtgc
     3661 gccatatcgt ggagccggcc aatgaacaga atctggaccg ctggatggca gaggtggcaa
     3721 ccgcgccggc gtagtgcggc cagcattgct acaattcctc cagcgccaat ttgctccagc
     3781 ttgcgggcgc ttaataaatg cagctaaaga cggaccgaca caagtacccg gcctcgtttt
     3841 tgcgacgccg ggttttttat tgggaatacg ggtaagcaat gagtctgatc gccaccgcgc
     3901 aaaacatgca gttcgacgca cctctgccct tgcagagtgg ggcgtcgatc tgccaatacg
     3961 cgctcagttt cgagacctat ggcacgctca atgccgacaa gtccaatgcg gtgctgatct
     4021 gccacgccct gaacgcctcg caccatgtgg cgggcgttta cgcaggacag gacaggagcg
     4081 aaggctggtg ggacaacatg atcggccctg gcaaggcggt ggacaccaac cggttctttg
     4141 tgatcggggt gaacaacctg ggctcctgct tcggctcgac cggcccgatg catgtgaatc
     4201 cggattcacc gtcgggtcag gtgtatggcg ccgacttccc cgtggtgacg gtagaagact
     4261 gggtaaacgc ccaggcccgc ctgctcaacc gactgggtat ccagaaactg gccgccgtga
     4321 tgggcggtag cctcggtggc atgcaggcac tgtcctggac cctgcaatat cccgaacggg
     4381 tcgcgcatgc ggtggtggtg gccagtgcgc ccaacctgac agccgagaac attgcgttca
     4441 acgaagtggc acgccgcgcg atcgtgaccg acccggattt ccacggcgga cacttctacc
     4501 agcacggcgt catccccaag cgcggcctgc gcattgcccg catgattggg cacatcacct
     4561 acctcagcga tgatgtgatg aatgagaaat ttggccgcag gttgcgcgac gccgtggcgc
     4621 aagaacttgc ggagaccgct ggcaatggca gaacctttgg tgactacaag tactccacgc
     4681 aggacgtgga gttccagatc gagagctatc tgcgttacca gggtgacaag ttcgccgagt
     4741 acttcgatgc caacacctac ttgctgatta cccgggcgct ggattacttc gacccggcgc
     4801 gcaagttcgg cggtgatctg agccaggcgc tggcccatgc cagctgcaag ttcctgctgg
     4861 tgagcttcac caccgactgg cgcttcagcc ccaagcgcag ccgcgagatc gtcaaggcgc
     4921 tgctcgacaa caaacgcgat gtgagttacg ccgaaatcga cgcaccgcac ggtcatgatg
     4981 cctttttgct cgatgacccc cgttacatgg gcgtagtcag ctcttatttt gatagcattg
     5041 ccaaggagtt gcagccatga gcgacgcaac catccagcat gccattgccc aactggtgcc
     5101 cgaaggcgca cgcgtgcttg acctgggctg tggcgacggc gccatgctcg accacctgca
     5161 gcgcacccgc ggttgtaccg gttacggcgt ggaaatcgat gatgccaacg tgctggcctg
     5221 cgtcaagcgc ggcgtcaacg tcatccagct caacctggac gaaggactgg ccatgtttgg
     5281 cgacgcctcg tttgatgtgg tactgcagat cgacaccttg cagcacctgc gcaatgcaga
     5341 ggtcatgctg cgcgagaccg cgcgcgtcgg caaggtgggc atcgtagcct tccccaattt
     5401 tgcgcactgg cccaaccgcc tgagcatcct gcggggccgc atgccggtca cccgccgcct
     5461 gccttaccag tggtacgaca cgcccaacat ccgggtggga acgcatgccg acctggccga
     5521 gctggcccag cgcacgggcc tgcgcgtgct cgacagcttt ggtctgcagg aggggcgcac
     5581 cgtgcggttt gcccccaacc tgcgcgccgg aacttcggtg tacaaacttg cgcgctgagc
     5641 cgctgttgtg gtagattgct cccatcgggg tttcagctcc ccggtgtcgg gcctctaagg
     5701 ctccaagatg gtgtcccatc caaatgctgg caacgcaaag gagcattcca tggacaccca
     5761 aaaaccatct gttttttcca tttctcccct ggagttcaat gcccgactgg gccgtgccga
     5821 tgcgcccctg gtgctggatg tgcgtcgcga agcgcgtttt gcggaaagcc cacgcctgtt
     5881 ggccggcgcc gtacgttgtg ctccggaaga tgtggcgtcc ttgatcgctg catcaaagcc
     5941 gcgtgatgcg gctgtgtatt gcgtctatgg ccataacgtc agcgaagacg cggtggccgc
     6001 actgtgccgt gcaggctgga atgcccgtag tcttgccggt ggtttcgagg gcggacaaga
     6061 cggcgtggat gcagctgatg ccattgcgca gtggcgtgca tgccccccga tcagctgtgt
     6121 caaacgcccg gactggggcg tgacgggaga gcgggcctcg cgctggatta cacgggagcg
     6181 ccccaaggtt gaccgcatcg cctgcccctg gctgatccgc cgcttcattg acccgcgcgc
     6241 cgagttcatc tatgtgccca cagcgcaggt gtttgccgag gcggcacgcc tgcaggcggt
     6301 ggcctacgac atccctggtg caccggtatc ccatgcaggc gaacgttgca gttttgatgc
     6361 cttgctgcaa ggcttcgatc tgaaggatgc cgccctcgat ttgctggcca ccatcgtgcg
     6421 cgcagcggat acggaccggc tcgacctggc gccacaggcc gcgggtctgc tggccatgtc
     6481 gctgggtctt tctcgtctgc acaacaaaga cgaccaggcc atcctcgacg ccgccatgcc
     6541 tctgtatgac gcgctgtatg cctggtgtcg tgaccgtgtg atcgcgcagg acgaaccaca
     6601 taactggaaa cccgagacca tgactggaac acaggcatga gcgcgcagca atcccaaggt
     6661 gtcagctttg gcgaagcact gaaattctgg ctgaagttgg gtctgatcag ttttggcgga
     6721 ccggccggac agattgccat catgcaccgc gagctggtgg agcagaggcg ctggatttcc
     6781 gagcagcgtt ttctgcacgc gctgaactac tgcatgctgt tgcctggccc cgaggcccag
     6841 cagctggcca cctacatcgg ctggctgatg caccgcactt ggggcggggt ggtggctggc
     6901 gcgctgtttg tgttgccgtc gctcctgctg ctggtggcgt tgagcgggct gtacatggcc
     6961 tttggtacgg tgccggtggt ggccgccatc ctgtggggca tcaagccggc ggtggccgcc
     7021 atcgtgctgc aggcgctgca ccgcatggcc agtaaaacat tgaaaaatcc gctcaaagcc
     7081 cccgtgctgc cttctctggc agctataagt ttcatagcaa ttgcagtgtt ctccgtgccg
     7141 ttcccctggg tggttctggt ggcagcgctg tgcggctgga tggggcagcg ctggttgcca
     7201 ggccagtttg ccgcgggtgg cggccatggt gctggcaagg ccacggcaga ccacgctccg
     7261 gcgtggattg atgacgacac accaaccccc gcgcatgccc gtttcaaacc gtcccggctc
     7321 gcgttgctgg tgggggtggg cgcgcttctg tggctgctgc ccatggcggc gctggtggcc
     7381 tggcagggct ggcaaggcac gctggcgcag atgggctggt tctttaccaa ggccgctctg
     7441 ctgactttcg gcggtgccta tgccgtgctg ccctatgtgt atcagggcgc ggtggagcac
     7501 tttggttggc tgactgcggc gcagatgatg gacggtctgg cgctcgggga aaccacgcca
     7561 gggccgctca tcatggtggt ggcttttgtg ggttttgtcg ggggctgggg ccagccggtg
     7621 ctcggtcccg aagccctgtt ctggagtggc gcgctggcgg ccaccgtcgt gacctggttc
     7681 accttcctgc cctcgttcat cttcattctc gcaggcgggc cgctggtgga atccacccac
     7741 ggcaagttgc aatttacggc tccgctggcc gccatcagtg cagcggtggt tggggtcatt
     7801 gccagtcttg ctctgttttt tatagtgcat ctcgcactac ccacgggggc tacaggccca
     7861 tttgatgcca aagcgctgac gttggcggcc gtggccgcag tcgccttgct gcgttataaa
     7921 gtgggcatta ttccggtcat tgctgcctgt gctgttatgg gctggctggc cagcctcttt
     7981 gtctgaagat tgccatgccc cgttttgccg ccaacctgag cctgatgtac accgagctgc
     8041 cgtttccgga ccgttttgcg gcggcggccg gcgatggagt caagggtgtg gagtacctgt
     8101 ttccctacgc ctggacagcg caggagctgg cggcgcgtct ggcagaccac ggcctgcagc
     8161 aggtgctgtt caatgcgccg cccgccggtg ccacgctggc ggagatggcc agtgcctggg
     8221 accggggggc gcgcggaacg gcctgcctgc ccgggcgtga agcggagttc cgtgcggggg
     8281 tcgagcaggc gctgcactat gcacaggcac tgcagtgccc gcgcgtgcac ctgatggccg
     8341 ggtgtccatc gcacgatgtc ccggtggagc aggcctacga cacctatctg gacaaccttg
     8401 catgggcggc ctcactggca ggcaccgtcg cggtggacgt gctgatcgaa cccatcaaca
     8461 cccgggacat gccggactac tttctcacgc accaggcgca ggcccatgcc gtggtgcagg
     8521 ccgtcggtgc ggccaacctc aaggtgcaga tggacctgta ccactgccag atcatggaag
     8581 gggatgtggc gacgaagttg cgccagtacc tgcccggcgg cgcggtgggg catatccaga
     8641 tcgccggcgt gcccgagcgc cacgagccgg atacgggtga gttgaactac ccatacctgt
     8701 ttgcggtcat cgatgctgcg ggttacgccg gctggatcgg ttgtgagtac cggccccgtg
     8761 gcgggcaggc tccgggcgcc acccgcgcgg gcctgggttg gctgccaaaa ggtggcctgc
     8821 ccgtggcatg atgtcaggct acggacagct ttcggcagac cagacccacc atgaatgctc
     8881 gcatccccac ccgtgccctg aacaccaccc aagtcctcac cgaggtcagc ggggcatggg
     8941 acgcctccat cgtcccccag ttgcaggact acatacgtgt cccggccaaa tcgccgatgt
     9001 tcgatgcaga ttgggcgtcc cacggctaca ttgatacagt ggtgcgcaac accgctgcgt
     9061 ggatcgaagc gcagaaggtg gccgggctca cactggaagt ggtgcggctg gaggggcgca
     9121 ccccggtgct gttcttcgag atcgccgcaa cccaggccgc ctcaagccag accgtgttga
     9181 tgtacggcca cctggacaag cagcccgagt tctcgggctg gcgcaatgat ctgggcccgt
     9241 ggaccccgaa gatcgaggag ggcaagctgt acggccgcgg tggcgcagat gacggctatg
     9301 cagcgtacgc cgccatcgcc gctgtccagg cgctcaagac ccagaacacc ccacaccccc
     9361 gcatcgtggg cctgatcgaa acctgcgaag aaagcggctc ctacgacctc ctgccgtaca
     9421 tcgacgcatt gcgcgagcgc atgggcgatg tggggctggt ggtgtgcctg gattcgggcg
     9481 cgggcaacta cgaccagctg tggctgacca atagcctgcg cggtatggcc agcggtgtgc
     9541 tcaaggttga aattttgacc gagggcgtgc attcgggtga tgccagcggg ctggtgccat
     9601 cgagttttcg catcatgcgc caggtgcttg accgcctgga agacagcgcc accgggcgcc
     9661 tgttgcctgc cagcttccac tgcgaggtgc cagccgcgcg gctggccgag gcacgcgcga
     9721 cggcggccat tctgggggag gaactctacc ggcgctttcc ctgggcgcac tacgactgtg
     9781 gcggctccac cacgtttgcc ctgcccacca cgacggaccc gctcgaagcc ctgctcaacc
     9841 gcacctggaa acctacactg agcgtgaccg gtgccgatgg atttccggac ctgaagaatg
     9901 caggcaatgt gctgcgcccc tacaccgcct tcaagctgtc cctgcgcctg ccgccgctgg
     9961 tggacgctgc tgtggcggtg cagcagctca aggccctgct ggaggacaac gcaccctacc
    10021 aggcccgggt gacgtttgag ggtgccgcca gcgccacggg ctggaatgcg cccgaaacgg
    10081 cgccctggtt tgcgcaggca gtggatgcag cctctacggc acatttcggc gcaccctgcg
    10141 gccatatcgg acagggtggc accattccgc tcatgaatat gctgtccctg ggtttcccgc
    10201 gcgcacagat gatggtgtgc ggcgtgctgg gccccaagag caatgcccac ggacccaacg
    10261 aattcctcca tatcccctac gccaagaagc tcacggccgc tgtggcgcag gtcatttcac
    10321 atttccccgg ctagcagacc atgtacaccc ctacaagcaa ctcgtccctt tccactttca
    10381 ttgtgagcga tggctccaac ctggttgtgc aggactggcc cctgccggcg aacacgcccc
    10441 ggcgcggcat ggtcatcatc gtgcacggtc tgggcgagca tgccggccgc tacaaccatg
    10501 tggcggccaa gctcaacgag tggggatttt ccgcacgcag ttacgaccag tgtggccatg
    10561 gggaatcggc cggcttgccg ggcagcctgc cctcggacaa ccggttgctg gatgatctgg
    10621 cagacatggt ggacgccgcg cgcgcgcaga tgcccaaggg catgcccctg ctgttgctgg
    10681 gccacagcat gggcgggctg gtggtaagcc ggtttgtgtc gctgaacatc cggccggtgg
    10741 acggactcat catgtcctca ccggcgctgg accccggact cagcgggttc cagaaattcc
    10801 tggtgtcggt gctgccacgc attgcaccga acctgcgggt gggcaatggc ctcaagccgc
    10861 agttcatctc gcatgacccc gcggtggtca ttgattacaa ggccgacccg ctggtgcacg
    10921 accgcatctc ggcacggctg gcacggttca tcgccacggc ggggccggcc acggtggccc
    10981 atgcgccgca ctggacggta cccaccctgc tcatgtatgc gggcgatgac cacctgctca
    11041 acgccgacgg gagccgggca tttgctgcgg ctgcgcccag gcatctggtc acttcggtgt
    11101 gcttcgagtc gctgtaccac gagatattca acgaatacga tgcccgtcct gtgtttgcag
    11161 ccctgaagca gtggctggac cagaacgtcc aggccgctgg atgagttggc atcaccgcct
    11221 gctggggcgg ttgcgcctgc gttggcgcgg cggcctgccg ttgcgctaca ccatggctgc
    11281 tgtggtggtg ctgggggtga ctctgccggg gctggtgttc tttgcggttg aacaacggct
    11341 ggctgaaaaa tcccagaatg ccctgctgga gcgcagtgaa aatgccttca tggcgatcag
    11401 ccgggtatcc atcggcgagg ccatgtgggc gattgaccgc aatgcgctgg atgcagcact
    11461 caaccgcatt ctggacaatc cgcaggtggt cgcggttcgt gttgacgatg cattggccgg
    11521 gacctcttcc atggagcgcc gacgtgccgg ctacagcctg tcgctggccg aggaaaccga
    11581 tgcccgccgc ttgcgccggc tggagcagcg tgtggatcgc agtggtgaga cgcttgggtc
    11641 cgtgaccgtg tggtttgaca tggcctatgg ccaaaacctg ctgttgcaac ggcgttccga
    11701 gatgctgctg ttggttgccc tgcaggtact ggccagcctg atggtgctga tgcccctttt
    11761 ggtcagccgg gtgctcaagc cgatcgaacg cctgaaagaa caggccagcg ccctgctcga
    11821 gcgcacaccg gcccaggcgc atgcgccttt cgtctggcgg cgcaacgatg aactggggtt
    11881 gctcgggcgc catctgggtc aggtgcaggt gcagttgcgg gagttgtttg cccaactgca
    11941 aagcaagaac gaacagttgc agcagctggc gctgtacgac cacctgaccg gtttgcccaa
    12001 ccgggccctg tttaccgatt tggttcagcg cgagatcctg ctggcgcggc gcacccagca
    12061 gcagtttgcc gtgctgttca tcgatctgga ccgtttcaag gcggtcaatg acaccatggg
    12121 ccatgctgcg ggcgatgcat tgctggtgga gctggcgcgc aggttgcgcg aagtcctgcg
    12181 cgatgtggac gtggtgtgcc ggcaaagtgg cgacgaattt ctggtgttgg tgcgcaatgt
    12241 cgagcactgg gaatggctgg gcgaggtggc tgaccggata ctccaggtgg tcgaaggtcc
    12301 ggtcgggctg gctaacggtg ctgcacgtat ttctgccagc atcgggattg cccggtttcc
    12361 ggacgacgcc gaagacttcg agccgctggt caaatgtgcc gacatcgcga tgtaccaggc
    12421 caaatccctg ggccgggccc gttacagctt ttttcacagc gaactcaacg cgcagctgct
    12481 ggcccacatg gagctcgaaa agcaattggc ccaggcgatc cgtggtggcg aactggtgct
    12541 gcactaccag ccccaggtgg acgcggtcag tggtgtgctg gtggggctgg aggcactggt
    12601 gcgctggaag catcccgagc gtggattgct gttcccggga cagttcattg cggtggcgga
    12661 agaatccggg ttgattgccg aaatgggcgt ctggacactg cgggaagcct gccggcagca
    12721 ggccttatgg aaggcgcgcg gcctgcaggt gggcaccatg gcggtcaacg tgtcagcact
    12781 ggagttccgg gatcaccgcc ttctggacag tctgcaggca gctctgcagg aaagtggcgt
    12841 gtcacccggt gagctggaag tggaaatcac cgagagcgtg ctgatggcgg aaaccgacac
    12901 cagcctgcgc atcattgccc ggctgcacga gttgggcgtg ggtatcgcga tcgatgattt
    12961 cggaaccggc tattcctcgc tgtcctacct caagcggctg cgtcctaccc agctcaaaat
    13021 tgaccgctcg tttgtgcgtg acgccgatac cgacagcgat tcgcgggcca tcgtcaccgg
    13081 tgttatcagt ctggcgaaag ctctcggact taatatggtc gccgaaggcg tggagaccga
    13141 gcagcagagg cagttcctgc gcgatgcggg ctgccacact ttgcagggtt tcctgattgc
    13201 gcgaccgctg gcggtcgatg cactggagca gtggatggaa aacaacatag ggacaacgac
    13261 aacatgaatt ctttttcgca acgggtccgc acgctactgg gcgtggcact tctggcgggc
    13321 gcctgtggcg tgctgcaggc agcacccaca agcagcacct tcgagcccac gcaggtggtg
    13381 cagggcacca ccttgaagct caatggcgcc ggaacacgct acaaggtggt tttcaggctg
    13441 tatgacctgg cgctgtacac gcaaagcaaa gtgagtaccc ccgaggagtt gctggcattg
    13501 cctggcccca agcggctgaa cttcgtggct ttgcgcgatt tgcccggtac cgatctgggg
    13561 ctggtgttta tcaagggcct gtctgccaac tcatcgcctg aactggtgcg caagcacacg
    13621 gtttccagca accgcctggt ggaaattttt tcgggccgtt ccaaactgac ggcaggtgac
    13681 acctttgcca tggaatacgt tccgggtaag ggcaccacgt tctacatcca gggccaggcc
    13741 cagggtgctc cgttagggga tgccgagtac ttcggcatgg tgctcaagat ctgggtaggc
    13801 tcctcacccg ccgacttcaa gctcaaggac gctctgctgg gccaataacc agtagctcag
    13861 ccgccccggg tgacccgctg gcgccatccg agccacagca aggaggctcc cgccagcagg
    13921 accaggggca atgaacccag gttcaacagg gtccagccct gggtgacgac cagggcgccc
    13981 gaggccagtg agctgaaggc catcaccgca aacacggcaa aattgatggc cgcctgcgcc
    14041 tggtcctttt cctcgggccg gtaggcttcc atcgacagcg tggtgctgcc ggtgaacagg
    14101 aagttccagc ccacgcccag caggaatagc gccagtacaa actggtgcaa ctccacgccg
    14161 gacagcgcaa tggcgatgca caacaggttg agcaccaccc ccaaccccat gatcggcagg
    14221 gtgccaaagc gtttgatcag gtgcccggta aaaaagcccg gcgcgaacat gccgatcaca
    14281 tgccattcca gcaccagtgc agcatcgtca aagcccaggc cgcactgctg catggccagc
    14341 ggggtggcag ccatcagcag gttcatgacg ccgtagccca atgcggcagt gatgcaggcc
    14401 acggcaaaca ccggctggcg cacgatggtc cacaggctgc gtccgccatg ggctttgcct
    14461 tgcggtaccg ccgtgtccgg gaagcgtatg aaggccatca gcaccatggc gaccagcgca
    14521 acggcggcca gtaccacgta ggccccggca aattgcacct ccagcaaatt gcgggtgcgc
    14581 gctgccaggt tggggcccgc aatggcgccc agcaaaccac cggccagcac cagggaaacg
    14641 gccttttcgc ggaatgcggg cagtgccagt tcggcggctg caaaccggta gagctgtccg
    14701 ttggcgctgt agtaaccggc gatcacggtg gcagccacca gcacccagaa atgctgcccg
    14761 gcgacagccc aggcgcccag cagcgccgac gcggctgcga cggccagtcc gatctggaat
    14821 gagccgcgcc gtccaaagcg cgactgggtc ttggccacca gtggcgtgct cagggcgcca
    14881 ccgacgacat agcccatgac gggtagcgtg gccatcc
//
LOCUS       DAOSJM010000007        14809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4177_length_14809_cov_4.674054, whole genome shotgun sequence.
ACCESSION   DAOSJM010000007 DAOSJM010000000
VERSION     DAOSJM010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14809
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4177_length_14809_cov_4.674054"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="PKX82_00570"
     CDS             complement(<1..1135)
                     /locus_tag="PKX82_00570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOF50764.1"
                     /translation="MKFLFSAFLACLCFAASAQTPQPPEIAARSYLLLDVTANQILAA
                     KDIDAPLEPASLTKLMTAYLVFDALRAKKLDLKQALPVSERAWKMPGSRMFIDPKMQV
                     PVEDLIKGMIVQSGNDATMALAEGVGGTAERFVQMMNEQAKALGMKSTGYRNPEGLTE
                     AGHTTTARDLSILATRLMADFPDYVGFYAIKKYRYPGTPAANDSNRNLLLFRDPTVDG
                     LKTGYTDAAGYCMVATAKREISPAGGSRRLLSIVLGTANENARANESQKLLNWGFVAF
                     EPVKLFDADQAVVTPNVWKGAASTVRLGRNQPIVVVVPAGSAAKLKTQVVRNDPLVAP
                     IAKGQIAGTLRVTSGEQTVVDVPLVALDGVEQAGVVPGEAGQRG"
     gene            complement(1151..1792)
                     /locus_tag="PKX82_00575"
     CDS             complement(1151..1792)
                     /locus_tag="PKX82_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF50765.1"
                     /translation="MNSQTQRMTIDGTAGLMEVLLDEPAPALGVPRGTAVIAHPHPLF
                     GGTMDNKVVQTLARAFVLCGWRAVRFNFRGVGKSQGAYDEGRGELLDYLSVVAHFAPE
                     GSLAVAGFSFGSFVASHAAQALAQTRVVEKLVLVGTAATRFQVAPIAPVLHDRTLVVH
                     GEVDETVPLVDVMDWARPQSLPVTVIPGGEHFFHGQLPLLRNLVVRHLSAPFA"
     gene            complement(1828..2178)
                     /locus_tag="PKX82_00580"
     CDS             complement(1828..2178)
                     /locus_tag="PKX82_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOF50766.1"
                     /translation="MTDSTRTPGSYYERHIFFCLNERKNGEECCAQHQAQEAFDRCKG
                     QVKAAGLAGPGKVRVNKAGCLDRCAAGPVAVVYPEAIWYTYVDLQDIDEIVESHLKNG
                     QVVERLLTPPHLGR"
     gene            complement(2188..3282)
                     /locus_tag="PKX82_00585"
     CDS             complement(2188..3282)
                     /locus_tag="PKX82_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOF50767.1"
                     /translation="MHKTAASPLALMYAVLVVYASLYPFAEWRDQDILPWSFLLAPFP
                     RYWTRFDVAINVAGYLPLGSMLALSALRTGRSRQALWLPVVLAGVLSFAMEALQSYLP
                     LRVPSREDWLLNTLGAALGAAATVLLERMGAIDRWSRFRARWFVPHARGGLVLLATWP
                     LALLFPAAVPFGLGQVFERIEAAIADRLADTPFLDWMPVRDIELQPLVPGAEMVCVLL
                     GLLIPCLLGYCVIRSTWRRLVFAPLCIGVGVLVTGLSAALSWGPVHAWSWLDLPTQLG
                     IAGAIVLALVATLASWRVSAALALLALGVYLSLLNQAPESAYFAQTLQDWEQGRFIRF
                     HGLAQWLGWLWPYAALLYVVVRVGQRNPKN"
     gene            complement(3293..3721)
                     /locus_tag="PKX82_00590"
     CDS             complement(3293..3721)
                     /locus_tag="PKX82_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOF50768.1"
                     /translation="MLWVKSFHIVFVASWFAGLFYLPRIFVNLAMVPADSTAERARLL
                     LMARKLMRFTTLLAVPAVGLGLWLWIGYGIGWGPGNGWMHAKLLLVVLVLGYHHVCGR
                     LLRKFEAGVASRGHVWFRWFNEAPVLMLVAIVVLVVVKPF"
     gene            4080..5102
                     /gene="hemB"
                     /locus_tag="PKX82_00595"
     CDS             4080..5102
                     /gene="hemB"
                     /locus_tag="PKX82_00595"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOF50769.1"
                     /translation="MTYTTPYAPFPAGRPRRLRRDTFTRNLVRENTLTAHDLIYPVFV
                     VEGQGQRVPISSMPGVERLSLDLLLPVAEECVKLEIPVMALFPVIDQSLKNYEGSEAL
                     NPDGLVPRVVRALKKEFPQLGVMTDVALDPFTSHGQDGLPDTRPETEGYILNDETVAM
                     LVQQAMTQADAGVDIVAPSDMMDGRIAAIRTALEAKGHIHTRIMAYSAKYASAFYGPF
                     RDAVGSASNLGKGNKKVYQMDPANTDEALREVAMDIAEGADMVMVKPGMPYLDVVRRV
                     KDEFKVPTFAYQVSGEYAMLKAAAQNGWLDHDAVMMESLLAFKRAGADGVLTYFARDA
                     AKLLRK"
     gene            5170..6270
                     /locus_tag="PKX82_00600"
     CDS             5170..6270
                     /locus_tag="PKX82_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOF50770.1"
                     /translation="MRIFHIQSSTVTESADIDALATSGPPAQGYIWVACAREELESSL
                     GKVQSMLQSLGGAQLVDLHVSDLLNQQLPSHYDYTSQYDMLVFRRLAAGNGSTPGTRI
                     PARGGPPILRRIDTSPVGFVVLDRVLLTVHPTDCAAREAYAAKLLQAASAESRAAGAR
                     LPTSPADLMLRIVNQMVDSYLALRRELTHQLDHWQSELLNPKTRFNNWSTLMDARLAL
                     HHLDEICEEQRSSIQDWIEAVKTWPDATTVADQRERELLQVRSRDVLEHIERVVHHVR
                     RLEQSAETAVQMHFSAQSHRTNDIMRTLTALTAIFLPLNLIAGIFGMNFDFIPLLHIH
                     SGFWWAMGSMALIAMALGLLFWRKRYIARSEH"
     gene            complement(6283..7176)
                     /locus_tag="PKX82_00605"
     CDS             complement(6283..7176)
                     /locus_tag="PKX82_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOF50771.1"
                     /translation="MPNWPLAPVRYAQAAIICIASLASAWAADYTGPLLDAHLHYNEE
                     AWNGQTGPHPLPDVLARMQRNGVRAIVANSRPNDGTKALADAPQTRAAGVTVVPFVRL
                     YRNRADYDNWFRDETIYTLVQTELARGTGAGPYKGLGEFHLYDSANANGPVARKLMQL
                     AEKQQLAVLAHVDDVAIDLLMAHAPKARLIWAHTGIGGAPAARVDALMARYPLLMGEL
                     SYRPGLTCDDGQLCPEWRALVTKYPDRFLIGSDTWVNQRWLYYDELMQGYRRWLGGLP
                     PDVARKVAWDNGARLFGLPAP"
     gene            complement(7198..7881)
                     /locus_tag="PKX82_00610"
     CDS             complement(7198..7881)
                     /locus_tag="PKX82_00610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOF50772.1"
                     /translation="MGQFVQLTAADGFVFPAYEARPAGSPKGAVVVLQEIFGVNSHIR
                     AVVDGYAAQGYLALAPATFHRVKADVELGYGPDDIAAGRELKAAVEGLPAPGVLPDIA
                     AAVAHATQGGKVAVMGYCWGGLLAWRSACLLPGISAGVTYYGGGMTEPAEAARKPLCP
                     VLAHFGEQDHAIPLPGVHAFRAAQPGVQVELYAAQHGFNCDHRGAYNAAAAALAWQRT
                     LAFLAQHVG"
     gene            complement(7911..8894)
                     /locus_tag="PKX82_00615"
     CDS             complement(7911..8894)
                     /locus_tag="PKX82_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HOF50773.1"
                     /translation="MKFSVQKVWGVLRHTAVMGALLACGLAQAQTGPVKLMVGFPPGG
                     GTDAIARTLADKLKDQLGMPVVVENKAGAGGQLAAQALKAAAPDGMTLFLTHDHTISI
                     LPQVVKNPGFDPAKDFVPVAGFASFVNALAVSGGTPAKSVSEYVAWVKANGGGKGTVG
                     VPAPASTPEFLVGVIGKKYGLDLQAVPYRGSAPMMADMLGNQIAAGTGSVPDFIENHK
                     AGKIRVVAVLGGARQATLPDVPTFAELGLKGFEDMPYYGIFAPVGTPKATIDKFSDAL
                     AKVVAMPEVKDRLTAMGLTVGFMTQQQLATREQAYTQVWTKLIKDSGFQAK"
     gene            complement(8932..9696)
                     /locus_tag="PKX82_00620"
     CDS             complement(8932..9696)
                     /locus_tag="PKX82_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOF50774.1"
                     /translation="MGYRHAMLTTDAFAAYWSGPMLVANFFILLHLVGALVLGMLVGY
                     ERSYHGRAAGMRTYGLVCMAAAAVTVIGGYAHFWYGGATNAAFSNADLSRVVQGILTG
                     IGFLCAGVIMKEGFNISGLTTAASLWSSSVIGILVGVGLYAAAMALALLSALCMVWVS
                     KLEGWLPSRQAVAIALKFNPGFLPVEAALRGMANARGYDIAGGTIVISLDQGAYNWNF
                     VAVARSRQKMAPISDLGTEMGKFPGVQSFHIAYARN"
     gene            complement(9719..10819)
                     /locus_tag="PKX82_00625"
     CDS             complement(9719..10819)
                     /locus_tag="PKX82_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOF50775.1"
                     /translation="MKTTRNVGWERNVDFASLIEQYGPATVLSVGAAVIGVVFGFAAQ
                     RSRFCARAAVIETAQGQWGPRFCVWFLAFASALVAVQALVLAGGLDRGATRFIGQPGS
                     VSGAIVGGLLFGAGMILTRGCASRLLILSANGNLRALLSGLVFAVTVQATIAGWLNPL
                     RQWIAAWWPIDPGPSRDLLALTGTGAVGALVVSALVWGAAVLMCWKGPLRSRWAALGG
                     MVTGLMIAAGWALTQVVARNSFDPVVVQSLSFSGPSAEWLMRVLSSATAPGFGFDAAL
                     LPAVFAGSLLAALVFGEFRLEGFKTENRLGHYLLGAVLMGFGAVLAGGCTVGAGMTGG
                     AIFALTAWLTLISIWVGATVAFRVSRSLGLAT"
     gene            10868..11614
                     /locus_tag="PKX82_00630"
     CDS             10868..11614
                     /locus_tag="PKX82_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF50776.1"
                     /translation="MKPIALITGGSRGIGAATALAAARAGYAVAVNYHANSLAADEVV
                     RQIRAEGGMAMAVQADVAVEREVVEMFARVDAKMGRLSVLVNNAGVVDTTTRVETMDW
                     ARLRRMFDTNVLGSIVCAREAVLRMSTRHGGSGGTIVNLSSVAATLGGAGQYVDYAAS
                     KGAIDTFTVGLAREVAAEGIRVNAVRPGLIDTDIHASGGQPDRARALSPLVPMQRPGT
                     AEEIAQAILWLMGPESSYTTGALLDVGGGR"
     gene            11627..12259
                     /locus_tag="PKX82_00635"
     CDS             11627..12259
                     /locus_tag="PKX82_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOF50777.1"
                     /translation="MDLKPLVTLLAIVNPLAIVPFFIHYTQGFNAAQRRKTVLISSFS
                     SFVVIAVSALMGLHILEFFGISLASFQVGGGMLLLTSALNMLNAQPAEAKTNTHELED
                     GVEKAAMGASIAVVPLTIPLLTGPATMSTVVIYADKAKTFLQLGTLVGYGVVVALATA
                     LCFSAAQPIARVLGKTGINVMTRLMGLILAALAVEVMSDGLTKLFPALGH"
     gene            complement(12267..12851)
                     /locus_tag="PKX82_00640"
     CDS             complement(12267..12851)
                     /locus_tag="PKX82_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOF50778.1"
                     /translation="MLGLPGCGVLQTPARAVVYDFGPGAVVSPAASAGTPLPVLVLAD
                     VESPAALDTTAVLYRLNYSDAQQLRPYAQARWSMPPAQLVRQRMRETLGQQRAVLPQA
                     DGVAPRAAALTLRVALEEFSQLFEAADRSTGLVRVRATLSQGGAGRDQLVAQRSFVVQ
                     RDSTSADAAGGVRALTAATDAVIADIDQWVRQVP"
     gene            complement(12936..13931)
                     /locus_tag="PKX82_00645"
     CDS             complement(12936..13931)
                     /locus_tag="PKX82_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOF50779.1"
                     /translation="MENKSHAVAAGAFVLAIAALLVAMAAWLMRDTSEQRVFEISSRD
                     GVTGLQPQAGVRYKGVLVGRVTSIELDAQTRGSVLVRIAVNETAPITTSTYASLGFQG
                     VTGLAFIQLDDAGDSTQMLTTSKTQVTRIPMRPGMVSRFTSQGGQLLDQLEQASGRAN
                     ALLAADNQKALMAAIHNMGQAAASIQQLTQRADQMLGPAAGGEGMNLPRLAAQTEATL
                     KSMQATSERLGASADAVKTSATEFKQMSARMNAPGGTLDRIAQSTEALTATGQSLNAT
                     LVPRLNRTADEATRTVRQMGRVADAVNETPQSLILGRGAAQPGPGEPGFSAPARR"
     gene            complement(13944..14726)
                     /locus_tag="PKX82_00650"
     CDS             complement(13944..14726)
                     /locus_tag="PKX82_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF50780.1"
                     /translation="MSAVQIDKLWSTFRTNGVDAVIHKDLDLTIAEGEFLSVVGGSGS
                     GKTVLLRQILGLETPTRGSVTVLDRPAAELGRSGAASRVGMLFQHGALFSAFTVLENI
                     AFPLRELGTLPPELVRDVAMVKLQMVGLDPSHANKMPADLSGGMVKRVALARALVMDP
                     PLLLLDEPTAGLDPDLSDAFCALLRSLHQELGLTVVMVTHDLDTIYELSTRIAVVADK
                     HVIVNATPREVVAFPHPFVHDFFLGGRGHRAMEVLRSSPQGV"
BASE COUNT         2966 a         4960 c         4477 g         2406 t
ORIGIN      
        1 caccccgctg acccgcctca cccgggacta ccccggcctg ctccaccccg tcgagggcaa
       61 ccagcggcac atccaccacg gtttgctcac ccgaagtgac acgcagggtt cccgcaatct
      121 ggcccttggc aatcggcgcc accagcgggt cgttgcgcac cacctgcgtt ttcagcttgg
      181 cggcagagcc cgcgggcacg accacaacga tgggctggtt gcgtccgagg cgcaccgtcg
      241 aagccgcacc cttccagaca ttgggggtca ccaccgcctg gtctgcatca aacagcttga
      301 ctggctcaaa agccacaaat ccccagttga gcaacttctg ggactcgttg gcacgggcat
      361 tctcattggc cgtgcccagc acaatggaca gcaaccgccg gcttccgccg gctggagaaa
      421 tctcgcgttt ggcagtcgcc accatgcaat agccggcagc atcggtgtaa ccggtcttca
      481 ggccatccac agtcgggtca cgaaacagca atagattgcg attgctgtcg ttcgcagccg
      541 gggtgcccgg ataacggtat ttcttgatcg cgtagaaccc gacgtaatcg gggaagtctg
      601 ccatcagccg cgttgccaga atgctcagat cacgggcggt ggtggtgtgc cccgcctcgg
      661 tcaagccctc gggatttctg tacccggtcg atttcattcc cagggccttg gcctgctcgt
      721 tcatcatctg aacaaaccgt tctgcggtac cgcccacacc ttcggccaat gccatggtgg
      781 catcgttgcc ggactggaca atcatcccct tgatgaggtc ctccaccggg acctgcattt
      841 tcggatcaat gaacatccgg gagccaggca tcttccaggc ccgctcactg accggcaaag
      901 cctgcttcaa atccagcttt ttggcccgca gggcatcaaa caccagataa gccgtcatca
      961 gcttggtcag ggaggcgggc tccaggggcg catcgatatc cttggcggcc agaatctggt
     1021 tcgccgtcac atccagcagc agataggacc gcgccgcgat ctcgggtggt tggggagttt
     1081 gcgcagaggc ggcaaaacac aggcaggcca agaaggccga aaacaagaat ttcatgggga
     1141 agatgaggag ctaggcaaag ggagcgctga gatggcgaac caccagattg cgcaaaagag
     1201 gcaattgtcc atgaaagaag tgctctcctc caggaatcac cgtcaccggc agactctggg
     1261 gccgcgccca gtccatcacg tcgaccaatg gaacagtctc gtccacctct ccatggacca
     1321 ccagggttcg gtcatgcaat accggtgcaa tgggggcaac ctgaaaccgc gttgcagccg
     1381 ttccgaccag aaccagcttt tctacaaccc gtgtttgtgc caatgcctgc gcagcatggc
     1441 tggcaacaaa cgaaccgaaa gaaaaaccgg cgacagccaa agaaccctca ggagcgaagt
     1501 gtgcaaccac tgacaaatag tccaacaatt cgccgcgccc ctcgtcatac gctccctggc
     1561 ttttgccgac accgcgaaaa ttgaaccgca cggcgcgcca gccacacagg acaaaagccc
     1621 gtgccagggt ttgcaccacc ttgttgtcca tggttccgcc aaacagaggg tgcgggtggg
     1681 cgatgactgc cgttccgcgc ggcacgccca gcgctggtgc cggctcatcc agcagcacct
     1741 ccatcaggcc ggcagtgccg tcgatggtca tgcgttgcgt ctgcgaattc atgcagtcac
     1801 ccttgcaagc aatagcgtct gctgcaccta gcgacccaga tggggcggcg tcagcaaccg
     1861 ctccaccacc tgaccatttt tcaagtggga ctccacgatt tcgtcaatat cctgcaagtc
     1921 cacataggtg taccagatgg cctcggggta gaccacggcc accggtcctg cggcacagcg
     1981 gtcaaggcag ccggccttgt tgacgcgcac cttgccggga ccagccagtc ccgcagcttt
     2041 cacctggccc ttgcaccggt cgaaagcttc ctgcgcctgg tgctgcgcac agcactcttc
     2101 gccgtttttg cgttcgttga ggcaaaagaa aatgtgtcgc tcgtagtagg agcctggagt
     2161 gcgtgtggaa tcagtcatgg tgcgattcta gtttttcggg ttgcgctgcc cgacccggac
     2221 caccacatac aacagagccg catagggcca aagccagccc agccattggg ccaacccgtg
     2281 aaaccggatg aaccgcccct gctcccagtc ctgcaatgtt tgcgcgaagt acgcactttc
     2341 gggcgcctgg ttgagcaggc tcaggtagac ccccagcgcc agcagggcca gcgcagcact
     2401 gacccgccag gaagccagtg tggcgaccag tgcaagaacg atggcaccgg caattcccag
     2461 ttgcgtgggc aggtccagcc acgaccaggc atgaacaggc ccccaactca gtgccgcgga
     2521 cagcccggtc accagcacgc cgacaccaat gcacagcggc gcaaaaacca gacgccgcca
     2581 ggtcgagcgg atcacgcagt atcccagcag gcacgggatg aggagcccca ggagcacgca
     2641 gaccatctcc gcaccgggaa ccagcggctg caattcgatg tcgcgcaccg gcatccagtc
     2701 cagaaacggc gtatctgcca gacggtccgc gatggccgcc tcaatacgct cgaaaacctg
     2761 tccgagccca aaaggtacgg cggccggaaa cagcagcgcc aaaggccagg ttgccagaag
     2821 taccagccca ccgcgggcat gcggaacaaa ccagcgggca cgaaaccggc tccaccggtc
     2881 aatcgccccc atgcgctcca gcagtacggt ggcggctgcg cccagcgctg cgcccagggt
     2941 attgagcagc cagtcctcgc gcgaaggaac ccgtaggggc aaatagctct gcaacgcctc
     3001 catcgcaaag gacagcacac ccgcaagcac gaccggaagc cacagcgcct gacggctgcg
     3061 gccggtccgc aatgcgctca acgccagcat gctccccagc ggaaggtaac ccgccacatt
     3121 gatggccaca tcaaaccgcg tccagtagcg cgggaacggc gccagcagaa acgaccacgg
     3181 caggatgtcc tggtcacgcc attcggcaaa cgggtacagg ctggcataca ccaccagcac
     3241 cgcatacatc agcgccaggg gtgaagcagc ggttttgtgc atggctaccc ggtcagaagg
     3301 gtttgaccac aaccagaacg acgatggcca ccagcatcag caccggcgct tcattgaacc
     3361 agcggaacca cacatggccg cgcgatgcaa cacccgcttc aaacttgcgt agcagcctgc
     3421 cacagacgtg gtggtagccc agcaccagca ccaccagcag aagcttggcg tgcatccagc
     3481 cgttgcccgg cccccatccg atgccataac ctatccagag ccacagcccc agacccacgg
     3541 ctggaacggc cagcagggta gtaaagcgca tcagcttgcg cgccatcagc agcaggcgtg
     3601 cgcgttccgc ggtggagtcc gcgggcacca tggccagatt gacaaaaatc cgcggcaagt
     3661 agaacaggcc cgcaaaccag ctggccacga acacgatatg gaaagatttg acccagagca
     3721 tggctggagt gtagccagcg ttcaggccac aagctgcttg cagcttgcgt cgtaggctaa
     3781 ctttgcgcag caaagttaag cgcagcggcc gaagacaaaa aaaaccccgg tacaggaccg
     3841 gggttggaag cctatttgct gtcacacatc aaggccccgc tcagggagga aaagcgggag
     3901 gtagcaagct acctggggag gaatatagca actttgaata cccgctgcaa gaaaccggaa
     3961 tttttttctg catttttata tgccatttgc ctgtcggcgg gctatcaggc agggggtatg
     4021 ctgcaacgcc accaacggag gtttctaccc gctgccggga atcaggcaca attttcccca
     4081 tgacatacac caccccctac gcccccttcc cggcgggccg cccccgccgg ctgcgccggg
     4141 acacgttcac ccgcaacctg gtccgtgaga acacactgac cgcccacgac ctgatctacc
     4201 ccgtctttgt cgtggaaggt cagggccaac gcgtgcccat cagctccatg cccggcgtgg
     4261 agcgcctgag cctggacctg ctgttgcccg tggccgaaga atgcgtgaag ctggaaatcc
     4321 cggtgatggc gctgtttccg gtgattgacc agtcgctgaa aaattacgag ggctccgaag
     4381 cactcaatcc ggacggcctg gtgccacggg tggtgcgcgc actgaagaaa gaatttcccc
     4441 agctcggcgt catgaccgat gtggcgctgg accccttcac cagccatggc caggacggcc
     4501 tgcccgacac gcgccctgaa accgagggct acatcctcaa cgacgaaacc gtggccatgc
     4561 tggtgcagca ggccatgacc caggccgatg ccggtgtgga catcgtcgcg cccagcgaca
     4621 tgatggacgg gcgcatcgcc gccatccgta ccgcgctgga agccaaaggc cacattcaca
     4681 cccgcatcat ggcctacagc gccaaatacg cgtccgcttt ctacggcccg ttccgcgacg
     4741 ccgtgggctc ggccagcaac ctgggcaagg gcaacaagaa ggtctaccag atggaccccg
     4801 ccaacaccga cgaggccctg cgtgaagtgg ccatggacat tgccgaaggt gccgacatgg
     4861 tgatggtcaa gccaggcatg ccttacctgg acgtggtgcg ccgggtgaaa gacgaattca
     4921 aggttcccac ctttgcctac caggtgtcgg gcgaatacgc catgctcaag gcagctgcgc
     4981 agaacgggtg gcttgaccac gatgcggtga tgatggaaag cctgctggcg ttcaagcgcg
     5041 ccggggccga cggcgtcctt acctactttg cacgcgacgc ggccaagttg ctccgaaaat
     5101 aatagctact cacgcaatca acacgagggc caggtgccaa tttggcatat aaccccaaac
     5161 aggaacccga tgcgcatatt ccatatccag agcagcactg tgaccgagag cgcggacatt
     5221 gacgccctcg ccaccagtgg cccgcccgcg cagggctata tctgggtggc ctgcgctcgg
     5281 gaagagttgg aatcgagtct cggcaaggtc cagtccatgt tgcagtcact gggcggcgca
     5341 cagctggtgg acctgcatgt gtccgacctg ctgaaccagc aactgccctc ccactacgac
     5401 tacacctcgc agtacgacat gctggtgttt cgccggctgg cggcgggcaa cggctctaca
     5461 ccgggcacgc gcatccccgc cagagggggg ccgcccatct tgcggcgcat tgataccagc
     5521 ccggtgggtt ttgtagtgct cgaccgcgtg ctcctcacgg tgcacccgac cgactgtgcg
     5581 gcgcgcgaag cctatgcggc caagctgctg caggcggcca gcgcagaatc ccgcgcggcg
     5641 ggtgcgcgcc tgcccaccag cccggccgac ctgatgctgc gcatcgtcaa ccagatggtc
     5701 gacagctatc tggccctgcg ccgggagctg acccaccagc tggaccactg gcaatccgag
     5761 ctgctgaacc ccaagacccg cttcaacaac tggtcgaccc tgatggacgc acggctggcg
     5821 ctgcaccatc tggacgagat ttgtgaagaa cagcgctcct ccatccagga ctggattgaa
     5881 gccgtcaaaa cctggcccga cgccaccacg gtggcggacc agcgcgaacg cgaactgctg
     5941 caggtccgca gccgcgatgt gctggaacac atcgaacgcg tggtccacca tgtgcgtcgc
     6001 ctggagcaaa gtgcagaaac cgcagtgcag atgcacttca gcgcgcagag ccaccgcacc
     6061 aacgacatca tgcgcacgtt gacggcactc accgccatct ttctgccact gaacctgatt
     6121 gccggcatct tcggcatgaa cttcgacttc attcccctgc tgcacatcca cagcggcttc
     6181 tggtgggcca tgggcagcat ggccctgatt gccatggcgc tggggttgct gttctggcgc
     6241 aagcgctaca tcgcgcgctc ggagcactag gctgcgtgcg cgtcagggcg cgggcagccc
     6301 gaacagccgc gcgccgttgt cccaggccac cttgcgcgcc acgtcgggcg gcaggccgcc
     6361 gagccagcgt cggtacccct gcatgagctc gtcgtagtac agccagcgct ggttgaccca
     6421 ggtgtccgac ccgatcagaa agcggtcagg gtatttggtg accagcgcac gccactcggg
     6481 gcacagctgg ccgtcgtcgc aggtcaggcc ggggcggtat gacagttcac ccatcaacag
     6541 cgggtagcgc gccatcaggg catccacccg tgcggcgggc gcaccgccga tgccggtgtg
     6601 cgcccagatc agtcgtgctt tgggcgcgtg ggccatcagc aggtcaatgg ccacgtcgtc
     6661 cacatgcgcc agcaccgcca gttgctgctt ctcggccagc tgcatcagct tgcgggctac
     6721 cgggccgttg gcattggcgc tgtcgtacag gtggaattcg cccaggccct tgtaggggcc
     6781 ggcacccgtg ccacgcgcca gctcggtttg caccagggtg tagatggttt catcgcgaaa
     6841 ccagttgtcg taatcggcgc ggttgcggta caggcgcaca aagggcacca cggtgacgcc
     6901 agcggcgcgg gtctgcgggg catcggccag cgccttggtg ccgtcgttgg gccggctgtt
     6961 ggcgacgatg gcacgcacac cgttgcgctg catgcgcgcc agcacatcgg gcaacgggtg
     7021 cggcccggtt tgaccgttcc aggcttcttc gttgtagtgc aggtgggcgt ccagcagcgg
     7081 gccggtgtag tcagccgccc aggcagacgc cagacttgct atacaaataa tagctgcttg
     7141 cgcatagcgg acgggggcca gaggccaatt tggcataaat tcccctgtgt caatgcccta
     7201 accgacatgc tgcgccagaa aggccagcgt gcgctgccat gccagtgccg cagctgcggc
     7261 gttgtaggca ccgcggtggt cgcagttgaa gccgtgctgc gcagcgtaca gctccacctg
     7321 cacgccgggc tgcgccgccc ggaacgcgtg cacacccggc aggggaatgg cgtggtcctg
     7381 ctcgccaaag tgcgccagca ccggacacag cggcttgcgc gcggcttcgg ccggttcggt
     7441 catgccgcca ccgtagtagg tcacccccgc gctgatgccg ggcagcagac aggccgagcg
     7501 ccaggccagc aggccacccc agcagtagcc catgacggcc accttgccgc cctgggttgc
     7561 atgggccacg gccgcagcaa tgtcaggcag cacacccggc gcgggcaggc cctcgaccgc
     7621 agccttgagt tcacggcccg cagcgatgtc gtccgggccg tagcccagct ccacgtcggc
     7681 cttcacccga tgaaaggtcg ccggcgccag cgccagatag ccttgggcgg catagccgtc
     7741 caccactgcg cggatgtggc tgttgacgcc aaagatctcc tgcaacacca ccacggcgcc
     7801 cttcgggcta cccgcgggcc gggcttcata ggcgggaaag acaaagccat ctgccgcagt
     7861 gagttgaacg aactggccca tggtgccttc agatgcgcgg atgcttgtgt ttacttggcc
     7921 tggaagccgc tgtccttgat cagcttggtc cacacctggg tgtaagcctg ctcacgggtg
     7981 gcgagctgct gctgcgtcat gaaacccacg gtcaggccca tcgcggtgag gcggtctttc
     8041 acttcgggca tggccaccac tttggccagc gcgtcggaga acttgtcgat ggtggccttg
     8101 ggggttccga ccggggcgaa gatgccgtag tacggcatgt cttcaaaacc cttgaggccc
     8161 agctcggcaa acgtcggcac atcgggcagg gtcgcctggc gggcaccacc cagtaccgcc
     8221 acgacgcgga tcttgccggc cttgtggttc tcgataaagt cgggcaccga accggtacct
     8281 gccgcaatct ggttacccag catgtcggcc atcatggggg cactgccacg gtagggtacg
     8341 gcctgcaggt ccaggccata tttcttgcca atcacaccga ccagaaactc gggtgtggag
     8401 gccggggcag gaacaccgac ggtacccttg ccgcctccat tggccttgac ccaggccacg
     8461 tactcactga ccgacttggc aggcgtgcca cccgacactg ccagcgcatt cacgaacgag
     8521 gcaaagccag ccaccggcac aaagtccttg gccggatcga agcctgggtt cttgaccacc
     8581 tgcggcagga tcgaaatggt gtggtcatgg gtcagaaaca gcgtcatgcc atcgggggct
     8641 gccgccttga gcgcctgggc tgccagctgg ccaccggcac ctgccttgtt ctccaccacc
     8701 accggcatgc cgagctggtc tttgagcttg tcggccaggg tgcgggcaat ggcgtcggtt
     8761 ccgccgccgg gggggaaacc taccatcagc ttgaccgggc ccgtctgggc ctgcgccagg
     8821 ccacaggcca gcagggcacc catcaccgcc gtgtggcgca gtacacccca aactttttgc
     8881 acagaaaact tcattgcaat cactcctgga aaagaaacac caaaaacaaa actagttgcg
     8941 cgcgtaggcg atgtggaaac tctgcacacc ggggaacttg cccatctccg tacccaggtc
     9001 ggaaatgggg gccatcttct gccggctgcg ggccaccgcc acaaaattcc agttgtaggc
     9061 accctggtcc agggaaatga cgatggtgcc gcccgcgatg tcatagcccc gcgcgttggc
     9121 catgccccgc aaagccgcct ccacaggcag gaaacccgga ttgaacttga gcgcgatggc
     9181 caccgcctga cgcgagggca gccacccttc cagcttcgac acccacacca tgcacagggc
     9241 gcttaacagg gccagcgcca tcgccgccgc gtacagcccc accccgacca ggatgccaat
     9301 caccgacgaa gaccacagcg aggccgccgt ggtcaggccg ctgatgttga agccttcttt
     9361 catgatgacg cccgcgcaca gaaaaccgat gcccgtcaga atcccctgca ccacacgcga
     9421 caggtccgca ttggaaaacg ctgcattggt ggcaccgccg taccaaaaat gcgcatagcc
     9481 accgatgaca gttaccgcag ccgccgccat gcagaccagc ccgtaagtgc gcatccccgc
     9541 tgcgcgcccg tggtaggagc gttcgtaccc caccagcatg cccagcacca gtgcgcccac
     9601 caggtgcaac aggatgaaga agttggccac cagcatgggg ccagaccagt aggcagcgaa
     9661 agcgtccgtc gtcagcatgg cgtgacgata acccatttgt tctgcgcagg acaggctctc
     9721 aggtggccag accgaggctg cggctgaccc ggaacgccac ggtggccccc acccagatgg
     9781 aaatcagggt cagccaggcc gtcagggcga agattgcccc ccctgtcata cccgcaccga
     9841 ccgtacagcc tcccgccagt accgcaccaa agcccatcag caccgcaccc aggaggtagt
     9901 ggcccaaacg gttttccgtc ttgaagcctt ccaaccggaa ctcgccaaac accaacgcag
     9961 caagcagcga tccggcaaac acggcaggca gcagggccgc atcgaacccg aaacccgggg
    10021 cggtggcgct ggacaaaacg cgcatgagcc attcggccga ggggccgctg aagctcaggc
    10081 tctgcaccac caccggatca aaggaattgc gcgccaccac ctgggtcaac gcccatccgg
    10141 cggcaatcat cagtccggtc accatgccgc ccagggccgc ccagcggctg cgcaggggcc
    10201 ctttccagca catcagcacg gcggcacccc acaccagcgc actcaccacc agcgcgccca
    10261 cggcccccgt gccggtcagg gccagcaaat cgcgcgatgg gcccgggtca atcggccacc
    10321 aggcagcgat ccactggcgc agcggattga gccagccggc gatcgtggcc tggaccgtca
    10381 cggcaaaaac cagcccggac agcagggcac gcaggttgcc gttggccgag agaatcagca
    10441 gccggctggc acagccgcgc gtcaggatca tgccggcccc gaacagcagc ccacccacaa
    10501 tggcacccga cacgctgccc ggctgcccga tgaagcgcgt cgcgccccgg tccagtccac
    10561 cggcaagcac cagcgcctgc actgccacca gggcactcgc aaacgccaga aaccagacac
    10621 agaagcgagg gccccactgg ccttgcgcgg tctcgatcac cgcagcgcgc gcgcagaagc
    10681 gcgaacgctg cgcggcaaag ccgaacacca caccaatcac ggcggccccc accgacagca
    10741 cagtcgcagg gccgtactgt tcaatcaggc ttgcaaaatc cacattgcgc tcccatccaa
    10801 cgttgcgggt tgttttcaac aaagcgcgta ttgtccattt tttggcacac gcgctaccat
    10861 gcctcccatg aaacccattg ccctgattac tggtggctcc cgcggtattg gcgcggcgac
    10921 ggctttggca gccgcgcgcg cgggctatgc cgtcgcggtg aactaccacg ccaactccct
    10981 tgcagccgat gaagtggtgc gccagatccg cgccgaaggc ggcatggcca tggcagtgca
    11041 ggccgatgtg gcggtagagc gcgaagtggt cgagatgttc gcccgagtcg acgccaagat
    11101 gggccgcctc agcgtgctgg tcaacaacgc gggggtcgtg gacaccacga cccgggtcga
    11161 gaccatggat tgggcacggc tgcggcgcat gttcgatacc aatgtgctgg gcagcattgt
    11221 ctgtgcgcgg gaggcggtgc tgcgcatgag tacccgccat ggcggcagcg gcggcaccat
    11281 cgtgaacctg tccagcgtcg cggccacact gggcggcgcg ggacagtatg tggactatgc
    11341 ggccagcaag ggggccatcg acaccttcac cgtgggtctg gccagggaag tagcggccga
    11401 gggtattcgc gtcaatgcgg tgcgcccggg cctgatcgac accgacattc atgcttcagg
    11461 cggtcaaccc gaccgtgccc gcgcactgtc cccgctggtt cccatgcagc gcccgggcac
    11521 ggcggaagaa attgcgcagg ccattctctg gctcatggga ccagaatcaa gttacaccac
    11581 tggcgcactg ctggatgtgg gcggtggacg ttaaggaaac caagctatgg atctcaaacc
    11641 cctcgtcacc ctgctggcca tcgtcaaccc gctggccatc gtcccgttct ttatccacta
    11701 cacccagggg ttcaatgcgg cgcagcggcg caagacggtg ctcatctctt cgttcagcag
    11761 ctttgtcgtg attgcggtca gcgcgctcat ggggctgcac atcctggaat tcttcggcat
    11821 ttcgctggcc agctttcagg tcggcggcgg catgctgctg ctaacttcgg cgctcaacat
    11881 gctcaacgcc cagccagcgg aagccaaaac caatacccac gaactcgaag acggagtcga
    11941 gaaagctgcc atgggcgcca gcatcgcggt ggttcccctg accatccccc tgctgaccgg
    12001 accggccacc atgtcgaccg tggtgatcta tgcggacaag gccaagacct tcctgcaact
    12061 cggcaccctg gtgggctacg gcgtggtggt ggcgctggcc acggcgctgt gtttctcggc
    12121 cgcccaaccc attgcgcggg tactgggcaa gaccggcatc aatgtcatga cgcgtctgat
    12181 gggactgatc ctggccgcgt tggcggtaga agtgatgtcc gacggattga ccaagctgtt
    12241 cccggccctg ggacactaga aagcgttcag gggacctgcc gcacccactg gtcgatgtcg
    12301 gcaatcacgg cgtcggtcgc cgcggtcaat gcccgcacgc caccggcagc atcggcactg
    12361 gtactgtcgc gctgcacgac aaagctgcgc tgggccacca gctgatcacg ccctgcacca
    12421 ccctggctca gggtggcacg cacacggacc agaccggtgc tgcggtccgc tgcctcgaac
    12481 agttggctga actcttccag tgccacccgc aaggtcagcg ccgcagcgcg gggtgccacg
    12541 ccatcggctt gcggcaggac ggcacgttgc tgccccaggg tctcgcgcat ccgttgccgc
    12601 accagctgcg cgggcggcat gctccagcgg gcctgggcat aggggcgcag ttgctgcgca
    12661 tcgctgtagt tcagacggta gagcacggcc gtggtgtcca gtgccgcagg cgattccacg
    12721 tccgccagaa ccagtacggg caggggggtg cctgcactgg cggcaggcga cacaacggca
    12781 cccgggccaa agtcgtacac caccgcgcgc gccggcgtct gcagcacacc gcagcctggc
    12841 aggcccagca ttaccaaggc aaataggcac ccagcccccg tcaatctcgc gtagttagct
    12901 atcatttttg tagtgtctat tgcaaatgtc cgtggtcagc gacgcgcagg cgcagaaaac
    12961 ccgggctccc cgggccccgg ctgcgcggca ccccggccca gtatcaggga ttgtggagtc
    13021 tcgttcaccg catcggccac acgccccatc tggcgcacgg tgcgggtggc ctcatcggcg
    13081 gtgcggttga ggcgcggcac cagcgtggcg ttgagggact gtccggtcgc agtcagcgcc
    13141 tcggtgcttt gcgcgatccg gtccaaagtg ccgccaggcg cattcatgcg ggccgacatc
    13201 tgcttgaatt ccgtagcgga tgttttcacc gcgtcggcac tggcgcccag gcgctccgac
    13261 gtggcctgca tgctcttgag cgtggcctcg gtctgcgcag ccaggcgcgg aagattcatg
    13321 ccctccccac ccgcagcggg gcccagcatc tggtcagccc gctgggtcag ctgctggatg
    13381 ctggctgcag cctgtcccat gttatggatc gctgccatca aggctttctg gttgtccgcc
    13441 gccagcaggg cattggcccg gccgctggcc tgctccagct ggtcgagcaa ttgcccgcct
    13501 tgcgaggtga agcgcgacac catgcccggg cgcatgggaa tgcgggtgac ctgggttttg
    13561 ctggtggtca gcatctgggt ggaatcgccc gcatcgtcga gctggatgaa ggccagcccg
    13621 gtgacgccct gaaaacccag cgacgcgtag gtggacgtgg tgatgggggc agtctcattc
    13681 accgcgatgc gcaccagcac gcttccgcgt gtctgggcat cgagttcaat gcttgtcacc
    13741 cgcccaacca gaaccccctt gtagcgcacg ccggcctggg gctgcaagcc ggtcacaccg
    13801 tcacggctgg aaatctcgaa gacgcgttgc tccgacgtgt ctcgcatcag ccatgccgcc
    13861 atggccacga gcagggctgc gatggccaga acgaaggctc cggcggccac agcgtgggat
    13921 ttgttttcca taaggttctc tttctacacg ccctgggggg aactgcgcaa cacttccatg
    13981 gcacggtgac cccgcccacc caggaaaaag tcgtgcacaa acggatgcgg gaacgccacc
    14041 acctcacgcg gcgtagcgtt cacgatgaca tgtttgtcag ccaccacagc gatgcgagtg
    14101 ctcaactcgt aaatggtatc caggtcgtgc gtgaccatca ccacggtcag gcccagctcc
    14161 tggtgcaaag accgcagcag cgcacagaag gcgtcggaca ggtccgggtc cagtccggcg
    14221 gtcggctcat cgagcagcag caggggcggg tccatcacca gtgcgcgggc cagtgcaacg
    14281 cgcttgacca tgccgccgga caggtcggcc ggcatcttgt tcgcatggga tggatcaagc
    14341 cccaccatct gcagcttgac catggccaca tcgcgcacca gctccggtgg cagggtaccc
    14401 agctcgcgca ggggaaaggc aatgttctcc agcaccgtga acgccgaaaa cagcgcgcca
    14461 tgctggaaca gcatacccac ccggctggcc gcaccgctgc gccccaactc ggccgcaggc
    14521 cggtccagta ccgtcacgct gccgcgcgtg ggcgtttcca gcccgaggat ctggcgcagc
    14581 agcacggtct tgccactgcc cgagccaccc accaccgaca ggaactcccc ctctgcaatg
    14641 gtcaggtcca ggtctttgtg aatgaccgca tccaccccgt tggtgcgaaa ggtggaccag
    14701 agtttgtcga tctgaacggc actcatatac ccacgctctt gaagacgacg gcaaacaggg
    14761 catcgaccag aatcacgatg gtgatggaag tgaccaccga cgctgtggt
//
LOCUS       DAOSJM010000008        14408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4296_length_14408_cov_4.932349, whole genome shotgun sequence.
ACCESSION   DAOSJM010000008 DAOSJM010000000
VERSION     DAOSJM010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4296_length_14408_cov_4.932349"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKX82_00655"
     CDS             <1..228
                     /locus_tag="PKX82_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOF50781.1"
                     /translation="ASEAYWVENGKVQYPVKGATIVGNGPDALTRVTMIGNNMKLDSG
                     VGTCGKEGQSVPVGVGQPTLRIDGLTVGGTA"
     gene            448..1584
                     /locus_tag="PKX82_00660"
     CDS             448..1584
                     /locus_tag="PKX82_00660"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOF50782.1"
                     /translation="MTAKASATGDWNGQMHPADRTSKTDDKRIKDITVLPPPEHLIRF
                     FPIRGTAVENLIADTRKSIHNIMAGKDDRLLVIIGPCSIHDPAAALDYARRLKEQREK
                     YKDTLEIVMRVYFEKPRTTVGWKGLINDPYLDESFRIDEGLRIARQLLIEINRLGLPA
                     GSEFLDVISPQYLGDLISWGAIGARTTESQVHRELASGLSAPIGFKNGTDGNIKIATD
                     AIQAAAGGHHFLSVHKNGQVAIVQTNGNPDCHVILRGGKAPNYDAASVAAACADLAKA
                     KLPETLMVDCSHANSSKQHEKQLDVAKDIAIQISGGSQRVFGVMVESHLNAGAQKFTP
                     GKDDSAKLEYGRSITDACLGWDDSVASLDVLAQAVKARRTRKPA"
     gene            complement(1592..2080)
                     /locus_tag="PKX82_00665"
     CDS             complement(1592..2080)
                     /locus_tag="PKX82_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50783.1"
                     /translation="MPDTKTAKLSTTQIAGNGPFTVHGIVEISMDGPLMHYQGRGPFN
                     KELVGLMGIAQRDYLLSIRPQGNWVSVCTMVESAMTSPEGLARYAEIMASPKPDNMVP
                     IATAFVIGPEIEGGRLMAPLYAKIYADIGRPFALFETMPEALKWAQAMIDAASTGKPA
                     PT"
     gene            complement(2110..3582)
                     /gene="mpl"
                     /locus_tag="PKX82_00670"
     CDS             complement(2110..3582)
                     /gene="mpl"
                     /locus_tag="PKX82_00670"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HOF50784.1"
                     /translation="MHIHILGICGTFMGGLAALAREAGHRVTGCDAGVYPPMSDQLRA
                     LGIELIEGFGADQMALQPDMFVIGNVVSRTRLSDGTPKFPLMEAILDAGAPYTSGPQW
                     LAEHVLQGRHVLAVAGTHGKTTTTAMLAWILESAGLQPGFLVGGVPMNFGISARLGGG
                     APAPSAQVKEEPAARAGDTEQKALAPHRPLFVIEADEYDTAFFDKRSKFVHYRPRTAV
                     LNNLEFDHADIFDNLAAIERQFHHLVRTVPGSGRVVVNGLEESLTRVLHQGCWSEVRS
                     FGAAVSDFTAVGEPHDFAVHYQGTPVAHVQWALGGVHNQLNALAAIAAAEHVGVSPAL
                     AAKALGTFENVKRRMEVRGQVTLRAGGAPVTVYDDFAHHPTAIRTTVNGLRRKAGAAR
                     ILAVFEPRSNTMKLGAMKAQLPWSLEEADLAFCHSGGLGWDATEALAPLGPKASVADT
                     IEQLTQQVMAQVRPGDHILCMSNGGFGGIHAKLVTLLQKA"
     gene            3654..4247
                     /locus_tag="PKX82_00675"
     CDS             3654..4247
                     /locus_tag="PKX82_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50785.1"
                     /translation="MDTIKEEIAATAARMVVEEGLEYGAAKRQATKQLGVSARSGLPD
                     NDQVEAAVREYIALFCADTQPGELRALRELALVWMERLERFRPHLAGAVWHGTATRRS
                     DIYIQLFCDDPKSAEIGLIDMGVRFEVTSVNGFQGYPVDALSLHLRCAPLGEYVGLHL
                     MVYDFDDLRGALKPDAQGRRPRGDLAAVRGLLHDVAP"
     gene            4244..4819
                     /locus_tag="PKX82_00680"
     CDS             4244..4819
                     /locus_tag="PKX82_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOF50786.1"
                     /translation="MSSDQNESANGAQARGHQRRMLVGAAVAATVAGAGIAMWRTRSD
                     AVAPARSEPVDGFWALQWEVPQGGDFMRMADFRGRPLLLNFWATWCPPCVEELPLINA
                     FYKENLANGWQVLGLAVDKASAVQAFLKKMPLEFPVGMAGIAGADLGRSLGNLAGGLP
                     FSVALSANGSVIQRKMGRLTASDLQAWARLK"
     gene            4992..5447
                     /gene="accB"
                     /locus_tag="PKX82_00685"
     CDS             4992..5447
                     /gene="accB"
                     /locus_tag="PKX82_00685"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOF50787.1"
                     /translation="MDLRKLKTLIDLVSDSNVSELEITEAEGKVRIVKGGGAVVQSYA
                     PAPVYAAQAPAVAAPAAAAAPAPVAEPVAEGHTVKSPMVGTFYRAASPGAKPFVEVGD
                     SIKVGETICIIEAMKILNEIEADKSGTVTRILCDNGQAVEYGQPLFIIE"
     gene            5465..6814
                     /gene="accC"
                     /locus_tag="PKX82_00690"
     CDS             5465..6814
                     /gene="accC"
                     /locus_tag="PKX82_00690"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOF50788.1"
                     /translation="MFKKILVANRGEIALRIQRACRELGVKAVMVYSEADREAKYVKL
                     AEEAVCIGPAPSGQSYLNMPAIISAAEVTDAEAIHPGYGFLSENADFAERVEKSGFQF
                     IGPAPESIRIMGDKVSAKQAMIRAGVPCVPGSDGELPDDPVQIRRIAKAVGYPVIIKA
                     AGGGGGRGMRVVHTEAALVNAVAMTKAEAGAAFNNPAVYMEKFLQNPRHIEIQIIADK
                     YKNAVYLGERDCSMQRRHQKILEESPAPGINRKLIERIGERCVAACKKIGYRGAGTFE
                     FLYEDGEFYFIEMNTRVQVEHPVTEMVTGIDIVKTQIMVAAGEKLPFTQRQIEIRGHA
                     MECRVNAEDPYKFTPSPGRVTMWHAPGGPGVRVDSHVYTNYFVPPNYDSMIGKIIVHG
                     DTREQAMARMRTALAETVVEGINTNIPLHRELMVDAKFMAGGTNIHYLEQWLDQHKR"
     gene            6820..7698
                     /gene="prmA"
                     /locus_tag="PKX82_00695"
     CDS             6820..7698
                     /gene="prmA"
                     /locus_tag="PKX82_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOF50789.1"
                     /translation="MFELRLMCPEDRVEGVSDALDALDALSVSVEDADAQTDAEQALF
                     GEPGMPPPKDGWQRSRIIALFASEALAREAAALLHAQDFFAGCQVLDVLSVPEQDWVR
                     LTQSQFAPVEVTPEFWIVPTWHEPPAQARQVIRLDPGLAFGTGTHPTTRMCLRWVATH
                     AVNGRVLDYGCGSGILAIGAAKYGAQEVVAVDIDEAAVQSTELNAAANEVQLSAGLPE
                     KAQGTFDVVLANILATPLKVLAPLLCAHVRKGGNLVLAGILARQEDELKEAYAPYCQL
                     TVADSEDGWILMTASV"
     gene            7734..8756
                     /locus_tag="PKX82_00700"
     CDS             7734..8756
                     /locus_tag="PKX82_00700"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HOF50790.1"
                     /translation="MSLITRCPACATMFKVVPDQLRVSDGWVRCGQCDEVFDANAHLQ
                     SPAEVESESVAPEDSSQLDWDGTLEEAAAPTSEIQLDFPDEVDRNSAHAVRPSAEDES
                     AVPDVNPEGPLDHLDGADAPPVAGDPLDAIPGDDVKDLQATAEDVQPVLPFVESADKD
                     LEGNGATPPGFLRKSHTGSRRSSRSTRVLGVTFCVLLVCSLGMQILVQERDRIVATES
                     ATLALLQPVCDVLGCRIAPLRQIESVAIDHSAFSKVQSDVYRFSFAIKSTALVAVAVP
                     SLELTLTDLQDRPVIRRVFSPRELGAQVEVVAPGGEWSGSVPVEVKMPDGAASIAGYR
                     MLAFYP"
     gene            complement(8810..9277)
                     /locus_tag="PKX82_00705"
     CDS             complement(8810..9277)
                     /locus_tag="PKX82_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HOF50791.1"
                     /translation="MATAKKPAAKKAAPAKKAAPAKKAAPAKKAAPAKKVAAKKAAPA
                     KKAAPAKKAAPAKKAAAAKKPAAKKAAPAKKVAAKKAAPAKKAAAKKAPAKKAAVAKK
                     PAAKKPAAKKAAKKPAAKKAAKAPAAKAAPAAPAAQTTLNPQAAWPFPTSSKP"
     gene            complement(9448..10641)
                     /locus_tag="PKX82_00710"
     CDS             complement(9448..10641)
                     /locus_tag="PKX82_00710"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HOF50792.1"
                     /translation="MLTWDDEVKPSTPTNLARTAAVNAPLVQESSSSLNLRTLDDGAL
                     VPSSSSINASPTVAPRRVRAADKRIINGQTDVNQLVPFKYKWAWEKYLATCANHWMPQ
                     EVNMTRDIALWKDPNGLTEDERRIVKRNLGFFVTADSLAANNIVLGTYRHITAPECRQ
                     FLLRQAFEEAIHTHAYQYIVESLGLDEGEIFNAYNEVQSIRDKDEFLIPFIEAIMNPN
                     FHTGTQETDQTLLKSLIVFACLMEGLFFYVGFTQILALGRQNKMTGAAEQYQYILRDE
                     SMHCNFGIDLINQLKAENPNLWTAEFKAEIKALFEKAVELEYRYAEDTMPRGVLGMNA
                     SMFKGYLRYIANRRATQIGLEALFPNEENPFPWMSEMIDLKKERNFFETRVIEYQSGG
                     ALSWD"
     gene            complement(10761..13697)
                     /locus_tag="PKX82_00715"
     CDS             complement(10761..13697)
                     /locus_tag="PKX82_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HOF50793.1"
                     /translation="MQSVSSAAPTPTTSSIAATNVPAFAGESTSPLAHYQIIRRNGAV
                     VPFEPNKIAVAMMKAFLAVHGTQGAASASVRETVDGLTQQVIRALMRSRPGGGTFHIE
                     DVQDQVELGLMRGGHHEIARAYVLYRERRTQERAKQVAQAAPAAPTLHVLEGGQRIAL
                     DLEQLKTRIEVACAGLGADVKADPIVAETMRNLYDGVPMDEVYKASILAARTLIEKDP
                     DYTYATARLLFHTISREVLGRDVAQADMGQAYCDYFPGFIKKGVDAELLDEKLLQFDL
                     KRLGAALKADRDLNFDYLGLQTLYDRYFLHVGKTRIELPQAFFMRVAMGLSLNEIDRE
                     ARAIEFYEVLSSFDFMSSTPTLFNSGTLRSQLSSCYLTTVPDDLDGIYESIKENALLS
                     KFAGGLGNDWTRVRALGSHIKGTNGESQGVVPFLKVVNDTAVAVNQGGKRKGAVCTYL
                     ETWHLDIEEFLELRKNTGDDRRRTHDMNTANWIPDLFMRRVMEKGTWTLFSPSNVPDL
                     HDLFGADFEKAYVAYEEKAARGDIKPARTIQAADLWRKMLTMLFETGHPWITFKDACN
                     VRSPQQHAGVVHSSNLCTEITLNTSDTETAVCNLGSVNLLQHLKDGANGKELDHDKLK
                     RTIITAMRMLDNVIDINYYAVKKARDSNLRHRPVGMGIMAFQDSLYAQRIPYASDAAV
                     AFADTSMEAVCYYAYWASTELARERGKYSSYKGSLWDKGVLPLDTLDMLASARGGYVE
                     VDRSSTLDWEALRKKIAQDGMRNSNCVAIAPTATISNIIGVDASIEPCFGNLSVKSNL
                     SGEFTVINSYLVRDLKRLGLWDDVMVMDLKHFDGSLSPIDRVPAEIKQLYATAFEVET
                     RWLVEAAARRQKWIDQAQSLNIYMAGASGKKLDETYKLAWLRGLKTTYYLRTMSATHA
                     EKSTVSAGRMNAVSSGSDAQGSTRTSSALEAAAAAAQMQMEMATNAATDIKFCGVDDP
                     TCESCQ"
     gene            complement(14092..>14408)
                     /locus_tag="PKX82_00720"
     CDS             complement(14092..>14408)
                     /locus_tag="PKX82_00720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOF50794.1"
                     /translation="CDDRAWHAGASHYRGRDNCNDDSIGIELEGLEGEPFEAEQYEAL
                     QALCPAIAQRYPIAHIAGHEHIAPGRKADPGAGMQWAFLQQTLGFPSRCFPDGVREKY
                     SF"
BASE COUNT         2861 a         3902 c         4444 g         3201 t
ORIGIN      
        1 gccagcgagg cctactgggt ggaaaacggc aaggtgcaat acccggtcaa gggtgccacg
       61 attgtgggta atggtccgga tgcgctgaca cgtgtcacca tgatcggcaa caacatgaag
      121 ctcgacagcg gcgtgggcac ctgcggcaag gaaggtcaga gcgtgccggt gggcgtgggc
      181 caaccaaccc tgcgcatcga tggtctgacc gtgggcggaa cggcctgagc ggagctgtca
      241 gcctgccgtt ttcaacctgt gctacattgc aagccatgaa gtcagctgtt ttcttcttta
      301 gctatttttg gttctcacgc gcatccggcg gtcgagagat ctgaacgtac ctacaagaaa
      361 cgtcctgaat cttccaaaaa ccgccggcac ccccggcggt tttttttatg ccttttgatt
      421 tcaactttga ctctaggagc ttccaacatg actgcaaaag cctctgcaac cggtgactgg
      481 aacggacaaa tgcatcccgc cgaccgcacc agcaaaaccg acgacaaacg catcaaggac
      541 atcaccgtgt tgccacctcc tgagcacctg atccgcttct tcccgatccg cggcacggct
      601 gtggaaaacc tgattgctga tacgcgcaag agcatccaca acatcatggc cggcaaggac
      661 gaccgcctgc tggtcatcat cggtccctgt tccatccacg accctgcagc cgcactggat
      721 tacgcacgcc ggttgaaaga gcagcgggag aagtacaagg acacgctgga aatcgtgatg
      781 cgcgtgtact tcgagaagcc ccgcaccacg gtgggctgga aagggctgat caacgacccc
      841 tacctggacg agagcttccg catcgacgaa ggcctgcgca ttgcgcggca actgctcatc
      901 gagatcaacc gcctgggcct gcccgccggc agcgaattcc tggacgtgat ttctccccaa
      961 tacctggggg atttgatctc ctggggtgcc attggcgcgc gcaccaccga gagtcaggtg
     1021 caccgcgagc tggcctcggg cctgtcagcc cccatcggct tcaagaacgg aactgacggc
     1081 aatatcaaga tcgcgaccga tgcgatccag gccgctgcgg gcggtcacca cttcctgtct
     1141 gtccacaaaa acggtcaggt ggcgattgtg caaaccaatg gcaacccgga ctgccacgtc
     1201 atcctgcgcg gtggcaaggc acccaattac gatgcagcca gcgtggcggc tgcctgtgcc
     1261 gatctggcca aggccaaact gcctgagacc ttgatggtgg attgcagcca cgccaacagc
     1321 agcaagcagc atgagaagca gctggatgtg gccaaggaca tcgcgatcca gatcagcggt
     1381 ggatcccagc gtgtgtttgg cgtgatggtg gaaagccacc ttaacgcagg agcccagaaa
     1441 ttcacgcctg gcaaggacga ctctgccaag ctggaatatg gacgcagcat caccgacgcc
     1501 tgcctggggt gggatgactc agtggcttcg ctggatgtgc tggcgcaggc tgtcaaggcg
     1561 cgacggacgc gcaaaccagc gtaacgggca ttcaggtcgg tgccggtttg ccggtgctgg
     1621 ccgcgtcaat catggcctga gcccatttca gggcttcggg catggtttca aacagcgcaa
     1681 atggtcgacc gatatcggcg tagatcttgg cgtacaaagg ggccatgagg cgaccgcctt
     1741 caatctccgg gccaatcacg aacgccgtgg caatgggcac catgttgtcc ggtttggggc
     1801 tcgccatgat ttcggcgtaa cgcgccaggc cttccggact ggtcatggcg ctttccacca
     1861 tggtgcagac actgacccag ttgccctgtg ggcggatgga cagcagatag tcccgttggg
     1921 cgatacccat caggccgacc aattccttgt taaacggccc ccggccctgg taatgcatta
     1981 acgggccatc catcgagatc tcaacaatcc cgtgcactgt aaacggtcca ttgcccgcga
     2041 tttgcgtggt actgagtttg gcggttttgg tgtctggcat atttccgggc aatcgttctg
     2101 ggcaggatgc tacgcctttt gcagcagggt aaccagtttg gcgtgaatgc caccaaatcc
     2161 cccgttgctc atgcaaagaa tgtggtcgcc gggacgtacc tgggccatca cctgctgcgt
     2221 gagttgttcg atggtgtcgg ctaccgatgc tttggggcct agcggggcaa gggcctctgt
     2281 ggcgtcccat cccaggcctc cactgtggca gaacgccagg tctgcttctt ccaggctcca
     2341 gggtagctgg gccttcatgg cgccgagctt catggtgttg gagcgtggct cgaaaacggc
     2401 cagaatgcgg gctgcacccg ccttgcggcg caagccgttg acggtggtgc ggatggctgt
     2461 gggatggtgg gcaaagtcgt cgtacacggt gacgggtgcg cccccggcgc gcaaggtgac
     2521 ctggccgcgc acttccatgc ggcgtttcac gttctcgaat gtacccaggg cttttgccgc
     2581 cagtgccggg ctcacaccca catgctcggc cgcagcgatg gcggccagcg cgttgagctg
     2641 gttgtgcacg ccacccaagg cccattgcac atgtgcgacc ggagtgccct ggtagtgcac
     2701 cgcaaagtcg tgcggctcgc cgacggcggt gaagtcactc acggcggcgc caaagctgcg
     2761 cacctcgctc cagcagcctt ggtgcaacac acgggtcagg ctttcttcca ggccattcac
     2821 cactacgcgc ccgctgccgg gcacggtgcg caccaggtga tggaactggc gctcgatagc
     2881 ggccaggttg tcaaaaatat cggcgtggtc gaattccagg ttgttcagca cggccgttcg
     2941 ggggcggtag tgaacgaact tgctgcgctt gtcgaagaac gcggtgtcgt attcgtctgc
     3001 ttcaatcacg aagagagggc ggtggggcgc cagcgccttc tgctccgtgt cccccgcccg
     3061 cgctgcgggc tcctccttta cctgcgcaga aggcgctggc gccccaccgc ccaggcgcgc
     3121 ggagatgccg aaattcatgg gtacgccacc gaccaagaag ccgggttgca gaccagcgct
     3181 ttccagtatc cacgccagca tggcggtggt ggtggttttg ccatgggtcc cggccactgc
     3241 cagtacatgg cgaccttgca gcacatgttc ggcaagccat tgcggcccgc tggtgtaggg
     3301 cgcgccggca tccagaatgg cttccatgag tggaaatttg ggtgtgccat cactcaggcg
     3361 ggtgcggctg accacgttgc cgatgacaaa catgtcgggt tgcaatgcca tctggtctgc
     3421 gccaaagcct tcgatcaact cgatccccag cgcacgcaac tggtcgctca tgggggggta
     3481 gacgccggca tcgcagccgg tcacgcggtg gcccgcttca cgggccagag cggccaaacc
     3541 gcccatgaag gtgccacaaa tgccgagaat gtgaatatgc atgcagtgat tttagccatc
     3601 agcccccgtc ctcattgcct ggccagctat caaaagtgaa gcaaaatggg tgtatggaca
     3661 caatcaagga agaaatcgct gccaccgctg cgcggatggt cgtggaggag ggactggaat
     3721 acggtgccgc caaacgccag gcgaccaagc agttgggcgt ttcggcgcgt agcgggttgc
     3781 cggacaacga ccaggtcgag gcagctgtta gggaatatat cgctctgttt tgtgcggata
     3841 cccagccggg cgaactgcgg gcgttgcgag agctggcgct ggtgtggatg gagcgcctgg
     3901 agcggttccg gccgcacctc gccggggcgg tttggcatgg cacagcgaca cgccgctccg
     3961 acatctacat ccagctgttt tgcgacgatc ccaagtctgc tgaaattggg ctgatcgaca
     4021 tgggtgtgcg gtttgaggtc accagcgtca atggcttcca gggatatccg gtcgatgccc
     4081 tcagtctgca tctgcgctgc gccccactgg gggagtatgt cgggctgcac ctgatggtgt
     4141 atgactttga cgatttgcgc ggcgccctca aacccgatgc gcagggacgg cgaccccggg
     4201 gcgatttggc ggcggtgcgc ggtttgttgc atgatgtcgc cccatgagtt ctgatcaaaa
     4261 tgaatctgcc aacggtgcgc aagcacgggg gcatcagcgc cggatgctgg tcggtgctgc
     4321 ggttgcagca acggttgccg gggcgggaat cgccatgtgg cgtaccaggt cggacgctgt
     4381 agcgccggcg cggagcgagc cagtggatgg cttctgggcc ttgcagtggg aggttccgca
     4441 aggaggtgac ttcatgcgca tggcggattt tcgtggccgc ccgctcctgc tgaacttctg
     4501 ggcgacatgg tgtcctccgt gtgtggagga attgccgctt atcaacgctt tctacaaaga
     4561 aaatctggct aacggctggc aagttcttgg attggctgtg gacaaagcat cggcagtcca
     4621 ggcatttctg aagaagatgc cactcgagtt tccagtagga atggccggta ttgctggtgc
     4681 agacttgggt cgaagtttgg gtaatttggc cggtggtttg ccattttcgg tggcgttgtc
     4741 ggcaaatggc agcgtaatcc agcggaaaat gggccgtttg acggcgtcgg atttgcaggc
     4801 ttgggctcgg ttaaaatagc cggacctgta cgccatccgt ggggcgtctg gcctcttcta
     4861 ctctatgtgg agtgaattca tcggaatatt gatccagatg aaatttgcct tcaaataatt
     4921 atgttttccg ttgttttggc gctttttgct gtaaattcgc cccttgctat acaacgtcag
     4981 ttggagattc catggattta cgcaaactta aaaccctgat cgatctggtg tctgactcga
     5041 atgtgtcaga gctggaaatt accgaagctg aaggcaaggt gcgcattgtg aagggtgggg
     5101 gtgcagtcgt gcaaagttat gcacccgctc cagtctatgc cgcccaggcc cctgctgttg
     5161 ctgcgcctgc cgctgcagcc gcaccggccc ctgtggcaga acccgttgcc gaggggcaca
     5221 cggtcaagtc tcctatggtg ggaactttct accgggcggc atctcctggc gccaaaccgt
     5281 ttgtggaggt gggtgactcg atcaaggtgg gggaaaccat ctgcatcatc gaagccatga
     5341 agatcctcaa cgagattgag gcagacaagt cgggcacggt caccaggatt ctgtgtgaca
     5401 acggccaggc ggtggaatac ggccagcctc tgttcatcat cgaataaacc agcgcaagca
     5461 gcccatgttc aagaaaatcc tggtcgccaa ccgtggcgag attgcgttgc gaatccagcg
     5521 cgcctgccgc gagttgggcg tcaaggcggt gatggtctac tccgaggctg accgtgaagc
     5581 caagtatgtg aagctggccg aagaggcggt atgtatcggt ccggccccgt ctggacaaag
     5641 ttacctcaat atgccggcca tcatctcggc agcggaagtg acagatgccg aggccatcca
     5701 tcctggctat ggtttcctga gtgaaaacgc cgatttcgcc gagcgcgtgg agaaaagcgg
     5761 attccagttc attggccctg cgccggagtc catccgcatc atgggcgaca aggtatctgc
     5821 caagcaggcg atgattcgtg caggtgttcc ctgcgtgccc ggctcagacg gtgaattgcc
     5881 ggatgacccg gtgcagattc ggcgcattgc caaggcggtt ggctatccgg tcatcatcaa
     5941 ggcggctggc ggtggcggtg gtcgtggtat gcgtgtggtg cacaccgagg cagccctggt
     6001 gaacgccgtg gcgatgacca aagcggaggc tggtgcggct ttcaataatc cggcggtgta
     6061 tatggagaag tttctccaga acccgcggca tatagagatc cagatcattg ccgacaagta
     6121 caagaacgcg gtgtatctgg gcgagcgcga ctgctccatg cagcgacgcc atcagaagat
     6181 tctggaagag tctcctgcgc ctggcatcaa tcgcaagctg atcgaacgca tcggtgagcg
     6241 ctgtgtggct gcgtgcaaga agatcggata ccgcggcgcc ggcacctttg agtttttgta
     6301 tgaggatggt gagttctatt tcattgaaat gaacacgcgg gtccaggtgg aacaccccgt
     6361 tacggaaatg gtgacgggca tcgacatcgt caagacgcaa atcatggtgg ctgccggcga
     6421 gaagctgccg ttcacccaac ggcagatcga gatacgcggg catgccatgg aatgccgtgt
     6481 caatgcggaa gatccttata agttcactcc gtccccgggg cgtgtcacca tgtggcatgc
     6541 accaggtggc cccggcgtgc gcgtggattc ccatgtgtac acgaactatt ttgtaccccc
     6601 caactacgac tcgatgattg gcaagatcat tgtccatggg gatacccgtg agcaggccat
     6661 ggcgcgcatg cgcaccgcgc tggctgagac cgtcgtggaa ggcattaaca ccaacattcc
     6721 gttgcaccgg gaattgatgg tggatgccaa gttcatggcg ggtggtacca acatccacta
     6781 ccttgagcag tggctggacc aacacaagcg gtaagcccca tgtttgaact gcgtctgatg
     6841 tgcccggagg atcgggtaga gggtgtgagt gacgcgctgg atgcgctgga cgcgttgagc
     6901 gtcagtgtcg aagatgcaga tgcacagaca gatgctgaac aggccctgtt cggagaaccg
     6961 ggcatgccgc cgcccaagga tggatggcaa cgttcgcgca tcattgcgct ctttgccagc
     7021 gaggcactcg cccgggaagc tgctgcgctg cttcatgcgc aagacttttt cgccggatgc
     7081 caggtgctgg atgtgctctc ggtacctgaa caggactggg tgcggttgac gcaatcccag
     7141 tttgcacccg tggaggtaac gcctgagttc tggattgtcc caacatggca tgagccaccg
     7201 gcgcaggcca ggcaagtcat acggcttgat ccgggtcttg cctttggaac tggtacccat
     7261 cctacgacgc ggatgtgctt gcggtgggtg gcgacacacg ctgtgaacgg ccgtgttctg
     7321 gattacggat gcggttcggg cattcttgcc attggggctg cgaaatacgg tgcccaggaa
     7381 gtcgttgcgg tggatatcga cgaggcggca gtgcagtcaa ctgagttgaa tgctgcagcc
     7441 aacgaagtcc aactgtcggc agggctgccg gaaaaggcac aaggcacgtt tgacgtggtg
     7501 ctggccaaca ttcttgccac gccgctcaaa gtgctggcgc cgcttctgtg tgcgcatgta
     7561 cgcaagggcg gaaatctggt gctggccgga attctggcgc gacaggaaga cgagttgaaa
     7621 gaggcctatg cgccctattg ccagttgacg gtcgccgaca gcgaggacgg ctggattctg
     7681 atgactgctt cagtctgaat tgcagccctg tcgggcccta caatgcgcag cagatgagcc
     7741 tgattacccg atgccccgcc tgcgccacga tgttcaaggt cgtgccggac cagctccgcg
     7801 tctctgatgg ttgggttcgt tgcggacagt gcgacgaagt gtttgacgcc aatgcacatc
     7861 tgcagtctcc tgctgaagtg gaatcggagt cagtcgcgcc ggaagattct tcgcagttgg
     7921 attgggacgg cactcttgaa gaagcagccg caccgacatc agaaattcag ctggactttc
     7981 ccgacgaagt agaccgcaat tcagcgcacg cagtgcgacc cagtgcagag gatgaatctg
     8041 cggttccgga tgtgaatcct gagggtccat tggatcattt ggatggagca gatgcgcctc
     8101 cggtcgcggg ggatcctctg gacgctattc cgggtgacga tgtcaaagac cttcaggcca
     8161 ctgcagaaga tgtgcagcca gtgcttccgt tcgtggagtc agcagacaag gatttggaag
     8221 gcaatggcgc cacgcccccg ggtttcctca gaaagtcgca cacaggctcc cgtcgaagta
     8281 gccggtccac acgggtttta ggcgtcacgt tctgtgtgct gctggtgtgt tctctgggca
     8341 tgcagattct tgtgcaggag cgagaccgta tcgtggcaac agaatcggca acacttgcgc
     8401 tactgcagcc ggtgtgcgac gtgcttggct gcagaattgc gcctttgcgg cagattgaat
     8461 cggtggcgat agaccactcg gcattcagca aggttcagtc agacgtgtac agattcagct
     8521 tcgctatcaa gagtacggcg ttggttgccg tggcagtacc atccttggag ttgacattga
     8581 cggatctgca ggatcgccct gtgatccgtc gcgtgttttc accgcgggag ttgggtgcac
     8641 aggtggaggt ggtcgcaccc gggggggagt ggtccggctc tgtgccagtt gaagtcaaaa
     8701 tgccggacgg tgcagcgagt attgccggtt accgaatgct cgctttctat ccataaaaaa
     8761 agcccgggac tgaaaagttc cgggctttgg gctccaatca acgaatcgat cagggtttgc
     8821 tggacgtagg aaacggccaa gcagcctggg gattcagtgt ggtttgagca gcaggtgcag
     8881 caggggctgc tttggcagcg ggtgctttcg cggctttctt cgcagccggc tttttggctg
     8941 cctttttagc tgctggcttc ttcgcggctg gctttttggc tactgcagct tttttggctg
     9001 gagccttttt agcagcggct ttctttgccg gggcagcctt tttagcggct acttttttcg
     9061 ctggtgcagc tttctttgca gctggctttt tggcagcagc agcttttttg gctggagcgg
     9121 ctttcttagc gggtgctgcc ttcttggcgg gggcagcttt cttagctgct acttttttcg
     9181 ctggtgcagc ttttttcgct ggtgcagctt ttttcgctgg tgcagctttc ttagcgggag
     9241 cggccttttt tgcggccggt ttttttgcag ttgccatcat tttctccttg atcaatttac
     9301 aaagagcact tcttgcgtgt tggatacaag aagcgattca tgtcgttggg tttggtccca
     9361 gcgaaatgaa ctcggtgaca cgctctccaa tgaccatcgc tgcggtcttg tggagtggcg
     9421 tgaaattttt aaaaacatgt tgatgcgtta atcccaagac agcgcgccac ccgactggta
     9481 ctcaatgacg cgtgtctcga agaagttacg ttccttcttc aagtcgatca tttcgctcat
     9541 ccaggggaaa ggattctctt cgttggggaa caaggcttcc aagccaattt gcgtggcacg
     9601 gcgatttgcg atgtagcgca agtagccttt gaacatggac gcattcattc ccagtacgcc
     9661 gcggggcata gtgtcttcag cgtagcggta ctccaactcc acggcctttt caaacagggc
     9721 cttgatctcg gccttgaact ctgcggtcca gaggttgggg ttctcggcct tgagctgatt
     9781 gatcaagtcg atgccgaaat tgcagtgcat ggattcgtca cgcaggatgt actggtactg
     9841 ctcggcagcg cccgtcatct tgttttgacg gcccaatgcc aaaatctgtg tgaagccaac
     9901 gtagaagaac aaaccttcca tcaggcacgc aaacacaatc agcgacttga gcaacgtctg
     9961 gtcggtttcc tgggtgcctg tgtggaagtt ggggttcatg atcgcctcaa tgaaggggat
    10021 caggaattca tccttgtcac ggatggactg gacttcgttg taggcgttga aaatctcgcc
    10081 ttcgtcgagc cccagcgatt ccacgatgta ctggtacgca tgcgtgtgaa tggcttcctc
    10141 aaatgcctgg cgcagcagaa actggcggca ttcaggtgct gtgatgtggc ggtaggttcc
    10201 caaaacgatg ttgttggcag ccaatgagtc cgccgtgaca aagaatccca gattgcgctt
    10261 cacgatacga cgctcatctt cggtcagacc gttggggtct ttccaaagcg cgatgtcgcg
    10321 ggtcatattg acttcctgcg gcatccagtg gttggcgcaa gtagcgaggt acttctccca
    10381 cgcccacttg tacttgaagg gcaccaactg gttgacgtcg gtctggccat tgatgatgcg
    10441 tttgtcagca gcacgcactc gacgtggcgc tacggtaggc gaggcattga tgctggaaga
    10501 agatggaact agcgcgccat cgtccaaggt gcgcaagttc aaagaggaag aggactcctg
    10561 caccagaggt gcattgacag cagccgtacg tgcaagattt gtcggcgtag agggcttaac
    10621 ttcgtcgtcc caggtcagca tagatgtttc caatcagcga attatgagag cagcgaaatg
    10681 aaatgaaaag caatcatgtc attcgctgcg aatttgtgtt gttcaatagc atcgcattga
    10741 agctgcgcga tcacgcgact tcattggcac gactcgcacg ttgggtcatc cacaccgcag
    10801 aatttgatgt cggtggctgc gttggtcgcc atttccatct gcatttgcgc ggcagctgct
    10861 gctgcttcca gtgcgctgga ggtgcgtgtc gagccctgcg catcactgcc ggatgacact
    10921 gcattcatac ggcctgccga tacggtagat ttttctgcgt gcgttgccga catggtgcgc
    10981 aagtagtaag tggttttgag gccacgcaac caagcgagct tgtaggtttc gtcgagcttc
    11041 ttgccagagg cgccagccat gtagatgttc aaggactgtg cctggtcgat ccatttctgt
    11101 cgacgtgcgg cggcctccac caaccagcgg gtttccactt cgaatgctgt tgcatagagc
    11161 tgcttgatct cggcgggtac gcggtcaatg gggctgagag agccgtcgaa gtgtttcaga
    11221 tccatcacca tcacatcgtc ccacaggccc agacgcttga ggtcacggac caggtagctg
    11281 ttgatcacgg tgaactcgcc cgacaggttg gacttgacgg acaggttgcc gaagcagggt
    11341 tcgatggacg catccacgcc aatgatgttg gaaatggttg ctgtaggcgc aatggccacg
    11401 caattggagt tgcgcatgcc atcctgtgca attttcttgc gcagggcttc ccagtccagc
    11461 gtggaagagc ggtccacctc aacgtagcca ccgcgtgcag aagccaacat gtcgagcgtg
    11521 tcgagcggta gcacgccctt gtcccacaga gaccccttgt agctggagta tttgccgcgt
    11581 tcacgggcca gctcagtgga ggcccagtag gcgtagtagc acacggcttc catggaggtg
    11641 tccgcaaagg ctacagccgc atcggacgca tagggaatac gttgggcgta caggctgtcc
    11701 tggaaggcca tgatgcccat gcccaccggg cggtggcgca agttggagtc gcgcgccttc
    11761 ttgacggcgt agtagttgat gtcgattacg ttgtccagca tgcgcatggc cgtaatgatg
    11821 gtgcgcttga gtttgtcgtg gtccagctcc ttgccgttgg caccgtcttt caggtgctga
    11881 agcagattga ccgagcccag gttgcacacg gcagtttccg tgtcgctggt gttgagggta
    11941 atttcggtgc agagattgga agagtgcact acccctgcgt gttgctgggg gctgcgtaca
    12001 ttgcaggcgt ccttgaatgt gatccaggga tgaccggttt cgaacagcat ggtcagcatc
    12061 ttgcgccaca ggtcggcggc ctggatggtt cgtgcgggct tgatatcacc acgtgcggct
    12121 ttctcttcgt aggccacata ggccttttcg aagtcggcgc caaacaggtc atgcaggtcg
    12181 ggcacgttgg atggggagaa gagcgtccag gttccttttt ccatcacgcg gcgcatgaac
    12241 aggtcgggaa tccagttggc cgtgttcatg tcgtgcgtgc ggcggcggtc gtcgccggtg
    12301 ttcttgcgca actccaggaa ctcttcaata tccaggtgcc atgtttccag ataagtgcag
    12361 acggcacctt tgcgcttgcc gccctggttc actgccaccg cggtgtcatt aacaaccttc
    12421 aggaagggca ccacaccctg cgattcgcca ttggtcccct tgatgtggga gcccagggca
    12481 cgtacgcgtg tccagtcgtt gcccaaacca ccggcaaact tggacagcaa tgcgttctcc
    12541 ttgatggact catatatgcc atccagatcg tcaggcactg tggtcaagta gcagctggag
    12601 agctgcgagc gcagcgtgcc gctattgaac agggtaggcg tactggacat gaagtcgaag
    12661 ctggagagta cctcgtagaa ctcgatggcg cgggcttcgc ggtcaatctc attgagtgac
    12721 aggcccatcg ccacacgcat gaagaaagcc tgtggcaatt caatgcgtgt cttgcccacg
    12781 tgcaggaagt agcggtcata cagggtttgc agacccaggt aatcaaaatt gagatcgcgg
    12841 tctgccttca gggcagcgcc gaggcgcttg aggtcaaact gcagcaactt ttcgtccagc
    12901 agttcagcgt ccacgccttt cttgatgaaa ccggggaaat agtcgcaata ggcttggccc
    12961 atgtcggctt gtgccacgtc acgtcccaga acctcgcggg agatcgtgtg gaaaagcaga
    13021 cgggcggtgg cataggtgta gtccgggtct ttttcaatca gcgtgcgtgc agccaggatg
    13081 gatgccttgt agacctcatc catgggcaca ccgtcgtaca ggttgcgcat ggtttctgcc
    13141 acgatggggt ccgccttgac atctgcaccg agacccgcgc atgcgacttc tatgcgtgtc
    13201 ttgagttgct ccagatccag agcgatacgc tggccacctt ccagaacgtg cagggtgggg
    13261 gcggccggag cggcttgtgc tacctgcttg gcgcgttctt gtgtgcgccg ctcgcggtac
    13321 agaacataag cgcgagcgat ttcgtggtga ccgccgcgca tcaggccgag ttcgacctga
    13381 tcctgaacgt cttcgatatg aaatgtgccg ccaccggggc gtgagcgcat caacgcacgg
    13441 ataacctgtt gcgtcagtcc gtccacggtt tcgcgcacgc tggcggaagc ggctccctgc
    13501 gtgccatgca ctgccagaaa ggccttcatc atggccacgg caattttgtt gggctcgaac
    13561 ggcactactg cgccattacg acgaatgatc tggtagtgcg ccagcggact agtggattcg
    13621 cccgcgaagg ccggcacatt ggtcgcggct atggagcttg tcgtcggtgt gggggctgca
    13681 gatgagacag attgcatgat ttcctcgtct taacggtgat ctttttttgg ggggtgatgg
    13741 cgcgtttgtc ggtttttgca ctggatgcct ggcgcctcaa agcgaacaga acacactata
    13801 tctggggtgt cgggtggtga caagccacta ccggtagtgt atcgaaagaa tgcggctact
    13861 tttgtctgtg gatgaaagct ggccagaaat atgcacgaaa ttcgctggaa aagacgggtt
    13921 ccaggaatct cgcgcacagc catattccac gccgttttta tctgtatttc cagtatcgac
    13981 gcggctctct gaatgttttt cagtgaaaag ccgcatcgtt attggactgg cgcgaggtgg
    14041 gctcatgtcg ggcgcatcga atggtgcaga gccacacccc gaattccgag cctaaaaaga
    14101 atatttttcc cgaacaccat cgggaaaaca ccgagaagga aatcccagcg tctgttgcaa
    14161 aaacgcccac tgcattcctg cgcccggatc tgcttttctg cctggtgcaa tatgttcatg
    14221 cccagcgatg tgcgctatgg ggtagcgctg ggcgattgcc gggcatagtg cttgtagtgc
    14281 ttcgtattgt tcagcctcga agggctcacc ttccagccct tcgagttcaa tgccaatgga
    14341 gtcgtcgttg cagttgtctc gaccacggta gtgagaagcc cctgcatgcc aggcgcggtc
    14401 atcacaac
//
LOCUS       DAOSJM010000009        14274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4346_length_14274_cov_4.119769, whole genome shotgun sequence.
ACCESSION   DAOSJM010000009 DAOSJM010000000
VERSION     DAOSJM010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14274
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4346_length_14274_cov_4.119769"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PKX82_00725"
     CDS             complement(<1..387)
                     /locus_tag="PKX82_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOF50795.1"
                     /translation="MKFHGTKNYVATQDLMLSVNAAITLQRPLLVKGEPGTGKTMLAE
                     EVAQALDMPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDARVSDIRNYIKRGKLWE
                     AFSAPARPVLLIDEIDKADIEFPNDLL"
     gene            519..1178
                     /locus_tag="PKX82_00730"
     CDS             519..1178
                     /locus_tag="PKX82_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOF50796.1"
                     /translation="MNKFLTSFFSVVVACVTASSVHAQEIKGDAFAGEKINAMCVGCH
                     GIVGYHTGFPEVHRVPMISGQSAKYIVAALNAYKKGERKHASMRGVAANLSDKDIADL
                     AAYYSDNGPRRPEPEKAVPGSAKAQELLTRGGCTSCHGDSFSKPVDPSYPKIAGQHAD
                     YLYVALKAYKTEGVPQIGRANAIMGGVAKQFSNAELKELANYLGSLPGELQTVQPGRF
                     R"
     gene            complement(1240..1674)
                     /locus_tag="PKX82_00735"
     CDS             complement(1240..1674)
                     /locus_tag="PKX82_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HOF50797.1"
                     /translation="MFSPSQADVRRFFCSVYAKARAGSALEAIETIASQWMDEHPEYH
                     ADFADVDTALATMYESEEGRTNPFLHLSMHLSISEQCSIDQPRGIRQAVELLTHKRNS
                     LHDAHHDAMECLGTMLWESQRAGRPPDGNAYIACVQRHATRD"
     gene            1733..2488
                     /locus_tag="PKX82_00740"
     CDS             1733..2488
                     /locus_tag="PKX82_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOF50798.1"
                     /translation="MTFPLITDPFFYAVTIPAVLLLGISKSGFGAGFGSLAVPMMALA
                     VSVPEAAAILMPVLFVMDVLGMAAFRKDFDLKLLKFLIPFGMLGIAIGALLFKVLNAH
                     TVAALVGGFTLLFLAQRLLFPPTLHSPPPPKWLGAILTTTSGFTSFIAHAGGPPINAY
                     VIPLKLSPIQFTATMAFFFFVVNLCKWVPYGMLGLLDLRNMATSLVLLPLAPLGVWVG
                     VRMARRISQVLFYRFLYAGMFLTGVKLLWDGLR"
     gene            complement(2501..4306)
                     /gene="kefC"
                     /locus_tag="PKX82_00745"
     CDS             complement(2501..4306)
                     /gene="kefC"
                     /locus_tag="PKX82_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HOF50799.1"
                     /translation="MEHSPAWLINSFIYLSAAVIAVPLSKAIGLGSIIGYLTAGIAIG
                     PWGLGLVTNVEDILHFAEFGVVLMLFLVGLELEPKRLWSLRRPIFGWGSAQVLGCALL
                     LAAVAMLLGVDWRTSLVAGLGLALSSTAIALQVMGERNLMRTGSGQSAFSILLFQDVA
                     AIPILALLPLLGAAYASNEPAAPVNAAQDALKIVAVIASIVLGGRLLLRPLFRWIARS
                     NTPEIFTAAALLLVVGIAALMQLVGLSMALGAFLAGVLLAESEYRRELETDIEPFKGL
                     LLGLFFIAVGMSIDFGVLWQSPGLMAAIVVGFLVVKGLVIWGLARMMDQPFQERPVFT
                     LLLAQGGEFAFVVFQAAAGAHVFSAQTASLLIGAVAVSMLVSPLILVAVDRWLLPRYA
                     HCGVPQLAEISEQQEAPIVVAGFGRYGQIVARTLLAQGIPCTVLDHDAEMIEAARNFG
                     YRVFYGDATRLDLLRTAGAANARILVLAVDDVDQSLEIVDLVQAHFPHLRIVARAHDV
                     THWNKLRDRGVMKVEREMFESSLLSARSVLEMLGRSPEEAQQITTRFRQHNLELFEKL
                     HPHYRDQAKLISVIKEGRAQLEEQMAQERAEQANR"
     gene            4351..4968
                     /locus_tag="PKX82_00750"
     CDS             4351..4968
                     /locus_tag="PKX82_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOF50800.1"
                     /translation="MPVVVVANPKGGVGKSTISTHVAGYFASQGHAVMLGDADRQQSS
                     RLWLGLRPTAARPIQAWDIGPDLIAKPPKGTTHVVLDTPAGLHGWRFRDVMKMADKII
                     VPLQPSVFDIFATRAFLDELASNRDAMKADIGIVGMRVDARTIAADKLHEFVDSLGLP
                     VLGYLRDTQNYIHLAARGLTVFDVAPGRVERDLAQWQPICQWLED"
     gene            5105..5560
                     /locus_tag="PKX82_00755"
     CDS             5105..5560
                     /locus_tag="PKX82_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOF50801.1"
                     /translation="MKTFQTLAELAALVGQEVAFSDWVLVTQERINQFADATGDHQWI
                     HVDVERAKSGPFGAPIAHGFLTLSLLPTFFDNAMKVEQVRMGVNYGLNKVRFVAPVPV
                     GSQLRARIKLLKAEGIDNNGLQTTWEVTVEREGAPKPVCVAESLARMYP"
     gene            complement(5636..6097)
                     /gene="trmL"
                     /locus_tag="PKX82_00760"
     CDS             complement(5636..6097)
                     /gene="trmL"
                     /locus_tag="PKX82_00760"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HOF50802.1"
                     /translation="MFHIVLVEPEIPPNTGNVIRLAANTGCTLHLVEPLGFSMDDKHM
                     RRAGLDYHEYAQLKRHASWQTFLEHEQPAPDRLFTLTTRGTRSPYEVAFQAGDWLVFG
                     SETKGISDLVRASFGPGQSLRLPMREGQRSLNLSNAVAVTVFEAWRQNDFA"
     gene            complement(6105..6800)
                     /locus_tag="PKX82_00765"
     CDS             complement(6105..6800)
                     /locus_tag="PKX82_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOF50803.1"
                     /translation="MFRPLIEGISQRLPSQCAICHTWPAQPVCDACVAAFAQPVPRCI
                     TCALPVPAGVQRCGDCLLHPPPVDQCLAAVAYAYPWSGLVVDFKFHQHTGWARTLALL
                     MRSAPWVEPALDQADLLIPMPLSSQRLQERGFNQTLELARTLEPAKVAQGLLLRLHDT
                     PPQSALPRTERMRSVQGVYAVEPLQAGALKDRRVVLLDDVMTTGASLFAAAQVLREAG
                     AAHITGLVFARTE"
     gene            6850..7758
                     /locus_tag="PKX82_00770"
     CDS             6850..7758
                     /locus_tag="PKX82_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF50804.1"
                     /translation="MRTERPPTIDPVAAARWEQRVPAESPWLHEEVARRMEDRLQWIV
                     SQPRRWAHWAPLQGGLQAQALLAKRYPKSECFVVEAPVERVQAARKLIAKSRWNPSQW
                     GSAKVRFEVPAEPVQMVWANMALHMAPDPMDLIARWNQVLDVDGFLMFSCLGPDTLRE
                     LHGLYRAMGWAPPTHAFTDMHDWGDMLVQAGFAEPVMDMERISLSYSSPQSLLEELRG
                     LGRNLHHGRFAGLRGRTWHQSLQDAIGRELADPAEGGRLKLTFEVIYGHAFKPKARMP
                     VSPQSSITLQQMRGALGLNHEGKGTK"
     gene            7755..7988
                     /locus_tag="PKX82_00775"
     CDS             7755..7988
                     /locus_tag="PKX82_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HOF50805.1"
                     /translation="MTGFQAELRWGLMVVCLLIFGLAFAVMVVSAWVQHRRGAKETTN
                     FHGSVAVEVCWALAPLVIVMQLVWPTVRSVLAP"
     gene            8120..8917
                     /gene="coxB"
                     /locus_tag="PKX82_00780"
     CDS             8120..8917
                     /gene="coxB"
                     /locus_tag="PKX82_00780"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOF50806.1"
                     /translation="MQGLKKTVLGALVALGPAAVWAGYEFSFPKPVTPIAHETLHVHN
                     LFMVIALTLFFGVLALMLYSFWKHRKSVGHKAADFTAPKSRKQWLWTLFPFAALLVLD
                     YGVFGIPAYHAVLMYEDTKTDAELVVKVTGSQWLWEYEFPAEGVKFYSRLATPRAQIL
                     NEQPKGEHYLLEVDNPLVLPVGKKIRFITTSNDVIHSWWVPAFGVKRDAVPGFLREFW
                     AKIDTVGTYRGQCSELCGKEHAYMPIVVNVVPQAEFDSWLKSKKTPL"
     gene            8968..10575
                     /locus_tag="PKX82_00785"
     CDS             8968..10575
                     /locus_tag="PKX82_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 1"
                     /protein_id="HOF50807.1"
                     /translation="MAHDLAHDLPHADGHDAHHPYGGAMRWIQTTNHKDIGLMYLIFG
                     ITMLLMGGAMILGVRAELFRPGLQLMQPEFFNQLITLHGLIMVFGFAMPVATGLANYM
                     LPMVLGAPDMALPRLYNWGFWILPPAAVMLVLPFFLGLVGIGPGAVDTGWTMYAPLSV
                     QSGWGMDFAIFAVHMLGVSSILGSINVIVTILNMRAPGMTLFKMPLFAHGFLVTAILL
                     ITIMPVFAGGVTMVLMDRHFDTHFFNAAGGGDPVLWQHIFWFMGHPEVYVLLLPITGM
                     VPHVLTAFSRKQTYGYRAQVYSMWAIGVLAMVVWAHHMFTSGMSTGGQLYFMYSTMLI
                     SLPLAVMFFCWIATLWRGSLSFETPMLFAVGFIILFGWGGLTGLILADAAADPQYHNA
                     YFLVAHFHYALYPTTVMGAMAAIYYWLPKWTGHMLNERMGRIHFWVVMIGFNLTFIPQ
                     FMVGLAGMPRRIPDYPLMFTEWNMLSTVGAFILGASHLIFIYNVWSNIRGGDKAEAKA
                     WEGAEGLEWTVPSPAPYHTFEKQPTIE"
     gene            10596..10742
                     /locus_tag="PKX82_00790"
     CDS             10596..10742
                     /locus_tag="PKX82_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50808.1"
                     /translation="MSAPDMQPNPTDADVWAQRRRNNVRLGWALGAAAVAIFLIAVLK
                     YRPL"
     gene            10739..11293
                     /locus_tag="PKX82_00795"
     CDS             10739..11293
                     /locus_tag="PKX82_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOF50809.1"
                     /translation="MSAEASTALQQDNRRLGFRLLLVAIAFGGLGFAMVPLYDVICRI
                     TGLNGRTNATAIAADKNTQVDMTRMVNVEFLSHTMPGVGLEFKPEQFTMRVHPGEVMH
                     TTYTVTNTSKEVFVGQAVPSVTPAVAAPYLQKIECFCFDQQTFQPGEVRTMPVVFVIK
                     PDMSRELGTVTLSYTFFEAPKSRI"
     gene            11328..12191
                     /locus_tag="PKX82_00800"
     CDS             11328..12191
                     /locus_tag="PKX82_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOF50810.1"
                     /translation="MTPSPTPQSQGHYFVPAVSNYSTFLSAGIFMLALGFIFKLNGVP
                     AGTWSMLFGAALILYVIFGWFGEVISENVSGVYTLWEDRSYRIGMVWFIFSEVMFFAC
                     FFGVLYYIRRIALPELGGYEQAYTPYAGFTGAWPSSGPLGETFTPMKAWGIPALNTML
                     LLTSGATLTWAHWGLIKNKRSQLVWGLALTVILGAVFMGFQAYEYAHAYSELGLTLGA
                     GVYGATFFILTGFHGMHVTLGTIMLIVMWARSLKGHFSEHNHFAFEAVAWYWHFVDVV
                     WLIVFVFIYWI"
     gene            complement(12218..12421)
                     /locus_tag="PKX82_00805"
     CDS             complement(12218..12421)
                     /locus_tag="PKX82_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HOF50811.1"
                     /translation="MTKIFILAILGLILASLFSALSLLFRKEGDKNRMVQALTVRVAL
                     SIGLFLMLLAGFYFGIIPQRGLK"
     gene            12475..13194
                     /locus_tag="PKX82_00810"
     CDS             12475..13194
                     /locus_tag="PKX82_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HOF50812.1"
                     /translation="MQKLRFEPRVIPTVATVCLLALFIHLGNWQAGKAERRAAEMAQH
                     SERARLGPYAIGAVLVDPGVLQDAPVTVRGVYEPEAQFFVDNRQEDGKPGVHVVTPLK
                     IEGSETRILVNRGWVGWTQGRQVLPKVATIIGVVQVSGVATVPVHKGFFLMPERAESL
                     PRLWSRLDLSRFAGEHSYPVQPVVVLQAPEDAKDGLVRRWQPPEDRVGMHQSYSYQWY
                     GMALALLVFYGVASVRTRKSS"
     gene            13191..13865
                     /locus_tag="PKX82_00815"
     CDS             13191..13865
                     /locus_tag="PKX82_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50813.1"
                     /translation="MTKLHLDEPLGLAVHSLPSPQDTAAEPSSVRGRWKLLAIMVLCS
                     LPVVASYLAYFVVRPQGKAGYGELISPVRPIAQQTGLALDGKSQALASLKGQWLLVSV
                     GPGSCAQDCQQRLFLQRQLRETLGKGKERVDWVWLVSDVAPVDTSMRKPLGDAVVLRV
                     DPSVVQDWLGVDAASAVNYLFVVDPMGNTMMRLPSQFDGAGAAKARRDLERLLRASVS
                     WDTPGR"
     gene            13871..>14274
                     /locus_tag="PKX82_00820"
     CDS             13871..>14274
                     /locus_tag="PKX82_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOF50814.1"
                     /translation="MASNPELYNLSPLGLLLLVGGALALLPLAWVWLRNRRASPAVRW
                     QALALLTLFLTFDLVLFGAFTRLTDSGLGCPDWPGCYGSASPLGAHADIAAAQTALPT
                     GPVTHGKAWVEMVHRYLATSVGALIVVLTLIA"
BASE COUNT         2612 a         4204 c         4464 g         2994 t
ORIGIN      
        1 cagcaggtcg ttcgggaact cgatgtcggc cttgtcgatc tcgtcgatca gcagcaccgg
       61 gcgcgccggg gccgagaagg cctcccacag cttgccgcgc ttgatgtagt tgcggatgtc
      121 agagacgcgg gcgtcgccga gctgcgaatc gcgcaggcgc gagacggcgt catattcgta
      181 gaggccctgc tgcgccttgg tggtggattt gatgtgccac tgcagcaggg gcatgtccag
      241 cgcctgggcc acttcttcgg ccagcatggt cttgccggtg cccggttcgc ccttgaccag
      301 cagggggcgc tgcagggtga tggccgcgtt gacggacaac atcagatctt gtgtggcaac
      361 gtaattcttg gttccgtgaa atttcatggg gaagacttca aaaggagggg attacccgga
      421 ctcgaataaa aaagcttgtt caatataatg tccgcagatc aaagacaagc caagcttgcc
      481 ttgatcgatc tcaaacaccg gttgattgtc ctctcaaaat gaataaattc ctgacatcgt
      541 tcttctccgt ggttgtcgca tgcgtgaccg cttcttccgt tcatgcccag gagatcaaag
      601 gcgatgcctt tgcgggtgaa aaaatcaatg ccatgtgcgt cggttgccat ggcattgtgg
      661 gttaccacac cggttttccg gaagtgcacc gcgtgcccat gatttccgga caaagcgcca
      721 agtacattgt ggcggcgctg aacgcctaca aaaagggtga gcgcaaacac gccagcatgc
      781 gcggtgtggc cgcaaacctg agcgacaagg acatcgccga tctggctgcc tactattcgg
      841 acaacggccc acgccgtccc gagcctgaaa aggctgttcc cggcagtgcc aaggcacagg
      901 agttgttgac ccgcggtggt tgcacctcct gccacggtga cagcttcagc aagccggtgg
      961 atccttccta ccccaagatt gcgggtcagc atgccgacta cctgtatgtg gccctcaagg
     1021 cctacaagac cgaaggtgtg ccccagatcg gtcgtgccaa tgccatcatg ggcggtgttg
     1081 ccaagcagtt ttccaatgcg gaactcaagg aattggccaa ctacctgggc agtctgcccg
     1141 gcgaactgca gaccgtgcag ccaggccgtt tccgctaagc cctgcatccg ccagcaaaaa
     1201 gccaccgcat gcggtggctt tttttatgcg tgctgaaggc taatcccgcg tggcatggcg
     1261 ctgtacgcag gcaatgtagg cgttcccgtc gggcgggcgc ccggcgcgct ggctttccca
     1321 cagcatggta cccaggcact ccatggcgtc atggtgcgca tcgtgcagcg aattgcgttt
     1381 gtgggtaagc agttccaccg cctggcggat gccgcgcggc tggtcaatgc tgcactgctc
     1441 gctgatcgac aggtgcatgg acaggtgcag gaaggggttg gtgcgtcctt cttcggattc
     1501 gtacatggtg gccagcgccg tgtcgacatc tgcaaaatcc gcgtggtact cgggatgttc
     1561 atccatccat tggcttgcta tggtttctat agcttccagc gcagagcccg cgcgggcttt
     1621 ggcataaaca gagcaaaaaa agcggcggac atcggcttgg gaggggctga acatgcggca
     1681 aggttaacat gctcgcctgt tcctgaagtg gcggccgcca ggtcgttcag ctgtgacttt
     1741 tcccctgatt acagatccct ttttctacgc agtcaccatc cctgcagttc tgttgctggg
     1801 catcagcaag agcgggttcg gggccggctt tggctcgctg gcggtgccga tgatggcgct
     1861 ggcagtctcg gtgccggagg cagcggccat cctgatgccg gtgctgtttg tgatggacgt
     1921 gctgggcatg gcggcatttc gcaaggactt tgacctcaag ctgctcaaat tcctgattcc
     1981 ctttggcatg ctggggatcg ctataggggc gctgctgttc aaggtgctca atgcgcatac
     2041 cgtggcggcc ctggtgggcg gctttacgct gctgtttctg gcgcaaaggc tgttgtttcc
     2101 acccacgctg cacagcccgc caccgcccaa atggctgggt gcaattttga cgaccacttc
     2161 aggattcacc agcttcattg cccatgcggg cggtccgccc atcaatgcct atgtgatccc
     2221 gctcaagctc tcccccatcc aattcaccgc cacgatggcg ttctttttct ttgtggtcaa
     2281 tctgtgcaaa tgggttccct acggcatgct gggcttgctg gacctgcgca atatggcgac
     2341 gtctctggtg ctgctgccac tggcaccgct gggtgtgtgg gttggcgtgc gcatggcccg
     2401 gcgcatcagc caggtgctgt tctaccggtt tctgtacgcc ggaatgttcc tcacaggtgt
     2461 caaattgctg tgggacggtt tgcgctgaac cggtgacgat tcaccggttg gcttgttccg
     2521 cacgctcctg tgccatctgc tcttccagtt gcgcgcgccc ttccttgatg actgagatca
     2581 gcttggcctg gtcgcggtag tgggggtgca gcttttcgaa cagctccagg ttgtgctggc
     2641 gaaagcgcgt ggtgatctgc tgcgcttctt ccggtgaacg ccccagcatc tccagcacac
     2701 tgcgcgcgct gagcaggctg gactcgaaca tctcgcgctc caccttcatg actccgcggt
     2761 cgcgcagttt gttccagtgg gtgacatcgt gggcgcgggc cacgatccga agatggggaa
     2821 agtgcgcctg caccagatcg acaatttcca gcgactggtc cacatcgtcc accgccagca
     2881 ccagaatgcg ggcgttcgca gcgccagcag tgcgcagcag gtccagccgg gtggcatcgc
     2941 cgtaaaacac ccggtagcca aagttgcggg cggcttcgat catttcggcg tcatggtcca
     3001 gcacggtgca gggtatgccc tgggcgagca gggtgcgggc cacgatctgg ccatagcgac
     3061 caaacccggc aacgacgatg ggggcttcct gctgctctga aatttctgcc agttgcggca
     3121 cgccgcagtg ggcatagcgc ggcagcagcc agcggtcaac cgccaccaga atcagcggac
     3181 tcaccagcat ggacaccgcg acggcaccga tcagcaacga ggccgtctgc gcagaaaaaa
     3241 catgcgcccc ggcggcggct tggaacacga cgaaggcaaa ttcgccgccc tgtgccagca
     3301 gcagggtgaa caccggacgc tcctggaatg gctggtccat catgcgggcc agaccccaga
     3361 tgacgagacc cttgaccacc aggaagccca ccacaatcgc ggccatcaac cccggcgact
     3421 gccacagcac accaaagtcg atactcatgc ccaccgcaat gaagaacagc cccagcagca
     3481 gccccttgaa gggttcgatg tcggtttcca gttcgcgccg gtattcactt tcggccagca
     3541 gcacgccggc aagaaaggcg cccagcgcca tcgacaggcc gaccaactgc atcagcgctg
     3601 caatccccac caccagcaac agggctgcgg cggtaaagat ttcgggcgtg ttggagcggg
     3661 caatccagcg gaagaggggg cgcagcagca agcgtccacc tagcacgatg ctggcaatga
     3721 ctgctactat tttcagagcg tcttgcgcag cgttgacggg ggctgcaggc tcatttgatg
     3781 cataggctgc gcccagcaga ggcagcagcg cgaggatggg gatggccgcc acgtcctgaa
     3841 atagcaggat agaaaaggcc gattgtccgc tgccagtgcg catcagattg cgttcgccca
     3901 tcacctgcag tgcaatggcg gtgctagaga gcgccagccc caggcctgcg accaggctgg
     3961 tgcgccagtc cacgcccagg agcatggcca ccgctgcgag gagtagcgca caccccagta
     4021 cctgagcgct gccccagcca aaaatcggcc ggcgcaggct ccacagccgt ttgggttcga
     4081 gttccaggcc gaccagaaac agcatcagca ccacgccgaa ctcggcgaag tgcaggatgt
     4141 cttcgacgtt ggtcaccaga cccaggcccc agggtccgat ggcaatgccc gcggtcaggt
     4201 agccgatgat ggagcccaac ccgatggctt tgctcagggg gaccgcgatg actgcagcgc
     4261 tcaggtagat gaagctgttg atcagccagg cgggggagtg ttccatgccg ctaacatagc
     4321 gcaagttcgc attttttgac aggagccgcc atgccagtcg tcgtcgttgc caaccccaaa
     4381 ggtggcgtgg gcaagtccac gatctccacc cacgtggccg gatattttgc gtcgcagggc
     4441 catgcggtca tgctgggcga tgcggaccga caacaatcct cccgactctg gctgggcttg
     4501 cgaccgacag ctgcccggcc catccaggca tgggatattg gcccggacct gatcgccaag
     4561 ccgcccaagg gaactaccca tgtggtactg gatacgcccg ccggactgca tggctggcgc
     4621 tttcgcgacg tgatgaagat ggccgacaag atcatcgtgc cgctgcagcc cagcgtgttt
     4681 gacatctttg ccacgcgtgc atttctcgat gagctggcgt ccaaccgcga cgcgatgaag
     4741 gcggacatcg gcatcgtggg catgcgggtg gatgcgcgca ccatcgcggc ggacaagctg
     4801 cacgaatttg tggattcact ggggctgccg gtgctgggct acttgcgtga tacccagaac
     4861 tacatccatc tggcggcgcg cggcctgacg gtgtttgatg tcgcacccgg tcgggtggag
     4921 cgggaccttg cgcagtggca gcccatttgc cagtggctgg aagattagcc cccacgctcc
     4981 accgctgcgc gggtcgctgc cccccgaggg ggcgaattcg ccttggggcg gcccggcggc
     5041 gaattgcctc ttggctgtca cgcgatcgac agtgctcgcc gctgactctg gctacagtgg
     5101 ccccatgaaa acctttcaaa cccttgcaga actcgccgcg ctggtgggcc aggaagtcgc
     5161 gttcagcgac tgggtcctgg tcacccagga gcgtatcaac cagtttgccg atgccaccgg
     5221 cgaccaccag tggatccacg tcgatgtgga gcgggccaag tctggtccct ttggtgcgcc
     5281 gattgcgcac gggtttctga ccctgtcgct attgcccacg ttttttgaca acgccatgaa
     5341 ggtggagcag gtgcgcatgg gcgtgaacta tggcctgaac aaagtgcgtt ttgtggcgcc
     5401 ggtgcccgtg ggcagccagc tgcgcgcgcg catcaagctg ctcaaggccg aaggcatcga
     5461 caacaacggc ctgcaaacca cctgggaagt gaccgtggag cgtgaaggcg cacccaagcc
     5521 ggtgtgcgtg gccgagtcac tggcccgcat gtacccctaa gcgtcgttgc cccccaaggg
     5581 ggctaatttg gtttcgccgc gcttcgcgaa cacttggggc ggcccggcgt gaaattcagg
     5641 cgaagtcgtt ttgccgccac gcctcaaaga cggttacggc gacggcgttg gacaggttga
     5701 ggctgcgctg accctcgcgc atgggcagac gcaggctctg gccgggacca aagctggcgc
     5761 gcaccaagtc tgaaatgcct ttggtctcgg agccaaacac cagccagtcg ccagcctgga
     5821 atgcaacctc gtaagggctg cgcgtgcccc gcgtagtcag cgtgaacagg cggtccggtg
     5881 cgggctgctc gtgttcgagg aaggtctgcc agctggcgtg gcgtttgagc tgtgcgtact
     5941 cgtggtagtc cagcccggca cggcgcatgt gcttgtcgtc catcgaaaat cccagtggct
     6001 ccaccaggtg cagcgtgcac ccggtattgg ccgccaggcg gatgacgttg ccggtgttgg
     6061 gcgggatctc gggttcaact aaaacgatat gaaacatcgt tgaattattc cgtacgtgca
     6121 aataccagcc cggtgatatg cgccgccccg gcctcccgta gcacctgcgc cgccgcaaaa
     6181 agcgaagcgc cggtggtcat cacatcgtct agcagcacca cgcgccgatc tttcagggca
     6241 cccgcctgca gtggctccac cgcgtagacc ccctgcacgc tgcgcatacg ctccgtgcgc
     6301 ggcagggcgc tttggggcgg tgtgtcgtgc aggcgcagca gaagaccttg cgcaactttg
     6361 gccggctcca gcgtgcgggc cagctccagt gtctggttga aaccgcgctc ttgcaagcgc
     6421 tggctggaga gcggcatggg aatcagcagg tcggcctggt cgagcgcggg ctctacccag
     6481 ggtgcgctgc gcatcagcag ggcgagggtg cgggcccatc cggtgtgctg gtggaacttg
     6541 aaatcgacga ccagtcccga ccagggatag gcataggcca ccgccgccag gcactggtcc
     6601 actggcggtg ggtgtagcag gcagtcgccg caccgctgca ctcctgccgg gacgggcagg
     6661 gcgcaggtga tgcagcgggg cacgggctgg gcgaatgccg ccacgcaggc gtcacacacc
     6721 ggctgcgctg gccaggtgtg gcaaatggca cactggctgg gcaggcgctg gcttatccct
     6781 tcaatcagcg gacggaacat cgggccaata tactcgccgg tccgcccttg gtttgcttcc
     6841 ctaccccgca tgcgtacaga acgcccccct acgattgacc cggttgccgc tgcacgctgg
     6901 gagcagcgcg tgcccgccga gtcgccctgg ttgcatgagg aagtggctcg ccgcatggaa
     6961 gaccgtctgc aatggatcgt cagccaaccc aggcgctggg cccactgggc gccccttcag
     7021 ggcggattgc aggcgcaggc gctgctggcc aagcggtacc caaaatctga gtgttttgtg
     7081 gtcgaagccc ccgtcgaacg tgtacaggct gctcgtaaat tgatagcaaa atcccggtgg
     7141 aatccgtccc aatggggatc cgccaaggtg cggtttgaag tgccggcgga gccggtgcaa
     7201 atggtgtggg ccaacatggc gctgcacatg gcgcccgacc cgatggacct gatcgcccgc
     7261 tggaaccagg tgctggacgt ggatggtttt ctgatgtttt cctgtctggg gccggacacc
     7321 ttgcgggaac tgcacggcct gtaccgcgcc atgggttggg cgccgccaac acacgctttc
     7381 accgatatgc acgactgggg tgacatgctg gttcaagccg ggtttgccga gccggtgatg
     7441 gacatggagc gcatcagctt gtcgtattct tcaccgcaaa gcctgctcga agagttgcgg
     7501 ggactgggcc gcaatctgca tcatgggcgg tttgcggggc tgcgcgggcg gacctggcac
     7561 cagagtttgc aggatgcgat agggcgcgaa ttggccgatc cggccgaggg tgggcgactg
     7621 aaactcacgt ttgaagtgat ttacggacat gctttcaagc cgaaagcgcg catgccggtg
     7681 agtccgcaaa gcagtatcac cctgcagcag atgcgcgggg cattgggact gaatcacgaa
     7741 gggaagggga cgaaatgaca ggttttcagg cggagttgcg ttggggtttg atggtggtgt
     7801 gcctgttgat ctttggattg gccttcgcgg tcatggtcgt atcggcctgg gtgcagcacc
     7861 gccgtggcgc caaggaaacc accaatttcc atggttctgt ggccgttgag gtgtgttggg
     7921 cgctggcgcc gctggtcatc gtgatgcaac tggtgtggcc caccgtgcgg tcggtgctgg
     7981 ccccctagct gaaaaagacc tggagtgtgg gctcgtgctt cgggtttcgc ccgatcaaag
     8041 cagcttctta tatccggtaa actcgcgggg ctgtttgcgg taaggcaaag aacagcaaga
     8101 ataaaaagga tttaggttta tgcaaggctt gaagaaaacg gtcttgggtg ccctcgtggc
     8161 cctggggcct gcagcggtat gggcgggtta cgagttcagt ttcccgaaac ccgtaacgcc
     8221 catcgcgcac gagacactcc atgtgcacaa cttgttcatg gtgattgcgc tgaccctgtt
     8281 ttttggtgtt ctggcgctca tgttgtattc cttctggaag caccgcaagt cggtgggcca
     8341 caaggccgct gatttcaccg cgcccaaatc ccgcaagcaa tggctctgga ccttgtttcc
     8401 gtttgcagcc ttgctggttc tggactacgg cgtcttcggc atcccggctt accacgcggt
     8461 gctgatgtat gaagacacca agaccgatgc cgagctggtg gtcaaggtga ctggcagcca
     8521 gtggctgtgg gagtacgagt tcccggccga aggcgtcaag ttctacagcc gcctggccac
     8581 tccgcgtgcg cagatcctga atgagcagcc caagggcgag cactatttgc tggaagtgga
     8641 caacccactg gtgttgcctg tgggcaagaa aatccgcttc atcacgactt ccaatgacgt
     8701 gatccacagc tggtgggtgc ctgcatttgg tgtcaagcgt gacgcagtgc ccggtttcct
     8761 gcgcgagttc tgggccaaga ttgacaccgt gggtacttac cgcggccagt gttccgagtt
     8821 gtgcggcaaa gagcacgcct acatgcccat cgtggtgaat gtggttcccc aggcagagtt
     8881 tgatagctgg ctcaagagca aaaaaacgcc tctttgatgc ccgtccattc tcatagctta
     8941 cagatatcaa gatagaagga gttgcccatg gcacacgatt tggcacatga cctgccccat
     9001 gccgacggac acgatgcgca ccatccgtat ggtggggcaa tgcggtggat tcaaaccacc
     9061 aaccacaaag acattggcct gatgtacctg attttcggta tcaccatgct gttgatgggt
     9121 ggcgcgatga ttctgggcgt gcgcgccgaa ttgttccgcc cgggcctgca gctcatgcaa
     9181 cccgagttct tcaaccagct gatcacgctg cacggtctga tcatggtgtt cggttttgcc
     9241 atgccggtag ccaccggatt ggccaactac atgctgccca tggttctggg tgcacccgac
     9301 atggcattgc cccgtctgta caactggggt ttctggattt tgccgcctgc tgccgtaatg
     9361 ctggtattgc ccttcttcct gggcctggtc ggcattggcc ctggtgcggt ggacaccggc
     9421 tggaccatgt acgcaccgct gtccgtgcaa agcggctggg gaatggactt cgcgattttt
     9481 gcagtccaca tgctgggtgt gtcgtcgatc ctgggttcca tcaatgtgat cgtcacgatc
     9541 ctgaacatgc gcgcgcccgg catgaccctg ttcaagatgc ccctgtttgc gcacggcttc
     9601 ctggtaacgg ccattttgct gatcaccatc atgccggtct tcgccggtgg cgtgaccatg
     9661 gtcctgatgg atcgccactt tgatacccac ttcttcaatg ccgctggcgg cggtgacccc
     9721 gtactgtggc aacacatttt ctggttcatg ggtcaccctg aggtgtatgt gctgctgttg
     9781 cccatcactg gcatggtgcc tcacgtactg acagcgttct cgcgcaagca gacctatggc
     9841 taccgtgcac aggtttactc catgtgggcc atcggtgttt tggccatggt agtgtgggcg
     9901 caccacatgt tcaccagcgg tatgtccacc ggtggccagc tgtacttcat gtacagcacc
     9961 atgctgatct ccctgccact cgccgtcatg ttcttctgct ggatcgccac gctgtggcgc
    10021 ggctcgctgt ccttcgagac acccatgttg tttgcggtcg gattcatcat cctgttcgga
    10081 tggggtggtc tgacgggctt gatacttgcg gatgctgcgg cagatcccca gtaccacaac
    10141 gcttacttcc tggtggcgca tttccactac gcgctgtacc ccaccacggt gatgggtgcg
    10201 atggctgcca tctactactg gctgcccaag tggactggcc acatgctcaa cgagcgcatg
    10261 ggtcgcattc acttctgggt ggtgatgatt ggcttcaacc tgacgttcat tccccagttc
    10321 atggtgggtc tggccggtat gccacgccgt attcctgact atccgctgat gttcaccgag
    10381 tggaatatgt tgtccaccgt gggtgccttc atcctgggcg cttcgcacct gatcttcatc
    10441 tacaacgtgt ggtccaacat ccgtggtggt gacaaggcag aagccaaggc ctgggaaggt
    10501 gccgaaggtc tggagtggac tgtgccgtct cctgcgccct accacacctt tgaaaagcaa
    10561 cccaccattg agtgaatgcc ggaagcgaaa caaccatgag tgcacctgac atgcaaccca
    10621 atccaaccga cgccgatgtg tgggcccagc gtcgccgcaa caatgtccgg ttgggatggg
    10681 cgctgggtgc agcggccgtg gcgatctttc tgattgccgt cttgaagtac cgcccactgt
    10741 gagcgccgaa gcatctactg cattgcagca ggacaaccgc aggttgggct ttcgcctgtt
    10801 gctggtggca attgcttttg gcgggttggg cttcgccatg gtgccgctgt acgacgtgat
    10861 ctgccgcatc accgggctca atgggcgcac caatgccact gccattgcag cggacaagaa
    10921 cacccaggtg gacatgacgc gcatggtcaa tgtggagttt ttgagccaca ccatgcctgg
    10981 cgtggggctg gagttcaagc cggagcagtt caccatgcgt gtgcatccgg gtgaggtcat
    11041 gcacacgacc tatacggtga ccaacaccag caaggaagtg tttgtcggtc aggcggtgcc
    11101 cagtgtcacg cctgcggtgg cagcgcccta cctgcaaaaa atcgagtgct tctgctttga
    11161 ccagcagaca ttccagcccg gcgaggttag aaccatgccg gtggtatttg ttatcaagcc
    11221 cgacatgagc cgggaattgg gtaccgtgac gctttcctat acgtttttcg aagcgccgaa
    11281 atcacggatt tgaattgaca ttatttgact gctagaagga acgaaccatg actccatcgc
    11341 caacgccgca atcccagggg cactacttcg tcccggcggt cagcaactac tccacgttcc
    11401 tttcggccgg tattttcatg ctggccctgg gctttatctt caagctcaat ggtgtgccgg
    11461 ccggcacctg gagcatgctg ttcggcgcag ccctgattct gtatgtgatt tttggctggt
    11521 ttggcgaggt gatcagcgag aacgtcagcg gtgtctacac cctctgggaa gaccgttcct
    11581 accgcattgg catggtctgg ttcatcttct cggaagtcat gttctttgcc tgtttcttcg
    11641 gcgtgttgta ctacatccgc cgcattgcat tgccggagtt gggtggctat gagcaggcgt
    11701 acacacccta cgccggtttc accggtgcct ggccctccag cggacctctg ggtgagactt
    11761 tcacccccat gaaggcgtgg ggcattcctg ccctgaacac catgttgctg ctgacttccg
    11821 gcgcgaccct gacctgggcc cactggggcc tgatcaagaa caagcgcagc caactcgtct
    11881 ggggcttggc gctgaccgtg attctgggcg ctgtcttcat gggcttccag gcctatgaat
    11941 atgcacacgc ttacagcgaa ctgggtctga ccctgggtgc tggcgtgtac ggcgccactt
    12001 ttttcatcct gaccggtttc cacggcatgc acgtgaccct gggaaccatc atgctgatcg
    12061 tcatgtgggc gcgcagtctg aaaggccact tctccgagca caaccacttt gcctttgaag
    12121 ccgtggcctg gtactggcac tttgtggacg tggtctggct gatcgtgttt gtgtttatct
    12181 actggatctg atcaaccggg attggcgcag caggggccta tttcagaccc cgctgcggga
    12241 tgatgccaaa gtaaaatccc gccaacagca tcaggaacag gccgatcgac agggccaccc
    12301 gcacagtcag cgcctggacc atccggtttt tatcgccctc cttgcgaaac aacagtgaca
    12361 acgcactgaa caggctggcc agaatgaggc ccagaatggc gaggatgaag atttttgtca
    12421 gcatggcaaa gggggaaaag tatttcaccc cattatgcaa ttgagcctca ccccatgcaa
    12481 aagctgcgat ttgaaccccg agtgattccc acagtagcca cagtctgcct gctggccctg
    12541 tttatccatc tggggaattg gcaggcgggc aaggcagaac gccgcgcagc ggaaatggcc
    12601 cagcacagcg agcgtgcccg attgggccct tacgccatcg gtgccgtgct ggtggatccg
    12661 ggtgtcctgc aggacgctcc cgtgacggta cgtggcgtgt acgagcccga agcccagttc
    12721 tttgtcgaca accgccagga agacggcaaa cccggtgtgc atgtggtgac tccgctcaag
    12781 attgagggca gcgaaacccg catactggtc aaccgaggtt gggtgggatg gacccagggt
    12841 cggcaggtgt tacccaaagt cgctaccatc atcggcgttg tgcaggtgag cggcgtggcg
    12901 acggtaccgg tccacaaggg gttttttctg atgcccgagc gtgccgagag cctgcccagg
    12961 ctgtggagcc ggctggactt gtcgcgcttt gccggcgagc acagctaccc ggtgcagccc
    13021 gtggtggtgt tgcaggcccc tgaggatgcc aaggatggcc tggtgcgccg ctggcaacca
    13081 ccggaagacc gcgtcggcat gcaccagagt tattcctacc aatggtatgg aatggcactg
    13141 gccctgctgg ttttttatgg ggtcgccagc gtgcgtaccc ggaaaagttc atgaccaaat
    13201 tgcatcttga cgagcccctg gggctcgccg ttcattccct tccttcgccc caggacactg
    13261 cagcggagcc atcgtctgtg cgcgggcgct ggaagctgct ggccatcatg gtgttgtgct
    13321 cgttgccggt ggtggcgtcg tatctggcct attttgtggt gcggccgcag ggcaaggcag
    13381 gctacggtga attgatcagt ccggtccgcc cgatcgccca gcaaaccggg ctggcgctcg
    13441 atggcaagtc ccaggcactg gcatccctca agggccaatg gctgctggtc agtgtaggac
    13501 cgggtagctg cgcgcaggat tgccagcagc ggctgttcct gcaacgccag ttgcgggaaa
    13561 cgctgggcaa gggcaaggag cgggtggact gggtctggct ggtcagtgac gtcgcgccgg
    13621 tcgacaccag catgcgcaaa cccctgggtg atgccgtggt gctgcgggtc gatccttcgg
    13681 tcgtacagga ttggctgggt gttgatgcag ccagcgcggt caactatctc tttgtggtgg
    13741 acccgatggg caacaccatg atgcgcctcc cttcccagtt tgatggtgcc ggtgccgcca
    13801 aggcgcggcg cgacctggag cggctgttgc gtgcttccgt gtcctgggac acgccgggac
    13861 ggtaagcagc atggcatcga atccggagct ctacaacctg tccccgctgg ggctgttgct
    13921 gctggtcggg ggcgctctgg cgctgctgcc gctggcctgg gtatggctgc gcaaccgccg
    13981 ggcgagcccg gcggtgcgct ggcaggcgct ggccttgctg acgctttttc tgacctttga
    14041 tctggtgctg tttggcgcgt ttacccgttt gaccgattcg ggtctgggtt gcccggactg
    14101 gcccggctgc tatggcagcg ccagtccgct gggagcccat gcggatatcg ccgcggcgca
    14161 gactgccctg ccgaccgggc cagtcacgca cggcaaggcc tgggtggaaa tggtgcaccg
    14221 gtatctggcg accagtgtgg gcgcgctgat cgtggtgctg acccttatcg cctg
//
LOCUS       DAOSJM010000010        13553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4640_length_13553_cov_4.650985, whole genome shotgun sequence.
ACCESSION   DAOSJM010000010 DAOSJM010000000
VERSION     DAOSJM010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13553
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4640_length_13553_cov_4.650985"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PKX82_00825"
     CDS             complement(<1..180)
                     /locus_tag="PKX82_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HOF50815.1"
                     /translation="MSLANLVNHQIRLAARPVGMPKASDWSHTTEAVAEPAEGGVTLK
                     TLSLSLDPAMRGWMNE"
     gene            complement(277..888)
                     /locus_tag="PKX82_00830"
     CDS             complement(277..888)
                     /locus_tag="PKX82_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOF50816.1"
                     /translation="MEAPEPALRHGLYEDLQALVTGTLFVSLGVVMFGHTGLLTGGTA
                     GVAFLIHYATGWNFGLVFFAINLPFYGLALQRMGKRFTLKTFAAVGLMAAFSNLVPQM
                     VQFGELNPLFTAVFGGLLMGTGMLILFRHRSSLGGFNVLVLYLQERFGWRAGRIQMAL
                     DCSIVLCAFALTDWQHIALSVLGAVALNQTLATNHRAGRYMAM"
     gene            complement(888..1418)
                     /locus_tag="PKX82_00835"
     CDS             complement(888..1418)
                     /locus_tag="PKX82_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOF50817.1"
                     /translation="MTTPQFSTTPVAWDDALLLDLKPMDDDHQEIVALLAEVVRADDD
                     YLMPRWSALIAGMQRHFDEEDRWMQDTGFAADNCHSSQHKIILQILREGEARGHNGQH
                     DVVRQMAYELGVWLPHHVEAMDSSLVIHLRSVGFDPVTRTVPNGYQATTESIHGCGGS
                     TCGDTATATAGEVTPA"
     gene            1543..2286
                     /locus_tag="PKX82_00840"
     CDS             1543..2286
                     /locus_tag="PKX82_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HOF50818.1"
                     /translation="MTASFAPPVIDRSDALGECLQRQGYAVVGAAQVAELAGVSLAEL
                     GSWQAQWDGLPPDLYLRDGGRYRRRRHSSFVVNGDAVTQVAHRPHWQPVEYNALHGGM
                     QRHFEPMLGAMVGHPAWQKLLVWLGQVSSGLKGAQPWYAEAHQFRIDTTDGIGRPTPE
                     GAHRDGVDLVAVFMVDRQGVKGGETRVFEAAGPNGQRFTLSEPWSVLLLDDSRVIHES
                     TPIQPLTQGGHRDTLVVTLRSGGFQDEVL"
     gene            complement(2326..3561)
                     /locus_tag="PKX82_00845"
     CDS             complement(2326..3561)
                     /locus_tag="PKX82_00845"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOF50819.1"
                     /translation="MTPLANDTQYISPDCLDGLQRSGLQPLHLAGRTLLPIVQGGMGI
                     GISGNRLAGSVAAVGGVGTLSAVDLRRHHPDLMDQTRELPPGEAAKDGINQANLEALG
                     REIKAARERTGGKGLLAVNVMRAVAEYAACVTKALESGIDAVVVGAGLPLDLPDLAKD
                     HPKAALIPILSDARGVQLLVKKWERKKRLPDAIVIEHPRLAGGHLGAAKIADLQDTRF
                     DFENVIPQVRQFFKDAGYEKDIPLIAAGGINSFEDIARLQAMGASAVQLGTAFAVTEE
                     SDAHAEFKRVLAEARPEDMVEFTSVAGLPARAVATPWLRAYLKIEPRLQAVAHVKARC
                     TKSFDCLAQCGLRDGLQGWGQFCIDNQLAAALRGDLKKGLFFRGAGTLPFGDQIRSVR
                     ELMQRLLTPATGSYTLQQA"
     gene            3667..4707
                     /locus_tag="PKX82_00850"
     CDS             3667..4707
                     /locus_tag="PKX82_00850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF50820.1"
                     /translation="MKRLVKGLVGMSSLRDRDALDNALVQLVGEHSDTGMEAVHLVRI
                     VGETIDPRCLTLTTWGGQSAVREQDMVWADWSLLPRLDAYPNRLASVVQRTISCSNHA
                     PFTAVFPIEQTPGVANLLVEIVSPQPLSASALATVESAVENYKNLLGMLDYGEKDALT
                     ELLNRKSFDGAFFKAAETEHLKLEEIEHERRTVARDAGHWLAVLDIDFFKRVNDNFGH
                     LIGDEVLLLMARLMRSNFRFHDQLYRFGGEEFVILMRCPNAHDAHIALERLRTRVESH
                     TFPQVGHITVSIGSTCLQKNDTPGAAFGRADKAVYYAKEHGRNQICSYEELVQSGALE
                     QPKSESMDVDLF"
     gene            complement(4715..5902)
                     /locus_tag="PKX82_00855"
     CDS             complement(4715..5902)
                     /locus_tag="PKX82_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HOF50821.1"
                     /translation="MSREVVVVSAVRTAIGTFGGSLKDIPPTELGAQVVRESLKRAHV
                     DGKDVGHVVFGHVVNTEPKDMYLSRVAAINGGCGEGTPAFNVNRLCGSGLQAIVSASQ
                     SILLGDTDIAIGGGAENMSRAPFASLNMRWGARMGDTKMVDMMIGALHDPFHTIHMGV
                     TAENIAAKWGISREEQDATALESHNRAQRATEAGYFKSQIMPVMLKSKKGDVAYEVDE
                     HFRPNCKLEDMSKLKPAFLKENGTVTAGNASGINDAAAAVVLMEAGVAKARGLKPLAR
                     LVAYAHAGVDPKYMGIGPVPATQLALKKAGLTVKDLDVIEANEAFAAQACAVSKDLGL
                     DPAKVNPNGSGISLGHPIGATGALITVKALYELERINGRYALVTMCIGGGQGIAAIFE
                     RTA"
     gene            complement(6002..6754)
                     /locus_tag="PKX82_00860"
     CDS             complement(6002..6754)
                     /locus_tag="PKX82_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF50822.1"
                     /translation="MKHLTILTGASRGMGLAMAQQLMGANARLLCLSRHTNSALGQEA
                     ARTGTQLEQWTADLAESAPVAERLRLWLQALDPATLASATLINNAGVISELVPLRQAQ
                     ADDLAHALRVGLEAPMALTAAFLSATQTWPLPRKVLNISSGLGRRAMASSSAYCAAKA
                     GMDHFTRCLALEEALVPHGAKVCSLAPGVIDTDMQSQLRSADASSFPDQSAFAGMHSN
                     GQLTSTEDAARRVLAYLQRADFGSQSVADVRG"
     gene            complement(6751..8460)
                     /gene="recJ"
                     /locus_tag="PKX82_00865"
     CDS             complement(6751..8460)
                     /gene="recJ"
                     /locus_tag="PKX82_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOF50823.1"
                     /translation="MKIIPRDIPPRAVWTLEQAGIHPLLARLYAARGVTSTSELDDAL
                     GKLLPPSGMLGTDRAAVLLADAMAQNQRLCVVADYDCDGATACAVALRGLRLLGAKNV
                     SYLVPDRVVDGYGLTAPIAQRVKDQGADALITVDNGIASVEGVAKAKALGLQVLVTDH
                     HLPAATLPDADVIVNPNQPGCSFASKSIAGVGVMFYVLLALRAELRQRGMFDAATQPK
                     LDTLLPLVALGTVADVVKLDANNRRLVAQGLKRVRALAMPAGLASLFVASGRNASTAT
                     TFDFGFALGPRINAAGRLADMTLGIECLLTDDVARADELAKMLDGINRERRVIEGDMR
                     EQAMMIAESLFDEGDEPPPAICVFDPDFHEGVVGIVASRIKDKLHRPAFVFAASAAPG
                     KEHELKGSGRSIPGFHLRDALDLVAKRYPGVLLRFGGHAMAAGCTINEERLDDFEQGL
                     NEVAQEWLDAATLTRRLETDGPLEPQYLRVDLVDTLHKEVWGQGFAAPTFSEEVEVIS
                     QRLVGEKHLQLKLKHQGTPVDGIWFGHTEQLPARVTLAFRLDADEWNGVRRVRFLVEG
                     AEA"
     gene            complement(8457..9392)
                     /locus_tag="PKX82_00870"
     CDS             complement(8457..9392)
                     /locus_tag="PKX82_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50824.1"
                     /translation="MHLVIDHAAPGGANCRVAAAKLQLPNLARLLKSMAPGQRWAGSP
                     DTMTPVGEQVRAAAMGLRAADGLIPWAALDAQRLGLASESGAQGWAWITPCHWKVNAD
                     HVEMLDPADLAPSDAETTVLLEAMRGYFAEDGIALHPLSAGTWLAHGAVFKDLPTASL
                     ERARGARIDTWMPRQTQASPLRRLQNEMQMLLYTHPMNDARAAQRRTTVNSFWVSGTG
                     DLPQSYRADAALPPYRTLDTLRTAALRDDAALWVQAWQTLDTTELAAVDASTLTLTLC
                     GEHGAQTFAPATGSWLTRLRRRLAPPSPNTLLAAL"
     gene            9459..10715
                     /locus_tag="PKX82_00875"
     CDS             9459..10715
                     /locus_tag="PKX82_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOF50825.1"
                     /translation="MRVPYELLIGWRYTRAGRSTRRNGFISFISGVSMLGIALGVAAL
                     IIVLSVMNGFQKEVRDRMLGVVSHVEVYAAGGQALPDLALTLQQARSHPQVVGAAPFI
                     TAQALLARGEDMKGIMVRGIDPALEHEVTDLAGGAQAAVLERLVPGEFGVVLGSELAR
                     ALGVRTGDRVTLVAPSGQVTPAGVVPRLKQMTVVGTFDSGHYEYDSAMVFAHWEDTAR
                     IFRLEGPTGVRLKLRDLHQARTVADELSRTLTGEVAIRDWTRQNRTWFAAVQVEKRMM
                     FIILTLIVAVAAFNLVSTLVMTVTDKRADIAILRTIGASPGSIMGIFVVQGAMVGVIG
                     TLAGLALGLGVAFNIDVIVPALEQLLGASFLPKDIYLISRMPSDPQSSDIVPIAVISL
                     VMAFVATLYPSWRASRVNPAEALRYE"
     gene            10708..11385
                     /gene="lolD"
                     /locus_tag="PKX82_00880"
     CDS             10708..11385
                     /gene="lolD"
                     /locus_tag="PKX82_00880"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041792630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HOF50826.1"
                     /translation="MSEILLQCRGLTKRFQEGRLDVTVLQGVDLEVRRGDTLAIVGAS
                     GSGKSTLLHLLGGLDAPTSGTVTLCGQALAQLSPAAQGRLRNQHLGFVYQFHHLLPEF
                     SALDNVAMPLTIRRVDPAESAQAAMKMLASVGLAGRAHHRPAELSGGERQRVAIARAL
                     VTRPDCVLADEPTGNLDRATADGVFELMLQLARDQGTAFVLVTHDTTLAARCSRQLHL
                     DSGCLVA"
     gene            11471..12673
                     /locus_tag="PKX82_00885"
     CDS             11471..12673
                     /locus_tag="PKX82_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF50827.1"
                     /translation="MNHFYKQVVGLSLGLGLALSAGAQTAKPVRIGMIGPFSGPSADF
                     GTPMLNGAKLAVDEINAAGGYLGRSLELVIKDDTANPDVGLQRSQELMKEEVVATIGF
                     CNTGVAMKSLDVFQAAKHPLIVPCATGTPITAKYPAPESYIFRTSARDAIQAPFVVED
                     IVRRGWTKVAIFADKTGYGEAGLQDVMKALAAKDLKPVHVARFDLGIKDLTADLKAAQ
                     AAGANVVFSYTVGAENAVIARDRQAMNWKVPQVGAWPLSFPFFIDGAKDAGEGALMAQ
                     TFIAEPSNERRISFLTAYARKFETKKMTVPMAAAQSYDTVYLLTYALLGIRNADLSGP
                     TIKASLENLSRVYYGVTATHEKPFSRDDKDAITSNMLVMGKVTKSGTVTFAYPEDRTR
                     NLFVQRKR"
     gene            12687..13529
                     /locus_tag="PKX82_00890"
     CDS             12687..13529
                     /locus_tag="PKX82_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOF50828.1"
                     /translation="MAWIDTHCHLDAPEFAADILEVQRRARQAGVSHCVIPAVEVSNF
                     ESVRRLAHAGNDSYCLGIHPLYVDRAVESDLLVLDAQLAQYRDDLRLVAVGEIGLDYF
                     VPELTQTPLREKQEYFYREQLKLARKHGLPVVLHVRRSADRLLKHLRDLAAGGPWHGI
                     AHAFNGSDVQAQAFVQMGFKLGFGGAVTFERALQLRRLAQSLPLDALVMETDAPDIAP
                     HWLYATAAQRGAGQAQGRNEPCELPRMGAVVAQLRGMPVDALAVATTRNACVALPKLT
                     ALLV"
BASE COUNT         2372 a         4001 c         4591 g         2589 t
ORIGIN      
        1 ttcgttcatc cagccgcgca tggccgggtc gagcgacagc gacagggtct tgagggtgac
       61 gccgccttct gcgggttcgg ccacggcctc ggtggtgtgg ctccagtcgc tggctttggg
      121 catgccgacg gggcgggcgg ccaggcgaat ctggtggttg acgaggttgg caagggacat
      181 ggagaactcc gcaagagaat atggaaagtg gcgcatggta gcgccgttct cccccaaaga
      241 tgcgtatccg aggtgacagc cctacagtcg cctgcgttac atggccatgt agcggccggc
      301 ccggtggttg gtggccagcg tctggttcag tgccacggcg cccagcaccg acagcgcaat
      361 gtgctgccag tcggtgaggg caaaggcgca cagcacgatg ctgcagtcca gcgccatctg
      421 gatgcgcccg gcgcgccagc caaagcgttc ctgcaggtac agcaccagca cgttgaagcc
      481 acccaggctg gagcggtggc gaaacaggat cagcatgccg gtgcccatca gcaggccgcc
      541 aaagaccgcg gtgaatagcg ggttgagttc accaaactgc accatctgcg gcaccagatt
      601 gctgaaggcc gccatcaggc ccacagcagc aaacgtcttc agcgtaaacc gcttgcccat
      661 gcgctgcagt gccaggccgt aaaacggcag gttgatggca aagaacacca ggccgaagtt
      721 ccagcccgtg gcgtagtgga tcaggaaggc cacgcccgcg gtgccgcccg tgagcagccc
      781 ggtgtggcca aacatcacga caccgagcga gacaaacagc gtgcccgtga ccagcgcctg
      841 gaggtcttcg tagaggccgt ggcgcagcgc tggttcgggc gcttccatca ggcgggcgtc
      901 acttcgccgg ctgtggcagt cgcggtatcg ccgcaggtgg agccgccgca gccgtggatg
      961 gattcggtgg tggcctggta gccgttgggc accgtccggg tcaccgggtc aaaccccacg
     1021 ctgcgcagat ggatcaccag cgaggagtcc atggcttcca cgtggtgggg cagccagacg
     1081 cccagctcgt aggccatttg ccgcaccaca tcgtgctggc cgttgtggcc gcgggcttcg
     1141 ccttcacgca ggatttgcag gatgattttg tgctgcgagc tatggcagtt gtcagcggca
     1201 aaaccggtgt cctgcatcca gcggtcttcc tcgtcaaagt gcctttgcat gccagcgatc
     1261 agggcggacc agcggggcat caggtagtcg tcgtcggcgc gcaccacttc ggccagcagc
     1321 gccacgatct cctggtggtc atcgtccatg ggcttcaggt ccagaagcag cgcgtcgtcc
     1381 catgcaacag gggtggtgga aaactggggt gtggtcatgg tggggttact ccgaaactgg
     1441 cgatcagcat agccagccag aggctggggt gccttgaccc aggtcacaca ggataacttt
     1501 gggccaataa tctctcactt tacctatttc ccttcaagtt ccatgaccgc atcgtttgct
     1561 ccccccgtga ttgaccgttc cgacgcactc ggtgagtgcc tgcagcgtca gggctacgcg
     1621 gtggtggggg cggcgcaggt cgccgaactg gccggggttt ccctggccga actggggtcg
     1681 tggcaagcgc aatgggacgg tttgccgccc gacttgtacc tgcgcgacgg cggccggtat
     1741 cggcgtcggc gccattcgtc ttttgtggtg aacggcgacg ctgtcaccca ggtggcgcat
     1801 cgcccgcact ggcaacccgt ggagtacaac gccctgcacg gtggcatgca gcgccatttt
     1861 gagcccatgc tgggggccat ggtggggcac cctgcatggc agaagctcct ggtgtggctc
     1921 gggcaggtga gttccggtct gaagggcgcg caaccctggt atgccgaggc gcaccagttc
     1981 cgcattgaca ccaccgatgg catcggccgg cccacccccg aaggtgccca ccgtgacggg
     2041 gtggatctgg tggccgtgtt catggtcgat cgccaggggg tcaaaggcgg tgaaacccgt
     2101 gttttcgagg ccgcaggccc caacggccag cgctttaccc tgagcgagcc ctggtcggtg
     2161 ctgctgctcg acgattcgcg ggtgatccac gagtccaccc cgatccagcc acttacccag
     2221 ggagggcacc gcgatacact ggtggttacg ctgcgcagcg ggggcttcca ggacgaagtt
     2281 ttataaaaaa taaggctgta gcccgcgtgg aataagcgct atccgctatg cttgttgtag
     2341 cgtgtaactt ccggtcgcgg gggtcagcag gcgttgcatc agctcgcgca ccgaacggat
     2401 ctggtcgcca aagggcaggg tgccggcgcc gcggaagaac agccccttct tgaggtcgcc
     2461 ccgcagcgcg gcggccagct ggttgtcgat gcagaactgg ccccagccct gcaggccatc
     2521 gcgcaggcca cactgggcca ggcagtcaaa gctcttggtg cagcgggcct tgacgtgggc
     2581 cacggcctgc agcctgggct cgatcttcag gtaggcgcga agccacgggg tggccacggc
     2641 gcgcgccggc aagccggcta cgctggtgaa ttccaccatg tcttcgggtc gggcctcggc
     2701 cagcacgcgc ttgaattcgg catgcgcatc gctttcttcg gtgacagcaa aggcagtgcc
     2761 cagctgcacc gcagaggcgc ccatggcctg caggcgggca atgtcctcga agctgttgat
     2821 gccaccggcg gcaatcaggg ggatgtcttt ctcgtagcct gcatccttga agaactggcg
     2881 cacctggggg atgacgtttt caaagtcaaa gcgggtgtcc tgcaggtcgg caatttttgc
     2941 agcccccaga tggccgccgg ccaggcgcgg gtgctcgatc acgatggcgt cgggcaggcg
     3001 cttcttgcgc tcccatttct tcaccagcag ctgcacgccc cgcgcatcgg acagaatggg
     3061 aatgagtgcc gccttggggt ggtccttggc caaatccggc aggtccagcg gcaggccggc
     3121 acccacgacc acggcatcga tgccgctttc cagcgctttg gtgacgcagg ccgcgtattc
     3181 ggctacggcg cgcatgacgt tgacggccag caggcccttg ccaccagtgc gctcacgcgc
     3241 tgccttgatc tctcggccca gcgcttcgag gttggcctgg ttgatgccgt ctttggcggc
     3301 ttcacccggc ggcagttcgc gggtctggtc catcaggtcg gggtggtggc gacgcaggtc
     3361 cacggctgac agtgtgccca cgccaccgac ggcagccacg ctgccggcca ggcggttgcc
     3421 cgagatgcca atgcccattc ccccctgcac aatcggcaac agggtccgcc ccgccagatg
     3481 cagtggctgc agcccgctgc gttgcagtcc atcgagacag tcaggactaa tatattgcgt
     3541 atcgttggct aagggggtca ttgggtcgtc taagatgttg aaacaggcag ggagacaaat
     3601 agccaaagag cttacggtca gcatggctgg cacgtattga gttgggtcaa tgggtacaac
     3661 aacaacatga aaagactggt caagggactg gtaggcatga gcagcctgcg cgaccgcgat
     3721 gcactggaca atgcactggt gcagctggtg ggtgagcatt cggatactgg catggaggca
     3781 gtgcatctgg tgcgaatcgt tggcgaaacc atcgacccgc gctgcctgac actgaccacc
     3841 tggggtggtc aatccgcggt gcgcgaacag gatatggtct gggctgactg gtccctgctg
     3901 ccgcgtctgg acgcgtatcc caaccgactc gcgtcggtcg tgcagcggac gatcagctgt
     3961 tcgaaccatg cgcccttcac ggcggtgttt cccattgagc agacgcccgg tgtggccaat
     4021 ctgctggtcg agatagtttc accccagccg ctgtcggcaa gcgcactggc caccgtggag
     4081 agtgcggtgg aaaactacaa gaacctcctg ggcatgctgg actacggcga gaaagatgcg
     4141 ctgaccgagt tgctcaaccg caagtcattt gacggtgcct ttttcaaggc ggcggaaacc
     4201 gagcacctga agctggaaga aatcgaacat gaacgccgca ccgttgcgcg ggatgcgggg
     4261 cactggctgg ccgtgctgga cattgatttt ttcaaacgcg tcaacgacaa cttcgggcac
     4321 ctgatcggtg acgaggtgct gctgctgatg gcccggctga tgcgcagcaa tttccgcttc
     4381 catgaccagc tttaccggtt cggaggcgag gagtttgtca tcctgatgcg ttgccccaat
     4441 gcgcacgacg cgcacattgc actggagcgc ttgcgtacgc gggtggagtc ccacacgttt
     4501 ccgcaggtgg gacatatcac ggtcagcatt ggctccacct gcctgcagaa aaacgacacg
     4561 ccgggcgccg cctttggccg ggctgacaag gcggtgtatt acgccaagga gcatggccgc
     4621 aaccagatct gcagctacga ggagttggtg cagtctggtg cgctggaaca gcccaagtcc
     4681 gagtcgatgg acgtggactt gttctagaaa aatatcaggc ggtgcgttcg aagatcgctg
     4741 cgatgccctg cccgccaccg atgcacatgg tcaccagggc gtagcggccg ttgatgcgct
     4801 ccagttcgta cagggctttc acagtaatca acgcaccggt tgcgccgatg gggtgaccca
     4861 gcgaaatgcc cgagccattg gggttgactt tggcggggtc caagcccagg tccttgctga
     4921 cggcgcaggc ctgcgccgca aaggcttcgt tggcttcaat cacgtccagg tctttcacgg
     4981 tcagaccggc ctttttgagg gccagttgcg tggcaggtac cgggccaatg cccatgtact
     5041 tggggtccac gccagcgtgg gcataggcca ccaggcgcgc caggggcttg aggccacggg
     5101 ccttggccac gccagcttcc atcaacacca cggctgcagc ggcatcgttg atacccgagg
     5161 cgttgccggc ggtgacggtg ccgttttcct tcaggaaggc gggcttgagt ttggacatgt
     5221 cttcaagctt gcagttgggg cggaaatgtt cgtccacttc gtaggccaca tcgccctttt
     5281 tgctcttgag catcacgggc atgatctgcg acttgaagta accggcttcg gtggcgcgct
     5341 gggcacggtt gtggctttcc agggcagtgg catcctgctc ttcgcggctg atgccccatt
     5401 tggcggcgat gttctcggcg gtcacaccca tgtggatggt gtggaagggg tcgtgcagcg
     5461 cgccgatcat catgtcgacc atcttggtgt cgcccatgcg ggcgccccag cgcatgttga
     5521 gcgaggcgaa cggagcgcgg ctcatgttct cggcgccgcc gccgatggcg atgtcggtgt
     5581 cgcccagcag aatgctctgg ctggcgctga cgatggcttg caggcccgag ccgcacaggc
     5641 ggttgacgtt gaaggcgggg gtgccttcgc cacagccgcc gttgatggcc gctacgcggg
     5701 agaggtacat gtccttgggc tcggtgttga cgacatggcc gaacaccacg tggcctacgt
     5761 ccttgccgtc cacatgggcg cgcttgaggg attcgcgaac cacctgggcg cccagttcgg
     5821 tggggggaat gtccttgagg cttccgccaa aggtaccaat ggcggtgcgc accgcgctga
     5881 cgacaacaac ttcacgggac atggaacttc tcctcggatt tcagatgaat ggatggttgc
     5941 aggactgcgc ggcggagtgt gcgctccaaa agctgcgcgg gacttacggg attttgacac
     6001 gtcagccgcg tacgtcggcc acgctctggc tgccaaagtc ggcgcgctgc aggtaggcca
     6061 gcacgcgccg tgcggcgtct tcggtgctgg tcagttggcc attgctgtgc atgccggcaa
     6121 aggctgactg gtccgggaaa gaggacgcat cggcactgcg cagctggctc tgcatgtcgg
     6181 tatcgatcac gccgggggca agggaacaca ctttcgcccc gtggggcaca agagcctctt
     6241 ccagtgccag gcagcgggtg aagtggtcca tgccggcctt ggctgcgcag taggccgacg
     6301 acgaggccat cgcgcgccgg cccagcccgg aggagatgtt gagcaccttg cgcggcaatg
     6361 gccaggtctg tgtggcgcta agaaaggccg cggtcaaggc catgggcgct tcgagtccga
     6421 cgcgcagcgc gtgtgccagg tcatcggctt gtgcctggcg cagcggcacc agttcagaga
     6481 tgacgccggc gttgttgatc agcgtggcac tggccagtgt ggccgggtcc agggcctgca
     6541 gccacagccg caggcgttcg gccacgggcg cgctctcggc caggtcggcg gtccactgtt
     6601 ccagttgcgt gccggtgcgc gcggcttcct gtcccagcgc ggagttggta tggcgggaga
     6661 ggcacagcag ccgcgcattg gcccccatca attgctgcgc catggccaga cccatgccgc
     6721 gcgaggcgcc ggtcaggatg gtgaggtgtt tcatgcctca gctccttcga ccagaaagcg
     6781 tacccggcgc acgccattcc actcgtcggc atcgagacgg aacgccagcg tgactcgggc
     6841 gggcagttgc tcggtgtggc caaaccagat gccatcgacc ggcgtgccct ggtgtttgag
     6901 cttgagctgc aggtgctttt cgccgaccag acgctgggag atgacctcca cctcttcact
     6961 gaaggtgggc gcggcaaaac cctgacccca cacttccttg tgcagggtgt ccaccagatc
     7021 gacgcgcagg tactgtggct ccagcgggcc atcggtttcc aggcgccggg taagggtggc
     7081 ggcgtccagc cattcctggg ccacctcgtt gagtccctgc tcgaaatcgt ccagccgttc
     7141 ctcgttgatg gtgcaacccg ctgccatggc gtggccgcca aagcgcagca gcacgccggg
     7201 gtagcgtttg gccaccagat cgagtgcatc acgcaggtga aagcccggaa tcgagcggcc
     7261 cgagcccttg agctcgtgct ccttgccggg tgcggcgctg gcagcaaaca caaaggccgg
     7321 gcggtgcagc ttgtccttga tgcgcgaggc cacgatgccg accacgcctt catggaaatc
     7381 ggggtcgaac acgcagatgg ccggtggcgg ctcgtcgcct tcgtcgaaca ggctctcggc
     7441 aatcatcatg gcctgctcgc gcatgtcgcc ttcaatcacc cggcgctcgc ggttgatgcc
     7501 gtcgagcatt ttggccagtt cgtcggcgcg ggccacatcg tcggtcagca ggcattcgat
     7561 gcccagcgtc atgtcggcca ggcggcctgc ggcattgatg cgcgggccca gcgcaaagcc
     7621 gaagtcgaag gtggtggcag tgctggcgtt gcggccagat gctacaaaaa ggctagctag
     7681 tcccgcaggc atggcgaggg ctcgaacccg ttttaagcct tgggctacga gccggcggtt
     7741 gtttgcgtca agtttcacca catcggccac ggtgcccagt gcgacgagtg gcagcaacgt
     7801 gtccagcttc ggttgtgtag ccgcgtcgaa cataccgcgc tggcgcaact cggcgcgcag
     7861 tgccagcagc acgtagaaca tgacaccgac cccggcgatg gatttgcttg cgaaggagca
     7921 gcccggctgg ttcgggttga cgatcacgtc agcatcggga agggtggccg cgggtaagtg
     7981 gtggtcggtg accagcacct gcaaccccag tgccttggct ttggcgacgc cttccacact
     8041 ggcgatgccg ttgtccacgg tgatcagcgc gtcggcaccc tggtccttga cgcgctgggc
     8101 aatcggggcg gtcagtccgt agccgtcgac cacgcggtct ggcaccaggt agctcacgtt
     8161 ttttgcaccg agcagacgca gtccgcgcag ggctacggcg caagcggtgg cgccatcgca
     8221 gtcgtaatca gccacgacgc acagccgctg gttttgcgcc atggcatcgg ccaacagcac
     8281 ggcggcgcgg tcggtgccca gcatgccact cgggggcagc agcttgccca gggcatcgtc
     8341 aagttcggag gtagaggtga cgccgcgggc ggcatacagg cgcgccagca acggatggat
     8401 gccggcctgt tccagtgtcc agactgcgcg tggcggaatg tcgcggggaa tgattttcat
     8461 agtgcagcaa gcaatgtgtt cggagaaggc ggcgccagtc gccggcgcag ccgggtcagc
     8521 caggagcccg tggcaggtgc gaaggtttgc gcgccgtgtt cgccgcagag ggtgagggtc
     8581 agcgtgctgg catccactgc ggcgagttcg gtggtatcca gtgtctgcca ggcctgtacc
     8641 cagagcgctg cgtcgtcgcg cagggcggca gtccgcaggg tgtccagcgt gcggtagggt
     8701 ggcagcgcgg catcggcacg gtagctttgc ggtagatcac cggtgccgct gacccagaag
     8761 gaattcaccg tggtgcggcg ctgtgccgcc cgggcgtcgt tcatggggtg ggtgtagagc
     8821 agcatctgca tctcgttctg caggcggcgc agggggctgg cttgcgtctg gcgtggcatc
     8881 caggtgtcga tgcgggcacc gcgcgcacgc tccagcgagg cggtcggcaa gtccttgaat
     8941 acggcgccgt gcgccagcca ggtgcctgcc gacaaggggt gcagggcgat gccgtcctcg
     9001 gcaaagtaac cgcgcatggc ttccagcagg acagtggttt cggcgtctga gggtgccaga
     9061 tctgcggggt ccagcatttc cacgtggtcg gcgttgactt tccagtggca gggggtgatc
     9121 caggcccagc cttgtgcgcc agactccgat gccagcccca ggcgctgtgc atccagcgcc
     9181 gcccagggaa tcagtccgtc tgccgcgcgc agtcccatgg ctgctgcgcg tacttgttcg
     9241 cccaccgggg tcatggtgtc cgggctgccc gcccaacgct ggccgggtgc catggacttg
     9301 agcaggcgcg caagattcgg aagctgcagt ttggctgcag cgacccggca attggcgcca
     9361 ccaggcgctg catggtcaat aacaaggtgc atgccgccat tgtcctgcat ggatccacag
     9421 ggatgacaca atcggggaaa gtgaggttac ttatcctgat gcgtgttccc tatgaattgc
     9481 tgatcggctg gcgctacacc cgcgcaggcc gctctacgcg gcgtaacggc ttcatctcgt
     9541 ttatctcggg cgtatccatg ctggggattg cgctcggtgt ggcggctctc atcatcgtgc
     9601 tcagcgtgat gaacggcttt cagaaagagg tgcgcgaccg catgctgggc gtggtctccc
     9661 atgtggaggt ctatgccgcg ggtgggcagg cgctgcctga tctggccctg acactgcagc
     9721 aggcccggtc ccatccccag gtcgttggtg ctgcaccttt tataacggca caggcactgt
     9781 tggcgcgggg tgaggacatg aaaggcatca tggtgcgggg catcgacccg gccctggagc
     9841 atgaggtgac cgacctggct ggcggcgcac aggcggcggt gctggagcgt ctggtgccgg
     9901 gggagtttgg tgtggtgctg ggctctgagc tggcgcgcgc attgggcgtg cgcactggtg
     9961 atcgggtaac gctggtagca cccagcgggc aggtcacgcc ggccggtgtg gtgccgcgcc
    10021 tcaagcagat gacggtggtg gggacctttg attctggcca ctacgagtac gactcggcca
    10081 tggtgttcgc ccactgggag gacacggccc ggatattccg gctggaggga ccgaccgggg
    10141 tgcgtctgaa actgcgtgac ctgcaccagg cacgcacggt ggccgatgaa ctcagccgca
    10201 cgctgaccgg tgaggtggcc atccgggact ggacccggca aaaccgcacc tggtttgcgg
    10261 ccgtgcaggt ggaaaaacgc atgatgttca tcatcctcac gctcatcgtc gcggtggcgg
    10321 cgttcaacct ggtgtcgacc ctggtgatga cggtgactga caagcgtgcc gatattgcga
    10381 tcctgcgcac gataggtgcc agcccgggca gcatcatggg catttttgtg gtgcaaggcg
    10441 cgatggtggg cgtgatcggc acgctggccg gtctggcgct ggggctaggg gtggcgttca
    10501 atatcgatgt catcgtgcca gccctggaac aactgctcgg tgccagcttt ctgcccaagg
    10561 atatttacct gatcagccgc atgcccagcg acccgcaaag cagcgatatt gttccgattg
    10621 cggtgatttc cctggtgatg gccttcgtgg caaccctgta ccccagctgg cgtgccagcc
    10681 gggtgaaccc agcggaggcc ttgcgttatg agtgaaatat tattgcaatg ccgtggcctg
    10741 accaagcggt ttcaggaggg gcggctggat gtgaccgtgc tgcagggcgt ggacctggag
    10801 gtacggcgcg gcgatacgct ggccatcgtt ggcgcatcgg gttcgggcaa gagcacgctg
    10861 ctgcatctgc tgggcggtct cgatgcgcct accagtggca cagtgacgct gtgtggccag
    10921 gcgctggcgc agttgagtcc tgctgcgcag ggccgcctgc ggaatcagca cctggggttt
    10981 gtgtaccagt ttcaccacct gttgcccgag ttcagtgccc tcgataacgt cgccatgcca
    11041 ttgacaattc gccgtgtaga ccccgcagaa tctgcgcagg cagctatgaa aatgctagca
    11101 tctgtagggt tggcagggcg tgcacaccac cgtccggcag agctttcggg tggggagcgc
    11161 cagcgcgtgg ctatcgcccg ggccttggtg acccgcccgg actgtgtgct ggcagacgaa
    11221 cctacgggca atctggaccg tgccacggcc gatggggtgt tcgaattgat gctgcagttg
    11281 gcgcgtgacc agggcacggc gtttgtgctg gtgacccacg atacgacatt ggcggcgcgc
    11341 tgcagccggc agctgcatct ggattcgggg tgcctggtgg cttgagatgc gtcagtggcc
    11401 cgaaagggtt ggtgcacata ttccggtaac cggtgtagtc ctgtgctacc tgtatttttt
    11461 tgagagaacc atgaatcatt tctacaagca ggtcgttggg ttgtcgctgg gtttgggttt
    11521 ggcgctatcg gcgggtgcgc agaccgcgaa gcccgtgcgt atcgggatga tcgggccctt
    11581 ctcgggaccc tcggctgact ttggtacgcc catgctcaat ggggccaaac tggcggtgga
    11641 cgaaatcaac gcggctggcg gttacctggg gcgctcgctg gagctggtca tcaaggatga
    11701 cactgccaac cccgatgtgg gtttgcaacg gtcccaggaa ttgatgaaag aggaggtggt
    11761 tgcaaccatc ggcttttgca acacgggtgt ggcgatgaaa tcgctggacg tgtttcaggc
    11821 cgccaaacat cccctgatcg tgccgtgtgc caccggcacg cccatcacgg ccaaataccc
    11881 tgcgcccgag agctacatct tccgcacatc ggcgcgggat gccattcagg cgccgtttgt
    11941 ggtggaggac attgtgcgca ggggctggac caaggtggcc atttttgcgg acaagaccgg
    12001 gtacggtgag gccggcctgc aggatgtcat gaaagcactg gcggcgaaag acctcaaacc
    12061 cgtgcatgtg gcgcgtttcg atctgggaat caaggatctc accgcggacc tgaaggccgc
    12121 ccaggcggcc ggggcgaatg tggtgtttag ctacaccgtg ggtgccgaga acgccgtgat
    12181 tgcacgggat cgtcaggcca tgaactggaa ggtgccgcag gtgggtgcat ggcctttgtc
    12241 tttcccgttt ttcatcgatg gtgcgaagga tgcaggcgaa ggtgccctga tggcgcagac
    12301 ctttattgcc gaaccaagca acgagcgacg tatttctttc ctgaccgcgt atgcacgtaa
    12361 attcgaaaca aagaagatga ccgtgcccat ggctgctgcg cagtcgtatg acacggtgta
    12421 cctgttgacc tatgccctgc tgggcatacg caacgcagac ctgtcaggac cgaccatcaa
    12481 ggcgtcactg gagaatctga gccgggtgta ttacggcgtg accgccactc acgagaaacc
    12541 cttcagccgg gacgacaagg acgccatcac cagcaatatg ctggtgatgg gcaaggtgac
    12601 aaaaagcgga accgtgacat ttgcctaccc ggaagaccgg acccgcaatc tgtttgtgca
    12661 gcgcaagcgc tgacggggcc cgacacgtgg cttggattga cacccactgc cacctggatg
    12721 cgccagagtt cgctgctgat atcctggagg tgcagcgcag ggcgcgacag gccggcgtgt
    12781 cgcattgcgt gattccggcg gtagaagtgt ccaattttga atcggtgcgg cgtctggccc
    12841 atgcgggcaa cgatagctac tgccttggaa tccatcctct ctatgtggac cgtgcggtgg
    12901 agagtgatct gttggtgctg gatgcccagc ttgcacagta ccgcgacgat ctgcggctgg
    12961 tggcggtggg tgaaatcggg ctggattact ttgtgcccga actcacccag accccgttgc
    13021 gcgagaagca ggaatatttt taccgcgagc agctcaagct ggcgcgaaaa catgggttac
    13081 ctgtggtgct gcatgtgcgc cggtcggctg atcgcctgct aaaacatctg cgggaccttg
    13141 cggctggcgg gccatggcac gggattgccc acgctttcaa cggcagcgac gtgcaggcgc
    13201 aggcttttgt gcagatgggc ttcaagctcg gttttggtgg cgcggtgact ttcgagcgtg
    13261 cgctgcagtt gcgccggctg gcccagagcc ttccgctgga tgcgctggta atggagaccg
    13321 atgcccccga tatcgcgccg cactggctct atgccactgc agcacagcgc ggcgccggac
    13381 aggcacaggg gcgtaacgaa ccgtgtgaat tgccacgcat gggcgcggtc gtggcgcagt
    13441 tgcgtggtat gccggtggac gcattggcag tggccaccac ccgcaatgcc tgcgtggcat
    13501 tgccgaagct gacggccctg ctggtataaa tccccatggc aaaaaacgaa ata
//
LOCUS       DAOSJM010000011        13500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4660_length_13500_cov_5.168762, whole genome shotgun sequence.
ACCESSION   DAOSJM010000011 DAOSJM010000000
VERSION     DAOSJM010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13500
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4660_length_13500_cov_5.168762"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKX82_00895"
     CDS             <1..512
                     /locus_tag="PKX82_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOF50829.1"
                     /translation="IYFANHQSHADLVMIWAALPKELRTVTRAIAAKDYWTKTPFKQW
                     LTTAVFNVIYVSRDRTADEDPLEPLLEALSNGDSIILFPEGTRGHAEEPQAFKAGLYN
                     LACKFPQAVLVPAWINNVQHVLPKGEVIPVPVLCSVTFGAPIQVEPGEECKAFLTRAR
                     QAVIALRDV"
     gene            530..1519
                     /locus_tag="PKX82_00900"
     CDS             530..1519
                     /locus_tag="PKX82_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOF50830.1"
                     /translation="MYFYLKNLTPTQQVGALFVIVFGLLLLASAMAFMLSLREYTDEA
                     LAERRRKDLKSINGVIRTSWLMVLVFWIGWISGDWVALTLFGLVSFFALREFLTLSPT
                     RLGDHRSLVLAFFVVLPVQYWLVGSGHFDMFAVFIPVYVFLALPLVSALANDPLRFLE
                     RNAKLQWGIMVCIYGMSHVPALLMLKFPGYDNKNAFMVFFLVLVVQICMVTQHLASRR
                     LQRPPVAPAISQSFNWRSWGIGMAVASLCGALLAGITPWVPGTAFAFSFIACLAGSMG
                     HFVMKALKRDRGIPSWGAEGRSTTGAGGLLDRVDALCFAAPVFFHSARWYFDL"
     gene            1521..2081
                     /gene="ruvC"
                     /locus_tag="PKX82_00905"
     CDS             1521..2081
                     /gene="ruvC"
                     /locus_tag="PKX82_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOF50831.1"
                     /translation="MRILGIDPGLRTTGFGVVDVHGADVSYVASGTISTQHLDTRALP
                     ERLKVLFDGISEVVQRYQPTCASIEIVFVNVNPQSTLLLGQARGALITALVTQNLPVA
                     EYTALQMKQAVAGHGQAQKAQIQEMVKRLLALPGLPGPDAADALGLAITHAHAGKAMA
                     RLAAADGLGGVSKMGAKASYRAGRSR"
     gene            complement(2097..2396)
                     /locus_tag="PKX82_00910"
     CDS             complement(2097..2396)
                     /locus_tag="PKX82_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HOF50832.1"
                     /translation="MASISSIALSGMNAAQTQLNASAHNVANLPTKGFRRHEVVAGEQ
                     AGGGVTTQTVRSATVGSALETDVVTQLQAKHAFLANLAVFKTSNAMTGALLDDKA"
     gene            complement(2432..2608)
                     /locus_tag="PKX82_00915"
     CDS             complement(2432..2608)
                     /locus_tag="PKX82_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50833.1"
                     /translation="MLLFRFLILLCLLGSAVSFALYVGTGNPRYKDWGWKVLKWTLLA
                     AFCFFTVLILERVA"
     gene            complement(2621..3544)
                     /gene="lpxC"
                     /locus_tag="PKX82_00920"
     CDS             complement(2621..3544)
                     /gene="lpxC"
                     /locus_tag="PKX82_00920"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOF50834.1"
                     /translation="MLKQRTLKSLTQAVGVGLHSGQRVEITLRPAAPDTGIVFRRVDL
                     PQPVDIPVSATAVTDTRLASTLSNGDARVNTVEHLMSACAGLGVDNLYVDITAEEIPI
                     LDGSAASFVFLLQSAGIELQNAPRRFIRLLKPVEVREGSGANEKWARLEPYHGYKLSF
                     EIDFNHPAVDGTGQRVEFDMSTGSYSRDIARARTFGFTKDVEMMRANGLALGGGLDNA
                     IVMDDYKVLNADGLRYDDEFVKHKILDAMGDLYIVGKPLLAAYSAFRSGHALNNKLLR
                     ELLAHQDAWEIVTFEDAKLAPAGFAVPARAW"
     gene            complement(3700..4944)
                     /gene="ftsZ"
                     /locus_tag="PKX82_00925"
     CDS             complement(3700..4944)
                     /gene="ftsZ"
                     /locus_tag="PKX82_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOF50835.1"
                     /translation="MAIEMIEEEAFNQGTQIKVIGVGGGGGNAVEHMIATAVGGVEFI
                     CANTDAQALSRSTAHKTIQLGGSGLGAGSKPDKGREAAEQAEADIRNAIEGAHMLFIT
                     AGMGGGTGTGAAPIIAKVAKEMGILTVGVVTKPFDFEGGRRMSNADAGLAELEANVDS
                     LIVVLNEKLLEVLGDDVSQDEAFAHANDVLKNAVGGIAEIINVPGHVNVDFEDVRTVM
                     GEPGKAMMGTAVASGPDRARIAAEQAVACPLLEGIDLSGAKGVLVLITAAKGSLKLSE
                     SKLAMNTIRAYASPDAHVIYGTAYDDALGDEIRVTVVATGLSRQGARRTAPPLQVLRT
                     GTDNVPFHVPTLNNIAQPAGITASAGGAASGGLTQPDYGSMSTPSVWRTNRTQAAAKV
                     DALSSGGMDDYEIPAFLRKQAD"
     gene            complement(5085..6314)
                     /gene="ftsA"
                     /locus_tag="PKX82_00930"
     CDS             complement(5085..6314)
                     /gene="ftsA"
                     /locus_tag="PKX82_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOF50836.1"
                     /translation="MAKEYKDLVVGLDIGTAKVMAVVAEVMPGGELKLAGLGIAPSNG
                     LKRGVVVNIDATVASIQQALKEAELMADCKITRVYTGITGSHIRGINSSGMVAIKDRE
                     VTAADVARVVETAKAINISTDQRLLLVEPQEFIIDGQDVKEPIGMSGIRLEAKVHIVT
                     GAQSAAENIIKCVRRCGLEVEQLMLNPLASSLSVLTEDERELGVALVDIGAGTTDVAV
                     FTNGAIRHTAVIPIAGDLITSDIAMALRTPTKDAEDIKVEYGHAKQLLVDPETQVEVP
                     GLGDRGPRMLSRQALAGVIEPRIEEIFSLVNQVMRESGFEELLSSGIVLTGGSCIMPG
                     MVELGEDIFLKPVRRGVPKYSSALADMVAQPRAATVMGLLEEARLARLRGYKVAQKAG
                     SVKTAFSSVKDWFVGNF"
     gene            complement(6348..7130)
                     /locus_tag="PKX82_00935"
     CDS             complement(6348..7130)
                     /locus_tag="PKX82_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HOF50837.1"
                     /translation="MNPVATPLDVKLMNMTAMVLGLAFVVLGAVAAARWVSRLPAFDI
                     QGILVSGDVAHNNTVTLRANVAPRLSGTFFTVDLARVRAAFESVPWVRRAVVRRDFPN
                     RLRVDLQEHQPVAYWGGESDERLVNSYGEVFEANVGEVEQDALPRLHGPDGQAGDVLA
                     MYQALQDMFEQMDIPIEALDLSTGGSWRARLDTGAVIELGRGGVGDVSARVQRFLKTL
                     TQVITRYGRQPSAVEFADLRHENGYAIRLRGVSTVAADGPKK"
     gene            complement(7127..8098)
                     /locus_tag="PKX82_00940"
     CDS             complement(7127..8098)
                     /locus_tag="PKX82_00940"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOF50838.1"
                     /translation="MSRIANKIAASLGKVAVLMGGASAEREVSLMSGGGVLKALLARG
                     VNAHAFDPAERPLDDLKREGFDRCFIALHGRFGEDGTVQGALELLGIPYTGSGVMASA
                     ISMDKVMTKRIWRFEGLPTPAWKQVSSAAQTHAAFAELGAPMIVKPAREGSSIGFTKV
                     MTADQCDAAYAAAAKHDNEVLCEQFIAGDEVTCPVLGATESPRALPVIRIVAPDGNYD
                     YQNKYFTDTTQYLVPAGLPAGEEEAIQAIVCQAYQVLGCRGWARADVMIDAATRKPYL
                     LEINTSPGMTGHSLVPMSARADGISYEDLCVQLLQHTELDARASASV"
     gene            complement(8095..9528)
                     /gene="murC"
                     /locus_tag="PKX82_00945"
     CDS             complement(8095..9528)
                     /gene="murC"
                     /locus_tag="PKX82_00945"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOF50839.1"
                     /translation="MKHAIKHIHFVGIGGSGMSGIAEVLSNLGYVISGSDLSDSATLK
                     RLASLGITTYVGHAEAHVAGADAVVTSTAVQSDNPEVVRAREMHIPIVPRALMLAELM
                     RLKQGIAIAGTHGKTTTTSLVASVLAEAGLDPTFVIGGRLNSAGANARLGSGDHIVVE
                     ADESDASFLNLLPVMAVVTNIDADHMETYGHDFGRLKKAFVDFLHRMPFYGTAILCTD
                     DAAVREICEQVTCPITSYGFNEEAQVRAINVRAVDGQMHFTVQRRNGVVLPDLDVVLN
                     LPGLHNVLNALSAIAVAVELNIADAAVVKALAEFKGVGRRFQRYGDLPLPSGGTVTVI
                     DDYGHHPVEMAATLAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVIGQADAVLLG
                     EVYAAGEAPIVAADGRSLARALRVAGKVEPIFVDDIAAMPQAAVDNAQDGDVLLCMGA
                     GSIGAVPGKIVNLLQNTEHAAQKGRGL"
     gene            complement(9525..10592)
                     /gene="murG"
                     /locus_tag="PKX82_00950"
     CDS             complement(9525..10592)
                     /gene="murG"
                     /locus_tag="PKX82_00950"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOF50840.1"
                     /translation="MSTQPCALIMAGGTGGHIFPGLAVAQALRDKGWRVHWLGAPASM
                     ESRLVPARGFPLETVDFAGVRGKGLRTLVLLPLRLLKAFWQSLQVVRRVRPQVVVGLG
                     GYITFPGGLMSVLLGKPLVLHEQNSVAGMANKVLAGVADRVFTAFPKVFTKGQWVGNP
                     LRAEFLTQLAPQERFAGRSGPLKVLVVGGSLGARALNTVVPQALALIPEAQRPMVTHQ
                     AGEKQIDELRANYAAAGVQATLTPFIDNTAHAFADADLVICRAGASTVTEIAAVGAAA
                     VFVPFPSAVDDHQTSNARFLVDQGAGWLLPQSDMTPQVLADLLQKTERSALLQRGLAA
                     KNMQQLHATDAVVAACEELAP"
     gene            complement(10589..11848)
                     /gene="ftsW"
                     /locus_tag="PKX82_00955"
     CDS             complement(10589..11848)
                     /gene="ftsW"
                     /locus_tag="PKX82_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOF50841.1"
                     /translation="MSALFQTIAGWFSSRMAPAAAAALPVRLGGRGSFSATTTPARVH
                     GFDQPLVWVVVTLLLWGLVMVYSASIAMPDNPRFANYSHTHFLVRHVVSIGVAFVGAL
                     IAFQVPVQTWEKVAPWLFVASLVLLIAVLVPFIGKGVNGARRWISLGILNFQPSELAK
                     FAVLLYAADYMVRKMDVKERFFRAVAPMAVAVAIVGLLLLAEPDMGAFMVIAVIAMGI
                     LFLGGVNARMFFLIAAILVVAFSLMIALSDWRRERIFAYLDPWTEKYALGKGYQLSHS
                     LIAIGRGEIFGVGLGGSVEKLHWLPEAHTDFLLAVIGEEFGLIGVLTVIGLFLWLTRR
                     IMHIGRQAIALDRVFAGLVAQGVGIWMGFQSFINMGVNLGALPTKGLTLPLMSYGGSA
                     ILLNMIAIAVVLRIDFENRQMMHGGRA"
     gene            complement(11845..13404)
                     /gene="murD"
                     /locus_tag="PKX82_00960"
     CDS             complement(11845..13404)
                     /gene="murD"
                     /locus_tag="PKX82_00960"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOF50842.1"
                     /translation="MQDAVPDGQADAPLQPLAGQNILILGLGASGLAMARWCVRTGAA
                     SVIVADTRAAPPQLVTLQQELPQVQFVSGEFQAALVEGQSLHAVYRSPGLSPEVVAPV
                     CDTARAAGIYVGGELSLFAMALQTLRTARAYAPSVLAITGTNGKTTVTSLTWQLVAHG
                     GKPVAVAGNIGPTLLDTLSSHLDADTLPEVWVLELSSFQLDGVSNFEPTAAVVLNVTQ
                     DHLDWHGSMQAYAQAKTRIFGQHGLMVLNRDDAAVMAMLPEPVRVKLQRPQERAHTTF
                     GTDMPKRPGDYGLEDVNGMVWLVRALEADETRKKRKDDEEELHIQRLMPADALRIRGR
                     HNASNALAALALSAAAGCALGPILYGLREYRGEPHRVEPVGILNGVEFFDDSKGTNVG
                     ATVAALQGLGADRKVVVILGGEGKGQDFGPLAAPVARYVRAVVLIGRDAPLISAALER
                     TGVALADAGSMSEAVAQANQRAHAGDAVLMSPACASFDMFDNYEHRARVFVAAVKELA
                     MDAGVDMENAA"
BASE COUNT         2453 a         4386 c         4148 g         2513 t
ORIGIN      
        1 gcatctactt tgccaaccac cagagccacg ccgatctggt gatgatctgg gcggccctgc
       61 ccaaggagct gcgcactgtc acccgcgcca tcgccgccaa ggactactgg accaagacac
      121 cgttcaagca gtggctcacc acggcggtgt tcaacgtgat ctatgtgtcg cgtgaccgca
      181 ctgccgatga ggacccgctg gagccgttgc tggaggcctt gtcgaacggg gactccatca
      241 tcctgttccc cgaagggacc cgcggccacg cggaggagcc gcaggccttc aaggccggtc
      301 tctacaacct ggcgtgcaag tttccacagg cggtgctggt gcctgcctgg atcaacaacg
      361 tgcagcacgt attgcccaag ggcgaggtga ttcccgtgcc cgtgctgtgc tcggtgacgt
      421 ttggtgcccc catccaggtg gagccgggtg aagagtgcaa ggcctttctg acccgtgcgc
      481 gccaggccgt gatcgctctg cgggatgtgt agaaagcaca caagacgcca tgtatttcta
      541 cctgaagaac ctcaccccca cccagcaggt cggcgccctg ttcgtgatcg tgttcgggct
      601 gctgctgctg gccagcgcca tggccttcat gctgtcgttg cgcgaataca ccgatgaagc
      661 cttggccgag cggcggcgca aggacctcaa gagcatcaac ggcgtgatcc gcaccagctg
      721 gctgatggtg ctggtgttct ggattggctg gatttccggc gactgggtgg cactgaccct
      781 gttcggtctg gtgtcgttct ttgcgttgcg cgagttcctc acgctttccc ccaccaggct
      841 cggtgaccac cgcagtctgg tgttggcgtt cttcgttgtg ctgccggtac agtactggct
      901 ggtgggcagc gggcatttcg acatgtttgc ggtcttcatc ccggtctatg tatttctggc
      961 cctgccactg gtcagcgccc tggcgaatga cccactgcgt tttctggaac gcaacgccaa
     1021 gctacagtgg ggcatcatgg tctgcatcta cggcatgagc catgtgcctg ccctgttgat
     1081 gctcaagttc cccggctacg ataacaagaa cgccttcatg gtgttcttcc tggtgctggt
     1141 cgtgcagatc tgcatggtga cccagcacct ggcttcgcgc aggctgcagc gcccgccggt
     1201 ggccccggcc atcagccaga gtttcaactg gcgcagctgg ggcattggca tggcggtcgc
     1261 cagcctgtgt ggtgcgctgc tggccggcat caccccttgg gtgccaggga ccgcgtttgc
     1321 cttcagcttc atcgcttgcc tggctggttc catgggccac tttgtcatga aggccctcaa
     1381 acgcgatcgc ggcattccca gctggggcgc cgaaggccgg tccaccaccg gtgcaggtgg
     1441 attgctggac cgggtggatg cgttgtgctt cgcagcgcca gtgtttttcc actcagctcg
     1501 gtggtacttt gacctctagc atgcgcatcc ttggcatcga ccccggactg cgcaccaccg
     1561 gctttggcgt ggtggatgtg catggcgccg atgtcagcta cgtggccagt ggcaccatca
     1621 gtacgcagca tctggacacc cgcgccctgc ccgaacggct caaggtgctt tttgacggca
     1681 tcagcgaagt cgtgcagcgc taccagccga cctgtgcgtc catcgagatt gtgttcgtca
     1741 acgtcaaccc gcagtccacc ctgctgctgg gccaggcgcg cggcgcactg atcactgcac
     1801 tggtcaccca gaacctgccg gtggccgaat acaccgccct gcaaatgaaa caggcggtcg
     1861 caggccacgg tcaggcgcag aaggcccaga tccaggaaat ggtgaagcgt ctgctggcgt
     1921 taccgggtct gcccggacca gacgcggccg atgcgctggg cttggccatt acccatgccc
     1981 atgcgggcaa agccatggcg cggctggcgg cggccgatgg cttgggcggc gtatcgaaaa
     2041 tgggggcgaa agcctcgtac cgcgcggggc gcagtcgcta aatcctggga gaaggctcag
     2101 gccttgtcat ccagcaacgc gcctgtcatg gcgttgctgg tcttgaaaac tgccagattg
     2161 gcgaggaagg cgtgcttggc ctgcaactgg gtcaccacat cggtttccag tgccgaacct
     2221 accgtcgcgg agcgtacggt ttgcgtggtc acgccaccac cggcctgttc accggccacc
     2281 acttcatgcc ggcgaaagcc tttagttggg aggttcgcca cgttgtgcgc cgacgcgttg
     2341 agttgcgtct gggccgcgtt catgccggag agcgcaatgc tggaaatgga agccatggtc
     2401 agtcccctcg ccaaagtggt gtctgggcgg attatgcaac ccgttccaaa ataagcacag
     2461 tgaagaagca aaacgccgcc agcagcgtcc atttcagcac cttccagccc cagtccttgt
     2521 agcgcggatt gccggtaccg acgtacagcg caaacgacac cgcggagcct agcaggcaca
     2581 acaggatcag gaatcggaac agcagcatgg ccgcaactgg ttaccaggcg cgcgcgggca
     2641 cggcaaaacc ggccggagcg agcttggcgt cctcaaaggt cacgatttcc caggcatcct
     2701 ggtgggccag tagttcccgc agcagtttgt tgttcagcgc atggcccgag cggaaagcgc
     2761 tgtaggcggc cagtagcggc ttgcccacga tgtacaggtc gcccatggcg tccaggatct
     2821 tgtgtttgac gaactcatcg tcgtagcgca agccgtcggc attgagcacc ttgtagtcgt
     2881 ccatcacgat ggcgttgtcc agaccgccgc cgagggccag tccgttggcg cgcatcatct
     2941 ccacgtcctt ggtgaaccca aaggtgcgtg ccctcgcgat gtcacgcgag taggagccgg
     3001 tgctcatgtc aaactccacc cgctggccgg taccgtccac cgcggggtgg ttgaagtcga
     3061 tctcgaaact cagcttgtag ccatggtacg gctccagacg tgcccacttc tcgttggcac
     3121 cgctgccttc gcgcacctcc accggtttga gcaggcggat aaagcgccgc ggcgcgttct
     3181 gcagctcaat gcccgcgctt tgcagcagga acacaaaaga cgcagccgag ccgtcgagaa
     3241 tcggaatctc ttcggcggtg atgtccacat acaggttgtc tacgcccaga ccggcacagg
     3301 ccgacatcag gtgttccacc gtattcaccc gcgcatcgcc gttggacagc gtggatgcca
     3361 ggcgggtatc cgtcaccgcc gtggcagaga caggaatgtc caccggctgc ggcaaatcca
     3421 cccgccgaaa cacgatgccc gtatccggtg cagccgggcg caaggtgatt tccacccgct
     3481 gcccgctgtg caggcccacg ccaacggcct gggtcaggga tttaagtgtt cgttgtttca
     3541 gcaccggtct attgtaaaag tagcgcactt gtccctccgg ggcgcgtgta tttcgccaga
     3601 aacaccgtgg aaccggctac gccgggccac tggtgttgcc ccctgcaagg gggaaagcgc
     3661 agcgacacgc agtgcgcgaa gccggggggt gtgccccatt tagtcagcct gtttgcgcag
     3721 gaaggccggg atctcgtaat catccatgcc gccgctggag agcgcgtcca ccttggcagc
     3781 ggcctgggtc cggttggtac gccacacgct gggtgtggac atgctgccgt agtcaggctg
     3841 cgtcaagcca ccgctggcgg cgccaccggc cgaagctgtg atgcctgcgg gctgggcgat
     3901 gttgttgagc gtgggcacat ggaacggaac gttgtcggta ccggtgcgca gcacctgcag
     3961 cggaggtgcc gtgcggcggg cgccctggcg ggacaagccg gtagccacca cggtcacgcg
     4021 gatctcgtca cccaatgcgt cgtcgtaggc ggtaccgtag atcacatgcg cgtcgggcga
     4081 ggcgtaggcg cggatggtat tcatcgccag cttggattcg ctcagtttca gggatccctt
     4141 ggccgcggtg atcagcacca gcacgccctt ggcgcccgag aggtcgatgc cttcgagcag
     4201 cgggcaggcc acggcctgct cggcggcaat gcgggcgcgg tccgggccgc tggccacggc
     4261 ggtgcccatc atggccttgc cgggctcccc catgacggtg cgcacgtctt caaagtcaac
     4321 gttcacgtgg ccgggcacat tgatgatttc ggcaatgccg ccgaccgcat ttttcagcac
     4381 gtcgttggcg tgggcaaacg cttcatcctg gctcacgtcg tcgcccagca cttcgagcag
     4441 cttctcgttg agcaccacga tcagggagtc cacattggct tcgagttcgg ccaggccggc
     4501 atcggcattg ctcatgcggc ggccgccttc gaagtcgaaa ggcttggtca ccacacctac
     4561 ggtcaggatg cccatctctt tggccacctt ggcaatgatg ggtgccgcac cggttcctgt
     4621 gccgccgccc atgccagcgg tgatgaacag catgtgcgcg ccttcaatcg cgttgcggat
     4681 atcggcctca gcctgctccg ccgcttcacg gcccttgtcg ggcttgctgc cagcgcccag
     4741 gccgctgccg ccgagctgga tggtcttgtg tgcggtactg cgcgagagag cctgcgcgtc
     4801 ggtgttcgcg cagatgaact ccacgccgcc cacggctgtg gcaatcatgt gttccaccgc
     4861 gttgccgccg ccgccgccca cgccgatgac cttgatctgg gtgccctggt tgaaagcttc
     4921 ttcttcaatc atttcgatgg ccattttttt ctcctgaggt ttcactaatt tcaataggtt
     4981 ggatgcttga ctgttgcagt actctgcgaa tgtgggaggt ggcccggtgc cacgccatcg
     5041 ttcatgcgga ctcccccgcg ccagccatag tctgtgtttc atggtcagaa gttccccaca
     5101 aaccagtcct tgacggagct gaaggctgtt ttgaccgagc cggctttctg ggccaccttg
     5161 tagccacgca gacgcgccag gcgggcctct tcgagcaggc ccatgacggt ggcggcacgg
     5221 ggctgcgcca ccatgtcggc cagggcgctg gaatacttgg gtacgccgcg gcgcaccggc
     5281 ttgaggaaga tgtcttcgcc cagttccacc atgcccggca tgatgcagct gccaccggtc
     5341 agcacgatgc cgctggagag cagttcctcg aagcccgatt cgcgcatgac ctggttgacc
     5401 agcgagaaga tttcttcaat gcgcggttcg atcacccctg ccagcgcctg gcggctgagc
     5461 atgcgcggac cgcggtcacc caggccaggc acttccacct gcgtctcggg gtccaccagc
     5521 agttgcttgg cgtggccgta ctcgaccttg atatcttcgg cgtccttggt aggcgtgcgc
     5581 agggccatgg caatgtcgct ggtgatcagg tcaccggcaa tcggaatcac ggccgtgtgg
     5641 cggatggcgc cgttggtgaa caccgctaca tcggtggtgc ccgcaccaat atcgaccagg
     5701 gccacaccga gttcgcgctc gtcttcggtc agtacgctca ggctgctggc cagcgggttc
     5761 agcatgagct gctccacctc caggccgcaa cggcgtacgc acttgatgat gttttcggcc
     5821 gcgctctgcg cgccggtgac gatatgcacc ttggcttcga gacggatgcc gctcatgccg
     5881 atgggttcct tgacgtcctg gccatcgatg atgaattcct gcggctcgac cagcagcagc
     5941 cgctggtcgg tcgagatgtt gatggccttg gcagtttcca ccacacgcgc cacgtcagct
     6001 gcggtgactt cacgatcctt gatcgccacc atgccgctgg agttgatgcc gcggatgtgg
     6061 ctgccggtga tgccggtgta cacgcgcgtg atcttgcagt cggccatcag ctcggcttct
     6121 ttcaaagcct gctggatgct ggccacggtg gcgtcgatgt tgaccaccac gccgcgcttc
     6181 aatccgttgc tgggcgcaat ccccaggcct gccagcttga gttccccccc aggcataacc
     6241 tcagccacca cggccatgac cttggcggtt cctatgtcta gccctacgac gaggtctttg
     6301 tactcttttg ccatgtgtgc acctgctcta cgtttctctg tctactgcta cttcttgggt
     6361 ccatcggccg cgacggtact cacaccgcgc agccggatgg cataaccgtt ttcatggcgc
     6421 aggtcggcga attccaccgc gctgggctgt cggccatagc gcgtaatcac ctgcgtcaat
     6481 gttttcaaaa accgctggac ccgagccgac acatcgccaa caccgccccg ccccagttcg
     6541 atcaccgccc cggtgtccag acgggcgcgc cagctgcccc ctgtggacag gtccagggcc
     6601 tcgatcggaa tatccatctg ctcaaacatg tcctgtagcg cctggtacat ggccagtacg
     6661 tcgcccgcct ggccgtccgg gccatgcagg cgcggcagcg cgtcctgctc tacttcgccc
     6721 acgttggcct caaagacctc gccatagctg ttgaccagac gctcatcgct ctcgccaccc
     6781 caataggcga ccggctggtg ctcctgcagg tccacgcgca ggcggttcgg aaagtcccgg
     6841 cgtaccaccg cacgacgcac ccagggcacc gactcgaatg cggcccgcac ccgcgccaga
     6901 tctaccgtga aaaaggtacc gctcagtcgg ggcgccacat tggcgcgcaa agtcaccgtg
     6961 ttgttgtggg cgacatcgcc actgaccaga atgccctgga tgtcaaacgc cggcaggcgc
     7021 gaaacccatc gcgccgcagc cactgcgccc agcacgacga aagccagtcc caaaaccatg
     7081 gcggtcatgt tcatcagctt cacgtcaagg ggagtggcta cagggttcat acagaagccg
     7141 aagccctcgc gtccagttcg gtgtgctgca gcagctgcac gcacaggtct tcgtagctga
     7201 tgccgtcggc gcgggctgac atgggtacca gcgaatgacc ggtcatgcct ggtgaggtat
     7261 tgatttccag caggtagggt ttgcgcgtgg cggcgtcgat catgacgtct gcacgggccc
     7321 agccgcggca gcccagtacc tggtaggcct ggcaaacgat ggcctggatg gcctcttcct
     7381 caccggctgg cagtccggcc ggcaccaggt actgcgtggt gtcagtgaag tacttgttct
     7441 ggtagtcgta gttaccgtca ggcgccacga tgcggatcac cggcagggcg cgcggcgatt
     7501 ccgtggcacc caagaccggg caggtaactt catcacccgc aatgaactgt tcgcacagca
     7561 cttcgttgtc gtgtttggca gctgctgcat aggccgcatc gcactggtcg gcggtcatga
     7621 ccttcgtgaa tccgatgctg gatccttcgc gcgcaggttt cacgatcatg ggcgcaccca
     7681 gctcggcaaa ggccgcatgc gtttgcgccg cactgctcac ctgtttccag gccggtgtgg
     7741 gcagaccttc aaagcgccag atgcgcttgg tcatgacctt gtccatggag atggccgacg
     7801 ccatcacacc cgaaccggtg tagggaatgc ccagcaattc gagtgcgccc tgcaccgtgc
     7861 cgtcttcgcc aaagcgtccg tgtagcgcga taaagcagcg gtcaaaccct tcacgcttga
     7921 ggtcatcgag cggacgctcc gccggatcaa atgcatgggc gttgacgcca cgcgccagca
     7981 gcgccttgag gacgccgcca cctgacatca gggacacttc gcgctcggcg gacgctccac
     8041 ccatcagcac cgccaccttg cccaggctgg cagcaatttt gttggcaatt cggctcatag
     8101 cccccgccct ttctgcgcag cgtgctctgt attttgtagc agattcacaa tttttccagg
     8161 caccgcaccg atcgatccag ctcccatgca cagcagcacg tctccatcct gggcgttgtc
     8221 gacagcggcc tgtggcatgg ccgcaatgtc gtccacaaag atcggctcta ccttgcccgc
     8281 cacccgcagg gcacgtgcca gtgagcgacc gtccgcagcc acgatgggtg cttcgccagc
     8341 ggcgtacacc tcgcccagca gcacggcatc agcctgtccg atcaccttga cgaagtcttc
     8401 aaagcagtcg cgggtgcgtg tgtaacggtg gggctggaat gccagcacca gacggcgccc
     8461 cgggaaggcg ccgcgcgccg ctgccagggt ggcggccatt tccaccgggt ggtggccgta
     8521 gtcgtcgatc acggtgaccg tgccgccgga gggtaaaggc aaatcgccgt aacgctggaa
     8581 gcggcggccc acacccttga attcggccag cgccttgacc acggcggcat cagcaatatt
     8641 gagctccacg gccaccgcaa tcgctgacag cgcattgagc acgttgtgca ggcccggcag
     8701 gttcagcacc acgtccaggt cgggcagcac cacgccgttg cggcgctgca ccgtgaagtg
     8761 catctgtccg tccacagcgc gtacgttgat ggcgcgcacc tgcgcttcct cgttgaagcc
     8821 gtagctggtg atcgggcagg tcacctgctc acagatttcg cgcacggcag cgtcatcggt
     8881 gcacaggatg gcggtgccat agaagggcat gcggtgcagg aagtcgacga acgccttctt
     8941 gaggcggccg aagtcatgcc cgtaggtttc catgtggtcg gcgtcgatgt tggtcacgac
     9001 cgccatcacg ggtagcagat tcaggaagga cgcatcggac tcgtcggctt cgaccacgat
     9061 atggtcgccg ctgcccaggc gcgcgttggc acccgcgctg ttgaggcgcc cgccaatcac
     9121 aaaggtcggg tccagaccgg cttcggccag cacactggcc accaagctgg tagtggtggt
     9181 cttgccgtgg gtgcccgcaa ttgcaatgcc ctgcttgaga cgcatcagct cggccaacat
     9241 cagggcgcgc ggcacgatgg ggatgtgcat ttcgcgagcg cgcacgactt cggggttgtc
     9301 ggattgcacc gcagtggagg tcaccaccgc atcggcacct gcgacgtggg cctcagcgtg
     9361 gcccacatag gtggtgatac ccagggacgc caggcgtttg agggtggcgc tgtcggaaag
     9421 gtccgagccg gagatgacat agcccaggtt gctgagcact tcagcgatgc cggacatgcc
     9481 ggagccgccg ataccgacga agtgaatatg tttgatggcg tgtttcatgg cgcgagttcc
     9541 tcgcaggccg ccaccaccgc atcggtggcg tgcagctgtt gcatgttttt ggccgccagc
     9601 ccgcgttgca aaagcgcaga acgctctgtt ttttgtagca aatcagccag cacctggggg
     9661 gtcatgtccg attggggcag cagccagccg gcgccctggt cgaccaggaa gcgggcgttg
     9721 gaggtctggt ggtcgtccac agcagacggg aaagggacaa agacggcagc ggcacccacc
     9781 gcggcaatct cggtcacggt gctggcaccg gcgcggcaga ttaccaggtc agcatcggca
     9841 aaggcgtggg ctgtgttgtc gatgaacggg gtcagcgtgg cctgcacgcc ggcggcggcg
     9901 tagttggctc gcaattcgtc gatctgtttt tcaccggcct ggtgcgtgac catcgggcgt
     9961 tgcgcctcgg gaatcaacgc cagggcttgc ggtaccacgg tattgagcgc gcgcgcgccc
    10021 aggctgccac cgaccaccag caccttgagc gggccactgc gaccggcaaa gcgctcctgc
    10081 ggtgcgagct gggtcaggaa ttccgcgcgc aggggattgc ctacccactg tcctttggtg
    10141 aacaccttgg ggaacgcggt gaatacgcga tcagccacac ccgccagtac cttgttggcc
    10201 atgccagcca ccgagttctg ctcatgcagc accaacggct tacccagcag caccgacatc
    10261 agcccgccgg ggaaggtgat gtagcccccc agacccacca ccacttgcgg gcgcacccgg
    10321 cgcaccacct ggaggctttg ccagaaggcc ttgagcaggc gcaacggcag cagaaccagc
    10381 gtgcggaggc ccttgccacg tacgccggca aaatccaccg tttcgagcgg aaagccgcgc
    10441 gccggcacca gccggctttc catactggct ggggcaccga gccagtgcac gcgccagccc
    10501 ttgtcacgca gcgcctgcgc caccgccagc cccgggaaga tatgtccgcc ggtgccgccg
    10561 gccatgatca atgcacaagg ttgggtactc atgcgcgccc cccatgcatc atttgccggt
    10621 tctcgaaatc gatgcgcagc accaccgcga tggcaatcat gttgagcagg atggccgagc
    10681 cgccgtaact catcaggggc agggtcagcc ccttggtggg caaggccccc agattcacgc
    10741 ccatgttgat gaaggactga aagcccatcc agatgccaac accctgcgcc accagaccgg
    10801 cgaatacgcg gtccagcgca atggcctggc ggccgatatg catgatgcga cgcgtcagcc
    10861 acaggaaaag gccaatcacc gtcagcacgc cgatcagtcc aaactcttca ccaatgaccg
    10921 ccagcaggaa gtcggtgtgt gcctcgggca accagtgcaa cttctccaca ctgccgccga
    10981 gtcccacgcc aaagatttca ccgcggccaa tggcgatcag cgaatgcgaa agctggtagc
    11041 ccttgcccag cgcgtatttc tcggtccagg ggtccaggta ggcaaagatg cgttcgcgcc
    11101 gccagtcgga cagggcaatc atcaggctga acgccaccac cagaatggcc gcaatcagga
    11161 agaacatgcg tgcattgacg ccacccagga acaggatacc catggcgatc acggcgatca
    11221 ccatgaaggc gcccatgtcc ggttcggcca gcagcagcag cccgacgatc gccaccgcca
    11281 ccgccatggg tgccaccgca cggaagaacc gttctttcac atccatcttg cgcaccatgt
    11341 agtccgcggc gtacagcagc accgcgaact tggccagctc ggacggctgg aaattcagaa
    11401 tacccagaga gatccagcgg cgtgcgccat tgacgccctt gccaatgaag ggcacaagca
    11461 ccgcaatcag cagtaccagc gatgccacaa aaagccaggg cgccaccttc tcccaggtct
    11521 gcaccggcac ctggaaagcg atcagggcac ctacaaacgc cacaccaatc gacaccacat
    11581 ggcgcaccag gaaatgggtg tgcgaatagt tggcaaagcg cgggttgtcc ggcatggcaa
    11641 tcgaagcgga atagaccatc accaggcccc acagcaacag ggtcaccacc acccagacca
    11701 gcggctggtc aaacccgtgc acgcgcgctg gcgtggtggt ggccgaaaac gaaccccgcc
    11761 ctcccagacg caccggcaag gccgcagctg ccgccggcgc catacgtgac gagaaccaac
    11821 ctgcaatggt ctggaacagg gcgctcatgc ggcgttctcc atatccacac cggcatccat
    11881 ggcgagttcc ttgacagcgg ctacaaagac acgcgcacgg tgctcgtagt tgtcaaacat
    11941 gtcgaaactg gcgcaggcgg gcgacatcag taccgcatcc cctgcatggg cacgctggtt
    12001 ggcctgcgct accgcctcgc tcatgctgcc agcatccgcc agcgcaaccc cggtgcgttc
    12061 cagtgcagca ctgatcagcg gagcatcgcg tccgatcagc accacagcac ggacgtaacg
    12121 cgccactggc gccgccagcg gcccaaagtc ctggcccttg ccttcgccac ccagaatcac
    12181 caccaccttg cggtcggcgc ccagtccctg cagggcggcg accgtggcgc ccacgttggt
    12241 gcccttgctg tcatcaaaga actccacgcc attgaggatg cccaccggct ccacacggtg
    12301 cggctcaccc cggtattcgc gcaggccgta gaggatgggg ccgagcgcac agcctgcagc
    12361 cgcgctcaat gccagggccg ccagcgcatt gctggcgttg tggcgccccc ggatacgcag
    12421 ggcgtccgca ggcatcaggc gctgaatgtg cagttcttct tcatcgtcct tgcgtttctt
    12481 gcgcgtttcg tctgcttcca gcgcacgcac cagccagacc atgccattga cgtcttcgag
    12541 cccatagtca ccggggcgct tgggcatgtc ggtcccaaac gtggtgtggg cccgctcctg
    12601 cgggcgttgc agcttgacgc gcaccggctc tggcagcatg gccatgactg cagcatcgtc
    12661 gcggttgagc accatcagtc cgtgctggcc aaaaatgcgc gtcttggcct gtgcatacgc
    12721 ctgcatgctg ccgtgccagt cgaggtgatc ctgtgtgaca ttgagcacca ctgcagcggt
    12781 gggctcaaag ttgctcacgc cgtcgagctg gaagctggac aactccagca cccagacttc
    12841 aggaagggta tcggcatcca ggtgactgga cagcgtatcg agcagggtcg ggccgatatt
    12901 gccggccacc gccacgggct tgcccccgtg ggccaccaac tgccaggtca gggaggtcac
    12961 ggtggtcttg ccattggttc cagtgatcgc caatacagag ggtgcgtaag cccgtgccgt
    13021 gcgcagggtc tgcaaggcca tggcaaacag actcagttcg ccacccacat aaatgccggc
    13081 ggctcgggcg gtatcgcata ccggagccac cacttccgga ctcaagcctg gagaacggta
    13141 caccgcatgc aggctctggc cttccaccaa cgcggcttgg aattcaccag agacgaattg
    13201 cacctggggc aactcctgct ggagcgtcac cagctgcggc ggtgcagcac gtgtgtcggc
    13261 cacgatcaca ctggcggcac cggtgcgcac gcaccaacgc gccatggcca ggcccgaagc
    13321 gccaagaccc aggatgagga tgttttgccc ggccagtggt tgcaagggag cgtccgcttg
    13381 tccatcgggt acagcgtcct gcacttcttc tggcacggac gggacatctt ccgccacttc
    13441 cacgggttcg gctttttccg acagggtttc agcaaaaatc tgtgccacga aagctgctgc
//
LOCUS       DAOSJM010000012        13469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4671_length_13469_cov_4.684881, whole genome shotgun sequence.
ACCESSION   DAOSJM010000012 DAOSJM010000000
VERSION     DAOSJM010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13469
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4671_length_13469_cov_4.684881"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1024)
                     /locus_tag="PKX82_00965"
     CDS             complement(128..1024)
                     /locus_tag="PKX82_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HOF50843.1"
                     /translation="MLDYASLSALAVVVREGSFERAARALHVTPSAISQRIRLLEERV
                     GCALVVRGQPCHATDMGRRLCQHVDRVHLLEQELKDALPALAPDGPVRVRLPIAVNAD
                     SLATWFSPAVAAFAADAPVLMDVTVDDQDHTSEWLRSGAVLAAVTGNARPATGCNSKP
                     LGAMRYLAAASPAFVARYFSDGVGAGSLARAPSLVFNTKDELQTRWAHRLCHRHVELP
                     RHTLPSSQAFVTAALAGMGWGLHPQALIAAHLEEGSLVTLLPDAPLDVPLYWQQARAA
                     SALLDGLNRQILSAAQAALIQA"
     gene            1098..1748
                     /locus_tag="PKX82_00970"
     CDS             1098..1748
                     /locus_tag="PKX82_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HOF50844.1"
                     /translation="MQTLSTTVSLIFPVVVQGLALSFGLIVAIGAQNAFVLRQGLRRE
                     HVGSVVLFCALADAVLIAAGVMGMAQALGERPDLARALALAGAAFLAYYGFAALQRAR
                     QAHRLQAADSGAGLSRAAAVAQAAAFTLLNPHVYLDTVLLVGSIGAQQPAPLRGWFVV
                     GASTASLLWFGLLGFGARWLAPWFARPRAWQVLDGLIALTMWVLAGLLVRHALNGM"
     gene            1758..2396
                     /locus_tag="PKX82_00975"
     CDS             1758..2396
                     /locus_tag="PKX82_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HOF50845.1"
                     /translation="MYVTKHHQLDDREAMFSLMDAYPLGAWVCAGQDGLVANHVPFLL
                     DRNQGPAGTLIGHVARANPVWRALHTATPSVVLFQGPQSYITPGWYPGKAEHGKVVPT
                     WNYMVAHAHGVARAVEDRDWLLDMLNRLTRVHEAMQPTPWRVADAPADYIDAMLRAIV
                     GIEIPIDRLEGKLKASQDEDMQDRIGTVRGLQQCPGEAARAMANLVHHTLPP"
     gene            2482..3498
                     /locus_tag="PKX82_00980"
     CDS             2482..3498
                     /locus_tag="PKX82_00980"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOF50846.1"
                     /translation="MTEPKPPLTLRRAPPPSKESAPTGVRRAPPPAARAPGNRAIPTG
                     QANTAAQGANGTVRLNKRMAELGMASRREADDWIAKGWVKVNGQVAEMGLQVAPDARI
                     EIDKAAKGQQANQVTILLNKPLGIVSGQAEDGHEPAIKLIQPQNRWAEDNARFFFHGS
                     QLKSLVPAGRLDIDSTGLLVLTQDGRVARQLIGEDSCVEKEYLVRVAYTGVANPGAAN
                     SAAATYPGLPGRSKPQQLIRLDDDDPITSDVQSVFPADKLQLLRHGLRLDDQPLKPAK
                     VEWQNPEQLRFVLTEGKKRQIRRMCELVGLKVVGLKRVRVGRVMLGNLPVGQWRYLQP
                     HEKF"
     gene            3516..4772
                     /locus_tag="PKX82_00985"
     CDS             3516..4772
                     /locus_tag="PKX82_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF50847.1"
                     /translation="MPKKPPSPLKALQHLRPSDLRGVAQLATQATRGVTRIAEGVHQS
                     VWSSMGVSGSAPEKTGGVTGFVYRTVHGVTELVGKGLQAGLTKLEPLLATVMDAPPET
                     PQREAVLAALNGVMGDRLAESYNPLATPMTLRYQGRALLWGSAPQLPGATNKVALLIH
                     GLCMNDLQWHSQADDGTPVDHGATLAALGYTPVYVRYNTGLHTSENGHLLAQQLEQMV
                     AHWPVPLQEITVVAHSMGGLITRSAVHSASQAPAPRWLSLLKSIVFLGTPHHGAPLER
                     AGNWVDTVLGSTPFTKPLSRLGKLRSAGITDLRYGHMVDADWQGHDRFRRKPDSRKPL
                     PLPEGIACFTVAATVAGERSALAERLIGDGLVPLDSALGKHDDPKRTLVFAKSSQCVM
                     YRLNHMELLSSPKVSQKLVEWLRVCA"
     gene            complement(4756..5004)
                     /locus_tag="PKX82_00990"
     CDS             complement(4756..5004)
                     /locus_tag="PKX82_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50848.1"
                     /translation="MAKKSETPKSPTPAAAGRVPDYLLDPVPVPDVVESDSDTAWGLW
                     QDSVQSLDVADTRAPGDSVFDRTIPSDLTPTLPVKRKP"
     gene            5196..7184
                     /gene="htpG"
                     /locus_tag="PKX82_00995"
     CDS             5196..7184
                     /gene="htpG"
                     /locus_tag="PKX82_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOF50849.1"
                     /translation="MSKQTLSFQAEVAQLLHLVTHSLYSNKEIFLRELISNASDACDK
                     LRFEAINDSGLYENDAELKVKVTFDKDAKTLTITDNGIGMSAQEAIDHLGTIAKSGTK
                     DFVSKLSGDQKADSQLIGQFGVGFYSGFIVADKITVESRRAGMQETEGVRWVSGGSGD
                     FEVETITRAERGTSVILHLREDALDYAQAWKVKGIINKYSDHISLPIQMEKEEWKDGE
                     LINPSDEKGGRQPGGMVKTGEWETVNKANAIWARAKKDITQEQYDDFYKQISHDFEAP
                     LAHTHNRVEGSTEYTQLLYIPGKAPMDLYNREKSAGVKLYVKRVFIMDDAEALLPTYL
                     RFVKGVVDSADLPLNVSRELLQESRDVKAIREGCTKRVLGMLEDLAKHDKLPTSDATT
                     EGVSDVLSAEEKAEAEAAAGKYSKFYAEFGAVLKEGLGEDFANKERLSRLLRFASSTT
                     DTVSVSLADYKARMKEGQEAIYYITADNAAAAKNSPQLEVFKKKGIEVLLMTDRVDEW
                     ALNYLHDFDGTPLQSVAKGAVDLGKLQDEAEKKAAEEAAEAFKPLLAKLKEALKDKAE
                     DVRVTTRLVDSPACLVVQDDGMSTQLARMLKQAGQQAPEVKPVLEVNAEHALVKKLDG
                     SVHFNDLAQILFDQALLAEGGMPADPAAYVKRVNALLV"
     gene            7503..8660
                     /locus_tag="PKX82_01000"
     CDS             7503..8660
                     /locus_tag="PKX82_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF50850.1"
                     /translation="MIESIYVTNFKSLGNVELKLGKFTCLIGMNGSGKSTVLQAIDFI
                     SQLMVGRVDEWLDARGWSVADLNCKLRKQSNVNVGVQFKTSTGKTLAWDGSFNRSQLR
                     CTGEIIQLDSERIFQVANQKYNIADRADQNIAFTYQGSLLSALKESELTDELLEFRNA
                     IQQIKSLELLSPQLLRKRARSTEKGIGAGGEKLSAYLDTLTDSTHTALVEQLRRFYPG
                     LVDFKVSSLRSGWKQLSVVEQFSQQKLETDAAHLNDGLLRILAVLTQAASERSLILLD
                     EIENGINQEIIETLVDTLVKSPHQLLVTTHSPLILNFLDDDVARKAVQFIYKAPLGET
                     RIRPFFDIPRINDKLRTMGPGDAFVDTDLRQLTEECLELDRHAGSFTIEDL"
     gene            8657..9391
                     /locus_tag="PKX82_01005"
     CDS             8657..9391
                     /locus_tag="PKX82_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50851.1"
                     /translation="MKILLSGEGPTDLGVCNNAQGRCDGADFKKGPMTQLLIQLLEPL
                     LGYSLADFPESFAYVSETALCAQTKATPARLQPTRGKKKGVETSYFYGNAMTLGRMAF
                     DLAAEVGDSVVAIFFRDTDGTHSSHTGLWQDKWQSVCDGFKHSDFTRGVPMLPKPKSE
                     AWLLCLAGFNPGGTCEALEELSGNDHSPNSVKSRLDATLGRHHSADELCEWLIQHPVA
                     VDRIDSMPSFRAFHEALISAVKNFPI"
     gene            complement(9608..10753)
                     /locus_tag="PKX82_01010"
     CDS             complement(9608..10753)
                     /locus_tag="PKX82_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HOF50852.1"
                     /translation="MPPLHISSQFDSGAIEVVSLDDPRNIELRIRRDTAAEFAQWFHF
                     CLHGANGRNVVLKFLNAGECAYPKGWEGYRVVASHDRQNWFRIDTEYDGHVMTAQTVP
                     QAQSIYFAYFEPYSHEQHLDLLALAGASPLVETQYLGATVQGRDMTVLRMTSAQAQPQ
                     DTSKKRIWIIARQHPGETMAEWFVEGCLERLLDVDDAVSRLLLERCELYVVPNMNPDG
                     SVLGNLRTNAAGANLNREWQEPSMDRSPEVFLVRQKMLETGVDLCLDVHGDEGLPHNF
                     VVGSEGVPAYTPRIAELEDAFKTAWQAVCPDFQDAVNYGRSEPGKANMALATNWVAQQ
                     FGCLALTVEMPFKDNANLPDPVTGWSAARSRKLGASVLTPILSVMAP"
     gene            10880..11269
                     /locus_tag="PKX82_01015"
     CDS             10880..11269
                     /locus_tag="PKX82_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HOF50853.1"
                     /translation="MTAFKRFIASTLIVATTLMGLPMTAQASIVSTETALQSTDSAAQ
                     RERVSTFLMRTDVQSALQSHGVTSESAVERVKAMSDDEVAQLAGRIDQAPAGGDILGI
                     LFTVFIILLITDILGLTKVFPFTRSMR"
     gene            11326..12222
                     /locus_tag="PKX82_01020"
     CDS             11326..12222
                     /locus_tag="PKX82_01020"
                     /inference="COORDINATES: protein motif:HMM:NF033920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA2778 family cysteine peptidase"
                     /protein_id="HOF50854.1"
                     /translation="MLWLTGCATPQVTSLQGQWPQDVPTQHQIAKVPFVAQDDFECGP
                     ASLAMAFGAAGVQVPLQTLVEQVYLPGRKGALQVEMLATTRRHGLLPYVLSQNLSAVL
                     REVAAGHPVIVFQNLSLPVYPVWHYAVVTGFDRDSSTLRLHSGKSENTEITFSTFERT
                     WARGEFWAMVALQPGDLPATAQPDPLVQSIAALERVNPAAARTAYTAALRQWPAHKGL
                     LLGQGNAAYALGQFSAAQQAYAQLVQAHPDFADGWNNLAQSLLDLNRREEARQAIDKA
                     IALGGPRLGQYRELQRRLSANP"
     gene            complement(12234..>13469)
                     /locus_tag="PKX82_01025"
     CDS             complement(12234..>13469)
                     /locus_tag="PKX82_01025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOF50855.1"
                     /translation="VLDSPEINAFALPGGYVYVTRGIMAYMESEADLAGVMGHEIGHV
                     TARHGAQRATREQNAGLGVFAASVLGAVAEAYGVVGAGQLAGQVSQTVAAGFIASYSR
                     DQELQADGLGAEYLSRTSYDPRNMVDVITVLKNQERFAADLAQAEGRPAPTKGDWLAS
                     HPSNDQRLQTITQLAAQYKGNYNDEGRARYLKVITGMGFGDSAEQGLTRGRNFYHVPL
                     GLALTAPVGWQIQNATDQLSVINAAGDAALFVKVVPPKAGTNHADIIRNVLKPTQGRT
                     EVTSINGLQATRFSGVRQNAQGKTQALEITLVTGPGTNTYLLQSSAKDAAALQRARAQ
                     LREAEGSFRALTEQDRGAARPWAIRTVPYPKGGFAELAKSSPIERPEQQLRLINGYYG
                     GGEPKVGQLVKVIASVGAP"
BASE COUNT         2699 a         4313 c         4069 g         2388 t
ORIGIN      
        1 taggttttta accctcaact cctgcgaaag cttgggttgt gcagaccttc cttagcgcag
       61 cggcataaag gaggagcccg aagggcgggg gacagccgcg gagctgagtg ccacgccgcc
      121 ctgcgcctca ggcctgtatc agtgcagcct gcgcagccga cagaatctgg cgattgagcc
      181 cgtcgagcag ggccgaagcc gcccgcgcct gctgccagta caaaggcacg tccagcggag
      241 cgtccggcaa cagcgtcacg agggaaccct cttccaggtg ggcggcaatc agcgcctgcg
      301 ggtgcagccc ccagcccatg ccggccaatg cggctgtcac aaatgcctgc gaggagggca
      361 gtgtgtggcg tggcagctcc acatgccggt ggcacaggcg atgcgcccag cgcgtctgca
      421 gttcgtcctt ggtgttgaac accagactgg gggcacgtgc cagactgccc gcgcccacac
      481 cgtcgctgaa atagcgcgcc acaaaagccg gactggccgc cgccaggtag cgcatggccc
      541 ccaggggttt gctgttgcag cccgtggcag ggcgtgcgtt gccggtcacg gcggccagca
      601 ccgcgccact gcgcaaccat tccgacgtgt ggtcctggtc gtccacggtc acgtccatca
      661 agacaggcgc gtcggcggca aatgcggcga ccgccggtga gaaccaggtg gccagactgt
      721 cggcattgac cgcaatgggc aggcgcaccc ggaccgggcc gtccggcgcg agcgctggca
      781 gcgcatcttt gagttcctgc tccagcaaat gcacgcggtc cacatgctgg caaaggcgtc
      841 ggcccatgtc ggtggcatgg cagggctgtc cgcgcacgac cagcgcacaa cccacccgct
      901 cctccagcag ccggatacgc tgtgaaatgg ccgacggcgt gacatggagc gcgcgcgccg
      961 cgcgctcaaa gcttccttcg cgaacgacga cggccagcgc agaaagggat gcatagtcca
     1021 acataaagta agtttttcta atttgtattt agaaaaggta gtttgtcttt attcttatga
     1081 aagggcaagc tctgcccatg caaaccttgt ccaccaccgt ttccctgatt tttcctgtcg
     1141 tcgtgcaggg cctggcgttg agcttcggcc tcatcgtggc cattggcgcg caaaacgcct
     1201 ttgtgctgcg gcagggcctg cgccgcgagc acgtgggcag cgtggtgctg ttctgtgcgc
     1261 tggccgacgc ggtgctgatt gcggcgggtg tcatgggcat ggcgcaggcc ctgggcgagc
     1321 gccccgatct ggctcgcgca ctggcactgg cgggcgccgc ctttctggcg tactacgggt
     1381 ttgcagccct gcagcgcgcc cggcaggcgc accggttgca ggccgccgac agtggcgcgg
     1441 gcctgagccg cgccgccgcg gtggcgcagg cagcggcctt tacgttgctc aacccccacg
     1501 tctacctgga cacggtgttg ctggtgggca gcatcggagc ccagcagcca gcgcccttgc
     1561 ggggctggtt tgtggtgggc gccagcacgg ccagcctgct gtggttcggg ctgctcgggt
     1621 ttggcgcacg ctggctggcg ccctggtttg cccgcccacg ggcatggcag gtgctggacg
     1681 ggctgattgc actgaccatg tgggtgctgg caggtctgct ggtgcgccat gcactcaacg
     1741 gcatgtaaag gacagccatg tacgtcacaa agcaccacca actcgatgac cgcgaggcca
     1801 tgttttcgct gatggacgcg tacccgctgg gggcatgggt ctgcgcaggc caggacggcc
     1861 tggttgccaa ccatgtgccg tttcttctgg accgcaacca gggacctgct ggcaccctga
     1921 tcggccatgt cgcgcgcgcc aacccggtct ggcgtgcatt gcacacggcc accccatcgg
     1981 tggtcctttt ccaggggcca cagtcctaca tcacacccgg ctggtacccc ggcaaggcag
     2041 aacatggcaa ggtcgtaccc acctggaact acatggtggc ccacgcccat ggcgttgcgc
     2101 gcgcggtgga ggatcgcgac tggctgctcg acatgctcaa ccggctcacc cgcgtgcacg
     2161 aagcaatgca acccacaccg tggcgcgtgg ccgatgcacc tgcagattac atcgacgcga
     2221 tgctgcgcgc catcgtcggc atcgagatac ccatcgaccg gctggaggga aagctcaagg
     2281 ccagccagga cgaggatatg caggaccgca tcggcacggt gcgcgggctg cagcagtgtc
     2341 cgggtgaggc ggccagggcc atggcaaacc tcgtccacca cacgctaccg ccctgatgcg
     2401 ccgcccgggg ctgagaaatt gctttaaatc tgccctcagc ccccgtaaaa tatgcgcaat
     2461 tagctacaaa aacaatagcg catgaccgaa cccaaaccac ccctcaccct gcgccgcgca
     2521 ccacccccaa gcaaggagag tgcaccgacg ggtgtgcgcc gtgcgccgcc gcccgccgcc
     2581 agggcaccag gtaaccgggc cattcccaca ggacaagcca ataccgccgc ccagggtgcc
     2641 aacggcaccg tgcgcctgaa caagcgcatg gccgagctcg gcatggcctc gcgccgcgag
     2701 gcggacgact ggattgccaa gggctgggtc aaggtgaacg gccaggtggc cgagatgggt
     2761 ctgcaggtgg cgcccgatgc gcgcatcgaa atcgacaagg ccgccaaagg ccagcaggcc
     2821 aaccaggtca ccatcctgct gaacaagccg ctgggcattg tgagcggcca ggccgaagat
     2881 ggccacgagc ccgccatcaa gctgatccag ccgcaaaacc gctgggccga agacaacgcc
     2941 cgctttttct tccacggcag ccagctcaag agcctggtgc ccgctggccg gctggacatt
     3001 gactccactg gcctgctggt gctgacgcag gacggccgcg tagcacgcca gctgattggc
     3061 gaggactctt gtgtggaaaa agaatacctg gtgcgcgtgg cctacaccgg tgtggccaac
     3121 cccggcgccg ccaactccgc cgccgccacg tacccaggat tgcccgggcg cagcaagccg
     3181 caacagctca tccgcctgga cgatgacgac ccgatcacca gtgatgtgca aagcgtgttt
     3241 cctgcggaca agctgcaact gctgcgccac ggcctgcgcc tggacgacca gcccctcaag
     3301 cccgccaaag tggagtggca aaaccccgag cagttgcgct ttgtgctgac cgaaggcaag
     3361 aagcgccaga ttcgccgcat gtgtgaactg gtcggcctga aggtggtggg cctcaaacgg
     3421 gtgcgcgtgg gccgggtcat gctgggcaac ctgcccgtag gccagtggcg ctacctgcag
     3481 ccccacgaaa agttctagac aaccgaagga tcggcatgcc caagaaacca cccagccccc
     3541 tgaaggcatt gcaacattta cggcccagcg acctgcgcgg cgtagcccag ctggccaccc
     3601 aggccacgcg gggcgtaacc cgcatcgcag aaggcgtgca ccagtcggtc tggagcagca
     3661 tgggggtttc cggcagcgcg ccagagaaaa ccggcggcgt taccggcttt gtctaccgca
     3721 cggtccacgg cgtcaccgaa ctggtgggca aagggctgca agcgggcctg accaagctgg
     3781 agcctttgct ggccaccgtc atggatgccc cgcccgaaac accgcagcgc gaagccgtgc
     3841 tggccgccct caatggcgtg atgggcgacc ggctggccga gagctacaac ccgctggcca
     3901 cgcccatgac cctgcgttac cagggccgtg ccctgctgtg gggcagcgcg ccgcaactgc
     3961 cgggcgcaac caacaaggtg gcgctgctga tccacggcct gtgcatgaac gacctgcaat
     4021 ggcacagcca ggctgacgac ggcaccccgg tcgaccacgg cgccacactg gcagccctgg
     4081 gctacacccc ggtgtacgtg cgctacaaca ccggcctgca cacctcggag aacgggcacc
     4141 tgctggcaca acagctggag cagatggtgg cgcactggcc ggtcccgctg caagagatta
     4201 ctgtcgtggc gcacagcatg ggcgggctga tcacgcgcag cgcggtgcac agcgccagcc
     4261 aggcacccgc accccgctgg ctgtccctgc tgaagagcat cgtctttttg ggaacgcccc
     4321 accacggagc tccgctggag cgggcgggca actgggtgga cacggtgctg ggcagcactc
     4381 cgttcaccaa accgctatcc cgcctgggca agctgcgcag cgccggtatc accgacctgc
     4441 gttacggcca catggtggac gccgactggc agggccatga ccgcttccgg cgcaagcccg
     4501 atagccgcaa gccgctgccg ctacccgaag gcattgcctg tttcaccgtc gccgccaccg
     4561 tggcgggcga acgcagtgca ctggcggagc gactgatcgg cgatgggctg gttccgctgg
     4621 acagcgcact gggcaaacac gacgatccca agcgcacact ggtctttgcc aaatcgtcgc
     4681 aatgcgtgat gtaccggctc aaccacatgg aactgctgag cagcccgaag gtgtcacaga
     4741 aactggtgga gtggctcagg gtttgcgctt gaccggcagc gtgggtgtga ggtcgctggg
     4801 aatggtgcgg tcaaagaccg agtcccccgg tgcgcgggtg tcagccacgt ccagagactg
     4861 gaccgagtcc tgccacaggc cccaggcagt gtccgaatca ctctccacga cgtccggcac
     4921 gggaacgggg tccagcaggt agtccggcac ccggccagca gccgcaggcg tcggcgattt
     4981 tggtgtttct gatttctttg ccatggatct tccttacccc caaaggtacc taattctggc
     5041 ctgttctgca agcggaaagc caacttagat cgctctgggg aaggtattcg cccttgaaac
     5101 ccacccccct cgcccataat tgagggttgc ctgcgcgacg caccaaaacg tcgcgcagcc
     5161 attccctccc agttgacgag aaccattgtt accttatgag caagcaaacc ctttctttcc
     5221 aggccgaagt ggcccagctg ttgcacctgg tcacccactc gctgtattcc aacaaggaaa
     5281 ttttcctgcg cgaactgatc tccaacgcgt cggatgcctg cgacaaactg cgttttgagg
     5341 ccatcaacga cagcggcctg tacgaaaacg acgcagaact caaggtcaag gtcacctttg
     5401 acaaggacgc caaaacgctc accattaccg acaacggcat cggcatgagc gcgcaggaag
     5461 ccatcgacca cctgggcacc atcgccaaga gcggcaccaa ggactttgtg agcaagctgt
     5521 ccggcgacca gaaggctgac tcccagctca tcggccagtt cggcgtgggt ttttactctg
     5581 gcttcatcgt ggccgacaag attaccgtgg aatcgcgccg tgccggcatg caggagaccg
     5641 aaggcgtgcg ctgggtcagc ggtggctccg gcgacttcga ggtcgaaacc atcacccgcg
     5701 ccgaacgcgg caccagcgtg atcctgcacc tgcgcgaaga cgcactggac tacgcccagg
     5761 cctggaaggt caagggcatc atcaacaaat actccgacca catcagcctg cccatccaga
     5821 tggaaaagga agagtggaaa gacggcgagc tgatcaaccc gagcgacgaa aaaggcggcc
     5881 gccaacccgg cggcatggtc aagaccggcg agtgggagac ggtgaacaag gccaacgcca
     5941 tctgggcgcg cgccaagaaa gacatcaccc aggaacagta cgacgacttc tacaagcaga
     6001 tcagccacga ctttgaggcg ccgctggcac acacgcacaa ccgcgtcgaa ggcagcaccg
     6061 aatacaccca gctgctgtac atccccggca aggcgccgat ggacctgtac aaccgcgaaa
     6121 agtccgccgg cgtcaagctc tacgtcaagc gcgtgttcat catggacgac gccgaggccc
     6181 tgctacccac ctacctgcgc tttgtgaagg gcgtggtgga ctctgccgac ctgccactca
     6241 acgtgagccg cgaactgctg caggaaagcc gcgacgtcaa ggccatccgc gaaggctgca
     6301 ccaagcgcgt gctgggcatg ctggaagacc tggccaagca tgacaagctg cccacgtccg
     6361 atgcgactac cgaaggggtt tctgacgttc ttagcgctga ggaaaaggcc gaagccgagg
     6421 ctgccgcagg caaatacagc aagttctacg cagaattcgg cgccgtgctc aaggagggcc
     6481 tgggcgaaga ctttgccaac aaggaacgcc tgtccaggct gctgcgtttt gcctccagca
     6541 ccaccgacac cgtgagcgtg tccctggccg actacaaggc gcgcatgaaa gaaggccagg
     6601 aggccatcta ctacatcacc gccgacaacg cggccgccgc caagaacagc ccgcagctgg
     6661 aagtgttcaa gaaaaagggc attgaagtgc tcctgatgac cgaccgcgtg gacgagtggg
     6721 cgctgaacta cctgcacgac ttcgacggca ccccgctgca atccgtagcc aaaggcgcgg
     6781 tcgatctggg caagctgcag gacgaggccg agaagaaagc cgccgaagag gctgccgaag
     6841 ccttcaagcc gttgctggcc aagctgaaag aagcgctgaa agacaaggcc gaagacgtgc
     6901 gcgtgaccac ccgcctggtg gacagccccg cctgcctggt ggtacaggac gacggcatga
     6961 gcacccagtt ggcccgcatg ctcaaacagg ccggtcaaca agcacccgaa gtcaaacccg
     7021 tgctggaagt caacgccgag cacgcgctgg tgaaaaagct cgacggcagc gtgcacttca
     7081 acgacctggc ccaaatcctg tttgaccagg cgctgctggc cgaaggtggc atgcctgcgg
     7141 acccggcggc gtatgtgaag cgggtgaacg ccctgctggt gtgatgacaa gggggattag
     7201 tggtcgaatc gacctctagc ccccgtccaa tatgcgcaag cagctactga atttgtagca
     7261 aacaatcgaa acccaaagcc ccgccagtct cacaactggc ggggcttttg attgaggttt
     7321 cggaacacta tttactctct gtacagtaca ttttgcaaat gcatcgcggc gcagtgccct
     7381 actgctgaca gattcagtga ccaacggaag cgttgcatga actaatgttt gtatagactg
     7441 cctttctcga taaggttgtc agctcgcgat aatcaaacct cagctcaatg ctggagaaaa
     7501 caatgatcga atcaatctac gtaaccaatt tcaagtcact ggggaacgtt gagctgaaac
     7561 tgggcaagtt cacctgtctg atcgggatga acggctccgg caaaagcacc gtgcttcagg
     7621 cgatcgattt catttcgcag ttaatggtcg gtcgggtaga cgagtggttg gatgcgcgtg
     7681 gttggagcgt ggctgatttg aattgcaagc tgcgcaaaca aagcaacgtc aatgtcggtg
     7741 tgcagttcaa gacttcaacg ggaaaaacac tcgcctggga tgggtccttc aaccggagtc
     7801 agttgcgctg cacgggagag atcattcagc ttgatagtga aaggatcttc caggttgcca
     7861 accagaagta caacattgcc gacagggctg atcagaatat tgctttcacc taccagggtt
     7921 cgctcctgtc agcactgaag gaatccgagc taacagatga gttgttggaa ttccgcaatg
     7981 caatccaaca aataaaatcc ttggaactac tgtcacccca gctgctacgc aagcgtgcac
     8041 gatccacaga aaaaggcatt ggcgcgggtg gcgaaaagct atccgcttac ttagacacct
     8101 tgacggactc aacacacacc gccttggtcg aacagcttcg tcgcttttac cccggtctgg
     8161 tcgatttcaa ggtgtcgtcc ttgcgctcgg gttggaagca gctttcggtg gtggagcagt
     8221 tctcacagca gaagctggaa acagatgccg cacatctaaa cgacggcttg ttgcgcatat
     8281 tggccgtttt gacccaagcc gcgtcggagc gttctttgat attgctggac gaaattgaaa
     8341 acggcatcaa ccaggaaatc attgagacct tggtggacac cttggtgaag tcaccacatc
     8401 aactactggt gaccacgcac agtccgttaa tcctgaattt tctggatgat gatgtggccc
     8461 gcaaggccgt tcaattcatc tacaaagcgc cgttgggtga aacgcgcatt cgcccgttct
     8521 tcgatattcc tcgcatcaat gacaaattgc gcaccatggg gcctggcgac gcctttgtgg
     8581 acaccgacct gagacaactc accgaagaat gcctggaact tgatcgccac gcgggcagct
     8641 tcaccattga ggacctatga agatactgct cagcggcgag gggccaactg acttgggcgt
     8701 gtgcaacaac gcgcagggcc gctgcgatgg agcggacttc aaaaagggcc ctatgaccca
     8761 gttgctgatc cagcttttgg agcccttgct tggatattcc cttgccgatt tcccagagag
     8821 ctttgcatac gtcagtgaaa ccgctttgtg cgcgcaaacc aaggcgacac ccgcacgcct
     8881 gcagccaacc cgaggaaaaa agaagggcgt agaaaccagc tatttttatg gcaatgccat
     8941 gacgctgggg cgcatggcct tcgatcttgc ggcggaggtg ggcgactctg tcgtagcgat
     9001 cttcttccgt gacaccgatg gcacgcatag tagccataca ggtctctggc aagacaagtg
     9061 gcaatcggtc tgcgacggat tcaagcattc tgatttcacg cgaggggtcc ccatgttgcc
     9121 caaacccaag tccgaagctt ggcttttatg ccttgcagga ttcaaccctg gcggcacctg
     9181 cgaggcgttg gaagaacttt caggcaacga tcactcaccc aattcggtca aatctcgctt
     9241 ggatgcaaca ctggggcgcc atcactccgc tgacgaattg tgtgagtggc tcattcagca
     9301 ccctgtggca gtagatcgca tcgactccat gcccagcttc cgtgcatttc acgaagcatt
     9361 gatttcagct gtgaagaatt ttccgatttg atggcttgca ggtgcgggag tgccggaggc
     9421 ggcatgccta cggacccggc ggcgtacgtc aagcgcgtga acgccctgct ggtgtgatga
     9481 caagggggat tagtggtcga atcgacctct agcccccgtc caatatgcgc aagcagctac
     9541 tgaagttata gcatcttgtc gaaacctaaa gccccgccag tctcacaact ggcggggctt
     9601 ttgcgtacta gggcgccatc acagacagaa tgggcgtcaa cacgctggcg cccagtttcc
     9661 gggaccgcgc cgcgctccag ccggtgacag ggtcgggcag gttggcgttg tccttgaaag
     9721 gcatttccac cgtcaatgcc aggcagccaa actgctgcgc cacccagttg gtcgccagcg
     9781 ccatgttggc cttgccaggt tcactgcggc cgtagttcac cgcgtcctga aaatccgggc
     9841 agaccgcctg ccacgcggtc ttgaaagcat cctccagctc ggcaatgcgc ggcgtgtagg
     9901 ccggcacgcc ttccgacccc accacaaagt tgtgtggcag gccttcatct ccgtgcacat
     9961 ccagacacag gtccacgccg gtttccagca tcttctggcg caccagaaac acctcgggcg
    10021 accggtccat gctgggctcc tgccactcac ggttgaggtt ggcgccagcc gcattggtgc
    10081 gcagattgcc gagcaccgaa ccatccgggt tcatgttggg caccacatac aactcgcagc
    10141 gttccagcag gaggcggctg accgcgtcgt ccacatccag cagccgctcc aggcaccctt
    10201 ccacaaacca ctccgccatg gtttctccgg ggtgctggcg cgcgatgatc cagatccgtt
    10261 ttttgctggt gtcctgcggc tgcgcttgtg cagaggtcat gcgcaacacg gtcatgtcac
    10321 gcccttggac cgtggcgccc aggtattgag tctccaccag cggtgaggcg cccgccaaag
    10381 ccagcaggtc caggtgctgc tcatgcgaat acggctcgaa ataggcgaag tagatgctct
    10441 gcgcctgcgg cacggtctgc gccgtcatga cgtggccgtc gtattcggta tcaatgcgga
    10501 accagttctg ccggtcatgg ctggccacca cgcggtagcc ctcccacccc ttcgggtagg
    10561 cgcactcccc ggcattgagg aacttgagca ccacattgcg gccgttggca ccatgcaggc
    10621 agaagtggaa ccactgcgca aactcggcgg cggtgtccct gcggatacgc agttcgatat
    10681 tgcgggggtc gtccaggctg acaacctcga tggcacccga atcaaactgg ctggaaatat
    10741 gaagtggtgg cacgcgaatc ccttttgtaa tacccttgct tcggctcagc cgggtttatg
    10801 tcacaagttg taaccaaact tcgggatgag cgcaatcctc ttctacactt cgcccattcc
    10861 cagtttcccg agagaaccca tgaccgcatt caaacgcttt atcgcttcca ccctgatcgt
    10921 tgccaccacg ctgatgggcc tgcccatgac cgcacaggcc tcgatcgttt ccaccgagac
    10981 cgccctgcag tccaccgaca gcgcggccca gcgcgaacgc gtgagcacgt tcctgatgcg
    11041 cacggacgtg caaagcgccc tgcaaagcca tggcgtgacg tctgagtccg cagtggagcg
    11101 cgtcaaagcc atgtccgatg acgaagtggc ccaactggcc ggccgcatcg accaggcacc
    11161 cgctggcggc gacattctgg gcatcctgtt caccgtgttc atcatcctgc tgattaccga
    11221 catcctcggg ctgaccaaag tgttcccgtt cacccgctcc atgcgatgaa cctgcggcaa
    11281 cgctgcatta cctcccccgc actggcgggg gtttttattt gtgccgtgct ctggctcaca
    11341 ggctgcgcca cgccccaggt cacgtcactg caaggccagt ggccgcagga cgtgcctacg
    11401 cagcaccaga ttgccaaggt gccgtttgtg gcgcaggacg attttgagtg tggcccggca
    11461 tcgctggcca tggcgttcgg agcagcgggc gtgcaggtgc cgttgcaaac cctggtggaa
    11521 caggtgtacc tgcccgggcg caagggcgcg ctgcaggtgg aaatgctggc cactacccgg
    11581 cgccacggtc tgctgcccta tgtgctgtcc caaaacctgt ccgcagtgct gcgggaggtt
    11641 gccgccgggc acccggtcat cgtgttccag aacctctcgc tgccggtcta tccggtgtgg
    11701 cactacgctg tggtgactgg ttttgaccgc gacagcagca ccctgcggct gcactccgga
    11761 aaatccgaaa acaccgagat cactttcagc acctttgagc gcacctgggc gcgtggcgag
    11821 ttctgggcca tggtggccct gcagcctggc gatctgcccg ccaccgcaca acccgacccg
    11881 ctggtgcagt ccattgccgc gctggagcgg gtgaaccccg ccgcggcgcg taccgcctac
    11941 accgccgcgc tgcgccagtg gccagcgcac aaagggctac tgttgggcca aggcaatgcg
    12001 gcctatgcgc tgggtcagtt cagcgccgcg cagcaggcct atgcgcagct ggtgcaggcc
    12061 catccggatt ttgcagacgg ctggaacaac ctggcccagt cgctgctgga cctgaaccgg
    12121 cgcgaagaag cccggcaggc gatcgacaag gccattgcac ttggcggccc caggctgggc
    12181 caataccgcg aactgcagcg gcgcctgtca gccaacccat aaaccgtcgc cggtcagggc
    12241 gcgcccacgc tggcgatgac cttgaccagc tgccccacct tgggctcacc cccgccgtag
    12301 tagccgttga tcagccgcag ctgctgctcc gggcgttcga tgggggacga cttggccagc
    12361 tcggcaaagc cgcccttggg gtacggcacg gtgcggatcg cccaggggcg cgcggcacca
    12421 cggtcctgct ctgtcagtgc ccggaaactg ccctcggcct cacgcaactg cgcgcgcgcg
    12481 cgctgcagtg ccgccgcgtc cttggcgctg ctttgcagca ggtaggtgtt cgtacccggc
    12541 ccggtgacca acgtgatttc cagtgcctgg gtttttccct gcgcgttctg gcgcacgccg
    12601 ctgaaccgcg tggcctgcag accgttgatg ctggtgactt ccgtgcggcc ctgcgtgggt
    12661 ttaagcacat tgcgaatgat gtccgcgtgg ttggtccccg ccttgggtgg caccactttc
    12721 acaaacaacg cggcatcccc cgcagcgttg atcacagaca gctggtcggt ggcgttctgg
    12781 atctgccagc cgactggcgc cgtcaacgcc aggcccagcg gcacgtggta gaaattgcgc
    12841 cctcgtgtca gcccctgctc tgcgctgtcg ccaaagccca tgcctgtaat caccttgagg
    12901 tagcgcgcac gcccctcgtc gttgtagttg cccttgtact gggcggccag ctgggtgatg
    12961 gtctgcagac gctggtcgtt cgaggggtgg gaagcaagcc agtccccctt cgttggcgcg
    13021 gggcggcctt cggcctgcgc cagatcggcg gcaaaccgct cctggttctt caacacggtg
    13081 atcacgtcta ccatgttgcg cgggtcatag ctggtgcgcg acaggtactc ggcccccagc
    13141 ccatcggcct gcagttcctg gtcgcggcta taggatgcaa taaaaccagc cgccaccgtc
    13201 tgcgacacct ggcccgccag ctgtccggcc cccactacac cgtaggcctc tgccaccgca
    13261 cccagcacac tggccgcaaa cacacccagc ccggcgtttt gctcgcgagt ggcacgctgc
    13321 gcaccgtggc gcgcggtgac gtggccaatc tcatggccca tcacaccggc cagatcggcc
    13381 tcgctctcca tgtaggccat gatgccgcgc gtcacgtata catagccacc gggcagcgca
    13441 aaggcgttga tctccgggct gtccagcac
//
LOCUS       DAOSJM010000013        13394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4705_length_13394_cov_4.796462, whole genome shotgun sequence.
ACCESSION   DAOSJM010000013 DAOSJM010000000
VERSION     DAOSJM010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13394
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4705_length_13394_cov_4.796462"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="PKX82_01030"
     CDS             <1..1090
                     /locus_tag="PKX82_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF50856.1"
                     /translation="EQAAATLCYTSGTTGNPKGVMYSHRAIVLNAISGCNPSILSLSP
                     ADAILPVVPMFHVNAWCIPYAAPIAGAKLVLPGPGLDGKSLYELMESEGVTVSAGVPT
                     IWQLLLAHVEQNGLKFTTMRRTLVGGSAMPRSLIAKFMDTYQVEVRHGWGMTETTAAA
                     TMGGLMPAAKGWPAEEKHAHLAKQGRTMFGVEIKVVDEQGATLPRDGESQGELMVRGQ
                     WVLSSYFKADRSPLVDGWFPTGDIATISPDGVMQIRDRTKDVIKTGGEWISSIDLESA
                     AVAHPAVAMAAVIGVKHPKWDERPLMFIVRKPGHALEKDEILKFLETQVAKWWVPDDV
                     VFLESLPVGGTGKVQKGDLRKQYGSVFS"
     gene            1135..3732
                     /gene="mutS"
                     /locus_tag="PKX82_01035"
     CDS             1135..3732
                     /gene="mutS"
                     /locus_tag="PKX82_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOF50857.1"
                     /translation="MTDTAPPHTPMMQQYLGLKADFPGTLLFYRMGDFYEMFYADAEK
                     AARLLDITLTTRGQSAGQPVVMAGVPFHSMEGYLAKLIRHGESVAICEQVGEVGASKG
                     PVERKVVRVVTPGTLTDSELLNEKSESMLMAVHQGPRNRCGLAWLSVTQGVVHLAECA
                     PDQVADWIGRVAPSELAYSAGCTTPFEDRLKRLRASSHALYLTARPEWQFDSALGQRK
                     LLDALQTASLSSWNAQDLPLAHAAAAALLGYAEHTQGRSLTHIHSVQVQRDDELMDLP
                     ATTRRNLELVQTLRGEDSPTLFSLLDTCMTGMGSRMLKSWLLEPRRDRSEAQHRLHAI
                     STLKSNLWQTLRGQLKGCADVERITARTALRQVRPRELVALVQTLQKTELLAQHLRAL
                     EGYLVHISQALQPPADCAVLLGRAIAAEPAALVRDGGVIADGFDAELDELRGIQTNCD
                     AFLLDLEVREKARTGIANLRVQFNKVHGFYIEVTTGQVDKVPDDYRRRQTLKNAERFI
                     TPELKAFEDKALSAQERALAREKWLFEQVLDQLQAHIPQLTRVARALAALDALCALTE
                     RALTLDWNAPQFVAEPCIDITQGRHPVVQARLAELSSGNFIANDTRLGPKARMQIITG
                     PNMGGKSTYMRQVALIVLLASMGSYVPASACRLGPIDAIHTRIGAADDLANAQSTFML
                     EMTEGAQILHSATPHSLVLMDEIGRGTSTFDGLALASAIATQLHDKTQAFTLFATHYF
                     ELTEFPATHHAALNVHVSAAEAGRDIVFLHAIEPGPASRSYGIQVARLAGMPAAVLNQ
                     ARHTLEALESHAADSQAQVDLFAAPPVTETATASAVETALVAINPDALTPREALEALY
                     QLKALTHKN"
     gene            3770..6238
                     /locus_tag="PKX82_01040"
     CDS             3770..6238
                     /locus_tag="PKX82_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011356107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HOF50858.1"
                     /translation="MTEHLQPGGLAHASNLDAIIIGTGFAGLGMAIALKQQGIENFVI
                     LEKTQDVGGVWRDNSYPGAACDVPSHLYSFSFEPNPDWSRVFATQGEIHSYLQHCARK
                     YGLLPHVRFGVEMRQAEFDERHSRWTVTTADGQQFTAKLLITATGQLSRPVLPQLPGL
                     ETFKGHTFHSAQWDHQYALDGKRVAVVGTGASAIQFVPAIADRVAQLSVFQRSPAYIL
                     PRPDRPYTGLEKKLFALPWLSKLHRAGIYLKYESRALAFTRFQWLMKPAGLMPFHRLL
                     KRQVQDPTLRAKMTPDYQIGCKRILLSSEYLATFAKPQVALCTEGIRRVTPEGIETQD
                     GKLHPADAIVFGTGFAATEFLAPMRITGRQGLDLQTAWKGGAKAYLGLTVPGFPNFFM
                     LYGPNTNLGHNSIVYMLESQIAHVVRCLKEMRATGTSTIEVREAPFLRFNAHIQQRLK
                     NSVWSVCTSWYVDAQGHNSTNWPGFTLSYRWLLKRGSLDAYRFSEPRTEAVAPTAEIA
                     APTDIVEQAQSTFLRGFLRVAFRTFIGPPYGAVAQRRWVNVLSILMPARSGVQRQTTV
                     IGNIPVTITHPKATTADGVVLYLHGGAFCVASPHTHRSITSHLAHASGMPVWAPDYRL
                     APEHPYPAALEDATSVYEFLLQQGYAPARIVLAGDSAGGALALALAIQLRDQDAPQPA
                     GLALISPVTDPTLGGASVQEQAGTDPMIRKGWVEQGVAWYGCPPGTPSHTPLATSFQE
                     LPPMHIQVGDREILLSDSVRLAEHAVACGVDCQLDIHRGRWHVFHLQALFLRSSVQAL
                     ETLAQFARARVHVALSTPPAKELP"
     gene            6235..7017
                     /locus_tag="PKX82_01045"
     CDS             6235..7017
                     /locus_tag="PKX82_01045"
                     /inference="COORDINATES: protein motif:HMM:NF006123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF50859.1"
                     /translation="MTQEARRCIFITGAANGIGRACARLFASQGWRVGVADIDQSALD
                     TLVQELGTEKAQAFRVDVTQAEDWAVALAAFHSQCGRLDVLLNNAGILISGPFAASPL
                     AQHHALVDVNIKGVLNGCYLAKQYLQATPDSRVINLSSTSAVYGQASLATYSSTKFAI
                     RGLIEALNIEWQDDGIRVMDIMPLYVRTAMLKDIKARSADRLGVQLTPEDVAATVWKA
                     ANFTGSFGKVHWPLNWQAVWMFRLTSMTPDWLARAIARRIAR"
     gene            7084..7911
                     /locus_tag="PKX82_01050"
     CDS             7084..7911
                     /locus_tag="PKX82_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HOF50860.1"
                     /translation="MTYCVAIKLNAGLVFLSDSRTNAGLDQISTFRKMIVYEKPDDRF
                     MVLLSAGNLSISQSVREILQIEKLKDHDDDEGITIWNAKSMFDAARVLGSAIRKVHDR
                     DAGSLKQAGVDFNVSLIFGGQIQGEGMRLFQVYSAGNFIEATPETPYFQVGESKYGKP
                     VLDRVITPTTPLDEAAKCALVSMDSTLKSNLSVGLPLDMVVYEANTFHTDKVVCIDEN
                     NPYFKMLHNSWGQKLREVFDSIEDPAWNGGRTDVPLLIPSTRSLPLKKITTPDEKLI"
     gene            7926..8729
                     /locus_tag="PKX82_01055"
     CDS             7926..8729
                     /locus_tag="PKX82_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF50861.1"
                     /translation="MPLIIFSHANSFPASTYKVLFKSLRSRGFTVKAPEKFGHDPRYP
                     VTNNWPHVVQQLADFALPEVDKAGEPAWLVGHSLGGFLSLMAAARFPELARGVVLIDS
                     PILGGWRSTAVGVMKSTQVMGSISPGAVSRQRRDSWPSVEAAYAHFRNKKAFAKWDDQ
                     VLLDYVTYGTSAIDGKRVLGFHRDVETLFYNTVPDNLDRLLSRHPVKCPVTFIGGRAS
                     HEMKQVGMAMTEKITKGRTMMLDGSHLFPMEKPLATAAAIEAALRNMES"
     gene            8738..10441
                     /locus_tag="PKX82_01060"
     CDS             8738..10441
                     /locus_tag="PKX82_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF50862.1"
                     /translation="MSEDSPTPLHLLEQKFQALQAEHDTLKQQKNEVFHQVFYRIAER
                     ATAGLSFYDFLKEVHTLMGELLPAINLYVALCNHDRDTVSFPYYVDERDGDIWQRDDT
                     PRKRNMTEYVLRTGTPQLIDAARFIALRDSGEITQAAGDLTFTSWLGVPLTLHGRVSG
                     VLAVQSYTEGVSYTDKDADVLAFVAHHVSSAIERYQAIDALRKSEERYRLVIEKISLG
                     VAVIQDGRMIFANPALVRILGYPLEWLLTQPFSAIAHPDDVPVMVERQRRRMRGEAVP
                     ADYTFRSITQTGEVHQLEVSAVLIEWGQRTATLAFVADATTRLHAEQAQRVALEQQTE
                     LNDIKTRFISMTSHEFRTPLASIHGSVELLMHYDDRLSVDKKRQILENIDDAVERMTH
                     MLGNVMQMGRSDAGQLQFRPKPLAFTPFCLSLIEELRETMPRQFAKVQLELDLLPTKH
                     QFMLDETLIRNIVGNLLSNAFKYSPRGGTVTFAARKKKGEIVLTVSDQGIGIPQADQA
                     LLFQTFHRASNVGAIAGTGLGLSIVKEAVDCHQGRIEVQSTEGEGSQFTVMLPASTVT
                     A"
     gene            10452..12200
                     /locus_tag="PKX82_01065"
     CDS             10452..12200
                     /locus_tag="PKX82_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF50863.1"
                     /translation="MHILIAEDEPSLRENLQWMLEMEGFTVVAASDGLQALEFACLAP
                     PSLVLTDVMMPHLDGYGLVKALRETMATSTVPIIMLTAKADRRDVRMGMNLGADDYLT
                     KPYRRDELLAAVRARLARKASLDETARRLQEEAREAQQRDALTALPGRELFGQRLAVT
                     LKQAPAENLHSIGLVCLGLDGFSKVNDSLGAAVGDKVLYEVACRLGEHMRDARDSLRY
                     DCVARLGSDLFAVYIGENTNLTALEESSAALLQVLAQPYLVDGHTLFLTACAGASLFP
                     MQAADAHGLLLNAETALHHAKPVGPGTVRVFDTAMNLQVARRLQVHNALHAALEQNEL
                     QVYYQPQVSIAAGNVVGFEALLRWHHPTLGSVSPAEFIPIAEESGLITRIGAWTMRTA
                     AAQAAQWHAQGHGTFRMAVNLSVRQFLGSDLPGLVRTVLEETGLPPHMLELEVTESLA
                     LHSVSSTLATLHACKALGVMLAMDDFGTGYSSLAYLKRYPLDCLKIDQTFVRNITQDI
                     GDAAITRAIVAMAHSFGMSVIAEGVETIEQLDFLRGLGCEEFQGFLFSKPVPAEEAVK
                     CFAGFMPPSAPLWSRP"
     gene            complement(12163..12996)
                     /locus_tag="PKX82_01070"
     CDS             complement(12163..12996)
                     /locus_tag="PKX82_01070"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOF50864.1"
                     /translation="MDFSLLIKAAIMGVVEGLTEFLPISSTGHLILAGALLGFDDDKA
                     KVFDIAIQTGAIFAVILVYWQKIRDTLIALPSQKQAQQFALNVLIAFVPAVVLGLLLG
                     KAIKAHLFTPTIVATTFIVGGFIILWAERRQAAGGNAVRIHSVDEMSTLDALKVGLVQ
                     CLAMVPGTSRSGATIIGGMLLGLSRKAATDFSFYLAIPTLIGAGVYSLYKERALLSVA
                     DVPMFAVGLVFSFASAWLCIRWLLRYIATHSFTGFAYYRIAFGIVVLVTAWTKVVHWA
                     A"
     gene            complement(13064..>13394)
                     /locus_tag="PKX82_01075"
     CDS             complement(13064..>13394)
                     /locus_tag="PKX82_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOF50865.1"
                     /translation="GIMNIMLVSVTERTREIGLRLAVGARGKDILAQFLIEAVTLSLI
                     GGAIGVGLGAVATWAVGAFAGWNVSLSLGAVLLAVGFSGVVGIFFGYYPARRASKLLP
                     IQALRYE"
BASE COUNT         2601 a         4480 c         4008 g         2305 t
ORIGIN      
        1 cgaacaggcc gctgccacgc tgtgctacac ctcgggcacc accggcaacc ccaaaggcgt
       61 gatgtactcc caccgggcca tcgtgctcaa cgccatctcg ggctgcaacc cgtccatcct
      121 gtcgctctcg ccagccgacg ccattcttcc cgtggtgccg atgttccacg tcaacgcctg
      181 gtgcattccc tatgctgcac ccattgcagg cgccaagctg gtgctgcccg gcccgggact
      241 ggatggcaag tccctgtatg aactgatgga gtccgaaggc gtcaccgtga gtgctggcgt
      301 tcccaccatc tggcaactgc tgctggccca cgtcgagcag aacggactga agttcaccac
      361 catgcgccgc accctggtgg gtggttcggc catgccacgc tcgctgatcg ccaagttcat
      421 ggatacctac caggtcgaag tgcgccacgg ctggggcatg accgaaacca cggccgcagc
      481 caccatgggc ggtctgatgc ctgcggcaaa gggctggccc gctgaagaaa aacatgccca
      541 cctggccaaa caggggcgga ccatgtttgg cgtggagatc aaggtggtgg acgaacaggg
      601 cgccaccctg ccccgtgacg gtgaatccca gggtgagctg atggtgcgcg ggcagtgggt
      661 gctgtcttcc tacttcaagg ccgaccgctc gccattggtg gacggctggt tccccacggg
      721 cgacattgcc accatcagcc ccgacggggt gatgcagatc cgcgaccgca ccaaggacgt
      781 gatcaagacc gggggtgaat ggatcagttc catcgacctg gagagcgccg ccgtcgccca
      841 cccggccgtg gccatggcag cggtgattgg cgtgaagcac cccaagtggg acgaacgccc
      901 cctgatgttc atcgtgcgca aacccggcca cgcactggaa aaggacgaaa tcctgaagtt
      961 tctggaaacc caggtcgcca agtggtgggt acccgacgac gtggtgtttc tggagtccct
     1021 gccggtgggg ggtaccggca aggtgcaaaa gggcgacttg cgcaagcagt acggcagcgt
     1081 tttcagctga gccgagccgg cagaatggcg ggctgagttg atagactcag cgggatgacc
     1141 gacaccgcgc caccgcacac ccccatgatg cagcagtacc tgggtctgaa agcagacttt
     1201 ccgggcacgc tgctcttcta ccgcatgggg gatttttatg agatgttcta tgcggacgcg
     1261 gagaaggctg cgcgcctgct cgacatcaca ctcaccaccc gcggccagtc ggccggacaa
     1321 cccgtggtca tggccggtgt gccgttccac tcgatggaag ggtacctcgc caagctgatc
     1381 cgccacggcg agtcggtggc catctgcgag caggtgggcg aagtgggcgc gtccaagggc
     1441 ccggtggaac gcaaggtggt gcgcgtggtc acgcccggca ccctgaccga ctccgaactg
     1501 ctgaacgaaa aatctgaatc catgctgatg gcggtgcacc aggggccgcg caaccgctgc
     1561 ggcctggcct ggctcagcgt cacccagggc gtggtgcatc tggccgaatg tgcgccggac
     1621 caggtggccg actggattgg ccgcgtggcc ccgagcgaac tggcctacag cgccggctgc
     1681 accaccccct ttgaagaccg gctcaagcgc ctgcgcgcca gcagccacgc gctgtacctg
     1741 acggcgcgcc ccgaatggca gtttgacagt gccctgggcc agcgcaaact gctcgacgca
     1801 ctgcagaccg cgagtctttc cagctggaac gcgcaggacc tgcccctggc ccatgccgcc
     1861 gcagccgctc tgctgggcta cgcggagcac acccagggcc gcagcctcac gcacatccac
     1921 agcgtgcagg tgcagcgcga cgacgagctg atggatctgc ctgccacgac ccgacgcaac
     1981 ctggaactgg tgcagacgct gcgcggcgaa gacagcccca cgctgttcag cctgctcgac
     2041 acctgcatga ccggcatggg cagccgcatg ctcaaaagct ggctgctgga gccccgccgg
     2101 gatcgcagcg aagcgcaaca ccggctgcac gccatcagta cgttgaaatc caacctgtgg
     2161 caaacgctgc gcggccagct caagggttgc gccgatgtgg agcgcatcac cgcccgcacc
     2221 gcgctgcgcc aggtgcgccc gcgcgaactc gtggccctgg tgcaaacgct acaaaaaaca
     2281 gagctactcg cgcagcatct acgcgcgcta gagggctatt tggtccatat ttcccaggcg
     2341 ctgcagcccc cagcggactg tgcggtgctg ttgggccgcg ccatcgctgc cgaacccgca
     2401 gccctggtgc gcgatggcgg cgtgattgcc gatggctttg acgccgagct ggacgagctc
     2461 cgcggcatcc agaccaactg cgatgcgttt ctgctggacc tggaagtgcg cgagaaagcc
     2521 cgcaccggca ttgccaatct gcgcgtgcag ttcaacaagg tgcatggctt ctacatcgag
     2581 gtgactaccg gccaggtgga caaggtgccc gacgactacc gccgccgcca gaccctgaaa
     2641 aacgccgaac gttttatcac ccccgaactc aaggcctttg aagacaaggc cctgagcgcg
     2701 caggagcgtg cactggcgcg cgagaagtgg ctgttcgaac aggtgctgga ccagttgcag
     2761 gcgcatattc cccagctcac ccgcgtggcc cgtgcgctgg ccgcactcga tgcgctgtgt
     2821 gcgctgaccg aacgcgcgtt gacgctggac tggaacgccc cgcaatttgt cgccgaaccc
     2881 tgcattgaca tcacgcaggg gcgccacccg gtggtgcagg cgcggctggc cgaactatcc
     2941 agtggcaact tcatcgccaa cgacacccgg ttgggtccca aggcgcgcat gcagatcatc
     3001 accggcccca acatgggcgg taagtccacc tacatgcgcc aggttgccct catcgtgctg
     3061 cttgccagca tgggcagcta tgtgccggca agcgcctgcc gcctggggcc gatcgatgcc
     3121 atccacaccc gcattggcgc agccgacgat ctggccaacg cccagtccac cttcatgctg
     3181 gagatgaccg agggtgcaca aatcctgcac agcgccacac cgcacagcct ggtgctgatg
     3241 gacgagatcg gccgcggcac cagcaccttt gacggcctgg ccctggccag tgccatcgcc
     3301 acccaactgc acgacaagac gcaggctttc accctgttcg ccacgcacta cttcgaactt
     3361 accgagttcc ccgccacgca ccacgcggcg ctgaatgtcc acgtgagcgc agccgaagcc
     3421 ggccgcgaca ttgtgttcct gcacgccatc gagccgggtc ctgccagccg cagctacggc
     3481 atccaggtgg cgcgcctggc aggcatgccg gcggcggtgc tgaatcaggc acggcatacg
     3541 ctggaagccc tggaatccca cgccgcagac tcccaggcgc aggtagactt gttcgctgcg
     3601 ccgccagtga cggaaacagc aacagcttcg gctgtggaaa ccgcactggt ggccatcaac
     3661 cccgacgccc tgaccccgcg cgaggccctg gaagcgctgt accagctcaa agcgcttacc
     3721 cataaaaact agacgtcccg gcacaccggg gcgcgtgagg agacagacaa tgacagagca
     3781 cctgcaaccg ggtgggctgg cgcatgccag caacctggac gccatcatca tcggaactgg
     3841 ctttgccgga ctgggcatgg ccatcgccct gaagcaacag ggcatcgaga attttgtcat
     3901 cctggaaaag acccaggatg tgggcggcgt ctggcgcgac aacagctacc ccggcgcggc
     3961 ctgcgacgtg ccttcgcacc tgtactcgtt ctcgtttgag cccaaccccg actggtcgcg
     4021 cgtgttcgcc acccagggcg aaattcacag ctacctgcag cactgcgccc gcaaatacgg
     4081 cctgctgccg cacgtgcgct ttggcgtcga gatgcgccag gcggagtttg atgagcgtca
     4141 ctcccgctgg acagtcacca ccgccgatgg acaacaattc acggccaagc tgctgatcac
     4201 agccaccggg caactcagcc gtccggtgct gccgcaactg cccggtctgg agaccttcaa
     4261 gggacataca ttccactcgg cccagtggga ccaccagtac gcgctggacg gcaaacgcgt
     4321 ggcggtggtc ggcaccggcg cctcggccat ccagtttgtg cccgccattg ccgaccgcgt
     4381 agcgcagctc tcggtgttcc agcgctcacc tgcctacatc cttcccaggc cggatcggcc
     4441 ctacaccggc ctggagaaaa agctgtttgc cttgccctgg ctcagcaagc ttcaccgtgc
     4501 aggcatctac ctcaagtacg agtcccgcgc actggcgttc acgcgcttcc agtggctgat
     4561 gaagcctgca ggactcatgc ccttccaccg tctgctcaaa cgccaggtac aggacccaac
     4621 gctgcgggcg aagatgacgc cggactacca gatcggctgc aagcgcatcc tgctgtccag
     4681 cgaatacttg gccacatttg ccaaacccca ggtggcgctg tgcaccgaag gcatccggcg
     4741 cgtcacgccc gagggcatcg agacccagga cggcaagttg caccccgccg atgcgatcgt
     4801 cttcggcacc ggctttgcgg ccaccgaatt cctggcgccc atgcgcatca caggtcgcca
     4861 gggcctggac ctgcagacag cgtggaaggg cggcgccaag gcctacctcg gactgacggt
     4921 accgggcttt cccaacttct tcatgctgta cgggcccaac accaacctgg ggcacaactc
     4981 catcgtctac atgctggaaa gccagattgc ccatgtggtg cgctgtctga aggagatgcg
     5041 cgccacgggc accagcacca tcgaggtgcg ggaagccccg ttccttcgct tcaacgcgca
     5101 tatccagcag cgcctcaaaa attcggtctg gagtgtctgc acaagctggt atgtcgatgc
     5161 ccaggggcac aacagcacca actggcccgg cttcacactg agctaccgct ggttgctcaa
     5221 acgcggcagt ctggatgctt accgcttttc agaaccccgc accgaggctg tcgccccgac
     5281 ggcagagatt gccgcaccca ccgacatcgt cgagcaggcg caatcgacat ttttgcgggg
     5341 ttttctgcgc gtggcgtttc gcactttcat cggtcctccg tatggagccg tggcgcaacg
     5401 gcgctgggtg aatgtgctgt ccatcctcat gccggcacgc tcgggtgtgc aacggcagac
     5461 caccgtgatc ggcaacatcc cggtcaccat cacccacccg aaggccacca cagccgacgg
     5521 cgtggtgctg tacctgcatg gtggcgcgtt ttgcgtggcc agcccgcaca cgcaccgcag
     5581 catcacctcg cacctggccc acgccagcgg catgccggtg tgggcaccgg actaccggct
     5641 ggcccccgaa cacccctatc cggctgcact ggaagacgcc accagcgtct atgaatttct
     5701 tctgcaacag ggctacgcgc cggctcgcat cgtgctggcg ggcgactcgg ccggtggcgc
     5761 actggcgctg gcgctggcca tccagttgcg cgaccaggac gcaccgcagc ccgccggtct
     5821 ggcgctgata tcgccggtca ccgaccccac cctcggtggc gccagcgtgc aggaacaggc
     5881 tggcaccgac cccatgatcc gcaagggctg ggtggaacag ggcgtggcct ggtacggctg
     5941 cccgccgggc acacccagcc atacgccgct ggcaacgtcc ttccaggagt tgccaccgat
     6001 gcacatccag gtcggcgacc gggagatcct gctgtcggat tcggtccgcc tggccgagca
     6061 tgcagtcgcc tgcggcgtgg attgccagct ggacatccac cgcgggcgct ggcacgtgtt
     6121 ccacctgcag gccctgttcc tgcgttcctc cgtgcaggca ctggaaacgc tggcgcaatt
     6181 cgcccgtgcg cgtgtccatg tcgctctgtc tacccctccc gccaaggaat tgccatgacc
     6241 caggaagccc gccgctgcat cttcatcact ggcgccgcca acggcatcgg gcgcgcctgc
     6301 gccaggctct ttgccagcca gggctggcgg gtcggtgtgg ccgacattga ccagtctgcg
     6361 ctggacacgc tggtgcagga actgggcacc gaaaaagcgc aggcatttcg cgtggacgtc
     6421 acacaagcgg aggactgggc cgttgcgctg gcggctttcc attcgcaatg cgggcgtctg
     6481 gatgtcctgc tgaacaacgc cggtatcctg atctccggcc cttttgccgc cagcccgctg
     6541 gcccagcacc atgcgctggt ggacgtgaac atcaagggcg tgctcaatgg ttgctacctg
     6601 gccaaacagt atctgcaggc cacgccagat tcccgcgtca tcaacctgtc gtccacctcg
     6661 gccgtctatg ggcaagcgtc gctggcaacc tattccagta ccaaattcgc catacgcggt
     6721 ttgatcgaag ccctgaacat cgagtggcag gacgatggca tccgtgtcat ggacatcatg
     6781 ccgctgtatg tgcgcaccgc catgctcaag gacatcaagg cgcgcagtgc cgaccgcctg
     6841 ggcgtgcagc tcacccccga agacgtggcc gccacggtgt ggaaggcggc caacttcacc
     6901 ggttcgtttg gcaaggtcca ctggccattg aactggcagg ccgtatggat gttccgcctg
     6961 accagcatga cgcccgactg gctggcccgc gccattgcgc gcaggattgc ccgctagagt
     7021 ctgccggggt ctgcggaacc ccacccgata cactctaggt tttgctccca cgaacctgga
     7081 aaaatgacct actgcgtcgc catcaaactc aacgccggcc tggtgttcct ctccgactcc
     7141 cgcaccaacg ccgggctcga ccagatcagc accttccgca agatgatcgt gtacgagaag
     7201 cccgacgacc gcttcatggt gctgctgtcc gccggcaacc tgagcatttc gcagtcggtg
     7261 cgcgaaattc tgcagatcga aaagctcaag gaccacgacg atgacgaagg catcaccatc
     7321 tggaacgcca aaagcatgtt cgatgcggcc cgggtgctgg gctcggccat ccgcaaggtg
     7381 cacgaccgcg atgccggctc gctcaagcag gccggggtcg acttcaacgt ttcgctgatt
     7441 tttggcggcc agatccaggg cgaaggcatg cgcctgtttc aggtgtattc cgcgggcaac
     7501 ttcatcgaag ccacgcctga gacgccctac ttccaggtgg gtgagtctaa atatggcaag
     7561 ccggtgctgg accgcgtcat cacgcccacc acgccactgg acgaggccgc caagtgcgcc
     7621 ctggtgtcga tggattccac gctcaagtcc aacctgtcgg tgggcctgcc gctggacatg
     7681 gtggtgtacg aagccaacac tttccacacc gacaaggtgg tgtgcattga cgagaacaat
     7741 ccctacttca aaatgctgca caacagctgg ggccagaaac tgcgcgaggt gttcgacagc
     7801 atcgaagacc cggcctggaa cggtggtcgc accgatgttc cactgctgat tccttcgacg
     7861 cgcagcctgc cgctgaagaa aatcaccacc cccgacgaaa agctcatcta gccccgattg
     7921 cccccatgcc gctgatcatt ttttcccacg ccaacagttt tcctgccagc acttacaagg
     7981 tcctgttcaa gagcctgcgt tcgcgcggct ttacggtcaa ggcacccgaa aagttcggac
     8041 atgacccgcg ctacccggtc accaacaact ggccccatgt ggtgcagcag ctggccgatt
     8101 tcgccctgcc cgaagtggac aaggctggtg agccagcgtg gctggtcggc cactcgctgg
     8161 gtgggttcct gagtctgatg gcagccgcac gctttcccga attggcgcgt ggcgtggtac
     8221 tgattgactc accgattctg ggtggctggc gctcgactgc ggtgggtgtt atgaagagca
     8281 cgcaagtgat gggctccatc tctcctggcg cagtcagccg ccagcggcgc gacagttggc
     8341 cctctgtgga agctgcctat gcacactttc gcaacaagaa ggctttcgcc aaatgggacg
     8401 accaggtgct gctggactat gtgacgtacg gcacgtctgc catcgatggc aaacgggtgc
     8461 tgggcttcca ccgtgatgtg gaaaccctgt tctacaacac cgtgcccgac aacctggacc
     8521 gcctgctgtc gcgccacccg gtcaagtgcc ccgtcacctt cattggcgga cgggcctctc
     8581 atgagatgaa gcaggtcggc atggccatga ccgaaaaaat caccaaaggc cgcaccatga
     8641 tgctcgatgg cagccacctg tttcccatgg agaagccgct ggctactgca gcggccattg
     8701 aggctgcgct gcgcaacatg gagtcctagg cgcgtccatg tccgaagact cgcccacacc
     8761 actgcatctg ctggaacaga agttccaggc cctgcaagcc gagcacgaca cgctcaagca
     8821 gcagaagaac gaggtcttcc accaggtctt ctaccggatt gccgagcgtg ccactgcggg
     8881 tctgtcgttt tacgactttc tgaaggaagt gcacaccctg atgggcgagc tgctgccggc
     8941 catcaacctg tatgtcgccc tgtgcaacca cgaccgcgac accgtgagct tcccctacta
     9001 cgtcgatgag cgtgatggcg atatctggca acgcgacgac acgccacgca aacggaacat
     9061 gacggaatac gtcctgcgca ccggcacacc ccagctgatc gacgcagccc ggtttatcgc
     9121 cttgcgtgac agcggtgaga tcacgcaagc cgctggcgac ctgaccttca ccagctggct
     9181 gggcgttccg ctgaccctgc acggacgtgt cagtggcgta ctggcagtac agagctacac
     9241 cgaaggtgtc agctacaccg acaaggacgc cgatgtactg gcctttgttg cccaccatgt
     9301 cagcagcgcg attgagcgct accaggccat cgacgcactg cgaaaatcgg aagagcgcta
     9361 ccgtctggtg atcgaaaaaa tcagtctggg ggtggccgtc attcaggacg gccgcatgat
     9421 ttttgccaac ccggccctgg tccgcattct gggttacccg ctggaatggc tgctgaccca
     9481 gcccttttcc gccattgccc atccggatga cgtaccggtc atggtcgagc ggcagcggcg
     9541 gcgcatgcgc ggcgaggccg ttcctgcgga ctacacgttt cgcagcatta cccaaacggg
     9601 tgaggtgcac cagctggaag tttcggccgt cctcattgaa tgggggcaac gtaccgccac
     9661 actggcgttc gttgccgatg ccaccacccg gctccatgca gagcaggcgc agcgcgtggc
     9721 gctggagcag caaactgaac tcaacgacat caagacccgg tttatttcga tgacctcgca
     9781 cgagttccgt accccactgg cctccatcca cggctctgtg gagctgttga tgcactacga
     9841 cgatcgcctg tcggtcgaca aaaagcgcca gatcctggaa aacatcgacg atgcggtgga
     9901 gcgcatgacc cacatgctgg gcaacgtgat gcagatgggc cgctccgatg ccgggcaatt
     9961 gcagtttcgc cccaaacctc tggcattcac gccgttttgc ctgtccctga tcgaagagct
    10021 gcgggaaacc atgccccgcc agtttgccaa agtccagctg gaactggacc tgctccccac
    10081 gaaacaccag ttcatgctgg acgagacgtt gatccgcaac atcgtcggca acctgctgtc
    10141 gaacgccttc aagtactccc cacggggtgg cacggtcacg tttgctgcac gcaagaagaa
    10201 gggcgagatt gtgctgaccg tttccgacca gggcatcggc attccccagg cggaccaagc
    10261 cctgctgttc cagacttttc accgcgccag caacgtgggc gccatcgccg gcaccgggtt
    10321 gggcctgtcc atcgtgaagg aagcggtcga ttgccaccag ggccgtatcg aagtgcaaag
    10381 caccgaaggc gaaggcagcc aatttaccgt tatgctgcca gcatctaccg taacggcctg
    10441 agacccacca cgtgcatatc ctgattgcag aagacgaacc ctcgctgcgc gaaaacctgc
    10501 aatggatgct ggaaatggaa ggcttcaccg tcgtggccgc cagcgatggt ctgcaggcgc
    10561 tggagtttgc ctgcctggca ccgcccagtc tggtattgac cgacgtgatg atgccgcacc
    10621 tggacggcta cggtctggtc aaggcactgc gcgaaaccat ggctacgtcg accgtgccca
    10681 tcatcatgct gaccgccaag gcggaccggc gcgatgtacg tatgggcatg aatctgggcg
    10741 ccgacgacta cctcaccaag ccctaccgcc gcgatgaact gctggctgcc gtgcgcgccc
    10801 ggttggcccg caaggccagc ctggatgaaa ccgcacggcg cctgcaggaa gaagcccgcg
    10861 aagcccagca acgcgacgcc ctgaccgccc tgccggggcg cgagctgttc ggccagcgtc
    10921 tggcggtgac gctgaagcag gctccagcgg aaaacctgca ctccatcgga ctggtgtgcc
    10981 tggggctcga cggattctcc aaagtcaacg actcgctggg cgcggccgtg ggcgacaagg
    11041 tcttgtacga agtggcctgc cgtctgggcg agcacatgcg cgatgcgcgg gacagcctgc
    11101 ggtacgactg tgtggcccgc ctgggcagcg acctgttcgc ggtgtacatt ggcgaaaaca
    11161 ccaatctgac cgcactggag gaatccagcg ctgcattgct gcaggtgctg gcccagccct
    11221 acctggtcga tggacacacc ctgtttctca ccgcctgcgc cggtgccagt ctgttcccga
    11281 tgcaggctgc cgatgcccat ggcctgctgc tcaatgccga gacggccctg caccacgcca
    11341 agccggtggg gccgggcacc gtacgcgtgt ttgacaccgc catgaacttg caggtggcac
    11401 gccgcctgca ggtgcacaac gccctgcatg ccgcgctgga gcagaatgag ctgcaggtgt
    11461 actaccagcc ccaagtgagc atcgccgctg gcaatgtggt ggggtttgag gcgctgctgc
    11521 gctggcacca ccccacgctg ggctcggtgt ccccggcaga gttcattccc atcgcggaag
    11581 agagcggtct gatcacccgc atcggtgcct ggaccatgcg cacggcggca gcccaggccg
    11641 cccagtggca cgcacaggga catggcacct tccgcatggc ggtcaacctc tcggtacgcc
    11701 agtttctggg cagcgacctg ccgggcctgg tccgcaccgt gctggaagaa accggcctgc
    11761 caccgcatat gctggagctc gaagtgaccg agagtctggc gctgcacagc gtctcctcca
    11821 cgctggccac cctgcacgcc tgcaaggccc tgggcgtcat gctggccatg gacgactttg
    11881 gtaccggcta ttccagcctg gcttacctca agcgctaccc gctggactgc ctgaagatcg
    11941 accagacctt tgtgcgcaac attacgcagg acattggcga cgcggcaatc acccgcgcca
    12001 tcgtggccat ggcgcacagc tttggcatgt cggtgattgc agagggtgtg gaaacgatcg
    12061 agcagctcga tttcctgcgc ggcctgggct gcgaggagtt tcaaggcttc ctgttcagca
    12121 aaccggttcc cgccgaagag gcggtgaaat gctttgcggg ctttatgccg cccagtgcac
    12181 cactttggtc caggccgtga ccagcaccac gataccgaac gcaatacggt agtaggcaaa
    12241 gcctgtgaag ctgtgggttg cgatatagcg cagcagccag cggatgcaca accaggcgct
    12301 cgcaaacgag aacaccagcc ccaccgcaaa catcggcaca tcggccacgc tgagcagcgc
    12361 acgctccttg tacaggctgt agacgcccgc cccgatcagt gtcggaatag ccaggtagaa
    12421 cgaaaaatcg gtggctgcct tgcgcgacag gcccagcagc atgccgccaa tgatggtcgc
    12481 cccactgcgg ctggtgcccg gcaccatggc cagacactgc accagaccca ccttgagggc
    12541 atccagggtg ctcatctcat ccacactgtg gatgcgcacg gcattgccgc ccgccgcctg
    12601 ccggcgctcc gcccacagga tgatgaagcc ccccacaatg aacgtggtcg ccacaatggt
    12661 gggcgtgaag aggtgggcct tgatggcctt gcccagcagc aggcccagca ccacggcggg
    12721 cacaaacgca atcagcacat tcagcgcgaa ttgctgcgcc tgcttctggc tgggcaaggc
    12781 aatgagcgtg tcgcggatct tctgccagta caccaggatc accgcaaaga tggccccggt
    12841 ctggatggcg atgtcaaaca ccttggcctt gtcgtcgtca aaacccagca gggccccggc
    12901 gaggatgagg tggccggtac ttgaaatcgg caggaactcg gtcagcccct ccaccacgcc
    12961 catgatggcg gccttgatca acaatgaaaa atccaacact ttctccctgt attcagaaca
    13021 aaattggcct ctagcccccg tattatctgc gcaagacgct cacttattca tagcgcaatg
    13081 cctggatagg caataacttc gatgcccggc gcgccgggta gtagccaaag aaaatgccca
    13141 ccacgcccga gaaacccacg gccagcagca cggcccccag gctcaggctc acgttccagc
    13201 cggcgaacgc gcccaccgcc caggtcgcta cggcccccag ccccaccccg atcgccccgc
    13261 caatcagact cagcgtgacg gcttcgatca gaaactgggc caggatgtct ttgccgcgcg
    13321 cacccactgc caggcgcaag ccgatctcgc gggtgcgctc ggtcacgctc accagcatga
    13381 tgttcatgat gccg
//
LOCUS       DAOSJM010000014        13179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4773_length_13179_cov_4.629000, whole genome shotgun sequence.
ACCESSION   DAOSJM010000014 DAOSJM010000000
VERSION     DAOSJM010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13179
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4773_length_13179_cov_4.629000"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /gene="glcE"
                     /locus_tag="PKX82_01080"
     CDS             complement(<1..1096)
                     /gene="glcE"
                     /locus_tag="PKX82_01080"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HOF50866.1"
                     /translation="MEYALAAVTDRIRTALAGGGPLRIRGGGSKDFYAQPLTGEVLDT
                     TGLRGVVSYEPSELVVTVGAGTPLVELEALLAEQGQCLPFEPPHYAWSQPDGASRATV
                     GGMVAAGLAGPARASVGGVRDYVLGLKLINGQAQHLTFGGQVMKNVAGYDVSRLMVGA
                     MGTLGLLTEVSLKVLPVAPAEATLVFEVDQAAGLEQLHRWGGEPLPLNASCWVHDDSD
                     PAAAPKDLLFVRLRGAVAAVEAACKRMLSDLPGSRMDNAQAGPDWALCRDQKLPFFHR
                     PADASLALWRLSVPQTAPVLDLPWAQLIEWHGGLRWLWAPHDAAAQLQTVARQANGNA
                     TVFVAAHANSTGTGGRFGVHGLAEAAIERRL"
     gene            complement(1173..2084)
                     /locus_tag="PKX82_01085"
     CDS             complement(1173..2084)
                     /locus_tag="PKX82_01085"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOF50867.1"
                     /translation="MSWQALTAQTCALGESPFWHPHEQMLYWVDIPGRQLLRANIYMG
                     TVQAWDMPSEPGCIAPAASGGLVVALRDGVFRAREWGGALERITTLPYDTAVVRANDG
                     KCDPAGRFWVGTIDEAKKAHAAQLFSIDCRQGAPVVECKAGNALTANGLAWSPDAQTL
                     YWADTSSHVVHAWDYDLASNAMTAHRVHLTFTAKPEGWSFGQGGYGGRPDGAAVDVDG
                     NYFVAMYEGARICQFAPDGTLLAEFPTPAQCPTMPCLGGEDLRTLYVTTGRHGRSAAE
                     LEQYPQSGCVFSMRVDTPGLPVNFFAE"
     gene            complement(2081..2956)
                     /locus_tag="PKX82_01090"
     CDS             complement(2081..2956)
                     /locus_tag="PKX82_01090"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HOF50868.1"
                     /translation="MNTTPSPVSLRCDALRCEIKPELGGCIAGLWLGDIPVLRSTPAQ
                     GLHSVRQSASYPLVPFSNRVGHASLQWQGTDHPLVKNFEPEPHAIHGVGWERPWAVLE
                     ASDTFALLSYEHKADAAWPFDFDSSQAFKLNAHALEMTLSITNQSKVSAPVGLGWHPY
                     FVKRPGSRVTFSTLGRWEMGDDKLPTHRTDSAGLDDDCAALAVDHCFDGWRGELHLRD
                     ALLHTRVSSSLTRLVVFTQPGRDSIAIEPVSHVNNAVHLMVAGIATAEELGLVILKPG
                     ETFSCEMRMDVERVA"
     gene            complement(3083..4618)
                     /gene="amt"
                     /locus_tag="PKX82_01095"
     CDS             complement(3083..4618)
                     /gene="amt"
                     /locus_tag="PKX82_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOF50869.1"
                     /translation="MKKLLVSLALGIGLLTGGTFALAQTAAPAAAEASAPVAAPAAAA
                     DAAAPAAAPAAAEAAAPAAAPAPVPNKGDTSWMMVSTLLVILMTIPGLALFYGGLVRS
                     KNMLSVLMQVMVTFSLIVVLWFIYGYSLAFTEGNAFIGGFDRLFMKGVWDNAAGTFAN
                     AATFSKGVYIPEIVFAAFQATFAGITGALIVGAFAERMKFSAVLAFMVLWFTFSYAPI
                     AHMVWFWMGPDAYSAAAVVDEMNGKAGYLWQMGALDFAGGTVVHINAAVAGLVGAFMV
                     GKRIGYGKEAMAPHSLTLTMVGAALLWVGWFGFNAGSALEANGFAALAFINTLGATAA
                     AVLAWSLGESLMRGKASMLGAASGAVAGLVAITPAAGNVGIGGALIIGLLSGFAGLWG
                     VNGLKKMLGADDSLDVFGVHGVCGILGAILTGVFNSPNLGGPSLVADWTTVAMVTADA
                     YTIAGQVWTQTKAVLITVAWSGIVSVIAFKLVDLTIGLRVSEEDEREGLDITSHGETA
                     YNR"
     gene            complement(4638..4976)
                     /gene="glnK"
                     /locus_tag="PKX82_01100"
     CDS             complement(4638..4976)
                     /gene="glnK"
                     /locus_tag="PKX82_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOF50870.1"
                     /translation="MKLVTAIIKPFKLDEVREALSSIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAIDDALVERVIEAIEGAARTGKIGDGKIFVYDVEQVVRI
                     RTGETGKEAL"
     gene            5227..6789
                     /locus_tag="PKX82_01105"
     CDS             5227..6789
                     /locus_tag="PKX82_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOF50871.1"
                     /translation="MSLSLVQSRALLGLEAAKVTVEVHLANGLPSFTLVGLADVEVKE
                     ARERVRCAIQNANLEFPTNKRITVNLAPADLPKDSGRLDLPIALGILAASGQIDAARL
                     AEYEFAGELSLSGELRPVRGALATALALHTGQVSAKLVLPPGSAEEAALVPGAQVYRA
                     RHLLDVVRHFAPPGSPALEGQDSATAASDDGWARVHAAAPTASARIADMADVKGQAGA
                     RRALEIAAAGGHSVLLVGPPGSGKSMLAQRFAGLLPEMTTDEALQSAAIGSLNGRFSL
                     ERWGQRPTCSPHHSASAVALVGGGSPPRPGEISLAHHGVLFLDELPEFPRAALEALRE
                     PLETGSITISRAARRAEFPARFQLIGAMNPCPCGYLGSTTHACRCTPDQVHRYQSKLS
                     GPLMDRIDLHVEVPALPAGDLLNAPSGESTAHIQQRCHAARERAARRQGKTNQALAGQ
                     DIDTHARLDPAALKFLQSASAQLGWSARGTHRALKIARTIADLADSEATQLTHVAEAM
                     QYRRALQRAVGP"
     gene            6803..8041
                     /locus_tag="PKX82_01110"
     CDS             6803..8041
                     /locus_tag="PKX82_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF50872.1"
                     /translation="MTHRTPSAPAVYSASWIGWLSLAQLISWGSLFYTFTLLMGPVEQ
                     ELGLSRVQSSLAFSLALLAEGLMAYPVGRWIDRGHERRVMTAGSLLAGTCLLLHGLVT
                     TATQFYAVWMVLGVSMAATLYTPVFAVVTRRFPNDFRRAIITMTFLGGLASTVFIPLT
                     AWLMSALGWRHALWCLAALHLLVCTPMHYRLLRCAPGRRPACGHSAGVPGKTLSHHML
                     SAPFLLVATFIVLMMAVTVALPAHMVSLLREHNLSEAWVIAIPASIGVIQVLGRLLLY
                     FFERHFDLHLANRLIPALIPLGLLALIAAPWMEATHGALVLLFVLVWGVGNGMLTIVK
                     GTAIAQYVDRDHVASLNGAMGLPLAFARAGAPLGLGYLWSPQAGYTTGLWVLLGISVA
                     GVGALTLAQRHSTHHQNATK"
     gene            complement(8088..9221)
                     /locus_tag="PKX82_01115"
     CDS             complement(8088..9221)
                     /locus_tag="PKX82_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50873.1"
                     /translation="MSVINKVLRDLDKRQGTPQARATSEDLGARIGTSSVDALELPQR
                     SRPAAPIRWGGVAGSGLLLAALGAGWVLWQSGALDGLLSGRVYVAPTAVPAPPSTPAA
                     APTVAVAPAEQVPVLPAEPAASAPELPAMELAPASRFESTLALPKDLQPAEPAKVPAN
                     PVKAPVAPVATRSMASPPAPAQAVAPAPVAVPKPAVAPAPAAAELAQRQQQAGRDALA
                     HAQSLWNAGSRESAIELLQQAVASTERAATSAPGPATTQTLVLLVRELGRMQLSDGRP
                     GAVWETLTRLEPLLRNEPEMWALRANAAQRLGRHQDSVYAYMTALQSRPAEQRWLLGA
                     AVSLAALGQIASSAEMAEKARAAGPISKEVQAYLRQMGVPLKD"
     gene            complement(9218..10141)
                     /locus_tag="PKX82_01120"
     CDS             complement(9218..10141)
                     /locus_tag="PKX82_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF50874.1"
                     /translation="MYLGHFSLREAPFSITPDTDFFYPHEGAQASLNMLLVALRSGEG
                     FVKVVGELGCGKTVLCRQLLKTLHGEFVTAYIPNPDMGPEDLLVALAAELGLAVDPQA
                     SRHQIMGALHTCLLDHAKAGRKVLVCIDEAQAIPVRTVESLRLLSNLETEKRKLLQLV
                     LLGQPDLDDKLARPEIRQLLQRITFSEYLGPMLAKRVPAYLEHRIARAATDSTVDTRL
                     FKPRAAIAIAHYSGGVPRLINVMANKCLMLAYGEDVQRVTVEHVHLAAKDTPGVVARR
                     PWWLWMRPALDLRKAPPKPRSPAPDRKGRTT"
     gene            complement(10141..11889)
                     /locus_tag="PKX82_01125"
     CDS             complement(10141..11889)
                     /locus_tag="PKX82_01125"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOF50875.1"
                     /translation="MSYPVVISTVFPRSPVLSCLALAVAALGGCSMDRPLRTPDVSAA
                     IRAELPPEAPAVAQAKPAAVPARVMDALAEPAPPAQPLPPEPRIDLLVNNAQAREVFL
                     AIVADTRYSMLMHPDVKGTLSVTLRGVTVTEALESIRDVYGYDFKMEGRRITIYAPTL
                     QTRVFTVNYPNSQRTGNSETRVSSGGGQTSTAGTTGAAGATGSSNQQPESSRVTTSSK
                     TDFWAELAGAVKGMVGNGEGRSVITSPQAGIMAVRAMPDELRQVEKFLKAAQIAVERQ
                     VMLEAKVIQVELSDGYQSGVNWAAFGSDGTTRVLTGVLGSGVANTEALLQTGAAISGT
                     VAIPSVATGGGLFGLALSTTNFAAVLGFLETQGDVQTLSSPRVATLNNQKAVLKVGTD
                     EFFVTNVSGGTATSGSTSTTTNSTTMPTVTLTQFFSGISLDVTPQVDDGTSVTLHVHP
                     SLTSVTEKTKQIDLGSVGNYKLPVASSSVNETDTLVRIQDGSIVAIGGLMQMESSRSA
                     SGLPGTTGLGVFSALFGNKAVVGRKKEVIVLIKPTIIRTAADWESQNQRTRAALNDMD
                     AARARVIQIDGSSTAK"
     gene            complement(11901..12350)
                     /locus_tag="PKX82_01130"
     CDS             complement(11901..12350)
                     /locus_tag="PKX82_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50876.1"
                     /translation="MKYPMWHRVVLLGFITLMASALHAQALRDPTQPPAMAGMGPDGS
                     VAADAPLGDGVAVVVRDGKSFLVSGTRLYGVGQKLGQFRIERISETEVWLRNGNDLRK
                     LPRFAGVQRSASKPAEQCGTQPAKSVKSSKTPSSTSPKAAQCAGAQP"
     gene            complement(12347..12976)
                     /locus_tag="PKX82_01135"
     CDS             complement(12347..12976)
                     /locus_tag="PKX82_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50877.1"
                     /translation="MKQQWLKLLERIDALSLRERVFLFVSLIPCMLALVDVLWLSPVQ
                     TEHKQLSQRNASQASELERLRAELKTVARPVDPSKAVRDDIAAADTRLDALNIEIKSL
                     IPMAQNGPALEQVLVQLLRRQDGLVLQGLTTLKAESAVATGAQAALPPGMVKRGLELR
                     VTGPYPDLVRYVKSLETALPALRWGALNLKSDQQPPELSLQVYVVGVQP"
     gene            complement(12973..>13179)
                     /locus_tag="PKX82_01140"
     CDS             complement(12973..>13179)
                     /locus_tag="PKX82_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50878.1"
                     /translation="RMAASPLMRDLKLSTVTVENTVASRLSVPVAASATVPVAAGKTT
                     APRSVWSFSLVSLEPQPLASGSKP"
BASE COUNT         2417 a         4346 c         4077 g         2339 t
ORIGIN      
        1 tgaggcgccg ctcgatggct gcttcggcca gtccatgaac accaaatctg cctccagtcc
       61 ccgtggaatt tgcgtgagca gctacaaaaa cagtagcatt tccgtttgct tgacgggcta
      121 cagtttgcag ttgggcagcc gcgtcgtggg gcgcccacag ccagcgcaag ccgccatgcc
      181 actcaatgag ctgagcccag ggcaggtcca gcaccggtgc ggtctgcggc acgctcaggc
      241 gccacaaggc cagcgaagca tccgccggcc gatgaaagaa cggcagcttc tggtcccggc
      301 acagcgccca gtcgggcccg gcctgcgcgt tgtccatacg ggaaccgggc agatcggaca
      361 gcatgcgctt gcacgcagcc tccaccgcag ccacggcgcc acgcaggcgc acaaacagta
      421 aatccttggg cgcagcagcg ggatcggaat cgtcatgcac ccagcaactg gcattcaggg
      481 gcaggggctc gccaccccag cggtgcaact gctccagccc cgcggcctgg tccacctcaa
      541 acaccagcgt cgcctcggca ggcgcgaccg gcagcacctt gagcgacacc tcggtgagta
      601 gccccagcgt acccatggcg cccaccatca ggcgcgacac gtcgtagccc gccacattct
      661 tcatgacctg gccaccaaag gtcagatgct gcgcctggcc gttgatgagt ttgaggccca
      721 gcacatagtc ccgcacaccg cccacactgg cgcgggccgg gccggccagg cctgcagcca
      781 ccatgccacc cacagtggcc cgggatgcgc cgtcgggctg ggaccaggca tagtgcggag
      841 gctcaaacgg caggcattgc ccctgctctg ccagcagggc ttccagctcc accagcggag
      901 tccccgcgcc gaccgtgacc accagttcgc tgggctcgta gctcaccacg ccgcgcaggc
      961 cagtggtgtc cagcacctcg ccagtcagcg gctgggcata aaagtctttg ctgcccccac
     1021 cccggatgcg cagcgggccc cctccggcca gggcggtccg aatgcggtcg gtgactgcgg
     1081 cgagtgcgta ttccatggat tgcatggcag cgatggtaaa tgcccgtcag cgctgtgggc
     1141 gtgaattttt tgaaacaccc cggttcaaaa aactattctg caaaaaaatt gaccggcaag
     1201 cccggtgtat cgacccgcat ggaaaacacg cagcccgact gcggatactg ctccagctcg
     1261 gcggcactgc ggccatgccg gcccgtggtg acatacaggg tacgcaggtc ttcaccgccc
     1321 aaacagggca tggtggggca ctgtgccggg gtcggaaact cggccagcaa cgtaccgtcc
     1381 ggcgcaaatt ggcagatacg cgcgccctcg tacatggcca cgaagtagtt gccgtccaca
     1441 tccaccgccg caccgtcagg gcggccgcca tagccccctt gcccaaagct ccagccttcc
     1501 ggtttggccg tgaatgtcag gtgaacacga tgggccgtca tcgcattgct ggcgaggtcg
     1561 taatcccagg catgcaccac gtggctggac gtatccgccc agtagagggt ttgggcgtcc
     1621 gggctccagg ccaggccatt cgccgtcagc gcgttgccgg ctttgcactc caccacgggg
     1681 gcaccttgac ggcagtcgat ggaaaagagc tgggcggcat gggccttctt ggcctcatcg
     1741 atcgtaccga cccagaaccg gccggcggga tcgcatttgc cgtcgttggc acgcaccacc
     1801 gccgtgtcat acggcagggt agtgatccgc tccagtgcgc cgccccactc gcgggcacga
     1861 aaaacaccat cgcgcagcgc caccaccagc ccaccactgg cagcgggagc gatacacccc
     1921 ggctcactgg gcatgtccca ggcctgcacc gtgcccatgt aaatattggc gcgcaggagc
     1981 tgcctgccgg gaatatcgac ccagtacagc atctgctcat ggggatgcca gaacggcgac
     2041 tcgcccagcg cacaggtctg cgctgtcagt gcctgccagc tcacgccacg cgctccacgt
     2101 ccatgcgcat ttcgcaggaa aaggtttcgc ccggtttcag gatgaccagg cccagctctt
     2161 cggccgtggc aatgccggcg accatcaggt ggacggcgtt gttcacgtgg ctcaccggct
     2221 cgatggcgat gctgtcgcgc ccgggttggg tgaataccac caggcgcgtc aggctggaac
     2281 tgacccgcgt atgcagcagc gcatcgcgca aatgcaactc cccccgccag ccatcaaagc
     2341 agtggtccac cgcaagcgct gcacagtcat cgtccagacc ggcactgtcg gtgcggtggg
     2401 tcggcagctt gtcgtccccc atttcccaac ggcccagcgt tgaaaacgtg accctgctgc
     2461 caggccgctt gacgaagtag gggtgccagc ccagccccac cggcgcggac actttggact
     2521 ggttggtgat gctcagcgtc atctccagcg catgggcatt cagcttgaaa gcctgggagc
     2581 tgtcaaagtc gaacggccac gccgcatccg ccttgtgctc ataggacagc agggcaaagg
     2641 tatcgctggc ttccagcacg gcccagggcc gctcccagcc cacaccatgg atggcatgcg
     2701 gctcgggctc gaagttcttc accagcggat ggtctgtgcc ctgccactgc aggctggcgt
     2761 ggcccacccg gttggaaaac ggcaccagcg gatagctcgc cgattgccgc acggaatgca
     2821 gaccttgcgc tggcgtggag cgcaacaccg gtatatcccc caaccacagg ccggcgatgc
     2881 agccccccag ttcgggtttg atttcacagc gcagcgcgtc gcagcgcaag gacacagggg
     2941 acggcgtggt attcataggc aaattgtgca ccgcaatcgc ccaaaaaaat ggcccggagg
     3001 aacttcgggg ttaccgaagc ctttccgggc cgcagcctcc atcctctgga gactgctgga
     3061 gcgtgacagc ttgtttgtct gtttagcggt tgtaagccgt ttcgccgtgg ctggtgatgt
     3121 ccagaccttc gcgctcgtct tcctcgctga cacgcagacc aatggtcagg tccaccagct
     3181 tgaaggcgat gacggaaaca ataccagacc atgccaccgt gatcagcacg gccttggttt
     3241 gtgtccagac ctggccggca atggtgtatg cgtcagcagt caccatggcc accgtagtcc
     3301 agtcagcgac caggctcggg ccacccaggt taggcgagtt gaacacgccg gtcaggatcg
     3361 cacccagaat gccgcaaaca ccgtgcacgc cgaacacatc cagcgagtcg tctgcaccca
     3421 gcattttctt cagaccattc acaccccaca gaccagcaaa gccggacagc agaccgatga
     3481 tcagcgcacc gccgatgccg acgttaccgg cagcaggggt aatggccacc aggccggcca
     3541 ccgcaccgga cgcagcgccc agcatggaag ccttgccacg catcagcgac tcacccaggg
     3601 accaggccag cacagcagcg gcggttgcac ccagggtgtt gatgaaggcc agggcggcga
     3661 agccgttggc ttccagcgcg gagccggcgt tgaagccgaa ccagcccacc cacagcaggg
     3721 cggcaccgac catggtcaat gtcaggctgt gaggcgccat ggcttccttg ccgtaaccga
     3781 tacgcttgcc aaccatgaaa gcaccgacca ggccagccac agcagcgttg atgtgcacca
     3841 cggtaccgcc agcgaagtcc agcgcgccca tttgccacag gtaaccggct ttgccgttca
     3901 tctcgtccac caccgcagcg gcagagtacg catcaggacc catccagaac cacaccatgt
     3961 gggcgatagg agcgtaagag aaagtgaacc acagcaccat gaaggccagc acggcagaga
     4021 acttcatgcg ctcggcaaac gcgcccacaa tcagtgcacc cgtgataccg gcgaaggtcg
     4081 cctggaaggc ggcaaacacg atttcaggga tatagacacc cttgctgaag gtcgctgcat
     4141 tggcaaaggt gccggctgcg ttgtcccaga cgcccttcat gaacaggcgg tcaaagccac
     4201 caatgaaggc attgccctcg gtaaacgcca ggctgtagcc gtagatgaac cacagcacca
     4261 cgatcagcga gaacgtcacc atgacctgca tcagcacgga cagcatgttc ttgctgcgca
     4321 ccaggccacc gtagaacagg gccagaccag ggatcgtcat caggatcacc agcaaggtgg
     4381 agaccatcat ccaggaagtg tcgcccttgt tgggcacggg tgctggcgca gctgcgggtg
     4441 cagcagcttc agcggcggca ggcgctgctg cgggtgcggc agcgtcggca gcagcagccg
     4501 gagctgcaac aggagcagag gcctcagcag ccgcgggggc agcggtctgg gccagcgcaa
     4561 acgtaccacc ggtcaacaga ccgatgccca gcgcgaggga tacaagcagt tttttcattt
     4621 ttgggctctc tttctggtta cagcgcttct ttgccggttt cgccggtacg aatgcgcacg
     4681 acctgttcga cgtcgtagac aaaaatcttg ccgtcgccga tctttccggt gcgtgctgca
     4741 ccttcgatgg cttcgatcac gcgctcgacc agagcgtcgt cgatggctgc ttcaatcttc
     4801 accttgggca gaaagtccac cacgtactct gcgccgcggt agagttcggt gtgtcctttt
     4861 tgacgcccga agcccttgac ttcagtgacg gtgatgccct gtacgccaat actggacaag
     4921 gcttctcgca cctcatcgag cttgaacggt ttgatgatgg ccgttacgag tttcatatgt
     4981 tcacctcaac ttaataacaa tgggttgcag tcacagaaaa gtgctgccct gcttctccag
     5041 gagtcaggag acaactcctc taagcagaac ccgtgccaac cgctcgttgg cccatttcat
     5101 cgcgtgcggt gcaccgtgtt cacgcgatat gcgctaccat actgtataaa taaccagcat
     5161 gcaccagttg ggtgcggaat tttgcagcgc accgcaatgt tgcgcactga aatgggagga
     5221 tgggaaatga gcttgtcttt ggtgcagagc cgtgctctgc tgggtctgga ggcggccaag
     5281 gtcaccgtag aggtgcacct ggccaacggt ttgcccagtt ttaccctggt cggtttggcg
     5341 gatgtggagg tgaaagaagc gcgcgaacgg gtgcgttgcg ccatccagaa cgccaatctg
     5401 gaattcccga cgaacaaacg catcaccgtc aatctggccc cggcggattt gcccaaggac
     5461 tccgggaggc tggacctgcc gattgccctg ggcattctgg cggccagtgg gcaaatcgac
     5521 gctgcccggc tcgcagagta cgagtttgcc ggggaactgt cgctctcggg cgaactgcgt
     5581 ccggtgcgcg gggcactggc cactgcgctg gcattgcaca ccggccaggt cagcgccaaa
     5641 ctggtgctgc caccgggaag cgccgaagag gctgcactgg tgcccggtgc acaggtgtac
     5701 cgggcgcggc atctgctgga cgtggtgcgg cactttgctc cgcccggttc acccgccctg
     5761 gagggccagg atagtgccac ggctgcgagt gacgacggct gggcgcgcgt ccatgccgct
     5821 gcacccacgg cgtcggccag gatcgcagac atggcggatg tgaaaggcca ggccggcgcc
     5881 cggcgtgccc tggaaattgc tgccgcagga gggcacagcg ttttgttggt gggtcctcca
     5941 ggctcgggca aatccatgct ggcccagcgt tttgccgggc tgctgcccga gatgacaacg
     6001 gatgaagccc tgcaaagtgc cgccatcggc agcctcaacg gccgtttttc gctggagcgc
     6061 tggggccagc gaccgacctg cagcccacac cactcggcca gtgccgtcgc gctggtgggt
     6121 gggggctcgc cgccgcggcc cggagaaata tccctcgcgc accacggcgt cctctttctt
     6181 gacgaattgc ccgaatttcc aagagcggcc ctggaagccc tgcgcgagcc gctggagacc
     6241 gggagcatca ccatctcgcg cgcagcgcgg cgtgccgaat ttccggcgcg cttccagctc
     6301 ataggcgcca tgaacccctg cccctgtggc tacctgggct ccaccaccca tgcctgccgt
     6361 tgcacgcccg accaggtgca ccgctaccag tccaaactca gcgggccgtt gatggaccgc
     6421 attgacctgc atgtggaagt ccccgccctg cctgccggcg atctgctcaa tgcgcccagc
     6481 ggcgaatcca ctgcccacat ccagcagcgt tgccacgcgg cccgggagcg cgccgcacgg
     6541 cgccagggca agaccaacca ggcactggcc ggccaggaca tcgataccca tgcccggctg
     6601 gatcctgccg cgctcaagtt cctgcagtcg gcatccgcac agctgggatg gtccgcgcgc
     6661 gggacccacc gggcgctgaa gattgcccgc acgattgcgg acctggccga ctccgaagcc
     6721 acccaactca cgcatgtggc agaagccatg cagtaccggc gggcactgca gcgcgcggtc
     6781 ggtccctagc cataatcacc tggtgaccca ccgcacccca tccgcccctg ccgtctactc
     6841 tgcatcctgg atcgggtggc tgtcgctggc gcaactcatc agctggggta gcctgttcta
     6901 taccttcacc ctgctcatgg ggccggtgga gcaggagctg ggcttgagcc gtgtgcagtc
     6961 gtcgctcgca ttcagcctgg cgctgctggc cgagggcctc atggcctatc cggtgggccg
     7021 ctggattgac cgcggccacg agcgcagggt catgaccgca ggctccctgc tggccggcac
     7081 ctgcctgctg ctgcacgggc tggtgaccac ggccacgcag ttttatgcgg tgtggatggt
     7141 tctgggggtg tccatggcgg ccacgctgta cacgccggtc tttgccgtgg tcacgcgccg
     7201 ttttcccaac gactttcgcc gcgccatcat caccatgacc ttcctgggcg gactggccag
     7261 taccgtgttc atcccgttga ccgcatggct gatgtccgcc ctgggctggc ggcacgcgct
     7321 ctggtgtctt gccgcgttgc atctgctggt gtgcaccccc atgcactatc ggctgctgcg
     7381 gtgtgctccc ggacgccgcc ccgcctgtgg ccacagcgcc ggtgtgcccg gcaaaaccct
     7441 gtcgcaccat atgctcagtg cacccttcct gctggtcgcg accttcatcg tgttgatgat
     7501 ggcggtgacc gttgcattac cagcccacat ggtgagcctg ctgcgtgagc acaacctgtc
     7561 cgaggcctgg gtgattgcga taccggcgag cattggcgtc atccaggtgc tggggcgcct
     7621 gctgctgtat ttctttgagc gccatttcga cctgcacctg gccaaccggc tgattcccgc
     7681 cctcattccc ctggggttgc tcgccctgat tgcagcgccc tggatggaag ccacccatgg
     7741 cgccctggta ttgctgttcg tgctggtctg gggagtaggc aacggcatgc tgaccatcgt
     7801 caagggcacc gcgattgccc agtatgtcga ccgcgaccac gtggcttcgc tcaacggtgc
     7861 catgggcttg ccgttggcgt ttgcacgtgc gggcgcacct ctgggtttgg gttacctgtg
     7921 gagtccgcaa gcgggctaca ccacgggact ctgggtgctg ctgggcatca gcgtagccgg
     7981 tgtgggtgca ctgacgctgg cccagcgcca ctcgacccac caccaaaatg ctacaaaata
     8041 gatagctgtc tgcgcaatga atccgggggc tagggcccga tttcatccta atccttgagt
     8101 ggaacaccca tctgccgcag gtaggcctgc acctctttgc tgatcggtcc cgcagcgcgc
     8161 gccttctcgg ccatctcggc ggaactggca atctggccca acgcagcgag cgacacggca
     8221 gcccccaaca gccagcgctg ttcagccggg cgcgattgca gtgcagtcat gtaggcgtac
     8281 acgctgtcct ggtggcgccc cagacgctgc gcggcgttgg cgcgcaatgc ccacatctcg
     8341 ggttcgttgc gcagcagggg ctccagccgg gtcagcgttt cccacacggc gcccggccgc
     8401 ccgtcactga gctgcatgcg ccccaattcc cgcaccagca gcaccagtgt ttgcgtggtg
     8461 gcagggccgg gcgcgctggt ggcagcccgc tcggtggagg ccaccgcctg ctgcagcaac
     8521 tctatcgccg actcgcgtga accggcgttc cacagggatt gcgcatgggc cagggcatca
     8581 cggcccgcct gttgctggcg ctgggccaac tctgcggctg cgggtgccgg cgccactgcc
     8641 ggtttgggca ctgccaccgg cgcgggcgcc acggcctgtg cgggcgcagg tggcgatgcc
     8701 atggagcgcg ttgccactgg tgcgacaggt gccttgaccg ggttggctgg tactttggct
     8761 ggctcagccg gttgcagatc tttcggcagt gccaatgtgg attcaaaccg gctggccgga
     8821 gccagttcca tggcaggcag ctcgggtgcg ctggctgcag gttcggccgg caacacggga
     8881 acctgctctg ctggggccac ggcaaccgtc ggagcggcgg ctggagtcga cgggggagca
     8941 ggtactgcag tgggggccac atacacacgt ccggaaagca gcccatccag cgccccgctt
     9001 tgccaaagca cccagccagc acccagcgcc gccaacaaca agccggaacc tgcaacgccc
     9061 ccccaacgga tgggggcggc gggcctggag cgctgcggca gttccagcgc atccacgctc
     9121 gacgtgccga tgcgcgcacc cagatcttcc gaagtggcgc gggcctgcgg ggtgccctgg
     9181 cgcttgtcca ggtcccgcag caccttgttg atcacactca tgtggtacgc cctttccggt
     9241 ccggcgccgg gctacgcggc ttgggtggcg ccttgcgcaa gtccagtgct ggccgcatcc
     9301 acagccacca cggacgccgt gcgaccactc cgggggtgtc tttggcagcc aggtgaacat
     9361 gctccaccgt gacgcgctgg acatcttcgc cataggccag catcaggcat ttgttggcca
     9421 tcacattgat caggcgtggc acaccgccgc tgtagtgtgc aatcgcaata gcggcccggg
     9481 gcttgaagag gcgcgtgtcc acggtgctgt cggtggcggc ccgggcaatg cggtgctcca
     9541 ggtacgcagg cacccgcttg gccagcatgg ggcccaggta ttcgctgaag gtaatgcgct
     9601 gcagcagctg gcgtatttcc gggcgggcca gtttgtcgtc cagatcaggc tggcccagca
     9661 acaccagctg cagcagcttg cgcttttcgg tttcgaggtt cgacagcaac cgcaggctct
     9721 cgacggtacg cacgggaatg gcctgcgcct cgtcaatgca caccagcact ttgcgcccgg
     9781 ctttggcatg gtccagcaga caggtgtgca gcgcgcccat gatctggtgg cggctggcct
     9841 ggggatctac cgccagcccc aactccgcag ccaatgccac gagcaagtcc tctggcccca
     9901 tgtccggatt cgggatgtag gcggtgacaa attccccgtg cagcgttttg agcagttgcc
     9961 ggcacagcac ggtcttgccg cagcccagtt cacccaccac tttgacaaac ccctcgccgc
    10021 tgcgcagggc gaccagcagc atgttgaggg acgcctgcgc gccctcatgg gggtaaaaga
    10081 agtccgtgtc cggggtaatc gaaaacgggg cttccctgag tgaaaaatgc cccagataca
    10141 ttacttggcc gtcgaactgc cgtcgatctg gatgacacgg gcgcgggccg cgtccatatc
    10201 attcagcgcg gcacgggtac gctggttctg ggactcccag tcggcagcgg tgcggatgat
    10261 ggtgggtttg atcagcacaa tcacttcctt cttgcgcccc accacggcct tgttgccaaa
    10321 cagcgcggag aagacgccca gacctgtagt gccaggcagg ccggatgcac ttctactgga
    10381 ctccatctgc atcagtccgc caatggccac gatggaacca tcctgaatcc gcaccagcgt
    10441 gtcggtttca ttgacgctgc tggacgcgac cggcagtttg tagttaccca cgctaccaag
    10501 atcaatctgc ttggtcttct cggtcacgct ggtgagcgaa gggtgcacgt gcagggtcac
    10561 actggttccg tcgtccacct gcggggtcac atccagcgag atacccgaga agaactgggt
    10621 aagggtcacc gtgggcatgg tggtgctgtt agtggtcgta gaggtactgc cagacgttgc
    10681 cgttccgcca gacacgttgg tcacaaagaa ctcatccgta ccgaccttga gcacggcctt
    10741 ctggttgttg agggtggcga cacgcggact ggagagagtt tgcacatcac cctgcgtttc
    10801 caggaaaccc agcactgcag caaagttagt ggtcgacagc gccaggccaa acagcccgcc
    10861 accggtagcc accgagggaa tcgccaccgt cccggagatg gccgcgccgg tctgcagcaa
    10921 tgcctcggtg tttgccaccc cgctgcccag cacgcccgtg aggacacggg tggtaccgtc
    10981 cgaaccaaag gccgcccagt tcacaccact ctgataacca tcgctcaact caacctgaat
    11041 gaccttggcc tccagcatga cctggcgctc cacggcaatc tgcgcggcct tcaggaattt
    11101 ttcaacctgc cgcagctcat ccggcatggc gcgcaccgcc atgatgccgg cctgcgggct
    11161 ggtaatcacg ctgcgccctt ccccgttgcc gaccatgccc ttcaccgctc cggccagttc
    11221 ggcccagaag tcggttttgg acgaggtcgt gacccggctg ctttcgggct gctgattgga
    11281 ggagccggtg gcacctgcag ccccggtcgt gcccgccgtg ctggtctgtc cgccaccgga
    11341 ggacacgcgg gtttcgctgt tgccggtgcg ctgtgagttc gggtaattca cggtgaacac
    11401 acgggtttgc aacgtgggcg catagatggt gatcctgcgg ccttccatct tgaagtcgta
    11461 gccatacaca tcgcggatcg actccagcgc ctcggtcacg gtgacaccgc gcaaagtcac
    11521 cgacagggtg cccttgacat ccggatgcat gagcatgctg tagcgggtat cagccacgat
    11581 ggccagaaac acttcgcgcg cctgggcgtt gttgaccagc aggtcaatgc gcggctcggg
    11641 gggcaagggt tgcgctggtg gcgcgggctc ggccagagcg tccatcaccc tggccggcac
    11701 tgcagcaggt ttggcctgtg caactgccgg ggcctccggc ggcagctcgg cgcgaatggc
    11761 ggcactgaca tccggtgtcc gcaaggggcg gtccatggag cagccaccta acgcagccac
    11821 ggccagcgcc aggcacgaca acaccggact gcgggggaaa acggttgata tgacaacagg
    11881 gtacgacatg gtgcaagacc tcacggttgg gcgcctgcgc actgagcggc tttgggcgag
    11941 gtggaactgg gtgttttgga cgatttcacg gattttgcag gttgggtgcc acactgttcc
    12001 gcaggcttgg aggcagagcg ctgcaccccg gcaaaccgtg gcagtttgcg caagtcattg
    12061 ccattgcgaa gccagacttc ggtttcggaa atgcgttcaa ttcgaaactg gcccaatttc
    12121 tggccgacac cgtaaagacg cgtaccgctc accagaaagg atttgccatc gcgtacgaca
    12181 acggccacgc catcacccag aggtgcgtcc gctgcgacac tgccatccgg tcccatcccg
    12241 gccatcgcag gcggctgggt ggggtcgcgc aatgcctgtg catgcaacgc gcttgccatc
    12301 agggtgatga agcccagcag gacgacccgg tgccacatgg gatatttcat ggttgcaccc
    12361 ccaccacgta cacctgcagg ctcagctcag ggggttgctg gtcactcttc aaattcagtg
    12421 ccccccagcg caacgctggc aaagccgttt ccagactttt cacataccga accaggtccg
    12481 ggtaggggcc cgtcacccgc aattccaacc cccgcttgac catgccgggt gggagtgccg
    12541 cttgcgcgcc agtggccacg gcagactccg ctttgagggt cgtcagtccc tgcaggacca
    12601 acccgtcctg acggcgcaac agttgcacca gcacctgttc cagggccggg ccattctgcg
    12661 ccatcggaat caggctcttg atttcaatat tcagggcgtc cagccgggtg tcagcggccg
    12721 caatatcgtc gcgcaccgcc ttggacgggt cgacggggcg cgccaccgtc ttcaactcag
    12781 cgcgcaggcg ctccagttcc gaagcctgcg aggcgttgcg ctgcgacagc tgcttgtgct
    12841 cggtctggac cggcgacagc cacagcacat ccaccagcgc caacatgcac ggaatcagcg
    12901 aaacgaacag gaaaacccgc tcacgcaggc tcagtgcatc aatgcgctcc agcagtttca
    12961 gccattgctg cttcatggct tgctccccga cgccaatggc tgcggctcca gactcacgag
    13021 gctgaaggac cacacagagc gcggtgcagt ggtcttgccc gccgcaaccg gcacggtcgc
    13081 gctggcggcc acaggcacag acaaccgtga ggcgaccgtg ttttctaccg tcaccgtact
    13141 gagcttgagg tcccgcatca gggggctggc tgccattcg
//
LOCUS       DAOSJM010000015        13088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4813_length_13088_cov_5.148930, whole genome shotgun sequence.
ACCESSION   DAOSJM010000015 DAOSJM010000000
VERSION     DAOSJM010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4813_length_13088_cov_5.148930"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKX82_01145"
     CDS             <1..208
                     /locus_tag="PKX82_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50879.1"
                     /translation="FIKVMDNLTIEPDFFGGAVQTFSATKRLGSDASRLSQIQDGKWK
                     TVSDYFGSTAKPAAAPAAKAPAKK"
     gene            282..1463
                     /locus_tag="PKX82_01150"
     CDS             282..1463
                     /locus_tag="PKX82_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF50880.1"
                     /translation="MKKLLALCFPLVLSASLAHAQQGVTKDEILIGSIQDLSGPIAGF
                     GKQARFGMQLAVDEINELGGINGRKLRVIFEDSGYDPKKAVLAAQKLVNQDKIFMMVG
                     HLGTAQNLATMPVLFEKNIVNFMPITSSKEMHEPFNRLKYAFGPSYFDQMRTSAPKLV
                     KEKGAKKVCTLYQDDEFGLEVMRGAEAGLKTIAMEFTEKTTYKRGATDFSSQVAKLQS
                     SGCDFVVLGTIVRETIGTIATARKMGYSPTFLGSNAAYTELVHRLGGPAMNGFYATNT
                     VQTPYLDDASQPIRFWATKYKTKFNEDPTVLSIYGYLFVDTFAKAATKAGSKLSTESF
                     IKAMDTMTIPPDIFGGTQSTFSPTKHFGTETSRMSQIQDGKWKVVSDYPNAPASSGTK
                     K"
     gene            complement(1470..1967)
                     /locus_tag="PKX82_01155"
     CDS             complement(1470..1967)
                     /locus_tag="PKX82_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOF50881.1"
                     /translation="MPKTSANPLSGQPVVFEEEFVKGLTRIFEESITFNKVLGLKIDA
                     ITPERVVAHIDMQPQLVGHYSFNRIHGGVISAGLDAMGGLAVMAAIGARHMDEPPMQR
                     LHRFSKLGTIDLRVDYLRPGIGERFELCAEVMRLGSRVASTRMEFLGADGKLLSTGAG
                     AYIVS"
     gene            complement(1977..3152)
                     /locus_tag="PKX82_01160"
     CDS             complement(1977..3152)
                     /locus_tag="PKX82_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF50882.1"
                     /translation="MSVYEIADEDIALVEDAARKFVQTEVAPHLGAWEEAGEIPRSLY
                     QRAAEMGWLEMGYPEEFGGTPSPWKLRNALTIALARAGGSGGLMASLFSHNIGLPPVV
                     RHGSPELQRKVIPDVLAGKKIAALAITEPGGGTDVSALKTTARREGDEYVIDGEKVFI
                     TSGVRADWLTVAVRTDLKNKGPMGISMVMVPGDAVGLSRSPLKKMGWLCSDTAQIRFD
                     GVRVPVSNLVGEEGSGFKMILTNFNGERLSMAALSLGFAEACYDEALSWAQQRKTFGT
                     ALIDHQVIRHKFMDMKMRIESTRAWLNAVSARADAGDRGDKSAKGAEWVAQVCLLKNH
                     ATQTMQFCADQGVQILGGMGFMRGTTCERVYREVKVMMIGGGAEEIMKELASRQLGL"
     gene            complement(3180..4355)
                     /locus_tag="PKX82_01165"
     CDS             complement(3180..4355)
                     /locus_tag="PKX82_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF50883.1"
                     /translation="MQFTHEHLEIQKTLKRFIDAEINPHVEEWEEAEMFPAHEVFKKL
                     GNLGLLGLTKPEAFGGAGLDYSYGMAMAEALGHIDCGGIPMAIGVQTDMCTPALARFG
                     SDALKREFLVPAIAGDTVGCIGVSEPGAGSDVAGIKTVARKDGDDYVITGQKMWITNS
                     LQADWMCMLVNTGDGPTHKNKSLVMVPLRENGKLRKGIEVARKIKKIGMQSSDTGLLY
                     FDEVRVPQRNLIGQEGAGFVYQMQQFQEERLWCAASALQALNNCIEWTVEWAQDRKMF
                     GGTLADQQWVQFRLAEMKTEVESLRALTYRACELYVAGQDVLELASMAKLKAGRLGRL
                     VPDGCLQFWGGMGFTWENKVSRMFRDSRLGSIGGGADEVMMGIIAKTMGVAKRPTRS"
     gene            complement(4364..5248)
                     /locus_tag="PKX82_01170"
     CDS             complement(4364..5248)
                     /locus_tag="PKX82_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF50884.1"
                     /translation="MSYGSVFAPGLFADKVMVVTGGGSGIGRCTAHELAALGATVVLV
                     GRKLEKLQKVQEEITQDGGRATMAACDIRQEESVKKTVADIVAEHGRVDGLVNNAGGQ
                     FMTPLEAISAKGWEAVVNTNLTGGFLMARECYLQSMSKNGGSIVNIVADMWGSMPGMG
                     HSGAARAGMVSFTETAAVEWAGRGVRVNAVAPGYIASSGMDNYPVEMAPMIREMAKTI
                     PAGRFGTEAETAAGIVFLLSPAASFVSGTTLRIDGARPQARMGYPMRVPDAATQERAA
                     VKPFNGFHRAHLPKVFQV"
     gene            complement(5248..5901)
                     /locus_tag="PKX82_01175"
     CDS             complement(5248..5901)
                     /locus_tag="PKX82_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOF50885.1"
                     /translation="MPDQAISAPEIPKRPRGRPRKTVDERDDGNRRHALLQAAAKLFR
                     RKGFAATTTRDIAAAAGMQSGSPFYHFKSKDALLYAVMEEGMGTALNFLRAVVAAPEY
                     NTLPSSERLRALVRTHFDVLLGPGNDFVPVMLYENRSLNSRQQKSLAKLIAQYESLWM
                     PVLVSLEQSGQLRAPAPLARLLILGALNWAVQWFDPKKGASLDTLTDAAISMFIKES"
     gene            complement(5965..6114)
                     /locus_tag="PKX82_01180"
     CDS             complement(5965..6114)
                     /locus_tag="PKX82_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50886.1"
                     /translation="MAKGQQKTNKEAKKPKKDTSAPKPPSGLSEPVRATITTAVIPRG
                     KLKNK"
     gene            6160..6564
                     /gene="arfB"
                     /locus_tag="PKX82_01185"
     CDS             6160..6564
                     /gene="arfB"
                     /locus_tag="PKX82_01185"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOF50887.1"
                     /translation="MTSPIAIAENEVQVTAVRAQGAGGQNVNKVSSAIHLRFDIPASS
                     LPQGVKDRLLALRDSRITQAGVLVLKAQQHRTQDMNRLDAFARLHELVNSVATPPRIR
                     HATKPTRASKQRRLEAKTQRSEIKAGRGKISD"
     gene            complement(6792..7556)
                     /locus_tag="PKX82_01190"
     CDS             complement(6792..7556)
                     /locus_tag="PKX82_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOF50888.1"
                     /translation="MSDILIDTAAGVMTLTINRLDKKNSLTQAMYGAMADALNQAAAD
                     AAVRAVVLQGHETIFSAGNDIGDFLNGPTSTLESPVYRFLQGIAQFPKPLVAAVCGPA
                     VGVGTTMLFHCDLVYAGDNAAFSMPFVNLGLCPEAASSLLVPQMMGYHRAAEALLLGE
                     PFMAEAAMEVGLVNRVVPPTEANAVAQAQARKLAAKPLSSLVETKRFMKKGQTSLVLA
                     QMAEEGASFGRMLREPAAREAFTAFMEKRKPDFSKV"
     gene            complement(7568..8374)
                     /locus_tag="PKX82_01195"
     CDS             complement(7568..8374)
                     /locus_tag="PKX82_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOF50889.1"
                     /translation="MTEHVFDQAIALSSQGDGLWAGQTHPAYANMVGPFGGITAAQVL
                     SAVLQHPQRLGEPVALTVNYCTGVADGAFEVHARPARTNRSTQHWVIEMRQQGQTVIT
                     ATAVTAARRETWSVDEEPMPVCPAPDQVPLPAQSPPLAWAQRYEMRALTGTLPGVWND
                     GGDSSLSQLWMRDNPPRPLDFASLTALSDLFFPRVFLRRARLVPVGTVSMTVYFHAST
                     EQLAQTGTGYLLGQARAQAFRNGYFDQTSQLWNEAGILLVTTHQIVYYKE"
     gene            complement(8429..9622)
                     /locus_tag="PKX82_01200"
     CDS             complement(8429..9622)
                     /locus_tag="PKX82_01200"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOF50890.1"
                     /translation="MKQVQDAYIVAATRTPIGRSGRGFFRNTRPDELLVAAIKSAMLQ
                     VPTLDPKAIEDAIIGCSFPEGEQGMNMARIAVGLAFDHPVGGVTVNRFCASGVTAIQM
                     AADRIRVGEADVMIAGGAESMSMVPMGGGKPSFNPAIFERDENVGIAYGMGLTAEKVA
                     QQWKVSREMQDAFALESHLRAIKAQQAGEFSDEITPIDVIERTPNLATGEVTTRTRTV
                     SLDEGPRPDTTLEGLAKLRTVFAARGSVTAGNSSQTSDGAGALILASEKAVKQFGLTP
                     LARFVSYAARGVPPEIMGIGPIEAIPAALRYAGINSQDIDWYELNEAFAAQSLAVVNT
                     LGLDPSKVNPMGGAIALGHPLGATGAIRAATVVHALRRHNLKYGMVTMCVGTGQGAAG
                     IFERV"
     gene            complement(9642..12041)
                     /locus_tag="PKX82_01205"
     CDS             complement(9642..12041)
                     /locus_tag="PKX82_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOF50891.1"
                     /translation="MSRFNVRKVAVLGAGVMGAQIAAHLVNVKVPVVLFDLPAKEGPK
                     NGIVTRAVEGLKKLKPAPLGVADDAVLIQQANYEEHMDVLKGCDLIIEAIAERMDWKL
                     DLYKKIAPFIAPHAIVASNTSGLSITKLSEALPEEIKPRFCGIHFFNPPRYMALVELI
                     NTPTTQPQILDDLEAFVTSNLGKGVVRAKDSPNFIANRVGIAGMLATMKEVQNFGLTF
                     DVVDDLTGKKLGRASSGTFRTADVVGLDTMAHVIKTLQDTLSLETDPFYESFATPEVL
                     KTLLEMGNFGQKTKAGFFKKVGRDVMRFDLASKEYVPAGQKADEVYGRMLKKPAGERL
                     KLLRNAEGAQGQFLWAILRNSFHYAAVHLASIADNARDVDFCMRWGFGMKQGPFELWQ
                     EAGWLEVANMIKEDIDAGKALCKTPLPDWVFKGPVADAGGVHTAAGSWNPTSGTFAPV
                     RKLPVYTRQHFPESVLGANAPSATTAGTTVQEDESMRLWTLDGDVLIASIKTKMHAIG
                     GGVIEGLNTAIALAEKEYQGLVIWSPDEMFSAGADLQSMLPGFMMGGVKAIDGAAHEL
                     QKVMLKLRYANVPTIAAIRGLALGGGCEMAVYSGKRVAAMESYIGLVEVGVGLVPGAG
                     GLTYIARRAAENMALSTSKDLLPFLTEGFTGAAMAKVGTSALESRKLGYLLESDAVVA
                     HKDELLFVAINEAKALFASGYRAPHKRLFPVAGRSGIATITAQLVNMRDGGFISAHDF
                     HISKLIAEVVCGGQVDAGTLVTEEYLMALERKAFCSLLTHPKTQERIMGMMSTGKPVR
                     N"
     gene            complement(12057..12491)
                     /locus_tag="PKX82_01210"
     CDS             complement(12057..12491)
                     /locus_tag="PKX82_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOF50892.1"
                     /translation="MIRSVLTFAIGISSFGAMAQMTPVGLWKTVDDSSGVVTSEIRIV
                     ENAGVVSGKIEKILDPAAKPGEKCVECNDERKDQPILGMEILRGLTKTEGKDVWEGGN
                     VLDPKNGKVYRATVKPLEGGAKLQMRGYIGFFYRTQTWIRVQ"
     gene            complement(12538..>13088)
                     /locus_tag="PKX82_01215"
     CDS             complement(12538..>13088)
                     /locus_tag="PKX82_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOF50893.1"
                     /translation="VFGGHGFIKEWGMEQFVRDNRINLIYEGTNTIQSLDLLGRKVLG
                     NNGATLRKFGKLVAKLVEEEGVNEKMAEFINPIAVLGDQMTKFTTEIGFKGFQNPDEV
                     GAAAVDYLRVAGHLVFGYFWARMAQVALRQIAAGNTDQFYVAKLQTARFYFAKLFPET
                     ATLMRTARAGSKVLMDTDAVFA"
BASE COUNT         2542 a         4121 c         3850 g         2575 t
ORIGIN      
        1 cttcatcaag gtcatggaca acctgaccat tgagcccgat ttttttggcg gtgccgtgca
       61 aacctttagc gccaccaagc gcctgggcag cgacgcctcc cgcctgtccc aaatccagga
      121 cggcaagtgg aagactgttt cggactactt tggcagcacc gccaagccgg ccgcggcacc
      181 cgcagccaag gcacccgcca aaaagtaatc ctgcctgctg gcgcgcaccg gaaggtgcgc
      241 gcttccttct ttgagttctc tagtttgtac ggagttgcgc catgaagaag ttgcttgcac
      301 tgtgtttccc cctggtcctg tcggcatccc ttgcccatgc ccagcagggt gtgactaaag
      361 atgaaatcct catcgggtcc attcaggatc tttccggccc gattgccgga ttcggcaaac
      421 aggcgcgctt cggaatgcaa ctggccgtgg atgaaatcaa tgagctgggg ggcatcaacg
      481 gccgcaagct gcgtgtgatc ttcgaggact ccggctacga ccccaagaag gccgttttgg
      541 cggcccagaa actggtcaac caggacaaga tcttcatgat ggtgggccat ctgggcacgg
      601 cccagaacct ggccacgatg ccggtgctgt ttgagaaaaa catcgtcaac ttcatgccca
      661 tcacgtcgtc caaggaaatg catgagccat tcaaccggct caagtacgcg ttcggaccca
      721 gctatttcga ccagatgcgc acctcggccc ccaaactggt caaggaaaaa ggagccaaaa
      781 aggtctgcac gctgtatcag gacgatgaat ttggactgga ggtcatgcgt ggcgcagaag
      841 ccggactgaa aaccatcgcc atggagttca cggaaaagac cacgtacaag cgtggcgcga
      901 ccgacttttc atcgcaggtt gccaaactac agtcttccgg atgtgatttt gtggtcctgg
      961 gcaccatcgt ccgggagacc atcggcacca ttgccacggc acgcaagatg ggttacagcc
     1021 ccaccttcct gggctccaac gcggcctaca ccgaactggt gcaccggctg ggcggcccgg
     1081 ccatgaacgg gttttatgcc acgaacacgg tgcagacacc ctacctcgac gatgcatcgc
     1141 aacccatccg cttctgggcc accaagtaca agaccaagtt caacgaagac ccgaccgtac
     1201 tttcgatcta cggctacctt ttcgtggaca cctttgccaa ggcagccacc aaggcggggt
     1261 ccaaactgag cacggaatcg ttcatcaagg cgatggacac catgaccatc ccgcccgata
     1321 tttttggcgg cacgcaaagc acgttctcgc ccaccaagca ctttgggacc gagacatcgc
     1381 gcatgtccca aatccaggat ggaaaatgga aggtggtatc cgactacccc aacgcgccag
     1441 catccagcgg cacgaagaag taggtttctt caagacacga tataggcgcc agcacctgtg
     1501 gacagcagct tgccgtccgc gcccagaaac tccatgcgcg tagaggctac gcgcgacccc
     1561 aggcgcatga cttcggcaca cagctcgaaa cgctcaccga tgcccgggcg caggtagtcc
     1621 acgcgcaggt caatcgttcc cagttttgaa aaacggtgca gacgctgcat gggtggctca
     1681 tccatgtggc gggcaccaat ggccgccatc acagccagtc cgcccatggc atccaaaccc
     1741 gcgctgatca cgccgccgtg aatccggttg aagctgtagt gcccgaccag ctgcggttgc
     1801 atgtcaatgt gggccaccac acgctctggc gtgatggcat cgatcttcaa ccccaacacc
     1861 ttgttgaagg tgatggattc ttcaaaaatc cgggtcaacc ctttgacgaa ctcctcctcg
     1921 aagacgacgg gttgaccgga cagggggttg gcactggttt taggcatgcc ggattgttac
     1981 agacccaact gacgggaagc caactccttc atgatctcct cggcgccgcc gccgatcatc
     2041 atgaccttaa cctcgcggta cacccgctcg caggtggtgc cacgcatgaa gcccatgcca
     2101 cccaggatct gcaccccctg gtcggcgcag aactgcatgg tctgcgtggc gtgatttttc
     2161 aacaggcaca cttgcgcaac ccattccgcg cccttggccg atttgtcgcc gcggtcgcca
     2221 gcgtcagcac gggcagaaac cgcattgagc caggcgcggg tggactcgat gcgcatcttc
     2281 atgtccatga acttgtggcg aatcacctgg tggtcgatca gcgcggtgcc aaaggtcttg
     2341 cgctgctggg cccagctcag ggcttcgtca tagcaggcct cggcaaagcc cagcgacagc
     2401 gcagccatcg acaggcgctc gccgttgaag ttggtcagga tcatcttgaa ccccgaaccc
     2461 tcttcaccca ccagattact caccggcaca cgcacgccgt caaaccggat ctgcgctgtg
     2521 tccgaacaca accagcccat cttcttcaaa gggctgcggg acaagccgac agcgtcgccc
     2581 ggaaccatca ccatggagat gcccatgggg cccttgtttt tcagatcggt gcgtacggcc
     2641 accgtcagcc agtcggcgcg cacacccgag gtgatgaaga ctttttcgcc gtcgatcaca
     2701 tactcatcgc cttcgcggcg cgcggtggtc ttgagtgccg acacatcggt gccgccgccc
     2761 ggttcggtga tggccagcgc agcaatcttc ttgcccgcga gcacatcggg aatgaccttg
     2821 cgctgcaatt ccggcgaccc atggcgcacc acgggtggca agccaatgtt gtggctgaac
     2881 agactggcca tcaagccacc actgccgcct gcacgtgcca gcgcgatcgt cagtgcattg
     2941 cgcagcttcc acggactggg tgtgccccca aactcttcgg ggtagcccat ttccagccag
     3001 cccatctccg cagcgcgctg gtacaggcta cgcggaatct ccccggcctc ttcccaggcg
     3061 cccagatggg gtgctacctc ggtctggaca aatttgcgcg cagcgtcttc gaccagggca
     3121 atatcttcat cggctatttc gtacacggac atgacttctc tctcaaaaaa caaaatggtt
     3181 cagctgcggg tgggccgctt ggccacgccc atggttttgg cgatgatgcc catcatgact
     3241 tcgtcggcgc caccaccaat ggagcccagg cggctgtcgc ggaacatgcg ggaaaccttg
     3301 ttctcccagg tgaagcccat gccaccccag aactgcaggc agccatcggg taccaggcga
     3361 cccagacggc ctgccttgag cttggccatg gaggccagct ccagcacgtc ctgcccggcc
     3421 acgtacaact cacaggcccg gtaggtcagc gcgcgcaggc tctcgacctc ggtcttcatc
     3481 tcggccaggc ggaactgcac ccactgctgg tccgccaacg tgccaccaaa catcttgcga
     3541 tcctgcgccc actccacagt ccattcaatg cagttgttga gcgcctgcaa ggcactggca
     3601 gcacaccaga ggcgctcctc ctggaactgc tgcatctggt agacaaagcc cgcgccttcc
     3661 tggccaatca ggttgcgctg cggtacgcgc acttcgtcaa agtagagcag accggtatcg
     3721 ctggactgca tgccgatctt tttgatcttg cgcgccactt cgatgccctt gcgcaacttg
     3781 ccgttctcgc gcagcggcac catgaccagg ctcttgttct tgtgggtcgg gccatcgccc
     3841 gtattgacca gcatgcacat ccagtcggcc tgcaggctgt tggtgatcca catcttctgg
     3901 ccggtgatca catagtcatc gccatccttg cgggccactg tcttgatgcc cgccacatcg
     3961 ctacccgcac ccggctcgct cacaccaata cagcccaccg tatcaccggc gatggcgggc
     4021 accagaaact cccgcttgag cgcatcgcta ccaaaacggg ccagcgccgg cgtgcacata
     4081 tcggtctgga ccccaatcgc catggggatg ccaccgcagt caatatggcc tagcgcctca
     4141 gccattgcca tgccatagct gtagtccagc cccgcaccac caaaggcctc gggcttggtc
     4201 aggcccagca atcccaggtt gcccagtttc ttgaagacct cgtgggctgg aaacatctcg
     4261 gcctcctccc actcctcaac atgcggattg atctccgcat cgatgaagcg cttgagagtc
     4321 ttctggattt ccaggtgttc gtgggtaaat tgcatgacac gcctcagact tgaaagacct
     4381 tgggcaaatg ggcacggtga aagccgttga aaggcttgac tgcagcgcgc tcttgggtcg
     4441 cagcatcagg cacgcgcatg ggatacccca tacgggcctg cggccgcgca ccatcgatac
     4501 gcaaggtggt accgctgaca aaggatgctg ccgggctcag caggaacacg atgccggcgg
     4561 cggtttcggc ttcggtacca aagcgcccag ccggaatggt cttggccatt tcacggatca
     4621 tgggagccat ctctacggga tagttgtcca tgccgctcga tgcgatgtag cctggcgcaa
     4681 cggcgttgac acgcaccccg cggccggccc attccaccgc ggcggtttcg gtgaagctga
     4741 ccatgccggc gcgtgctgcg ccgctgtggc ccatgccggg catggaaccc cacatgtcgg
     4801 caacgatgtt gacaatgctg ccaccgttct tgctcatgct ctgcaggtag cactcgcgcg
     4861 ccatcaggaa gcccccggtg aggttggtat tgaccaccgc ctcccagccc ttggcactga
     4921 tagcctccag gggggtcatg aactgacccc cagcgttgtt gaccaggcca tcgacacggc
     4981 catgctcggc aacgatgtcc gctacggttt tcttgacaga ctcttcctgg cgaatgtcgc
     5041 aggcggccat tgttgcccgg ccaccgtcct gcgtaatctc ttcctgtacc ttctgcagtt
     5101 tctccagctt gcggcccacc agcaccacgg tggcgcccaa agccgccaac tcgtgcgccg
     5161 tgcaacgacc gatacccgac ccgccccccg tgacaaccat caccttgtcg gcgaacaaac
     5221 ctggggcaaa aactgaaccg taagacatca ggactccttg ataaacatgg aaatggcagc
     5281 gtcggtcagg gtgtcgagcg acgcgccttt tttgggatca aaccattgca cggcccagtt
     5341 cagggcaccg agaatcagca gccgcgccag cggcgccggc gcacgcagct ggccgctttg
     5401 ctccagcgac accagcaccg gcatccacag ggattcgtat tgggcgatca gcttggccag
     5461 cgacttctgc tgacgtgagt tgagtgaccg gttttcgtac agcatgaccg gaacaaagtc
     5521 attgcccggc cccagcagga cgtcaaagtg cgtacgcacc agtgcgcgca agcgttcgga
     5581 ggaaggcagg gtgttgtatt ccggggccgc aaccaccgcg cgcagaaagt tcagcgcagt
     5641 acccatgccc tcttccatca cggcatacag cagtgcgtcc ttgcttttga aatggtagaa
     5701 cggcgagccg ctttgcatgc cggcggctgc ggcaatgtcg cgtgtggtgg tggctgcaaa
     5761 gcccttgcgc cgaaagagct tggcggcggc ctgaagcagc gcgtggcggc ggttcccgtc
     5821 atcgcgctcg tccaccgtct tgcgggggcg cccgcgcggt cgcttgggaa tttcgggcgc
     5881 ggaaatggct tggtctggca tgggagccag acgataccaa actaatttat ttttagcaag
     5941 cgtttgcttg gtaaaaataa tcgcttattt atttttgagc tttccgcgag ggatcactgc
     6001 cgtggtgatc gtggcgcgca cgggctccga cagacccgac ggcggcttgg gcgcagaggt
     6061 gtccttcttg ggtttcttgg cttccttgtt ggttttttgt tgacctttgg ccatagcgaa
     6121 ctcctttgat tggtgtgtct gcagtttaat acgcccagta tgacttcccc cattgccatc
     6181 gccgaaaacg aagtgcaggt caccgcggtg cgtgcacagg gcgccggcgg gcaaaacgtc
     6241 aacaaggtgt ccagcgcgat ccatttgcgt tttgacattc ccgcctcgtc cttgccgcag
     6301 ggcgtgaaag accggctgct ggccttgcgc gacagccgca tcacgcaggc gggcgtcctg
     6361 gtgctcaaag cccagcagca ccggacccag gacatgaacc ggctcgatgc atttgcacgc
     6421 ctgcatgagc tggtcaacag cgtggccacg ccaccccgca ttcgccacgc aacgaaaccc
     6481 acacgcgcct cgaaacagcg acgcctggaa gccaagaccc agcgctcgga aatcaaggca
     6541 gggcgcggga aaatttcaga ttagcgaatg tttgctatta tttttatagc taactacgca
     6601 atgaatacgg gggcaaggcc ccgaattcat ataaaaacct cagaccttgc tgaagtccgg
     6661 cttgcgcttt tggcgaaagc gccagcctgc tgcgccggcc ctgcgggctc gaccttcaga
     6721 cgttgctgaa gtccggcttg cgcttttggc gaaagcgcca gcccgctgcg ccggccctgc
     6781 gggctcgacc ttcagacttt gctgaaatcc ggcttgcgct tttccatgaa ggcggtgaag
     6841 gcttcgcgtg cggccggctc gcgcagcatg cgtccaaagc tcgcgccctc ttctgccatc
     6901 tgggccagca ccaggctggt ttggcctttc ttcatgaagc gcttggtttc caccagcgac
     6961 gacagcggct tggccgcgag cttgcgtgcc tgcgcctgcg ctacggcgtt ggcttctgtc
     7021 gggggtacca cacggttgac cagccccacc tccatggccg cctcggccat gaacggctcg
     7081 cccagcagca gagcctcagc ggcccggtgg taccccatca tctgcggcac cagcaagctg
     7141 gaggccgcct cggggcacag gcccaggttg acgaagggca tggaaaaggc cgcgttgtct
     7201 ccggcgtaca ccaggtcgca atggaacagc atggtggtgc ccacaccgac cgccgggccg
     7261 cagactgcag cgaccagcgg cttcgggaac tgggcaatgc cctgcaggaa ccggtacacc
     7321 ggtgactcca gtgtggacgt cgggccgttg agaaaatcgc caatgtcgtt gcccgcactg
     7381 aagatggtct cgtgcccttg caggacgaca gcgcgcacgg ctgcatcagc tgcagcctgg
     7441 ttcaacgcat cggccatggc gccatacatg gcctgggtca gcgagttctt cttgtccagg
     7501 cggttgatgg tcagtgtcat cacgccggca gcggtgtcga tcaggatatc ggacatggtg
     7561 ggttctccta ttctttgtag taaacgatct ggtgggtggt gacgagcagg atgccggctt
     7621 cgttccacag ctggctggtc tggtcgaagt agccgttgcg aaaggcctgc gcccgggcct
     7681 gccccagcag gtagcccgta cccgtctgcg cgagctgttc ggtgctggca tggaaataca
     7741 ccgtcatgga aacggtgccc acaggaacca gccgggcgcg gcgaagaaat acgcgtggaa
     7801 agaaaagatc cgacagcgcc gtgagcgacg caaagtccag cggacgcggc gggttgtcgc
     7861 gcatccacag ctgcgacagg ctgctgtcac cgccgtcatt ccagactccc ggcaatgttc
     7921 cggtcagggc gcgcatttca tagcgctggg cccacgccag aggaggcgat tgtgccggca
     7981 gcggaacctg atcaggcgcc gggcagaccg gcataggctc ctcatccaca ctccaggtct
     8041 cgcgccgcgc agccgtgacg gccgtggcgg tgatcaccgt ctgcccctgc tggcgcatct
     8101 cgatgaccca gtgctgggtg gaacggttgg tgcgggccgg gcgcgcatgc acctcaaagg
     8161 caccatcggc aacgcctgtg cagtagttga cggtcagtgc cactggttca cccaaacgct
     8221 gcggatgctg caacaccgcc gacagcactt gcgctgcggt aatgccgcca aaaggcccca
     8281 ccatgttggc ataggcagga tgggtttgcc cggcccacaa gccgtcgcct tgcgacgaca
     8341 gtgcgatggc ctggtcaaaa acgtgttcag tcatgcaatg agatccatgc aaaaggccac
     8401 cttgcggtgg ccttggttgc gattgggttt acacccgctc gaaaattccg gccgccccct
     8461 ggccggtacc cacgcacatg gtcaccatgc cgtacttcag attatggcga cgcagggcgt
     8521 gcaccactgt cgccgcccgg atagcaccgg tggcgcccaa cgggtggccc aaggcgatgg
     8581 cgccgcccat ggggttgacc ttggatgggt ccagtcccag ggtgttgacc accgccagcg
     8641 actgcgcggc aaaggcttca ttgagctcgt accagtcgat gtcctggctg ttgataccgg
     8701 cataacgcag ggcggcggga atcgcctcga tcgggccgat gcccatgatc tcgggcggca
     8761 caccgcgtgc agcgtaggac acaaagcgcg ccaacggcgt caggccaaac tgcttgacgg
     8821 ctttttcgct ggccaggatc agcgcaccgg cgccgtcgct ggtttgagag ctgttaccag
     8881 cggtcacact gccgcgcgcg gcaaacacgg tgcgcagctt ggccaggcct tcgagcgtgg
     8941 tgtcggggcg cgggccttcg tccaggctca cggtgcgggt gcgagtggtg acttcgccgg
     9001 tggccaaatt gggcgtgcgc tcgatgacgt cgatgggagt gatttcgtcg ctgaattcac
     9061 cggcctgctg cgccttgatg gcgcgcaggt gcgactccag ggcaaaagcg tcctgcatct
     9121 cgcgcgagac cttccactgc tgcgccacct tctcggccgt caggcccatg ccgtaggcaa
     9181 tgccgacgtt ttcatcgcgc tcgaaaatcg cggggttgaa ggagggcttg ccgccaccca
     9241 tgggcaccat gctcatggac tcggcaccac cggcgatcat cacgtcggcc tcgccgacac
     9301 ggatgcggtc ggcggccatc tggatggcgg tcacaccgga ggcgcagaag cggttgacgg
     9361 tgacgccgcc cacggggtgg tcaaacgcca gacccaccgc gatgcgggcc atgttcatgc
     9421 cctgctcgcc ttcggggaaa gagcagccga tgatggcgtc ctcaatggcc ttggggtcca
     9481 gcgtgggcac ctgcagcatg gcgctcttga tggcggccac cagcagttcg tcggggcggg
     9541 tgttgcggaa aaaaccacga ccagagcggc cgataggggt gcgggtggcg gcaacgatgt
     9601 aggcgtcttg tacttgtttc atagatttct ttccttgccg attagttacg cacaggcttg
     9661 ccggtggaca tcatgcccat gatgcgttcc tgggtcttgg gatgggtgag caggctgcag
     9721 aacgctttgc gctccagggc catgaggtat tcctctgtga ccagggtgcc ggcatccacc
     9781 tggccaccac agaccacctc ggcgatcagc ttggagatgt ggaagtcgtg cgcgctgatg
     9841 aagccgccat cacgcatgtt gaccaactgt gccgtgatgg tggctatgcc actgcggccc
     9901 gccaccggga acaggcgctt gtgcggggca cggtaaccgc tggcaaacag ggccttggct
     9961 tcgttgatgg ccacaaacag caactcatcc ttgtgcgcca cgacggcgtc gctctccagc
    10021 aggtaaccga gcttgcggct ctccagcgcg ctggtgccca ccttggccat ggcggcgccg
    10081 gtgaaacctt cggtcaggaa aggcagcagg tccttggacg ttgacagcgc catgttttcc
    10141 gccgcacggc gggcgatgta ggtcaggccg ccagcaccgg gcaccaggcc cacgccgact
    10201 tcgaccaggc cgatatagct ttccatggca gcgacgcgct tgccagagta gaccgccatt
    10261 tcgcagccac cgcccagtgc cagaccgcgg atcgcggcga tggtgggcac gttggcatag
    10321 cgcagcttga gcatcacttt ttgcagttcg tgtgcagcgc cgtcaatagc cttgaccccc
    10381 cccatcatga agccgggcag catggactgc agatcggcgc cggcggagaa catctcgtcg
    10441 ggcgaccaga tcaccagacc ctggtactct ttctcggcca gtgcaatcgc cgtgttcagg
    10501 ccctcaatga caccgccacc aatggcgtgc atcttggtct tgatgctggc aatgagcacg
    10561 tcaccatcga gcgtccacag acgcatggac tcatcttcct gcaccgtcgt gccggccgta
    10621 gtggcggaag gcgcgttggc acccagcaca ctctcgggaa agtgctggcg tgtgtacacc
    10681 ggcagcttgc gcaccggggc aaacgtgccc gatgtggggt tccaggaacc tgcagcggtg
    10741 tgcacaccgc cagcgtcggc caccgggcct ttgaagaccc agtcgggcag cggtgtcttg
    10801 cacagcgcct tgcccgcgtc gatatcttcc ttgatcatgt tggccacttc gagccagccg
    10861 gcttcctgcc agagctcgaa cggaccctgc ttcatgccaa agccccagcg catgcagaag
    10921 tccacatcgc gggcgttgtc ggcaatggaa gccaggtgca cggcggcgta gtggaaactg
    10981 ttgcgcagga tggcccagag gaactggccc tgtgcacctt cggcattgcg caacagcttg
    11041 aggcgctcgc cagcaggctt cttgagcatg cggccataca cttcgtcggc cttctggcct
    11101 gcgggcacgt attctttgct tgccaggtca aagcgcatca catcgcggcc aaccttcttg
    11161 aagaaacccg ctttggtctt ctgcccgaaa ttgcccatct ccagcagggt cttgagcacc
    11221 tcaggcgttg caaagctctc gtagaaagga tcggtctcca ggctcagcgt gtcctgcagc
    11281 gtcttgatca cgtgggccat ggtgtccaga ccgaccacat ccgccgtgcg gaaagtgccg
    11341 gagctggcgc ggcccagctt cttgccggtg aggtcatcga ccacatcaaa ggtcaggccg
    11401 aagttctgga cttccttcat ggtggccagc atgccggcga tgccgacgcg attggcgatg
    11461 aaattgggag agtccttggc acgtaccacg cccttgccca ggttgctggt gacaaaggct
    11521 tccagatcgt ccaggatttg tggctgggta gtgggggtgt tgatcaactc caccagcgcc
    11581 atgtagcggg gtgggttgaa gaaatggatg ccgcagaaac gcggcttgat ttcctcgggc
    11641 aatgcttcgg acagcttggt gatcgacagg cccgaggtgt tggatgccac gatggcatgg
    11701 ggagcgatga acggcgcgat cttcttgtac agatcgagct tccagtccat gcgctcggca
    11761 atcgcttcaa tgatgaggtc gcaacccttg agcacgtcca tgtgctcttc gtaattggcc
    11821 tgctggatca gcacggcgtc gtccgccaca cccagcgggg cgggcttgag tttcttcaag
    11881 ccctccactg cacgcgtcac gatcccgttt ttggggcctt ccttggctgg caggtcaaac
    11941 aagaccaccg gcactttgac attcaccaga tgggcagcga tttgcgcacc catcacccct
    12001 gcgccgagga cggcgacttt acgaacattg aaacgggaca tgaggtttcc tgaaaattac
    12061 tggacgcgga tccaggtttg ggtccggtag aagaatccga tgtagccgcg catctgcagc
    12121 ttggctccac cttccaatgg cttgactgtg gcgcggtaca ccttgccgtt cttgggatca
    12181 aggacgttgc cgccctccca cacatcttta ccttccgtct tcgtgagtcc acgcaagatt
    12241 tccatgccca gaatgggctg gtccttgcgc tcatcgttgc attcgacgca cttctcgcca
    12301 ggtttggctg cgggatcgag gattttctcg atcttgccgg ataccacccc ggcgttctcg
    12361 acgatgcgga tttcagacgt aactacaccg ctactgtcgt caacggtttt ccacaaaccc
    12421 acaggcgtca tctgtgccat agcaccgaag gacgaaatac cgatagcaaa ggtcagaacg
    12481 gaacgaatca tggctgtctc ctctttaata ttgttggcca atcgttgata aatcgcttta
    12541 tgcgaatacc gcgtccgtat ccatcagcac cttgcttcca gcacgggccg tacgcatgag
    12601 cgtagcggtt tccgggaaga gcttggcgaa atagaaacgt gcggtctgca gtttggcgac
    12661 atagaactgg tcggtatttc cagcggcgat ctggcgcagt gcgacctgtg ccatacgtgc
    12721 ccagaaataa ccgaatacca ggtggccagc cacgcgcaga tagtccacgg cagcggcgcc
    12781 cacttcgtca gggttctgga aacccttgaa tccgatttcg gtggtgaact tggtcatctg
    12841 gtcaccgagc acagcgatcg ggttgatgaa ctcggccatc ttctcgttga cgccctcttc
    12901 ttccaccagc ttggccacca gcttgccgaa cttgcgcaat gtggcaccgt tgttgcccag
    12961 caccttgcgg cccagcaggt ccagggactg gatggtgttg gtgccttcgt aaatcaggtt
    13021 gatgcggttg tcacgcacaa actgctccat gccccattcc ttgatgaagc catggccgcc
    13081 gaagacct
//
LOCUS       DAOSJM010000016        12887 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_4909_length_12887_cov_4.273691, whole genome shotgun sequence.
ACCESSION   DAOSJM010000016 DAOSJM010000000
VERSION     DAOSJM010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4909_length_12887_cov_4.273691"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PKX82_01220"
     CDS             complement(<1..1168)
                     /locus_tag="PKX82_01220"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOF50894.1"
                     /translation="MHDILEQLEAKRELARMGGGQKRIDAQHKKGKLTARERLEILLD
                     EGTFEEWDMFVEHRCVDFGMADQKIPGDGVVTGYGMINGRLVFVFSQDFTVFGGALSE
                     AHAEKICKVMDQAMKVGAPVIGLNDSGGARIQEGVASLGGYADVFQRNVMASGVVPQI
                     SMIMGPSAGGAVYSPAMTDFIFMVKDSSYMFVTGPEVVKTVTHEEVTAEELGGAITHT
                     TKSGVADLAFENDVEALLMLRRLYNYLPLSNREKAPVRKSGDPIDRMDLSLDTLVPDN
                     PNKPYDMKELIVKTVDDGDFFELQPEYAKNILIGFARMDGQTVGIVANQPLVLAGCLD
                     IKSSIKAARFVRFCDAFNIPVVTFVDVPGFMPGTSQEYGGIIKHGAKLLYAYAEC"
     gene            complement(1204..2226)
                     /gene="meaB"
                     /locus_tag="PKX82_01225"
     CDS             complement(1204..2226)
                     /gene="meaB"
                     /locus_tag="PKX82_01225"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOF50895.1"
                     /translation="MLDSLLNGSAMVQRRAIAKAITLLESTRVDHRAQGDALLTALLP
                     HTGKSFRLGISGVPGVGKSTFIEALGLYLIGQGHRVAVLTIDPSSSVSGGSILGDKTR
                     MEHLSVHEKAYIRPSPSSGTLGGVAEKTREAMLVCEAAGYDIVIIETVGVGQSETAVQ
                     GMTDMFVLMQLPNAGDDLQAIKKGVMELADLVVINKADIDANAAMRAQAQITSSLRLL
                     GMHGNPNDPHSDKHWQPQVISLSALQGQGVDRFWAAVSQFQALQKANGLFDSRRQHQS
                     LAWMWERIDAGLKQAFKQDPAVRALLPTLTAAVQAGQIAASTAARNLLAAPVNTAQAA
                     MNSVAI"
     gene            complement(2219..2827)
                     /locus_tag="PKX82_01230"
     CDS             complement(2219..2827)
                     /locus_tag="PKX82_01230"
                     /inference="COORDINATES: protein motif:HMM:NF036878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOF50896.1"
                     /translation="MRTQVILVDWENVQPELLPALDVDETKLVVFIGPHQTKLPFAMV
                     EAVQKFGDRAEYVKVSKQGKDALDMHIAFHIGRMSTQLDDAYFHVISKDGDYDPLIAH
                     LKEKFGIFAARWSTLNAIPVIRRAQAKTPKDQLEVTREWLIERKAGRPKTLKTLSNSL
                     KTSVFVGRLTDEEIESLLVALQANNWIKILGQKVSYAEALDA"
     gene            complement(2832..5003)
                     /gene="scpA"
                     /locus_tag="PKX82_01235"
     CDS             complement(2832..5003)
                     /gene="scpA"
                     /locus_tag="PKX82_01235"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOF50897.1"
                     /translation="MTDKTPEFKSATLDAWAKAAAKSAPGGNVDALNWITPDGIAVKP
                     LYTAADTASLQYADTLPGFEPYLRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKAL
                     AAGGQGVSVAFDLATHRGYDSDHPRVTGDVGKAGVAIDSVEDMKILFDQIPLDKVSVS
                     MTMNGAVLPVLAGYVVAAEEQGVSQDKLSGTIQNDILKEFMVRNTYIYPPAPSMRIIG
                     DIIGYTAKNMPKFNSISISGYHMQEAGANQALELAFTLADGKEYVKTAIATGLDVDEF
                     AGRLSFFWAIGMNFYLEVAKMRAARLLWCRIMKGFDAKSPKSLMLRTHCQTSGWSLTE
                     QDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSSRIARNTQLIIQEETHI
                     TNVIDPWAGSYMMEKLTQDMADAAWKIIEEVEAMGGMTKAVDSGWAKLKIEAAAADKQ
                     ARIDSGQDVIVGVNKYKLKSEDVVEARDIDNVAVRESQVARLKEIRSKRDAALVDAAL
                     AAITSAAESGTGNLLDLSIQAVRLRATVGEVSDALEKAFGRHRADTNKVSGVYAAAYD
                     AAQGDTMEYWNTLKADIAAFADKQGRRPRVMISKLGQDGHDRGAKVVATAFADLGFDV
                     DMGPLFQTPEECARQAIENDVHAVGVSTLAAGHKTLVPAIIAELRKQGADDIIVFVGG
                     VIPRQDYDFLYAAGVKGIYGPGTPIPVSARDVLSQIEKALG"
     gene            5147..5818
                     /locus_tag="PKX82_01240"
     CDS             5147..5818
                     /locus_tag="PKX82_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOF50898.1"
                     /translation="MSALMSEIRPPASLSPRALYEEVAELLRQRIFRRELEPGSWIDE
                     LKIAEDYGISRTPMREALKVLAAEGLVTMKVRRGAYVTEVNEKDLTDVYHLLSLLESD
                     AAGVVAVRATDAQLRELQTLHDELETAACPGKQDRERFFEINEHFHMRLLEIADNRWR
                     DQMVADLRKVMKLNRHHSLFKSGRIEESLQEHRAIMAALLARDPVATMETMRAHFSNG
                     LEAAV"
     gene            5929..8502
                     /locus_tag="PKX82_01245"
     CDS             5929..8502
                     /locus_tag="PKX82_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF50899.1"
                     /translation="MTDNNSPTPSADAVKQTLLEYQAILENASVGILFSRGRKVLHCN
                     PRFSEIFGWEQGELVGQPATVLYFSPEDYSELGRLAGPVLARGELLDIERVMRRKDGS
                     GVHCHLNSRAINPQNTAEGTIWIVEDIGERKRAETEMKDLMLKQQAILENASLGIMFT
                     SNGHIVHCNPRVEAILNWPQGTLEGQKAGVFFSSMEDYALFGKQVGPQLARGELVDVS
                     WLNQCKDGSTIWCRHLAKALTTTDGSKSTIWITEDISEKRAAQEALQKAHQELEQRVE
                     QRTEELAAANTRLLSEIAERKQIENNLRASEARFRDLSELSSDWFWEQDADFRFVELM
                     TGPRYTQLPPGDTYGKTRWELPILGVTPAQWEQHRALLAAHLPYREFIYQLELPDGER
                     HWLSVSGKPIFDNGVFMGYRGTGTDITQRKATEQQIEYLAYHDPLTGLPNRMLLEDRM
                     QQSLAHAERNHFRLALLFLDLDNFKKINDSLGHATGDILLKEIASRLGECVRDTDTIS
                     RQGGDEFVLILNDLPDPDACLPVLVKILNRLQDPFMADGNELSTSVSIGIAVYPEDGR
                     DFETLRKKADMAMYRAKEAGRNTYHFFDDAMNDEAVEHLQMRNGLRRALERSELVLYY
                     QPQIDLASGRVVGTEALLRWNHPDFGLVAPGRFIPIAEDSGLIVPIGEWVITEACRQA
                     VAWQKAGLPALQMAVNLSAVQFKRGNVEQIVITALQQSGLAPSLLELELTESILIENV
                     EQVLSTVKRLKQLGVKLSIDDFGTGYSSLSYLKRFDIDKLKIDQSFIRDLATDTDDAA
                     IVNAVIQMARSLNLKTIAEGVEHPDMLDLLRGFQCDEVQGYYFARPMPADEFSAYLSG
                     N"
     gene            complement(8499..9494)
                     /gene="dusA"
                     /locus_tag="PKX82_01250"
     CDS             complement(8499..9494)
                     /gene="dusA"
                     /locus_tag="PKX82_01250"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HOF50900.1"
                     /translation="MRDLKWNNVAAELNPWRMSVAPMMDWTDRHCRYFHRLLSRHALL
                     YTEMVTTGALLHGDVPRHLRFNVEEHPLALQLGGSEPADLAHCARLGQDWGYDEININ
                     CGCPSERVQRGAFGACLMHEAPLVAECVKAMVDVVDVPVTVKHRIGIDRDESYGFVRD
                     FVGTVADAGCKVFIVHARNAWLKGLSPKENREIPPLRYAVVHQLKQDFPHLTIAINGG
                     FITSEQVHQELPHVDGVMVGREAYHNPWWLSRWDAEFYGSAVVDMSRESVEEQMVQYM
                     EREAQAHGTPWYAIARHMLGLRHGLPGARRWRQVWSDHRLKGLPAREVWTQAGAC"
     gene            complement(9482..>9640)
                     /locus_tag="PKX82_01255"
     CDS             complement(9482..>9640)
                     /locus_tag="PKX82_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOF50901.1"
                     /translation="MTAAPWLWLKLRGHSPDSGAWGVRIAGAALAASSAWALWMGLVH
                     NTAPWCAT"
     assembly_gap    9641..9650
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9651..10164)
                     /locus_tag="PKX82_01260"
     CDS             complement(<9651..10164)
                     /locus_tag="PKX82_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOF50902.1"
                     /translation="MQSSLALTALLMGLVGGPHCVAMCGAACAGIGQAAGPNKNSAIL
                     TFQVGRIAGYSLLGGLAAASMQGLGWLTVQSAALRPVWTLFHVAAALLGLVLMVRARQ
                     PVWLESVGRKVWAKARGWGGQSGGAPLVLGVLWALLPCGLLYSALLVAAMAGGPLDRA
                     LVMALFAQGTS"
     gene            complement(10174..11568)
                     /gene="hemN"
                     /locus_tag="PKX82_01265"
     CDS             complement(10174..11568)
                     /gene="hemN"
                     /locus_tag="PKX82_01265"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOF50903.1"
                     /translation="MSAEVETVTDDLLRRYDVSGPRYTSYPTADRFVEAFTAEDYAQA
                     LTQRRSGAAALALPLSLYVHIPFCESLCYYCACNKIITKHHDKAAEYLRYLSREVDLH
                     TQQMGTGQVVSQLHLGGGTPTFLSDDELRELMAMLRRNFSFAPGGEYSIEVDPRTVDA
                     GRLDTLAELGFNRLSFGVQDFDPAVQKAVHRVQPAEQVFALVAAARARGFESINVDLI
                     YGLPKQSPESFDRTLAQVAQLRPDRIALYAYAHLPERFKPQRRIAIVELPTGGSKISM
                     LSRSLTAFMSAGYVYVGMDHFALPDDALAVAKRQGRLHRNFQGYSTQPDCDLIALGVS
                     SIGRIGATYSQNAKTLEEYYDALDNGRFPVVRGLALSRDDLVRRAVIMALMCQGQLLF
                     ESINLSYLIDFSAYFAKELEALQVLQEQGMVVVDAHGIQVTAQGWFFVRAVAMVFDRY
                     LQTDRTRAKFSKII"
     gene            11769..12488
                     /gene="fnr"
                     /locus_tag="PKX82_01270"
     CDS             11769..12488
                     /gene="fnr"
                     /locus_tag="PKX82_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HOF50904.1"
                     /translation="MHPQAIKVACSNCNLRELCMPLGLSPVELDRIDDVVASRRKIKR
                     GATLFRNGEKFHNLYAIRTGFFKTCVASEDGRDQVTGFQMAGEIVGLDGIVNEHHTCD
                     AVALEDAEVCVMPFDRIEELSREVNALQRHVHKIMSREIVRENGVMLLLGSMRAEERL
                     AAFLLNLVQRLHARGFSQSELVLRMTREEIGSYLGLKLETVSRTFSKFVEDGIVEVKQ
                     RHVRILNTEALKEIVNPKVCD"
     gene            complement(12545..12817)
                     /locus_tag="PKX82_01275"
     CDS             complement(12545..12817)
                     /locus_tag="PKX82_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HOF50905.1"
                     /translation="MAQSEPTSPRPWWRFGHVWLVISGPAIVVVASFITFYLAANGTD
                     HVVDEDYYRKGIEINRTLADTPESLAPAVQARNHAQTGVKPSPKTP"
BASE COUNT         2776 a         3895 c         3618 g         2588 t
ORIGIN      
        1 tgcactcggc gtacgcatac agcagcttgg cgccgtgctt gatgatgccg ccgtactcct
       61 ggctggtacc aggcatgaag ccgggcacgt ccacaaacgt gacgacgggg atgttgaacg
      121 catcgcagaa gcgcacaaag cgcgcggcct tgatggagct cttgatgtcc aggcagccgg
      181 ccagcaccag cggctggttg gcgacgatac ccacggtctg gccgtccatg cgggcaaagc
      241 cgatcaggat gttcttggcg tactcgggct gcagctcgaa gaagtcgcca tcgtccaccg
      301 tcttgacgat cagctccttc atgtcgtaag gcttgttggg gttgtcgggc accagcgtgt
      361 ccagcgacag atccatacga tcgatcgggt cgccgctctt gcgcaccggt gctttttcgc
      421 gattggagag gggcaggtag ttgtagaggc ggcgcagcat caacagcgct tccacatcgt
      481 tctcaaacgc cagatcggcc acaccgctct tggtggtgtg ggtgatggcg ccgcccagct
      541 cttccgcagt gacttcctcg tgcgtcacgg tcttgaccac ttcggggccg gtcacgaaca
      601 tgtagctgga atccttcacc atgaagatga aatcggtcat ggccggggag taaaccgcgc
      661 caccggccga ggggcccatg atcatgctga tctggggcac cacgccgctg gccattacgt
      721 tgcgctggaa cacatcggca tagccgccca gcgaggccac accttcctgg atacgcgcgc
      781 cgcccgaatc gttcaggccg atgaccggag cacccacctt catggcctgg tccatgacct
      841 tgcaaatttt ctcggcatgg gcttcagaca atgcaccgcc aaacacggtg aagtcctggc
      901 tgaacacgaa caccaggcgg ccattgatca tgccgtaacc ggtgaccacg ccgtcgccgg
      961 ggatcttctg gtcggccatg ccgaagtcca cgcagcggtg ctccacaaac atatcccact
     1021 cttcgaaggt gccttcgtcg agcaggatct ccaggcgttc gcgggcggtg agcttgccct
     1081 tcttgtgttg ggcatcaatg cgcttctggc ccccacccat gcgggcgagt tcgcgcttgg
     1141 cttctagctg ttcaaggata tcgtgcatgg taggtccttc gttgtcgtct ctgatgtcaa
     1201 tggttaaatt gctactgaat tcatagctgc ttgcgcagta ttcacggggg ctgcaagcag
     1261 atttcgtgct gcagtgctgg ccgcaatctg tcctgcctgc actgcggcag tcaaggtggg
     1321 cagcagcgcg cgcaccgccg ggtcctgctt gaacgcctgc ttgaggccgg cgtcaatgcg
     1381 ttcccacatc caggccagcg actggtgctg gcggcggctg tcgaacaggc cattggcttt
     1441 ctgcagcgcc tggaactgcg ataccgcagc ccagaaacgg tccacgccct gcccctgcaa
     1501 ggcactcaat gaaataacct gaggttgcca atgtttgtcg ctgtgcgggt cgttcggatt
     1561 cccgtgcatg ccgagcaaac gcaggctcga agtgatctgg gcttgcgccc gcatggcggc
     1621 gttggcatcg atatcggcct tgttaatcac caccagatcg gccagctcca tcacgccctt
     1681 cttgatcgcc tgcagatcgt cgcccgcgtt gggcagttgc atcagcacaa acatgtcggt
     1741 catgccctgc actgccgtct cgctctgacc cacacccacg gtttcgataa tcacgatgtc
     1801 gtaaccggct gcttcgcaga ccagcatggc ctcgcgcgtc ttctcggcca caccacccaa
     1861 agtcccactg gacgggctgg ggcggatata ggctttctcg tgtaccgaca aatgctccat
     1921 gcgggtcttg tcccccagga tggagccacc ggatacggaa gacgagggat cgatggtcag
     1981 caccgccacg cggtggcctt gcccgatcag gtacaagccc agtgcctcga tgaaagtact
     2041 cttgccgacg cccggcacgc cactgatacc cagccggaag gattttccgg tgtgcggcaa
     2101 cagcgcagtc agcagcgcat cgccctgcgc acggtggtcc acgcgggtgg actccagcag
     2161 ggtaatggct ttggcgatgg cacggcgctg caccatcgca gagccgttga gcagggaatc
     2221 aagcatctag tgcttccgcg taggagacct tttgccccag aatctttatc caattgttcg
     2281 cctgcaaggc aaccaacaat gattcaattt cttcatctgt caagcgacca acgaacaccg
     2341 aagtcttcag actattgctc aaagtcttga gtgtttttgg gcggcctgcc ttgcgctcta
     2401 tcagccattc acgcgtgacc tcgagctggt ctttcggcgt cttcgcctga gcccttcgga
     2461 taacaggaat tgcatttagc gtcgaccaac gtgcagcaaa aattccaaac ttttctttta
     2521 gatgagcgat aagtgggtcg taatcgccat ctttcgaaat tacgtggaag taagcgtcgt
     2581 ctagctgagt agacattcgc ccaatgtgaa atgcaatatg catgtcaagc gcgtctttgc
     2641 cctgcttgct gaccttaacg tactctgctc gatcaccaaa cttttgaacg gcctccacca
     2701 tggcaaatgg gagttttgtc tggtgtggac cgataaaaac taccaacttg gtttcgtcca
     2761 catcgagtgc gggcagcaac tcaggttgca cattctccca gtcaacgagg atgacttgtg
     2821 ttctcatgaa gtcacccgag ggccttttca atctggctca gcacgtcgcg cgccgacacc
     2881 ggaatcggag tgccgggtcc gtaaatgcct ttcacccccg cggcgtacaa gaagtcgtag
     2941 tcctggcgcg gaatcacgcc gccgacgaag acgatgatgt cgtcagcgcc ctgctttctg
     3001 agctcggcaa tgatggcggg taccaaagtt ttgtggccag cagcgagggt actcacaccc
     3061 acagcgtgca cgtcgttttc aattgcctgg cgggcgcatt cttctggggt ctggaacagc
     3121 gggcccatgt ccacgtcaaa gcccagatcg gcaaacgccg tggccaccac tttggcgcca
     3181 cggtcgtgtc cgtcctggcc cagcttgctg atcatcacgc gcgggcggcg gccctgcttg
     3241 tcggcaaagg cggcaatgtc ggccttgagg gtgttccagt attccatggt gtctccttgg
     3301 gccgcatcgt acgctgcagc gtacacgccc gagaccttgt tggtgtcggc acggtggcgg
     3361 ccaaaggctt tttccagcgc atcgctcacc tcgcccaccg tggcgcgcag gcgcacggcc
     3421 tggatgctca ggtccagcag gttacccgta ccggattctg ctgcagaagt gatagcagct
     3481 agcgcagcat ccacgagggc cgcatcacgt tttgacctga tttccttgag gcgggcgacc
     3541 tgcgactcgc gcaccgccac gttgtcaatg tcgcgcgctt cgaccacgtc ttcgcttttg
     3601 agcttgtact tgttgacgcc cacgatcaca tcctgcccgc tgtcgatgcg cgcctgcttg
     3661 tcggcggcgg cagcttcgat cttcaacttg gcccagccgc tgtccacggc cttggtcatt
     3721 ccgcccatgg cttcgacttc ttcaatgatc ttccaggccg cgtcggccat gtcctgcgtc
     3781 agcttttcca tcatgtagct gccggcccag gggtcgatca cattggtaat gtgggtttct
     3841 tcctggatga tgagctgggt gttgcgcgca atgcggctgg agaactcggt gggcaacgcg
     3901 atggcttcgt ccagcgcatt ggtgtgcaga ctctgcgtgc cgccaaacac cgcggccatg
     3961 gcctcgatgg tggtgcgcac cacgttgttg tacgggtcct gctcggtcag gctccagccc
     4021 gaggtctggc agtgcgtgcg cagcatgagc gacttgggtg acttggcgtc gaagcccttc
     4081 atgatgcggc accacaagag ccgcgccgcg cgcatcttgg cgacctctaa atagaagttc
     4141 atgccaatcg cccagaagaa agagaggcgc ccggcaaact cgtccacgtc cagcccggtt
     4201 gcaatggcgg tcttcacata ctccttgccg tcggccagcg tgaaggccaa ttcgagtgcc
     4261 tggttggcgc cagcctcttg catgtggtaa cccgaaatcg agatcgagtt gaacttgggc
     4321 atgttcttgg ccgtgtagcc aatgatgtcg ccgatgatgc gcatgctggg cgcgggcggg
     4381 tagatatacg tgttgcgcac catgaactct ttcagaatgt cgttctgaat cgttccagac
     4441 agtttgtcct gcgatacgcc ctgctcttcg gccgccacca cgtaaccggc cagcaccggc
     4501 aacaccgctc cgttcatcgt catggagacc gagaccttgt ccagcggaat ctggtcgaac
     4561 aggatcttca tgtcctcgac cgaatcaatc gccacaccgg ctttgcccac atcaccggtc
     4621 acacgcgggt gatcggagtc gtagccacgg tgcgtagcga ggtcgaaggc gaccgacacg
     4681 ccctgcccgc ctgctgccaa tgccttgcgg taaaacgcgt tcgactcttc ggcggtggaa
     4741 aaaccggcgt actggcggat cgtccagggg cgcacggcgt acatggtggc ttgcgggcca
     4801 cgcaggtagg gctcaaaacc cggcaaggtg tcggcatact gcaggctggc cgtatccgct
     4861 gcggtgtaca ggggtttgac cgcgatgcca tcgggcgtga tccagttcag cgcatctaca
     4921 ttgccaccag gagcggactt ggcagcggcc ttggcccagg cgtcgagcgt ggcggatttg
     4981 aattcaggtg tcttgtcggt catggcgaag tccgttgcgc ggtggatggg ttgcccaaaa
     5041 ttggcgccac ccacggtccg cagatggtca cttttggctg gcagcgagtt tacattattt
     5101 ataattattc attcaaaata tggattgaag cttacaattc ccgcccatgt ccgctctgat
     5161 gtcagaaatt cgcccccctg cctcactttc tccacgtgcc ctgtacgaag aagtggcgga
     5221 gttgctgcgc cagcgcattt ttcggcgcga gctggagccc ggcagctgga ttgacgagct
     5281 gaaaattgcc gaggactacg gtatcagccg cacccccatg cgcgaagcgc tcaaggtgct
     5341 ggcagccgaa ggcctggtca ccatgaaggt gcggcgcgga gcctacgtga ccgaggtcaa
     5401 cgaaaaagac ctcaccgacg tgtaccacct gctctcccta ttggagagcg acgccgcggg
     5461 cgtggttgcc gtccgggcca cggacgcaca gttgcgtgaa ctccagacgc tgcacgacga
     5521 actggaaaca gcagcctgcc ccggcaagca ggaccgggaa cgcttcttcg agatcaatga
     5581 gcactttcac atgcggttgc tggagattgc cgacaaccgc tggcgtgacc agatggtggc
     5641 cgacctgcgc aaggtcatga agctcaaccg ccaccactcg ctgttcaagt ccgggcgcat
     5701 cgaagagtcc ctgcaagagc accgcgccat catggccgca ctgctggcgc gtgacccggt
     5761 ggcgaccatg gaaaccatgc gcgcccattt cagcaacggt ctggaagcgg ccgtttgacc
     5821 tgagagccag cttgtctaga atgggtgtcc aatacacaca cgttggacgc ccattcgcgc
     5881 tctgcagtac atttcgccct tccggcggat tggtagccgc gctcatacat gacagataac
     5941 aactctccga ctccgtccgc cgatgccgtg aagcagacat tgctggaata ccaggccatt
     6001 ctggaaaacg cgtctgtcgg cattctgttt tcgcgcggcc gcaaggtgct gcattgcaac
     6061 cccaggttct ccgaaatatt tggctgggaa caaggtgaat tggtaggcca acccgccacc
     6121 gtcctctatt tttcgcccga agactactcg gagttgggcc ggcttgcggg tccggtgctt
     6181 gccagaggcg agttgctgga catcgagcgc gtcatgcgcc gcaaggacgg cagtggtgtc
     6241 cattgccacc tcaactccag ggcgatcaat ccccagaaca cggcagaggg aaccatctgg
     6301 atcgtcgaag acatcggtga gcgcaaacgt gccgaaaccg aaatgaagga cctgatgctc
     6361 aagcagcagg ccattctgga aaacgcatcg ctaggcatca tgttcaccag caacgggcac
     6421 atcgtccatt gcaatccgcg ggtggaagcc atcttgaact ggccccaggg aacactggag
     6481 gggcaaaaag ccggtgtttt tttctccagc atggaggact atgcactctt tggcaagcag
     6541 gttggcccac aactcgcaag gggagaactc gttgacgtca gttggctgaa ccagtgcaag
     6601 gacgggagca ccatctggtg ccgccacctg gccaaggcgc tgaccaccac agacggttcc
     6661 aagagcacca tctggattac tgaagacatt tcggaaaaac gggctgcgca ggaagcgctg
     6721 caaaaagccc accaggaact cgaacagcgg gttgaacagc gcacggaaga actggcggct
     6781 gccaatacgc gcctgttgtc cgaaattgcc gaacgcaaac aaatcgagaa caaccttcgc
     6841 gcaagcgaag cgcgatttcg tgatctatcg gaattgtcct ccgactggtt ctgggagcag
     6901 gatgcagatt tccggtttgt cgagcttatg acaggcccca ggtatacgca gcttccacca
     6961 ggcgatacct atggaaaaac ccggtgggag ttgcccattc ttggcgtaac accagcgcaa
     7021 tgggagcaac atcgcgcgct acttgcagcg cacctgccat accgggaatt tatctaccag
     7081 ctggagctac ccgacggcga acgccattgg ctctctgtca gtggcaaacc catattcgac
     7141 aatggtgtct ttatgggcta cagaggtaca ggaacggaca tcacacagcg caaggcgacc
     7201 gagcagcaga ttgaatacct ggcctaccac gacccactca ctggcctacc caaccgcatg
     7261 ttgctggagg accgcatgca gcaaagcctg gcgcatgcag aacgcaacca tttccggctg
     7321 gcactgctgt ttctcgatct cgacaacttc aagaaaatca acgactcact gggccatgcc
     7381 actggcgaca ttcttctgaa ggaaatcgcg tctcgcctgg gtgaatgtgt gcgtgacacc
     7441 gacaccatca gtcgccaggg cggcgatgag tttgtcctga ttctcaatga cctgccagac
     7501 cctgacgcgt gcctgcccgt tttggtcaag atactcaaca ggctgcaaga ccccttcatg
     7561 gcagatggca acgaactctc gacatctgtt tccatcggta ttgccgtgta tccggaggac
     7621 ggaagggatt tcgagacgct acgcaagaag gcggacatgg ccatgtaccg cgctaaggaa
     7681 gcaggacgca atacctacca tttcttcgac gacgccatga acgacgaagc ggtggaacat
     7741 ctccagatgc gcaacggtct gcgacgggcc ctggagcgca gtgaactggt gctgtattac
     7801 cagccgcaaa ttgatctcgc cagtggcagg gtcgtgggga cagaagcgct tttgcgctgg
     7861 aatcacccgg actttggcct ggtcgcgcca ggccggttca tccctatcgc cgaggacagc
     7921 gggttgatcg ttcccattgg ggaatgggtc atcacagagg catgccgaca agctgtggca
     7981 tggcaaaaag cggggctgcc cgcactgcaa atggcggtca acctgtccgc cgttcagttc
     8041 aaacgcggga acgtggagca gattgtgatc accgcactgc agcaatccgg actggccccg
     8101 tcgcttctgg agttggagct gaccgaatcc atcctgattg agaacgtaga gcaggtactg
     8161 tccaccgtca agcggctcaa gcaactggga gtcaaactat ccatcgacga tttcggtacg
     8221 ggctactcta gtctctccta cctgaagcgg tttgacatcg acaagttgaa gattgaccag
     8281 tcctttatcc gcgatctggc taccgatacg gacgacgcag ccattgtgaa tgctgtgatc
     8341 cagatggcgc gcagcctgaa cctgaaaacg attgccgagg gtgtggaaca ccccgacatg
     8401 ctggatctgt tgcgtggatt tcagtgcgac gaggtccaag gctattactt cgcccggcct
     8461 atgcccgcag acgagttttc ggcctatctg tcaggcaact agcaggcacc cgcttgggtc
     8521 catacttcgc gcgctggcag ccccttgaga cgatggtcac tccacacctg acgccaacgg
     8581 cgtgcacccg gcagcccgtg gcgcaagccc agcatatggc gagcgatggc ataccacggg
     8641 gttccatgcg cctgcgcttc tcgttccatg tactgcacca tctgctcttc aactgattca
     8701 cgcgacatat ccaccaccgc agaaccatag aactcagcat cccagcggct gagccaccac
     8761 gggttgtgat aggcctcccg gcccaccatc acaccatcca catgcggtaa ttcctgatgc
     8821 acctgttctg aagtgatgaa cccgccatta atcgctattg tgagatgagg gaaatcctgt
     8881 ttcagctggt gcaccacggc gtagcgcaac ggtggtattt cgcggttttc cttgggtgac
     8941 agccctttga gccaggcatt gcgggcatgg acgataaaca ccttgcaacc cgcatccgcc
     9001 accgtcccca caaaatcgcg aacaaagcca tagctttcgt cccggtcaat accaatgcgg
     9061 tgcttgaccg tcactggcac atccaccaca tccaccatgg ccttgacgca ctcggccacc
     9121 aggggcgctt cgtgcatcaa acaggcaccg aaggcacccc gctgaacacg ttcgctgggg
     9181 caaccgcagt tgatgttgat ctcgtcataa ccccagtcct gccccagtct ggcacagtgc
     9241 gccagatcag cgggctcgct gccaccgagt tgcagggcca gcggatgctc ttcgacattg
     9301 aaacgcagat ggcgcggcac atccccgtgc agcagggcac cggtagtcac catctcggta
     9361 tagaggagcg catgccggct cagcagacgg tgaaagtaac gacaatgtct gtcagtccag
     9421 tccatcatcg gggccacgga catgcgccat gggttgagtt cagccgccac gttgttccac
     9481 ttcaggtcgc gcaccaggga gcggtattgt gcaccagccc catccacagc gcccaggccg
     9541 aactggccgc cagtgccgcg cccgcgatac gtacacccca ggcaccacta tcaggactat
     9601 gtccgcgcaa cttgagccat aaccaggggg cggcggtcat nnnnnnnnnn cactggtgcc
     9661 ctgtgcaaac aatgccatga ccaaggcgcg atccaatggg ccacccgcca tcgccgcaac
     9721 cagcaacgcc gaatacagca agccacacgg cagaagagcc cacaaaacac caagcaccag
     9781 aggagcaccg ccggattgcc cgccccaacc tcgcgctttg gcccacactt tgcgtccaac
     9841 ggactccagc cacaccggct gccgggccct gaccatcagc accagcccga gcaatgccgc
     9901 agccacgtga aacaatgtcc agacaggacg caaggctgcg gactgcaccg taagccaacc
     9961 caatccctgc atggaggcag cggccagccc gcccagcaag gagtagccag cgatacgccc
    10021 cacctggaac gtcaatatcg cggaattttt gttggggcct gccgcctgcc cgattccggc
    10081 acacgcggca ccacacatgg caacacagtg cggtccgccg accaggccca taagcagcgc
    10141 tgtcagcgca agtgacgatt gcatccgtgg atgctaaatg atcttggaga acttggcccg
    10201 agtgcggtcg gtctgcaaat agcgatcaaa aaccatagcc acggcacgga cgaagaacca
    10261 gccctgcgcc gtcacctgga taccgtgggc gtccaccacc accatgccct gctcctgcag
    10321 cacctgcaac gcttccaatt ccttggcgaa gtaagccgaa aaatcaatca ggtacgatag
    10381 attaatagac tcaaacaata actgcccctg gcacatcaac gccatgatga ctgcacggcg
    10441 caccaggtca tcgcgtgaaa gcgccaagcc ccgcacgacg ggaaagcggc cattgtccaa
    10501 ggcgtcgtaa tattcttcca gggtcttggc gttttgactg taagtcgcac cgatacgacc
    10561 aatgctggat acacccaggg caatcagatc gcagtctggc tgggtactgt aaccctggaa
    10621 gttccggtga agcctgcctt ggcgcttggc aacagccaga gcatcgtcgg gaagcgcaaa
    10681 gtggtccata cccacataga cataaccagc gctcatgaaa gccgtgagag aacgcgacaa
    10741 catggagatc ttggatcccc ctgttggcaa ctccacgatg gcaatacggc gctgcggctt
    10801 gaatcgctca ggcaaatggg cgtaggcata caaggcaatt cggtctggcc tgagctgcgc
    10861 cacctgcgcc agtgtccggt caaaagattc agggctttgc ttgggtagcc cgtagatcag
    10921 gtccacattg atggactcga aaccacgggc gcgcgctgcc gccaccagcg caaacacttg
    10981 ttcagcgggc tgtacgcggt gtaccgcctt ctgcactgca ggatcaaagt cttgaacccc
    11041 aaaactcagc cggttaaaac caagttctgc cagcgtatcc agccgccctg cgtccacggt
    11101 acgcggatcc acttcaatcg agtactcgcc gccaggggca aacgagaaat tccggcggag
    11161 catggccatc agctcacgca actcgtcatc cgacaaaaaa gtgggagttc caccgcccaa
    11221 gtgcaactgg ctgacgacct gccctgtacc catttgctgg gtatgcaggt ccacctcacg
    11281 actcaggtag cgcagatact ccgcagcctt gtcatggtgt ttggtaatga tcttgttgca
    11341 agcacagtag tagcacagcg attcacaaaa cggaatatgc acataaagcg acaacggcaa
    11401 cgccaacgca gcagccccac tacgcctttg cgtgagcgcc tgggcgtaat cttctgcagt
    11461 aaatgcctca acaaaccggt cagccgtcgg gtaggacgtg taccgcggac cggacacgtc
    11521 ataacggcgc aataaatcat cggtaacggt ttcgacttca gcgctcatga atgctccatc
    11581 tttagctggg ctaatatgcc cgaacccatc gaatttccat ttgatctgca tcaagtacca
    11641 agaaggtggg tacactcgca attgacgtga atcagaggat gcatatgttc gaccaggcca
    11701 ccaagcccaa tcacaccagc catacgcaca cgcccatcgc tatcaagccc gcggctgatg
    11761 ctgtggacat gcatccgcag gcaatcaagg tggcctgttc caattgcaat cttcgcgagt
    11821 tgtgcatgcc gttggggctg agtccggttg aactcgaccg gatcgacgat gtggtggcca
    11881 gccgtcgcaa aatcaagcgc ggcgccacgt tgtttcgtaa cggcgaaaaa ttccacaatt
    11941 tatatgccat tcgcacaggt ttcttcaaaa cttgtgtggc ttcggaagac ggccgtgatc
    12001 aggtcacggg cttccagatg gccggggaaa tcgtgggtct cgacggcatt gtcaatgagc
    12061 accacacctg tgatgccgtt gcactggaag acgcagaagt ctgcgtgatg ccttttgacc
    12121 gcattgaaga gctttcgcgt gaggtcaacg cactgcaacg ccatgtgcac aaaatcatga
    12181 gccgggaaat cgtccgggag aacggcgtca tgctgctact tggcagcatg cgggccgagg
    12241 aacgcctggc agcattcctc ctgaatctgg tgcagcgtct acacgcccgt ggattttccc
    12301 aatcggaact ggtgctacgc atgacccggg aagaaattgg cagctacctg ggcctgaaac
    12361 tggaaaccgt cagtcgcact ttctccaagt tcgtcgaaga cggcattgtc gaagtcaagc
    12421 aacggcatgt gcgtatcctg aatacggaag cactcaagga aatcgtcaat cccaaagtct
    12481 gcgactgaaa cagcaccgtt tctgccccgg ggcgcgcgca cgccctccta agttggttgg
    12541 tatttcaagg ggtctttgga gacggcttga cccccgtttg ggcgtggtta cgcgcctgca
    12601 ctgctggagc aagactctcc ggagtgtctg ccaatgtacg attgatttcg ataccttttc
    12661 ggtaatagtc ctcgtcgacc acatggtccg ttccattggc agcaaggtaa aaagtgatga
    12721 agctggcaac aaccacaatg gcgggaccgg aaatcaccag ccacacatgc ccaaaacgcc
    12781 accagggacg gggagaggtt ggttcagatt gagccatggt taatccttaa cggggaacaa
    12841 taaacaccga tttttcagaa accgtctgag ccgactgctg cgcggtg
//
LOCUS       DAOSJM010000017        12479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5085_length_12479_cov_4.670316, whole genome shotgun sequence.
ACCESSION   DAOSJM010000017 DAOSJM010000000
VERSION     DAOSJM010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12479
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5085_length_12479_cov_4.670316"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="PKX82_01280"
     CDS             <1..1816
                     /locus_tag="PKX82_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOF50906.1"
                     /translation="KSGAKDESKKLEWRGTPEAPESVGARLSHALVHGITDFITQDTE
                     EAYQDVLAKGGRPLHVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKSAVAHLLP
                     YIEAEKAAMVEAGGEAKAKGKIVIATVKGDVHDIGKNIVTVVLQCNNFDVVNMGVMVP
                     CHEILAKAKEEGADIIGLSGLITPSLEEMQYVAGEMQKDEYFRSRKMPLLIGGATCSR
                     VHTAVKIAPNYEGPVVYVPDASRSVGVAQGLLSEQAAQYIAELNADYDKVRQQHANKK
                     QTPLWPLAKARANKTPINWAAHQPTVPKFIGRRVFKNFDLGELAKYIDWGPFFQTWDL
                     AGPFPAILKDEVVGTEAVRVYSDGQRMLKRLIEGRWLTANAVVALYPANTVNDDDIEF
                     YTDESRTEVAMTWYGLRQQTEKQEIDGVMRPSRCLADFVAPKGVKADYAGLFAVTAGI
                     GVDKKEKYFLDDLDDYSAIMLKSIADRLAEAFAEALHHRVRTDLWGYAADEALSNEDL
                     IAEKYRGIRPAPGYPACPDHSVKKDMFALLQCDEIGMAVTESLAMTPAASVSGFYIGH
                     PDATYFNVGKIGEDQVQDLAQRRNLNQTELQRLLAPNL"
     gene            1866..2402
                     /locus_tag="PKX82_01285"
     CDS             1866..2402
                     /locus_tag="PKX82_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50907.1"
                     /translation="MNRTLSLSATASSPRFRLGWIAAGVFAISSTFATAAELQPLAFK
                     ATTQQLAMQTAGPVCAICGTVESLTEVQRESAPSGLGALAGAALGGWIANQVGGNDGK
                     TLATIVGLLGGMWAGNAAEKQIKKETVYVVAVRMEDGALRTLEQSSPIAVGTRVTVEG
                     SVIVPMTTPAVTGAGTTV"
     gene            2450..4213
                     /locus_tag="PKX82_01290"
     CDS             2450..4213
                     /locus_tag="PKX82_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOF50908.1"
                     /translation="MNMATAAPGAPNGPRPAPGRPEKLRLGDVLVQQKLISQEQLQQT
                     LDLQRTTGKKVGRLLIETGIITEELLANGLARQLRIPFVNLKTFPFRADVVKLLPEST
                     ARRFKALALEDKGDILLVALADPLDLFAYDELTRILKRNIGIAAVPESQLAAAFDRLY
                     RRTEEISGLARALEKDLGDAVDFGELTASVGIEGAPVVRLLQSLFEDAMQVGASDVHI
                     EPQENSLQIRVRVDGVLQTQTQADKRIGSALAQRLKLMAGLDISEKRLPQDGRFSVRL
                     KEHTIDVRLSTLPTTYGESAVMRLLNQGAAMRRLDNIGMPEDMLKRFREVLARTSGMV
                     LVTGPTGSGKTTTLYAALAEINAAELKVITVEDPVEYRLAGITQVQVNDKIELTFAKV
                     LRSCLRQDPDVILVGEMRDAETAEIGLRAAITGHLVLSTLHTRDAMSTPFRLLDMGVP
                     PFMVATSLQAVIAQRLVRLNCTECAAPATPTAQEQAWLTNMLATGEPLHAKRGMGCSA
                     CNGTGYSGRQGVYELLEMDATLTQAASQSNPTAFMRSARERMKGHTMAYHALSLVREG
                     RTTIAEAMRIGFEIDAGGDQE"
     gene            4220..5455
                     /locus_tag="PKX82_01295"
     CDS             4220..5455
                     /locus_tag="PKX82_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOF50909.1"
                     /translation="MAVYTWKGRNNRGEAVSGQLEAMTEGGVADQLLSIGVAPVHIAL
                     AEVPKESTGEGWLARLNRKPVVDEDLMLFSRQMYTLNKAGVPILRAFAGLQASATKPA
                     MVELFKDIRSSLDQGRELSAALARHSELFGGFYISMIRVGEMTGRLTEVFLRLNEHME
                     FERDVRERIKQAMRYPLFVMIAMAIAIVILNVFVIPVFAKVFAGFNAELPLITRGLIG
                     FSNWMVAWWPLLLALLAGAGVVIRGYLRTPEGRYRWDARKLKLPIVGDIILKATLARF
                     ARSFALSSQSGVPLVQALTVVAQTVDNAFIGSRIEQMRDGIERGESISRCAAATGVFT
                     PVVLQMINVGEETGELDNLLFEIAGMYERDTDYAIKGLSAAIEPILLAVIAVLVLLLA
                     LGVFLPLWNMGQAAMGKGG"
     gene            5527..5934
                     /locus_tag="PKX82_01300"
     CDS             5527..5934
                     /locus_tag="PKX82_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50910.1"
                     /translation="MFAHQFRAVQAQGELAAIKTTLGALRTALVIDHLERAVASKGQG
                     VAIAQRNPFLLLARVPANYAGAFEASKANAVPPGNWVFDSYCNCIGYGPMHPFALDAD
                     TEAPMVWFRISAPPGPLQITPVQAYRWQGELVN"
     gene            6081..6470
                     /locus_tag="PKX82_01305"
     CDS             6081..6470
                     /locus_tag="PKX82_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOF50911.1"
                     /translation="MRQVQRGFTLIELVMVIVILGVLAAVAIPKFVDLGSDAKAAALQ
                     GVIGGISSASAVNYAARSVTTGNGTAINAGTGCSAAAAAILQGGIPSGYTVSGTTSGT
                     TGTANSCTVTQTSTSNTGTATIISVTP"
     gene            complement(6514..8667)
                     /locus_tag="PKX82_01310"
     CDS             complement(6514..8667)
                     /locus_tag="PKX82_01310"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOF50912.1"
                     /translation="MALTDTTNPAADRTVTVLAGMAFLLPAVGSASEMLAQDTLKSAI
                     AACSVFLAALVFLWNKRPATAPWLWHSMLGLPLTLMVYALGSVVWSHGYLASVETTRW
                     FLLALLAWLGLNAITTNNLPRLLWGIHAGLVVASLWAMLQFWLDFSLFAQGPPPASTF
                     YNRNFFGEYAVCALPFSVWLLSTVKSPRGAIPLAMSLAAVVTAILMTGTRSALIALAL
                     TAPLLVATVVRYRTQLPCGKWTRRTTISVVATFLTALTVLGNIPSYAVGITQERTGVT
                     AWNRGTVRVASMAQPSEYTEGSFSVRSQMWRATLRMAMENPFAGVGAGAWEVHIPRFQ
                     RDDTVLEIDYYAHNEYLQLLSEYGLPLGGFVLAFLAAFWIHSVDTTWRVEKTAPQEAP
                     LRGFVLTGMLAMGIVSMAGFPWHLAGCGVLFALSFSLLCGSDIRMGSHLGGAFPPPTR
                     KTYVHRAVLVTLLICMAIAVYTTIRAVHTERTLVQALLLAKNLSKSKDTWGPEQQASK
                     DRMLNLVREGIHLNPHYRKLTPLAAEFLMVQGDWANAAWILKSVTDSRPHVAALWKGQ
                     ALAHAQMHQSSPALNALQQVQRLRPDTKATQNLEIVVLSNVGQAQTAAQLLRTYFATR
                     NYDYEMVQAGYALGLKLRDNALAVQALQLRNQTWPEQAADGFFRMGLVHAQTMPPDHA
                     AALEAFASGLSAVPAQEKENYLRQVPLPYRNRLDK"
     gene            8852..9322
                     /locus_tag="PKX82_01315"
     CDS             8852..9322
                     /locus_tag="PKX82_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOF50913.1"
                     /translation="MHGGFTLIELIMVIVLLGVLAVFAGPRMLRSSDFYARGFHDETL
                     AYLRYAQKTAIAQRRTVCVTFGASSVTLAIASTAAVSNCSTAGTLLGPKGENPAVLNA
                     RSGVSYVSTPTSFNFDGLGQPLTTAGAQQPTQTFQVSGASRAITVETATGYVHE"
     gene            9315..9746
                     /locus_tag="PKX82_01320"
     CDS             9315..9746
                     /locus_tag="PKX82_01320"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOF50914.1"
                     /translation="MNKLRHRGFTLIELIIFIVVVGVGLAGILTVSNNVVKSSADPMV
                     RKQSMALADSILEEILQKEYADPDGVQGNETTRDTMDDVDDYNGKTKAIFTDWPSALS
                     TYTVSISVTPSTLGSVVAKKVTVTVSGGVHVISLSGYRTNY"
     gene            9755..10576
                     /locus_tag="PKX82_01325"
     CDS             9755..10576
                     /locus_tag="PKX82_01325"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOF50915.1"
                     /translation="MRRTHRGFTLIELVMVIVILGVIGGMVSVFMKRPIDAYFDTARR
                     AALADVADTTLRRMARDIHKALPNSIQVPNNQCVEFIPTKTGGRYRVEDKSGAPGTGL
                     DFSAADTGFNMLGSNSARPADQQIADGDLIAVYNLGIAGSNAYNGDNTAAVSGTPSES
                     GSPAETTITIASKQFPLPSGSNRFHVIPGTEKVVSYVCSGGTLYRNANYTYSSSCPAP
                     TPGTTPVIATNLGTCNFVYNGSDLQRNGLVQMTIAFTNSGETVTQYHEIHVNNTP"
     gene            10573..10962
                     /locus_tag="PKX82_01330"
     CDS             10573..10962
                     /locus_tag="PKX82_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50916.1"
                     /translation="MIIGQRLQQGFAAIAAIFLVVILAALGAFMVSFSNTQHLTAAQD
                     IQGSRAYWAARAGLEWGIASVTTVCPTSPTSLNVEGFTVEVKCTPTTYTDSGTVNLFE
                     ITALAKSTGAVGSPGYIERSLTASMER"
     gene            11048..>12479
                     /locus_tag="PKX82_01335"
     CDS             11048..>12479
                     /locus_tag="PKX82_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOF50917.1"
                     /translation="MVAASSAHAATYGYRSDTFAYDTPSASASSVAWHSTGASPACTT
                     YPNGDDDWADIVFPTGFTFTFGGVSYSSVRVYSNGILAFPTDVSGFHRDYTSQALPIT
                     TGPVAYTGCPSAVPANLMMVYWLDIVAGIANSTAGASIQYELLGSAPNRRLVISWVNV
                     KLYNTTTRYNFQVALQESLPGVNGNFKYQYTTGSSTGANAAVGVQLTSTDYTQYAYNQ
                     NFIDTTLGTAILWYPANQLAGKSAEYRFDESSWTGTVGEVKDTSGNLQNASRTTANAA
                     NVAGGKLCRGATFTNNTSTTTIDAVATPLVPANRGSVSFWYKSSAAWNSANAMLFDAT
                     TVAARPFFLMKRSTGALRFAVTDSAGTVRTAETSTAYTYAAGTWHHIAVSWAFTPGTN
                     QTVQQIMLDGVLVNTTGTTPYRTTSSGAIATPSTLYIGDNRTSGITPSTGSPNGANGT
                     IDEVYVYALDINATQAAADMALTRPTC"
BASE COUNT         2599 a         3596 c         3774 g         2510 t
ORIGIN      
        1 caagagcggt gccaaagatg aaagcaaaaa gctcgaatgg cgcggcacgc ccgaggcacc
       61 ggaatcggtg ggggcacgcc tctcccatgc gctggtgcac ggcattacag acttcattac
      121 ccaggacacg gaagaagcct accaggatgt actggcgaag ggtggccgtc cgctgcatgt
      181 gatcgaaggt ccgctcatgg atggcatgaa cgtggtgggt gacctgtttg gccagggcaa
      241 gatgtttctg ccccaggtgg tgaaaagcgc gcgcgtgatg aagagcgcag tggcgcacct
      301 gctgccctac atcgaggccg aaaaggctgc catggtcgag gccggcggtg aggccaaagc
      361 caagggcaag attgtgattg ccaccgtcaa gggcgatgtg catgacatcg gcaaaaacat
      421 cgtcaccgtg gttttgcagt gcaacaactt cgacgtggtc aacatgggtg tgatggtgcc
      481 ctgccacgaa atcctggcca aggccaagga agagggcgca gacatcattg gcctctccgg
      541 actgattact cccagcctgg aagagatgca gtacgtggcg ggcgagatgc agaaggatga
      601 gtacttccgt agccgcaaga tgcctctgct catcggtggc gccacctgca gccgggtgca
      661 taccgcggtg aagattgcgc ccaactacga aggcccggtg gtctacgtgc ccgatgcttc
      721 ccgcagcgtg ggtgtggcac agggcttgtt gagcgagcag gcggcccagt acattgccga
      781 gctcaacgcc gactacgaca aggtgcgcca gcagcacgca aacaagaagc aaactccctt
      841 gtggccactg gccaaagcac gggccaacaa aacgcccatc aactgggcag cgcaccagcc
      901 cacggtgccc aaattcattg gacgccgcgt cttcaagaac tttgatttgg gcgagttggc
      961 caagtacatt gactggggcc ccttcttcca gacctgggat ctggcgggtc cttttcccgc
     1021 catcctcaaa gatgaagtgg ttggcacgga ggccgtgcgc gtctactcag acggccagcg
     1081 catgctcaag cgcttgatcg aagggcgctg gctcactgcc aatgcggtgg tggcgctgta
     1141 cccggccaac acggtgaacg acgatgacat cgagttctat accgacgagt cccgcaccga
     1201 ggtcgccatg acctggtacg gcctgcgcca gcagaccgag aaacaggaaa ttgatggcgt
     1261 catgcgcccc agccgctgcc tggccgactt cgtggcgccc aaaggtgtga aggcggacta
     1321 cgcgggtctg ttcgcggtga ctgccggcat tggcgtggac aagaaggaaa aatacttcct
     1381 cgatgacctg gatgactact cggccatcat gctcaagtcc attgccgacc gcctggccga
     1441 agcctttgcc gaagccctgc accaccgagt gcgcactgat ctgtggggtt acgccgccga
     1501 tgaggcgctg agcaatgaag acctgattgc cgaaaagtac cgtggcattc gcccggcccc
     1561 cggttaccca gcctgcccgg accacagcgt caagaaagac atgtttgcac tgctgcaatg
     1621 tgacgagatt ggcatggcgg tgaccgagag cctggccatg accccggcgg ccagcgtgag
     1681 cggcttctat attggccatc ccgatgcgac ctatttcaac gtgggcaaga ttggcgagga
     1741 ccaggtgcaa gatctggcgc aacgccgcaa cttgaaccag acggaattgc agcggttgct
     1801 ggcgcccaac ctgtagtccg gatgtcgcgg ttgcgcgtta tggtgtctgc aacccaagga
     1861 gcaccatgaa ccgtaccctt tcactttctg cgactgcctc gtctccccga tttcggctgg
     1921 gatggatcgc ggcgggtgtt tttgccatca gcagcacctt tgctacggca gccgagctcc
     1981 agccccttgc tttcaaagcc acaacgcagc agttggccat gcagacggcg ggtcctgtgt
     2041 gcgccatctg tggcacggtt gaatctttga cggaggtcca gcgcgagtct gcgcccagcg
     2101 ggctgggcgc cctagcaggt gccgcactgg gtggctggat tgccaaccag gtcggtggca
     2161 atgatggaaa gaccttggcc accatcgtgg gcttgctggg cggcatgtgg gccggtaatg
     2221 cggccgagaa gcagatcaag aaggaaacgg tgtatgtcgt ggccgtgcga atggaggatg
     2281 gcgcactcag gacactggag cagagcagcc cgatagcggt gggcacgcgc gtcacggtcg
     2341 aaggatccgt gatcgtcccc atgaccacac ccgccgtaac cggagccggc accacggtct
     2401 gacagtgcca cgtgctatca tttttgcgaa cccgcaaaag gaatagcgca tgaatatggc
     2461 aactgcagcg cccggcgcgc ccaatggccc gcgcccggcc ccgggccgtc cggagaagtt
     2521 gcgtcttggt gacgtactgg ttcagcagaa gcttatctcg caggaacaac tccagcagac
     2581 gctggatttg cagcgcacta ccggcaaaaa ggtcggtcgg ctgctcattg aaaccggcat
     2641 catcaccgaa gagttgctgg ccaacggctt ggcgcgccaa ctgcgcattc ccttcgtcaa
     2701 cctcaagacc tttcccttcc gcgccgatgt ggtgaagctg ttgcctgagt ccaccgcccg
     2761 gcgcttcaag gcgctggcgc tggaagacaa gggcgatatc ctgctggttg cgctggccga
     2821 tccgctggat ctgtttgcgt atgacgaact gacgcgcatt ctcaagcgca acattggtat
     2881 cgcagcggtg cccgagagcc agttggcggc ggcgtttgat cgcctgtacc gacgcacaga
     2941 agaaatcagc ggcctggccc gagccctgga aaaggacttg ggcgatgcgg tggactttgg
     3001 cgagctcacg gccagtgtcg gcattgaggg tgcgccagta gtacgcctgt tgcagtccct
     3061 gtttgaggat gcgatgcagg tcggtgcttc tgacgtgcac attgaacccc aggaaaacag
     3121 tctgcagatt cgtgtgcgtg tggacggtgt tttgcaaacc cagacgcagg cggacaagcg
     3181 tatcggcagc gcattggccc aacggttgaa gctcatggcg gggctcgata tttcggaaaa
     3241 acgcctgccc caggacggcc gcttctcggt gcgcctcaaa gagcacacca tcgatgtgcg
     3301 gttgtccacg ctacccacga cctatggcga gtcggcggtg atgcgtctgc tcaaccaggg
     3361 tgctgccatg cgtcggctgg ataacattgg catgccggaa gacatgctca agcgtttccg
     3421 cgaggtgctg gcgcgtacct ccggcatggt gctggtcacg gggcccaccg gttcgggcaa
     3481 gaccaccacg ctgtacgctg cgctggcgga aatcaatgcg gcagagttga aggtcatcac
     3541 ggtcgaggat ccggtggagt accggcttgc gggcatcacg caggtgcagg tgaacgacaa
     3601 aattgaactg acctttgcca aagtgctgcg gtcctgttta cgccaggacc cggacgtcat
     3661 tctggtgggt gaaatgcgcg atgccgaaac ggcagagatt gggttgcgtg ctgccattac
     3721 cggacatctg gtgctatcca ccttgcacac acgcgatgcg atgagcacgc cgttccgctt
     3781 gctggacatg ggcgtgccgc cgttcatggt ggccacctcc ctgcaggcgg tgattgcgca
     3841 gcgcctggtg cgcctgaact gcaccgagtg cgcggcgcca gcgacgccta ccgcgcagga
     3901 gcaagcctgg ctgaccaata tgcttgcaac cggtgagccg ctgcatgcca agcgcggcat
     3961 gggctgttcg gcctgcaacg gtaccgggta ctcagggcgt cagggtgtct acgaattgct
     4021 ggaaatggac gccacgctga cgcaagcggc atcgcaatcc aatccgacgg ccttcatgcg
     4081 ctctgcgcgt gagcgcatga aaggccacac catggcctac cacgccctgt cgctggtgcg
     4141 cgaagggcgc accaccatcg cagaagccat gcgcattggt tttgaaatcg acgccggcgg
     4201 cgatcaggaa taacccgcca tggctgtcta cacctggaaa ggccgcaaca accgcggcga
     4261 agccgtcagt ggccaactgg aggccatgac cgaaggtggt gtggctgacc aattgctctc
     4321 cattggtgtg gcgcctgtgc acatcgcgct ggcagaagtg cccaaggagt ccaccgggga
     4381 gggatggctg gcgcgcctga accgtaagcc cgtggtggac gaagacctga tgcttttctc
     4441 ccgccagatg tacacactga acaaggccgg ggtgcccatc ctgcgcgcgt ttgccgggct
     4501 gcaggcttcc gcgaccaagc ccgcgatggt ggagttgttc aaagacatcc ggtccagcct
     4561 ggaccagggg cgcgagcttt ccgccgcgct ggcgcgccat tcggagctgt ttggcgggtt
     4621 ttacatttcc atgatccgtg tgggggagat gacaggccgt ttgaccgaag tgtttctgcg
     4681 tttgaacgag cacatggagt ttgagcggga tgtgcgtgaa cgcatcaagc aggccatgcg
     4741 ttaccccctg tttgtcatga tcgccatggc gattgccatc gtcattctca atgttttcgt
     4801 cattccggtt tttgccaaag tgttcgctgg cttcaacgcc gagttgccac tgatcacgcg
     4861 ggggttgata ggtttctcga actggatggt tgcctggtgg ccccttctgc tggcgctcct
     4921 ggccggtgca ggggtggtga ttcggggcta tttgcgcaca cccgagggtc gctaccgctg
     4981 ggatgcgcgc aagctcaaac tgcccatcgt gggcgacatc attctcaagg ccacactggc
     5041 ccggttcgcc cgcagctttg cgctgtccag ccagagcggt gtgccgctag tgcaggcgct
     5101 gaccgtggtg gcgcaaacag tggacaacgc ctttattgga agccgcattg agcaaatgcg
     5161 cgatggcatt gagcgtggcg agagcatctc gcgctgcgcc gctgcgacgg gcgttttcac
     5221 gccagtggtg ctgcaaatga tcaatgtggg ggaagagacc ggcgaactcg acaacctgct
     5281 gttcgagatt gcaggcatgt acgagcgcga caccgactac gcgatcaagg gtttgtccgc
     5341 agcgattgag ccgatcttgc tggcagtgat tgccgtgttg gtcttgttgc tggcgctggg
     5401 ggtattcctg ccgctgtgga acatggggca agccgccatg ggcaaaggcg gctagccttg
     5461 cgctggcaaa gtacgcgtgc tgcggaatgg agtttgctgg tggtggtgct tttggtggtg
     5521 atggggatgt tcgcccacca attccgggcg gtacaggcgc aaggagagct ggccgccatc
     5581 aagaccacgc tgggtgccct gcgcaccgcc ctggtgattg accatttgga gcgtgctgtg
     5641 gcttccaaag gccagggtgt tgcaattgcg caacgcaacc ctttcctcct gttggcccgt
     5701 gtgccggcca actatgctgg cgctttcgag gcaagcaaag ccaatgcggt accccccggc
     5761 aactgggtgt ttgacagcta ttgcaattgc atcggctatg ggccgatgca cccttttgcg
     5821 ctggatgcag acaccgaggc gcccatggtc tggtttcgca tcagtgcgcc acccgggccg
     5881 cttcaaatta cccccgtcca ggcgtatcgc tggcagggtg agctggtcaa ttgagtcaaa
     5941 aagttgaatc atttcagcaa cttggcgcta aaagttgatg cataacagta catacatgct
     6001 ctagaatttg tagcgtaagc cgcgcgcggc gggtcgcggc gataaaatcc tttaaaaaca
     6061 cagcttggaa aggcgaaatc atgagacaag tgcaacgcgg ttttactctg attgaattgg
     6121 tgatggtgat cgtgatcctc ggcgtattgg ccgcagtcgc gatacccaag tttgtcgatc
     6181 tgggcagcga tgccaaggct gctgcgctgc aaggcgtgat tggtggcatc agctccgcca
     6241 gcgcggtgaa ttacgctgca cgcagcgtga caaccggtaa cggaactgcg atcaatgccg
     6301 gaactggctg ttccgctgcg gctgcagcaa ttttgcaggg cggcattccc agcggctaca
     6361 ccgtatcggg gacgacttct ggcactaccg gaactgcaaa cagttgcacc gttacgcaga
     6421 cttctacttc caatacgggt acagcgacca tcatcagcgt taccccctga tcgtggtttc
     6481 ccaatttcca aaggtcgccc tagggcgacc tttttacttg tcgagtcggt ttcggtaagg
     6541 caaaggcacc tggcgcaggt agttctcttt ttcctgtgca ggcacagccg ataaccctga
     6601 cgcaaacgcc tccaacgcag cagcatggtc tggcggcatg gtttgcgcgt gtaccagacc
     6661 catgcgaaag aatccgtccg cagcttgctc cggccacgtc tggttgcgca gttgcaaggc
     6721 ctgcaccgcg agtgcgttgt cgcgcagctt cagccccagt gcatagccgg cctgcaccat
     6781 ttcgtagtcg tagttgcgcg ttgcaaaata ggtgcgcagc aactgggctg ctgtttgtgc
     6841 ctgccccaca ttgctgagca ccacgatttc cagattctgg gtggccttgg tgtctggccg
     6901 gagtcgctgc acctgttgca gcgcgttcag agcgggcgaa ctttggtgca tttgcgcgtg
     6961 cgccaacgct tggcctttcc acagtgcagc cacgtgtgga cgggaatcgg tgaccgattt
     7021 caaaatccac gctgcattgg cccagtcacc ctgcaccatc aggaactcgg cggccaaggg
     7081 cgtgagcttg cggtagtggg ggttcagatg aatgccttcc cgcaccagat tcagcatgcg
     7141 gtctttggag gcttgctgtt cgggacccca agtgtctttt gacttggata gatttttggc
     7201 aagcagaagc gcctggacga gggtgcgctc cgtgtgtact gcgcgaatgg tggtgtacac
     7261 ggcgatggcc atgcaaatca gtagtgttac caggactgca cgatggacgt aggtcttacg
     7321 ggttggaggc ggaaatgctc ccccaaggtg gctgcccatg cggatatctg atccacaaag
     7381 caacgagaaa ctcaacgcaa acaacacgcc acaccccgcc agatgccagg gaaagcccgc
     7441 catactgacg atgcccatgg ccagcatgcc tgtcagaacg aacccacgca gtggagcttc
     7501 ctggggcgca gtcttttcta cgcgccaagt ggtgtccacg ctgtgtatcc agaatgcagc
     7561 caggaacgca agcacaaatc cacccagagg caaaccatat tcgctcagga gttgcaggta
     7621 ttcgttgtgt gcgtagtagt cgatttccag tacggtgtcg tcacgctgga acctcggaat
     7681 atgaacttcc catgcgcctg cacccacgcc agcaaaaggg ttttccatcg ccatgcgcaa
     7741 cgtggcacgc cacatctgcg accgaacgga gaaggagccc tctgtgtatt ctgaaggctg
     7801 ggccattgac gcaacgcgta ccgttccacg attccatgcg gttactccgg tgcgctcctg
     7861 cgtgataccg acggcatagg acggtatgtt tcccaacacg gtcaatgcag tcaagaaagt
     7921 cgcaacaacg gaaatcgtgg tgcgacgggt ccattttccg cacggtaact gcgtccggta
     7981 acgcacgacg gtggctacga gaagtggtgc ggtcagtgca agtgcgatca aggcagaccg
     8041 cgtcccggtc atcagaatgg ccgtgacaac ggctgcaagc gacattgcaa gtgggattgc
     8101 cccgcgaggc gatttgacag tgctcagcag ccataccgaa aagggcaggg cacataccgc
     8161 atattcacca aagaagttgc ggttgtaaaa agtggatgca ggcggcggtc cctgtgcaaa
     8221 caaggaaaaa tccaaccaga actgcagcat ggcccagagc gatgcgacaa ccagaccggc
     8281 gtggattccc cacagcaacc ggggcaggtt gttcgtggtt atcgcattca aacccaacca
     8341 cgcgagcagt gccaacagga accagcgggt cgtctcgacc gaggctaggt atccgtggct
     8401 ccagaccaca ctacccaagg cgtacaccat aagggtaagc ggcaacccca gcatgctgtg
     8461 ccaaagccat ggtgcagtgg ctggacgctt gttccagaga aaaaccaggg cggccagaaa
     8521 taccgaacag gctgctattg ctgacttcaa ggtgtcctgc gcaagcattt cactggccga
     8581 gcccaccgcg ggtagcagaa acgccattcc tgcgagaacg gtgaccgtgc ggtctgcagc
     8641 agggttagtg gtgtctgtaa gtgccatgta ttcgatcata ggggcaagca tgcgctgtct
     8701 ggttgtgtgg tgcatggagc tcaaatagaa tgaaagcccc cgtgtatatt gcctaaccag
     8761 ctacaaaata cgtagcaaaa tgcacctcat gccgcagtca ccgcaacgga caaccgctat
     8821 tggctttcaa aggtcgggag gaggcctttg tgtgcacggc ggatttacac tgatcgaact
     8881 gatcatggtg atcgtgctgc tgggtgtgct ggcggttttt gcaggccccc ggatgttgcg
     8941 ctccagcgac ttttacgccc gcgggtttca tgacgaaaca ctggcctatc tgcgctatgc
     9001 ccagaagacg gcgatcgccc agcggcgcac ggtgtgtgtc acctttgggg catcgtctgt
     9061 gacgctggcc atcgcgtcga cggcggccgt gtccaactgc agtaccgctg gcacattgct
     9121 gggacccaaa ggcgagaacc ccgccgtttt gaacgcgcgt tccggcgtaa gctatgtcag
     9181 tacgccgacg agttttaact tcgacggctt ggggcaaccc ctcaccacgg ctggggcaca
     9241 gcagcccaca cagacttttc aggtcagcgg cgcgtcccga gccatcaccg tggagacggc
     9301 cacgggctat gtccatgaat aagctccgtc accggggatt caccctgatc gaactcatca
     9361 tcttcattgt ggtggtggga gttgggctgg cggggatact gacagtgtcc aacaacgtgg
     9421 tgaagtccag cgcagacccc atggtgcgca agcagtcgat ggcgctggcg gactccatcc
     9481 tcgaagaaat cctgcagaag gagtatgcag atcccgacgg tgtgcagggc aatgaaacca
     9541 cgcgtgacac catggacgat gtggacgatt acaacggcaa aaccaaggcg atttttacag
     9601 actggcccag tgctttgagc acctataccg taagcatttc cgtcactccc tccacgctgg
     9661 gtagtgtcgt ggccaaaaaa gtcacggtga cggtgagcgg aggcgttcac gtgatttccc
     9721 tctcgggcta ccggaccaac tattgagtat ggacatgcgc cgcacacacc gtgggtttac
     9781 gctgatcgag ctggtgatgg tgatcgtcat cctgggcgtg attggtggca tggtttccgt
     9841 tttcatgaaa cggcccatcg acgcctattt cgataccgcc cggcgcgccg cgttggcgga
     9901 tgtggcggac accaccttgc ggcgtatggc gcgggatatt cacaaggcac tccccaacag
     9961 catccaggtg ccaaacaacc agtgtgtcga attcattccc accaagacag gtggccgcta
    10021 ccgggtggaa gacaaatcgg gtgctcctgg aacaggcctc gacttcagtg ctgcagatac
    10081 cggtttcaac atgctgggca gcaacagtgc tcgtcctgcg gaccagcaaa ttgcagacgg
    10141 tgacctgatt gccgtgtaca acctgggcat tgccggatcg aatgcctaca acggtgacaa
    10201 cacagctgct gtcagtggca cgccttcgga atccgggtct ccagcagaaa ccaccatcac
    10261 cattgccagc aaacagttcc cgctgccgtc cggcagcaac cgctttcatg tcatcccagg
    10321 cacggaaaaa gtggtgtcgt atgtctgcag cggtggaacg ctgtaccgaa acgccaacta
    10381 tacctatagc agttcctgtc cagcacccac gccgggaacg acgccagtga ttgcgactaa
    10441 tctcggcacc tgcaattttg tctacaacgg ttcggatctg cagcgcaacg gactggtgca
    10501 aatgacgatt gcgtttacca attcgggtga gacagtgacc cagtaccacg agattcacgt
    10561 caacaacacg ccatgatcat cggtcaacgc ttgcaacaag gattcgccgc cattgccgcc
    10621 atatttctgg tggtgattct ggctgcattg ggcgcgttca tggtgtcgtt ttccaataca
    10681 cagcatctga ccgcggcgca ggacatccag ggatcgcgcg catattgggc ggcccgtgcc
    10741 ggattggagt ggggcatcgc cagcgtgacc accgtttgtc cgacatcgcc cacgtccttg
    10801 aatgtggaag ggtttaccgt tgaagtgaaa tgcaccccaa ccacttatac ggattccgga
    10861 accgtcaatc tgttcgagat caccgcattg gcaaagtcga cgggtgccgt gggtagtccg
    10921 ggctatattg agcgtagttt gactgcctcg atggagcgat agcgcgatgt acaagtattg
    10981 gaaagggtcg ccccgttatc cccggctttt tgcgcgctgg atggcttggt gcttctatgt
    11041 ctggctggtg gtggctgcat ccagtgcgca cgctgccacg tacggttatc gctcggatac
    11101 gtttgcctat gacactccaa gtgcgagcgc gagcagtgtg gcatggcaca gcactggtgc
    11161 gtctcctgca tgcacgacct accccaacgg ggatgatgac tgggcggata ttgtgttccc
    11221 gacgggcttc acttttacct ttggtggcgt gagctactcc agcgtgcgcg tgtactccaa
    11281 cggcatcctg gcgtttccga cggacgtctc ggggttccac cgggactata cctcccaagc
    11341 attgccgatc acgacgggcc ccgtggctta tacaggttgt cccagtgccg ttcctgccaa
    11401 tttgatgatg gtctattggt tggatatcgt cgcggggatt gccaacagca ccgcgggcgc
    11461 ttctattcag tatgagctgt tgggctccgc accaaaccgg cgtcttgtga tttcttgggt
    11521 aaacgtcaag ctctacaaca ccaccacgcg gtacaacttt caggttgcgc tgcaggaaag
    11581 cctgccaggc gtcaacggca acttcaaata ccaatacacc acaggttcct ccactggggc
    11641 caatgcggcg gtaggcgtcc aactcacttc cacggattac acgcaatatg catacaacca
    11701 gaatttcatc gacacgactt tagggactgc cattctctgg taccccgcca accagttggc
    11761 gggaaagagt gcggaatatc gttttgacga aagcagttgg acgggaacgg tgggtgaggt
    11821 caaagatact tcgggaaatc tgcaaaacgc ctcccgaaca accgccaatg ccgccaatgt
    11881 ggcgggtgga aagctatgcc gtggcgctac ctttaccaat aacacgtcga ccaccacgat
    11941 cgacgcggtg gcaactcctc tggtgcccgc caatcgtggc tcggtgagtt tttggtacaa
    12001 atccagcgca gcctggaatt cggcgaatgc catgttgttc gacgccacta ccgtggcggc
    12061 ccggcccttc ttcttgatga aacgctcgac cggggccttg cgtttcgcag tcaccgacag
    12121 cgcaggtacg gtgcgcacgg cggaaacctc gacggcctat acctacgcgg caggcacctg
    12181 gcaccacatt gcagtgagct gggccttcac ccccggaacc aaccagaccg tgcagcagat
    12241 catgctggat ggcgtgctgg tcaacaccac aggcaccacg ccctatcgca ccacgtccag
    12301 tggcgccatc gccacgccca gtacgctgta catcggtgac aaccgaacct ctggcatcac
    12361 ccccagcacg ggctcgccca atggtgccaa cggcactatc gatgaggtct atgtctatgc
    12421 gctggatatc aatgcgaccc aggcggcggc cgacatggca ctcacacggc cgacgtgca
//
LOCUS       DAOSJM010000018        12389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5132_length_12389_cov_4.724501, whole genome shotgun sequence.
ACCESSION   DAOSJM010000018 DAOSJM010000000
VERSION     DAOSJM010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5132_length_12389_cov_4.724501"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="PKX82_01340"
     CDS             complement(<1..175)
                     /locus_tag="PKX82_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466276.1"
                     /note="oxidative demethylase of N1-methyladenine or
                     N3-methylcytosine DNA lesions; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HOF50918.1"
                     /translation="MDLFDDLPPLPEAVHTPIAPGAMLLRGFAREGDAALLQAVEAVI
                     AQAPLRHMQTPGGY"
     gene            complement(199..477)
                     /locus_tag="PKX82_01345"
     CDS             complement(199..477)
                     /locus_tag="PKX82_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50919.1"
                     /translation="MKYSVPFYIAAACATAALAGCVVAPVGMEPGYGGPGYGGPPGYG
                     GHGHAPPPAVVYVAPTYVMPGPGYLWQRHGRHGWGWHHPGRGWHRGWR"
     gene            660..1055
                     /locus_tag="PKX82_01350"
     CDS             660..1055
                     /locus_tag="PKX82_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOF50920.1"
                     /translation="MFSHVILGVNDLEASRKFYDALLGTLDIKPGVHNHNGVADRYFY
                     RTSTGSFGITTPINGEPATHGNGSTIGFTMASPEQADAFHAAGVANGGTTCEDPPGWR
                     DGSVGKLYLAYLRDPSGNKLCALFRPSKA"
     gene            complement(995..1654)
                     /locus_tag="PKX82_01355"
     CDS             complement(995..1654)
                     /locus_tag="PKX82_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HOF50921.1"
                     /translation="MKILPTDFDGQSIRRVYDEATETWWFSVVDVVQVLTQQADDLTA
                     RKYWNQLKRRLEKEGSQLVTNCHQLKMPAADGKQRLTDVATAQTLLRLVQSVPSPKAE
                     PIKLWLAKVGYERMQEMADPALSLNRARQTWQQHGRSEKWIAQRMTGQETRNKLTDYW
                     REHDVKGLSSHNLRDHMTEAEFILPLARVHRARAAINSIAYALDGRNSAQSLLPLGSR
                     R"
     gene            complement(1735..2427)
                     /locus_tag="PKX82_01360"
     CDS             complement(1735..2427)
                     /locus_tag="PKX82_01360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOF50922.1"
                     /translation="MVSFQRPDGKAVQGYLAEPATSANGANAPAIVVIQEWWGLNAQI
                     RGVADRLAAAGYQALVPDLYRGKATVEAEEAHHLMTGLDFGDAASQDIRGAVQFLKAR
                     APKVGLTGFCMGGALTLLALGQSPEIDAGVVWYGCPPLEYIDASKIRAPLMGHWATQD
                     EFFKIDAVDALEAKLHAAGVHPEFHRYLAHHAFANETAVGPGRIPATQYDPVWAQQAW
                     DRTLRFFGRHLG"
     gene            complement(2512..2964)
                     /locus_tag="PKX82_01365"
     CDS             complement(2512..2964)
                     /locus_tag="PKX82_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50923.1"
                     /translation="MNFRFFRLLRNIPPPVFILPTVALLLSAFMAWANAGAGDAFLPL
                     WARNFVTSLVVLPLILLGISQLERMLAFLAPNLRPFTAKLVVSLLTAVVIESVLAMVI
                     SLINSPWDATLSRYWWLAFSRSLPAGMAIGLFMGFYMKPRLDQMRRAT"
     gene            3035..3514
                     /locus_tag="PKX82_01370"
     CDS             3035..3514
                     /locus_tag="PKX82_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOF50924.1"
                     /translation="MTTSPSDASANSEAAVHTFVAFMTAFGGAQQVLKTRMQGHAEPG
                     LGPLHLRALYLCQRNPGAPQQQLVQALGRDKGQIARLIRDLEDHQLLVRTPDERDRRV
                     WRLTVTPEGEQKCGWFADMEAALARDLLGALKPKETAQLNAVLQTVQARIDAMGSNA"
     gene            complement(3550..3933)
                     /locus_tag="PKX82_01375"
     CDS             complement(3550..3933)
                     /locus_tag="PKX82_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013204825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF50925.1"
                     /translation="MAMSPKKQRGELFSVACPSRQVLQNVTSRWGVLVLVALLEGTYR
                     FSDLRRKVGGVSEKMLAQTLQALEADGFVLRTAHPVVPPHVEYSLTPMGTEVAREVDS
                     LADWIEGNLYRIMKQRNARSAASAH"
     gene            4023..4877
                     /locus_tag="PKX82_01380"
     CDS             4023..4877
                     /locus_tag="PKX82_01380"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF50926.1"
                     /translation="MIAVTGATGQLGRLVIQELLRSVPAAHIVAAVRTPAKAQDLAAL
                     GVQVRQADYAQPATLQAAFAGVDKLLLISSSEVGQRAPQHRAVIDAAKAAGVKLIAYT
                     SLLRADTSALGLAAEHKETEAALRASGVPFVLLRNGWYAENYAGTAPTAVQHGAVLGS
                     AKDGRIAAAARADYAAAAAAVLVREGQAGKVYELAGDTSFTLAEFAAEIAKQSGKPVA
                     YVDKPQADYAAALVKVGLPEGFAALLADSDAAAANGGLFDNGGQLSQLIGRPTTPIST
                     VIAAALKG"
     gene            4962..5711
                     /locus_tag="PKX82_01385"
     CDS             4962..5711
                     /locus_tag="PKX82_01385"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HOF50927.1"
                     /translation="MPAAQPHAITHAVFRLRAARLARSAKPFVARGSNSERCPGCRVV
                     FSHCLCMLRPVVPTTAGVCLIMFDIEPLKPTNTGWLIADVVPETFAFGWARTEVDPAL
                     LALLADPQWQPYVVFPGEFVDPARVVNTLPPALPAGKRPLFVLLDATWPEARKMFRKS
                     PFLNHLPVLSLQSEQLSRYRLRRSKRDDHFCTSEVAALCLELAGETHAAQTLEAYLDV
                     FTSHYLSAKRLVPADLDDAAHQRLRALAVAG"
     gene            complement(5694..6362)
                     /locus_tag="PKX82_01390"
     CDS             complement(5694..6362)
                     /locus_tag="PKX82_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOF50928.1"
                     /translation="MNTLTKMALGLIGQLKPTPLAFDPGQSIHLPAPTVVGGMPLMQA
                     LGARQSQRDFQPAPLPEQTLSDLLWAAAGINRAELGGRTAPSAMNAQEVDVYVALATG
                     LYRYAPKVHELHLVAAMDVRRVTGYQDFVDTAPLDLVYVADHGRMKTIPAARRESYAS
                     MTVGAMAQNVYLYCASAGLGAVVRAWFDQNALGQSMGLDADQQILLTQTVGLPAAAAS
                     ASHS"
     gene            6492..6743
                     /locus_tag="PKX82_01395"
     CDS             6492..6743
                     /locus_tag="PKX82_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50929.1"
                     /translation="MTDNYKAAAEAREAALCEPCRGIQRNWRRAPGHAELVQGDNRKE
                     DRNGRSVTVTRYRCDRCGSIWDYENDKANQQAGWSLPRR"
     gene            6766..7647
                     /locus_tag="PKX82_01400"
     CDS             6766..7647
                     /locus_tag="PKX82_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HOF50930.1"
                     /translation="MKAPPRLQSLIEEGLIDTVVRQLMSGKEAMVFVVRSGEQTLCAK
                     IYKEANNRSFRQAVDYTENRKVKNSRSARAMAKGSKFGRQEQEAAWQSAEVDALYRLA
                     AAGVRVPQPYNFHDGVLLMELVTDADGDAAPRLNDVAFTHAQALRHHATLIAEVVRML
                     CAGVVHGDLSEFNILLAHTPGVEGAEGVDEPVIIDLPQAVDAAGNNHAQRMLLRDVAN
                     LRDFFGQFAPELLRTDFGPEIWSLYQSGLLSNETVLTGRFERAHADVDMQAVLREIDD
                     ARAEDAARRLRMATASP"
     gene            7675..8379
                     /locus_tag="PKX82_01405"
     CDS             7675..8379
                     /locus_tag="PKX82_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOF50931.1"
                     /translation="MTDLSAYSVTRKWPAQHPERLQLYSLPTPNGVKVSILLEETGLP
                     YEAHLVSFDTNDQVSPEFVGTFPNNKIPAILDPNGPGGKPLALFESGAILLYLAEKTG
                     KLLPADPAARYETVQWLMWQMGGVGPMFGQLGFFHFFAGKDYDDKRPRDRYVAESKRL
                     LGVLDQRLATRSWIMGDEYTIADIAIFPWVRNLVERYGAGDLVGYADFTHVAHALAAF
                     VARPAVQRGLTIPNRS"
     gene            complement(8452..8937)
                     /locus_tag="PKX82_01410"
     CDS             complement(8452..8937)
                     /locus_tag="PKX82_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006496197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOF50932.1"
                     /translation="MAHDTPIPSEETDLVISRVLRAPRAKLWRAWTDPALLQEWWCPK
                     PWTTEVREFDMRPGGGFYTFMRGPDGGTSDNPGCFVDIVEQRRLVFTNALITGWRPAH
                     SWLTVTAVIEMADEGDGTRYVATAMHPDKATKDRHEEMGFFEGWNICIDQLDALACHL
                     P"
     gene            complement(9001..9537)
                     /locus_tag="PKX82_01415"
     CDS             complement(9001..9537)
                     /locus_tag="PKX82_01415"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOF50933.1"
                     /translation="MARRSFPLSKVYGLLEPGPVVLLSTAHKGKANVMTMSWHTMMEF
                     EPPLVGCVVSSRDFSFTALKATRQCVLGIPTAELAQAVVDCGNTSGRAIDKFAAFGLT
                     PVAASQVDAPLIDECYANLECRVVDTRLLNRYNFFVLEVVKAWTDPACKIPRTLHHRG
                     HGVFSVAGDTIKLPSKMK"
     gene            complement(9622..10620)
                     /gene="gap"
                     /locus_tag="PKX82_01420"
     CDS             complement(9622..10620)
                     /gene="gap"
                     /locus_tag="PKX82_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOF50934.1"
                     /translation="MTIKIGINGFGRIGRNVLRSAIQNFSDIEVVAINDLLEPDYLAY
                     MLQYDSVHGRFKGTVTVEGSNIIVNGKKIRLTQERDPSNLKWNEVGADIVIESTGLFL
                     DKASGEKHLAAGAKKVIFSAPSKDDTPMFVFGVNDKTYAGQAIISNASCTTNCLAPVA
                     KVLNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMSFRVPTSDVSVVDLTVELNKEASYEEICAEMKAQSEGALKGVLGYT
                     KDKVVATDFRGETCTSVFDADAGIALDKTFVKIVAWYDNEWGYSNKCLEMVRVVAK"
     gene            complement(10662..>11806)
                     /locus_tag="PKX82_01425"
     CDS             complement(10662..>11806)
                     /locus_tag="PKX82_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOF50935.1"
                     /translation="WKDKFAAYQAAYPALAKELLRRMKGDLPKNFVQTAVDTVIAAHT
                     KAETVASRKASQLALESFTAALPEMLGGSADLTGSNLTNTKATPALRVDLAGEEVVTP
                     EGKIGRHINYGVREFGMAAIMNGVALHGGYIPYGGTFLTFSDYSRNAIRMAALMKQRV
                     IHVFTHDSIGLGEDGPTHQSIEHAASLRLIPNLDVWRPGDTAETAVAWTVALQNSTKP
                     TALLLSRQNISYAPKAESALAPDACGLDAISKGAYVLAEPSEVGLKKKPQAVIIATGS
                     EVQLALHAQKVLAERKIAVRVVSMPSTTTFDKQAATYKASVLPAGLPRVAVEMGSTDG
                     WWKYGVSAVVGIDTYGESAPAPVLFKHFGFTPENVADTVQSVLAKK"
     assembly_gap    11807..11816
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11817..12389)
                     /locus_tag="PKX82_01430"
                     /pseudo
     CDS             complement(11817..12389)
                     /locus_tag="PKX82_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914542.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
BASE COUNT         2235 a         3862 c         3965 g         2317 t
ORIGIN      
        1 tgtagccgcc gggggtttgc atgtggcgca gcggtgcctg ggcgatgacg gcctcaacgg
       61 cctgcaacaa agctgcgtcg ccctcacgcg caaagccgcg cagcagcatg gcgccgggtg
      121 cgatgggtgt gtgcacagct tcgggcagcg ggggcaggtc gtcaaacagg tccatggctc
      181 cattctggag cagtgcgttt accgccagcc gcgatgccag ccccggcccg ggtggtgcca
      241 gccccagccg tggcggccgt gacgctgcca caagtagccc ggcccgggca tgacataggt
      301 gggggccaca tagaccacgg cggggggtgg ggcgtggccg tgaccgccgt accccggagg
      361 acccccataa cctgggccac catatcccgg ctccatgccg accggggcca ctacacaacc
      421 tgcaagggct gcggtggcgc acgctgcggc aatgtaaaag gggactgaat acttcatgcg
      481 gtggtccttg ttgggcaagc gtgcttgcga cccctgttca acgccagcca ctgcgcggag
      541 atgaccctcc aaggtaaagc ttgtgcaaac ctggcactca cacgtaaccc aataggcacc
      601 acgctgcgct gcctgcgcgc ttcgcgtacc atgccagcga ttcaaacaag gagacagcta
      661 tgttcagtca cgtgattctg ggtgtgaacg acctcgaagc ttcccgcaag ttctacgacg
      721 ccctgctggg cacactggat atcaaacccg gtgtgcataa ccacaacggt gtggcggatc
      781 ggtacttcta ccgcacgtcc accggctcgt tcggcatcac cacgcccatc aacggcgagc
      841 cagccacgca cggcaacggc agcaccatcg ggttcaccat ggcgtcgccc gagcaggcgg
      901 atgcgttcca tgcagcaggc gtggccaacg gtggcaccac ctgcgaagac ccacccggct
      961 ggcgcgatgg ctcggtcggc aagctgtacc tggcctacct gcgcgacccg agcggcaaca
     1021 agctctgcgc gctgttccgg ccatccaagg cataagctat tgaattgata gctgctcgcg
     1081 cacgatgcac ccgggccaga ggtaatatga attcggcttc ggtcatgtgg tcgcgtaggt
     1141 tgtggctgct caggcctttg acgtcatgtt cgcgccagta gtcggtgagt ttgttgcggg
     1201 tttcctgccc ggtcatgcgc tgggcaatcc acttctcgct acgaccgtgc tgctgccagg
     1261 tctggcgcgc acggttcagc gagagggccg ggtcagccat ctcctgcatg cgctcgtagc
     1321 ccactttggc gagccagagc ttgatgggtt cggctttcgg gctgggtacg gactgcacca
     1381 ggcgtagaag cgtttgggca gtagcgacat cggtgagacg ctgcttgcca tcggcggccg
     1441 gcattttcaa ctggtgacaa tttgtcacca gttggctacc ttccttttcc aatcggcgtt
     1501 tgagctgatt ccagtacttg cgtgcagtca aatcgtccgc ttgctgcgtc aacacttgca
     1561 ccacatccac gaccgagaac caccaggtct cggtcgcttc gtcatacacg cggcgtatgg
     1621 actgaccgtc aaagtctgtg ggcagaattt tcatgatgac cccctggagc aaccgtgttt
     1681 attggcacgg acaagaatac tgtattttta cacagtaatt tgtttgcatc cggttcagcc
     1741 caggtggcgg ccaaagaacc gcagcgtgcg gtcccacgcc tgctgcgccc acacggggtc
     1801 gtactgcgtg gcgggtatgc ggccggggcc gacggcggtt tcgttggcaa acgcgtggtg
     1861 ggccaggtag cggtggaact cggggtgcac gcccgcagcg tggagcttgg cttccagcgc
     1921 gtccaccgcg tcgatcttga agaactcgtc ctgcgtggcc cagtgaccca tgagcggcgc
     1981 gcggatcttg ctggcgtcga tgtattccag tggcgggcag ccgtaccaga ccacgccggc
     2041 gtcgatctcg ggtgactgac ccagcgccag cagcgtgagc gcgccgccca tgcaaaagcc
     2101 ggtcaggccc accttgggcg cgcgggcctt gaggaactgc accgcaccgc ggatgtcttg
     2161 ggatgcggca tcgccaaagt ccaggccggt catgaggtgg tgggcctctt cggcttccac
     2221 cgtggccttg ccgcggtaca ggtcgggcac cagcgcctgg tagccagcgg cagccagccg
     2281 gtcggccacg ccacggatct gcgcgttgag gccccaccat tcctgtatga cgacgatggc
     2341 cggggcgttg gcgccattgg ccgacgtggc cggctcggcc agatagccct gcacggcctt
     2401 gccgtcgggg cgctgaaaac tcaccatgtg tcccatgtct gctcctctgt gtggaatgaa
     2461 ctctggcgac attctggcct gttccgcgcc agggcgcatc cggggcaagc gctatgtggc
     2521 gcgacgcatc tggtcgaggc ggggcttcat gtagaagccc atgaacaggc cgatggccat
     2581 tcccgccggc agcgagcggc tgaacgccag ccaccagtag cgcgacaggg tggcgtccca
     2641 cgggctgttg atgagcgaga tgaccatggc cagcacgctt tcaatcacca ccgcggtcag
     2701 cagcgacacc accagcttgg cggtgaaggg gcgcaggttg ggcgccagaa atgccagcat
     2761 tcgctccagc tggctgatgc ccagcaggat cagtggcagc accaccaggc tggtcacaaa
     2821 gttgcgcgcc cacaggggaa gaaaggcgtc gccagccccg gcgttggccc aggccatgaa
     2881 ggcgctgagc agcagagcca cggtgggcaa gatgaaaacg ggtggcggaa tgttgcgaag
     2941 aaggcgaaag aagcgaaagt tcatggaggt tgtaaatagt tgacaatatc aacattgtag
     3001 aaattatggt tgataaagtc aaccatgtga tgccatgacc acatccccct ccgacgcctc
     3061 ggccaacagc gaagccgcag tccacacctt tgtggccttc atgacagcct ttggcggcgc
     3121 gcagcaggtg ctgaaaaccc gcatgcaggg ccatgccgaa ccgggcctgg ggccgctgca
     3181 cctgcgcgcc ttgtacctgt gccagcgcaa ccccggcgca ccgcagcagc aactggtgca
     3241 ggccctgggg cgcgacaagg ggcagattgc ccgcctgatc cgcgacctgg aagaccacca
     3301 gctgctggtg cgcacgcccg acgagcgcga ccgccgcgtg tggcgcctga ccgtgacgcc
     3361 tgaaggtgag cagaagtgcg gctggtttgc cgacatggag gcggcgctgg cgcgcgacct
     3421 gttgggtgcg ctcaaaccca aagaaaccgc gcagctcaat gccgtgttgc agacggttca
     3481 ggcccgcatc gacgccatgg gttcgaacgc ataagcctgc agcagggcgc tagccggccc
     3541 tacctggcgt tagtgggcac tggcagcgct gcgggcgttg cgctgcttca tgatgcggta
     3601 gaggttgccc tcgatccagt cggccaggct gtccacctcg cgcgccactt cggtccccat
     3661 gggggtgagg ctgtattcca catggggtgg caccacgggg tgggcggtgc gcagcacgaa
     3721 gccatcggcc tccagggcct gcagtgtctg ggccagcatt ttttcactca caccacccac
     3781 cttgcggcgc aggtcgctga agcggtaggt gccttccagc agggcgacca gcaccagcac
     3841 gccccagcgg ctggtgacgt tctgcagaac ctgacgggac gggcaggcga cggagaacaa
     3901 ctctccgcgc tgcttcttgg gtgacatggc catggggtac ttacctttat gtacgtactt
     3961 actaaaagtt tgcgccttgc ctattatcgc ggcctgtctg tttttttaca aggatgtatc
     4021 ccatgatcgc tgttaccgga gccactggcc aactgggccg tctcgttatt caggaactgc
     4081 tgcgcagcgt gccggcagcc cacatcgttg cggccgtgcg cacgcccgcc aaggcgcagg
     4141 acctggccgc cctgggcgtg caggtgcgcc aggccgacta tgcgcaaccc gccacactgc
     4201 aggccgcgtt tgctggtgtg gacaagctgc tgctgatttc ttccagcgaa gtgggccagc
     4261 gtgctccgca acaccgcgcc gtcattgacg cggccaaggc tgcaggcgtg aagctgattg
     4321 cctacaccag cctgctgcgg gcggacacat cggcactggg cctggctgcc gagcacaagg
     4381 aaaccgaagc ggcgctgcgc gcatcgggcg tgccgtttgt gctactgcgc aatggctggt
     4441 atgccgagaa ctatgcaggc accgcgccca ccgccgtgca gcacggtgcg gtactgggca
     4501 gcgccaaaga cgggcgcatt gccgccgccg cacgcgcaga ctacgcagcg gctgccgctg
     4561 ccgtgctggt gcgcgagggg caggctggca aggtgtacga gctggcgggc gacacgtcgt
     4621 tcaccctggc ggaatttgca gccgagattg ccaaacagtc gggcaagccc gtggcctatg
     4681 tggacaagcc gcaagccgac tatgcggccg cgctggtgaa ggtaggtctg cccgaaggct
     4741 ttgcagcgct gctggcagac tccgatgcgg cagcagccaa cggcggtctg tttgacaacg
     4801 gcggccagct gagccagttg atcgggcgcc ccaccacgcc catcagcacc gtgattgccg
     4861 ccgcgctgaa aggctaagcc gcggctgcag ctattgtttt tatagctggt tgcgcacatt
     4921 ccgcgggggc tacaggccag tttggtcaca tagaatcaga catgcctgct gcccagcccc
     4981 acgccataac tcatgcagtg ttccgcctgc gcgccgcccg tctggcgcgc agtgccaaac
     5041 cttttgtggc ccggggttcc aactccgagc gctgcccggg ctgccgcgtg gtgttcagcc
     5101 actgtctgtg catgctgcgc ccggtggtgc cgacgacggc gggcgtgtgc ctgatcatgt
     5161 tcgacatcga gccgctcaag ccgaccaaca cgggctggct gattgccgat gtggtgccgg
     5221 agacctttgc atttggctgg gcccgcaccg aagtagatcc cgcactgctg gccctgctgg
     5281 ccgacccgca gtggcagccc tatgtggtgt tccccggcga atttgtggac cctgcgcggg
     5341 tggtgaacac gttgccgcca gcgctgccgg cgggcaagcg ccctttattc gtgctgctcg
     5401 atgccacctg gcccgaggcg cgcaagatgt ttcgcaagag cccgttcctg aaccacctgc
     5461 cggtgctgag cctgcagagt gagcagctgt cacgctaccg cctgcgccgc tccaagcgcg
     5521 acgaccactt ctgcacctcc gaggtggcgg cgctgtgcct ggagctggcc ggtgagacgc
     5581 acgctgcgca aacgctggaa gcctacctgg acgtgtttac cagccactac ctgagtgcca
     5641 aacgcctggt gcccgccgac ctggacgacg cagcgcacca gcgcttgcgc gcgctagctg
     5701 tggctggctg aagccgccgc cgcgggcaac cccacggtct gggtcagcag gatctgctgg
     5761 tccgcatcca gtcccatgct ctgccccagt gcgttctggt caaaccaggc gcgcaccaca
     5821 gcccccagcc cggccgaagc gcaatacaga taaacgtttt gtgccatggc acccacagtc
     5881 atggacgcat acgattcgcg gcgcgcggcc ggaatcgtct tcatgcggcc gtggtcggcc
     5941 acgtacacca ggtccagcgg tgcggtgtcc acaaaatcct gatagccggt gacgcgccgc
     6001 acatccatgg ccgccaccag atgcagctcg tgcactttgg gcgcgtagcg gtacaggccg
     6061 gtggccaggg ccacatacac atcgacttcc tgcgcattca tggcactggg ggcggtgcgc
     6121 ccacccagtt ccgcacggtt gatgccggca gcggcccaca acagatcgga cagggtttgc
     6181 tcgggcaacg gcgcaggctg gaaatcacgc tgcgactggc gcgcaccaag tgcctgcatc
     6241 aggggcatgc cgccaactac cgtcggggca ggcaggtgga tggactggcc cggatcgaac
     6301 gcaagcggcg tgggtttgag ttgcccgatg agccccagag ccatttttgt cagcgtattc
     6361 ataaaccagc cctccttgtg gcgcgatgtt aggacgcgga tcgcgtgttg agttgacttt
     6421 tgtcagactt cgctcgcgcg gcgacaatca gacccttacc caaccgacgg cgctgccaga
     6481 gaaactcacc catgaccgac aactacaaag ccgccgcaga agcccgggaa gccgcgctat
     6541 gcgaaccctg ccgtggcatc cagcgcaact ggcgccgcgc ccccggccac gcagaactgg
     6601 tgcagggaga caaccgcaag gaagatcgca acggacgcag cgtgaccgtg acacgctacc
     6661 gctgcgaccg ctgtggctcc atctgggatt acgaaaacga caaagccaac cagcaagcgg
     6721 gctggtcgct gccgcgccgc tagacgtgga ccagcgcagc aggccatgaa agcacccccc
     6781 agactccaat ccctcattga agagggtctg atcgacaccg tggtgcgaca actcatgagc
     6841 ggcaaagaag ccatggtgtt tgtggtgcgc tcgggcgagc agacgctgtg cgccaaaatc
     6901 tataaggaag ccaacaatcg cagcttccgc caggccgtgg actacaccga aaaccgcaag
     6961 gtcaagaact cgcgctcggc ccgcgccatg gccaaaggca gcaagtttgg ccgccaggag
     7021 caggaagccg cgtggcaaag cgccgaggtg gacgcgctgt accgcctggc cgcggctggc
     7081 gtgcgcgtgc cacagcccta caactttcac gatggtgtgc tgctgatgga gctggtgacc
     7141 gacgccgatg gcgacgcagc gccacgtctg aacgatgtgg cctttaccca cgcgcaggca
     7201 ctgcgccacc acgccacgct gattgccgag gtggtgcgca tgctgtgcgc gggggtggtg
     7261 cacggggatt tgtcagagtt caacatcctg ctggcgcaca cgcctggggt cgagggtgcg
     7321 gagggtgtgg atgagccagt gatcatcgat ctgccgcagg cggtggatgc cgcgggcaac
     7381 aaccatgcgc agcgcatgct gctgcgcgat gtggccaacc tgcgcgactt ctttggccag
     7441 tttgctcctg agctgctccg caccgacttc ggccccgaga tctggagcct gtaccagagc
     7501 ggcctgctct ccaacgaaac ggtactgaca ggccgttttg agcgcgcgca tgccgatgtg
     7561 gacatgcagg ccgtgctgcg cgagattgac gatgcccgcg ccgaggacgc cgcgcgcagg
     7621 ctgcgcatgg cgacggcttc cccgtaaact ggtactgcaa ttcaggaacc tgccatgacc
     7681 gacctctctg cttattccgt cacccgcaaa tggcccgcgc agcacccgga gcgcctgcag
     7741 ctgtattcac tgcccacgcc caacggcgtg aaggtgtcca tcctgctgga agaaaccggc
     7801 ctgccgtatg aggcgcatct ggtgagcttc gacaccaacg accaggtgtc gcccgagttt
     7861 gtaggcacat ttcccaacaa caagattccc gccatcctcg accccaacgg gcctggtggc
     7921 aagccgctgg ccctgtttga atcgggcgcc atcctgctct acctggccga gaaaaccggc
     7981 aagctcctgc ccgccgaccc tgccgcgcgt tacgagacgg tgcagtggct gatgtggcag
     8041 atgggcgggg tggggccgat gttcgggcag ctgggtttct tccatttctt tgccggcaaa
     8101 gactatgacg acaagcgccc gcgtgaccgc tatgtggccg agagcaaacg cctgctgggc
     8161 gtactggacc agcgtctggc cacacgcagc tggatcatgg gtgacgagta caccatcgcg
     8221 gacattgcca tcttcccgtg ggtgcgcaac ctggtcgagc gttacggtgc aggtgatctg
     8281 gtgggctacg ccgacttcac gcatgtggcg cacgcactgg ccgcgtttgt ggcccggccg
     8341 gcagtacagc ggggcctgac cattccaaat cgctcctgat ttgatagctg cttgcgcaat
     8401 atctgcgagg gcttgctgcg tatttgatta ctaaagtcgc agcaggcacc gtcagggcaa
     8461 atggcaagcc agcgcatcga gctggtcgat gcagatgttc cagccttcga agaatcccat
     8521 ttcttcgtgg cggtctttgg tggccttgtc gggatgcatt gccgtggcca cgtagcgggt
     8581 gccatccccc tcgtcggcca tctcgatcac agccgtcacg gtgagccagg aatgtgcagg
     8641 acgccagccc gtgatcagcg cgttggtgaa gaccagcctg cgctgctcca cgatatcgac
     8701 aaaacagccc gggttgtcgc tggtgccgcc atcgggcccg cgcatgaagg tatagaaccc
     8761 gccgcccggg cgcatgtcga actcccgcac ttcagttgtc cacggtttgg ggcaccacca
     8821 ttcctggagc aaggccgggt cggtccatgc ccgccaaagt ttggcccgcg gtgcgcgcag
     8881 cacgcgggaa atgacaaggt cagtctcttc cgaggggatc ggggtgtcat gtgccatggg
     8941 atagtgcctc ctggaatgga gcgaacgata caccggacac ggccagtgtg tcttgccgcg
     9001 ttatttcatc ttggacggca gcttgatcgt gtcacccgcc acgctgaaca caccgtggcc
     9061 cctgtggtgc agagtgcgcg ggattttgca ggccgggtcg gtccaggcct tgaccacctc
     9121 cagcacaaag aagttgtagc ggttcaacag ccgcgtatcg accacccggc attccaggtt
     9181 ggcatagcac tcgtcaatca gcggcgcatc cacctgcgat gcggctactg gcgtcaggcc
     9241 aaatgccgcg aatttgtcga tggcgcgccc ggaggtgttg ccacagtcca ccaccgcctg
     9301 cgccagctct gcggtcggaa tgcccagcac gcattgccgc gttgctttca gtgccgtaaa
     9361 actgaaatca cggctactga ccacgcaacc caccagcggc ggctcgaact ccatcatggt
     9421 gtgccacgac atggtcatga cgttggcttt gcccttgtgt gcggtgctga gcaacaccac
     9481 cgggcccggc tccagcaggc catagacctt ggagagagga aaagaacgtc tggccatggg
     9541 caactcccga tttccggttc atgcgaaaaa aaggcccctg ccgttaccag cagaggcctt
     9601 tgcaatccgc gtcagggcga attacttggc gacgacgcgc accatttcca ggcacttgtt
     9661 ggagtagccc cactcgttgt cgtaccaggc cacgatcttg acgaaggtct tgtccagagc
     9721 gataccggca tcggcatcga acacgctggt gcaggtctcg ccgcggaagt cggtggccac
     9781 cactttgtcc ttggtgtaac ccagcacgcc cttcaaagcg ccttcgctct gcgccttcat
     9841 ttcagcgcag atttcttcgt aggacgcttc cttgttcagt tcaacggtca ggtcgaccac
     9901 ggacacatcg gacgtgggca cacggaagga catgccggtg agcttcttgt tcagttcagg
     9961 aatcactacg cccacggcct tggcagcgcc ggtgctggag gggatgatgt tttccagaat
    10021 gccgcggccg ccgcgccagt ctttgttgga cgggccgtcc acggtcttct gggttgccgt
    10081 ggctgcgtgc acggtggtca tcaggccgcg cttgatgccc cacttgtcgt tgagtacctt
    10141 ggccacggga gccaggcagt tggtggtgca ggatgcgttg gagatgatgg cctggccagc
    10201 gtaggtcttg tcgttcacgc cgaacacgaa catgggggtg tcgtctttgg aaggcgcaga
    10261 gaagatgact ttcttggcgc cagcagccag gtgtttttca ccggaggctt tgtccaggaa
    10321 caggccggtg gactcgatca cgatgtcggc accgacttcg ttccacttca ggttggaagg
    10381 atcgcgctct tgcgtcaggc ggatcttctt gccgttgacg atgatgttgc tgccttccac
    10441 ggtcacggtg cccttgaagc ggccgtgcac gctgtcgtac tgcagcatgt acgccaggta
    10501 gtcgggctcc agcaggtcgt tgatggcaac gacttcgatg tcagagaaat tttgaattgc
    10561 ggagcgcaac acgttgcgtc cgatgcgtcc gaagccattg atgccgatct tgatagtcat
    10621 ggtgatttcc ttaaaaaata atctgccaaa aatagagctg cttacttctt tgccagcacg
    10681 gattgcaccg tatcggccac attctcggga gtgaatccaa agtgcttgaa cagcaccggt
    10741 gcgggagcgg actcaccgta ggtgtcgatg cccaccacgg ccgacacgcc gtatttccac
    10801 cagccgtcgg tcgaacccat ttcgaccgcc acgcggggca accctgccgg cagcacggaa
    10861 gccttgtagg tcgcggcctg tttgtcgaag gtggtggtgc tgggcatgga gaccacgcgc
    10921 accgcaatct tgcgctcggc cagaaccttc tgcgcatgca gggccagttg cacttcggaa
    10981 cccgtggcga tgatgaccgc ctggggcttc ttcttcaggc ccacttcgga aggctcggcc
    11041 agcacatagg cgcccttgct gatggcatcc agaccacacg catcaggagc cagtgcagac
    11101 tcggccttgg gggcgtagct gatgttctgg cgggacagca acagcgcagt gggcttggtg
    11161 ctgttctgca gggcaacggt ccaggccaca gccgtttcag ccgtatcgcc cggacgccat
    11221 acgtccagat tgggaatcag gcgcaggctt gctgcatgct cgatggactg gtgggtgggg
    11281 ccgtcttcgc ccagaccgat ggagtcgtgg gtgaacacgt ggatcacgcg ctgcttcatc
    11341 agcgcggcca tgcggatggc gttgcggctg tagtcgctga aggtcaggaa ggtgccgccg
    11401 taggggatgt agccgccgtg cagcgccacg ccgttcatga tggcggccat gccgaattcg
    11461 cgcacgccgt agttgatgtg gcggccgatc ttgccttcag gtgtcacgac ctcctcgccc
    11521 gccaggtcca cgcgcaacgc gggggtggcc ttggtgttgg tcaggttgga gccggtcagg
    11581 tcggccgagc cgcccagcat ttcgggcagg gcggcggtga aagattccag cgccagttgc
    11641 gatgccttgc ggctggcaac cgtctcggcc ttggtgtggg cagcgatgac ggtgtccacg
    11701 gcggtctgca cgaagttctt gggcaggtcg cccttcatgc ggcgcagcag ctctttggcc
    11761 agagcggggt aggcggcctg gtaggcagcg aacttgtctt tccaggnnnn nnnnnncctt
    11821 ttccttggca tcccagtcgg catacacctc cttgggaatc acgaagggtg catggctcca
    11881 gccaatggat tcacgtgtga gcttgatctc ttcggcaccc agaggttcgc cgtgggcctt
    11941 ggcggtgttg gcgcggttgg ggctgccttt gccgatgtgg gtcttgcaga tgatgagcgt
    12001 ggggagttcg ctggacttct tggcctcggc aattgcagcg gacacggcgg tggcgtcatg
    12061 gccgtcgatg gggccgatca cgttccagcc gtaggccaca aaacgctgcg cggtgttgtc
    12121 gatgaaccag ggcgcgacct gaccgtcgat ggagatgccg ttgtcgtcgt acagggcaat
    12181 gagcttgctt aagcgccagg cgccagccag tgcggccgct tcgtggctga tgccttccat
    12241 caggcagccg tcgcccatga acacataggt gtggtggtcc accaccggat ggccgtcgcg
    12301 gttgaattcc ttcgccagca gcttttcggc cagcgccatg cccaccgcgt tggtgatgcc
    12361 ctggcccagc gggccggtgg tggtttcca
//
LOCUS       DAOSJM010000019        12194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5244_length_12194_cov_4.781778, whole genome shotgun sequence.
ACCESSION   DAOSJM010000019 DAOSJM010000000
VERSION     DAOSJM010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12194
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5244_length_12194_cov_4.781778"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /gene="dksA"
                     /locus_tag="PKX82_01435"
     CDS             <1..599
                     /gene="dksA"
                     /locus_tag="PKX82_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOF50936.1"
                     /translation="KAGRPSSRLAQLTVPSMAQSVASTAAKASYSQSTPSPVIVPALP
                     SSIKKDPKLANNWKTKPVAELTDAELMAMPDSEYMNDVQMAAFRLKLSKLKQDILNNA
                     GETTEHLREDTSVVPDPADRATIEEEHALELRTRDRERKLLKKIEQSIVRIDAGDYGY
                     CDETGEPIGVGRLLARPTATLSLEAQQRRELKQKMFGD"
     gene            641..2374
                     /locus_tag="PKX82_01440"
     CDS             641..2374
                     /locus_tag="PKX82_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOF50937.1"
                     /translation="MATKDDRPGLLSKVAKFVRNPTKDWSELDNPDQPQESGYDKQAL
                     KAMIERKRQNDFVRKREFDQLRKLRNRDPAAMANMARPSFFQSSVATDQDGRAVTLKK
                     IDEIEAQMSKQWWKGKQEGGTRAGDVAVAQISRFSGDSLSQGASQFPTQAPTQSPHHF
                     APTEAVGLQALPEGPADFEPTNVPLGHVPAPGRIAHADSSHAFSGEDQSFSTSELFAM
                     EVDDMSTDPELEEAAIRFANGDDTGAEKGLLEALRGEVLEPEVALSWAAALLDLYRVT
                     HKRPQFDSAVKEFGARFGRMVPVWASLGGDGASSESSQGAFGAASAVSSTEHGVIWQS
                     PSMLDARAMEALRDALSSHPTPWHVGWGTLEGIAVDAVPLLDALFVSLCDEPVALRFS
                     GAHNLLERLRALTPSGDRAVDRAWWHVRLNALRSMNLIDEFELAALDYCVTYEVSPPA
                     WVEARCQFEATEAAPAHRGAAAAPTGAIPTQSSAAGDIPELRGEVLGDATQLLGALVK
                     GAQGSGLLVVSCANLVRVDFSAAGSILNWATMRQAEGGQVQFKDVHRLVAAFFSVIGI
                     NEHARVIPRPI"
     gene            2440..2988
                     /gene="hslV"
                     /locus_tag="PKX82_01445"
     CDS             2440..2988
                     /gene="hslV"
                     /locus_tag="PKX82_01445"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOF50938.1"
                     /translation="MEQFHGTTIISVRRKTPEGMQVAIGGDGQVTLGNIVVKGTARKV
                     RKLYHGKVLAGFAGATADAFTLFERFEAKLEKHQGHLVRAAIELTKDWRTDRVLRRLE
                     AMLAVADKDASLIITGNGDVLEPENGIVTIGSGGAYAQAAAIALLNNTELGAADIVKR
                     SLEIAGELCIYTNMQHTIETLD"
     gene            2997..4322
                     /gene="hslU"
                     /locus_tag="PKX82_01450"
     CDS             2997..4322
                     /gene="hslU"
                     /locus_tag="PKX82_01450"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOF50939.1"
                     /translation="MTPQDIVTELDRHIVGQAQAKRAVAIALRNRWRRQQVEAGLRAE
                     ITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGKDVDSIIRDLAE
                     IAVKQTREAAKTQVRARAEDAAEERILDILIPPPRTEFGMASSVETPESTARQTFRKK
                     LREGQLADREIELDVAVSAPQMEVMGPAGMEEMTEQLRGMFGQLGQARKQTRKLKISE
                     AQKLLADEEAAKLVNEDEIKTQALQMLEQNGIVFIDEIDKVTSRSEGSGAEVSRQGVQ
                     RDLLPLVEGTTVSTKYGMVKTDHILFVASGAFHLSKPSDLIPELQGRFPIRVELQSLS
                     VEDFEAILTQTHASLVKQYQALLATEGVTLEFTPEGITRLAQIAYDVNERTENIGARR
                     LATVMERLLDDVSFDAAAMAEQTVRIDAPYVDVRLAELSQNEDLSRYIL"
     gene            4572..5000
                     /gene="mraZ"
                     /locus_tag="PKX82_01455"
     CDS             4572..5000
                     /gene="mraZ"
                     /locus_tag="PKX82_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOF50940.1"
                     /translation="MFQGASSLSLDAKGRLSVPTRHRDVLSATANGHLTITKHPHGCL
                     MVFPRPEWEKFRERIAALPMSAQWWKRIFLGNAMDVEMDATGRVLVSPELRAAAGISK
                     DTMLLGMGNHFELWDKATYDAQEAKAMQGDMPDVFKDFSF"
     gene            5010..5945
                     /gene="rsmH"
                     /locus_tag="PKX82_01460"
     CDS             5010..5945
                     /gene="rsmH"
                     /locus_tag="PKX82_01460"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOF50941.1"
                     /translation="METTWNHATVLLNEAVDALFTDGANNFADAADGTYVDATFGRGG
                     HSRLILLRLSPQGRLIAFDKDPQAVAESAAIADPRFSIRHEGFAHLGDLPAKSVTGLL
                     MDLGVSSPQIDNPARGFSFRFEGPLDMRMDTTRGESVAQWLATAEVNQITEVIREYGE
                     ERFAGSIAKAIVARRQERGAISTTTELAQLVADTVKTREPGKDPATRTFQAFRIFINA
                     ELEELQQALEASLDVLQPGGRLAVISFHSLEDRIVKQFIAKHSRDEYDRRAPFAAPKV
                     MKLESLGRIKPSDDEVRGNPRSRSAIMRVARRTAA"
     gene            5942..6244
                     /gene="ftsL"
                     /locus_tag="PKX82_01465"
     CDS             5942..6244
                     /gene="ftsL"
                     /locus_tag="PKX82_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOF50942.1"
                     /translation="MMRLNLVLLLAVLASAVYLVNVQYESRRVFTELDRARSEARRLE
                     TEYERLQVEKRGQATPARVEKLAREKLQMRQATPAITTYVTYSAPAGEDVAAGVAP"
     gene            6241..7974
                     /locus_tag="PKX82_01470"
     CDS             6241..7974
                     /locus_tag="PKX82_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOF50943.1"
                     /translation="MSRSIQYTSSPLLASRTPVWRSKFVVGAIALAFFGLAARAAYIQ
                     VVANDFFQRQGVVRFERTLDLPANRGRILDRNGLILASSVPAPSIWAIPEDVDLDKAQ
                     LQRLAKMLDMPVSDLAKKLRDEDKTFVWIKRQVDAGIAKQIAEMNLKGIYQRKEYKRE
                     YPEGESAVHVVGFTNVEDNGQEGVELTFNKDLAGKSGSRRVIKDRLGRVVEDVGDQIP
                     PVEGRDLQLSIDSKVQFFAYQKLREAVVENKAQAGSVVVLDVLTGEVLALANYPSYSP
                     AKRSNLTGAQLRNRALTDTFEPGSTMKPFSIALALEKGLVKPESIIQTAPGKITISGS
                     TITDAHPHGALTVNEVIQKSSNVGTVKLAMQMQPHEMWEMFSQVGLGQKPQVPFPGVV
                     SGRLRAYKTWRPIEQATMSYGYGLSASLFQLARAYSVFARDGSLVPVTLLKTAQQVEG
                     VKVFEPRHAREVLHMLYLAAGPGGTAPKAQTMGYSVGGKTGTAHKQEGKGYAAKKYRG
                     FFVGVAPIDKPRIVVAVMIDEPSNGKYFGGDVAAPVFAETVQQTLRMLGVQPDMAVRP
                     QIVANAVEESF"
     gene            7974..9470
                     /locus_tag="PKX82_01475"
     CDS             7974..9470
                     /locus_tag="PKX82_01475"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOF50944.1"
                     /translation="MQELHTPVQAAQWLKSRACGALWADSRKVRPGDGFVAWPGAAVD
                     ARGFAATAIAQGAGACLMERDGAEAFGLSGESYATYAGLKADTGLIAAEFYGQPSRAL
                     DVVAVTGTNGKTSTAWWLAQALKNLPAERSWPCGVIGTLGVGIPPDVLSTGLTTPDPV
                     LLQQTLRAFVDTGLKACAMEASSIGVEEHRLTGTDIRVAVFTNFTQDHLDYHGSMASY
                     WDAKRRLFVWPGLQAAVINVDDTQGAQLAADLANGPLDVWTTSCSQAARLRADNIQYA
                     TRGLRFDVVEGGQTIAFHSHLIGTYNVANMLGVIAAMCSLGVPLADAVQACSGLMPVP
                     GRMECVGNAGEPLVAVDYAHTPDALEQALVALRPLAQQREGALWCIFGCGGDRDASKR
                     PLMGAIAAKCADRVVVTSDNPRSEKPEAIIAQILLGLPQTDMVSVQADRAQAIARTLA
                     AVADQDVVLLAGKGHEATQEVAGSKLPFSDRAHADQALQARHSGGVRP"
     gene            9476..10876
                     /gene="murF"
                     /locus_tag="PKX82_01480"
     CDS             9476..10876
                     /gene="murF"
                     /locus_tag="PKX82_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOF50945.1"
                     /translation="MMTLHQATQWMPGARLVGENKVVVLRVHTDTRTVEPGDLFVALQ
                     GERYDANEFLQEAQQRGAVGVVCHAGLRADLYPAGLPRIEVADTKAALQSLATTWRAR
                     FTLPVIAVTGSNGKTTVTQMLAAILDAQCPGGGALATRGNFNNDIGVPLTLLRLRAGH
                     RMAVVELGMNHPGEIATLAAMAQPTVALVNNAQREHLEFMQTVQAVARENGSVIASLP
                     ADGVAVVPADDEFTALWQDLAAPRRCVRFALSGGDSQDEMHCVETHWEHGGWQVRAQG
                     MGAHLHYRLSIAGRHNVKNSLAAAACALAAGVSASAVEQGLQAFQPVKGRSRALEIAV
                     QGRERTLVDDTYNANPDSVRAAIDVLADLPGPRILVLGDMGEVGHEGPAFHAEAGRYA
                     LAKGIDQLLAHGNLVRSAVDVFPGARHFESMDELNDAVLAGLPHASSVLVKGSRFMRM
                     ERVVEAVVAHFSNGEP"
     gene            10876..12054
                     /gene="mraY"
                     /locus_tag="PKX82_01485"
     CDS             10876..12054
                     /gene="mraY"
                     /locus_tag="PKX82_01485"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOF50946.1"
                     /translation="MLLSLAQWLQTLSPELGFFRVFQYLTFRAVMAAMTALLIGLAAG
                     PAVIRRLRELKIGQPIRGYGMESHLSKSGTPTMGGVLILLSIAISTLLWFDLSNRFVW
                     IVLLVTLGFGAIGWADDWRKVVHKDPEGMRSREKYFWQSVVGLLAALYLVFSISESSN
                     LRVLELFFNWVRSGFDVNLPPKAGLQLPFFKEVSYPLGVLGFVVLTYLVIVGSSNAVN
                     LTDGLDGLAIMPVVMVGSALGVFAYVTGSVVYSKYLFFPYIPGSGELLIFCSAMAGAG
                     LAFLWFNTHPAQVFMGDVGALALGGALGTIAVIVRQEIVLAIMGGIFVVEALSVMLQV
                     SYFKYTKKKFGEGRRLLKMAPLHHHFEKSGWKETQVVVRFWIITMLLCLVGLSTLKLR
                     "
BASE COUNT         2389 a         3460 c         3888 g         2457 t
ORIGIN      
        1 aaaaggcggg gcgtccttcg tcccgtctcg cccagctgac cgtgccttcc atggcccagt
       61 cggtggcgtc gaccgctgcc aaagccagtt atagccaatc gactccctct cccgtgattg
      121 ttcctgcctt gccttcctcg atcaaaaaag accccaaact cgccaacaac tggaagacca
      181 agccggttgc ggagctgacg gacgccgaac tgatggcgat gcctgattct gagtacatga
      241 acgatgtcca gatggcagca ttccgtctca agctgtccaa gctcaagcag gacatcctga
      301 acaatgccgg tgagaccact gaacacctgc gcgaagacac ttcggtggtg cccgaccccg
      361 ctgatcgggc caccattgaa gaagagcatg cgctcgaatt gcgtacccgt gaccgtgagc
      421 gcaagctgct caagaagatt gaacagtcca tcgtccgcat tgatgcgggt gactatgggt
      481 actgcgacga aaccggggaa cctatcggcg taggtcgcct gctggcccgt cccaccgcaa
      541 ccttgtcact ggaagcccag cagcgccgcg aactcaagca gaagatgttc ggggattgat
      601 tcccggcgct agataggtag ccctgcgcta aactgttccc atggccacta aagacgatcg
      661 ccccggtttg ctgtcgaagg tggcgaagtt cgtccgcaat ccgaccaaag actggtcgga
      721 gctggacaac ccggaccagc cgcaggaaag cggctatgac aagcaggccc tcaaggccat
      781 gattgagcgc aagcggcaaa acgactttgt ccgcaagcgc gaatttgacc agttgcgcaa
      841 gctgcgcaac cgggatccag cggccatggc caacatggca cgcccgtcct tcttccagtc
      901 cagtgtggct accgaccaag atggtcgtgc cgtcacgctg aagaagatcg acgagatcga
      961 agcccaaatg tccaagcaat ggtggaaggg caagcaggag gggggaaccc gtgcgggcga
     1021 tgtcgccgtt gcgcaaattt cccgcttctc gggcgacagt ctgagccagg gcgccagtca
     1081 gttccctacc caggcaccta cccagtcccc ccaccatttc gcgccgaccg aggccgtggg
     1141 tttgcaggcg ctgcctgaag gtcctgcgga ttttgagcca accaatgtgc cactgggcca
     1201 cgtgccggcg cccggacgga tagcccacgc tgattcctct catgctttct cgggagaaga
     1261 ccagagtttt tccacctccg aactgtttgc gatggaggtc gacgatatgt cgaccgatcc
     1321 tgaactggaa gaggcagcca tccgctttgc caacggggac gataccggag ccgaaaaggg
     1381 gctgctggaa gcgttgcggg gagaagttct ggagccagag gtggcgctgt cctgggcagc
     1441 ggcattgctg gatttgtacc gggtaaccca taagcgcccc caatttgaca gtgccgtcaa
     1501 ggagtttggt gcgcgtttcg gccggatggt gcccgtgtgg gcatccctcg ggggagatgg
     1561 agcatcctcc gaatcctcgc aaggggcgtt tggggccgct tcagcggttt cgtctactga
     1621 gcacggggtg atctggcaaa gtccgtccat gctggatgcc cgcgccatgg aagctctacg
     1681 cgacgccttg tcatcgcatc cgacgccctg gcatgtgggg tggggcactc tggaggggat
     1741 tgccgtcgat gcggtcccct tgctggatgc actatttgtc agtctgtgtg atgaaccggt
     1801 tgcactgcgc ttctctggcg cccacaacct cctggagcgg ctgcgggctc tgacgccatc
     1861 gggagaccgt gcggtggacc gggcttggtg gcatgttcgc ctcaatgctt tgcgctccat
     1921 gaatctcatc gatgagttcg agttggcggc attggactac tgcgtgactt acgaggtatc
     1981 ccctcctgcg tgggtggagg cgcgatgcca gttcgaagcg accgaggcag cgccagcgca
     2041 ccgtggcgca gcggccgccc ctaccggcgc cattccgacg cagtccagcg cagcagggga
     2101 tattcctgaa ttgcgcggtg aagtgctggg tgatgccaca caattgctgg gtgcgctggt
     2161 caaaggtgcg caaggcagtg gtctcctggt cgtgtcatgt gccaatttgg tccgggtgga
     2221 tttttctgct gcggggagta tcctgaattg ggcgacgatg cggcaggcag aaggtggaca
     2281 ggtgcagttc aaggacgtgc accgtctggt cgccgccttc ttcagcgtca tcggtatcaa
     2341 cgagcatgcc cgggtgatcc ccaggcccat ttgatcgtcg ggcacgcgtg tgccccaagt
     2401 ctctcttgca atccgggtta tcgcccgaat ctcaatccca tggaacaatt tcacggcacc
     2461 accatcatca gcgttcgtcg caagacgcca gaaggcatgc aggtcgccat tggcggcgat
     2521 ggtcaggtta cgctgggcaa tatcgtcgtc aaaggcacgg cacgcaaagt gcgcaagctc
     2581 taccacggca aagtgctggc gggtttcgcc ggtgcaacgg cagatgcctt cacgctgttc
     2641 gagcggtttg aggccaagct ggaaaaacac cagggacacc tggtgcgcgc cgccatcgag
     2701 ctgaccaagg actggcgcac cgaccgcgta ttgcgccgcc tggaagccat gctggcggta
     2761 gcggacaagg atgcctccct gatcattacg ggcaacggtg atgtgctgga gccggaaaac
     2821 ggcattgtga ccattggttc gggtggcgcg tacgcgcagg cggcggctat cgctttgttg
     2881 aacaacaccg agttgggtgc ggcagacatc gtcaaacgct cgctcgaaat cgctggcgag
     2941 ctgtgcattt ataccaatat gcagcacacc atcgaaactc tggattgaaa actgtcatga
     3001 caccacagga catcgtcact gaactcgatc gccacattgt gggccaggcc caggccaagc
     3061 gtgcggttgc cattgcattg cgcaaccgct ggcgccgcca gcaggtggag gccggactgc
     3121 gtgccgaaat cacgcccaag aacattctca tgatcggccc tacgggggtc ggcaagacgg
     3181 aaattgcacg gcgcctcgca cggctggcag atgcaccttt catcaaggtc gaggcgacca
     3241 aattcaccga ggtcggctac gttggcaagg acgtggactc catcatccgt gacctggcag
     3301 aaatcgccgt gaagcaaacg cgtgaggcgg caaagaccca ggtgcgtgcg cgcgccgaag
     3361 acgctgccga agagcggatt cttgacatcc tgatcccgcc gccccggact gagttcggaa
     3421 tggcgtcctc ggtggagaca cccgaaagca cggcgcgaca aactttccgc aagaaactgc
     3481 gcgagggcca gctggccgac cgcgaaatcg agctggatgt ggcagtgtcc gcgccacaaa
     3541 tggaggtgat ggggcctgcg ggcatggagg agatgaccga acagctccgc ggcatgtttg
     3601 gacagttggg gcaggcccgc aaacagacgc gcaagctcaa gatcagtgaa gcacagaagc
     3661 tgctggccga cgaagaggcg gccaagctcg ttaacgagga cgagatcaag acccaggcac
     3721 tgcagatgct ggagcaaaac ggcattgtgt ttattgatga gatcgacaag gtcacatcgc
     3781 gcagcgaggg cagtggtgcc gaagtttcgc gccagggtgt gcagcgcgac ctgttgccgc
     3841 tggtcgaggg caccacggta tccaccaagt acggcatggt caaaacagac cacatcctgt
     3901 ttgtcgcctc cggtgcattt cacctgagca aaccgagtga ccttattccg gaactgcagg
     3961 ggcgttttcc gattcgggtg gagttgcagt cattgtccgt tgaggacttt gaagcgatcc
     4021 tgacccagac ccatgcgtca ttggtgaagc aataccaggc cttgctggcc accgagggtg
     4081 tgaccctcga gttcaccccg gaaggcatta cccggctggc ccagattgcc tatgacgtga
     4141 acgagcgtac cgagaatatc ggggcgcgac gattggcgac cgtcatggag cggttgctgg
     4201 acgatgtgag cttcgatgcg gcggctatgg cggagcaaac ggtgcgtatt gacgcaccct
     4261 acgtcgacgt gcgtctggcc gaactcagcc aaaacgaaga tttgtcacgc tacatcctgt
     4321 aaagggcggt ttgttacagg gcttcaagca tcactttcat aaagcgtgct aagtccttca
     4381 tttagaacga tttttggctc ccatttgcct tcggagtcgc gtctaagtcc ttgatttcat
     4441 tggaaaaaat tctcgcaagc cctcttgcaa gccttgcaat tgctgataca gtgcaaaaaa
     4501 gtgcaattaa gtggtgaaaa gtgcctctga gttgctattt cgagctgttg cacaacaaaa
     4561 gggtgtctgt cgtgtttcaa ggggcttcgt ctctcagtct ggatgcaaaa ggtcggctct
     4621 ctgtgccgac ccggcaccgt gacgtgctga gcgccaccgc gaacggccac ctgaccatca
     4681 ccaagcatcc gcacgggtgc ctgatggtgt ttccccgccc cgagtgggaa aaattccgcg
     4741 agcgtattgc cgccctgccc atgtcggcac agtggtggaa gcggatcttc ctgggtaacg
     4801 ccatggatgt ggagatggat gccaccggcc gggtgctggt gtcgcccgag ttgcgcgctg
     4861 ccgcaggcat ctccaaagac acgatgttgc tgggcatggg caaccatttc gagctctggg
     4921 acaaggcgac ctacgacgcc caggaagcga aggccatgca gggcgacatg cccgatgtct
     4981 tcaaggattt ttcgttctag gcgattgcgg tggaaacaac atggaaccat gccaccgtct
     5041 tgttgaacga agcggtcgat gcattgttca cagacggggc caacaacttt gccgatgcgg
     5101 ccgatggcac ctatgtggat gccaccttcg ggcgtggcgg ccactcccgt ttgattcttt
     5161 tgcgtttgtc gcctcagggg cgattgattg ccttcgacaa agatccccag gcggtggccg
     5221 aatccgcagc catagccgac ccccgctttt ccattcgcca cgaaggcttc gcccacctgg
     5281 gcgatttgcc cgcgaaaagc gtgaccggcc tgttgatgga tttgggtgtg agttcacccc
     5341 agatcgacaa ccccgcacgg ggattcagtt ttcgtttcga aggtccgctg gatatgcgca
     5401 tggataccac gcgcggagag agtgtggcgc aatggctggc aacagcagaa gtcaatcaaa
     5461 tcacggaggt gatacgtgaa tatggtgaag aacggtttgc tgggtcaatt gcaaaggcga
     5521 ttgttgctcg ccgacaggaa cggggcgcaa tttcaaccac caccgagttg gcccaactcg
     5581 tggctgacac ggtcaaaacc cgcgagccgg gcaaggaccc tgcaacgcgc acatttcagg
     5641 cttttcggat tttcatcaac gccgagcttg aagagctgca acaggcgcta gaggccagtc
     5701 tggatgtgct gcagcccgga ggccgtcttg ctgtgatcag ctttcattcg ctggaagacc
     5761 ggatcgtcaa gcaattcatc gcgaagcatt cgcgcgatga gtacgaccgt cgcgccccgt
     5821 ttgcagcgcc caaggtcatg aagcttgagt ccctggggcg tatcaagccc agcgatgacg
     5881 aggtgcgcgg caatccgcgc tcacgtagcg cgatcatgcg tgtggcgcgg aggacggcag
     5941 catgatgcgc ctcaacctcg tgctgttgct tgccgttttg gccagtgccg tttacctggt
     6001 caatgtgcag tacgagtcac ggcgggtgtt caccgagctt gacagggcgc gctcggaggc
     6061 gcgccgcctg gagacagaat acgagcgctt gcaggtggag aaacgtgggc aggccacgcc
     6121 cgcccgggtg gaaaaactgg cgcgggagaa gttgcagatg cgccaggcga cacctgccat
     6181 cactacctac gtgacctatt cggctcccgc aggcgaagac gtcgcagccg gagttgcgcc
     6241 gtgagccgca gcatccagta cacctccagc cccctgctgg caagccgcac gccggtgtgg
     6301 cgcagcaagt ttgtggtggg ggcgattgca ctggccttct tcgggctggc tgcgcgggcc
     6361 gcgtatatcc aggtggttgc caacgatttc tttcagcgcc agggcgtggt ccgcttcgag
     6421 cggacgctgg atttgccggc caaccgtggg cgcatcctgg atcgcaatgg actgatcctt
     6481 gcctccagcg tgccagcgcc cagtatctgg gccattcccg aagacgtcga tctggacaag
     6541 gcacaactgc agcgtctggc gaagatgctg gatatgcctg tgagcgacct ggccaagaaa
     6601 cttcgggacg aagacaagac ttttgtctgg atcaagcgcc aggtggacgc tggcatcgct
     6661 aagcagatcg cggagatgaa tctcaaggga atctaccagc gcaaggaata caagcgcgag
     6721 tacccggaag gcgagtctgc ggttcatgtc gtgggcttta ccaatgtgga agacaacggc
     6781 caggaagggg tggaactcac cttcaacaag gatctggcgg gcaagagcgg ttcgcgccgc
     6841 gtcatcaagg atcgcctggg gcgtgtggtg gaagatgtgg gcgaccagat tcctcccgtg
     6901 gaggggcgcg acctgcaact gagcatcgac agcaaggtac agttctttgc ctaccagaag
     6961 ctgcgcgaag cagtggtgga gaacaaggcg caggcaggca gcgtggtggt tctggatgtg
     7021 ctgaccgggg aggtcctggc gcttgccaac taccccagct attcgcccgc caaacgctcc
     7081 aacctgacgg gggcgcaact gcgcaaccgt gcgctgaccg acacatttga gccaggctcc
     7141 accatgaagc cgttttccat cgcactggca ttggagaaag gactggtcaa accggaaagc
     7201 atcatccaga cggccccagg gaaaatcacc atttccggct ctacgatcac cgatgcgcac
     7261 ccgcatgggg ccttgaccgt caatgaggtc atccagaagt ccagcaatgt gggtacggtc
     7321 aagctggcga tgcagatgca gccgcacgaa atgtgggaaa tgttcagcca ggtggggctt
     7381 ggacaaaagc cgcaggtgcc atttcctggc gtggtcagtg gtcgcctgcg tgcctataaa
     7441 acctggcgtc ccattgaaca ggcgactatg agttacggct atggcctgtc ggccagcctg
     7501 ttccagttgg cgcgtgcgta cagcgtgttt gcgcgggatg gctctctggt tcctgtcact
     7561 ttgctaaaga ccgcccagca ggtggaaggt gtcaaagtgt ttgagccacg ccatgcacgg
     7621 gaagtactcc atatgctgta tctggctgca ggccccggtg gtacggcacc caaagcccag
     7681 accatgggct actcggttgg cggaaaaacc ggcactgccc acaagcagga aggaaagggt
     7741 tatgccgcca aaaagtaccg cggctttttt gtcggagtgg cacctattga taagccgcgc
     7801 attgttgtag ctgtgatgat tgatgagccc agcaacggca agtattttgg cggcgatgtg
     7861 gccgcgccgg tgtttgccga gacggtgcag caaacattgc gcatgctggg cgttcaaccc
     7921 gacatggctg tgcgtccgca aatcgttgcg aacgcggtag aggagagttt ctgatgcagg
     7981 aactccatac gccagttcag gcagcgcagt ggctcaagtc ccgcgcatgc ggtgccttgt
     8041 gggctgacag ccgcaaagtg cgcccgggcg acgggtttgt ggcctggccc ggtgctgcag
     8101 tggatgccag aggtttcgct gctacagcca ttgcacaagg cgccggagcc tgcctgatgg
     8161 agcgcgacgg tgcagaagcc tttggactgt cgggtgagtc ttacgctacg tatgcaggat
     8221 tgaaagcgga caccgggctt attgctgccg agttttatgg acagccgtcc cgcgcactgg
     8281 atgtggttgc cgtcaccggc accaacggca agacgtccac agcgtggtgg cttgcgcagg
     8341 cactgaaaaa tttgcctgca gaacgctcct ggccgtgtgg cgtgattgga accctgggcg
     8401 ttggcattcc accggatgtg ttgtctaccg gattgaccac tcctgacccg gtcctgttgc
     8461 agcagaccct gcgggcgttt gtggatacgg gcctgaaggc ctgcgccatg gaagcgtcgt
     8521 ccatcggggt ggaggagcac cggttgactg ggaccgacat ccgtgttgcc gtgtttacca
     8581 acttcaccca ggaccatctg gattaccacg gcagcatggc gtcgtactgg gacgccaagc
     8641 ggcgtctgtt tgtgtggccg gggctgcagg cagcggtcat caatgtggat gacacacagg
     8701 gcgcccagct tgctgcagat cttgctaacg gccctctgga tgtctggact acgtcatgca
     8761 gccaggcggc acgcctgcgg gcggacaaca tccagtacgc aacccgtggt ttgcggttcg
     8821 atgtggtgga aggcgggcag accatcgcct ttcacagtca cctgattgga acgtacaacg
     8881 tggccaatat gctgggtgtg attgcagcca tgtgcagctt gggcgttccg ttggcagatg
     8941 cagtgcaggc ctgcagtggc ctgatgccgg tgccaggccg catggagtgc gttggcaacg
     9001 ctggcgagcc actggtcgcg gtggactatg cgcacacgcc ggatgcgttg gagcaggctc
     9061 tggtggcttt gcgcccgctg gcccaacaac gcgagggcgc tttgtggtgc atctttggtt
     9121 gtggtggaga ccgtgatgcc tccaagcggc ccctcatggg tgccattgct gccaagtgcg
     9181 ccgaccgggt ggtcgtcacc agtgacaacc cccgcagtga aaagccggaa gccatcattg
     9241 cccaaattct gttggggttg ccccaaactg acatggtcag tgtgcaggcg gaccgtgcgc
     9301 aggccatcgc ccgcacgctg gctgcggtcg ccgaccagga tgtcgtgctt ctggctggca
     9361 agggccatga agcgacacaa gaagtcgctg gcagcaagct gccattttcc gacagggccc
     9421 atgccgatca ggcgctgcaa gcccgccact ccgggggagt acgcccatga gtgccatgat
     9481 gaccttgcac caggccaccc agtggatgcc gggtgcccgt ttggtgggtg agaacaaggt
     9541 ggtggtgctg cgtgtccaca cggatacccg caccgttgag ccgggagatc tgtttgtggc
     9601 gctccaaggt gagcgctacg acgccaatga atttctgcaa gaggcacaac agcgcggggc
     9661 cgtgggagtg gtgtgtcacg cgggcctgcg cgcagatctg taccctgccg ggctgcccag
     9721 aatcgaggta gccgacacca aggcggcgct gcagtctctg gcaacgacct ggcgtgcgcg
     9781 atttacattg ccggtgattg cggtgactgg cagcaatggc aagaccaccg taacccagat
     9841 gcttgcagcg attctcgatg cccagtgtcc cggcggcggt gcattggcca cccgtggcaa
     9901 cttcaacaac gacatcggtg ttccgctgac gttgctgcgc ctgcgtgctg gccaccgcat
     9961 ggcggtggtc gagctgggaa tgaaccaccc gggggaaatt gccacactgg ccgcgatggc
    10021 gcagcccaca gtcgccctgg tgaataacgc ccagcgcgag cacctggagt ttatgcagac
    10081 cgtgcaggcc gtggctcggg agaacgggtc ggtgattgca tccctgccgg cggatggcgt
    10141 cgccgtggtt cccgcagacg atgaattcac ggcgctgtgg caggacctgg cggctccccg
    10201 tcggtgtgtt cgttttgccc tgtcgggtgg cgattcgcag gatgaaatgc actgcgtgga
    10261 aacccattgg gaacacggcg gctggcaggt gcgggcgcag ggcatgggcg cgcatctgca
    10321 ttaccgtctc tccattgccg gtcggcataa cgtcaagaac tccctggcgg ctgcagcctg
    10381 cgcgctggcg gctggcgtca gcgcttctgc ggtggagcag gggctgcagg cattccagcc
    10441 ggtcaaggga cgctcgcgtg cattggagat tgcagtacaa gggcgcgaac gcaccctggt
    10501 ggatgacacc tacaacgcca atccggactc ggtgcgcgcg gcgattgatg tgctggcaga
    10561 tttgccgggc ccccgcattc tggtgctggg agatatgggc gaggtgggcc acgaggggcc
    10621 ggccttccat gccgaagctg gacgctatgc gctggccaag gggattgacc agttgctggc
    10681 ccatggcaat ctggtgcgct ccgcagtaga tgtttttccc ggagcacgcc atttcgagtc
    10741 catggacgaa ctcaatgatg cagtgcttgc cggcctgccc catgccagca gtgtgctggt
    10801 gaagggctca cgcttcatgc gcatggagcg cgtggtggag gctgttgtgg cgcatttttc
    10861 gaacggagaa ccctgatgtt gctgagcctg gcccaatggc tgcaaaccct gtcgcctgaa
    10921 ttgggatttt tccgtgtgtt ccagtacctc accttccgtg cggtgatggc tgccatgacg
    10981 gcgttgctga tcggactggc tgctgggcct gcggtcattc gtcgcctgcg cgaactcaag
    11041 atcggccagc ccattcgcgg ttatggcatg gaatcccatt tgagcaagag cggcaccccc
    11101 accatgggcg gcgtgctcat tctgttgtcc atcgctatct cgacgctgtt gtggttcgac
    11161 ctcagcaacc ggtttgtctg gatcgtgttg ctggtgacct taggttttgg tgcgattggc
    11221 tgggccgacg actggcgcaa ggtggtgcac aaagaccccg agggcatgcg ctctcgcgaa
    11281 aagtatttct ggcagtccgt tgtcgggctg ctggccgcgc tctacctagt attcagcatt
    11341 tccgagagct cgaatctgcg ggtgcttgag ctgttcttca attgggtgcg ctccggcttt
    11401 gatgtcaacc tgccacccaa ggccggcctg caactgccgt tcttcaagga agtcagttac
    11461 ccgctgggtg tgttgggctt tgtggtgctg acgtacctgg tgattgtggg ttcgagcaat
    11521 gcggtgaacc tgactgatgg actagatggt ctggccatca tgccggtggt gatggtgggt
    11581 tccgccctgg gcgtgtttgc ctatgtcact ggtagcgtgg tgtattccaa gtacctgttt
    11641 tttccctaca ttccaggctc tggtgagttg ttgattttct gctctgccat ggcgggggcg
    11701 gggctcgcat tcctttggtt caacacgcac ccggcgcagg tgttcatggg ggatgtgggc
    11761 gcactcgctc tgggcggtgc tctgggcact atcgccgtga tcgtacgcca ggaaatcgtg
    11821 ctggccatca tgggcggcat ctttgtggtg gaggcgctgt ctgtaatgct gcaggtgtcc
    11881 tacttcaagt acaccaagaa gaaatttggc gagggccgcc gcctgctgaa gatggcgccg
    11941 ctgcaccacc actttgaaaa gtctggctgg aaggaaaccc aggtggtagt ccgattctgg
    12001 atcatcacca tgctgttgtg cctggtcggt ctgtccaccc tgaaactgcg atgaacgaac
    12061 acgatccgca catctccagc gacattcccc aggcatcccc cgaggacgcc ggggtagagc
    12121 tgcgcgtgcc ggatggggat atgcaagcgc cagaagccgc tcctgtcgaa gccactgtcg
    12181 ggctgttggc gggt
//
LOCUS       DAOSJM010000020        11733 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5463_length_11733_cov_4.729063, whole genome shotgun sequence.
ACCESSION   DAOSJM010000020 DAOSJM010000000
VERSION     DAOSJM010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5463_length_11733_cov_4.729063"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PKX82_01490"
     CDS             <1..653
                     /locus_tag="PKX82_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOF50947.1"
                     /translation="TFRDIATMDLDDGIAFDPESVKADAIREDSTYGGTRITLVARIG
                     SARCALQIDVGFGDAVTPEPQTVAYPTLLRNFQAPTLRVYPVYTVIAEKYQAMVMLGQ
                     ANSRMKDFYDLAVIAQRTQLDGATLAAAIAATFARRKTPLPHERPLALTSQFSEDSAK
                     LRQWQAFLNKNRIAAASLSATVALLDLLLWPPTQVAATNSPATAMWHPESGWRRNA"
     gene            1053..2228
                     /locus_tag="PKX82_01495"
     CDS             1053..2228
                     /locus_tag="PKX82_01495"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOF50948.1"
                     /translation="MKPNLSTMLSVVALTVCCTSFAQSSNPYADGNAAAPDEWLLVKK
                     GNPWWYETKPSPTPVQLQKRTPTAAEQVIIDRARALVANRPAKAFVLMDGDTVLYSET
                     KVPADADSVFFGFSMGKTVTAMAVGQAICAGKLKLETKASDIMPELNGKALGNATVRD
                     LLRMASGAADPNPDSSVWTPEQFKEWGRGNLNLVNLVTEDRIAKAAKGVFSDYKPGEV
                     STYKSTDPYVLGIMVSRATDTVWSQWIQEQILNPMGAAKPGLYVQDRQQNGLADSGLR
                     MRLEDWMRFALWVKRSSKEQGCFGDYVRAATSTQISNGSNPSTRKMGKLFGGYGYFTW
                     TENSIVPNTAWASGWGGQRIGWNTDPNNHRMVITFSNVESWMPDVYELAKDWSKLSR"
     gene            complement(2800..3804)
                     /gene="hrcA"
                     /locus_tag="PKX82_01500"
     CDS             complement(2800..3804)
                     /gene="hrcA"
                     /locus_tag="PKX82_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOF50949.1"
                     /translation="MLDDRSKLLLKALVERYIAEGQPIGSRTLSRASGLELSPATIRN
                     VMADLEELGLIVSPHTSAGRIPTARGYRMFVDTMLTVQPGQYSAASLAPDQPQKVISN
                     AANLLSNLSQFVGVVIAPRRTSSFRHIEFLRLSEKRLLVIIVSPDGDVQNRVIFTEVD
                     YTQAQLIEAANFLNSNYVGLEIEQVRERLQHEVESLRGEIAALMQAAVNVSSEAITET
                     QDEVVISGERNLLSVSDFSSDMGHLRRAFELFEQKAQLMRLMDIAGQAEGVRIFIGGE
                     SQVVPFEDLSIVSSPYEVDGKVVGTLGVIGPTRMAYDRMIQIVDITSKLVSNALSHNK
                     "
     gene            3846..4742
                     /locus_tag="PKX82_01505"
     CDS             3846..4742
                     /locus_tag="PKX82_01505"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOF50950.1"
                     /translation="MKSNFRHVALIGKYHATSSGVAGASTRKYLDEIAHFLMDQGCEV
                     VLEVDTATNIGLTDYTTMDVAGIGAHCDLCLVVGGDGTMLGIGRQLAKYKVPLIGINA
                     GRLGFITDIPFDQYKTTLGPMLAGQYEVDDRSLMQAHVMRDGHCVFSAQAMNDVVVNR
                     GATSGMVELRVEVDGHFVANQRADGLIISSPTGSTAYSLSAGGPLLHPSIPGWVMVPI
                     APHTLSNRPIVLANAAEIAIEIVAGRDASANFDMQSLASLMHGDRIVVTRSQHQVRFL
                     HPKGWSYFDTLREKMHWNEGVA"
     gene            4745..6394
                     /gene="recN"
                     /locus_tag="PKX82_01510"
     CDS             4745..6394
                     /gene="recN"
                     /locus_tag="PKX82_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOF50951.1"
                     /translation="MALKRIVLRDFVIVSELELDLQSGFTVLTGETGAGKSILIDALQ
                     LVTGARADTGVIREGAARTEVSAEFDAAPGLRPLLDEAGFEAGDTLLLRRTVDTQGKS
                     RAWVNGSPATAQQLRTIGEQLLDIHGQHAWQSLTRPDAVRGLLDAYAQVDTRTLTALW
                     SQWRVAQTALATAMQAQDSLQRERERLAWQIGELDKLAPQDDEWEELNTSHSRLAHGQ
                     ALLDAALAAVALLEEDEANALRPLNAALAQLQAQMHLEPDFQNTAEVLGSAVAQIEDA
                     VHTLRTYARRTELDPERLAELDERMTLWMGLARRYRRPPEELPALLASWKTELAQLDA
                     AADLEGLQSQEKKAQAAYMTEAKTLTKARTKAAPLLAQAITLAMQGLGMQGGKFQVTL
                     EPSAQPQQSGLEDVAFLVAGHAGSTPRPVGKVASGGELSRMALAIAVTTSQLGTAQTL
                     IFDEVDSGVGGAVAETVGRLMKQLGTDRQVLAVTHLPQVAACADHHLVVAKQLQGQAT
                     LSTVGAVQGEGRVAEIARMLGGEKLSSTTLAHAKEMLQAGT"
     gene            6394..7263
                     /gene="rapZ"
                     /locus_tag="PKX82_01515"
     CDS             6394..7263
                     /gene="rapZ"
                     /locus_tag="PKX82_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOF50952.1"
                     /translation="MEIVLITGMSGSGKSVALNALEDAGFYCVDNLPPELLLPFVELE
                     KKHQATRLAIAMDVRSATSLPLVPQQLDTLREQGIIVKPLFLDATTDTLVRRYSETRR
                     KHPLSQGESGHAERDENRALVDAIELERELLAALREQAHVIDSSIIRPSQLQGYVKTL
                     VSSPGGQLTLVFESFAFKRGVPTDADYMFDVRMLPNPHYEPGLKHLTGRDQPVIDYLE
                     QQPEVDLMLQHITHFLDNWLDALARDHRSYVTVAIGCTGGQHRSVYLVERLAQRFSAD
                     WTTLKRHREMDAL"
     gene            complement(7273..8325)
                     /gene="mutY"
                     /locus_tag="PKX82_01520"
     CDS             complement(7273..8325)
                     /gene="mutY"
                     /locus_tag="PKX82_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOF50953.1"
                     /translation="MSGTSPQGDFAAQVVQWQKVHGRNSLPWQNTQDPYRVWLSEIML
                     QQTQVVTVKAYFARFIERFPDVQSLAAATLDDVLGLWSGLGYYSRARNLHRCAQQVVA
                     EHGGAFPRDALTLATLSGIGRSTAAAIASLCFGERVAILDANVKRVVTRVLGFDADLA
                     LAANERALWDAATGLLPRGKHALVQMPGYTQGMMDLGATVCLPRKPDCAQCPVQARCA
                     AFAAGDPTRYPVRTRKLKRSSQSLWLLWVQREDGAVWLAKRPTPGVWAGLQCFPLFDD
                     EAALLSALPQRWRSAVQLLPTVTHVLTHKDLFLHPARVDITQEAINLGAGDWFEPARW
                     AAAGLPAPIRKLLENG"
     gene            complement(8322..10277)
                     /locus_tag="PKX82_01525"
     CDS             complement(8322..10277)
                     /locus_tag="PKX82_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOF50954.1"
                     /translation="MVTSFNDQFDQHGVWRREFALRLKLLAEWLKDHDLLDAAVEERL
                     RRLETQVRSDKVMVAFVAEFSRGKSEMINAIFFAGYGRRIMPASAGRTTMCPTEMGYD
                     SDVPPCLRLLPIETRLQPQALMEWRMAPEKWTRIDLDVNNPQQLALALEKVAETRLVT
                     QDDARALGFWNSQSPEDNPLVDAQGMVEVPKWRHALINIAHPLLKQGLVILDTPGLNA
                     IGAEPELTVSLIPQAQAVVFILGADTGVTKSDLSIWREHLVNEAGGDDNRLVVLNKID
                     TLWDELSSPGQIKAQIERQKRSTAEILGVSPERVIAVSAQKGLVAKVTSDAALLEQSC
                     LRQLEDVLGQGVLGRRQKILGAAMAQGISDLRAETGRVINIRKRDLTEQTMELKGLQG
                     KNASVIRHMRTRISQEQAEFDQGGAKIHAVRSVHLKLLREVFAILGPKVLKAEMAQLT
                     DSLMQKGLKLGVKRSYGETFDRLRANLNRVQGLTGEIQNMLAATFRQLNAEYGFSLQA
                     PAEPQMEAYLKDLDMVERSHLQYLGIGNAFKLAQPEFADRLVRALSTRLRAVHETALG
                     EVELWSKSAAAQLDAQLRERRRNFSRRIEAIDRIQQAAGGLEERIREIDVQEATLDQL
                     DIKLVELTDYLISSNDTHALSTELEFA"
     gene            complement(10328..11143)
                     /gene="mutM"
                     /locus_tag="PKX82_01530"
     CDS             complement(10328..11143)
                     /gene="mutM"
                     /locus_tag="PKX82_01530"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOF50955.1"
                     /translation="MPELPEVEVTRLSFADRIAGARIESLRMGKPLRWPLGVEPERLV
                     GQQVLGVRRRGKYLLIDLSDGLLLLHLGMSGSLSFGQNPGPPGVHDHCDLRTSQGVLR
                     LNDPRRFGAVVFADGENAPVAQKLLGRLGVEPLGVAFDLQQFVTALKGRKAPIKQVLL
                     AGDVVVGVGNIYASEALFLAGIRPTTRACRISRVRVARLRDAIRGVLARAVEKGGSTL
                     RDFSNAHGESGYFQLEAMVYGREGAPCRVCGTPIKAIRQGQRSTFFCQVCQKS"
     gene            11194..>11733
                     /locus_tag="PKX82_01535"
     CDS             11194..>11733
                     /locus_tag="PKX82_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50956.1"
                     /translation="MSRFKRLATLALLFPLVCSSAGAEEQATPVSNSVLGSELFYQLL
                     VGEISAQRGDASSAYALMLDAARKANSEQLYERAVALALGARSGESALTAAQAWSQAF
                     PASQQANRYVLQILIGLNRISDTVDPLRKELANTQGPARIATISLLPRYLARATDRAQ
                     AASAIERALTPDLSNKTTGA"
BASE COUNT         2391 a         3613 c         3475 g         2254 t
ORIGIN      
        1 ccaccttccg cgacatcgcg accatggact tggatgacgg catcgcgttc gaccctgagt
       61 cagtcaaagc tgatgccatc cgcgaagaca gcacctacgg cggcacccgg atcacgctgg
      121 tggcacgcat agggtccgca cgctgcgcct tgcagatcga tgtgggcttt ggtgatgcgg
      181 tgacgccaga gccgcagacc gttgcttatc ccaccttgct aagaaatttc caggccccaa
      241 cgttgcgggt ctatccggtc tacaccgtga ttgccgaaaa ataccaggct atggtgatgc
      301 tgggccaagc caacagccgc atgaaagact tctacgatct ggcagtgatc gcacagcgca
      361 cccaactgga cggtgccacc ctggccgcag cgattgccgc cacctttgcc agacggaaga
      421 ctccgctgcc acacgagcgc ccgcttgcgc tcaccagcca gtttagtgaa gactctgcca
      481 agctacgcca atggcaggcc ttcctgaaca agaaccgcat cgccgctgcc agtttgagcg
      541 ccacggttgc actgctagac ctgctgctgt ggccacctac tcaagttgcc gccaccaaca
      601 gcccggcgac agcaatgtgg cacccggaat cgggatggcg aaggaatgcc taaaacagag
      661 gatatacatt caaatatggc accaacccct gtcaaataag ataagtatgc tatcttttgt
      721 atagcattct ttgataatga attggtaggc tgttagtgac ttgaacactc gaccaaagga
      781 ttgtgggtcg aaatgatcca tttttcacga gtgtcatcac aagtcactct tgcccctttt
      841 tctcttctga atcaatagtg caagcgtcac cggatttgac aatggactgg tcgacctgtt
      901 agcaccctgg ctgcctttcg gagctgaagt tttcatcccc caaggttgga gcagtatgct
      961 tttcagggaa acgtcttaaa tgacaccgta catttgcaaa tgtgtcgtcc gcagcacatt
     1021 atttttataa cgcaatcaag gagaacccat caatgaaacc aaacctatca accatgctga
     1081 gtgttgtcgc acttacggtt tgctgtactt cttttgccca atccagcaac ccctatgctg
     1141 atggcaatgc cgccgcgcct gacgaatggt tgttggtcaa aaaaggcaat ccgtggtggt
     1201 atgaaaccaa gccaagtccc acgccagttc aacttcaaaa gcgcacgccc acggcagcgg
     1261 agcaagtcat cattgatcga gcacgcgcat tggtggcaaa tcgacccgcc aaagcgtttg
     1321 ttttgatgga cggtgacacg gtgctgtact cggagacaaa agtgcctgca gacgctgatt
     1381 ccgtattctt tggcttctcc atgggcaaga cggtcacagc tatggccgtt ggtcaggcca
     1441 tatgcgcggg aaagctcaag ctggaaacca aggccagtga catcatgccg gaactcaatg
     1501 gcaaggcatt gggcaacgcc accgtgcgcg atctgctgcg catggcatcc ggtgcggctg
     1561 atcccaaccc agacagttca gtctggacac ctgagcagtt caaagagtgg ggacgtggca
     1621 atctgaatct ggttaacttg gtgacggagg atcgtattgc caaagccgcc aagggcgtgt
     1681 tctcagatta caaaccgggt gaagtgtcca catataagag tacagacccc tacgtgctgg
     1741 gcatcatggt cagccgcgcc acggatacgg tgtggagcca gtggattcag gagcaaattt
     1801 tgaacccaat gggtgcggcc aaaccgggtc tgtacgttca agaccgtcaa caaaatggac
     1861 ttgccgacag cggtctacgg atgcgtttag aggactggat gcggttcgcc ttgtgggtca
     1921 agcgcagttc aaaagagcag ggttgttttg gtgactacgt gcgtgcagcc accagcactc
     1981 agatttccaa cggaagtaac cccagtaccc gcaagatggg caagctgttt ggtggctatg
     2041 gctacttcac ttggacagag aacagcatcg tacccaatac ggcatgggct tctggctggg
     2101 gtggtcagcg catcggctgg aatacagacc cgaacaacca tcggatggtc atcactttct
     2161 ccaacgtgga aagctggatg ccggacgttt acgagcttgc caaagactgg agcaagcttt
     2221 cgcgataggc tttgtgacaa acagacaaag aaaaatgcca ccgtgaaggt ggcatttttc
     2281 cagttttggt aggccgtgac agacttgaac tgtcgaccaa aggattatgc gtaccacttc
     2341 gactttcgcc gcccgtttcc ggtttgtggt ctggactcta tcttgccttt cggccttccc
     2401 gtcgagtctc tacacgttct cctttcggag cttcgctcgg tattggcatg gcgcttttga
     2461 cacctaagct ttcaccgaat ttgagaagtt ctacatccag gcagagttaa cttacccagt
     2521 gcaacccatt ttgaaaaaac catgcgctta cgcgtttagg cttcttcacg ttaagtcctc
     2581 tgctctaacc aactgagcta acggcccaaa acttttacca gcactgcgca tcggaaatca
     2641 ctgcaattcc ttgccgatca cccactgctg gttgcaatac ggttgcaatg tccacctcag
     2701 tggagttttt gcaactgcaa caaattcaaa ggagtcatct gctctaacca actgagctaa
     2761 ctccccaacc caaccggtga ttgtaccggg cgtgcagggc tatttgttgt ggctcagtgc
     2821 gttactcacc agcttggagg tgatatccac gatctggatc atgcggtcgt aggccatgcg
     2881 ggtcgggccg atgacaccca gggtgccgac caccttgccg tccacctcgt aagggctgct
     2941 gacgatggac aggtcctcaa acggcaccac ctggctctcg cctccgataa agatgcgcac
     3001 accttcggcc tgcccggcga tgtccatcag acgcatgagc tgggcctttt gctcaaacag
     3061 ttcgaacgcg cggcgcaggt ggcccatgtc actggaaaag tccgacaccg acagcagatt
     3121 gcgttcgccc gaaatcacca cctcgtcctg cgtctcggta atggcctcgg agctgacgtt
     3181 gaccgcggcc tgcatcagcg cagcaatctc gccgcgcagc gattccactt cgtgctgcag
     3241 gcgctcgcgc acctgctcga tctccagccc cacgtagttg ctgttgagga agttggcggc
     3301 ctcgatcaat tgggcctggg tgtagtccac ctcggtgaag atgacgcggt tttgcacatc
     3361 gccgtctggc gacacgatga tgaccagcag gcgcttctcg gacaggcgca gaaactcgat
     3421 gtgcctgaac gacgacgtgc ggcgcggcgc aatgaccacg cccacaaact gcgacaggtt
     3481 ggacagcagg ttggccgcgt tggaaataac cttctgcggc tggtcaggcg cgaggctggc
     3541 cgcggaatac tgtccgggct gcaccgtgag catggtgtcc acaaacatgc ggtagccgcg
     3601 cgcggtgggg atgcgcccgg ccgaggtgtg ggggctgacg atcaggccca gttcttccag
     3661 gtcggccatc acgttgcgga tggtcgcggg cgacagctcc agacccgacg ccctggacag
     3721 cgtgcggctg ccgatgggct gcccttcggc gatgtagcgc tctaccaggg ctttcaacaa
     3781 caacttggaa cgatcatcga gcatccacgc attctagtga gctaatttag tgttgtaatt
     3841 tgcgcatgaa atccaatttc cggcacgtgg ccctcatcgg caaataccat gcaacgagct
     3901 cgggtgttgc cggtgcttcc acgcgcaagt acctcgacga gattgcgcat ttcctgatgg
     3961 accagggctg cgaggtggtg ctggaagtgg acaccgccac caacatcggc ctgaccgact
     4021 acacgaccat ggatgtagcc ggcatcggcg cccactgcga cctgtgtctg gtggtggggg
     4081 gcgacggcac catgctgggc ataggacggc agcttgccaa gtacaaggtg ccgctgatcg
     4141 gcatcaacgc tgggcgcctg ggctttatca ccgacattcc gtttgaccag tacaagacca
     4201 cgctgggtcc catgcttgca ggccagtacg aggtggacga ccgcagtctg atgcaggccc
     4261 atgtgatgcg cgacgggcac tgcgttttct cggcacaggc catgaacgat gtggtggtga
     4321 accgcggcgc cacgtcgggc atggtggagt tgcgtgtgga agtggatggg cactttgtgg
     4381 ccaatcagcg cgccgacggt ctgattattt ccagccccac ggggtcgacg gcctattcgc
     4441 tgtcggcggg tgggccgctg ctgcacccgt ccattccggg ctgggtgatg gtgcccatcg
     4501 ccccgcatac attgtcaaat cggccgattg tcctcgccaa tgctgcggag atagctatcg
     4561 aaatagtagc aggccgagat gccagcgcca actttgatat gcagtccctg gcctcgctga
     4621 tgcacggcga ccgcattgtg gtgacgcgct cgcagcacca ggtgcggttt ctgcacccca
     4681 agggttggtc gtatttcgac acgctgcggg aaaagatgca ctggaatgaa ggagtggcct
     4741 aaccgtggcg ttgaagcgaa tcgtcctgcg cgactttgtc attgtcagcg aactggagct
     4801 ggacctgcag tcgggcttca ccgtgctgac cggtgaaacc ggcgcaggca aatccatcct
     4861 gatcgatgca ttgcagctgg tcaccggcgc gcgcgccgac accggcgtga tccgtgaagg
     4921 cgccgcccgc accgaagtga gtgccgagtt tgacgccgcg ccaggcctgc ggccgctgct
     4981 cgatgaagcc gggtttgagg ccggcgacac cttgctgcta cgccgcacgg ttgataccca
     5041 gggcaagagc cgcgcgtggg tcaatggcag tccggccacg gcccagcaac tgcgcaccat
     5101 tggcgagcaa ctgctcgaca tccatggcca gcatgcctgg caaagcctga cgcgaccaga
     5161 cgcggtgcgc ggcctgctcg atgcttacgc ccaggtggac acccgcaccc tcaccgcact
     5221 gtggagccaa tggcgtgtgg cccagacggc gctcgccacc gccatgcagg cgcaggactc
     5281 actgcaacgc gagcgcgaac gcctggcctg gcagattggc gaactcgaca agctcgcgcc
     5341 ccaggacgac gaatgggaag aactcaacac cagccacagc cgcctggccc atggccaggc
     5401 actgctggac gccgcgctgg ctgcggtggc cttgctcgaa gaagacgagg ccaacgccct
     5461 gcgtccgctc aatgctgcgc tggcacagtt gcaggcgcag atgcatctgg agcccgactt
     5521 ccagaacacg gcggaggttc tgggctctgc cgttgcacag attgaagacg cagtacacac
     5581 cctgcgcacc tacgcacggc gcaccgagct ggacccggag cgcctggccg aactggacga
     5641 gcgcatgacg ctgtggatgg gcttggcacg ccgctaccgc cgcccgccgg aagaactgcc
     5701 ggcgctgctg gcatcctgga aaaccgagct ggcccagctg gatgcagcag ccgacctgga
     5761 aggcctgcaa tcccaggaga aaaaggcgca agccgcttac atgaccgagg ccaaaaccct
     5821 caccaaggcg cgcaccaagg ccgcccccct gctggcgcag gccatcactc tggccatgca
     5881 aggcctgggc atgcagggcg gcaagttcca agtgacgctg gagcccagcg cgcagccgca
     5941 gcaaagcggc ctggaagacg tggcattttt ggtagccggg cacgcgggca gtaccccgcg
     6001 gccagtgggc aaggtggcgt cgggtggcga gctgtcccgc atggcactgg ccattgcggt
     6061 caccaccagc cagctgggta ccgcgcagac actgatattt gacgaggtgg actcgggcgt
     6121 gggcggcgca gtggccgaaa ccgtgggccg cctgatgaag cagctgggca ctgaccgcca
     6181 ggtgctggcc gtgacccacc tgccacaagt agctgcctgc gcagaccacc atctggtggt
     6241 ggccaagcag ctgcagggcc aagccacgct cagtacagtg ggcgcagtgc agggcgaagg
     6301 acgcgtggcc gaaatcgcgc gcatgctggg cggcgaaaag ctttccagta ccaccctggc
     6361 ccacgccaag gaaatgttgc aggcgggtac gtaatggaaa ttgtgctgat caccggtatg
     6421 tcgggttccg gaaaatccgt ggcgctcaat gcgctggaag atgccggctt ctactgcgtg
     6481 gacaacctgc cacccgagct gctgttgcct tttgtggagc tggaaaagaa acaccaggca
     6541 acccggctgg ccattgcgat ggatgtgcgc agcgcgacct ccctgcccct ggtgccccaa
     6601 cagctggaca ccctgcgcga gcagggcatc atcgtcaagc cgctgtttct ggatgccacc
     6661 accgatacgc tggtgcgccg gtattcggaa acccggcgca agcatccgct gtcccaaggt
     6721 gaatcgggcc atgcagagcg cgacgaaaac cgtgcgctgg tagatgccat cgagctggag
     6781 cgcgaactac tggccgccct gcgggagcag gcccatgtga ttgactccag catcatccgc
     6841 ccgtcgcagt tgcagggcta tgtcaaaacc ctggtgtcct cacccggcgg gcagttgacg
     6901 ctggtgtttg agtctttcgc cttcaagcgc ggcgtgccga ccgacgccga ctacatgttt
     6961 gacgtgcgca tgctgcccaa cccgcactat gagccaggcc tcaagcacct gacgggacgc
     7021 gaccagccgg taattgacta tctggaacag caacccgaag tggacctgat gctgcagcac
     7081 atcacccact ttctggacaa ctggctggat gccctggcgc gcgaccaccg cagctatgtg
     7141 acagtggcca ttggttgcac cggcggacag catcgctcgg tgtacctggt ggaacggttg
     7201 gcccagcgct tcagtgcgga ctggaccacg ctcaagcgcc accgcgagat ggacgcgctg
     7261 tagcctcgtc gctcagccgt tttcgagcag cttgcggatc ggtgcgggca agccggccgc
     7321 cgcccagcgg gcaggttcga accagtcacc cgctcctaaa ttgatagctt cctgcgtaat
     7381 atctacgcgg gctggatgca gaaaaaggtc tttatgtgtc agcacatggg tgaccgtggg
     7441 tagcagttgc acggcacttc tccaacgctg cggcagcgcg gacagcaatg ccgcttcatc
     7501 gtcaaacagc gggaagcact gcagccccgc ccacacgccc ggcgtgggtc gcttcgccag
     7561 ccacacagcg ccatcctcac gttgcaccca cagtagccac agcgactggc tgctgcgctt
     7621 gagtttgcgg gtgcgtaccg ggtaacgggt cgggtcacct gcggcgaaag cagcacagcg
     7681 cgcttgcacc gggcactggg cacagtcggg ttttctgggc aagcacaccg ttgcgcccag
     7741 atccatcatg ccctgggtgt aacccggcat ctgcaccagc gcatgtttgc cacggggcaa
     7801 caaccctgtc gccgcatccc acagcgcacg ctcattcgcg gccagagcca gatcggcatc
     7861 aaaacccagc acacgcgtca ccacgcgttt gacgttggca tccagaatgg ccacccgctc
     7921 gccgaaacac agggacgcaa tggcggcggc agtggagcgg ccaatgcccg atagcgtggc
     7981 cagtgtcagt gcatcgcggg gaaatgcgcc gccgtgctca gccacgacct gctgggcgca
     8041 gcggtgcagg ttgcgcgccc ggctgtagta acccaggcca ctccagaggc ccagtacatc
     8101 gtccagtgtg gcggctgcca aagattgcac gtcgggaaag cgttcaatga agcgcgcaaa
     8161 gtaagctttg actgtcacca cctgcgtttg ctgcaacatg atttcggaca gccagacccg
     8221 gtagggatcc tgcgtgttct gccagggcag gctattgcgt ccgtggacct tttgccactg
     8281 gacgacctgc gctgcgaaat cgccttgcgg cgaagtaccg ctcaggcgaa ctccagttcc
     8341 gtggacagtg cgtgcgtgtc gttcgacgag atcaggtagt cggtcagctc caccagcttg
     8401 atgtcgagct ggtcgagtgt ggcttcctgc acatcgattt cgcggatgcg ctcttccagc
     8461 ccgcctgcgg cctgctggat gcggtcaatc gcctcgatgc gacgcgagaa attgcggcgg
     8521 cgctcgcgca actgtgcgtc aagctgtgct gcagcggatt tgctccagag ctccacttcg
     8581 cccagcgccg tctcgtgtac tgcacgcaaa cgtgtggaca gtgcacgcac cagccggtcg
     8641 gcaaactccg gttgcgccag tttgaatgca ttgccaatgc ccaggtactg gaggtggctg
     8701 cgctcaacca tgtccagatc cttcaagtag gcttccatct ggggttcggc aggcgcctgc
     8761 agcgaaaaac catactccgc attgagctgg cggaaggtgg cggccagcat gttctggatt
     8821 tcgcccgtca ggccctgcac ccggttcagg ttggcgcgca ggcggtcaaa ggtttcgcca
     8881 tacgagcgtt tgacacccaa cttgagaccc ttttgcatca gcgaatcggt cagctgggcc
     8941 atttccgcct tgagcacttt cgggcccaga atggcaaaca cttcgcgcag caacttcagg
     9001 tgcacagaac gcaccgcatg gatcttcgcg ccaccctggt caaactcggc ctgctcctgc
     9061 gaaatgcgcg tacgcatgtg gcggatcaca gaggcatttt ttccctgcaa ccctttgagc
     9121 tccatggtct gttcggtgag gtcacgcttg cgaatgttga tgacccgccc ggtttccgca
     9181 cgcaaatcgg agatgccctg tgccatggcc gcgccgagaa ttttctgccg gcggcccagc
     9241 accccctgac ccagcacgtc ttcgagttgg cgcaggcagc tttgctccag caaggctgcg
     9301 tcactggtga ccttggcgac caggcccttc tgggcagaaa ccgcaatcac ccgctcgggc
     9361 gagacgccca gaatttccgc ggtgctgcgc ttctggcgct cgatctgcgc cttgatctgc
     9421 ccgggggaac tcagctcgtc ccacagagta tcgatcttgt tgagcaccac caggcggttg
     9481 tcatcgccac ccgcctcgtt aaccagatgc tcgcgccaga tggacaagtc ggacttggtg
     9541 acgccggtat cggcccccag gataaacacc acggcctgtg cctgcggaat caggctgacg
     9601 gtcagctcgg gctcggcacc aatggcattg aggccgggcg tatccagaat caccagcccc
     9661 tgcttgagca agggatgggc aatgttgatc agggcatggc gccacttggg cacttcgacc
     9721 atgccctgtg cgtccaccag cgggttgtct tcgggcgact ggctgttcca gaagcccagc
     9781 gcccgggcgt cgtcctgggt gaccaggcgc gtctctgcca ctttttccag tgccagtgcc
     9841 agttgctggg gattgttgac gtcgaggtcg atgcgtgtcc acttctcggg cgccatgcgc
     9901 cactccatca gtgcctgcgg ctgcaaccgg gtttcgatgg gcaacaggcg aaggcaggga
     9961 ggcacatcag agtcgtagcc catttcggtg gggcacatgg tggtgcgacc tgcgctggcg
    10021 ggcatgatgc gacgcccgta acccgcaaag aagatggcgt tgatcatctc ggacttgccg
    10081 cgggaaaatt ccgcaacaaa tgccaccatc accttgtcgg aacgtacctg ggtctccaga
    10141 cgccgcaagc gttcttccac tgcggcgtcg agcaggtcat ggtccttcaa ccattcagcc
    10201 agcaacttca ggcgcagcgc aaattcacgt cgccacacac catgctggtc aaattgatcg
    10261 ttgaatgaag taaccaagag tctcccctag aagcgatcaa atatagcact ctggtgcccg
    10321 gctgtgctca gcttttctgg cacacctggc agaaaaacgt ggatcgctgc ccctggcgga
    10381 tggccttgat gggcgtcccg caaaccctgc agggcgcccc ctcacggcca tacaccatgg
    10441 cctccagctg gaaataaccc gattcaccat gggcattgga gaagtcgcgc aacgtgctcc
    10501 cgcccttttc gactgcacgc gccaatacgc cccgaatggc gtctctcaga cgggccacgc
    10561 gcacacggct gatcctgcag gcccgtgtag tgggccggat accggcaaga aacagggcct
    10621 cggaggcgta gatattgccc acgcccacca ccacatcgcc ggccagcaac acctgcttga
    10681 tgggtgcctt gcgccccttg agtgcggtta caaactgttg caaatcaaac gctacaccca
    10741 gaggctccac gcccagcctg cccagcagct tctgggcgac cggagcattc tccccatccg
    10801 caaacaccac ggcgccgaat cggcgcgggt cgttgagtcg cagcacgccc tggctggtgc
    10861 gcaaatcgca gtggtcatgc acgcccggcg ggcccgggtt ctgaccgaag gacagactgc
    10921 ctgacatgcc caagtgcagc agtagcagcc cgtcactcaa atcgatcaag aggtatttgc
    10981 cccgtcggcg cacacccagc acttgctggc ccaccagacg ttcaggttcc acccccagcg
    11041 gccaccgcag gggcttgccc atgcgcagcg attcaatgcg tgcaccggca atgcgatcgg
    11101 caaagctcag gcgggttact tctacctcgg gcaattcggg catggcattt ccggtggcaa
    11161 acagggggag cggggttgga ttattatggt ccgatgtcac gattcaagcg tctcgccact
    11221 ctggcactcc tcttcccctt ggtctgcagc agcgctggcg ctgaagagca ggcaacgccc
    11281 gtctcgaact cggtcctggg cagtgagctg ttttaccaat tgttggtggg agagatcagc
    11341 gcgcagcgcg gcgatgcttc gtccgcctac gccctgatgc tggacgccgc acgcaaggcc
    11401 aactcggagc agctgtatga gcgcgccgta gcactggcat tgggggcgcg cagcggggaa
    11461 tcggcactga cagcagcgca ggcctggtca caggcttttc cggcctccca gcaggccaac
    11521 cggtatgtgc tgcagattct gattggactg aaccggattt ccgatacggt agacccgctc
    11581 aggaaagagc tggccaacac gcaggggcct gcacgcattg ctaccatcag cctgctgccc
    11641 cgttatctgg cacgcgccac cgacagggca caggctgcca gcgccatcga gcgggcgctt
    11701 acaccggatc tgagcaacaa gaccaccggg gct
//
LOCUS       DAOSJM010000021        11682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5490_length_11682_cov_4.241077, whole genome shotgun sequence.
ACCESSION   DAOSJM010000021 DAOSJM010000000
VERSION     DAOSJM010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5490_length_11682_cov_4.241077"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..476
                     /locus_tag="PKX82_01540"
     CDS             9..476
                     /locus_tag="PKX82_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HOF50957.1"
                     /translation="MKTLFASLLLSSLALAASAETIGTVSTTFKLLSPNDKVVVDVFD
                     DPAVNGVACYLSHAKTGGYAGALGLAEDTSDAAVACRQVGPISFKEKIDKQDEVFKAS
                     ASFLFKHVRVVRMVDVKRNTLVYLVYSDKIIDGSPKNSVTAVAVPAEQPIPIK"
     gene            complement(553..2766)
                     /locus_tag="PKX82_01545"
     CDS             complement(553..2766)
                     /locus_tag="PKX82_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOF50958.1"
                     /translation="MATAIGLIDNYYQQHFQHWIGAGEDLYFAVQHTATAYLDSKPAL
                     RGKHKVTRAERHAAFWSSSFLTDLSAQAWQSQAMMLALAQYMGQERVSNMAMLANVAS
                     VAPDVLVRAVRCSGLVLNQHSPRRTELGLLAAANPLIAELCKVLGIFDVAHKERNSAV
                     SMWQSMLTELSPLELLSYASLYAFEHWVPARFDTASLSADAQADMQAAWDAVNDILLW
                     KLANCTDDSTRLADTVIGPALAKHLSPFLFPSPGGQPARHDLREAFANLLAAQIELNS
                     FVAQSANAFSYDHSIEFVRVGDRLEINELDAAARTAWDRNGRKLSALHTYWHYRAVDA
                     FVDSGKATQQIGSAENHALNQVAYIKALRTQLQLNDVYGVSDTVSTDAGDTVPLFQAL
                     LSLELMNVFFQRDFLETFVEHLRSTGHWAKALSMLAFNGLRDGNQNRFPLTWSSRDDK
                     VAKMVGWTVTAEQPKGNPRHAAAIVDFWTSDWSKLATQLRGGAAGLKPDLFERPILKM
                     GQTLVQLPWVVGLQNNSSAAINNLRRLGARRNEARSETQRIEAQLGAMLEKRGFKVVL
                     NWSPPVGPDGNAGEVDVICACDCAVLVLEVKSTYLRRSQQDAWQHETTTLRNAGQQLH
                     RKVAAVQLALPDHPELALRLGLKPGAPAPAIYGWIVDTSIECDHQRLGGFLKVSVEEL
                     LIALRDERHLLHDPDGMFAQTAPAARAVPQSLYPQAFSVGRFIEVVETQAVWEGI"
     gene            2906..4756
                     /locus_tag="PKX82_01550"
     CDS             2906..4756
                     /locus_tag="PKX82_01550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF50959.1"
                     /translation="MVVRVFLRWVVMLCCGLLWLAPVALWAQGMSVAQPGTGAQDATP
                     HLQYLEDTTAALDFAQVQQAYAQGQFQGTAGVKEALSLGFTRSAFWLRLDVANRAPSA
                     ALQMLEVVNARISYVTLYAPGPEGRYQETATGGDMPFATRAYANRNFVFPMAVPAGTT
                     QTVYLRVQSTIGLLVPVQLWAQTDFAHHERNDYMVQALYLGVALAMAVFNLLLFVTLR
                     DRAYALYVIFVGFSVLALGQKTGLASEYLWPQSLTWVNPNYYTATSWGLVCFMAFMRS
                     MLRTPQNMPRIDRAMRAGMWLHALMPLLYWTAVQSVARATIMLYIVSCLALFVLVGWC
                     MYKRMRAAYFFGAAFAALMTGSLMTLLRALGVLPTNAFTVDGLQAGSTLEMLLLAFAL
                     ADRYNTLRKDKLQAQSDLVLAKEKLVETLQASERLLTQRVEERTQQLQVLNEKLEALT
                     LVDDLTGVANRRQFDQTLHKEWLRMERLHQPLALLMLDVDWFKPYNDHYGHQAGDTCL
                     RAVATAIAALSRTSDLVARYGGEEFVLIVPATDSESAMALARRACEAVQALALEHVRS
                     DKGVITISVGVAALVPGTGHTVEELLRAADAALYRAKKQGRNQAVLAHDL"
     assembly_gap    4779..4788
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4815..5381
                     /locus_tag="PKX82_01555"
     CDS             4815..5381
                     /locus_tag="PKX82_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HOF50960.1"
                     /translation="MRPIPAFIFASRWLQLPLYLGLIAAQGVYVFHFWVELVHLLEAA
                     FGSQTALQALVDSIGYKADAQVTHLNETIIMLVVLALIDVVMISNLLIMVIVGGYETF
                     VSRMDLEGHPDQPEWLGHVNASVLKVKLGLSIIGISSIHLLKTFINAANYSEKVLMWQ
                     TIIHVTFLLSALAIAYTDKLLTSNQSSH"
     gene            complement(5386..5976)
                     /locus_tag="PKX82_01560"
     CDS             complement(5386..5976)
                     /locus_tag="PKX82_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOF50961.1"
                     /translation="MHRTIFTTPVVNTLLRAFSVTYLKLTGWKVQGSLPPECAKSVFI
                     AAPHTSNWDLPNTLMVAFALRLNIYWMGKEQIFKPPFRGLMQWLGGISVNRQQATNMV
                     AASVEAIVAADGPVQLIVPPEGTRSKVRYWKTGFYYIALGAKVPIVMAYMDYEKKISG
                     LGPVFMPTGDIEADMVKIKAFYAPFKGKNASQFTAE"
     gene            complement(6006..6962)
                     /locus_tag="PKX82_01565"
     CDS             complement(6006..6962)
                     /locus_tag="PKX82_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOF50962.1"
                     /translation="MNFVDIDDASEHSPHTAEERNAAASRSTWVSVAVNVVLSTTQIV
                     VGVMAKSQALIADGIHSLSDLVSDFVVLFAGHHSQKDADEDHPYGHHRFETAASLALG
                     VILMAVGVGMLWSALQKLQSPDTIATVHASALWVAFAALVLKELLFRYMLRVAKAVKS
                     SMLVANAWHARSDAASSLVVAIGLIGNLMGYPLLDPIAALIVGFMVAKMGWSFGWDAL
                     HDLMDRAVDEQEVQAIRQTLVSTPGVAGVHDIRTRKMGDMIVVDAHLEVDANITVEAG
                     HNIAVAARQAVLQRHRVLNLMTHVDPAHRPDLDHVPTPPSPR"
     gene            7168..8193
                     /locus_tag="PKX82_01570"
     CDS             7168..8193
                     /locus_tag="PKX82_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF50963.1"
                     /translation="MGFIGGLSDRGSDVGEGGRNGLMLAIEQRNAAGGILGRPIELLV
                     QDDGQNSVQAMEAVRALVDANVDVVVGPFTSAMTAAVLPQMNQARVVIISPTIAGEEF
                     VGKDDYLFRINAAAVTNAGNYAQMLKLRGVHKVGLVYDLDNRKFTESWSKAFEAAFAS
                     IGGRVVAAVPFESNREPAFGALVAKALVGKPDGLVFLATPVDTARLAQQSSKMAPGLA
                     MATSEWAASENLLELGGPAVEGMLTLRVYNRDDPSERTRLFLEAYQARFSRAPGDSAV
                     AAYDAATALMEALVRKSSGESLKEALIRYGPFEGLQQPILFDANGDTNRTMYFTEIRA
                     GRYALVK"
     gene            8193..10493
                     /locus_tag="PKX82_01575"
     CDS             8193..10493
                     /locus_tag="PKX82_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF50964.1"
                     /translation="MNTLARSWRLRSVLVGLVAATILLTFTLVGGTILLVQIPNLKRE
                     ARADVAREAAEVTARVETTLGTLESRLEFLGQTLQTGATMQQSNAMLDRALGAGPAIR
                     AIYLVSLKGKVLAAGLAAEQRGLRADLLGSDVSATRLFREVVQRRRLVWGDKYLSALS
                     GAVTVGVAMPVDSDLVLMAEIPISYLLSTVQLAIGGKNSSVWLVDRAGEVLVDTMGGR
                     FVNTVNMLNLPVVQAAVAGRELPREFEMAGRSYYPGVSRSGKLDWYVVAGIPAGLENS
                     RIRATVIYVAGGFVGSLLVGLLLAPLWSARLTQALTRIVGRADQITRGEVAGQWPRSL
                     ITEFNQISTDLEKMATGLQEREQKLQAVFNTAPVAMSLTDMDNHSVLIDVNEAWCRTF
                     ARRREEALGRSGVELGLWRSLEERDAAVQRSVTTGGMVETWAMRGDGVPILCQAFRRE
                     LRLEDANLMIWASVDITAVRRTEDALRDLNADLEARVASRTRQLEKLNTELSGAFENL
                     RMTQGELVRSEKMAALGSLVAGVSHELNTPLGNGLMAVSALADAVRSFKGTMQSGLRR
                     SDLEDFVLRVDQGADIAVRNLQRAADLVVSFKQVAVDQTSSQRRRFELSEVVHEMVIS
                     LGPTLRRTPYEVVLDVPKIGLLLDSYPGPLGQTLGNLINNAVMHAFDGRQNGTIRITG
                     YRDVNGWIALRVEDNGQGIPAALLPRIFDPFVTTKMGRGGTGLGLHIAYNAVTTLLGG
                     TLTVHSVQGQGTTFELHLPEQAPLAV"
     gene            complement(10490..>11682)
                     /locus_tag="PKX82_01580"
     CDS             complement(10490..>11682)
                     /locus_tag="PKX82_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HOF50965.1"
                     /translation="LTGEWEYKLNQMEHGKMSREAFMAEIASMTERMVKKAKEYDRDT
                     IPGDYATLSSPCPNCGGIVKENYRRYTCTGKSGAEEGCGFSFGKSPAGRTFEQAEVEQ
                     FLRDKHIGPLDGFRSKAGWPFVAEMVIKFDEDTKNYKLEFDFGDDKAGEESGEIIDFS
                     GKESLGTCPKCGGHVFEHGKNYVCDRSVPTHEHPTPSCDFKSGQIILQQPIEREQMQK
                     LLATGKTDMLEKFVSMRTRRAFKAMLAWDPEAGKVNFEFAPSKFPPRKTAAKTAAKTT
                     ATKTGAARAASTGATAKKAPAKKAVASKTAAAKVAKPKAPRKTTAASGKTPSAELAAV
                     IGPELIARPQVMKKLWDYIKANGLQDAKDKRSINADAKLLAVFGKPQVTMFELAGIAG
                     KHLS"
BASE COUNT         2048 a         3482 c         3812 g         2330 t
ORIGIN      
        1 agttagcaat gaaaacacta tttgcctcgc tgctgctcag cagccttgcg ctggctgcca
       61 gcgccgagac cattggcacg gtcagcacca ccttcaaact gctcagcccc aacgacaagg
      121 tggtggtgga tgtgtttgac gacccggcgg tgaatggcgt ggcgtgttac ctgtcgcacg
      181 ccaaaaccgg cggctacgcc ggtgcgctgg gcctggcaga agacacatca gacgccgccg
      241 tggcctgccg ccaggtgggc cccatcagct ttaaagaaaa gatcgacaag caagacgagg
      301 tgttcaaggc cagcgcctcg ttcctgttta aacacgtgcg cgtggtgcgc atggtggatg
      361 tgaagcgcaa caccctggtc tatctggtgt actccgacaa gatcattgac ggcagcccca
      421 aaaacagcgt gacggcggtg gcggtgccgg ccgagcagcc gattcctatc aaataggttt
      481 tataaggaaa attaggctgt agcccacgtg aaatatgcgc acgaagctat caaatacata
      541 gcaatttgaa actcaaatac cttcccacac cgcctgcgtc tccaccactt caatgaaccg
      601 tccaacgcta aacgcttgcg ggtaaaggct ctgtggcact gcacgggcgg caggcgcagt
      661 ctgcgcaaac atgccgtccg ggtcgtgcag caaatgccgc tcatcgcgca gcgcaatcag
      721 cagctcttcc acagacacct tcagaaagcc gccaagccgc tggtggtcgc actcgatgct
      781 ggtgtctact atccatccgt agatagcggg ggctggcgca ccaggtttca gcccaaggcg
      841 cagcgccaat tcggggtgat cgggcaacgc tagttgcaca gcagccacct tacggtgtag
      901 ttgctgaccc gcattgcgca gcgtagtggt ctcgtgctgc catgcgtcct gctgggaccg
      961 gcgcaggtag gtggacttca cctccagcac cagcaccgcg caatcacacg cgcagatcac
     1021 atccacttca ccagcattgc catctggccc taccggcggg ctccagttca acaccacttt
     1081 gaaaccgcgc ttctccagca tggcccccag ctgggcttct attcgctgag tctcggagcg
     1141 cgcctcattg cgccgcgcac ccaaacgccg cagattgttg atggccgccg agctgttgtt
     1201 ttgcaggccc acaacccatg gcaactgcac cagggtctgg cccattttta ggatcgggcg
     1261 ctcaaacagg tcgggtttca agcctgcagc gccgccgcgc aattgggtgg ccagcttgct
     1321 ccagtcactg gtccagaaat cgactatcgc cgcagcatgg cgcgggttgc ctttcggttg
     1381 ctctgctgtg acagtccagc ccaccatctt ggcgaccttg tcatcacggc tggaccaggt
     1441 gagcgggaag cgattctgat tgccatcccg caagccattg aaggccagca tgctcagcgc
     1501 cttggcccag tggcccgtgc tgcgcaaatg ctccacaaat gtttccagaa aatcccgctg
     1561 gaaaaaaacg ttcatcaact ccagcgacag cagcgcctga aacagcggca cggtgtcgcc
     1621 cgcatcggtg gacacggtat cgcttacgcc atacacatcg ttcaattgca actgcgtgcg
     1681 cagcgccttg atgtaggcaa cctgattaag tgcgtggttc tcagcgctgc caatctgctg
     1741 cgttgccttg ccggaatcca caaacgcatc cacggcgcgg tagtgccagt aggtgtgcag
     1801 ggcggacagc ttgcggccat tgcgatccca cgccgtgcgg gctgccgcat ccagttcatt
     1861 gatctccaga cgatccccca cccgcacaaa ctcaatgctg tggtcgtagc tgaaggcgtt
     1921 ggccgactgg gccacaaagc tgttcaattc aatctgtgcc gccagcaggt tggcaaacgc
     1981 ctcgcgcaag tcgtgccggg caggttgacc gccgggcgat ggaaacaaga agggtgacaa
     2041 gtgtttggcg agcgccgggc caatgaccgt atcggccaat cgagtcgaat catccgtgca
     2101 gttggcaagc ttccagagca ggatgtcgtt gactgcatcc cacgccgcct gcatgtccgc
     2161 ttgcgcgtcg gccgaaaggc tggccgtatc gaatcgagca ggcacccaat gttcaaacgc
     2221 atacaggctg gcatagctca gcaactccag cggtgacaac tcagtcagca tgctttgcca
     2281 catcgagacg gcgctattgc gttccttgtg tgccacgtca aaaatgccca gcaccttgca
     2341 caactccgca atcagcgggt tggccgcagc cagaagaccc agctcagttc gtctgggcga
     2401 atgttggttg agcaccagcc cggaacaacg cacggcacgt accagcacgt cgggcgcaac
     2461 cgaggccaca ttggcgagca tggccatgtt ggacacgcgc tcttgcccca tgtactgggc
     2521 cagcgcaagc atcatcgctt gcgactgcca tgcttgcgcg gacaagtcgg tcaggaaact
     2581 gctggaccag aaagcggcgt ggcgctctgc tcgggtgact ttgtgtttgc cgcggagtgc
     2641 gggtttgctg tcaaggtatg ccgtcgcagt gtgttgcaca gcgaagtaca gatcttctcc
     2701 tgcgccaatc caatgctgaa aatgttgctg gtaatagttg tcgatcaaac caatcgcagt
     2761 cgccattgct ttgccaccgc cctgagtgaa tatttttatg caacgcatca tgccgggaat
     2821 gcccgttctt ggctaggtgc cgtacgcacg gtttcaccgg cgtgcggtaa gctctcgggc
     2881 cgatgttggc gatggagttg ggttcatggt ggtgagggtg ttcttgcgct gggtggtgat
     2941 gctgtgctgc ggcctgctgt ggcttgcgcc ggtggcactg tgggcgcagg gcatgtccgt
     3001 ggcccagccg ggcaccggcg cacaggacgc cacgccgcac ctgcagtacc tggaggacac
     3061 cacggcggcg cttgactttg cccaggtgca gcaagcctat gcccagggtc agttccaggg
     3121 aactgcgggg gtcaaggagg cactgtcgct ggggttcacg cgctcggcct tctggctgcg
     3181 gctcgatgtt gccaaccgcg caccctctgc ggccttgcag atgctggagg tggttaacgc
     3241 acgcatctcc tatgtgacgc tgtatgcgcc cggcccggag ggccgctatc aggaaacggc
     3301 cacgggtggc gacatgccgt ttgccacgcg cgcctatgcc aaccgcaact tcgtgtttcc
     3361 aatggcggtg ccggccggta ccacgcagac ggtgtatctg cgggtgcagt cgaccatcgg
     3421 cctgctggtg ccggtgcagc tctgggcaca gaccgacttt gcgcaccatg agcgaaacga
     3481 ctacatggtg caggctttgt acctgggtgt ggcactggcg atggccgtgt ttaacctgct
     3541 gctgtttgtc accctgcggg accgggccta tgccttatat gtgatttttg tgggcttttc
     3601 ggtgctggcg ctgggccaga agacgggctt ggcgtccgag tacctgtggc cgcagtccct
     3661 cacctgggtc aacccgaatt actacacggc cacttcgtgg gggctggtgt gttttatggc
     3721 tttcatgcgc agcatgttgc gcacccccca gaatatgccc cgcattgacc gcgccatgcg
     3781 tgcgggcatg tggctgcatg cgctgatgcc gttgctgtac tggacggcgg tgcagtccgt
     3841 ggcgcgcgcc acgatcatgc tgtatatcgt gtcgtgcctg gcgctgttcg tgttggtggg
     3901 ttggtgcatg tacaagcgga tgcgcgccgc ctatttcttt ggtgcggcgt ttgcggcgct
     3961 gatgacgggc tccctgatga cgctgttgcg cgcgctgggg gtgttgccca ccaatgcgtt
     4021 tacggtcgat ggattgcagg ccgggtccac cctggaaatg ctgttgctgg catttgcgct
     4081 ggcagaccgg tacaacaccc tgcgcaaaga caagctgcag gcccagagcg atctggttct
     4141 tgccaaggaa aagctggtgg aaaccttgca ggcctccgag cgcctgctca cgcagcgggt
     4201 ggaagagcgc acgcagcagc tgcaggtact gaacgaaaag ctcgaagccc tgacgctggt
     4261 ggacgacctg accggcgtgg ccaaccgccg ccagtttgac cagacgctgc acaaggagtg
     4321 gctgcgcatg gagcgcctgc accagccgct ggcgctgctg atgctggatg tggactggtt
     4381 caagccctac aacgaccact acggtcacca ggcgggcgac acctgcctgc gcgctgtggc
     4441 cacggccatt gcagccttaa gccgcaccag cgacctggtg gcgcgctatg gcggtgagga
     4501 gttcgtactg atcgtgcccg ccaccgacag cgagagtgcc atggccctgg cgcgccgtgc
     4561 ctgcgaggcg gtgcaggccc tggcgctgga gcatgtgcgc tcggacaagg gtgtcatcac
     4621 catcagcgtg ggggtggcgg cactggtgcc tggcactggg cacacggtgg aagagctgct
     4681 gcgcgctgcc gacgccgccc tgtaccgcgc caagaaacag gggcgcaacc aggcggtact
     4741 agcccacgat ttgtagtcaa attgacaccc agcccgcgnn nnnnnnnncg actcctgaaa
     4801 ccaagagcgc aagcctgcgc ccgattcctg ctttcatctt tgccagccgc tggctgcagc
     4861 tgccgctgta cctgggcctg attgcggcgc agggcgtgta cgtgttccac ttctgggtgg
     4921 agctggtgca cctgctggaa gccgcttttg gtagccagac cgcgctgcag gcgctggtgg
     4981 acagcattgg ctacaaggcc gatgcacagg tgacgcacct caacgagacc atcatcatgc
     5041 tggtggtgct ggcgctgatt gacgtggtga tgatttccaa cctgctgatc atggtgatcg
     5101 tgggcgggta cgagaccttc gtgtcccgca tggacctcga aggccacccc gaccagcctg
     5161 agtggctggg ccatgtgaac gcctcggtgc tcaaggtcaa gctgggcctg tccatcatcg
     5221 gcatcagctc catccacctg ctcaagacgt ttatcaatgc cgccaattac agcgagaagg
     5281 tgctgatgtg gcagaccatc atccacgtga ccttcctgct cagcgcactg gccattgcgt
     5341 acaccgacaa gctgctgacc tccaaccagt catcccactg atggattact cggcggtgaa
     5401 ctggctggcg ttcttgccct tgaagggggc gtagaacgcc ttgatcttca ccatgtcggc
     5461 ctcgatgtcg ccagtgggca tgaacacggg gccgaggccg ctgatcttct tctcgtagtc
     5521 catgtaggcc atcacgatcg gcaccttggc acccagcgcg atgtagtaga agccggtttt
     5581 ccagtagcgc accttgctgc gcgtgccctc cggcggcacg atgagctgca ccgggccgtc
     5641 ggcggccacg atggcttcca ccgatgccgc caccatgttg gtggcctgct gccggttgac
     5701 cgaaatgccc cccagccatt gcatcaggcc acgaaacggc ggtttgaaga tctgttcctt
     5761 gcccatccag tagatgttca ggcgcagcgc aaacgccacc atcagggtgt tgggcaggtc
     5821 ccagttgctg gtgtgtggcg cggcaatgaa cacactcttg gcgcattcgg ggggcaggct
     5881 gccctgcact ttccagccag tgagtttgag gtacgtcacc gagaaggcgc gcagcagggt
     5941 gttgaccacc ggggtggtga agatggttct atgcatggat ggggtggccg cagggggccg
     6001 gtaacttagc ggggagaggg cggagtgggt acgtggtcca ggtccggtcg gtgggccggg
     6061 tccacatggg tcatgaggtt gagtacgcgg tggcgttgca gcacggcctg gcgggcggcc
     6121 acggcaatgt tgtggcccgc ttcgacggtg atgttggcgt ccacttcaag gtgggcatcg
     6181 accacgatca tgtcgcccat cttgcgggtg cggatgtcgt gcacgccggc cacgccgggg
     6241 gtggaaacca gggtctggcg gatggcctgt acttcctgtt cgtccacggc gcggtccatc
     6301 aggtcgtgca gcgcgtccca gccaaagctc cagcccatct tggcgaccat gaagcccaca
     6361 atcagcgcgg caatcgggtc gagcagcggg tagcccatga ggttgccgat cagtccgatc
     6421 gccaccacca gggacgaggc ggcgtctgac cgcgcatgcc aggcgttggc caccagcatg
     6481 ctggacttga ccgccttggc cacacgcagc atgtagcgaa acagcagctc cttgagaacc
     6541 agcgcggcaa acgccaccca cagggcagag gcgtgtaccg tggcgatggt gtcgggcgac
     6601 tgcagttttt gcagtgcgga ccacaacatg cctacgccca ccgccatcag gatcacgccc
     6661 agcgccagcg acgcggcagt ttcgaaacgg tggtggccgt aggggtggtc ttcgtcggcg
     6721 tctttctggc tgtggtggcc ggcgaacagc acgacaaagt cagacaccag gtcggacagg
     6781 gagtggatgc cgtcggcaat cagggcctgg gatttggcca tgacgcccac cacgatctgg
     6841 gtcgtggaca gcaccacgtt gaccgccacg ctgacccagg tgctgcgcga ggccgctgcg
     6901 ttgcgctcct cggcggtgtg ggggctgtgt tcggacgcgt cgtcgatgtc aacaaaattc
     6961 atcagaggtg tccggaagtg gagacagaaa ttatgcggga tgcgccttgc cggcagctga
     7021 atactgcggt cagggttgca ctatcatggt ctgtattatt caacagtgat ataggccagg
     7081 ggatgcgtac tatgaaaaag cactggtggc tggcgctgct ggcagcgctg gcggtggtgg
     7141 cggtgcagtc cgaaaactcc catacgggtg ggcttcatag gcggcttgtc cgaccgcggc
     7201 tcggatgtgg gtgaaggcgg tcgcaatggt ctgatgctgg ccatcgaaca gcgcaatgct
     7261 gctggcggca tcctcggccg ccccatcgag ctgctggtgc aggacgatgg acagaattcc
     7321 gtgcaggcca tggaagccgt ccgcgctttg gtggacgcca atgtggatgt ggtggtggga
     7381 ccctttacca gtgccatgac cgcggccgtg ttgccccaga tgaaccaggc gcgcgtggtg
     7441 atcatcagcc ccacgatcgc tggcgaggag ttcgtcggca aagacgatta cctgttccgc
     7501 atcaatgccg ccgcggtgac gaacgccggc aattacgcgc agatgctgaa gctgcgcggt
     7561 gtgcacaagg tgggtctggt gtatgacctg gacaaccgca agttcaccga gtcctggtcc
     7621 aaggcgttcg aggcggcttt tgcatcgatt ggggggcgcg tggtcgctgc agtgccgttt
     7681 gaatccaacc gcgagccggc ctttggcgcc ctggtggcaa aagcgctggt gggcaagccc
     7741 gatggcctgg tgtttttggc tactccggtg gacaccgcac gcctggcgca gcaatccagc
     7801 aagatggccc cgggattggc aatggctacc tcggagtggg cagcgtccga gaatctgctg
     7861 gagctgggcg ggccggcggt ggaaggcatg ctgaccttgc gggtctacaa ccgcgacgat
     7921 ccgtcggagc gcacgcgcct gtttctggag gcctaccagg cccgtttttc ccgcgccccg
     7981 ggcgacagcg cggtggctgc ctatgacgcg gcaactgcgt tgatggaagc actggttcgc
     8041 aagtcttctg gcgaaagcct gaaggaggcc ctgattcgct atgggccgtt cgagggcttg
     8101 cagcagccca tcctgttcga cgcgaatgga gacaccaatc gcaccatgta cttcaccgaa
     8161 atccgtgcgg gccggtatgc cctggtgaaa tgatgaacac tcttgcgcgc agctggcgct
     8221 tgcgcagtgt gctggtgggc ctggtggccg ccaccattct gctgacgttc acgctggtag
     8281 gcggcaccat cctgctggtc cagattccga acctcaagcg cgaagcccgc gccgatgtgg
     8341 cgcgcgaagc ggctgaagtg actgcccgtg tagagaccac gctggggacg ctggagtccc
     8401 gtctggaatt tctgggccag acgctgcaga ccggggctac gatgcagcag tccaatgcca
     8461 tgctggaccg cgccctgggt gccgggcctg ccatccgggc gatctacctg gtgtcgctca
     8521 aaggcaaggt gctggcggct ggtctggccg ccgagcagcg tggcttgcgc gccgatctgc
     8581 tgggcagcga tgtgtcggcc acgcggctgt tccgcgaagt ggtgcaacgc cgccggctgg
     8641 tgtgggggga caagtacctg tccgcactga gcggggcggt gacggtgggc gtggccatgc
     8701 cggtggactc ggacctggtg ttgatggcgg agataccgat ttcctacctg ctgagcacgg
     8761 tgcaactggc cataggtggc aagaactcct cggtctggct ggtggaccgc gcgggggaag
     8821 tgctggtgga caccatgggc gggcgcttcg tcaatacggt caacatgctc aacctgccgg
     8881 tggtgcaggc ggccgtcgct gggcgggaac tgccgcgcga gtttgagatg gcgggccgca
     8941 gctactaccc tggtgtttcc cgttccggca aactggactg gtatgtcgtg gcgggtattc
     9001 cggccgggct ggagaacagc cgtatccgcg cgaccgtgat ctacgttgcg ggtggatttg
     9061 ttggctcgct gctggtgggc ttgttgctgg cgccactgtg gtccgcgcgc ctgacccagg
     9121 cgctgacacg tatcgtgggc cgtgccgacc agatcacacg cggcgaggtg gccgggcaat
     9181 ggccgcgcag cctgatcacc gaattcaacc agatctccac cgatctggaa aagatggcga
     9241 ccggactgca ggagcgcgag cagaaattgc aggctgtttt caacacggcg cccgtggcca
     9301 tgtcgctcac cgacatggac aaccactcgg tgttgatcga cgtgaatgag gcctggtgcc
     9361 gcacctttgc acgccgccgc gaagaggcat tgggccgcag tggcgtggag ctcggtctgt
     9421 ggcgctccct ggaggaacgc gatgccgccg tgcagcgcag cgtgaccact ggcggcatgg
     9481 tagagacctg ggccatgcgc ggcgacgggg tgcccatcct gtgccaggcg ttccgtcgcg
     9541 agttgcgtct ggaagacgcc aacctgatga tctgggcctc ggtggacatc accgccgtgc
     9601 gccgcaccga ggatgccctg cgcgacctca atgccgatct ggaggctcgg gtggccagcc
     9661 gcacccggca gctggaaaaa ctcaacaccg agctgtcggg tgctttcgag aatctgcgca
     9721 tgacacaggg tgagctggtg cggtcagaaa agatggcggc gctgggtagt ctggtggcgg
     9781 gtgtgtcgca cgaactcaat acgccgctgg gcaacgggtt gatggcggtg tccgcgttgg
     9841 ctgatgcagt gcgcagcttc aagggaacca tgcagtcggg tctgcgccgc tccgacctgg
     9901 aggacttcgt gctccgcgtg gaccagggtg cagacattgc cgtgcgcaac ctgcagcgtg
     9961 cggccgatct ggtggtgagc ttcaagcagg tggcggtgga ccagaccagt tcgcagcggc
    10021 gccgctttga gctgtcggaa gtggtgcacg agatggtgat cagtctgggc cccaccttgc
    10081 gtcgcacgcc gtatgaagtg gtgctggatg ttcccaaaat cggcctgctg ctcgacagct
    10141 atccgggccc gctggggcag acccttggca acctgatcaa caacgcggtg atgcacgcct
    10201 tcgatggccg ccagaacggc acgatccgca tcaccggcta ccgcgatgtc aacggctgga
    10261 ttgccctgcg ggtggaggac aacgggcagg gcattcccgc cgccctgctg ccgcgcattt
    10321 ttgatccgtt tgtgaccacc aagatgggcc gcggcggcac cgggctgggt ctgcacattg
    10381 cctacaacgc ggtgaccacc ctgctgggtg gaacgctgac ggttcacagc gtccaggggc
    10441 agggcaccac cttcgagctg cacctgccgg agcaggcgcc cctggcggtt tagctcaggt
    10501 gcttgccggc aatgccggcc agttcaaaca tcgtgacctg gggcttgccg aacacggcca
    10561 gcagcttggc atcggcgttg atgctgcgct tgtccttggc gtcctgcagg ccgttggcct
    10621 tgatgtagtc ccacagcttt ttcatgacct ggggccgggc aatcagctca ggcccaatca
    10681 ctgcagccag ttcggcgctg ggggtcttgc cgctggctgc ggtggtcttg cgcggtgcct
    10741 tgggcttggc caccttggcg gccgcggttt tgctggctac ggctttcttt gcaggtgctt
    10801 ttttggctgt agcccccgtg gatgctgcgc gagcagctcc tgtttttgta gcagttgtct
    10861 tggctgcggt ctttgcagcg gtcttgcgcg gcgggaactt gctgggcgca aactcaaagt
    10921 tcaccttgcc ggcttccggg tcccaggcca gcatggcctt gaaggcgcgg cgggtgcgca
    10981 tggagacaaa cttttccagc atgtcggtct tgccggtggc cagcagcttt tgcatctgct
    11041 cgcgctcaat gggctgctgc aggatgatct ggccgctctt gaagtcgcag ctgggtgtgg
    11101 ggtgttcgtg ggtgggcacc gagcggtcgc acacatagtt cttgccgtgc tcgaacacat
    11161 ggccgccgca cttggggcag gtgcccagcg actctttgcc gctgaagtcg atgatctcgc
    11221 cggactcttc gcctgccttg tcgtcgccaa agtcgaattc gagcttgtag ttcttggtgt
    11281 cttcgtcgaa cttgatcacc atctcggcca caaacggcca gccggccttg gagcggaagc
    11341 cgtccagcgg gccgatgtgc ttgtcgcgca ggaactgctc gacttcggcc tgctcaaagg
    11401 tgcggcccgc gggtgatttg ccaaacgaga agccgcagcc ctcttcggcg ccactcttgc
    11461 cggtgcaggt gtagcggcgg tagttttctt tcacgatgcc gccgcagttc gggcaggggc
    11521 tgctgagcgt ggcgtagtcg ccggggatgg tgtcgcggtc gtattccttg gccttcttga
    11581 ccatgcgctc ggtcatggag gcaatctcgg ccatgaaggc ctcgcggctc atcttgccgt
    11641 gctccatctg gttgagcttg tattcccatt cgccggtcag ct
//
LOCUS       DAOSJM010000022        11479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5596_length_11479_cov_4.741159, whole genome shotgun sequence.
ACCESSION   DAOSJM010000022 DAOSJM010000000
VERSION     DAOSJM010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11479
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5596_length_11479_cov_4.741159"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /gene="trpA"
                     /locus_tag="PKX82_01585"
     CDS             complement(<1..570)
                     /gene="trpA"
                     /locus_tag="PKX82_01585"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOF50966.1"
                     /translation="MSRIASKFADLKTDGRKALIPFVTAGYPFADVTPELMHGMVAGG
                     ADVIELGVPFSDPSADGPVIQKAGDKALAFDIGLVQVLAMVREFRRTDTTTPVVLMGY
                     ANPVERYDQKHAKAGAEAMSGSPFVQDAAAAGVDGVLIVDYPPEECEAFAADLRAHHM
                     DLIFLLAPTSTDARMAQVARVASGYVYYVS"
     gene            complement(567..1865)
                     /gene="trpB"
                     /locus_tag="PKX82_01590"
     CDS             complement(567..1865)
                     /gene="trpB"
                     /locus_tag="PKX82_01590"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOF50967.1"
                     /translation="MFDYHQPDPSGHFGKYGGSFVSETLTHAINELKDAYAKYQHDPE
                     FLAEFKSELAHFVGRPSPVYHAARMSREMGGAQIFLKREDLNHTGAHKINNTIGQAML
                     AKRMGKPRVIAETGAGQHGVATATICARYGLECVVYMGSEDVKRQSPNVYRMKLLGAT
                     VVPVESGSKTLKDALNEAMRDWVANVDNTFYIIGTVAGPHPYPMMVRDFQSVIGNECL
                     VQMPEMLKTAGCTSAQPDAVVACVGGGSNAMGIFYPYIQHDATRLIGVEAAGEGLESG
                     KHSASIQKGTAGVLHGNRTYVLQDDNGQVTETHSISAGLDYPGVGPEHAFLSDIGRAE
                     YVGITDKEALEAFHYLCRTEGIIPALESSHAVAYAMKLAKTMRPDQSILVNLSGRGDK
                     DIGTVADLSNGEFFCRPSCTGQSVKGGEQPVKLVKNGGAA"
     gene            complement(1866..2573)
                     /locus_tag="PKX82_01595"
     CDS             complement(1866..2573)
                     /locus_tag="PKX82_01595"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOF50968.1"
                     /translation="MTLTKTRIKICGLTREQDVDAAVAAGADAIGFVLYAKSPRAVTP
                     QRAAELTRRLPPFITPVLLFVNATATEIIAACAMIPGATIQFHGDESPQDCLVATAEG
                     ARPFLRAARIPLGEGAAHFDLVKYAQDFSAAQAILLDAHVDGYGGGGKTFNWSLLPPN
                     VNAHLVLSGGLTPANVGDGITSVRPRCKTLAVDVSSGVEATGADGTPLRGIKDPEKIK
                     QFVAAVRAADQQLPGLK"
     gene            complement(2570..3352)
                     /gene="truA"
                     /locus_tag="PKX82_01600"
     CDS             complement(2570..3352)
                     /gene="truA"
                     /locus_tag="PKX82_01600"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOF50969.1"
                     /translation="MRVALGISYNGQAYQGWQSQSSGQTIQDQLEKALGKFAAQRVST
                     VCAGRTDAGVHGIMQVVHFDTPLERDTYSWVRGTNANLPRDIAVQWAVITPESFHCRA
                     CASSRRYAYVLYESQIRPSVDQGRVGWTFHTLNLAAMQEAADILLGEHDFTSFRASQC
                     QALSPVKNMLEIAFSKRGSYWRIEFEANAFLHHMIRNIMGCLVEIGKGRKPPQWMAEV
                     LAARDRRAAAPTFSPDGLYFLGPRYDAKWGIPDRAPAYDGLP"
     gene            complement(3400..5844)
                     /locus_tag="PKX82_01605"
     CDS             complement(3400..5844)
                     /locus_tag="PKX82_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HOF50970.1"
                     /translation="MPEINAEEAASLRASVASPATFVAAGLDYNPAMSGLQTSLQRRP
                     DGRAYIRLSSDRSINDPFVDMILEATWSSGRIVRDYTMLFDPPVLKQAAAPTQAQVPA
                     QQSVSAPAKAAPAPATSATSNTSRNVASSKPARVAPVASAAAPASKAAAEGGQQVVVK
                     SGDTASKIAARGVTTGVSLDQMLVAMMRSNPDAFVNNNLNRLRTGAVINMPSADQAQS
                     VAPGEASQMVIAQSKDFNDFRRNLASNAPKADVPPADRAASGKVDAKVEDKKPTAATP
                     DKLTLSKGSVQSKADEAKIAKERADKDAAARAAEIAKNISDLNKLGAASAPKTAPAAT
                     VAPAPAVAASKPVVAPAVTVATAPPVAVPASAPAVAASAPVAVASAPAPTASAVAKPK
                     PAPAMPVPAEPAGLLDELLEDPMVPAAAGGLVALLGGLGFYFMRKRKQSSARVDSSFL
                     ESRLQPDSFFGASGGQRIDTNDSPGNPSSMVYSTSQLDAADDVDPVAEADVYLAYGRD
                     LQAEEILKEAVRHSPGRLAIHTKLLEIFAKRRDVSNFQSSAQQALNLTGIDSPEWARI
                     CEMGLTIDPENRLYQPGGATASAFTPAADAGQTVPGSVFGVTVAESSNAELPSSASAG
                     VDLDLDLDFSIDDEPGSAISDVTGARVPAREQTVKMEAAADDGHMALDFDISGPAELD
                     ASPGMQEYARTNSGQVPLTESSEPLPSFEPTGTLPVEAAAPSPMAGNDAGMLEFDLGS
                     LSLDLDTPATSAADAPDSTSSIAGDDPLETKLALAEEFISIGDEDGARALLEEVVAES
                     SGDMRAKAQRVLANLS"
     gene            complement(6203..7330)
                     /gene="asd"
                     /locus_tag="PKX82_01610"
     CDS             complement(6203..7330)
                     /gene="asd"
                     /locus_tag="PKX82_01610"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOF50971.1"
                     /translation="MKLVGLVGWRGMVGSVLMDRMQAEGDFGLIEPLFFSTSNAGGKA
                     PSQAKNETTLQDAFNIDALKRCDIIISAQGGDYTTEVFPKLRSAGWNGHWIDAASTLR
                     MEKDAVIILDPVNMPVIKDALSKGGKNWVGGNCTVSCMLMGVGALYKAGLVEWMSTQT
                     YQAASGGGAQHMRELLTQYGTLNAEVKALLDDPKSAILEIDRKVIAKQRALTGAETAN
                     FGVPLGGSLIPWIDKDLGNGQSKEEWKGMAETNKILGMGEDFNSAAIPVDGFCVRVGA
                     MRCHSQALTFKLKKDVPVADIEAMIAADNQWVKVVPNTREATIKDLTPVAVTGTMTIP
                     VGRVRKLAMGPEYVGAFTIGDQLLWGAAEPLRRMLRILLDA"
     gene            complement(7404..8507)
                     /gene="leuB"
                     /locus_tag="PKX82_01615"
     CDS             complement(7404..8507)
                     /gene="leuB"
                     /locus_tag="PKX82_01615"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOF50972.1"
                     /translation="MKIAVLPGDGIGTEIVAEAVKVLNVLDLPLEMESALVGGAAYEA
                     HGHPLPESTLKLAKEADAVLFGAVGDWKYDTLDRPLRPEQAILGLRKNLGLFANFRPA
                     ICYEQLVGASSLKPELIAGLDILIIRELTGDIYFGQPRGRRTAVDGHFPGSEEAFDTM
                     RYSRPEIERIAHVAFKAARTRKAAGTEGRVTSVDKANVLETFQFWKDVVTEVSKEYPD
                     IALDHMYVDNAAMQLVKAPKRFDVVVTGNMFGDILSDEASMLTGSIGMLPSASLNASN
                     QGLYEPSHGSAPDIAGKNIANPLATILSAAMMLRFSLNQAQAADRIESAVKDVLASGL
                     RTADIWSEGTAKVSTREMGDAVVAAITKTITKG"
     gene            complement(8542..9192)
                     /gene="leuD"
                     /locus_tag="PKX82_01620"
     CDS             complement(8542..9192)
                     /gene="leuD"
                     /locus_tag="PKX82_01620"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOF50973.1"
                     /translation="MKSFTVHKGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGQNLFD
                     EWRYLDAGFPGQDPTSRKPNPDFVLNQPRYAGASILLARKNFGCGSSREHAPWALDQY
                     GFRAIIAPSYADIFFNNSFKNGLLPIVLGESLVDQLFNEALAFPGYALTIDLERQVIV
                     KPSGDEIPFDVQAFRKYCLLNGFDDIGLTLRHADKIKAFEAQRLAQKPWLAHSMQA"
     gene            complement(9192..9320)
                     /locus_tag="PKX82_01625"
     CDS             complement(9192..9320)
                     /locus_tag="PKX82_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HOF50974.1"
                     /translation="MKTLISLLCVTSFALLAGCNTVKGMGQDLQKAGEKIENSVKK"
     gene            complement(9341..10804)
                     /gene="leuC"
                     /locus_tag="PKX82_01630"
     CDS             complement(9341..10804)
                     /gene="leuC"
                     /locus_tag="PKX82_01630"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOF50975.1"
                     /translation="METQTHARTLYDKLWDDHVIHTEEDGTAILYIDRHLVHEVTSPQ
                     AFEGLREAGRKVWRVSSIVATADHNTPTTGWDRGYDGITDPTSKEQVTTLDSNIREFG
                     SAAYFPFLSKRQGIVHVIGPENGAMLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEH
                     VMATQTLLAKKARNMLVKVEGTLSKGCTAKDVVLAIIGKIGTAGGTGYTIEFGGSAIR
                     ALSMEGRMTVCNMAIEAGARAGLVAVDEKTIEYVKGRPLAPGHAGVAGGAAAVEWDQA
                     VTYWKTLHSDTGAKFDTVVELDATQIVPQVTWGTSPEMVLGIDGHVPDPDKEKDPNKR
                     SAIERALVYMGLEPGKALDDLFIDKVFIGSCTNSRIEDMRDAAAVVKRLGQRVAKNVK
                     LAMVVPGSGLVKEQAEREGLHEIFKAAGFEWREPGCSMCLAMNADRLEPGERCASTSN
                     RNFEGRQGAGGRTHLVSPAMAAAAAIHGHFVDVRKFS"
     gene            complement(10923..>11479)
                     /locus_tag="PKX82_01635"
     CDS             complement(10923..>11479)
                     /locus_tag="PKX82_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOF50976.1"
                     /translation="PFAAIAAGVACLWGPAHGGANEAALNMLGDIQKNGGVEKIGEFI
                     KAVKDKNSGVKLMGFGHRVYKNYDPRAKLMQETCNEVLAELGLENDPLFKLAKELEKI
                     ALEDDYFVQRKLYPNVDFYSGIVQRAIGIPVNLFTGIFALARTVGWIAQLNEMIGDPE
                     YKIGRPRQLFTGSVKRDVKPVGQR"
BASE COUNT         2115 a         3331 c         3584 g         2449 t
ORIGIN      
        1 cgacacatag tacacatagc cactggcgac gcgcgccacc tgggccatac gcgcatcggt
       61 gctggtgggt gccagcagga agatcaggtc catgtggtgc gcgcgcaggt cggcggcaaa
      121 ggcctcgcac tcttcgggcg ggtagtcgac gatcagcacg ccgtcgacgc cagcggctgc
      181 ggcgtcctgg acgaaggggc tgcccgacat ggcctctgca cctgcttttg catgcttctg
      241 gtcgtagcgc tccaccgggt tggcatagcc catcagcacc acgggggtgg tggtgtcggt
      301 cctgcggaac tcgcgcacca tggccaacac ctgcaccaga ccaatgtcaa acgccagggc
      361 cttgtcgccg gccttctgga tgacagggcc gtcggcgctg gggtcggaga agggaacgcc
      421 cagttcgatg acgtcggcac cgccagcgac catgccgtgc atcaactcgg gtgtgacgtc
      481 ggcgaagggg tagccggcgg tcacgaaggg aatcaatgcc ttgcggccgt ccgttttgag
      541 atcagcaaat ttggaggcaa tgcggctcat gctgcacccc catttttcac cagcttgaca
      601 ggttgttcac cacccttgac cgattgcccg gtacagctgg ggcggcagaa aaattcgcca
      661 ttgctcagat cggctaccgt gccaatgtcc ttgtcgcccc gtcccgacag gttgaccaga
      721 atcgattggt cgggtcgcat ggtcttggcc agtttcatgg cgtaggcgac ggcgtggctg
      781 gattccagcg cggggatgat gccctcggta cggcacaggt agtggaaagc ttccagtgct
      841 tccttgtcag tgataccgac gtactcagcc cggccgatgt cagaaaggaa cgcatgttca
      901 gggccaacac cggggtagtc caggccggcg ctgatgctat gggtttcagt gacttggccg
      961 ttgtcgtctt gcagcacata ggtgcggttg ccgtgcagca cacccgcagt tcccttctgg
     1021 atgctggcgg agtgtttgcc actctccaga ccctcgccag ccgcttccac cccgatcaga
     1081 cgggttgcat cgtgctggat ataggggtag aagatgccca tggcattcga gccaccgccc
     1141 acgcaggcga ccacggcatc cggctgcgcg ctggtgcagc cagcggtctt gagcatttcc
     1201 ggcatctgca ccagacattc gttgccaatc acgctttgaa aatcccgcac catcattgga
     1261 taggggtgtg gcccggctac ggtgccgatg atgtagaacg tgttgtccac gttggcaacc
     1321 cagtcgcgca tggcttcgtt gagtgcatcc ttcagcgtct tgctgccgct ctccaccggc
     1381 accacggtgg ctcccagcag tttcatgcgg tacacgttgg ggctttggcg cttgacgtct
     1441 tcgctgccca tatagaccac gcattccagc ccgtaacggg cgcagatggt ggcggtcgcc
     1501 acaccgtgct ggccggcgcc ggtttctgca atcacgcgcg gcttgcccat gcgcttggcg
     1561 agcatggcct ggccaatggt gttgttgatt ttgtgggcgc cggtgtggtt caggtcttcg
     1621 cgcttgagaa aaatctgcgc gccacccatc tcgcggctca tgcgtgctgc gtggtacacg
     1681 ggggaggggc gtccgacaaa gtgtgcaagt tccgacttga attctgcgag gaattcgggg
     1741 tcgtgctggt acttggcgta ggcgtccttc agttcgttga tggcgtgggt cagcgtctcg
     1801 gagacaaagc tgccgccgta tttgccaaag tgaccgctgg ggtcgggctg gtggtaatca
     1861 aacatctatt tcaatcctgg aagttgttgg tcggcggcgc gcacggcggc gacaaactgt
     1921 ttaatttttt ccgggtcctt gatgccgcgc aacggagtac cgtctgcgcc ggtggcttca
     1981 acgcccgagc tcacatcgac ggccagcgtt ttacagcgtg gccgtaccga cgtgatgccg
     2041 tcgcccacgt ttgcaggtgt gagtccacca ctcaagacga ggtgagcgtt gacgtttgga
     2101 ggaagaagtg accaattgaa tgttttgccg ccccccccat acccgtccac atgggcgtcg
     2161 agcaaaatgg cctgggcggc tgaaaagtcc tgcgcgtatt ttacgaggtc gaaatgcgcg
     2221 gcgccttctc ccagcggaat acgggctgca cgcaggaaag ggcgggcacc ttctgcagtc
     2281 gccaccaggc agtcctgggg gctctcatct ccatgaaatt ggatggtagc ccccggaatc
     2341 attgcgcagg cagctattat ttctgtagca gttgcattga caaacagcag cacaggagta
     2401 atgaaaggag gcaggcgcct ggtcagttcg gcagcccgtt ggggtgtgac agcgcgcgga
     2461 cttttggcat acagaacaaa gccgatggcg tcggccccgg cagcaaccgc tgcatccacg
     2521 tcctgttcgc gcgtcagtcc gcaaatcttg attcgggttt ttgtcagtgt catggcaggc
     2581 catcatacgc aggcgcgcgg tcggggatac cccatttggc gtcgtagcgt gggcccagaa
     2641 agtacaagcc atcgggggag aaggtggggg ccgcggcgcg gcggtcgcga gcagcgagca
     2701 cttctgccat ccattgaggt ggttttcggc ctttgccgat ctcgaccagg cagcccatga
     2761 tgttgcggat catgtggtgc aaaaaggcgt ttgcctcgaa ctcgatgcgc cagtacgagc
     2821 cgcgtttgct gaaagcaatc tccagcatgt tcttgacggg tgacagggcc tggcattgcg
     2881 aggcccgaaa agaggtgaag tcatgttcac ccagcaggat atcggcggct tcctgcatgg
     2941 cagccagatt cagcgtgtga aaagtccagc caacccggcc ttggtccaca ctggggcgaa
     3001 tctgtgattc gtacagcacg taggcatagc gccgggaact ggcgcaggcc cggcagtgga
     3061 acgactcggg tgtgatcacg gcccactgca ccgcgatgtc gcgcggaaga ttggcattgg
     3121 tgccgcgcac ccaggaatag gtgtcgcgct ccagcggggt atcaaagtgc accacctgca
     3181 tgatgccgtg cacaccggcg tcggtgcgtc cggcgcagac cgtggacacg cgctgtgctg
     3241 caaatttgcc gagtgctttt tccagctggt cctggatagt ttgaccagag gactggctct
     3301 gccagccctg gtaggcctgt ccgttgtagc tgatgcccag cgccaccctc atggcaatgc
     3361 ctgcccttca gccacagacc gccatgccct ggtgatactt cagctcaggt tggccaacac
     3421 acgctgcgcc ttggcccgca tgtcgccaga tgactcggcg acaacttcct caagcagtgc
     3481 gcgagcgccg tcttcgtctc cgatggagat aaattcttcg gccagggcca gcttggtttc
     3541 cagcggatca tcgccagcaa tcgaggaggt cgagtccggt gcgtcggctg cagatgtggc
     3601 tggtgtatcc agatccagcg acagggagcc cagatcgaat tcgagcattc cagcgtcgtt
     3661 gccggccatc ggggagggcg ctgcggcttc cacaggcaag gtgcctgtgg gttcgaacga
     3721 aggcaagggt tccgaagact ctgtcagtgg cacctggccg ctgttcgtcc gcgcatattc
     3781 ctgcataccc ggggacgcat ccagctcggc agggccggaa atatcaaagt ccagagccat
     3841 gtggccgtcg tcagcggcag cttccatctt gacggtctgt tcacgcgcag gcactctggc
     3901 accggtcacg tcgctgatgg cactaccggg ttcgtcgtca atggagaaat ccaggtcgag
     3961 gtccagatca acgccggcag acgcagaaga gggcaattcc gcgttggagg actcggcgac
     4021 ggttacgcca aataccgagc ctggcacggt ctgccctgcg tctgcagctg gcgtgaatgc
     4081 actggcggtt gcgccgccgg gctggtacag ccggttctca ggatcaatag tcaagcccat
     4141 ttcgcagata cgcgcccact ccgggctgtc aatgccggtg aggttcagtg cttgctgcgc
     4201 gctactctgg aaattggata catcgcggcg tttggcaaaa atttccagca acttggtgtg
     4261 gattgccagg cgtcccgggc tgtggcgcac tgcttctttc agaatttcct ctgcctgcag
     4321 gtcacggcca taggccagat acacatcggc ttcagccact ggatcgacgt catccgcagc
     4381 atccagctgg ctcgtcgaat acaccatgga ggacgggttg ccgggactgt cgttggtatc
     4441 gatgcgctgg ccaccgctgg caccgaagaa ggagtcgggc tggaggcggc tttcgaggaa
     4501 cgaactgtct acgcgcgcgg agctttgttt gcgtttgcgc atgaaataga aacccagtcc
     4561 acccagcagt gcgaccagtc cgcccgcagc ggcgggcacc atcgggtctt ccagcaactc
     4621 atccagcagt cccgcgggtt ccgcaggcac tggcatcgca ggggcaggtt tgggtttggc
     4681 cacagccgac gcagtgggcg ccggggcgga tgccacagcg actggtgcag aagcagcaac
     4741 tgccggagct gaggctggca cggcgacggg tggcgctgtt gcaacggtga cggcaggcgc
     4801 cacaacgggt ttggatgccg caactgcggg tgccggggcg actgtcgcag cgggcgctgt
     4861 tttgggtgcg ctagctgcac ccagcttgtt caggtcactg atgtttttgg cgatttcggc
     4921 tgcacgtgcc gctgcatcct tgtctgcccg ctctttggca atcttggctt catctgcctt
     4981 gctttgcacc gagcccttgg agagggtcag cttgtcaggc gtggcggcag tgggcttctt
     5041 gtcttccact ttggcatcca ctttgccgct ggctgcacgg tccgcggggg gtacatccgc
     5101 cttgggtgca ttgctggcca gattgcggcg gaagtcgttg aagtccttgc tttgggcaat
     5161 gaccatctgt gacgcttcgc cgggtgcaac ggactgcgcc tgatctgcgc tgggcatgtt
     5221 gatgacggca ccggtgcgca aacgattcag attattgttg acaaaggcgt ccgggttgga
     5281 tcgcatcatg gcgaccagca tctggtcgag cgacacgcca gtcgttacac cgcgcgcggc
     5341 aattttgctc gcggtatccc cgctcttgac gacgacttgt tgaccgcctt ctgcggctgc
     5401 tttggaagcg ggggcggcgg cagaagccac cggtgccacg cgtgctggct tgctggaagc
     5461 gacgttgcga gaggtgttgg aagttgcgga agtcgctggc gctggtgccg cttttgctgg
     5521 agccgacacc gactgttgcg caggcacctg cgcctgtgtg ggcgctgcag cctgtttgag
     5581 gaccggcggg tcgaacagca tggtgtagtc ccggacaatc cggccagagg accaggttgc
     5641 ctccagaatc atgtcgacaa atggatcatt gatcgaacgg tcgctgctca ggcggatgta
     5701 ggctcgtcca tcagggcgac gttgcaggct ggtctgcaag cccgacatgg ctgggttgta
     5761 atccaggcca gcagcgacga aggtggcggg agaggccacg ctggcacgca gggatgcagc
     5821 ctcttcggcg tttatttccg gcacatcaat ttcggcacgc agtggttcgc ccagtgccga
     5881 caaaaccgta agccggccaa gcgaaagcgc agaagccccg gtagaagtga aaccgagtag
     5941 cgcaatggct gcggccgcaa tagcggtctg ttgccaacga tgtgacttag aaatcaattt
     6001 agtagcctcc ggtgctcagg caagcggcaa tttcttctgg gaaatgcgac gttccagcct
     6061 gactctcaaa acgtaaaaag taccataaca tcaatgtctt agactgacaa gctaagaaac
     6121 cttttaactt tgaaagccgg actggttcgg ggggcaaggt cgtgtcaaac gttgccaagc
     6181 gacaacgacc tgtaaccggt tgttacgcgt cgagcaaaat gcgcaacatg cggcgcaaag
     6241 gttctgcggc accccacagc aactggtcgc cgatggtgaa tgcgcctaca tattccgggc
     6301 ccatggccag cttgcggacg cgtcccacag ggatggtcat ggtgccggtg accgccacag
     6361 gtgtcaggtc cttgatggtc gcctcgcggg tgttcggtac aactttgacc cactggttgt
     6421 cggcagcgat catcgcctca atgtcggcta cgggcacgtc cttcttcagc ttgaaagtca
     6481 gggcctggct atggcagcgc atggcgccca cgcgtacgca gaagccatct acgggaatgg
     6541 ctgcagagtt gaagtcttca cccatgccca ggatcttgtt ggtctccgcc atgcctttcc
     6601 actcttcctt ggattggccg ttgcccagat ccttgtcgat ccagggaatc agcgagccgc
     6661 cgagcggcac gccgaaattg gcagtttcag cgccggtcag tgcacgttgt ttggcgatga
     6721 ccttgcggtc gatttccaga atcgcacttt tgggatcgtc cagcagggct ttgacctctg
     6781 cgttcagtgt gccgtactgc gtcaaaagtt cgcgcatgtg ctgcgcgccc ccaccagacg
     6841 ccgcctggta agtctgggtg ctcatccact cgaccagacc ggccttgtag agcgcgccca
     6901 cgcccatcag catgcagctg acggtgcagt tgccacccac ccagttcttg ccacccttgg
     6961 acaaggcatc cttgatcacg ggcatgttga ccggatccag gatgatgacc gcgtcttttt
     7021 ccatgcgcag ggtcgacgct gcgtcgatcc agtggccatt ccagcctgcc gaacgcagtt
     7081 tgggaaatac ttcggtggtg tagtcgccgc cctgggcact gatgatgatg tcgcaacgct
     7141 tgagcgcatc aatgttgaag gcgtcctgca acgtggtttc gtttttcgcc tgcgatgggg
     7201 ctttgccacc cgcattggaa gtggagaaaa acaggggttc aatcaggcca aagtcaccct
     7261 cagcctgcat gcggtccatc aatacggagc cgaccatccc gcgccagccc accagtccta
     7321 ctaatttcat atcaacgccc tttcaaaaat ttgtttgccc ccagctcgtc ttgccggggg
     7381 agggcgcgac gaacgggagc tgttcagccc ttggtaatgg ttttagtgat ggcggccacg
     7441 acagcgtcac ccatctcgcg ggtgctgacc ttggcggtgc cttccgacca gatatctgcc
     7501 gtacgcaaac ccgacgcaag cacgtccttc accgcggact cgatacggtc agcggcttgg
     7561 gcttggttga gtgagaaacg taacatcatg gcagcggaga gtattgtagc caatgggttt
     7621 gcaatattct taccagcgat gtcgggcgca ctgccgtggc tgggctcgta cagaccctgg
     7681 ttgctggcgt tcaggctggc ggacggcagc atgccgatgg agccggtcag catggaggct
     7741 tcgtcggaca gaatgtcgcc gaacatattg cccgtgacga ccacgtcaaa gcgtttgggc
     7801 gccttgacga gttgcatggc agcgttgtcc acgtacatgt ggtccagggc gatatctgga
     7861 tattccttgc tgacttcggt caccacgtct ttccagaact ggaaagtttc cagcacgttg
     7921 gctttgtcga cgctggtcac acggccctcg gtaccagcag ccttgcgggt acgcgccgcc
     7981 ttgaaagcga catgcgcgat gcgctcgatc tcggggcggc tgtagcgcat ggtgtcaaac
     8041 gcttcttccg agccggggaa gtgaccatcc accgctgtgc ggcgtccgcg gggctggcca
     8101 aagtagatgt ctccggtcag ttcgcggatg atcaggatgt ccaaacctgc aatcagctcg
     8161 ggtttgaggc tggaagcacc gacgagttgt tcatagcaaa tggccgggcg gaagttggcg
     8221 aacaggccca gattcttgcg caggcccaga atggcctgct cggggcgcag aggacggtcc
     8281 agcgtgtcgt acttccagtc accgacggcg ccgaacagta cggcgtcagc ctctttggcg
     8341 agcttgaggg tggattcggg cagcggatgg ccgtgggctt cgtaggcggc accgccgacc
     8401 agggcgctct ccatctccag tggcaggtcc agcacgttca agaccttgac ggcctcggcc
     8461 acaatttcgg ttccaatgcc gtcgcctggc aatactgcaa ttttcatggt gttcttcgtt
     8521 tgctatgaat ttagtagcag cttacgcttg catgctgtgg gctagccatg gtttttgtgc
     8581 caaacgctgg gcttcgaagg ccttgatctt gtcggcgtgg cgcagggtca gaccaatgtc
     8641 atcgaagcca ttgagcaggc agtatttgcg aaacgcctgc acgtcgaatg gaatttcgtc
     8701 gccggacggt ttgacgatga cctggcgctc cagatcgatg gtcagggcat aacctggaaa
     8761 cgccaacgct tcattgaaca actggtccac cagtgactcg ccgagcacga tgggcagcag
     8821 gccatttttg aagctgttgt tgaagaagat gtcggcgtag cttggcgcaa tgatggcgcg
     8881 aaagccgtat tggtccagtg cccagggggc gtgctcgcgg gatgagccgc agccgaagtt
     8941 cttgcgcgcc agcaggatgg aagcacccgc atagcggggc tggttcagca caaagtccgg
     9001 attgggtttg cggctggtgg gatcctgacc ggggaagccg gcatccaggt agcgccattc
     9061 gtcaaacaga ttttgtccga aacccgtctt gcggatggat ttgagaaact gcttggggat
     9121 gatcgcgtca gtgtcgacgt tctcgcggtc cataggcgct acgagaccct tgtgtacggt
     9181 gaatgatttc attatttctt gaccgagttt tcgattttct cgccggcctt ttgcaggtcc
     9241 tggcccatgc ccttgacggt gttgcaacct gcaagcaggg caaagctggt gacgcacagc
     9301 agagagatca gagttttcat ggaatagtcc cgacggctaa tcaactgaac ttgcggacat
     9361 ccacaaaatg tccgtggatg gcggcggcgg cggccatggc cgggctcacc agatgggtgc
     9421 ggccacctgc gccctgacgg ccttcgaaat tgcggttgct ggtggaggcg cagcgttcac
     9481 cgggctccag gcggtcggca ttcatcgcca ggcacatgga gcaaccgggc tcgcgccatt
     9541 caaagccagc cgccttgaat atctcgtgca acccttcgcg ctcggcttgc tccttgacca
     9601 ggcctgaacc gggaaccacc atggccagct tcacattctt ggccacacgc tggcccagcc
     9661 tcttgaccac ggcggctgcg tcgcgcatgt cttcgatgcg gctgttggtg caggagccaa
     9721 taaacacttt gtcaataaac aggtcgtcca gtgccttgcc tggctccaga cccatgtaca
     9781 ccagcgcgcg ctcgatggcg ctgcgcttgt tcgggtcttt ttccttgtcc ggatcaggta
     9841 catggccatc gatgccaagc accatctcag gcgacgttcc ccaggtcacc tgcggaacaa
     9901 tctgcgtagc atcgagttca accaccgtgt cgaatttggc tccagtgtcg gagtgcaggg
     9961 tcttccagta ggtcactgcc tgatcccact caacagctgc tgcaccaccg gcaacaccgg
    10021 cgtggccggg ggccagggga cgccccttga cgtattcgat cgttttttca tccacggcca
    10081 ccagacccgc gcgggcaccg gcctcgatgg ccatgttgca gaccgtcatg cggccttcca
    10141 tgctcagcgc gcggatggca gagccaccga actcgatggt gtagcccgtg ccgccagcag
    10201 taccgatctt tccgatgatg gccagcacca cgtctttggc agtgcagcct ttggacagcg
    10261 tgccttcgac cttgaccagc atgttcctgg cttttttggc cagtagggtc tgcgtagcca
    10321 tgacgtgttc gacttcactg gtgccgatgc cgtgtgccag agcgccaaag gcgccgtggg
    10381 tcgaggtgtg ggagtcacca cacaccacgg tcattcctgg aagcatggcg ccgttctctg
    10441 gcccgatcac gtgcacgatg ccctggcgtt tcgagagaaa cggaaagtac gctgcagagc
    10501 caaattcgcg gatgttgctg tccagtgtcg tgacctgttc cttgctggtg gggtcggtga
    10561 tgccgtcata accacggtcc cagcctgtgg tcggggtgtt atggtcagcg gtcgcgacga
    10621 tggagctgac gcgccagact ttgcgtccgg cttcgcgcag gccctcaaac gcctgcgggc
    10681 tggtgacttc gtgcaccaga tggcgatcaa tgtaaagaat ggcggtgcca tcttcttctg
    10741 tgtgaatgac gtggtcatcc cagagcttgt cgtacagcgt gcgtgcgtgc gtttgtgttt
    10801 ccatgcggac cttgcggtaa ttcttcggca atctgcgatt ttatgctcca cgcggtgcga
    10861 ggggcaggca acaaaaagcc cgccggcgtt gccgtccggc gggcctctgg ttcaaggcgg
    10921 tatcagcgtt ggccgacggg cttcacatcg cgcttgacgg agccggtgaa caactgacgg
    10981 ggacggccga tcttgtactc ggggtcgcca atcatttcgt tcagctgagc gatccagccg
    11041 acggtgcggg ccagggcgaa gatgccggtg aacaggttca ctgggatgcc aatggcgcgt
    11101 tgcacgatgc cggagtagaa gtccacgttc gggtagagct tgcgctgcac gaagtagtcg
    11161 tcttccaggg cgatcttttc cagttccttg gccagcttga acagcgggtc gttttccagg
    11221 cccagctcgg ccagcacttc attgcaagtc tcttgcatga gcttggcgcg ggggtcgtag
    11281 ttcttgtaca cgcggtggcc aaagcccatc agcttgacgc cggagttctt gtccttcacg
    11341 gccttgatga actcaccgat cttttccacg ccgccgtttt tctggatgtc acccagcatg
    11401 ttcagtgccg cttcattggc accaccgtgt gccgggcccc acagacaggc cacacccgcc
    11461 gcgatggcgg caaacgggt
//
LOCUS       DAOSJM010000023        11256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5718_length_11256_cov_4.874029, whole genome shotgun sequence.
ACCESSION   DAOSJM010000023 DAOSJM010000000
VERSION     DAOSJM010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_11256_cov_4.874029"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PKX82_01640"
     CDS             <1..527
                     /locus_tag="PKX82_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF50977.1"
                     /translation="ELLPGFYYPAFRLLIIGVGLATALVLYLLVTRTRMGMQVRAGAS
                     NRDMALAMGTNVQRLFTAVFGLGAALCALAGGMLGPLLAVQVGMGESILILAFVVIVI
                     GGIGSIRGALVGALLVGLVDSGGRTLLPMLFEALFSPQVAADAAPAVASILIYVLMAT
                     VLAFKPRGLFPAHG"
     gene            520..1488
                     /locus_tag="PKX82_01645"
     CDS             520..1488
                     /locus_tag="PKX82_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF50978.1"
                     /translation="MARPEPSSVSLLQHKLNLRWTLPLLAALCALPWVAAALGDSFYV
                     TLATRILVFALAATSLNLILGFGGMVSFGHAAFVGVGAYTVGILMQHGITSAWLAWPL
                     AMAVSALLALLIGAISLRTQGVYFIMITLAFAQMLYYLAVSLKAYGGDDGLSLPGRSA
                     LGGGVELTDTTLYYVTLALLVATVVFITRLLNARFGQALQAIRENEVRMAALGYAVLR
                     YKLVAFALAGALAGLAGALLANQGGFVSPSLMQWSQSGMLMVMVILGGVGHVLGGLVG
                     AAAFLLMEEFLGHMTEHWQLGLGALLLLVVLVAPQGLMSLLKRRAP"
     gene            1485..2258
                     /locus_tag="PKX82_01650"
     CDS             1485..2258
                     /locus_tag="PKX82_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF50979.1"
                     /translation="MSTVPLLQVNDLVKRFGALAATDHANLHVQPGQIHALIGPNGAG
                     KTTLIQQISGALQPDAGHIVFDGHDVTTQPIHRRVHRGLSRSYQITNVFLRLSVLDNL
                     ALALQAVDGSSLRFWRAAHAEVQRMDRAAAVARRVGLGQRLDALAGALSHAEQRQLEV
                     GLALASGPKLLLLDEPLAGMGPDESQRMVELLHSLRADTTLLLVEHDMDAVFQLADAI
                     SVLVFGKVIASGAPAAIREDAEVRRAYLGDDAVPSPQSA"
     gene            2265..2975
                     /locus_tag="PKX82_01655"
     CDS             2265..2975
                     /locus_tag="PKX82_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF50980.1"
                     /translation="METLLDITGLDTAYGSSQVLFGLNLRIPAGGVATLLGRNGMGKT
                     TTVRSILGLTPARSGSIRFRGERIDQLSPDRIARMGVAVVPEGRQIFPNLTVLENLKA
                     FAAQRNASPTPWTLERIYALFPVLEQRTRNMGNQLSGGEQQMLAIGRALMTNPHLLIL
                     DEATEGLAPLIREDIWACLATLRAQGQSILVIDKYVQRLLPLAQQHTIIERGRVVWNG
                     DSATLAAQPDLWHQYVGI"
     gene            2991..3866
                     /locus_tag="PKX82_01660"
     CDS             2991..3866
                     /locus_tag="PKX82_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF50981.1"
                     /translation="MPKLYRDFDNQAQIDAQYNPSLALPDPAAPGRHFVAQSEKARAT
                     LPCVLDVPYGPTREETLDIFPAASRPDGSKAPVFIFIHGGYWRALSSKEFSCVALGLQ
                     PLGITSVVVNYALAPRVSIDEITRQTRAAAAWTLRNIQNYGGDPARVGIGGHSAGGHL
                     TAMCLQTPWAEDYGLPQDPFKAALLFSGLYDIEPLRYSYLQPQIQLDDGIVRRNSPAF
                     LTRPCATPAWVTWGSLESPEFARQAEIYDAAWRGHGNASELRAIDGADHFTVIRGLEQ
                     PDSEVAQWLAAKLVR"
     gene            3941..5110
                     /locus_tag="PKX82_01665"
     CDS             3941..5110
                     /locus_tag="PKX82_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HOF50982.1"
                     /translation="MDTLHPVAQEPYRDRKRYAWLFSLLVPSIVGIGPALVLATGDPR
                     MLWIQVVFFYLVLPVLDLLMGEDLSNPPESAVPALEADMYYRWVTYLLVPILWGSFIF
                     SAWFVATTPLPLHGLLAMVLITGSVGGFCINLGHEMGHKNTPLERWLAKLVLAPTGYG
                     HFSVEHNRGHHRDVATPIDPASSRMGESIYSFVLREMPGALVRAWNLEATRLRNAKLP
                     VWSLHNEILQPALITLLLWTALLVWLGVQILPFLVVASFWANFQLTSANYIEHYGLLR
                     AERAPGKYEPCQPHHSWNSNHIVSNWALFHLQRHSDHHAHPLRRYQSLRHFDNLPTLP
                     NGYFGMFLVAYIPPLWRYVMDERLLQVVGRDTSRINIDPAKRSAIVRRYQLTDAG"
     gene            complement(5170..8466)
                     /locus_tag="PKX82_01670"
     CDS             complement(5170..8466)
                     /locus_tag="PKX82_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOF50983.1"
                     /translation="MTDLSAEFKALANYRPTNKVRFVTAASLFDGHDAAINIMRRILQ
                     GMGAEVIHLGHNRSVDEVVTAALQEDVQGIAISSYQGGHVEYFKYMVDLLKSRGGEHI
                     QVFGGGGGVIVPPEIRELHAYGVSRIYSPEDGQRMGLQGMIGEMVMRCDKDLSALAPT
                     DLNAIQGHTEASWRALAQLLTAMEASKVNEALALAIRAQAATKNIAVVGITGTGGAGK
                     SSLTDELIRRLRLDQSDNLRVAVISIDPSRRKSGGALLGDRIRMNAISPWQQGPRVFM
                     RSLATRDFGSEISAALPDVIAACKVAGFDLIVVETSGIGQGDAAIVPHVDVPLYVMTP
                     EFGAASQLEKIDMLDFAEFVAINKFDRKGASDALRDVAKQVQRNKEAWTTPAEQMPVF
                     GTMAARFNDDGVTALYQAMLPRLQALGLPATDAGQLPRVNVRHSSNQTPVVPAARTRY
                     LAEISDTVRGYKKRARAQARLAREIQQLRAAAVMLEVDKPGKARAAEAALDLATHRVE
                     RQDADARKLLAQWPEMQKAYSGEEYVVKIRDKEIRTALTTQSLSGTTIRKVALPQYED
                     DGEVLKWLMLDNVPGSYPYTAGTFAFKRENEDPTRMFAGEGDAFRTNRRFKLLSSGMD
                     AKRLSTAFDSVTLYGNDPDPRPDIYGKVGNSGVSIATLDDLKVLYDGFDLCNPATSVS
                     MTINGPAPSILAMFMNAAIDQNIEKFKADNGRDPTDTEIAKIKEWVLANVRGTVQADI
                     LKEDQGQNTCIFSTEFSLKVMGDIASYFVHHNVRNFYSVSISGYHIAEAGANPISQLA
                     FTLSNGFTFVEAYLARGMHIDDFAPNLSFFFSNGMDPEYTVMGRVARRIWAVAMKEKY
                     GANERSQKLKYHIQTSGRSLHAQEIQFNDIRTTLQALIAIYDNCNSLHTNAFDEAITT
                     PTEDSVRRAMAIQLIINREWGLAKNENPSQGAFIIEELTELVEEAVLKEFEAIADRGG
                     VLGAMETGYQRGKIQDESMTYEMLKHTGELPIIGVNTFRNPHGDAVLDKLELARSTDD
                     EKQNQLKRLADFHARNKDKAPAMLKTLQQAVIENKNVFEVLMDAVRVCSLGQITTALF
                     EVGGQYRRNM"
     gene            complement(8626..10581)
                     /locus_tag="PKX82_01675"
     CDS             complement(8626..10581)
                     /locus_tag="PKX82_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOF50984.1"
                     /translation="MKKEVWFGLSIMAMVVIAAFVLLPSPSQMTNGHLGLLMLALVVV
                     AIMLGFPTAFTLMGMGMVFAWIAYRSQNPGLAVQQTLDLMVQRTYSVMSNDVLISIPL
                     FVFMGYLIERANLIEKLFKSMHLATARVPGSLAVATIITCAIFATATGIVGAVVTLMG
                     LLALPAMLRAGYSVQLSAGAITAGGCLGILIPPSVMLIVYGATAGVSVVKLYAGAFFP
                     GIMLASLYVLYVVVLAKWKPHLAPPMSEEDRRVPLPPFAEIVSTSISNKVLPGLIGAI
                     KGKRNAAVPMRELVNHLVIAVLPALTVALIMGAIYLKVTAPLPPETVEGVVAMGGGIS
                     DSAEDEAPGVGGLAEPEEAGGLQPLPAEAGTPESTAKAPVEEVQPAVAQAPAPAPAPA
                     PEAAVQERQPAPTSFWIGLVSCAVALALFYAYFSFARLEIFKMLLGSFFPLAMMILAV
                     LGTIVFGVATPTEAAAMGSLGGILLAAAYRRLNMQVMRESVYLTAKTSAMVCWLFVGS
                     SIFSAAFALLGGQEIINTWVLSMNMTPVQFMILAQVVIFLLGWPLEWTEIIVIFMPIF
                     IPLLPHFGIDPLFFGLLVALNLQTAFLSPPVAMAAFYLKGVSPPHVTLNQIFAGMLPF
                     MAIQVFAIFLLYQFPAIGMWLPEMLYK"
     gene            complement(10578..11204)
                     /locus_tag="PKX82_01680"
     CDS             complement(10578..11204)
                     /locus_tag="PKX82_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOF50985.1"
                     /translation="MQKLLLAVDKLSTVVGQAFSWLILALTLMISWEVFSRYAMDNPH
                     PWAFDVMSMMYGTLFMTAGAYTLSKNGHVRGDVLYGFFPPRLQAGLDLALYILFFIPG
                     VVAMAWAGYNFAAESWAINEHSNITANGPPVYPFKTVIPIAGAILLAQGLVEIVRCIV
                     CLKQGEWPSRGQDVEEVDVEKLKEMVHVKDEDIAKLDEYVIHPKGSHP"
BASE COUNT         2062 a         3529 c         3455 g         2210 t
ORIGIN      
        1 tggaactact gcccggcttt tattaccccg cttttcgcct gctcatcatt ggggtgggcc
       61 tggccacggc actcgtgctc tacctgctgg tcacgcgtac gcgcatgggc atgcaggtgc
      121 gggccggtgc ctccaaccgc gacatggcgc tggcaatggg caccaatgtg cagcgcctgt
      181 tcaccgccgt gtttggcttg ggtgctgcgc tgtgcgcgct ggccggcggc atgctggggc
      241 cgctgctggc ggtgcaggtg ggcatgggtg agagcatttt gattctggcc tttgtggtca
      301 tcgtgattgg tggcattggc tccatccgcg gcgcgctggt cggcgccctg ctggtgggcc
      361 tggtggactc cggcgggcgc accctgctgc cgatgctgtt cgaggcgctg ttcagcccgc
      421 aagtcgccgc cgatgcagcg ccggccgtgg cctccatcct gatttatgtg ctgatggcca
      481 cggtgctggc cttcaagccg cgtggactgt ttcccgccca tggctaggcc cgagccttca
      541 tccgtcagcc tgttgcagca caagttgaac ttgcgctgga cgctgccgct gctggccgcg
      601 ctgtgcgccc tgccctgggt ggcggccgcg ctgggcgaca gtttttatgt gacgctggcc
      661 acgcgcattc tggtgtttgc actggctgcc accagtctga atttgatcct gggttttggc
      721 ggcatggtga gttttgggca tgcggctttt gtgggcgtgg gcgcctacac cgtgggcatt
      781 ctcatgcagc atggcatcac cagcgcctgg ctggcctggc cactggccat ggcggtgagt
      841 gcgctgctgg cgctgctcat tggcgccatc agcctgcgca cgcagggcgt gtacttcatc
      901 atgatcacgc tggcctttgc gcagatgctg tattacctgg ccgtgtcgct caaggcctat
      961 ggtggcgacg atgggctctc gctgcccggc cgctcggcac tgggcggcgg ggtggagttg
     1021 accgacacca cgctgtatta cgtgacactc gccctgctgg tggccaccgt ggtctttatc
     1081 acgcgattgc tcaacgcacg ctttggccag gcactgcagg ccatccgcga aaacgaggtg
     1141 cgcatggccg cgctgggcta cgcggtgctg cgctacaagc tggtggcctt cgcgctggcg
     1201 ggcgcgctgg ccggcctggc aggcgcgcta ttggccaacc agggcggctt tgtcagcccc
     1261 tcgctcatgc agtggagcca gtccggcatg ctgatggtga tggtgattct gggcggcgtg
     1321 ggccatgtgc tgggtggcct ggtgggcgcc gcagcctttc tgctgatgga agaatttttg
     1381 ggccacatga ccgagcactg gcaactgggc ctgggcgcgc tgctcttgct ggtggtgctg
     1441 gtggcacccc agggactcat gagcctgctg aagcggaggg cgccatgagc acggtgccgc
     1501 tgctgcaggt caacgatttg gtgaagcgtt ttggcgcact cgctgccacc gaccacgcca
     1561 acttgcacgt gcagccgggc cagatccacg cactgatcgg ccccaatggc gcgggcaaga
     1621 ccacgctgat ccagcagatc tccggtgcgc tccaacccga tgccggccac attgtgtttg
     1681 acggccacga cgtcaccacc caacccatcc accgccgcgt gcaccgcggc ctgtcgcgca
     1741 gctaccagat caccaatgtg tttttgcgcc tgagcgtgct ggacaacctg gccctggcct
     1801 tgcaggccgt ggatggcagc agcctgcgct tctggcgtgc cgcgcatgcc gaggtgcagc
     1861 gcatggaccg cgccgctgcc gtcgcccgac gcgtcggttt gggccagcgg ctggatgccc
     1921 tggccggtgc gctttcgcat gccgagcaac gccagctgga agtgggcctg gcgctggcca
     1981 gtggccccaa attgctgttg ctggacgaac ccttggccgg catgggcccc gatgaatccc
     2041 agcgcatggt ggaactgctg cacagcctgc gcgcggacac cacgctattg ctggtggagc
     2101 acgacatgga cgcggtgttc cagttggccg atgcgatctc ggtgctggtg tttggcaaag
     2161 tgattgccag cggcgcacct gctgccattc gcgaagacgc cgaggtgcgc cgcgcctacc
     2221 tgggcgacga tgcagtgccc agcccccagt ctgcctaaaa cgccatggaa accctgctcg
     2281 acatcaccgg cctggacacc gcctatggca gcagccaggt gctgtttggc ctgaacctgc
     2341 gcatccccgc cggtggcgtg gccacactgc tgggtcgcaa cggcatgggc aagaccacca
     2401 cggtgcgcag catcctgggc ctcacacctg cgcgcagcgg cagcattcgt tttagggggg
     2461 agcgcatcga ccagctcagc cccgaccgca ttgcgcgcat gggcgtggct gtggtgcccg
     2521 aggggcggca gattttcccc aacctcacgg tgctggaaaa cctgaaggcc tttgccgcgc
     2581 agcgcaatgc cagccccact ccgtggacgc tggagcgcat ttacgcgctc ttcccggtgc
     2641 tggagcaacg tacccggaac atgggcaacc agctctcggg cggagaacaa cagatgctgg
     2701 ccattggccg tgcactgatg accaacccgc acctcttgat actggacgag gccaccgagg
     2761 gcctggcgcc gctgatccgc gaggacatct gggcctgcct ggccacgctg cgcgcgcagg
     2821 gccagagcat tctggtgatc gacaagtacg tgcaacgcct gttgccgctg gcgcagcagc
     2881 acaccatcat cgaacgcggc cgtgtggtct ggaacggtga ttccgccacg ctggctgcac
     2941 agccagacct ctggcaccaa tacgtcggca tctgaccgca ggaacacgat atgcccaagc
     3001 tttaccgcga cttcgacaac caggcgcaga ttgatgcgca gtacaacccc tcactggccc
     3061 tgccagaccc ggccgcaccg ggacggcact ttgtagcgca atccgagaaa gcgcgggcca
     3121 ctttgccctg cgtgctggat gtgccctacg gcccgacccg cgaagaaacg ctcgacatct
     3181 ttcccgcggc ttcgcggccc gatggctcca aggcgcctgt gttcatcttc atccatggcg
     3241 gctactggcg cgcactctcc agcaaggaat tcagctgcgt cgccctgggg ctgcagccgc
     3301 tgggcattac cagcgtggtc gtcaactacg cgctggcgcc gcgggtcagc atcgatgaaa
     3361 tcacccgcca gacccgtgcc gccgcggcct ggaccctgcg caatatccag aactacggtg
     3421 gcgacccggc acgtgtgggc attggtggcc actcggcggg cggccacctg acagccatgt
     3481 gcctgcagac cccatgggca gaagactatg ggctgccgca ggacccattc aaggctgccc
     3541 tgctgttcag cggcctgtac gacatcgagc cactgcggta cagctacctg caaccgcaga
     3601 tccagctgga cgatggcatc gtgcgccgca actcgcccgc ctttctgacg cgcccctgcg
     3661 ccacaccggc ctgggtgacc tggggttcgc tggaatcgcc ggaatttgct cgccaggcgg
     3721 agatttatga cgccgcgtgg cgcgggcacg gcaatgcgtc cgagctgcgt gccatcgacg
     3781 gcgccgacca cttcacggtg atccgtgggc tggaacaacc cgacagcgaa gtggcgcagt
     3841 ggctggcggc caagctggtg cgctaggggt tcaaaagcta cgtcacctcg gggaaacacc
     3901 ccctgttgcc agccggggtt tgggcgcaga atgcctcgcc atggacaccc tacaccccgt
     3961 cgcgcaggaa ccctaccgtg atcgcaagcg gtacgcatgg ctgttctctt tgctggtgcc
     4021 cagtatcgtg ggcatcgggc ccgcgctggt gctggccacc ggggacccgc gcatgctgtg
     4081 gatccaggtg gtgttttttt acctggtgct cccggtgctg gacctgctca tgggcgagga
     4141 cctgagcaac ccgcccgagt cggctgtccc ggcgctggag gcggacatgt actaccgctg
     4201 ggtcacctac ctgctggtgc ccattctgtg gggctccttc attttcagtg cctggtttgt
     4261 ggctaccacg cccctgccgc tgcacggctt gctggccatg gtgctgatca ccggctcggt
     4321 tggtggcttc tgcatcaacc tggggcacga gatgggccac aagaacacgc cgctggagcg
     4381 ctggctggcc aagctggtgc tggcgcccac cggttacggg cacttctcgg tcgaacacaa
     4441 ccgcggccac caccgcgatg tggcaacgcc gatcgacccg gcttcctcac gcatgggtga
     4501 gtcgatttac agctttgtgc tgcgcgagat gccaggggcg ctggtgcggg cctggaacct
     4561 ggaagccacc cgcctgcgca atgccaagtt gcccgtctgg tccctgcaca acgaaatact
     4621 gcagccggcg ctgatcacgc tgctgctctg gaccgcattg ctggtatggc tgggcgtgca
     4681 gatactgccg tttctggtgg ttgcgtcgtt ctgggcgaac ttccagctga catcggccaa
     4741 ctacatagag cactacggtt tgctgcgggc agagcgggca ccgggcaaat acgagccctg
     4801 ccagccccac cactcctgga acagcaacca catcgtgtcg aattgggcgc tgttccattt
     4861 gcagcgccat tcagaccacc atgcccatcc tctgcggcgc taccagtcgc tgcgccactt
     4921 tgacaacctg cccacgctgc ccaacggcta cttcggcatg ttcctggtgg cctacattcc
     4981 accgctgtgg cgctacgtga tggacgagcg cctgctccaa gtggtgggcc gcgataccag
     5041 tcgcatcaac atcgatccgg caaaacgctc cgccatagtc cggcgttacc agctcacgga
     5101 tgccggataa attgctctgt ttttaatagc tgcccacact ggatatacaa gggccagagg
     5161 ccaatttgac tacatattgc gtcggtactg gccgcccacc tcgaacaggg cagtggtgat
     5221 ttgccccagt gagcacaccc gcactgcatc catcagcact tcaaacacgt ttttgttttc
     5281 aatcacggcc tgctgcaggg tcttaagcat ggccggagcc ttgtctttat tccgggcgtg
     5341 gaagtcggcc aggcgcttga gctggttttg cttttcgtcg tccgtcgagc gggccagttc
     5401 cagcttgtcc agcacggcat cgccatgcgg attacggaag gtgttgacgc cgatgatggg
     5461 cagctcgccc gtgtgcttga gcatttcgta ggtcatgctc tcgtcctgga tcttgccgcg
     5521 ctggtagccg gtttccatgg cgcccagcac accgccccgg tcagcaatgg cctcaaattc
     5581 cttgagtacc gcctcttcca ccaactcggt gagttcttcg atgatgaagg cgccctgact
     5641 ggggttctca ttcttggcca ggccccactc gcggttgatg atgagctgga tggccatggc
     5701 gcggcgcacc gagtcctcgg tgggcgtggt gatggcctcg tcgaacgcgt tggtgtgcag
     5761 gctgttgcag ttgtcgtaga tcgcaatcaa ggcctgcagc gtggtgcgga tgtcgttaaa
     5821 ctggatttcc tgcgcatgca gtgagcggcc gctggtctgg atgtggtatt tcagcttctg
     5881 gctgcgctcg ttggcaccgt acttctcttt catcgccacc gcccagatgc ggcgcgccac
     5941 gcggcccatg acggtgtact cggggtccat gccgttgctg aagaagaagc tcaggttggg
     6001 cgcaaagtcg tcaatgtgca tgccgcgcgc caggtaggcc tcgacaaacg tgaagccatt
     6061 gctcagcgtg aacgccagct ggctgatcgg gttggcgcca gcttcggcaa tgtggtaacc
     6121 gctgatggac acgctgtaga agttgcgcac gttgtggtgc acaaagtagc tggcaatgtc
     6181 gcccatcacc ttgagcgaga actcggtgga gaagatgcag gtgttctggc cctggtcttc
     6241 tttcaggatg tcggcctgca ccgtgccgcg cacattggcc agcacccatt ccttgatctt
     6301 ggcaatttcg gtgtcggtgg ggtcgcggcc attgtcagcc ttgaacttct cgatgttctg
     6361 gtcgatggcc gcattcatga acatggccag aatgctgggt gccggaccat tgatggtcat
     6421 ggacacgctg gtggccgggt tgcacaggtc gaacccgtcg tacagcacct tcaggtcgtc
     6481 cagcgtggct atggagacgc ccgaattacc taccttgccg tagatgtccg gacgcgggtc
     6541 ggggtcgttg ccatacagcg tgaccgagtc gaacgcggtg gacaggcgct tggcatccat
     6601 gccactggag agcagcttga agcggcggtt ggtgcggaag gcatcgccct cgccggcaaa
     6661 catgcgggtg gggtcttcgt tctcgcgctt gaaggcaaag gtgcctgcgg tgtaggggta
     6721 gctgccgggc acgttgtcca gcatcagcca cttcaaaact tcgccatcgt cttcgtactg
     6781 gggcagggcc accttgcgga tggtggtgcc actcaaggat tgggtggtga gcgcggtacg
     6841 gatttccttg tcgcggatct tcaccacgta ctcttcaccg ctgtaggcct tttgcatttc
     6901 gggccactgg gccaacagtt tgcgggcatc ggcatcctga cgctccacac gatgggtggc
     6961 caggtccagc gctgcttcgg ccgcacgcgc cttgccgggt ttgtctactt ccagcatcac
     7021 ggccgcggca cgcaactgct gaatttcgcg ggccagccgc gcctgcgcac gcgcacgctt
     7081 cttgtagccg cgcacggtat cgctgatctc ggccagatag cgcgtgcggg ctgcaggcac
     7141 taccggggtc tggttgctgc tgtggcgcac gttgacgcgc ggcagctgac cggcgtctgt
     7201 ggccggcagc cccagcgcct gcaggcgcgg cagcatggcc tggtacaggg cagtcacacc
     7261 gtcgtcattg aagcgcgcgg ccatggtgcc gaagaccggc atctgctcgg cgggtgtggt
     7321 ccaggcttcc ttgttgcgct gcacctgctt ggccacatcc cgcagcgcat cgctggcgcc
     7381 cttgcggtcg aacttgttga tggccacgaa ctcggcaaag tcgagcatgt cgatcttttc
     7441 cagctggctg gctgcgccaa actccggtgt catcacatac aacggcacat ccacgtgtgg
     7501 aacgatggcc gcatcgccct ggccaatgcc cgaggtttcc accacgatca gatcgaaccc
     7561 cgccaccttg caggcagcaa tcacatcggg cagcgcggcg ctgatttcgg agccgaagtc
     7621 ccgggtggcc aggctgcgca tgaatacgcg gggaccctgc tgccaggggc tgatggcgtt
     7681 catgcggata cggtcgccca atagtgcgcc accgctcttg cggcggctgg ggtcaatgga
     7741 gatgactgcc acgcgcaggt tgtcgctctg gtccaggcgc aggcggcgga tcaactcgtc
     7801 ggtcagcgat gacttgccag caccaccggt tccggtgatc cccacaaccg ctatattttt
     7861 tgtagctgct tgcgcacgaa tggcgagggc tagagcctca tttaccttgg aagcctccat
     7921 ggcggtgagc aactgggcca aagcacgcca gctggcttct gtatggccct gtatggcatt
     7981 caggtcggta ggggccaggg cgctcagatc tttgtcgcag cgcatgacca tttcgccgat
     8041 catgccctgc aggcccatgc gctggccatc ctcagggctg taaatgcgac tgacaccgta
     8101 agcgtggagt tcacgaattt ccggcggcac gatcacacca ccaccaccac cgaatacctg
     8161 gatgtgctcg ccaccccggc tcttgagcag gtccaccatg tacttgaagt actccacgtg
     8221 cccgccctgg taggaactga tggcgatgcc ctgcacgtct tcctgcaaag cagcggtaac
     8281 cacttcgtcc acactgcggt tgtgcccgag gtggatgact tctgcgccca tgccctgcag
     8341 gatgcggcgc atgatgttga tagcggcgtc gtgcccatcg aacaggctcg cagcagtgac
     8401 aaagcgcacc ttgttcgtgg ggcggtagtt ggccagggcc ttgaattcag cggacaggtc
     8461 agtcattttg gaatgtctcc gggagtcagc aggcaatgag aatgcatgtg cctgacttga
     8521 cgtttacgta aacgtcaatc ttatctagtt ttccctggaa aagacacggg gcgccagagc
     8581 gccccgatat ccagaacctg gcacgcatgt gcctggcacc atgttttact tgtaaagcat
     8641 ctccggcagc cacatgccaa tggccgggaa ctggtacagc aggaagatcg caaacacctg
     8701 gatggccata aagggcagca tgccggcaaa aatctggttc agcgtcacat gcggggggct
     8761 cacacccttc agataaaacg cagccattgc cacaggcggg ctcaggaacg cggtctgcaa
     8821 attcagcgcc accagcaagc caaaaaacag gggatcgatg ccgaagtggg gcagcaacgg
     8881 aatgaagatc ggcatgaaaa tcacgatgat ctcggtccac tccagcggcc agcccagcag
     8941 gaaaatcacc acctgcgcca ggatcatgaa ctgcactggc gtcatgttca tcgacagcac
     9001 ccaggtgttg atgatttcct gcccgcccag caacgcgaac gccgccgaga agatgctcga
     9061 tcccacaaac aaccagcaca ccatggcact ggtttttgcc gtgaggtaga ccgactcccg
     9121 catcacctgc atattcagcc ggcgatacgc tgcggccagc aagatgcccc ccagcgagcc
     9181 catggctgcc gcctcagttg gcgttgccac gccaaaaacg atggtgccca gtaccgccag
     9241 aatcatcatc gccagcggga agaatgaccc cagcagcatt ttgaagattt ccaggcgtgc
     9301 aaaactgaaa tacgcataga acaacgccag cgccacagca cagctgacca gaccaatcca
     9361 gaagctcgtg ggtgcgggct gacgctcctg cactgcagcc tcaggggcag gggcaggggc
     9421 aggagcaggt gcttgcgcca ctgcgggttg gacttcctcc actggggctt tagcggtgga
     9481 ttcgggagtg cccgcctcag ccggcagtgg ctgcaagccc cccgcttctt cgggctctgc
     9541 cagtccgccc acgcctgggg cttcgtcttc cgcagaatca gagatgcctc cacccatggc
     9601 gaccaccccc tccaccgttt ctggtggcaa gggcgcagtg accttcagat agatcgctcc
     9661 catgatgagc gccaccgtca gcgcgggcag taccgcaatc accagatggt tgaccagttc
     9721 gcgcatcgga acggccgcat tgcgcttgcc tttaatggca ccaatcaagc caggcaatac
     9781 cttgttgctg atactggtgg acacaatctc agcaaaaggt ggcaggggca cgcgacggtc
     9841 ctcttcggac atggggggcg ccaggtgcgg cttccatttg gcaagcacca cgacgtacaa
     9901 cacgtacaga ctggccagca tgatgccggg aaagaatgct cccgcgtaga gcttgaccac
     9961 ggagacgcca gccgtagcac cataaacaat caacatgacg gacggcggaa tcagaatgcc
    10021 caggcatcca ccagccgtaa tggccccggc agagagttgc acgctatagc ccgcacgcag
    10081 catggcgggc agagccagca agcccatcag cgtgaccacg gcgccgacaa tgcccgtggc
    10141 agtcgcgaag atcgcacagg taatgatggt tgccacggca agtgagcccg gcacgcgcgc
    10201 agtggccagg tgcatgcttt tgaacagctt ttcgatcaga ttggcgcgct cgatcaggta
    10261 gcccatgaac acgaacagcg gaatggatat cagcacgtcg ttggacatca ccgagtaggt
    10321 gcgctgcacc atcaaatcca gcgtttgctg gaccgccagc cccgggtttt gacttcggta
    10381 ggcaatccag gcgaacacca tccccatgcc catcagggta aaggccgttg gaaagcccag
    10441 catgatggcc accaccacca aggccagcat cagcaagccc aaatggccat tcgtcatttg
    10501 ggaaggtgat ggcagcaata caaaagccgc aatcacgacc atggccatga tcgacaggcc
    10561 aaaccagact tcttttttca tggatggctt cccttggggt gaatgacgta ctcgtcgagc
    10621 ttcgcaatgt cttcgtcttt cacgtggacc atttctttca gtttttcaac gtcaacttcc
    10681 tcaacgtcct ggccgcgcga gggccactca ccctgcttca ggcaaacgat gcatcggaca
    10741 atttcaacca gcccttgcgc cagaagaatg gcgccagcaa tcggaatcac ggtcttgaac
    10801 gggtagaccg ggggaccatt ggcggtgatg ttggaatgct cattgatcgc ccaggactcc
    10861 gctgcaaagt tgtatccggc ccacgccatg gcgaccacac caggaataaa aaacaggatg
    10921 tacaaagcca ggtccaatcc ggcctgcaaa cgcggcggga aaaagccata cagcacatct
    10981 ccgcgcacat ggccgttctt ggacagggtg taagcaccgg ccgtcatgaa cagggtgccg
    11041 tacatcatgc tcatcacgtc aaatgcccac ggatgggggt tgtccatcgc atagcgcgag
    11101 aacacctccc aggaaatcat cagcgtgagt gccagtatca gccaggagaa cgcctgacct
    11161 accacggtac tgagcttgtc aaccgcaagc aatagttttt gcatattcaa accgcctaag
    11221 taaatcagcc atccaggcaa cccccggatg gctgaa
//
LOCUS       DAOSJM010000024        11185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5765_length_11185_cov_4.201438, whole genome shotgun sequence.
ACCESSION   DAOSJM010000024 DAOSJM010000000
VERSION     DAOSJM010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11185
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_11185_cov_4.201438"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1716
                     /locus_tag="PKX82_01685"
     CDS             <1..1716
                     /locus_tag="PKX82_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOF50986.1"
                     /translation="GSAEDDMFDEDLPQPKGSALSREAIEERRQSLYESMAHKLAVAS
                     WDGDRDTLADQPDPRDWSAVAAERHTCTARHCPRYKECSYYNARTQLAEANVIVANHD
                     LVLASLGMKTLPDLDNCLVVFDEGHHLPAVALDQFGSSMDLSNLRWLDKLPKTMNEVA
                     GKLALHTGEDVQTVASQLKAALTSLARMAMDLVWAQTGKNARGEGNDGTVRFAHGVLP
                     EPLLEPVTQIHAQASGLSKVLEALGVDVKAVAKDDPSQAVLCAQLYAQLGGLAPRLAS
                     LVSTSQLLLEHGEQPLAKWLKVESESGFLTVTAYACPIVPGDLLRQFLWSQVRGAIVT
                     SASLTSCGSFDYFLKEAGLSNNPHVTALEVDSPFDYARQGSMTVVITHADPKNAEAYT
                     AEMVQALMNDIEQVQRGALVLFTSRAQMRAATEALPGHLLDMVLVQGTQSRTRLLAAH
                     AARVESGMPSVLFGLQSFGEGLDLPGALCETVFIAKLPFAPPSDPVDEARAEWLKRVG
                     RDPFNELVIPATGVKLLQWTGRAIRTEDDHARVICYDKRLVQTAYGRRMLSGLPAYRL
                     DQRNN"
     gene            1784..2497
                     /locus_tag="PKX82_01690"
     CDS             1784..2497
                     /locus_tag="PKX82_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOF50987.1"
                     /translation="MGAQWKAKGKELVANARGKLFTKLVKEIMVAARSGADPAGNARL
                     RLAVEQARKASMPKDTLDRAIKKGAGLTGEAVNYEVAMYEGYAPHRVAVMVECLTDNV
                     NRTAPEMRVLFRKGQLGTSGSVAWDFDHLGMIEAEPATPGADPEMAAIEAGAQDLEPT
                     DENGVTMFLTDPADLDLVSRALPAQGFTVLSAKLGYKPKNPIDPSTLSAEQMEEVEAF
                     LAAIDANDDVQNVYVGLAG"
     gene            complement(2494..3564)
                     /gene="rsmH"
                     /locus_tag="PKX82_01695"
     CDS             complement(2494..3564)
                     /gene="rsmH"
                     /locus_tag="PKX82_01695"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOF50988.1"
                     /translation="MTDASPPHKRRVRYSGTHPRQFAEKYKELDPARHAEEVDKVMQR
                     GQTPAGMHRSICVDEILAILNPQPGETGLDATLGFGGHTQTLVPRVLPGGHMFGMDVD
                     PIELPRTTARLRALGFDEAALSTHRRNFSEMASLLPQAGGGFDFVLADLGVSSMQIDN
                     PARGFSFRADGPLDLRLDPTAGQSASELLQSVTRQHLRDLLIDHSDEPFAIPLAAALQ
                     GQYVETTTQLAELVRAAMQGALKASMPQEERLVETKKALQRTFQALRIEVNNEFGVLD
                     TLLAALPACLKPGGRVAILSFHSGEDRRVKKAFQTGERDGTYSSVAPDPIRASMEERR
                     ANSRSTSAKLRWAVRAGGPLAA"
     gene            3605..6277
                     /locus_tag="PKX82_01700"
     CDS             3605..6277
                     /locus_tag="PKX82_01700"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOF50989.1"
                     /translation="MQTSPPENPAWHQREVQDLAQEHLVDPALGLHEAEALLRSERHG
                     ANELQTRKKRGLMVLLLDQFKDFIVLVLLAAAVISGVIGDLVDTMAILVIVVLNGLMG
                     FLQTWRADRAMAALQAMTAAQATVLRGGQVQTVPARTLVPGDIVLLEAGNHVPADLRL
                     IEIAQLQVDESALTGESLSVAKSTAALPDAAESALGDRINMAFKGTTATQGRARGLVV
                     AVGMDTELGKVAQLLDGEDRTTPLQRRLAAFGKRLALAVIGICAIIFLVGIVRGEAPL
                     LMALTAISLAVAAIPEALPAVVTVLLALGARRMVAVHALVRRLPSVETLGSVTTICSD
                     KTGTLTQNRMHAELLLAAGAPWVPGDTLPGPVHREALRAAVLCNDAHRHAQGTGTEWQ
                     GDPTETALVLAALSANLDKTALEAATPRVQEQPFDSGRMRMTTFHRDGGAYIAYTKGA
                     PESVLPCCDAQWTPHGRQPIDHTALVGQAQALAAQGLRVLALARRDHRVLPDTNRLVG
                     VERGLEFLGLIALIDPPRPEAARAVQECITAGITPVMITGDHPATARAIAFRLGIIPD
                     AQAEVLTGQELAELNDIALQSRVDQVRVYARVDPAQKIRIVEALQARGHTVAMTGDGV
                     NDAPALKRADIGVAMGKGGTDVAREASSMVLLDDNFATIVAAVREGRRIYDNIRKFVR
                     YAMTGNSGEIWTLFLAPMLLLPIPLQPIHILWVNLVTDGLPGLALAAEPAERDVMQRP
                     PRAPAESLFANGMWQHILGIGLLIGGLCLAVQAWALSTGHAHWQTMVFTVLTLSQMVH
                     ILAIRSERESLWQMPLGSNKPLLGAVALTVMLQLATIYVPALNPIFKTEPLSLDELAI
                     CVAAALTVGIAVEAEKAWRRRTGRKPASAADSPA"
     gene            complement(6284..7288)
                     /locus_tag="PKX82_01705"
     CDS             complement(6284..7288)
                     /locus_tag="PKX82_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOF50990.1"
                     /translation="MSHYDLYAIGNALVDSEYEVSDAQLQAMGVEKRHMTLIDAERRA
                     ALLAAVHGMHARRTGGGSAGNTVVALAQLGGKGFYSCRVADDELGAFYREDLVANGVA
                     TNLTHTKPPEGQTGICMVMVTPDAERSMSTFLGATAELDALSLHAKDIARSKIYYMEG
                     YLAASPTGLDAALQGRAIAKEAGVALAVTLSDMSMINFCRPGLDAMVGDGLDYLFCNE
                     EEAQTWCGSQDLATITATVSKLARVVCLTRSAKGCLVLEGGQQTEVPAAQVKAVDTNG
                     AGDMFAGAFLYAVTQGHSHAQAAWLGNQAAGRVVSQYGNRLNAAAMADIQKAFTHYLG
                     "
     gene            complement(7311..8084)
                     /locus_tag="PKX82_01710"
     CDS             complement(7311..8084)
                     /locus_tag="PKX82_01710"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HOF50991.1"
                     /translation="MSAFLEETVTEVHHWTDRLFSFKTTRDQALRFSNGHFTMIGLRV
                     DGKPLLRAYSIVSANYEDHLEFLSIKVQDGPLTSRLQHIQVGDKIIVGKKPTGTLLID
                     YLLPAKNLYLIGTGTGLAPFMCLIRDPATYEKFEKVILVHGVREVKELAYTDYIQHEL
                     PNHEFLGEMISAQLLYYPTVTREPFRNQGRVTTLLETNKIAADLGLPNLDPANDRIMI
                     CGSPALNKDMRELLDARGFVEGSTTTPGDYVVERAFVEQ"
     gene            8224..8601
                     /locus_tag="PKX82_01715"
     CDS             8224..8601
                     /locus_tag="PKX82_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOF50992.1"
                     /translation="MSLVPEFTPSSREPGLLVACLCAQWCRTCDDYQPLFTQLQTEYP
                     QARFEWVDIEDEADLVDPVEVENFPTILIAVRGQARFFGTVTPHIDTLRRLVQAQLTA
                     DGTGKQSAEIQALAERLQTHTTG"
     gene            complement(8635..9168)
                     /locus_tag="PKX82_01720"
     CDS             complement(8635..9168)
                     /locus_tag="PKX82_01720"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOF50993.1"
                     /translation="MKHFFLLLALVCAMALPAMAAESSASPGYEVKPWPAGQKAPELR
                     ATALDGKVWALKDLRGRAVLVNFWASWCEPCRAEMPSLQALAQLEGDGRLVVLAVNHK
                     ESAATIARYVQTTQLSLPVLPDPQGELARQWGISVYPSTVLMGADGRVRSVVRGELDW
                     TGEAARKLIGPLLPPSR"
     gene            complement(9242..>11185)
                     /gene="alaS"
                     /locus_tag="PKX82_01725"
     CDS             complement(9242..>11185)
                     /gene="alaS"
                     /locus_tag="PKX82_01725"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOF50994.1"
                     /translation="LAAILQHVHSNYEIDLFDALIKAASRETGCTDLNNKSLRVIADH
                     IRATAFLVSDGVIPSNEGRGYVQRRIVRRAIRHGYKLGQKTPFFHKLVKDLVQVMGAA
                     YPNLASQEARITEVLRVEEERFFETLEVGMSILDVEIARINEVTTSPDGPAGDLPPNA
                     RTISGEIAFKLHDTYGFPLDLTNDVCRENGVEVDEAGFKAAMEAQKAKGRAAGKFKMD
                     KALEYTGAGNTFVGYEQLTQATKIVALYADGTSVATLKAGQSGVVVVESTPFYSESGG
                     QVGDQGAIFCDGGLFQVEDTQKIKADVFGHHGVLKQGILAVGDAVTAHVDAAVRASTV
                     RNHSATHLMHKALREVLGEHVQQKGSLVNAERTRFDFAHNAPVTDEQIRQIETIVNAE
                     VLANAAAQARVMDIESAQKTGAMMLFGEKYGETVRVLDIGSSRELCGGTHVKATGDIG
                     LFKVVGESGVAAGVRRIEAVTGANALHYLQDLEDTVGAAAATLKAPVAELGNRLHQMV
                     DQVKALEKEISALKGKLASSQGDELMNSAVEVNGVKVLAAKLEGADAKTLRDTMDKLK
                     DKLKTAVIVLGAVDGDKVQIAAGVTNDTTGKVKAGELANFVAGQVGGKGGGKADMAMA
                     GGTEPAKLAGALASVQAWVTAKL"
BASE COUNT         2056 a         3572 c         3531 g         2026 t
ORIGIN      
        1 ggcagcgcag aagacgacat gttcgacgaa gacctgccgc aacccaaggg ttcggccctg
       61 tcccgggaag ccatcgaaga acgccgccag tcgctttacg agagcatggc gcacaagctg
      121 gccgtggcca gctgggatgg tgaccgcgac accctggctg accagcctga cccgcgcgac
      181 tggtctgcgg tggcggccga gcgccacacc tgcaccgcac gccactgccc gcgctacaag
      241 gaatgcagct actacaacgc gcgcacccaa ctggccgagg ccaacgtgat tgtggccaac
      301 cacgacctgg tgctggcctc gctgggcatg aaaaccctgc ccgacctgga caactgcctg
      361 gtcgtgtttg acgaaggcca ccacctgccc gccgtggcac tggaccagtt tggcagcagc
      421 atggacctgt ccaacctgcg ctggctggat aagttgccca agaccatgaa cgaggtggcc
      481 ggaaaactgg cgctgcacac cggtgaggac gtgcagaccg ttgccagcca gctcaaggct
      541 gcgctaacca gtctggcgcg catggccatg gacctggtgt gggcacaaac cggtaaaaac
      601 gcgcgcggcg agggcaacga cggcaccgtg cggttcgccc acggcgtcct gcctgagccg
      661 ctgctggaac cggtgacgca gatccacgca caggcctcgg gcctgtccaa agttctggaa
      721 gcgctgggag tggatgtcaa agccgtggcc aaggacgatc cgtcccaggc agtgctgtgc
      781 gcccagctgt acgcgcagct tggcggtctg gcgccgcgcc tggccagcct ggtctccaca
      841 tcgcaattgc tgctggagca cggagagcag cctctggcca agtggctgaa ggtggagagc
      901 gaaagcggtt tcctcacggt gaccgcatac gcctgcccca tcgtgccggg cgacctgctg
      961 cgccagttcc tgtggagcca ggtgcgcggc gccatcgtca ccagtgcctc gctcaccagc
     1021 tgcggcagct ttgactattt cctgaaagaa gcgggcctgt caaacaaccc ccatgtcacc
     1081 gcactggaag tggacagccc cttcgactat gcccgccagg gaagcatgac cgtggtgatc
     1141 acgcatgccg accccaagaa tgcagaggcc tataccgccg agatggtgca ggcactcatg
     1201 aacgacatcg agcaggtaca gcgcggcgcg ctggtgctgt tcacttcgcg cgcccagatg
     1261 cgtgccgcca ccgaggccct gcccggccac ctgctggaca tggtgctggt gcagggcacc
     1321 cagtcccgca cacgcctgct ggcggcccac gccgcgcggg tggagagcgg catgccatcg
     1381 gtgctgtttg gcctgcagtc gttcggcgaa ggtctggacc tgccgggggc cttgtgcgaa
     1441 accgtgttca ttgccaagct gccctttgcc ccgcccagtg acccggtgga cgaagcgcgg
     1501 gccgagtggc tcaagcgtgt ggggcgcgat ccgttcaacg aactcgtcat cccggccacc
     1561 ggcgtgaagc tgctgcaatg gaccggacgg gcgattcgca ccgaagacga ccatgcgcgt
     1621 gtcatttgct acgacaagcg gctggtacag accgcttatg gacgcaggat gctgtcggga
     1681 ttgcctgcat accgactgga tcaaagaaac aactgacagt aggtgtataa acctgccaga
     1741 ctttggcgtc cgatgggcgc cgcaacttga aggaaactca gacatgggcg cgcagtggaa
     1801 agcaaaaggt aaggaactgg tagccaacgc caggggcaaa ctgtttacca aactcgtcaa
     1861 ggaaatcatg gtggcggccc gtagcggcgc agaccctgcc ggcaacgccc ggctgcgcct
     1921 ggccgtggaa caggcccgca aggcgtcgat gcccaaggac acactggacc gcgccatcaa
     1981 gaaaggcgcc ggactcacgg gtgaggcggt gaattacgaa gtggcgatgt acgaaggtta
     2041 tgcaccgcac cgcgtggcgg tcatggtcga atgcctgacc gacaacgtca accgcaccgc
     2101 gcccgaaatg cgggtgctgt tccgcaaggg ccagctggga acatccggtt ccgtggcctg
     2161 ggatttcgac cacctgggca tgattgaagc cgaaccagcc acccccggag ccgacccgga
     2221 aatggccgcc atcgaagccg gtgcacagga cctggagcct accgatgaaa acggggtcac
     2281 catgttcctg actgaccccg ccgacctcga cctggtcagc cgtgccctgc ccgcgcaggg
     2341 atttaccgtg ctgtccgcca aactgggtta caagcccaag aaccccatcg accccagcac
     2401 gctgagcgcc gagcagatgg aagaagtgga agccttcctc gcagccatcg atgccaacga
     2461 cgacgtgcag aacgtctacg ttggcctggc cggctaagct gccagcggcc cgccggccct
     2521 gacggcccag cgcagcttgg cagaggtgga ccgggagttg gcccggcgct cttccatgga
     2581 ggcgcgaatg gggtcggggg ccacactgga gtaggtcccg tcgcgctcgc cagtctggaa
     2641 tgcctttttc acacggcggt cttcacccga atgaaagctc aggatcgcca cgcgccctcc
     2701 gggcttgagg caggccggca gggccgccag cagcgtatcg agcaccccga actcattgtt
     2761 gacttcgatg cgcagtgcct gaaaagtgcg ttgcagcgct tttttggttt ccaccagacg
     2821 ctcctcctgc ggcatgctcg ctttgagcgc gccctgcatg gcggcgcgca ccagttcggc
     2881 cagttgcgtg gtggtttcaa cgtattggcc ctgcaaagcg gccgccagcg gaatggcaaa
     2941 gggctcatcc gagtggtcga tcagcaggtc gcgcagatgc tggcgggtga cgctctgcaa
     3001 cagttcgctg gcagattgac ccgcggtagg gtccaggcgc aggtccagcg gaccgtccgc
     3061 acggaagctg aaaccccgcg ccggattgtc aatctgcatc gatgacaccc ccagatcggc
     3121 cagcacgaag tcaaagccgc caccggcttg cggcagcagg ctggccattt cgctgaagtt
     3181 gcggcggtgc gtgctcagcg cagcctcatc aaagcccagc gcgcgcaggc gtgccgtggt
     3241 gcggggcaat tcaatcgggt ccacatccat gccaaacatg tggccaccgg gcagcacgcg
     3301 cggcaccagg gtctgggtat ggccaccgaa ccccagcgtg gcatccaagc cggtttcccc
     3361 tggctgggga ttgagaatgg ccaggatttc gtccacgcag atggagcggt gcatgccagc
     3421 gggggtctgg ccccgctgca tgaccttgtc gacttcctcc gcatgccgtg cggggtcgag
     3481 ttccttgtat ttctctgcaa actgacgcgg atgggtgccg gagtagcgca cacggcgctt
     3541 gtggggtggg gatgcgtcgg tcattggcca ttatcagccc aagcccttgc gctacagtag
     3601 gtcgatgcag accagccccc ctgagaaccc cgcctggcat caacgtgagg tgcaggacct
     3661 ggcacaggag cacctggtcg acccggcgct ggggctgcac gaagcggagg cactgctgcg
     3721 cagcgagcgc catggcgcca acgagctgca gacccgcaag aagcgcggcc tcatggtttt
     3781 gctgctggac cagttcaagg atttcattgt gctggtgctg ctggctgcag ccgtcatttc
     3841 cggcgtgatc ggtgacttgg tggataccat ggcgattctg gtaatcgtgg tgctcaacgg
     3901 gctgatgggc ttcctccaga cctggcgcgc agaccgcgcc atggcagcgc tccaggccat
     3961 gacggcggca caggccacgg tgctgcgcgg tggacaggtt cagaccgtgc ccgcacgcac
     4021 cctggtgccc ggtgacattg tcctgctgga agcaggaaac catgttcctg cagatttacg
     4081 cctgattgaa attgcacagt tgcaggtcga cgagtctgcc ctgacgggcg aatccttgag
     4141 tgtggccaag agcaccgcgg cactgccaga cgccgccgaa agcgctctgg gcgacagaat
     4201 caatatggcg ttcaagggga ccaccgccac gcagggccgt gcgcgcggtc tggtcgtggc
     4261 agtcggcatg gataccgagt tgggcaaggt ggcccaactg cttgacgggg aagaccgcac
     4321 cacgccactg cagcgccgtc ttgccgcatt cggcaagcgc ctggcactgg cggtgattgg
     4381 catctgtgcg attatttttc tggtcggcat tgtgcgtggg gaagccccgt tgctgatggc
     4441 actgacagct atcagcctgg ccgtggcggc cattcccgaa gcgctgccgg cagtcgtcac
     4501 cgtactgctg gcgctgggcg cccggcgcat ggtggcggtc catgcgctgg tacggcggct
     4561 gccatcggtc gagacactgg gttcggtcac caccatctgt tccgacaaaa ccggcaccct
     4621 cacccagaac aggatgcatg ccgagctttt gctcgccgca ggagcaccgt gggtgccggg
     4681 agacaccctt cccggaccgg tacaccgcga ggcgctgcgc gccgcagtgc tctgcaatga
     4741 cgcccaccgc cacgcccagg gaacaggcac cgagtggcaa ggcgacccga ccgagaccgc
     4801 gctggtactt gccgcactaa gcgccaacct ggacaaaaca gcactggaag cggccacccc
     4861 ccgcgtgcag gagcaaccct ttgactccgg gcgcatgcgc atgaccacct tccaccgcga
     4921 tggaggtgcc tatatcgcct ataccaaggg ggcgccggaa tccgtgctgc cgtgctgcga
     4981 cgcccaatgg acgccacacg gacgccaacc gatagaccac accgccctgg tgggccaggc
     5041 gcaggctctg gcagcgcagg gcttgcgggt actggccctg gcgcggcgcg accacagggt
     5101 gctacccgac accaataggc tggtgggcgt ggaacgcgga ctggagttcc tgggactgat
     5161 tgcgctgatc gacccgccac gccccgaggc ggcacgcgca gtgcaggaat gcatcacggc
     5221 aggcatcacc ccggtgatga tcacgggcga ccatccggct actgcacgcg ccattgcgtt
     5281 ccgcctgggg atcatccccg acgcgcaggc tgaagtgctc accgggcagg agctggcgga
     5341 actgaacgat atcgccctgc aatcacgggt tgaccaggtc agggtgtatg cgcgggtgga
     5401 tccggcacaa aaaatccgca ttgtcgaagc actgcaggca cgtggccaca ccgttgccat
     5461 gactggcgat ggggtcaatg acgcacctgc actgaagcgg gccgacattg gtgtggccat
     5521 gggcaaaggc ggcaccgatg tcgcgcgaga ggcctccagc atggtgctgc tggacgacaa
     5581 cttcgccacc atcgtggcgg cggtgcggga aggtcggcgt atttacgaca acatccgcaa
     5641 gtttgtgcgc tatgcgatga ccggcaattc gggcgagatc tggaccctgt tcctcgcacc
     5701 catgctgttg ctccccatcc ccctgcaacc gatccacatc ctgtgggtca acctggtgac
     5761 cgacggcctg cccgggttgg ccctggccgc cgaacccgcc gaacgcgatg tgatgcagcg
     5821 gccaccacgg gcaccggccg aaagcctgtt tgccaatggc atgtggcaac acattctggg
     5881 cattggcctc ctgattggcg gactgtgcct ggcggtgcaa gcctgggcgc tatcgaccgg
     5941 gcacgcgcat tggcaaacca tggtctttac cgtgctgacg ctgagccaga tggtgcacat
     6001 cctggcgatt cggtccgagc gcgaatctct ctggcagatg cccctgggga gcaacaagcc
     6061 actgctgggc gctgtggcgc tgacggtcat gctgcagctg gcgacgatct atgtgccggc
     6121 actgaacccc attttcaaaa ccgaaccgct cagcctggat gagctggcca tctgtgtggc
     6181 cgcggcactc accgtcggca tcgccgtgga ggcagaaaaa gcctggcggc gacgcacggg
     6241 ccgcaagccc gcttccgccg cagacagccc ggcgtgagcc agactagccc aggtagtggg
     6301 tgaaggcctt ctggatgtct gccatggccg cagcgttgag gcggttgccg tactgcgaca
     6361 ccacacgacc cgccgcctgg ttacccagcc aggccgcctg ggcatggctg tggccctggg
     6421 tgacggcgta caggaaggcg cccgcaaaca tgtcgccggc gccgttggta tcgacagcct
     6481 tgacctgggc tgcgggcact tcggtctgct ggccaccttc aagcacgaga cagcccttgg
     6541 cactgcgggt caggcacacc acgcgcgcca gtttggacac ggtagcggtg atggtggcca
     6601 gatcctggct gccgcaccaa gtctgcgctt cttcctcatt gcagaacagg tagtccagcc
     6661 catcgcccac catcgcatca aggccgggtc ggcagaagtt gatcatgctc atgtcgctga
     6721 gcgtgacggc cagcgccaca cccgcttcct tggcgatggc acgcccttgc agcgcggcat
     6781 cgagtcccgt gggcgatgcg gccaggtagc cttccatgta gtagatcttg gagcgagcaa
     6841 tgtccttggc atgcagcgac aacgcatcca gttccgccgt agcacccaga aacgtgctca
     6901 tgctgcgctc ggcatcgggc gtgaccatga ccatgcagat gccggtctgg ccctcgggtg
     6961 gcttggtgtg cgtgaggttg gtcgccacgc cattggccac gaggtcttca cggtaaaacg
     7021 cgcccagctc gtcatcggcc acgcggcagg agtaaaaccc cttgccaccc agttgcgcca
     7081 gtgccaccac ggtgttgcct gcggagccac cgccggtgcg gcgtgcgtgc atgccatgca
     7141 ctgctgccag caatgcggcg cggcgctcgg catcgatcag cgtcatgtgg cgcttttcga
     7201 cgcccatggc ctgcaactgt gcgtcgctca cttcgtattc ggaatccacc agggcattgc
     7261 caatggcgta gaggtcgtaa tgggacatgg atggttttct tctggctggc ttattgttcg
     7321 acgaaagcgc gttcgaccac gtagtcgccc ggtgtggtgg tgctgccttc gacaaagccg
     7381 cgcgcatcca gcagttcacg catgtccttg ttcaacgccg ggctgccgca gatcatgatg
     7441 cggtcgttgg cggggtccag attgggcagg cccagatcgg cagcaatctt gttggtttcc
     7501 agcagcgtgg tgacacggcc ctggttgcgg aagggttcgc gcgtcacggt ggggtagtac
     7561 agcagctgtg ctgaaatcat ctcgcccagg aactcgtggt tgggcagctc atgctggatg
     7621 tagtcggtgt aggccagttc tttcacctcg cgcacaccgt gcaccaggat gactttttcg
     7681 aacttctcgt aggtagccgg gtcccggata aggcacatga agggggccag gccagtgccg
     7741 gtgccgatca ggtagaggtt tttggcgggt agcaggtaat cgatgagcag ggtgccggtg
     7801 ggctttttgc cgacgatgat cttgtctccg acctggatat gctgcagacg cgaggtcagc
     7861 gggccgtcct gcaccttgat gctcaggaac tccagatggt cttcgtaatt ggcgctgacg
     7921 atgctgtagg cgcgcagcag gggcttgccg tccacgcgca ggccgatcat ggtgaagtgg
     7981 ccgttggaga agcgcaaggc ctggtcccgc gtggtcttga agctgaatag gcgatcggtc
     8041 cagtggtgga cttcggtgac ggtttcttcc aggaatgcac tcatgaaggg ccttgcaata
     8101 gttcgggtgg tggctcgggg gaaaccctag agtgtaaaac ctccggcccc ctaccccgtg
     8161 acgaagatca agagaccatc gcccacggca cagtttcccg gcagtttctg cgatcatcgg
     8221 agcatgtctc tggtacccga attcacccca tcctcccgcg agcccgggct gctggttgcc
     8281 tgcctgtgcg cccagtggtg ccgcacctgc gatgactacc agcccctgtt tacccagttg
     8341 cagaccgaat atccgcaggc gcgcttcgag tgggtcgata ttgaagacga agccgatctg
     8401 gtcgatccgg ttgaagtcga gaatttcccg accatcctga ttgcggtccg tggccaggca
     8461 cgtttctttg ggacggtgac gccccacatc gataccttgc gccgcctcgt tcaggcgcaa
     8521 ttgacggcgg atggaaccgg caagcagagc gccgaaatcc aggcactggc agaacgcctg
     8581 cagacacaca ccaccgggta gtaccccgca ccccttgaat gccccggcaa tgccctagcg
     8641 ggatgggggc aacagggggc cgatcagctt gcgcgcggcc tcccccgtcc agtccagctc
     8701 gccgcgcacc acactgcgca cccgaccatc ggcacccatc agcaccgtcg atgggtagac
     8761 cgaaatgccc cactggcgtg ccagctcacc ctgcggatcg ggcagcaccg gcaggctgag
     8821 ttgcgtggtt tgcacatagc gcgcgatggt ggcggcagat tccttgtggt tcaccgccag
     8881 aacgaccagc cggccgtccc cttcgagttg tgccagggcc tgcagcgaag gcatttcggc
     8941 acggcagggc tcgcaccagc tggcccagaa gttgaccagc accgcccgcc cgcgcaagtc
     9001 tttcaacgcc cagaccttgc cgtccagtgc ggtcgcgcgc agctcgggtg ccttctgacc
     9061 cgcaggccag ggcttgacct catagccggg agacgcagac gactctgcag ccatggcagg
     9121 cagggccatg gcacagacaa gcgccaaaag gaggaaaaag tgtttcatcc ggggattaca
     9181 agcaaaatag gcctctagcc cccgtcgaat atgcgcaacc agctacaaaa actgtagcag
     9241 attacagttt ggcagtcacc caggcctgca cgctggccag cgcgccggcc agcttggcag
     9301 gctcggtacc accggccatg gccatatcgg ccttgccgcc gcccttgcca ccgacctggc
     9361 ccgccacaaa gttggccagt tcaccggcct tgaccttgcc cgtggtgtcg ttggtcacgc
     9421 ccgcagcaat ctgcaccttg tcgccatcca ccgcgcccag cacgataacg gcggtcttga
     9481 gcttgtcctt gagcttgtcc atggtgtcgc gcagggtctt ggcgtcggcg ccttccagtt
     9541 tggccgccag tactttcacg ccgttcacct ccacggcgct gttcatcagc tcgtcgccct
     9601 ggctggaggc cagcttgccc ttgagggcgg aaatttcttt ttccagcgcc ttgacctgat
     9661 cgaccatctg gtgcaaccgg ttgcccaact cggccacagg ggccttgagc gtagcagcgg
     9721 cagcgcccac ggtgtcttcc aggtcctgca aatagtgcag cgcgttggca ccggtcacag
     9781 cttcaatgcg tcgcactcca gcggccacac cgctctcacc caccaccttg aacaagccaa
     9841 tgtcgccggt cgccttgacg tgcgtaccac cgcagagttc acggctggag ccgatgtcga
     9901 gcacgcgcac ggtctcgccg tacttctcgc cgaacagcat catcgcgccg gtcttctggg
     9961 ccgactcgat gtccatcacg cgcgcctggg cagcggcgtt ggccagcact tcagcgttga
    10021 cgatggtttc aatctggcga atctgctcat cggtcaccgg ggcgttgtgt gcaaagtcga
    10081 agcgggtgcg ctcggcattt accagcgatc ccttttgctg cacatgctcg cccagcactt
    10141 cgcgcagcgc tttgtgcatc aggtgggtgg cggagtggtt gcgcacggtg gaggcgcgca
    10201 ctgcagcgtc cacatgggcc gtcacggcgt cgcccacggc cagaatgccc tgcttgagca
    10261 cgccgtggtg gccaaataca tcggccttga tcttctgcgt gtcttccacc tggaacaggc
    10321 caccatcgca gaagatcgcg ccctggtcac cgacctgacc gccgctctcg gaatagaacg
    10381 gggtgctttc caccaccacg acgccggatt gaccggcttt gagcgtcgca accgacgtac
    10441 cgtctgcgta gagcgctacg attttggtag cttgtgtgag ctgctcgtag cccacgaaag
    10501 tgttgcctgc gccggtgtac tccagcgcct tgtccatctt gaatttgccg gctgcgcggc
    10561 ccttggcctt ttgcgcttcc atggcggctt taaaacctgc ttcgtccact tctacaccat
    10621 tttcgcgaca gacgtcgttg gtcagatcga gtggaaaacc gtaggtgtcg tgcaacttaa
    10681 aggcaatttc acctgaaatc gtccgtgcat ttgggggcaa gtcacctgca gggccatctg
    10741 gagaagtggt cacctcgttg atacgtgcaa tttccacatc aaggatgctc atcccaactt
    10801 ccagcgtttc aaagaaccgc tcctcttcca cacgcagcac ttcggtgatg cgcgcttcct
    10861 gcgatgccag gttgggatac gctgcgccca tcacctgcac caggtccttc accagcttgt
    10921 ggaaaaacgg ggtcttctgg cccagcttgt agccgtggcg gatcgcgcgg cgcacgatgc
    10981 ggcgctgcac gtagccgcga ccttcgttgc tcgggatcac gccatcgctc accaggaagg
    11041 cggtggcgcg gatgtggtca gcgatcacac gcaagctctt gttgttcagg tcggtacaac
    11101 cggtttcgcg ggatgcggcc ttgatcagcg cgtcgaacag gtcgatctcg tagttgctgt
    11161 gcacgtgctg caggatggcg gccag
//
LOCUS       DAOSJM010000025        11175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5768_length_11175_cov_4.567176, whole genome shotgun sequence.
ACCESSION   DAOSJM010000025 DAOSJM010000000
VERSION     DAOSJM010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5768_length_11175_cov_4.567176"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="PKX82_01730"
     CDS             complement(<1..1357)
                     /locus_tag="PKX82_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOF50995.1"
                     /translation="MTQTSLTANHHCVALVGNPNCGKTALFNLLTGARQKVANYAGVT
                     VERKVGTARLANGKTFSVVDLPGSYSLTPATPDEQVMLEVIEGRREGEAAPDAIVAVV
                     DATNLRMNLRLVLELKRLGKPLIVALNMADVARAQGLAIDVAGLSRELGCPVVETVAV
                     RHEGHAQLMTQLQTQLESIAVASPAATVSAAAQSSAELQREVRRILALVAPRAAKVGR
                     FQHRLDAVVMHPVGGLVVLAAVLFLIFQAVFSWANLPMDVIKGTMAAVGEWTLASMPE
                     GPLRSLLVDGVIAGVGGVLVFLPQILILFFFILLLEDSGYLPRAAFLLDNLMGKVGLS
                     GRAFIPLLSSFACAIPGIMATRTIANWKDRLATIMVAPLMTCSARLPVYALLIGAFVP
                     ERDVGWFDLRGLTLFGLYVAGVVGAMAVAWVFKRVWMKTGYQPLMLELPPYRVPSLRN
                     LG"
     gene            complement(1364..1627)
                     /locus_tag="PKX82_01735"
     CDS             complement(1364..1627)
                     /locus_tag="PKX82_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOF50996.1"
                     /translation="MSMLLSQLPNGAHALVERVESASAEVGESTVRRLGELGFIPGEP
                     IQMLRRGPGGREPLAVQVGETLFALRLVEAQCIHVQAAAAAGV"
     gene            complement(1679..5245)
                     /gene="dnaE"
                     /locus_tag="PKX82_01740"
     CDS             complement(1679..5245)
                     /gene="dnaE"
                     /locus_tag="PKX82_01740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOF50997.1"
                     /translation="MFIHLRLHTEFSVVDSTCRIDEVVKAAAKDGQPALGITDLNNLF
                     GAIKFYKEARGKGVKPVIGAEVVLEGLGADAAATSRIVLLVQNKQGYLNLSELIARAY
                     TQNVVKAQAVVKMAWLKELNEGLIALSGAQAGPVGQALVQGDQTRAHEAALQLASTFP
                     HRFYMELQRAGRTDDESHVVAAVQLAARIRLPVVATHPIQFTEPDDYEAHEARVCIAD
                     GEILSNPKRVRRFTREQYFKSAAQMEALFKDIPSAIANTLEIAKRCSLTLVLGKPQLP
                     DFPTPEVNGQRMSPEDYFRYASHEGLKERMAHLYPDPVEREKQMPRYLERLEFELGTI
                     LKMGFPGYFLIVGDFINWAKNNGCPVGPGRGSGAGSLVAYALKITDLDPLQYNLLFER
                     FLNPERVSMPDFDIDFCQSNRDRVIDYVKLKYGKEAVSQIATFGTMAARGVIRDVGRV
                     LDMPYMFCDGISKLIPNKPGTNVTLQLPPTDGKKNDKYIYASEAEPILAEREAKEEDV
                     KTLLEMARKLEGMTRNIGMHAGGVLIAPGKLTDFCPLYQQPGSESAVSQYDKDDVEAI
                     GLVKFDFLGLATLTILEIAREFIMKRHPGQENFAYENLPLDDANVYRLFSEGKTESVF
                     QFESVGMQRMLVDAKPSRLEDLIALNALYRPGPMDLIPSFVARKHGKEVIEYPHPLVA
                     DMLSETYGIMVYQEQVMQTAQILGGYSLGGADMLRRAMGKKKAEEMAEHRAIFRAGAA
                     KNDISEQKADEVFDLMEKFAGYGFNKSHAAAYSLLAYYTAWIKVHYTAEFFCANMTVE
                     MDDTDKLKVLFEDAEKMGLSFEPPNINRGFYRFEPISNKEIRYGLGAIKGTGQQAIEA
                     IVAAREGRGPTEESGPFKSLFDFARRVDRSRLNKRCVEALIKAGAFDTLQLNRAALVE
                     SIDRAFDFAAASAANANQHGLFDMGGEDDHGSSTQEPDLVDALPWGVREQLTQEKSAV
                     GFYLSGHLFDEMALEVRRFAKRPIEEMLETREPQVLAGIISGFKVINGNRGKVGIFTL
                     DDKSAAIEATADETLLTTYRSLLQDDTLVIVSGRLQPGRNGFAARFVVQQVWSLEQAR
                     CRFGKYLRVAVSGKMPDVARLVREFPAQREMSEQGELVRGLSVRLQIQRMVDAASQEA
                     AGCAGAQAELHLGEEARFYPSDAALAEWRAHADQGKAVIAYD"
     gene            5368..5790
                     /locus_tag="PKX82_01745"
     CDS             5368..5790
                     /locus_tag="PKX82_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF50998.1"
                     /translation="MRNSIFKPHGAVQIEVSGSIVTAHMRGHWNIEMRNQAAQAMGEP
                     VQSLNAAGPWGIIHVLHDTLIYSEEIFSTSRQDYATRPAASRLVAVAFVIDPQVEGAQ
                     LLLYRFENLLDGIIPSGVFADLPSAQSWVQQMIAGAGH"
     gene            5800..6555
                     /locus_tag="PKX82_01750"
     CDS             5800..6555
                     /locus_tag="PKX82_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOF50999.1"
                     /translation="MNTVLNISSYKFVPLPDAPALRETLLARALRRDLKGTILLAEEG
                     INLFLAGPAEDVRGFITELQTDPRFADITPKESWSDTRPFRKMLVKVKGEIIRMNYPT
                     IRPAEGRAPAVAPATVKRWLDQGHDDEGRPVVTLDTRNDFEVDAGTFEGAIDWRITKF
                     TEFPQAVLDHKAELEGKTVVSFCTGGIRCEKAAILMQEMGLDHVVQLEGGILKYFEET
                     DGSHYRGSCFVFDERRAVDADLAANPGAVSSPS"
     gene            complement(6643..7188)
                     /locus_tag="PKX82_01755"
     CDS             complement(6643..7188)
                     /locus_tag="PKX82_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOF51000.1"
                     /translation="MAKVAVIFHSGYGHTQRMAQSVAQGAGGELITIDADGNVPESAW
                     DTLAAADAIIFGSPTYMGSVSWQFKKFADASSKPWYTQAWKDKIFGGFTASANVNGDK
                     HSTLHYFMTLAMQHGGIWAGTGLNYNNTKASQRSDVNYLASSAGPMAQTPGDAGGDEM
                     NAGDLETARLFGARIAALASK"
     gene            complement(7220..7921)
                     /locus_tag="PKX82_01760"
     CDS             complement(7220..7921)
                     /locus_tag="PKX82_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOF51001.1"
                     /translation="MLTLRTSQERGYADHGWLKSFHSFSFAGYYDPKHMGWGNLRVIN
                     EDRIAPGTGFGTHGHRDMEIVSYVISGNLAHKDSMGNVKGIPPGDVQRMSAGSGVQHS
                     EFNHAPNDTTHFLQIWIEPNVKGIDPSYEQKTFADTEKQGVLRLVASPDAAQGSVKIH
                     ADARMYSGLFDGDQTATLELGARRKGYVHLVRGVLEVNGTVLQSGDAALLHEESRITL
                     ANGKDAEVLVFDLQD"
     gene            8036..8977
                     /locus_tag="PKX82_01765"
     CDS             8036..8977
                     /locus_tag="PKX82_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF51002.1"
                     /translation="MQNARDFLTPDALSMLQAIAKAGSFAAAAREMGMVPSALTYRVR
                     QMEDALDVLLFDRSSRQARLTEAGSELLREGTRLLTEIDAVANRVKRVATGWESQFTI
                     AVDTIISRSTVMELCDAFYQLAPPTRIRLRDETLSGTLEALTSGQADLAIGAGPDSAT
                     AADLHSKPLGNVTFVYAVAPHHPLAHAPEPLSDAVIQKHRAVAVADSVQRGGGQTFGL
                     LAGQDVFTVASMQAKLDAQLRGMGGGNLPLCMANAFIDTGRLVVKKTERGQRQVSVHY
                     AWRGGKSNTSGRALQWWLQQLESPATRAALLENHRSM"
     gene            9040..10083
                     /locus_tag="PKX82_01770"
     CDS             9040..10083
                     /locus_tag="PKX82_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51003.1"
                     /translation="MTTSRHIAIVGAGIAAITCARTLVQAGHRVTVFEKSHSVGGRMS
                     TRSSPFGTFDHGAQYFTVRDPRFELALQTTPGLCRPWSANTVRVLDSHGRVAAAGLPA
                     REPHWVPKPGMNALVRQWAQPLADAGRIELQTKVTAIEPDSLNAKQWQLRTQGEGDSS
                     HVFGGFDAVVLAIPNVQASALLQSSPKGAALAKKISRVTVAPCLTLMLAFPQAVQPGL
                     TTLGPQWSAARSTHHRIAWLARESSKPGRGAVERWTVQASAAWSQEHLHDDVDRIEAK
                     LIKAFSEVTGIRAEPAHADVHRWMYAKTELALGESHLWDGKAGIGVCGDWCLGHRVED
                     AFVSGLELALKIV"
     gene            10165..11136
                     /gene="gluQRS"
                     /locus_tag="PKX82_01775"
     CDS             10165..11136
                     /gene="gluQRS"
                     /locus_tag="PKX82_01775"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HOF51004.1"
                     /translation="MHSTRNSSTYRGRFAPSPTGLLHAGSLVAALASWLDARAYGGKW
                     LVRIEDVDTPRCVPGTDQLILQQLAACGLQPDEPPVWQSQRTALYQRALDQLVARGMA
                     YACACSRKDIEAALAQSGHSKPRNGELVYPGTCREGLHGKPARAWRFRTSNLVSNQAP
                     APVEYAQVATENIASSKSEVWWTDRRLGLQQQDVERDVGDFVLLRADGLFAYQLAVVV
                     DDAQQGITHVVRGEDLADNTARQILLQRALHLPTPQYLHTPLVLGPNGEKLSKQNGAS
                     PVDTSTPDAAFAALCQAAAALDLLPAAGTPADALALWTRQWGDRYSL"
BASE COUNT         2121 a         3670 c         3318 g         2066 t
ORIGIN      
        1 ggcccaggtt gcgcaaactg ggtacgcggt acggtggcaa ctccagcatc agtggctggt
       61 agccggtttt catccagacg cgcttgaaca cccaggccac cgccatggcg cccaccacgc
      121 ctgccacata caggccaaac agggtcaggc cccgcaggtc aaaccagccc acatcacgct
      181 ccggcacaaa ggccccaatc aacaaggcat agacgggcag gcgcgccgag caggtcatca
      241 gcggcgccac catgatggtg gccaggcggt ccttccagtt ggcgatggtg cgcgtggcca
      301 tgatgccggg aatggcacag gcaaagctgg aaagcagtgg aatgaaggcc cgcccggaca
      361 agcccacctt gcccatcagg ttgtccagca ggaaggccgc acgcggcagg tagccggaat
      421 cttccagcag caggatgaag aaaaacagaa tcaggatctg cggcagaaac accagtacgc
      481 cgcccacacc ggcaatcacg ccatccacca acaggctacg cagcgggcct tccggcatgc
      541 tcgccagcgt ccactcgccc accgccgcca tggtgccctt gatcacgtcc atcggcaggt
      601 tggcccagct gaacaccgcc tggaaaatca ggaatagcac cgctgccagc accaccagcc
      661 cccctaccgg gtgcatcacc acagcgtcca gccggtgctg gaagcgaccc actttcgccg
      721 cgcgcggcgc caccagggcc agaatgcggc gcacttcgcg ctgcagctcg gctgagcttt
      781 gcgcagctgc ggacaccgtc gcggcgggag aagctaccgc aatactttcc agttgggtct
      841 gcagctgcgt catcaactgg gcatgccctt cgtgcctgac cgccacggtt tccaccaccg
      901 ggcaacccag ctcacgcgac agtccggcca cgtcaatggc cagcccctgc gcgcgggcca
      961 catcggccat attcagcgca acgatcagcg gtttgcccag gcgcttgagt tcgagcacca
     1021 agcgcagatt catgcgcaag ttggtggcgt ccaccaccgc cacgatggcg tcgggcgccg
     1081 cttcgccttc cctgcggcct tcaatcacct ccagcatcac ctgctcatcg ggcgtcgctg
     1141 gcgtcaggct gtacgaaccc ggcaagtcca ccaccgaaaa ggtcttgcca ttggccagac
     1201 gcgcggtccc cactttgcgt tccaccgtga caccggcgta attggcaacc ttctggcgcg
     1261 cgccggtgag cagattgaag agcgcggtct tgccgcagtt ggggttgccg accaatgcaa
     1321 cacaatggtg attggccgtc agggatgttt gcgtcatggt gcttcagact ccggcggcag
     1381 cggcagcctg cacatggatg cactgggctt ccaccagacg cagcgcgaac agtgtttccc
     1441 ctacctgcac cgccagcggc tcgcggccac ctgggccgcg gcgcaacatc tggataggct
     1501 caccgggaat aaaccccagc tcccccagac gccgcacagt ggactcaccg acctcggcag
     1561 acgccgactc cacgcgctcg accagcgcgt gggctccgtt gggtaattgt gaaagtagca
     1621 tggacatgcc ccattctaaa tgagagttca tctcatttag attgatgtag gtcatacatc
     1681 aatcgtaggc aatgaccgcc ttgccctggt ccgcgtgggc acgccactcg gccagcgcgg
     1741 catcgctggg gtaaaaacgc gcctcttcgc ccagatgcaa ctccgcttgg gcaccggcac
     1801 aacctgccgc ctcctgcgag gcggcatcca ccatgcgctg tatctgcaag cgcaccgaaa
     1861 ggccgcgcac cagctcgccc tgctccgaca tttcccgctg ggccggaaac tcccgcacca
     1921 ggcgcgccac atcgggcatc ttgccgctga ctgccacccg caaatacttg ccaaagcggc
     1981 agcgcgcctg ctccaggctc cagacctgtt gcaccacaaa ccgcgcggca aagccgttgc
     2041 gcccgggttg caggcggcca ctgacgatga ccagcgtgtc gtcctgcaac aggctgcggt
     2101 aggtggtcag cagcgtttcg tcggctgtcg cttcgatcgc agccgatttg tcgtccagcg
     2161 tgaagatgcc gactttgccg cggttgccgt tgatgacctt gaagccactg atgatgccgg
     2221 ccagcacctg cggctcgcgg gtctccagca tctcctcgat gggccgcttg gcaaaacggc
     2281 gcacctccag ggccatctcg tcaaacaggt gacccgagag gtaaaagccc accgcagatt
     2341 tttcctgcgt cagctgctcc cgcacgcccc agggcaacgc atccaccagg tccggctctt
     2401 gcgtgctgga gccatggtcg tcctcgccac ccatgtcaaa caggccatgc tggttggcat
     2461 tggctgcaga agccgccgca aaatcaaacg cacggtcgat gctttccacc agtgcggcgc
     2521 ggttgagttg cagcgtgtca aaggcgcctg ccttgatcag tgcttccaca caacgcttgt
     2581 tcaggcggct gcgatcgaca cgacgcgcga agtcaaacag acttttgaat ggaccgctct
     2641 cttcggtggg gccacggcct tcccgcgcgg ccacgatggc ttcaatcgcc tgctggccgg
     2701 tacccttgat ggcgcctagc ccgtagcgga tttccttgtt ggagatcggc tcaaagcggt
     2761 agaacccgcg gttgatgttg ggtggctcaa acgagaggcc catcttttcc gcgtcttcaa
     2821 acaacacctt caacttgtcg gtgtcgtcca tttccaccgt catgttggcg cagaagaact
     2881 cggcggtgta atgcaccttg atccacgcgg tgtagtacgc caagagtgag taggcagccg
     2941 cgtgcgactt gttgaagcca tagcccgcaa acttctccat caagtcaaaa acctcgtcgg
     3001 ctttttgctc gctgatgtcg ttcttggcag cgcctgcgcg aaaaatggcg cggtgctcgg
     3061 ccatttcttc ggccttcttc ttgcccatgg cgcggcgcag catgtcggcg ccaccgagcg
     3121 agtagccgcc cagaatctgc gcggtctgca tcacctgctc ctggtagacc atgatgccgt
     3181 aggtctcact cagcatgtcg gccaccagcg ggtgcgggta ctcgatcacc tctttgccat
     3241 gtttacgcgc cacaaagctg ggtatcaggt ccatggggcc tggacggtac aaggcgttca
     3301 gcgcaatcag gtcttccagg cgcgagggtt tggcgtccac cagcatgcgc tgcatgccca
     3361 cgctttcaaa ctggaacacg gactcggtct tgccctcact gaacaatcga tacacattgg
     3421 catcgtccag cggcaggttt tcgtaggcaa agttctcctg gcccggatgg cgcttcatga
     3481 tgaactcgcg cgcaatctcc agaatcgtca gcgtggccag acccaaaaag tcgaacttca
     3541 ccaggccaat ggcctccacg tcatccttgt cgtactggct cacggccgac tcgctgccgg
     3601 gctgctggta caacgggcaa aaatcggtca gcttgcccgg cgcaatcagc acgcccccgg
     3661 cgtgcatgcc gatattgcgg gtcatgccct ccaacttgcg cgccatctcc agcagggttt
     3721 tgacgtcctc ttccttcgct tcgcgctcgg ccagaatcgg ctccgcctcg ctggcgtaga
     3781 tgtacttgtc gttcttcttg ccgtcggtgg gcggcagctg cagcgtgacg ttggtgccgg
     3841 gtttgttggg gatgagcttg ctgatgccat cacaaaacat gtagggcatg tccagcacac
     3901 ggcccacgtc gcggatcacg ccgcgcgcgg ccatggtgcc gaaggtggca atctggctga
     3961 ccgcctcctt gccatacttc agcttcacgt agtcaatcac ccggtcgcga ttgctctggc
     4021 aaaagtcgat atcgaagtcg ggcatcgaca cccgctcggg gttcaggaac cgctcgaaca
     4081 gcaggttgta ctgcagcgga tccaggtcgg taatcttgag cgcgtaggcc accagactgc
     4141 cagcacccga gccccggccc ggccccaccg ggcatccgtt gttcttggcc cagttgatga
     4201 agtcgcccac gatcaggaag tagcccggaa agcccatttt cagaatggtc cccagctcaa
     4261 actccagacg ctccaggtag cgtggcatct gcttttcgcg ctcaaccggg tccgggtaca
     4321 ggtgcgccat gcgctccttg agtccctcat gcgatgcgta gcggaagtag tcttccggcg
     4381 acatgcgttg gccgttgact tccggcgtcg gaaaatccgg caactgcggt ttgcccagca
     4441 ccagggtcag gctgcagcgc ttggcaatct ccagtgtgtt ggcaatcgca gacggaatgt
     4501 ccttgaacag cgcctccatt tgcgccgcgc tcttgaaata ttgttcgcgg gtaaagcggc
     4561 gcacccgctt ggggttactg aggatttcac catcggcaat acagacgcgg gcctcgtggg
     4621 cctcgtagtc gtcgggctcg gtaaactgga tcgggtgcgt tgccaccacc ggcagccgga
     4681 tgcgggcggc cagctgcacg gcggccacca catgggactc gtcatccgtg cgcccggccc
     4741 gctgcaactc catgtagaaa cggtgtggga acgtgctggc caattgcagt gccgcttcgt
     4801 gcgcacgggt ctggtcaccc tggaccagtg cctgaccgac cggcccggcc tgcgcacccg
     4861 acagggcaat cagcccttcg ttcaactcct tgagccaggc catcttgacc accgcctggg
     4921 ccttgaccac attctgggtg taggcgcggg caatcagttc ggacaggttg aggtagccct
     4981 gcttgttctg caccagcagc acaatgcgcg aggtcgcggc agcgtctgcg cccaggcctt
     5041 ccagaaccac ctccgcacca atcaccggct tgacaccttt accccgtgct tctttgtaaa
     5101 acttgatcgc accaaacagg ttgttgaggt ccgtgatgcc cagggccggc tgcccgtcct
     5161 tggccgccgc cttgacaacc tcgtcaatgc ggcaggttga atcaacaacg gaaaattcgg
     5221 tgtgcaggcg caggtggata aacatgcgtg cattgtagtt ttgccaacca caaagccact
     5281 tccgggcggc acaagaaatt acgtcacaat cagcagtagt acgggtggca cgtgggagcc
     5341 ggtggctgca agcaacaaaa gttttgtatg agaaattcga ttttcaaacc ccatggggcg
     5401 gtgcagattg aggtgtccgg gtccatcgtg acggcacaca tgcgcggaca ctggaacatc
     5461 gagatgcgca accaggccgc acaggccatg ggcgagcccg tgcagtcact caacgcagca
     5521 ggcccctggg gcatcatcca cgtactgcac gacaccctga tttacagcga agaaattttt
     5581 tcgacctctc gccaggacta cgccacgcgc ccagcagcct cacggctggt agccgtcgct
     5641 tttgtcatcg atccgcaggt cgagggcgcc cagttgttgc tctaccggtt cgagaacctg
     5701 ctcgatggca tcatcccttc cggcgtcttt gccgacctgc cctccgccca gagctgggtc
     5761 caacagatga ttgcgggtgc cggccactaa aatcagacgg tgaacaccgt actgaacatc
     5821 tctagctaca aattcgtccc gcttccggac gcgcccgcat tgcgggaaac cctgctggcg
     5881 cgcgcactgc gccgtgacct caagggcacc atcctgctgg cagaagaagg tatcaaccta
     5941 tttctggccg ggcccgcaga ggatgtgcgc ggattcatca ccgaactgca gaccgatccc
     6001 cgctttgccg acataacgcc caaagagagc tggtccgaca cgcggccgtt tcgcaaaatg
     6061 ctggtcaagg tcaagggcga aatcatccgc atgaactacc ccaccatccg ccccgcggag
     6121 ggccgtgcgc ctgcggtggc acctgccacg gtcaagcgct ggctggacca gggacacgac
     6181 gacgaagggc ggccagtcgt tacgctggat acgcgcaacg actttgaagt ggatgccggc
     6241 accttcgaag gcgccatcga ctggcgcatc accaaattca ccgaattccc gcaagcggtg
     6301 ctcgaccaca aggccgagct ggaaggcaaa accgtggtga gcttctgcac tggcggcatc
     6361 cgctgcgaaa aggctgccat cctgatgcag gaaatggggc tggaccatgt ggtccagctt
     6421 gaaggcggca tcctcaagta ctttgaggaa accgacggca gccactaccg cggcagctgc
     6481 tttgtgtttg acgagcgccg ggccgtggac gccgatctgg ctgccaatcc tggggcagtc
     6541 agcagtccgt cctaagtccg gagcaaaaaa aggagcgcat tgcgctcctt tttttatagc
     6601 tagccgcgct tattccgcgg ggtctagcgg ccattttacc tattatttgg aagccagagc
     6661 ggcaatccgg gcgccaaaca ggcgtgcggt ttccaggtca cccgcattca tttcatcgcc
     6721 accggcgtcg ccgggcgtct gggccatggg gcctgcgctg gaagcgaggt agttgacgtc
     6781 gctgcgttgc gaggccttgg tgttgttgta gttcaggccc gtgccggccc agatgccgcc
     6841 gtgctgcatg gccagggtca tgaagtaatg cagggtggag tgcttgtcgc cattcacgtt
     6901 ggcgctggcg gtaaagccac caaagatctt gtctttccat gcctgggtgt accagggttt
     6961 gctggaggca tcggcgaact tcttgaactg ccagctcacg ctgcccatgt aggtgggcga
     7021 gccaaagatg atggcgtccg ctgcggccag ggtgtcccag gcagactccg gcacgttgcc
     7081 gtccgcgtcg atggtgatca gctcaccgcc cgcaccttgc gccaccgatt gcgccatgcg
     7141 ctgggtgtgg ccataaccgg aatgaaaaat cactgctact tttgccatgt tcatgctcct
     7201 tgtaaacaac taaaaaaact cagtcctgca ggtcaaacac cagcacctcg gcgtccttgc
     7261 cattggccag ggtgatccgg gactcttcgt gcaacagtgc cgcgtctcca gactgcagca
     7321 cggtgccatt gacttccagc acaccgcgca ccagatgcac atagcccttg cgcctggctc
     7381 ccagttccag ggtggccgtc tggtcgccgt cgaacaggcc gctgtacatg cgtgcatcgg
     7441 catggatctt caccgagccc tgcgcagcgt ccggcgacgc caccaaccgc agtacgccct
     7501 gcttctccgt gtcagcaaac gtcttctgtt cgtagctggg atcaatgcct ttcacattgg
     7561 gctcaatcca gatctggaga aagtgggtag tgtcgttggg cgcatggttg aactcgctgt
     7621 gctgcacgcc gctgccagcg ctcatgcgct gcacgtcacc cgggggaatg cccttgacat
     7681 tacccatgct gtccttgtgc gccagattgc ccgagatgac gtagctgacg atttccatgt
     7741 cgcggtggcc atgggtgcca aagccggtgc ccggtgcaat gcggtcttcg ttgatcacgc
     7801 gcaggttgcc ccagcccatg tgcttggggt cgtagtaccc ggcaaaggaa aaactgtgga
     7861 acgatttgag ccaaccgtgg tcagcgtatc cccgttcctg ggatgtgcgt agtgtcagca
     7921 tctgtcttct ccgttgtgta ctgcgatgga ctgaacttta gggcgtgaac ctcccaatac
     7981 catgcacccc aattgatggc atcattcaaa tggtttcaat caaactatca ccatcatgca
     8041 aaacgcacgc gatttcctga ccccggatgc gctgtccatg ctgcaggcca tcgccaaggc
     8101 cggcagtttt gccgcagcgg cgcgcgagat gggcatggtg cccagcgcgc tcacctaccg
     8161 ggtgcgccag atggaggacg cactggatgt gctgctgttt gaccgcagtt cgcgccaggc
     8221 ccggctgacc gaagccggct cggagctgct gcgcgagggc acccgcctgc tgaccgagat
     8281 cgatgcggtg gccaaccgcg tcaagcgcgt cgccaccggc tgggagagcc agttcaccat
     8341 cgcagtggac accatcattt cccgatccac cgtcatggag ctctgcgacg cgttctacca
     8401 gctggccccg cccacccgca tccgcctgcg cgacgaaacg ctttcgggca cgctggaggc
     8461 actgacatcc gggcaggctg atctggccat tggcgccgga cccgacagtg ccacggctgc
     8521 cgacttgcac agcaaaccgc tgggcaacgt caccttcgtt tacgccgtgg ccccccacca
     8581 tccgctggcc catgcccccg agccgctgag tgacgccgtg atccagaagc accgtgcggt
     8641 agcggtggcc gactcggtgc agcgcggtgg cggccaaacc ttcggcctgc tggctgggca
     8701 ggacgtcttc acggtggcca gcatgcaggc caaactggat gcgcaactgc gcggcatggg
     8761 gggcggcaac ctgcccctct gcatggccaa tgccttcatc gataccgggc gcctggtcgt
     8821 caagaaaacc gagcgcgggc agcgccaggt cagcgtgcat tacgcgtggc ggggcggcaa
     8881 gtccaacacc tcgggacggg cgctccagtg gtggctgcaa cagcttgaga gcccagccac
     8941 ccgggccgcg ctgctggaaa atcaccgcag catgtaatgc ccgacggtgc cgccagcgtg
     9001 tataaagcca ataaaccaca actttcggag aacgcccgca tgaccacttc cagacacatc
     9061 gccatcgtgg gggccggcat cgcagccatt acctgcgcac gcaccctggt gcaggcaggc
     9121 catcgggtca ctgtgtttga aaagagtcac agtgtgggtg ggcgcatgtc cacccgcagc
     9181 agcccgtttg gaaccttcga ccacggcgcg cagtacttca cggtacgcga tccccgtttc
     9241 gaactggccc tgcaaaccac accgggcctg tgccgcccct ggagcgccaa caccgtgcgc
     9301 gtgctggact cgcacggccg cgttgccgcc gccggcctgc ccgcgcgcga accgcactgg
     9361 gtacccaagc ccggcatgaa cgcattggtg cgccaatggg cccagccgct ggccgatgcc
     9421 gggcgcatcg agttgcaaac caaagtgact gccatcgaac cggactcgct gaacgccaaa
     9481 cagtggcaat tgcgcacgca gggcgaaggc gacagcagcc atgtatttgg cggttttgac
     9541 gccgtggtgc tggccattcc caacgtacag gccagtgcgc tgctacagtc cagccccaag
     9601 ggcgcggcgc tggccaagaa aatctcccgc gtgaccgtgg ctccgtgtct gaccctgatg
     9661 ttggcattcc cgcaagcggt gcagccgggc ctcaccaccc tgggcccgca atggagcgcc
     9721 gcccgcagca cccaccaccg cattgcgtgg ctggcccgcg aatcgagcaa gccgggccgt
     9781 ggcgcggtgg agcgctggac cgtgcaggcc agtgccgcct ggtcgcagga acacctgcac
     9841 gatgacgtcg accggataga agccaaactc atcaaggcct tttccgaagt caccggcatc
     9901 cgcgccgaac ccgcccatgc ggatgtgcac cgctggatgt atgccaagac cgagctggcc
     9961 ttgggcgaga gccacctgtg ggacggcaag gcaggcattg gcgtgtgtgg cgactggtgc
    10021 ctgggccacc gggtcgaaga cgctttcgtt tccggcttgg aattggccct gaagatcgtt
    10081 tagcccccac gctccgccgc tgcgcgggtc gctgcccccc aagggggccc tcacgccttg
    10141 gggcggccct gcggcgctca ccctatgcat tcgacacgca atagctctac ctaccggggc
    10201 cggttcgccc cctcccccac aggcctgctg catgctggtt cgctcgttgc cgcattggcc
    10261 agttggctgg atgcccgcgc ctacggtggg aagtggctgg tacgcatcga agatgtggac
    10321 acgccgcgct gcgttccggg taccgaccag ctcatcctgc agcagctcgc cgcctgcggc
    10381 ctgcaacccg acgagccccc cgtgtggcaa agccagcgca ccgcgctgta ccagagggca
    10441 ctggaccagt tggtggcgcg aggcatggcc tacgcttgtg cctgctcgcg caaggacata
    10501 gaagcggcac tggcgcaatc tggccacagc aaaccgcgca acggcgaact ggtctacccc
    10561 ggcacctgcc gcgaaggact gcacggcaag cctgcgcggg cctggcgctt tcgcaccagt
    10621 aatttggtgt caaatcaggc tccagccccc gtggaatatg cacaagtagc tactgaaaat
    10681 atagcatcca gcaagtcaga ggtatggtgg actgaccgcc gtctgggcct gcagcagcaa
    10741 gatgtggaac gcgacgtggg agatttcgtc ctgctgcgcg ccgatggact gttcgcctac
    10801 cagctggctg tggtggtgga tgacgcccag cagggcatca cccatgtggt gcgcggcgaa
    10861 gacctggcgg acaacaccgc acgacaaatc ctgctgcaac gggccttgca cctgccgaca
    10921 ccccagtatc tgcatacgcc gctggtgctg ggccccaatg gcgagaaact gtccaagcaa
    10981 aacggtgcgt cgcccgtgga cacgtccacc ccggatgcgg cctttgcagc cctttgccag
    11041 gccgctgccg cgctggattt actgcccgcc gctggcacgc cggccgacgc gctagcgctc
    11101 tggacgcgcc agtggggcga caggtacagc ctgtagcgac cgctgcaccg acgtttcttc
    11161 ctgtatggcc tgctc
//
LOCUS       DAOSJM010000026        11129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5789_length_11129_cov_4.336373, whole genome shotgun sequence.
ACCESSION   DAOSJM010000026 DAOSJM010000000
VERSION     DAOSJM010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5789_length_11129_cov_4.336373"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PKX82_01780"
     CDS             <1..534
                     /locus_tag="PKX82_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOF51005.1"
                     /translation="VGQHQMWAAQYYRFDEPRRWINSGGLGTMGVGIPYAMGIKLAKP
                     DSEVFCITGEGSVQMCIQELSTCLQYNTPIKICALNNRYLGMVRQWQELDYEGRYSHS
                     YMDALPNFVKLAEAYGHVGMLIERPQDVEPALREARKLKDRTVFMDFRTDPTENVFPM
                     VQAGKGITEMLLGSEDL"
     gene            633..1124
                     /gene="ilvN"
                     /locus_tag="PKX82_01785"
     CDS             633..1124
                     /gene="ilvN"
                     /locus_tag="PKX82_01785"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOF51006.1"
                     /translation="MKHIIAVLLENEPGALSRVVGLFSARGYNIESLTVAPTEDPSLS
                     RMTIETHGSDDVIEQITKHLNRLIEVVKVVDLTEGSYIERELMMVKVRAVGKEREEMK
                     RMADIFRGRIIDVTEKSYTIELTGDQSKNDAFLEAIERAAILETVRTGSSGIGRGERI
                     LRV"
     gene            1154..2167
                     /gene="ilvC"
                     /locus_tag="PKX82_01790"
     CDS             1154..2167
                     /gene="ilvC"
                     /locus_tag="PKX82_01790"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOF51007.1"
                     /translation="MKVFYDKDCDLSLIKGKTVAIIGYGSQGHAHAQNLNDSGVKVVV
                     GLRKGGASWDKVAKAGLKVAEVNEAVAAADVVMILLPDENIAGVYKEIESSLKQGASL
                     AFAHGFNVHYNQVVPRADLDVWMVAPKAPGHTVRNTYTQGGGVPHLVAVHQDKSGKAR
                     DLALSYAMANGGGKAGIIETNFKEETETDLFGEQAVLCGGAVELIKMGYETLVEAGYA
                     PEMAYFECLHELKLIVDLIYEGGIANMNYSISNNAEFGEYVTGPEVINEQSRAAMRNA
                     LKRIQNGDYAKMFIQEGRLNYPSMTARRRNTADHQIEVVGAKLRAMMPWIAKNKLVDQ
                     TRN"
     gene            2334..3149
                     /gene="pssA"
                     /locus_tag="PKX82_01795"
     CDS             2334..3149
                     /gene="pssA"
                     /locus_tag="PKX82_01795"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOF51008.1"
                     /translation="MHDGIDSNETHNDAVVIRKRRKGIYVLPNLFTLAALFGGFYAIV
                     MAMNGRFDLAAVGVFCAMVLDSLDGRVARMTNTQSAFGEQMDSLSDMVSFGAAPALIS
                     YVWALKGLGRWGWFAAFVYCACAALRLARFNVNTTVVDKRYFQGLPSPAAAALVAGFI
                     WLATDLGMQGPDLAWPMFALALYAGLTMVTNVPFYSFKDISMKRSVPFAVIVLIALGI
                     AVINIDPPTVMFSVFVVYGLSGYVVYGVRKARGVQTSVISTSTDEPDERGLHK"
     gene            3359..4897
                     /locus_tag="PKX82_01800"
     CDS             3359..4897
                     /locus_tag="PKX82_01800"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOF51009.1"
                     /translation="MADKLIIFDTTLRDGEQSPGASMTRDEKLRIARQLERLKVDVIE
                     AGFAASSNGDFEAVQLIANTIKDSTVCSLSRANDRDISRAAEALKGADRARIHTFIAT
                     SPLHMEKKLRMTPDQVFEQAKQSVRFARNLVGDIEFSPEDGYRSDMDFLCRVLEAVIT
                     EGATTINVPDTVGYAVPELYGNFIKTLRERIPNSDKAIWSVHCHNDLGMAVANSLAGV
                     KIGGARQVECTINGLGERAGNCSLEEVVMAVKTRKDYFGLEVGIDTRHILAASRMVSQ
                     TTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMRAEDVGWTANKIVLGKLSG
                     RNAFKQRLQELGVQMDSEAEVNAAFTRFKELADRKSEIFDEDILALVSEENVTNDKEQ
                     YAFVSLSQHSETGERPQATIIFTVDGKEVTGTSEGNGPVDASLKAIEIHAKSGAEMVL
                     YSVNAISGSTESQGEVTVRLQNSGRVVNGVGADPDIVVASAKAYLSALNKLQSKADRV
                     AAQG"
     gene            5091..6077
                     /locus_tag="PKX82_01805"
     CDS             5091..6077
                     /locus_tag="PKX82_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOF51010.1"
                     /translation="MVALPAHAGVERGHSSGVKRGVVKKPVAKANVGRKRAVAQVATP
                     RQLSFGQLAGLHGASDALALRSSVALVVDQDTKEVLFSKNESAVLPIASITKLMTGLL
                     VSEARLSMDEMITISQEDVDTEKGSSSRLRVGTELTRGELLHLALMASENRAAHALGR
                     TYPGGMATFVGLMNARARMLGMNDTRYVEPTGLSSRNQSSARDLATLVNFAYGDPVLR
                     ELSTSTGYQVEVGHRTLQYNNTNRLVKNPAWDIGLQKTGYISEAGQCLVMQAKVAGRK
                     LIMVFLDSAGKLSRIADAERVKRWIESNPASLTRATPLAAREPEHRVAVNRN"
     gene            complement(6074..6865)
                     /locus_tag="PKX82_01810"
     CDS             complement(6074..6865)
                     /locus_tag="PKX82_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOF51011.1"
                     /translation="MAKKDPDIPTTPAVQVIERIFTLIDVLASREEAISLKEISEKAG
                     LHPSTAHRILNDLATGRFVDRPQPGSYRLGMRLLELGNLVKGRLNVRDAALIPMRELH
                     KLIQQPVNLSMRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAADDPQ
                     RIRAYATRTGLQGHTKNSITTVDALERELSRVRQYGQASDNEELELGVRCMAAGIYDD
                     QNKLVAGLSISAPSGRLDDAWLPKLQETAREISSALGHKPGPGRY"
     gene            complement(6883..7629)
                     /locus_tag="PKX82_01815"
     CDS             complement(6883..7629)
                     /locus_tag="PKX82_01815"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HOF51012.1"
                     /translation="MTDLKDLPLQALQFYATAPYECSYLPDRLARSQVATPSYLINNS
                     TYSELVSKGFRRSGMFTYRPYCDGCQSCTPLRVIATEFQPDRSQKRAWAKHQHLVPRV
                     LRLGFEQDHYALYLQYQAERHAGGGMDEDSVSQYTQFLLQSRVNSRLVEFREPTADGS
                     LGALKMVSILDVLDDGLSAVYTFFDAQERGSYGTYCVLWQIEQARKLGLQYVYLGYWI
                     AQSPKMSYKERFRPFQTLRSGQWSAGDGPS"
     gene            complement(7640..8389)
                     /gene="aat"
                     /locus_tag="PKX82_01820"
     CDS             complement(7640..8389)
                     /gene="aat"
                     /locus_tag="PKX82_01820"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOF51013.1"
                     /translation="MSTPKIPLPWLEPSDSFPPVENAWGPDSDAPGLLAAGADLSVPT
                     LSRAYANGIFPWFSEGQPVLWWSTNPRMVLTVANFKLHHSLRKTLKNFVRDARCEIRF
                     DSAFAKVIQSCAHAPRRGHAGTWIVPAMVDAYTQLHAAGLAHSVETWIDGELAGGLYC
                     VALGKAVFGESMFANRTDASKIALAALVAFCRAQGVTLIDCQQNTSHLASMGAAEIPR
                     TVFVDQVQHARFQPPLPWHFDPLYWNHILGS"
     gene            8410..8486
                     /locus_tag="PKX82_01825"
     tRNA            8410..8486
                     /locus_tag="PKX82_01825"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8444..8446,aa:Pro,seq:tgg)
     gene            8539..8615
                     /locus_tag="PKX82_01830"
     tRNA            8539..8615
                     /locus_tag="PKX82_01830"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8573..8575,aa:Arg,seq:tct)
     gene            complement(8679..9512)
                     /gene="murI"
                     /locus_tag="PKX82_01835"
     CDS             complement(8679..9512)
                     /gene="murI"
                     /locus_tag="PKX82_01835"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOF51014.1"
                     /translation="MIDTSCPIGVFDSGVGGLSVLRALRAELPQEQFVYVADSGHAPY
                     GERNDEHVIARTHAIALHLLERHRIKALVVACNTATAAAIRLLRDTHPGLPIIGIEPA
                     LKPAAQTSKTGVIGVMATRGTLNSEKFRALLQSLHGKATFVLQPCDGLANAIECDDAM
                     KIEALCADYTGAMGQFGLNSGQMDTLVLGCTHYPFATPQLRAHVGPDIPFLEGGAPVA
                     RQTRRLLAEIHGLFTEPALAHCPRDPMLMLCTTGDPGALRMALERWLDLRVEVSQVAI
                     P"
     gene            complement(9509..11065)
                     /locus_tag="PKX82_01840"
     CDS             complement(9509..11065)
                     /locus_tag="PKX82_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOF51015.1"
                     /translation="MTTTTIRQEDLIESIAGALQYISYYHPTDYIAHLARAYEREKSP
                     AAKDAIAQILTNSKMSATGQRPICQDTGIVNVFLKVGMDVRWEGFTGSLDDAINEGVR
                     QGYNHPDNTLRASVVADPEFLRKNTKDNTPAVIFTEIVPGNTVEVTVAAKGGGSENKS
                     KMYMLNPGDSVVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKSVLMAKESLMDDLDMY
                     ELQAKAASGAELDNVEKLRLELFEKVNALGIGAQGLGGLTTVLDVKIKMYPTHAASKP
                     IAMIPNCAATRHAHFVMDGSGPVYLTPPSLDTWPDVAWTPDYVKSKKVDLNTLTKEEV
                     ASWKPGQTLLLNGKMLTGRDAAHKRIQGMLAKGEKLPVDFTNRVIYYVGPVDPVAGEA
                     VGPAGPTTATRMDGFTEMMLAETGLISMVGKAERGPVAIEAIKKHKSAYLMAVGGAAY
                     LVSKAIRAAKVVGFADLGMEAIYEFDVVDMPVTVAVDAGGTSAHITGPAEWQKKIASG
                     EFKTIPVKSA"
BASE COUNT         2271 a         3042 c         3390 g         2426 t
ORIGIN      
        1 gtaggtcagc accagatgtg ggcagcgcag tattaccgct ttgatgagcc gcgtcgctgg
       61 atcaattcgg gagggctggg gaccatgggc gtcggtattc cttacgccat gggtatcaaa
      121 ctggccaaac ctgacagcga ggtgttttgc attacgggcg aaggttcagt gcagatgtgt
      181 atccaggagc tctcgacctg cctgcagtac aacactccca tcaagatttg tgccctgaac
      241 aatcgctatc ttggcatggt ccgccagtgg caggagttgg actacgaagg tcgctatagc
      301 cacagctata tggatgcctt gcccaacttc gtcaagctgg cagaggccta cggccacgtt
      361 ggcatgctca tcgagcgtcc gcaagatgtt gagccagcat tgcgtgaggc gcgcaaactc
      421 aaggaccgta cggtgtttat ggacttccgc acggacccca cagagaatgt gttccccatg
      481 gtgcaagccg gcaagggcat tacggagatg cttttgggat ctgaagatct ctgaagaccg
      541 ctttactatc cattttttga cgaatctatt gtctgccaag ccctgcagtt accgccgcag
      601 ggggagggca gcgaaaagag gaatcggtac ctatgaaaca catcattgca gttttgctgg
      661 agaacgagcc cggagctctt tcccgcgttg tgggcctttt ttcggcccgt ggctacaaca
      721 ttgaatccct gacggttgca ccgaccgaag accccagtct gtcgcgcatg accatcgaaa
      781 cccatggttc tgatgatgtc atcgagcaaa tcaccaaaca cctgaatcgc ctgatcgagg
      841 ttgttaaagt ggtggatttg acggagggct cttatatcga gcgtgagctc atgatggtta
      901 aagtccgcgc agtcggaaaa gagcgtgagg aaatgaagcg tatggcggac atcttccgtg
      961 gccgcatcat tgacgttacg gaaaagagct acaccatcga attgactggt gaccagtcca
     1021 agaacgatgc atttctcgaa gccatcgagc gtgcagcgat tctggaaact gtgcgcacag
     1081 gatccagcgg catcggtcgt ggcgagcgca ttctgcgcgt ctgatttttt actttttaca
     1141 acggagagaa gagatgaaag ttttttacga taaagattgc gacctcagcc tgatcaaggg
     1201 taagactgtt gccatcattg gttacggcag ccagggccat gcccacgcac aaaacctgaa
     1261 tgacagcggc gtgaaggtcg tggttggtct gcgtaagggc ggcgcatcct gggacaaagt
     1321 ggccaaggcc ggtctgaaag tcgctgaagt caacgaggca gttgcggccg ccgacgtggt
     1381 catgattttg ttgcccgacg agaatatcgc tggcgtctac aaggaaattg aatcctccct
     1441 caaacaaggt gcctctctgg catttgccca cggcttcaat gtgcattaca accaggtcgt
     1501 gcctcgtgct gatctggatg tgtggatggt cgctcccaag gcgcctggac ataccgtgcg
     1561 caacacttac acccaaggtg gtggcgtgcc tcacctcgtg gcggtgcacc aggacaagtc
     1621 cggcaaggcc cgtgacctgg ccctgagcta tgccatggcc aacggtggcg gcaaggccgg
     1681 catcatcgaa accaacttca aggaagagac cgagaccgac ctgttcggcg aacaggctgt
     1741 cctttgcggc ggtgcggttg aactgatcaa gatgggctat gaaactctgg tcgaagctgg
     1801 ctacgcgccc gaaatggctt acttcgagtg cctgcacgaa ctcaagctca ttgtggatct
     1861 gatctatgaa ggcggtatcg ccaacatgaa ttactcgatc tccaataacg ccgagtttgg
     1921 cgagtacgtg accggtcctg aagtgatcaa cgagcaatcg cgcgccgcca tgcgcaatgc
     1981 cctgaagcgc atccagaatg gcgattacgc caagatgttc atccaggaag gtcgtctgaa
     2041 ctatccaagc atgaccgcac gtcgccgtaa caccgccgat caccagatcg aagtggtggg
     2101 tgccaagttg cgcgccatga tgccctggat tgccaagaac aagctggttg accagacccg
     2161 caactaattc gttggttctt gtccgcaaag gccacttcgg tggcctttgt catttggggg
     2221 gatactgcca cctggcgggc gactggctta cacttcgccg agacaatggg acactgcaca
     2281 ttggtgcgtg tcaagggtat cagggtcatg gactccgaac atgagaaaac agtatgcatg
     2341 atggaattga ttcaaacgag acgcacaacg atgcagttgt gatccgcaag cgccgcaaag
     2401 gtatttatgt cctgccgaac ctgtttacgc tggcagcgct ttttggcggc ttctatgcca
     2461 tcgtgatggc catgaatggc cgcttcgatc tcgcggcggt aggggttttc tgtgcgatgg
     2521 tgctggatag cctggatggt cgcgtggcac gcatgaccaa cacccagagt gctttcggag
     2581 agcaaatgga ttcactgtcc gacatggtct cctttggcgc agcacctgct ctgatttctt
     2641 atgtctgggc gctcaaggga ttggggcgtt ggggctggtt tgccgccttc gtctattgcg
     2701 catgtgcggc gttgcgactg gcacggttca atgtgaatac gacggtcgtc gacaagcgct
     2761 attttcaggg tttgccgtca cctgccgccg ccgccctggt cgccgggttt atctggctgg
     2821 cgaccgattt aggaatgcag ggtcctgact tggcttggcc tatgtttgcg ttggcactgt
     2881 acgcaggcct gacaatggtc accaacgttc cgttttacag cttcaaggac atcagcatga
     2941 agcgcagcgt tccttttgcg gtcattgttc tgatcgcgtt gggaattgct gttatcaaca
     3001 tcgacccgcc gaccgttatg ttcagcgtgt ttgtagtcta tggacttagt ggttatgttg
     3061 tctacggtgt ccgtaaggcc cgtggcgtgc agaccagcgt gatcagcaca tccacggacg
     3121 agccggatga gcgggggctc cacaagtaga catcccggcg gaatagcctg attcattttg
     3181 tgctacattg aagtccatgc aatcttccac cctagaccta acactgctac gccaccacgg
     3241 gcggaagaaa tagcgcacgc tttttttcta caggggcccg ttagcattcg caacgggcct
     3301 tttgtgtttt tgcggtgccg gcggggacca agaccattga aataccagga gaaccaccat
     3361 ggctgacaaa ttgatcatct ttgataccac cttgcgtgac ggagagcagt ccccgggcgc
     3421 ttccatgacc cgcgatgaga agctgcgtat tgccaggcag cttgagcggc tcaaggtcga
     3481 tgtgatcgag gctggtttcg cggccagttc caatggtgat ttcgaagcag tccaactgat
     3541 cgcaaatacc atcaaggact ctaccgtgtg ttcgctctca cgggccaatg accgtgatat
     3601 ctcccgggca gcggaagctc tcaagggtgc ggatcgcgca cgcatccata cgtttattgc
     3661 gacttcccct ttgcacatgg agaagaagct gcgcatgacg ccagaccagg tatttgagca
     3721 ggccaaacag tccgttcggt ttgcgcgcaa tctggtgggc gacatcgagt tcagtcctga
     3781 agatggctat cgcagcgata tggactttct ctgccgtgtg cttgaagctg tcatcaccga
     3841 aggtgcaaca acgatcaatg tgccggacac cgtgggctat gccgtgccag aactctatgg
     3901 caacttcatc aagaccttgc gtgagcgcat ccccaactct gacaaggcta tctggtcggt
     3961 tcattgccac aacgacctgg gtatggcggt tgccaattcg ctggcaggtg tgaagatcgg
     4021 tggcgcacgg caggtagagt gcactatcaa cggtctgggc gagcgtgctg gcaactgcag
     4081 cctcgaagaa gtcgtcatgg cggtgaaaac ccgcaaggac tatttcggtc tggaggtggg
     4141 cattgatacg cgccatatcc tcgcagccag tcgcatggtg agccagacta ccggttttgt
     4201 cgtacagccg aacaaggccg tggttggtgc gaatgcattt gcacacgcct ccggtattca
     4261 ccaggacggt gtgctcaagg cgcgggatac ctatgaaatc atgcgcgcag aagatgtggg
     4321 ctggacggct aacaagattg tgctgggcaa gctcagcggc cgcaatgcgt tcaaacaacg
     4381 tctgcaggag cttggtgtgc agatggacag tgaggcagag gtcaatgcgg ctttcacgcg
     4441 cttcaaggag ttggcggatc gcaagagcga gattttcgac gaagacattc tggcgttggt
     4501 gagcgaagaa aacgtgacga acgacaagga gcaatatgct tttgtgtcgc tgtcgcagca
     4561 cagcgagacc ggcgagcggc cacaggccac gatcattttt acggtcgatg gcaaggaggt
     4621 gactggaact tcagaaggaa acgggccagt ggacgcgtcg ctcaaggcga tcgagattca
     4681 cgccaagagt ggtgccgaaa tggtgctgta ttccgtgaat gcgatcagcg ggtccacgga
     4741 gagccagggt gaagtcactg tacgtttgca gaacagtggc cgggtggtca acggcgtggg
     4801 cgcggatcca gacattgtgg ttgcctctgc caaagcgtat ctgtccgcac tgaataagtt
     4861 gcagagcaag gcagatcggg ttgccgcaca gggctaatac gtaacaacaa cagaaaatat
     4921 tcttgaactt taccgtgcaa gtagttgatc ttacgcaact tttttgttta cactgcagtt
     4981 ttgcacgagg ggttgctggc atcgcgccag cagttccgtt tacaaaatag ttgggattcc
     5041 acttgaaacg tctaactttg cacctgagcg ccatcgccat catttggtcc atggtggcac
     5101 tgccagcgca tgctggcgtt gagcgtggtc attcttcagg tgtcaagcgt ggagttgtga
     5161 agaaaccggt cgccaaagcc aatgtgggtc gtaaacgggc tgtggcacag gtggctaccc
     5221 ctcgccaact ttctttcggg cagttggctg gcttgcatgg cgcgtctgac gcgctggcac
     5281 tgcgttccag tgtggcgctg gtcgtagacc aggatacgaa agaagtcttg ttcagcaaaa
     5341 atgagtctgc ggtcctgccg attgcgtcca ttaccaaact gatgacaggt ttgctcgtca
     5401 gtgaagcgcg tctctccatg gatgagatga tcacgatttc gcaagaggat gtggatacgg
     5461 agaaggggag ttcctcacga ttgcgcgtgg gcaccgagct cacgcgcggt gagctcctgc
     5521 atctggcgtt gatggccagc gaaaatcggg cagcgcatgc cttgggccgt acctacccgg
     5581 gcggcatggc tacctttgtc ggtttgatga atgcacgggc caggatgctg ggaatgaacg
     5641 atacgcgcta cgtggaaccc acgggcctgt ccagcaggaa ccagtccagt gccagagacc
     5701 tggcaacgct ggtgaatttc gcatacggtg atccagtatt gcgcgagttg tccacttcga
     5761 ccgggtatca ggtagaagtc gggcaccgca cactgcagta caacaacacc aatcggctgg
     5821 taaagaaccc agcgtgggac attggtctgc aaaagacggg ctacatttcc gaggctggcc
     5881 aatgcctggt gatgcaggcc aaggtggcag ggcgcaagct gatcatggtg tttctggatt
     5941 cggctggcaa actcagccgt attgctgatg cggagcgcgt caagcgctgg attgagtcca
     6001 atccagccag tctgacgcgt gcaacgccgc tggctgcccg cgaacccgag catcgggttg
     6061 cggttaacag gaactaatag cgtccagggc cgggcttgtg tcccagggcg ctggatattt
     6121 cccgcgccgt ctcctgcagt ttgggaagcc atgcatcgtc cagacgccct gacggagcag
     6181 agatggacaa gccagccacc agtttgttct ggtcatcgta aattcccgcc gccatacacc
     6241 gcactccgag ttccagttct tcgttgtcac tggcctggcc gtactggcga acacgcgaaa
     6301 gctctcgctc cagtgcgtct accgttgtaa tgctgttctt ggtatgtcct tgcagacctg
     6361 tgcgcgtcgc atacgcacga atgcgctgcg gatcatctgc ggcgaggaat agcttgccca
     6421 ccgaagtcag atgcagaggc gcccgtccgc cgatggcacg gaccacctgc attcccgagc
     6481 gctcgctgta ggcgcgctcc acatacacaa tttcatctcc ctggcgcatg ctcagattga
     6541 ccggctgctg gatcagcttg tgcagctcac gcatgggtat gagtgctgca tcgcggacgt
     6601 tgagccgtcc cttgaccaga tttccgagtt ccagcagacg catgcccagg cggtaactgc
     6661 caggctgagg ccggtccaca aagcggccgg tggccaggtc gttgaggatg cggtgtgcgg
     6721 tagagggatg caacccagcc ttctcgctga tctccttgag tgagatagcc tcttcgcgcg
     6781 aggcaaggac atcgatcagg gtgaaaatcc gttcaatgac ttgaacggca ggggtggtgg
     6841 ggatatcggg atctttttta gccatggtgt gattgccgaa tcttaactcg ggccatcacc
     6901 tgcagaccat tggccgctac gtagtgtctg aaaagggcga aaccgctcct tgtaactcat
     6961 cttggggctc tgcgctatcc agtagccgag gtagacatac tggagaccaa gttttctcgc
     7021 ctgttcgatt tgccacagca cgcagtaggt gccatagctg ccacgctcct gagcgtcgaa
     7081 gaatgtgtag accgcggaca gaccgtcatc cagaacgtcc agaatcgaca ccatcttgag
     7141 ggcacccagc gaaccatcgg cggtgggctc ccgaaactca accagacgtg agttgacccg
     7201 gctctgcagc agaaactggg tgtattggct gacgctgtct tcgtccatgc cgccgcccgc
     7261 gtggcgttct gcctggtact gcaggtacag ggcatagtgg tcctgctcga agccgagccg
     7321 cagtacacgg ggaaccagat gctggtgttt ggcccaggcg cgcttctggc tgcggtctgg
     7381 ctggaattcg gttgcgatga cccgcaaggg tgtgcaggac tggcatccat cgcaataggg
     7441 ccggtaggtg aacatcccgc tgcgccggaa ccctttggac accaattccg aatacgtcga
     7501 gttgttgatc aggtaactgg gggttgcgac ttgcgaacgc gccagccggt caggcaaata
     7561 gctgcattca tagggtgccg ttgcatagaa ctgcagggct tgaagcggta ggtctttgag
     7621 gtccgtcacg gcgaatagat caactgccca gaatgtggtt ccagtataga gggtcaaaat
     7681 gccacggcag agggggctga aaacgggcgt gttgcacctg atccacaaac accgtgcgcg
     7741 gaatctcggc ggcgcccatg gacgccaggt ggctggtgtt ttgctggcaa tcgatcagcg
     7801 taacgccctg tgccctgcag aatgccacca gagccgccag cgcgatcttg gaggcgtcgg
     7861 ttctgttggc aaacatcgac tcgccaaaaa cagccttgcc caatgccacg cagtacagac
     7921 cacctgcaag ttcgccgtcg atccaggtct ccacgctgtg ggccaggccg gctgcatgca
     7981 gttgcgtgta cgcgtccacc atggccggca cgatccaggt tccggcgtgt ccgcgccgcg
     8041 gagcatgtgc gcaggactgt atgaccttgg caaaagcaga gtcgaagcgg atctcgcaac
     8101 gtgcgtcccg tacaaagttc ttcagtgtct tgcggagcga gtggtgcagc ttgaagttcg
     8161 ccaccgtcag taccatccgc gggttggtac tccaccagag aaccggctgg ccttcgctga
     8221 accagggaaa gataccgttt gcgtaggcgc gtgacagtgt gggaacggac agatctgctc
     8281 cggcggccag caaacccggt gcatccgagt ccggccccca tgcattttcg acaggaggaa
     8341 atgaatctga gggttccagc cacggtaagg gaatttttgg ggtcgacatg cggttagaat
     8401 actagctgtc ggggcgtagc gcagcctggt agcgcatctg ctttgggagc agagggtcgc
     8461 gagttcgaat cccgccgccc cgaccattat tcgatacaaa ggcccttaag tgtcatgact
     8521 ttagggcctt tgtgctttgt gcccgtagct cagtcggata gagcaacagc cttctaagct
     8581 gtgggtcggg ggttcgatcc cctccgggca cgccacttac atttgtccgc gcattcaagc
     8641 gtaagtctaa gcctcccgaa acacttcaga gatcgtgctc agggaattgc cacttggcta
     8701 acttcaaccc gaaggtccag ccaacgctcc agggccatcc gcaaggcgcc cgggtcgccg
     8761 gtcgtgcaca acatgagcat ggggtcacgg gggcagtggg ccagagcagg ctcggtaaac
     8821 agcccgtgga tttcagccag caggcgccgg gtctggcgcg ccacgggggc gccaccttct
     8881 agaaatggaa tgtcgggccc aacgtgcgca cgcagctgtg gtgtggcaaa cggatagtgg
     8941 gtgcagccca gtaccaaggt gtccatctga ccagaattta agccaaattg gcccatagcc
     9001 cccgtgtaat ctgcgcagag tgcttctatt ttcatagcgt cgtcgcactc gatggcgttc
     9061 gccaggccgt cgcacggctg caggacaaag gtggctttgc cgtgcaggga ttgcaacagg
     9121 gcacggaatt tttcgctgtt gagcgttccc cgcgtggcca tgacaccgat gacacctgtt
     9181 ttgcttgtct gggccgccgg cttgagcgcg ggctcaatgc cgatgatggg taaacctggg
     9241 tgcgtgtcac gcagcaagcg gatggctgct gcagtggcgg tattgcaggc gaccaccagg
     9301 gccttgatgc ggtggcgttc cagaaggtgc aaagcaatgg catgcgtgcg cgcaatgacg
     9361 tgctcgtcat tgcgctcgcc gtagggggcg tgcccgctgt ctgccacata gacaaactgc
     9421 tcttgcggca gttctgcgcg cagggcacgc aaaacgctga gtccgccgac cccgctgtca
     9481 aagaccccga tggggcagga cgtgtcgatc aagcggactt gacagggatg gtcttgaact
     9541 cgccgctggc gatcttcttt tgccactcgg ccgggccggt gatgtgggcg ctggtgccgc
     9601 ccgcatccac cgccacggtg accggcatgt cgaccacgtc gaattcataa atggcttcca
     9661 tgcccaggtc ggcaaagccc accaccttgg ccgccctgat ggctttggac accaggtaag
     9721 cggcgccgcc cacggccatc aggtacgcac tcttgtgctt cttgatggcc tcgatggcga
     9781 ctgggccgcg ctcggccttg cccaccatgg agatcaggcc ggtctcggcc agcatcattt
     9841 cggtaaagcc gtccatgcgc gttgctgtgg tcgggccggc ggggcctacc gcttcgccag
     9901 ctacggggtc caccgggcct acgtagtaga tgacgcggtt ggtgaaatct accggcagtt
     9961 tctcgccctt ggccagcatg ccctggatgc gcttgtgggc tgcatcgcgg ccggtcagca
    10021 ttttgccgtt gagcagcagg gtctgaccgg gcttccagct ggcgacttct tccttcgtga
    10081 gcgtgttcag gtcgactttt ttgcttttca cgtagtcagg ggtccaggcc acgtcgggcc
    10141 aggtgtccag cgacggaggg gtcagataca cgggaccgga gccgtccatc acaaagtggg
    10201 cgtggcgggt ggcggcgcag ttggggatca tggcaatcgg cttgctggcg gcgtgggtcg
    10261 ggtacatctt gatcttgacg tccagtaccg tggtcaggcc gcccagaccc tgggcgccaa
    10321 tgcccagcgc gttgactttt tcgaacagct ccagacgcag tttttccacg ttatcgagtt
    10381 cagctccgct ggcagctttt gcctgcaact cgtacatgtc cagatcgtcc atcaggctct
    10441 ctttggccat caagacagac ttttctgccg taccgccaat gccgatgccc agcatgcccg
    10501 gcgggcacca gccggcgccc atggtcggca cggttttcag cacccagtcc accaccgagt
    10561 cgccggggtt aagcatgtac atcttgctct tgttttcaga gccgccgccc ttggcggcca
    10621 cagtcacttc cacggtgttg ccgggcacga tttctgtgaa gatcaccgcc ggcgtgttgt
    10681 ccttggtgtt cttgcgcagg aactcggggt cggccacgac cgaggcgcgc agcgtgttgt
    10741 cggggtggtt gtagccctgg cgcacgcctt cgttgatagc gtcgtccagg ctgccggtga
    10801 agccttccca gcgcacgtcc atgccgactt tcaggaacac gttgacgatg ccggtgtcct
    10861 ggcagatggg gcgctggccg gtggcgctca tcttgctgtt ggtcaggatc tgggcgatcg
    10921 catccttggc cgcagggctc ttttcgcgct cgtaggcgcg ggccaagtgg gcgatgtagt
    10981 cggtggggtg gtagtagctg atgtactgca gcgcgccggc aatggattcg atcaggtctt
    11041 cttggcgaat ggtcgtcgtg gtcatggcgg tgggggtttg agggaaaacg cgaaccccga
    11101 attatccccc gccgggcttg cgcggtcct
//
LOCUS       DAOSJM010000027        10896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5930_length_10896_cov_4.236602, whole genome shotgun sequence.
ACCESSION   DAOSJM010000027 DAOSJM010000000
VERSION     DAOSJM010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10896
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5930_length_10896_cov_4.236602"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /gene="rlmN"
                     /locus_tag="PKX82_01845"
     CDS             complement(<1..855)
                     /gene="rlmN"
                     /locus_tag="PKX82_01845"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOF51016.1"
                     /translation="MTANLLDYDLEGLASFCERLGEKRFRATQLFRWIHQKGAAQFDD
                     MSDLAKSLREKLKTSARIQAPQVISQHISSDGTIKWLFDVGGGNAVETVFIPEDDRGT
                     LCVSSQAGCAVGCRFCSTGHQGFSRNLTVAEIVGQLWFAEHFLRGHLHSSERVISNVV
                     MMGMGEPLQNYAALVTSLRVMLDDHGYGLSRRRVTVSTSGVVPMIDRLGQDCPVALAV
                     SLHAPNDALRDNLVPLNRKYPIAELLDACQRYLAFAPRDFITFEYCMLDGVNDTPDQV
                     RALAARLGH"
     gene            complement(863..1288)
                     /gene="ndk"
                     /locus_tag="PKX82_01850"
     CDS             complement(863..1288)
                     /gene="ndk"
                     /locus_tag="PKX82_01850"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOF51017.1"
                     /translation="MTIERTLSIIKPDAVAKNVIGQIYARFEAAGLKVVAARMAHLSR
                     GEAEAFYAVHKARPFFKDLVDFMISGPVMIQALEGENAILKNRDLMGATDPKKAAPGT
                     IRADFADSIDANAVHGSDAAETAAVEVAFFFPGMNVYSR"
     gene            complement(1364..2668)
                     /locus_tag="PKX82_01855"
     CDS             complement(1364..2668)
                     /locus_tag="PKX82_01855"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOF51018.1"
                     /translation="MNDNTPQENTPDEVGVPAEMPAVELAVSEGIRDARGLVAGQPGD
                     ANRFGDIISGQFDADEEIPDLAPPKRVLAPMAETPKLHKVLAQAGMGSRLEMEQLILE
                     GRISVNNEPAHIGQRIQFGDSIKVNGKPIRVRIDPPPARVIAYHKPVGEVVTHDDPQN
                     RPTVFRRLPKLQNGKWQSVGRLDLNTEGLLLFTSSGELANNLMHPRFGLEREYAVRVL
                     GALNKEEKQTLLDGVTLDDGVAQFGSIEEGGGEGSNCWYRVTISEGRNREVRRMFEAV
                     GHAVSRLIRIRYGAMLLPRGLKRGAWMELDDADIRSLVQAAGGKRPPAAAGEAGLQDQ
                     RLRENRRGGRTGGPRGNGGQRPAGPSNDRGQAPQGRSRTKPGNRGGAERSVDRQAGGA
                     QPDPMKTAFGYIGADSFTRQRQDAGQRNRSGGGGTRRNGRSR"
     gene            complement(2665..3339)
                     /gene="scpB"
                     /locus_tag="PKX82_01860"
     CDS             complement(2665..3339)
                     /gene="scpB"
                     /locus_tag="PKX82_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOF51019.1"
                     /translation="MNLADAKRILETALICSQQPLPVRDMRTLFNESLGVDTLKTLLE
                     DIRNDWAGRGVELVGVASGWRFQSRPEMREYLDRLHPEKPPRYTRATLETLAIIAYRQ
                     PVTRGDMEDIRGVTISSLLIKQLEDRGWVEVIGHREAPGRPALFATTRQFLDDLGLES
                     LDQLPLLESPSQATEVFGSFAEAISEGPISAGDEPAGDAVELQMDMPLEVSESDATLP
                     LNGSTE"
     gene            complement(3457..4458)
                     /locus_tag="PKX82_01865"
     CDS             complement(3457..4458)
                     /locus_tag="PKX82_01865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOF51020.1"
                     /translation="MTPADADAEEGEVSVSEAELRAGTVSEALHGQRLDRALVDMASE
                     FSRSYLQQLIEAGAVTLNGKSLTKASQRVKAGDNLSIELRPTPQSQAFRPEPIPLQVV
                     YADAHILVIDKPAGLVVHPAPGNWSGTLLNALLSYDSESSTIPRAGIVHRLDKDTSGL
                     MVVARSRRAMDALVQAIAARQVSRQYLALGHKPWSGRSEVTVDAPVGRDPRNRLRMAV
                     VDLQSTAGKTAKTDFVHLGSAGGFCFVRCILHTGRTHQIRVHMASLGHPLVADTVYGG
                     SVVPLMGRQALHACRLAFMHPVTGADLVFESPLPPDFAGLVGSLGLSYNAPKRLSSP"
     gene            4500..5306
                     /locus_tag="PKX82_01870"
     CDS             4500..5306
                     /locus_tag="PKX82_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOF51021.1"
                     /translation="MLRVKLSVVYACVLATTTLLLTACSTEPIDKTAGMSPNTLYAEA
                     KDEMGSGQWEKAVPLLEKLEARAAGTPLAQQAQLDKAYAHYKSSEPAQAIATLDRFMK
                     LHPASPAMDYALYLKGIINFNDDLGMFSSVTRQDLAERDQKAAKESFEAFKELVGRFP
                     DSRYAEDARKRMNYIVNSLAQYEVYVARYYYKKGAYLAAINRAQVTVTDYRDVPAMEE
                     ALFILYKSYDALGMTQLRDDSRRVLEANYPQSEFLTKGSKTAADPWWKVW"
     gene            complement(5257..7272)
                     /locus_tag="PKX82_01875"
     CDS             complement(5257..7272)
                     /locus_tag="PKX82_01875"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOF51022.1"
                     /translation="MSLRDAVERAFAADGPLRNAVDAYVPRPGQTQMALEVASTLDEG
                     GVLVVEAGTGVGKTFAYLIPALLSGERVLLSTATKALQDQLFQRDIPRLMAVLGIAVR
                     VALLKGRGSYLCLHRMAQARDALPARDMRSIQSLAQVELWAQTTRFGDLAELVHLEDG
                     SPVIPLVTSTRDNCLGAKCPQAQACHVNLARREAMAADLVVVNHHLFFADLSVRESGV
                     AELLPTVRSVVFDEAHQLSEIGVQFMGQQFSTGQLASFSTELFKVSQQHARGFAPWDE
                     LVHELQRAGQALHAAGGARAGRRRWRGAAPEGMEPAEWEAVVCMLRDAMEAVQRALAA
                     VAEVSPELSALHARAQHLTSSLELFLAPVEPGYVRWIDTGNQVRLVHSPLDIADTMRD
                     RVLNPESPTGRKSWIFTSATLGHDASLGWFLQSCGLEGACVLQVPSPFDYPSQAALYI
                     PQDMPKPSDVTHTQAVAQIVAEGAHMLGGRTMVLTTTLRAMRSIGEALRDIFGLAGGM
                     DVLVQGEASKWELVERFCQAERVGGAACILVASASFWEGIDIPGEALQLLVIDKLPFA
                     PPDDPLVQARSDQLEASGRSPFKDLHVPQAAVALKQGAGRLIRRETDRGILVVCDVRL
                     RQMGYAKKLLAALPPMRRLDSKDQWLEALTDLTRPSTMDPQRFSNPW"
     gene            complement(7276..7479)
                     /locus_tag="PKX82_01880"
     CDS             complement(7276..7479)
                     /locus_tag="PKX82_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOF51023.1"
                     /translation="MESNLHSPERRLIELRMEHGDLDALIDSAAAHVPVDELMMRRLK
                     KKRLALRDEIARLERELDPNEPA"
     gene            7592..8356
                     /locus_tag="PKX82_01885"
     CDS             7592..8356
                     /locus_tag="PKX82_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOF51024.1"
                     /translation="MSIGYKLTASTGIHKGDREYQQDQVALIKHPRINGCILAIVADG
                     MGGRSGGRKASDQVMMTARQLFERYDPATDDASATLNQIVQEAHIVIKLTAISAEEEP
                     HSTMAAFLINPRGDCHWAHTGDSRIYQYHGAQFIKRTMDHSYVQALVNRGELTEEQAA
                     VHPQSNILMGCLGTDNDPPVDNHFIAQLRPGDVLMACSDGIWHYFNTDEIGSVLSTLS
                     AREATEFLIEKARTRSHGGGDNLSLVIVKLEPLDKR"
     gene            complement(8353..8940)
                     /locus_tag="PKX82_01890"
     CDS             complement(8353..8940)
                     /locus_tag="PKX82_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51025.1"
                     /translation="MTRWWLALVFLLAAGQFHAHAQQGTSAGVSDEQRRLDALRQEFT
                     RQSQEEESACQQRFAVNDCLKTVQARYRAKLADIKKQEAGLHDRQRQQRAEEQSQRAE
                     KRSRAREQQLQEQSATADRSSREDRPQKNAAGIATAPKVPASAPRPAAREPSPQGREA
                     HARKLREAEDRRAARDKRVKEKAAGKQPIPLPTAP"
     gene            complement(8937..10040)
                     /gene="zapE"
                     /locus_tag="PKX82_01895"
     CDS             complement(8937..10040)
                     /gene="zapE"
                     /locus_tag="PKX82_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HOF51026.1"
                     /translation="MRASVKKTYEAELKARGYTADPAQLRAVEALDRCAGEWAAYKDK
                     RSNAFKKLINRPDIPRGVYMYGGVGRGKSFLMDCFFNAVPLKRKTRLHFHEFMREVHR
                     ELADLKGTVNPLDVLAKRMSLRYKLICFDEFHVADITDAMILHRLLAALFENGVGFVT
                     TSNFKPDDLYPGGLHRDRILPAIALLNDRLEVLNVDNGTDYRQRTLEAVQMYHCPLGP
                     QADQAMEQAFNALAESHDEDPVLHIEAREIRSLRKAGGIVWFDFKTLCGGPRSQNDYL
                     EIASRFHTVLLSNVPHMPVRLASEARRFTWLVDVLYDRRVKLIMSAAVPPEELYTEGP
                     MAHEFPRTVSRLNEMQSREFLALEHRLVDTGLT"
     gene            complement(10115..>10896)
                     /locus_tag="PKX82_01900"
     CDS             complement(10115..>10896)
                     /locus_tag="PKX82_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51027.1"
                     /translation="VFLGAVDEGVAKEAHKAFTKQGLKIELGVKVGEIKNNKKGVSVA
                     YTNAKGEAQTLDVDKLIISIGRVPNTIGLNVEAVGLALDERGAIVVDGDCKTNLPGVW
                     AVGDVVRGPMLAHKAEEEGVAVAERIAGQHGHVNFNTIPWVIYTSPEIAWVGRTEQQL
                     KADGVAYKAGQFPFLANGRARALGDTTGFVKFLADAKTDEILGVHIVGPQASELISEA
                     VVAMEFKASAEDIARICHAHPSLSEATKEAALAVDKRTLNF"
BASE COUNT         2192 a         3494 c         3054 g         2156 t
ORIGIN      
        1 gtggccgaga cgcgccgcca gcgcccggac ctggtccggc gtgtcgttca cgccatccag
       61 catgcagtat tcgaaggtga tgaaatccct gggtgcgaat gcgagatagc gttgacaagc
      121 atccagcagt tctgcaatcg ggtatttccg gttcagcgga accaggttgt cgcgaagcgc
      181 gtcattgggt gcgtgcaggg acaccgccaa ggctaccggg cagtcctgcc caagtcgatc
      241 gatcatgggc accaccccgg aggtcgagac ggtaacgcgc ctgcgcgaca aaccgtagcc
      301 gtggtcgtcc aacatgaccc gcagggaagt taccagcgcc gcatagttct gcaacggctc
      361 ccccatgccc atcatgacca cattcgaaat gacgcgctcg gaggagtgta ggtgtccacg
      421 caaaaaatgc tctgcaaacc acaactggcc cacaatttca gcgaccgtca gattgcggct
      481 aaatccctgg tgaccggtgg agcagaaccg gcaacctaca gcacagccag cctgggacga
      541 cacgcacaat gttccgcggt catcttcagg aataaaaacc gtctcaacgg cattgccacc
      601 gccgacatca aacaaccact taatggtgcc atcggaggaa atatgttggg agatgacctg
      661 gggcgcctgg attcgggctg aggtcttgag cttctcgcgc aaggacttgg cgagatcact
      721 catgtcatca aactgggccg cccctttttg atggatccag cgaaacagct gggttgcgcg
      781 aaaacgcttt tcccccagcc gctcgcagaa agaggccaaa ccctcgaggt cgtaatcgag
      841 cagattggcc gtcattgcat gattaacgcg agtaaacgtt catgcccggg aagaagaatg
      901 ccacttcaac cgcagccgtt tcagcagcat cagatccgtg caccgcgttg gcgtcaatgc
      961 tgtcagcgaa atcggcacgg atcgttccgg gagcagcttt cttggggtca gtcgcaccca
     1021 tcaggtcacg gttcttcaga atggcgtttt caccttccag cgcctggatc atgacgggac
     1081 cggaaatcat gaagtccacc aggtccttga agaaaggacg tgctttgtgc acagcgtaga
     1141 acgcttcagc ttcgccacgg gacagatgcg ccatgcgtgc agccacaact ttgagaccag
     1201 cagcttcaaa acgggcgtag atctgaccga tcacgttttt ggcgacggca tctggtttga
     1261 tgatggaaag agtgcgttcg attgtcatgc tggatagttc ctgaacttaa gaagaaatgt
     1321 ttttgcccct cattggacaa agccgctaat tttagcattc tgtctatcgg ctgcgcccat
     1381 tgcgtcgcgt tcccccacca ccgctgcggt tcctttgacc ggcatcctgg cgctggcgcg
     1441 taaagctgtc cgcaccgata tagccaaaag cggtcttcat ggggtctggc tgggcgcctc
     1501 ctgcctgccg gtcgacactc cgttcagcac caccgcggtt gcccggcttg gtgcgggagc
     1561 gcccctgtgg cgcctgacct ctatcgttcg atgggccggc ggggcgttgg ccgccattgc
     1621 cgcgaggtcc accggtgcgg ccaccgcgcc tgttctcacg cagccgctgg tcttgcagac
     1681 cggcctcccc agcagctgca ggcggccttt tacctcctgc agcctgcacc agggagcgga
     1741 tatcggcatc gtcgagctcc atccaggctc cgcgcttcag accccgaggc aacaacatgg
     1801 caccgtaccg tatgcggatc agacggctaa ccgcatgtcc caccgcttca aacatgcgtc
     1861 gaacctcacg gttccgccct tcggagatcg tcacgcgata ccagcagttg gatccttcgc
     1921 caccaccctc ttcgatggag ccaaactggg caacgccatc gtccagagtc acgccgtcca
     1981 ataatgtctg cttctcctct ttgttcaacg ccccgagaac ccgcaccgcg tattcgcgct
     2041 ccaggccaaa acggggatgc atcaggttgt tcgcaagctc tccagaactg gtgaacaaca
     2101 gtaaaccctc ggtattcaaa tccagccgcc cgacggactg ccatttgccg ttttgaagct
     2161 ttggcaggcg ccgaaatacg gttggccggt tttgcggatc atcatgggta accacctcgc
     2221 caactggctt gtggtacgca atcacgcggg ccggcggtgg atcgatgcgc acacgtatgg
     2281 gcttgccatt gaccttgata ctgtctccga actggatccg ctgaccaatg tgcgccggtt
     2341 cgttgttgac ggaaatacgc ccttccagaa tcaactgctc catttcgagc cgtgagccca
     2401 ttcccgcctg tgcaagcacc ttgtgcagct tcggtgtctc ggccatgggg gccagcaccc
     2461 gcttgggcgg agccagatca gggatctctt cgtccgcatc aaactggccc gaaatgatgt
     2521 ctccgaaacg attggcatcg cccggttgcc cggccacaag gcctctggcg tcccgaatgc
     2581 cctctgaaac agccagctcc actgccggca tctccgcagg cacaccaacc tcatcaggcg
     2641 tgttttcttg tggcgtgttg tcgttcattc tgtagatcca tttaggggta gcgtagcgtc
     2701 tgactcagag acttcaaggg gcatgtccat ctgcaactcc actgcatcac ctgcaggctc
     2761 gtctcctgca gatattggcc cttcgctgat ggcttcagca aacgacccga atacttctgt
     2821 tgcctgggac ggtgactcca gcaacggcaa ctggtccagg gattcgagcc caaggtcatc
     2881 cagaaactgg cgcgtcgtgg caaacaatgc cggacgcccg ggcgcctccc ggtgaccgat
     2941 gacttccacc cagccccggt cctccagttg cttgattaac agcgaactga tggtcacgcc
     3001 ccggatgtct tccatatccc cccgggtcac gggctggcga taggcaatga tggccagtgt
     3061 ttccagcgtc gcccgcgtgt accgcggggg tttttccgga tgaagccggt ccaggtattc
     3121 ccgcatttcc gggcgactct ggaaccgcca tcctgacgcc acaccgacca gctcgacgcc
     3181 ccgccctgcc cagtcgttcc tgatgtcctc cagcagtgtc ttgagcgtgt caacacccag
     3241 cgactcattg aataaggtgc gcatatcgcg caccggtaat ggttgctgcg agcaaatcaa
     3301 tgcggtctca aggatccgct ttgcatccgc caaattcata gtgagacttt ttccgcaaac
     3361 accacccagg aaggtgagaa atcaaatgat tgcatttgga gggcatctgg catgagaagc
     3421 atgctgtgcc tacccatggc aacgcgccgg ggtaattcag gggcttgaaa gccgctttgg
     3481 agcattgtaa ctgagcccca gactgcccac gagtcccgcg aaatccggtg ggagaggtga
     3541 ttcaaagacc aggtctgccc cggtcacggg gtgcataaac gccaggcgac aggcgtgcag
     3601 tgcctgcctg cccatcaatg gaaccaccga tcctccgtac accgtgtcgg ccaccagcgg
     3661 atgccccaga cttgccatgt ggacccggat ctggtgggtg cgcccggtgt gcagaatgca
     3721 gcggacaaag caaaagccgc ctgcgcttcc gaggtgaacg aaatccgtct tcgctgtctt
     3781 gccagcggtg ctttgcaggt caaccacggc catgcgtaac cggttgcgcg ggtcacgccc
     3841 gacaggtgca tccacggtga cctcagaccg cccggaccac ggcttgtgtc ccagtgccag
     3901 gtactggcgg ctgacctgcc tggcggcaat ggcctgcacc agcgcatcca tggcccggcg
     3961 gctgcgcgcc accaccatca gcccgctggt gtccttgtcc agccggtgca cgattccagc
     4021 cctgggtatg gtgctgcttt cactgtcata cgacagcaac gcatttaaaa gcgttccgct
     4081 ccagttgcca ggcgcgggat gcaccaccag ccccgcgggc ttatcaatca ccagaatgtg
     4141 ggcatctgca tacacaacct gcaagggaat gggctctggc cgaaacgcct ggctttgcgg
     4201 cgtgggccgc aactcaatac tcaggttgtc gcccgccttg acacgctgcg atgccttggt
     4261 cagcgatttg ccgttcagcg tgacggctcc cgcctcaatt aactgctgca gataactgcg
     4321 tgaaaactcg gacgccatgt cgacgagcgc gcggtccagc cgctggccat gcaaagcttc
     4381 ggaaaccgtc ccggcacgca actcggcttc agatacggat acttcgcctt cttctgcatc
     4441 tgcgtctgca ggggtgatcg atataatcgc ggcggtctgg ttcaagaggg tttcccacaa
     4501 tgttgcgtgt caaattatcg gtcgtttatg cctgcgtact ggcgaccact accctcctat
     4561 tgacagcttg ctcgacagaa ccgatcgaca agactgccgg gatgagcccc aatacgctgt
     4621 acgcagaagc caaagatgaa atgggctccg ggcaatggga aaaagccgtt ccgctgctgg
     4681 aaaagctcga agcccgggct gcaggcacgc cgctggcgca acaggcacag ctcgacaaag
     4741 cctacgccca ctacaagtcg tccgagccgg cacaggctat tgcaacgctg gaccgattca
     4801 tgaagctcca ccccgccagc ccggcaatgg actacgcgct gtacctcaag ggcatcatca
     4861 atttcaacga tgaccttggc atgttctcca gcgtgacccg gcaggacctt gcagagaggg
     4921 accagaaagc agccaaggag tccttcgaag ccttcaagga gctggtcggc cggtttcccg
     4981 actcgcgcta tgccgaagac gcgcgcaagc ggatgaacta catcgtgaat tcgctggccc
     5041 agtatgaagt ctacgtcgcc cggtactatt acaaaaaggg tgcctacctc gctgcgatca
     5101 accgcgcgca ggtcaccgtg acggactacc gcgatgtccc tgccatggaa gaggcgctgt
     5161 ttattctgta caagtcatat gacgccctgg gcatgacgca attgcgtgat gactccagac
     5221 gtgtacttga agccaactac ccgcagtccg aattcctcac caagggttcg aaaaccgctg
     5281 cggatccatg gtggaaggtc tggtgaggtc cgtcagggct tccaaccact gatctttgct
     5341 atccaacctg cgcatgggtg gcaaagctgc cagcagtttc ttggcataac ccatctgcct
     5401 gagccggacg tcacacacca cgagaatgcc ccggtcggtc tcacggcgga tcagccgacc
     5461 agccccctgc ttgagcgcta ccgctgcctg cggcacatgc aggtccttga aggggctgcg
     5521 tccgctggcc tccagttgat ctgatcgtgc ctgtaccagt gggtcatcag gcggcgcaaa
     5581 gggcagcttg tcaatcacca acagctgcaa cgcctcgcca ggaatatcaa ttccctccca
     5641 gaaagatgcc gacgccacca ggatgcatgc tgccccaccc acgcgctcgg cttggcagaa
     5701 ccgctccacc aactcccact tggatgcctc accctgcacc agcacatcca ttccccccgc
     5761 aagaccaaaa atgtcgcgca atgcctcgcc gatgctccgc atggcgcgca gggtcgtggt
     5821 gagcaccatg gttcgccccc ctaacatgtg agcgccctcg gccacaatct gcgccacggc
     5881 ctgggtgtgt gtcacatcac tcggtttggg catgtcctgc gggatataaa gggccgcctg
     5941 cgaaggatag tcaaacggac tgggcacctg caggacacat gctccttcca acccgcagct
     6001 ctgcaggaac cagcccagcg aagcatcatg ccccagtgtt gccgaggtga atatccatga
     6061 cttgcgcccc gtcggggatt ccggattcag caccctgtcc cgcatggtgt cggcaatatc
     6121 cagcggtgaa tgcaccagac gtacctggtt gcctgtgtca atccatcgga cgtaccctgg
     6181 ctccacggga gccaaaaaaa gttccagact gctggtcaaa tgctgtgccc gcgcgtgcag
     6241 cgcactcaat tccggcgaca cctccgcaac ggctgcaaga gcccgctgca ctgcctccat
     6301 ggcatcgcgc agcatgcaaa ccacggcctc ccactctgcc ggttccatgc cctcgggcgc
     6361 tgcaccacgc catctgcgcc gacccgcgcg cgcaccgcct gcagcatgca gagcctggcc
     6421 agcgcgctgc agctcgtgga ccaattcgtc ccagggcgca aaaccgcgcg catgctgttg
     6481 cgacaccttg aacaattcgg tggaaaaact tgccaattgg ccggtgctga actgttgccc
     6541 catgaactgg acgccaattt cgctcaactg gtgcgcctcg tcgaacacca cggaccgcac
     6601 cgtgggcagc aattccgcca cacccgattc gcgcacgctc aagtcagcaa agaataggtg
     6661 gtgattgacc accaccaggt cggccgccat ggcctccctg cgcgcaagat tgacatggca
     6721 cgcctgggcc tgcgggcact ttgcacccag gcagttgtcc cgtgtagaag tcaccagcgg
     6781 aatcactggc gaaccgtcct ccagatggac cagttcggcg aggtctccga atcgggtggt
     6841 ttgcgcccac agctcgacct gggccagact ctggatcgac cgcatatcgc gtgcgggcaa
     6901 agcatccctg gcttgcgcca tgcgatgcag acacagatag ctgcctcgcc ccttgagcag
     6961 tgccacgcgc accgcgatcc ccagaacggc catcagccgc ggaatatcgc gctgaaacag
     7021 ttggtcctgc aaagccttgg tggctgtgga caaaagcaca cgctcaccac tgagcaatgc
     7081 tggaataagg taagcaaatg tttttccaac accggtaccg gcctccacca ccaggacgcc
     7141 accttcgtcc agcgtgctgg caacttccag tgccatttgg gtctgcccgg gccgcgggac
     7201 gtacgcatcc accgcattgc gcagcgggcc atctgctgca aaggcgcgct ccaccgcatc
     7261 gcgcagactc atgcctcagg ctggctcatt ggggtccagc tcccgctcga gacgggcgat
     7321 ttcgtcccgc aatgccaggc gtttcttttt gagacgccgc atcatgagct catccacagg
     7381 cacatgggca gcggcactat cgatcagtgc atccagatcg ccgtgctcca tgcgcagttc
     7441 aatcagccgc cgctcggggg aatgtaggtt ggattccaat gggagatggg gaaagtacgt
     7501 ggttaaatgc gcgttccatc gaaaagcgct aggtcgataa tacgtccaac gacgttcccc
     7561 gaataaagta ttgctcagca attaatccac catgtccatc ggctacaaac tcaccgcttc
     7621 cacgggcatc cacaaaggtg accgcgagta ccagcaggac caggtcgccc tgatcaagca
     7681 cccgcggatc aatggctgca ttcttgccat cgttgccgat ggtatgggcg gccgaagtgg
     7741 tgggcgcaag gcatcggacc aggtcatgat gaccgcgcgc cagctgtttg agcgctacga
     7801 cccggcgacc gatgatgctt ccgcgaccct gaaccagatc gtgcaggaag cccacatcgt
     7861 gatcaagctg acggccattt ctgcagaaga agaaccgcac agcacgatgg cggctttcct
     7921 catcaacccc cgcggtgact gccattgggc gcacaccggg gattcccgca tttaccagta
     7981 ccacggtgcc cagttcatca agcgcaccat ggaccactcc tacgtccagg ccctggtcaa
     8041 ccgtggagaa ctcaccgaag aacaggccgc cgtacacccc caatcgaata ttttgatggg
     8101 ctgcctggga actgacaatg acccgccggt ggacaaccat ttcattgcgc agttacgccc
     8161 cggcgatgtc ctgatggcct gcagcgacgg catctggcac tacttcaata ccgatgaaat
     8221 cgggtccgtg ctgtcaacgc tgtccgcccg ggaagccact gaattcctga tcgaaaaagc
     8281 cagaacacgc tcccacggtg gcggcgacaa cctgtcgctg gtgatcgtca agctcgaacc
     8341 gttggacaaa cgctagggtg cagttggcag agggatgggc tgcttccctg cagccttttc
     8401 cttgacccgc ttgtctcgcg ccgcccggcg gtcttcggct tcgcgcagtt ttctggcatg
     8461 cgcttcccgc ccctgtgggg agggttccct tgcggcgggc ctgggagcgc tcgcaggaac
     8521 tttgggggct gtagcgatac ctgcagcatt tttctggggc cgatcctctc tggatgaccg
     8581 gtctgcagta gctgactgct cctgcagttg ctgctctcgt gctcggctcc gcttctctgc
     8641 ccgctggctc tgctcttctg cccgctgctg gcgctgccgg tcatgcaagc cggcttcctg
     8701 tttcttgatg tctgcgagtt ttgcacggta gcgggcctga actgtcttga gacagtcatt
     8761 gaccgcaaac cgctgctggc aagcgctttc ttcttcctgc gactgtcggg tgaactcctg
     8821 gcgcaaggca tccagccggc gctgctcatc ggatacccct gccgatgtgc cctgctgcgc
     8881 atgtgcatgg aactgcccgg ccgccagcag gaacacaagt gcgagccacc accgggtcat
     8941 gtcagccccg tatccacaag gcggtgctcc agcgcgagga attccctgga ctgcatctcg
     9001 ttgaggcgcg acaccgtacg gggaaactca tgggccatgg gtccctcggt gtacaactcc
     9061 tccggcggga ccgcagcgga catgatcagc ttgacccggc ggtcgtacag cacatccacc
     9121 agccaggtga agcgccgggc ttccgaggcc aggcgcacag gcatgtgcgg aacattgctc
     9181 agaagcaccg tatggaaccg cgaggcgatt tccaggtagt cattctgcga gcgcgggccg
     9241 ccacacaggg tcttgaagtc aaaccagaca atgccaccgg ccttgcgcag ggaacgaatt
     9301 tcgcgggctt cgatgtgcag caccggatcc tcgtcatgcg attcggccag tgcattgaac
     9361 gcctgctcca tggcctggtc tgcctgcgga cccaatgggc agtggtacat ctgcacggct
     9421 tccagcgtac gctggcggta gtccgtgccg ttgtccacgt tcagcacttc cagccggtcg
     9481 ttcagcaggg caatcgccgg caggatccgg tcgcggtgca gaccgcctgg gtacaaatcg
     9541 tccggcttga agttggacgt ggtgacaaag cccacgccat tctcaaacag cgccgccagc
     9601 aggcggtgca ggatcatggc gtcggtgata tcggcgacat ggaactcatc gaaacagatc
     9661 agtttgtagc gcagggacat gcgcttggcc agcacatcca gggggttgac cgtccccttc
     9721 aggtcggcca gttcgcggtg cacttcacgc atgaattcgt gaaagtgcag ccgcgtcttg
     9781 cgcttcaaag gtaccgcatt gaaaaagcag tccatcagga agctcttgcc ccggccaacc
     9841 cccccgtaca tgtaaacacc acggggaata tcgggccggt tgatgagctt cttgaacgcg
     9901 ttggagcgct tgtccttgta ggcagcccac tcccctgcgc agcggtccag ggcttcgacg
     9961 gccctcagct gcgcaggatc cgcggtgtag cccctggcct tgagctccgc ctcgtaggtt
    10021 ttcttgacac ttgcccgcat actactaaac cgctatcaat acaatagctg cttgcgcaat
    10081 atccacgcgg gctagaagca gattcgacac caaatcagaa gttcagcgtg cgcttgtcca
    10141 ctgccagagc agcttccttg gtcgcttcgc tcaagctggg gtgcgcgtga cagatgcgtg
    10201 caatgtcttc ggcactggcc ttgaactcca tggccaccac ggcttcagag atcaactcgg
    10261 atgcctgcgg acccacgatg tgcacgccca ggatttcgtc cgtcttggca tctgccagga
    10321 acttcacaaa cccggtggtg tcgcccagtg cacgtgcgcg gccgttggcc aggaacggga
    10381 actgacctgc cttgtacgcc acgccgtcgg ccttgagctg ctgctcggtg cggcccaccc
    10441 aggcaatctc ggggctggtg tagatcaccc agggaatggt gttgaagttg acgtgaccgt
    10501 gctggccggc gatgcgctcg gccacggcaa cgccctcttc ttccgccttg tgcgccagca
    10561 tggggccacg caccacatcg cccaccgccc acacgccagg caggttggtc ttgcagtcac
    10621 cgtccaccac aatcgcacca cgctcgtcga gcgccaagcc caccgcttcg acattcaggc
    10681 cgatggtatt gggcacgcgg ccgatggaga tgatgagctt gtccacgtcc agcgtctggg
    10741 cttcgccctt ggcgttggtg taagccaccg acacgccctt cttgttgttc ttgatttcgc
    10801 caaccttcac gcccagttca atcttcaggc cctgcttggt gaatgccttg tgcgcttcct
    10861 tggcgacgcc ttcgtccacg gcacccagaa acactg
//
LOCUS       DAOSJM010000028        10849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_5962_length_10849_cov_4.686122, whole genome shotgun sequence.
ACCESSION   DAOSJM010000028 DAOSJM010000000
VERSION     DAOSJM010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10849
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5962_length_10849_cov_4.686122"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="PKX82_01905"
     CDS             <1..1521
                     /locus_tag="PKX82_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF51028.1"
                     /translation="GVGFDFEQRIVVADAVHWVRLKADVQRGEDGAPHYAVGIAQDIT
                     ERKASESQIQNLAYFDVLTGLPNRRMLMNRLAHAMAVWARHQRKGALLFVDLDNFKVL
                     NDTHGHFLGDMLLQQVAQRLSTCLRQGDTVARLGGDEFVVMLEELSEDTLAATQQARI
                     VAEKILEVLAQPHQLGTYSHLNAASVGVTLFGDFLEGIEEPLKRADMAMYQAKAAGRN
                     TYRFFDPSMHQAIAERANLEKDLREALEGNQFRLVYQPQVNDQAQPTGAEALVRWQHP
                     QRGQVSPADFIPLAEETGLILPLGQWVLETACHQLAAWAKLPAMAHLSVAVNVSPRQF
                     HQPDFADQVLATLERTGASPHRLKLELTEGMLVTNVEDVIARMVRLKSIGVGFSLDDF
                     GMGYSSLSYLKRLPLDQLKIDQSFVRDILMDPNDAAISKMVIVLAESLGLAVIAEGVE
                     TAEQRDYLAGQGCYTYQGYLFSRPLPAVELESFIQSGVTLQVCVPTTIFPVPALRG"
     gene            1476..2258
                     /gene="fliA"
                     /locus_tag="PKX82_01910"
     CDS             1476..2258
                     /gene="fliA"
                     /locus_tag="PKX82_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HOF51029.1"
                     /translation="MRTNDDLSSSGFARLVADKTPMVEKIARNLSRKLPTSVERDDLV
                     QDGMVGLIDAILRWTRETTSSHFDNYVAQRAQGAMLDGLRANDPGSRSLRRAMRAAEQ
                     VIQRLGHQKGRPPLESEVAQALGLDISEYQRMLQDVQGYSLISLEDLGGSDNAQPYLD
                     YCAAFNIDPLRVLERSALRQALLDAIQDLPDQKKQVLRLYYEGELKMHEIGSALGVSE
                     ARVSQLHSHTIAQLRAVILGGEPSPPLLKPRTRKRVDASVAG"
     gene            2255..3580
                     /locus_tag="PKX82_01915"
     CDS             2255..3580
                     /locus_tag="PKX82_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HOF51030.1"
                     /translation="MTSMSGLSITPAVSAGRASEIAQSFDLRDLPADFYANPYPVYAA
                     LRESAPVRQMPDGSCFLTHYADLVAVYRDAQTFCSDKKLEFAPKYNVPPYDAAHPAPL
                     FVHHTTSLVFNDPPLHTRVRKLIMGALTRRAIAEMEPGLVTLVDALLDRMETQGGGDL
                     IEDFASAIPVEIIGNLLGVPHADRAPLRGWSLAVLGALEPRLSPEQQAAGNQAVLDML
                     AYLQGLVAERRRHPGDPEHDVLTRLIQGESSGEQLSEVELLQNCIFILNAGHETTTNL
                     IGNALVALQEHPQAKAGLLSNMQRLAQDPPGLEACLTLAVDEFLRFESSNQLGNRRAL
                     RTCEVGGVALPEGALVTLCIGAANRDPLQFEHPDQLVLARQNNRHLAFGFGIHQCAGL
                     SLARLEGRIAIGRFLQRFPRYALTAPPVRGGRARFRGFLSAPFATDDSV"
     gene            complement(3577..5793)
                     /locus_tag="PKX82_01920"
     CDS             complement(3577..5793)
                     /locus_tag="PKX82_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HOF51031.1"
                     /translation="MSNASRSPSDIFNNEDSNTTANPHFDAVLAARLSRRNVLLGSAS
                     AAATTVLGSAALTGCATAGGAGNGANTLSAPLRSMGFAAVGKSLADKVVVPAGYNVQV
                     IYALGDPLRATTPAFKNDGTDTDWESRAGDHHDGMEYFGLDAQGKPALNATTRALLAI
                     NHEATTDEKLSSFFIHANGGASTLPRPAAEVDKELAIHGLSVVEVSAQNGQWSFNAGS
                     AFNRRVTTMTDVLIHGPARGSEHLVTKFSADATRARGTLNNCGTGKTPWGTFVSGEEN
                     WYGYFFRDAKDDERRGKKDKQVAALNRYGRKAGAASRHGWESAGSDDRFVRWNNGALG
                     ASASADYRNEMNTFGYVVEMDAYDSRQAVRKRTGLGRFAHESMSFAKPVAGQPVVAYM
                     GDDARNEYMYKFVSAAKWDAADANPANRLATGDKYLDKGTLYVASFKDNGTGEWIELS
                     MNNPIIAANPDFEFKSDADVAIFTRLAADAVSATKMDRPEWGGINPRNGEFYMTLTNN
                     SNRTVDGDAGLDAANPRYYSDMKGSKESKGNVHGHIIRVAQANPADTSFKWDIYVFGA
                     ESDADKSRINLSNLTDTNDLSSPDGLVFSEATGLCWIQTDDGAYTDKSNCMMLAAIPG
                     QVGDGGKKTLAYKRTDGSEKRVDTFVGKAPTEDTLKRFLVGPKDCEITGLCETPDGKA
                     MFINIQHPGETTKMADIGDASKYTSQWPANAGYGAGKRPRSATIVITKADGGVIGS"
     gene            complement(5900..6679)
                     /locus_tag="PKX82_01925"
     CDS             complement(5900..6679)
                     /locus_tag="PKX82_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HOF51032.1"
                     /translation="MKLQIVPAGQGVQWVKQGMRTFWRQPLALSGLFFMFMAAMSLLS
                     MVPLLGNILALVILPGATLGLMAATKEALTGKFPMPVLLVSAFRAGKQQLQAMLLLGA
                     IYAAGFLLVLGISALADGGKFAQLYLVGGAMSVELLQAPDFQLAVLLSMALYLPLSLL
                     FWHAPALVHWHGVQPVKSLFFSLVACLRNFWAFTLFGLAWMGVFVGMGTAVSLVASLL
                     GSVEVVSVMMFPLAMLMAAMFFTSLYFTVHDCFEETASDSV"
     gene            complement(6676..7650)
                     /locus_tag="PKX82_01930"
     CDS             complement(6676..7650)
                     /locus_tag="PKX82_01930"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOF51033.1"
                     /translation="MAVYTEVSFDEASTFLASLKLGTLQSMEGCSGGIENTNYFVTTD
                     QAAYVLTLFERLTAEQLPFYLRLMKHLAVHGIPVPDPAANRDGDVLHRLGGKPAAVVN
                     RLRGKSQLAPSAAHCAAVGDMLARMHLAGRSFEMQQPNLRGLPWWNETVPVVLPFLAP
                     EQSALLQSELAYQNLVATLSAYGALPRGPIHADLFRDNVMFAVPTAPDQAPELTGFFD
                     FYFAGVDTWLFDIAVCLNDWCVDLASGTADAERTDAFLDAYGQVRPLQAQERTLLPAM
                     ARAGALRFWLSRLWDFHLPREASMLQAHDPTHFERVLRQRIHQPLVLP"
     gene            7709..10516
                     /gene="polA"
                     /locus_tag="PKX82_01935"
     CDS             7709..10516
                     /gene="polA"
                     /locus_tag="PKX82_01935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOF51034.1"
                     /translation="MSETPNTPRQTLLLVDGSSYLYRAFFAGGDSMSITLPDGTVQKT
                     GAIRIMINMMNSLRKEYPAAYVACVFDAKGPTFRDEIYPQYKAHRDPMPDDLRSQIAP
                     IHEVVRHLGWKVLDVPGVEADDVIGTLAVAAASQGIEVIISSGDKDLAQLVNEHITII
                     DTMNGKRRDLAGVEAEFGVPARLMLDYQTLVGDTVDNVPGVPKVGPKTAVKWLQEYGS
                     LQGVVDNAANIKGVVGENLRNALDWLPTGRTLLTIKTDCDLNGWVPGLPAMDSIAVSA
                     QDTGALATFYEKYGFKGLAKSVAAASTPVRPEPVDGQTRQGERGLFDEPEAATPAPRV
                     SDLVHDTILTWELFDRWFAKLQAAELVALDTETTSLDEMRAEIVGISFSMEPGSAAYI
                     PVAHRYPGAPEQLNREEVLARLKPWLENPAAKKLGQHIKYDRHVFANHGIEVQGYAHD
                     TMLQSYVLEVHMPHGLSSLAERHLGRSGITFEDLCGKGASQICFDQVDIAKAAEYAAE
                     DSDQTLDVHRVLWPQLQANDKLKFIYELEIASSETLYRIERNGVLIDAPTLAKQSHEL
                     GQRILQLETEAYEIAGQPFNLSSPKQLGEIFFDKLGMPVVKKTATGARSTDEEVLEKL
                     AEDYPLPAKLLEHRGLAKLKGTYTDKLAQLALPRTGRVHTHYAQAVAVTGRLSSNDPN
                     LQNIPIRTAEGRRVREAFVAPAGSVIASADYSQIELRIMAHISDDPALLRAFHAGLDV
                     HRATAAEVFGVAVDQVSSEQRRYAKVINFGLIYGMSAFGLARNLGIDNTAAKNYIARY
                     FERYPGVKTYMETTRERAKQQGYVETVFGRRLQLAGIQTAKGAQLAGLERAAINAPMQ
                     GTAADLIKLSMNRVQQVLDAEQRGTKMIMQVHDELVFEVPETEVEWVRTEIPRLMAGV
                     AELKVPLLAEVGVGPNWDKAH"
     gene            10633..>10849
                     /locus_tag="PKX82_01940"
     CDS             10633..>10849
                     /locus_tag="PKX82_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymethylenebutenolidase"
                     /protein_id="HOF51035.1"
                     /translation="MHTNMKTEFDALLPAGTSTEQGATRRTALKAALGVGYAATAMPI
                     MAQTAIKTPSAGLATGEGIYDVDGFMVP"
BASE COUNT         2075 a         3338 c         3496 g         1940 t
ORIGIN      
        1 ggtgtcggtt tcgattttga gcagcgcatt gtggtggcag atgccgtgca ctgggtccgg
       61 ctcaaggccg atgtgcagcg gggggaagac ggtgcgcccc actatgcggt aggcattgcc
      121 caggacatta ccgagcgcaa agcgtcggag tcgcagatcc agaatctggc gtatttcgat
      181 gtgctgacgg gcctgcccaa ccggcggatg ctgatgaacc gcctggccca tgccatggcc
      241 gtttgggcgc ggcaccagcg caagggcgca ctgctgtttg tcgacctcga taacttcaag
      301 gtgctcaacg acacccatgg ccatttcctg ggcgacatgc tgttgcaaca ggtggcgcaa
      361 aggctgtcga cgtgtctgcg ccagggcgac acggtcgcgc ggctgggggg cgatgagttt
      421 gtggtgatgc tcgaagagct cagcgaggac acgctggccg ctacccagca ggcgcgcatt
      481 gtggccgaga aaatcctcga agtgctggcc cagccacacc agctgggtac ctacagccac
      541 ctcaatgcag ccagcgtcgg cgtcaccctg tttggcgact ttctcgaagg tattgaggaa
      601 cccctgaaac gcgccgatat ggcgatgtac caggccaagg cggcaggtcg caacacctac
      661 cgcttctttg accccagcat gcaccaggca attgccgagc gcgccaacct cgagaaggac
      721 ctgcgtgaag cactggaggg caaccagttc cggctggtgt accagccgca ggtgaatgac
      781 caagcgcagc ccacaggagc cgaggcgctg gtgcgctggc aacacccgca gcgcggccag
      841 gtgtcgccgg cggacttcat ccccttggcg gaagaaaccg gactgatcct tccgctgggt
      901 caatgggtgc tggaaaccgc ctgccatcag ctggcggcct gggcgaagct gcccgcaatg
      961 gcccatctga gcgtggcggt aaacgtcagt ccccgccagt tccaccagcc ggatttcgca
     1021 gatcaggtgt tggcaaccct ggagcgcacc ggcgccagtc cccatcgcct caagctggaa
     1081 ttgaccgagg gcatgctggt caccaacgtg gaagacgtca tcgcccggat ggttcggctc
     1141 aagagcatcg gcgtgggctt ttccctggac gactttggca tgggctattc ctcgctgtcc
     1201 tacctcaagc gtctgccgct ggaccagttg aagattgacc agagttttgt gcgggacatc
     1261 ctgatggacc cgaacgacgc ggccatctcg aaaatggtca tcgtcctggc tgaaagcctg
     1321 gggctggcgg tgattgccga gggagtggaa accgccgagc agcgcgacta tctggcgggg
     1381 cagggttgtt atacctacca ggggtatcta ttcagccggc cccttcctgc ggtagagttg
     1441 gagtcgttca ttcaatccgg tgtgacattg caggtatgcg taccaacgac gatctttcca
     1501 gttccggctt tgcgcggcta gttgccgaca agacgcccat ggtggagaaa atcgcccgca
     1561 atttgtcgcg caagctgccc accagcgtgg agcgggacga tctggtgcag gacggcatgg
     1621 tgggactcat cgacgcaatc ctgcgctgga cccgcgagac caccagcagc cactttgaca
     1681 actatgtcgc acagcgtgcg cagggcgcca tgctcgacgg actgcgcgcc aacgaccctg
     1741 gcagccgcag tctgcgccgg gccatgcggg ccgcagagca ggtgatccag cggctgggac
     1801 accagaaggg gcggcctccg ctggagtcgg aagtggcaca ggcactgggg ctggatattt
     1861 ccgaatacca gcgcatgctg caggatgtgc agggctattc gctcatctcg ctggaggacc
     1921 tgggaggcag cgacaacgcc cagccctatc tggactactg cgcggcgttc aacatcgacc
     1981 ccctgcgggt gctggagcgg tctgccttgc gccaggcctt gctggatgcc atacaggacc
     2041 tgcctgacca gaaaaagcag gtactgcggc tgtactacga gggcgagctc aagatgcacg
     2101 agatcggcag tgcgctgggc gtcagcgagg cacgggtgtc gcaactgcat tcgcacacca
     2161 ttgcgcagtt gcgcgcggtg attctgggtg gcgaacccag cccgcccctg ctcaagccgc
     2221 gcacccgcaa gcgggtggat gcttccgtcg ccggatgact tccatgtccg gtctctccat
     2281 cacaccagcc gtgtcggccg ggcgtgcgag tgagattgca cagagcttcg acctgcggga
     2341 tctgccggcc gacttctacg ccaatccgta ccctgtatat gccgcgttgc gcgaaagcgc
     2401 gcccgttcgg cagatgcccg atggctcctg cttcctgacg cactacgccg acctggtggc
     2461 ggtataccgc gatgcccaga cgttctgctc ggacaagaag ctggagtttg cacccaagta
     2521 caacgtacca ccgtacgacg cggcccaccc cgcgccgctg tttgtgcacc acaccaccag
     2581 cctggtattc aacgacccac cgctgcacac ccgtgtgcgc aagctcatca tgggcgcgct
     2641 cacccgccgc gccattgccg aaatggagcc gggtctggtc acgctggtgg atgcgctgct
     2701 ggaccggatg gagacccagg gcggcggtga cctgatcgag gactttgcgt cggccatacc
     2761 ggtggaaatc attggcaacc tgctgggcgt tccccatgcc gaccgcgctc ccttgcgcgg
     2821 ctggtcgctg gcggtgctgg gggcgctcga gccgcgcctg agccccgagc agcaggctgc
     2881 aggcaaccag gccgtgctgg atatgctggc ctacctgcaa ggtctggtgg ccgagcgacg
     2941 gcgccatccg ggagacccgg agcacgatgt gctcacgcgc ctgatccagg gcgaatccag
     3001 tggtgagcag ctcagcgaag tggaactgct gcagaactgc atctttattc tcaacgcggg
     3061 ccacgagacc acgaccaacc tgattggcaa cgcgctggtg gcattgcagg aacacccgca
     3121 agccaaagcg ggtttgctat caaatatgca gcggcttgcg caggatccac cggggctgga
     3181 ggcctgtttg acacttgctg tcgatgaatt cctgcggttt gagtcgtcca accagctggg
     3241 taaccggcgc gcgctgcgga cctgcgaagt gggtggggtg gcattgcctg agggggcgct
     3301 ggtgacgctg tgcattggcg cggccaaccg cgacccgctg cagtttgaac acccggacca
     3361 gctcgttctg gcacgccaga acaaccgcca cctggcattt ggctttggta tccaccagtg
     3421 cgcaggcctg agcctggcgc ggctggaagg gcgcattgcg atcggccgct tcctgcagcg
     3481 cttcccgcgc tacgcactga ctgccccgcc ggtgcggggt gggagagcgc gctttagggg
     3541 attcctgagc gcgccctttg ctactgacga ttcggtttag cttccgatca cgccgccgtc
     3601 ggctttggtg atcacgatgg tggcagagcg gggacgcttg ccagcgccat aacctgcgtt
     3661 cgcaggccac tggctggtgt acttcgacgc gtcgccaatg tccgccatct tggtggtttc
     3721 accgggatgc tggatgttga tgaacatcgc cttgccgtcc ggtgtttcgc acaggccagt
     3781 gatttcacag tccttggggc ctaccaggaa gcgcttcagg gtgtcctcgg tgggggcctt
     3841 gcccacaaag gtgtcgacgc gcttctcact gccatccgtg cgcttgtacg ccagggtttt
     3901 cttgccacca tcgcccacct ggcccggaat ggccgccagc atcatgcagt tggacttgtc
     3961 ggtgtaggca ccatcgtcgg tctggatcca gcacagcccc gtggcttcgc tgaacaccag
     4021 gccgtcgggc gatgacaggt cgttggtatc ggtgaggttg gagaggttga tgcgggattt
     4081 gtcggcatcg gactcggcgc caaacacata gatgtcccac ttgaaggaag tgtctgccgg
     4141 gttcgcctgg gccacgcgaa tgatgtggcc gtgcacattg cccttgcttt ccttgctgcc
     4201 cttcatgtcg ctgtagtagc gtgggttggc cgcgtccagt ccggcgtcgc cgtcgaccgt
     4261 gcggttgctg ttgttggtca gcgtcatgta gaactcgccg ttgcgcgggt tgatgccgcc
     4321 ccactctgga cgatccatct tggtggcgct cacggcgtct gctgccaggc gggtgaagat
     4381 ggctacgtcg gcatcgctct tgaattcaaa gtcggggttg gcggcaatga tggggttgtt
     4441 catcgacaac tcaatccatt cgcccgtgcc gttgtccttg aagctggcca cgtacagcgt
     4501 gcctttgtcg aggtacttgt cgccggtggc aagacggttg gctgggttgg catctgcggc
     4561 atcccacttg gcggcagaca caaacttgta catgtactca ttgcgtgcat cgtcgcccat
     4621 gtaggccacc acgggctgac cggctaccgg cttggcaaag ctcatgctct cgtgggcaaa
     4681 gcggcccaga cccgtgcgct tgcgcacggc ctggcgggag tcgtaggcat ccatttccac
     4741 cacgtagccg aaggtgttca tctcgttgcg gtagtcggcg gaggcgctgg cacccagcgc
     4801 accattgttc cagcgcacga aacggtcatc gctgcccgcg ctctcccagc cgtggcggct
     4861 ggccgcaccg gccttgcggc catagcggtt caaggctgca acctgcttgt cctttttgcc
     4921 acggcgttcg tcgtccttgg catcgcggaa gaagtagccg taccagtttt cctcacccga
     4981 aacaaaggtg ccccaggggg tcttgccggt gccgcagttg ttcagtgtgc cgcgggcgcg
     5041 tgtcgcgtca gcgctgaact tggtcaccag gtgctcactg ccacgggccg ggccgtggat
     5101 cagcacatcg gtcatggtgg tgacacggcg gttgaatgcg gatccggcat tgaaactcca
     5161 ctgaccgttc tgggcggaga cttccaccac ggacaggcca tggatagcca gctccttgtc
     5221 cacttcagcg gcagggcgtg gcagggtgga ggcgccaccg ttggcgtgga tgaagaacga
     5281 actcagtttc tcgtcggtgg tggcctcgtg gttgatagcc agcagggcgc gggtggtggc
     5341 gttcagcgcg ggcttgccct gggcatccag tccgaaatat tccatgccgt cgtggtggtc
     5401 gccggcgcgg ctttcccagt cggtgtcagt gccgtcattc ttgaacgctg gagtggttgc
     5461 gcgcagtgga tcacccagcg cgtaaatgac ttgcacgttg tatccggcag gcacaacgac
     5521 cttgtcggcc aggcttttgc cgactgctgc aaagcccatg gaacgcagtg gggcagacag
     5581 ggtgtttgcg ccgttgccag caccgccagc ggttgcgcag ccggtcaatg cagcgctgcc
     5641 aagcaccgtg gttgcggctg cactggcgga acccaggagc acgttgcgcc ggctcaggcg
     5701 ggcagccagg acggcgtcaa agtggggatt ggccgtggtg ttggagtctt cgttgttgaa
     5761 gatgtcagaa ggagagcggc tggcgttcga catgcaaatg acctcgtgag tgtaagaacc
     5821 cgagaatcat ggcgtcgctg tgtgacgaag ttgtgacaaa gccgggacgc aaccgtgtca
     5881 ccccgctggg catggcgggc tacacgctat cgctggcagt ctcttcaaag cagtcgtgga
     5941 ccgtgaaata gagcgacgtg aaaaacatcg ctgccatcag catggccagc ggaaacatca
     6001 tgacactgac cacctccaca ctgcccagca gtgaagccac gagcgacacc gccgtgccca
     6061 tgccgacaaa gacgcccatc caggccagac cgaacaaggt gaacgcccag aagttgcgca
     6121 ggcaggccac caggctgaag aacaggcttt tcaccggctg cacaccatgc cagtgcacca
     6181 gggctggcgc gtgccagaac agcagtgaca acggcaggta caaagccatc gatagcagta
     6241 ccgccagctg gaagtccggg gcctgcagca gttcgaccga catggcgcca cccaccaggt
     6301 agagctgcgc gaacttgccg ccatcggcca gggcagagat gcccagtacc agcaggaagc
     6361 ccgccgcata gatggcgccc agcagcagca tggcctgcaa ctgctgcttt ccggcccgaa
     6421 atgcgctgac cagcagcacc ggcatgggga actttccggt gagcgcttcc ttggtcgccg
     6481 ccatcaggcc cagcgtggcg ccgggcagga tcaccagtgc caggatgttg cccagcagcg
     6541 gaaccatgct gagcagggac atggccgcca tgaacatgaa gaacaggcca gacagcgcca
     6601 gcggctggcg ccagaacgtg cgcatgccct gtttgaccca ctgcacaccc tggccggccg
     6661 gaacaatctg gagtttcatg gaaggaccaa gggttggtgg atgcgctggc gcaggacgcg
     6721 ctcgaaatgg gttgggtcgt gggcctggag catcgaggcc tcacgcggca ggtgaaaatc
     6781 ccacagacgc gacagccaga agcgcaaggc gccggcccgc gccatggcgg gcagcagcgt
     6841 gcgttcctgg gcctgtagcg ggcgcacctg gccataggca tccagaaagg catcggtgcg
     6901 ctctgcgtct gccgtgccgc tggcaaggtc cacgcaccag tcgttcaggc acacggcaat
     6961 gtcaaacagc caggtgtcca caccggcaaa gtagaagtcg aagaaaccgg tcagctccgg
     7021 tgcctggtct ggcgccgtgg gcaccgcaaa catgacgtta tcgcggaaca ggtcggcgtg
     7081 gatgggcccg cgtggcaggg cgccgtaggc ggacagggtg gccacaaggt tctggtaggc
     7141 cagctcgctc tggagcaggg cggattgctc gggggcgaga aaaggcaaca ccacgggcac
     7201 ggtttcgttc caccagggca gtccgcgcag gttgggctgc tgcatctcga aactgcgccc
     7261 ggccaggtgc atgcgcgcca gcatgtcgcc taccgcggca cagtgggccg ccgagggcgc
     7321 cagttggctc ttgccccgca ggcgattaac cacggcggcc ggtttgccac ccagtctgtg
     7381 cagtacatcg ccgtcacggt tggcagccgg gtcaggtact ggaatgccgt gcaccgccag
     7441 atgcttcatc aggcgcaggt aaaacggcaa ctgctccgcc gtcagccgct cgaacagggt
     7501 cagcacgtag gctgcctggt cggtggtaac gaagtaattg gtgttctcga tgccgccgga
     7561 acaaccctcc atggactgga gggttcccaa cttgagcgaa gctaaaaatg tgctggcctc
     7621 gtcgaaggag acctctgtat aaaccgccat gggccaattg taggggggca ggtggtcggg
     7681 cctgccggcg cgcaggcaaa atgcccccat gagtgaaacc cccaacacac cacgccagac
     7741 cctgttgctg gtcgatggtt ccagctacct gtaccgcgcc ttctttgcgg gcggcgactc
     7801 catgagcatc acgctgcccg acggcacggt gcagaagacc ggcgccattc gcatcatgat
     7861 caacatgatg aacagcctgc gcaaggaata tccggctgcc tacgtggcct gcgtgttcga
     7921 tgccaaaggc ccgacgttcc gggacgaaat ctacccgcag tacaaggcgc accgcgaccc
     7981 catgcccgat gacctgcgca gccagatcgc accgatccat gaagtggtac gccacctggg
     8041 ctggaaggta ctggatgttc cgggggtgga agcggatgac gtgattggca ccctggcggt
     8101 agcggcggcc agccagggca ttgaagtcat catcagcagc ggcgacaagg acctggccca
     8161 gctggtcaat gaacacatca ccatcatcga caccatgaac ggcaagcgcc gtgatctggc
     8221 cggggtggaa gccgaatttg gcgtgcctgc gcggctgatg ctggactacc agacgctggt
     8281 gggcgatacc gtggacaacg tgcccggcgt acccaaggta ggccccaaga ccgccgtcaa
     8341 gtggttgcag gaatatgggt ctctgcaggg cgtggtggac aacgcggcca atatcaaggg
     8401 tgtggtgggt gagaacctgc gcaatgcact ggactggttg cccacgggcc gcacgctgct
     8461 gaccatcaag accgactgtg atctgaacgg ctgggtgccc ggtttgcctg ctatggattc
     8521 catagctgtg agcgcacagg atacgggggc tttggccact ttttatgaaa aatatgggtt
     8581 caaggggctg gccaagtctg tggccgccgc gtctaccccc gttcgccctg agcccgtcga
     8641 cgggcagacg cggcaaggcg agcgcggtct gttcgatgag cccgaagccg ccacccctgc
     8701 accccgtgtc agtgacctgg tgcacgacac catattgacc tgggagctgt tcgatcgctg
     8761 gttcgccaag ctgcaggccg ccgaactggt ggcactggac acagagacca cgtcgctgga
     8821 cgagatgcgc gccgaaatcg tgggcatttc cttcagcatg gaaccgggct cggctgccta
     8881 catcccggtg gcgcaccgtt accccggtgc gcccgagcag ttgaaccgcg aagaggtact
     8941 ggccaggctt aagccctggc tggaaaaccc ggccgccaaa aagctgggcc agcacatcaa
     9001 gtacgaccgc catgtgtttg ccaaccacgg catcgaagtg cagggctacg cccacgacac
     9061 catgctgcaa agctatgtgc tggaagtcca catgccccat ggtttgagta gcctggccga
     9121 acgccacctg gggcgcagcg gtatcacctt tgaagacctg tgcggcaagg gcgccagcca
     9181 gatctgtttt gaccaggtgg acatcgccaa ggcggccgaa tacgccgccg aagactcgga
     9241 ccagacgctg gatgtgcacc gcgtgctgtg gccccagttg caggccaatg acaagctgaa
     9301 gttcatttac gaactagaga tcgccagcag tgaaaccctg taccggatcg agcgcaacgg
     9361 cgtgctgatc gatgcgccca ccctggccaa acaaagccat gagctcggtc agcgcatcct
     9421 gcagctggag accgaggcct acgagattgc gggccagccg ttcaacttga gcagccccaa
     9481 acaactcggc gaaatcttct tcgacaagct gggcatgccc gtggtcaaga agaccgccac
     9541 cggtgcccgc agcaccgacg aagaggtgct ggaaaaactg gccgaagatt acccgctgcc
     9601 cgccaagctg ctggagcacc ggggtctggc caaactcaag ggcacctaca ccgacaagct
     9661 ggcccagctg gcgctgccgc gcactggccg cgtgcacacg cactacgcgc aggcggtggc
     9721 ggtgaccggg cgcctctcca gcaacgaccc caatctgcaa aatattccga tccgcactgc
     9781 ggaaggccgg cgcgtgcgcg aggcctttgt ggcgcctgcg ggcagcgtga tcgccagcgc
     9841 cgattacagc cagatcgagc tgcgcatcat ggcccacatc agcgacgacc cggcgctgct
     9901 gcgcgctttc catgcgggcc tggatgtgca ccgcgccact gccgccgaag tgtttggcgt
     9961 ggccgtggac caggtgtcca gcgagcagcg ccgctacgcc aaggtcatca acttcggcct
    10021 gatctacggc atgagcgcct tcggtctggc gcgcaatctg ggtatcgaca acaccgcggc
    10081 caagaactac atcgcccgct actttgagcg ttatccgggc gtcaagacct acatggaaac
    10141 cacacgggag cgggccaaac agcagggcta tgtggaaacc gtgttcggcc gccggctcca
    10201 gcttgcgggc atccagactg ccaagggtgc gcagctggcg gggctggaac gcgccgccat
    10261 caacgccccc atgcagggga ctgcagccga cctgatcaag ctcagcatga accgggtgca
    10321 gcaggtgctc gacgccgaac agcgcggtac caagatgatc atgcaggtgc atgacgaact
    10381 ggtgttcgag gtgccggaaa ccgaggtgga gtgggtcagg accgagatcc cgcgcctgat
    10441 ggccggggtc gccgaactca aggtgccgct gctggctgaa gtgggtgtcg ggccgaactg
    10501 ggacaaggcg cactgaaccc ggtagcttgc caccaggtat ggggaaccgt tctgaaatcc
    10561 ccctgtattg agcggatatt tggctttctg gctacactgc ctcgcgatcc agaaaacaac
    10621 ccggagacaa ccatgcacac caatatgaaa accgaatttg acgccctgtt gcctgctggc
    10681 acatccaccg agcagggtgc cacacggcgc actgcgctca aggccgcact gggcgtgggc
    10741 tacgccgcca ctgccatgcc tatcatggct cagaccgcca tcaaaacacc gtcggctggc
    10801 ctggccacgg gggagggcat ctacgatgtg gatggcttta tggtgccat
//
LOCUS       DAOSJM010000029        10706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6057_length_10706_cov_4.476387, whole genome shotgun sequence.
ACCESSION   DAOSJM010000029 DAOSJM010000000
VERSION     DAOSJM010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6057_length_10706_cov_4.476387"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /locus_tag="PKX82_01945"
     CDS             <1..1135
                     /locus_tag="PKX82_01945"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOF51036.1"
                     /translation="NSNQMLLEAMGLHVPGTAFINPGEDVRQELTREAVRTVLGITKA
                     KRFAPIGRIVDERCIVNAMVALLATGGSTNHLIHWVAVARAAGIVIDWDDFSALSDVV
                     PLLTRVYPNGSADVNQFQAAGGPGYVICELLDAGYLHADVLTVRAGGLREFTQIPSAD
                     SAGGLQWRDAGASRDESVVRPVSAPFSASGGLKLLQGGLGRSVIKISAVPEDRHVIEA
                     PCRVFDSQEALHEAFQANELNRDIVCVVRWQGPQANGMPELHKLTPPLAVLQGKGFRV
                     ALVTDGRMSGASGKVPAAIHVSPEAAAGGPLAKVRSGDLIRLDANAGTLAVLVDAALW
                     QSRTADAMPEALRAANGVGMGRELFANFRRNASTAEEGACSWF"
     gene            1150..1791
                     /gene="eda"
                     /locus_tag="PKX82_01950"
     CDS             1150..1791
                     /gene="eda"
                     /locus_tag="PKX82_01950"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOF51037.1"
                     /translation="MSTTPTLTALQVMQDAPVIPVIVLNDVAHAVPMARALVAGGVRM
                     LEVTLRTPQALACMEAIAKEVPEAVVGAGTVRTKADAQAAAMAGARFAVSPGYTSAVG
                     EACRDNGLALLPGVATGSEIMMAQQDGYTELKFFPAMQAGGPAMLKAWSGPFFDVKFC
                     PTGGVTLQNASEFLALPNVVCVGGSWLVPADAMARGEWSRITQLARDTLALRK"
     gene            complement(2073..2486)
                     /gene="gloA"
                     /locus_tag="PKX82_01955"
     CDS             complement(2073..2486)
                     /gene="gloA"
                     /locus_tag="PKX82_01955"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HOF51038.1"
                     /translation="MRFLHTMLRVGNLQRSIDFYTQVLGMHVLRTSENPEYKYSLAFL
                     GFDGGNPGQAEIELTYNWGVESYELGTAYGHIALGVPDAYAACAKIKAAGGNVTREAG
                     PVKGGTTVIAFVTDPDGYKIELIQRADDSAGAGLR"
     gene            complement(2519..3094)
                     /locus_tag="PKX82_01960"
     CDS             complement(2519..3094)
                     /locus_tag="PKX82_01960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOF51039.1"
                     /translation="MNATTNHATLDAWLAEEAAVVARLNAGPGPGVARPDQVTGLTGL
                     ELMQAMLRGDLPYAAIAKTLDFTIVEVSEGRAVFQGTPGPAHRNPMGTIHGGWYATLL
                     DSALGCAVHTMMPVGRGYTTAELGVNLVRAINPAKAPRVRVEGKVIHCGRQLATAEAR
                     LYGPDGTLYAHGTTTCLVFDMHHKPAGGATT"
     gene            3150..3632
                     /locus_tag="PKX82_01965"
     CDS             3150..3632
                     /locus_tag="PKX82_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOF51040.1"
                     /translation="MKTLVKPAVVADVPKPQGCTNLKLRQLMRRVAQHYDAELAKMGL
                     KTTQYSLLSHVVKLGPIRAVDLAGVIRMSTSTLSRNLQPLIASGWIEVNAGDDARSRL
                     ISATPAGQAKRTEAQRKWRIAQEGINQALGPERVVVLHALIDESLELLSPGSEEEVES
                     "
     gene            3683..4066
                     /locus_tag="PKX82_01970"
     CDS             3683..4066
                     /locus_tag="PKX82_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51041.1"
                     /translation="MTTFTKVILFTDADGRARFKEEALPLNQGTPQSMLSEVFASGGY
                     QLRTSPVGFRSQFHCTGAPQWCFILGGQMEIGLQGGVSRVFKPGEHFYSADVLPEGAT
                     FDSAVHGHWSRQVGSDPLVTLFVRG"
     gene            complement(4072..4770)
                     /locus_tag="PKX82_01975"
     CDS             complement(4072..4770)
                     /locus_tag="PKX82_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOF51042.1"
                     /translation="MVVATVDRAAAARVALVAGATGLVGQAVLAALLADKTYSAVHCV
                     GRRKLSLQHPKLVQHVVDFQAPSGLKALPHVDDCFIALGTTIKVAGSQAAFRAVDFEA
                     VVTVARAALAQGATKLGVVSAMGADASSAIFYNRVKGEMEQALQDMAFKTLVIARPSM
                     LAGNREALNQPSRAGERIGLALTQLLKPVIPANYRSIAAEDVARALTRTVKTAGTGTY
                     RLLSGEMQGAAARH"
     gene            complement(4664..6433)
                     /locus_tag="PKX82_01980"
     CDS             complement(4664..6433)
                     /locus_tag="PKX82_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF51043.1"
                     /translation="MNFEELNLAPAIVKAVREQGYETPTAIQAQAIPLVLEGHDLLGG
                     AQTGTGKTAAFTLPLLHRLSMSRSAQNKFGGTGIRALVLTPTRELAAQVEESVRTYGK
                     YLQLSSTVIFGGVGMNPQISKVKKGVDILVATPGRLLDLAQQGMLDLGQVQMLVLDEA
                     DRMLDMGFIHDVKKVLALVPKEKQSLLFSATFSDEIRELANGLLKNPQSVQVTPRNTT
                     VQRITQVIHPVGRGKKKALLAHIINEQNWSQVLVFTRTKFGANNVAEFLEKNGITAMA
                     LHGNKSQAARTQALAGFKSGEVRALVATDIAARGIDIDDLPHVVNYEIPNVSEDYVHR
                     IGRTGRAGADGAAVNLVCLDEEGFMQDIERFTKQKIEVKVVEGFGPEPGEKGEPIAMG
                     RQTIWGGAGKPPSRDVMQAAAKAARTEMLQRVRDNKAGQGGRAGGGGNGGGNGGGNRG
                     GQGQQRGNGGNGGGDRGDRAPREDRSGEFQPPRSHGARPPQGQGRGRGRGPGAGQPAG
                     FANEPRAPRAPAGQPDPMRTSVDMMAERGARRGNFGGGNRGGNGGGNGGGYGGSGGGR
                     SGGFGGGGNGSRGPGGSRGSFSR"
     gene            6738..8966
                     /locus_tag="PKX82_01985"
     CDS             6738..8966
                     /locus_tag="PKX82_01985"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOF51044.1"
                     /translation="MTKFMLRRLGVADDPGSLKPTLQGCIEAVLEQSGVLMEDVLSGL
                     KASLAPPKGKQQSLAPAQGPAREVIETLCSQAQSVTETFARELRAAVYGGDAQRRASQ
                     PLVRFDDFQFLEEDQIDANIEFALTQQEVMLAVDDVLPTLNAMISGLLGWVTVQAHLN
                     PLKPESFVHALRESIAQHIPDETSRGSVMTLAAGKMGVSLRQLYKQISDWLRSQGVEA
                     AGLPVTTVGGVGGPSKAVETTMSRTLLTLDKLRRLLSGELDPTPMVGGVKDFIHTVPA
                     SFDALQDMKMVEPMMKRLAARATLSEAAPGSPLLAATQQAIQRERTQSKNLGRQLGEE
                     VVRLMLENLMQDRRMLAPVRNNLKTLEPVLIQLSQSDPRFFSDRQHPARQLLDKLTHR
                     SLAFTTENEPGFVRFQKSFEAAVTVLAGSEGDATQFAAELRKLEEGWNRDEQAQRQRA
                     EEAARALLRAEQRNMLAQRLAEEFNARMEGKRVPDMVVDFLRGPWAQVVAEAQLKFAD
                     GSGDAGDYQALADDLIWSVQLKLARRNRTRLVQMVPVMLVKMRQGLHLISYPEERVAE
                     FFDALITFHEKAFETVRAVPSGTPIESVVDRVDASEMDASGLSESFWMAEDEASDSGF
                     MQDAGDISLDFTAPANTEEVIDPMEWSPESLMTGSWVDLALAGSWVRAQLTWASPHRT
                     LFMFISGAGMAHSMSRRTMQRLKGLGLIRLVSDGRVMDGALDAVAQVALRNDANRTVS
                     ED"
     gene            9042..10703
                     /gene="ettA"
                     /locus_tag="PKX82_01990"
     CDS             9042..10703
                     /gene="ettA"
                     /locus_tag="PKX82_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOF51045.1"
                     /translation="MAQYVFSMNRLGKIIPPKRFILKDISLSFFPGAKIGVLGLNGSG
                     KSTLLKIMAGVDKEFEGEAIPMAGLKIGYLPQEPQLDPTETVRQSVEGGMGESKAAQA
                     RLEEVYAAYAEEDADFDALAAEQAQLEAIIATAGDGGEHQLEIAADALRLPPWDAIIG
                     QLSGGEKRRVALCRLLLSKPDMLLLDEPTNHLDAESVDWLEQFLQRYSGTVVAITHDR
                     YFLDNAAEWILEMDRGSGIPYKGNYSDWLQQKQDRLVQEQKGEESRAKALKKELEWSR
                     QNPKARQAKSKARLARFEELSDFEYQKRNETQEIFIPVAERLGNEVIEFTNVSKSFGD
                     RLLIDNLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGTVKIGQTVKMAFVDQ
                     HRDELSSEKTVWEDVSGGLDILNVGKFQMASRAYCGRFNFNGGDQQKRVGTLSGGERG
                     RLHLAKTLIAGGNVLLLDEPSNDLDVETLRALEDALLEFAGSIMVISHDRWFLDRICT
                     HIMAAEGDSQWVFFDGNYQEYEADKKKRLGEEGAKPHRMRFKALK"
BASE COUNT         1951 a         3208 c         3495 g         2052 t
ORIGIN      
        1 caacagcaac cagatgctgc tcgaagccat gggcctgcat gtgcccggca ccgccttcat
       61 caaccccggc gaggatgtgc gccaggagct cacccgcgaa gccgtgcgta ccgtactggg
      121 catcaccaag gccaagcgtt ttgcgccgat tggccgcatc gtggacgagc gctgcattgt
      181 caacgcgatg gtggccttgc tggctaccgg tggctctacc aaccacctga tccactgggt
      241 ggccgtggcc cgcgcggcag gcattgtgat tgactgggat gacttctcgg cgctctccga
      301 tgtggtgccg ctgttgaccc gcgtgtaccc caatggcagc gcggatgtga accagttcca
      361 ggccgcgggc ggtccgggct atgtcatttg cgagctgctc gatgctggct acctgcatgc
      421 cgatgtgctg actgtgcgcg ccggtggttt gcgtgaattc acgcagatcc cttcggctga
      481 tagcgctggc ggtttgcagt ggcgtgatgc cggtgccagc cgcgatgaat cggtagtgcg
      541 gcccgtgtcc gcgccgttca gcgccagcgg tggcctcaag ctgttgcagg gtggtctggg
      601 ccgcagcgtg atcaagattt ccgcggtgcc ggaagatcgc catgtgattg aggccccgtg
      661 ccgcgtgttc gattcgcagg aggcgctgca cgaggcgttc caagccaatg aactcaaccg
      721 cgacatcgtg tgtgtggtgc gctggcaagg gccgcaagcc aatggcatgc ccgagctgca
      781 caagctcacg ccgccgctgg ccgtgctgca gggcaagggc ttccgcgtgg cgctggtgac
      841 ggacgggcgc atgagcgggg cgtcgggcaa agtgcctgcc gccatccacg tctcgcccga
      901 agcggcagcg ggcggaccgc tggccaaggt gcgcagcggt gacctgatcc gcctggacgc
      961 caacgccggt acgctggccg tgctggtgga tgccgccctc tggcagtccc gcacggcgga
     1021 cgccatgcct gaggcactgc gtgccgctaa cggtgtgggc atggggcgtg agctgtttgc
     1081 caatttccgc cgcaatgcct cgaccgccga agaaggcgcc tgcagctggt tttgattgaa
     1141 ggaatcgcga tgtccacgac acccactctt actgccctgc aggtcatgca ggatgcgccg
     1201 gtcattccgg tgattgtgct caacgatgtg gcccacgcgg tgcccatggc gcgcgccctg
     1261 gtggctggcg gcgtgcgcat gctggaagta accttgcgca ccccccaggc cctggcctgc
     1321 atggaagcca ttgcaaagga agtgcctgaa gccgtggtcg gcgccggtac cgtgcgcacc
     1381 aaggccgatg cccaggccgc ggccatggcg ggcgcccgct ttgcggtcag ccccggctat
     1441 accagtgcgg tcggcgaggc gtgccgcgac aacggcctgg cgctgttgcc cggtgtggcc
     1501 accggcagcg aaatcatgat ggcgcaacag gacggctaca ccgagttgaa gttcttcccc
     1561 gccatgcagg ctggcggtcc ggccatgctc aaggcctgga gtggcccgtt cttcgacgtg
     1621 aagttttgcc ccaccggcgg cgtgacgttg caaaacgcgt cggagtttct ggcattgccc
     1681 aacgtggtgt gcgtgggtgg ctcgtggctt gtgccggccg atgcaatggc caggggcgag
     1741 tggagcagga tcacgcagct ggcgagggat acgctggcgc tgcggaagta agctgccgtg
     1801 tcagagctaa gcgaaagagg gctactgccc gtgaatattg ggcaagcagc tataaattaa
     1861 atagcattct ggtttgacgc aaatccttac ctttctactc gccaacgatt tcattctggt
     1921 ggtcggcgat gtggaataca tgggcttgcg caaactcggc tttgctgcgt tctgaattgc
     1981 ggtaaatgtg tcagcctggt ggtgcattga actgatgcac tttggagctg ggtgaatcct
     2041 ttcaggcgcg aacaaagtcc atgagatgtg cctcagcgca agccagcacc tgcactgtcg
     2101 tctgcccgct ggatcagctc aatcttgtag ccatccgggt ccgtcacaaa cgcaatcacc
     2161 gtggtgccgc ctttgaccgg gccggcctcg cgtgtcacgt tgccgccagc ggccttgatc
     2221 tttgcgcagg cggcataggc atcgggcacg cccagggcga tgtggccgta ggcggtgccc
     2281 agctcgtagc tctctacgcc ccagttgtag gtcagctcga tctcggcctg gcctgggttg
     2341 ccgccgtcaa acccgaggaa ggcgaggctg tatttgtact cgggattttc ggaggtgcgc
     2401 agcacgtgca tgcccagcac ctgggtgtag aaatcgatgg agcgctgcag gttgcccacg
     2461 cgcagcatgg tgtggaggaa tcgcatggag gtctctcaag ccggtttgga agcgcacatt
     2521 atgtggtggc gccgccagcg ggcttgtggt gcatgtcgaa aaccaggcag gtcgtggtgc
     2581 catgggcgta cagggtgcca tcgggcccat agaggcgcgc ctcggcggtg gcgagctggc
     2641 ggccgcagtg gatgaccttg ccttccaccc gcacgcgggg tgctttagcg gggttgatgg
     2701 cccgcaccag attcacgccg agctcggcgg tggtgtagcc acggccgacc ggcatcatgg
     2761 tgtgcaccgc gcaacccagg gccgagtcga gcagcgtggc gtaccagccg ccatggatgg
     2821 tgcccatggg gttgcggtgc gcaggtcctg gagtgccctg gaatacggca cggccttcgc
     2881 tgacttccac gatggtgaag tccagcgtct tggcaatggc ggcgtagggc aggtcgccgc
     2941 gcagcatggc ctgcatcaac tccagacccg tcaggccggt cacctggtcg ggccgcgcaa
     3001 cgccagggcc ggggcctgca ttcaaacgtg cgacgacggc agcttcttcg gccagccagg
     3061 cgtccagggt ggcgtgattg gttgtggcgt tcatgggtgt tcctttgaat aaacttgcat
     3121 atgcaacaga tgtaactaca ataatcaaca tgaaaacact tgtcaagcca gcggtcgtcg
     3181 cggatgttcc caagccacag ggttgcacca acttgaagct gcgccagctc atgcgccgag
     3241 tggcccagca ctatgacgcg gagctggcca agatgggctt gaagaccacg cagtattcgc
     3301 tgctgtccca tgtggtgaag ctggggccga ttcgtgcggt ggatttggcg ggcgtgatac
     3361 gcatgagcac ctccacgctg tcgcgcaacc tgcagccgct gattgccagt ggctggattg
     3421 aagtgaacgc gggtgacgat gcgcgcagcc gcctgatcag cgctacgccg gccggccagg
     3481 ccaagcgcac cgaggcgcag cgcaaatggc gcattgcgca agagggcatc aaccaggcac
     3541 tgggacctga acgcgtggtg gtactgcacg cactgatcga tgagtcgctg gagctactgt
     3601 ccccgggctc cgaggaggag gtagagtcct gagtgcgctg cttggggaat aatgcccggc
     3661 attgttccca accgatcaca ccatgaccac cttcaccaaa gtcatcctgt tcaccgatgc
     3721 cgatggccgc gcccgcttca aggaagaagc gctgccactg aatcagggca cgccgcagtc
     3781 catgttgtcc gaggtgtttg catccggcgg ctaccagttg cggaccagtc cggtcggttt
     3841 tcgcagccag tttcattgca cgggtgcgcc gcaatggtgt ttcattctgg gtggacagat
     3901 ggagattggc ttgcagggag gcgtgtcacg ggtgttcaag cctggcgagc atttttactc
     3961 tgctgatgtc ttgcccgaag gcgccacctt tgattccgcc gttcacggcc actggagccg
     4021 tcaggtgggt tcggacccgc tggtcaccct gttcgtacgc gggtgaacag ttcagtgcct
     4081 ggcagctgcg ccctgcatct ctccggacaa tagccggtag gttcctgtgc cggcggtctt
     4141 gacggtgcgt gttagcgccc tggcgacatc ctctgccgca atggagcggt agttggccgg
     4201 aatcacgggt ttcagtagct gcgtcaaagc gagtccaatg cgctcgcccg cgcgggaagg
     4261 ctggttcagg gcttcacggt tgcctgccag catggagggg cgcgcaatca ccagcgtctt
     4321 gaacgccatg tcctgcaagg cctgctccat ttcgcccttg acgcggttgt agaaaatggc
     4381 cgaagaggcg tccgcgccca tggcgctgac tactcctaat ttcgtagcgc cctgcgcaag
     4441 cgctgcgcgg gctacggtca caacagcctc aaaatccacg gcgcggaacg cagcctggct
     4501 gccggccacc ttgatggtgg tgccaagggc aataaagcag tcgtccacat gcggcaacgc
     4561 tttcaggccc gatggggcct gaaaatccac cacatgctgg accagtttgg ggtgctgcag
     4621 ggacagcttg cgccgcccca cacaatgcac cgcggagtag gtcttatcgg ctaaaagagc
     4681 cgcgagaacc gcctggccca cgagacccgt tgcccccgcc accaaagcca cccgagcggc
     4741 cgccgcccga tccaccgtag ccaccaccat tgccgcctcc gtttccaccg cggtttccac
     4801 cgccaaagtt gccacggcgt gcgccgcgtt cagccatcat atcgacgctg gtacgcattg
     4861 ggtcgggctg accagcgggt gcacggggtg cgcggggttc attggcaaag ccagcaggct
     4921 gacctgcacc gggaccacgt ccacgtccac gcccttggcc ttggggtggg cgggcgccgt
     4981 gtgaacgggg aggctggaat tcgccgctac ggtcttcacg gggagcacga tcaccgcggt
     5041 caccgccgcc gttgccaccg ttaccgcgct gttggccctg gccaccacgg ttgccaccgc
     5101 catttccacc gccgttaccg ccaccacccg cacggccacc ctggccggcc ttgttgtcac
     5161 gcacacgttg cagcatttcg gtacgcgctg ccttggcagc cgcctgcatc acatcgcggc
     5221 tgggcggctt gccagcaccg ccccagatgg tctggcggcc catggcgatg ggttcgccct
     5281 tttcgcctgg ctcggggcca aaaccctcga caaccttgac ttcaatcttt tgcttggtga
     5341 agcgctcgat gtcctgcatg aagccttctt cgtccaggca caccaggttc actgcagcgc
     5401 catcggcacc cgcgcggccg gtacggccaa tgcggtgcac ataatcttcg ctgacgttgg
     5461 ggatttcata gttcaccacg tgcggcagat cgtcaatgtc gataccacgg gcggcaatgt
     5521 cggtggccac cagtgcgcgc acttcgccac ttttgaagcc agccagtgcc tgggtacggg
     5581 cggcctggct cttgttgcca tgcagggcca tggccgtgat gccgttcttt tccagaaact
     5641 cggccacgtt gttggcgccg aacttggtgc gtgtgaacac cagcacctgg ctccagtttt
     5701 gctcgttgat gatgtgggcc agcagggcct ttttcttgcc gcggcccacc gggtggatca
     5761 cctgggtgat gcgctgtacc gtggtgttgc gcggcgtgac ttgcacgctt tgcgggttct
     5821 tcagcaggcc attggccagc tcgcggattt cgtcactgaa ggtggcggag aacagcaggc
     5881 tttgcttttc cttgggcacc agggccaata ccttcttcac gtcatggatg aagcccatgt
     5941 cgagcatgcg gtcggcttcg tcgagcacca gcatttgcac ctggccgagg tccagcatgc
     6001 cttgttgggc caggtccagc agacggccgg gggtggccac cagaatgtcc acgccttttt
     6061 tgaccttgga gatttgcggg ttcatgccca caccgccaaa gatgacggtg gagctgagtt
     6121 gcaggtactt gccgtaagtg cggacagact cttccacctg ggcggcgagt tcacgggtgg
     6181 gggtcaacac cagggcacgg atgccggtgc cgccaaattt gttttgcgcg ctgcggctca
     6241 tggagaggcg gtgcagcagg ggcagggtga aggctgcagt cttgccggta ccggtctggg
     6301 cgccgcccag caggtcatgg ccttccagca ccagcggaat ggcctgggcc tggatcgcgg
     6361 tgggagtctc gtaaccctgc tcgcgcacgg ctttcacaat ggccggggcc agattcaatt
     6421 cttcgaagtt catagttgaa ggcgcccatc ctgggcgctt ggataccggc cgttctgcgc
     6481 gcggtgtggc gcacagtcag tcaatggcag atggattcaa aagtgggacg caattgtcgg
     6541 ttgggtcccc agcaaggtgc tggcatcgcg ggcaactgaa gcgccgcaac aggcagtatt
     6601 gtctcacgtt cgcgacattg gtcgggaagc ggagccagaa acagctgatt tcgcggagaa
     6661 aacggctgta atagagtgta agcaggaggg tgtagcaact ggttttgtgg ctctgcacct
     6721 gccaaaatac aaagacaatg accaaattca tgttgcgccg cctgggagtg gccgatgacc
     6781 ccggaagcct gaagccgacc ttgcagggat gcatcgaggc cgtgctggag caaagcggcg
     6841 tgctgatgga ggatgtgttg tcgggcctga aggcatcgct ggctcctccc aagggcaagc
     6901 agcagtctct ggccccggcg caaggacccg ccagagaggt tattgaaacg ctgtgttccc
     6961 aggcgcagtc cgtgacggag acgtttgcca gagagttgcg cgcggcagtc tacggcgggg
     7021 atgcgcagcg tcgggcgtcg cagccgctgg tgcgctttga tgatttccag tttctggaag
     7081 aagaccagat cgatgccaac atcgagttcg cgctgacgca gcaggaagtc atgctggcag
     7141 tggacgacgt attgcccacc ctcaatgcca tgatcagcgg ccttctgggc tgggtcactg
     7201 tgcaggcaca cctcaatccg ctcaagccgg agtcgtttgt gcatgccctg cgcgaaagca
     7261 tcgcacagca cattccggac gagacctccc gtggcagtgt catgaccctg gccgcaggga
     7321 agatgggtgt gagtctgcgc cagttgtaca agcagatttc cgattggctc cggtcccagg
     7381 gggtggaggc cgcaggcctg ccggtcacca cggtgggcgg agtgggtggg ccatccaagg
     7441 cggtggagac caccatgagc cgcactctgc tgacgttgga caagttgcgg cggctgctgt
     7501 cgggcgagct ggaccccact cccatggtgg ggggtgtcaa agatttcatc cacacggtcc
     7561 cggcgtcgtt tgatgcgctg caggacatga agatggtgga gccgatgatg aagcgtctgg
     7621 ctgcgcgcgc cacgctgtcg gaggcggccc cgggttcgcc cctgctggcg gcgactcagc
     7681 aggcgattca gcgggagcgg acccagagca aaaacctggg gcgccagctt ggtgaagagg
     7741 tggtgcgcct gatgctggag aacctgatgc aggaccgccg gatgctggca ccggtgcgca
     7801 acaatctcaa gacgctggag ccggtgctga tccagctgtc gcaatcggac ccgcgctttt
     7861 tcagcgaccg gcaacatccc gcccgccagt tgctggacaa gctgacccac cgtagcctgg
     7921 cgtttacgac cgaaaacgaa ccaggattcg tccgcttcca gaagtcgttt gaagcggctg
     7981 tgactgtgct ggcgggcagt gagggtgacg caacacaatt cgctgctgaa ttgcgcaagc
     8041 tcgaagaggg ctggaatcgg gatgagcagg cgcaacgcca gcgcgccgag gaggccgcgc
     8101 gtgcactgct gcgtgccgag caacgcaata tgctcgcgca gcgcctggca gaagagttca
     8161 atgcgcggat ggaaggcaag cgcgttcccg acatggtggt cgatttcctg cgtggcccct
     8221 gggcccaggt ggttgccgaa gcccagctga agtttgccga tggttccggg gatgctggcg
     8281 actaccaggc gctggcagac gacctgatct ggagcgtgca gctcaaactg gcccgacgca
     8341 accgtacccg gctggtccag atggtgccgg tcatgctggt caagatgcgc cagggcctgc
     8401 acctgatttc ctatccggaa gagcgggtgg ccgagttctt tgatgcgctg atcactttcc
     8461 atgaaaaggc gtttgagacc gtgcgtgcgg ttccgtcggg gacccccatc gaatcggtgg
     8521 tggaccgcgt cgatgcgtcg gaaatggatg ccagcggcct gtccgaatcg ttctggatgg
     8581 cagaggacga ggccagcgac tccggcttca tgcaggacgc cggggatatt tccctggatt
     8641 tcactgcacc tgcaaatacc gaagaagtca tcgacccgat ggagtggagt cccgagagcc
     8701 ttatgaccgg ctcctgggtg gacttggccc tggccggctc ctgggtgcgc gcgcaactga
     8761 cctgggccag cccgcaccgc accctgttca tgttcatctc cggtgctggc atggcgcact
     8821 ccatgtcccg ccggaccatg cagcggctca aagggctggg gctgatccgt ctggtatcgg
     8881 atggccgcgt gatggacggc gcgctggacg cggtggccca agtggccctg cgcaacgacg
     8941 caaatcggac ggtttcagag gactaattcg ggtccggggc gtcggcgtct ggctctgcag
     9001 tcgataatgg cggctttacg cttccaagaa tagctactgc aatggcccaa tacgtcttct
     9061 ccatgaaccg cctcggcaag atcattccgc cgaagcgttt cattctcaag gacatctccc
     9121 tgtccttttt ccccggcgcc aagattggtg tgctcggtct caacggttcc ggcaagtcca
     9181 cgctactcaa gatcatggca ggtgtcgaca aggagttcga gggtgaagcc attccgatgg
     9241 cgggcctgaa aatcggctac ctcccgcagg agccgcaact cgaccccacg gaaaccgtgc
     9301 gccaatccgt tgagggcggc atgggcgaat ccaaggccgc gcaggcccgc ctggaagagg
     9361 tgtacgcagc ttatgccgaa gaagacgccg acttcgacgc actcgctgca gagcaggccc
     9421 agctggaagc catcatcgcc actgccggtg atggcggcga gcaccagctg gaaattgctg
     9481 ccgatgcact gcgtctgccc ccatgggacg ccatcattgg ccagctctcg ggtggcgaga
     9541 agcgccgtgt ggcgctgtgc cgtctgctgc tgagcaagcc cgacatgctg ctgctcgacg
     9601 agcccaccaa ccacctggac gccgaatcgg tggactggct ggagcaattt ctccagcgct
     9661 actctggcac cgtggttgcc attacccacg accgctactt cctggacaac gcggccgagt
     9721 ggattctgga aatggaccgc ggctccggca ttccctacaa gggcaactac agcgactggc
     9781 tgcaacagaa gcaggaccgg ttggtgcagg agcaaaaggg cgaggagtcc cgcgccaagg
     9841 ccttgaagaa ggagctggaa tggtcgcgcc agaaccccaa agcacgccag gccaagagca
     9901 aggcccgtct ggcccgtttc gaggaactgt cggatttcga ataccagaag cgcaacgaaa
     9961 cgcaggaaat cttcattccc gtggccgagc gcctgggcaa tgaagtgatc gagttcacca
    10021 acgtcagcaa gtcctttggt gaccgtctgc tgatcgacaa cctgagcttc aaggtgcccg
    10081 caggtgccat cgtcggcatc attggcccca acggcgcggg taaatccacg ctgttcaaga
    10141 tgattgccgg caaggagcag ccggattcgg gcacggtcaa gattggccag actgtcaaga
    10201 tggcgtttgt cgaccagcac cgtgacgagt tgtccagcga gaagacggtc tgggaagacg
    10261 tctctggcgg gctcgacatc ctgaatgtcg gcaaattcca gatggccagc cgtgcctatt
    10321 gcggccgctt caacttcaat ggcggcgacc agcagaaacg cgtcggcacg ctgtccggtg
    10381 gtgaacgtgg tcgcctgcat ctggccaaga ccctgattgc cggcggcaac gtgctgctgc
    10441 tcgacgagcc atcgaacgac ctggacgtgg aaaccctgcg cgcgctggaa gacgccttgc
    10501 tggaatttgc gggttccatc atggtcatca gccacgatcg ctggttcctg gaccgcatct
    10561 gcacccacat catggccgcc gagggcgaca gtcagtgggt cttctttgat ggcaactacc
    10621 aggaatacga agccgacaag aagaagcgtc tgggggaaga aggcgccaag ccgcaccgca
    10681 tgcgtttcaa ggcactgaag taattt
//
LOCUS       DAOSJM010000030        10618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6119_length_10618_cov_5.078387, whole genome shotgun sequence.
ACCESSION   DAOSJM010000030 DAOSJM010000000
VERSION     DAOSJM010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6119_length_10618_cov_5.078387"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="PKX82_01995"
     CDS             complement(<1..739)
                     /locus_tag="PKX82_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOF51046.1"
                     /translation="MTDSLSTHALHDLSVHDMALALRNKQVSSVELAQHFLQRSRDHA
                     HLGAFVAIDETTTLAQAQAADRRIAQGSVGALEGVPIAHKDIFVTKDFATTAGSRMLK
                     GYRSPFDATVVAKLAADGAVTLGKLNCDEFAMGSSNENSAIAPVGQEAVTPVRNPWNA
                     ERIPGGSSGGSAAAVAARLAPAATGTDTGGSIRQPAAFCGITGIKPTYGRASRYGMVA
                     FASSLDQAGPMARSAQDCALLLSSLCGP"
     gene            complement(757..1056)
                     /gene="gatC"
                     /locus_tag="PKX82_02000"
     CDS             complement(757..1056)
                     /gene="gatC"
                     /locus_tag="PKX82_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOF51047.1"
                     /translation="MSLTSSDIARLANLARLELQPAESERILGQMNDFFSIVEAMRAV
                     DTSGLEPMAHPVAAIQDVALRLREDVVSEPNQREANQRSAPAVEHGLFLVPKVIE"
     gene            1260..2303
                     /locus_tag="PKX82_02005"
     CDS             1260..2303
                     /locus_tag="PKX82_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOF51048.1"
                     /translation="MFGAFRRYFSTDLAIDLGTANTLIVARGQGIVLNEPSVVAIRHE
                     GGPQGKKSIQAVGKEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVLPR
                     GILKPSPRIIICVPCGSTQVERRAIRESALGAGASDVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSVRVGGDKFDEAIINYIRRNYGMLIGEPTAEA
                     IKKQIGSAFPGSEVKEMEVRGRNLSEGVPRSFTISSNEILEALTDPLNNIVSAVKNAL
                     EQTPPELGADIADRGMMLTGGGALLRDLDRLLAEETGLPVLVAEDPLTCVVRGCGIAL
                     EQMERLGSIFTSE"
     gene            2328..3248
                     /gene="mreC"
                     /locus_tag="PKX82_02010"
     CDS             2328..3248
                     /gene="mreC"
                     /locus_tag="PKX82_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOF51049.1"
                     /translation="MPLGTLDRDPPPFFRQGPSALSKLAVCSALALFLMVADARFKVM
                     QPLRVGLAAVLYPVQWLAMQPIQIAQSAGQYFSSLRSAQVAKDDAFRKLGLQAQRANQ
                     VEQLALENTRLRNLLGLRERLQSGGMAAQVLYDAADPYTRKVVIDRGMVDQVRPGSPV
                     VDEHGVIGQVTRVYPLVSEVTLLTDRDHAIPVLNTRTGARGVAFGDTSTHADAMELRY
                     MAANADVAVGDLLTTSGVDGVYPPGLPVARVEKVERRVDTVFARIYCVPLGLVAGASH
                     VMVLEPVMAQIAERPAPELPVASPKKGVTK"
     gene            3277..3807
                     /gene="mreD"
                     /locus_tag="PKX82_02015"
     CDS             3277..3807
                     /gene="mreD"
                     /locus_tag="PKX82_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOF51050.1"
                     /translation="MIMRPGQQLLLPASPLFIWSSLVVAMLFNMVQNMGLWGRAAWVP
                     DLLAVVLVFWTVHQPLRIGIGASFIFGLLMDVQQGSLLGQHALAYTVLSFFAITVHRR
                     LLWFSVPSQAAQVFPLFFAAHAVELAVRLIGGGVFPGFWILLAPVLEAALWPITSVVL
                     LAPQRRAPNPDANRPL"
     gene            3820..5760
                     /gene="mrdA"
                     /locus_tag="PKX82_02020"
     CDS             3820..5760
                     /gene="mrdA"
                     /locus_tag="PKX82_02020"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOF51051.1"
                     /translation="MTELRNVEMDLARFRARVFVISLVVLTCFFLLAARLVYLQIWRH
                     EDLRAQAESNRTSIVPIVPNRGLIQDRNGIVLASNYSAYTLEITPSKSGGVERTIDEL
                     AQVVDITPRDRRRFKRLMEESKSFESVPIRTRLTDVEVARFAAQRFRFPGVEIKARLF
                     RNYPYGELASHVIGYIGRINRMEKEKIDDSEDQANYRGTEYIGKLGVEQNFESQLHGT
                     TGVERMETSAGGRAVRKLASTPSTPGNMVMLSIDIKLQKLVEDMYGDRRGALVAIDPR
                     NGEVIAFVSKPTFDPNLFVDGIDADSWAALNEDIDKPLLNRALRGTYPPGSTYKPLMA
                     IAALNSGKRAANVVIQDNLTYSFGGRTFGSPEADRPGPKDMRLAIVGSSNVYFYSLAN
                     EMGVDLIHDQLAPFSLGQRTGIDLQGEVTGDLPSQEWKRKRFRKPEQQKWYAGETISL
                     GIGQGYNNFTMLQMANAYATLASGGLRFKPRLVREIKDMVQHTSQRVASEALEPLPLN
                     PEHVNVIRNAMHGVTLEGTSAKVFAGAGYTSGGKTGTAQAVGLRAGEKYSSLKADEHK
                     RDHSLYVAFAPVENPTIALALIVENAGWGSTSAAPIARRVFDYWLLGQYPSEEDLAAV
                     RKGEATAPIGKPRTASEIPWPR"
     gene            complement(5778..6536)
                     /locus_tag="PKX82_02025"
     CDS             complement(5778..6536)
                     /locus_tag="PKX82_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOF51052.1"
                     /translation="MELLVVSLASLLAGFVDSIVGGGGLILVPALFAVFPQTHPATLF
                     GTNKGASVWGTGIATVQYSRRVTMPWHALGPAAVAGFAASLAGAWLVTVISPEFLRKV
                     LPGVLLLVLVYTLLKKELGRHHAPRFSGLQETLIAIGIGAVIGFYDGFFGPGTGSFFV
                     FLLVRLLGYDFLHASAGAKLLNTATNLAALVLFTLKGHVWWHFALVMAVANVVGSLAG
                     THLALRLGTGFVRGVFIAVVSVLILKTGYDAFLR"
     gene            complement(6759..8507)
                     /locus_tag="PKX82_02030"
     CDS             complement(6759..8507)
                     /locus_tag="PKX82_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOF51053.1"
                     /translation="MNDTTFDYIIVGAGTAGCLLANRLSSDATKRVLLIEAGRKDDYH
                     WIHIPVGYLHCIGNPRTDWLYNTEADPGLNGRSLKYPRGKTLGGSSSINGMIYMRGQA
                     RDYDQWAQLVGDANWTWDNNLPYFKLHEDHYKGASAMHGARGTAPALMQDASTPYRAI
                     LRHHNAGGEWRVEKQRLRWDVLDAFAQAATQAGIPATDDFNRGNNEGVGYFEVNQKEG
                     WRWNTAKAFLRPKCYGRPNFELWTSAQASRLVVEPSQGDDAQPLRCTGVQVWDGQEMV
                     TATARAEVILCAGAVGSPQLLQLSGLGPAALLQARGVSVVKDLPGVGENLQDHLQIRA
                     VFKVKHVPTLNTQANSLLGKAKIGLEYALKRSGPMSMAPSQLGAFTRSDASQPHPNLE
                     YHVQPLSLDAFGQPLHNFNAFTASVCNLNPTSRGSVQIKSADFKDAPAIAPNYLSTDA
                     DRKIAADSLRLTRSIVAQPALQKYEPEEYKPGSQFQSDADLARLAGDIATTIFHPVGT
                     TKMGRDDDPMAVVDARLKVRGVAGLRVVDAGVMPLITSGNTNSPTLMIAEKAAQWIAE
                     DGALGKHPTVITSAAL"
     gene            complement(8514..9308)
                     /locus_tag="PKX82_02035"
     CDS             complement(8514..9308)
                     /locus_tag="PKX82_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOF51054.1"
                     /translation="MNPSDSPFQSTPELAVEVRGPVLWLTITREERRNAMSHGVLAAM
                     AKSIAAAQSQRDIRAIVVTGAGSKAFCAGADLQSANAFTTDYSEPHGHLAQLLRVAKA
                     SYIPLIARVNGTCMAGGMGLMSMCDMAVASGHAVFGLPEVKVGVFPAQVLSVLQHLIP
                     RRVLVEMCITGEPITSAQALQYGLVNHVDDDVDAKLQWLLDRLLDKSPAAIRRGLYTM
                     KKVEAMSFEESMAFTESQIALFTLTDDAREGQKAFQEKRKPVWAGK"
     gene            9395..10522
                     /locus_tag="PKX82_02040"
     CDS             9395..10522
                     /locus_tag="PKX82_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51055.1"
                     /translation="MEDTQSRIAFETLPEGECAVALGIWTAAHLAVPEHWAALQSALA
                     QAATRGPAIGWDLRRLQRLDHSGAQVLWNAWGRHWPQRLELLDVQRAMLERIARFSVP
                     PPRPERSSPLDAMETLGDWVFSMGGHVAGLVRLLGQLVIDLLKLVRHPVKGPWRDVSG
                     HLYHIGATALPITALVGFLIGVVLAYLMSQQLRQFGADAFIVNILGISLIRELGPVLA
                     AILIAGRSGSAITAQIGVMRVTEELDAMRVMGIPKGYRLVMPRVIAMAVAMPLIAVWT
                     TIAALFGGMLAADLAIGLSPAYFVTALPKAVELSNLWLAVSKSVVFGVLIALIGCHYG
                     LRVQPNTESLGQGTTASVVTSITIVILVDALFAVVFKSVGI"
BASE COUNT         1938 a         3128 c         3434 g         2118 t
ORIGIN      
        1 cggggccgca caggctggag agcagcaatg cgcaatcctg tgcgctacgg gccatcggcc
       61 cggcctggtc caggctggaa gcgaaggcca ccatgccata gcgcgaggcg cgtccgtagg
      121 tgggtttgat gccggtaatg ccgcagaacg cagccggttg gcggatcgag ccaccggtat
      181 cggtgcccgt cgcagccggg gccagccggg ctgccacggc agccgcgctg ccacccgacg
      241 agccacctgg aatgcgctcg gcattccagg ggttgcgcac gggcgtgacg gcttcctgac
      301 caaccggcgc aatcgccgaa ttttcgttgc tggagcccat ggcgaattcg tcgcagttga
      361 gcttgcccag ggttacggca ccgtcggcgg ccagtttggc aacgacggtg gcatcgaacg
      421 gggagcggta gcccttgagc atgcgcgaac ccgcagtggt cgcgaagtcc ttggtgacaa
      481 agatgtcctt gtgcgcgatg ggcacacctt caagggcgcc gacagagccc tgcgcgatgc
      541 gccggtccgc tgcctgggcc tgcgccagtg tggtggtctc atcgatggca acaaacgcac
      601 ccaaatgggc gtggtcgcgg ctacgctgca gaaagtgctg ggccagctcg acggaggaca
      661 cctgtttgtt cctgagtgcg agtgccatat cgtgcacgct gaggtcgtgc agggcgtggg
      721 tggaaagaga gtctgtcatc gtgtgttctg cgtgtcttat tcaatgacct tggggaccag
      781 gaagagcccg tgctcgacgg ccggtgcact gcgctggttg gcctcgcgtt ggttgggctc
      841 ggacaccaca tcctcgcgca accgcagggc cacgtcctga atggctgcta ccgggtgtgc
      901 catgggctcc aggccggaag tgtccacggc acgcatggcc tccacgatgc tgaaaaaatc
      961 gttcatctgg cccaggatgc gctcgctttc agcgggttgc agctctaggc gggccagatt
     1021 ggcaaggcgc gcgatatcgg aagatgtcag tgacatggtg aaaaaagagt tgaaaccaaa
     1081 ggtaaatcgg acaatgcagg cccagacagg gtgggttatt cacagggcat cggttattat
     1141 cctagctttg gtgtagacgc agcgaaaccg ttgactttga cgccaaactt acaatttttg
     1201 caacgataaa acatggcgac gggccgccga agcgccgtcc gtgcccgaga ggatttgtga
     1261 tgtttggagc tttccgtcgg tacttttcca ccgaccttgc gattgacttg ggtaccgcca
     1321 ataccctgat tgtggcgcgt ggacagggca tcgtcctgaa tgaaccgtct gtggttgcca
     1381 ttcgccacga gggcggaccc cagggcaaaa agtccattca ggcggtgggt aaggaagcca
     1441 aggccatgct gggcaaagtg ccgggcaata ttgaggcgat ccgccccatg aaggacggcg
     1501 tgattgccga cttcacggtt accgagcaaa tgctcaagca gttcatcaaa atggtgctgc
     1561 cccgcggaat cctgaaaccc agccctcgga tcatcatttg cgtgccctgc ggttcgaccc
     1621 aggtcgagcg acgtgcgatt cgtgagtctg cgctgggcgc tggcgccagc gatgtgtacc
     1681 tgattgaaga acccatggca gcagccattg gtgccgggct gccggtgtcg gaagccagcg
     1741 gctccatggt ggtggacatt ggtggtggta ccaccgaagt cggtgtcatc tccctgggcg
     1801 gcatggtcta caaaggctct gtgcgcgtcg gtggtgacaa gttcgacgaa gccatcatca
     1861 actacatccg ccgcaactac ggcatgctga tcggtgagcc caccgcagaa gccatcaaga
     1921 aacagatcgg atccgctttc cccggctccg aagtcaagga aatggaagtg cgcggccgca
     1981 acctgtccga aggcgtcccc cgcagcttca ccatttcgtc caacgaaatt ctggaagcgt
     2041 tgaccgatcc gctgaacaac atcgtctcgg cggtcaagaa cgcgctggag caaacgcctc
     2101 ccgagttggg tgccgatatt gcggaccgcg gcatgatgct caccggtggt ggcgcgctgt
     2161 tgcgtgactt ggaccgcctg ctggccgaag aaaccggcct gccggtgctg gtggccgaag
     2221 acccactgac ctgtgtggta cgcggttgcg gcattgcgct ggaacagatg gagcgactgg
     2281 gttccatttt caccagcgaa taaagtcggt acagcgatgc ccgtaccatg ccattaggta
     2341 cgctggacag agatcctccc cccttcttcc gccagggccc ttcggccctg tccaagctgg
     2401 ccgtctgtag tgcgctggca ctgtttctca tggtggccga tgcccgtttc aaggtgatgc
     2461 agccgctgcg tgtggggctg gcggcggtgc tctacccggt gcagtggctt gccatgcagc
     2521 ccatccagat cgcgcaatct gcagggcagt acttttcttc actgcgttcg gcgcaggtgg
     2581 ccaaggatga tgccttccgt aaactgggct tgcaggcaca gcgtgccaac caggtggagc
     2641 agctggccct ggagaacaca cgtctgcgca acctgctggg cctgcgtgag cgactgcagt
     2701 cgggcggcat ggcggcgcag gtgctctatg acgcggccga cccctacacc cgcaaggtgg
     2761 tcatcgacag gggcatggtg gaccaggtgc gtcctggatc gccggtggtg gatgaacacg
     2821 gtgtgattgg ccaggtcacg cgcgtctatc cgttggtgag tgaagtcacg ctgttgaccg
     2881 accgggacca cgcgattccc gtactcaata cccgcaccgg cgcacgtggc gtggcatttg
     2941 gcgatacatc cacccatgcc gatgcgatgg agttgcgtta catggccgcc aatgccgacg
     3001 ttgccgtggg tgacctgctg accaccagtg gcgtggacgg tgtctacccg ccgggcttgc
     3061 cggtggcgcg tgtggagaaa gtggagcggc gggtggatac ggtttttgct cgcatttact
     3121 gcgtaccgct agggctcgtg gctggggcca gccatgtgat ggtgctggag cctgtcatgg
     3181 cacagatcgc ggagcgacct gcaccagaac tgccagtcgc aagtcccaaa aaaggggtaa
     3241 ctaaatgagc gcgtggtcat tgttggaggt gttgcaatga tcatgcgtcc cggccagcaa
     3301 ctcctgttgc ccgccagccc cctgtttatc tggagcagtc tggtggttgc catgttgttc
     3361 aatatggtgc agaacatggg cttgtggggg cgtgccgcat gggtgccgga tttgctggcc
     3421 gtcgtgctgg tgttctggac cgtgcaccag ccgctgcgca ttggtattgg cgcctcgttt
     3481 atctttggcc tgctgatgga tgtgcagcag ggcagcctgc tgggtcagca tgcactggct
     3541 tacaccgtgt tgagtttctt tgccatcacc gtccaccgcc gcctgctgtg gttttcggtg
     3601 ccgtcgcagg cggcgcaggt atttccgctt ttttttgcag cgcacgccgt cgagctcgcg
     3661 gtgcgcctga ttggcggcgg cgtgtttccc ggtttctgga tattgttggc gccggttctg
     3721 gaagccgcgt tgtggccgat caccagcgtt gtgctactgg ctccgcaaag acgcgccccg
     3781 aaccccgacg ccaaccgtcc cctctgaccc gggtctgcca tgacggaact gcgtaacgtt
     3841 gaaatggatc tggcacggtt tcgtgcgcgc gtgttcgtga tcagtctggt ggtgctcaca
     3901 tgcttcttcc tgctggcagc gcggctggtg tatctgcaga tctggcgaca cgaagacctg
     3961 cgtgcgcaag ccgagagcaa ccggacatcc attgttccca tcgttcccaa ccgggggctg
     4021 atccaggacc gcaacggcat cgtgctggcc agcaattact ccgcctacac cctggaaatc
     4081 acgccgtcca agagtggcgg tgtagagcga acaattgacg agctggcgca agtggtggac
     4141 atcacgcccc gtgaccggcg ccgtttcaag agactgatgg aggaatccaa gagctttgag
     4201 tcggtgccga tccgaacccg cctgactgat gtcgaagtcg cccgttttgc agcccagcgt
     4261 ttcaggtttc caggtgtcga aatcaaggcc aggctgtttc gcaactaccc ttatggagaa
     4321 ctggccagcc atgtgatcgg ctatatcggg cgcatcaatc ggatggaaaa ggaaaagatt
     4381 gatgacagcg aggaccaggc gaattaccgt ggcaccgagt acatcggcaa gctcggtgta
     4441 gagcagaact tcgagagtca gctgcatggc accactggcg tggagcgtat ggaaacgtct
     4501 gccggtggcc gcgcggtgcg caaactcgcc agcacgccct ccacgccggg gaacatggtg
     4561 atgctgtcca tcgacatcaa actgcaaaaa ctggtggagg acatgtacgg cgatcgccgg
     4621 ggggcgctgg tggcaatcga cccgcgcaat ggagaggtga ttgcctttgt atccaaaccc
     4681 actttcgacc ccaatctttt tgtggacgga atcgacgcag acagctgggc ggcgctgaat
     4741 gaagacatcg ataagccgct cctgaaccgc gcactgcgag gtacctaccc gccgggttcg
     4801 acctacaagc cactgatggc gatagcggca ctcaacagtg gcaagcgcgc ggccaacgtg
     4861 gtcattcagg acaacctgac ctacagcttc ggtggacgta cttttggcag ccccgaggcc
     4921 gaccgccccg gacccaagga tatgcgtctg gccatcgtcg gttcgtccaa cgtctacttc
     4981 tacagcctgg ccaacgaaat gggcgttgac ctgattcacg accagcttgc gcctttcagc
     5041 ctgggtcaac gcaccgggat tgatctgcag ggcgaggtga ctggcgactt gccgtcgcag
     5101 gaatggaagc gcaagaggtt tcgcaagccc gagcaacaga aatggtatgc cggcgaaacc
     5161 atctcgctgg ggatcggaca gggctacaac aatttcacca tgttgcagat ggccaatgcc
     5221 tacgcgaccc ttgcgtctgg cggtctgcgc ttcaagccac gtctggttcg tgagatcaag
     5281 gacatggtcc agcacaccag ccagcgggtg gccagcgaag cgctggagcc gttgccgctc
     5341 aatcctgagc atgtgaatgt catccgcaac gccatgcacg gagtaacgct ggagggcacg
     5401 tcggccaagg tttttgccgg cgccggctac accagtggcg ggaaaaccgg aaccgcccag
     5461 gccgtgggtc tgcgggctgg cgagaaatac agcagcctca aagccgacga gcacaagcgc
     5521 gaccattcct tgtacgtcgc ctttgcaccg gtagagaacc cgacgattgc actggccctg
     5581 attgttgaga acgcaggctg gggatccacg tcggctgcgc cgattgcacg acgcgtgttt
     5641 gactactggc ttcttggaca gtaccccagc gaagaagatc tcgccgcagt acgcaagggc
     5701 gaagcgaccg cgcccattgg caagcctcgt acagcgtcag aaattccctg gccgcgctga
     5761 gcagatagtt ttgcacctca acgcaggaat gcgtcgtagc cggttttcag aatcagaact
     5821 gagaccacag cgatgaagac gccgcgtacg aagccggtgc cgaggcgcag tgccaggtgc
     5881 gtaccggcca ggctccccac cacgttggcg acggccatga ccagtgcgaa gtgccaccac
     5941 acatggccct tgagcgtaaa cagtacgagt gcagccaggt tggtggcggt gttgagcagc
     6001 ttggcacctg cggacgcatg cagaaagtcg tagcctagca gccgcaccag caggaacaca
     6061 aaaaagctgc cggtcccggg gccaaagaag ccatcgtaaa aaccgatcac cgcgccaatg
     6121 ccgatggcga tcagcgtttc ctgcaggcct gaaaagcgcg gtgcatggtg ccgccccagt
     6181 tcctttttta gcagggtgta gacaagtacc agcaacagta cgcccggcag caccttgcgc
     6241 agaaactccg gcgagatgac agtcaccagc caggcgcccg ccagcgaggc tgcaaagccc
     6301 gctacggcgg ctggccccaa agcatgccag ggcatggtga cacgccggct gtactgcacc
     6361 gtggcgatgc cggttcccca gaccgaggcg cccttgttgg tgccaaacag ggtggctggg
     6421 tgggtttggg ggaagacggc aaacagcgcg ggtaccagaa tcaggccgcc gccgcccacg
     6481 atcgagtcca caaaccctgc cagcagcgaa gccagcgaga cgacaagcaa ttccatgcag
     6541 ggcattgtcg cacccataaa aaaagcactg gggttaccag tgctttcgga acctgcatct
     6601 caaggtgcag tgttatatct tgttgttcgc cgctatcgcg tgttctcctc ggcgccttct
     6661 ctcacccggc ttgcgccgtc agttgcctcg cagttgcttt ggctgaagtg cttgcaatgt
     6721 aacccggatg acactccgcg ggtatccgga tttacccttt acagcgcagc actggtgatg
     6781 acggttgggt gcttgcccaa tgcaccgtct tcggcaatcc attgcgcggc tttctcggca
     6841 atcatcaagg tcggcgaatt ggtattgccg ctggtaatga gcggcatcac acccgcatcc
     6901 accacgcgca ggcccgctac cccacgcacc ttcaggcgcg catcgacgac tgccatcggg
     6961 tcatcgtcac ggcccatttt ggtggtgccc acgggatgga agatggtggt agcaatatca
     7021 ccggccagac gcgccagatc tgcatcggac tggaactgcg agccgggctt gtattcctcg
     7081 ggctcgtatt tctgcagggc cggctgggcc acgatgctgc gggtcagccg cagcgaatcg
     7141 gccgcgatct tgcgatcggc gtcggtgctg aggtagttcg gtgcaatggc cggtgcgtcc
     7201 ttgaaatccg cactcttgat ctgcaccgag ccccggctgg tggggttgag gttgcacacg
     7261 ctggcagtga aggcgttgaa gttgtgcagg ggctggccaa aagcatccag actcagcggt
     7321 tgcacgtggt attcaaggtt ggggtggggc tggctggcgt cgctgcgcgt gaaggcaccg
     7381 agctggctgg gtgccatgct catggggcca gaacgtttga gggcgtactc cagtccgatc
     7441 tttgccttgc ccagcaggga gttggcctgg gtgttgagcg tgggcacatg cttgaccttg
     7501 aatactgcac ggatctgcag gtggtcctgc aggttctcgc cgacaccggg caggtccttc
     7561 accacggaca caccgcgggc ttgcagcagg gccgccgggc ccaggccgga gagttgcagc
     7621 agttgcggtg aacccacggc acccgcacac agaatgacct cggcccgtgc cgttgcagtc
     7681 accatctcct ggccgtccca cacctgcacg ccggtgcaac gcagcggctg ggcgtcatcg
     7741 ccctgtgagg gttcgaccac cagcctggac gcctgtgcgc tggtccagag ttcgaagttg
     7801 gggcgtccat agcatttggg gcgcaggaag gctttggccg tgttccagcg ccagccctcc
     7861 ttctggttga cctcgaagta gcccacgcct tcgttgttgc cccggttgaa atcgtcggtg
     7921 gcgggaatgc cggcctgggt tgcagcctgt gcaaacgcat cgagcacatc ccagcgcagc
     7981 cgctgtttct ccacgcgcca ttcgccgccg gcattgtggt ggcgcaggat ggcacggtag
     8041 ggggtactgg cgtcctgcat caatgcgggc gctgtgccgc gggcgccgtg catcgcgctg
     8101 gcgcccttgt agtggtcttc gtggagcttg aagtagggca ggttgttgtc ccaggtccag
     8161 tttgcatccc ccaccagttg agcccactgg tcgtagtcgc gggcctggcc gcgcatgtag
     8221 atcatgccgt tgatgctgct ggagccgccc agcgttttgc cgcgcgggta tttcaggctg
     8281 cgcccattga ggcccggatc ggcttcggtg ttgtacagcc agtcggtgcg cgggttgcca
     8341 atgcagtgca ggtagcctac cgggatgtga atccagtggt agtcgtcctt gcgtccggcc
     8401 tcaatcagca ggacacgttt cgtggcgtcc gaagagagcc ggttggccag caggcagcct
     8461 gcagtgcctg cgccgacgat gatgtagtca aaggtagtgt cgttcatgaa agcctatttt
     8521 ccggcccaga cgggcttgcg tttttcctga aaggctttct ggccttccct ggcatcgtcg
     8581 gtgagtgtga agagggcgat ctggctctcg gtgaacgcca tggactcctc gaaggacatg
     8641 gcctcgacct tcttcatggt gtacaggcca cggcgaatgg ccgcgggcga cttgtcgagc
     8701 aggcggtcca gcagccattg cagcttggcg tccacgtcat cgtccacatg gttgaccagc
     8761 ccgtactgca gcgcctgcgc gctggtaatg ggctcgccgg tgatgcacat ctccaccagg
     8821 acgcgccggg gaatgaggtg ctgcagcacg ctcaggacct gtgccgggaa gacaccgacc
     8881 ttcacttcgg gcagaccgaa taccgcgtgg ccggaggcta ccgccatgtc gcacatgctc
     8941 atgagtccca tgccaccggc catgcaggtg ccgttgacgc gcgcgatcag tgggatgtag
     9001 ctggccttgg ctacgcgcag cagttgcgcc aggtgcccat gtggttcgga atagtcggtg
     9061 gtgaacgcgt tggcggactg cagatcggca cccgcacaaa aggccttgct gcccgcgccg
     9121 gtgaccacga tggcgcggat gtcgcgctgg ctttgtgccg cggcgatgga cttcgccatg
     9181 gccgcgagca cgccgtggct catggcattg cgacgctctt cgcgggtaat ggtcagccac
     9241 agcacggggc cgcggacttc gactgcaagt tccggggtgg attggaatgg ggaatctgat
     9301 gggttcatgt tgtctcctgg ggcgctattt tgcttcacgg tgcttgctgc cgcgttacca
     9361 tccgctgcat taactttagg aagcgctgtc cgccgtggag gatacccagt cccgcatcgc
     9421 ctttgaaacc ctccccgagg gcgaatgtgc cgtggcgctt ggcatctgga cggctgccca
     9481 tctggcggtg ccagaacact gggctgcgtt gcaatcggca ctggcgcagg cggcaacccg
     9541 tgggccggcc ataggttggg atttgcgccg cctgcaacgc ctggaccaca gcggtgccca
     9601 ggtcctgtgg aatgcctggg gccgccactg gccgcagcgg ctggaattgc tcgatgtgca
     9661 gcgggccatg ctggagcgca tcgcccgctt ctcggtgccg ccaccgcggc ccgagcgcag
     9721 ttcgccgctg gatgccatgg agacgctggg ggactgggtg ttcagtatgg gggggcatgt
     9781 ggccggtctc gtgcgcctgc tggggcaact ggtcatcgac ctgctcaaac tggtacgcca
     9841 tccggtcaaa gggccgtggc gcgatgtctc cgggcacctg taccacattg gcgctacggc
     9901 gctgcccatc actgcgctgg tgggctttct gatcggcgtc gtgctggcct acctgatgtc
     9961 gcagcagctg cgccagttcg gggcggatgc atttatcgtc aacatactcg gcatctcgct
    10021 gatccgcgaa ttggggccgg tgctggcggc gatcctgatt gcggggcggt ccggatcggc
    10081 catcacggcg cagattggtg tgatgcgcgt caccgaggag ctcgatgcca tgcgcgtcat
    10141 gggcatcccc aagggctacc ggctggtgat gccgcgtgtg attgccatgg ccgtggccat
    10201 gccgctgatc gcagtgtgga ccaccattgc cgcgctgttt ggcggcatgc tggcagcgga
    10261 cctggccatc ggtctgtccc ccgcctattt cgtgacagcg ctgcccaagg cggtggagct
    10321 gtcgaatctg tggctggcgg tttccaagtc ggtcgtgttc ggtgttctga ttgccctgat
    10381 tggctgccac tacggactgc gtgtgcaacc caataccgag agtctgggcc agggaaccac
    10441 agcgtcggtg gtcacctcca tcaccatcgt gattctggtc gatgctctgt ttgcggttgt
    10501 cttcaagagc gtgggaatat gagcgccgtt cagatcgaaa aactctggtc caccttccgc
    10561 accaacggtg ttgaggcggt gatccaaaag gacctggacc tcaccattgc agaggggg
//
LOCUS       DAOSJM010000031        10592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6138_length_10592_cov_4.657208, whole genome shotgun sequence.
ACCESSION   DAOSJM010000031 DAOSJM010000000
VERSION     DAOSJM010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6138_length_10592_cov_4.657208"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..117)
                     /locus_tag="PKX82_02045"
     CDS             complement(<1..117)
                     /locus_tag="PKX82_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOF51056.1"
                     /translation="MSYTAETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQI"
     gene            complement(300..1067)
                     /locus_tag="PKX82_02050"
     CDS             complement(300..1067)
                     /locus_tag="PKX82_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF51057.1"
                     /translation="MDIRNKVAIVTGGASGIGRSIARRFAADGARGVVVADVNLEHAQ
                     AVADEIKGIAVRCDVSKEADIQALVATTRQHYGQVDAYISNAGILGRMGGLDLEDTLW
                     DAMWNIHGMAHVWAARAVVPEMVARGEGFFMVTASAAGLLTIVESAPYAVTKHAAVAF
                     AEWLRIAYGRKGVQVACLCPQSVQTNMISGTDGGSAAVNGILAPEVVAQEVVSTMDNG
                     KFLVLPHPEVAQYFRNKGQDYERWIGGMQKMYARHNG"
     regulatory      1155..1242
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            1318..2769
                     /gene="ahcY"
                     /locus_tag="PKX82_02055"
     CDS             1318..2769
                     /gene="ahcY"
                     /locus_tag="PKX82_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOF51058.1"
                     /translation="MSALKPASTTEDYKIADLSLAQWGRKELAIAETEMPGLMAIREE
                     FAKAQPLKGARITGSLHMTIQTGVLVETLQALGADVRWASCNIFSTQDHAAAALVAKG
                     TPVFAYKGETLDDYWDYTHRIFEFGGKKGSAAEGPNMILDDGGDATLLMHLGARAEKD
                     IKVLAKPGSEEEICLFKAIKAKLKVDPTWYSRRLKNIIGVTEETTTGVMRLEEMSAKG
                     TLAFRAINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMIAGKVAVVAGYGDVGKGSA
                     QALRALSAQVWVTEIDPINALQAAMEGYKVVTMEYAADKADIFVTATGNKNVITYKHM
                     EAMKDQAIVCNIGHFDNEIDVASLEKLKWEEIKPQVDHVIFPAKGKKPSKRIILLAKG
                     RLVNLGCGTGHPSFVMSSSFANQTIAQIELFTKPKEYKNGKVYVLPKHLDEKVARLHL
                     NKVGAMLSELSDEQAAYLGVSKSGPYKKDTYRY"
     gene            2782..3681
                     /locus_tag="PKX82_02060"
     CDS             2782..3681
                     /locus_tag="PKX82_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOF51059.1"
                     /translation="MRIDQLLVQRGLASTRSQAQRLIADGVEWLNLDTWKRVAKNGDE
                     VPESADIRLLDDSEARYVSRGGLKLEAALKTVGLSVAGLRCLDVGQSTGGFTDCLLKS
                     GALSVVGVDVGSAQLHPSLRQDPRVLCVEGVNARTLSATDLIAAYAKSTGAEGHFDVE
                     DASSDDWDEDLDDEDAAEADDEPEAVELPAEFAPPFDLLVADLSFISQTLVLPAAVPL
                     LKDGGTLLTLVKPQFELQPGQVGKGGIVKDPEMYPIVEQRLRDTCADLGLKVTHWFDS
                     PIAGGDGNREFFICARKTGTKKV"
     gene            3695..4540
                     /gene="metF"
                     /locus_tag="PKX82_02065"
     CDS             3695..4540
                     /gene="metF"
                     /locus_tag="PKX82_02065"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOF51060.1"
                     /translation="MQNNDHFPVSLEFFPPKTPEGAEKLRIARQQLYALQPEFCSVTF
                     GAGGSTQEGTFNTVRDILAEGVSAASHFSCVGATKETVRAQLATLKAMGVKRLVALRG
                     DLPSGYGAGGEFQYASDLVAFIRAETGNDFHIEVAAYPEVHPQAKSPASDLQAFATKV
                     KAGADSAITQYFYNADAYFRFQDEVDALGITVPVVPGIMPITSSTQLMRFSDTCGAEI
                     PRWIRLRLQSFGDDTASIKAFGLDVVTDLCEQLRAGGAPAIHFYTMNQSAPTLALCDR
                     LGLLS"
     gene            4630..6054
                     /locus_tag="PKX82_02070"
     CDS             4630..6054
                     /locus_tag="PKX82_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOF51061.1"
                     /translation="MRLAFGMRLGWLCLGAVWGGLALAQPAGSAPLPMGFETAWEQLR
                     AHSGKLAAAQSALQGKQLQAEGLQGLGGPSVAVSAATYSYNANLTLDLDPINQRLAQL
                     GQQFQAPMAGVPVTPVTVGSLPQSYTYNHHGSGNTASVSLMWPLYVGGATQAARALVA
                     AQGDEAQADAQRTGHELMTLLVQRYFGAQLAARAASLRQAAYGNVQEHDRAVEKMLAA
                     GVVSRVERLQARAAVEDARRNALKAQDDAELSSIALARTVGASAQVIPQSPLFVWSTP
                     VQPMDYFLQAALQHHPGLAKVAAKKAQAESLHAGEEGLRKPQVFAFGQHALTSGNPDW
                     VAGVGARWTLYDPVDRDKMAAASQQQIAQAEHTDAQARNDIALLVERNWRVLEQARRQ
                     FLSMQPGAEVADEVLRLRKAGLREGTSTAVDLMDAEMYVVKVQTERAQAAYDYVVALA
                     SLLEGCGLSEEFAAYMARADVKVE"
     gene            6057..7040
                     /locus_tag="PKX82_02075"
     CDS             6057..7040
                     /locus_tag="PKX82_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOF51062.1"
                     /translation="MQHKTLAVGALAVAVVGFLGYGLWRAGQPAPEVFQGQMEARETD
                     IAAKVPGRIAQLLVKEGDLVQVGTPLIRMDSPEVNAKLAQATAAQEAAQAVAAKAQAG
                     ARPQEVEMARMNWQRAEAAAELAKVSFQRVENLSRDGLVPAQKRDEAEANFKASRDAA
                     IAAKAQYDMARTGARAEDKAAASAQVRQVSGVVAEVEAAKAETELHSPIAGEVSKVLA
                     KAGELSPQGVALVSVVDLKDQWVVLNVREDRLQRFALGSEFDATLPALRDNGVLRTVR
                     FKVFYSAPLPDFATWRATRSGQGFDARTFEVRARPLAPVADARPGMSVLVQ"
     gene            7070..8281
                     /locus_tag="PKX82_02080"
     CDS             7070..8281
                     /locus_tag="PKX82_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51063.1"
                     /translation="MPLAPVDSAQGAINVVAFSARREVALLRSRPWDLAMITWVPALA
                     CLLLWWIFSAGLPTRLPIGLVDDDHSALSRQLTRFLDATPGLAVAVRYANAAEAERGL
                     RSAEVYAVVRIPGDFTRTVKQGRAAQVTLLHNAQLGTHSGLIQRDVRTVVGTLSAGIE
                     MAARNKRGEPAQTVRVSMEPVRTGMVTLFNASLDYEQFLATALLPALLHILAMTAGAW
                     SVGRELRDRTVAQWLGAHPTFRQVLGAVAGKLWWSWLGLGLVGTAALVGITWGRGWHP
                     AGSVAWTCLALWVFLALSIAMGALAASATRSLRTALSATGFITAPAFAFGGVGFPILA
                     MPWGAQLWARALPFTHYIRVQMEQLQMGAPLAVSLATPLWMLLASALLLLASTLGLRA
                     AADRPATWGKR"
     gene            8282..9421
                     /locus_tag="PKX82_02085"
     CDS             8282..9421
                     /locus_tag="PKX82_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51064.1"
                     /translation="MATFARSWWNTLQAVWADKGVLLLLVIAPVLYGFFYPWPYMQQA
                     VTRVPVAVVDLDHSSLSRQITRFASANPRLDVRWVGGDLHAAQQALWAGEIEGFAVLP
                     QDLKRHVVRGQSAVVTVEGDGAYALLNKAVLYGFAEAVGTVSAGVEIKKLQAAGQGAR
                     QAAASRNPVGTQMLPLFNPTEGYGSYVVPAVALLILQQTLLMGAAMLVGTWVEVGQHR
                     ASPAQWLGRIAALASVGAMAGLFYFGWEFTVQDFPRGGNPLGALVLLAVYMPAVACLG
                     CLLGVWFADRERSMQVLLLTSLPMAFVSGFSWPSEALPWPLQGLRWLIPSTSGIQASL
                     RLNQMGAPLGDVAGPLTVLLALALGLGALLLWWARPRGAGVRPAL"
     gene            complement(9425..10318)
                     /gene="rlmJ"
                     /locus_tag="PKX82_02090"
     CDS             complement(9425..10318)
                     /gene="rlmJ"
                     /locus_tag="PKX82_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HOF51065.1"
                     /translation="MFSYRHAFHAGNHADVLKHTVLLAVLRHMTQKEAALNVFDTHAG
                     AGLYRLDGDYAKTSAEAADGFLKLVATQPKEPYAPALKDYIDMVAGFNTTNHWSVYPG
                     SPFIIQSLLSGRDKLKLWEMHPTDIKTLTSNIAQLEAGRQVAILREDGFEGLKKFLPP
                     PSRRALVLCDPSYEVKHDYARVVAMVADAMQRFATGTYAVWYPIIPRPEAHDLPKKLK
                     TLANKSGKAWLHATLTVKSSKLTQDEQGEVVRPGLPASGMFLINPPHTLKAALKDALP
                     QMVELLGQDSNAAFSLESGGG"
BASE COUNT         1834 a         3194 c         3616 g         1948 t
ORIGIN      
        1 aatctggatg gcacccgaga acagcaacag cttttcggtc agcgtggtct tgccggcgtc
       61 cgggtgggaa atgatggcaa aggtgcggcg gcggcgggtt tcggcggtgt aggacacagg
      121 gggattccag aaggtggggc aagggatgcg tgcgcacccc tgcgggcgtt cgcccggaag
      181 gggccgattt taccggagcc tgccagcctg cagcgaagga ggtttcaccg ccgggccgcc
      241 ccaaggtgaa acgcggcccc tcgggggcag ggagctacac gcagtgagcg accgtggggc
      301 taaccgttat gtcgggcgta catcttttgc atgcccccaa tccagcgctc gtagtcctgc
      361 cctttgttgc ggaagtactg ggccacttcg gggtgaggta acaccaggaa cttgccgttg
      421 tccatggtgg agaccacttc ctgcgccacc acttcgggag cgaggatgcc gttgacggct
      481 gccgagccac cgtcggtgcc ggaaatcatg ttggtctgca ccgactgggg gcacaggcaa
      541 gccacttgca cacccttgcg gccgtaggca atgcgcagcc attcggcaaa ggccaccgcc
      601 gcgtgcttgg tcacggcata aggtgcggac tcaacgatgg tcagcaggcc ggccgccgag
      661 gcggtcacca tgaaaaagcc ctcgccgcgc gccaccatct cgggcaccac cgcgcgggcc
      721 gcccacacgt gggccatgcc gtggatgttc cacatggcat cccacagggt gtcttccaga
      781 tccaggccgc ccatgcgccc cagaatgccg gcgttggaga tataggcgtc cacctgcccg
      841 tagtgctggc gcgtggtggc taccagggcc tggatgtcgg cttccttgct gacgtcgcag
      901 cgcacggcaa tgcccttgat ctcgtcggcc acggcctggg catgctccag gttcacatcg
      961 gccacgacca cgccgcgggc gccatcggct gcaaagcggc gtgcaatgct gcggccaatg
     1021 ccgctggcgc caccggtgac gatggcaact ttgtttcgga tatccatgaa ttgctcctgt
     1081 tcgggccgca agtgtcggat gtgtggctat tgcggtctgc cgcacaagtg acacgcgact
     1141 ccgtataatc catggtttcc gaggagcgtt gcagttcaca ccgtgtgaat gaggctcgga
     1201 ccgagtgggg ttctcccacc gcatcaacgg cgctcaccca ctggtctgtg cggtgagttt
     1261 tcatctggat ttccccgcag ggccggtggc ttttcaaatt tatttttgga gtaagccatg
     1321 agcgcattga aacctgcaag caccaccgaa gactataaaa ttgccgacct gagcctggcc
     1381 caatggggcc gcaaggaact ggcgattgcc gagaccgaaa tgcctggcct gatggccatc
     1441 cgtgaagagt tcgccaaggc ccagcctcta aagggcgcgc gcatcaccgg ctctctgcac
     1501 atgaccatcc agactggcgt gctggtcgag accctgcaag ccctgggcgc agatgtgcgt
     1561 tgggcatcgt gcaacatctt ctctacccaa gaccacgcgg ccgcagcgtt ggtggccaag
     1621 ggcacccccg tgtttgccta caagggtgaa accctggacg actactggga ctacacccac
     1681 cgcatctttg aatttggcgg caagaagggc agcgcggccg aaggccccaa catgatcctg
     1741 gacgacggcg gtgatgccac gctgctgatg cacctgggcg cccgcgccga aaaggacatc
     1801 aaggtcctgg ccaagcccgg cagcgaagaa gaaatctgcc tgttcaaggc catcaaggcc
     1861 aaactcaagg tcgatcccac ctggtacagc cgccgcctca aaaacatcat tggcgtgacg
     1921 gaagagacca ccaccggcgt gatgcgcctg gaagaaatgt ccgccaaggg cacattggct
     1981 ttccgcgcca tcaacgtgaa cgactcggtg accaagagca agttcgacaa cctgtatggc
     2041 tgccgcgaat cgctggtcga cggcatcaag cgcgccaccg acgtgatgat cgccggcaaa
     2101 gtggccgtgg tcgctggtta cggtgacgtg ggcaagggct cggcccaggc actgcgcgcc
     2161 ctgagcgccc aggtgtgggt gaccgagatt gatcccatca acgccctgca ggctgctatg
     2221 gaaggctaca aggtcgtcac catggaatac gccgctgaca aggccgacat tttcgtgacc
     2281 gccaccggca acaagaacgt catcacctac aagcacatgg aggccatgaa agaccaggcc
     2341 atcgtttgca acattgggca cttcgacaac gaaatcgacg ttgcctccct ggaaaagctg
     2401 aagtgggaag aaatcaagcc ccaggtggac cacgtgatct tcccggccaa gggcaagaag
     2461 ccttccaagc gcatcatcct gctggccaag ggccgtctgg tgaacctggg ttgcggcacc
     2521 ggccacccca gctttgtgat gtcttcgagc tttgcgaacc agacgatcgc ccagatcgaa
     2581 ctgttcacca agcccaagga gtacaagaac ggcaaggtct atgtgctgcc caagcacctg
     2641 gacgagaaag tggcgcgcct gcacctgaac aaggttggcg ccatgctgtc cgagctgtct
     2701 gatgagcagg ctgcctacct tggcgtcagc aagtccggcc cgtacaaaaa agacacttac
     2761 cgctactaag ccaaggcctg catgcgtatt gaccaactgc tggtccagcg tgggctggcc
     2821 agtacccgct ctcaggccca gcgcctgatt gcggatggcg tggagtggct gaacctggac
     2881 acctggaaac gcgtggccaa gaacggcgac gaggtgcccg aatcggccga catccgtctg
     2941 ctggacgact ccgaggcgcg ctatgtgtcc cgcggtggtc tgaagctgga ggcagcactc
     3001 aaaaccgtgg gcctgtcggt ggcagggctg cgctgtctgg acgtggggca gagcaccggt
     3061 ggcttcaccg actgcctgct caagtctggc gcgctgtccg tggtcggtgt ggatgtgggc
     3121 agcgcccagt tgcaccccag tctgcgtcag gacccgcgcg tgctgtgcgt cgaaggcgtc
     3181 aatgcccgta cgctaagtgc tactgatttg atagctgctt acgcaaagag cacgggggct
     3241 gaaggccatt ttgatgtgga agatgcgtct tccgacgact gggacgaaga cctggacgat
     3301 gaggatgctg ccgaggcgga tgacgagccc gaagcggtgg agttgcctgc ggagtttgcc
     3361 cccccgttcg acctgctggt agcggacctg tccttcatct cgcagacgct ggtgctgccg
     3421 gcagccgttc ccctgttgaa agacggtgga acgctgctga cgctggtcaa gccgcagttc
     3481 gagttgcagc ccggccaggt gggcaagggc ggcatcgtga aagaccccga gatgtacccc
     3541 atcgtggaac agcgcttgcg cgacacctgt gccgacctgg gcctgaaggt gacccactgg
     3601 ttcgactcgc cgatcgctgg tggcgatggc aaccgtgaat ttttcatctg tgcccgcaag
     3661 accgggacca agaaagtctg agaggggttt ccccatgcag aacaacgacc acttccccgt
     3721 cagtctggaa ttcttcccac ccaaaacgcc cgaaggtgcc gagaagctgc gtattgcgcg
     3781 ccagcagctc tacgcactcc agcccgagtt ctgttcggtg acctttggtg ctggtggttc
     3841 aacccaggaa ggcacgttca ataccgtgcg tgacattctg gccgagggcg ttagcgcggc
     3901 ttcgcacttc tcctgtgtcg gcgcgaccaa agagacggtg cgtgcccagc tggccaccct
     3961 caaggccatg ggcgtaaagc gtcttgtggc cttgcggggt gatttgccca gcggctacgg
     4021 tgccggcggc gagttccagt acgccagcga tctggtggcg tttatccgtg ccgagacggg
     4081 caatgacttc cacattgaag tggcggccta ccccgaagtg catccgcaag ccaagtcacc
     4141 cgccagcgac ctgcaggcgt ttgccaccaa ggtcaaggca ggtgcggatt cggccatcac
     4201 ccagtacttt tacaacgcgg atgcgtactt ccgcttccag gacgaggtgg atgcgctggg
     4261 tatcacggtg ccggtcgtgc ccggcatcat gcccatcacc agctcgacgc aactcatgcg
     4321 tttttccgac acctgtgggg ccgagattcc gcgctggatc cgtctgcgtc tgcagagctt
     4381 tggtgacgat acggcgtcca tcaaggcgtt tggcctggat gtggtgacgg acttgtgcga
     4441 gcaactacgt gcgggtgggg cgcctgccat ccacttctac accatgaacc agagcgcgcc
     4501 gacgctggcg ttgtgcgatc ggctggggct gctgtcctga tgcacccaac ccgctgaccc
     4561 cataatggaa gcgattccgc cttggggcgg ggggctgcga gaggttgcga tggtcacgat
     4621 aggaaaacca tgagattggc gtttggcatg cgtctgggat ggttgtgcct cggtgccgtg
     4681 tggggtggtc tggcgctggc tcaacccgca ggatccgcgc cgctgcccat ggggttcgaa
     4741 accgcctggg agcagcttcg cgcgcattca ggcaagctgg cggcagccca gtcggcactg
     4801 cagggcaagc agttgcaggc cgagggcctg cagggactgg gaggcccctc cgtggcagtg
     4861 tcggccgcca catattccta caacgccaac ctcacactcg acctggaccc gatcaaccag
     4921 cgcctggccc agctcggcca gcagtttcag gcgcccatgg caggagtgcc tgtgacgccg
     4981 gtcactgtcg gctccctgcc gcaaagctat acctacaacc accatggcag cggcaacacg
     5041 gcttcggtat cactgatgtg gccgctgtac gtgggcggcg ccacccaggc cgcgcgcgcg
     5101 ctggtggcgg cacagggaga cgaggcccag gccgatgccc agcgcaccgg gcatgaactg
     5161 atgacgctgc tggtgcagcg ctatttcggc gcccaactgg ccgcgcgcgc agcgagcctg
     5221 cgccaggccg cttacggcaa tgtgcaggag cacgatcgtg cggtggagaa gatgctcgcg
     5281 gctggcgtgg tgtcgcgcgt ggagcggctg caggcccgcg ccgcagtcga agacgcacgg
     5341 cgcaatgcgc tcaaggccca ggacgatgcg gagctctcca gcattgcgct ggcacgtacc
     5401 gtgggcgcca gcgcgcaggt gattccgcag tcgccgctgt ttgtctggag caccccggtg
     5461 cagccgatgg actacttcct gcaggcggcc ctgcagcacc atccggggct ggccaaggtg
     5521 gcagccaaga aagcgcaggc cgagagcctg catgccggcg aagaaggtct gcgcaagccc
     5581 caggtattcg ctttcggcca gcacgcactg acctccggta acccggactg ggtggcgggc
     5641 gtgggcgcac gctggacgct ctacgacccg gtggaccgcg acaagatggc tgccgcctcg
     5701 cagcagcaga ttgcccaggc cgagcacacc gatgcccagg cccgcaacga cattgccctg
     5761 ctggtggaac gcaactggcg tgtgctggag caggcgcggc gccagttcct gagcatgcag
     5821 cctggtgccg aagtggccga cgaagtgctg cgcctgcgca aggccggact gagggagggc
     5881 accagcacgg cggtggacct gatggatgcg gaaatgtatg tggtcaaggt gcagaccgag
     5941 cgcgcccagg cggcctacga ctacgtggtg gcactggcca gcctgcttga aggctgtggt
     6001 ttgtctgaag agtttgccgc ctacatggcg cgcgccgatg tgaaggtgga atgacgatgc
     6061 agcacaagac gttggccgtg ggcgcactgg ccgtggcagt ggtgggattt ctgggctacg
     6121 gtctgtggcg tgccgggcaa ccggcccccg aggtgttcca ggggcagatg gaggcgcgcg
     6181 agaccgacat cgccgccaag gtgcccggcc gcatcgcgca actgctggtc aaggaaggtg
     6241 acttggtgca ggtcggcaca ccgctgatac gcatggacag cccggaggtc aacgccaagc
     6301 tggcccaggc cacggccgcg caggaggccg cccaggccgt ggcggccaag gcccaggcgg
     6361 gcgcccgtcc gcaggaagtg gagatggcac gcatgaactg gcagcgcgcc gaggccgccg
     6421 ccgagctggc caaggtgtca ttccagcgcg tcgagaacct gtccagggat gggctggtgc
     6481 cggcccagaa gcgtgacgaa gccgaggcca atttcaaggc ctcgcgtgac gccgccattg
     6541 ccgccaaagc ccagtacgac atggcccgca ccggtgcccg tgccgaggac aaggccgctg
     6601 ccagcgccca ggtgcgccag gtcagcggtg tggtggctga agtggaggcc gccaaggccg
     6661 agaccgaatt gcacagcccg atcgccggcg aggtgtccaa ggtattggcc aaggcgggcg
     6721 aactgtcccc gcagggcgtg gccctggtca gcgtggtgga tctgaaagac cagtgggtgg
     6781 tgctcaatgt gcgcgaagac cgcctgcagc gttttgcgct gggctccgag ttcgatgcca
     6841 cgctgccggc gctgcgcgac aacggtgtgt tgcgcacggt gcgcttcaag gtgttctaca
     6901 gcgcgccact gcctgacttt gccacctggc gcgctacgcg cagcgggcag ggctttgatg
     6961 cccggacctt cgaagtgcgg gcccgtccgc tggcccctgt cgcggacgcc cgccctggca
     7021 tgagtgtgct ggtgcagtag gctcatcgac tgcggatggg tagaaaaata tgccactagc
     7081 ccccgtggat agtgcgcaag gcgctataaa tgttgtagcg ttttctgcgc ggcgcgaagt
     7141 ggcgctgctg cgctcgcgcc cctgggatct ggccatgatt acctgggtgc cggcgctggc
     7201 ctgcctgctg ctgtggtgga ttttttcggc cgggttgccg acgcgcctgc ccatcgggct
     7261 ggtggatgac gaccattccg ccctgtcgcg ccagctcacc cgatttctgg atgccacgcc
     7321 gggcttggcg gtggcagttc gctatgccaa tgccgccgaa gccgagcgcg gccttcgcag
     7381 tgccgaggtg tacgcggtgg tgcgcattcc cggtgatttc acgcgcacgg tcaagcaggg
     7441 gcgtgcggcc caggtcacgc tgctgcacaa cgcccaactg ggcacgcact cgggactgat
     7501 ccagcgtgat gtgcgcaccg tggtcggtac gctgtcggcg ggcatcgaga tggcggcacg
     7561 caacaaacgc ggcgaacccg cacagacggt gcgcgtgagc atggagccgg tacgtaccgg
     7621 catggtgacg ctgttcaatg catcgctcga ctacgagcag ttccttgcga cagcgctact
     7681 accggccctg ctgcacattc tggccatgac agccggggcc tggagcgtgg ggcgcgaact
     7741 gcgcgaccgc acggtggcgc agtggctggg cgcgcacccc actttccgcc aggtgctggg
     7801 cgccgtggcg ggcaagctgt ggtggtcctg gctgggcttg ggactcgtag gcacggctgc
     7861 gctggtgggc atcacctggg ggaggggctg gcatccggcc ggatcggtgg cgtggacctg
     7921 cctggccctg tgggtgtttc tggcgctgtc gatcgccatg ggtgctttgg cggcttcggc
     7981 cacgcgctcg ctgcgcacgg cgttgtcggc caccggcttc atcacggccc cggcatttgc
     8041 gttcggtggc gtgggctttc cgattctggc catgccctgg ggggcgcagc tgtgggccag
     8101 ggcgctgccg tttacccact acatccgtgt gcagatggag cagttgcaga tgggtgcgcc
     8161 gctggcggtg tcgctggcca cgccgctgtg gatgctgctg gcttcggcgc tgttgctgct
     8221 ggccagtacc ctggggttgc gcgccgccgc tgaccgaccg gccacctggg ggaagcgctg
     8281 aatggcgact ttcgcacgct catggtggaa caccctgcag gcagtctggg ctgacaaggg
     8341 ggtgctgttg ttgctggtca ttgcgcccgt gctgtacgga ttcttctacc cttggccgta
     8401 catgcagcag gcggtaacgc gcgtgccggt cgcggtggtg gatctggacc acagcagcct
     8461 gtcgcgccag atcacgcgtt ttgccagtgc caacccgcgc ctggacgtgc gctgggtggg
     8521 tggtgacctg catgcggcgc aacaggcgct gtgggcgggc gagatcgagg gatttgcggt
     8581 gttgccgcag gacctcaaac ggcatgtggt gcgtggccag tctgcggtgg tcacggtgga
     8641 aggggatggt gcctatgccc tgctcaacaa ggccgtgctg tacggatttg ccgaggcggt
     8701 gggcacggtg tctgccgggg tggagatcaa gaagctgcag gcggccggtc agggtgcgcg
     8761 gcaggccgcg gccagtcgca atccggtggg cacgcagatg ctgccgctgt tcaacccgac
     8821 ggagggctac ggcagctacg tggtgcctgc cgtggccctg ctgatcctgc agcagacgct
     8881 gctgatgggg gcggccatgc tggtggggac ctgggttgag gtggggcagc accgcgccag
     8941 ccctgcgcag tggttggggc gcattgccgc gctggccagt gtgggcgcaa tggcggggct
     9001 gttctatttc ggctgggaat tcaccgtgca ggacttcccg cgtggcggca atccgctggg
     9061 ggcactggtg ctgctggcgg tctacatgcc ggcagtggct tgtctgggat gcctgttggg
     9121 ggtgtggttt gccgatcggg agcggtcgat gcaggtgctg ttgctcacct cgctgccgat
     9181 ggcgtttgtg tccggctttt cctggccgtc cgaggccctg ccgtggccgc tacaggggtt
     9241 gcgctggctg atccccagta cctcgggcat ccaggcgtcg ctgcggctca accagatggg
     9301 cgcaccactg ggcgatgtgg cgggtccgtt gaccgtgctg ctggcgttgg cgttgggctt
     9361 gggtgcgctg ctgctgtggt gggcccggcc acgcggtgcg ggcgtccggc cggcgctgtg
     9421 aaggtcagcc gccgccagat tccaggctga aggccgcgtt gctgtcctgg cccagcagtt
     9481 ccaccatttg cggcaaagcg tctttgaggg cggccttgag cgtatgcggt gggttgatca
     9541 ggaacatgcc gctggcgggc agaccggggc gaaccacttc gccttgttca tcctgcgtca
     9601 gcttgctgga cttgacggtc agcgtggcat gcagccaggc tttgccggac ttgttggcca
     9661 gggttttgag cttcttgggc aggtcgtgcg cttcgggacg cgggatgatg gggtaccaca
     9721 ccgcataggt gccggtggca aagcgctgca tggcgtcggc aaccatggcc accacgcggg
     9781 cgtagtcgtg tttgacctcg tagctggggt cgcacagcac cagtgcgcgg cgcgacggcg
     9841 gtggcaaaaa ctttttcagg ccttcaaagc cgtcttcacg caggatggcg acctggcgtc
     9901 cggcttcgag ctgggcgatg ttggaggtca gtgtcttgat gtcggtggga tgcatttccc
     9961 acagcttgag cttgtcgcgg ccgctcagca ggctctggat aatgaagggc gagccggggt
    10021 acacgctcca gtggttggtg gtgttgaagc ccgccaccat gtcgatgtaa tcctttaggg
    10081 ccggggcgta aggctctttg ggctgggtgg ccaccaattt gaggaagccg tccgccgcct
    10141 cggcgctggt cttggcgtaa tcgccgtcca ggcggtacag gccggcaccg gcgtgggtgt
    10201 caaacacatt gagtgcggcc tctttttgcg tcatgtggcg cagcaccgcc agcaaaacgg
    10261 tgtgcttgag aacgtcggca tggttgccgg catggaaggc gtggcgataa ctgaacatgg
    10321 gggaatggta acctttcagg ccccaaattg cctaaattgc caaggtttcg tataatcaaa
    10381 ggcttcgcag cgattttgtg gctccgcggc gaagcaactc tcagtatcgt ctcacgaccg
    10441 tgcgccgagt gatccccacc taagagatcc cattagagga acgccgaccg tggaaaagag
    10501 cccgacaaac cttcttttaa agagaaatct catgtctact ttcagcgcaa aacccgctga
    10561 ggtcgtgcac gagtggtttg tgattgacgc ga
//
LOCUS       DAOSJM010000032        10481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6211_length_10481_cov_4.444658, whole genome shotgun sequence.
ACCESSION   DAOSJM010000032 DAOSJM010000000
VERSION     DAOSJM010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10481
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6211_length_10481_cov_4.444658"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PKX82_02095"
     CDS             <1..619
                     /locus_tag="PKX82_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOF51066.1"
                     /translation="NGWGILGVTAPLLVLAPRLMWAWLCAAPFAILFARTGKFFLESP
                     RPAAVVDNAQMRIVGEVLHNVSMPSGHTLTAFAVASGIFFAIPRARRMRHIWLLVLAA
                     GTGLSRIAVGAHWPGDVAVGASLGLLAGLLGNQLLARMAPVHCQPTHWSLRLVAVLVA
                     LAVYHLLSEELDFAENGPLQLLLAPVAVVSLVVFAIRNARALKAA"
     gene            complement(538..1395)
                     /locus_tag="PKX82_02100"
     CDS             complement(538..1395)
                     /locus_tag="PKX82_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOF51067.1"
                     /translation="MQTLNLIGAGRVGQTLAHLWHARQVFAIQHLHTRHLQSAQTACD
                     FIGAGHPIVTLDAMATADVWMLAVPDAGIAQAAADLAAHAQAHHLPPAIALHCSGALT
                     ADVLAPLKNLGWQVASAHCILSFATPALAVQQFPGTACALEGDADACALLRDAFTRIG
                     AQCFDVASTDKVLYHAAAVFATNFLPVLESLAESAWQTTGVPASLIPSLRASLMSNAV
                     ANIQRLGPAAALTGPAARGDLTAIARQTQVVTAWDPQAGAAYAALSALALRMANTTKD
                     TTATGANSS"
     gene            complement(1398..2447)
                     /locus_tag="PKX82_02105"
     CDS             complement(1398..2447)
                     /locus_tag="PKX82_02105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOF51068.1"
                     /translation="MLHAFESLTPDLVLDALDSVGLRGDGRLTALSSYENRVYQVHLE
                     DGTQVVTKFYRPGRWSDAQILEEHAFAAELMAAEIPVIGPLQLTSQLAAEGPHTLHHF
                     GDFGFSVSPRRGGRPPELDDGEVLEWIGRFLARIHSVGAQRPFAHRPTLNLESFGIEP
                     MQWLLEHDMVPLDVQSTWTRACQNAIDLIATYAPLTGAEGQKHSNSSGIRLLRLHGDC
                     HPGNILWTPLDAAGDAGPGPHFVDLDDARMGPAVQDLWMLLSGDRQQRTRQLGALVDG
                     YEQFREFDRAELALIEPLRTLRLIHYSAWLARRWSDPTFPANFPWFGSSDYWHGQVLM
                     LEEQMEAMQEDPLVV"
     gene            complement(2478..3995)
                     /locus_tag="PKX82_02110"
     CDS             complement(2478..3995)
                     /locus_tag="PKX82_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/universal stress protein"
                     /protein_id="HOF51069.1"
                     /translation="MKLLEPGTLLDGFTIEECIHSGGMAHIYQVRYTDSRFSVGFPMA
                     MKIPRMTAGDGAENIVSFEVECQIMQALTGNHMPRFVAAGDLDRVPYLVMEYVHGRTL
                     QHWLDSELPPSMDEVVRLGASMAQAAHALHQQNAVHLDLKPANVLFRTDGSAVLLDFG
                     LSWHAHYPDLLAEQLRKAVGSPAWIAPEQVVGVRGDPRSDIFAIGVMLYQLCTRELPF
                     GEPQTPAGLRQRLWMDPPPPRKVNPDVPTWLQEVVLRCLEPEAVKRYPSAAHLAFDLM
                     HPTQVQITERGTNVTGTKFTTHFKRWIKAAGMHYQPSPVPSQQIDEVPIVMVAVPHKD
                     VTDATLYSLRQAVARSLGTRPGARLACVTVISSGQTSASDEEKSETNVHRRYLTALHQ
                     WAQPLEHPSNQISCHVLESGDPAEALLDYARGNNVSVIVMGAATHGLKTQRFIATVPI
                     KVAMDAPCTVILVKQSLPFEHLGEQTEREKPAASGYSDSDTETPSVYGDDLGPLG"
     gene            complement(3992..4744)
                     /locus_tag="PKX82_02115"
     CDS             complement(3992..4744)
                     /locus_tag="PKX82_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOF51070.1"
                     /translation="MRLALLSDIHGNIQALDACLAHARAQRAQRFAFLGDLVGYGADP
                     AAVVDRVMLMTEEGAIVLKGNHDAMAIHPPAEIKTIGDSTATWTSRQLNKAQRDWLEQ
                     LPLTTQVDNILLVHASADGPELWRYVYDERAATASLDAATANPEIRYVFGGHVHEQTL
                     YYRGATSGLMKFIPQPGVAVPVPRHRQWLSTVGSVGQPRDGKPASMYALLDTDRAQLT
                     FHRVPYNHGHAAAAIRRAGLPEFFAERLEKGR"
     gene            complement(4897..6030)
                     /locus_tag="PKX82_02120"
     CDS             complement(4897..6030)
                     /locus_tag="PKX82_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HOF51071.1"
                     /translation="MHTTDLLVRKDQLTTTRLWTRPAAALADGQVRVQITRFALTSNN
                     ITYAAFGDAMNYWRFFPVLSEDGTPDAAWGRIPVWGFGTVVQSRHADVPVGEHLYGYF
                     PMANAVDLTPTRVTPLSFSDAAPHRAELHAVYNQYTRTRTDPFYRADTEDLQALLRPL
                     FITSWLIDDFLADNDWFGTAATPGRPAVMLLSSASSKTALGTAFQMAQRKNIEVVGLT
                     SPGNVAFCESLGCYHRVLTYDALTSIAEDTPCIYVDFAGNAAFRKTLHTHCKGLAYSS
                     SIGGTHVAELGGGRDLPGPKPTLFFAPAQIKKRSTEWGAAGLGQKLVQSWEGFLAAVT
                     QSTPPWVVVQHHDGAQAVQSAYQTVLAGRGDPRMGHMLSLPVA"
     gene            complement(6122..6550)
                     /locus_tag="PKX82_02125"
     CDS             complement(6122..6550)
                     /locus_tag="PKX82_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HOF51072.1"
                     /translation="MANKIEKVVYRAHAKSTGGRDGTTRTSDGLLDLKLAVPKEMGGA
                     GGGVNPEQLFAAGYSACFIGAMKFVAGTQKIALPADTSIDATVGIGQIPQGFGIEVQL
                     AVNIPGMERAAAEALVQQAHAVCPYSNATRGNIEVTITIV"
     gene            complement(6621..7079)
                     /locus_tag="PKX82_02130"
     CDS             complement(6621..7079)
                     /locus_tag="PKX82_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOF51073.1"
                     /translation="MPSTSTRPLPASALLLDNQLCFALYSASLAMTKLYKPLLEDLGL
                     TYPQYLVMLVLWEQDGLTVSALGERLFLDSGTLTPLLKRMEHSGLVTRQRSAEDERRV
                     EVRLTSDGKKLKTRATSVPACVVEAAGCPISELMTLTHEVQALRNRLTGT"
     gene            complement(7189..7989)
                     /locus_tag="PKX82_02135"
     CDS             complement(7189..7989)
                     /locus_tag="PKX82_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51074.1"
                     /translation="MTNESVWRAPRLSLRFWPVFLRNLLVWRKLAIPSLVGNIAEPLM
                     WLVAFGYGMGALVGQITVDGAQVPYILFLASGSICMSAMQAASFEALYSAFSRMHVQK
                     TWDGIMNAPVGLDDIVMAEMLWAAFKSLFTVTAILGVMLALGISHSPKLLLAWPILLG
                     VGITFSCMALVFNALAKGYDFFTYYFTLFLTPMMFLSGIFFPLDQLPVAVRVVADWLP
                     LSNAVALVRPLFMDQWPAHVLRHALVLVAYATVAFWVALALTRKRFRR"
     gene            complement(7986..8909)
                     /locus_tag="PKX82_02140"
     CDS             complement(7986..8909)
                     /locus_tag="PKX82_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF51075.1"
                     /translation="MSTPLFRVQHLHKRYGDAEVVKDLSFEIEPGECLGVIGPNGAGK
                     TTTIRMCLGLTAPDNGSVEVMGLHMPRDALAIKAQLGVVSQFDTLDPDFTCAENLLVY
                     GRYFGMKDAAIRARIPQLLEFASLTSKASAKPGELSGGMKRRLSLARALVNDPRLLLL
                     DEPTTGLDPQARHLMWERLQLLLQQGKSILLTTHFMDEAERLCTRLLVLDHGRKIAEG
                     KPRDLIARHLEPDVVEVYGNGALALVDSPLRNMAARVEVSGETVFFYTSDAASLLSAL
                     GAHTQLRTLHRPANLEDLFLKLTGRQIREDA"
     gene            complement(8945..10099)
                     /locus_tag="PKX82_02145"
     CDS             complement(8945..10099)
                     /locus_tag="PKX82_02145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF51076.1"
                     /translation="MDQVSVFTVLLLTALNLGTVSVALPLIMGRGVSRAARLAQASLI
                     AQTLGWVAIIASGFWAGHWLDMAISTVSMACAGASNYLMFLALRAWLGRRVGEKLLIA
                     LVILMPLGYALSFSNYSVRVGWANFLLAAQLLLVARATIRHKVDSSRRWRWMLFLCYG
                     AVAGATIGRGILGAFFPESYPHFQSGHPVNMVAQIVANVGQILITVAILVAWREEAEA
                     KLRAQAFTDHLTGLPNRHGWDERARVLFDQAQRHDSPLALIMLDLDHFKRINDTHGHE
                     AGDQVLRMVGRVLQSNRRSSDLAARLGGEEFALLLPQTDMTAAVLFEHRLREAFLRAG
                     QEFPQGAANYSAGLAMLRPSDHSLTELIIRADNGLYQAKALGRGRLELTS"
     gene            complement(10117..>10481)
                     /locus_tag="PKX82_02150"
     CDS             complement(10117..>10481)
                     /locus_tag="PKX82_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOF51077.1"
                     /translation="KSIMRTGTVASTDNRNWELLPNNQPQRRFSQSRAVALDMESATI
                     AANGFRFRVPYGTLLCVSDKPLHGEIKLPGMANHFYRERVDQHLRIGMRAVELLRKQG
                     IDQLHSRKLRSFAEVAFQ"
BASE COUNT         1904 a         3180 c         3470 g         1927 t
ORIGIN      
        1 caatggctgg ggcatcttgg gggtcaccgc gccgctgctg gtgctggcgc cccgcctgat
       61 gtgggcctgg ttgtgtgccg caccgttcgc cattctgttc gcccgcaccg gcaagttctt
      121 tctggaaagc ccacgcccgg cggctgtggt ggacaacgcc cagatgcgca ttgtgggtga
      181 ggtgctgcac aacgtctcca tgccctcggg ccatacgctg acggcctttg cggtggcatc
      241 gggcatcttc tttgccattc cgcgtgcgcg gcgcatgcgc cacatctggc tgctggtgct
      301 ggctgcgggc acggggttgt cgcgcattgc ggtgggcgca cactggcccg gtgatgtggc
      361 ggtgggggcc agcttggggc tcctggcagg tctgctgggg aaccagctgc tcgcgcgcat
      421 ggcgccggta cattgccagc ccacccactg gagcctgcgg ctggtcgctg tgctggtggc
      481 actggcggtg taccacctgc tgtcggagga actcgatttt gccgagaacg ggccgcttca
      541 gctgctgttg gccccggtgg cggtggtgtc cttggtggtg ttcgccatcc gcaacgccag
      601 ggcgcttaaa gccgcatagg ctgcaccggc ctgagggtcc cacgcggtca ctacttgcgt
      661 ctggcgtgcg atggcggtca gatcaccacg cgcagccgga ccggtcagcg cagcggcagg
      721 gcccaaccgc tggatgttgg ccacggcgtt cgacatcagc gaagcccgca aagacggtat
      781 caggctggcg ggcaccccgg tggtctgcca ggcactttct gccagcgact ccagtaccgg
      841 caggaagttg gtggcaaaca cggccgctgc gtggtacagc accttgtcgg tgctggctac
      901 atcaaagcac tgtgcaccga tccgcgtgaa ggcatctcgc aacagggcgc aggcatctgc
      961 gtcgccttcc agcgcacagg ctgtgcctgg aaactgctgc acggccaggg cgggtgtggc
     1021 aaagctcagg atgcagtgcg cactggccac ctgccaaccc agattcttca gcggtgccaa
     1081 cacgtccgca gtcagggcgc cgctgcaatg cagggcgatc gccggtggca ggtggtgagc
     1141 ctgggcgtgg gccgccagat ctgcagcggc ttgcgcaatg cccgcgtccg gtacggcgag
     1201 catccacacg tcggctgtgg ccatggcatc cagggtcacg atggggtgtc ctgcaccaat
     1261 gaaatcgcag gcggtctggg cactctgcag atggcgcgtg tggaggtgct ggatcgcgaa
     1321 cacctggcgc gcgtgccaca ggtgggccag cgtctggccc acacggccag cgccaatcag
     1381 gtttagagtc tgcatgttca tacgaccagc gggtcttcct gcatcgcttc catctgctct
     1441 tcgagcatca gcacctggcc gtgccagtag tcgctgctgc cgaaccacgg aaagttggcc
     1501 ggaaaagtcg ggtcgctcca gcgccgggcc agccaggcgc tgtagtggat caggcgcagg
     1561 gtacgcagcg gttcgatcag ggccagttcg gcgcggtcaa actcgcggaa ctgctcatag
     1621 ccgtcgacca gtgcgcccag ctggcgcgtg cgttgctgcc ggtcaccgct gagcagcatc
     1681 cacaggtcct gcacggccgg gcccatgcgg gcgtcgtcca ggtcgacaaa gtgcggaccc
     1741 gggccggcat cgccggcggc atccagtggg gtccacagga tattgccggg gtggcagtcc
     1801 ccatgcagcc gcagcaggcg gataccagag gaatttgagt gtttttggcc ttcagccccc
     1861 gtcaatggtg cgtaagtagc tattaaatcg atagcgttct ggcaggccct ggtccaggtg
     1921 gactgcacgt ccagcggcac catgtcgtgt tccagcagcc attgcatggg ctcgatgcca
     1981 aagctttcca ggttcagggt cgggcggtgt gcaaagggtc gctgggcccc cacgctgtgg
     2041 atgcgggcca gaaagcggcc gatccattcc aggacttcac cgtcgtccag ttcgggtggg
     2101 cggccaccgc ggcgcgggct gacgctgaaa ccaaagtcgc cgaagtggtg cagagtgtgt
     2161 ggcccttcgg cggcgagttg gcttgtcagc tgcagtgggc cgatcaccgg aatttccgcg
     2221 gccatcagct cggcggcaaa cgcatgctct tccagaatct gggcatcgct ccagcgcccg
     2281 ggtcggtaga acttggtgac aacctgggtg ccatcttcca ggtgcacctg gtacaccctg
     2341 ttttcgtagg acgacagcgc ggtcaggcgg ccatcaccgc gcagacctac gctgtcgagc
     2401 gcatcaagga ccaggtcggg ggtgagtgat tcaaaggcat ggagcatgcc ccgattgtca
     2461 acccaacccg gatccgctta gcccagcggt cccaggtcgt cgccatacac cgatggcgtc
     2521 tcggtgtcgg agtcggaata gccgctggcg gccggcttct cgcgctcggt ctgttcgccc
     2581 agatgttcaa agggcaggga ctgcttcacc aggatgacgg tgcagggtgc gtccatggcg
     2641 accttgatcg gcacggtggc aatgaagcgc tgggtcttga gtccgtgggt cgcggcgccc
     2701 atcacgatca cgctgacgtt gttgccacgg gcatagtcca acaaggcctc ggccgggtcg
     2761 ccggattcca gtacgtggca ggaaatctgg ttgctggggt gttccagggg ctgcgcccat
     2821 tggtgcaggg ccgtgaggta gcgccggtgc acattggtct cgcttttctc ttcgtccgac
     2881 gcactggtct ggcccgaaga aatcacggtc acgcaggcca ggcgcgcgcc ggggcgcgtg
     2941 cccagggagc gggccacagc ctggcgcaac gagtacagcg tggcgtcggt cacatccttg
     3001 tgcggcacgg ccaccatgac gatgggtact tcatcgatct gttgcgaagg caccgggctg
     3061 ggctggtaat gcatgcccgc agccttgatc cagcgtttga agtgggtggt gaacttggtg
     3121 ccggtgacat tggtgccgcg ttccgtgatc tggacctggg tcgggtgcat caggtcaaac
     3181 gccagatggg cagcagacgg gtagcgcttg accgcttctg gttccagaca gcgcagcacc
     3241 acctcctgca gccaggtggg gacgtccggg ttcaccttgc gcggtggcgg tgggtccatc
     3301 cacagccgct ggcgcaggcc tgccggtgtc tggggctcgc caaagggcag ctcgcgcgtg
     3361 cacagctggt agagcatcac cccgatggcg aaaatgtcgc tgcgcgggtc gccccgcact
     3421 cccaccacct gttcgggggc aatccaggcg ggggatccca ccgccttgcg caactgttct
     3481 gccagcaggt cgggatagtg ggcgtgccag gagaggccaa agtccagcag cacggcactg
     3541 ccatcggtgc ggaacagcac gttggctggc ttgaggtcca ggtgcaccgc attttgctgg
     3601 tgcaatgcat gggcggcctg cgccatggat gcgcccagcc ggaccacttc atccatgctg
     3661 ggggggagtt cggagtcgag ccagtgttgc agggtccggc cgtgcacata ttccatcacc
     3721 aggtagggca cgcggtccag atcgcccgca gccacaaagc ggggcatgtg gttgccggtc
     3781 agcgcctgca tgatctggca ctccacttcg aagctgacaa tgttctcggc accgtcgccc
     3841 gccgtcatgc gcgggatttt catggccatg ggaaagccca cgctgaaacg gctgtcggtg
     3901 tagcgcacct ggtagatgtg cgccatgccg ccggagtgga tgcactcctc gatggtgaat
     3961 ccatccagca gggttcctgg ctccagtagt ttcatcgtcc cttctccagt cgttcagcaa
     4021 agaattcggg caagcccgcc ctccggatgg ccgcagctgc atgcccatgg ttgtagggca
     4081 cacggtggaa tgtcagttgc gcccggtctg tgtcgagcag ggcgtacatg ctggcgggtt
     4141 tgccatcgcg gggctggccg accgagccca ccgtgctcag ccactggcga tggcgcggta
     4201 ctggcaccgc cacgccgggc tggggaatga acttcatcaa acccgaggtg gcaccccggt
     4261 agtacagggt ctgttcatgc acatggccac caaagacata acgaatttcc gggttggccg
     4321 tggcggcatc cagactggct gtggcggccc gctcgtcata cacatagcgc cacagctcag
     4381 gaccgtcagc gctggcgtgg accagcaaaa tgttatcgac ttgtgtcgtc agggggagct
     4441 gttccagcca gtcacgctgc gccttgttta gttgccggct ggtccaggtc gccgtgctgt
     4501 cgccaatggt tttgatttcg gctggcgggt ggatggccat ggcgtcgtgg tttcccttga
     4561 ggacgatggc gccttcttcg gtcatcagca tcacgcggtc cacgactgct gcggggtcgg
     4621 ccccatagcc gaccaggtca cctaaaaaag caaagcgttg cgcccgctgg gcacgggcat
     4681 gggccaggca cgcgtcaagt gcctggatat tgccgtggat gtccgacagc agggccagtc
     4741 tcatggtgtc tccgaaatct gtataccgtc atcatgctac gaacggctga cagcaagttt
     4801 gcagactggc atgccggtct ggattgacgc aagtcaacag caaatcaaat acgcaacacc
     4861 cgtggccggg aagcggaccg ggacagctcg ggtcgctcag gcgaccggca acgacagcat
     4921 atgtcccatg cgcggatcgc cgcgacccgc cagaacggtc tggtaggccg attgcaccgc
     4981 ctgcgcgccg tcatggtgct ggacaacaac ccagggcggt gtggattgtg tcaccgcggc
     5041 cagaaatcct tcccatgact gcaccagctt ttggcccagc cccgcagcgc cccattcggt
     5101 actgcgtttc ttgatctggg cgggggcaaa gaacagcgtg ggtttggggc cgggcaggtc
     5161 acgaccgccc cccagttctg ccacatgcgt gccgccaatg gaactgctgt aggccagacc
     5221 cttgcagtgg gtgtgcagcg ttttgcggaa cgctgcattg ccggcaaagt cgacgtagat
     5281 gcagggcgtg tcctctgcga tgctggtgag ggcgtcgtag gtcagtaccc ggtggtagca
     5341 gcccaggctc tcacagaacg ccacgttgcc gggagaggtc agccccacca cttcaatgtt
     5401 cttgcgctgg gccatctgga acgctgtgcc caacgcggtc ttgctcgatg cactggacaa
     5461 cagcatcacg gcaggccgtc ccggtgtggc ggctgtacca aaccagtcgt tgtcggccag
     5521 aaaatcgtca atcagccagg acgtgatgaa cagcggacgc agcagcgcct gcaaatcttc
     5581 ggtatcggcg cggtagaacg ggtcggtgcg ggtacgggtg tactggttgt acaccgcatg
     5641 cagttctgcg cggtggggtg ccgcgtcgga aaacgacagg ggtgtcaccc gcgtcggggt
     5701 caggtctact gcattggcca tcggaaagta gccgtacaga tgctcaccca ccggcacatc
     5761 ggcgtggcgc gactgcacca cggtgccaaa cccccacacg ggaatgcgtc cccaagctgc
     5821 gtccggtgtt ccgtcttcgg acagcaccgg gaagaagcgc cagtaattca tggcgtcccc
     5881 aaaggcggcg taggtgatgt tgttggacgt gagggcaaag cgggtgatct gcacccggac
     5941 ctggccgtcc gccagggcgg cggcgggacg ggtccaaagg cgggtggtcg tgagctggtc
     6001 cttgcggacg agaaggtcag tggtgtgcat ggggtgacca taaaggaaac ggccagtccc
     6061 cacaacaggg actggccgca caggtgacag tcggtggact gccagagtgg cagtctggcg
     6121 atcagacgat ggtgatggtc acttcgatgt tgccgcgggt ggcgttggag taggggcaca
     6181 cagcgtgggc ctgttgcacc agagcttctg cagcagcgcg ctccatgccg gggatgttca
     6241 cagccagttg cacttcaata ccaaagcctt gcggaatctg gccaatgccc acggtggcat
     6301 cgatgctggt atcggcaggc agggcaatct tctgggtacc agcgacaaac ttcattgcgc
     6361 caatgaagca cgcgctgtag cctgcggcaa acagctgctc gggattgacg ccaccgccgg
     6421 cgccgcccat ttccttaggc actgccagct tgaggtcgag caggccgtcg gaggtgcgag
     6481 tggtgccgtc gcggccgcca gtggatttgg cgtgggcgcg gtacacaact ttttcgattt
     6541 tgttagccat ggtgtttcct tcgggggtgg gtgcccacgc aatgtgggcg ggttaaaaat
     6601 ggggtgggcg gtgtggcgta ctacgtaccg gtcagccggt tacgtagcgc ttgcacttcg
     6661 tgtgtcagcg tcatcaattc ggaaatgggg caacccgctg cctccaccac gcaggcgggc
     6721 acgctggtgg cgcgggtctt gagctttttg ccatcagacg tcaggcggac ttccacccgg
     6781 cgttcgtctt ccgccgaccg ctgacgagtc accaggcccg aatgctccat gcgcttgagc
     6841 agcggtgtca gcgtgccgga gtccagaaac aggcgctcgc ccagtgcaga gacggtcagt
     6901 ccgtcctgct cccacagcac cagcatcacc aggtattgcg ggtaggtcag ccccaggtct
     6961 tcgagcaggg gcttgtagag cttggtcatc gccagagacg ccgaatacaa ggcgaagcac
     7021 aactggttgt ccagcagcaa ggcagaggcg ggcagtgggc gggtggatgt acttggcatg
     7081 gatgaattgt agcgagaaat taaattgtgt acaattgaat tgggaaaatg ccactacaac
     7141 accagggtct ttgtagtggc aggggcggta gttcgcccgc ttgtctggct agcggcggaa
     7201 gcgtttgcgg gtgagtgcca gggcgaccca gaacgccacc gtggcgtagg ccaccaggac
     7261 cagggcgtgg cgcagcacat gggcaggcca ctggtccatg aagagcgggc gcaccagcgc
     7321 caccgcattg ctcagcggca accagtcggc caccacccgt accgcgacgg gcagctggtc
     7381 cagcgggaag aagatgccac tgaggaacat catcggcgtg aggaacagcg tgaagtagta
     7441 ggtaaaaaag tcgtagccct tggcgagcgc attgaacacc agcgccatgc agctgaaggt
     7501 aatgcccacg cccagcagaa tgggccaggc cagcagcagc ttggggctgt ggctgatgcc
     7561 cagtgccagc atgacgccca gaatggcggt cacggtaaac agcgatttga aagccgccca
     7621 cagcatctcg gccatcacga tgtcatcgag ccccacgggg gcattcatga tgccgtccca
     7681 ggttttctgc acatgcatgc gcgagaacgc ggagtacagg gcttcgaacg atgcggcctg
     7741 catggcgctc atgcaaatcg aaccgctggc cagaaacagg atgtagggca cttgcgcccc
     7801 atccaccgta atctgaccca ccaaggcgcc catgccgtag ccaaaggcta ccagccacat
     7861 cagcggttcg gcaatattgc ccaccagact gggaatggcc agtttgcgcc acaccagcag
     7921 gttgcgcagg aatacgggcc aaaagcgcag ggaaagccgt ggcgcacgcc acaccgattc
     7981 gtttgtcatg cgtcctcccg gatctggcga ccggtcaatt tgagaaacag gtcctccagg
     8041 ttggcgggcc ggtgcagcgt tctgagttgc gtatgcgcgc ccagagcgga cagcagggag
     8101 gcggcgtcgg atgtgtagaa gaacacggtc tctccgctga cttccacgcg cgctgccatg
     8161 ttgcgtagcg ggctgtccac cagcgccagt gcgccatttc cgtacacctc caccacgtcg
     8221 ggttccaggt gccgtgcaat caggtcacgc ggcttccctt ccgcaatctt gcggccatgg
     8281 tccagcacca gcaggcgggt gcacaggcgc tcggcctcgt ccatgaaatg ggtggtgagc
     8341 aggatcgatt tgccctgttg cagcaacaac tgcaggcgct cccacatcag gtggcgggcc
     8401 tgcgggtcca gcccggtggt cggctcgtcc agtagcaaca gtcgcgggtc gttgaccagg
     8461 gcgcgggcca gcgacaggcg gcgcttcatg ccgccggaca gctcgccggg tttggcgctg
     8521 gctttgctcg tcagcgacgc aaattccagc agctgcggaa tgcgggcgcg gatcgccgcg
     8581 tctttcatgc cgaaataacg cccatagaca agcaggtttt ccgcacaggt gaaatcgggg
     8641 tcgagggtgt cgaactggct caccacgccc agctgggcct tgatggccaa tgcgtcgcgc
     8701 ggcatgtgca gccccatcac ctcgacactt ccgttgtcgg gtgctgtcag tcccaggcac
     8761 atgcggatgg tggtggtctt gccagcgccg ttggggccga tcacgcccag gcactcgccc
     8821 ggttcgatct cgaacgacag gtctttgacg acttcggcgt caccatagcg tttgtgcaaa
     8881 tgctgcacac gaaaaagcgg tgtggacatg cattccattg tggcgcaacc cgctccgtcc
     8941 tgcatcagga agtcagctcc aggcggccac gtcccagtgc cttggcctgg tacaagccgt
     9001 tgtcggcccg gatgatgagt tcggtgaggc tatggtccga ggggcgcagc atcgccaggc
     9061 ccgcgctgta gttggccgcg ccctgcggga actcctggcc agctcgcaag aacgcctcgc
     9121 gcaggcggtg ctcaaacagc acggctgccg tcatatcggt ttgtgggagc agcagggcga
     9181 attcctcacc acccaagcgg gccgccaggt cgctgctgcg ccggttggac tgcagcacac
     9241 ggcccaccat gcgcaagacc tggtcgccgg cctcatgtcc atgggtgtcg ttgatgcgct
     9301 tgaaatggtc caggtcgagc atgatcagcg ccagcgggct gtcgtgccgt tgggcctgat
     9361 cgaacaacac cctggcacgc tcatcccagc cgtggcgatt gggcaaaccg gtcaggtggt
     9421 cggtgaaggc ctgcgcacgc aatttggctt cggcttcttc gcgccaggcc accagaatgg
     9481 ccaccgtaat gagaatctgc cccacgttgg ccacaatttg ggcgaccatg ttgactgggt
     9541 gacctgactg gaaatggggg taggactccg ggaagaaggc accgagaatg cccctgccga
     9601 tcgtggcgcc tgctactgcc ccgtaacaca ggaaaagcat ccagcgccag cgacggctgg
     9661 agtccacttt gtgccggatg gtggctcggg ccaccagcag cagctgggcc gccagcagga
     9721 agttggccca ccctacccgc acgctgtagt tggaaaagct caatgcatag cccagcggca
     9781 tcagtatgac cagagcaatc agcagcttct ccccaacgcg cctgccaagc catgcacgca
     9841 gggcaagaaa catcaggtag ttggaggcgc ccgcacaggc catgctgacc gtggagatgg
     9901 ccatatccag ccagtgccct gcccagaacc ccgaagcaat gatcgccacc caacccagag
     9961 tctgtgcgat cagactggcc tgggccagcc gcgcagcgcg gctgacgcca cgccccatga
    10021 tcagtggcag cgcgaccgac acggtgccca gattgagcgc tgtcagcaac aggaccgtaa
    10081 aaacgctgac ctggtccatg ggctacaacg gcgagttcac tgaaatgcca cttcggcaaa
    10141 actgcgcagc ttgcggctgt gcagttggtc gatgccttgc ttgcgcaaca actccacggc
    10201 gcgcatacca atgcgcagat gctggtccac gcgctcgcgg tagaagtggt tggccatgcc
    10261 gggcaacttg atttcgccgt gtagcggctt gtcgctcacg cacagcaggg tgccataagg
    10321 cacacggaag cgaaagccgt tggcagcgat ggtggcactc tccatgtcca gcgccacggc
    10381 gcggctctgg ctgaagcggc gttgcggctg gttgttgggc agcaactccc agttgcggtt
    10441 gtcggtgctg gccacggtgc cggtgcgcat gatgcttttc a
//
LOCUS       DAOSJM010000033        10313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6327_length_10313_cov_4.235329, whole genome shotgun sequence.
ACCESSION   DAOSJM010000033 DAOSJM010000000
VERSION     DAOSJM010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10313
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6327_length_10313_cov_4.235329"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="PKX82_02155"
     CDS             <1..617
                     /locus_tag="PKX82_02155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HOF51078.1"
                     /translation="TLANMINIATKPRWALGMAGTSRRQFGNIVGHVKGVTDMGNLAA
                     WTAQQFDPALNWGDVEWIKKRWGGKLILKGIQDVEDAQLAVNSGADALIVSNHGGRQL
                     DGAESSIRALPQIVDAVGGKIEVHMDGGIRSGQDVLKARALGAQGTYIGRAFLYGLGA
                     MGEEGVSKCLEIIHRELDLTMAFCGHTQIGTVDKGVLLPGTFPT"
     gene            644..1186
                     /locus_tag="PKX82_02160"
     CDS             644..1186
                     /locus_tag="PKX82_02160"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOF51079.1"
                     /translation="MPQFENTAPSPSPTSSPTSRGVSGWWRALSIALLIVLGLGLASA
                     ISMYEQFKAQLEHMQTQLQTVPQIKTISVLADKEHAPAILVTQDPQDTTLQLQRLNSV
                     VEGPEDSMQLWALSGDNRPVSLGVLSPKLTMQRLNATDKDLDTMDRLAISVENRGGVS
                     PLQGPRLPYLYQGAIVRKAR"
     gene            complement(1183..4578)
                     /locus_tag="PKX82_02165"
     CDS             complement(1183..4578)
                     /locus_tag="PKX82_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF51080.1"
                     /translation="MQSGFFRPALPLQQRIRWLAIAALVLLWGAAAVVGYSNYKNAHE
                     QMDRQTSVLAATLAQQTSATFRLADHVMSGIRSEWLNTPVRTGWQVPPQHSAYLRDLM
                     AHISIVDANGQEVLSTLDADRSPVAWADRYYFNTHKNSGRDEVLVSSPFKDALTGKWH
                     IDVSRPLWERGAFVGVMVLSIEPEYFSRFFKTVGLGKQGLVSLVSDNGTMMARSLDPG
                     RFIGGVIRSAPFLVPGADIAGHFHHASPLDGVARSYGYVRIPDYQVTLVAAVGIDEQM
                     APLTQQRLVFMGIAICVTLLLILTARSLMRSVGRRETAENQLREAQSRYQGLVESLPV
                     GVVVHRNGKFLYVNPAAVAILGARSADALLGQPILEIVHPDFREAAVARIKFSLETGQ
                     AMPPYEEKFLKIDGTVIDVEVRGNAITFEGEPASQVSFQDITERKKVEQALHASEEKW
                     RSIIANSVNGISIFSVHGLLEKSSQGMLKMFGYANEEEILGRPLDDFADTASLARIKA
                     VVADLAQGGYVGALEGWAIRKDGTRFLAEAQVDVLHNAQGEATQIIFMVRDISEHKRS
                     QEELLLAASVFTHAREGIMITDAAGTIVAVNETFSHITGYHRREVLGQSPRILNSGRQ
                     GDEFYETMWKAIVDAGHWSGEVWNRRKNGEVYAEMLTISVVLDDEHKPQNYVSLFTDI
                     TPMKEYQSQLERIAHYDALTNLPNRVLFADRLRQAVVHSQRRKRLLSVVYLDLDGFKA
                     VNDAHGHAVGDELLIVVAQRMKIALRDGDSLARVGGDEFVAVLVDLDRPADCEPVLGR
                     LLAAASGPVTVQTATGPVNLQVSASIGATIYPRDGSDADLLMRHADQAMYLAKQAGKN
                     RFHLFDTDRDAAVKTQRESVERVRKALECNELVLHYQPKVNMLTRDVVGAEALVRWQH
                     PERGLLAPAEFLPIIENHPLSVAMGEWVMNTALTQIAVWKALGLDLPVSVNMGAYQLQ
                     QPNFVENLKASLARHPLVPRFRLELEILETSAIEDLAQVSGILRACRAEGVLFSVDDF
                     GTGYSSLTYLKHLPAETLKIDQSFVRGMLSDPDDMAIVSGVIGLAKAFGRKVIAEGVE
                     TDAHGEKLIALGCPVAQGYGIARPMPAEQMVQWVQRWKSLATA"
     gene            4651..5886
                     /gene="dinB"
                     /locus_tag="PKX82_02170"
     CDS             4651..5886
                     /gene="dinB"
                     /locus_tag="PKX82_02170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOF51081.1"
                     /translation="MPSPASPARRIAHLDMDAFYASVELLRYPQLKGLPVVIGGGRRR
                     EDDALREARGDTPLHQIPVSEFPVLSGYTGRGVITTATYAARQFGVGSAMGLMKAAKL
                     CPQAILLPVDFAQYRHYSARFKAIITDIAPVMENRGVDEVYIDFTDVPGGQREGGRVL
                     ARLIQKAIFDDTGLTCSVGVAPNKLLAKMASEFKKPNGIAIVHESDLETMIWPLACRK
                     INGIGPKADEKLKRHGIETIGQLAARELQWLIDTFGQRTGAWLHAASHGQDDRPVETE
                     SEPVSMSRETTFERDLHAVRDKAELGAIFTQLCEQVAADLQRKGYEGKTIGIKLRFED
                     FKGVTRDLTLDHCTADAQEIRRAAGLALKRAPLDKRLRLLGVRVGSLQKQGLAPAPSA
                     QEAINSVANALQAETGQLF"
     gene            6003..7406
                     /locus_tag="PKX82_02175"
     CDS             6003..7406
                     /locus_tag="PKX82_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOF51082.1"
                     /translation="MPRVTPQTELDQLVDLISPHPYGMGIEAIARVLGDSLQRRTLQR
                     RLAALVAQGRIQMLGEARSVRYRIPGYPPAPRAVAASVQEPAEAYVPTSPQGEEIKAY
                     VRQPRALRAPVGYKLEFLEQYHPNHTAYLPQALRDQLHTLGRAFGQSPADQTPAGTFA
                     RDILNRLLIDLSWASSQLEGNTYSRLDTQRLIEFGQAADGKNALETQMILNHKQAIEY
                     LVLDPAHAQVNPDTVIALHAFLSDGLMADPAAVGRVRRRAVEIGGSVYLPVALPQRLE
                     ELFGIVIQMAAEIADPFEQAFFLMVHLPYLQPFEDVNKRVSRLAANIPFIRHNLCPLS
                     FIDVPQQAYVDAMIGVYELNRIELLRDVFVWAYARSCQQYVAVQQNLVPPDILRLRYR
                     QALGEVVAAIVRNGEAPTQAAVQARVPPTVAAPDRAYFIQLVLQEFTALHPGNAVRFG
                     IRPLELAAWTEKHRGAQ"
     gene            complement(7354..8385)
                     /locus_tag="PKX82_02180"
     CDS             complement(7354..8385)
                     /locus_tag="PKX82_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOF51083.1"
                     /translation="MSCEIPARPATILLAPMEGLLDFVLRDILTRVGGIDRCVSEFIR
                     ISDQLLPDRVFQRIVPELHNGGRTLAGVPVRAQLLGSDPVCMAENAARLAALGPHGID
                     LNFGCPAKVVNRHGGGAKLLEEPEVVAAIVRAVRRAVPADMPVSAKMRLGYMDDARAV
                     ENAVAIAESGASELVVHARTKAQAYTPPAYWDRIADIRAAVNIPVIANGEIWTVADAQ
                     ACMAESGCTSVMLGRGAVVDPGLALAIRGTAGGSTQAVTWVTLLPLMADFWHIVCTQL
                     DPKKRAGRLKQWLNFLRRRFPEAEVAYQALRTARDKDVDVWLAAELQRHGVIAHPDVF
                     LSTPQALAA"
     gene            8451..8771
                     /locus_tag="PKX82_02185"
     CDS             8451..8771
                     /locus_tag="PKX82_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51084.1"
                     /translation="MITWLAPAISLTELHRIKRWQVDHALEHPVEYQTWDAVLTTWFM
                     GWVGWLPTWALDLWWCSVPCLLGMLAPRLYIGWRARAHAQHRLRCDWLPEVRGHAGES
                     TMNH"
     gene            complement(8798..9007)
                     /locus_tag="PKX82_02190"
     CDS             complement(8798..9007)
                     /locus_tag="PKX82_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51085.1"
                     /translation="MTPQQFIAKWGPGGPAFELNERQGAQPHFIDLCQLLGEARGYTA
                     DMPDEEILKRLLALNLERAGGAGCP"
     gene            complement(9032..9883)
                     /gene="queF"
                     /locus_tag="PKX82_02195"
     CDS             complement(9032..9883)
                     /gene="queF"
                     /locus_tag="PKX82_02195"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913989.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HOF51086.1"
                     /translation="MNTPEQSQLGKASAYIDQYDASLLFPIPRAGKRAEIGIAGAPPF
                     LGGDLWTAFELSWLNLRGKPQVALAHFVVPCESVNIVESKSFKLYLNSFNNTRFADAA
                     EVQARLRADITEAVWRGATVQSSVGVKLIAPEKFDMEPVHELDGLSLDRLDVECTQYQ
                     PAPELLVVESVDEEAPVTEVLVSNLLKSNCLVTGQPDWGSVQISYSGLPINQEGLLKY
                     LVSFRNHNEFHEQCVERIFMDIWTRCKPVKLAVYARYTRRGGLDINPFRTSYPQPLPG
                     NVRTARQ"
     gene            complement(9888..>10313)
                     /locus_tag="PKX82_02200"
     CDS             complement(9888..>10313)
                     /locus_tag="PKX82_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOF51087.1"
                     /translation="QAMISRPNGTSAQAVVDHTVRLFKAIGSPAYPTPEDELRARVLE
                     GIHRSFHPVGTLRQMVAIVSDITRAAQLLRITSPTLVIHGKADPLVPYGCGEDTARRI
                     PGAKLVGIDGMGHDLPPEPVTQILAAMVPHLNAATPKAA"
BASE COUNT         1888 a         3418 c         3150 g         1857 t
ORIGIN      
        1 tcacccttgc caacatgatc aacatcgcca ccaagccacg ctgggcgctg ggcatggcgg
       61 gcacttcacg ccggcagttc ggcaacatcg tgggccacgt caagggcgtg accgacatgg
      121 gcaatctggc cgcctggacc gcacagcagt ttgacccggc cctgaactgg ggcgatgtgg
      181 aatggatcaa gaagcgctgg ggtggcaagc tcatcctcaa gggcattcag gatgtggaag
      241 acgcacagct ggcagtcaac tccggtgccg atgcgctgat cgtctccaac cacggcgggc
      301 gccagctcga tggcgccgag tcctccatcc gcgccctgcc gcagattgtg gatgccgtgg
      361 gcggcaagat cgaggtgcac atggacggcg gcatccgcag cggccaggac gtgctcaagg
      421 cgcgcgcgct gggtgcccag ggcacctaca ttggccgcgc attcctgtat ggcctgggcg
      481 ccatgggcga ggaaggcgtg agcaagtgcc tggaaatcat ccaccgggaa ctggacctga
      541 ccatggcctt ctgtggccac acccaaatcg ggacggtgga caagggcgtg ctgctgccag
      601 gcacctttcc gacctgatgc gttggggtag gatgcgcgca tctatgcccc aattcgaaaa
      661 caccgctccc tccccctccc cgacctcttc tcccaccagc cgcggtgtca gcggctggtg
      721 gcgcgcgctc agtatcgcct tgctcatcgt cctgggcttg gggctggcat cggccataag
      781 catgtacgag caattcaagg cccagctcga acacatgcag acccagttgc agacggtacc
      841 ccagatcaag accatttcgg tgctggccga caaggaacac gcaccggcca tcctggtgac
      901 ccaggacccg caggacacca cgctgcagct gcagcgcctc aatagcgtgg tggaaggccc
      961 ggaagacagc atgcagctct gggccctgtc tggtgacaac cgtccggtgt cgctgggcgt
     1021 gctcagcccc aagctcacga tgcagcgcct caacgccacc gacaaggacc tcgacaccat
     1081 ggaccgcctg gccatcagtg tggagaaccg tggtggtgtg tcccccctgc aggggccacg
     1141 cctgccctat ctgtaccagg gcgccatcgt gcgcaaggcg cgctaggccg tcgccaggct
     1201 cttccagcgc tggacccact gcaccatctg ctcggccggc atgggccggg caatcccgta
     1261 gccctgcgcc accgggcaac ccaaggcgat gagcttttcg ccatgggcat cggtttcaac
     1321 cccttccgca atcaccttgc ggccaaacgc cttggccagc ccgatcacgc cactgacgat
     1381 ggccatgtcg tcggggtcgg acagcatgcc ccgcacaaaa ctctggtcga tcttgagggt
     1441 ctccgccggc aggtgcttga ggtaggtcag cgaggaataa ccggtaccaa aatcgtccac
     1501 cgagaacagc accccttcgg cgcggcaggc ccgcaaaatg cccgagacct gtgccagatc
     1561 ctcgatcgcg ctggtctcca gaatctccag ctccagcctg aaccggggca ccagcgggtg
     1621 gcgtgccaga ctggccttga ggttctccac aaaattgggc tgctgcagct ggtaggcgcc
     1681 catattgaca ctcacgggca agtccagccc cagagccttc catacggcaa tctgcgtcag
     1741 agccgtgttc atcacccact cgcccatcgc cacactgagc gggtggttct cgatgatggg
     1801 cagaaactcc gccggcgcca gcagcccgcg ctcgggatgc tgccagcgca ccagcgcttc
     1861 ggcgcccacc acgtcgcggg tcagcatatt caccttgggc tggtaatgca gcaccagctc
     1921 attgcattcg agcgccttgc gcacacgctc cacactctcg cgctgggtct tgaccgctgc
     1981 atcccggtcg gtatcgaaca gatggaaccg gtttttgccc gcctgcttgg ccaggtacat
     2041 ggcctggtct gcatggcgca tcagcaagtc agcgtctgac ccgtcgcggg gataaatggt
     2101 ggccccgata ctggccgata cctgcaggtt gaccggcccg gtggcggtct gcacggtgac
     2161 cggacccgag gcggccgcca gcaaacgccc cagcaccggt tcgcaatctg cgggccggtc
     2221 caggtccacc agcaccgcca cgaactcatc gccccccaca cgcgccaggg agtcgccatc
     2281 gcgcagtgca atcttcatgc gctgggccac cacaatcagc agctcgtcgc ccacggcatg
     2341 tccgtgcgca tcgttgacgg ccttgaaacc atccagatcg aggtacacca cactgagcag
     2401 gcgcttgcgc cgctggctgt gcaccaccgc ctggcgcagc cggtcggcaa acagcacgcg
     2461 attgggcagg tttgtcagcg cgtcgtagtg ggcaatgcgc tcaagctggc tctggtattc
     2521 cttcatcggc gtgatgtcgg tgaacagcga cacatagttc tgcggcttgt gctcatcgtc
     2581 cagcaccacg ctgatggtga gcatctcggc atacacctcg ccgttcttgc ggcggttcca
     2641 cacttcaccc gaccaatggc cggcatccac aatcgccttc cacatggttt cgtaaaactc
     2701 atccccctgg cgaccggaat tgagaatgcg cgggctttgt cccagcacct cccgccggtg
     2761 gtagccggtg atgtggctga aggtctcgtt caccgcgacg atggtgcctg ctgcatcggt
     2821 aatcatgatg ccctcgcgcg catgggtgaa cacgctggcg gccagcagca gctcttcctg
     2881 cgagcgcttg tgttccgaaa tgtcgcgcac catgaaaatg atctgcgtgg cctcgccctg
     2941 ggcgttgtgc agcacatcga cctgggcctc ggccaggaaa cgggtaccgt ctttgcggat
     3001 cgcccacccc tccagggctc ccacatatcc gccctgggca aggtctgcca ccactgcctt
     3061 gatccgcgcc aggctggcag tgtccgcaaa gtcgtccagt ggcctgccca ggatttcctc
     3121 ttcgttcgca tagccaaaca tcttcagcat gccttgggac gacttttcca gtagcccgtg
     3181 gacactgaaa atgctgatgc cgttgaccga attggcaatg atggagcgcc acttctcctc
     3241 gctggcgtgc agcgcctgct ctactttctt gcgctcggta atgtcctgga agctgacctg
     3301 tgacgcaggt tcgccctcga acgtgatggc atttccccgg acttccacgt caatcacggt
     3361 gccgtcgatt ttgagaaatt tttcctcata cggcggcatg gcctgcccgg tttccaggct
     3421 gaacttgata cgggcgacgg cagcctcccg aaagtccggg tgcacgatct ccagtatcgg
     3481 ctggcccagc aatgcgtctg ctgaccgggc cccaagtatt gccaccgcag ccgggttgac
     3541 gtacagaaat ttcccgttgc ggtgcaccac cacacccacg ggcaggcttt ccaccagccc
     3601 ctggtagcgg gactgcgctt cacgcaactg gttttctgcc gtttcccttc gtcccacgct
     3661 gcgcatgagg ctgcgtgcag tcaggatcag cagcagtgtc acgcaaatgg caatgcccat
     3721 gaacaccagg cgctgctggg tcagtggcgc catctgctcg tcaatgccca ccgcggccac
     3781 cagcgtcacc tggtaatctg gaatgcgcac atagccatag ctccgggcca cgccatccaa
     3841 gggcgatgca tggtgaaaat gcccggcaat atcagcaccc ggcaccagga atggcgcgct
     3901 gcggatcact cccccgatga accgtccggg gtccagggag cgcgccatca tcgtcccgtt
     3961 gtccgacacc agggaaacca gcccctgctt gcccaggccc accgttttga aaaaacggga
     4021 aaagtactcg ggctcgatcg acagcaccat gacaccgaca aatgcgccgc gctcccacaa
     4081 cgggcgcgac acatcgatgt gccacttgcc ggtgagggcg tccttgaacg gcgaactgac
     4141 cagcacctcg tccctgccgc tgttcttgtg ggtgttgaag taataccgat cagcccaggc
     4201 gacaggcgac ctgtctgcat ccagcgtgct cagcacttcc tggccattgg catccacaat
     4261 gctgatgtgc gccatcaaat cccgcaggta ggccgagtgc tgcggcggca cctgccaccc
     4321 ggtccgcacg ggggtattga gccattcgct gcgaatgccg ctcatgacat ggtccgccag
     4381 gcgaaacgtg gcgctggttt gctgcgccag cgtcgctgcc agcaccgagg tctgccggtc
     4441 catctgctcg tgggcgtttt tgtagttgct gtaccccacc acagcggcgg cgccccacaa
     4501 caacacgagg gctgcgatcg ccagccaccg gattctttgc tgcaggggca gcgccggtct
     4561 gaaaaatccg gattgcatgg aaccgccttg tcaccgctgt ataagtattc acgaattatc
     4621 agcctcccgg cagcttgcta tcctagggat atgccctcac ccgcgagtcc cgcccgccgc
     4681 attgcccacc tcgacatgga tgcgttttac gcctccgtcg agttgctgcg ctatccgcag
     4741 ctcaagggcc tgccggtggt gattggcggc ggccgccggc gcgaggacga cgccctgcgc
     4801 gaagcccggg gcgatacacc cttgcaccag ataccggtca gcgaatttcc ggtcttgtcc
     4861 ggctacaccg ggcgcggcgt catcaccacc gccacctacg cggcgcgcca gtttggcgtg
     4921 ggctcggcca tggggctgat gaaggccgcc aagctgtgcc cgcaggccat cctgctgccg
     4981 gtggattttg cacagtaccg gcactactcg gcacgcttca aggccatcat caccgacatt
     5041 gccccggtca tggaaaaccg cggggtggac gaggtgtaca tcgacttcac tgatgtgccc
     5101 ggtggccagc gcgaaggcgg ccgggtgctg gcgcggctga tccagaaagc catttttgac
     5161 gacaccggcc tgacctgctc ggtgggcgtg gcccccaaca aactgctggc caagatggcc
     5221 agcgagttca agaagcccaa cggcatcgcc atcgtgcacg agagcgacct ggagacgatg
     5281 atctggccgc tggcctgccg caagatcaac ggcattggcc ccaaggcgga cgaaaaactc
     5341 aagcgccacg gcatcgaaac catcggtcag ctcgccgcgc gcgagctgca gtggctcatc
     5401 gacacctttg gccagcgcac cggtgcctgg ctgcatgccg ccagccacgg tcaggacgac
     5461 cgaccggtag aaactgaaag cgagccagtc agcatgagcc gcgaaaccac cttcgagcgt
     5521 gacctgcacg cggtgcgcga caaggccgag ctcggagcca tcttcaccca actgtgcgaa
     5581 caggtggccg ccgatctgca gcgcaaaggc tacgagggca agacgattgg catcaagctg
     5641 cgttttgagg acttcaaggg cgtaacgcgc gacctgaccc tggaccactg cacagctgac
     5701 gcgcaggaaa tccgccgcgc ggccggcctg gccctcaagc gcgccccgct ggacaaacgg
     5761 ctgcggctgc tcggtgtccg cgtgggttca ttgcaaaaac agggcctggc ccccgcccct
     5821 tctgcgcagg aagctatcaa ttcagtagca aatgccttac aggccgaaac cggtcagctg
     5881 ttctgaacac tctgtagata tgtcgtgatt tttcaataac gacattttat atgtcgcaat
     5941 aatacttgcg cgacacttaa agtgacgcga tactgcggtt ctgaaaaccg agaaccaagc
     6001 acatgccacg cgtcaccccc caaaccgagc tggatcaact ggtagatttg atctctccac
     6061 acccgtacgg catgggcatt gaggccatcg cccgggtgct gggcgacagt ttgcagcgcc
     6121 gcaccttgca gcgccgattg gctgcgttgg tcgcgcaagg gcgtattcag atgctgggcg
     6181 aggcgcgatc tgtgcgctac cggatacccg gctacccccc tgccccacgc gctgttgcag
     6241 catcggtgca agagcccgcc gaagcttatg tccccacttc gccgcagggc gaggagatca
     6301 aagcctatgt gcgccagccg cgtgcgttgc gcgcgccggt gggctacaag ctggaatttc
     6361 tggagcaata ccaccccaac cacacggcct atctgccgca ggccttgcgg gatcagttgc
     6421 acacattggg gcgcgcattc ggccaatctc ccgccgacca gacacccgcg ggcacctttg
     6481 cgcgcgacat cctcaatcgc ttgttgattg acctgtcctg ggcctcctca caactggagg
     6541 gcaacaccta cagccggctg gacacgcagc ggctgatcga gttcggccag gcggccgatg
     6601 gcaagaatgc gctggaaacc cagatgatcc tgaaccacaa gcaggccatt gaatacctgg
     6661 tgctcgatcc cgcacatgcc caggtcaacc ccgacacggt catcgcactg catgcttttc
     6721 tgtccgatgg tttgatggcc gacccagccg cagtgggccg cgtgcgccgc cgggcggtcg
     6781 agatcggcgg cagtgtgtac ttacccgtcg ccttgccaca gcggctggaa gaactgtttg
     6841 gcatcgtcat ccagatggct gcggaaattg ccgacccctt cgagcaggca tttttcctga
     6901 tggtgcacct gccctacctg caaccgtttg aagatgtgaa caagcgcgta tccaggctgg
     6961 cggccaacat acccttcatt cgccacaacc tgtgcccgct gtcgttcatc gacgtgccgc
     7021 agcaagccta tgtggacgcc atgatcggcg tgtacgaact caaccgcatc gaacttctgc
     7081 gcgacgtgtt cgtctgggcc tacgcgcgct cctgccagca atacgtggcg gtccagcaga
     7141 acctggttcc acccgatatt cttcgtctgc gctaccgcca ggccctgggc gaagtggtcg
     7201 cggccatcgt gcgcaacggc gaggccccga cgcaagctgc ggtgcaagcc agggtgccac
     7261 ccacggtggc agcgcccgac agggcctatt tcatccagct ggtgctgcag gagttcaccg
     7321 ccctgcatcc cgggaacgcg gtgcgctttg gcatcaggcc gctagagctt gcggcgtgga
     7381 cagaaaaaca tcggggtgcg caataacgcc gtggcgctgc aactccgccg ccagccacac
     7441 gtccacatcc ttgtcgcgtg cggtgcgcag cgcctggtac gccacctcgg cctccgggaa
     7501 gcggcgacgt aaaaagttga gccactgctt gagccggcca gcgcgcttct tggggtccag
     7561 ctgggtgcag acgatgtgcc agaaatccgc catcagcggc aacagcgtga cccaggtcac
     7621 ggcctgggtc gatccccctg cagtgccacg gatcgccagc gccagccccg ggtccaccac
     7681 cgcaccgcgc cccagcatca cgctggtaca gcccgactca gccatgcagg cctgcgcatc
     7741 ggccacggtc cagatttcgc cgttggcaat caccggaata ttgactgcgg cgcggatgtc
     7801 ggcaatgcgg tcccagtagg ccggcggtgt gtaggcctgc gccttggtgc gggcgtgcac
     7861 caccagttcg ctggcgccgc tctcggcgat agccaccgcg ttttccacgg cgcgcgcgtc
     7921 gtccatgtag cccaggcgca tcttggcgga cacgggcata tcggcaggca ccgcgcggcg
     7981 caccgcgcgc acgatggcag ccaccacctc cggctcttcc agcaatttgg cgccgccgcc
     8041 atggcggttg accactttgg ccgggcaacc aaagttcagg tcaatgccgt gcggccccag
     8101 cgcggccagc cgcgcggcgt tttcggccat gcacaccgga tcgctgccca gcagctgcgc
     8161 acgcaccggc accccggcca gcgtgcgccc gccgttgtgc agctcgggca cgatgcgctg
     8221 aaacacgcgg tcgggcagca gctggtcgga gatgcggatg aactcggaca cacaacggtc
     8281 aatgccgccc acgcgggtca ggatgtcgcg cagcacaaag tccagcaggc cctccatggg
     8341 cgcgaggagg atggtggcgg ggcgcgcagg aatttcacac gacatgtacc gttcgccttg
     8401 atttctactt acattgaact tacagtgaac attcgcactg ggagactgca atgatcacat
     8461 ggttagcccc cgccatttcg ctcaccgaat tgcaccgcat caaacgctgg caggtggacc
     8521 atgcgctgga gcaccctgtg gaataccaga cgtgggacgc ggtgctgacc acctggttca
     8581 tgggctgggt gggctggttg cccacctggg cgctcgacct ctggtggtgc agcgtgccgt
     8641 gcctgttggg tatgctggca ccgcggctct acattggctg gcgcgcccgt gcccacgccc
     8701 aacaccgctt gcgctgcgac tggctgccag aggtgcgggg acacgctggc gaatcaacaa
     8761 tgaaccatta ggtgggtgcc tgccggagag ctgcggtcta tgggcaacct gctccacccg
     8821 ccctctccag attcagcgcc agcaggcgtt tgaggatttc ttcgtcgggc atatcggcgg
     8881 tgtagccacg cgcctcgccc agcagctggc acaggtcgat gaagtgcggc tgcgcgccct
     8941 ggcgttcgtt cagctcgaac gccggcccgc ccgggcccca cttggcgatg aattgttggg
     9001 gggtcatggg ttgaggcgcg tccgggttgg gctactgccg cgcggtacgc acattacccg
     9061 gcaacggctg cgggtagctg gtgcgaaacg ggttgatgtc gagcccgccg cggcgggtgt
     9121 agcgggcgta caccgccagc ttgaccggtt tgcagcgcgt ccagatgtcc atgaagatgc
     9181 gctcgacgca ctgctcgtgg aactcgttgt ggttgcgaaa actcaccagg tatttcagca
     9241 ggccttcctg gttgatgggc agaccgctgt agctgatctg cacgctaccc cagtcgggct
     9301 ggccggtgac caggcaattg ctcttgagca ggttgctcac cagcacttcg gtgactggtg
     9361 cctcttcgtc cacggactcc accaccagca gctcgggcgc gggctggtac tgggtgcatt
     9421 ccacatcgag gcggtccagg ctcaggccgt cgagttcgtg caccggctcc atgtcgaact
     9481 tctcgggcgc aatgagcttg acgcccaccg acgactgcac cgtggcgccg cgccagacgg
     9541 cctcggtgat gtcggcgcgc aggcgcgcct gcacctcggc ggcatcggca aagcgggtgt
     9601 tgttgaagct gttgaggtag agcttgaagg acttgctctc gacgatgttg accgactcgc
     9661 agggcaccac aaagtgcgcc agggccacct gcggcttgcc gcgcaggttg agccaactca
     9721 gctcgaacgc ggtccacaaa tcgcccccca gaaacggcgg cgcaccggca atgccaatct
     9781 cggcacgctt gcccgcgcgg ggaatgggaa acagcagtga cgcgtcgtac tggtcgatgt
     9841 aggccgaagc cttgccgagc tgggattgtt cgggggtgtt catggtgtca ggccgccttg
     9901 ggagtggcgg cgttcaggtg gggcaccatc gcagccagga tttgcgtgac cggctcgggc
     9961 ggcaggtcgt gccccatgcc gtcgataccc accagcttgg cgccggggat gcgccgcgca
    10021 gtgtcttccc cacaaccgta aggcaccagc ggatcggcct tgccgtggat caccagcgta
    10081 gggctggtga tgcgcaggag ctgtgcggcg cgggtgatgt cggaaacgat ggccaccatc
    10141 tggcgcagtg tgcccaccgg gtggaaactg cggtggatgc cttccagcac gcgggcgcgc
    10201 agctcgtctt cgggagtcgg gtaagccgga ctgccaatgg ccttgaacaa gcgcacggtg
    10261 tggtccacca cggcctgcgc actggtgcca ttggggcggc tgatcatggc ctg
//
LOCUS       DAOSJM010000034        10294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6341_length_10294_cov_4.617834, whole genome shotgun sequence.
ACCESSION   DAOSJM010000034 DAOSJM010000000
VERSION     DAOSJM010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10294
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6341_length_10294_cov_4.617834"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKX82_02205"
     CDS             <1..311
                     /locus_tag="PKX82_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOF51088.1"
                     /translation="TFHAWYPGCPMEEDAYGIPKPKDTEVIVPTLLFAPCVGYGPGGY
                     RLGYGGGFYDRMLSSLEPRPFTVGLAFGTGFVEDFEPEPHDMPLDAILNDHGVVWPIN
                     "
     gene            314..1615
                     /locus_tag="PKX82_02210"
     CDS             314..1615
                     /locus_tag="PKX82_02210"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOF51089.1"
                     /translation="MSVTQDLLDPLLDATFKGFPHHSGPLRRSQIGAQGWNVLRGDLP
                     LPIALIKQDLLRHNLRWMQDFAASRGLGLAPHGKTSMSPQLFQMQMDAGAWGMTFATV
                     TQMRTGISAGVQRCLIANQVFTALDLQGIDAMRREHPGLRVVFLVDSAQQLALIKAWR
                     QKNPSAQPFEVLLEMGVDGGRTGCRTAEQALELAGRLHNSSAVRLVGVECYEGLAAQG
                     NTAQDQLYAQSLMDRVEAVARACDAQNLFDSDEVIVTAGGSAIFDLVAARLKPALRRP
                     VLGLLRSGCYVTHDQGTYQRMMRAVAERTGCTDGLEAAMEVWACVQSCPEPGLVILAA
                     GKRDLSYDWEMPRPLAVLPQSAVERDPAHLQPVGEDWKVVALNDQHAYLRGSGAAFEA
                     LGVGDLVGLGISHPCTTFDKWRWMPVVDASYTVCDAITTCF"
     gene            complement(1620..2504)
                     /locus_tag="PKX82_02215"
     CDS             complement(1620..2504)
                     /locus_tag="PKX82_02215"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HOF51090.1"
                     /translation="MSLFLRSCIRWFAIISVLASAMIMGGCSAVRLGYNNAPDLTYWW
                     LDSYLDFDTPQTLRVRADLQTWQDWHRKEELPQYAELLKGLQPLVNQTVSAEQVCSIY
                     TTIETRLLIATGRILPTAAAMAPLLQPSQLVHLERTWEKRNQEWREDWLDGTPAERAS
                     DRLKKFVERAESFYGRLSDGQAERMRALLEASPLEANTLYRERLRRQQDTLQTLQALR
                     SGNATEAQAQAELQALLNRSVHSPQAALRQYQDRMRQHSCNLLAVLHNSATPAQRTKL
                     SQVLQGYENDARALSAAR"
     gene            2503..3963
                     /gene="glmU"
                     /locus_tag="PKX82_02220"
     CDS             2503..3963
                     /gene="glmU"
                     /locus_tag="PKX82_02220"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOF51091.1"
                     /translation="MNVVSTQRNGEEGIHRNPVDVVIMAAGKGTRMKSKLPKVLHQLA
                     GRPLLQHVVDTAGELMARKVVVITGHGAIEVEAALAVNTGARAEFALQCVRQDPQLGT
                     GHAVQQAVPALDDDGIVVVLSGDVPLTQSTTLMHLIAACAGKSLALLTLEMPNPAGYG
                     RIVRAGGTRDGAVQAIVEHKDASDAQRQITEIYSGIMAVPAAQLKRWLARLDNKNAQS
                     EYYLTDIVKFAVADGVPVVGHKIADAVQVAGVNSPVQLAELERAYQLRQAHRLMEEGV
                     RLADPARIDVRGDLLCAHDVSIDVNCVFMGQVSLGEGVSIGANCVIANAVIEAGAVIH
                     PFTHIDGEKMGVTVGPGALIGPFARLRPGARLGAEVHIGNFVEVKNSTLAAGAKANHL
                     AYLGDATVGERVNYGAGSITANYDGANKHRTVIEADVHVGSNCVLVAPVTIGAGGTVG
                     GGSTITKSTEPGALSVARGRQISIAHWERPRKAPKS"
     gene            3976..4713
                     /locus_tag="PKX82_02225"
     CDS             3976..4713
                     /locus_tag="PKX82_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51092.1"
                     /translation="MDDASAQRIAQLEADLAAARREMQDFTYTVSHDLRAPLRHIISY
                     ARLAQEDAGPQLSGDVLGFLATVTDSARNMGAMLDALLELSRLGTVALDVESVPLQAM
                     VEDVALSLRNAHPQRAIDWRIELDACSVTGDASLLRQALVCVLDNAMKFTAPRAHAEV
                     AVSAHADAPAQRVVIAVQDNGVGFNPVLQDKLFQPFHRLHSSSQFSGLGLGLAQVRKI
                     VVRHGGQVAIAARVDGGCTVTLTLPLA"
     gene            complement(4710..5192)
                     /locus_tag="PKX82_02230"
     CDS             complement(4710..5192)
                     /locus_tag="PKX82_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOF51093.1"
                     /translation="MESIELDAIDLLLLDQLQRDASLSNQALAERVHTSPPTCLRRVK
                     RLHEAGLIERQVAVLNPERMAQTLGHGLTAIVEITLDRQDAGALEAFETRIAQDAAVQ
                     QCYRVSPGPDFCLVVHTRDMPAYQALTQRLFTVDANVRNVKAFFSVKRSKFGAELPLS
                     "
     gene            5330..7225
                     /gene="glmS"
                     /locus_tag="PKX82_02235"
     CDS             5330..7225
                     /gene="glmS"
                     /locus_tag="PKX82_02235"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOF51094.1"
                     /translation="MCGIVGAVSTRNIVPVLVQGLERLEYRGYDSCGVAVYAQDGTPG
                     LRRARSTSRVAELMEQVAAGSVQGTLGIAHTRWATHGAPAVHNAHPHFSHGPGADVSG
                     KPGRIALVHNGIIENHDELRAALQAKGYVFDSQTDTEVIAHLIDSLYDGDLFEAVKAA
                     TVQLHGAYAIAVFCKDEPHRLVGARAGSPLILGVGKDGQEHFLASDAMALAGVTDQIV
                     YLEEGDLVDVQLGKYWLFDKAHKAVAPAQRPVKTVHAHSGAAELGPYRHYMQKEIFEQ
                     PRAIADTLEGVEGIAPELFDGQMNHAPGANAARVFAQIDSVLILACGTSYYSGCTAKY
                     WIESIAKVPCQVEVASEYRYRDSVPNPNTLVVTITQSGETADTLAALRHAQSLGMKHT
                     LTICNVATSAMVRECSLAYVTRAGVEIGVASTKAFTTQLAGLFLLTLCLAQAKSRLSD
                     AEEARHLKAMRHLPTALQAVLALEPQVMAWAEDFARKENALFLGRGLHYPIALEGALK
                     LKEISYIHAEAYPAGELKHGPLALVTSEMPVVTVAPNDALLEKLKSNLHEVRARGGVL
                     YVLADGDTRIESGEGLHVIRMPEHYGELSPLLHVVPLQMLAYHTACARGTDVDKPRNL
                     AKSVTVE"
     gene            complement(7312..7953)
                     /locus_tag="PKX82_02240"
     CDS             complement(7312..7953)
                     /locus_tag="PKX82_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1109 domain-containing protein"
                     /protein_id="HOF51095.1"
                     /translation="MKTDELISLLATSAAPVPAHAVGRRFAMALGMGIVGAALLMLMT
                     LGLRPDLLQVATEPSFWMKLAFAACLALAGLVATERLSRPGVRVGFAWAAMAAPVLAL
                     WLAAAIALLGAVQEERLDLILGNTWKSCPFNIAMLSVPLFIATFWCMKDLAPTRLALA
                     GASSGLLSGALSALVYALHCPESAAPFLGVWYVLGIAIPTLAGALLGPRILRW"
     gene            complement(7950..8525)
                     /locus_tag="PKX82_02245"
     CDS             complement(7950..8525)
                     /locus_tag="PKX82_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOF51096.1"
                     /translation="MERTHPRDARFLETESRLRELLVLGLGGDSAAYHGFLKALGAHL
                     RAYFRKRLFQRPDEVEDLVQETLLAVHNQRHTYRSDQPLTAWVHAIARYKLVDMLRAR
                     ASREALTDPLDDELEVFAQSDTDAADAKRDLGKLLDTLPDKQRLPIVHVKVEGLSVVE
                     ASKLTGMSESAIKIGVHRGMKALAARIRGMV"
     gene            8696..8974
                     /locus_tag="PKX82_02250"
     CDS             8696..8974
                     /locus_tag="PKX82_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HOF51097.1"
                     /translation="MITRSLTATTLALAALSSGAAMAQEKPMDGKPAMEKCFGVAMAG
                     KNDCAAGPGTTCAGTSKMDYQGNAWKFVPAGTCTTIKTPKGMGSLKSM"
     gene            9002..9844
                     /locus_tag="PKX82_02255"
     CDS             9002..9844
                     /locus_tag="PKX82_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HOF51098.1"
                     /translation="MRYPDITAGLGLKLQHFDAALENADTGMWFEVHPENYLVDGGPR
                     RALLECIRARHPVSLHGVSLSLAADVAPDQAHLARLAALVEQIEPVLVSEHLAWSAWR
                     GMYLPDLLPFPRTQAALSRIASNIACTQDALGRRIAIENPSHYIAIAGHDWDEIGFLT
                     ELTRRTGCGLLLDVNNVYVSANNQGYSAEAYIDTFPADALMEIHLAGHSLDPQLGKGL
                     LIDSHDAPIAPEVWRLYERLIARCGPCPTLIERDEQIPAFADLLAERGKAHALLCNTE
                     LACV"
     gene            9841..>10294
                     /locus_tag="PKX82_02260"
     CDS             9841..>10294
                     /locus_tag="PKX82_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOF51099.1"
                     /translation="MNGTLARFQDDFVQSLLAPQSDMPSTLRTLTLQPGFAVYRNTVM
                     KGCIDALQANFPAVLKLVGEEWFRAAAALHVRAQPPDDARLLHYGVHFPAFLRNFAPA
                     TELPYLWAVAQLDRFWTEAHAASDADVLTPSVLADLLPAQMGELVLNPH"
BASE COUNT         1910 a         3158 c         3307 g         1919 t
ORIGIN      
        1 tcactttcca cgcctggtat cccggctgcc cgatggaaga agacgcctac ggcatcccca
       61 agcccaagga caccgaggtc atcgtgccca cgctgctgtt tgcaccctgt gtgggctatg
      121 gtcctggtgg ctatcgcctg ggttacggcg gcggttttta cgaccgtatg ctgtcctcgc
      181 tggaacccag acccttcacg gtgggcttgg cttttggcac cggctttgta gaagatttcg
      241 aacccgaacc ccacgacatg ccgctggacg ccatcctcaa cgaccacggt gtggtctggc
      301 ccatcaactg accatgtcgg ttacccagga cctgctggac ccgctgctcg acgctacctt
      361 caaggggttt ccgcaccaca gcgggccgct gcgccgttcg cagatagggg cccaaggctg
      421 gaacgtgctg cgtggcgatc tgccgctgcc gattgccctc atcaagcagg acctgctgcg
      481 ccacaacctg cgctggatgc aggactttgc cgcgtcccgc gggctggggc tggcaccgca
      541 cggcaagacg tccatgtcgc cgcaactgtt ccagatgcag atggatgcgg gcgcctgggg
      601 catgactttt gccacggtga cacagatgcg caccggcatc agcgccggcg tgcagcgctg
      661 cctgattgcc aaccaagttt ttactgcgct ggatttgcag ggcattgatg ccatgcggcg
      721 cgaacatccg ggactgcgcg tggtgtttct ggtggattct gcgcagcagc tggccctgat
      781 caaggcctgg cgccagaaga acccgtcggc gcaacccttt gaggtgctgc tcgaaatggg
      841 ggtggatggc gggcgcacgg gttgccgtac tgcagagcag gccctggaac tggccgggcg
      901 gctgcacaac agttcggccg tgcggctggt gggtgtggag tgctacgaag gtctggcggc
      961 gcagggaaac acggcgcagg accagcttta cgcccagagc ctgatggacc gggtggaggc
     1021 ggtggcgcgc gcctgcgacg cccagaatct gtttgacagc gacgaggtca tcgtcaccgc
     1081 cggcggttcg gccatttttg acctggtcgc cgcacggctc aaacccgcgc tgcgtcgccc
     1141 ggtactgggt ctgctgcgct cgggctgcta cgtgacgcac gaccagggca cctaccaacg
     1201 catgatgcgc gcggtggccg aacgcaccgg ctgcaccgac gggctggaag ccgccatgga
     1261 agtgtgggcc tgcgtgcagt cctgccccga gccggggctg gtgattctgg cggcgggcaa
     1321 acgcgacctg tcctacgact gggagatgcc acgcccgctg gcggtgctgc cccaatccgc
     1381 cgtggaacgg gaccccgccc atctgcagcc ggtaggggag gactggaagg tggtggcgct
     1441 caatgaccag cacgcctatc tgcgtggcag cggcgcggcc ttcgaagcgc tgggcgtggg
     1501 cgacctggtt ggcctgggta tctcgcaccc ctgcaccacc tttgacaaat ggcgctggat
     1561 gccggtggtc gatgcgtcct acaccgtatg cgacgccatc accacctgtt tctgaaacgc
     1621 tagcgtgccg cgctcagtgc acgggcatcg ttctcgtatc cctgcaaaac ctgcgagagt
     1681 ttggtgcgct gggccggcgt tgcgctgttg tgcagcacag ccagcagatt gcagctgtgt
     1741 tgccgcatgc ggtcctggta ctggcgcagt gcagcttgcg gggaatgcac actgcggttg
     1801 agcaacgcct gcagttccgc ctgggcctgt gcttcggtgg cgttgcccga gcgcaatgcc
     1861 tggagggtct gcagggtgtc ctgctggcgg cgcagccttt cccggtacag ggtgttggct
     1921 tccagcggcg acgcctccag cagtgcgcgc atgcgctctg cttgcccatc gctcaaacgc
     1981 ccatagaacg actcggcgcg ttccacaaat tttttcaggc ggtcacttgc gcgctcagcg
     2041 ggggtgccgt ccagccagtc ttcgcgccac tcctggttgc gtttttccca ggtgcgttcc
     2101 aggtgcacca gttgggatgg ctgcaacaac ggtgccatcg ctgcggccgt gggcaggatg
     2161 cgtccggtcg cgatcagcag ccgcgtctcg atggtggtgt aaatgctgca gacctgctcg
     2221 gccgacacgg tctggttgac caggggctgc aggcctttga gcaattctgc gtactggggc
     2281 agttcttcct tgcggtgcca gtcctgccag gtctgcaggt cggcccgcac ccgcagggtt
     2341 tggggcgtgt cgaagtccag gtagctgtcg agccaccagt aggtcaggtc gggcgcattg
     2401 ttgtagccca gacggaccgc gctacagccg cccatgatca tcgcgctggc gagcacgctg
     2461 ataatggcga accagcgaat acaggaacgc aagaaaaggg acatgaacgt ggtttcaaca
     2521 caaaggaatg gggaagaggg catccaccgc aatccggtgg acgtggtcat tatggcggcg
     2581 ggcaagggaa cgcgcatgaa gagcaagctg cccaaggtgt tgcaccagct cgccgggcgc
     2641 cccctgttgc agcatgtggt ggacacggcc ggcgaactga tggcgcgcaa ggtggtggtg
     2701 attaccggcc acggtgctat cgaagttgaa gcagctctcg cagtcaacac gggggctaga
     2761 gccgaatttg ccttgcaatg tgtgcgccag gatccgcagc tgggtaccgg ccatgccgtg
     2821 cagcaggcgg tgcccgcgct ggacgacgac ggcatcgtgg tggtgctctc gggggatgtg
     2881 ccgctgacgc aatccacgac gctaatgcac ctgatcgctg cgtgcgctgg caagagcctg
     2941 gccctgctga cgctcgaaat gcccaatccc gcgggctatg gccgcatcgt gcgtgccggt
     3001 ggtacgcgcg atggtgccgt gcaggccatc gtggagcaca aggatgccag cgatgcccag
     3061 cgccagatca ccgagatcta cagcggcatc atggccgtgc ccgcggcgca gctcaagcgc
     3121 tggctggccc ggctggacaa caagaacgcc caaagcgagt actacctgac cgacatcgtg
     3181 aagtttgcgg tggccgacgg ggtgccggtg gtgggccaca agattgccga tgcggtgcag
     3241 gtggctggtg tcaacagccc ggtacagctg gccgaactgg agcgcgccta ccagctgcgc
     3301 caggcccacc gcctgatgga agaaggcgtg cgtcttgcgg acccggcacg catcgatgta
     3361 cggggcgatc tgctgtgcgc gcacgatgtg agcatcgacg tgaactgcgt cttcatgggg
     3421 caggtcagcc tgggcgaagg cgtgtccatc ggcgccaact gcgtgattgc caatgcagtc
     3481 atagaggcag gagcggtgat ccatccgttc acccacatcg acggagaaaa gatgggcgtg
     3541 accgtggggc cgggcgcgct gattggtccg tttgcgcgcc tgcgccccgg tgcgcggctg
     3601 ggggccgaag tgcacattgg caactttgtg gaagtgaaaa actccacgct ggccgcaggt
     3661 gccaaggcca accacctggc ctatctgggc gatgccacgg tgggcgagcg cgtcaactac
     3721 ggcgcgggca gcatcaccgc caactacgac ggtgccaaca agcaccgcac cgtgatcgag
     3781 gctgacgtgc atgtgggcag caactgcgtg ctggtggcgc cggtcacgat tggggccggt
     3841 ggaacggtgg gcggtggctc caccatcacc aagagcactg agcccggcgc gttgagcgtg
     3901 gcgcgcggcc ggcaaatcag cattgcccac tgggagcgcc ccaggaaggc acccaagtcc
     3961 tgacaggaga ccgccatgga tgacgcttct gcccaacgta tcgcacaact ggaggccgac
     4021 ctggccgctg cccggcgcga gatgcaggac ttcacctaca ccgtgtcgca cgacctgcgt
     4081 gcgccgctgc gccacatcat ttcctatgcc cggctggcgc aggaagatgc agggccgcaa
     4141 ctgagcggtg atgtgctggg ctttctggcc acggtgacgg attcggcgcg caacatgggc
     4201 gccatgctcg atgccctgct ggagctgtca cgcttgggga cggtggcgct ggatgtggag
     4261 tcggtacctt tgcaggcgat ggtggaggac gtggccttgt ccttgcgcaa cgcccatccc
     4321 cagcgtgcga tcgactggcg tattgaactg gatgcgtgtt ctgtgaccgg cgacgccagc
     4381 ctgctgcgcc aggcattggt gtgcgtgctg gacaacgcga tgaagttcac cgcgccgcgt
     4441 gcacacgccg aagttgccgt ctcagcccat gccgacgcgc cagcgcagcg cgtggtgatt
     4501 gcggtgcagg acaacggtgt cggtttcaac ccggtgttgc aagacaagct gtttcagcct
     4561 tttcaccggt tgcacagcag cagccagttc agtggcctgg gtctggggtt ggcccaggta
     4621 cgcaagatcg tggtgcgcca tggcgggcag gtggccatcg cggcccgcgt ggacggtggc
     4681 tgtacggtca cattgacctt gccgctggcc tagctcagcg gcagctcggc cccaaacttg
     4741 ctgcgtttga cgctgaagaa cgccttgacg ttgcgcacgt tggcgtccac cgtgaacagc
     4801 cgctgcgtca acgcctggta ggccggcatg tcgcgggtgt gcactaccag gcagaagtcc
     4861 ggcccgggag atacgcggta gcactgctgt accgccgcgt cctgggcgat gcgggtttca
     4921 aaggcttcca gtgcgcccgc atcctggcgg tccagcgtga tctccacgat ggcggtcaat
     4981 ccatggccca gcgtctgcgc catgcgctcc gggttgagca ccgccacctg gcgttcgatg
     5041 aggcccgcct catgcagccg tttgactcgc cgcaggcagg tgggcggcga ggtgtgcaca
     5101 cgctcggcca gcgcctggtt gctgagggat gcatcgcgct gcaactggtc cagcagcagc
     5161 aggtcgattg catcgagttc aattgattcc ataaacgtca gaaagttggt attaaattac
     5221 attcaatggt ctgcatgaaa ttttcagtca attcagcgta aatatcaatc agatattttt
     5281 ctgcatccgt cctaggatgc atccaattcg attcattggg agaaaagcta tgtgtggcat
     5341 cgtcggcgcg gtatctaccc gcaacatcgt tcccgtgttg gttcagggcc tggagcggct
     5401 cgaataccgt ggctatgact cctgcggcgt ggccgtctat gcgcaggatg gcacacccgg
     5461 cctgcgccgc gcgcgcagca cctcgcgcgt ggcggaattg atggagcagg tggcagccgg
     5521 gtcggtgcag ggcacgctgg gcattgccca cacccgctgg gccacccatg gcgcacccgc
     5581 ggtccataat gcccaccccc atttcagcca tggccccggc gccgatgttt cgggcaagcc
     5641 gggtcgtata gcgctggtgc acaacggcat cattgaaaac cacgacgagt tgcgcgcagc
     5701 cctccaggcc aaaggctatg tattcgacag ccagaccgat accgaggtga ttgcacacct
     5761 gatcgacagc ctgtacgacg gcgacttgtt cgaggctgtg aaggctgcca ccgtgcaact
     5821 gcacggcgcc tacgccatcg cggtgttctg caaggacgag ccgcaccgcc tggtgggtgc
     5881 gcgtgcgggt tccccgctga tcctgggtgt gggcaaagac gggcaggagc atttcctggc
     5941 cagtgacgcc atggccctgg ccggtgtgac ggaccagatc gtgtacctcg aagagggcga
     6001 tctggtggat gtgcagctgg gcaaatactg gctgttcgac aaggcgcaca aagccgtggc
     6061 acctgcacag cgtccggtga agacggtgca tgcccacagc ggtgccgccg aactcggccc
     6121 ttatcgtcac tacatgcaga aggaaatttt cgagcagcca cgcgccattg ccgacacgct
     6181 cgaaggcgtg gagggcatcg cccccgaact gtttgatggg cagatgaacc atgcccccgg
     6241 cgccaacgca gcgcgggtat ttgcccagat cgactcggtg ctgattctgg cctgtggcac
     6301 cagctactac agcggctgta cggccaagta ctggatcgag agcatcgcca aagtgccctg
     6361 tcaggtcgaa gtggccagcg aataccgcta ccgcgactcg gtacccaacc ccaacacgct
     6421 ggtggttacc atcacccaga gcggagaaac cgcagatacg ctggccgccc tgcgccacgc
     6481 ccagtccttg ggtatgaagc acacgctgac catttgcaat gtggccacca gtgccatggt
     6541 gcgcgaatgc agtctggcct atgtaacgcg cgcgggggta gaaatcggtg tggcgtctac
     6601 caaggcgttc acgacccagt tggcgggcct gttcctgttg accctgtgcc tggcgcaggc
     6661 caagagccga ctcagtgacg cagaagaagc gcgccatctc aaggccatgc gccacctgcc
     6721 cactgcgctg caggccgtac tggcgctgga gccgcaggtc atggcgtggg cggaagactt
     6781 tgccaggaag gaaaatgccc tgttcctcgg ccggggtttg cactacccga ttgcgctgga
     6841 aggcgcactc aagctcaagg aaatcagcta catccacgcg gaagcctacc ccgctggcga
     6901 actcaaacat ggcccgctgg ccctggtgac cagcgagatg ccggtggtga ccgtggcgcc
     6961 taacgacgcg ttgctggaaa aactcaagag taatctgcac gaagtgcgcg cccgaggagg
     7021 cgtgctgtat gtgctggccg atggcgacac ccgcatcgaa agcggtgaag gtctgcacgt
     7081 gatccgcatg cccgagcact acggtgagtt gtcacccctg ttgcacgtgg tgccgctgca
     7141 aatgctggcc taccacacgg cctgcgcccg cggaaccgat gtggacaagc cgcgcaacct
     7201 ggccaagagt gtgacagtgg agtaagggcg ctgcgatagc cctcggtcaa cgtccggtaa
     7261 gtgggaacag aaacttaccc aagagcacga aatcagaagt gtctggtaaa actaccatcg
     7321 caaaatgcgc ggccccagga gcgcgccggc cagtgtcgga atggcgatcc ccagtacata
     7381 ccagacgccc agaaaagggg ccgccgattc cgggcagtgc agtgcataga ccagcgcgct
     7441 cagtgcaccc gacagcaagc cagagctggc gcccgccagc gccagtcgtg tgggtgcaag
     7501 atctttcatg caccagaacg tcgcgataaa cagcggtacc gagagcatgg caatgttgaa
     7561 cgggcaggat ttccatgtgt tgcccagaat caggtcaaga cgctcctcct gcacagcccc
     7621 gagaagtgca atggctgctg ccagccaaag ggccagcacc ggtgctgcca tcgccgccca
     7681 ggcaaatccc acacgaacac ccgggcggga cagtcgctcg gtggccacca gtccagcaag
     7741 tgccaggcac gctgcgaaag ccagtttcat ccagaaactg ggctcggtgg cgacctgcag
     7801 caggtcgggt cgcaagccca gtgtcatcag catcaacagg gcggctccca ctatccccat
     7861 gcccagtgcc atggcgaagc ggcgacccac cgcgtgggca ggtactggcg ctgcactggt
     7921 ggctaatagc gaaataagtt catccgtttt cataccattc ccctgattct ggcggccaat
     7981 gccttcatgc cccggtgcac accgattttg atggcggact ccgacatgcc cgtgagtttt
     8041 gacgcctcga caaccgacaa gccttccact ttcacgtgca cgatgggcaa acgctgcttg
     8101 tcaggcagag tgtccagcaa tttgcccaga tcccgctttg cgtcggcagc gtcggtgtcg
     8161 gactgggcaa acacttccag ttcatcatcg agcggatcgg tcagcgcttc acgcgaagcg
     8221 cgggcacgca gcatgtccac cagcttgtag cgcgcaatcg cgtgcaccca tgccgtcagc
     8281 ggctggtcgc tgcggtaggt gtggcgctgg ttgtgcaccg caagcagtgt ttcttgcacg
     8341 aggtcctcta cttcgtccgg gcgctggaac agccgctttc tgaaataggc acgcaagtgc
     8401 gcacccaggg ctttcagaaa gccgtggtaa gccgctgagt cgccgccgag cccaagaacc
     8461 agcagctcgc gcagccggct ctcggtctcc aggaaccggg cgtctcttgg gtgagttcgt
     8521 tccattgcgg gtttgggttg cagaaggtgc aaaaaatatt tttgaagaat tttgcggagc
     8581 actgtaaccc gcagccgtgt tgcaccgaac taatgctcag actgtaacgg acgcctgctc
     8641 aagggccagc acagtctgtc tcaatccacc catgtacttc acaggagtta ttttcatgat
     8701 cacacgttct ctcaccgcta ccactctggc tcttgcagcg ctgtccagcg gtgctgccat
     8761 ggcccaggaa aaacccatgg acggcaaacc ggccatggaa aagtgctttg gcgtcgccat
     8821 ggccggcaag aacgactgcg ctgcaggccc aggtaccacc tgtgcaggca cgtccaagat
     8881 ggactaccag ggcaatgcct ggaagtttgt gcccgctggc acctgcacca ctatcaagac
     8941 tcccaagggc atgggctccc tgaagtcgat gtaagtcgtt gaacgcagct agggcgccat
     9001 catgcgttat cccgacatca cggctggcct cggactgaaa ctgcagcatt tcgatgctgc
     9061 gttagaaaat gccgacaccg gaatgtggtt cgaagtgcac ccggaaaatt acctggtgga
     9121 tggcggccct cgccgcgcat tgctggaatg catccgggct cgccacccag tatcgttgca
     9181 tggcgtttcg ctgtcgctgg ccgcagacgt tgcgccggac caggcccatc tggcacggct
     9241 ggccgcattg gtcgagcaga ttgaaccggt gctggtttca gagcatctgg cctggtcggc
     9301 atggcgtggc atgtatttgc cagaccttct gcctttcccc cgaacgcagg cggcgctctc
     9361 gcgcattgcc agcaatattg cctgtaccca ggatgcattg gggcggcgta tcgcgattga
     9421 aaacccctcg cactacatcg ccatcgcggg gcacgactgg gatgagattg gcttcctgac
     9481 agagttgacg cggcgcaccg ggtgcggact gctgctcgat gtcaacaacg tgtacgtcag
     9541 tgccaacaac cagggttatt ccgccgaggc gtatatcgat acatttcccg ccgacgcgct
     9601 gatggaaatc catctggcgg gccacagcct cgatccgcaa ctcggcaagg gcctgctgat
     9661 cgactcgcac gatgcgccca ttgcacccga agtctggcgt ctctatgaac gcttgattgc
     9721 gcgctgcggt ccgtgcccca ccctgatcga acgcgacgaa cagatcccag cgttcgccga
     9781 cttgctggcg gaacgcggca aggcgcacgc cttgctgtgc aacacggaac tggcttgcgt
     9841 atgaacggca cacttgcccg cttccaggat gactttgttc agtccctgct ggcaccgcag
     9901 agcgacatgc cgtcgaccct gcgcacgctg acgcttcagc cgggcttcgc ggtgtaccgc
     9961 aacacggtga tgaagggatg tattgatgcg ctgcaggcca atttcccggc ggtgctgaag
    10021 ctggtgggag aggagtggtt tcgcgctgcg gcggcgctgc atgtgcgtgc gcaaccgcca
    10081 gacgatgcac gcctgctgca ttacggggtg cactttcctg cgtttctgcg gaacttcgcg
    10141 ccagcgacgg agttgcctta cctgtgggct gtggcccagc tcgaccgatt ctggacggag
    10201 gcccatgctg ccagtgatgc ggatgtattg acgccgtccg tgcttgcgga cttgcttcct
    10261 gcgcaaatgg gtgagcttgt attgaaccca catc
//
LOCUS       DAOSJM010000035        10194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6404_length_10194_cov_4.395108, whole genome shotgun sequence.
ACCESSION   DAOSJM010000035 DAOSJM010000000
VERSION     DAOSJM010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6404_length_10194_cov_4.395108"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..2280)
                     /gene="glgX"
                     /locus_tag="PKX82_02265"
     CDS             complement(106..2280)
                     /gene="glgX"
                     /locus_tag="PKX82_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOF51100.1"
                     /translation="MNTMHALAPWTECDTGRPWPMGVVPCTVDGQTGLNVAVYARHAT
                     AVEVCLYDETGEEETARVRLPACTGGIWHGFIPGPTPGQRYGLRAHGPWDPASGHRFN
                     PARLLIDPWAHSLSGPLSALALEIGHQLDAPDLPNPQDNADRIPKARVVDMTAELRAG
                     AAITAGPQTPMARTILYEAHAKALTALHPEVPPLQRGTYAGLASPAMLKHYQHLGLTT
                     LCLLPVQLHVDERHLLELGLTNHWGYNTLAFFVPDPRYATDAARGTGDDDAGVRTEFR
                     QMVDTLHRNGIEVILDVVYNHTAEGDASGPTLSWRGLDNASWYALDAQAQHVNPSGCG
                     NTLNMGEDRVIQLIMDSLRWWVQAYGVDGFRFDLAVALGRDPAWGNRFQPGGALFAAL
                     AQDPVLAGVKLIAEPWDIGHDGYQAGRFGPRWHEWNDQFRDTVRAWWLGHACTRGQIA
                     RRLAGSNDLFEASGRSPLTGVNLITAHDGFTLADLTSYQHKHNEANGEENRDGHGHNL
                     SANAGHEGPSNEPAILTLREQWRRALLATLFCAQGTPQLLAGDELGHSQRGNNNAYCQ
                     DNPITWLDWSAADAQLTSFVAGLVRLRQRYAGLRHPTWFHGKLLGGSSKPDMEWRTAD
                     GTPPDIAAWELPDGRLLTYVITVGEPGKPPLERLMLILLAGERSIEVSLPDGEWDLLM
                     DSARGWVGDNTPPTTLASSLLVEPPTMLVLVQPLQTHPVTTP"
     gene            complement(2277..4475)
                     /gene="glgB"
                     /locus_tag="PKX82_02270"
     CDS             complement(2277..4475)
                     /gene="glgB"
                     /locus_tag="PKX82_02270"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOF51101.1"
                     /translation="MPFSHQEHALTQQLQQGRCRDPFSHLGPHRKSDSDGITLRVYLP
                     GARGVSAVDRGTGEVLAALLASGDGGLFVGHLPPGSAGDYRLAVDYPGHHLVQEDPYR
                     FAPWLGDVDVWLLAEGKHLRPWQKLGAHPVTLEGVQGTVFAVWAPHAQRVSVVGDFNG
                     WDGRLHPMRWRVECGVWEIFLPGVRTGALYKFEVLDAAGQCTLKADPYARRSEGPPGN
                     AAWVCDSPVAAQPVDTAEPEGTHHESIAAPMAIYEVHVGSWRRPDGQMPDWDYLADTL
                     VPYVVDMGFTHIELLPISEHPFYGSWGYQPTGLYAPTARYGTPEALHRFVGAVHAAGL
                     KVILDWVPGHFPSDAHALARFDGTALYEHADPREGRHRDWETLIYNFGRNEVRNFLVG
                     NALFWIEQYGVDGLRVDAVASMLYRDYSRPAGEWIPNRDGGRENYEAIAFLRETNDIL
                     HREAPGALTLAEESTAFPQVTGATAQNGLGFDYKWNMGWMNDTLAYFALDPMYRRWHH
                     DRITFAMMYAYSEHFVLPLSHDEVVHGKGSLLGKMAGDPWQKRANLRALYALMYAFPG
                     KKLMFMGCELATPTEWNHDSELDWSLLNHPDHAGVQRLVRDLNHAYRAHPCLHSRDSD
                     PGGFQWISADDCDNSVFSFMRLGHKDHVPLAVVCNLTPVPRDGYRIGVAHGGAWRECL
                     NTDSAHYGGTNAGNQGRVQAEPIPIHGYPRSLSLHLPPLSVIWLEAKGVA"
     gene            complement(4518..6983)
                     /locus_tag="PKX82_02275"
     CDS             complement(4518..6983)
                     /locus_tag="PKX82_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51102.1"
                     /translation="MTALILPASDLRRTVDPASLGFSDTSELLHHPLPWIGQERAEQA
                     ARFGLTMDQPGYNLFVLGEVGSGRSTLMGQMMRNEAAKRPMPPDLCYLHNFDEPEHPR
                     ALRLPAGEGRLLRQLMGQFAKNLQSDIPKRLSEPDFKAESERLANINKAEEERAYVEL
                     SAFADAHNFVLMREQGNMVFTLRDTKGEPLTAGKAMALTREERAEIDGAEATLRNEIS
                     RFLDKSRAMEQALNEAMAALRRQTVKPMLDHAMQLIRNGLRKQIKDTVKLGSYLDQVH
                     HEVMENIEVFQPGEDEEIRLQALIEVVSHFRVNVAVDNHGLEGAPVILEDNPLFRRLF
                     GSIEYEADDDMLVTDFSRIRAGSLVKAHGGYLMLHLRDLLADEPVWEKLRRFLRSGRL
                     QIEEPGMMYAPIAAVSLQPEPIDVDVKIVLIASVDDYYAVQEGDPEFARRFRCKVDFA
                     ESFKATPESCSATAIFVAHICRHLGLAHFTAAAVAVLIEVTHREAEDQMRQSAIFAHS
                     EALVMESSAMARSRGANLVEAQDVRAAMQARMHRHSYPEERLQDTIVDGERLLEVRGE
                     RVAQLNGLTVVDLGDYQFGFPVRVTGRTYAGEEGLLNIEREVKLSGPIHDKGVLILHS
                     YLSALFAHIAPLALNAAVVFEQEYSGVEGDSASCAEFYALLSSLSGLPIRQGIAVTGA
                     VNQHGEMLPVGGINEKIEGYFRSCELLGLDGQQGVLIPARNRRHLMLDPRVVEAVAQG
                     RFHIYGAELASEGMELLTGVPFGALVGADYPPDTVLGRAQRTLQAYRYACETPRPVTG
                     ARRLLKSHLEPKPSTGNRTEGHW"
     gene            complement(7052..8983)
                     /locus_tag="PKX82_02280"
     CDS             complement(7052..8983)
                     /locus_tag="PKX82_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HOF51103.1"
                     /translation="MRLLRQIAFIARAEGRFFVRFPKLLLATLAVAVMPSVYAVIYLS
                     SVWDPAAHAGALPVALVNLDEGVEYREHVFNAGWDVAASLRKKKTFGFQDVADPQEAR
                     RLVREGAVAFALIIPKDFSSNAIPGWEAGGGRLVVFTSEGNNFEAARIARVFAQELGH
                     EVNATLNERRWRLVLSNAAGSQRSVDRLRAGVDELRSGAHELSGGAKQAAKGAYAAAT
                     GSDRLYDGVTQLTGGVKQLASGLRTMDARHPSASELTRLKTGADELAAAHVELGKGLA
                     ELKSGSGQLREGVQTFRSEAKSSLLVPSSVVDGLDQVLDGTTQLDSGLLASVNAQHRM
                     AAGASQLSAGVDTLTSGMLAMNSGVRTMVVKLPQDRQLDELDTGASAVASGTAVLADG
                     TQKIKAGVDRLTTGLDLLADSLPSSVDGPDGSAQGLANSVKPVLEVEAPVSNSGSGFA
                     PNVLPAALWLGAGIAAFLIHVRVLPRHAQFFSRPAQTLGKLAIPAAIVLSQATILLAT
                     VMLILKIPIVHPVAFSLTLGATALTFLGIVFALTRAFGDAGKALAMIFLAVQLSSSGG
                     VLPVELSGGVFANISPWLPLTWVVRAVKASMFGAYGGAWGHPLLIVTLGGVLSLLCAS
                     YVGRWRFVRASQVRPAVDF"
     gene            9036..10124
                     /gene="selD"
                     /locus_tag="PKX82_02285"
     CDS             9036..10124
                     /gene="selD"
                     /locus_tag="PKX82_02285"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOF51104.1"
                     /translation="MNSTAPQSPTAPAEPRLTSLSHGGGCGCKIAPGVLSDILKTTTA
                     MPLPPQLLVGIETADDAAVYQLNDEQALIATTDFFMPIVDDAFDFGRIAATNAISDVY
                     AMGGTPIMALALVGMPINVLSTETIGQILAGGAAVCKAAGIPIAGGHTIDSVEPIYGL
                     VALGLVHPKRVKRNADARVGDRLILGKPLGVGILSAALKKEKLGAEGYARMIAVTTQL
                     NTPGPDLAALDGVHAITDVTGFGLAGHGLEVARGANCTVHIDWARVPLIEGVADLAAQ
                     GMVTGASGRNWAAYGSQVALAPELSPVDRALLSDPQTSGGLLVSCAPEAVAQVLEIFS
                     RHGFAHATEIGSVSVADADPAGPRMRVR"
BASE COUNT         1771 a         3275 c         3295 g         1853 t
ORIGIN      
        1 ccaggtctcc cgatccatgg cgacctggca gactggtgat gtgcagtagc acaccgctgg
       61 cgcgacgtgc cagcaagggg tgaatcgggg cgatggcagg atccctcatg gagtggttac
      121 ggggtgggtt tgcaggggct ggaccaagac cagcatcgtt ggcggttcca ccagcaagga
      181 cgatgccaat gtcgttggcg gggtgttgtc gccgacccag ccacgggcac tgtccatcaa
      241 cagatcccac tcgccgtcgg gcagactgac ctcgattgac cgttctccgg ccagcaggat
      301 cagcatgagc cgttccaggg gcggcttgcc cggctcgccc acggtgatga cgtaggtcag
      361 caggcgccca tcgggtagtt cccacgccgc aatatcgggg ggggtaccgt cggcggtgcg
      421 ccattccata tccggtttac tgcttccacc caggagcttg ccgtgaaacc aggtggggtg
      481 gcgcagaccg gcatatcgtt ggcgcaaccg caccagcccc gcgacaaagg atgtgagctg
      541 ggcgtctgct gcactccagt ccagccaggt gatgggattg tcctggcagt acgcgttgtt
      601 gtttcctcgc tggctgtggc ctaactcgtc tccggccagc agttgcggcg tcccctgggc
      661 gcagaacagg gtggccagca gtgcccgacg ccactgctcg cgcagggtca gtatggcggg
      721 ttcattgctc ggaccctcat gccctgcgtt ggcgctcagg ttgtggccgt ggccgtcgcg
      781 gttttcttcg ccgttggctt cgttgtgctt gtgctggtag ctggtcaggt ccgccagggt
      841 gaacccatcg tgggcagtga tcagattgac tccggtcaat gggctgcgcc cagaggcttc
      901 gaacaggtca ttggacccgg ccagcctgcg tgcgatctgt ccgcgcgtgc acgcatgacc
      961 caaccaccag gcacggaccg tgtcgcggaa ctggtcattc cactcgtgcc agcgcgggcc
     1021 gaaccgacct gcctggtagc cgtcgtgccc gatgtcccag ggctcggcga tcagcttgac
     1081 gccggccagc accgggtcct gtgccagtgc agcgaacagt gcgccgccgg gttggaatcg
     1141 gttgccccat gccgggtccc ggcccagtgc cacggcgagg tcaaagcgaa aaccgtccac
     1201 gccataggct tgtacccacc agcgcaggct gtccatgatg agttgaatca cacggtcttc
     1261 acccatgttg agcgtattgc cgcagccact ggggttgacg tgctgcgcct gcgcatcgag
     1321 tgcataccag ctcgcgttgt ccagtccacg ccacgacagg gtgggtccag aggcatcacc
     1381 ttcggcggtg tggttgtaga caacatccag gatgacttcg atgccgttcc gatgcagggt
     1441 gtccaccatc tggcggaact ccgtacgcac gccggcgtca tcgtcgcccg tgccgcgcgc
     1501 agcatcggtg gcgtaccgcg ggtcaggcac aaagaatgcc agggtgttgt aaccccaatg
     1561 gttggtcagg cccagttcaa gcagatgccg ctcgtccaca tgcagttgca cgggcaacag
     1621 acacagtgtg gtcagcccca ggtgctggta gtgcttgagc atggcagggc ttgccaaccc
     1681 cgcgtaggtg ccacgttgca aagggggaac ctccgggtgc aaggccgtga gggccttggc
     1741 gtgggcctca tacagaatcg tgcgcgccat gggggtttgg ggtcccgccg tgatggcagc
     1801 gccagcacgc agttccgcgg tcatgtcgac gacccgtgcc ttggggatgc ggtcggcatt
     1861 gtcttgaggg ttgggcagat ccggtgcgtc cagctgatgc ccgatttcga gcgccagtgc
     1921 ggacaaaggt cccgacaggg agtgggccca gggatcgatc agcaggcgtg caggattgaa
     1981 gcgatgtccg gaagccggat cccaggggcc gtgcgcgcgc agaccataac gctgtccggg
     2041 cgtcgggcca ggaataaacc cgtgccaaat gccccccgtg cacgcaggta accggacacg
     2101 ggccgtttcc tcttctcccg tttcatcgta gaggcaaact tccacagcgg tggcatggcg
     2161 tgcgtagacg gccacgttca ggccagtctg gccgtccacg gtgcagggaa ccacacccat
     2221 gggccatggg cgcccggtgt cacattcggt ccatggcgca agcgcatgca tggtgttcat
     2281 gccacgcctt ttgcttcaag ccagatcacg ctcagcgggg gcaggtgcag gctcagtgat
     2341 cttgggtacc cgtgtatcgg gatgggctca gcctgtacgc ggccctggtt gcccgcgttg
     2401 gtgccgccgt agtgggcaga atctgtgttc agacattccc gccaggcacc tccgtgtgcc
     2461 acgccaatac ggtagccatc gcgtggaacc ggggtcaggt tgcaaacgac ggccaatggc
     2521 acgtgatcct tgtggcccag acgcataaag ctgaagaccg agttgtcaca gtcgtcggcg
     2581 ctgatccact ggaagccacc gggatcgctg tcccggctat gcaggcacgg gtgggcgcgg
     2641 tacgcgtggt tgaggtcccg gaccaggcgc tgcacgccag cgtggtcggg gtggttcagc
     2701 aggctccagt ccagttcgct gtcatggttc cattcggtgg gggtcgccag ctcgcacccc
     2761 atgaacatca gtttcttgcc gggaaaggcg tacatcaggg cgtacagcgc gcgcaggttg
     2821 gcgcgcttct gccatgggtc gcccgccatc ttgcccagca gagatccctt gccatgaacc
     2881 acttcgtcat gcgagagcgg aagcacgaaa tgctcgctgt aggcgtacat catggcaaag
     2941 gtgatgcggt catggtgcca gcggcggtac atcgggtcga gcgcgaagta ggccagtgtg
     3001 tcgttcatcc agcccatgtt ccacttgtag tcaaagccca atccgttttg tgcggtggcg
     3061 cctgtgacct gtgggaaggc cgtggactcc tcagccaggg tcagggcgcc gggggcctcg
     3121 cggtgcagga tgtcattggt ctcgcgcagg aaggcgatag cctcgtagtt ctcgcgtcca
     3181 ccgtcccggt tgggaatcca ctcgccggct ggccggctgt aatcccggta gagcatggac
     3241 gcgaccgcgt cgacccgcag accatccacg ccgtactgct caatccagaa cagtgcattg
     3301 cccaccagaa agttgcgaac ttcattgcgg ccgaagttgt agatcagcgt ttcccagtca
     3361 cggtgccgtc cttcccgtgg gtcggcatgt tcgtacagcg cggtaccgtc gaagcgggcc
     3421 agtgcatggg cgtcggatgg gaagtggccg gggacccagt ccagaatgac tttcaaaccc
     3481 gcagcgtgga cagcaccgac aaaacggtgc agcgcttccg gggttccata gcgtgccgtg
     3541 ggtgcataca gacccgtggg ctggtagccc caggaaccat aaaaagggtg ctcactgatg
     3601 gggagcagtt cgatgtgggt gaaacccatg tccaccacgt acggcaccag ggtgtccgcc
     3661 agatagtccc agtccggcat ctggccatcc gggcgcctcc aggagcccac gtgtacttcg
     3721 taaatcgcca tgggcgctgc gatgctttcg tggtgggtgc cctctggttc cgcggtgtcc
     3781 accggttgtg ctgctactgg cgaatcgcac acccacgctg cgttgccagg tggcccttcg
     3841 ctgcggcgtg cataggggtc cgccttgagc gtgcactggc ctgccgcgtc cagtacctca
     3901 aatttgtaca gggcacccgt acgcacgccc ggaaggaaaa tttcccatac cccgcattcc
     3961 acacgccaac gcatggggtg aagccggcca tcccagccat tgaaatcacc gaccaccgac
     4021 acccgctgtg catggggtgc ccatacagca aagactgttc cctgcacccc ttccagggtc
     4081 accgggtgtg cgcccagctt ttgccagggg cgcaagtgct ttccttccgc cagcagccag
     4141 acgtcgacat ctccgagcca tggcgcgaag cggtaagggt cctcttgcac caggtggtgc
     4201 ccgggataat ccaccgccag ccggtaatcg cccgcggagc cagggggcag atggcctacg
     4261 aacaagccgc catcgccgct ggcaagcaga gcagccagaa cctcgccagt tccgcggtca
     4321 acggctgaaa caccacgcgc ccccggcagg tacacccgca acgtgatgcc atcagaatcc
     4381 gatttccggt ggggtcccag gtggctgaag ggatcgcggc agcgaccttg ctgcaactgc
     4441 tgggtgagcg cgtgttcctg atgggaaaaa ggcatggagt gagacggggg catcgagagt
     4501 ggtgtcaacc tattttgtca ccaatgcccc tcggtacggt taccagtgga cggtttgggt
     4561 tccaggtggg atttgagcag gcgccgcgca cctgtcacag ggcgcggggt ctcgcaggca
     4621 tagcggtagg cctgcagcgt gcgctgcgcg cgccccaata ccgtgtcagg tggatagtcc
     4681 gcaccaacca gagcgccgaa gggcacgccg gtgagcaact ccatgccttc gctggccagc
     4741 tcggctccgt aaatgtggaa gcggccttgg gccacggcct ccaccacacg cgggtcgagc
     4801 atcaggtggc gccggttgcg tgccggtatc agcacgccct gctggccgtc cagccccagc
     4861 agttcgcagc tgcggaagta gccttcgatc ttttcattga tgcctcccac cggcagcatc
     4921 tccccgtgct ggttgaccgc gccagtgacc gcaataccct gccggatggg aaggcctgac
     4981 aggctcgaca gcagcgcgta gaactccgca cacgatgccg agtcgccctc cacgccgctg
     5041 tattcctgct cgaacaccac ggcagcgttg agtgccagtg gcgcaatgtg tgcaaacagg
     5101 gccgacaggt agctgtgcag tatcagcacg cccttgtcgt ggatcgggcc ggagagcttg
     5161 acctcgcgtt cgatgttgag caggccttct tctcctgcat aggtgcgccc tgtcacccga
     5221 accgggaagc caaactggta gtcacccagg tccaccacgg tcaggccatt gagctgggcc
     5281 acccgctcgc cacggacttc cagcagccgt tcgccgtcga cgatggtgtc ctgcaggcgt
     5341 tcttccgggt agctgtggcg gtgcatccgg gcctgcatgg ctgcgcgcac gtcctgcgct
     5401 tccaccaggt tggcaccccg gctgcgcgcc atggcactgc tttccatcac gagggcttcg
     5461 gagtgggcaa agatggcgct ttgccgcatc tggtcttcgg cttcgcggtg ggtcacttcg
     5521 atcagcacgg ccactgccgc agcggtgaaa tgggccagac ccaggtggcg gcagatgtgg
     5581 gccacaaaga tggctgtggc actgcacgac tcgggcgtgg ccttgaagct ctctgcaaag
     5641 tccaccttgc agcgaaagcg ccgcgcaaac tcggggtcgc cttcctgcac cgcgtagtag
     5701 tcatcgacgg aggcgatcag cacgatcttt acatccacgt cgatgggctc gggctgcagc
     5761 gacacggcgg cgatgggcgc gtacatcatg ccgggttcct cgatctgcag gcgaccactg
     5821 cgcagaaagc ggcgcagctt ttcccacacc ggctcgtcgg ccagcaggtc gcgcaggtgc
     5881 agcatcagat agccgccatg ggccttgacc aggctgcccg cacggatgcg cgagaaatcg
     5941 gtgaccagca tgtcgtcgtc ggcttcgtac tcgatgctgc caaacagcct gcgaaaaagc
     6001 gggttgtctt ccaggatgac cggtgcacct tccagaccgt ggttgtccac cgccacattc
     6061 acgcggaaat gcgataccac ttcaatcaat gcctgcaggc ggatttcctc gtcctcaccg
     6121 ggttggaaca cctcgatgtt ttccatcacc tcatggtgca cctggtccag gtagctgccc
     6181 agcttcacgg tgtccttgat ctgtttgcgc agcccattgc ggatcagctg catggcgtgg
     6241 tcgagcatgg gcttgacggt ctggcggcgc agggcggcca tcgcctcatt gagcgcctgc
     6301 tccatggcgc ggctcttgtc gaggaagcgg ctgatctcgt tgcgcaaggt cgcctcggcg
     6361 ccgtctatct ccgcgcgttc ctcgcgggtc agtgccatgg ccttgcccgc agtcagcggc
     6421 tcgcccttgg tgtcgcgcag ggtgaacacc atattgccct gctcgcgcat caggacgaag
     6481 ttgtgcgcat ccgcaaaggc actgagctcc acgtaggcgc gctcttcttc ggccttgttg
     6541 atattggcca ggcgctcgct ttccgccttg aagtcgggct cggacagtcg cttgggaata
     6601 tcggattgca ggttctttgc aaactgcccc atcagctggc gcagcaggcg gccttcgccc
     6661 gcgggcaggc gcagtgcccg cgggtgctcc ggttcgtcaa aattgtgcag gtagcacagg
     6721 tcgggaggca tcgggcgttt ggcggcctcg ttgcgcatca tctggcccat cagtgtggag
     6781 cgcccgctgc cgacctcgcc cagcacaaac aggttgtagc cgggctggtc catcgtcagg
     6841 ccaaagcgcg cggcctgttc ggcgcgctcc tggccaatcc agggcagggg atggtgcagc
     6901 agttcggatg tgtcggaaaa acccagggac gcggggtcca cggtgcggcg caggtcggaa
     6961 gcgggaagta tcaaggcagt catggggtca atgctacgat cattccgacc cttcccgcca
     7021 tgtcactctt gcatctctaa gccactgggt gctagaaatc caccgccggg cgtacctgcg
     7081 aagccctgac gaaacgccag cgtcccacat agctggcgca cagcagcgag aggactccac
     7141 ccagcgtgac gatcagcagg ggatggcccc aggcgccacc ataggcgcca aacatgctgg
     7201 ccttgacggc gcgcaccacc caggtcagcg gcaaccaggg gctgatattg gcaaacacgc
     7261 caccactgag ttccaccggc aatacaccgc ccgacgacga cagctgcacc gccagaaaga
     7321 tcatggccag tgccttgcca gcatcgccaa aggcgcgggt gagggcaaat acgatgccca
     7381 ggaaggtcag ggcggtcgca cccagggtta gactgaatgc gaccggatgg acgatcggaa
     7441 ttttcaggat cagcattacc gtggccagca ggatggtggc ctgcgacagc acgatcgccg
     7501 cagggatcgc cagcttgccc agggtctgtg ccgggcgcga aaagaactgc gcgtgccgtg
     7561 gcagcacccg cacgtggatc aggaaggcgg caatgccagc gcccagccac agcgcggcag
     7621 gcagtacatt gggcgcaaat ccgctgccgc tgttcgacac cggagcttcc acctccagca
     7681 ccggcttgac cgagttggcc aggccctgcg cgctgccatc ggggccatcc acgctggacg
     7741 gcagggagtc ggccagcagg tccagaccag tcgtgaggcg gtccacgcct gccttgattt
     7801 tctgggtgcc gtcggccagc actgcagtgc ctgacgccac ggcactggca ccggtatcca
     7861 gctcgtcgag ctgccggtcc tgtggcagct tgaccaccat ggtgcgcacg ccgctgttca
     7921 tggccagcat gccgctggtg agagtgtcca cacccgcgct caactggctg gctccggccg
     7981 ccatacggtg ctgggcattg accgaggcga gcaggccgct atcgagttgc gtggtgccat
     8041 cgagcacctg gtcgagcccg tccaccacgc tggagggcac cagcaggctg gacttggctt
     8101 cgctgcggaa ggtctggact ccttcccgca gctggccgct gccactcttg agttcggcca
     8161 ggcccttgcc cagttccacg tgggcggcgg ccagttcgtc agcgccggtc ttgagacggg
     8221 tgagttctga tgcggaggga tgcctggcgt ccatggtgcg caggccggaa gccagctgct
     8281 tgacgcctcc ggtgagctgt gtcacgccgt cgtagagccg gtcggagccc gtggctgcgg
     8341 cgtacgcccc cttggctgcc tgcttggcac cgccgctgag ttcatgtgca ccagagcgca
     8401 actcgtccac ccccgcccgc aggcggtcga cgctgcgctg cgaacccgct gcattggaca
     8461 gcaccagccg ccagcgccgc tcattgaggg ttgcgttgac ttcgtggccg agctcctggg
     8521 cgaacaccct ggcaatgcgc gcggcttcga agttgttgcc ttccgaggtg aacaccacca
     8581 gcctgccgcc tccggcttcc cagccgggaa tggcgttgga gctgaagtcc ttggggatga
     8641 tgagggcaaa ggcgacggca ccttcgcgta ccaaccggcg ggcctcctgc gggtccgcta
     8701 catcctggaa gccaaaggtc tttttcttgc gcaggctggc cgccacgtcc cagccagcgt
     8761 tgaacacatg ctcgcggtac tccacaccct cgtccaggtt gaccagggcc acaggcaggg
     8821 cgccggcgtg cgctgccggg tcccacacgc tggagaggta gatcacggcg tacaccgatg
     8881 gcatcaccgc cacggccagg gtggccagca gcagcttcgg gaagcgcaca aaaaaccgtc
     8941 cctctgcacg ggcgataaac gcgatctggc ggagcagtct cacaggggtg tccaagtttg
     9001 ggatacttgt gcattctctc ctaccttctg gcaccatgaa ctccaccgcc ccccaatctc
     9061 ccaccgcacc cgccgagccc cggctgacat ccctgtcgca tggcggcggc tgcggctgca
     9121 agattgcgcc gggcgtgctg tcggacatcc tcaagaccac caccgccatg ccgctgccgc
     9181 cgcaactgct ggtgggtatc gagacggccg atgacgccgc tgtctaccag ctcaacgacg
     9241 agcaggcgct gattgccacc accgacttct tcatgcccat cgtcgacgat gcgtttgact
     9301 ttggccgtat cgccgccacc aacgccatct ccgacgtgta cgccatggga ggcaccccca
     9361 tcatggcgct ggccctggtg ggcatgccta tcaacgtgct ctccaccgaa accattggcc
     9421 agatcctggc tggcggtgcg gcggtttgca aggcggcggg tatccccatt gccggcggcc
     9481 acaccatcga ttcggtggag cccatctatg gcctggtggc cctgggcctg gtgcacccca
     9541 agcgcgtcaa gcgcaacgcc gatgcccgcg tgggcgaccg gctgatcctg ggcaagccgc
     9601 tgggcgtagg cattctgtcc gccgcgctca agaaggaaaa actcggcgcc gagggctacg
     9661 cccgcatgat tgcggtgact acccagctca acacgcccgg ccccgatctg gccgcgctgg
     9721 acggtgtgca cgccatcacc gatgtgaccg gttttggtct ggcgggccat ggactggaag
     9781 tggcccgtgg tgccaattgc accgtccata tcgactgggc acgcgtgccg ctgatcgaag
     9841 gcgtggccga cctggccgcg caaggcatgg tcaccggcgc cagcggccgc aactgggcgg
     9901 cgtatggcag ccaggtggcg ctggcgcccg aactgtcacc ggtggaccgc gcactgctga
     9961 gcgacccgca aaccagcggt ggcctgctgg tgagctgtgc acccgaagcg gttgcgcagg
    10021 tgctggaaat tttcagtcgc catggttttg cacacgccac agagattggc agcgtgtcgg
    10081 tcgccgacgc cgaccctgcg gggccgcgca tgcgggtgcg ctgagcgcac gctggatgcc
    10141 agcgccggga tcagccttcc ttgcgaaagc gggcgataaa gcgccggtcg atcc
//
LOCUS       DAOSJM010000036        10140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6446_length_10140_cov_4.392861, whole genome shotgun sequence.
ACCESSION   DAOSJM010000036 DAOSJM010000000
VERSION     DAOSJM010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10140
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6446_length_10140_cov_4.392861"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PKX82_02290"
     CDS             complement(<1..240)
                     /locus_tag="PKX82_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOF51105.1"
                     /translation="MDAQQTLKAIRDQLNTVIVGKPDQVQDCVACLLAGGHLLIEDLP
                     GVGKTTLAHALARLLGLGFSRIQFTSDLLPADITGV"
     gene            282..1235
                     /locus_tag="PKX82_02295"
     CDS             282..1235
                     /locus_tag="PKX82_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOF51106.1"
                     /translation="MNKTGYFYHPDCHKHEMGQGHPECPERLSAIEDRLLASGVRDAL
                     DCREAPQAPITDLELAHGRMHVASLRGLTDGLREEIEAGGPTHAQVDPDTSINVHTWD
                     AALRAAGAAIAATDAVMAGDMENAFCAIRPPGHHACRDKAMGFCFFNNVAVAARYALE
                     RHGLKRVAIVDFDVHHGNGTEDIVAGDQRILMLSFFQHPFYPDGGARSNASNLVNLPV
                     PAYTKGDVVRDLILAHWLPRLEAYKPEMIFISAGFDAHREDDLGQMGLVEADYAWITQ
                     HLKAVAKTHAQGRIVSCLEGGYNLDALARSVEAHVRVLADL"
     gene            1235..2515
                     /locus_tag="PKX82_02300"
     CDS             1235..2515
                     /locus_tag="PKX82_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOF51107.1"
                     /translation="MATNLRVPAPKPIDDFEGWIAAFTQPTVLIELAVLALCVALAWG
                     VVAGLRRAVASEDPRSIWFGRRKVDGVLFPLVLLCLGYAALAVLDRYMGVAVFKVAIP
                     VLVSLVVIRVGVKVLQAAFAQAGWVRLLEHTISWIAWLAMVLWVSGLLPVVLNELDLI
                     TWKVGGTTLSVRNMIEGLLTAGAVLIFTLWISSAIETRLLKSATGGELSLRKAVSNAT
                     RALLMFVGLILALSAVGIDLTALSVLGGAVGVGIGFGLQKLAANYVSGFVILAERSMR
                     IGDTVRVDNFEGVITQINARYTVVRSQAGRESIVPNEMLITNRVENLSLVDSKVNQTT
                     VVTVGYDSDVELVSRLLVEAARAQSRVLVEPEPAAHLSAFGADGLEFTVGYWVADPER
                     GQLNLRSDINRAILTALRAHGIDIPYPQRVVHQR"
     gene            complement(2564..5029)
                     /gene="lnt"
                     /locus_tag="PKX82_02305"
     CDS             complement(2564..5029)
                     /gene="lnt"
                     /locus_tag="PKX82_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOF51108.1"
                     /translation="MKPVALINRPAGRVLSAIVLSGILSGIYVRGGGAWVLGFVMVVP
                     WLRVLDTRETLASSLLNASLMSVAFTAAAFSWFGMAIGNYTQLGAVTGLSVLLVAAPL
                     FQPQFLAFALVRYLVGLRHGPVVRALAAASAWVGMEWIAPKILGDSLGYGLYPSPVLR
                     QVADLGGAAGLTVLLLLANEGIAFALARRPEGLRRMLKPLALAGMVPALMAGYGYLVL
                     SASPAPTGNTLRMGLIQSNLVDYEQRRQEVGAHDVVREVLDTHFAMSYDAVVRHRADA
                     VLWSETAYPTTFAHPKSEVGAEFDGEIQSMVNAAGVPFVFGTYDRDAAGEYNAAAVVT
                     PVTGLMGFYRKTRLFPLTEYVPGWLDGPTLRSWLPWTGAWLPGDGARVFPLRLRDGRE
                     IPVLPSICLDDVDPGLAIAGARLGAQAILTMSNDSWFTHYPQGAELHHTVAAFRSIET
                     RLPQFRVTSNGYSAVIDATGRVVAGSQMGERTLVVGELPVPVPPRTLMVAWGDWVGLA
                     GCLLLAWLTLLAASSRWRKRRGNAGPTPAVATPFPTDAFVLPATARVVTGMLRGFARA
                     SLLWMGAAMLLSDSLRGNSLAQIRLFAGCFLAPEAAAWCVLFAFSARVSVENGMLVLM
                     RGKQRLEVALRDIAAIELWRLPIPGPGVSLRLTSGNRWHYGLALAHPIQLAQALSASG
                     AAPVQEAKATISALYAQARDAMVRGRFDSPWMKFVVFPMLLALPAFHLHQNIAYGGGF
                     GEYYTFGLKAYLVTLALWWAAWTIGVVLSAAALRAVIETGTLLSLLVRPAQTVDVRQM
                     LEHVGLALLYLGLPGWLLLHAGW"
     gene            complement(5026..5508)
                     /locus_tag="PKX82_02310"
     CDS             complement(5026..5508)
                     /locus_tag="PKX82_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51109.1"
                     /translation="MSAWQIQETVPTWKEPAHARPPSAVEPEKDNDPTPDLSSYVARG
                     EKPKMSEVINRLHQAGVYTGLGAFSPPGTRPPMVGLAVPEDFVLPPGYVRHHQATDDG
                     QRIEPILMFAPDFQIFDAQQRPMEMPANRVVPPELAPPGLPIRRIVIPAPVDAAKQAQ
                     "
     gene            complement(5739..6857)
                     /locus_tag="PKX82_02315"
     CDS             complement(5739..6857)
                     /locus_tag="PKX82_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOF51110.1"
                     /translation="MENFTERWHDMMTRIRSALCTLVPVLATLALGSGAVASTLNQNV
                     SWTIDRTGTTAKYRVVAYGDSIYAGYNGSTINAAKFAAPTVKAEYLSALWNADIESVR
                     RTKSGAVASDIYNNKIVAERSYMQATSTRAVTFEMCGNDGLQARTAFKKQTGTCNYSG
                     MDTAVSSCKTYVAAAMDYINANAYAGVKVKVISNLHYPGYAADNVQSTCKDATTGATV
                     NLRNKFLPKIATMNYWMCEYARQKGFKCADNFAQYMAADYDSNGDGQVDSEALRYVAG
                     ESESNYLTRITSTLQATLRDSNTHFVSASSSYDYIQSDDTHPTYTGGTVTAGLLGGTT
                     GSGAPRYTTFTGGKNPIWNQYGHERMGWAVSTYNPATP"
     gene            7181..7930
                     /locus_tag="PKX82_02320"
     CDS             7181..7930
                     /locus_tag="PKX82_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOF51111.1"
                     /translation="MKVLVPVKRVVDYNVKVRVKSDGTGVDIANVKMSMNPFDEIAVE
                     EAVRLKEKGVVTEVIAVSCGVAQCQETLRTAMAIGADRGILVQTDEELQPLAVAKLLK
                     ALVDKEQPGLIILGKQAIDDDCNQTGQMLAALADLPQATFASKIEVADGKANVTREVD
                     GGSETLLVTLPAIVTTDLRLNEPRYVTLPNIMKAKKKQLDTFTPADLGVDVAPRIKTL
                     KVAEPAKRSAGIKVPDVATLVSKLKNDAKVI"
     gene            7952..8884
                     /locus_tag="PKX82_02325"
     CDS             7952..8884
                     /locus_tag="PKX82_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOF51112.1"
                     /translation="MTALVIAEHDNASIKGATLNTVTAAAQCGGDVHVLVAGHNAGAA
                     AAAAAQIAGVAKVIHADGEHLAHGLAENVTAQILAIAANYSHIVFPATASGKNVAPRV
                     AAKLDVGQISDITKVVSADTFERPIYAGNAIATVQTADATKVLTVRTTGFDPAAASGG
                     SAAVETQAALAANAGVAYLGSEIAKNDRPELTAAKIIVSGGRALGSNEKFMEVMTPLA
                     DKLGAAMGASRAAVDAGYAPNDWQVGQTGKIVAPQLYVACGISGAIQHLAGMKDSKVI
                     VAINKDPEAPIFSVADYGLEADLFVAVPELVAAL"
     gene            8949..>10140
                     /locus_tag="PKX82_02330"
     CDS             8949..>10140
                     /locus_tag="PKX82_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF51113.1"
                     /translation="MSYVAPVKDMLFNIEHLANIAQIAQIPGFEDAGLDTAQAVLEEA
                     AKFNEGVLSPLNWEGDKYPSSWHNGVVTATPGFKEAFKQFVDGGWQGLQHPVDFGGQG
                     LPKTIGAACGEMQNSANMSFALCPLLSDGAIEALLTAGSDELKATYLENLVAGKWTGT
                     MNLTEPQAGSDLAAVRTRAEPQPDGTYKVFGTKIFITWGEHDMAENIVHLVLARVTGA
                     PEGVKGISLFVVPKFMVNKDGSLGARNDVHCVSIEHKMGIKASPTAVLQYGDHGGAVG
                     YLVGQENRGLEYMFIMMNAARYGVGVQGIAIAERAYQKAVTFSRERVQSRPVDGSLPS
                     AGAIIHHPDVRRMLMTMRAYVEGCRALASVAASAYDAAHHHPDAEVRKPNQAFYEFMV
                     PLIKG"
BASE COUNT         2022 a         2992 c         3173 g         1953 t
ORIGIN      
        1 aacccccgta atgtctgcgg ggagcaggtc gctggtgaac tggatgcgcg agaacccgag
       61 cccgagcagt cgcgccagcg catgggccag cgtcgtcttg ccgacgccgg gcagatcctc
      121 gatcagcagg tggccgccgg cgagcaggca ggctacgcag tcctgcacct gatccggttt
      181 gcccacaatc accgtgttaa gctgatcgcg aattgccttg agtgtctgtt gtgcatccat
      241 ggcaggacgt tacccgaaaa atttgaacca tgagtgcttt ggtgaacaag accggatact
      301 tttaccaccc cgactgccac aaacacgaga tgggacaggg gcatcccgag tgcccggagc
      361 gtctgagcgc cattgaggac cgtctgctgg ccagcggagt gcgcgatgcc ctcgattgcc
      421 gcgaggcgcc gcaggcccct atcaccgatc tggaactggc tcacgggcgc atgcatgtgg
      481 cgtccctgcg tggcttgacc gacggcctgc gcgaagaaat cgaagccggc ggtccgaccc
      541 atgcgcaggt ggacccggat acctccatta atgtgcacac ctgggacgca gcgctgcgcg
      601 cggctggtgc tgccattgcg gccaccgatg cggtgatggc cggggacatg gagaacgcgt
      661 tttgcgccat ccgtccgcca gggcaccatg cctgccgcga taaggccatg ggtttctgtt
      721 ttttcaacaa cgtggcggtg gcggcccgtt atgccctgga gcggcatggt ctcaagcgcg
      781 tggccatcgt ggatttcgat gtgcaccacg gcaatggcac cgaagacatt gtggcggggg
      841 accagcgcat cctgatgttg agttttttcc agcacccgtt ttatcccgac ggaggcgcgc
      901 gctccaacgc cagcaatctg gtgaatctgc cggttccggc ctacaccaag ggggatgtgg
      961 tgcgcgacct gatcctggcg cactggttgc cccggctgga agcctacaag cccgagatga
     1021 ttttcatcag cgcgggcttt gatgcccacc gtgaggacga tctgggccag atgggcctgg
     1081 tcgaggcgga ctacgcctgg atcacccagc acctcaaggc cgtggccaaa acccatgcgc
     1141 agggacgcat cgtgtcgtgt ctggaaggcg gctacaacct ggatgcactg gcccgcagtg
     1201 tagaggcgca tgtgcgcgta ctggcagatc tgtgatggcg acgaacctga gggtgccggc
     1261 acccaaaccc attgacgact tcgaaggctg gattgccgcc ttcacccagc ccacagtatt
     1321 gatcgagctg gcagtgctgg ccctgtgcgt ggccctggcg tggggtgtcg tggccgggct
     1381 gcgccgcgca gtggcctcgg aagacccgcg ttcgatctgg tttggccgca ggaaggtgga
     1441 tggcgtgctg ttcccgctgg tgctgctgtg tctgggctac gcggcgctgg cggtgctgga
     1501 ccggtacatg ggcgtggcgg tgttcaaggt ggccattccg gtgctggtgt cgctggtggt
     1561 gattcgtgtc ggagtgaagg tattgcaggc ggcgtttgca caggcggggt gggtgcggct
     1621 gctggagcac accatttcct ggatcgcgtg gctggccatg gtgctgtggg tcagcggcct
     1681 gctgccggtg gtgctcaatg agctggacct gatcacctgg aaggtgggcg gaaccacgct
     1741 gtcggtgcgc aacatgattg agggcttgct gaccgcgggg gcagtgctga tcttcacgct
     1801 gtggatttcg tcggcgatcg agacgcgcct gctcaagtcg gctacgggcg gggagttgtc
     1861 cttgcgcaag gcggtgagca atgcgacgcg cgcgctgctg atgtttgtcg gactgatcct
     1921 ggctctgtcg gccgtgggca tcgacctgac ggcgctgtcg gtgctgggtg gcgcggtggg
     1981 tgtgggtatc ggctttggcc tgcagaaact ggcggccaac tatgtgagtg gcttcgtgat
     2041 cctcgccgag cgcagcatgc gcattgggga caccgtgcgg gtcgataact ttgaaggggt
     2101 gatcacccag atcaacgcgc gctataccgt ggtgcgctcc caggcgggca gggagtccat
     2161 cgttcccaat gaaatgctga tcaccaaccg ggtggaaaac ctctcgctgg tcgattccaa
     2221 agtgaaccag acgacggtgg tgaccgtggg ttacgactcg gacgtggagt tggtgtcgcg
     2281 cctgctggtg gaagccgcgc gcgcccagtc gcgcgtgctg gttgaaccgg aaccggctgc
     2341 acacctgtcg gcgtttggcg ccgatgggct ggagttcacg gtgggctact gggtggcgga
     2401 cccggaaagg gggcaactga acctgcgctc ggacatcaat cgggccattc tgaccgcgct
     2461 acgcgcgcat ggcattgata tcccgtaccc gcagagggta gtgcaccaac gctgatcccg
     2521 cgaggctgcc agtgctgcgc ggtggatgca tgcatccgtg catttaccag cccgcgtgca
     2581 gcagcagcca gcctggaagt ccgaggtaca gcaaggccag gccaacgtgt tccagcatct
     2641 gacgaacgtc gaccgtctgg gcgggccgca ctagcagtga cagcagcgtt ccagtttcga
     2701 tcaccgcgcg cagtgccgca gcactcagta cgacgccaat ggtccatgcc gcccaccaga
     2761 gtgcaagggt gacgagatac gctttcaggc caaaggtgta gtactcgccg aaacctcccc
     2821 cataggctat gttctggtgc agatgaaaag cgggaagtgc caacagcatg gggaacacca
     2881 cgaatttcat ccatgggctg tcgaagcgcc ctcgcaccat ggcgtcacgt gcctgcgcat
     2941 agagcgccga aatggtcgct ttggcttcct gcacgggtgc cgctccgcta gcagacagcg
     3001 cttgggccag ctggataggg tgcgccagcg ccaggccgta atgccatcgg ttcccggaag
     3061 tcagtcgcag tgatactcca ggcccgggaa tgggcagtcg ccatagttcg atagcggcaa
     3121 tgtcccgcag tgcaacttca agccgctgct tgccccgcat gagaaccagc atgccgtttt
     3181 ctacggacac cctggcggaa aaagcaaata gcacgcacca ggccgccgct tccggcgcga
     3241 ggaaacagcc ggcgaagagc cggatttggg ccagtgaatt cccgcgcagg gaatcgctga
     3301 gcagcatggc cgcacccatc cacaagagac tggcgcgcgc aaaaccgcgc aacatgcctg
     3361 tcaccacgcg tgccgtggct gggagaacga aggcgtcggt cgggaagggc gttgcaacgg
     3421 ccggtgtcgg cccggcattc ccccggcgct tgcgccaccg ggacgatgcc gccaggagtg
     3481 tcagccacgc gaggagcaga cagcctgcga gcccaaccca atccccccag gccaccatca
     3541 aagtgcgtgg tggtaccggg acgggcaact caccgaccac cagcgtgcgc tcgcccatct
     3601 gcgacccggc aaccaccctg cccgtggcgt caatcacggc gctgtagccg ttggatgtga
     3661 cccggaactg cggcaggcgt gtttcgatac tgcggaaggc tgccaccgtg tggtgcaact
     3721 cagctccctg tggatagtgg gtgaaccagg aatcgtttga catggtcaga atggcctgcg
     3781 cccccagtcg cgcgcccgca atcgccagtc ctgggtccac gtcgtccaga cagatggatg
     3841 gcagtacggg gatttcgcgg ccgtcacgga gtcgcaatgg aaaaacacgt gcgccatctc
     3901 ccggcagcca tgcgccagtc cacggcagcc agctccgcag cgttggcccg tccagccagc
     3961 ctggcacata ctcagtgagg gggaaaagac gtgtctttcg gtagaagccc atcaggcctg
     4021 tgaccggggt gacaactgcg gccgcgttgt attctccggc ggcgtcgcga tcataggttc
     4081 caaaaacaaa tggcacaccg gcagcgttga ccatgctctg tatctcaccg tcgaattctg
     4141 cgccgacttc acttttgggg tgtgcaaacg ttgtgggata ggcggtctct gaccacagga
     4201 cggcatccgc acgatgacga acgaccgcgt catagctcat cgcaaaatgc gtatccagca
     4261 cttcccgcac aacgtcatgg gcgcctactt cctgccgtcg ctgttcgtag tccactaaat
     4321 tggattggat caaccccatc cgcagtgtgt tgcccgtggg cgcagggctt gcagaaagca
     4381 ccagataacc atagcctgcc atcaatgcgg gcaccattcc cgcaagcgcc agaggcttga
     4441 gcattctgcg cagtccttcc ggccggcgcg caagtgcgaa agcaatccct tcgttcgcaa
     4501 gcagcaacag gacggtcagc ccagcagccc ccccaaggtc agcgacttgg cgcaggaccg
     4561 gtgacgggta gagcccgtag cccaatgagt cgcccagaat tttgggcgca atccactcca
     4621 tacctaccca ggctgaggct gctgccagcg cacgcacaac gggtccatgc cgcagtccga
     4681 ccaggtagcg taccaatgca aacgccagga actgcggctg aaagagtggc gctgccacca
     4741 gcagaacgga cagcccggtt actgcgccaa gttgggtgta attcccgatc gccataccaa
     4801 accaggaaaa agcggcggcc gtgaacgcca cggacatgag ggaggcattg agaaggctgc
     4861 tggcgagcgt ctctcgtgta tccaggaccc tgagccaggg cacgaccatg acaaagccga
     4921 gcacccatgc gccacctcca cgcacgtata tccccgacaa aataccgcta aggacaatag
     4981 ctgacagcac acgccccgct gggcggttga ttagggccac cggcttcatt gggcctgctt
     5041 ggcggcgtca acgggtgcag ggatcacaat gcggcggatt ggcaaaccgg gcggggcaag
     5101 ctctggcgga acgacgcggt tggcaggcat ttccatggga cgttgctggg catcaaagat
     5161 ctggaaatcc ggcgcaaaca tcagaatcgg ttcaatgcgt tgcccgtcgt cagtcgcttg
     5221 gtggtggcga acataacctg ggggcagcac aaagtcttcc ggcacggcca aaccgaccat
     5281 gggtgggcgt gttccgggag gcgaaaacgc gccaagcccg gtatacacac ctgcctggtg
     5341 caatcggttg atcacctccg acattttggg tttctcgccg cgggccacgt agctggacag
     5401 gtctggtgtc gggtcattgt ctttctcggg ctccactgcg cttggtggtc tggcatgggc
     5461 cggttccttc catgtgggaa ccgtttcctg aatttgccag gcggacacag cagggggggg
     5521 cgtggtgacg gccgccggta gcggcaattt cacattcgca gagacctcag ccggcaactc
     5581 cggcgaaaaa aggaagtagg ccagccctcc tccagaaatc accgcagcaa caagaaggaa
     5641 accccaattg ggggtccagg caggtgcccc atttgcggct tccggtggcg gggccttgcg
     5701 ctggcgtcgt gtaaccaaga gctgttgggc ctaatccatc acggggtcgc tgggttgtac
     5761 gttgagaccg cccaacccat gcgttcatga ccgtattggt tccagatggg gttctttcca
     5821 cctgtgaacg tggtgtagcg cggtgcaccc gatcctgtgg tgccgcccag tagacccgct
     5881 gtgaccgtgc caccggtgta agtaggatgg gtgtcatcag attgaatgta gtcgtagctg
     5941 ctgctggcgg acacgaagtg tgtattggag tcacgcaagg ttgcctgcag tgtgctggtg
     6001 atgcgcgtca gatagttcga ctcgctttcg ccggcaacat aacgcaacgc ctccgagtcc
     6061 acttgtccat cgccgttgct gtcgtaatct gccgccatgt actgggcaaa gttatccgcg
     6121 cacttgaagc ctttctgccg cgcgtattca cacatccagt aattcatagt cgcgattttg
     6181 ggaagaaact tgttacgcaa attgacggtg gcgccggtgg tcgcatcctt gcaggtgctt
     6241 tgcacgttgt cggctgcata gcccgggtaa tgcaaattgg atatgacttt gactttcacg
     6301 cctgcatagg cattggcatt gatgtagtcc attgcagcag caacataggt cttgcaactg
     6361 ctgaccgcag tgtccatgcc gctgtagttg caggtgccgg tttgtttttt gaaggccgta
     6421 cgtgcttgaa ggccatcatt gccacacatc tcaaacgtca ccgcccgggt gctcgttgcc
     6481 tgcatataag agcgttctgc gacgatcttg ttgttgtaaa tatccgaggc cactgcgcca
     6541 gatttggtgc gccgcacgct ttcaatatct gcgttccaaa gtgcagacag gtattcggcc
     6601 ttgacggtcg gtgcagcgaa cttagcggcg ttgatcgtgg agccgttgta gccagcgtag
     6661 atggaatcac cataggcaac tacgcggtat tttgcggttg ttcctgtccg gtcaattgtc
     6721 caggagacgt tttggttcaa ggtgctggcg acagcaccag aaccgagtgc caacgtggca
     6781 aggacgggca ccaatgtgca caatgcagaa cgaattcgag tcatcatgtc atgccacctt
     6841 tcggtaaagt tttccatgga atcagcgcag tcaaaccagt gtttggtacc tcagtgtctc
     6901 agctttgacg ggatcgccca taggagtaac cctgccggga aaaccctatg gatttggcca
     6961 ttgcgacgca gcgggggggc tgctgagaag caggctgacg cgcattagac acggcactcc
     7021 agaatcgggg tggcgctggg ctataatcca aaaaagcacg atcgtactat tttatctttg
     7081 caccgttttt ccgaatggaa aggccggtcc gcatagaatc gaacagttga cgtgcacgtc
     7141 aagtggattt ccctgtttat aactatctgg agtcgcagca atgaaagtcc tggtccccgt
     7201 aaaacgtgtg gtggactaca acgtcaaggt ccgagtcaag tcggacggta cgggtgtaga
     7261 catcgccaac gtcaagatga gcatgaaccc ctttgacgaa atcgcggtgg aagaagccgt
     7321 acgtctgaag gaaaagggcg tggtcactga agtgatcgcc gtttcctgcg gcgtggccca
     7381 gtgccaggaa accctgcgta ccgccatggc catcggtgca gaccgcggca ttctggtgca
     7441 gactgacgaa gaactgcagc ccctggctgt ggccaagctg ctcaaggccc tggtggacaa
     7501 ggaacaaccc ggcctcatca tcctgggcaa acaggctatc gatgacgact gcaaccagac
     7561 tggtcaaatg ctggctgccc tggcagacct gccccaagcg actttcgcca gcaagattga
     7621 agtggctgac ggcaaagcca atgtcacccg tgaagtcgac ggtggttccg agacgctgtt
     7681 ggtgacgctg cccgccatcg ttacgaccga tctgcgcctg aatgagcccc gctatgtgac
     7741 cttgcccaac atcatgaagg ccaagaagaa gcagctggac accttcaccc ctgccgatct
     7801 gggcgtggat gtggctcccc ggatcaagac cctcaaggtt gccgagcccg ccaagcgcag
     7861 tgccggtatc aaggtgccgg acgtggcaac gctggtgtcc aagctcaaga acgacgccaa
     7921 agtcatctaa gaagaacaag gaaccatcaa catgaccgct cttgttattg ccgaacacga
     7981 caacgcgtcc atcaaaggcg cgaccctcaa taccgtgacc gcagctgccc agtgtggcgg
     8041 tgatgtgcac gtgctggtgg ctggccacaa tgccggcgca gctgctgcgg ctgctgccca
     8101 gatcgctggc gttgccaagg ttatccacgc cgatggcgag catctggccc atggcttggc
     8161 agaaaacgtg actgcccaga ttctggcgat tgctgcaaac tacagccaca tcgttttccc
     8221 tgccaccgca tcgggcaaga acgtggctcc gcgcgtggct gccaagctcg acgtgggcca
     8281 gatctccgat atcaccaagg tggtgagcgc cgacaccttc gagcgcccca tctatgctgg
     8341 taacgcaatt gccaccgtgc aaactgccga tgccaccaag gtgctgaccg tgcgtactac
     8401 cggctttgat cctgctgcag cttctggcgg cagcgctgcc gttgaaaccc aggcagcctt
     8461 ggccgccaac gctggcgtgg cctatctggg ctccgagatc gccaagaacg accgcccaga
     8521 actcactgct gccaagatca tcgtgtctgg cggacgtgca ctgggttcca acgaaaagtt
     8581 catggaagtc atgacgcccc tggccgacaa gctgggtgcc gccatgggtg cttcccgtgc
     8641 cgctgtggac gcaggctatg cgcccaacga ttggcaggtg ggccagaccg gcaagatcgt
     8701 ggcgccccag ctgtatgtgg cgtgcggcat cagcggtgcg atccagcacc tggccggcat
     8761 gaaggactcc aaggtgatcg ttgcgatcaa caaggacccg gaagccccga tcttctccgt
     8821 ggccgattac ggtctggagg ccgatctgtt tgtggccgtg ccagagctgg tcgcggcgct
     8881 gtaatcgcca aacgaataag ggcatcgcgt gcgatgccct ttttttattt ccccattcgg
     8941 agttcttcat gagttacgtc gcccccgtca aagacatgct gttcaacatc gagcatctgg
     9001 ccaacattgc gcagattgcc cagattcccg gtttcgaaga tgccggcctg gacacggcgc
     9061 aggccgtgct ggaagaggcg gcgaaattca atgaaggcgt gctgtcgccc ctgaactggg
     9121 aaggcgacaa atacccgtca agctggcata acggtgtggt caccgccacc cccggtttca
     9181 aggaagcctt caaacagttt gtggacggtg gctggcaggg gctgcaacac ccggtggact
     9241 ttggtggcca gggtctgccc aaaacgattg gcgcagcctg cggtgaaatg cagaactctg
     9301 ccaacatgag ctttgcgctg tgcccgctgc tgagcgacgg cgctatcgaa gccctgctga
     9361 cagccggttc ggacgaactc aaggccacct acctggaaaa cctggtggcg ggcaagtgga
     9421 ccggcaccat gaacctgacc gagccccagg ccggcagcga tctggcggcg gtgcgcaccc
     9481 gtgccgaacc ccagcccgat ggcacctaca aggtgttcgg caccaagatt ttcatcacct
     9541 ggggtgagca cgacatggcc gagaacatcg tccacctcgt actggcccgc gtgaccggtg
     9601 cacccgaagg tgtcaagggc atcagcctgt tcgtggtgcc caaattcatg gtcaacaagg
     9661 atggctcgct gggggcgcgc aatgacgtgc attgtgtgag catcgagcac aagatgggca
     9721 tcaaggcgtc gccgacggcg gtgctgcagt acggtgacca tggtggtgcc gtcggttatc
     9781 tggtgggtca ggaaaaccgc ggcctcgagt acatgttcat catgatgaac gctgcgcgct
     9841 acggcgtggg tgtgcagggc attgcgattg ccgagcgtgc ctatcagaag gccgtaacct
     9901 tctcgcgcga gcgcgtgcaa agccgcccag tggacggctc cctgccaagc gctggcgcca
     9961 tcattcacca ccctgacgtg cgccgcatgc tgatgaccat gcgtgcctat gtggaaggtt
    10021 gccgtgcgtt ggccagcgtc gcggcttctg cctacgatgc agcccaccac caccccgatg
    10081 ccgaagtgcg caagcccaac caggccttct atgaattcat ggtgccgctg atcaagggct
//
LOCUS       DAOSJM010000037        10039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6524_length_10039_cov_5.229067, whole genome shotgun sequence.
ACCESSION   DAOSJM010000037 DAOSJM010000000
VERSION     DAOSJM010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6524_length_10039_cov_5.229067"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PKX82_02335"
     CDS             <1..538
                     /locus_tag="PKX82_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51114.1"
                     /translation="SGSILDRNGGSVCLRNQHSIEFATRVAHSAHEALGDMFIEGDEV
                     AFRRHIQGLLAQGDVPVQKRIATLFAFWSNIDSLGDQFQVGPEHMALGINLRDRKFSI
                     LNELMALCPWVIHRVIGVKPEIKGSGEIEMFKAILGFVDQQCREQRLNDRDSVELLFK
                     MLGAWAQKEQAGQVSRSS"
     gene            complement(778..1290)
                     /locus_tag="PKX82_02340"
     CDS             complement(778..1290)
                     /locus_tag="PKX82_02340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF51115.1"
                     /translation="MSTLVIIPGWRDSGPGHWPSLWAERLPGAVRVQQDDWITPSRNA
                     WVASIARTILEQPEPVVIAAHSLGSIATTHLPAEAANHIKGALLVAPADPERRGVLAD
                     FAPVPYQPLPYRSILVASSNDPYCPVRTAGAYARAWGSEFVRLQNAGHINVEAGFGEW
                     PLGLASLQSL"
     gene            complement(1402..2697)
                     /locus_tag="PKX82_02345"
     CDS             complement(1402..2697)
                     /locus_tag="PKX82_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF51116.1"
                     /translation="MSAHWKFETKLVHAGYSPDPTTKAVAVPIYQTAAYAFDSAQHGA
                     DLFDLKVAGNIYTRIMNPTNDVLEKRVAALEGGIGALALASGQAAVTYAIQTIAEAGD
                     NIIASTALYGGTYNLFAHTLPQFGITTRFADPRNLASFEGLFDDKTKAVFIESVSNPQ
                     GAVTDIAAIAAIAHRHGVPLIVDNTVASPYLLRPIEHGADIVVHSLTKYLGGHGTTIG
                     GAIVDSGKFPWAEHKAKFKRLNEPDPSYHGVVYTEALGPAAYIGRARVVPLRNTGAAL
                     SPFNAFLILQGIETLALRMDRINDNTLRVAQFLQSHAKVVWVSYAALPSHPDHALAKK
                     YLGGKASGLLTFGVKAAAGQGREAGARFLDGLQVFTRLVNIGDAKSLATHPASTTHRQ
                     LSPEELAKAGVPEDAVRLSVGIEHIDDLLADLEQALAAV"
     gene            complement(2788..3798)
                     /locus_tag="PKX82_02350"
     CDS             complement(2788..3798)
                     /locus_tag="PKX82_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF51117.1"
                     /translation="MTIRRHLLSIVATAALASTALGAFAQQSITLLNVSYDPTRELYA
                     EYNPLFIKHWKAKTGQDVTIKQSHGGSGKQARGIIDGLEADVATLALAGDTDALHNNG
                     GYIPKDWQKRLPLASSPYTSTIVLVVRQGNPKGIKDWDDLIKPGVSVITPNPKTSGGA
                     RWNYLAAWEFAKRKYGGDAKAKEFITRLYANVPVLDTGARGSSITFAQRNQGDVFISW
                     ENEAYLLEKEFGNKVDFVYPSLSILAQPAVTVVDKNVDRKGTRAVAEEYLKFLYTEEA
                     QDIIGKNFYRPTSAKAQAKYAKQLPKVNLFTIDEAFGGWDKAAKDHFADGASFDQIYA
                     RK"
     gene            complement(4128..5369)
                     /locus_tag="PKX82_02355"
     CDS             complement(4128..5369)
                     /locus_tag="PKX82_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51118.1"
                     /translation="MSAILQKLYVRIWLAVVLAVAVLTLLVGWAWRATTEPPLREVVV
                     RNAQGDVIGAGRMRLGRRPDAEAPRTAPPSRAASSPADDDADEPPSRFGRGPEFLVQM
                     QDGQTVHMHLPRPPTSTWRAPFGFFWTLGLVAIAVALATYPIVRRLTRRLEALQRGVE
                     QWGDGNLSARVPEGGDDEVGFLATRFNHAAQRVETLLKSRDALLASQKSLLANASHEL
                     RSPLTRIRMGLELMGPASSSARDEIARNIAELDQLIEEILLASRLDAKEADLGTVEPV
                     DLVGLSAEECARHQAVLETTQASLVVPGVAKLLRRVVRNLLENARRYGTGEVTLTLLQ
                     DGNFAVIQVSDRGPGVPEALQARIFEPFYRLPGASEREGGVGLGLALVKSITQRHGGT
                     VQCSNRDGGGACFEVRLPQNS"
     gene            complement(5382..6098)
                     /locus_tag="PKX82_02360"
     CDS             complement(5382..6098)
                     /locus_tag="PKX82_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF51119.1"
                     /translation="MHQHLLMIEDDARLAAMVAEYLTQNGFTVSHAADGLSGLDQLAQ
                     PGHGQPPDLVVLDLMLPDIDGLEVCRRIRSLPTAMAQTPILMLTAKGDPMDRIIGLEM
                     GADDYLPKPFEPRELLARIRAVLRRKVEAPAATTQTLRFGSLEIDRDARTVSVQGALC
                     ELTSYQFDLLVALAERAGRVLTRDQIMEAVRGRELEAFDRSIDVHMGRIRAAIEADAK
                     DPKRILTVRGVGYVFARQQD"
     gene            complement(6221..7546)
                     /locus_tag="PKX82_02365"
     CDS             complement(6221..7546)
                     /locus_tag="PKX82_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF51120.1"
                     /translation="MSAPTPSPAPTTTPITWAQTLKVYAEPASLRMLSLGFSAGLPLL
                     LVFGTLSFWLREAGIDRTTIGFLSWVGLAYGFKWVWSPLVDRMPIPVLTRLLGRRRSW
                     LLASQAVIMAALVVMATTDPKLALAPVVWCAVLVAFGSATQDIALDAFRIESADTQHQ
                     AALAATYQTGYRLAMIWSGAGALWIAARSEVPNSALYQQGAWQTAYLVMAASMLPGVL
                     TVLFSREPVARALPQARNAMEWLRGALVEPFAEFITRYRWHAVLILALIATYRISDVV
                     MGIMANPFYVDMGFTKDEVAAVTKIYGVVMTLVGAFLGGALALRMGVMRVMMLGAILS
                     AGTNLLFAWLSGQGHDLHALIFVISADNLASGIASAAFIAYLSSLTNVNYSATQYALF
                     SSMMLLLPKFVAGYSGMYVDAFGYGSFFTSTALLGAPVLLLVWLTSRIK"
     gene            7566..8384
                     /locus_tag="PKX82_02370"
     CDS             7566..8384
                     /locus_tag="PKX82_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOF51121.1"
                     /translation="MNTLPLKLSLALALAFPWNLAAVHARNGVEVGGNSAFTNLVPAE
                     SVERSAAQEYGQMLQQAASKNALGGKDHPQVKRLRAIAQRIIPHAMEWNPRAREWKWE
                     VNLMGSSQINAFCMPGGKIAFYSGILDKLKLTDDEVAMVMGHEIAHALREHARERMGK
                     NAATGIGANVLSQLLGLGQVGQTVTQYGAQLLTLQFSREDESEADLVGMELGARAGFD
                     PRAGITLWQKMSAAAKGAPPQWLSTHPSGSTRIAEMEANLPRVMPLYQKARAAR"
     gene            complement(8406..8627)
                     /locus_tag="PKX82_02375"
     CDS             complement(8406..8627)
                     /locus_tag="PKX82_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HOF51122.1"
                     /translation="MFPEYRELITQLKTTDHHFTRLFDQHNALDQKIKNKENHVEPGT
                     PDEIENLKKEKLHLKDQLYAILKKASGAH"
     gene            complement(8659..9051)
                     /locus_tag="PKX82_02380"
     CDS             complement(8659..9051)
                     /locus_tag="PKX82_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF51123.1"
                     /translation="MTILVAYVPRPEGQAALDKGIEIAKRRNERLLVVNATTGGAKDE
                     GAMASVQDVERIEAQLKAAGVQSEFKQFGRGKSAIEEIEVLVTALQPYVLVIGLRKRS
                     AVGKLILGSVAQEILMSVDCPVLCVKAH"
     gene            complement(9124..9915)
                     /locus_tag="PKX82_02385"
     CDS             complement(9124..9915)
                     /locus_tag="PKX82_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOF51124.1"
                     /translation="MFKLTSLNLNGIRSATSKGLVAWLEKAQPDCICVQEVKAQAPDV
                     EGKFEELAGLKGYFHFAQKKGYSGVGAYTRLEPSDVIVGFDGDEFDAEGRWVELRFDT
                     PKRKHSLISCYFPSGSSGEDRQQAKFRFLDKMYPHLQALKAERDFILCGDVNIAHREA
                     DLKNWRSNQKNSGFLPEERAWMTRLTSEGGIVDVYRQLEPDTTDTCYTWWSNRGQAYA
                     NNVGWRLDYHLATPALAAGAKTADIYKGEKFSDHAPITIAYDFNA"
     gene            9926..>10039
                     /locus_tag="PKX82_02390"
     CDS             9926..>10039
                     /locus_tag="PKX82_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOF51125.1"
                     /translation="MSVQDPVQDPQHDVLAQEFVEFAVQCGVLRFGEFKTKA"
BASE COUNT         1857 a         2905 c         3315 g         1962 t
ORIGIN      
        1 aagtggctca atattggacc ggaatggtgg atcagtttgt ctccggaatc aacactctat
       61 cgaatttgct acccgcgtgg cccacagcgc acacgaggcc ctgggtgaca tgttcatcga
      121 aggcgatgag gtggcctttc gtcgccacat ccaagggctg ttggcccagg gcgatgtgcc
      181 ggtgcaaaaa cgcatcgcca ccttgtttgc cttctggtcg aacatagaca gcctgggaga
      241 ccagttccaa gttggccccg agcacatggc gctggggatt aatttgcgtg atcgcaagtt
      301 ctccatcctt aacgagctga tggcgctgtg tccttgggtg atccaccgag tcatcggcgt
      361 gaagccggag atcaagggca gcggtgagat tgagatgttc aaggccatcc tgggctttgt
      421 cgatcagcag tgccgcgagc agcgcctgaa cgatcgcgat tcggtggagt tgctgttcaa
      481 gatgctaggt gcttgggcgc aaaaggagca agccgggcag gtatcgcggt cgagttgacg
      541 cctgtactcg gccgagaatc ttgcgtcgca ctggtgtgtc cgtgtgacta gtccacattg
      601 gcgcaatacc aaatcaagag ttcgccctgg ctggcgcgac gcagttgcga gtcagttccg
      661 ctccacactc gataccagtc acccgaacca gtgcttcggg attgcggtga ttgactgcaa
      721 ccgctgcaaa ccggaaagta aatattccgg gagacattgt gggcctcagg cgttccgtca
      781 aagcgactgc agcgacgcca gtcccaacgg ccattcgcca aaaccggctt ccacgttgat
      841 gtggccggcg ttctgcaggc gcacaaattc actgccccac gcgcgggcgt aggcaccggc
      901 ggtgcgcact gggcagtacg ggtcgttgct gctggccacc aggatgctgc ggtagggcag
      961 cggctggtag ggcaccggtg caaagtcggc cagcacgccg cggcgctcgg ggtcggccgg
     1021 tgccaccagc agggcgccct tgatgtggtt ggcagcctct gcgggcaggt gggtggtggc
     1081 gatgctgccc aggctgtgtg cggcgatcac cacaggctca ggctgttcaa ggatggtgcg
     1141 cgctatggac gcaacccatg cgttgcggga cggcgtgatc cagtcatcct gctgcacgcg
     1201 cacggctcct ggcagccgtt cagcccagag gcttggccag tggccaggac cggaatcgcg
     1261 ccacccggga atgatgacaa gggtggacat gcgagtatcg tgatgcgcct gaagaaaggg
     1321 actgcattgt gtaaatgcat cccctccatc tccaactaca tatttcccat ttagttattg
     1381 caaagtcgta cgagctggtg gctagacggc ggccagtgcc tgctccagat cggccagcag
     1441 gtcgtcaatg tgctcgatgc ccacggacag gcgcactgca tcttccggga caccggcctt
     1501 ggccagctcc tcgggcgaga gttgccggtg ggtggtggac gcagggtggg ttgccagtga
     1561 tttggcatcg ccaatgttga ccagccgtgt gaacacctgc aggccatcga ggaaacgtgc
     1621 gcccgcttcg cggccctggc ctgcggcggc tttcacgccg aaggtcagca ggcccgaagc
     1681 cttgccgccg aggtacttct tcgccagggc gtggtccggg tggctgggca gggccgcgta
     1741 gctgacccag accaccttgg cgtggctctg caggaactgc gctacgcgca gggtgttgtc
     1801 gttgatgcgg tccatgcgca aggccagggt ctcgatgcct tgcaggatca ggaaggcgtt
     1861 gaagggcgag agcgctgcac cggtgttgcg cagcggaacc acgcgcgcgc ggccgatgta
     1921 ggcggccggc cctaatgctt cggtgtagac cacaccgtgg tagctgggat cgggctcgtt
     1981 caggcgtttg aatttggctt tgtgctctgc ccaggggaac ttgccgctgt ctacaatcgc
     2041 accgccaata gtggtgccgt ggccgcccag gtatttggtg agcgagtgca ccacgatgtc
     2101 tgcgccgtgc tcgatggggc gcagcaggta gggtgaggcc acggtgttgt ccacaatcag
     2161 tggcacgccg tggcgatggg ctatggccgc aatggcggca atgtcagtca ctgcgccctg
     2221 tgggttgctc acagactcga tgaacacggc cttcgtttta tcgtcaaaca gaccttcaaa
     2281 gctggccagg ttgcgcggat cggcaaagcg ggtggtgatg ccgaactgcg gcagcgtgtg
     2341 ggcaaacagg ttgtaggtgc cgccatacag cgcggtgctg gcgatgatgt tgtcacccgc
     2401 ctcggcaatc gtctggatgg cgtaggtcac cgcggcctgg ccggaagcca gcgccagtgc
     2461 gccaatgccg ccttccaaag cagccacgcg cttttccagc acgtcgttgg tcgggttcat
     2521 gatgcgggtg tagatgttgc cggcaacttt caggtcgaac agatcggcac cgtgctgggc
     2581 gctgtcaaag gcataggcgg ccgtctggta gatgggcact gctaccgctt tggtggtggg
     2641 gtcgggcgag tagccggcgt gaacgagctt ggtttcaaat ttccagtggg cggacatggc
     2701 gagtctctct ttcaaagtgg gatgaatggg tctgcgctac caatccagta gctggttgcg
     2761 gagtatgcac gggggctgga agccgattta cttcctggcg tagatctggt cgaagctggc
     2821 gccgtcggca aagtggtctt tggctgcctt gtcccagccg ccaaaggctt cgtcaatggt
     2881 gaacaggttg accttgggta actgcttggc gtacttggcc tgcgccttgg cactggtggg
     2941 gcggtagaag ttcttgccaa tgatgtcttg tgcttcttcg gtgtacagga atttcaggta
     3001 ctcctctgcc acggcacggg tgcctttgcg gtccacgttc ttgtccacca cggtcacggc
     3061 aggttgggcc aggatgctca gggaggggta gacaaagtcc accttgttgc cgaactcctt
     3121 ttccagcagg taggcctcgt tttcccagga gatgaagaca tcgccctggt tgcgctgggc
     3181 gaaggtgatg gaggagccgc gggcgccggt gtccagcacg ggcacgttgg cgtacagcct
     3241 ggtgatgaat tccttggctt tggcatcacc gccgtacttg cgcttggcga attcccacgc
     3301 agccaggtag ttccatcgtg cgccgccgga ggttttggga ttgggcgtga tcacgctgac
     3361 tccgggcttg atcaggtcat cccagtcctt gatgcccttg gggttgccct gacgcaccac
     3421 caggacgatg gtggaggtgt agggagagga cgccagaggc aggcgttttt gccagtcttt
     3481 gggaatgtag ccaccgttgt tgtgcagcgc gtcggtatcg ccagccaggg ccagggtggc
     3541 gacatcggct tccaggccgt caatgatgcc gcgcgcctgt ttgcccgaac cgccgtggct
     3601 ttgcttgatg gtcacgtcct ggccggtctt ggctttccag tgcttgatga agagcgggtt
     3661 gtattcagcg tacaactcgc gcgtggggtc gtaagacaca ttgagcaagg tgatggattg
     3721 ctgggcgaag gcgcccagcg cggtgctggc cagggcagcg gtggcaacga tgctcagaag
     3781 atggcgacgg atggtcatga aatactccag aaaaatgcga cgcaaaattg cgatggattg
     3841 gattctggaa gtgagtcgtt aaaactggaa ctactgatgt tttggtttta aataaccaaa
     3901 acatctaaat aagtcatttc tcagaccaca gggcgaaaaa aaagccaccc ggaggtggct
     3961 ttgtaaagtc cccgaaggga cgtcgttgga acctgtgaag ccagcctatt gggtggttga
     4021 tcttcgatcc ttgtgtactg tgacagcagc tggatgcaac tgtagaccgc gccgcagatg
     4081 ggcggtatcc cccattcggg tgagtttgtg agtttttttg caaaaactca gctgttttgc
     4141 ggcaatcgga cctcaaaaca ggcaccgcca ccatcgcggt tgctgcattg gaccgttccg
     4201 ccgtggcgtt gggtaatgga tttgactagt gccaggccca ggccgacacc cccttcgcgt
     4261 tcggatgcgc cgggcaggcg gtaaaacggc tcaaaaatgc gcgcctgcaa cgcttcgggc
     4321 acgccaggtc cacggtcgct gacctggatg acggcaaagt tgccgtcctg cagcagcgtg
     4381 agcgtgactt cgccggtgcc gtagcggcgg gcgttctcca gcaggttgcg caccacgcgg
     4441 cgcaacagct tggctacgcc gggcacgacc agcgaggctt gtgttgtctc caatacagcc
     4501 tggtggcggg cgcactcttc ggcagacagt ccgaccaggt cgaccggttc cacggtgccc
     4561 agatccgcct ctttcgcgtc gagccggctg gccagcagaa tctcttcgat caactggtcg
     4621 agttcagcga tgttgcgggc aatttcgtcg cgggcgctgg agctggctgg gcccatgagc
     4681 tccaggccca tgcggatgcg agtcagcggt gagcggagtt cgtgggacgc attggccagc
     4741 agggactttt gcgaggccag cagggcgtcg cgcgacttga gcagggtttc cacccgctgc
     4801 gcagcatggt tgaagcgggt ggccaggaag cccacctcgt cgtcgccacc ttcgggaacg
     4861 cgggcactca ggttgccatc gccccattgc tccacgccgc gctgcagggc ttcgagcctg
     4921 cgcgtgagcc gtcgcacgat ggggtaggtg gccagcgcca ccgctatggc gaccaggccc
     4981 agcgtccaga agaagccaaa cggtgcgcgc caggtggagg tcggtggacg gggcaggtgc
     5041 atatgcacgg tctggccgtc ctgcatctgc accagaaatt ccgggccccg gccaaagcgg
     5101 ctcgggggtt cgtccgcatc gtcgtctgca ggcgaagatg ctgcgcggga cggcggggcc
     5161 gtgcggggtg cctctgcgtc cggacggcgg cccaggcgca tgcggcctgc gccaatcacg
     5221 tctccttgcg cattgcgcac caccacttcg cgcaaaggcg gctcggtggt ggcgcgccag
     5281 gcccagccca ccagcagggt gagcacggcc acggccagca cgacggccag ccagatgcgc
     5341 acatagagtt tttgcaggat cgcagacatg gcggcgcgcg ctcagtcctg ctggcgcgca
     5401 aacacatagc ccacgccgcg caccgtcaga atgcgcttgg ggtctttggc gtctgcctca
     5461 atcgccgcac ggatgcggcc catgtgcacg tcgatcgagc ggtcaaaggc ttccagttcg
     5521 cgcccgcgca cggcttccat gatctggtcc cgtgtcagga cgcggccggc ccgctcggcc
     5581 agtgccacca gcagatcaaa ctggtaggag gtgagttcac acagcgcacc ctggacgctc
     5641 accgtgcgcg catcgcggtc gatctccagc gagccgaacc gcagggtctg ggtggtggct
     5701 gcgggagcct ccactttgcg acgcaaaacc gcgcggatgc gggccagcag ttcacgcggc
     5761 tcgaagggct tgggcaggta gtcgtcagcg cccatttcca ggccaatgat gcggtccatg
     5821 gggtcgccct tggcggtcag catgaggatg ggcgtctgcg ccatggccgt gggcagcgag
     5881 cggatgcgtc ggcacacttc caggccgtcg atatcgggca gcatcaggtc cagcaccacc
     5941 aggtccggtg gctgtccatg gcctggctgc gcaagttggt ccaggccact caggccgtcg
     6001 gcggcatggc tgacggtgaa tccgttttgc gtcaggtact ccgccaccat ggcagccagc
     6061 cgggcgtcgt cctcgatcat gagcagatgt tggtgcatgg ccgcagtcta gcgccgggcc
     6121 agtccgcgcc tatggaggct ggttgaagct gcggtaaagt ttggtaaata ttgctataaa
     6181 aacaatagct agttgcgcaa tatctacggg ggctagaggc ctatttgatg cgtgaagtca
     6241 gccagaccag cagcagcacc ggcgcaccca gcagggccgt ggaggtaaag aagctgccgt
     6301 agccgaaggc atccacatac atgccggaat agccggcgac aaacttgggc agcagcagca
     6361 tcatcgagct gaacagtgcg tactgtgtgg ccgaataatt cacattggtc aggctcgaca
     6421 ggtaggcaat gaacgccgcc gaggcgatgc cgctggccag gttgtccgca gagatcacga
     6481 agatcagcgc atgcaggtcg tggccctggc cggatagcca ggcgaacagc aggttggtgc
     6541 ccgcgctcag gatggcgccc agcatcatga cccgcatcac ccccatgcgc aaagccagtg
     6601 cgccgcccag aaaggcgccc accagcgtca tgaccacgcc atagattttg gtgacggcgg
     6661 cgacttcgtc cttggtgaag cccatgtcca cataaaacgg attggccatg atgcccatga
     6721 ccacatcgct gatgcggtag gtggcaatca gggccaggat cagcaccgcg tgccagcggt
     6781 agcgcgtgat gaactcggca aaaggctcga ccagggcgcc gcgcagccat tccatggcgt
     6841 tgcgggcctg gggcagcgcg cgcgccaccg gttcgcgcga gaacagcacc gtcagcacac
     6901 cgggcagcat gctcgccgcc atcaccagat acgcggtttg ccaggcaccc tgctggtaca
     6961 gcgcagaatt gggcacctcg gagcgcgcgg caatccacaa ggcgccggcg ccggaccaga
     7021 tcatggccag ccggtaaccc gtctggtagg tggcggccag cgcagcctgg tgctgggtgt
     7081 cggcagattc aatgcgaaag gcgtccagcg caatgtcctg cgtggcggag ccaaaggcca
     7141 ccagaacggc gcaccacacg accggagcaa gcgccagctt ggggtcggtc gtggccatca
     7201 ccaccagcgc ggccatgatg accgcctgcg atgccagcag ccagctgcgc cggcggccca
     7261 gcaggcgggt gagtaccgga atgggcatgc ggtccaccag cggcgaccac acccatttga
     7321 agccgtaggc gagccccacc cagctcagaa atccgatggt ggtgcggtca atgcccgcct
     7381 cgcgcaacca gaagctcagc gtgccaaaca ccagcagcaa cggcagacca gccgaaaacc
     7441 ccagcgaaag catgcgcagc gaggctggct cggcatacac cttcaaggtc tgcgcccagg
     7501 ttattggtgt ggttgtgggg gcgggactgg gcgtgggggc agacatcggg gaataatacc
     7561 gtggcatgaa caccctcccc ctgaagttat cgctcgcact ggccctggcc ttcccctgga
     7621 atctggcggc ggtgcacgcg cgcaatggcg tggaagttgg cggcaactcg gcctttacca
     7681 acctggtgcc ggccgagagt gtggagcgct ccgcagccca ggaatatggg caaatgctgc
     7741 agcaggccgc cagcaagaat gcgctgggcg gcaaggacca tccgcaggtc aaacgcctgc
     7801 gggccatcgc gcagcgcatc attccccatg cgatggagtg gaacccgcgt gcgcgcgagt
     7861 ggaagtggga agtcaacctg atgggttcca gccagatcaa tgcgttctgc atgcctggcg
     7921 gcaagattgc gttctacagc ggcattctcg acaagctcaa gctcaccgac gatgaagtgg
     7981 ccatggtgat gggccatgaa attgcccacg cgctgcgcga gcatgcgcgc gaacgcatgg
     8041 gcaagaatgc ggcgaccggc attggcgcca atgtattgag ccagctgctg gggctggggc
     8101 aggtagggca aacggtgacg cagtacggcg cccaattgct gacgctgcag ttcagccggg
     8161 aagatgagtc cgaagcggac ctggtgggga tggagctggg cgcgcgcgcc gggtttgacc
     8221 cgcgcgctgg tatcactctg tggcagaaga tgagtgctgc agccaagggc gcgccaccgc
     8281 agtggctgtc cacccacccg tcgggcagta cgcgcattgc tgaaatggaa gcgaacttgc
     8341 cgcgcgtgat gccgctgtac cagaaggcca gggccgcacg ctgatactgc gctggccgac
     8401 gaaggtcagt gggcgccgct ggcctttttg agaatggcgt acagctggtc cttgaggtgc
     8461 agcttttctt ttttcagatt ttcgatctcg tccggcgtgc cgggttcgac gtggttttcc
     8521 ttgttcttga tcttctggtc cagcgcgttg tgctggtcga acaggcgcgt gaaatggtgg
     8581 tcagtggtct tgagctgggt gatcagttca cggtattcag gaaacatgca aactcctctg
     8641 tagggggtag agtgaaaatc agtgcgcctt gacgcacagt acgggacagt cgaccgacat
     8701 caaaatctcc tgggcgacgc tgcccaggat cagcttgccg acggcggagc gcttgcgcaa
     8761 accgatgacg agcacatagg gctgcagtgc agtcaccagg acctcaatct cctcgatggc
     8821 actcttgccg cgaccgaact gcttgaactc cgactgcacg ccggccgcct tgagctgggc
     8881 ctcaatgcgt tcaacgtcct gcacgctggc cattgcccct tcgtctttgg cgccacccgt
     8941 ggtcgcgttg accaccagca ggcgctcatt gcggcgcttg gctatttcaa tgcccttgtc
     9001 cagggcggcc tggccttcgg ggcggggaac ataggctaca agaatcgtca tggggtgtgt
     9061 cgcttgcgcg agtgggtgtt gatgtttctg gaatcttagc ggcgaaactg ccgtgcattg
     9121 cgctcaggcg ttgaaatcgt aggcgatggt aatgggcgca tggtccgaga acttctcacc
     9181 cttgtagatg tccgctgtct tggcgccggc cgccagtgcg ggcgtggcca agtggtagtc
     9241 cagccgccac cccacgttgt tggcgtaggc ctggccacgg ttgctccacc atgtatagca
     9301 ggtgtcggtg gtgtcgggtt ccagctggcg gtacacgtcg acgatgcccc cttcgctggt
     9361 caggcgtgtc atccatgcgc gctcttctgg caggaagccg ctgtttttct ggttgctgcg
     9421 ccagttcttc aagtctgctt cccggtgggc gatgttgacg tcgccgcaca ggatgaagtc
     9481 acgttctgct ttgagtgcct gcagatgcgg gtacatcttg tcgaggaagc ggaatttggc
     9541 ctgttgccgg tcttcgcccg atgacccgct gggaaagtag caactgatga gggagtgctt
     9601 gcgctttggc gtgtcaaagc gcagttccac ccagcgacct tcggcatcga actcgtcgcc
     9661 gtcgaagcca acgatgacgt cgctgggttc cagtcgggtg taggcgccca cgccggagta
     9721 gcccttcttc tgcgcaaagt ggaagtagcc tttcaaaccc gccagctctt cgaatttgcc
     9781 ctccacatcc ggtgcctggg ctttgacttc ctgcacacaa atacaatcgg gctgtgcctt
     9841 ttcgagccag gcgacgagcc ccttgctggt ggcggagcgg atgccattga ggttcaggct
     9901 ggttaattta aacaaggaat tccccatgtc agttcaagac cccgtacagg acccacaaca
     9961 cgatgttctg gcccaggagt ttgtggagtt tgcggtgcaa tgcggggtgc tgcgctttgg
    10021 cgagttcaag accaaggcg
//
LOCUS       DAOSJM010000038         9879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6631_length_9879_cov_5.033591, whole genome shotgun sequence.
ACCESSION   DAOSJM010000038 DAOSJM010000000
VERSION     DAOSJM010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6631_length_9879_cov_5.033591"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /gene="dnaN"
                     /locus_tag="PKX82_02395"
     CDS             complement(<1..715)
                     /gene="dnaN"
                     /locus_tag="PKX82_02395"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOF51126.1"
                     /translation="MIVLKATQDKVLSVLQSVAGIVERRHTLPILANVLLRKTGTSIQ
                     LTTSDLEIQIRTTADLGGDTGNFTTTIGARKLIDILRTMPSDQTVSLESSQNKIILKG
                     GKSKFTLQTLQAEDFPLVQESANFGPVFSVPQKTLKDLLSQVSFAMAVHDIRYYLNGI
                     LFVAEGKQLSLVATDGHRLAFASATLDVDVPKQEVILPRKTVIELQRLLSDAEGAIEM
                     QFANNQAKFSFGGMEFVTKL"
     gene            complement(840..2252)
                     /gene="dnaA"
                     /locus_tag="PKX82_02400"
     CDS             complement(840..2252)
                     /gene="dnaA"
                     /locus_tag="PKX82_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOF51127.1"
                     /translation="MTEGHPSRQAESPALDIGQSLWQSCIDQLAQELPEQQFNTWIKP
                     LNAQVQEDFSRVTIYVANRFKLDWVRAQYSTRIASMLEKLYGQPVQVELAITPRETIV
                     RTNAVAQKSDPAAVEEVAEIGEDRNLGGPKNRLNSMLTFETLVEGTANRMARAAAMHV
                     ATMPGHLYNPLFIYGGVGLGKTHLMHAVGNRLLADKPNSKVLYIHAEQFVSDVVKAYQ
                     RKTFDEFKERYHSLDLLLIDDVQFFANKDRTQEEFFNAFEALLAKKSHIVMTSDTYPK
                     GLTDIHERLVSRFDSGLTVAIEPPELEMRVAILINKARAEGSEMPEEVAFFVAKNVRS
                     NVRELEGALRKILAYSRFNQKEISIALAREALRDLLSIQNRQISVENIQKTVADYYKI
                     KVADMYSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKIGGE
                     RQQMTELNQQLHVLEQTLKG"
     gene            2475..2609
                     /gene="rpmH"
                     /locus_tag="PKX82_02405"
     CDS             2475..2609
                     /gene="rpmH"
                     /locus_tag="PKX82_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOF51128.1"
                     /translation="MKRTYQPSKIRRARTHGFLVRMKTRGGRAVINARRAKGRKRLAV
                     "
     gene            2658..3062
                     /locus_tag="PKX82_02410"
     CDS             2658..3062
                     /locus_tag="PKX82_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOF51129.1"
                     /translation="MYRLKTRPQFQAVLAGRTLARTPHFVLHAAVAREGAALQMEAEA
                     LFPDAGVGLGAMVPKRWAKRAVTRNTIKRQIYTVSAEFESAMQPVAHLVRLRSAFDRA
                     QFISATSDVLKAAVRAELHALFSAVAGRGASS"
     gene            3059..3331
                     /gene="yidD"
                     /locus_tag="PKX82_02415"
     CDS             3059..3331
                     /gene="yidD"
                     /locus_tag="PKX82_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOF51130.1"
                     /translation="MMQALLIGLVKGYRMFLSPWLGSSCRFEPTCSQYSLQALQNHGA
                     AAGSYLTITRLARCHPWCEGGSDPVPAEKPRLFSRLISTPTEKKPS"
     gene            3328..5046
                     /gene="yidC"
                     /locus_tag="PKX82_02420"
     CDS             3328..5046
                     /gene="yidC"
                     /locus_tag="PKX82_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOF51131.1"
                     /translation="MNDIRRTILWVIFGFSMVLLWDQWQVHNGKKATFFPSPAQQVAP
                     AAVGASGPLPAASDAGVPAAVAQAGTAMAPVAPVAPTAPKERFEVTTDVLKLTFDTEG
                     GSLVRTEFLKYSDMADKTRNFVLLDDSKDRVYMAQTGVIAGAAGGAFPTHKTPMAVTG
                     ERVLKEGANELVVKFTSPDVGGIKLVKTYTLRRGAYDATVKHELTNTSTAAIAPQLYL
                     QLVRDGNKPAGESSFYSTFTGPAVYTEAKKYQKAEFSDIKKKNVDVEKQASNGYIAMV
                     QHYFASAWILPDGIQRNLSLDAVDIGASVPDCCYRTTMIAPLEAIAPGATKVVDAKFF
                     AGPQEEKMLESLAPGLELVKDYGWLTILAKPLYWLLDKLHSFIQNWGWSIVALVLLLK
                     IAFYWLNAKAYASMAKMKAVNPKIMEMRERLKDNPQQMQQEMMRIYREEKVNPMGGCF
                     PIMVQIPVFIALYWVLLSSVEMRNAPWAMWIHDLSSPDPYYILPLFMTLTTLLQTALN
                     PAPPDPMQAKMMWFMPLAFSVMFFFFPAGLVLYWITNNILSIAQQWIINTRMGVPPQF
                     NLPKFK"
     gene            5077..6480
                     /gene="mnmE"
                     /locus_tag="PKX82_02425"
     CDS             5077..6480
                     /gene="mnmE"
                     /locus_tag="PKX82_02425"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOF51132.1"
                     /translation="MLARHHDPIAAIATAPGRGSVGIVRISGKALGPFVLHLLRRTLV
                     PREATYLPFCDAQGVPIDHGLALFFNAPHSFTGEDVLELQAHGGPVVLQLLLARCLAA
                     AAEVNVVTGATYLPGLRIAQAGEFSERAFLNDKIDLAQAEAIADLIDASTEAAARSAT
                     QSLSGVFSREIHALRDALIQLRMLVEATLDFPEEEIDFLQKADAQGQLDRLQASLAGV
                     LQKASQGALLREGITVVIAGQPNAGKSSLLNALAGAELAIVTPVPGTTRDKVQQTIQI
                     EGVPVHVIDTAGLRESEDAVEKIGIARAWEAIGEADAVLFLHDLTRLSVTKYIAADAD
                     IAREITQKAPAKVPVIDVWNKADVATAPPQGGLVLSAKTGRGLDALRQALLQAAGWQA
                     TAGGVFIARARHIEALRKVEMHLQYASGHLAARAQSLDLLAEELRLGQNALSEITGEF
                     TSDDLLGVIFSRFCIGK"
     gene            6524..7267
                     /locus_tag="PKX82_02430"
     CDS             6524..7267
                     /locus_tag="PKX82_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51133.1"
                     /translation="MSFFDWLSRKNPAPVSDSGVSSGLGQTDATQPLFSNARPRPKAA
                     VAATHGASSSRRAERLERREQLYTVVRECMTQAGVLSSKYKFKVLSLDSKGRDYLAMI
                     DLPRENAVEPERLTDIELLIARNAKNLHGILVTAVYWRLVEQVASNTQTIRTVTPASR
                     PVVPEPLPAKVAVSVEPAVPRFEPLQDDEVAAFKKALASVPAAKVTPPPGEIIRSGRR
                     NPAPMPHFADTELDDSHSPLSGTQYGDLN"
     gene            complement(7274..7828)
                     /locus_tag="PKX82_02435"
     CDS             complement(7274..7828)
                     /locus_tag="PKX82_02435"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOF51134.1"
                     /translation="MHSFSVNQYLRDHIRTVPDWPAPGVQFRDITPLLQDAKVFRVLI
                     DAFVHRYMDPDMRPDVVAGLDARGFILGAVVAYELNVGFVPIRKKGKLPFTTVEETYE
                     LEYGSATVELHTDAVKSGDRVLLIDDLIATGGTMMAGKKLLEKLGAQVMEGAAIVDLP
                     ELGGSQRLRDGGLALYTLVDFAGH"
     gene            complement(7891..9876)
                     /locus_tag="PKX82_02440"
     CDS             complement(7891..9876)
                     /locus_tag="PKX82_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HOF51135.1"
                     /translation="MSALEITLLYLLAAVLGVVVCRSFKLPPMLGYLAVGVVIGPNAL
                     ALSQNADGVRHLGEFGVVFLMFVIGLEFNLPKLRAMRKHVFGLGLAQVVVTMLGATMV
                     SIGLAAALPQLWGMGWQTALALSCALAMSSTAIVVKLMVERIELESEHGKRVMGVLLF
                     QDLAVVPLLVLIPALGASGENLVMALSVAAIKATVLVTVLLVGGQHLMRRWLTIVARR
                     KSEELFVLNLLFITLGLAWLTELAGLSLALGAFIAGMLISETEYKHQVETDIRPFHDV
                     LLGLFFISIGMLLDWRLVLERWPLVLLLLVVPTFLKAVVVTALARILGTSTGVALRTG
                     LYLAQAGEFGFVLLTLAQQNALVPPELLNPILASMVLSMLVTPFIVMNSNRIVMKLVA
                     NEWLQQSLQMTSIARKSINTNRHVIICGYGRCGQNLARMLERENIPYMALDLDPDRVR
                     QAAAAGDSVVFGDAARLQALMAAGLARASAVVITYLDEGGALKVLGNTRAHAPQVPVI
                     VRTQDDHSLEKLQAAGATEVVPEALEGSLMLASHALALVGVPMRRVIRIVQEQRDARY
                     NLLRGFFHGSDDNSVDELQHERLATVSLPMGARASGKSVGYFALHAMGVRVVSLRRSS
                     GKTVEARDATPLEDGDTLVLSGQSETLALAESRLLKG"
BASE COUNT         2013 a         2876 c         2849 g         2141 t
ORIGIN      
        1 ccagcttggt cacgaactcc atgccgccga agctgaactt ggcctggttg ttggcaaact
       61 gcatctcgat ggcgccttcg gcgtcgctca aaagacgctg caactcgatc acggtcttgc
      121 gtggcaggat gacttcctgc ttgggtacgt ccacatccag cgtggccgac gcaaacgcca
      181 ggcggtgacc atcggtggcc accaggctga gctgtttgcc ttcggcaaca aacagaatgc
      241 cgttgaggta atagcggatg tcatgcaccg ccatggcaaa agaaacctgg ctcagcagat
      301 ccttcagggt cttttgcggc acgctgaaca cagggccaaa gttggccgat tcctgcacca
      361 gggggaaatc ttcggcctgc agggtttgca gggtgaactt gctcttgccg cccttgagga
      421 tgatcttgtt ctggctggat tccagggaca cggtctggtc ggaaggcatg gtgcgcagaa
      481 tgtcgatgag cttgcgcgcg ccaatggtgg tggtgaagtt gccggtgtcg ccacccagat
      541 cggcggtggt gcggatctgg atttccaggt cgctggtggt gagctggata gaggtgccgg
      601 tcttgcgcaa cagcacgttg gccagaatcg gcagcgtgtg gcggcgctcc acaatgcctg
      661 caacggactg cagaaccgag agcactttgt cttgcgttgc cttcaatacg atcatgtcaa
      721 cctctattaa tacggtgtct agtgcgctta gtaagcgctt gccaatgcag ttttgaccaa
      781 attcgccatt ttcccagttt tatggggcat ttctggcggt ttccggcttg tttttgcaat
      841 catcccttga gggtttgctc cagcacatgg agttgctggt tcaactcggt catttgctgg
      901 cgctcaccgc cgatctttcg cacggcgtgc agtacggtgg tgtggtcccg tccgccgaac
      961 aattctccga tttccggaag gcttttctgt gtcagttctt tcgccaggta catggcgatc
     1021 tggcgcggcc tggcaatgct ggctgggcgt ttcttggaat acatgtccgc gaccttgatc
     1081 ttgtaatagt cggccacggt tttctggatg ttttccacag aaatctggcg gttctggatg
     1141 gacagcaggt cgcgcagcgc ttcacgcgcc agcgcaatcg agatttcttt ctggttgaag
     1201 cgcgaatagg ccaggatctt gcgcagcgcg ccttcaagct cgcgcacgtt ggagcgtacg
     1261 ttcttggcga cgaaaaatgc cacttcttcc ggcatttccg agccttcggc gcgcgccttg
     1321 ttgatcagga tcgccacccg catttccagc tcgggcggtt caatggctac ggtcaggccc
     1381 gaatcaaaac gcgacaccag acgttcgtgg atgtcggtca ggcccttggg gtaagtgtcg
     1441 ctggtcatca cgatgtgcga ctttttggcc agtagcgcct caaaggcgtt aaagaactct
     1501 tcctgggtgc ggtctttgtt ggcaaagaac tgcacatcgt caatcaggag caaatccagc
     1561 gagtggtagc gctccttgaa ttcatcgaag gttttgcgct ggtacgcttt aaccacatcc
     1621 gaaacaaact gttctgcatg gatgtagaga actttgctgt tgggcttgtc cgccagcagg
     1681 cggttaccca cagcgtgcat caggtgggtc ttgcccagac cgacgccgcc atagatgaac
     1741 aaggggttgt aaagatgccc cggcatggtg gccacatgca tggcggctgc gcgggccatc
     1801 cggttggcag ttccttccac cagcgtctcg aatgtgagca tgctgttgag gcggttcttg
     1861 ggtccaccca agttgcggtc ttcaccaatc tcagcgactt cttccactgc tgccggatct
     1921 gatttttgtg ccactgcgtt agttctgaca atggtttcac gcggagtgat cgctaactca
     1981 acctgaaccg gctgcccata cagcttctcc agcatcgagg caatccgggt gctgtactgg
     2041 gcgcgtaccc agtcgagctt gaagcggttg gccacataga tggtgaccct ggaaaagtct
     2101 tcctgaacct gcgcattgag cggtttgatc caggtgttga actgctgttc gggtaactct
     2161 tgcgccagct gatcgataca gctttgccac aggctttggc caatatcgag tgcgggtgac
     2221 tcggcttgtc tggatgggtg tccctcggtc attcttcttg ttgtcttctg tggatagtta
     2281 tggtgtgcag cgcgtcgcat tgtaatttat caaaacctta tccacagaca gggtggtaat
     2341 tacccgagtt agtgcgttac acgccttttc ttgcgtaagg tgttgccggc attcgataaa
     2401 tttgcgataa tcttgggttt ccctgatcgt cttcggggta acttgactcc aatagtgact
     2461 ccaagagatt cgatatgaaa cgtacttacc aaccttccaa aatccgtcgc gcccgtaccc
     2521 acggcttcct ggtgcgcatg aagacacgcg gtggccgcgc tgtcatcaat gcccgccgcg
     2581 ccaagggccg caagcgtctg gctgtctaat tccagtctct gggttgtccc agggatgctg
     2641 tgcgcgggtt gaccaatttg tatcggctta agacccgccc acagttccag gcagttctgg
     2701 ctggcaggac gctggcgcgc accccccact ttgtattgca tgccgctgtg gcccgtgaag
     2761 gtgccgcgct gcagatggag gcagaagccc tgtttcccga tgctggtgtt ggattgggtg
     2821 ccatggttcc caagcgttgg gccaagcgtg ccgtgacccg taacaccatc aaacgccaga
     2881 tctataccgt gagcgccgaa ttcgagtccg ccatgcagcc ggtcgcgcat ctggtgcgac
     2941 tgcgttctgc gtttgatcgg gcccaattca tcagcgccac ttccgatgtg ctcaaggcag
     3001 ctgtgcgcgc cgaacttcat gcgctgttct ctgcagtcgc tgggcgtgga gcatcgtcat
     3061 gatgcaggca ttactcatcg gtctggtgaa aggctaccgc atgttcctga gcccctggct
     3121 gggctcgtcg tgcaggtttg agcctacgtg ctctcagtat tccctgcagg cgctccagaa
     3181 ccatggtgcg gcagcaggca gctatctcac tatcacccgc ttggcccgtt gccacccctg
     3241 gtgcgaaggg ggtagtgacc ccgtccccgc ggaaaaaccc cggttgttta gccggctgat
     3301 ctcaaccccc accgaaaaga aaccctcatg aacgatattc gccgcaccat cctgtgggtg
     3361 atctttggct tttccatggt gttgttgtgg gaccagtggc aggtccacaa cgggaagaaa
     3421 gcgactttct tccccagccc agcccagcag gttgctcccg ccgccgttgg cgcgtctggc
     3481 cccctgcctg ccgcaagtga tgccggtgta cctgctgctg tggcacaggc tggcacagcc
     3541 atggcacctg tggctcccgt ggcacctaca gcacccaaag agcgctttga agtcaccacc
     3601 gatgtgctta agctgacctt cgataccgag ggtggttcgc tggtgcgtac cgagtttctc
     3661 aagtactccg acatggcgga caagacccgc aactttgtgc tgctcgatga cagcaaggat
     3721 cgcgtgtaca tggcgcagac cggtgtgatt gccggcgcag ccggtggcgc gttcccgaca
     3781 cacaagacgc ctatggcggt tacgggtgag cgtgtcctca aggaaggtgc gaacgagctg
     3841 gtggtcaagt tcacatcacc ggacgtgggt ggcatcaagc tggtcaaaac ctacaccctg
     3901 cgccgcggcg cctatgacgc gaccgtcaag cacgagttga ccaatacgtc gaccgcagcc
     3961 attgcgccgc agctgtacct gcagctggtg cgtgacggaa acaagcctgc gggcgagtcg
     4021 tccttctact ccacgttcac cggtcctgcg gtgtacaccg aagccaaaaa gtaccagaag
     4081 gcagagtttt ccgacatcaa gaaaaagaat gtggatgtgg agaagcaggc cagcaatggc
     4141 tacatcgcca tggtgcagca ctactttgca agtgcctgga ttctgcccga tggcatacag
     4201 cgcaacctgt cgttggacgc tgtcgacatt ggcgcttcgg tgccggattg ctgctaccgt
     4261 accaccatga ttgcaccgct tgaagccatt gctccaggcg ccaccaaggt ggtggatgcc
     4321 aagttcttcg caggcccgca ggaagagaag atgctcgagt cgctggctcc cggtctggag
     4381 ctggtcaagg actatggctg gctgacgatt ctggccaagc ccctgtactg gctgctggat
     4441 aaattgcaca gcttcattca gaactggggc tggtcgatcg tggcgctggt gctgctgctc
     4501 aaaattgcgt tctactggct caacgccaag gcttacgcca gcatggccaa gatgaaggct
     4561 gtgaatccca agatcatgga aatgcgcgag cgtctcaagg acaacccgca gcagatgcag
     4621 caggagatga tgcgcatcta ccgtgaggag aaggtcaacc cgatgggcgg ctgcttcccc
     4681 atcatggtcc agattccggt gttcattgcg ctgtactggg tgctgttgtc cagcgtggag
     4741 atgcgcaatg cgccctgggc gatgtggatt cacgatctgt cttcgcccga tccgtactac
     4801 atcctgccgc tgttcatgac attgaccacg ctgctgcaga ctgcgttgaa ccctgctcct
     4861 ccagatccga tgcaggccaa gatgatgtgg ttcatgccgc tggccttcag tgtgatgttc
     4921 ttcttcttcc ccgccggttt ggtgctgtac tggattacca acaacatcct gtcgattgca
     4981 cagcagtgga tcatcaacac ccgcatgggt gtgccaccgc agttcaatct gccgaagttc
     5041 aagtaaccta ccgaggcgcc atgcgcctcg ttctttatgc tcgcgcgcca ccacgatccg
     5101 attgcagcta tcgctaccgc accggggcgt ggctcggtgg ggattgtccg catcagtggc
     5161 aaggcgcttg gtccgtttgt tctgcatcta ctgcggcgca cactggttcc gcgcgaggca
     5221 acctaccttc cattttgtga tgcgcagggc gtgcccattg accacgggtt ggcgttgttc
     5281 ttcaacgcac cccactcatt caccggcgaa gacgtgctcg aactgcaggc ccatggtggc
     5341 ccggtggttc ttcagttgct gctggcgcgt tgcctggcgg cggcggcgga agttaatgtc
     5401 gtcacaggcg ctacgtatct ccccggattg cgcattgcgc aggcggggga gttcagcgag
     5461 cgcgcatttc tcaacgacaa aatcgatctg gcgcaggccg aagccatcgc cgatctgatc
     5521 gatgccagta ccgaagctgc tgcacgcagt gccacccaat ccctttccgg cgtgttttcc
     5581 agagagatcc atgcactgcg ggatgcactg atccagctac gcatgctggt cgaggcgacc
     5641 ctggattttc cggaggagga aatcgatttt ctgcagaagg ccgatgcgca ggggcagctg
     5701 gaccggctgc aggcgtccct ggcaggggta ctgcagaaag cttcgcaagg tgccttgttg
     5761 cgcgagggca tcaccgtggt gattgccggg cagcccaacg caggcaagtc atcgctcctc
     5821 aatgcgctgg cgggtgcgga actggccatc gtcacgccgg tgccaggcac gacacgggac
     5881 aaggtgcagc agactattca gatagaaggc gtccctgtcc atgtcatcga taccgccggc
     5941 ttgcgcgaga gtgaggatgc ggtggaaaaa atcggcattg cccgcgcctg ggaggctatt
     6001 ggtgaagccg atgcggtgtt gtttctgcac gacttgaccc gattaagtgt taccaaatat
     6061 atagcagccg acgctgatat agcgagagaa atcacccaga aggcccccgc caaagttcct
     6121 gtcattgatg tctggaacaa ggcggatgtg gcaaccgcgc ccccgcaagg tggtctggtg
     6181 ctgtctgcaa agacgggtag gggactggat gccctgcgcc aggccttgct gcaggctgcg
     6241 ggctggcaag ctacggctgg gggcgtgttt atcgcacgtg ccaggcatat cgaggctctg
     6301 cgtaaagtgg aaatgcacct ccaatatgca tccggacatc tcgccgcacg tgcacagtca
     6361 ctggatttgc ttgccgaaga gttgcgcctg gggcagaacg ctttgagcga aattaccggc
     6421 gaatttacct ccgacgattt gctgggcgtg attttttcca gattttgcat agggaaatag
     6481 ctgccgtaca cttgccagct cgaagacgtt gaaggttcaa aacatgtcat ttttcgactg
     6541 gctctcccga aaaaacccgg cacctgtttc agactcgggt gtcagttccg ggttgggcca
     6601 aacggatgcc acacaaccgc ttttcagcaa tgcgcgcccg agacccaagg cagctgtcgc
     6661 ggcaacacac ggtgcgtcca gcagccgcag ggcggaacga ctggagcgaa gggagcagct
     6721 ctacaccgtt gtgcgcgaat gcatgacgca ggctggagtg ctttcttcaa agtacaaatt
     6781 caaggttctc tcgctggact ccaaggggcg tgactacctg gcgatgattg atctgccgcg
     6841 ggaaaacgct gtggagccag agcgcctgac ggacattgag ctgttgatcg cgcgcaacgc
     6901 caagaacttg catggaatac tggtgactgc ggtctattgg cgtctggtgg agcaagttgc
     6961 ctccaacacc cagacgattc gtaccgtgac accggcttcc cggcccgtag taccagaacc
     7021 cctacctgcc aaagttgcag tgtctgtcga gccagcggtg ccgcggtttg agcctttgca
     7081 agacgatgag gttgcagcct tcaagaaagc gctagccagc gtacccgcgg cgaaggtcac
     7141 tccaccgccg ggcgaaatta tccggtcagg ccgtcgcaac ccagcgccga tgcctcactt
     7201 tgccgatacg gaactggacg attcccactc ccctctgagt ggcacacagt acggtgatct
     7261 gaactgaacg tgttcagtgg ccggcaaaat ccaccagcgt gtacagcgca aggccaccgt
     7321 cccgcaggcg ttgggatcca cccagttctg ggagatccac aatagcggcg ccctccatga
     7381 cctgggcacc tagtttctca agcaattttt tgcccgccat catcgtgccg cccgtggcaa
     7441 tcaaatcgtc aatcagcaac acacggtcac cggacttcac tgcatccgtg tgcagttcca
     7501 ccgtagcact tccgtattcc agttcgtagg tctcctcgac ggtggtgaaa ggcaacttgc
     7561 ccttcttgcg gataggtacg aatcccacat tgagttcata ggctacgact gcgcccagga
     7621 taaatccccg tgcatccaag ccggccacca catcggggcg catgtcggga tccatgtaac
     7681 ggtgaacaaa ggcgtcgatc aggacccgga ataccttggc atcctgcagg agcggtgtga
     7741 tatccctgaa ctgaacgccc ggcgcgggcc agtcgggcac ggtacggata tggtctcgca
     7801 ggtactggtt cacgctgaaa ctgtgcatgg cgcatctctt ggaagtcaat ggggcagact
     7861 tgattgtgcc tgctgcagaa ccctgcgggc ctaacctttg agcaatcggc tctccgcaag
     7921 tgccagggtt tccgattggc cagagagtac cagagtgtca ccatcttcca gtggggtggc
     7981 atctcgtgcc tcaacggttt tgccgctgct gcgccgcaga ctcaccacac gcactcccat
     8041 cgcatgcagg gcgaagtacc ccacggattt gcctgatgct ctggctccca tgggcaggct
     8101 caccgtggcc agtcgctcat gctgcaactc gtccactgag ttgtcatctg atccatggaa
     8161 aaacccgcgt agcaggttgt agcgcgcgtc ccgctgttcc tgcacgatac ggatcacccg
     8221 acgcataggt acacccacaa gcgccagagc atggctggcc agcatgaggg agccctccag
     8281 cgcttcggga acgacttcgg tggcacccgc agcttgcagc ttttccaggc tgtggtcatc
     8341 ctgggttctc acaatgacgg gaacctgtgg cgcatgggca cgggtgttcc ccagaacctt
     8401 gagcgcgcct ccttcgtcca gataggtaat cacgacggca ctggcacgtg caagacccgc
     8461 tgccatgagg gcctgcagtc gcgctgcatc tccaaatacg acggaatccc ctgcagcagc
     8521 agcctggcgc acacggtccg ggtccaggtc cagtgccatg tagggaatgt tttccctctc
     8581 aagcatgcgt gccagatttt ggccacaccg accgtagcca caaatgatca catggcgatt
     8641 ggtgttgatg gacttgcgtg caatgctggt catctgcaac gactgttgca gccactcatt
     8701 ggcgaccagt ttcatcacaa tccggttgct gttcatcaca atgaacggcg tgacaagcat
     8761 ggacagcacc atgcttgcaa ggatcggatt gagcaattcc gggggcacca gtgcgttctg
     8821 ctgcgccaac gtcagcagta cgaatccgaa ctcccccgct tgtgccagat acaggccagt
     8881 gcgaagcgct acacccgtac tcgtgcccag aatgcgcgcc agtgcggtca ccaccaccgc
     8941 cttgagaaaa gtggggacaa ctagcagaag caggaccagt ggccagcgct cgagtaccaa
     9001 tcgccaatcc agcagcatcc cgatgctgat gaagaacaga ccgagtagaa catcgtgaaa
     9061 cggcctgata tcggtttcca cctggtgctt gtattcggtt tctgaaatca gcatacctgc
     9121 aataaaggct cccagcgcga ggctcaggcc cgccagttcc gttaaccatg ccagaccgag
     9181 ggtgatgaag agcaggttga gtacaaacaa ttcctcactt ttgcggcgcg ccacgatagt
     9241 cagccagcgt cgcatgaggt gctgacctcc gaccagcagc accgtgacca gaacagttgc
     9301 cttgatggct gcaacgctca gggccatcac gaggttctca cctgatgcac ccagagcggg
     9361 aatgagaacc agcagaggaa caacagcgag atcctgaaac agcagaacac ccatgacccg
     9421 cttgccatgc tctgactcca gctcaatgcg ctccaccatc agcttgacca caatagcggt
     9481 actgctcatg gccagtgcgc atgacagagc cagagcggtt tgccagccca tgccccacaa
     9541 ctgcggcagt gcggcagcca atccgatgga gaccatagta gcccctaaca tggtgaccac
     9601 cacctgtgcc agacccaacc cgaatacgtg cttgcgcatg gcgcgcaact tgggcaggtt
     9661 gaattccagg ccaatcacga acatcaggaa cactacgccg aactcgccga ggtgtcggac
     9721 gccatctgcg ttctgcgaca gggccaaggc gttgggaccg atgaccacgc caacggccag
     9781 ataccccagc atcggcggca atttgaacga gcggcacacg acgacaccca ggaccgcggc
     9841 cagcaggtac aaaagggtaa tttccaatgc gctcatgcc
//
LOCUS       DAOSJM010000039         9812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6681_length_9812_cov_4.167674, whole genome shotgun sequence.
ACCESSION   DAOSJM010000039 DAOSJM010000000
VERSION     DAOSJM010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6681_length_9812_cov_4.167674"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PKX82_02445"
     CDS             <1..517
                     /locus_tag="PKX82_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HOF51136.1"
                     /translation="AATELHGVKIEDTITVAGTKLQLNGAGTRYKGPFKVYVGDLYTT
                     KKVGSLEELVAAPGPKRLTMVFLREIEAGPFGKLLTRGVEDNVPRAELSKLIPGLIRM
                     GEIFTVNKVILPGDVITLDWIPGTGMVITAKGKVQGEPFKEPEFFKAIMSIWFGPVPA
                     DFKLKDAMLGK"
     gene            659..2200
                     /locus_tag="PKX82_02450"
     CDS             659..2200
                     /locus_tag="PKX82_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOF51137.1"
                     /translation="MKLRLKLPLAFAFSLALMLAAALFGIYRLNQAIGTFENDVLLHV
                     AAHKKAAEVSTRFSTAIQEWKNVLLRGKKPEDMEKYWSAHAKEMNEVNKAIQELDKLI
                     EDTSPQQTEVSRLATQMVGAQDGYRQAFETYKATAFDFAAGDQAARGKDREAATTLLA
                     LQSALAKQEAATSAYAAANAKSATQLSLLVMACVVLAAMVGAFHLSRQIVASLSSAVQ
                     VADKVARGDLTQTIEPRSRDEIGALLQSLKAMQSSLVALVSNVRQGSDSVASASAEIA
                     QGNHDLSARTEQQASSLEETAASMEELGSTVRQSADSARQANQLATNASTVATRGGEV
                     VSQVVDTMKGINESSRKISDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS
                     EVRSLAGRSAEAAKEIKSLINTSVERVEHGSSLVDQAGATMSEVVSAIRRVTDIVGEI
                     SAASGEQSAGVSQVGEAVSLIDQTTQQNAALVEQMAAAASSLKMQAQELVQTVSRFKL
                     NTAVA"
     gene            2251..3807
                     /locus_tag="PKX82_02455"
     CDS             2251..3807
                     /locus_tag="PKX82_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOF51138.1"
                     /translation="MKLRSQIVGLGLVGVLMTALVGGVGLINAARLSAAFEESIAMNL
                     ALSKSQEADMMHDAIRGDVLRSLFSAQRNDNAGLAEATKDLNEHADNFSKNIAEMQAL
                     PISDAVKTISAKTAPLVKAYIDSAAKVQSLAAKDAISAEAAIPEFQKAFKDLEVAMEQ
                     QGDAIAKDVEAYTEQTTTLASRSKILALVGVLITAALMTLAALWLAKRLSTPMSRAVV
                     VADRIAEGGLASTIVPAGSDETIQLLEAMARMQTNFGNIVRTVQTNAESVATASAEIA
                     QGNHDLSARTEQQASSLEETAASMEELGAQVKHNADNARQANQLAQSASTVAVQGGEV
                     VAQVVDTMKGIVDSSRRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS
                     EVRSLAGRSAEAAREIKNLIGTSVERVEHGTALVDKAGATMVDVVASIRRVTDIMGEI
                     SSASNEQSLGVAQVGEAVTQMDHATQQNAALVEQMAAAASSLKSQAGELVQAVAVFKL
                     GASAGVPAIR"
     gene            complement(3767..4495)
                     /gene="gph"
                     /locus_tag="PKX82_02460"
     CDS             complement(3767..4495)
                     /gene="gph"
                     /locus_tag="PKX82_02460"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592997.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOF51139.1"
                     /translation="MKQTYDIVQTLPWPLSQFDAALVDLDGTLVDTLDDFVESLQRML
                     GELPAPYCTQVVQRRQVEQMVGKGSENLVNSLLDTIDSVHGTAALPAIRALALDAYLR
                     HYREINGLYARVFKGAREGLEVFRAAGWKLACVTNKPTDYARALLRLQKLDGYFEVVL
                     GGDATPRKKPDPMPLLAACAALDTRPARTLMVGDSSNDAQAARAAGCPVLLVTYGYNH
                     GEPILQVDADAFTESLAHLRWRPA"
     gene            4546..6405
                     /locus_tag="PKX82_02465"
     CDS             4546..6405
                     /locus_tag="PKX82_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51140.1"
                     /translation="MTVEDEAAIAVREGLLLGEQGIRTFRLVLLVVVVTTAALGLYSW
                     HIDGLRTTYLAVWFVSAITIGAYMLAQHHMLRSAIQLLLWGATLAIAAQCFLVAGVRT
                     PALMFMPAVCMVAAWLIGVRTATAICLVIALEIIGMLIAEQYGYTPPQVVRTSVGHGL
                     IVLPSMALALLVSMGAIRSFSQHLTRVTELTREQTRRLDELRQSEERFSALFRANPLP
                     SSTNDQEGRIIAVNDAWLALYGRQRDEVMGKTAEEVGIWTNAQDRKKIYTELSQSGRV
                     DGMPLLLQDGQGDRKPFLIYIAPVEFGGQQRFVTTLHDQSDRVAAEAAQRAVQDVLEE
                     RVAQRTAELSQTVRDLTATQEELVQAEKLASLGAMVAGISHELNTPIGNTLTVASTLH
                     GQVLELKAAVERGDLKRSTLTQFLDRLEDMSDLISRSALRSGELIKSFKQVAIDRTSE
                     RRRTFDLHDLVNDIVTSMMPGMHKTNIQVARSIPADVPCDSFPGPVGQILTNLIQNAV
                     LHAFAGRAGGNIAISATVEGTGRQAQVVLTVNDDGVGMPDHVRHRVFDPFFTTRLGQG
                     GSGLGLSISYRLATATLGGSLTVDSTPGQGSCFTLTFPQAAPYKPEESLESGF"
     gene            complement(6402..7079)
                     /gene="rpe"
                     /locus_tag="PKX82_02470"
     CDS             complement(6402..7079)
                     /gene="rpe"
                     /locus_tag="PKX82_02470"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOF51141.1"
                     /translation="MTYRIAPSILSADFARLGEEVKNVIAAGADWIHFDVMDNHYVPN
                     LTFGPMVCQALKPHAKTAAGVAVPIDVHLMISPVDALAGAFADAGADLISFHPDASGH
                     VHRSIQAIKSKGVKAGLTFNPAEPLDVLDWVIDDIDLVLIMSVNPGFGGQSFIDSALR
                     KIEDVRKRIDACGKDIRLEVDGGIKTDNIRRVADAGADTFVAGSAIFGKPDYKAVVDA
                     MRQQLAA"
     gene            7139..7540
                     /gene="apaG"
                     /locus_tag="PKX82_02475"
     CDS             7139..7540
                     /gene="apaG"
                     /locus_tag="PKX82_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOF51142.1"
                     /translation="MAKYQFTVEVVPEYLPDQSAPDEDQYVFAYTITITNTGDVTAQL
                     ISRTWNVNDANGHTDKVKGLGVVGQQPLLKPGQAFEYTSGTRLRTPTGTMHGSFFCVA
                     EDGEKFDADIPMFVLDALSGQSGTGGPRTLH"
     gene            7540..9534
                     /locus_tag="PKX82_02480"
     CDS             7540..9534
                     /locus_tag="PKX82_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HOF51143.1"
                     /translation="MATRTASGDISTLLETLDPAANLVERHLWLIALMQWIRGDNRTP
                     EAAVARVDLLLDVLDARPQTTETLQAWWRAVVETVDGSTLLSDYGFGSRNAFVSELVD
                     RLHQKLLPATPETADASELFGLVLNDAHDAQWLAALPTPTLQRLARLLSTPATGHNAT
                     LRPGLTYWHSTLLDAMTFCTSQIRATGFSTEIRLRMRNPAQDASPFHTLAADLDAVCD
                     AWMAGQDTEAAVLHFRTQLDACRHAAASVYTHLDAHGISMDLVFRLRQLRERVLRVRA
                     LLDCLFDDDSHRASARLLSHLASVSQEQRSIGALVASNSSLLAAKVAERSSETGEHYI
                     TRTRAEYRTMLHNAAGGGALTALTTAMKFGVMALGLSAFWYGFWSGVVYALSFVVIQL
                     LHFTLATKQPAMTAPAMAAKLKDIADTQALGAFVDEVTHLVRSQVAAVIGNVAVVFPV
                     AIALSVAMYFGLGRPMITEEQAHYVLHSLTLLGPSLLFAAFTGVLLFASSILAGWVEN
                     WFVLHRLDSAIRYNPRITGLLGPARADRWAHFMRHNISGLAANISLGFMLGLVPPILA
                     FLGPSLDVRHVTLSAGQLGAACASLGWDVVKMPALWWAVASVPLIGVLNVGVSFYLAF
                     RVALRAHSVTGVGRTRIQRAVLQRLRQAPLSFFWPGRDGN"
     gene            9549..>9812
                     /locus_tag="PKX82_02485"
     CDS             9549..>9812
                     /locus_tag="PKX82_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOF51144.1"
                     /translation="MGEFELIARYFKRPVRKAALGVGDDCALLQPQPGMQLAISSDML
                     VSGRHFFADVDPRTLGHKALAVNLSDLAACGATPLAFTLALALP"
BASE COUNT         1937 a         3106 c         2995 g         1774 t
ORIGIN      
        1 ggccgccacc gaactccacg gcgtgaagat cgaagacacc atcacggtcg ccggcaccaa
       61 gctgcagctc aatggcgctg gcacgcggta caagggcccg ttcaaggtgt atgtgggtga
      121 tctgtacacc accaagaagg tggggtctct ggaagagctg gtcgctgccc ctggccccaa
      181 acgattgacc atggtttttt tgcgggaaat cgaagcgggt ccctttggca agctgctcac
      241 ccgtggcgtg gaggacaacg tgcccagggc tgaactgtcc aagctgattc ccggcctgat
      301 ccgcatgggc gaaattttta cggtcaacaa agtcatcttg cccggtgatg tcatcaccct
      361 ggactggatt cccggaactg gcatggtcat taccgccaag ggcaaggtgc aaggcgagcc
      421 gttcaaggaa cccgaattct tcaaagccat catgtccatc tggttcggcc cggtccctgc
      481 agacttcaaa ctcaaagacg ccatgctggg caagtgatac ctgccggggt tttactcccc
      541 ggcaatatgc agtcatcagg aaaaacacct ctttagttgg tgacacaacc accgtctccg
      601 tataaattgc gaccgtgggt gcccagcgag gctccgcatt aacttacgga ggatggcgat
      661 gaaactgcgt ctgaagcttc ccctggcatt tgctttttcg ctggcactca tgctcgcggc
      721 agccctgttc ggcatttatc gactgaatca ggccataggc accttcgaga acgacgtgtt
      781 gctgcacgtc gcagcccaca agaaggcggc cgaagtctcc acccgcttct ccaccgccat
      841 ccaggagtgg aagaacgtcc tgctgcgcgg caagaagccc gaagacatgg aaaagtactg
      901 gagcgcccat gccaaggaga tgaacgaagt caacaaggcg atccaggagt tggacaagct
      961 cattgaagac acatccccgc aacagaccga agtgtcccga ctcgccaccc agatggtggg
     1021 cgcacaagac ggctaccggc aagcctttga aacctacaag gccactgcgt ttgactttgc
     1081 cgccggagac caggcagccc gaggcaaaga cagggaggca gccaccacgc tgctggcact
     1141 ccagagcgca ctggccaagc aggaggcagc gacctctgcc tatgcagcgg ccaatgccaa
     1201 gagtgccacg caactctccc tgctggtcat ggcatgcgtg gttctcgccg ccatggtggg
     1261 cgcgttccat ttgagccgcc agattgtggc gtctttgtcc agcgccgtgc aggtcgcgga
     1321 caaagtggcg cgtggcgacc tcacccagac catcgagccc cgcagccgcg acgagatcgg
     1381 tgccctgctc cagtcactca aggccatgca gtccagcctt gtggcattgg tatccaacgt
     1441 acgacagggt tcagacagtg tggccagcgc aagcgctgaa atcgcccagg gcaaccacga
     1501 tctgtcggcc cgcaccgagc aacaggccag ctcactggag gagacggccg ccagcatgga
     1561 agaactggga tccaccgtcc ggcagagtgc ggacagcgca cgccaggcca atcagctggc
     1621 caccaacgct tcgaccgtgg caacacgggg tggagaagtg gtctcgcagg tggtggacac
     1681 catgaaaggc atcaacgagt cctcgcgcaa aatttccgac atcatcagtg tcatcgacgg
     1741 tatcgccttt cagaccaaca tcctcgcgct caatgcggcc gtggaagctg cacgcgctgg
     1801 cgaacagggc cggggctttg ccgtggtggc cagtgaggtg cggtccctgg ccggtcggtc
     1861 tgccgaagcg gccaaggaga tcaagtcact catcaacacc agcgtggagc gtgtggagca
     1921 tggttccagc ctggtcgacc aggcgggcgc caccatgagt gaggtggtca gcgccatccg
     1981 gcgcgtgacc gatatcgtcg gagaaatcag tgccgccagt ggcgaacaaa gtgccggtgt
     2041 ttctcaggtc ggggaggcgg tgagtctgat tgaccagacc acccagcaaa atgcagcact
     2101 ggtggagcag atggcagccg cggcatccag tctgaaaatg caggcgcagg agctggtgca
     2161 gacggtgtcg cgtttcaagc tgaacaccgc cgtggcttaa cctcggtcgc gccgaagcat
     2221 gggaaacttt ctaaacatgg gaactcttac atgaaactac gtagccagat tgttggcctt
     2281 ggcctggtcg gcgtgctcat gactgcgctg gtaggtggcg tcggcctgat caatgccgcg
     2341 cggctgtcgg ccgctttcga agaatccatc gccatgaacc tggcattgag caagagccag
     2401 gaagcggaca tgatgcacga tgccattcgt ggtgatgtac tgcgcagcct cttcagcgcc
     2461 cagaggaacg acaacgcggg tttggccgag gcgaccaagg acttgaatga gcatgccgac
     2521 aatttcagca aaaatattgc cgagatgcag gcgcttccca tctcggacgc ggtcaaaacc
     2581 atctccgcaa aaacagcccc gctggtaaag gcctatatcg attcggcagc gaaggtccag
     2641 tctctggcag ccaaggatgc catatccgcc gaagccgcga taccagagtt ccagaaagcg
     2701 ttcaaggacc tggaggtggc catggagcaa caaggcgatg ccattgccaa agacgtggaa
     2761 gcctatacag agcaaaccac cacgctggcc agccgctcaa aaatcctggc tttggtgggt
     2821 gtgctgatta ccgctgcctt gatgacgctc gctgcactgt ggctggccaa acggctgtcc
     2881 actccgatgt cacgtgctgt cgttgtcgcg gaccgtattg ccgaaggggg cctggcgtcc
     2941 accattgttc ctgcgggttc tgacgaaacc atccagctgc tagaagccat ggcacgcatg
     3001 cagaccaact ttggcaacat cgtgcggact gtccagacca acgcggagag cgttgccacc
     3061 gccagtgcag aaatcgcaca gggcaaccac gacctctctg cccgtaccga acagcaggcc
     3121 agctctcttg aagaaactgc cgccagcatg gaagagctgg gcgcccaggt caaacacaat
     3181 gcagacaacg cacgtcaggc caaccagctg gcgcagagtg catccactgt ggccgtgcag
     3241 ggcggtgaag tcgtggccca ggtggtggac accatgaagg gcatcgttga ttcgagccgc
     3301 aggattgccg acatcatcag tgtcattgat ggcattgcct tccagaccaa tatcctggcc
     3361 ctcaatgcag cggtggaagc agcccgtgcc ggcgagcagg gccggggttt tgctgtcgtg
     3421 gcctcggagg tgcgcagcct ggccgggcgc tccgccgaag cggcacgcga gatcaagaac
     3481 ctgattggga ccagcgtgga acgcgtcgaa cacggtacgg cactggtaga caaggcgggg
     3541 gccacgatgg tcgatgtggt cgcatccatc cgccgcgtca cggacatcat gggtgaaatc
     3601 agctctgcca gcaacgaaca gagcctgggt gtggctcagg ttggcgaagc ggtcacacaa
     3661 atggaccatg ccacgcaaca gaacgcggcc ctggtcgagc agatggccgc cgccgccagc
     3721 agcctcaaaa gccaggcggg tgaattggtg caggccgtag cggtgttcaa gctgggcgcc
     3781 agcgcaggtg tgccagcgat tcggtgaacg cgtctgcatc gacctgcaaa atcggctccc
     3841 catggttgta gccataggtg accagcagta ccgggcaccc cgcagcgcgt gcggcctgcg
     3901 cgtcgttgct ggagtcgccc accatcaggg tccgcgcagg ccgtgtgtcc agtgctgcac
     3961 aggctgccag taaaggcatg gggtccggct ttttacgggg tgtagcgtcg ccgcccagca
     4021 ccacctcaaa gtagccatcc aatttctgca agcgaagtaa tgcacgggcg taatcggtgg
     4081 gcttgttggt cacacacgcc agcttccagc ctgctgcgcg gaagacctcc agcccttcac
     4141 gcgcgccctt aaaaacacgc gcatacagac catttatctc gcgatagtgc cgcaggtagg
     4201 catccagtgc cagagcccgg atagccggca aggcagcggt accgtgcacg ctgtcgatgg
     4261 tatccagcag gctgttcacc aggttttccg agcccttgcc caccatctgc tccacctgcc
     4321 gacgttgcac cacctgcgtg cagtaagggg caggcaactc acccagcatt cgctgcaggc
     4381 tttccacaaa atcatccagg gtgtccacca gtgtgccgtc cagatccacc aaagcggcgt
     4441 cgaactggct caacggccat ggcagggtct gcacaatgtc ataggtctgt ttcataatgg
     4501 atttttcact ggtcacgctc aaaaggtatg cacggcactg cccccatgac tgtagaggat
     4561 gaagcagcca tcgcagtgcg cgaaggcttg ctgctgggcg agcagggcat ccggactttt
     4621 cgtctggtgc tattggtcgt cgttgtgacc accgcagcgc tgggcttgta cagctggcac
     4681 atcgatggcc tgcgcaccac ctacctggcg gtctggtttg tcagcgcaat caccatcggt
     4741 gcctacatgc tggcgcaaca ccacatgctg cgcagtgcca ttcagttgct cttgtggggc
     4801 gccactctgg ccatcgcagc gcaatgcttt cttgtcgccg gagtgcgcac cccggcactg
     4861 atgttcatgc cggccgtgtg catggtggcg gcctggctca tcggggtacg caccgccaca
     4921 gcgatctgtc tggtgattgc acttgaaatc atcggaatgc tcattgccga acagtatggc
     4981 tacacaccac cccaggttgt ccgcacctcc gtaggccacg gactgatcgt cctgccctcc
     5041 atggcactcg ctttgctggt gtccatgggc gccattcgaa gcttcagcca gcacctcacc
     5101 cgcgtgaccg agttgacccg ggaacagacg cgtcggctgg atgagctacg gcagtcggaa
     5161 gagcgcttct cggcattgtt ccgcgccaac cccctgcctt caagcaccaa cgaccaggaa
     5221 ggccgcatca ttgccgtcaa cgacgcctgg ctggcgctct acggacgcca acgcgacgag
     5281 gtcatgggca aaacagcgga agaggttggc atctggacca acgcccagga ccgcaagaag
     5341 atctacacag agctgtccca atccggccgg gtggatggca tgcccctgct gctgcaggat
     5401 gggcagggcg acagaaagcc attcctgatc tacatcgctc ccgtggaatt tggcggacag
     5461 cagcggtttg tcaccacgct gcatgaccag agtgaccgag tagccgcgga agccgcccag
     5521 cgtgccgtgc aggatgtgct ggaagagcgt gtggcccagc gcaccgccga actgagccag
     5581 accgtgcggg acctcacagc gacccaggaa gaactggtgc aggcagaaaa gctggcatca
     5641 ctgggtgcca tggtggccgg catttcccac gagctcaata cgccgattgg caacacgctg
     5701 accgtggcca gcacactgca tgggcaagtg cttgaactca aggcagcggt cgaaagaggt
     5761 gatctcaaac gctccacact gacccagttt ctggaccgac tggaagacat gtcggacctg
     5821 atctcccgct cggccctgcg ctccggcgaa ctcatcaaga gtttcaagca ggtcgccatc
     5881 gaccggacct cggagcggcg ccgcactttt gacctccatg acctggtcaa cgacatcgtc
     5941 acctcgatga tgccgggcat gcacaaaacg aacattcagg ttgcgcgcag catcccggcg
     6001 gatgtgccat gcgacagctt tcccggcccc gttggccaga ttctgaccaa cctgatccag
     6061 aacgccgtgt tgcatgcatt tgcaggccgc gcgggcggca acatcgccat ctccgccacg
     6121 gtcgagggca ctgggcggca agcacaagtg gtgctgacgg tcaacgacga tggcgtgggt
     6181 atgccggatc acgtccgcca ccgggtgttt gaccccttct tcaccacgcg cctgggccag
     6241 ggaggctcgg gtctgggcct gtccatctcg taccggctgg cgaccgccac actcggcggc
     6301 agcctgaccg tagactcaac accaggccag ggcagctgct ttacgctgac cttcccccag
     6361 gcagcaccct acaagcccga agaatcgctg gaatccggct tttaagccgc gagctgctgg
     6421 cgcatggcat ccaccacagc cttgtaatcc ggcttgccaa agatggcgct gccggccaca
     6481 aacgtgtcgg cacccgcatc ggccacgcgg cggatgttgt cggtcttgat gccgccgtcc
     6541 acctccagac gaatgtcctt gccgcacgcg tcaatgcgct tgcgcacgtc ttcgatcttg
     6601 cgcagggcgg aatcgataaa gctctggccg ccaaaaccgg ggttgacgct catgatcagc
     6661 accaggtcga tgtcgtcaat cacccagtcc agcacatcca gtggttcggc ggggttgaag
     6721 gtcagtcctg ccttgacccc cttgctcttg atggcctgga tgctgcggtg gacgtggccg
     6781 gaagcatcgg ggtggaagct gatcaggtcg gcccccgcgt cggcaaaggc gccggccagc
     6841 gcatccaccg gcgagatcat caggtgcaca tcgatgggca cggccacgcc cgcagcggtt
     6901 ttagcatggg gtttgagggc ctggcagacc atgggaccga atgtgaggtt gggcacatag
     6961 tggttgtcca tcacgtcaaa gtggatccag tcggccccgg cagcgatcac gtttttgacc
     7021 tcctcgccca ggcgggcaaa gtcggcagac aggatggagg gggcaatgcg gtaggtcatg
     7081 gcaggggctt tccggtgggg gtttcagata gcgcattttc gcaggtagga tggcgcccat
     7141 ggccaagtac caattcacgg tggaagtggt tcccgaatac ctccccgacc aatccgcacc
     7201 cgacgaggac cagtacgtct tcgcctacac catcaccatc accaatacgg gtgatgtgac
     7261 agcgcagctg atctcccgca cctggaacgt gaacgacgcc aatggccaca ccgacaaggt
     7321 caagggcctg ggcgtggtgg gtcagcagcc cctgctcaaa cctggccagg cgttcgagta
     7381 caccagtggt acacgtctgc gcacccccac cggcaccatg cacggcagtt tcttctgcgt
     7441 ggccgaggat ggtgaaaaat tcgatgccga cattcccatg ttcgtactgg acgcactcag
     7501 cggccagtcc ggcacgggtg gcccgcgcac gctgcactga tggccacacg cacggcatcc
     7561 ggcgatattt ccaccctgct ggagacgctc gatcccgcag cgaatctggt ggagcggcac
     7621 ctctggctga tcgccctgat gcaatggata cgtggcgaca accgcacccc cgaagccgcc
     7681 gtggcccgcg tggacctgct gctggacgtg ctggatgccc gcccgcaaac caccgaaacg
     7741 ctgcaggcat ggtggcgcgc cgtggtggag acggtcgacg gcagcaccct gttgtccgac
     7801 tacggttttg gctcgcgcaa tgcgtttgtg agcgagctgg tcgaccgcct gcaccaaaaa
     7861 ctgctgcctg ccacgccgga gaccgccgat gcctcggaat tgttcggact ggtgctgaac
     7921 gacgcacacg atgcccaatg gctggcggcc ctgcctacac ccacactgca gcgcctggcc
     7981 cgactcctga gcacgccagc tacaggacac aacgccacac tgcgccccgg cctgacttac
     8041 tggcacagca ccctgctgga cgccatgacg ttttgcacca gccagattcg ggccaccggt
     8101 ttttccacag aaatacgcct gcgcatgcgc aaccccgcgc aggatgccag ccccttccac
     8161 accctggcgg ccgacctgga cgcggtgtgc gatgcctgga tggctggcca ggacaccgag
     8221 gcagccgtgc tgcactttcg tacccagctc gatgcctgcc gccatgcggc cgcgtcggtg
     8281 tacacccacc tggacgcaca tggcatctcg atggatctgg tgttccgcct gcgccagctg
     8341 cgcgagcgtg tactgcgggt gcgcgccttg ctggactgcc tgttcgatga cgacagccac
     8401 cgtgccagcg cgcgcctgct ctcgcacctg gccagcgtga gtcaggagca acgcagcatt
     8461 ggcgcgctgg tggcgtccaa ctcgtcgctg ctggcggcca aagtggccga acgcagcagc
     8521 gaaaccggcg agcactacat cacccgcacc cgtgccgagt accgcaccat gctgcacaac
     8581 gcggcgggcg gcggcgcact cacggcgctg accacagcca tgaagttcgg cgtgatggca
     8641 ttggggctct cggcgttctg gtacggcttc tggtcggggg tagtctacgc actgagcttt
     8701 gtcgtcatcc agttgctgca cttcaccctg gcgaccaaac agcccgccat gacggccccg
     8761 gccatggccg ccaaactcaa ggacattgcc gacacgcagg ccctgggcgc ttttgtggac
     8821 gaggtcaccc atctggtgcg ctcgcaggtc gcggcggtca ttggcaatgt ggcagtggtg
     8881 tttccagtcg cgattgcgct gtcggtagcc atgtactttg gactgggccg gcccatgatt
     8941 accgaagagc aggcgcacta tgtactgcac tcgctcaccc tgctgggccc gtcgctgctg
     9001 tttgcggcgt tcaccggcgt actgctgttt gccagcagca ttctggcggg ctgggtggaa
     9061 aactggtttg tgctgcaccg gctggactcg gccatccgct acaacccgcg catcacgggg
     9121 ctgctggggc cagcgcgggc cgaccgctgg gcccacttca tgcgccacaa catctcgggc
     9181 ctggccgcca atatctcgct gggcttcatg ctcggtctgg tgcctcccat cctcgcgttt
     9241 ctgggcccca gcctggatgt gcgccacgtc accctgtctg ccggacaact gggtgcggcc
     9301 tgcgccagcc tgggctggga cgtggtgaag atgcccgccc tgtggtgggc cgtggccagc
     9361 gttcccctga ttggtgttct caatgtgggc gtgagttttt acctggcttt tcgggtcgcg
     9421 ctgcgggcgc acagcgtcac cggcgtgggc cgcacgcgca tccagcgcgc ggtacttcag
     9481 cgcctgcgcc aggcaccgct gagctttttc tggcccggcc gcgacgggaa ctgaacgcca
     9541 tggcggaaat gggcgaattc gaactgattg cgcgctactt caagcgcccg gtgcgcaagg
     9601 ccgcgctggg tgtgggcgac gactgtgccc tgctgcagcc ccagcccggc atgcagctgg
     9661 ccatctccag cgacatgctg gtcagcggcc gccatttctt tgccgatgtg gacccgcgca
     9721 cgctgggcca caaggcgctg gcggtcaacc tcagtgacct ggcggcctgc ggtgccaccc
     9781 cgctggcctt caccctggcg ctggccttgc cg
//
LOCUS       DAOSJM010000040         9745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6735_length_9745_cov_4.359443, whole genome shotgun sequence.
ACCESSION   DAOSJM010000040 DAOSJM010000000
VERSION     DAOSJM010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9745
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6735_length_9745_cov_4.359443"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..632)
                     /gene="gspG"
                     /locus_tag="PKX82_02490"
     CDS             complement(168..632)
                     /gene="gspG"
                     /locus_tag="PKX82_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOF51145.1"
                     /translation="MSQRITFPTLPHLRNVRRGLATGFTLIELMVVLVIIGVLAALIV
                     PNVLDRADDARATAARTDVNNLMQALKLYRLDNQRYPAAAQGLQALVAKPTTAPVPGN
                     WKPYLDKLPNDPWGNAYQYLNPGIRGEIDVLSFGADGQAGGEGKNADIGSWN"
     gene            678..1112
                     /locus_tag="PKX82_02495"
     CDS             678..1112
                     /locus_tag="PKX82_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOF51146.1"
                     /translation="MFGVKAATFALSALAAASLAYWGLKVFGTPAPGAVAPVASVVGM
                     AAIDPQAVVRALGGGEVAVPVAAVAPSASSRFSLKGVVADTSNGGAALISVDGKPARP
                     FQVGDLVDGRLVVQSVTGRKASLAAGLSAPVEVVLELPPLPQ"
     gene            1112..1855
                     /locus_tag="PKX82_02500"
     CDS             1112..1855
                     /locus_tag="PKX82_02500"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOF51147.1"
                     /translation="MRSAPPARNPALDALRGFAVLWMTAYHFSFDLNHFGLIRQDFYT
                     DPVWTVQRTCILSLFLLCAGGGQAMAVASGQSWRGFWRRWSQIAGCAVLVSVGSYFMF
                     PNSWIYFGVLHGMAVMLVVCRLTASWGHGLWLAGAVAVALGQWGPQLHALWPSAEVLN
                     GRGLNWLGLISRKPITEDYVPLLPWMGVMWWGMAAAQLALARGWLLPAQRRSVGARMG
                     LAFVGRWSLSWYMVHQPLLMGLLTLWRAL"
     gene            2188..2394
                     /locus_tag="PKX82_02505"
     CDS             2188..2394
                     /locus_tag="PKX82_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51148.1"
                     /translation="MGKYRIAACPEKLPCGQYAAQVSIASGRGSASTDRVMRFHDEFA
                     THDAASRFSIAQGIDWVRATTRPH"
     gene            2415..2669
                     /gene="infA"
                     /locus_tag="PKX82_02510"
     CDS             2415..2669
                     /gene="infA"
                     /locus_tag="PKX82_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOF51149.1"
                     /translation="MAKEELIEMMGVVNEVLPDTRFRVTLENGHQLIAYSAGKMRKNH
                     IRILAGDRVSLELSPYDLSKGRINFRHIEGRGPMTPRRTN"
     gene            2792..3364
                     /locus_tag="PKX82_02515"
     CDS             2792..3364
                     /locus_tag="PKX82_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51150.1"
                     /translation="MFKFSRASKAKPDSQFAETTMVTAGGSTVAAGGFQRDLIYVSFK
                     DTLRVYGVPAEWLDCLVITYPDREGNERVQIQLVMRKWSGHLMRYAMALEHQLVQCLD
                     RYEPHVDHSEYEWVWRYGDDCDCPFPDLPAPAEWAQKKVANNVRGTVAPAQPKPMPPV
                     AAKAPPPADDKDFDLRDIFSDLKPEDLRKR"
     gene            3503..5614
                     /locus_tag="PKX82_02520"
     CDS             3503..5614
                     /locus_tag="PKX82_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOF51151.1"
                     /translation="MKHVVSISLGTSSQDFEFTTEFLGEKLHVRRIGTNGNTAEAVKL
                     IKQWDKKAAAIGIGVLKDHYKVGSRRFIEKDSARLKAVATRVPVTTGGRLGDIFQEWA
                     VRHTQTQLGSYFNNARTLFFSGLTDYKLALALSEYTSNLQFADPVLQMGVPKLLNSLD
                     ALHLYSNGAHYVKDWTLPSAIATGPVKEWTRFVLRKALQKATVVVAPVHELDDLGLEE
                     LAGKTLITATVNDERMAQLRDKGVSMVIDGSPTMNGHVLGANLLDAMILAATGKAPQD
                     IQEDDYLEIITGLDSEPRIVYPNGFKRVNRFAFVIHPLSQEYFKKVKPIELLSQVSPP
                     VLMNSIEKIMAYAPPFVYTRVTGIQSPTGVEAEGWLISVGGTPREIMRHDPEFTYRRL
                     LDAANMARRLGAQIMGLGAFTKVVGDAGLTVAKRAPLPITTGNSYSASGALWAAHDAI
                     LRMRLLPIPKGKERVKFKAMIVGATGAIGSVCARLMARAAEEVYMVSPESAKLLALKE
                     SILKDSPGAKIFLSSSADKCIADMDMVVTATSGAGKKVLDIMKVKPGCVITDVARPLD
                     LPASEVAKRPDVLVIESGEIQLPGDVQMKNIGLPKGLAYACLAETIVLALEGRFENFT
                     VGRAIEWEKVREIYQLGLKHGMKLAAISGVNGPFSDADIERVRELALIARAAQKPVKA
                     PAKTAGRVRKPVAKKTTTLSK"
     gene            complement(5676..6464)
                     /locus_tag="PKX82_02525"
     CDS             complement(5676..6464)
                     /locus_tag="PKX82_02525"
                     /inference="COORDINATES: protein motif:HMM:NF018066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HOF51152.1"
                     /translation="MPSPQNDPFFQVPLVSRTTSEGAADFPILYFDARCVQAFFWCPL
                     AAVQARLAGTGLRPAMVWGDRAIVGLAFFEYLNTSIGLYNEAGVAIPVLPQSSKHRTR
                     WLQVLQDVESPQRELGFHVVHLPVTTPAACAAGREIWGYPKFVTDIPFALSPKAVNVA
                     VRDPGDRGLSITVLKGNLGASLPAPTLSLLLYSALDGQKLRTKVNVRGKTRAYAGSDV
                     RLEVGTSTHPMATTLRLLGLDQARPLVLTTTHGFQSRLNLGQPY"
     gene            complement(6492..7877)
                     /locus_tag="PKX82_02530"
     CDS             complement(6492..7877)
                     /locus_tag="PKX82_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF51153.1"
                     /translation="MNPSITLSNAMSALDGHLPATDVLSPRAALQSLSAITRGLMAYQ
                     PDTLWNTETARLPAPLQRLRHRARAFAQQELAPRALQLDALPHWPVGQPPEALKDMLA
                     CAGRAGWLTDMLPAPFGSASWREYRHPLVLRSCIKVEEFSRVCGGQMLLLCAHTLGLA
                     PILLTGNPNDLRRFVLPAFRALQRGEPHLFAFAITEPGAGSDVEEGHGAAQLRPGVVA
                     RRVDGGWHLNGRKCFISGGDMARSLTVFAALEGEGMASWTCFLVRSDMPGFRATRTEL
                     KMGMRASGAAELEFDNVFVPDSHVLGRLRKGWALNRQTLNLSRLPVAAMGVGFAQAAT
                     DIALDYACRTTLGGRALVHDQAVQLCLAQMLAETSTIRALVWQGARTWAPRQSTASMA
                     KFHCTDVAMRVITLAMDLLGSHALDHSQRLEKVYRDCRLTQIFEGTNQINRLALIEDQ
                     QEELLARIPPA"
     gene            complement(7874..9025)
                     /locus_tag="PKX82_02535"
     CDS             complement(7874..9025)
                     /locus_tag="PKX82_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF51154.1"
                     /translation="MESHRLTSEDLAALLETVQRFAQRSLADATAAPHQPMPPATTAA
                     LVGQLLDMGVLNATEEPACGLWDDPQDPLQVEFALHALQTLAHQSPGFAYQVHVQALA
                     NLLSRSTGAPTGPDLVVLEGWLGLGHRALPGTLLGTVSGAATSPEDAARMADCWCLPT
                     ADKPRTVVALPDWSTVWIPTWQADKGWCWHRLARTDLVVQMQAHAHGLDELVPQRISL
                     RAGVVPPAADICAADESSLHFLALSGLYGMGLLAIATGAARRALAIATDYAAMRRQGG
                     PLIQEHDAVAQLLGEARQSVRLADTALASTTQPTTSLALLADIWQLRAALHPLLCTAA
                     SQSMQVLGGIGYMRDQGVEKLLRDCNQLRLLGGSPQELTLCSAQKELLV"
     gene            9133..>9745
                     /locus_tag="PKX82_02540"
     CDS             9133..>9745
                     /locus_tag="PKX82_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOF51155.1"
                     /translation="MPAPALSRELPTDRRMSVLGIANLLDWQAQLGVPAYTLLADTGI
                     DPALLQDPHATLSVRQDMAFTRSLLRHNRMPALGLMVGQQFRLSAFGHLGMMLPLAAT
                     RREAIELFIRYINLSYTHFQIALHLDGEVGKLSFGGADYLGELRPYYLARDMAFAANM
                     ARTLFGGENPLRITQATLAAVPSGERQAYADFFGVPVRYGRGDF"
BASE COUNT         1726 a         3009 c         3240 g         1770 t
ORIGIN      
        1 cagggctggg gtgccgagct ggggctgcag gcggcgatcg ctgcctgcca cgcgcgcgca
       61 ccgaccgttg ttggcgaaag gcaatttgct gccgggccgc cccaaggcaa attagccccc
      121 tcggggggca gcgacccgcg aagcggcgga gcgtgggggc cgtccagtca gttccaggag
      181 ccaatgtccg cattcttgcc ctcgccgccg gcctggccgt cagcgccaaa ggacagcacg
      241 tcaatttcgc cccggatgcc ggggttgagg tactggtagg cgttgcccca ggggtcgttg
      301 ggcagcttgt cgaggtaggg tttccagttg ccgggcactg gtgcggtcgt gggcttggcc
      361 accagggctt gcaggccttg tgccgccgcc gggtagcgct ggttgtccag ccggtagagc
      421 ttgagcgcct gcatcaggtt gtttacatcc gttctggccg ccgtggcgcg ggcgtcatcg
      481 gcacggtcca gcacattagg cacgattaac gcggccagca cgccaatgat caccagcacc
      541 accatcagtt cgatgagggt gaagcctgtg gcgagtccgc gacggacgtt gcgaagatgc
      601 ggcagggtag ggaaagtgat gcgttggctc atggtcaggt caatcataat ccgctaatgc
      661 atacacacgg taacaggatg ttcggggtaa aagcggctac ttttgcgctt tccgcgctgg
      721 cggccgcaag cctggcctac tggggtctga aggtgtttgg tactcctgca ccgggcgctg
      781 tggcaccggt cgccagtgtg gtcggtatgg ccgccattga tccgcaggcc gtggtccgcg
      841 ccctgggggg cggtgaagtg gctgtgccgg tggccgcagt agcgccatcg gcttccagcc
      901 gtttttcgct gaaaggggtg gtggccgata cctccaacgg tggtgccgcg ctgatctcgg
      961 tggacggcaa gcctgccagg ccctttcagg ttggcgactt ggtggatggg cggctggtgg
     1021 tgcagtcggt caccggacgc aaggcatcgc tggcagccgg cctgagtgcg cctgtggaag
     1081 tggtgctgga gctgccgccg ttgccccaat gatgcgatct gcgccaccgg ccaggaatcc
     1141 agctcttgat gccctgcgcg gttttgccgt gctgtggatg acggcgtacc atttttcgtt
     1201 tgacctcaac cactttggcc tgatccgcca ggacttttat accgatccgg tgtggaccgt
     1261 gcagcgtacc tgcatcctga gcctgttcct gctgtgtgct ggcggtggcc aggccatggc
     1321 ggtggccagc gggcagagct ggcggggctt ttggcgccgc tggagccaga tcgcgggttg
     1381 tgcggtgctg gtgagtgtgg gctcgtattt catgttcccc aacagctgga tctactttgg
     1441 tgtattgcac ggcatggctg tgatgctggt ggtgtgccgg ttgacggcca gctggggcca
     1501 cgggctgtgg ctggccggtg cggtagctgt cgctctgggc cagtggggcc cacaactgca
     1561 cgccctgtgg ccgtccgcag aggtgctgaa cgggcgtggg ctgaactggc tggggctgat
     1621 aagccgcaag cccatcaccg aagactatgt gccgctgttg ccctggatgg gggtgatgtg
     1681 gtggggcatg gcggcagcgc aactggcgct ggcacgtggc tggctgctcc ccgcgcagcg
     1741 gcggagcgtg ggggcacgca tgggcttggc gttcgtggga cgctggagcc tgtcctggta
     1801 catggtgcac cagccgctgc tgatgggcct tttgacgctg tggcgtgcct tgtaagtaaa
     1861 gagatgagcg cgttttttca caacgtggta cattcgggga tcgcttttgg cgactgaccg
     1921 cgtccatgca ttgctgcaac gacgcaaacg ctcccggcag aggagcgatc agtccggccc
     1981 ttcaattgcg ttcacgctat ccatctcacg gcatagctgc tttttccctc tgttgacgat
     2041 gcccctgccg ggcgcgtgcc ttgtaccggt cctgtgtgga ctggcaaacg ggttgacagg
     2101 acgggaccct cggcatgagg gcatgccagt gtttgctttc ggtttttaat gatttttact
     2161 acaactacaa acgagattcc tgtcactatg ggcaagtacc gcatcgcggc ctgtcctgaa
     2221 aagttgccgt gcggccaata tgccgcacag gtatcgattg ccagcggacg cggtagcgcg
     2281 tccaccgacc gcgtgatgcg ttttcacgac gaatttgcca cgcacgatgc cgcatcgcgg
     2341 ttttccatcg cgcaaggcat agactgggta cgtgccacca cgcgccccca ctgattcact
     2401 tagaggacca caccatggcc aaagaagaac tgatcgaaat gatgggcgtt gttaacgaag
     2461 tgctgcccga tacccgtttc cgcgtcacgc tggaaaacgg gcaccagctc atcgcctact
     2521 ccgccggcaa aatgcgcaag aaccacatcc gcatcctggc gggtgaccgc gtgtcgctgg
     2581 agttgtcgcc ttacgacctg agcaagggcc gtatcaactt ccgccacatc gaaggccgcg
     2641 gtcccatgac accgcgccgc acgaactaag ccggtacctg cgtcctcgcc gatgtccgga
     2701 ccacccgaat gggggatgga caatcgcacc attgcgtatt aacgtcaaaa ccgggacgcg
     2761 cagtacaaat acagcagttt gaggtcaggg tatgttcaag ttttcacggg catcgaaagc
     2821 caaaccggat tcgcagtttg ccgagaccac catggtcacg gcaggtggat ccacggtggc
     2881 tgcgggcggt ttccagcggg acttgatcta tgtgtctttc aaggacaccc tgcgcgtcta
     2941 tggcgtaccg gccgaatggc tggattgcct ggtcatcact tacccggacc gtgaaggcaa
     3001 cgaacgcgtc cagatccagc tggtgatgcg caagtggagc gggcacctga tgcgctacgc
     3061 catggccctg gagcaccagc tggtgcaatg cctggaccgc tacgaaccgc atgtggacca
     3121 ctcggaatac gagtgggtct ggcgatacgg tgacgattgc gattgcccgt tccccgacct
     3181 gcctgccccc gcagaatggg cccagaaaaa agtagcaaac aacgtgcgcg ggacggtagc
     3241 gcctgcacag cccaaaccca tgcccccggt tgccgccaaa gccccgcctc ctgcggacga
     3301 caaggacttt gacctgcgcg acatcttctc cgacctgaaa cctgaagacc tgcgcaagcg
     3361 ctgaaattgc taccaatttc gtagcttatc acgcttgccc gacgcgggct acagcccaaa
     3421 atgatgacta tttgagccaa tcggggttga cacgggcgcc gaggtgaaag aatgacagga
     3481 gtcaattttt accggcacca ccatgaaaca cgttgtcagc atcagcctgg gcacgagcag
     3541 ccaggatttt gaattcacca cggaatttct cggcgagaag ctgcatgtgc ggcgcatcgg
     3601 caccaatggc aataccgcgg aagccgtcaa actgatcaag cagtgggaca agaaggccgc
     3661 agccatcggc attggcgttc ttaaagacca ctacaaggtg ggttcgcgcc gcttcatcga
     3721 gaaagacagc gcgcgcctca aggcggtggc cacccgtgta cccgtgacca cgggcggccg
     3781 gctcggcgat atcttccagg agtgggccgt gcgccataca cagacccagc tgggcagcta
     3841 cttcaacaat gcgcgcaccc tgttcttctc gggcctgact gactacaagc tggcgctggc
     3901 gttgtcggag tacaccagca acctgcagtt cgccgacccg gtgctgcaga tgggtgtgcc
     3961 caagctgctg aacagtctgg acgcgctgca cctgtactcc aacggcgcgc actatgtcaa
     4021 agactggacc ctgccctcgg ccattgccac cgggcccgtc aaggagtgga cgcgcttcgt
     4081 actgcgcaag gccctgcaga aggccaccgt ggtggtggcg ccggtgcatg aactcgacga
     4141 cttgggtctc gaagagctgg ctggcaagac cctgatcacc gccaccgtca acgacgaacg
     4201 catggcgcag ctgcgcgaca agggcgtgag catggtgatc gacggttcac ccaccatgaa
     4261 cggccatgtg ctgggcgcca acctgctcga cgccatgatt ttggcggcca cgggcaaggc
     4321 accgcaggat atccaggagg acgactacct cgaaatcatc accggcctgg actccgagcc
     4381 gcgcatcgtc taccccaacg gcttcaagcg cgtgaaccgc tttgcgttcg tgatccaccc
     4441 gctgtcgcag gagtatttca agaaggtcaa accgattgag ctgctgtcgc aggtgtcgcc
     4501 accggtgctg atgaactcga tcgaaaagat catggcctac gcgccaccgt ttgtgtacac
     4561 ccgcgtcacc ggtatccagt cccccacggg cgtggaggcc gagggctggc tgatctcggt
     4621 cggaggcaca ccccgggaaa tcatgcgcca cgacccggag ttcacctacc gccgcctgct
     4681 cgacgccgct aacatggccc ggcggctggg cgcgcagatc atggggctgg gtgcctttac
     4741 caaggtggtg ggcgacgccg gcctcacggt ggccaagcgc gcgccgctgc ccattaccac
     4801 cggtaacagc tacagcgcct cgggcgcttt gtgggctgcg cacgatgcga tcctgcgcat
     4861 gcgcctgctg ccgatcccga aaggcaagga gcgcgtcaag ttcaaggcca tgatcgtggg
     4921 cgcgaccggg gccattggct cggtgtgcgc acgcctgatg gcgcgcgcgg ccgaagaggt
     4981 gtacatggtg tcgcccgagt cggccaagct gctggcgctg aaggagtcca tcctcaagga
     5041 ctcgcccggt gcgaagattt tcctgtcctc atcggccgac aaatgcattg ccgatatgga
     5101 catggtcgtc accgccacct cgggcgcggg caagaaggtg cttgacatca tgaaggtcaa
     5161 gccgggttgt gtgattaccg atgtggcccg cccattggat ttgcccgctt ccgaggtggc
     5221 caagcgcccc gacgtgctgg tgattgaatc gggcgaaatc cagctgcccg gcgatgtgca
     5281 gatgaagaac atcggcctgc ccaaaggcct ggcctacgcc tgcctggctg agaccattgt
     5341 gctggcgctg gaaggccgct tcgagaactt caccgtgggc cgcgccatcg agtgggagaa
     5401 ggtgcgcgag atctaccagc tcggcctcaa acacggcatg aagctcgccg ccatctcggg
     5461 cgtcaacggc ccgttcagcg atgccgatat cgagcgcgtg cgcgaactgg cgctgattgc
     5521 acgcgctgcc caaaagcccg tcaaagcccc tgcaaagacc gcaggcaggg tacgcaaacc
     5581 ggtggcaaag aaaaccacaa cgctatcaaa ataatagctg attgtgcata ttccacgagg
     5641 gctagagcca gtttttgcca aatatttgtc ggctctcagt aaggttgacc caggttcagc
     5701 cgtgactgga agccatgcgt ggtggtcagt accagcgggc gtgcctggtc caggcccagt
     5761 agccgcagcg tggtcgccat ggggtgggtg gaggtgccca cctccaggcg cacgtccgag
     5821 ccggcatagg cgcgcgtttt gccacgtacg ttgaccttgg tgcgcagctt ctgcccatcc
     5881 agcgccgagt agagcagcag gctcagcgtg ggcgctggca ggctggcgcc caggttgcct
     5941 ttgagcacgg tgattgacag gccacgatcg ccagggtcgc gcacggccac attcaccgcc
     6001 ttgggtgaca gcgcgaatgg aatgtcggtc acaaacttgg ggtagcccca gatctcgcgc
     6061 ccggcggcgc aggccgctgg tgtggtgaca ggaaggtgca ccacgtgaaa gcccagctcg
     6121 cgctgcgggc tctccacgtc ctgcagcacc tgcaaccagc gggtgcggtg cttgctgctc
     6181 tgcggcaata ccgggatcgc aacccccgcc tcgttgtaca gcccgatcga ggtgttcagg
     6241 tattcaaaga acgccagccc cacgatggcg cggtcacccc acaccatggc cggacgcagg
     6301 ccggtgccgg ccagacgcgc ctgcactgcg gccagcgggc accagaagaa ggcctgcaca
     6361 cagcgcgcat caaagtacag gatggggaag tccgccgcgc cctcgctggt ggtgcggcta
     6421 accaggggaa cctggaaaaa gggatcgttc tggggggatg gcatggtgtt gtctcctgtg
     6481 gtttttgtgg tctatgccgg gggaatgcgg gccagcaact cttcctgctg gtcttcgatc
     6541 agggccagcc ggttgatctg gttggtgcct tcaaagatct gggtgaggcg gcagtcgcgg
     6601 tacaccttct ccagccgttg gctgtggtcg agcgcgtggc tgcccagcag gtccatggcc
     6661 agcgtgatca cgcgcatggc gacatcggtg caatggaact tggccatgga tgcggtggac
     6721 tggcgcggcg cccaggtgcg cgcgccctgc cacaccagcg cgcggatggt gctggtctcg
     6781 gccagcatct gcgccaggca cagttgcacc gcctggtcgt gcaccagcgc ccgtccgccc
     6841 agcgtggtgc ggcaggcgta gtccagcgca atgtcggtgg cggcttgcgc gaagcccacg
     6901 cccatggcgg ccaccggcag gcgcgagagg ttcagtgtct ggcggttcag tgcccagccc
     6961 ttgcgcagcc tgcccaacac gtgagagtcg ggcacgaaca cgttgtcgaa ttccagctcg
     7021 gccgcgccgg aggcgcgcat gcccatcttc agctcggtgc gcgtggcgcg aaaaccgggc
     7081 atgtcgctgc gcaccagaaa gcaggtccac gaggccatgc cttcgccttc aagcgcggca
     7141 aacacggtga ggctgcgcgc catgtcgccg ccgctgatga aacacttgcg cccgttcagg
     7201 tgccagccgc catcgacccg tcgcgcgacc acgccggggc gcagctgggc tgcgccatgg
     7261 ccctcttcca catccgaacc ggcaccgggc tcggtgatgg caaaggcaaa caggtgtggc
     7321 tcgccgcgct gcagcgcacg gaacgccggt agcacaaaac gccgcaggtc attggggttg
     7381 ccggtgagca ggatgggcgc cagcccaagc gtgtgcgcac acagcagcag catctggccg
     7441 ccgcagacgc ggctgaattc ttccaccttg atgcagctgc gcagtaccag cggatggcgg
     7501 tattcgcgcc aggatgccga gccgaagggc gcgggcagca tgtcggtgag ccagcccgcg
     7561 cggcccgcgc aagccagcat gtctttcaag gcctccggag gctggcccac cggccagtgg
     7621 ggcagggcgt cgagttgcag cgcgcgcggg gcgagctcct gctgtgcaaa agcccgggca
     7681 cggtggcgca ggcgttgcag cggtgcaggc aggcgcgcgg tctcggtgtt ccacagggtg
     7741 tcgggctggt aggccatcag gccgcgcgtg atcgccgaca gggactggag tgcggcgcgt
     7801 ggcgacagca catcggtcgc aggcaggtgc ccgtccagtg cagacatggc gttagacagg
     7861 gtgatcgatg ggttcatacc agcaactcct tctgcgcact gcacagcgtg agctcctgtg
     7921 gcgacccgcc cagcaggcgc agctggttgc agtcgcgcag cagtttctcc acgccctggt
     7981 cgcgcatgta gccgatgcca ccgagcactt gcatggactg gctggccgca gtgcacagca
     8041 gggggtgcag tgcggcgcgc agttgccaga tgtcggccag caacgccagg ctggtggtgg
     8101 gctgcgttgt gcttgcgagc gcagtgtcgg caaggcgtac cgactgacgg gcttcgccca
     8161 gcagctgggc cacggcatcg tgctcctgga tcagcggtcc gccctggcgg cgcatggcgg
     8221 cgtagtcggt ggcaatcgcc agtgcgcggc gggccgcacc cgtggcaatg gccagcaggc
     8281 ccatgccata caagccagag agcgccagaa agtgcaggga ggactcgtcc gcagcgcaga
     8341 tgtctgcagc cgggggaacg acgcctgcgc gcaggctgat gcgctggggt accagctcgt
     8401 ccagtccgtg cgcatgggct tgcatctgca ccaccaggtc ggtgcgtgcc agtcggtgcc
     8461 agcaccatcc tttatcggcc tgccaggtgg gaatccagac cgtggaccag tccggcagcg
     8521 caactacggt gcgcggcttg tcggcggtgg gcaggcacca gcagtcggcc atgcgtgcgg
     8581 catcctcggg gctggtggca gcaccagaca cagtgcccag caaagtaccg ggcaaggccc
     8641 ggtgccccag gccgagccag ccttccagca ccaccaggtc gggcccggtg ggtgcgcccg
     8701 tgctgcgcga cagcaggttg gccagcgcct gcacatgcac ctggtaggca aagcctggtg
     8761 actggtgtgc gagcgtctgc agggcgtgca gggcaaattc cacctgcagt gggtcctgcg
     8821 ggtcgtccca gaggccgcag gcgggttctt cggtggcgtt aagcaccccc atgtcgagca
     8881 gctggcccac cagtgccgct gtggtggcgg gtggcatggg ttggtggggg gccgcggtcg
     8941 cgtcggccag gctgcgctgg gcaaaacgct ggacggtctc cagcagtgcg gccaggtcct
     9001 cggaggtcag tcggtgcgat tccatcggcg catcgtaggg acgggggccc cggagaggaa
     9061 tacccgatca ggaaataaat ttgatcattc cggacacatc ggctagattg cgcccttcac
     9121 ctccacttgt ccatgcccgc acccgccttg tccagagaac tgcctaccga ccgccgcatg
     9181 tcggtgctgg gcatcgccaa tctgctggac tggcaggcgc aactgggtgt tccggcgtat
     9241 accctgctgg ccgataccgg catcgatccc gcactgttgc aagacccgca cgccaccctc
     9301 agcgtgcgcc aggacatggc cttcacccgc agcctgctgc gccataaccg catgcctgcg
     9361 ttgggcctga tggtggggca gcagttccgc ctgtcggctt ttggccacct gggcatgatg
     9421 ctgccgctgg cggccacgcg gcgcgaggcc atcgagctgt ttatccgtta catcaacctg
     9481 agttacaccc acttccagat tgcgttgcac ctcgatggtg aggtgggcaa actgagcttc
     9541 ggtggtgccg attacctggg cgagctcagg ccttactacc tcgcgcgcga catggcgttt
     9601 gcggccaaca tggcacgcac cctgtttggt ggcgagaacc ccctgcgcat cacgcaggcc
     9661 acgctggcgg cggtgcccag tggtgagcgc caggcgtacg ccgatttctt tggtgtgccg
     9721 gtgcgctatg gccggggaga ctttc
//
LOCUS       DAOSJM010000041         9493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6934_length_9493_cov_4.446069, whole genome shotgun sequence.
ACCESSION   DAOSJM010000041 DAOSJM010000000
VERSION     DAOSJM010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6934_length_9493_cov_4.446069"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PKX82_02545"
     CDS             <1..318
                     /locus_tag="PKX82_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF51156.1"
                     /translation="AFAGDDDGTPVGLVNCIEGFSTFACKPLVNVHDVAVLAGYRGQR
                     IGERMLELVETLARERGACKLTLEVLSGNAGAMRLYQRTGFAHYQLDPAMGQAGFMQK
                     WLD"
     gene            complement(324..1148)
                     /locus_tag="PKX82_02550"
     CDS             complement(324..1148)
                     /locus_tag="PKX82_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOF51157.1"
                     /translation="MSTGFSAPDREAVQSLLGKAFGNFRTHLDLLQESNDPEVVHQAR
                     VSWRRFKSAMHLFKPLLQKDGIPANGALQPMLKALSRLRDLDVARFQTLPKLSSAYLE
                     GAPAHTQEWERVTRVFASADRQQRLALRAVLREPVVQGTLEDTSQWIAKLASHSELFV
                     ARDHADGALRSWARHRVHQMHRTMRKAKANADTAQSQHRVRILAKHVRYSIEMLRDVF
                     PHKTAEKWHLEAIDLQTRIGQTRDMVRANALARELELPSDIAERLQSIASSPTGHE"
     gene            complement(1123..1404)
                     /locus_tag="PKX82_02555"
     CDS             complement(1123..1404)
                     /locus_tag="PKX82_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51158.1"
                     /translation="MHRRINHLSQVFQEMKDRYGSDDPVVLQLKEAIDKYNAVDSLPA
                     DAALPFGERRTIRTKPSYWNVKLRHMHQPLSRHDILAECKRIEHRVFSA"
     gene            1610..3565
                     /locus_tag="PKX82_02560"
     CDS             1610..3565
                     /locus_tag="PKX82_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOF51159.1"
                     /translation="MLKEKAVASLGQQSLLLPAWVKAALLANDRLKLYLTMLQAAAQH
                     AGTPETPLTDWGRDLAQAGLHDAGWLQELVKTAYFDDRLLLIPQLDQLTRALAGDLLT
                     MARPLCEGSSHQDAELTGRRDGWLQRLRTMAEEEGLSPDALADLTRGDRKGGDSFHIL
                     VMDIHKRLNAMSGEIATESIDGAHVWQVEPSDKALIQSFMRGLNRTAPLKFSHPGLDT
                     AVTRDGPRLLIQNDIGTNDVHVLVIEVEEGTIRLHYSDLHPGRFDFFRQMLEGAGFEW
                     TVSDPMTSDGLNAGKPYTLGTAVLTTTDPIALANGLEAVGARIVFVIDWNRARKRLQS
                     FVRKPQAIALLRRAADQEWGHMAWLLAGGDRLVFNAMQAVDSEAFRIGDRLDEVLGEE
                     SATAFLLELMRIASVMLRQQLPMGMVADEARMLLARVLRKRTFEFDLLAEHAAYCHAL
                     ALALCSALEDGSTVSAEQNSNLVLRAKAWERKADHLLMEARQRAERQLRWVPVVELLE
                     KSDDVADALEEATFIFSMTRLDSLAGLPPIVQSALRRLADTTLAAIQDQIKAIEIARR
                     VSESGEPADSELFLQTLWRMLRAERLCDELSRQARMKIVVALASVPVNLLLASDLANT
                     IEKASDGLLAAGHALRQMVINKTGISA"
     gene            3562..5193
                     /locus_tag="PKX82_02565"
     CDS             3562..5193
                     /locus_tag="PKX82_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOF51160.1"
                     /translation="MKSSSHWPKHIYLIASGEATHQHKATRESMGSKAYNLLRMAEIG
                     LTVPPALVIGTHFTRTPDECMLPLFAVGLAALQDVSGLVFGDARNPLIVSVRSGAPVS
                     MPGMMETLLNIGLCDSTAPGLLRQTGNPRLVWDAYRRLIASYAEVVAGLPGSLFEAEI
                     NRTTGGRDERELDFSELRELTARFKTIYQQNAGSAFPQDVNEQLSGAVRAVFASWNSD
                     KAQAYRDINGIDAAMGTAVTIQAMAFGNSGGHSGAGVGFTRDPTTGDRKLWVDFLANA
                     QGEDVVSGRRNAHGHQAMADLVPGAWRSLQEAVQALERAFGDMQDFEFTVQEGVLHML
                     QTRTGKRTPLAAARIAMDLLEDGLIDAATAHERTAGLQESDMGRVQIAPVDDKQTPVS
                     PCARAATACPGVVSGEIALDGERAAARAAQGVQVVLVRQDAETSDIVALESAVGLLTQ
                     RGARTSHAAVVARQLGRVCLVGCDNLHIESEARTIKLGDLVLHEGDTITLDGNEGAVY
                     DGVVAAVMVPDAALVSRLRALRASHAAGHQRRHGA"
     gene            5199..5967
                     /gene="fabI"
                     /locus_tag="PKX82_02570"
                     /pseudo
     CDS             5199..5967
                     /gene="fabI"
                     /locus_tag="PKX82_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700901.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
     gene            6011..7420
                     /locus_tag="PKX82_02575"
     CDS             6011..7420
                     /locus_tag="PKX82_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOF51161.1"
                     /translation="MYTFAPQEYTDNVTYDEIQIGQTARIVRTLTLEDIHAFAAVSGD
                     TNPAHLDPTYANATLFHGVIAHGMWGGALISALLGTVFPGPGTIYLEQALHFSKPVRI
                     GDTLTVTVMVTAKDDAKKRVELDCQVLNQRGEGVLHGMARVLAPTQRVRSPRVNVPQI
                     QLFDPEARLRALLSLGDGLPAVRCGIVHPCDIDSLGGAIDAARHGLIDPVLIGPEAKL
                     RELAEEAGIDLAGIAIEAVPHSHAAALRAAELATDRKVEALMKGSLHTDEMIHAVLAN
                     PALRTARRMSHVFRFDVPTYAKPLLLTDAALNIRPSLADKVDIVQNAIAFARIMGTAL
                     PKVAVLSAVETINPEIPSTLDAAALCKMAHRGQISGGLLDGPLAFDNAISAHAARIKH
                     IESDVAGDADILMVPDLESGNMLGKQLQYLAGATASGIVLGARVPIALTSRADGVSAR
                     MASALLAKMIAHHNRMIQP"
     gene            7417..8598
                     /locus_tag="PKX82_02580"
     CDS             7417..8598
                     /locus_tag="PKX82_02580"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOF51162.1"
                     /translation="MTVLAVNAGSSTLRFALHPVEGGIAQPALLTGVIEGLSAQGPLD
                     MIWNGGGTTHTERSGDADGDGFESALAMLQKMLQRESAGRELQAVAHRVVHGGARYRE
                     ATVVTDSVVQDLERLCRLAPLHQPHNVQGIRAFARRFPTVPQVACFDTAYHADLPDVE
                     YRFALPQALHDEGVRRYGFHGLSYQYVMQRLEQTTCMAHGRVVMAHLGSGASVCGATQ
                     GQSRATSMGFSALDGLMMGTRAGALDAGVVLHLLAQGWDHARLQHLLYRQSGLLGVSE
                     VSSDMRQLRRSDSAQADLAIRMFTYRVVREVGAMVACIGGLDVLAFTGGIGEHDATLR
                     SEVCERLAFLGVRMDPRANALADGSGPVALHLPDSAVEVWMVPTDEGFVAAQTAVRLV
                     L"
     gene            8923..>9493
                     /locus_tag="PKX82_02585"
     CDS             8923..>9493
                     /locus_tag="PKX82_02585"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOF51163.1"
                     /translation="MTTLPAVTYGADASGLICGFQFGEAGPGTAIGAEDALRWLEQNA
                     DAPTAGFVWLHFNLAHTATEKWLREHVHLSNAFHESLKEGSRSTRVEVDDDMLVAVVN
                     DVAYDFAFEPGDISTMWLTVGPRLVISARMQPLRSIDRLRDAVKKGITLRSSVELLVH
                     LMHDQATCWSILCAPPQRGLTAWRTICCPV"
BASE COUNT         1769 a         2704 c         3063 g         1957 t
ORIGIN      
        1 gcgtttgcgg gtgacgacga cggtacgccg gttggtctgg tcaattgcat cgagggcttt
       61 tccacctttg cctgcaagcc cctggtcaat gtgcacgatg ttgcggtcct tgcgggctac
      121 cgtggccagc gcatcgggga gcgcatgctg gaactggtgg aaacgctggc ccgcgagcgc
      181 ggtgcctgca agctgacact ggaagtgctc tctggcaacg caggggccat gcgcctgtac
      241 cagcgcacag gctttgccca ttaccagctg gacccggcca tggggcaggc gggcttcatg
      301 cagaagtggc tggactgaac gcgctattcg tgcccggttg ggctgctggc aatgctttgc
      361 aggcgctctg caatgtcgga cggtagctcc agctccctgg ccagggcatt tgcccgcacc
      421 atgtcgcgcg tctgtccaat gcgcgtttgc agatcgatcg cttcaaggtg ccatttttcc
      481 gccgtcttgt gcggaaaaac gtcgcgcaac atctctatgc tgtagcgtac gtgtttggca
      541 agaatgcgga cccgatgctg actttgcgct gtatctgcgt tggccttggc tttacgcatt
      601 gtcctgtgca tttggtggac tctgtgcctg gcccaggaac gcaaggcacc atcggcatgg
      661 tcacgggcaa cgaaaagttc actgtgcgaa gccaatttcg caatccattg cgatgtgtcc
      721 tccagagtgc cttgaacaac tggctcccgc agcaccgccc gaagggcaag tcgttgttgc
      781 cggtctgcgc ttgcgaaaac ccgtgtcacg cgttcccact cctgcgtatg tgccggggct
      841 ccttccaagt aggcgctgga taacttcggt aatgtttgaa agcgggccac atccaggtcc
      901 cgcagccggc tgagcgcttt gagcatcggt tggagtgcgc cattggctgg aatgccatcc
      961 ttttgaagca ggggcttgaa aaggtgcatt gcgctcttga atcggcgcca gctgactctg
     1021 gcctggtgca cgacttcagg atcgttggac tcctggagca aatccaggtg ggtgcggaaa
     1081 tttccgaatg ctttgcccag cagggactgg acagcttccc ggtcaggcgc tgaaaacccg
     1141 gtgctcaatg cgtttgcatt cggcaaggat gtcgtgccgg ctcaagggct ggtgcatgtg
     1201 tctcagtttc acgttccagt agcttggctt ggttcggatg gtccggcgtt caccaaacgg
     1261 aagtgcggca tccgctggaa gcgaatcgac ggcgttgtac ttgtcgatgg cctctttcag
     1321 ttgaagaact accgggtcgt ccgaaccata gcggtccttc atttcctgga acacctggct
     1381 caagtggttg atacgtcgat gcatgcggta tctccttgaa agacggtaga ccagtatcac
     1441 agaaacctgt gacgatttga agacgcttat tcccttggtg ctgagggcaa ggccattttg
     1501 acaaatatca acgcaatgcg gttttcagcg ccattgcgcg ggttgtcaca ggccagccat
     1561 atccgtttct gagaatcgtc tgcatcagac attacgaacc ggacattgca tgctcaaaga
     1621 aaaagccgtg gcctctctgg ggcagcaatc gctgcttctg cccgcctggg tcaaggcagc
     1681 actattggcg aacgaccgtt tgaagttgta cctgaccatg ctgcaggctg cagcgcagca
     1741 tgcggggacg ccagagacac cgctgacgga ttgggggcgg gatctggcac aggcgggtct
     1801 gcacgatgcc ggatggttgc aagaactcgt caagacagcg tactttgacg atcgactgtt
     1861 gctcattccc cagctcgatc aactcacccg ggcactggcc ggtgatttgc tcaccatggc
     1921 gcggcccctg tgtgaaggaa gcagccacca ggatgcagaa ctgacgggca ggcgcgatgg
     1981 ttggctgcaa cggctgcgca ccatggccga agaggaaggg ctttccccgg acgcactggc
     2041 ggatctgacg cgtggcgacc gcaagggcgg cgacagtttc catattctgg tgatggatat
     2101 tcacaagcgg ctcaatgcca tgtccggtga aatcgccacg gaaagtattg acggtgcaca
     2161 tgtctggcag gtggagccct cggacaaagc cctgattcag tcctttatgc ggggcctcaa
     2221 tcggacggca ccgctgaagt tctcccaccc gggcctggat actgcagtta cccgcgatgg
     2281 cccgcgtttg ctgattcaga acgacatcgg caccaacgat gtgcatgtac tggtgattga
     2341 ggtggaagag ggcaccatcc ggctccatta ctccgatctg cacccggggc gcttcgactt
     2401 tttccggcag atgctggaag gtgcaggctt tgagtggacc gtgtcggacc cgatgacctc
     2461 ggacggactc aacgccggca agccctatac gctgggcaca gcggtgttga caactacaga
     2521 cccgatcgcg ctggctaacg ggctggaggc cgtgggcgcg cgcattgtgt ttgtgatcga
     2581 ctggaaccgg gcgcgcaagc ggttgcagag ttttgtgcgc aagccgcagg ccattgcctt
     2641 gctcaggcgt gctgccgatc aggagtgggg gcacatggcg tggctgctcg caggtgggga
     2701 caggctggtg ttcaatgcca tgcaggctgt cgatagcgag gcctttcgca ttggagatcg
     2761 cctggacgaa gtactgggag aagaatccgc taccgccttt ttgctggagt tgatgcgcat
     2821 tgccagtgtc atgctgcgcc agcagcttcc gatggggatg gttgccgatg aagcgcgtat
     2881 gttgctggca cgcgtcctgc gaaagcgcac tttcgagttt gatctgctgg ctgaacatgc
     2941 cgcctactgc catgcgcttg cgctggcatt gtgctcggcg ctggaagatg gctccaccgt
     3001 gagtgcggaa cagaacagca acctggttct tcgtgccaag gcctgggaac gcaaggccga
     3061 ccatttgctg atggaggcgc gccagcgggc cgagcggcaa ttgcgctggg tgccggtggt
     3121 ggagttgctg gaaaagtcgg atgacgtcgc ggacgcactg gaagaagcga ccttcatttt
     3181 ttccatgacg cggctcgact ccctggcagg gctgccgcca atcgtgcagt ccgccttgcg
     3241 gcgcctggcc gacaccacgc tggcagcgat tcaggaccag atcaaggcga tcgagattgc
     3301 ccggcgtgtg agcgaaagcg gagagcctgc cgacagcgag ctcttcctgc aaacgctgtg
     3361 gcgcatgttg cgcgcagaac gtctgtgtga cgagctttcg cgccaggccc ggatgaagat
     3421 cgtggtggcg cttgcgtctg tgcctgtcaa cctgctgctg gccagcgatc tggccaacac
     3481 cattgaaaag gcttccgatg gattgctcgc cgctggacat gctttgcggc agatggttat
     3541 caacaagact ggaatatccg catgaagtcc tcctcccatt ggcccaagca catctatctc
     3601 attgccagcg gtgaggcaac gcaccagcac aaggcgaccc gcgaaagcat gggctccaaa
     3661 gcctacaacc tgcttcgcat ggccgagatt ggcctgacag ttcctccagc actggtcatc
     3721 ggaacgcatt tcacccgcac tcccgatgag tgcatgctgc cgcttttcgc tgtaggcctg
     3781 gcagcattgc aggatgtttc cggactggtg tttggcgatg ccagaaaccc gttgatcgtt
     3841 tctgtgcgtt ccggggcacc ggtatcgatg ccaggaatga tggagacact gctcaacatt
     3901 ggcctgtgcg acagcaccgc ccccgggctg ctgcgccaga cgggtaatcc gcgcctggtg
     3961 tgggatgcct accggcgtct gatcgccagc tatgccgaag tggtggcggg actgccggga
     4021 tcgttgtttg aagcggagat caatcgcact acgggggggc gcgatgaaag agagctggac
     4081 ttcagtgagc ttcgcgagct cactgcgaga ttcaaaacga tctatcagca aaatgcagga
     4141 tctgccttcc cccaggatgt gaatgagcag ctgtctgggg ctgtccgtgc ggtgtttgcc
     4201 tcatggaatt ccgacaaggc gcaggcgtat cgcgatatca acggcattga tgccgccatg
     4261 ggtaccgccg tcaccatcca ggccatggcg tttggcaaca gcggcgggca ttccggcgcg
     4321 ggtgttggct ttacgcggga cccgaccaca ggcgacagaa agctgtgggt ggatttcctg
     4381 gccaatgcgc agggcgagga cgtggtgtcg ggccggagaa acgcacatgg acaccaggcc
     4441 atggcggacc tggttcccgg ggcctggcgc agcctgcagg aagcggtgca ggcgctggag
     4501 cgtgcattcg gtgatatgca ggactttgag ttcacggttc aggaaggcgt tctgcacatg
     4561 ttgcagaccc gaaccggcaa gcgtacgccg ctcgccgctg cgcgcattgc gatggacctc
     4621 ctggaagatg gcctgattga tgcagcgacc gcgcacgagc gtacggccgg gttgcaggag
     4681 tccgatatgg ggcgggtaca gattgccccg gtcgatgaca agcagacgcc agtcagtccc
     4741 tgtgcacgtg ctgctacggc ctgcccgggt gtggtctccg gggagattgc gctggatggc
     4801 gaacgtgctg cggcgcgggc cgcacagggc gtgcaggttg ttctggtgcg tcaggatgcc
     4861 gagacgagcg acatcgttgc actggagtcg gccgtggggt tgctgaccca gcgcggtgcg
     4921 cgcacgtccc atgctgcggt ggtggcccgc cagctggggc gtgtgtgcct ggtgggctgt
     4981 gacaacctgc acatcgaatc ggaagcgcgc accatcaagc tgggagacct ggtactgcat
     5041 gagggtgaca ccatcacgct ggatggcaat gagggggctg tctacgacgg agtggtcgcg
     5101 gcggtcatgg tgccagacgc ggcgctggtt tcgcgcttgc gggctttgcg ggcatcgcat
     5161 gcagcaggcc accagagaag gcacggagca tgagccagat gccaatcatg tcgtcgcttg
     5221 ccggcaaaaa agggctgatt gtcggattgg caaatgccca cagcatcgcc tatgggtgtg
     5281 ccagagaagc tgcgctgcaa ggggccgaat tggtgacgtc ctgcctgaac cagaaggcct
     5341 atccgtacgt ggagcccctg gcgcgcgaac tcggagtccc gctgttgacc tgtgacgtgg
     5401 aaacacccgg agaaatgcag tcgctgattg agcgtacggt agaacacctc gggcacctgg
     5461 acttcgtcat ccactccatc gcctgggcac cgctggaaga tctgcacggc gacgtggtca
     5521 acagttcgag tattggcttt gcccgcgcca tggaagtgtc gtgtcattcc tttgcgcaac
     5581 tggcacagct ctgtaccccc catatgacgc agggtggcag ctttgtcacc atgagctatc
     5641 tgggcgctgc agaggcggta cctcactatg gtttgatggg gccggtcaag gctgcgctgg
     5701 agtccctggt gcgctacatg gcactcgaat tggggcccaa aggaatccgc gtacacgccg
     5761 tgtccccggg gcccatactg acgcgcgcag cgtcaggcat tagcctttga cgatctcatg
     5821 acccatgccc agaagaaggc tccgctggga cggctggtca ccctggagga aattgcgcgc
     5881 ctgagtgcgt tcctgtgcag cgaggcttcg tcaggcatga cggggcaaac catctatgtc
     5941 gatgcgggct gccatgccgt cgcctgaacg ttccccccta atcccgcaag ctcaaccgct
     6001 ggaagttccc atgtatacct ttgcccctca ggaatatacc gacaacgtca cttacgacga
     6061 gatacagatc ggccagaccg cccgcattgt gcgaacgctt acgctggaag atatccatgc
     6121 ctttgccgct gtttcggggg acaccaatcc tgcgcatctg gaccccacct acgcgaacgc
     6181 gacgctgttc catggggtga ttgcccacgg aatgtgggga ggtgcgttga tctcggcctt
     6241 gctcggaacc gtcttcccag gcccgggcac catttatctg gagcaggcat tgcatttcag
     6301 caagccggtt cgcattggcg acacgttgac ggtcaccgtc atggtgacgg ccaaagacga
     6361 tgccaagaag cgcgtcgagc tggactgcca ggtgctcaat cagcgtggcg agggggtgct
     6421 gcacggcatg gcccgcgtgc tggcgccgac ccagcgggtg cgtagcccgc gtgtcaatgt
     6481 gccccagatc cagctcttcg atcccgaggc gcgcctccgt gcgttgctca gcttgggcga
     6541 tggcctgcct gccgtacgct gcggcatcgt gcatccctgt gacatcgatt ccctgggggg
     6601 cgccatcgac gcggcccggc atggcctgat cgaccccgtg ctgatcgggc ccgaggccaa
     6661 gctgcgcgag ctggctgaag aggcaggtat cgacctggcg ggcatcgcca ttgaagcggt
     6721 accgcacagc cacgccgctg cgctgcgggc agctgagttg gccacagaca gaaaagtgga
     6781 agcgctcatg aagggaagcc tgcacaccga cgagatgatc catgccgttc ttgccaaccc
     6841 cgcactgcgc acggcacggc ggatgtccca tgtgttccgg tttgatgtac caacctatgc
     6901 caagccgctc ttgctgacgg acgccgcgtt gaacatccgc cccagtctgg ctgacaaggt
     6961 ggatatcgtg caaaacgcca ttgcctttgc gcgcatcatg ggcacagcgt tgcccaaagt
     7021 ggcggttctg tctgcggtgg aaaccatcaa cccggaaatt ccgtccacac tggatgcggc
     7081 cgccttgtgc aagatggcac accgtggcca gatctctggt ggactgctgg acggtccact
     7141 cgcgtttgac aatgccattt ctgcgcatgc ggcgcgtatc aagcacatcg aatccgatgt
     7201 ggccggagac gcagacatcc tgatggttcc cgacctggag tccgggaaca tgctggggaa
     7261 acagttgcag tacctggccg gcgcgacggc ttccggcatt gtcctgggcg caagagtgcc
     7321 catcgcgctg accagccgcg ctgatggtgt gtctgcacgc atggcctcgg cattgctggc
     7381 caagatgatt gcccaccaca accggatgat tcagccatga ccgtgctggc ggtcaatgcc
     7441 ggttcgtcca cactgaggtt tgcactgcac cccgtggagg gcggcattgc gcagcctgcg
     7501 ctgctgacgg gggtcatcga gggactgtca gcgcaaggcc cactggacat gatctggaat
     7561 gggggaggta caacgcatac cgagcgtagt ggtgacgccg atggtgatgg cttcgagtcg
     7621 gcactggcca tgctgcagaa aatgctccag cgggagtcag ccggacggga gttgcaggcg
     7681 gtagcgcacc gcgtggtcca tggtggggcg cgctaccgtg aggccaccgt tgtcaccgac
     7741 agcgtggtac aggatctgga gcgcctgtgc cggcttgcac ccttgcacca accgcacaat
     7801 gttcagggga ttcgcgcatt cgccaggcgc tttcccactg tgcctcaagt ggcatgtttt
     7861 gacacggcct atcacgccga cctgcccgat gtggagtacc gctttgcctt gccacaagcc
     7921 ctccatgatg aaggtgtgcg gcgctacggc ttccatgggc tttcctacca gtacgtgatg
     7981 cagaggttgg agcaaaccac ctgcatggca cacgggcggg tggtcatggc ccaccttggc
     8041 agtggtgcca gtgtgtgcgg tgccacgcag ggtcagagcc gggcaaccag catgggattc
     8101 tctgcgctcg atggtctcat gatgggaacc cgcgccgggg cactggatgc cggcgtggtg
     8161 ctgcatctgc tggcccaggg gtgggaccac gcccgtttgc agcatctgct gtaccgccag
     8221 agcggtctgc tgggggtatc cgaagtctcg tcggatatgc ggcaactgcg ccgcagtgac
     8281 agcgcgcagg cggacctggc cattcgcatg tttacctacc gcgtggttcg ggaagttggt
     8341 gccatggtgg cctgcattgg cgggctggat gtgctggctt tcaccggagg gatcggtgag
     8401 catgatgcga ccctgcgatc cgaggtgtgc gagcggctgg cgttcctcgg tgtgcggatg
     8461 gatccgcggg ccaatgcact ggcggatggg agcgggccgg ttgcattgca cctgcctgac
     8521 agtgcggtcg aggtgtggat ggttccgacg gacgaggggt ttgtggcggc gcagacagcg
     8581 gtgcgtctgg tgctgtgata ccactggtac cgcgggcatg gggattcccc tgacaaatca
     8641 gatcttgtat ttgtaattaa acggtgtaaa ctgtttatat aattcaaagg aggagtccgg
     8701 tatgcagaca gattttcgcg aatggtctct cggggctgca ctggtgaggt ggtatcaggc
     8761 attgacgcat cactacgcca ttcagacacc cacccacgtc ggagcatttg ccggttcact
     8821 cctggaacgg cgtgactggg atcacaacta gtggcattca tctgtatgtc atggaaatga
     8881 catgaaaaat ccggacactg gagagttgtt gcccgctttt ttatgaccac tcttcctgcc
     8941 gtgacttatg gagccgatgc ctcaggcctg atctgtggct ttcaattcgg tgaggccgga
     9001 ccgggcaccg ccatcggtgc tgaagacgcg ctgcggtggc tggaacagaa cgctgatgcg
     9061 ccgactgcag gatttgtctg gctgcacttc aaccttgcgc acacggcaac cgaaaagtgg
     9121 ctgcgcgaac atgtgcatct gtccaacgcc tttcacgaat ctctgaaaga agggtcccgg
     9181 tcgacccgcg tcgaagtcga cgacgacatg ctggttgcgg tcgtgaatga cgtcgcctat
     9241 gactttgcct tcgagccggg tgacatttcc accatgtggt tgaccgtggg cccgcggctg
     9301 gtgatcagtg cgcgcatgca gcctctgcgg tcgattgacc ggttgcgcga tgcggtcaag
     9361 aagggcatca cgctgcgctc gtccgtggaa ctgctggtgc acctgatgca cgaccaggcg
     9421 acgtgctggt caatattgtg cgcaccacca cagcgcgggt tgacagcctg gaggaccatc
     9481 tgctgtccgg tcg
//
LOCUS       DAOSJM010000042         9490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6941_length_9490_cov_4.742660, whole genome shotgun sequence.
ACCESSION   DAOSJM010000042 DAOSJM010000000
VERSION     DAOSJM010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6941_length_9490_cov_4.742660"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1529)
                     /locus_tag="PKX82_02590"
     CDS             complement(<1..1529)
                     /locus_tag="PKX82_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF51164.1"
                     /translation="MISPPSPPGFNSRLWRRFVTIAGPYWRSDERWRASGMLALLVLL
                     LLGQTAFNVLFNHETGEFTSALAAGDADRFWASIWRYTAILVAAVPIYALYYYVRDTL
                     ALRWRRWLTDSFLARYFSQRAYYRLNTGGIDNPDQRIAEDINSFTQQSLYFSMILLGS
                     LIDLVAFSSVLWTISQGLVYFLIGYAAFSTFFTGSVFGKQLIALNFLQLQREADFRFN
                     LVRVREHAEPIAFHDGEAREMSALQRVFAALYSNYQRVLRWQFKLNLFQYAHRFLIIV
                     LPTAIIANDVLSGELEVGRAIQAAGAFAAILSALTVIVDHFEGLSRFSAGVDRLYAFA
                     ESLGVQAEQAITLPEDDGGQIRTVHGFELATRKLTVLTPNREHLLLKDLTLEVNPGEG
                     LLIVGPSGTGKSSLLRVIAGLWDTGSGEVLRPAGMDMIFLPQQPYLPLGDLRCQLTYP
                     QTERNISDEELLQWLELVNLPTLAQRFGGLNAVLDWPRVLSVGEQQRLAFARALLAKP
                     R"
     gene            1671..3272
                     /locus_tag="PKX82_02595"
     CDS             1671..3272
                     /locus_tag="PKX82_02595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOF51165.1"
                     /translation="MTANRLNPMDAAWIVTESRATPNHVGGLLQFKLPDDAPQDFMRK
                     MMLEFRGHRKFTPPWNQRLKESFNLNPVRTWVEDDNIDLEYHVRHAALPWPGGERELG
                     ELVGRLQSTPIDLTRPPWECTIIEGIAGNRFALFIKIHHSLIDGISGVKLLQRSMSFD
                     RSKSLHLPPFWASGKEHHEQQKNSEPMIPTVTHAIAGAVQELRIQAASVPQLVVAFGK
                     MLKDAMEPKSGMAVPFDAPRSVLNGRVREKRRFATQQFSLERMRTLAAAADCTLNDVV
                     LAVCGGALRRFLSERKSLPKKPLTAGIPVSVRPKDDNGNGNAITFIVSTLGTDIADAR
                     ERLAAIRQSVKRAKEHVQSLPRQAMMQYTVLLMAPTILTLLTGIGGRVRPMFNITISN
                     VPGPETPLYFRGAEMVATYPASIVTHGQALNITCQSYAGNMNFGFTGCHSSMPSMQRL
                     AVYTAEALAELEVAFLSAKPATAARKAAPKAKPAPKPSAAPTLAKTPVKGKAPAKAKP
                     VATAKPKATPTATPVAAKRVRKKAA"
     gene            3297..3965
                     /locus_tag="PKX82_02600"
     CDS             3297..3965
                     /locus_tag="PKX82_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOF51166.1"
                     /translation="MITLYHCVSARSFRPLWLLEELGLPYTLHMLPFPPRALARTYLE
                     ENPLGTVPLLVDGATRMTESAAICEYLAARHSPGALGVAVTEAAYGAYLNWLHMSDAT
                     LTFPQTLVLRYAHFEPAERKQPQVVEDYSRWFLARLRAVDAALQGQEFLCAGRFTAAD
                     VSVAYALQLAGHLGLTDRFSETVQAYWVRLQQRPAFQRSMAVQHQAALDQGVPTTPAP
                     DTRS"
     gene            complement(4026..4436)
                     /locus_tag="PKX82_02605"
     CDS             complement(4026..4436)
                     /locus_tag="PKX82_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOF51167.1"
                     /translation="MKTCAGSSPCPCGRRDGKSRELRYADCCGRYLETDLPAPDAESL
                     MRSRYSAFCLERAPYLQATWHASTRPVGVEFDAGVKWLGLEVRQHMPVDADHAEVEFV
                     ARQREPGGRAVRLHERSRFVREDGRWFYVAGDFE"
     gene            complement(4433..4858)
                     /locus_tag="PKX82_02610"
     CDS             complement(4433..4858)
                     /locus_tag="PKX82_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOF51168.1"
                     /translation="MPITKGFRALVDEAMAQVTTYSVAEVQARLNDPAVQLVDIRDIR
                     ELQDGTVVGAFHAPRGMLEFWVDPASPYHKAIFADEGREFILFCGAGWRSALAAKALQ
                     DMGMTNVAHIDGGWAEWVKQSAPTETLEQQKARRAPKPA"
     gene            4921..7617
                     /locus_tag="PKX82_02615"
     CDS             4921..7617
                     /locus_tag="PKX82_02615"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF51169.1"
                     /translation="MSVRNLDSLFDPASVAVIGASERPASVGATVWRNLLGGGFPGEV
                     YAVNPKHRQLDGHKVYAHIKDLPQTPELAIICTPPATVPGLVHELAKRGTRAAIILTA
                     GLSAEEKRAAQDAARPHLLRLLGPNCIGLLSPHFKLNASFAHIGAQRGELAFVSQSGA
                     LVTAMLDWADAQQIGFSHFVSLGEHMDVDFGDMLDYLATDSRTRAILLYIESIESPRK
                     FMSAARAAARNKPVIVIKSGRSTLGQKAAASHTGALAGSDAVYNAAISRAGMLRVNTL
                     NELFLAAEMLTRCRSTASDALTILTNGGGAGVMAADSAEWIGVPLAKLSPQTVAQLDA
                     VLPPNWSHGNPVDIIGDAPVERYARALEVLAAAPEAGTILFIHAPTAIVPSADIARTL
                     VPMARPKSGPTPRLLSCWLGGHAVADARHQFKQAGIASFETPESAVRGVAMLQAYKRN
                     QTELIEAPPAQSTQVAPDREAVRALVREALANGQTLLTEPQAKAVLDAYGIPVVSTRT
                     VPADPNLAAAEATRIGFPVVIKILSEQISHKSDVGGVALNLDSADEVHEAARVMLSRV
                     KALRPDAVVDGFTVQGMVRRQHVQEVIVGATIDQVFGPIILFGQGGTAVEVMADRAVA
                     LPPLNVPLARALVARTRISKLLKGWRDTPAANEEALLAVLLAVSELLADIPELAELDI
                     NPLLVNHEGAIALDGRIRLSADKPAGALNFAIRPYPSELTETIDWHGRPLEIRPIRPE
                     DEALHTEFLMHLDPEDIRMRVFYSRRTMERSELARLLQIDYTREMAFVAIATGPDGQP
                     QTQGVARAMTDPDNITAEFGIIIRSDIKGGGLGHLLMDKLVAYLRAQGTQQLVATVLR
                     DNSRMLELSHQLGFRDAEVQEDHSTRSIVLDLQSTRTTPQAL"
     gene            complement(7605..8531)
                     /locus_tag="PKX82_02620"
     CDS             complement(7605..8531)
                     /locus_tag="PKX82_02620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF51170.1"
                     /translation="MATAPVVLPAGVSVFERGWLSSNSILIQGAQGNALIDSGYCTHA
                     DQTLALVQSALQGKPLHLLLNTHLHSDHCGGNAALQAAYPALRTSIPPGLADQVLRWD
                     PQALTYEPTGQNCPRFTFDALLLPGTEMPLGDSTWQVHAAPGHDTHSVILFEPVSRTL
                     VSADALWERGFGVVFPELDGDDAFAEVADTLDVIEALRPALVIPGHGSPFTAVDAAIA
                     VARQRLHGFVANPTKHLQYAAKVLLKFKLLELQHVEQAGLLQWVQATPYFKAMFDQLY
                     ADLSLEEGVSRLVQDLVRSGAATVEGGVVYNA"
     gene            complement(8540..>9490)
                     /locus_tag="PKX82_02625"
     CDS             complement(8540..>9490)
                     /locus_tag="PKX82_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF51171.1"
                     /translation="GLDYLTLALVLEEIAAGDGGTSTVISVTNCPVNAILMKFGNPAQ
                     KKQWLEPLAQGQLLGAFCLTEPHVGSDASSLRTTAVREGDEYVINGVKQFITSGKNGD
                     VAIVIAVTDKGAGKKGMSAFLVPTNAPGYVVARLEDKLGQHSSDTAQINFDNCRVPVE
                     NLIGKEGEGYGIALGGLEGGRIGIAAQSVGMARSALDVAIAYSKDRQSFGQPIFNHQA
                     VGFRLAECATQIEAARQLIWHAASLRDAGRPCLKEAAMAKLFASEMAERVCSAAIQTL
                     GGYGYVSDFPVERIYRDVRVCQIYEGTSDVQKIIIQRALG"
BASE COUNT         1780 a         3221 c         2885 g         1604 t
ORIGIN      
        1 tagcggggtt tggccaagag ggcacgggca aaggccagac gctgctgctc ccctaccgac
       61 agcacccggg gccagtcgag caccgcattc agtccgccaa agcgttgggc caacgtcggc
      121 aggttcacca gctccagcca ttgcagcagt tcctcgtccg agatgttgcg ctcggtctgc
      181 gggtaggtaa gctggcagcg cagatcgccc agcggcagat aaggttgctg gggcaggaag
      241 atcatgtcca tgccagccgg gcgcagtact tcgccggagc cggtgtccca caggccggca
      301 atcacccgca gcagcgaact cttgccagtg ccgctggggc cgacgatcag cagcccctca
      361 ccgggattca cctccagcgt caggtccttg agcaacaggt gttcgcggtt gggggtgagg
      421 accgtgagct tgcgtgtggc cagttcgaac ccgtgcaccg tgcggatctg cccgccatcg
      481 tcctccggta acgtgatggc ctgttccgcc tgcacaccca gcgactcggc aaaggcatac
      541 agccggtcga cgcctgccga gaagcggctc agcccttcga agtggtccac gatcaccgtc
      601 agcgcgctca ggatggcggc aaaagcgcct gccgcctgga tggcccggcc cacttccagc
      661 tcgcccgaga gcacgtcatt ggcaatgatc gcggtgggca gcacgatgat cagaaaccgg
      721 tgcgcatact ggaacaggtt gagcttgaac tgccagcgca gaacgcgctg gtaattgctg
      781 tacagcgccg cgaacacacg ctgcaacgcc gacatttcgc gcgcttcgcc atcgtggaag
      841 gcaataggct cggcatgttc gcgcacccgc accaggttga aacggaaatc cgcctcccgc
      901 tgcaactgca gaaaattcag cgcaatcagc tgcttgccaa acaccgaccc ggtaaaaaag
      961 gtgctgaatg cagcgtagcc aatcaggaag tacaccagcc cctgcgagat cgtccacagc
     1021 acgctggaaa atgcgaccag gtcaatcagc gagcccagca gaatcattga gaaatacagc
     1081 gactgctgcg tgaagctgtt gatgtcctcc gcaatccgct ggtccgggtt gtcgatgccg
     1141 ccggtgttga gccggtagta agcgcgctgg ctgaaatagc gcgccagaaa actgtccgtc
     1201 aaccagcggc gccagcgcag cgccagcgta tcgcgcacgt agtagtacag cgcatagata
     1261 ggcaccgcag ccaccaggat ggccgtgtag cgccagatcg acgcccagaa acggtctgca
     1321 tcgccggccg ccagggccga agtgaattcg ccagtctcgt ggttgaacag cacgttaaac
     1381 gccgtctgcc ccagcagcag cagcaccagc agggccagca tgccgctggc gcgccagcgc
     1441 tcgtccgagc gccagtaggg gccggcaatc gtgacaaaac gccgccacag gcgggagttg
     1501 aaaccggggg gagatggagg actgatcatg gtgtggcgcg caatcagggg gcactctagc
     1561 acgcgccccc agcagcaacg cacccaggct aggtgtaaac catcagtcgg gcgcaggctg
     1621 gcaggtgctc taatctcact attcaaaaca cacatgccct gcaccgtttt atgaccgcca
     1681 atcgcctcaa tcccatggac gccgcctgga tcgttaccga atcccgcgcc acccccaacc
     1741 acgtcggtgg tctgctgcag ttcaagttgc ccgacgacgc cccccaggat ttcatgcgca
     1801 agatgatgct ggagtttcgc gggcaccgca aattcacccc gccctggaac cagcgcctca
     1861 aggagtcgtt caacctgaac ccggtgcgta cctgggtgga ggacgacaac atcgacctcg
     1921 agtaccacgt gcgccacgcc gccctgccct ggcccggcgg cgaacgcgaa ctcggcgagc
     1981 tggtcggccg cctgcagagc acgccgatcg acctgacccg cccgccgtgg gaatgcacca
     2041 tcatcgaagg cattgcgggc aaccgattcg cgctgttcat caagatccac cattcgctga
     2101 tcgatggcat cagtggcgta aaactgctac agcgcagcat gtccttcgac cgcagcaaga
     2161 gcctgcacct gccaccgttc tgggcttcgg gcaaagaaca ccacgagcag caaaaaaatt
     2221 ccgagcccat gatcccgacc gtcacccatg cgattgccgg ggcggtgcag gagttgcgca
     2281 ttcaggctgc ctccgtgccg caactggtgg tggccttcgg aaagatgctg aaggatgcga
     2341 tggaacccaa gagcggtatg gcggtgccgt ttgacgcgcc acgctcggtg ctcaatggcc
     2401 gggtccgcga aaagcggcgt tttgccaccc agcagttctc gctggagcgc atgcggacgc
     2461 tggccgctgc ggccgactgc acgctcaacg acgtggtact ggccgtctgt ggcggtgccc
     2521 tgcgacgctt cctgagcgaa cgcaagagcc tgcccaaaaa gccgctgacc gccggcattc
     2581 cggtgtccgt acgtcccaag gacgacaatg gcaacggcaa cgccattacg ttcatcgtgt
     2641 ccacgctggg caccgatatt gccgatgcgc gtgagcgtct cgcagccatc cgccagtcgg
     2701 tgaaacgtgc caaggagcat gtgcaaagcc tgccccgcca ggccatgatg cagtacaccg
     2761 tgctcctgat ggcacccacc atcctgacct tgctgaccgg cattggcggg cgtgtacggc
     2821 ccatgttcaa catcaccatc tccaatgtgc cgggaccaga gacaccgctg tatttccgtg
     2881 gtgctgaaat ggtggcgacc taccccgctt ccatcgtcac gcatggccag gccctgaaca
     2941 tcacctgcca gagctacgcc ggcaacatga acttcggctt cactggctgc cactcgtcca
     3001 tgccgagcat gcagcgcctg gcggtctaca ccgcagaagc cctggccgag ctcgaagtcg
     3061 cgttcctgtc ggccaaaccg gccactgcgg cgcgcaaggc ggcgcccaaa gccaagccag
     3121 ccccgaaacc cagcgcagcc cccaccctgg ccaagacacc ggtaaaaggc aaggcacccg
     3181 ccaaagccaa acccgtggcc acggccaagc ccaaggcaac tccgactgcg acgccggtgg
     3241 cggccaagcg cgtgcgcaaa aaagccgcct agccgcgacg ctttagactg cggcgcatga
     3301 tcaccctgta ccactgcgtc agcgcccgct ccttccgccc cctgtggctg ctggaagagc
     3361 tgggcttgcc ctacaccctg cacatgctgc cattcccacc ccgggccctg gcacgcacct
     3421 acctcgaaga aaacccactg ggtaccgtgc cgctgctggt cgacggggcc acccgcatga
     3481 ccgaatcggc cgccatctgc gagtaccttg ctgcccggca cagccccggt gcgctggggg
     3541 tggcggtgac ggaagccgcc tacggcgcct acctgaactg gctgcacatg agcgacgcca
     3601 ccctgacctt cccgcagacg ctggtgctgc gctacgccca ctttgagccc gcggaacgca
     3661 agcagccgca ggtggtggaa gactacagcc gctggtttct ggcccgcctg cgcgccgtgg
     3721 atgcggcgct acaggggcag gagtttctgt gtgcggggcg gtttacggcg gcagacgtca
     3781 gcgtggccta tgcgctgcag ctcgccggcc atctggggct gaccgatcgc ttttccgaaa
     3841 ccgtccaagc ctattgggtc cggctgcagc agcgcccggc ctttcagcgc agcatggcgg
     3901 tccagcacca ggcggcgctg gaccagggcg tgccgacgac ccctgcgcca gacacccggt
     3961 cctgacaata tttgctatta catttatagc tactcacgca cattccacgg gggctggagc
     4021 ccgatttatt caaaatcccc agccacatag aaccagcgac cgtcttcccg cacaaaacgg
     4081 ctgcgttcgt gcaggcgcac ggcacgccca ccgggctcgc gctggcgcgc cacgaattcg
     4141 acctcggcat ggtcagcgtc caccggcatg tgttggcgca cttccagccc cagccatttc
     4201 acgccagcat cgaattcaac gcccaccggg cgcgtgctgg cgtgccaggt ggcctgcaga
     4261 tacggtgcac gttccaggca gaacgcgctg tagcgggagc gcatgaggct ctcggcatcc
     4321 ggcgccggca ggtcggtttc caggtagcga ccacagcagt ccgcgtaacg cagttccctg
     4381 gacttgccat cgcgccgccc gcacgggcac gggctgcttc cggcgcaagt cttcatgccg
     4441 gtttgggcgc gcgacgggcc ttttgctgct ccagtgtctc ggtcggagca ctttgcttca
     4501 cccattcggc ccaaccgcca tcgatatgcg ccacattggt catgcccatg tcctgcagcg
     4561 ccttggccgc cagcgcactg cgccaacccg ccccacagaa caggatgaac tcccggcctt
     4621 cgtcggcaaa aatcgccttg tggtacggcg atgccgggtc cacccagaat tccagcatgc
     4681 cgcggggcgc atggaaggcg ccgaccacgg tgccgtcctg cagctcgcgg atgtcgcgga
     4741 tgtccacgag ctgcacagca gggtcattca agcgggcctg cacctcggcc acgctgtagg
     4801 tggtgacctg cgccatggct tcgtccacaa gcgcgcgaaa tcctttggta atcggcatgg
     4861 gttttctttc acattccaga attagacttg cagcatagct aatctccaaa ggcactgtcc
     4921 atgagcgtac gcaatcttga ctccctgttc gaccccgcct cggtggcggt gattggcgcg
     4981 tccgagcggc ccgccagcgt gggggccaca gtgtggcgca acctgctggg tggtggtttt
     5041 cccggtgagg tctacgcagt gaaccccaaa caccggcagc tcgacgggca caaggtttat
     5101 gcccacatca aggacttgcc ccagacgcct gagctggcca ttatctgcac cccaccggct
     5161 acggtgcccg gtctggtgca cgagttggca aagcggggca cccgcgcggc catcatcctc
     5221 acggccggtt tgagtgccga agaaaagcgt gccgcgcagg atgccgcccg gccccacctg
     5281 ctgcgcctgc tgggccccaa ctgcattggc ctgctgtcac cccacttcaa gctcaatgcc
     5341 agctttgccc acatcggtgc acagcggggc gagctggcgt ttgtgtccca gtccggcgcg
     5401 ctggtcaccg ccatgctcga ctgggccgac gcgcagcaga tcgggttttc gcactttgtc
     5461 tcgctgggcg aacacatgga tgtggacttc ggcgacatgc tcgattacct ggccaccgac
     5521 agccgcaccc gcgccatcct gctctacatc gagtccatcg aatccccccg caagttcatg
     5581 tcggccgcgc gcgcggccgc gcgcaacaaa ccggtcatcg tgatcaagtc gggccgctcc
     5641 acgctggggc aaaaagccgc agcctcgcac acgggcgcat tggcgggctc cgacgcggtg
     5701 tacaacgcgg ccatttcccg cgccggcatg ctgcgcgtca atacacttaa cgaactgttc
     5761 ctggcggccg agatgctgac ccgctgccgg tccacggcca gcgacgccct gaccattctg
     5821 accaacggcg gtggcgccgg tgtgatggcc gccgactcgg ccgaatggat tggcgtaccc
     5881 ctggccaaac tgtcaccgca aacggtggcc cagctcgacg ccgtgttgcc gcccaactgg
     5941 tcccacggca acccggtcga catcattggc gatgctccgg tggaacgcta tgcccgcgca
     6001 ctggaggtgc tggcggctgc gcccgaggcc ggcaccatcc tgttcatcca cgcacccacc
     6061 gccatcgtgc ccagcgcgga cattgcgcgc accctggtgc cgatggcccg tccgaagtcg
     6121 ggcccaacgc cacggctgct gagctgctgg ctcggtggcc atgcagtggc tgatgcgcgc
     6181 caccagttca agcaggccgg cattgccagc ttcgagacgc cggagtcggc ggtgcgcggt
     6241 gtggccatgc tgcaggcata caaacgcaac cagaccgaac tcattgaggc gccgcccgcg
     6301 caatccacgc aggtcgcacc cgaccgcgag gcggtacgcg ccctcgtgcg tgaggcgctg
     6361 gccaatggac agaccctgct gaccgagcct caggccaaag ccgtgctcga cgcctacggc
     6421 attcccgtgg tgtccacccg caccgtgcct gcagacccga acctggccgc tgccgaggcc
     6481 acgcgcatcg gttttccggt ggtgatcaaa atcttgtcgg aacagatttc ccacaagtcc
     6541 gacgtgggcg gtgtggccct gaacctcgac agtgcggacg aagtacacga agcggcgcgc
     6601 gtgatgctgt cgcgcgtcaa ggcattgcgt cccgatgcgg tggtggatgg cttcacagta
     6661 caaggcatgg tgcggcgcca gcatgtgcag gaggtgatcg tcggcgccac catcgaccag
     6721 gtgttcggtc ccatcatcct gtttggccag ggcggtaccg ctgtggaggt gatggccgac
     6781 cgcgccgtgg cactgccccc gctgaacgtt ccgctggcgc gcgcgctggt ggcccgcacc
     6841 cgcatctcca aactgctcaa gggttggcgc gacacgcccg ctgcgaatga ggaggcgctg
     6901 ctggccgtgc tgctggcggt gtccgagctg ctggccgaca ttcccgagct ggcagagctg
     6961 gacatcaacc cgctgctggt caaccacgaa ggggcgatag ccctggatgg acgcatccgg
     7021 ctgagcgcgg acaaacctgc tggcgcgctg aattttgcga tccggcccta tccgtccgag
     7081 ctgacagaga ccatcgactg gcacggccgt ccgctggaaa tccgcccgat ccgtccggaa
     7141 gacgaggcgt tgcacaccga gttcctgatg cacctcgatc cggaagacat ccgcatgcgt
     7201 gtgttctaca gccgccgcac catggagcgc agcgaactcg cgcgcctgct ccagatcgac
     7261 tacacccgtg agatggcgtt tgtggccatc gccaccggac ccgatggcca gccgcagacc
     7321 cagggcgtgg cgcgggccat gaccgacccg gacaacatta ccgcggaatt cggcatcatc
     7381 atccgttcgg acatcaaggg cggcgggctg ggccatctgc tgatggacaa gctggttgcc
     7441 tacctgcgcg cgcagggcac gcagcagctg gtggccaccg tgctgcgcga caacagccgc
     7501 atgctggagc tgtcacacca gctcggcttc cgggatgctg aggtgcagga agaccacagc
     7561 accaggtcca tcgtgctgga tctgcaatcc acccgtacaa cgcctcaggc gttgtagacc
     7621 acgccgccct cgacggttgc cgcaccggaa cgcaccagat cctgtaccaa ccggctcacc
     7681 ccttcttcca gagagaggtc ggcatacagc tggtcaaaca tggctttgaa atagggcgtg
     7741 gcctgcaccc attgcagcag cccagcctgc tccacgtgct gcaattccag cagcttgaac
     7801 ttgagtaaca ccttggcagc gtattgcagg tgtttggtcg ggttggccac aaagccatgg
     7861 agccgctggc gcgccactgc aatcgcagca tccactgccg taaagggtga accgtgcccg
     7921 gggatgacca gcgcaggccg caaggcctca atcacgtcca gcgtgtcggc cacttcagca
     7981 aaggcatcgt cgccatccag ttccggaaac accacgccaa agccacgctc ccagagcgca
     8041 tcggccgaca ccagcgtgcg gctgactggc tcgaacagga tgaccgaatg cgtgtcgtgg
     8101 cccggcgcgg catggacctg ccaggtgcta tcgcccaacg gcatctcggt acccggcaac
     8161 agcagtgcat cgaaggtaaa ccgcgggcag ttctgccctg tgggctcata cgtcaaggcc
     8221 tgcgggtccc agcgcagaac ctggtccgcc agccccggtg gaatggaggt gcgcagtgcg
     8281 gggtaagccg cctgcagtgc cgcattgcca ccacagtgat cactgtgcaa atgggtgttg
     8341 agcagcaggt gcaggggctt cccctgcagg gcggattgca ccagcgccag cgtctggtcc
     8401 gcgtgggtgc agtagccgct gtcaatcagg gcattgccct gcgcaccctg gatcaggatg
     8461 ctgttgctcg acaaccagcc gcgctcgaat acggaaacac cggcaggcag gaccacgggc
     8521 gccgttgcca tgctgtcgct cagcccagcg cgcgctggat gatgatcttc tgcacgtcgg
     8581 acgtgccttc gtaaatctgg cacacgcgca catcgcggta gatgcgctcc accgggaagt
     8641 cgctcacata gccgtagccg cccagcgtct ggatggcggc gctgcacacg cgctcggcca
     8701 tctcgctggc aaacagcttg gccatggccg cttctttcag gcagggccgg cccgcatcgc
     8761 gcaacgaagc ggcatgccat atgagctggc gcgccgcttc gatctgcgtg gcgcattcgg
     8821 ccagacggaa gcccacagcc tggtggttga agatgggttg gccaaaactc tggcggtctt
     8881 tggagtaggc aatcgccaca tccagcgcgc tgcgggccat gcccacgctt tgcgccgcaa
     8941 tgccgatgcg tccgccttcc aaaccaccca gggcgatgcc gtagccttcg ccctctttgc
     9001 caatcaggtt ctctacgggc acgcggcagt tatcgaaatt gatttgcgcg gtgtcgctgg
     9061 aatgctggcc gagcttgtct tccagacgtg ccaccacata gcccggtgcg ttggtcggca
     9121 ccaggaaggc gctcataccc ttcttgccgg cgcccttgtc ggtgacggca atcacgatgg
     9181 ccacatcgcc attcttgccg ctggtgatga actgcttgac gccgttgatg acgtattcat
     9241 ccccttcgcg cactgccgtg gtccgcaggc tggaggcatc actgcccaca tgcggctcgg
     9301 tcaggcagaa cgcccccagc agctgaccct gcgccagcgg ttcaagccac tgtttcttct
     9361 gcgccgggtt gccaaacttc atgagaatgg cgttgaccgg gcagttggtc acgctgatca
     9421 cggtgctggt accgccgtca ccggcggcaa tttcttccag caccagcgcc agcgtcaggt
     9481 agtccagccc
//
LOCUS       DAOSJM010000043         9425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_6986_length_9425_cov_4.378655, whole genome shotgun sequence.
ACCESSION   DAOSJM010000043 DAOSJM010000000
VERSION     DAOSJM010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9425
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6986_length_9425_cov_4.378655"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..169
                     /locus_tag="PKX82_02630"
     CDS             <1..169
                     /locus_tag="PKX82_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HOF51172.1"
                     /translation="GDQFTVADGYLFVVTNWAPFVGLDLSPYPNLVAYRARVGARPAV
                     IAAMKAEGLVK"
     gene            174..596
                     /locus_tag="PKX82_02635"
     CDS             174..596
                     /locus_tag="PKX82_02635"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HOF51173.1"
                     /translation="MSAEMRTAIDPTRDAFKQLFAHAPAQGPVQMLNLLAFRERTEDG
                     LRTGRRAYADYGRAVAPILAGVGGRVIWAANAHLAFIAPPGEHWDEALLVEYPSRDAF
                     VEMLQSPAYQAIVHHRTAALRDARLIVTTPLPANLPSG"
     gene            836..1060
                     /locus_tag="PKX82_02640"
     CDS             836..1060
                     /locus_tag="PKX82_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51174.1"
                     /translation="MKDRALWPVPMEIVGVVGWYLMRRGQIQPAIASLAVGMWMCVAV
                     MVVVLAPRSSTSSRSSSSCWGGSPTRKRPS"
     gene            1021..3345
                     /locus_tag="PKX82_02645"
     CDS             1021..3345
                     /locus_tag="PKX82_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF51175.1"
                     /translation="MLGWLTHTKAAIVTAALTSIFTIGVIWAGAYGGLPRAPESPPAL
                     QGLVQICVFATAALLVHSLVQAYQRRLQELNAINHDFSQRTRDLERTKSELHQAQAVA
                     RVGSWVYDLTTDTVYPSDETCRILGIPLGSTLNQKQYVAHTHPEDRQNLLHTMQSVLE
                     NKSAFDYEHRIVVGDAIRWIRQKAEFGPSTVGNSTRILGITQDINDRKIAELSLQQSE
                     KKFSTAFKSSPVAASIATLREGRFVEANDKYARDFGWTRTELIGHTIQDIGLWPSPEG
                     RKRWVKTLMERGSIVDYETTWIHKNGSQRNVSISGEIIDYDGTPCILGFVTDISERKA
                     AEEKIQNLAFYDSLTGLPNRRLLLDRLELALAKTHRNQLHGGLLFIDLDNFKTLNDTH
                     GHNKGDQLLQQVASRLGACVREGDTVSRLGGDEFVVMLDNLSGNALVAASQAEAVSEK
                     ILGTLDQNYRIGDIHFHNTASIGITLFGDQLERLEEPLKRADTAMYQAKSAGRNTIRF
                     FDPDMQAAITALVQLESELRTAVQQHQFLLHYQPQIDATGKVTGVEALVRWQHPGRGL
                     VPPVEFIRLAEETNLILTLGTWILETACSQLARWAKRPGFASLTISVNVSARQFQQVD
                     FVEQVLATLEHTGANPRLLKLELTESMLIDNVASVIEKMTALKAHGITFSLDDFGTGY
                     SSLSYLKKLPLAQLKIDRSFVRDVLLDPSDAAIARMVIVLAQTLGLNVIAEGVETAAQ
                     MEFLSQRGCQNFQGYYISHPLHIDPLENFVQSRL"
     gene            complement(3342..4538)
                     /locus_tag="PKX82_02650"
     CDS             complement(3342..4538)
                     /locus_tag="PKX82_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HOF51176.1"
                     /translation="MGTLLQTPLRQLLWLAAALSMGAAVSLGITRFAYGLLLPAMRSD
                     LGWTYTLAGGMNTANALGYFLGALATPWLMRRLGPSTLLWTGALLASSFMALSGFFVE
                     ATPLLVQRLLAGVASALVFIAGGLLAARLGAQDPQRGGLLLGMYYGGTGVGIVLSAVL
                     VPWVLDIAQAQAHGWRWAWWALALLCVLASLLLLWPARVMGQWAQPAAAAQAAHMEFR
                     WHPFRYGLAGYTLFGVGYIGYMTFVVALLRAQGSSTLAVTVFYALLGLAVMASSRIWA
                     ALLDRHKGGRSMATLNALLGLATVLPALTQSWPVVLASGLLFGGVFLSVVASTTALVR
                     HNLPPSAWAAGISAFTTVFAAGQIIGPTVVGWIADGPGGLARGLVFSAFALWLGALLA
                     LKQKAL"
     gene            4610..5725
                     /locus_tag="PKX82_02655"
     CDS             4610..5725
                     /locus_tag="PKX82_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HOF51177.1"
                     /translation="MTTLFSPLQIGSLTLANRVVMAPLTRNRAPGALPTALMATYYTQ
                     RADPKNGAGLIVSEATAISHQGQGYSDVPGIWSDAQVEAWKPVTAAVHAAGGKIFMQL
                     WHVGRVSHVDLQPGGQPPVAPSALTAKTKTVLIQNGVPKFVDTSAPRALDIAELPGIV
                     QDYRRAARNAVAAGFDGVEVHGANGYLLDQFLRAGSNQRTDTYGGSIENRARLLVEVM
                     QAVCDEIGGDKVGLRISPVTPANDAADPQPQPLFEHVVRQLAPMKLAYLHVIEGSTGG
                     PRDYVQGDKAFDYVALRNAYRAAGGAAAWMVNNAYDRALADQALDNGADLVAFGRPFI
                     ANPDLTRRLREGAALNAGDRMTYYGGGAAGYTDYPTL"
     gene            complement(5771..6355)
                     /locus_tag="PKX82_02660"
     CDS             complement(5771..6355)
                     /locus_tag="PKX82_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOF51178.1"
                     /translation="MLRQSLQIIQEEHTALAAMLRSLSMMVDRGPDDEPERYFDVLRA
                     MLFYVDEFPEKLHHTKESNLLFPRVARLAPETQEAIARLEKDHAMSEAGVRELQHLLL
                     AWELVGDSRRVAFEEATRRYIAAYLEHMHLEESAILPIARKVLSDEDWKELDTAFATN
                     CDPLTGKYPRDPMYDRLFTRIVMRAPAPIGLGDA"
     gene            complement(6428..8029)
                     /locus_tag="PKX82_02665"
     CDS             complement(6428..8029)
                     /locus_tag="PKX82_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOF51179.1"
                     /translation="MLTLMRLFTIRFRMLSAIAVVLGLLSILGGVGLWSMFRMQALSQ
                     DFMDHTFADAGRLSRLQFELGRTRVAEKEMVIHYESATAVKDAHGRWQISVKNALEQA
                     QALSSAGSQTVARAAQTARTHIDGYAAAMAPVVTQLSGGAVPSATVGNSLLTKANQEF
                     AELEKQLLALEDLVQKQAGAAVKEQEDASGTSKLLFALALVLTIVVVGPLTWLNMLSI
                     CKPVEQARGFAQAITSGDLSVDIRVEGSDELSDLMRSLDGMRDSLANTVGHMRDASEN
                     IASASQEIASGNQDLSVRTERAASNVQSMVSSISELLGTVQQTASSAQTANQLASAAS
                     GQASRGGEVVTHVVTSMHEISGASRKIGDIIGLIDSIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVASEVRSLAQRSAQAANDVKHLIQASVTAVDGGVRLAEEAGTAMQEMVSGAQ
                     RVGGIIGEISSASVEQSQGISSVNQTVTEIDQMTQQNAALVEQSAAAAESLREQAERL
                     AQLVRQFRLREGAVHAGQSLRIGSA"
     gene            complement(8057..>9425)
                     /locus_tag="PKX82_02670"
     CDS             complement(8057..>9425)
                     /locus_tag="PKX82_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOF51180.1"
                     /translation="EPGGKLTGGSINGDGRVVMQVRAVGSETVLSHIIRLVEDAQAAK
                     APIQRLVDQVSAVFVPVVLVIALVTLLAWWLTGHSFEQSLIYCVAVLVIACPCALGLA
                     TPAAIMAGTGVAAKYGILIKDAQALELAHKVDVVAFDKTGTLTVGRPVLTRLVPTQGS
                     DEAQVLALVASLQSGSEHPLARAVMDAARERGVAFEAPDNVRAVPGRGTEGEVGVRSF
                     LLGSLRWMEELGVHLEALAEQASALQSEGATVSAFVERSTGGLRPLALLAFGDEPKPG
                     AKAALAALRARGIKTVMISGDNRGAAEAMARRLGLDPQAGEVMAEVLPGDKAAHVVAL
                     KQGGHVVAMVGDGVNDAPALTAADVGMAMANPGGGGTDVAMHAAGITLMRGDPLLVAA
                     ALDISDRTVSKIRQNLFWAFAYNAAGIPLAALGYLNPVLAGAAMAMSSVSVMSNALLL
                     KRWKP"
BASE COUNT         1908 a         3211 c         2679 g         1627 t
ORIGIN      
        1 gggcgaccag ttcaccgtgg ccgatggcta cctcttcgtg gtcaccaact gggcaccatt
       61 tgtcggtctg gacctgagcc cctatcccaa tctcgtggcc taccgggcgc gcgtgggtgc
      121 acgtcctgca gtcattgccg ccatgaaggc tgaaggtctg gtcaagtagc gccatgtctg
      181 ccgaaatgcg caccgccatt gaccccacac gggacgcttt caaacagctg ttcgcgcacg
      241 ctccggcaca gggcccggtg caaatgctca accttctggc gtttcgtgag cgtaccgaag
      301 atggcttgcg cacgggccgc cgggcctatg ccgactacgg tcgggctgtg gcacccatcc
      361 tcgctggcgt aggtggtcgg gttatctggg ctgcgaatgc acacctggcc ttcattgctc
      421 ctcccggtga acactgggat gaagcgctgc tggtggaata tccgtcacgc gatgcttttg
      481 tggaaatgct gcaatctcct gcctaccagg ccatcgtgca ccaccgcacg gcggctttgc
      541 gggatgcacg cctgattgtc acgaccccgc tacctgccaa cttgccgtcg ggataaacgc
      601 accggggtat tgcgctacca tgggcgaaag gaacgaaaga agtcggggat atccatccac
      661 aggctgacag gagaatgtca tcgcgacgct gccacccccc acgcaccaga atgacgaaga
      721 ggtctacaac ccacatacct gggagccgtc ggttgcgttc ctgaaatacg ccatctgggc
      781 cattctgctg agcgtcgctg cggtcgttct cgcgatctat ttctttgttc ccaatatgaa
      841 agaccgcgcc ctgtggccgg tgcccatgga aatcgtcggc gttgtgggct ggtacctgat
      901 gcgtcgcgga cagatccagc ccgccatcgc cagcctggca gtgggcatgt ggatgtgtgt
      961 ggctgtgatg gtggtggtgt tagcgccccg gtccagtaca tcttcccgct catcatcttc
     1021 atgctggggt ggctcaccca cacgaaagcg gccatcgtga ctgcagcgtt gacatcgata
     1081 ttcaccattg gtgtgatctg ggccggtgcc tatggtggcc tgcccagggc accggagtct
     1141 ccaccggcgc tccagggcct ggttcaaatc tgcgtctttg ccacggcggc ccttttggtg
     1201 cattccctgg tgcaggccta ccagcgacgg ctgcaggaat tgaatgccat caaccacgat
     1261 ttctcccaac gcacacgtga tcttgaacgc accaaatccg agttgcacca ggcccaggct
     1321 gtggccaggg tgggcagctg ggtgtatgac ctgacgacag acacggtgta cccctcggat
     1381 gaaacctgcc ggattctggg tattccactt gggagcaccc tcaaccagaa acagtacgtc
     1441 gcacacaccc acccggaaga ccgccagaac ctgctgcaca ccatgcagtc ggttctggaa
     1501 aacaaaagcg cattcgatta cgaacaccgg attgtggtgg gtgacgccat ccgctggata
     1561 cgccaaaagg ccgaattcgg cccctccacc gtgggaaaca gcacccgcat actcggcatt
     1621 acgcaggaca tcaacgacag aaaaatcgcg gaactgagcc tgcagcaatc cgagaaaaag
     1681 ttctctactg cgttcaaatc cagtccggtc gcggcctcca ttgccaccct gcgagaaggc
     1741 cgctttgtcg aggccaatga caagtacgca cgggacttcg gctggacacg caccgaactc
     1801 attggccaca caatacagga catcggactg tggcccagcc cggagggacg caagcgctgg
     1861 gtaaagaccc tgatggaacg cggcagcatc gttgactacg aaaccacgtg gatccacaag
     1921 aacgggagcc agcgcaatgt cagcatatcg ggggaaatca ttgactacga cggtacgccc
     1981 tgcatcctgg gtttcgtcac agacatctcc gaacgcaaag cagcagaaga gaaaatccag
     2041 aacctggcct tctacgactc cctgaccgga ttgcccaacc gacgcctgct gctcgaccgt
     2101 ctggaactgg ccctggccaa gacccaccgc aaccagctgc acggcggcct gctgttcatc
     2161 gacctcgaca acttcaagac cctcaacgac acgcacggac acaacaaggg cgaccagctc
     2221 ctgcaacagg tggccagccg tctaggcgcg tgtgtccgcg aaggagacac tgtgtcacgt
     2281 ctgggtggag acgagtttgt cgtcatgctc gacaacctca gtggaaacgc gctggtggcc
     2341 gccagccagg cggaagccgt atccgaaaag attctgggca cgctggacca gaactaccgg
     2401 atcggcgaca tccactttca caacacggcc agcatcggta tcaccctgtt cggcgaccag
     2461 ctggaacgac tcgaagagcc gctcaaacgt gccgacacgg ccatgtacca ggccaagtcg
     2521 gccggacgca acaccatccg cttttttgat cctgacatgc aggccgccat taccgccctg
     2581 gtgcagctgg agtccgagtt gcgtactgcc gtccagcaac accagttcct gctgcactac
     2641 cagccccaga tcgatgccac cggcaaggtg accggtgtag aggcactggt ccgctggcaa
     2701 caccccgggc gcggtctggt accaccggtt gagttcattc gcctcgccga agaaaccaac
     2761 ctcatcctca ccctgggaac ctggattctg gaaacggcct gcagccagct ggcccgctgg
     2821 gcaaagcgtc ccgggttcgc gtccctgacc atctccgtca acgtcagtgc ccgccagttc
     2881 cagcaggtgg actttgttga acaggtactg gcgactctgg agcacaccgg tgccaacccc
     2941 cgactcctca aactggagct gacggaaagc atgctgatcg acaacgtggc gtccgtcatt
     3001 gaaaagatga ccgcactcaa agcgcatggc atcacctttt cgctggacga tttcggcacc
     3061 ggctactcct ccctttccta cctcaagaaa ctgcccctgg cccaactcaa gatcgatcgc
     3121 agttttgtgc gtgatgtact gctggacccg agtgatgcag ccattgcgcg catggtgatc
     3181 gttctggcgc agaccctggg gctcaacgtc atcgccgaag gggtggaaac ggccgcccag
     3241 atggagttcc tgtcacagag gggctgccag aacttccagg gctactacat cagccatcca
     3301 ctgcacatcg acccgctgga aaacttcgtg caatcccgcc tttaaagtgc tttctgcttc
     3361 aacgccagca aggcacccaa ccacaaggca aaggcggaaa agaccagccc acgcgccagc
     3421 ccgccggggc cgtcggcaat ccagcccacc acggtcgggc caatgatctg cccggctgca
     3481 aatacggtgg tgaatgcact gatccccgca gcccaggcac tgggtggcaa gttgtggcgc
     3541 accagtgccg tagtggacgc caccaccgac agaaacaccc cgccaaacaa cagccccgat
     3601 gccagcacca cgggccagga ctgggtcagc gcgggtaata ccgtagccag ccccagcaag
     3661 gcgttcaacg tagccatgga ccgcccgccc ttgtggcggt ccagcagggc agcccagatg
     3721 cgactggacg ccatgaccgc cagccccagc agggcataaa acacggtcac cgccagcgtg
     3781 ctgctgccct gtgcgcgcaa cagcgccacc acaaaggtca tgtagccgat gtaacccacg
     3841 ccaaacaggg tgtaacccgc caacccgtag cgaaacgggt gccagcgaaa ctccatgtgg
     3901 gccgcctgcg cagccgccgc cggctgtgcc cactggccca tcacccgggc cggccacagc
     3961 aacagcaggc tggccagcac acacagcaga gccagagccc accaggccca gcgccagccg
     4021 tgggcctgcg cctgggcaat gtccagcacc cagggcacca gcaccgccga aagcacaatg
     4081 cccacaccgg tgccaccgta atacatcccc agcaacaggc cgccacgctg cgggtcctgc
     4141 gccccgagcc gggccgccag caaaccgcca gcgatgaaca ccagtgcgct ggccacaccc
     4201 gccagcaagc gctgcaccag cagcggtgtg gcttccacaa aaaatccact gagcgccatg
     4261 aagctactgg ccagcaacgc tccggtccac agcaaagtgg aagggcccag acggcgcatc
     4321 agccatggcg tggccagggc ccccagaaaa taccccagcg cattggccgt gttcatgcca
     4381 cccgccagcg tgtaggtcca gcccagatcc gagcgcatgg ctggcagcag caaaccgtag
     4441 gcaaagcggg tgatccccag cgacacggcc gcacccatgg aaagcgcggc tgccagccat
     4501 aacagttgtc gcaaaggtgt ctgcaaaagc gtccccatcc cgtacattgg agcttgcatt
     4561 ttgctgcaac gacagcacct gtgtacttcc cctatccaag agcttttgca tgaccacttt
     4621 gttctccccc ctccagatcg gcagcctcac cctggccaac cgcgttgtca tggccccgct
     4681 gacgcgcaac cgtgcgcccg gtgccctgcc cactgccctg atggcaacct actacaccca
     4741 gcgggccgac ccgaagaatg gtgcgggcct gatcgtctcg gaggccaccg ccatcagcca
     4801 ccagggccag ggctattcgg acgtacccgg catctggagc gatgcccagg tcgaggcctg
     4861 gaagcctgtg actgccgcag tgcatgcggc gggcggcaag atcttcatgc aactctggca
     4921 tgtaggccgt gtctcgcacg tcgacctgca gcccggtggc cagcccccgg tcgcgccgtc
     4981 agcactgacg gccaagacca agaccgtact gattcagaac ggtgtgccca aattcgttga
     5041 cacctccgca ccgcgtgccc tcgacatcgc tgaattgccc ggcatcgtgc aggactaccg
     5101 ccgcgccgca cgcaatgccg ttgctgcggg ttttgatggg gtggaagtcc acggggctaa
     5161 cggctacctg ctcgaccagt tcctgcgcgc gggctctaac cagcgcaccg acacctacgg
     5221 cggcagcatc gaaaaccgcg cgcgtttgct ggtcgaggtc atgcaagccg tctgcgatga
     5281 aatcggtggt gacaaggtgg gcctgcgcat ttcacccgtc accccggcca acgacgcggc
     5341 cgatccacaa ccccagccac tgttcgagca cgtggtccgg caactggcgc ccatgaagct
     5401 ggcttacctg catgtgattg agggctccac cggtgggcca cgcgactatg tgcagggcga
     5461 caaagccttc gattacgttg ccttgcgtaa tgcgtatcgc gccgcgggcg gtgccgcagc
     5521 ctggatggta aacaacgcct atgaccgcgc gctggccgat caggcgctgg acaacggtgc
     5581 cgacctggtg gcgtttggcc ggcccttcat tgccaacccg gacctcacgc gccgcctgcg
     5641 cgaaggggct gcgctcaatg caggcgaccg catgacgtac tacggcggtg gtgcagccgg
     5701 ctacaccgac tacccaacgc tataatttta gaagcagcta acgtccggtg gacgagggct
     5761 gcaagccaat tcaggcatca cccagaccaa tgggcgcggg tgcccgcatc acgatgcggg
     5821 tgaacaggcg gtcgtacatc ggatcgcgtg ggtatttgcc agtcagcggg tcgcaattgg
     5881 ttgcaaacgc ggtgtccagc tccttccagt cctcgtcgga cagcaccttc cttgctatgg
     5941 gaagaatggc agattcttcg aggtgcatgt gctccaggta ggcagcgata tagcgccggg
     6001 tcgcttcttc aaatgccacg cggcgggaat cacctaccag ttcccacgcc agcagcaggt
     6061 gctgcaattc acgtactccc gcttcgctca tggcatggtc tttttccagg cgggcaatgg
     6121 cttcctgggt ctctggcgcc agacgtgcaa cccgcggaaa caacaagttg gattccttgg
     6181 tgtggtgcaa cttctcggga aactcatcca catagaaaag catggctcgc aacacatcga
     6241 agtagcgctc cggttcatcg tccggccccc gatccaccat catgctgagt gagcgcagca
     6301 tggcggccag tgccgtatgt tcttcctgaa tgatctgcag gctttgtctg agcatgtcgt
     6361 gtctccttat tggagtacaa ggttcgcaca gccgcagcgg gcggatattg attcaggtca
     6421 accctgttta agctgagcca atccgcagcg actgacccgc atgcaccgca ccctcgcgca
     6481 ggcggaactg ccgcaccagc tgggccaggc gctctgcctg ctcgcgcagg gactctgctg
     6541 ctgccgccga ctgctccacc agtgcagcgt tctgctgggt catctggtcg atttcggtca
     6601 ccgtctggtt cacactggaa ataccctgcg actgctccac cgatgccgag gaaatttccc
     6661 caatgatgcc gcccacccgc tgcgcgccac tgaccatttc ctgcatggcc gtgccggctt
     6721 cttcggccag gcgcacgccg ccatcgaccg cggtgacact ggcctgaatc aggtgcttga
     6781 cgtcgttggc agcctgggcc gaccgctgcg ccagcgaacg cacctcgctg gccaccaccg
     6841 caaacccgcg cccctgctca ccggcacgcg ccgcctccac ggcggcgttg agtgccagga
     6901 tgttggtctg gaacgcaatc gaatcgatca acccgatgat gtcgccaatc ttgcggctgg
     6961 cacccgaaat ctcatgcatg ctggtcacca catgcgtaac gacctcgcca ccgcgtgaag
     7021 cctggccgga ggccgcgctg gccagctggt tggccgtctg cgcggacgat gcagtctgct
     7081 gcacagtccc caacagctcg gaaatagacg acaccatgct ctgcacattg ctggccgcgc
     7141 gttcggtccg caccgacaag tcctggttgc cggaggcaat ttcctgactg gcggaggcaa
     7201 tgttttcact ggcgtcgcgc atgtggccca cggtattggc cagactgtca cgcatgccgt
     7261 caagcgaccg catcaggtcg gacagctcgt cactgccctc cacacggata tccaccgaca
     7321 ggtcaccact ggtaatcgcc tgggcaaaac ctcgcgcctg ttccaccggc ttgcagatgg
     7381 acagcatgtt caaccaggtc aacggaccca ccaccacaat ggtgagcacc aaggccaggg
     7441 caaacagcag cttggaagtc ccgctggcgt cctcctgttc cttcacggcg gcaccggcct
     7501 gcttctgcac cagatcctcc agcgccagaa gctgcttttc cagctcggca aactcctggt
     7561 tggccttggt cagcaaacta ttgcccacgg tggcactggg cactgcccca ccggagagct
     7621 gcgtcaccac aggcgccatg gctgcggcat agccatcaat atgggtacgg gcggtttgcg
     7681 cagcacgggc caccgtctgg ctccccgcgc tggagagcgc ctgcgcctgc tccagtgcgt
     7741 tcttgacact gatctgccag cggccatggg catccttcac cgccgtggcc gactcatagt
     7801 ggatgaccat ttccttctcg gccacacgcg tacggcccaa ttcaaactgg agccgggaga
     7861 gacgcccggc atcggcaaac gtatggtcca tgaagtcctg gctgagcgcc tgcatgcgga
     7921 acatgctcca cagtcccacg ccccccagaa tcgagagcag gcccagcacc acagcaatgg
     7981 cactgagcat gcgaaagcga atcgtaaaaa ggcgcatcag ggtcagcatg gggatatctc
     8041 caacagttct taacgttcag ggtttccagc gcttgagcaa caacgcgttg ctcatcacac
     8101 tcaccgaact catggccatg gccgcacctg ccagaaccgg gttcagatac cccaatgccg
     8161 ccagcggtat gcccgcagca ttgtaggcaa aagcccagaa cagattctgt cgaatctttg
     8221 acactgtgcg gtctgaaata tcgagtgccg ccgccaccag cagggggtcg ccgcgcatca
     8281 gggtgatacc cgccgcgtgc atggccacgt ccgttcctcc gccaccgggg ttggccatcg
     8341 ccatgcccac atcggctgcc gtcagggccg gtgcatcatt gacgccatca cccaccatgg
     8401 ccacgacatg accaccctgc ttcaaggcca ccacgtgcgc agccttgtca ccgggcagga
     8461 cttcagccat cacctcgccg gcctgtgggt ccagcccaag gcgtcgcgcc atggcctcgg
     8521 cagcaccccg gttgtccccg gaaatcatca cggtcttgat gccgcgggcg cgcaaagccg
     8581 ccagcgccgc tttcgcaccc ggcttgggtt catcgccgaa agccagcagg gccaagggcc
     8641 gcagaccgcc ggtgctgcgc tccacgaagg ctgaaaccgt ggcgccctct gattggaggg
     8701 cactggcctg ttcagccaag gcctccaggt gcacaccgag ttcctccatc cagcgcagac
     8761 tgcccagcag aaagctgcgc acccccactt cgccttccgt gccccgcccc ggcacagcgc
     8821 gcacattgtc gggtgcctcg aacgccacac cccgctcccg cgccgcatcc atcacggcgc
     8881 gggccagcgg atgctcgctg ccactttgca gactggccac cagggccagc acttgcgcct
     8941 cgtcggaccc ctgcgtcggc accagacggg tcagcaccgg gcgccccacg gtcagcgtac
     9001 cggtcttgtc gaatgccacc acgtccacct tgtgggccag ctccagggcc tgcgcgtcct
     9061 tgatcaggat gccgtatttc gccgccacgc cggtccccgc catgatggcc gcgggcgtag
     9121 ccaggcccag ggcacagggg caggcaatca ccagcaccgc cacacagtaa atcagcgact
     9181 gctcaaacga atgcccggtc agccaccagg ccagcagggt caccagggca atcaccagca
     9241 ccaccggcac gaacaccgca ctgacctggt ccacaaggcg ctggatgggg gccttggccg
     9301 cctgggcatc ctcgaccagc cggatgatgt gcgaaagcac cgtctcactg cctacggcac
     9361 gcacctgcat gaccacccgg ccatcgccgt tgatggagcc ccccgtgagc ttgccaccgg
     9421 gctcc
//
LOCUS       DAOSJM010000044         9365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7028_length_9365_cov_4.538346, whole genome shotgun sequence.
ACCESSION   DAOSJM010000044 DAOSJM010000000
VERSION     DAOSJM010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9365
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7028_length_9365_cov_4.538346"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1924)
                     /locus_tag="PKX82_02675"
     CDS             complement(<1..1924)
                     /locus_tag="PKX82_02675"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOF51181.1"
                     /translation="MGSALPIKPVDSAANAAAASFAGLTAKLDYLSAQDIEQVRLAYR
                     FADEAHLGQLRNNGEPYITHPIAVAAQCAEWKLDAQALMAALLHDALEDCGVTKIELI
                     ERFGSPVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDVRVILIKLADRSHNM
                     RTLSDVPRSKWNRIASETLEIYAPIAHRLGLNQTYRELQDLSFQHLKPWRYNILKKAV
                     TKARSRRRDLIQKVQKEVEAVFSEADIPVRIAGREKTLYSIYQKMTNKHLSFAQVTDI
                     YGFRVLVPTVIDCYTALGLLHQLYKPVPGKFKDHIAIAKLNGYQSLHTTLVGPSGINI
                     EFQMRTEAMHVVAESGVAAHWLYKVNHPDDTSSERLGTKWLQSLLDIQDETRDAAEFW
                     DHVKVDLFPDAVYVFTPKSQIMALPRGATVVDFAYAIHSNVGDRTMAARINGEQVPLR
                     TELKNGDVVEVITAPVSTPNPAWLSFVRTGRARSKIRHHLKTMAQTESENLGERLLHQ
                     ALRSEGIEHFPDGDPQYAPLWDKLLRFTGNKTRHELLTDIGLGKRIANIVAKRLLHLL
                     VESGERPDALLLTRERFTAHESPSMGLITLDGSENASVQFAQCCRPIPGDAIVGYLGR
                     GEGLVVHASDCQIAKKL"
     gene            complement(2015..2218)
                     /gene="rpoZ"
                     /locus_tag="PKX82_02680"
     CDS             complement(2015..2218)
                     /gene="rpoZ"
                     /locus_tag="PKX82_02680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOF51182.1"
                     /translation="MARITVEDCLEKIPNRFQLVLAATYRARMLSQGHTPKIESKNKP
                     GVTALREIAAGKVGIEMLKKVPL"
     gene            complement(2236..2856)
                     /gene="gmk"
                     /locus_tag="PKX82_02685"
     CDS             complement(2236..2856)
                     /gene="gmk"
                     /locus_tag="PKX82_02685"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOF51183.1"
                     /translation="MDYPGNLFVVAAPSGAGKSSLVKALLELDSRVQPSVSHTTRPPR
                     GQEKHGREYFFVSQQEFDAMVAADAFVEWAHVHSQRYGTSKKAIEERMALGADVILEI
                     DFQGAIQIKNIFSNAICIFILPPSWEELRSRLERRGEDAPDIIDLRMKNAQAEVAHVD
                     KFDFVIINELFDRALFDLKAIVHSQRLKFSAQRRARAETFKALNIP"
     gene            complement(2868..3785)
                     /locus_tag="PKX82_02690"
     CDS             complement(2868..3785)
                     /locus_tag="PKX82_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOF51184.1"
                     /translation="MPVYSMTGYASAQHSTANTPPENEGKNIPATQLGLEIRSVNSRF
                     LDLSFRLPEDLRQFEPALRDLIVGKLKRGKVEVRASIETAGATGVPEPTPKLLQRLNA
                     AQDTVKSWLPQAGALSVADVLRIAATEQSGTRDWSASVMPLAEKSLKELLSARQREGA
                     RLSAMLTERLEQLRALAQQAVPLVPQLVAQQRQRFLDRWKEAMSLADGSTLPEAAQDR
                     ALTEATAFAIRIDVAEEVTRLNSHLDEIERLLKKGGEVGKRLDFLIQELHREANTMGS
                     KSSALELTHISVDMKVLIEQMREQVQNIE"
     gene            3865..4869
                     /locus_tag="PKX82_02695"
     CDS             3865..4869
                     /locus_tag="PKX82_02695"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOF51185.1"
                     /translation="MSKVKPAPLPPDTMIGGYRIIRKVAAGGFGLVYLAVDADGQQVA
                     VKEYLPSSLASRAPGELLPQVQPEKLSLYRLGLKSFFEEGRSLAQISHPSVVSVLNFF
                     RENETVYMVMNYLEGAALQDFIVTARDLKQSKVFRESTIRSLFDEILRGLRIVHQHKM
                     LHLDIKPANIFITDDNKSVLIDFGAAREVLSKEGNFIRPMYTPGFAAPEMYRRDASMG
                     PWTDIYAIGACIYACMQGYPPNEAPQRLEKDRIAIALARLRGVYSDNLIEVVEWCMSL
                     DPLSRPQSVFALQKELSREGERKYTKLSVTEKVRMQFDTMVADTKKTVQGAAPGTKTK
                     "
     gene            4866..5780
                     /locus_tag="PKX82_02700"
     CDS             4866..5780
                     /locus_tag="PKX82_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HOF51186.1"
                     /translation="MKFSVFQISRKGGREKNEDRMGYCYTRTSGLFLLADGMGGHPQG
                     EVAAQIALQVISAMYQKQAQPEIVDVPAFFTGAVMAAHHQILRYAVEKGLLDTPRTTL
                     VAAIVQNGVAHWVHCGDSRLYFVRQGELLARTRDHSYLAQRHDSSRVGGPQADISRNV
                     LYTCLGSPTKPVYDQTGPVALQQGDRMMLCSDGLWGTLEDIDIVFYLSQKSIVVAAPE
                     LVERALLKGGPHCDNVTLVAMEWETPDAFVSTRGSISTDDIEDGVFASTVQAGGLDVG
                     EDDLDDAAIERSIAEINEAIRRSAARRS"
     gene            5787..6515
                     /gene="rph"
                     /locus_tag="PKX82_02705"
     CDS             5787..6515
                     /gene="rph"
                     /locus_tag="PKX82_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HOF51187.1"
                     /translation="MQVFVRSSNRAANQLRPVRLTRGYTVHAEGSVLIEFGGTKVLCT
                     ASVQEKVPPHKKGSGEGWVTAEYGMLPRATHTRSDREAAKGKQSGRTQEIQRLIGRSM
                     RAVFDLKKLGERTIALDCDVLQADGGTRTAAITGAFVAAQDAVNGLLAAGKITESPIT
                     AQVAAISVGIVQGTPLLDLEYTEDSACDTDMNVVMTGAGHFVEVQGTAEGAAFTRAEM
                     DALLGLAESGISELMELQRQSLSK"
     gene            6559..7158
                     /gene="rdgB"
                     /locus_tag="PKX82_02710"
     CDS             6559..7158
                     /gene="rdgB"
                     /locus_tag="PKX82_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOF51188.1"
                     /translation="MKIVLASNNKGKLAELQALFSPLGCTLIAQSDLGVPEAPEPHRT
                     FVENALAKARNASLHTGLPALADDAGLCVDAFGGLPGVDTAYYATQFGYPKGDDNNVR
                     ALLEQMQNIDNRRAALVSTLVAVRSPQDPEPLIAMGRAQGLITREPVGSNGFGFDPVM
                     WLPEFGQTFSQLPVEVKNANSHRGRAAQAMVAQMRERWL"
     gene            7212..8384
                     /gene="hemW"
                     /locus_tag="PKX82_02715"
     CDS             7212..8384
                     /gene="hemW"
                     /locus_tag="PKX82_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOF51189.1"
                     /translation="MRPGTLQLAALPPLSLYVHLPWCIRKCPYCDFNSHEMRRDVLPE
                     DDYVRAVVADLESALPLIWGRSVHSIFIGGGTPSLFSPAAIDRLLGDIRARLRLEPDC
                     EISMEANPGTFEKDRFRAYRSAGVTRLSIGVQSFNDAHLQALGRVHDRAQALAAVEEA
                     ASAFDTFNLDMMYALPGQSMAQVEDDMRTALQFAPPHISIYHLTIEPNTYFAKYPPSI
                     PEDDTAYAMLDHITELTAAAGLDRYEVSGYARAGHRCRHNLNYWQFGDYLGLGAGAHS
                     KLSFAHRVVRQVRLRDPQRYLERALQGNAVAQDEEVSRADLPFEFMLNALRLRHGFGL
                     REFTERTGLPVTAIQRALEEAETKGLIERDFARVRPTQRGFDFLSDLQALFLSNAD"
     gene            8417..9085
                     /locus_tag="PKX82_02720"
     CDS             8417..9085
                     /locus_tag="PKX82_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOF51190.1"
                     /translation="MFSVYGRAGRLFRGSMEDLHKVGAMSALARSRRVAPIGQDAQDQ
                     TTPLPEAHHAAPMDVAHRSAMAAYAQTTKPEIHRHPLTHVEDIVTRSVVTIADTATIG
                     DAWAMLAERGIGQVPVVGADGVLVGLLTRADLTRPDRLPGPEMHALVWRALLAQRVVD
                     MMWSPVPSVAMDTDIRRVARVLLDTGLPGLPVVDDAGRVTGFVSRTDILRAVVADPPL
                     DLWT"
BASE COUNT         1752 a         2731 c         2984 g         1898 t
ORIGIN      
        1 gcagcttttt tgcgatctgg caatcgctgg catgcactac cagaccttct ccgcgcccaa
       61 ggtagcccac aatcgcatcc ccgggaatgg ggcgacagca ctgcgcaaac tgcacggacg
      121 cgttctcgct tccgtccagc gtaatcaggc ccatggaagg cgattcatgg gctgtgaacc
      181 gctcccgcgt cagcaggagg gcatcgggtc gctcgccgga ctcgaccaga aggtgcagca
      241 gccgtttggc gacgatgttg gcgatccgct tgcccaggcc aatatctgtg agcaactcgt
      301 gtcgggtctt gttgccggta aaccgcaaca gcttgtccca cagcggggcg tactgcggat
      361 ctccgtcggg gaagtgctca atgccctccg aacgcagcgc ctggtggagc aggcgctcgc
      421 ccaggttttc ggattcggtc tgcgccatcg ttttgagatg gtggcgaatt ttggatcggg
      481 cacgtccggt gcgcacgaaa ctcagccagg ccgggttggg ggtggacacc ggagccgtga
      541 tcacttccac cacatcgcca ttcttcagct cggtgcgcaa gggcacctgt tcgccgttga
      601 tgcgtgcggc catggttcgg tcgcccacgt tgctgtggat ggcatacgca aaatccacca
      661 cggtggcgcc gcgcggcaac gccatgatct ggctcttggg cgtgaacacg tagaccgcat
      721 ccgggaacag atcgaccttg acgtggtccc aaaactcggc agcgtcccgc gtttcgtcct
      781 ggatgtccag cagggactgc aaccatttgg tgcccagacg ttcactggag gtgtcgtccg
      841 gatggttgac cttgtagagc cagtgtgctg cgacgcccga ctcggcgacc acatgcatgg
      901 cctcggtccg catctggaac tcaatattga ttcccgaggg gcctaccagt gtggtgtgca
      961 gggactggta gccattgagt ttggcaattg caatatggtc cttgaacttg ccgggaaccg
     1021 gcttgtacaa ctggtgcagc agacccagcg cggtgtagca gtcaatgacc gtgggcacca
     1081 gcaccctgaa cccatagatg tcggtcacct gggcaaagct caggtgcttg ttggtcattt
     1141 tctggtaaat cgaatagagc gttttttccc gtccggcgat gcgcacggga atgtcggcct
     1201 cggaaaaaac cgcctcgact tccttctgca ctttttgaat caggtcgcgc ctgcggctac
     1261 gagccttggt cacggccttt ttcagaatgt tgtagcgcca gggcttgagg tgctggaaag
     1321 acaggtcctg caactcacgg taggtctggt tcaaacccag gcggtgcgca ataggcgcgt
     1381 agatttcaag agtctcggac gcgatgcggt tccatttgga acggggtacg tcggacagcg
     1441 tgcgcatatt gtgcgaacgg tcggccagtt tgatcaggat gacgcgaaca tcgcgcgcca
     1501 tggccagcag catcttgcgg aaggactcgg cctggttctc ttcgcgggtg ttgaactgca
     1561 gcttgtcgag cttggtcagg ccatcgacca gttccgctac cggagacccg aagcgttcaa
     1621 tcagttcgat tttggtaacg ccgcagtctt ccagcgcatc atgcaggagc gctgccatga
     1681 gggcttgcgc atcaagcttc cactcggcgc actgtgccgc gaccgcaatg gggtgcgtga
     1741 tgtagggctc accgttgttg cgcaactggc ccaggtgcgc ttcatccgca aaacggtagg
     1801 ccaggcgaac ctgctcgata tcctgcgccg agagatagtc cagcttcgcc gtcaggccgg
     1861 caaagctggc cgccgctgcg ttcgcggcgg aatctaccgg cttgattgga agtgcgctac
     1921 ccatgccgta aatgtagcgc cagcaaggca aaccagggtt gcatcagggc aaaaaaaagc
     1981 accgcgtacg gtgcttttcg ggagtgaaag tggctcagag aggcactttt ttcagcattt
     2041 caatgccgac tttgcccgca gcaatttcgc gcagggcagt gacgccgggc ttgttcttgc
     2101 tttcgatctt gggcgtgtgt ccctggctca gcatgcgagc gcggtacgta gccgccagta
     2161 ccaactggaa gcggttgggg attttttcga ggcagtcttc gacagtgata cgggccatgg
     2221 tgtttctcca aaaggctagg ggatgttcaa cgctttgaat gtttcggccc gggcgcgtcg
     2281 ctgcgccgag aatttgagcc gctgggaatg aactatggcc ttcagatcga aaagcgcacg
     2341 gtcaaacaac tcgttgatta taacgaagtc gaatttatcc acatgggcga cctccgcctg
     2401 ggcgttcttc atgcgcaggt caatgatgtc gggcgcgtct tcaccgcggc gctccaggcg
     2461 ggagcgcaac tcttcccagc tgggtgggag gatgaagatg cagatggcat tggaaaagat
     2521 attcttgatc tgaatagctc cctggaagtc gatttccagg atcacatcgg cgcccagcgc
     2581 catgcgctct tcaatggctt tcttggaggt accgtagcgc tggctgtgca catgcgccca
     2641 ttccacgaag gcgtcggccg ccaccatggc gtcaaactcc tgctgcgaga caaagaagta
     2701 ctcgcggccg tgtttttcct gcccccgggg cgggcgggtg gtgtgggaga cagagggctg
     2761 gaccctggag tccagttcca gcagggcctt gaccaggctg gatttgcccg ccccgctggg
     2821 ggcggcgaca acaaagaggt ttccaggata gtccatggca aaagccctta ttctatgttc
     2881 tgtacctgtt cgcgcatctg ctcgatgagc accttcatat cgaccgagat gtgggtcagt
     2941 tcgagtgccg aggacttcga tcccatggtg ttggcttccc gatgcagctc ctggatcagg
     3001 aagtccagcc gttttccaac ttctccgcct tttttgagca ggcgttcaat ttcatccagg
     3061 tgggagttca ggcgggtgac ttcctcggcc acatcgatgc ggatggcaaa cgcggtcgct
     3121 tcggtcaggg cgcggtcctg ggctgcttct gggagcgtgg agccgtctgc cagtgacatg
     3181 gcttctttcc agcggtccag aaagcgctgg cgctgctgtg ccaccagttg cggcaccagt
     3241 ggcacggctt gttgggccag ggcgcgcagc tgttccagcc gttcggtcag catggcggag
     3301 agacgggcgc cttcgcgctg gcgtgcgctc aggagttcct tgagggattt ttcggccagc
     3361 ggcatgacgc tggcactcca gtcgcgcgta cccgattgtt ctgtggctgc gatgcgcagt
     3421 acatcggcaa cgctcagcgc accagcctgc ggcagccagg atttcacggt gtcctgggcg
     3481 gcgttgaggc gctggagcag tttgggtgtg ggctcgggaa cacccgtggc gccagcggtt
     3541 tcaatggaag ccctgacttc cactttgccg cgtttgagct tgccgacgat cagatcgcgc
     3601 agcgcgggct caaactgacg caggtcctcc ggcaaacgga atgaaaggtc cagaaagcgg
     3661 ctgttaaccg accggatctc cagccccaac tgggttgcgg gaatgttttt cccctcgttt
     3721 tcgggagggg tattggctgt gctgtgctgt gcactggcat agccagtcat gctgtaaact
     3781 ggcatcgcaa gttctcgggt cgaaagaatg gcccagagaa taacccggtt ccgccgcttc
     3841 agacgagtcc tttagttcga atctatgtca aaggtaaaac cagctcccct tccccccgat
     3901 acgatgatcg gaggctaccg catcatccgc aaagtggcgg ctggcggttt cggactggtg
     3961 tatctggcag tggatgcgga tggccagcag gtcgccgtga aagagtattt gccctcgtcg
     4021 ctggcgtcac gggcgccagg agagttgctg ccgcaggttc agccggaaaa gctctcgctg
     4081 tatcgcctgg gcctcaagag cttttttgaa gaaggccgtt cgctggcgca gatttcgcac
     4141 ccttccgtgg tgagcgtgct gaatttcttc cgcgaaaatg aaaccgtcta catggtgatg
     4201 aactacctgg agggcgcggc cctgcaggat ttcatcgtga ccgcgcgtga cctcaagcag
     4261 agcaaggtat ttcgcgagtc caccatccgg tcgctgttcg atgaaatttt gcgtggactg
     4321 cgcatcgtgc accagcacaa gatgttgcac ctggacatca agccggccaa catctttatt
     4381 accgacgaca acaagtcggt gctgatcgac tttggtgcgg cccgcgaggt gctgagtaaa
     4441 gagggcaatt tcatccgccc catgtacacc cccggctttg ccgcgccgga gatgtaccgt
     4501 cgcgatgcgt ccatgggtcc ttggaccgac atttacgcca ttggtgcctg tatctatgct
     4561 tgcatgcagg gatatccacc caatgaagcg ccccagcgac tggaaaaaga ccgcattgcc
     4621 attgccctgg cgcgcctgcg tggtgtgtat tccgataacc tgattgaggt ggtggaatgg
     4681 tgcatgtcac tggacccgct gtcccgtccg caatcggtat tcgcattgca gaaggaattg
     4741 agccgcgaag gcgaacgcaa gtacaccaaa ctcagcgtga ccgagaaggt gcgcatgcag
     4801 tttgacacca tggtcgccga caccaagaaa accgttcagg gtgcagcacc cggaaccaag
     4861 accaaatgaa attctccgtt tttcagatca gccggaaagg cggtcgcgag aaaaacgaag
     4921 accgcatggg gtactgctat acccgcacct cgggcctgtt cctgctggcc gacggcatgg
     4981 gcgggcaccc ccaaggcgag gtggcggcgc agattgcact gcaggtgatc tcggcgatgt
     5041 accagaagca ggcgcagccg gaaattgtgg atgtgccggc cttcttcacc ggagcggtga
     5101 tggccgccca ccaccagatc ctgcgctatg cggtggaaaa gggactgctg gacaccccgc
     5161 gtaccacgct ggtggccgcc attgtccaga acggtgtagc ccactgggtg cattgcggtg
     5221 attcgcgtct gtatttcgtg cgccagggcg agttgctggc gcgcacgcgc gaccactcct
     5281 atctggcaca gcgccacgac agttccaggg tgggcgggcc gcaggcggat atcagccgca
     5341 acgtgctgta cacctgcctg ggttctccga ccaagccggt atacgaccag accgggccgg
     5401 tagcgctgca gcagggtgat cgcatgatgc tgtgctccga cggcctgtgg ggcacgctgg
     5461 aagacatcga catcgtgttc tatctgagcc agaagtccat tgtggtggca gccccggagc
     5521 tggtggagcg ggcgttgctc aaaggtggtc cccactgcga caacgtcacc ctggtggcga
     5581 tggagtggga gactccggac gcgtttgtgt cgacccgcgg cagcatctcc accgatgaca
     5641 ttgaagacgg tgttttcgca tccacggtgc aggctggcgg cctcgatgtg ggcgaagacg
     5701 accttgacga tgccgcgatt gagcgctcca ttgcagaaat caacgaggcc atccggcgct
     5761 cggcagcccg cagatcctga aaatccatgc aagtttttgt ccgtagcagc aaccgcgctg
     5821 caaaccagtt gcgcccggtg cgtttgaccc gcggctacac cgtccacgcc gaaggctcgg
     5881 tgctgatcga atttggtggt accaaagtgc tgtgcacggc ctcggtgcag gagaaagtgc
     5941 caccgcacaa aaagggcagt ggtgaggggt gggtgacggc ggagtacggc atgctgccgc
     6001 gcgcgaccca tacccgcagt gaccgcgagg cggccaaagg caagcaaagc gggcgcaccc
     6061 aggaaatcca gcgcctgatt ggccgttcca tgcgcgcagt gtttgacctc aagaagctgg
     6121 gcgagcgcac cattgccctg gattgcgatg tgctgcaggc cgatggaggc acccgcaccg
     6181 cggcgatcac cggtgccttt gtggcggcgc aggatgccgt caacggcctg ctggccgcag
     6241 gcaagatcac cgagtccccg atcaccgcgc aggtggctgc catctccgtg ggcatcgtgc
     6301 agggcacgcc tctgctggac ctggaataca ccgaagactc cgcctgtgac accgacatga
     6361 atgtggtgat gactggcgcc gggcactttg tcgaagtgca gggcacggcc gaaggcgcgg
     6421 cattcacccg cgccgaaatg gacgccctgc tggggttggc cgaaagcggg atttccgagc
     6481 tgatggaact gcagcgtcaa tcgctatcaa aatgatagct gtctgcgcaa tctgtgtgcg
     6541 ggctagaggc caatttctat gaaaatcgtc ctggcgtcca ataacaaggg caagctggcg
     6601 gagctgcagg cgctgttcag cccgctgggc tgtacgctca tcgcgcaaag cgacctcggc
     6661 gtgcccgagg ccccggagcc gcaccgcacc tttgtcgaaa acgcactggc caaggcgcgc
     6721 aatgcgtcgc tgcataccgg gctgcccgca ctggccgacg atgcgggcct gtgtgtggat
     6781 gcgtttggcg gcttgccggg tgtggacacc gcgtactacg cgacgcagtt tggctatccc
     6841 aaaggagatg acaacaacgt gcgggcgttg ctcgaacaga tgcagaacat cgacaaccgc
     6901 cgcgctgcgc tggtcagcac gctggtggcg gtgcgttcgc cgcaggaccc ggagccactg
     6961 attgccatgg gccgcgcgca gggactcatc acgcgcgagc cggtcggcag caatggcttt
     7021 ggttttgatc cagtcatgtg gctccccgag tttggccaga ccttttcgca gctgccggtg
     7081 gaagtcaaaa atgccaacag ccaccgcggc cgcgccgccc aagccatggt ggcccagatg
     7141 cgggagcgct ggctgtgata ccggtggtgc ccgctgttga gacggcggtg gtgcacgacg
     7201 tgcagcatta catgcggccc ggcacgctgc agctggcggc gttgccaccg ctgtcgctgt
     7261 acgtgcactt gccgtggtgc atccgcaagt gcccctactg cgatttcaac tcccatgaaa
     7321 tgcgccgcga cgtgctgccg gaggacgact atgtccgggc cgtggtagcc gacctcgaat
     7381 ccgcgctgcc gctgatctgg gggcgcagcg tgcacagcat tttcatcggc ggcggaacgc
     7441 ccagcctgtt ctcgcctgct gccattgacc gcttgctggg cgatatccgg gcccggctgc
     7501 ggctggagcc cgattgcgaa atttccatgg aagccaatcc cggcaccttt gagaaagacc
     7561 ggtttcgggc ctaccgcagc gcgggcgtga cccggttgtc gattggggtg cagagcttca
     7621 acgatgcgca cctgcaggcc ctgggccgcg tgcacgaccg ggcgcaggca ttggcggcgg
     7681 tggaagaagc ggccagcgct ttcgatacct tcaacctgga catgatgtat gccctgcccg
     7741 ggcaaagcat ggcgcaggtg gaggatgata tgcgcaccgc gctgcagttt gcgccgcccc
     7801 atatttcgat ctaccacctg acgatcgagc ccaacaccta tttcgccaag tacccgccca
     7861 gcattccgga agacgatacg gcctatgcca tgctggacca cattaccgag ctcacagcgg
     7921 ctgcgggtct ggaccgatac gaagtgtcgg gctatgcacg cgcagggcac cggtgtcgcc
     7981 acaacctcaa ttactggcag tttggagact atctggggct gggtgcgggt gcccacagca
     8041 agctcagctt tgcgcatcgg gtggttcggc aggtgcgcct gcgtgaccct cagcgctatc
     8101 tggagcgcgc actccagggc aatgcggtgg cacaggatga agaagtcagc cgcgcggact
     8161 tgccttttga gttcatgctc aatgcattgc ggctgcgcca tggttttggg ctgcgcgagt
     8221 tcaccgagcg cacggggcta ccggtcaccg ccatccagcg cgccctcgaa gaagccgaga
     8281 ccaaggggct gatcgagcgg gactttgccc gtgtgcggcc cacgcaacgc ggctttgact
     8341 ttctcagtga cttgcaggcg cttttcctgt ccaatgcgga ctagcgtgtg ctgggttgca
     8401 gtcaggagcg aatgaaatgt tctcggtcta tggcagggca ggacgtctgt ttcggggatc
     8461 catggaggac ttgcacaaag tcggtgccat gtcggcattg gcgcggtccc gtcgtgtcgc
     8521 ccccataggg caggacgcgc aggaccagac cacacccctt cccgaagcac accacgctgc
     8581 acccatggac gtagcccatc gcagtgccat ggctgcctac gcccagacga ccaaaccaga
     8641 gatccatcgc cacccgttga cccatgtgga ggacatcgtg acgcgttcgg tggtgaccat
     8701 tgcggacact gccacgatcg gcgatgcgtg ggcgatgctg gctgagcgtg gcatcggcca
     8761 ggtgcccgtg gtgggggccg acggtgtgct ggtggggctg ctgacgcggg ccgacctgac
     8821 gcgcccggat cgcttgccag ggccggaaat gcatgccctg gtctggcgtg cgctgctcgc
     8881 gcaaagagtg gtggacatga tgtggtcgcc cgtgcccagc gtggccatgg acaccgatat
     8941 ccgccgtgtg gcgcgggtgc ttctggatac cggcctgccg gggttgcctg tggtggatga
     9001 tgcgggccgg gtgaccggtt ttgtctcccg caccgatatc ctgcgtgcgg tggtggccga
     9061 tcccccgctg gatctctgga cttgacgtgc tgcacacggg caggtagccg gggccatcgt
     9121 tgccagcgtg ctggatgatg gcgatggact tcactggcaa tgcggggagg gaatgggagt
     9181 ggcggaagct gtgtcattag aaccactagc atccatgcgg ttttccaatg gattttccat
     9241 cagtacccac ataggtgccc acaattgtga aagtgtgcaa cagccgtttg gtgctttgat
     9301 tgtagctttc gacaacccag ttctgggtat ctacagccca cagtcgccat gccttcagca
     9361 ctgtg
//
LOCUS       DAOSJM010000045         9082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7252_length_9082_cov_4.474465, whole genome shotgun sequence.
ACCESSION   DAOSJM010000045 DAOSJM010000000
VERSION     DAOSJM010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9082
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7252_length_9082_cov_4.474465"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PKX82_02725"
     CDS             <1..357
                     /locus_tag="PKX82_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOF51191.1"
                     /translation="QVGDASEADWSEEYLAPIVSVKVVEGVDEAIAHINQYSSHHTDA
                     ILTRDHMHAQRFLREVDSASVMVNTSTRFADGFEYGLGAEIGISTDKFHARGPVGIEG
                     LTSLKYVVLGEGEVRS"
     gene            435..1715
                     /gene="gshA"
                     /locus_tag="PKX82_02730"
     CDS             435..1715
                     /gene="gshA"
                     /locus_tag="PKX82_02730"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOF51192.1"
                     /translation="MVPHLVTALTGPINELEQRVLDSMPAIERWFRLEWMEHTPPFYT
                     SVDVRNAGFKLAPVDTDFFPASWNNLTESMLPLAVQAAMAAIEKICPEARNLLIVPDN
                     NLRNTSYLSNLAQLRRVFHMAGLNVRIGSISPDVKKATTIELPGGEIITLEPVVRTKG
                     RLGVKNFDPCTILLNTDLSAGVPGILEDLNEQYLLPPLHASWTTRRKSTHFKCYEEVS
                     KRFGKLIGVDPWLINPLFDKCGSVDLDTEAGMQTLATHVDALLGKVRKKYKEYGIKEK
                     PFVILKTDDSSDGLGITLRDVKDLDAVRAKGSAFGSLQGVRELILQEGVLTRERMNDA
                     VAEPVVYMMDRYVVGGFYRIHAERGVDENLNAPGSSYVPLAFAESTHLPQPGERPGVS
                     APNRFYMYGVVARLAMLAASYELEATDPDAEHYD"
     gene            1877..3745
                     /locus_tag="PKX82_02735"
     CDS             1877..3745
                     /locus_tag="PKX82_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HOF51193.1"
                     /translation="MSASKSSLAALTLGAIGVVYGDIGTSVLYAMKEVFGSGHVPFTR
                     DNVFGILSIFFWTLTVIVSIKYVVLVLRADNHGEGGLVAMLALASQSVKDRPELRRVL
                     LLVGIFGTCLFYGDGVITPAISVLGAVEGLEVISPTFKKFVIPLTLVILLALFMVQKH
                     GTASMGRFFGPIMVVWFAVIAGLGLVNIAADPLILAAISPHYAVGFMWDNPGTTFIIL
                     GAVVLCVTGGEALYADMGHFGKKPIRVAWFMVAMPALTLNYFGQGALLLHNPAAVKNP
                     FFMMAPDWALIPLVGLAAMAAVIASQALISGAFSVTKQVIQLGYLPRLQIQHTSVKDT
                     GQIYLPFVNWGLFALIVVAVVMFKSSSNLAAAYGIAVCTDMLITTVLTFYVIRYSWKY
                     PLALCIGATGFFFIVDFAFWASNLLKLFEGGWFPLMIGGAIFTLMLTWKDGRRLLNEK
                     LRADSLDLPSFLEAVFVSPPARVEGTAVFLTADVGTVPNAMLHNLKHNKVLHVNNLFV
                     TVRNHEVPWIGLDKRIEIDSLGHDCWQVVIHYGFKNDPDVPKALQQIKGRGCELDPMS
                     TSYFLSRDTVVPTLGEGMAQWREKLFAQMHHNASGAADFLNLPNNSVVELGSKIEI"
     gene            3760..4713
                     /gene="gshB"
                     /locus_tag="PKX82_02740"
     CDS             3760..4713
                     /gene="gshB"
                     /locus_tag="PKX82_02740"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HOF51194.1"
                     /translation="MDILFVADPLESFKIYKDTTFSMMREAQRRGHRIIACEPRHLIW
                     QKGGVVRAHVRHITLTGHEERWFHETPMPPESRLQALYDFDAIVMRKDPPFDSEFFYA
                     THLLEQAEREGAKVFNKPRALRDHPEKLAILEFPQFIGPTLVTRDADAVRAFHAEHRD
                     IILKPLDGMGGMGIFRVKDDGLNLGGIIETLNKDGAQTLMVQKFLPAIAQGDKRVLVI
                     GGKPVPFCLARIPQGGEVRGNLAAGGKGVAQPLTDADRAIAEALGPVLAARGLLLVGL
                     DIIGDSLTEINVTSPTCFQEITDQTGFDVPAMFVDALEAAL"
     gene            4845..5429
                     /locus_tag="PKX82_02745"
     CDS             4845..5429
                     /locus_tag="PKX82_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOF51195.1"
                     /translation="MKKALLLAASVAFIAPQAFAQAKNFEGFSVGANLAVAKSTTEPT
                     GVASESGNTNSLDLQAQYSLALAPQFVLGLGVTLGTGNNNAGTFSLTGTEVSTKNRTA
                     FDIIPGYAISNSMMLFGKVSALSADGASTTAGVETKKSISGVGYGFGLRGLIDKNMFY
                     QLGYDINKYNDVDFGTGNVKASSSVFSLGVGYKF"
     gene            5537..6103
                     /locus_tag="PKX82_02750"
     CDS             5537..6103
                     /locus_tag="PKX82_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOF51196.1"
                     /translation="MKFKVLLTALAVASLSTLSAQAQVLGAITASPMTAKTGEPVTIT
                     ANIDVLNANYCGFVVGFGDGAFKDAVSDASNPTPLVLTHTYAKAGNYHVTLGGKNVQS
                     HPNCGGQERAVDIVVTGTTKSAAPAALKAAEVCASGWKLSGKLNAKTGAFTCAAKAGT
                     AFPAAKPECSGDLSYFENTKKGQYGCKP"
     gene            complement(6143..7024)
                     /locus_tag="PKX82_02755"
                     /pseudo
     CDS             complement(6143..7024)
                     /locus_tag="PKX82_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434313.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
     gene            complement(7051..7242)
                     /locus_tag="PKX82_02760"
     CDS             complement(7051..7242)
                     /locus_tag="PKX82_02760"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HOF51197.1"
                     /translation="MPIYHVEMLEGRTLEQKKKLAEEITRVSVEVLGGSPDSVHVVIA
                     EVRRENWAVGGKLWSEPRS"
     gene            7319..8833
                     /locus_tag="PKX82_02765"
     CDS             7319..8833
                     /locus_tag="PKX82_02765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOF51198.1"
                     /translation="MRTDSTFLPRSPALRWLLLALFALHLCLGSLMGLSVDEAHYALY
                     AAHPALSYFDHPPLVGWAQMPLVALQVPDSALRLVPGLLWLATVLGVYRVTVQLHVES
                     QSAGAAAAGLWAVLALALAPLLHILGMGLLPDTLLMALTVALMHQTLTLTELSRPARP
                     IDWLVLGVLLGLAGLSKYTAVLAAMAVAICLLAAHGPRLLRSPWLWLSALVAAILIAP
                     VILWNAQNQWVSFAYQFQHGAGGAWQFPHVLRFLLLQVVVYGPLLLWGGQGLRMVART
                     PSRWLAAFFAIPFALLAYLSGGGTSLPHWTAPAWVALAPFAGMALAHAVQTRARKWVL
                     ALVAVQALLCLALPVLMATGGVPFFQAKAATSAQAPNPFADLHGWDQAGARARSLAAQ
                     HQLDSVSVQNWTLASRLGWYARPMKVHVLEDRFDQFDLWAGDLPQGGNTLLVDWSEMA
                     YVTPVGGNGFASCKLLDVLPVQRLGRPLSEFRFYACNGWSADPKPRLQSEAGAP"
     gene            8830..>9082
                     /locus_tag="PKX82_02770"
     CDS             8830..>9082
                     /locus_tag="PKX82_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51199.1"
                     /translation="MTSAQFNKDVALWVWALPVVLFLVSAPLWLHWWEPQMFVAINTL
                     CMPVAAQFWTGLSLLGNGWGILGVTAPLLVLAPRLMWAWL"
BASE COUNT         1651 a         2621 c         2827 g         1983 t
ORIGIN      
        1 caggtcggcg acgcgagcga agccgactgg tcggaggaat acctcgcgcc catcgtcagc
       61 gtgaaggtgg ttgaaggcgt ggatgaggcg attgcccaca tcaaccagta ttcctcccac
      121 cacaccgatg ccatcctgac ccgcgaccac atgcacgccc agcgctttct gcgcgaggtg
      181 gattcggcca gcgtgatggt caacaccagc acgcgctttg ccgatggctt tgaatacggc
      241 ctgggtgccg agattggcat cagcaccgac aagttccacg cgcgggggcc ggtgggcatt
      301 gaaggcctga cgtcgctgaa gtatgtggtt ctgggcgagg gcgaagtgcg tagctgaggc
      361 ttgcattccg ctttccaccc accatctggt ttcgaccgcc tgcgatgacc gcgggagttt
      421 tttgaaagag cgatatggtt ccccatctgg tgactgccct gacgggcccc attaacgaac
      481 tcgaacagcg ggttctggac tccatgccag ccatcgagcg ctggttccgt ctggagtgga
      541 tggagcacac gccgcctttc tatacctcgg tagatgtgcg caacgccggg ttcaagctgg
      601 caccggtgga taccgatttt ttcccggcta gctggaacaa cctgacggaa tccatgttgc
      661 ccctggcggt gcaggcggcc atggccgcga ttgagaagat ctgcccggaa gcgcgcaatc
      721 tgctgatcgt gccggacaac aacctgcgca acacgtcata cctgagcaat ctggcgcagt
      781 tgcggcgggt gttccacatg gctggcctga atgtgcgcat tggctccatc agcccggatg
      841 tcaagaaagc aacgacgatt gaactgcccg gtggcgagat catcaccctg gagccggtag
      901 tgcgtaccaa agggcgcctg ggtgtcaaga acttcgatcc ctgcacgatt ctcctcaaca
      961 ccgatttgtc cgcaggggtg cccggcattc tggaggatct gaatgagcaa tatttgctgc
     1021 caccgctgca cgcgagctgg acgacccggc gcaagagcac gcatttcaaa tgctacgaag
     1081 aggtcagcaa acgctttggc aagctcatcg gggtcgaccc ctggctgatc aacccgctgt
     1141 tcgacaaatg tggcagcgtg gacctggata ccgaagccgg aatgcagacg ctggcaaccc
     1201 acgtcgatgc actgctgggc aaggtccgca agaaatacaa ggaatacggc atcaaggaga
     1261 agcctttcgt cattctcaag accgacgaca gcagcgacgg cctgggcatt acgcttcgcg
     1321 atgtgaaaga cctggatgcc gtgcgcgcca agggttccgc gtttggcagc ctccaagggg
     1381 tgcgcgagct catcctgcaa gagggcgtac tgacccgtga gcgcatgaac gatgcggtgg
     1441 ccgagccggt ggtgtacatg atggaccgct atgtggtggg tggcttttac cgtatccacg
     1501 cggagcgcgg cgtggacgaa aatctcaatg ccccaggctc cagctatgtt ccattggctt
     1561 ttgctgagag tacgcactta cctcaaccgg gcgagcgccc aggagtcagt gcaccgaacc
     1621 ggttctatat gtacggtgtg gtcgcgcgcc tggccatgct ggcagccagt tacgaactgg
     1681 aagcgaccga cccggacgct gagcactacg actgattttt ggcaagtacg gtgccgatct
     1741 ggagttgctg cgtcgcaaca tagacgcagc aacccaaaaa attgaggatc tgtcctctcg
     1801 ctgttgctgg cgctggcgca aaatggcgat cccagttgga acggactccg gccacgatat
     1861 gtggccggaa taatgtgtgt cagcatctaa atcgtctcta gcggcgctca cgctcggcgc
     1921 catcggcgtg gtctatggcg acattggtac cagtgtcctc tacgcgatga aggaagtgtt
     1981 cgggtcgggc catgtgccct ttacccgcga caacgtcttt ggcattcttt ccatcttctt
     2041 ctggaccctg acggtcatcg tttccatcaa atatgtggtg ttggtgctgc gggccgacaa
     2101 ccacggcgaa ggtggcctgg tggctatgct ggcgctggcc tcgcaatcgg tcaaagatcg
     2161 gccggaactg cgaagagtgc tgctgctggt aggtattttt ggaacctgtc tgttttatgg
     2221 cgatggggtc atcacgccgg caatctcggt gctgggtgct gtcgaagggt tggaggtgat
     2281 ctcgcccacg ttcaagaaat ttgtgatccc cctgacgctg gtcattctgc tggccctgtt
     2341 catggtccag aagcatggca ctgccagcat gggccggttc ttcggcccca tcatggtggt
     2401 gtggttcgcg gtgattgccg gtttgggcct cgtcaacatt gcggcggacc cgctcattct
     2461 ggctgccatc agcccacatt acgcggtggg attcatgtgg gacaacccgg gcaccacctt
     2521 catcatcctg ggagcggtgg tcctgtgtgt caccggcgga gaggccttgt atgcggacat
     2581 gggccacttt ggcaagaagc ccatccgggt ggcctggttc atggtggcca tgcccgcgct
     2641 gacgctgaac tattttggcc agggggcctt gttgctgcac aaccccgcag cagtgaaaaa
     2701 tccgttcttc atgatggcgc cggactgggc cctcattccg ctggttggac tggctgccat
     2761 ggccgccgtg attgcctcgc aggcgctgat ctccggcgcg ttttctgtga ccaagcaggt
     2821 gatacagctg ggctatttgc cgcgcttgca gatccagcac accagcgtga aagacaccgg
     2881 gcagatctac ctgccctttg tgaactgggg cctgtttgcc ctgatcgtgg ttgcggtggt
     2941 gatgttcaag tcctccagta acctggctgc ggcctatggc atcgcggtgt gtacggacat
     3001 gctgatcacc acggtactta ccttttatgt catccgttac agctggaagt acccgctggc
     3061 gctatgcatt ggcgccacag gattcttctt tatcgtcgac tttgcattct gggcgtccaa
     3121 cctgctcaaa ctgtttgaag gtggctggtt cccgctcatg atcggcgggg ccatctttac
     3181 cctgatgctg acctggaaag atggccgacg cctgctcaat gaaaagcttc gggcggactc
     3241 gctggatctg ccgagctttc tggaagccgt attcgtcagc ccgccggcac gcgttgaggg
     3301 cacagcagta ttcctgacgg cagatgtggg tacggtgccc aatgcgatgc tgcacaacct
     3361 caaacacaac aaggttctgc acgtcaacaa cctgttcgtc accgtgcgca accatgaggt
     3421 gccctggatt ggtctggaca agcgaatcga gattgattcg ctgggccacg actgctggca
     3481 ggtggttatc cattacggct tcaagaacga cccggatgtg cccaaggcac tgcagcagat
     3541 caaggggcgg ggctgcgaac tcgaccccat gtccaccagc tatttcctgt cgcgtgacac
     3601 ggtggtaccc accctgggcg aaggcatggc gcagtggcgc gagaagctgt ttgcgcagat
     3661 gcaccacaac gccagtggcg ctgcggactt cctgaacctg cccaacaact cggtggtgga
     3721 gctcggctcc aagatcgaaa tctagtagaa aaactgccca tggatatcct gtttgttgcc
     3781 gacccgctgg agtcgttcaa gatctacaaa gacaccacgt tctccatgat gcgtgaggcc
     3841 cagcgccgcg gccatcgcat cattgcgtgt gagcccaggc atctgatctg gcaaaagggc
     3901 ggtgtggtgc gcgcgcatgt gcgccacatc acgctgaccg gccacgagga gcgctggttt
     3961 cacgaaacgc ccatgccgcc cgagagtcgc ctgcaagcgt tgtatgactt cgatgccatc
     4021 gtcatgcgca aggacccgcc atttgacagc gagtttttct atgccacgca cctgctggag
     4081 caggccgagc gggaaggggc caaggtcttc aacaagccac gcgctttgcg cgatcacccc
     4141 gaaaaactgg ccattctgga atttccccag ttcattggcc cgaccctggt gacgcgcgat
     4201 gcggatgccg tgcgggcctt ccatgccgaa caccgcgaca tcatcctcaa gccgctcgac
     4261 ggcatgggtg gcatgggcat tttccgtgtc aaggacgatg gcctgaacct gggcggcatc
     4321 atcgaaaccc tgaacaagga tggtgcacag accctgatgg tgcagaagtt cctgcccgcc
     4381 attgcgcagg gcgataaacg cgtgctggtg atcggcggca agccggtgcc cttttgcctg
     4441 gcccgtattc cgcagggcgg ggaggtgcgt ggtaatctgg ccgcgggcgg caagggcgtg
     4501 gcgcagccgc taacggatgc ggaccgcgcc atcgcggagg ccttagggcc ggttctggct
     4561 gcgcgcgggc tgctgctcgt ggggctggac atcatcggcg acagcctgac cgaaatcaat
     4621 gttaccagcc ctacctgctt tcaggaaatc acggatcaga ccgggtttga cgtgccggcc
     4681 atgtttgtgg atgcgctgga agccgcgttg taatatttcc acctgaaagt tttgactttc
     4741 ctcaaacctg gatcaaactg aagacacatc caagggaatt gtgtcagtat ggcggtttgg
     4801 cacgatgcca tgccaagact ggttttgtca ccaaaggagt atttatgaag aaagcattac
     4861 tgctggccgc ttcggtcgca ttcatcgccc cccaggcttt tgcacaagcg aaaaacttcg
     4921 aaggtttcag cgtcggcgcc aatctggcgg tggccaagtc gacgacggaa cccactggtg
     4981 ttgcttccga gagcggcaat accaacagtc tcgacctgca agcccagtat tccctggcgc
     5041 tggcaccgca gtttgtgctg ggtttgggcg tgaccctggg gaccggcaac aacaatgcag
     5101 ggactttttc ccttactggt acggaagttt ccaccaagaa ccgtactgcg tttgacatca
     5161 ttcctggtta cgctatttcc aatagcatga tgctgttcgg caaggtatct gcattgagcg
     5221 cagacggcgc gtccaccact gcgggcgtgg aaaccaagaa aagtatttct ggtgtgggtt
     5281 atggctttgg tctgcgcggc ctgatcgaca agaacatgtt ctaccagctg ggctatgaca
     5341 tcaacaagta caacgatgtt gactttggca ctggcaatgt aaaagcgtcc tccagtgtgt
     5401 tctccctggg cgtgggctac aagttctgac tgcggcggat ctgaggttca agaggctcac
     5461 cctggtgggc ctttttcttt gcagtgacct gtcaacaggc tgcaactttt tgcgttattc
     5521 aacaggaggt attcacatga aattcaaagt tctattaacc gcgcttgccg tggcttctct
     5581 ttccaccctg tccgctcagg cgcaggttct gggggcgatt acagcgtccc ccatgaccgc
     5641 caagacgggt gagcctgtca ccattactgc caatatcgat gtactcaacg ccaactactg
     5701 tggtttcgtg gtgggtttcg gtgacggcgc attcaaagat gctgtcagcg acgccagcaa
     5761 ccccaccccg ctggtgctga cccacaccta tgccaaggca ggtaattacc atgtgaccct
     5821 gggtgggaaa aatgtgcaga gccatcccaa ctgcggtggt caggagcgtg cggtggatat
     5881 cgtggtcacg ggcacaacca agtctgcggc acctgctgct ttgaaggcag cggaagtatg
     5941 tgcttccggc tggaagctct cgggcaagct caatgccaag acgggtgctt tcacctgcgc
     6001 cgccaaagcg ggaacagcgt ttcctgctgc caagccggag tgcagtggcg acctgagcta
     6061 tttcgagaac accaagaagg gacagtacgg ctgcaagccg taacttgacg cgggcgtttg
     6121 gcccgctgat gctttatgcc gtttaagagg ctgccagggt tgcgactttg gcagcctctt
     6181 ctgcttcgcg ctgggcagca gcggctgcag cggctgcgat cagcgtggtt ttgcacaccg
     6241 cacgggcaag cggccgccca tccacaaagg cctgcagttc gcccggctcc atggcgaccc
     6301 aggcctcatt ggtggtgagc ggctcggtga ccacaatcac ctggcggtcc tgcgggccgt
     6361 tgaggtcgga gaggtcgacg gtcatgtcct cgtccgcaag cctcacctcc gggaacgggt
     6421 gctggcgcag cacgtagtgc aacttggtgc tggcgtgggc ccagagcgcc tgtccgttac
     6481 tgagcaggaa attgaaggtg ccgtgccgtg cgatgcgcgg caccagctca gacaaggtgc
     6541 aggtgagttc ttccaccgtg ggcacgttgg catgcgactt gttaagctcc tgcagcagcc
     6601 agcagaacgc cagctcgctg tcggtagtgc ccaccggttt gaagctgcca tgtaggggtg
     6661 ggttgaattc cttgaggtcg ccgttgtggg caaacactca gtaacggccc cagagctcgc
     6721 gggtgaatgg gtggcagttt tccagtgtca ccgcaccgac cgtggccttg cgcacatggg
     6781 ccaccacgtt gtggcttttg atggggtagg acttgagcat cttcgcgatc ggcgaggtgg
     6841 atgcgctttc cttgtccaca aagtgccggg ccgctttgcc gggttgatcg ccttcgctct
     6901 caaagaacgc gatgccccag ccatcggagt ggtggtctgt gcagccgcca cgctgcgaaa
     6961 agccggtaaa gctcagcgtc aggctggctg gcaaatagct gttcatccca agcagttggc
     7021 acatgaaaac agtgtaaccc tgcgagggct ctaactgcgg ggttctgacc acagtttgcc
     7081 gcccaccgcc cagttctcgc gccggacctc ggcgataacg acatgcacgc tgtcggggct
     7141 tccgcccaga acctcgacgc tgacacgcgt aatttcctcg gccaattttt tcttctgttc
     7201 cagtgtgcgg ccttccagca tttcaacgtg gtagatgggc atgggcatcc ttttggggaa
     7261 agagtgaata cggttgcagt ttagactgcg gggattgtat ttttcggact gcttcctgtt
     7321 gcgcactgac tccacttttc tgccccgctc accagcgctg cgttggctcc tgctggcgct
     7381 gtttgcgctg cacctgtgtc tgggttcgct catggggctg tcggtcgatg aggcgcatta
     7441 cgccttgtac gcagcacacc ctgcgctgag ttattttgac caccccccgc tggtgggctg
     7501 ggcgcaaatg ccgctggtgg cactgcaggt gcccgacagc gcgctgcggc tggtacccgg
     7561 tctgttgtgg ctggccactg tgctcggggt gtaccgcgtg accgtccagc tgcacgtcga
     7621 gagccagagc gctggcgctg cggcggccgg gttgtgggcc gtactggcgc tggcgctggc
     7681 gccgctgcta cacatcctgg gcatggggtt gttgccggac accctgctga tggcgctgac
     7741 ggtcgctctg atgcaccaga cgctcacgct gacagagttg tcccggcctg ccaggcccat
     7801 cgactggctg gtactcggtg ttctgctcgg gctggcgggc ctgagcaagt acacggccgt
     7861 tcttgcggcc atggctgtgg cgatctgtct gctggctgca catggaccca ggctgttgcg
     7921 cagcccctgg ctgtggctgt cggcgctggt ggcggcgatt ctgattgcac ctgtgatcct
     7981 ctggaatgcc cagaaccagt gggtgtcgtt tgcgtaccag ttccagcatg gtgcgggagg
     8041 agcttggcaa ttcccgcatg tactgcggtt tcttctgctg caagtggtgg tgtatgggcc
     8101 gctgctgctg tggggcgggc agggtctgcg catggtcgcg cggaccccgt cccgttggtt
     8161 ggcggcgttc ttcgcgattc cctttgccct gctggcctac ctgtcaggcg gcggcaccag
     8221 cctgccacac tggacggctc cggcctgggt ggcgctggcg ccgtttgccg gcatggcact
     8281 ggcacacgcg gtccagaccc gggcgcgcaa atgggtcctg gccctggtgg ctgtgcaggc
     8341 gctgttgtgc ctcgcattac ccgttttgat ggccacgggt ggtgtgcctt tcttccaggc
     8401 caaggctgcc accagtgcgc aagctcccaa tccttttgct gatctgcatg gctgggacca
     8461 ggccggggcc cgcgcacgca gcctggcggc gcaacaccag ctcgacagcg tatccgtgca
     8521 gaactggacg ctggccagcc gtctgggctg gtacgctcga cccatgaaag tgcatgtgct
     8581 cgaagaccgt tttgaccagt ttgacctgtg ggccggtgat ctgccgcagg gaggcaatac
     8641 gctgctggtc gactggagtg aaatggccta tgtgacaccc gtgggcggca atggctttgc
     8701 aagctgcaag ttgctggacg tattgcctgt gcagcgcctg ggccggccgc tgtccgagtt
     8761 tcgcttctat gcctgcaatg gctggtccgc agaccccaaa ccacgcctgc agtccgaggc
     8821 gggtgcgcca tgacatcggc gcagttcaac aaggatgtgg cgctctgggt ctgggctttg
     8881 cctgtagtgc tgttcctggt gtctgcgccg ctgtggctgc actggtggga gccacagatg
     8941 tttgtagcca tcaacaccct gtgcatgcct gtggcagcgc agttctggac cgggttgtcg
     9001 ctgctgggca atggctgggg catcttgggg gtcaccgcgc cgctgctggt gctggcgccc
     9061 cgactgatgt gggcctggct gt
//
LOCUS       DAOSJM010000046         9051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7273_length_9051_cov_4.870831, whole genome shotgun sequence.
ACCESSION   DAOSJM010000046 DAOSJM010000000
VERSION     DAOSJM010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9051
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7273_length_9051_cov_4.870831"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PKX82_02775"
     CDS             <1..259
                     /locus_tag="PKX82_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-anti-sigma factor"
                     /protein_id="HOF51200.1"
                     /translation="VTVLVSVAGWGVWNTTSDGQQLASTRGVPVIAQMEVAAGSAEGP
                     VMLRDPELDALMAAHQQLGGHSALQMPSGFLRNATFERPAR"
     gene            256..1254
                     /locus_tag="PKX82_02780"
     CDS             256..1254
                     /locus_tag="PKX82_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HOF51201.1"
                     /translation="MSQGKHTVAWVLAATALLWCGVGLAAESAVADTSIHAWLVRVHE
                     ASRQRAYTGTFVVSAGAKTASAKIWHVCDGTQQMERVEPLSGQSRSIFRRNDQVITFF
                     PESRVAITEKRDSLGLFPNLLKSSDSDIGAHYQLKALGNERVVGVEADIVEILPKDQL
                     RYGYRVWSEKRTGLIVQLQTLDLEGRVLEQAAFSELQLDAPVSMPKLTQMMAATDGYR
                     IESPEMQKTTAEVQGWTLRRQVPGFRAMGCFLRPVATSAQGVHAMQWMFSDGLATVSL
                     FVEPFDRRRHTREGSTDLGGATRTLTRHLDAWWITAVGEVPAATLGVFAQTLERRK"
     gene            1295..2776
                     /locus_tag="PKX82_02785"
     CDS             1295..2776
                     /locus_tag="PKX82_02785"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOF51202.1"
                     /translation="MFTMAFLTMKPVRSLFVAAAVAVTAVAAMVPVQPAMAQARGLPD
                     FTDLVDQVGPSVVNIRTMEKISARPAQGGTLGEEEMLEFFKRFGLPIPNVPRQAPRQN
                     RPQQPDEEQPRGVGSGFILSADGLIMTNAHVVEGADEVLVTLTDKREFKAKIIGADKR
                     TDVALVKIEAAGLPAVRVGDVNRLRVGEWVMAIGSPFGLENTVTAGIVSAKQRDTGDY
                     LPFIQTDVAINPGNSGGPLINMRGEVVGINSQIYSRSGGFMGISFAIPMDEAVRVSDQ
                     LRSAGRVSRGRIGVQIDQVSKDVAESIGLGKPIGAMVRGVEAGGPAEKAGVEAGDIIT
                     RFDGKVIDKSSDLPRIVGSTKPGNRSTITVFRRGSYKDLNVTVAEVEADRPVAAKGAG
                     REDKPKGSVAGQAFGLTVAEITDAQKKELAVKGGVRVEAALDAAARAGLRDGDIILQI
                     GNSAVLNVKEFEVAIGKHDKSKPLNVLFRRGDWTQYAVIRPNR"
     gene            3268..5079
                     /gene="lepA"
                     /locus_tag="PKX82_02790"
     CDS             3268..5079
                     /gene="lepA"
                     /locus_tag="PKX82_02790"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOF51203.1"
                     /translation="MNHIRNFSIIAHIDHGKSTLADRLIQRCGGLEAREMEAQVLDSM
                     DIEKERGITIKAQTAALKYKALDGKVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLNVEVLPVLNKIDLPNADPDNARTEIEDVIGIDATDAIV
                     CSAKTGLGIDDILEAIVAKIPSPKGNPEGPLRAMIIDSWFDSYVGVVMLVRVVDGRLA
                     KGERIKMMASGAMYNADNLGVFTPANQPRESLEAGEVGYIIAGIKELQAAKVGDTVTL
                     IKPGTGGAAFTATEPLPGFKEIQPQVFAGLYPTEASEYDSLRDALEKLKLNDASLHYE
                     PEVSQALGFGFRCGFLGLLHMEIVQERLEREFDQDLITTAPSVVYQVVKNDGEVVMVE
                     NPSKMPEVGKTAEIREPIVTVHLYMPQEYVGVVMTLANQKRGIQMNMAYKGRQVVLTY
                     EMPLGEIVLDFFDKLKSVSRGYASMDYEFKEFRASDVVKVDILLNGDKVDALSIIVHR
                     SQSQYRGRAVVGKMREIISRQMYDVAIQAAIGANIIARETIKALRKNVLAKCYGGDIS
                     RKKKLLEKQKAGKKRMKQIGSVEVPQEAFLAILQVED"
     gene            5079..6047
                     /gene="lepB"
                     /locus_tag="PKX82_02795"
     CDS             5079..6047
                     /gene="lepB"
                     /locus_tag="PKX82_02795"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOF51204.1"
                     /translation="MQALTGAVLAAFGLYAASWYFGMVEGNFALLLLLATVVTGVYWL
                     AERFYFLPQRQQAAAALEANDIARRAELAKMGIAQVDGDITEAKHRLLMQPWWLDWTA
                     GLFPVIIAVFVLRSFLFEPFKIPSGSMIPTLLVGDLILVNKFTYGLRLPVINTKITQG
                     NAPQRGDVMVFRYPPKPSLDYIKRVVGVPGDTVAYLNKRLTVNGKAIETQAIPEFFDE
                     DAMRYFKQYEESLGERKHRLLNDDERPAFVMPMPDFQQFRENCSYTVEGMTCKVPEGH
                     YFMMGDNRDNSQDSRYWGFVPEKNIVGRAFFVWMNFGNLKRIGSFD"
     gene            6061..6414
                     /locus_tag="PKX82_02800"
     CDS             6061..6414
                     /locus_tag="PKX82_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HOF51205.1"
                     /translation="MTVQIRSRQRGISFIGLVVVGSLLAMAGVVAAQIFPTVLELQAI
                     QKAVNKASEGNSVVEVRTIFDKQASIDDIKSISGKDIEVTKEGDKVVVAFSYQREIHL
                     AGPGYLTLKYAGRSK"
     gene            6431..7102
                     /gene="rnc"
                     /locus_tag="PKX82_02805"
     CDS             6431..7102
                     /gene="rnc"
                     /locus_tag="PKX82_02805"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOF51206.1"
                     /translation="MELQRRLQHEFSDPALLSRALTHRSFSSDHYERLEFLGDSVLSL
                     AVSDLLYSRLGSLPEGDLSRVRANLVKQDTLHQLAVDLGLPDVLKLGEGEVRSGGQKR
                     PSILADALEAIIGAVYLDAGFAKAQSLVHRLFNAVEINPQMEAIGKDPKTELQEWLQG
                     RKMKLPVYTVVGTIGAAHKQSFDVECEIPQLQLSERGIGGSRRAGEQAAAAAMLQTIK
                     SRNPS"
     gene            7183..8142
                     /gene="era"
                     /locus_tag="PKX82_02810"
     CDS             7183..8142
                     /gene="era"
                     /locus_tag="PKX82_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOF51207.1"
                     /translation="MLEGLLKSTPKLAQPGAVAEGQRCGLVAIVGKPNVGKSTLLNAL
                     VGQKISITSRKAQTTRHRITGMRTQGPTQFVFVDTPGFQTLHGNALNKSLNKTVVGAV
                     ADVDLILFVVEAGSFTPADARVLSLLGKDIPTLLIANKLDNVHRRGDIAPWLQTMQTK
                     HAFAEFVPMSAKSAKDIERLLTICEKYLPEQAWWYSEDELTDRSEKFLASELVREKLF
                     RLTGDELPYTSTVVIDKFEEEPPQKKGQKRLLRIAATIVVERDGHKAMVIGDKGERIK
                     RIGMETRVELEKLANAKVFLELWVKVRSGWADDEARVRSFGYE"
     gene            8249..8998
                     /gene="recO"
                     /locus_tag="PKX82_02815"
     CDS             8249..8998
                     /gene="recO"
                     /locus_tag="PKX82_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOF51208.1"
                     /translation="MATKRIADEPAYVLHRYDWSESSLILEVFTRHYGRIALVAKGAK
                     RPSSSFRPILLPMQPLHVAFGGDAEIRTLKSAEWQGGHVMPTGDALLSGYYLNELLMT
                     LLARDDPHAELFDIYARVVQIIASEHGEVLQAALRTYELLLLREIGFLPQLNVQTMTL
                     APVEPDVHYVLVPEGGLRQAHADDRASLSGVQWMRLHDALQDNAPFTTTLRACAELMS
                     NLKPQLRALLHYHCGVKTLRTRQMMIDIQAL"
BASE COUNT         1877 a         2532 c         2813 g         1829 t
ORIGIN      
        1 cgtgacggtg ctggtatcag tcgcgggatg gggcgtatgg aatacgacgt cggatggaca
       61 gcagctggcg tccacccggg gggtgccggt gatcgcgcag atggaagtgg ctgcagggtc
      121 agcagagggc cctgtcatgc tccgtgatcc tgagctggac gccctgatgg ctgcgcacca
      181 gcaattggga gggcattccg cattgcagat gccatcgggc ttcctgcgca acgccacatt
      241 cgagaggccc gcacggtgag ccagggaaaa cacaccgtcg cgtgggtatt ggcagccacc
      301 gcgctgctgt ggtgtggcgt cgggttggcc gcagagagtg ctgtggcgga taccagcatc
      361 catgcatggt tggtgcgtgt gcatgaagct tcaaggcagc gcgcctacac gggcaccttt
      421 gtagtgtccg ccggtgccaa gacggcgagt gccaaaatct ggcacgtctg cgatggaacg
      481 cagcagatgg agcgcgtgga accactcagc ggccagtcgc gctccatatt ccgccgcaac
      541 gaccaggtga ttactttttt cccggagtcc cgtgtggcca tcacggagaa gcgggattcc
      601 ctgggcctgt ttcccaacct gctcaagtcc agcgatagcg atataggtgc gcactaccag
      661 ctcaaggcat tgggcaatga gcgcgtggtc ggagtcgagg cggacatcgt agaaatcctc
      721 cccaaggacc agctgcgtta cggataccgt gtctggagcg agaagcgcac gggattgatc
      781 gtgcagctac aaacgctgga tctggaggga cgtgtactgg aacaggccgc cttctcggag
      841 ttgcaactgg atgcaccggt cagcatgccg aagctgacac agatgatggc cgcgaccgac
      901 gggtaccgca ttgagagccc ggagatgcag aagaccacag ccgaagtgca aggctggacc
      961 ttgcggcggc aagttcccgg tttcagggcc atgggttgct tcctgcgccc ggtcgcaaca
     1021 tcggcgcaag gtgtccatgc catgcagtgg atgttttcgg acgggctggc aacggtgtct
     1081 ctgtttgtgg aaccctttga caggcggcgc cacacccgcg aaggctctac cgatctgggg
     1141 ggggcgacgc ggaccttgac ccgccatctg gatgcgtggt ggatcacggc ggtcggcgaa
     1201 gttcccgctg cgacgttggg tgtttttgcg cagacccttg agcgcaggaa atgaggcacc
     1261 acatggtgct tgttgttctt tcttagatgg atggatgttc acaatggcat ttttgacgat
     1321 gaaacccgtg cggtcacttt tcgtggcagc tgctgtggcg gtcacggccg tggcggcgat
     1381 ggttccggta cagcctgcaa tggcccaggc gcgggggctg ccggacttta ccgatctggt
     1441 cgatcaggtg ggcccttccg tggtcaacat ccggaccatg gagaaaatca gtgcgcgacc
     1501 agctcagggt ggcaccctgg gagaagagga gatgctggag tttttcaagc gatttggtct
     1561 tccgattccc aatgttccac gccaggcgcc acgccagaac cgtccccagc agccggacga
     1621 agagcaacca cggggtgtgg ggtcgggctt cattttgtcg gcagacggcc tgatcatgac
     1681 caatgcccat gtggtggagg gtgccgatga ggttctggtg acactgaccg acaagcgaga
     1741 gttcaaagcc aagatcattg gtgcggacaa gcgtaccgat gtcgcactgg tcaagatcga
     1801 ggcagcgggc ttgcctgcgg tcagggtggg cgatgtcaac cgtctgcgtg tgggcgaatg
     1861 ggtgatggcg atcggctcac cgtttggctt ggagaacaca gtgacggcag gcattgtgag
     1921 tgccaaacag cgtgatactg gcgattacct tccattcatc cagaccgatg tagctatcaa
     1981 tcccggcaat tcgggtggtc cgctgatcaa tatgcgcggc gaggtagtgg gtatcaacag
     2041 ccagatttac tcccgctctg gcggcttcat gggaatttcg tttgcgatcc ccatggacga
     2101 ggcagtgcgc gtgagcgacc agttacgcag tgcagggcgc gtatcgcgcg ggcgcattgg
     2161 cgtgcagatt gaccaggtca gcaaggacgt cgcggagtcc atcgggctgg gtaagcccat
     2221 cggtgccatg gtgcgtggtg tggaagctgg cggaccggca gagaaggccg gtgtggaagc
     2281 gggagacatc attaccaggt ttgatggcaa ggtgatcgac aagtccagcg acttgccccg
     2341 catcgttggc agcaccaaac ctggcaaccg cagcacgatc acggtctttc gccgtggcag
     2401 ctacaaggat ttgaatgtga cggtggctga ggtagaggca gacaggcccg tggcagccaa
     2461 aggcgctggt cgcgaagaca aacccaaggg ttctgttgcc ggccaggcat tcggtttgac
     2521 ggttgcggag atcacggatg cgcaaaagaa ggagcttgcc gtcaagggtg gcgtgcgggt
     2581 tgaagctgcg ctggatgccg ctgcccgggc tggcttgcgc gatggcgaca tcattttgca
     2641 gattggcaat agcgcggtcc ttaatgtgaa agagtttgag gtggcgattg gcaagcatga
     2701 caagagcaag ccgctcaatg tgctgttccg ccgtggagac tggacgcagt acgccgtcat
     2761 tcgccccaac cggtaagaag gcgacttccc ggtgacaacc ccaaaccctt gtggggcttg
     2821 gggttgtttt ttggagtcaa aaaaaattat ctgcggacgg gaggccggtt cattacttat
     2881 gtatgcaacc gctaactttt tggactgcct tgccttattg gttagaatac ggggtcgcat
     2941 cagctcatta tgggcataag gccagtccaa aagtccatca tatgggatgg tttcgggtgg
     3001 cccacaggcg atccacagat cgcccacaag ttgtccagaa ggtatgcggc attatttgtt
     3061 tataagctgt gcataagttt gctgttgtgg actcgcaacg acacgtctta ccgaattttg
     3121 gggctgtgaa cccgggactt tttgcctaca atgaagttcc aactttcgca gttgccaaaa
     3181 gattgttggt tcagggcgcg tccacaacag acgcgccctt ttttctgctc gcctcttttt
     3241 aattcaatca cttgaagctc ttcgttgatg aatcacatca gaaatttttc gatcattgcg
     3301 cacattgacc atggcaaatc cactcttgcg gatcgcttga tacagcgttg cggtggactg
     3361 gaagcgcgtg agatggaggc gcaggttctg gactcgatgg atatcgagaa agagcgtggg
     3421 ataaccatca aggcgcagac ggctgcgctc aaatacaagg ccctcgatgg caaggtctac
     3481 aacctgaacc ttatcgacac gccggggcat gtggacttct catatgaagt gagccgatcg
     3541 ctgtccgcgt gtgaaggcgc gctgctggtc gtggatgcgt cgcaaggtgt ggaagcgcag
     3601 acggtggcca attgctacac cgcacttgac ctgaatgtgg aagtgctgcc agtactgaac
     3661 aagatcgacc tgcccaacgc cgaccccgac aacgcacgca cagaaattga ggatgtgatc
     3721 gggatcgacg ccacggacgc gattgtgtgt tctgccaaga cagggctggg cattgatgac
     3781 attctggaag ccatcgttgc aaagatccct tcgcccaaag gcaatccgga gggccccctg
     3841 cgcgccatga tcatcgacag ctggtttgac agctatgtgg gcgtagtgat gctggtgcgt
     3901 gtagtcgatg gccgtctggc caaaggcgag cgcatcaaga tgatggcgtc cggtgcgatg
     3961 tacaacgccg ataacctggg agtgttcacc cccgccaacc agccgcgcga gtcgctggaa
     4021 gcgggtgagg tgggctacat cattgccggt atcaaggagc tgcaggcggc caaggtgggt
     4081 gacaccgtca ccctgatcaa gcccggaacg ggcggcgctg ccttcactgc caccgagccg
     4141 ctgcccggtt tcaaggaaat ccagccgcag gtgtttgccg gtctgtatcc gactgaggcc
     4201 agcgaatacg actcgctgcg cgacgcgctg gaaaagctca agctcaacga tgcctccctg
     4261 cactacgagc ccgaagtgtc ccaggcgctg ggcttcgggt tccgttgcgg cttcctgggc
     4321 ttgctgcaca tggagatcgt gcaggagcgt ctggagcgcg agtttgacca ggacctcatc
     4381 accacggcac cgagcgtggt gtaccaggtg gtcaagaacg atggcgaagt cgtcatggtg
     4441 gagaacccct ccaagatgcc ggaagtgggc aagaccgcag aaatccgtga gccgatcgtc
     4501 acggtgcacc tctacatgcc gcaggaatat gtgggtgtgg tgatgacact ggccaaccag
     4561 aagcgtggta tccagatgaa catggcctac aagggccgcc aggtcgtgct gacttacgag
     4621 atgccactgg gcgagatcgt gctggacttc ttcgacaagc tcaagagcgt gagccggggt
     4681 tatgcgtcca tggactacga gttcaaggag ttccgtgctt ccgatgtggt gaaggtcgac
     4741 atcctgctca acggcgacaa ggtggatgca ctgtccatca tcgtgcaccg cagccagtcg
     4801 cagtaccgcg gccgcgccgt ggtgggcaag atgcgcgaga tcatttcacg gcagatgtat
     4861 gacgtagcca ttcaggccgc catcggtgcc aacatcattg cgcgtgagac aatcaaggcc
     4921 ctgcgcaaga acgtgttggc caagtgctat ggcggtgata tttcgcgcaa gaagaagctc
     4981 cttgagaaac aaaaagcagg caaaaaacgc atgaaacaga tcggttcggt ggaagtcccc
     5041 caagaggcgt tcctggccat tttgcaggtg gaagattgat gcaagctctc actggcgccg
     5101 tacttgcggc tttcggcctg tacgccgcat cctggtattt cggcatggtg gagggcaatt
     5161 tcgccttgct gctgttgctc gccacagtcg tcacaggtgt gtattggctc gccgagcgct
     5221 tctacttctt gcctcaacgc cagcaggcgg ctgcggcgct ggaagcgaat gacattgccc
     5281 gccgtgccga gctcgccaag atgggcattg cccaagtgga cggtgacatc accgaggcca
     5341 agcatcgtct gctcatgcag ccctggtggc tggactggac cgcaggactg ttcccggtga
     5401 tcatcgccgt gttcgtgctg cgctctttcc tgtttgagcc gttcaagatt ccttcgggct
     5461 ccatgattcc aaccctgctg gtgggcgacc tgattctggt caacaagttc acctatggtt
     5521 tgcgtctgcc ggtgatcaat accaaaatca cccagggcaa tgcgccgcaa cgcggcgatg
     5581 tcatggtgtt tcgctacccg cccaagccca gcctggacta catcaagcgc gtggtgggtg
     5641 tcccgggcga tacggtggct tacctgaaca agcggctgac cgtgaatggc aaagcgatcg
     5701 aaacccaagc catccccgag tttttcgatg aagacgccat gcgctacttc aagcagtacg
     5761 aggaatcgct cggggagagg aagcaccgtc tcctcaatga cgatgaacgt ccggcctttg
     5821 tcatgcccat gccggacttc cagcagttcc gcgagaactg cagctacacc gtggaaggga
     5881 tgacctgcaa ggttcccgaa ggccactatt tcatgatggg cgacaatcgc gataattcgc
     5941 aggattcccg ttactggggc tttgtgccag agaaaaacat cgtgggccgg gcgttctttg
     6001 tctggatgaa ctttggcaac ctcaagcgga tcggttcgtt tgactgaaag ggacataaag
     6061 atgacagtgc aaatcaggtc gcgccagcga ggtatttcgt ttatcggatt ggtggtcgtg
     6121 ggctccttgc tggcgatggc cggtgtggtg gctgcacaga tctttcctac ggtactcgaa
     6181 ctgcaggcta tccagaaagc ggtcaataag gcatccgaag gcaactcggt ggtggaagtg
     6241 cgcaccattt ttgataaaca ggcgtccatt gacgacatca agtcgatttc cggcaaggac
     6301 atcgaagtca ccaaagaggg tgacaaggtg gtggtggcgt tcagctacca gcgcgaaatt
     6361 caccttgccg gcccaggcta cctgacgctc aaatacgcag ggcgatccaa ataacgtggc
     6421 agatgctctg atggagttgc agcgcaggct gcagcatgaa ttttctgatc ccgccctgct
     6481 gtccagggct ctgacccatc ggagcttttc ttcggaccac tacgagaggc tcgagtttct
     6541 cggcgattcg gtgctcagtc ttgccgtctc cgatttgctg tattcccggc tgggcagtct
     6601 gcccgaaggg gatctgtccc gcgtgcgcgc caatctggtc aagcaggaca ccctgcacca
     6661 gctcgctgtc gatttggggc tgcccgatgt cctcaagctc ggcgagggtg aagtgcgctc
     6721 gggcgggcag aagcgcccct ccatcctggc cgacgcgctg gaagccatca tcggtgcggt
     6781 ctacctggac gccggttttg ccaaggcgca gtccctggtg caccggctgt tcaatgcggt
     6841 ggagatcaat ccgcagatgg aggccattgg caaggacccc aagaccgagt tgcaggaatg
     6901 gctgcagggg cgcaaaatga aattgccggt gtacaccgtc gtcggcacca tcggtgcggc
     6961 gcacaagcag agcttcgatg tggagtgcga aattccccag ctgcaattgt ccgaacgcgg
     7021 catcggaggc tcccgccgtg cgggcgaaca ggccgccgcc gcggccatgc tgcaaaccat
     7081 caaatccaga aatccatcat gaatgctcct aaaaagatag ctgccagcgc agattccacg
     7141 ggggctaatg gtcaaattga gcctcaagac gatctagaaa gcatgctcga aggtctgctc
     7201 aagagcacgc ccaaactggc ccaacccggt gccgtcgccg aaggccagcg ctgcggtctg
     7261 gtcgccatcg tgggcaagcc caacgtcggc aagtccactt tgctcaatgc cctggtggga
     7321 cagaagatca gcatcacctc acgcaaggcc cagactacac gccaccgtat taccggcatg
     7381 cgtacccagg gtcccacgca gtttgtgttt gtcgatacgc ccggtttcca gactttgcat
     7441 ggcaatgcac tgaacaagtc gctcaacaaa accgtggtgg gtgccgtggc cgatgtggat
     7501 ctgatcctgt tcgtggtgga ggcgggtagt tttacccccg cagacgcccg tgtgctgtca
     7561 ttgctgggca aagacattcc cacgctgctg atcgccaaca agctcgacaa cgtgcaccgc
     7621 cgcggcgaca tcgcgccctg gttgcagacc atgcagacca agcacgcgtt cgccgaattt
     7681 gtgcccatgt cggccaagag cgccaaagac atcgaacgcc tgctgaccat ctgcgaaaaa
     7741 tacctgcccg agcaagcctg gtggtattcg gaagacgagc tgaccgatcg cagcgagaag
     7801 tttcttgcca gcgaactggt gcgcgaaaag ctgttccgtc tgaccggtga cgagttgccc
     7861 tacacctcca cggtagtcat cgacaagttc gaggaagagc caccgcagaa gaagggccag
     7921 aagcgcctgc tgcgcattgc cgccacgatt gtggtggagc gcgacggcca caaggccatg
     7981 gtgattggcg acaagggcga gcgcatcaag cgcatcggca tggagacccg ggtcgagctg
     8041 gaaaagctgg ccaacgccaa ggtgtttctg gagctgtggg tgaaagtgcg ctctggctgg
     8101 gccgatgacg aagcacgcgt gcgcagcttc ggctatgaat agtgccccca cgctccgccg
     8161 ctgcgcgggt cgctgccccc caggggggcg cgtttcagct tggggcggcc cggcgctgaa
     8221 acccactctc acgtcctgca cctgagtcgt ggcgaccaaa cgcattgctg acgagcccgc
     8281 gtatgtgctg caccgctacg actggagcga gtccagcctg attctggaag tcttcacccg
     8341 ccactacggg cgtatcgctc tggtggccaa gggcgccaag cggcccagct ccagtttccg
     8401 tcccatcctg ctgcccatgc agcccttgca tgtggccttt ggtggcgatg ccgagatccg
     8461 taccctcaag agcgccgaat ggcagggcgg ccatgtcatg cccaccggcg atgcgctact
     8521 gtcgggctac tacctcaacg aactgctcat gacgctgctg gcgcgcgacg atccgcatgc
     8581 cgagctgttt gatatctacg cccgcgtggt gcagatcatt gccagcgagc atggtgaggt
     8641 gctgcaggcg gcattgcgca cctacgagct gctgctgttg cgtgaaattg gtttcctgcc
     8701 ccagctcaat gtgcagacca tgacgctggc cccggtcgag cccgatgtcc actatgtgct
     8761 ggtacccgaa ggcggcttgc gccaggccca tgccgacgac cgcgccagcc tcagtggcgt
     8821 gcagtggatg cggttgcacg acgcgctgca ggacaacgct cccttcacca ccacactgcg
     8881 tgcctgtgcc gagctcatgt ccaacctcaa accgcagctg cgagcattgc tgcactacca
     8941 ttgcggcgtc aagaccctgc gtacgcgcca gatgatgatc gacattcagg cgctgtaaaa
     9001 aggcttcctc accatgaaaa cccatctctc cgtcaacctc aacaaagtcg c
//
LOCUS       DAOSJM010000047         8908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7408_length_8908_cov_4.463572, whole genome shotgun sequence.
ACCESSION   DAOSJM010000047 DAOSJM010000000
VERSION     DAOSJM010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7408_length_8908_cov_4.463572"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..2893
                     /gene="fdhF"
                     /locus_tag="PKX82_02820"
     CDS             2..2893
                     /gene="fdhF"
                     /locus_tag="PKX82_02820"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOF51209.1"
                     /translation="MLHPPADTTEGTIRFELDDDAVQARPGESIWEAARRHGIEIPHL
                     CHTDGLRPDGNCRACVVEIAGERTLAPSCCRAPTPGMQVRARSPRALKSQEMVLELLL
                     ADLPEQGHKWQDNDPARPHGELSDWADRMGVTPRPALTALRREAPAPDLSHPAMAVNL
                     DACIQCNRCVRACREEQVNDVIGYALRGSHSEIVFDLNDPMGDSTCVACGECVQACPT
                     GALMPKAHVGSQAVDKRVDSVCPFCGVGCLLTYNVKDNRIVSVDGRDGPANHNRLCVK
                     GRFGFDYAHNPQRLTVPLIRKAGVPKDPEAINRLNRDTADWSDVFREATWEEALDLAA
                     GTLRTLRDTHGKKALAGFGSAKGSNEEAYLFQKLVRTGFGSNNVDHCTRLCHASSVAA
                     LLEGVGSGSVSNPVNDVAHSDLILVIGSNPTNNHPVAATWMKNAAKLGARIVVADPRG
                     TDIGRHAWRTLQFKADTDVAMLNALIHTIIEEGLTDQDFIARRANNFEALRENVKGYS
                     PEAMAPICGIPAETLREVARAYAKAKAAMILWGMGVSQHVHGTDNVRCLIALATVAGQ
                     VGKPGSGLHPLRGQNNVQGASDAGLIPMMYPNYQRVSNKAVHDWFENFWGMPLDDQPG
                     YTVVEIMGKILAPDTDPHKVRGMYIMGENPAMSDPNLNHTRHALASLEHMVVQDIFMT
                     ETAWLADVVLPASAWPEKTGTVSNTDRMVQMGKAAIAPPGQARADLWIIQEIASRMGL
                     HWSYEKDSALRNVDGGYHGVELVYEEMRQAMHAAIGGITWERLERESSVTYPCHSADD
                     PGEAIVFHDHFATDDGRVHLVPADIIPANERPDAEFPYVLITGRQLEHWHTGSMTRRA
                     TVLDALEPMATASMCGDDLQKLGLQPGDVMTVQSRRGQVVIRVRRDDGTPAGAVFMPF
                     AYYEAAANLMTNPALDPFGKIPEFKYCAVSVQPGGELVPVAGFSSAPLH"
     gene            2899..3492
                     /locus_tag="PKX82_02825"
     CDS             2899..3492
                     /locus_tag="PKX82_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HOF51210.1"
                     /translation="MAEQEIESSRSDSESTRPGVDVAVVMRRERMDNRWQPWRWVLAD
                     VVQQEEGFGTEPRLLLKSEQEQRWLYPGFRVELLAGDAEGYYLNATTEQPCFWVVWRM
                     EEEATVAEEPLAIPQVVTLSYHDAGRWLDAQETVEQVPAPTSIVEWLRAFVDRHHVVE
                     PKRRQRPQSFQSLQDRFGNPVRFSTEKKGGGGGGHGR"
     gene            3482..4063
                     /locus_tag="PKX82_02830"
     CDS             3482..4063
                     /locus_tag="PKX82_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HOF51211.1"
                     /translation="MADEPSGFLGRWARRKADAREGKPLPEPPRPVKAVELAPRVEPE
                     PASAVAVPQAEPQHEEAPVPSLTLDDARKLNKDSDFTPFMARQVEPEVRNAAMKTLFA
                     DPHFNVMDGLDTYIDDYSKPDPLPLAMLRQMASAKFLNLFEEDPPSPAAIDQSVAQSS
                     SLPPAPPTDHAHTHLRLQPDDAPPAQSAGSGSA"
     gene            3990..6074
                     /locus_tag="PKX82_02835"
     CDS             3990..6074
                     /locus_tag="PKX82_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF51212.1"
                     /translation="MPTLICDCNQTMPLQPKALGAALHEELTLHSTLCRREAGAFQKA
                     LQSGEDVVVACTQEARLFNEIAQQTEGVPAVPIRFVNIRETGGWSRDAAQASPKIAAL
                     LALAHLPDPEPVATVDFKSEGRLLIIGPLDAAEHAAELVADVMDITIFSTGAGSNGGA
                     QERRFPVVSGQLRTLSGWLGAFEVNWTRSNPIDLDLCTRCNACVAACPEGAIGFDYQI
                     DSALCKSHRVCVKVCGVAGAIDFTREPDAQTDRFDLVLDLRATSAFTQHALPQGYFRW
                     DGKDQRTLLNLRALVGEFEKPKFFAYKQKLCAHSRNEKTGCSACIDVCSASAISSERD
                     RQQIKVNPNLCVGCGACTTVCPSGALTYAYPRASDQGVKLKTLLTTYARAGGKDAAVL
                     LHSQEAGARLIGDLGRAARVDAATHGVPARVLPVALWHTASVGLELWLSAVAYGASQV
                     WILMTGEEAPQYQEAVRAQMDVAQAILHGLGYTGEHFRILHARDARDLATLDAQLQAP
                     PAQCVTHPAGFAIQAEKRATLDLAIDHLVTQSRVQPALIPLPSGSPLGALALDKDRCT
                     MCLSCINACPAAALQDNPLAPQLKFIEKNCVQCGLCVKTCPEKALALVPQLRLGAQRK
                     EPVVINEMQPYTCIRCGKPFGTLKAIEAMLGKLAGHSMFQGAAADRLKMCGDCRVIDL
                     YTDPNEVRITDL"
     gene            6099..6710
                     /locus_tag="PKX82_02840"
     CDS             6099..6710
                     /locus_tag="PKX82_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HOF51213.1"
                     /translation="MSPPSDASWDEETARAELYGLLSRLYYAPPDADLMAQLRVAVTE
                     APASGGFLEEPWRALVAAARDIPPQSVADEYELLFGGVGKPEVYLYGSHYLSGFLNEK
                     PLAHLRTTLAELGLERDPSMPETEDHFAYLCEVMRYLIAGDDVAVANLTHQRDFFAQH
                     MQPWAVAMCDAIAGHSQARFYAALAELTRAFLEVESQGFDMLV"
     gene            6817..7029
                     /locus_tag="PKX82_02845"
     CDS             6817..7029
                     /locus_tag="PKX82_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HOF51214.1"
                     /translation="MSQPKNGLSRRTLFAGAGTAGAVAAVAGLAAATSGTTPQALPEL
                     VKPAPEKGGGYSLSEHVKRYYGTARV"
     gene            7061..>8908
                     /locus_tag="PKX82_02850"
     CDS             7061..>8908
                     /locus_tag="PKX82_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOF51215.1"
                     /translation="MLLTKKSPAHQGESAQRTGSTRFVQSLSRGLSNALPTMDRRVFL
                     RRSGLGVGVGLAASQLTLVKKSQAASAAAGDGSGKIEVKRTVCTHCSVGCAVDAVVQN
                     GVWVRQEPVFDSPINLGAHCAKGAALREHGHGEFRLRYPMKLVNGKYERISWDTALDE
                     ISARMLALRKESGPDSLYVVGSSKHNNEQAYLLRKWMSFWGSNNCDHQARICHSTTVA
                     GVANTWGYGAMTNSYNDMQNSKCALYIGSNAAEAHPVSLLHMLHAKETGCKMIVVDPR
                     FTRTAAKADEYIRIRSGSDIPFLFGMIYHIFKNGWEDKKYIQDRVYGMDKLKEEVMSK
                     WTPDKVEEACGVPEARVLMAAEMMAKNRPSTIVWCMGQTQHTIGNAIVRASCILQLVL
                     GNVGVSGGGTNIFRGHDNVQGATDVGPNPDSLPGYYGLAEGSWKHFAKTWGVEFDWIK
                     GRYASPAMMGKSGITVSRWIDGVMEKNELIDQDSNLRGLFFWGHAPNSQTRGLEMKRA
                     MDMLDLLVVVDPYPSATAAMAAMPGKPEDLNPNRAVYLLPAATQFETSGSCTASNRSI
                     QWREKVIEPLWESRSDHMIMQQFADRLGFGKELSKNYKMQKVKGMDEPVP"
BASE COUNT         1724 a         2728 c         2824 g         1632 t
ORIGIN      
        1 cctgctgcat ccccccgccg acacgaccga aggaaccatc cgcttcgagc tcgatgacga
       61 tgccgtgcag gcccgccccg gcgagtccat ctgggaggca gcacgccgcc acggcatcga
      121 gattccgcac ctgtgccaca ccgatggtct gcgccccgat ggcaattgcc gtgcttgtgt
      181 ggtcgagatc gctggcgaac gtacgctggc gccgagttgc tgccgtgcgc ccacaccggg
      241 catgcaggtg cgtgcgcgca gcccgcgtgc gctcaagagc caggaaatgg tgctggagtt
      301 gctgcttgcc gatctgccag agcaagggca caagtggcag gacaacgatc ccgcgcgccc
      361 gcacggcgaa ctcagcgact gggccgaccg catgggcgtg acgccgcgtc cggccctgac
      421 ggctctgcgc cgtgaagcgc ctgcaccgga cctgtcccac ccagcgatgg cagtgaatct
      481 ggatgcctgc atccagtgca accgctgtgt gcgggcctgc cgtgaagagc aggtcaacga
      541 tgtcatcggc tacgccctgc gcggtagcca cagcgagatc gtgttcgacc tgaacgaccc
      601 catgggcgac agcacctgcg tggcgtgcgg cgaatgcgtg caggcctgcc ctacgggcgc
      661 cctgatgccg aaggcccatg tgggcagcca ggcggtcgac aaaagagtgg actctgtctg
      721 tccgttctgt ggtgtgggtt gtctgctgac ctacaacgtc aaggacaacc ggattgtgag
      781 cgtggacggc cgcgatggcc cggccaacca caaccgcctg tgcgtcaagg ggcgtttcgg
      841 gtttgactat gcgcataacc cgcagcgtct gacggtgcca ttgatccgca aggccggtgt
      901 gcccaaagac ccggaagcca tcaaccgcct gaatcgggac accgcagact ggtctgacgt
      961 gtttcgtgaa gccacctggg aagaggccct cgatctggcg gcgggcacgc tcaggacctt
     1021 gcgcgacaca cacggcaaga aggctctggc cggctttggt tctgccaagg gcagcaacga
     1081 agaggcatac ctgttccaga aactggtgcg caccgggttt ggcagcaata acgtggacca
     1141 ttgcacccgc ttgtgccatg cctccagcgt ggctgcctta ctcgaagggg tgggctctgg
     1201 ctcggtgagc aatccggtca acgatgtggc ccattccgac ctgatcttgg tgatcggctc
     1261 caacccgacc aacaaccatc cggtggctgc gacctggatg aaaaacgctg cgaagctggg
     1321 ggcccgcatc gtggtggccg acccgcgcgg aaccgatatc ggacggcatg cctggcgcac
     1381 cctgcagttc aaggccgaca ccgatgtggc catgctcaac gcgctgatcc acaccatcat
     1441 tgaagaaggc ctgaccgacc aggacttcat tgcccgtcgt gccaacaatt tcgaagccct
     1501 gcgcgagaac gtcaagggct acagccccga ggccatggcc cctatttgcg gcattcccgc
     1561 ggagaccttg cgcgaagttg cgcgggccta cgccaaagcg aaggctgcga tgattctgtg
     1621 gggcatgggg gttagtcagc acgtgcatgg caccgacaac gtgcgctgcc tgatcgccct
     1681 ggccacggtg gccgggcagg tgggcaagcc gggttcgggc ctgcatccgc tgcgcggcca
     1741 gaacaatgtg cagggcgcat ccgatgcggg gctgattccg atgatgtacc ccaactacca
     1801 gcgggtgtcg aacaaggcgg tccacgactg gttcgagaac ttctggggca tgccgctgga
     1861 cgaccagcct ggctacaccg tggtggagat catgggcaag atcctggcgc ccgataccga
     1921 tccccacaag gtgcgcggca tgtacatcat gggtgagaac ccggccatga gcgaccccaa
     1981 cctcaaccac acccgccatg cgctggccag cctggagcac atggtggtgc aggacatctt
     2041 catgaccgaa accgcgtggc tggccgacgt ggtgctgccc gcatcggcct ggccggaaaa
     2101 gacaggcacc gtgagcaaca ccgaccgcat ggtgcagatg ggcaaggccg ccattgcgcc
     2161 acccgggcag gcccgtgcgg acctgtggat catccaggaa atagccagtc gcatgggttt
     2221 gcactggagc tatgaaaaag atagcgcatt gcgtaatgtg gacgggggct accacggtgt
     2281 ggaacttgtc tacgaggaga tgcgccaggc catgcacgca gccatcggcg gcatcacctg
     2341 ggagcggctg gaacgcgagt ccagcgtcac ctacccttgc cacagtgcag acgatccggg
     2401 cgaggcgatt gtgttccatg accatttcgc caccgacgat ggccgtgtgc atctggtgcc
     2461 agccgacatc atcccggcca acgaaaggcc agacgccgaa tttccgtatg tgctgatcac
     2521 cggccgccag ctggagcact ggcataccgg tagcatgacg cgccgcgcca cggtgctcga
     2581 tgcactggag cccatggcta ctgcttcgat gtgtggggac gatctgcaga agctgggttt
     2641 gcagcccggt gacgtgatga ccgtgcaatc gcgccgcggg caggtggtca tccgggtgcg
     2701 acgcgacgac ggcacccctg ccggtgcggt gttcatgccc tttgcgtatt acgaggcggc
     2761 cgccaacctg atgaccaatc ctgcgctgga cccgtttggc aagattccgg agttcaagta
     2821 ctgcgccgta tcggtgcaac ccggtggcga actggtgccg gttgcagggt tttcgtccgc
     2881 gcccctacac tgagggctat ggcagaacaa gaaatcgaat cctcgcgcag cgacagcgag
     2941 tccacccgcc ctggcgtgga tgtcgccgtg gtcatgcgcc gagagcgcat ggacaaccgc
     3001 tggcaaccct ggcgctgggt gctggccgac gtggtgcagc aggaagaagg ctttggcacc
     3061 gaaccgcggc tactgctcaa gagcgaacag gaacagcgct ggctgtatcc cgggttccgc
     3121 gtggaactcc ttgcgggcga cgccgaaggt tattacctga atgccaccac cgaacaaccg
     3181 tgcttctggg tcgtctggcg catggaagaa gaggccactg tggccgagga acctctggcc
     3241 atcccgcagg tggtgacact gagctaccac gacgcaggtc gctggctgga tgcgcaggaa
     3301 accgtggagc aggtcccggc tccgacttcc atcgtggaat ggctgcgcgc ctttgtggac
     3361 cgacaccatg tggtggagcc caagcgccgg cagcgccccc aaagcttcca gtccctgcag
     3421 gaccgttttg ggaatccggt gcgtttttcc accgagaaga agggtggagg agggggcggc
     3481 catggccgat gaaccttcgg gtttcctcgg gcgctgggca cgccgcaagg ccgatgcgcg
     3541 cgaaggcaag cctttgcctg agcccccgcg tccggtcaag gcggtggagc tggcgccacg
     3601 tgtggagcca gagcccgcca gcgcagttgc cgtgccgcag gctgaaccgc agcatgagga
     3661 agcgccagtt ccatcgctga cgctcgatga tgcacgcaag ctgaacaagg actccgattt
     3721 cacgccgttc atggcgcggc aggtagagcc cgaggtgcgc aatgcagcga tgaagacgct
     3781 gttcgccgat ccgcatttca atgtgatgga tggtctggac acctatatag acgactactc
     3841 caaaccggac ccgctcccgc ttgccatgct gcgccagatg gcgagcgcca agtttctcaa
     3901 tctctttgaa gaagatcccc cgagccctgc ggcgatagac caatccgtgg cacagtcttc
     3961 atctttgccc cctgcaccac ctactgacca tgcccacact catttgcgac tgcaaccaga
     4021 cgatgcccct ccagcccaaa gcgctgggag cggctctgca tgaagagctg acgctgcatt
     4081 ccaccctgtg ccgccgcgag gctggcgctt tccagaaagc gctgcagtct ggtgaagacg
     4141 tggtggtggc ttgcactcag gaagcgcggc ttttcaacga aattgcgcag cagaccgagg
     4201 gggtgccggc cgttcccatc cgctttgtca atatccgcga gaccggcggc tggagtcgcg
     4261 atgccgcaca ggccagtccg aagattgccg ccctgctggc actggcgcat ttgcccgacc
     4321 cggagccggt cgcgacggtg gacttcaaga gtgaagggcg cctgctgatc atcgggccac
     4381 tggacgcggc cgaacacgcc gccgaactgg tggccgacgt gatggacatc acgatcttca
     4441 gcacgggcgc gggcagcaac ggtggcgctc aggagcgccg gtttccggtg gtctccggcc
     4501 agttgcgaac gctgtcgggc tggctgggtg cgtttgaggt gaactggacc cgaagcaacc
     4561 cgatcgacct ggacctgtgc acccgctgca atgcctgtgt ggccgcctgc ccggagggag
     4621 ctatcgggtt tgactaccaa attgatagcg ctttgtgcaa gtcccacagg gtctgcgtca
     4681 aggtttgtgg tgtggccggt gcgattgact tcacccgcga gccggatgcc cagaccgacc
     4741 ggtttgatct ggtgctcgac ttgcgggcca cttcagcgtt cacccagcat gcgttgccgc
     4801 agggctattt ccgctgggac gggaaggacc agcgtacgct gctcaacctg cgcgcactgg
     4861 tgggcgagtt cgaaaagccg aagttctttg cctacaagca gaagctctgt gcccacagcc
     4921 gcaacgaaaa aacaggttgc agtgcctgta tcgatgtgtg ttctgcttcc gcgatcagca
     4981 gcgagcggga tcgccagcag atcaaggtca atcccaacct gtgtgtggga tgtggcgcct
     5041 gcaccaccgt ctgccccagc ggggcgctga cttacgccta cccgcgtgcc agtgaccagg
     5101 gcgtgaagct caaaaccctg ttgaccacgt atgcccgcgc tggtggcaaa gatgccgccg
     5161 tgctgctgca cagccaagag gcgggcgcgc ggttgattgg agacctgggg cgcgcggcgc
     5221 gagtggatgc ggcaactcac ggtgtaccgg cacgcgtgct gcctgtcgcg ctgtggcaca
     5281 ccgcctcggt agggttggag ttgtggctgt ctgccgtggc ctatggtgcc tcgcaggtgt
     5341 ggattctcat gaccggagag gaggctccgc aataccagga ggcggtgcgt gcccagatgg
     5401 acgtggccca ggccatcctc cacggcttgg gctacacggg cgagcatttc cgcattcttc
     5461 acgccaggga tgcgcgtgac ctggccacgc tggatgccca gttgcaggca ccaccggccc
     5521 agtgcgtcac ccatccggcc ggatttgcca ttcaggcgga aaagcgcgcc acgctggatc
     5581 tggcgatcga ccatctggtg acgcagtcca gggtgcaacc cgcgctgatt cccctgccca
     5641 gcggttcccc actgggtgcg ctggcgctgg acaaggaccg ctgcaccatg tgcctgagct
     5701 gcatcaacgc ctgtcctgca gcagcgttgc aggacaaccc gctggcgccg caactcaagt
     5761 tcattgaaaa gaattgtgtg cagtgcgggt tgtgcgtcaa gacctgcccc gagaaagcac
     5821 tggccctggt gccgcagctg cgtctgggtg cacagcgcaa ggaaccggtc gtcatcaacg
     5881 agatgcaacc ctacacctgc atccgctgtg gaaagccttt tggaaccctg aaggccatcg
     5941 aggccatgct ggggaaactg gcagggcaca gcatgttcca gggtgctgcc gccgaccgac
     6001 tcaagatgtg cggtgactgc cgggtgatcg acctgtacac cgaccccaat gaagtccgca
     6061 ttaccgacct gtaaagaatg accatgacat ccgcatccat gtccccacct tccgacgctt
     6121 catgggacga agagaccgcc cgcgccgagt tgtacggact cctgtcgcgt ctgtactatg
     6181 ccccgccgga tgccgatctg atggcccagc tgcgtgttgc ggtgacagaa gctcctgcgt
     6241 ccggcggctt tctggaggaa ccctggcgcg ccttggtggc ggctgcgcgc gatattcctc
     6301 cccaatccgt tgccgatgaa tacgaactgc tgttcggcgg cgtgggcaag cccgaggtct
     6361 acctctatgg ttcgcattac ctgagtggtt ttctgaacga aaagccactg gcccacctgc
     6421 gtaccaccct ggctgagttg ggcctggaac gtgacccgtc catgccggaa accgaggacc
     6481 actttgccta cctgtgcgag gtcatgcgct acctgattgc cggagacgat gtggccgtag
     6541 ccaatttgac acaccagcgc gatttttttg cgcagcacat gcagccctgg gcggtagcga
     6601 tgtgcgatgc aattgcgggg cattcccagg ccaggttcta tgccgcgctg gcagagctga
     6661 cgcgggcgtt tctggaggtg gagtctcagg ggtttgacat gctggtgtga gggtgcgggt
     6721 tagcaacact tgccgaaacg tcaggagatt gttaggctct ggcgcataca ctacatccta
     6781 tgcatgttca tgcctaggtg aataaggaga cccaccatga gtcaacccaa aaacggtttg
     6841 tcccgccgca ccctgtttgc cggtgcaggc accgcaggtg ctgtggctgc cgtagccgga
     6901 ctggcggcgg ccacgtccgg aacgaccccg caggccctgc cggagctggt caagccagcg
     6961 cctgaaaaag gtggcggtta cagcctgagc gaacacgtca agcgctacta cggcaccgcc
     7021 cgcgtttaag ttcttttcca ttccctcaac ggagagccgc atgctgctca cgaaaaaatc
     7081 cccagcccat cagggtgagt cggcccaacg cactggatcg acacgttttg tacaaagtct
     7141 ctcgcgtggc ctgtccaacg cattgcccac catggatcgc cgggttttcc tgcgtcggtc
     7201 cggcctgggc gtgggtgttg gcctggccgc gagccagctg acgctggtca aaaagtccca
     7261 ggctgcgtct gccgcagcgg gggacgggtc tggcaagatt gaagtcaaac gcaccgtctg
     7321 cacgcactgc tcggtgggtt gcgcggtgga cgctgtcgta caaaacggcg tgtgggtccg
     7381 tcaggaaccg gtgtttgatt ctcctatcaa cctcggtgcg cattgcgcca agggtgcggc
     7441 gctgcgtgaa cacggccatg gcgaattccg gctgcgttac cccatgaagc tggtcaatgg
     7501 caaatacgag cgcatcagct gggatactgc actggacgag atttcggccc gcatgcttgc
     7561 gctgcgcaag gagtccggcc ccgattcgct gtatgtcgtg ggctcctcca agcacaataa
     7621 cgagcaggcc tatctgctgc gcaaatggat gagcttctgg ggtagcaaca actgcgatca
     7681 ccaggcacgt atttgtcact ccaccacggt agcgggtgtg gccaatacct ggggttatgg
     7741 cgccatgacc aattcgtaca acgacatgca gaacagcaag tgcgcgttgt acatcggctc
     7801 caatgcggca gaagcccatc cggtcagcct gctgcacatg ctgcatgcca aggaaaccgg
     7861 ttgcaagatg atcgtggtcg atcctcgttt cacgcgtacg gcggccaagg ccgacgaata
     7921 catccgtatc cggtccggtt cggacattcc gttcctgttc ggaatgattt atcacatctt
     7981 caagaacggg tgggaagaca aaaagtacat ccaagaccgt gtgtacggca tggacaagct
     8041 caaggaagag gtcatgtcca agtggacacc ggacaaggtc gaagaggcct gcggtgtacc
     8101 tgaggcgcgt gtcttgatgg ccgcggagat gatggccaag aaccgcccct ccaccattgt
     8161 ctggtgcatg ggccaaaccc agcacactat cggcaatgcc atcgtccgtg cctcctgcat
     8221 ccttcagctg gtgttgggca atgtgggagt ttccggtggc ggcaccaaca tcttccgtgg
     8281 gcacgacaac gtacagggcg ccaccgatgt gggccccaac cccgactccc tgccgggcta
     8341 ctatggactg gctgaaggct cgtggaagca ctttgccaag acctggggcg tggagtttga
     8401 ctggatcaag ggtcgttacg cgtctcctgc catgatgggc aaatccggaa tcaccgtgtc
     8461 gcgctggatt gatggcgtga tggagaagaa cgagctgatt gaccaggact cgaacctgcg
     8521 gggtctgttc ttctgggggc atgctcccaa ttcgcagacc cgtggtctgg aaatgaagcg
     8581 ggccatggac atgctggatc tgctggtggt ggtggacccc tatccatcag ccaccgcagc
     8641 gatggccgcc atgcccggca agccggaaga tctcaacccc aaccgggcgg tgtacctgct
     8701 gccggctgct acccagtttg aaaccagtgg ctcgtgtacg gcatccaacc gttccattca
     8761 gtggcgcgaa aaagtcattg aaccgctgtg ggaaagccgc agcgaccaca tgatcatgca
     8821 gcagttcgca gacaggctgg ggttcggcaa ggaattgtcc aagaactaca agatgcagaa
     8881 ggtcaagggc atggacgagc ccgtgccc
//
LOCUS       DAOSJM010000048         8775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7521_length_8775_cov_4.731193, whole genome shotgun sequence.
ACCESSION   DAOSJM010000048 DAOSJM010000000
VERSION     DAOSJM010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7521_length_8775_cov_4.731193"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PKX82_02855"
     CDS             <1..221
                     /locus_tag="PKX82_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOF51216.1"
                     /translation="FGIIMVVNMEVGMCHPPVGLNLYVASGITKMGITELTVAVWPWL
                     LSMLGFLMVVTYWPGLTLWFPRMLGMVP"
     gene            complement(243..740)
                     /locus_tag="PKX82_02860"
     CDS             complement(243..740)
                     /locus_tag="PKX82_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOF51217.1"
                     /translation="MTTPRTVMESYAVLRVGAAEVAQHRDGLTLLAPIPVALARLGLF
                     DRSKVPAPEDYATTSQVADFESKLATTPGFFWMVTDTNDRVTQVNAGRAYVRMQLAAT
                     AQGLGMQPLQQALQEYVEQKGPYTGIHQQLAAPGQTVQMWARVGYGPEVEPAPRRRLE
                     DFVRA"
     gene            complement(737..1267)
                     /locus_tag="PKX82_02865"
     CDS             complement(737..1267)
                     /locus_tag="PKX82_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51218.1"
                     /translation="MQRRSFVRLVGGGTVAAATLGSAGLAGCSAAMPSEAIEAWRGPG
                     ADMLSGDVRRWMLSYAILAPHSHNLQSWVVDLRTPGEMVLYCDPKRLLPQTDPYARQI
                     MMSHGTFLELLQIAACERGLRAEVVLFPDGAFGATELDGRPVARIRCMWGWWGRRIPA
                     RWPHTARLPWRPGVSS"
     gene            1377..2279
                     /locus_tag="PKX82_02870"
     CDS             1377..2279
                     /locus_tag="PKX82_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF51219.1"
                     /translation="MHWFDEFETRHLDVNGVQIYARVSPGLPADRPALLLLHGFPQTH
                     VMWHRVAQLLHKDFYLVMPDLRGYGDSSKAPGLPDHSTYSKRSMAQDMVDVMDVLGVT
                     RFGVCGHDRGGRVAHRLALDHPTRVSGLCVIDIAPTLDMYAATSFAFAQAYYHWFHLT
                     QPEPLPETMIGGNALAYLHHKLGGWGTGGMAHIEPAALAEYERCFQKPETIHAVCEDY
                     RASASIDLEHDRTSRAKGDRIACDTLVLWGDRGVVQRLFNPMALWQAQCAAHVTGQAM
                     PAGHFIPEELPLQTARLLTDFFAR"
     gene            complement(2306..3106)
                     /locus_tag="PKX82_02875"
     CDS             complement(2306..3106)
                     /locus_tag="PKX82_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HOF51220.1"
                     /translation="MSTNSPSTVVIAPLSPEEFDEIDAILDDLRTRFEETPQWEFCEG
                     FMAALVCGRRAMDPEEYLPVLLDTGDGVAGELGAFADAAQAARFMALWHRRWNDVAQG
                     LAAEVQSLDDERAYHPEVMDVRGAVAALPEAERASMDGERLPSFAQVWAVGFMYAVES
                     WPEEWVAPRDKDAQKWLDGALEAIVALTEDDTDEPTLSVFEEDGPPSVSVKRFNTFAD
                     AVWAVYDLRELWRNVGPRVETVRKEAEPGRNDPCPCGSGKKYKKCHGA"
     gene            3161..4789
                     /locus_tag="PKX82_02880"
     CDS             3161..4789
                     /locus_tag="PKX82_02880"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HOF51221.1"
                     /translation="MLHYLNTLFPIRYTVFGLSCFGLLLSLFSLLAFGAGLGALVLFG
                     FLTGIGIYDVRQDKRSVLRNYPIIGHIRYMLEFVRPEIRQYFIESDKEAAPFSRAQRS
                     LVYQRAKGEPDKQPFGTQLDVHAEGYEWMNHSVSPTSLTTHDFRVTVGADRAQPYSMS
                     VFNISAMSFGALSANAILALNGGAKRGGFAHDTGEGSISQHHRVHGGDLVWEIGSGYF
                     GCRNDDGTFNAEKFAANARDPQVRMIEIKLSQGAKPGHGGVLPGPKVTAEIAAARGVP
                     EGVDCVSPASHSAFSTPVELMQFIEQLRTLSGGKPTGFKLCIGHPWEWFAMVKAMLST
                     GITPDFIVVDGAEGGTGAAPVEFTDHVGSPLQEGLLLVHNTLRGVNLREKIKIGCAGK
                     VVSAFDIARLMAIGADWCNSARGFMFALGCIQSAHCNTGACPTGVTTQDPLRQQSLVV
                     PDKMERVYNFHQQTLHALLEMVQAAGLQHPQDITAHHIVRRGTDHKVKSLAQLILTQL
                     GSGALLKDDLSPLPLIYQHTWPLANAESFAIARS"
     gene            complement(4883..5539)
                     /locus_tag="PKX82_02885"
     CDS             complement(4883..5539)
                     /locus_tag="PKX82_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HOF51222.1"
                     /translation="MAKAVLTISSKNYGAWALRGWLAARLAGLDFTEEVISPDDPAMK
                     AEMLLLSSSMLVPSLHHNGITVWDTLAIGEYLNEIKPKAHLLPADAAARAHCRAICGE
                     MHSGFASLRSSLPMNIKARFPNFKVWSRAQADIDRIVAIWKECLATYGGPYLFGKQPC
                     MADAMYAPVVTRLLTYDVALDKVCAAYCKRIMELPAMQEWVKEAKKEPDDIDELDAEF
                     "
     gene            5679..6899
                     /gene="phaZ"
                     /locus_tag="PKX82_02890"
     CDS             5679..6899
                     /gene="phaZ"
                     /locus_tag="PKX82_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HOF51223.1"
                     /translation="MLYQAYQLQSDLLSPFRLFAQGGAAALWMHNTENSLLRKVSASM
                     DVFSRLRLTHSRPPYAIASVTVGEREFPVIEETVLGMPFGTLLRFKKDNAQDLPYQPP
                     VLLVAPLSGHFATLLRETAKTLLQDHDVYITDWHNARDVSLRHGPFGLDDYIDHMIRF
                     TQAIGPGTHVVAVCQPCVAALAATAIMAEDDDPAQPLSLTLMAGPVDCRVNPTEVNKL
                     ATSKPIEWFEKNLISHVPLPHKGFMRRVYPGFVQVSAFMAMNPERHKQSFRDMYRHLV
                     DGDIAQADTIRTFYDEYLAVNDLPAEFYLETVEKVFQTYDLPLGKLQYRGRTVNPAAI
                     RRTALMTVEGERDDICAVGQTVAAQDLCSSIRPYRKTHHIQTGVGHYGVFSGRRWNQQ
                     IYPRVRDMIHASVG"
     gene            complement(6921..8252)
                     /locus_tag="PKX82_02895"
     CDS             complement(6921..8252)
                     /locus_tag="PKX82_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOF51224.1"
                     /translation="MKAIKTSWVVLLLGLSLLWLWADAPAWYAMQGVFAWRNEMLQYT
                     GVLGIGVMSVAMVLAIRPVALEHRLHGLDKMYRLHKWLGITGLVVAIAHWLWVEAPKW
                     LVQAGWIARPVRGPRPAPSADFAGFLQGQRGLAEGVGEWAFYASLVLIAIALVKYFPY
                     RHFFKTHRWMAVVYLALVLHSVVLMRFSYWSTPLGVVMASLMLCGTVAAVISLFRRIG
                     SRRKAVGVIGTLETLDGVGVSAVTVQLKSAWPGHQAGQFAFVSFDAREGAHPYTISSD
                     WKDDGQLQFLIKALGDYTRTLTSTLKPGGVVEVEGPYGRFNFEGTSPRQIWIGGGIGI
                     SPFVARMKALAANPDGREVDLFHTTADVDEVALQKLRDDARNAGVRLHILVDARDGRL
                     TGDRLRTAVPDWRVADVWFCGPTAFGDAIRQDLVSQGLPALNFHQELFAMR"
     gene            8390..>8775
                     /locus_tag="PKX82_02900"
     CDS             8390..>8775
                     /locus_tag="PKX82_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOF51225.1"
                     /translation="MIRIQLNIELNYEIDQFGADFVFNIHAAHTASQQISSENLFLSQ
                     AIDPQIYTDPVNGNRYMRLRAWPGPLKVQYSATVDLTHHFSNPAQVPEVPVRNLPPEV
                     MGYIYPSRYCQSDRLLKLANSTFGGQ"
BASE COUNT         1738 a         2899 c         2519 g         1619 t
ORIGIN      
        1 acttcggcat catcatggtg gtgaacatgg aagtcggcat gtgccaccca ccggtgggct
       61 tgaatctgta cgttgcctcg ggcattacca agatggggat caccgagctc acggtggcgg
      121 tctggccctg gctgctgtcg atgctgggct tcctgatggt ggtgacctac tggccgggtc
      181 tgacactgtg gtttccgcgc atgctgggaa tggtgcccta acagcatggt ggaccggaat
      241 gctcaggcgc ggacgaaatc ttccagtctg cgtcggggtg ccggttccac ttcaggtcca
      301 tagcccacgc gtgcccacat ctgcaccgtc tggcccggtg cagccagctg ttggtggatg
      361 ccggtgtaag gccccttctg ttccacatac tcctgcagtg cctgctgcag cggctgcatc
      421 cccagcccct gcgccgtggc agccagttgc atacggacat aggcgcgacc cgcgttaacc
      481 tgggtgaccc ggtcattggt atcggtcacc atccagaaga atcccggagt ggtggccagt
      541 ttcgactcga agtccgccac ctggctggtg gttgcgtaat cctccggggc gggtaccttg
      601 ctgcggtcaa acagccccaa tctggccagg gccaccggta tcggtgcaag caaggtcagc
      661 ccgtcgcggt gttgcgccac ttcggcagct cccacgcgta acacagcata agactccatc
      721 actgtgcggg gtgttgtcaa ctcgatacgc caggcctcca gggcaatctg gcggtgtgtg
      781 gccagcgcgc cgggattctc cggccccacc aaccccacat gcaccggatc cgagccaccg
      841 gccggccatc caattcggtt gcaccaaacg caccgtcagg aaacagcacg acctctgcac
      901 gcaacccgcg ttcacaggcc gcgatttgca ggagttcgag aaacgtacca tggctcatca
      961 tgatctgacg tgcatacggg tccgtctgcg gcaggagtcg cttcgggtcg cagtacagca
     1021 ccatctcgcc aggcgtgcgc aaatccacta cccacgattg caggttatgt gaatggggtg
     1081 ccaggatggc ataactcagc atccagcgac gtacatcgcc ggacagcatg tccgcaccag
     1141 gaccacgcca cgcctcgatc gcttcggacg gcatcgctgc gctgcacccc gccaatccag
     1201 cagaccccag cgtggccgct gcgacggtac cgccacccac caggcgaaca aagctacgtc
     1261 gttgcatggg gctctccatt tcacctgttg atggatgcat tgtcagctga acatgccatt
     1321 gtctgaacac acgtgatcta atttgcaaca ccttcaagac gccaaaggac cccgccatgc
     1381 actggtttga tgagtttgaa acgcgccacc tggatgtcaa cggtgtgcag atctacgccc
     1441 gtgtttcgcc ggggctgccg gcagaccgtc cggccctttt gctgctccac ggttttccgc
     1501 aaacccacgt gatgtggcac cgcgtcgccc agcttctgca caaggacttc tacctggtga
     1561 tgccggatct gcgcggctat ggcgactcca gcaaggctcc gggcctgccc gaccacagca
     1621 cctacagcaa acgatccatg gcgcaggaca tggtcgacgt gatggatgtt ctgggcgtaa
     1681 cgcgctttgg cgtctgtggc catgaccgcg gtgggcgggt agcgcaccgc ctggctctgg
     1741 accaccccac gcgggtgagc gggctgtgtg tgatcgacat cgcccccaca ctggacatgt
     1801 atgcggccac cagcttcgcg tttgcccagg cctactacca ctggttccat ctgacccaac
     1861 ccgaaccctt gccggaaacc atgattggcg gcaacgcgct ggcctatctg caccacaaac
     1921 tgggcggctg ggggaccggc gggatggcac acattgaacc cgccgcgctg gccgagtacg
     1981 aacgctgctt ccagaagcca gaaaccatcc atgctgtgtg tgaggactac cgggccagtg
     2041 ccagcattga cctcgaacac gaccggacaa gtcgtgcaaa aggcgaccgg attgcctgcg
     2101 acacgctggt tctatggggc gaccgtggcg tagtgcaacg gctcttcaac ccgatggcgt
     2161 tatggcaagc acagtgtgcg gcccacgtca cggggcaggc catgccggcc ggccacttta
     2221 tccccgagga attgccacta cagacagccc gcctgctgac ggacttcttc gcgcgctagc
     2281 cgctttttgc aacggcccac ggcggttacg cgccgtggca cttcttgtac ttcttgccac
     2341 tgccgcatgg gcaaggatcg ttgcgacccg gctccgcttc ctttcggacc gtttccacac
     2401 ggggaccgac attgcgccag agttcgcgca ggtcgtagac cgcccacacc gcatcggcaa
     2461 aagtgttgaa ccgtttgaca ctcacgctgg gcgggccatc ttcttcaaac accgacagcg
     2521 tcggctcatc ggtatcgtcc tcggtcagcg ccacaatggc ttccagcgcg ccgtcgagcc
     2581 atttctgtgc gtccttgtca cgcggagcca cccactcttc gggccagctc tctaccgcat
     2641 acatgaagcc cacggcccac acctgcgcaa acgaaggtag cctttccccg tccatgctgg
     2701 cacgctccgc ctcgggcagc gctgccaccg caccacgcac atccatcacc tcggggtggt
     2761 aggcgcgctc gtcatccagc gactgcacct ccgccgcgag gccctgcgcc acatcgttcc
     2821 agcgtcgatg ccagagcgcc atgaatcgcg ccgcctgcgc tgcatcggca aatgcgccca
     2881 actcccccgc gacgccgtcg ccagtgtcca gcaataccgg caaatattct tccgggtcca
     2941 tggccctgcg gccacataca agtgctgcca tgaaaccctc acaaaactcc cactggggcg
     3001 tttcctcaaa tcgggtgcgc aggtcgtcaa ggatggcatc gatttcgtca aactcctcgg
     3061 gactcagagg cgcaatgaca acagtggagg gtgaattcgt agacataggg tgtgcaaaat
     3121 ggttggtggc gtgtattgtc accccgaagg aaaacccacc atgctgcatt acctgaacac
     3181 cctgttcccg attcgctaca ccgtctttgg actcagctgt tttggactgc tgctcagcct
     3241 cttcagcctg ctggcttttg gcgcaggact gggcgcattg gtgctgttcg gatttctcac
     3301 aggcatcggc atctatgacg tgcgccagga caaacgttcc gtattgcgca actaccccat
     3361 cattggccac atccgctaca tgctggagtt tgtgcgcccg gagattcgcc agtacttcat
     3421 cgaaagcgac aaggaggccg cgccgttttc ccgcgcgcag cgctcactgg tgtaccagcg
     3481 cgccaagggt gagcccgaca agcagccctt cggcacgcag cttgatgtgc atgctgaagg
     3541 ctacgaatgg atgaaccatt ccgtctctcc caccagtctg accacccatg acttcagggt
     3601 gactgtgggt gcggaccgcg cacaacccta ttccatgagt gtgttcaaca tctctgccat
     3661 gagctttggc gcactgagtg ccaatgccat tctggcgctc aatggtggtg ccaaacgcgg
     3721 cggctttgcc catgacaccg gcgagggctc catctcgcaa caccaccgcg tgcatggtgg
     3781 cgatctggtc tgggaaatcg gttcgggcta cttcggctgc cgtaacgacg atggcacatt
     3841 caacgccgag aagtttgctg ccaatgcccg tgacccccag gtccggatga ttgaaatcaa
     3901 gctgagccag ggcgccaagc ccggccatgg cggtgtgctg cccggcccca aggtcaccgc
     3961 ggagatcgct gcggcccggg gtgtgccaga aggcgtggac tgcgtgtcgc cagcatccca
     4021 cagcgccttc agcacgccag tggaattgat gcagttcatc gagcagctgc gcacgctgtc
     4081 tggtggcaag cccacgggat tcaagctgtg catcggtcac ccctgggaat ggtttgccat
     4141 ggtcaaagcc atgctgtcca ccggcatcac accggacttc atcgtggtcg acggtgccga
     4201 agggggcacg ggcgcagccc cggtggaatt cacggaccat gtgggttctc cgctgcagga
     4261 agggctgctg ctggtgcaca acaccctgcg tggcgtcaac ctgcgcgaaa aaatcaagat
     4321 tggctgtgcc ggcaaggtcg tgagtgcctt cgacattgcc cggctgatgg ccattggcgc
     4381 cgactggtgc aacagcgcgc gcggtttcat gtttgctttg ggctgcatcc agtcagcgca
     4441 ttgcaatacc ggcgcctgcc ctaccggtgt gaccacacag gacccgctgc gccagcagtc
     4501 gctggtggtg cctgacaaga tggagcgcgt gtacaacttt caccagcaga cgctgcacgc
     4561 gctgctggaa atggtgcagg cagccggcct gcagcacccg caggacatta ccgcccacca
     4621 tatcgtgcgc cgcggcaccg accacaaggt caagtcactg gcccagctca tcctcaccca
     4681 actgggcagc ggggccttgc tcaaggatga cctgagtcct ttgcccctga tctaccagca
     4741 cacctggccg ctagccaacg cggagagctt tgccatcgcc agaagctaac cccaccggcc
     4801 aagtcaggca gggaagcacc agaaccatgc aaagccaggc acgagcacag ggcgcgtcgg
     4861 ctactgccaa ttcagacgcg ggctagaact cggcgtcgag ttcgtcgata tcgtccggct
     4921 ctttcttcgc ctccttcacc cactcctgca tggcaggcag ctccatgatg cgtttgcaat
     4981 aggccgcgca caccttgtcc agcgccacgt cgtaggtcag gagccgggtc accaccggcg
     5041 catacatggc gtccgccatg cacggctgct tgccaaacag gtacggaccg ccgtaggtgg
     5101 ccaggcactc tttccagatg gccacgatgc ggtcaatgtc cgcctgcgcg cgggaccaga
     5161 ccttgaagtt cggaaagcgg gctttgatgt tcatcggcag cgaggaacgc agcgaggcga
     5221 atcccgagtg catttcgccg caaatcgccc ggcagtgcgc ccgagccgcg gcatcggcag
     5281 gcagcaaatg ggccttgggt ttgatctcgt tgaggtattc accgatggcc agcgtatccc
     5341 agacggtgat gccgttgtgg tgcagcgatg gcaccagcat gctgctggag agcagcagca
     5401 tctcggcctt catggccggg tcatccgggg aaatcacctc ctcggtgaaa tccagtcccg
     5461 ccagccgggc cgcgagccag ccgcgcaggg cccacgcgcc atagttttta ctgctgatgg
     5521 tcaatacggc ttttgccatg gtggtgctcc tcaaaccaga aacgtcacgg ggatagccgc
     5581 aagggccgtg ccaacaagaa gtggcccgca aattgcctgc agtcagggca ccacgcccag
     5641 tcacgcacca ggagtgccca tttctaccgg agaaacctat gctgtaccag gcctaccaac
     5701 tgcagagcga cctgctgtcg ccctttcggc tttttgcaca agggggtgct gccgccctgt
     5761 ggatgcacaa cacggaaaac agcctgctgc gtaaagtgtc tgcctcgatg gacgtgtttt
     5821 cccggctgcg gctgacgcac tcacgcccgc cgtacgccat cgcgtccgtc actgtcggtg
     5881 agcgcgagtt tccggtcatc gaagagacgg ttctgggcat gccatttggc acattgttgc
     5941 gcttcaaaaa ggacaacgcg caggacttgc cttaccagcc cccggtgctg ctggtggcgc
     6001 ccctgtcagg ccacttcgcc accctgttgc gcgagaccgc caagacgctg ttacaggacc
     6061 atgatgtcta tatcaccgac tggcacaatg cgcgcgacgt atcactgcgc catgggccgt
     6121 tcggactgga cgactatatc gaccacatga tccgcttcac gcaggccatt ggtccgggca
     6181 cgcatgtggt ggcggtgtgc cagccgtgcg tggcggcatt ggcagccacc gccatcatgg
     6241 ccgaagacga tgacccggcc cagcctttga gcctgacgct gatggctggc ccggtggact
     6301 gtcgggtcaa cccgaccgaa gtcaacaagc tggccaccag caaacccatc gagtggtttg
     6361 aaaagaacct gatcagccat gtgccgctgc cccacaaagg cttcatgcgc cgcgtgtacc
     6421 cgggctttgt gcaggtcagc gcgttcatgg caatgaatcc ggaacggcac aaacagtcct
     6481 tccgggacat gtaccgccat ctggtcgacg gtgatattgc ccaggccgac accattcgca
     6541 ccttctacga cgagtacctg gcggtcaacg acctgccggc cgagttctat ctggaaaccg
     6601 tggagaaggt attccagacc tacgacctgc cgctgggcaa gctgcagtac cgcggccgca
     6661 ctgtcaatcc tgccgccatc cgtcgcaccg ccttgatgac ggtggagggt gagcgcgatg
     6721 acatctgcgc cgtaggacag acggtggcgg cgcaagacct gtgcagcagc atccgcccgt
     6781 ataggaagac ccaccacatc cagaccggtg tgggccacta cggcgtgttc agcgggcgcc
     6841 gctggaacca gcagatctac ccccgggtac gcgacatgat ccacgccagc gtgggataaa
     6901 cccacgcggc tgcgccatgg tcagcgcatg gcaaaaagtt cctggtggaa gttcagggcg
     6961 ggcaaacctt gcgacaccag gtcttgccgg atggcatcgc caaatgcagt ggggccgcag
     7021 aaccagacgt cggccacacg ccagtccggc acggccgtgc gcaggcgatc cccggtcaaa
     7081 cgtccatcac gggcgtccac caggatgtgc aggcgcacac cggcgttgcg ggcgtcatca
     7141 cgcagcttct gcagcgccac ttcatccacg tccgcagtcg tgtgaaacag gtctacctcg
     7201 cgcccatccg gattcgcggc caatgctttc atgcgcgcga caaacgggga gatgccaatg
     7261 ccgccaccaa tccagatctg gcgcgggctg gtgccctcga agttgaagcg cccatagggc
     7321 ccctccacct ccaccacacc gccaggcttg agggtgctgg tcagcgtacg ggtgtagtcc
     7381 cccagcgcct tgatcaggaa ctgcaactgg ccatcatcct tccagtccga ggaaatcgtg
     7441 tacggatgcg ccccttcgcg ggcatcaaaa ctgacgaaag caaactgccc cgcctggtgt
     7501 cctggccagg cgcttttgag ttgcaccgtc accgcgctga cccccactcc gtccagcgtc
     7561 tccagcgtgc caatcacgcc cacggccttg cgccgactgc caatgcgccg gaacaggctg
     7621 atcacggccg caaccgtgcc acacagcatc aggctggcca tcaccacgcc caagggagtg
     7681 ctccagtagg aaaagcgcat cagcaccacc gagtgcagca ccagcgccag gtacaccacc
     7741 gccatccagc ggtgcgtctt gaagaaatgg cggtacggaa aatacttcac cagggcaatc
     7801 gcaatcagca ccagcgacgc atagaacgcc cattcaccca cgccttcggc cagcccgcgc
     7861 tggccttgca gaaaacccgc aaagtccgcg ctcggtgccg ggcgtgggcc acgcacagga
     7921 cgcgcaatcc agccggcctg caccagccac ttgggtgctt ctacccacaa ccagtgcgca
     7981 atggcgacca ccagaccggt gatgcccagc catttatgca gccggtacat cttgtctagc
     8041 ccatgcaaac ggtgttccag cgccaccggg cgaatggcga gcaccatggc cacactcatc
     8101 acgccgatac cgagtacccc ggtgtactgc aacatctcgt tgcgccaggc aaagacgccc
     8161 tgcatcgcgt accacgccgg ggcatcggcc cacagccaca gcaggctcaa accgagcaga
     8221 aggacgaccc aacttgtttt aatggctttc atggaaacac actccgataa gttgaatcca
     8281 ttgtctgtgg cccaggtaac ccagcggttt gtttcaggtt aagaacgggt aaatctgtct
     8341 cccctttaca caggcgcgca cagtgggtcg taagctgcta tggtgaccca tgatccgcat
     8401 ccagttgaac atcgaactga actacgaaat tgaccagttt ggcgccgatt ttgtgttcaa
     8461 tatccacgct gcccacaccg cgagccagca gatttcgtcc gagaacctgt ttctgagcca
     8521 ggcgatcgat ccgcagatct acaccgaccc ggtcaacggc aaccgctaca tgcgcctgcg
     8581 cgcctggccc ggaccgctca aggtgcaata cagcgccacg gtcgacctga cacaccattt
     8641 ctccaacccg gcgcaggtgc ccgaagtgcc ggtgcgcaac ctgccgcccg aagtcatggg
     8701 ctatatctac cccagccgct actgccagtc ggaccgcctg ctcaaactgg ccaatagcac
     8761 cttcggtggc cagtg
//
LOCUS       DAOSJM010000049         8732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7558_length_8732_cov_5.686182, whole genome shotgun sequence.
ACCESSION   DAOSJM010000049 DAOSJM010000000
VERSION     DAOSJM010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7558_length_8732_cov_5.686182"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PKX82_02905"
     CDS             complement(<1..306)
                     /locus_tag="PKX82_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF51226.1"
                     /translation="MANILVVDDEHGIRDLLWEILNDEGHNVELAENAAQARAARNRE
                     QPDLVLLDIWMPDTDGVTLLKEWSSTGLLSMPVIMMSGHATIDTAVEATKIGALAFLE
                     "
     gene            complement(344..2596)
                     /locus_tag="PKX82_02910"
     CDS             complement(344..2596)
                     /locus_tag="PKX82_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51227.1"
                     /translation="MTHPETGTTPAATVHSSRAVRRSVTVGALVMVVMGLVLLYMLMQ
                     ATNNRELYEQNYARLFIVNVAVASLLLAGIVWVAYRLWLRWRAGRFGSRLLVKLATIF
                     ALVGFAPGLLIYVVSYQFVSRSIETWFDVKVEGALEAGLNLGRVTLDTLSVDLSVKSR
                     AAAAQLTEVPEAMTGIVLERLVDQLGAAEVTLWNSAGSLISSAGQARIQLSPDKPTPA
                     QFRAARVDRTFAWAEGLDDGPANTVGNARIKVLVSISSAALGLTPETRYLLVSRSLPP
                     NLVANALAVESANREYQERALAREGLKRMYIGTLTLSLFLAVFGAILMAVVLGNQIAR
                     PLLLLAEGVSQVAAGDLTPKLAMQGKDELGGLTRAFATMTSQLADAREAVEQSMAQVD
                     AARASLQTILDNLTAGVIVLGTEGQIHSSNPGATRILKVPLAAHEGQALQGIVGLEDF
                     GRAVQAHFSEFLLRRDEHGLDHWQQSFELGAGGSTSHGPFDRALTLVARGAELPDNTR
                     LLVFDDISEIVSAQRAQAWGEVARRLAHEIKNPLTPIQLSAERLEMKLASKLEPTEQA
                     LLVKSVKTIVDQVDAMKRLVNEFRDYARLPAAELKPVDLNALVQDVMQLYESSAGAVT
                     LELDPHCPPVMGDAQQLRQVIHNLLQNAMDASPHGAVVRTQLQSASGRVRLTVLDNGP
                     GFAEHILKRAFEPYVTTKSKGTGLGLAVVKKIADEHGSRVDIANRVVDGAVVGAQVSL
                     SLAVEQSTRA"
     gene            complement(2593..3171)
                     /locus_tag="PKX82_02915"
     CDS             complement(2593..3171)
                     /locus_tag="PKX82_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOF51228.1"
                     /translation="MWCLGALLWLACGLAHAQATADVTQLRVERAEDAIQVSAQLQFE
                     MSPAVEDALLKGIPMVFVAEADVLRERWYWYDKKIATAQRSMRLAYQPLTRRWRLNIH
                     AGSGQGAPVGLALNQSLDTLAQAMAIIKRISQWKIADVGDLDTSLKYRVEFRFRLDMS
                     QLPRPFQIGAIGQSDWDIGCTALVPLSLETGK"
     gene            complement(3182..4507)
                     /gene="rsmB"
                     /locus_tag="PKX82_02920"
     CDS             complement(3182..4507)
                     /gene="rsmB"
                     /locus_tag="PKX82_02920"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOF51229.1"
                     /translation="MEINEGKLPLWRQLQATATVVQAVRGGHSGTAAIETVAGPLRPG
                     VQALAFHVWRNLGRAQALRKQLAPRSPPPAVDALLCTALALAWSDVGAPYDAFTLVNQ
                     TVEAAKRNPATRAQGNFVNACLRRFLRERDSLVALTSADPVAEWNHPQWWIERVQKDH
                     PQHWQQLLQAANAQAPMTLRPNVRQTSRDDYLARLQQGGLAAQADGPTAITLDHAVSV
                     QRLPGFETGAVSVQDGAAQMAAPLLLQGLAGRTSLRILDACAAPGGKTGHLLELTDAA
                     VTALDVDPVRCQRIHDNLSRLRLQASVLTADAAHPAAWWDGQPFDAILLDAPCTASGI
                     VRRHPDVRWLRRETDVAQLASIQRQMLAALWPILKPGGFLLYCTCSVFCAEGRDQVQT
                     FLAHNKDARLMPSPGHLMPRTRTKMDSIPDNETRDHDGFFYALLQKQPA"
     gene            complement(4514..5074)
                     /locus_tag="PKX82_02925"
     CDS             complement(4514..5074)
                     /locus_tag="PKX82_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOF51230.1"
                     /translation="MTGSLLLWIFLALTIFWGVGLYNRLMRIRARGRDALGSVEKHLK
                     GYATVTHAYLMEMGVPEEAIKGVAGEQGLPHGWANLLGTLHALDVACKTARTTPMAIA
                     PMEAVGRGLQNVAHAWAELSSAPADLAGPAVPDTLLKEWEDITQKVHIARGGYNQIVA
                     RYNESIGQFPARLVVGAMGFQPAGTL"
     gene            complement(5161..>8732)
                     /gene="rpoC"
                     /locus_tag="PKX82_02930"
     CDS             complement(5161..>8732)
                     /gene="rpoC"
                     /locus_tag="PKX82_02930"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOF51231.1"
                     /translation="LKVLEAFKKSGIKPEWMVLDVLPVLPPDLRPLVPLDGGRFATSD
                     LNDLYRRVINRNSRLRRLLELKAPEIIARNEKRMLQEAVDSLLDNGRRGKAMTGANKR
                     ALKSLADMIKGKSGRFRQNLLGKRVDYSGRSVITVGPTLKLHQCGLPKLMALELFKPF
                     IFARLEAMGIATTIKAAKKEVESGTPVVWDILEEVIKEHPVMLNRAPTLHRLGIQAFE
                     PILIEGKAIQLHPLVCAAFNADFDGDQMAVHVPLSVEAQMEARTLMLASNNILFPANG
                     EPSIVPSQDVVLGLYYTTRERINGKGEGLIFADTGEVQRAFDAGEVDLNASINVRLTE
                     YTKDKTTGELIPSTKLWETTAGRALLSEILPKGLPFSNLNKALKKKEISKLINVSFRK
                     CGLKETVVFADKLLQAGFRLATKAGISICIDDMLVPEEKHAIIGRAQKEVKEIEQQYV
                     SGLVTSGERYNKVVDIWGKSGDEVSKVMMAKLSKEMVTDRHGKQVQQESFNSIYMMAD
                     SGARGSAAQIRQVAGMRGLMAKPDGSIIETPITANFREGLNVLEYFISTHGARKGLAD
                     TALKTANSGYLTRRLVDVTQDLVVTEQDCGTHSGYLMRAIVEGGEVIESLRDRILGRT
                     AADDVLHPENRSVLAPAGTMLDEDLIEELEAAGVDEVKVRTALTCETRFGICAKCYGR
                     DLGRGGLINHGEAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAIASSVEAKSNGSIG
                     FNATMRYVTNSKKELVVISRSGEIVIHDEHGRERERHKVPYGAVLTVKADQVVKAGAI
                     LANWDPLTRPIITEFAGKAHFENVEEGLTVAKQVDEVTGLSTLVVIDPKRRGAAKVVR
                     PQVKLIDASGHEVKIPGTDHSVTIGFPVGALVQVRDGQDVGPGEVLARIPIEGQKTRD
                     ITGGLPRVAELFEARSPKDKGVLAEVTGTVSFGKETKGKVRLQITDPEGKVWEELVPK
                     EKNILVHEGQVVNKGESVVDGPADPQDILRLLGSEELARYIVDEVQDVYRLQGVKIND
                     KHIEVIVRQMLRRVVVEAAGDSNYINGEQVERSEMLNTNDALRAEGKIPAVFTNLLLG
                     ITKASLSTDSFISAASFQETTRVLTEAAIMGKRDELRGLKENVIVGRLIPAGTGMAYH
                     EARKVREKMDDAERRAIAEAEALELAGGADAVEAQANEEGTGE"
BASE COUNT         1681 a         2744 c         2573 g         1734 t
ORIGIN      
        1 ttccaggaag gccagggcac cgatcttggt agcctcgaca gcggtatcaa tggtggcgtg
       61 gccgctcatc atgatgacgg gcatggacaa caggccagtg gaagaccatt ccttgagcag
      121 cgtgacacca tcggtatcgg gcatccagat gtccagcaga accaggtcag gctgttcgcg
      181 gttgcgcgct gcgcgcgcct gggccgcatt ctcggcaagc tctacgttgt gaccctcgtc
      241 attgagaatt tcccagagca ggtcccgaat gccgtgctcg tcgtctacaa ccagaatatt
      301 tgccatggta atcgcgcagt ccctgtggtc ccatatgccg cgctcaagcg cgcgtgcttt
      361 gctctacggc caatgataac gatacttgcg cccctactac cgcaccgtca acgacgcgat
      421 tggcgatgtc gacgcgcgag ccatgttcgt cggcaatttt cttgacgacc gccagcccca
      481 atcctgttcc cttggacttg gtcgtgacat agggttcgaa tgcccgcttc aggatgtgct
      541 ccgcaaatcc ggggccgttg tcgagcacag tcagacgtac gcgtccggac gccgattgca
      601 gctgcgtgcg caccaccgca ccatggggac tggcatccat ggcattttgc agcaggttgt
      661 ggatgacctg gcgaagctgc tgtgcatctc ccatgacagg cggacagtgg gggtcaagct
      721 ccagcgtcac tgcgccagcg gacgactcat acaactgcat gacgtcctgc accagcgcat
      781 tgaggtctac cggtttgagc tcagccgcag gcaagcgggc gtagtcacgg aattcattga
      841 ccagccgctt catggcatcc acctgatcca cgatggtttt gacggacttg accagcaacg
      901 cctgttcggt cggttcgagt ttggacgcca gcttcatctc cagccgctcg gcggacagtt
      961 gaatgggggt gagtgggttc ttgatctcat gcgccagccg gcgggccacc tcgccccagg
     1021 cctgcgcccg ctgcgccgag acgatctcgg aaatatcgtc gaacaccagc agtcgtgtgt
     1081 tgtccggcag ctccgccccg cgtgccacca gggtcagcgc gcgatcaaac ggtccgtgcg
     1141 atgttgagcc gcccgcaccc agctcgaagg actgctgcca gtgatccaga ccatgctcgt
     1201 cgcggcgcag gaggaactcg gagaaatgcg cctgtactgc ccggccaaaa tcctccagtc
     1261 ccacaatgcc ctgcaaggcc tgtccctcgt gcgcagccag cggcaccttg agaatccggg
     1321 tggcacccgg attggacgag tgtatttgcc cctctgtacc caacacgatg acacctgcag
     1381 tcaggttatc cagaatcgtc tgcaagctgg cgcgcgccgc atcgacctgg gccatgctct
     1441 gctccacggc ttcgcgcgca tccgccagct gcgatgtcat ggtggcaaag gcacgcgtca
     1501 aaccacccag ctcgtccttg ccctgcatgg ccagcttggg ggtcaagtct cctgcagcca
     1561 cctgactgac accttcggca agcagcagca agggccgcgc gatctggttg cccagaacca
     1621 ccgccatgag aatggcaccg aacaccgcaa ggaacagact cagggtcagt gtgccgatgt
     1681 acatgcgttt gaggccttcg cgcgccaacg cccgctcctg gtattcccgg ttcgcgctct
     1741 ccaccgccag cgcattggca accaggttcg gcgggaggct gcgggaaacc agcaggtagc
     1801 gggtttccgg agtcaaaccc agtgccgcac tggaaatgga caccagcacc ttgatgcgcg
     1861 cgtttcccac cgtattggcc gggccgtcgt ccagaccctc tgcccacgca aatgtgcggt
     1921 ctacccgggc agcccggaac tgggccggtg tgggtttgtc cgggctgagc tggatgcgcg
     1981 cctgtccggc gctggagatc agacttccgg cgctgttcca gagagtcact tccgctgcac
     2041 cgagctggtc caccagtcgc tccagcacga tgccggtcat ggcttctggc acctcggtga
     2101 gctgtgctgc ggctgcccgt gatttgaccg agaggtcgac cgacagggtg tcgagggtaa
     2161 ccctccccag attgagaccc gcctccagtg cgccttcgac cttcacatca aaccaggttt
     2221 caatggagcg ggaaacgaac tggtacgaca caacataaat cagtagccct ggtgcaaaac
     2281 ccaccagcgc aaagatggta gccagcttga ccagcaggcg actgccaaac cggcccgcgc
     2341 gccaccgtag ccagagccgg taagccaccc agacgatgcc cgcaagcagc agcgatgcca
     2401 ccgcgacatt gacaatgaac aggcgcgcat agttctgctc gtagagttca cggttgttgg
     2461 ttgcctgcat cagcatgtag agcaggacca gacccatcac gaccatgacg agggcaccca
     2521 cggtcactga ccgccgcacg gcgcgagagc tatggaccgt ggccgctggc gtggtgccag
     2581 tttccggatg ggtcatttac cagtctctag ggagagcgga acaagggcgg tacaaccgat
     2641 gtcccagtcc gactgcccga tggcgccaat ctgaaaaggc cgtggcaact ggctcatgtc
     2701 cagccggaag cggaattcca cccggtactt caaggaggtg tcgagatcgc cgacgtcggc
     2761 aattttccat tgggatatgc gcttgatgat ggccatggcc tgggccagcg tatcgagact
     2821 ctggttcagc gccaacccca ccggggcgcc ctgcccggag cctgcatgga tattgaggcg
     2881 ccaacgccgc gtaaggggct ggtacgccag acgcatgctg cgctgtgccg tcgcaatttt
     2941 cttgtcgtac cagtaccagc gctccctcag tacgtcggcc tcggccacaa agaccatcgg
     3001 gatgcctttg agcaaggcat cctccactgc tggcgacatt tcaaactgca gctgggccga
     3061 tacctggatc gcatcttcag cgcgttcgac gcgcaattgg gttacgtcag ctgtagcctg
     3121 cgcgtgtgcc agtccgcacg cgagccacag cagtgcccca aggcaccaca aaacagcctg
     3181 ctcaagcggg ctgcttctgc aatagcgcgt aaaagaatcc atcgtggtca cgggtttcat
     3241 tgtccgggat ggagtccatt ttggtgcggg ttcgcggcat taaatgcccg ggggaaggca
     3301 tcaggcgcgc gtctttgttg tgtgcaagaa acgtttgtac ctggtcacgc ccttctgcac
     3361 aaaaaacgga gcatgtgcaa tacagaagga atccgcctgg cttcaggatc ggccagaggg
     3421 cggcgagcat ctgtctttgt atggaggcca attgggccac atcggtttcc ctgcgcaacc
     3481 agcgcacatc cggatggcgc cgcacgatgc cggatgcggt acagggcgcg tccagcagga
     3541 tggcatcgaa cggctgccca tcccaccatg ctgccggatg ggccgcatcg gcggtcaata
     3601 cggaagcttg caggcggagc cgcgacaggt tgtcgtgaat acgctggcat cgcacgggat
     3661 cgacgtccag tgcggtgact gctgcgtccg tgagttcgag caggtgcccg gtttttccac
     3721 caggggcggc gcaggcgtcg agtatgcgca acgaagtccg cccggccagt ccttgcagca
     3781 gcaagggggc cgccatttgc gcagcaccat cctgcaccga taccgcgcca gtttcaaagc
     3841 cgggcagtcg ctgcaccgat accgcgtggt ccagtgtgat ggccgtaggt ccatctgcct
     3901 gtgccgccag tccgccttgc tgcaggcgcg cgagatagtc gtccctgctt gtttgccgca
     3961 cattggggcg cagggtcatg ggtgcctggg cattggctgc ctgcaacaac tgctgccaat
     4021 gctgcggatg gtccttctgg acacgctcaa tccaccattg cgggtggttc cactcggcca
     4081 cgggatccgc gctggtcagc gcgaccaggc tgtcgcgttc gcgcagaaaa cggcgcaggc
     4141 acgcattgac aaagtttccc tgcgcgcggg tggccgggtt gcgtttggct gcctctaccg
     4201 tctggttgac cagggtaaag gcgtcgtagg gtgcgcctac atctgaccac gccagcgcca
     4261 gcgccgtgca cagcagtgcg tccaccgcgg gaggtggcga cctgggcgcc agctgcttgc
     4321 gcagtgcctg ggcacgcccc agattgcgcc agacatgaaa ggccagcgcc tggacgccag
     4381 gacgcagggg gccggcaacc gtttcgatcg cggccgtccc ggaatggccc cctcgcacgg
     4441 cctgcaccac cgtcgccgtg gcttgcaact ggcgccacag cggcagcttt ccttcattga
     4501 tttccatgtt gacctataag gttcctgccg gttgaaagcc catcgctccg accaccaggc
     4561 gcgccgggaa ctgcccaatg gattcgttgt accgcgccac gatctggttg tagcctccgc
     4621 gcgcgatgtg aaccttctgg gtaatgtctt cccattcttt gagcagcgtg tcaggtacgg
     4681 cggggcctgc caggtctgcg ggcgcgctgg atagttcggc ccaggcatgg gccacgttct
     4741 gcaggccccg gcccacggct tccatgggag cgatggccat cggagtcgtg cgggcggtct
     4801 tgcacgccac gtccagcgca tgcaaggttc ccagcaagtt cgcccatcca tggggcaacc
     4861 cttgttcgcc tgccacccct ttgatcgcct cctccggcac acccatttcc atgagatagg
     4921 catgcgttac ggtggcatag cctttgagat gcttctcgac ggacccgaga gcatcccgtc
     4981 cacgcgcgcg gatgcgcatc aagcggttgt acaagcccac accccagaaa atggtgaggg
     5041 caagaaaaat ccacagcagc agtgatccag tcatgtgtga tttgccttac acaacaaaaa
     5101 cgccccaagc cccccgtaac gagagcttgg ggcgctacca atagagtagc agaacgagtt
     5161 ttactcgcca gtaccctctt cgttggcctg tgcttctact gcatctgcac cgccagccag
     5221 ctccagagcc tcggcttcag cgatggcacg gcgctcggca tcgtccatct tttcacggac
     5281 cttgcgggct tcgtggtacg ccataccggt accggcagga atcaggcgac ccacgatcac
     5341 gttttccttc aagccacgca actcgtcgcg cttgcccatg atggcagctt cggtcagcac
     5401 gcgggtggtt tcctggaagg acgccgcgga gatgaacgag tcggtcgaca gggatgcctt
     5461 ggtaataccc aacagcaggt tggtaaagac cgcagggatc ttgccttcgg cacgcaatgc
     5521 atcgttggta ttgagcatct cggaacgctc aacctgttca ccattgatgt aattggaatc
     5581 acccgcagct tccaccacca cacggcgcag catctggcga acaatcactt caatgtgctt
     5641 gtcgttgatc ttcacgccct gcaagcggta cacgtcctgg acttcgtcca cgatgtaacg
     5701 tgccagttct tcggagccga gcaaacgcag aatgtcttgc ggatcggcag ggccgtccac
     5761 cacggactcg cccttgttga cgacctggcc ttcgtgcacc aggatgttct tttccttcgg
     5821 aaccagctct tcccagacct tgccttcggg gtcagtgatc tgcaggcgaa ccttgccctt
     5881 ggtttccttg ccgaacgaca ccgtaccggt gacttcggcg agaacgcctt tgtctttcgg
     5941 cgaacgggct tcgaacagtt cggccacgcg gggcagaccg ccggtaatgt cgcgggtctt
     6001 ctgaccttcg attgggatac gggccagcac ttcgccagga cccacgtcct gaccgtcacg
     6061 cacctgcacc agcgcaccca cggggaagcc gatggtcacc gagtgatcgg tacccgggat
     6121 cttcacttcg tggccggagg cgtcgatcag tttgacctgg gggcgcacga ccttggcagc
     6181 accgcggcgc ttgggatcga tcaccaccag ggtggacaga ccggtcactt cgtcgacctg
     6241 cttggccacc gtcagacctt cttccacgtt ctcgaaatgc gccttaccag cgaactcggt
     6301 aatgatgggg cgtgtcaacg gatcccagtt ggccagaatg gcgccggcct tgaccacctg
     6361 gtctgccttg accgtcagca ccgcgccgta gggcactttg tgacgctcac gctcacgtcc
     6421 gtgctcgtcg tggatcacga tttcgccgga acgggaaatg acaaccagtt ccttcttgct
     6481 gttggtcacg taacgcatgg tggcgttgaa accaatcgaa ccattggact tggcttccac
     6541 gctggaggca atggcagcac gcgatgccgc accaccgatg tggaaggtac gcatggttag
     6601 ctgggtaccg ggttcgccga tggactgcgc agcgatcaca ccgacggctt cgccgtggtt
     6661 gatcagacca ccacgtccca gatcgcgtcc gtagcacttg gcacagatac cgaaacgtgt
     6721 ttcgcatgtc agtgcggtgc gcaccttgac ttcgtccacg ccagcggctt ccagctcctc
     6781 gatcaggtct tcgtccagca tggtaccggc gggagccagc acagagcggt tttccgggtg
     6841 cagcacgtca tccgcagccg tacggcccag gatgcggtca cgcagggact cgatcacttc
     6901 accgccttca acgatggcgc gcatcaggta accactgtgg gttccgcaat cctgctctgt
     6961 caccaccaga tcctgggtta cgtccaccag acgacgggtc agataacccg agttcgccgt
     7021 cttcaaagcc gtgtcggcca gacccttacg ggcaccgtgg gtggagatga agtactccag
     7081 cacgttcaga ccttcgcgga agttcgcagt aataggcgtc tcgatgatgg agccgtctgg
     7141 cttggccatc agaccccgca tacccgccac ctgacgaatc tgcgctgcag aaccgcgggc
     7201 accggagtcg gccatcatgt aaatggaatt gaaggactct tgctggacct gcttgccatg
     7261 gcggtcggtg accatttcct tggacagctt ggccatcatc accttggaca cttcgtcacc
     7321 cgacttgccc cagatgtcca ccaccttgtt gtagcgctcg ccggaagtca cgagacccga
     7381 gacatattgc tgctcgattt ccttgacttc cttttgtgcg cgaccaatga tggcgtgctt
     7441 ctcttcaggc accagcatgt cgtcgataca gatggagata ccggccttgg tcgcgagacg
     7501 gaagcccgct tgcaacagct tgtcggcaaa caccacggtc tctttcaatc cgcacttgcg
     7561 gaaagacacg ttgatcagct tggagatttc cttcttcttc agggccttgt tcaggttgga
     7621 gaagggcagt cccttgggca gaatctcgga cagcaacgca cgcccggcag tggtttccca
     7681 cagcttggtg gacgggatca actcgccggt ggtcttgtcc ttggtgtact cggtcagacg
     7741 cacattgatg ctggcattca gatccacttc gcccgcgtca aatgcacgct gcacttcacc
     7801 ggtgtcagcg aagatcaggc cttcaccttt gccgttgata cgctcgcggg tcgtgtagta
     7861 cagacccagc accacgtcct gggacggaac gatggaaggc tcgccattgg cagggaacag
     7921 gatgttattc gaagccagca tcaaggtgcg ggcttccatc tgcgcttcca cagacagggg
     7981 cacgtgcaca gccatctggt caccgtcgaa gtcggcgttg aaggccgcgc aaacgagggg
     8041 gtgcaactga atggccttgc cttcgatcag gataggctcg aaggcctgga tacccaaacg
     8101 gtgcagcgta ggcgcacggt tcagcatcac ggggtgctct ttaatgacct cttccaggat
     8161 gtcccaaacc acgggggtgc cggattcaac ttccttcttg gccgccttga tggtggtggc
     8221 aatgcccatg gcttccaggc gcgcgaagat gaaaggcttg aacagttcca gagccatcag
     8281 cttgggcaga ccgcactggt gcaacttgag tgttggaccc acagtaatca cggaacgacc
     8341 agagtagtcc acgcgcttgc ccagcaagtt ctggcggaaa cgaccggatt tacccttgat
     8401 catgtcagcc aaagacttca gcgcacgctt gttggcgccg gtcatggcct tgccgcgacg
     8461 gccgttgtcc agcaggctgt ccacggcttc ttgcaacatg cgcttttcgt tgcgcgcaat
     8521 gatttccggt gccttcagct ccagcaaacg gcgcagacgg ctgttgcggt tgatcacgcg
     8581 gcggtacagg tcgttcaggt cggaagtcgc aaagcggccg ccatccagcg gaaccagcgg
     8641 acgcaggtcc ggtggcagca cgggcaacac gtccaacacc atccactcag gcttgatgcc
     8701 ggacttcttg aacgcttcca ggaccttcaa ac
//
LOCUS       DAOSJM010000050         8708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7581_length_8708_cov_4.727493, whole genome shotgun sequence.
ACCESSION   DAOSJM010000050 DAOSJM010000000
VERSION     DAOSJM010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7581_length_8708_cov_4.727493"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PKX82_02935"
     CDS             complement(<1..238)
                     /locus_tag="PKX82_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOF51232.1"
                     /translation="MFARLRSDIQCILDRDPAARSTWEVLTCYPGLHAVVLHRLAHWC
                     WTHGLQWPGRFISHIARFFTGIEIHPGAKIGQRVF"
     gene            complement(248..1153)
                     /locus_tag="PKX82_02940"
     CDS             complement(248..1153)
                     /locus_tag="PKX82_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOF51233.1"
                     /translation="MQTRFILINTSHAGNVGAAARAMKTMGFDDLVLVAPRWANVLRR
                     EETIQRASGALDVLEKCRIVETLDEALDGMTHLCATAMTPRDFGPPTTTPREHFEMLS
                     KKEHCAHIPRGLEPDLASKTGAEGQFSAGPPQGALDRLAASAARQLAPSGGSDPRSGG
                     AWGPQSGVGFLFGSERFGMRNEDVYRCHVCLSIPSNPKFGSLNIGAALQVIAYEWRLA
                     LGGFSQQAATAPSTLADAGQVAGMLGHLEQSLVHLGFLDPAAPKKLMPRLNALFNRAD
                     VTQEEIHILRGIAKAILQTEPPRRG"
     gene            1276..>2157
                     /locus_tag="PKX82_02945"
     CDS             1276..>2157
                     /locus_tag="PKX82_02945"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOF51234.1"
                     /translation="MSPNLHPMINVAVKAARAAGSIINRAALDIESVRISQKKANDFV
                     TEVDHAAEQAIIETLLTAYPGHGIWAEESGREHGAKDSEFVWIIDPLDGTTNFIHGLP
                     VYCVSIALSVKGKIEQAVVYDPTRNDLFTATKGRGAYLNDRRIRVSKRTELKQSLIST
                     GFPFRPGDNFNQYLAMMGDVMQRTAGLRRPGAAALDLAYVAAGFTDGFFEFGLQPWDV
                     AAGSLLITEAGGLVGNFTGEADFLEQQECLAGTPRVYGQLVSLLGKYSKFASAGEKAS
                     LRQAAQAPAPAEESSGSS"
     assembly_gap    2158..2257
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2346..5258)
                     /locus_tag="PKX82_02950"
     CDS             complement(2346..5258)
                     /locus_tag="PKX82_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF51235.1"
                     /translation="MGITESADSAPMQADMLIGTFDESPMAISVSRLSDGCFVHVNAR
                     WCELMHMTREEVLGKDSVFVGVWADADARRKVLSQMRLAGKAPDGVPPSVTFPYRRRD
                     GREMQLQMQGARVNLAGVDHVITYVTDVTDQHRARQALQHNQNVLERLNEDISSQMEL
                     YVLTESLARVGHWKVDAGDSRPQWSQGLYDLTGLENTGSLSFTEARSRIAPQDWPLFM
                     AARERLDGEMVEYRWNHPDGEVRWLRSRMRRHHRADGSFVDIGVVQDYTSERKTKMAL
                     QDQLDVIQRLTSKLPVMVFQLARLDETHNRFVFVNDAVETIFGVTPEQAVADAKAVFR
                     RVHHDDIGGVVKTMQDSARDRTPWVHEFRVQQPDGAVRFLLGKAMVAEEHSGQVVAYG
                     SVTDLTDQRASQVSLMESESRFRALTELSSDWYWEQDAQFRFVRLDGSLVNQAGRTGN
                     SSLGKTRWEVGALNMTAQDWEAHRSVLEAHAEFRDLELRDVDYRGRPFWMSISGAPFF
                     DAQGQFQGYRGVGRNITARKRAEEKITRLAFFDVLTGLPNRRLLMDRLQQALAVSARD
                     RSAGALLFIDLDNFKDLNDSQGHDVGDLLLKEVAARIETCLREVDTVARLGGDEFIVM
                     LQSLGPDLAQAAAQAEVVAQKILAALNQTYMLRTLEHHSTPSIGITLFEDHIQTVDEL
                     LKQADLAMYESKAAGRNTLRFFDPEMQAMVAQRTELDSDLRLGLRRGELVLYYQPVVD
                     AHRRVTGVEALVRWRHPAKGLVPPADFIPMAEQSGLILPLGDWVMETACLQLAQWARL
                     GATSNLTMAVNVSARQFRRPDFVQRVEALLETTGAKAPLLKLELTESLLLSDVQDAVR
                     KMTELGARGVKFSLDDFGTGYSSLTYLKMLPLEQLKIDQSFVRDVLTDANDAAIARTV
                     LALGHSLGLSVVAEGVETAGQLEFLLQHGCTQFQGYLFGRPVPIEALQLDDPAAYI"
     gene            complement(5261..7831)
                     /locus_tag="PKX82_02955"
     CDS             complement(5261..7831)
                     /locus_tag="PKX82_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF51236.1"
                     /translation="MKPARSVEKTPDNTTSGGTAPLGDGRLDFFGVDVGAMFKAFFDQ
                     SPEAIALTRVRDDAIVEVNQEWVKLTGLPRDQVLGRTAVEIGHWRDAGMRAQAMHGIK
                     TNGRVTDLDVMLNMAGGVARLVRLNAVLVHVPAGPFVLIYLRDVTAELMAQEALKATE
                     LELAQTNEKLNRQVKLHEMTEAMARLGHWVHYPGAEEVQLSPGYSVLANIGDTTHAPV
                     GDHLKSVVPEDRDIVTGALKRMDGKLVEYRWHRPDGRVVWIRSRMHRQIENGVVKADF
                     GIVQDITAERESMQQVAEQLAAMRQSEARFRALTELSSDWFWEQDEQFRFTRVDGNLE
                     ASNALPSPSYVGKTRWDSGVQGVSPERWAAHRAAVEAHEVFHDFEMQRLRADGSPMWV
                     TISGAPIFDDNEVFKGYRGTGRDISERKRAEADILRLAFYDSLTGLPNRRLLMDRLTQ
                     AMEFSARRISHGALLFIDLDNFKVLNDTLGHQMGDELLKQVAERLIQCVRSVDTVARL
                     GGDEFVVMMEDMGESPIDAAAQAEMVGKKILGALNQEFLLAGNRHHSSPSIGITLFFQ
                     HMHTLDELLKRADLAMYQAKGAGRNTMRFYDPEMQAVASARAVLEADMRLGLQRSEFM
                     LHYQPVVDEQATMTGVEALLRWNHPKRGLISPAEFIPVAEQTGLILQLGAWVLEQACA
                     QLVAWSAVPSTQRLSIAVNVSARQFRHSDFATQILSLLRTSGANPYRLKMELTESLLL
                     SDIDDAIDKMGELRSIGVGFSLDDFGTGYSSLSYLKRLPLDQLKIDQSFVRDVLTDPN
                     DAAIARTILNLAQSLDLGVVAEGVETEGQRDFLLKNGCRAFQGYLFGRPVPLHQLELS
                     "
     gene            7967..>8708
                     /locus_tag="PKX82_02960"
     CDS             7967..>8708
                     /locus_tag="PKX82_02960"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOF51237.1"
                     /translation="MKTKITELFGIEHPIIQGGMHFVGFAELASAVSNAGGLGMITGL
                     TQRSPKDLANEIRRCREMTDKPFGVNLTFLPTVSSPDYPGYIQAIIDGGVKAVETAGN
                     NPQKYLPALHEAGIKVIHKCTSVRHALKAESIGCDAVSVDGFECGGHPGEDDVPNFIL
                     LPRAADELKIPFVASGGMADGRSLAAALCLGAEGINMGTRFIATQEAPVHANVKQAIV
                     AATELDTRLVMRPLRNTERVLRNAAVNRL"
BASE COUNT         1694 a         2846 c         2441 g         1627 t
ORIGIN      
        1 agaacacgcg ctggccgatc ttggcgccgg gatgaatttc aatccccgtg aaaaagcggg
       61 cgatatggga aataaaccgc ccgggccatt ggagtccgtg cgtccagcac cagtgcgcca
      121 gacggtgcag gaccactgca tgcagaccgg gatagcaggt cagcacctcc caggtgctgc
      181 gcgcagccgg gtcgcggtca agaatgcact ggatatcgga gcgtaggcgg gcaaacatgc
      241 gtctagtcta tcccctgcgc ggaggctctg tctgcaggat ggctttggca ataccgcgca
      301 aaatatggat ttcctcttgg gtgacatccg cccggttgaa cagtgcattg agcctgggca
      361 tcagtttctt gggcgccgca gggtccagaa aacccagatg caccagcgac tgctccagat
      421 gccccagcat gccagcgacc tgccccgcat cggccagggt gctgggcgcg gtggcggctt
      481 gctggctgaa cccgcccagc gccagacgcc attcgtaggc aatcacctgc agcgccgcac
      541 caatattcag cgagccgaat ttcgggttgc tgggaatact caggcagaca tggcagcggt
      601 acacatcttc gttgcgcatg ccgaaacgct cggaaccgaa caaaaacccc acaccagact
      661 gtggccccca cgctccaccg ctacgcgggt cgctgccccc cgagggggct aattgcctag
      721 cggcgctggc cgccaagcgg tccagagctc cttggggcgg cccggcggag aattgcccct
      781 cagcccctgt ctttgatgcc aaatcgggct ctagcccccg aggaatatgc gcacaatgct
      841 ccttttttga tagcatttca aaatgctcac gcggcgtggt cgtgggcggg ccaaagtcgc
      901 gcggcgtcat ggcggtggcg cacaggtggg tcatgccatc cagcgcctcg tccagcgttt
      961 ccacaatgcg gcatttctcc agcacatcca gggctccact ggcccgctgg atggtttctt
     1021 cgcggcgcag cacattggcc cagcgcggcg ccaccagcac cagatcgtca aaacccatgg
     1081 tcttcatggc ccgggcggca gcgcccacat tgccggcatg gctggtgttg ataaggatga
     1141 atcgggtctg catgcggatg agctcaggga aaaatgggca ttagggtgtg caaccgagcc
     1201 cctgccgtta aaatagccct attgtcgccg cctgcatcgc cagccggtcc cgttttgctc
     1261 ttcccccaac aatctatgtc gcccaatctg caccccatga tcaacgtggc cgtcaaggcc
     1321 gcccgcgccg ccggttccat catcaaccgc gctgcgctgg atatcgagtc cgtccgcatc
     1381 tcccaaaaga aagccaacga ctttgtcacc gaagtggacc atgctgcaga gcaggcgatc
     1441 atcgaaaccc tgctgacggc ctatcccggt cacggcatct gggcagaaga atccggccgt
     1501 gaacacggcg ccaaagactc cgaattcgtc tggatcatcg atccgctgga cggcaccacc
     1561 aacttcatcc acggcctgcc ggtgtactgc gtcagcattg cgctgtccgt caagggcaag
     1621 atcgagcagg cggtggtgta cgaccccacc cgcaacgacc tgttcaccgc caccaagggc
     1681 cgcggtgcct acctgaacga ccgccgcatc cgcgtctcca agcgcaccga actcaagcag
     1741 agcctgattt ccaccggctt cccgttccgc ccgggtgaca acttcaacca gtacctggcc
     1801 atgatgggcg atgtgatgca gcgcaccgcc ggcctgcgcc gccctggtgc cgccgcgctg
     1861 gacctggcct atgtggctgc cggtttcacc gatggtttct tcgaatttgg cctgcagccc
     1921 tgggatgtgg ccgcaggatc gctgctgatc accgaggcgg gcggcctggt gggtaacttc
     1981 acgggcgaag ccgatttcct ggaacaacag gaatgcctgg ccggcacccc gcgtgtgtac
     2041 ggacaactgg tgagcctgct gggcaagtac agcaagtttg ccagcgccgg agaaaaagcg
     2101 tccctgcgcc aggcggcgca agctcctgca ccggcagaag aaagcagcgg cagcagcnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct gcgccaggcg gcgcaagctc
     2281 ctgcaccggc agaagaaagc agcggcagca gcgacgcgcc gttttaagcg ctcgttgcgg
     2341 ggcggtcaga tataggccgc agggtcgtcc agttgcagtg cctcaatcgg caccggacga
     2401 ccaaacaggt agccctgaaa ctgcgtgcat ccgtgctgca acagaaactc cagttgccct
     2461 gcggtttcca cgccttctgc cacgacactg agcccgagac tgtggcccag tgccagcacg
     2521 gttctggcaa tggcagcgtc gttggcatcg gtcagaacat cgcggacaaa cgactggtcg
     2581 atcttgagct gctccagtgg cagcatcttc aggtaggtga gcgacgaata cccggtcccg
     2641 aagtcgtcca gcgagaattt gacaccccgt gcgcccaact cggtcatctt gcgcaccgca
     2701 tcctgcacat cgctcagtag cagactctcg gtcagctcca acttgagcaa cggggctttg
     2761 gcaccggtcg tctccagcag ggcttccacc cgctgcacaa aatcagggcg ccggaactgg
     2821 cgtgcactga cattcacggc catcgtcagg ttcgacgttg cgccgagtcg tgcccattgc
     2881 gccagctgca gacatgcggt ttccatgacc cagtcgccca gcggcagaat cagaccactc
     2941 tgctccgcca tgggaataaa gtccgccgga ggcaccagcc ccttggcagg gtgccgccag
     3001 cgcaccagcg cttccacccc ggtgacgcgt cggtgcgcat ccaccacggg ctggtaatac
     3061 agcaccagtt ctccgcgccg caggcccagc cgcaaatcac tgtcgagctc cgtccgctgg
     3121 gccaccatgg cctgcatctc gggatcgaaa aagcgcaggg tattgcgccc tgcagccttg
     3181 gactcgtaca tggccagatc agcctgcttg agcaactcgt ccacggtctg gatgtggtct
     3241 tcaaacagcg tgataccaat gctgggggtg ctgtggtgct ccagggtgcg caacatgtag
     3301 gtctggttga gggcagccag gattttctgc gccacgactt ccgcctgggc tgccgcctgg
     3361 gccaggtcag gccccagtga ctggagcatc acgatgaact cgtcgccacc caggcgtgcc
     3421 acggtatcca cttcgcgcag gcaggtttca atgcgcgcgg cgacctcttt caacagcaga
     3481 tcgcccacgt catgcccctg ggagtcgttc aggtccttga agttatccag gtcaatgaac
     3541 agcaaggctc cggcggaacg gtcccgtgca ctcacggcca gcgcctgctg caagcggtcc
     3601 atcagcaggc gacggttggg cagacctgtc aacacatcaa aaaatgccag ccgcgtgatt
     3661 ttttcctcag cacgtttacg cgccgtgatg ttgcgcccca cgccccggta gccctgaaac
     3721 tgtccctgtg catcgaagaa gggggctccg ctgatcgaca tccagaaagg acgtccccgg
     3781 taatccacgt cgcggagctc cagatcccgg aattcggcat gggcttccag caccgaccgg
     3841 tgcgcctccc agtcttgcgc agtcatgttg agcgcaccca cttcccaacg cgtcttgccc
     3901 aggctgctgt ttccggtgcg tccggcctgg ttgaccaggc tgccatccag ccgcacaaag
     3961 cggaactgag catcctgctc ccagtaccag tcggaagaca actcagtgag cgcacgaaag
     4021 cgcgattcac tctccatcag cgagacctgg gacgcacgct gatccgtcag gtcggtcacg
     4081 gaaccgtacg ccaccacctg ccccgaatgc tcttccgcga ccatggcttt gccgagaaga
     4141 aaccgcaccg ctccatcggg ctgctgaacg cgaaactcgt ggacccaggg cgtccggtcc
     4201 ctggcgctgt cttgcatggt cttcaccacg ccgccgatat cgtcatggtg aacccgccgg
     4261 aacacggctt tcgcatcggc aaccgcctgc tccggcgtca cgccaaaaat ggtttccacc
     4321 gcatcgttga caaatacgaa tctgttgtgc gtttcgtcga gcctcgcaag ttgaaagacc
     4381 atcacgggca gcttgctggt caaccgctgg atcacgtcca gttggtcctg cagcgccatc
     4441 ttggtcttgc gctcgctggt gtagtcctgc accacaccaa tatccacaaa actgccatcc
     4501 gcccggtgat gcctgcgcat gcggctgcgt aaccagcgga cttcgccgtc ggggtggttc
     4561 cagcggtatt cgaccatttc cccatccaga cgctcacgtg cggccataaa caggggccag
     4621 tcctgcggcg caatgcgcga cctggcttct gtgaaggaca gacttcccgt gttttcgagg
     4681 cctgtcaggt cgtacagtcc ctgggaccat tggggccgag aatcgccggc atcgaccttc
     4741 cagtgcccca ccctggccag actttcggtc aacacgtaca gctccatctg actggagata
     4801 tcttcgttga gccgctcaag tacgttctgg ttgtgctgca aagcctgccg cgcccggtgt
     4861 tggtccgtca cgtccgtgac gtaggtaatc acatggtcta cgcccgccag attgactctt
     4921 gcaccctgca tctggagttg catttcgcga ccatcacgcc gccgatatgg aaatgtcacg
     4981 gatggtggaa caccgtctgg cgccttgccg gcaagcctca tctggctcaa aaccttgcga
     5041 cgcgcgtcgg catccgccca tacgccgaca aacaccgagt ccttccccag cacctcttca
     5101 cgcgtcatgt gcatgagctc acaccagcgc gcgttcacat ggacaaagca cccgtcgctc
     5161 aggcggctca ccgagatcgc catcgggctc tcgtcaaaag tgccgatcag catgtcagcc
     5221 tgcatagggg cgctgtcggc cgactctgta attcccatgc ctaggacaac tccagctggt
     5281 gcaaaggtac gggccgccca aacaggtatc cctggaacgc cctgcagcca ttcttcagca
     5341 ggaaatcacg ttgcccctcg gtttccaccc cttcagccac cacacccaga tccagacttt
     5401 gggccagatt cagaatggtg cgcgcaatgg cggcgtcatt gggatcggtc aacacatcac
     5461 gtacaaagga ttggtcgatc ttgagttggt ccagcggcag gcgtttgagg tagctcaagg
     5521 aagaatatcc ggtaccaaaa tcgtccagcg agaagcccac gccgatggag cgcaactcgc
     5581 ccatcttgtc aatggcatcg tcgatatcgc tgagcaacag gctctccgtc aactccatct
     5641 tgaggcgata gggattggct ccgctggtgc gcaaaaggga aagtatctgt gtcgcaaaat
     5701 cagaatgccg gaactgccgc gcactgacat tgacggcaat agacagtcgc tgggttgaag
     5761 gcaccgcgct ccaggccacc agctgcgcgc aagcctgctc cagcacccat gccccaagtt
     5821 gcagaatcag accggtctgc tccgccactg gaatgaactc tgccggagaa atcaggcccc
     5881 gtttgggatg gttccaacgc agcagggcct ccacgccggt catggtcgcc tgctcgtcca
     5941 ccaccggctg gtaatgcagc atgaactcgc tacgctgcag tcccaggcgc atgtctgctt
     6001 ccagcactgc ccgcgcactg gcgaccgcct gcatctcggg gtcataaaaa cgcatcgtgt
     6061 tgcgcccggc gcctttggcc tggtacatgg ccagatcggc gcgtttgagc agctcatcca
     6121 gcgtatgcat gtgctggaaa aacagggtga tgccaatgct cggtgagctg tggtggcggt
     6181 ttccggcgag taggaactcc tggttcaacg caccgagaat cttcttgccc accatttccg
     6241 cctgggctgc cgcgtcgatg ggcgactcgc ccatgtcctc catcatcacc acgaactcat
     6301 cgccccccag gcgcgccact gtatccacgc tgcggacgca ctggatcaga cgctcggcca
     6361 cttgcttgag caactcgtca cccatctggt gtcccagggt gtcgttgagg accttgaagt
     6421 tgtccagatc gatgaaaagc agtgcgccgt ggctgatgcg gcgcgcactg aactccatcg
     6481 cctgggtcag acggtccatc agtagccgcc ggttaggcag gcctgtgagc gaatcgtaaa
     6541 acgccagacg caaaatgtcg gcttccgcgc gtttgcgttc gctgatgtca cggccggtac
     6601 cccggtaacc cttgaagact tcgttgtcat cgaatatcgg ggcgccgctg atcgtcaccc
     6661 acataggcga cccatccgcc cgcaggcgct gcatctcgaa gtcatggaac acctcgtggg
     6721 cctccactgc cgcacggtgt gcagcccacc gctctggact gaccccttgc accccggaat
     6781 cccaccgcgt cttgcctacg taggacggag acggaagggc attggaggct tccagattgc
     6841 catccacacg ggtaaaccgg aactgctcgt cctgctccca gaaccagtcc gacgacaact
     6901 cggtcaatgc gcggaacctg gcctcgctct ggcgcatggc agcgagttgc tcggccacct
     6961 gctgcatgga ctcccgttcg gccgtaatgt cctgcacgat gccaaagtcg gctttgacca
     7021 caccgttttc aatttgccgg tgcatacgtg accgtatcca gacgacgcgg ccatccgggc
     7081 gatgccaacg gtactccacc aacttgccat ccatgcgctt gagcgcaccc gtgacgatgt
     7141 cccgatcctc gggcacgaca cttttcagat ggtcgcccac cggcgcatgc gttgtgtccc
     7201 caatgttggc cagcacgctg tagcctggcg acaactgaac ctcctcggca cccgggtaat
     7261 gcacccagtg gcccagtctg gccatggcct cggtcatttc atgcagcttg acctgccgat
     7321 tgagcttttc gttggtttgc gccagttcca gctcagtggc cttgagcgct tcctgagcca
     7381 tgagttcggc cgtcacatcg cgcaggtaaa tcaggacaaa cggaccagct ggcacatgga
     7441 ccagcacggc attgaggcgc accagccgcg ccacgccacc ggccatattc agcatcacat
     7501 ccaggtcagt cacccggcca ttggtcttga tgccatgcat cgcctgggcg cgcatgcctg
     7561 cgtctcgcca atggccaatc tccactgcag tgcggcccag cacctggtca cggggcaggc
     7621 ccgtgagctt gacccactcc tggttgactt ccacgatggc atcatcgcgg acacgggtaa
     7681 gtgcgatggc ttcggggctc tggtcgaaaa acgccttgaa catggcgccg acatccactc
     7741 caaaaaagtc cagacggcca tcacccaatg gcgcagtgcc acctgacgtg gtgttgtctg
     7801 gggtcttctc cactgatcgg gccggtttca ttccgtgttg tctgtctata gacacggata
     7861 atagctcggc aaactccatt ggtcaccacg gcgacgcaaa acccgctggc aatgcttcct
     7921 tttttcaggc tgcaacccat aattgccgcg aaaggaacca acgaccatga agaccaaaat
     7981 caccgaactg tttggcattg agcaccccat cattcagggg ggtatgcatt ttgtggggtt
     8041 tgccgaactg gcctccgccg tctccaacgc aggcggtctg ggcatgatta ccggcctgac
     8101 ccagcgctcc ccgaaggacc tggccaacga gatccgccgc tgccgcgaga tgaccgacaa
     8161 gccctttggc gtgaacctca cctttctgcc caccgtcagc tcgcccgatt accctggcta
     8221 catccaggcc atcatcgacg gcggcgtcaa ggcggtggag actgccggaa acaacccgca
     8281 aaaatacctg cccgcgctgc acgaagccgg catcaaggtc attcacaaat gcacctcggt
     8341 gcgccatgcg ctcaaggctg aatccattgg ctgcgatgcg gtcagcgtgg acgggtttga
     8401 gtgcggtggc cacccgggcg aggacgatgt gcccaacttc atcctgctgc cccgcgccgc
     8461 cgatgaactc aagattccat ttgtcgcatc gggcggtatg gccgatggcc gctctctggc
     8521 agccgccctg tgcctgggcg ccgagggcat caacatgggt acgcgcttta ttgccaccca
     8581 ggaggcgccc gtgcatgcca acgtcaagca ggccattgtg gctgccaccg aactcgacac
     8641 gcgcctggtg atgcgccccc tgcgcaacac cgagcgcgtc ctgcgcaacg ccgccgtgaa
     8701 ccgcctgc
//
LOCUS       DAOSJM010000051         8534 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7750_length_8534_cov_3.936785, whole genome shotgun sequence.
ACCESSION   DAOSJM010000051 DAOSJM010000000
VERSION     DAOSJM010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7750_length_8534_cov_3.936785"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="PKX82_02965"
     CDS             complement(<1..1099)
                     /locus_tag="PKX82_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOF51238.1"
                     /translation="MRQPSRAAFAVRACLLTTALFAGFAGSAFAQAKEIKIAHIYSKT
                     GPLEAYGKQTQTGFMMGLDYATGGTMTVAGKKLVVIEKDDQGKPDQGKSLLAAAYGDD
                     KVDVAVGPTASPVALAMLPVAEEYKKILLVEPAVADSITGEKWNKYIFRTGRNSSQDA
                     IANAVALDKQGVTIATMAQDSAFGRDGVKAFKEALKKARIVHEEFLPPATTDFTAGGQ
                     RLIDKLKDVPGRKVIFIIWAGGNPFKIADMDLKRHGIEIATGGNILPAMAAYKQFPGM
                     EGASYYYFGMPKNAVNEAMVSRHYSQFKAPPDFFTAGGFSAAMALVTALNNSKGDASA
                     NTLIKTMEGMSFDTPKGKMTFRKEDHQAMQSM"
     gene            complement(1250..2755)
                     /locus_tag="PKX82_02970"
     CDS             complement(1250..2755)
                     /locus_tag="PKX82_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOF51239.1"
                     /translation="MDKPDTLPRDAQSILELAARSMFELFASASEGMMLVDRQARVVW
                     INDQYRRFLPALGFEREEDFVGHPVSRVVQNTQMHQVLETGKPILIDLLTNRAGTFVV
                     SRIPLRDAAGQVIGALGIVLFDHPETTLQPLIEKFALLQRDLDDARRELASQRLQPAG
                     QRQSKYTFASFIGLSTAAVEVKRQARRAALSTSPVLLLGETGTGKELLAHAIHAASGR
                     ARGPFISVNIAAVPETLLESEFFGFAPGAFTGADRKGRDGKFKLAHGGTLFLDEIGDM
                     PMALQSKLLRALQEGEVEPLGSNKLIPVDIRVIAATSRDLHALVRKGLFREDLFYRLN
                     VLPIRVPPLRERREDIRALLEVLGEDVAVRSGMQAPELTPDALALLEAQPWRGNIREL
                     RNVLEQATMRSDTPRIEVPQIEAVLRESGLEPLEPQVSPERGPNAKAGVVQVPDDLLL
                     RPMSQQVAELESRAIAAAMRLHGGNKAAVAKALGISRATLYARLEMSEIYT"
     gene            complement(2823..4331)
                     /locus_tag="PKX82_02975"
     CDS             complement(2823..4331)
                     /locus_tag="PKX82_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOF51240.1"
                     /translation="MELWNHLLGGFATAGTPINLLWAFIGCAVGTAVGVLPGIGPAVA
                     VAMLLPITSQVDVTASMIFFAGIYYGAMYGGSTTSILLNTPGETGSMVTAMEGNKMAK
                     SGRAGAALATAAIGSFVAGSVATVLVTLFAPVMADFAVTLGPPEYFCLMLLALTTVSS
                     VLGQSTLRGITALFVGLAAGLIGMDQITGQVRYTGGVPELLDGIEIVLVAVGLFAVAE
                     ALYAVLYEGKTSESQNAMSRVHMTGKEWRRSWPAWLRGTVLGFPFGTIPAGGTEIPTF
                     LSYGLEKKLARDKSEFGTTGAIEGVAGPEAANNAAVTGTLVPLLTLGIPTSTTAAILL
                     GAFQNYGIQPGPQLFETSSSLVWALIASLYIGNVMLLVLNLPMVGLWVKLLKIPKPQL
                     YAGILIFATVGVYGMRQSAFDLVLLYAIGVLGVVMRRYDFPTAPVIVGMILGPLAEAQ
                     MRNALAIGEGKWTIFLERPMSLTLLAVVVAVLVVPRLFKQFSGPAIKDLPNH"
     gene            complement(4347..4856)
                     /locus_tag="PKX82_02980"
     CDS             complement(4347..4856)
                     /locus_tag="PKX82_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOF51241.1"
                     /translation="MSNAGRQKRQTLVGLGVLALGLGLGAGAYGIPSDAGYAGVGPNF
                     LPWVVSAVLVLCGGWVIREARSGGFRGMEPPSGAEDGHWPGFVWVSAGLLLNALLITS
                     VGFILSCTLCFTLAVRGLRTAEGRHDMSPRSWLVDIASGAAIAAPVYWMFTKLLAINL
                     PGLTATGWI"
     gene            complement(4856..5815)
                     /locus_tag="PKX82_02985"
     CDS             complement(4856..5815)
                     /locus_tag="PKX82_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOF51242.1"
                     /translation="MRRDVFLKSLAALAATGSLPLSALAATNLKMMIPANPGGGWDGT
                     GRAFGAALVDAKVVDTVQYENKGGAAGVIGLAQFYNASKGDPNALMVMGAVMVGGIIT
                     GKPQVSLDKLTPIARLTSEYNVFVVPANSPFKSMKDVVAAMQKDPGSVKWGGGSRGST
                     EHICASMLATKVNVDPKKVNYVAFRGGGEATAAILGGNVSVGGSGYSEFAEYIKSGKM
                     RPIGVTSDKRLPGIDVPTMKEQGFDVVLGNWRGVFGAAGITPAQRAELTEKVIKATQS
                     KSWKNSMEKNAWTPALLTGKAFDDFVGSDFASLRGIMHLSGML"
     gene            5909..6589
                     /locus_tag="PKX82_02990"
     CDS             5909..6589
                     /locus_tag="PKX82_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF51243.1"
                     /translation="MHLLLVEDDLALQSTLQRSLRRSGMRVEVCGDGAQALLHWQSLR
                     PDVVVLDLSLPDMDGLEVLGSARRSGLDTPVLILTARGTVGDKILGLNAGADDYLPKP
                     FDLDELEARVRALHRRRASQPRSLADESLRVGPLRFEPASGAMYLRQDILDLTPRELA
                     LLRALMTKPGHAVAKERLFEVVFPGEDHVQYEAIEVVVYRLRKKLADTGITLMTLRGL
                     GYLLKSDA"
     gene            6591..8012
                     /locus_tag="PKX82_02995"
     CDS             6591..8012
                     /locus_tag="PKX82_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOF51244.1"
                     /translation="MPATGRQISLRRQLLIGILLPIALFIVVDTVSLYRQALEAVNIA
                     YDRTLLASAKAIGELLEVEGEGDTARIRAQVPYSALEAFEADNRSRMSYRIADARGQW
                     IDGAQDLPAWTGTLPNQGPYAALVDFYDDQFRGDPVRVAVLLQPVATGSGRAMATIQV
                     AETLELRRTLARQILRDTLLRQAILIGVILAVVLVVVQRVTRPVRRLSDNLHKRTEDD
                     LTPIDSPDLPREMQPLTEATNQVMARLQHLLDHQKRFVRDAAHQLRTPLAVLKVQVQS
                     ARRGDMEPAEALRDIEVTVDRATTLANQMLSLAKVEQLRQQQDFAALEWAEVVRTLAL
                     DLSALIAEKDIDFELHTEPCTVHAHDWMLREMVRNLLHNAIRHTPEHGLLSVRIAPMD
                     GMAQLTIADSGSGIADALRMRLFQPFASGNTRSGTGLGLTIAREIILSLRGSIQLNNQ
                     TRDGQVIGLNAIVRLPLAALPSV"
     gene            8022..8423
                     /locus_tag="PKX82_03000"
     CDS             8022..8423
                     /locus_tag="PKX82_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOF51245.1"
                     /translation="MEKIRIDKWLWAARFFKTRSLATDEVQRGRVQMEQRDIKPSREV
                     QVGDTVTLWQGQVQRTVVVKGISGQRGSAPVAQQLYDETAESLTKRAQEAERRRLSPE
                     PAASIEHGRPTKRDRRNLEKGWNDRWSASLD"
BASE COUNT         1755 a         2682 c         2538 g         1559 t
ORIGIN      
        1 acatgctctg catggcctgg tggtcttcct tgcggaaggt catcttgccc ttgggtgtat
       61 cgaagctcat gccctccatg gtcttgatca gcgtgttggc agaggcatcg cccttgctgt
      121 tgttcagcgc agtcaccagg gccatggcgg cagagaagcc accggcagtg aagaagtccg
      181 gtggagcctt gaactggctg tagtggcgcg acaccatggc ttcgttgacc gcgttcttgg
      241 gcatgccaaa gtagtaatag ctggcacctt ccatgccggg gaactgcttg tatgcggcca
      301 tggcgggcag gatgttgccg cccgtggcaa tctcgatgcc gtggcgcttc aggtccatgt
      361 cagcgatctt gaaagggttg ccaccggccc agatgatgaa gatgaccttg cggccaggaa
      421 catccttgag cttgtcgatc aggcgctggc caccggcggt gaagtcggtg gtggcggggg
      481 gcagaaattc ttcatggacg atcctggctt tcttgagcgc ttccttgaac gccttgacgc
      541 catcacggcc aaaagcgctg tcctgcgcca tggtggcaat ggtcacgccc tgcttgtcca
      601 gcgccaccgc gttggcaatg gcgtcctgcg aactgttgcg gccggtacgg aagatgtatt
      661 tgttccactt ttcgccggtg atgctgtcgg ccacggcagg ctcgaccagc agaatctttt
      721 tgtactcttc ggccacgggc agcatggcca gtgccacggg cgaagcggtg gggccgacgg
      781 ccacatccac cttgtcgtca ccataggccg cggccagcag ggacttgccc tggtcgggct
      841 tgccctggtc atccttctcg atcacgacca gcttcttgcc cgcaacggtc atggtgccgc
      901 cggtggcgta gtccaggccc atcatgaagc cggtttgcgt ctgcttgccg taggcttcca
      961 gcggaccggt cttgctgtag atatgggcga tcttgatttc cttggcttgc gcaaaggcgc
     1021 tgccagcaaa accggcaaac agggcagtgg tcagcaggca ggcacgaacg gcaaaagctg
     1081 cccgggatgg ttggcgcatg gaggtctcct cttgtggtaa tgtcaacgaa gcaaattcgc
     1141 aagtgcattt ttgcaggttc catgccacat gctaagtgct tgatttcatt gagccgacat
     1201 ctcaatatgt ctgaaataca aacaaattac catgtgctga ttttatacac tatgtatata
     1261 tttcagacat ttccagccgt gcatacagcg tagcgcgcga aatgcccaaa gccttggcga
     1321 ccgccgcctt gttgccgcca tgcaaccgca tggccgcggc aatggcgcgc gactccagct
     1381 cagcaacctg ctgcgacatg ggtcgcaaca acagatcgtc aggcacctgg acaacgcccg
     1441 cctttgcatt cgggccgcgc tccgggctga cctgtggctc caggggttcc agcccggatt
     1501 cacgcaacac cgcctcgatc tgcggcacct caatgcgcgg cgtatcgctg cgcatggtgg
     1561 cctgctccag cacgttgcgc agttcgcgga tgttgccgcg ccagggctgg gcttccagta
     1621 acgccagagc gtcgggcgtc aattccggag cttgcatgcc gctgcgcacc gccacgtctt
     1681 cccccagcac ctccagcagc gcacgaatgt cctcgcggcg ctcgcgcagg ggcggcacac
     1741 ggatgggcag cacattgagg cgataaaaca ggtcttcccg aaacaggcct ttgcgcacca
     1801 gcgcatgaag atcgcgcgag gtggctgcaa tcacgcgtat atccaccgga atgagcttgt
     1861 tggagcccag cggctccacc tcgccttcct gcaacgcccg cagcaacttg gactgcaatg
     1921 ccatgggcat gtcgccaatc tcgtccagaa aaagcgtgcc accgtgcgcc agcttgaact
     1981 tgccatcgcg ccctttgcgg tccgcgccgg taaatgcacc gggtgcaaag ccgaagaact
     2041 cggactccag caacgtctcg ggcacggcag caatgttcac actgatgaac ggtccgcgcg
     2101 cccggccgga cgccgcatgg atggcgtggg ccagcaactc cttgccagtg ccggtttcgc
     2161 ccagcagcag cacgggactg gtggagagcg ccgcacgccg tgcctggcgc ttgacctcga
     2221 ccgcggcggt actcaggcca atgaagctgg caaaggtgta cttggactgg cgttgccccg
     2281 caggctgcag gcgctgcgag gccagttcac gccgcgcatc gtccagatcg cgctgcagca
     2341 aggcgaattt ttcaatcagg ggctgcaggg tggtttcggg gtggtcaaac agcacgatgc
     2401 ccaaagcgcc aatcacctgc cccgctgcat cgcgcaacgg aatgcggctg accacaaagg
     2461 taccagcccg gttggtcaac aggtcgatca ggatgggctt gccggtttcc agcacctggt
     2521 gcatctgggt gttttgcacc acccgggaca ccgggtgccc gacaaagtct tcctcgcgct
     2581 caaaccccag cgcaggcagg aagcggcggt actggtcgtt gatccagacc acccgggcct
     2641 gccggtccac cagcatcatg ccttcgctgg cgctggcgaa cagttcaaac atggaccgcg
     2701 cggccagctc cagaatgctc tgggcgtcgc ggggcagggt gtcgggttta tccatacatg
     2761 gatgctacca aattagtagc tggctacgca cactgcgtgc gggctagagg ccaatttcat
     2821 tcctaatggt taggtaaatc cttgatggcg ggccccgaga actgtttgaa caggcgtggt
     2881 accaccagga ctgcgaccac gaccgccagc agcgtgagcg acataggacg ctccagaaag
     2941 atggtccact tgccttcgcc aatggcgagg gcattgcgca tctgggcttc ggccaaaggg
     3001 cccagaatca tgcccacgat gactggcgcc gttggaaagt catagcgtcg catcaccaca
     3061 cccagcacgc caatggcgta cagcagcacc agatcgaaag cgctctggcg catgccgtag
     3121 acacccaccg tcgcaaaaat gaggataccg gcatacaact gcggcttcgg gatcttgagc
     3181 agcttcaccc acaaccccac catcggaagg ttaagcacca gcagcatcac gttgccaatg
     3241 tacagcgatg caatcagcgc ccacaccagc gaagaggaag tttcaaacag ctgcggtccc
     3301 ggctggatgc cgtagttctg gaaggctccc agcagaatgg ccgccgtcgt ggaggtggga
     3361 atccccagtg tgagcagcgg caccagcgta ccagtgacag ccgcgttgtt ggcagcttca
     3421 ggcccggcaa caccttcaat agcgcctgtg gttccaaatt cgcttttgtc cctggccagt
     3481 tttttctcca gaccatagct caggaatgtg ggaatctcgg taccaccagc gggaatggtg
     3541 ccaaacggaa aaccaaggac tgtgccacgc agccaggccg gccaggagcg gcgccactct
     3601 ttgccggtca tgtgcacccg gctcatggca ttttgcgatt cgctggtctt gccctcgtaa
     3661 agtacggcat acagtgcttc agccacggca aacaggccta ccgccaccag cacaatctca
     3721 atgccgtcca gcagttcagg gaccccaccg gtatagcgca cctggccggt aatctggtcc
     3781 ataccgatca atccggccgc cagaccgaca aacagcgccg taatgccacg caatgtgctt
     3841 tgccccaaca ccgaactcac cgtggtaagt gccagcagca tcaggcaaaa atactcgggc
     3901 gggccgagtg tgacggcaaa gtcagccatg accggcgcga acagcgtcac cagcacggta
     3961 gcaacactgc cagccacaaa agaaccgatg gcggccgtgg ccagcgcagc gcctgcgcgg
     4021 ccgctcttgg ccatcttgtt gccctccatg gcggtcacca tacttccggt ttcgcccggg
     4081 gtattcagca aaatcgacgt tgtggagccg ccgtacatgg caccgtagta gataccggca
     4141 aagaaaatca tcgaggcagt gacatccacc tggctggtga tgggcaacag catggccact
     4201 gccacggctg gcccgatgcc aggcaagacc cctaccgccg tgccgacggc acagccaata
     4261 aaagcccaca gcagattaat gggggtgccg gccgtggcga agcctcccag caagtggttc
     4321 cagagttcca ttcggggtct ttcagatcag atccagccag tggctgtgag gcccggcaaa
     4381 ttgatggcaa gcagcttggt gaacatccag tacaccggcg ccgcaatggc tgcgccactg
     4441 gcaatatcca ccagccagct gcgtggggac atgtcatggc gcccttctgc agtacgcaaa
     4501 ccacgtactg ccagggtaaa gcacaaggtg cagctcagga tgaagccaac actggtaatc
     4561 agcaaggcat tgagcagcag gccggccgag acccagacaa agcccggcca atgtccgtct
     4621 tctgccccgg aaggaggctc catgccccgg aatcctccgc tgcgcgcttc gcgaatgacc
     4681 cagccaccgc acagcaccag aacggcactg accacccagg gcaggaagtt gggccctacg
     4741 ccggcatagc cggcgtccga cgggatgcca taggcccccg cccccagacc cagccccagg
     4801 gccagcacgc ccaacccgac cagggtctgg cgcttttgtc ggccggcgtt ggacatcaca
     4861 gcattccgga aaggtgcatg atgccgcgca ggctggcgaa gtcgctgccc acgaaatcgt
     4921 caaaggcctt gccggtgagc agcgcgggag tccaggcatt tttttccatg gaatttttcc
     4981 aggatttgct ctgggtcgcc ttgatgactt tttcagtcaa ctccgcacgt tgcgccggag
     5041 tgatgccggc cgcgccaaac acgccacgcc agttgcccag cacgacatca aagccttgct
     5101 ctttcatggt gggcacgtcg atgccgggca aacgcttatc ggacgtcacg ccaatggggc
     5161 gcatcttgcc ggacttgatg tattcagcaa actcgctgta gccactgcct ccgacagaca
     5221 cgttgccacc caggatggcc gccgtggctt cgccgccgcc gcggaaggcg acatagttca
     5281 ctttcttggg gtccacattg accttggtgg ccagcatgga agcgcagata tgctccgtag
     5341 agccgcgaga accgccgccc cacttgacac tgccaggatc cttttgcatg gcagccacca
     5401 catccttcat gctcttgaag ggcgagttgg cgggtaccac aaacacgttg tactcgctgg
     5461 tcaggcgtgc aatcggggtc agcttgtcca gcgacacctg cggcttgcca gtaatgatgc
     5521 cgcccaccat cacggcgccc atcaccatca gcgcattggg atcacctttg ctggcgttgt
     5581 aaaactgggc cagaccaatg acaccggcag caccaccctt gttttcatac tgaacggtgt
     5641 caaccacctt ggcatcgacc agggctgcgc caaaggcacg tccggtgcca tcccagccac
     5701 cacccgggtt ggcaggaatc atcatcttga ggttggtggc tgccagagcg ctcagcggca
     5761 ggctaccggt agcggccaac gcggccaggg acttcaggaa tacatcacga cgcataagat
     5821 ttctcctcgg gttgaaaacc accctatgat gcggcggccg tctgtcattc acctgtccag
     5881 tttcctaggg ataaccctca gacacaccat gcacctgctt ctcgtcgaag acgatctggc
     5941 actccagtcc accttgcagc gcagcctcag gcgcagcggc atgcgggtgg aagtctgtgg
     6001 cgacggcgca caggcgctgc tgcactggca aagcctgcgg ccggacgtgg tggtgctgga
     6061 cctgagtctg ccggacatgg acgggctgga agttctgggc agcgcacgcc gatcgggcct
     6121 ggacacgccg gtactgatcc tcacagcacg cggcaccgtg ggcgacaaga ttctgggcct
     6181 gaacgcagga gccgatgact atctgcccaa acccttcgat ctggacgagc ttgaggccag
     6241 ggtacgtgca ttgcaccgcc gccgcgcaag ccagccccgt tcgctggcag atgagtccct
     6301 gcgcgtaggc cccctgcgat ttgaaccggc cagtggtgcc atgtacctgc gccaggacat
     6361 tctggacctg acaccgcgtg aactcgccct gctacgcgca ttgatgacca agccgggcca
     6421 tgctgtggcc aaggaacgcc tgtttgaagt ggtattcccg ggtgaggacc acgtgcagta
     6481 cgaggccatc gaagtcgtgg tgtaccggct gcgcaagaag ctggccgata ccgggatcac
     6541 actcatgacg ttgcgtggcc tgggctacct gctgaagtcc gatgcctgac atgccggcca
     6601 caggacgcca gatttctctg cgacgccagt tgctcattgg cattctgctg ccgattgcgc
     6661 tgttcattgt ggtggatacg gtcagcctgt accgacaggc gctggaggcg gtgaatatcg
     6721 cctacgaccg cacactactg gcttcggcca aggccattgg cgaattgctg gaagtcgaag
     6781 gtgagggaga cacggcacgc atccgggccc aagtgcctta ttccgccctg gaggcgtttg
     6841 aagccgacaa ccgcagtcgc atgagctacc gcatcgccga tgcccggggg caatggattg
     6901 atggtgccca ggacctgccg gcctggaccg gcacccttcc caaccaggga ccttacgcag
     6961 ccctggtgga cttttacgat gaccagttcc gtggtgaccc ggtgcgcgta gccgtcctgc
     7021 tgcagccggt agcgacgggc agtggccgtg ccatggccac catccaggtg gccgaaacgc
     7081 tggaactgcg acgcacgctg gcgcgtcaaa ttctgcgtga caccctgctg cgccaggcga
     7141 tcctgatcgg tgtcattctt gcagtcgtgc tggtggtggt tcagcgcgtc acccgcccag
     7201 tgcgcagact cagcgataac ctgcataaac gcaccgagga tgacctcaca ccaatcgact
     7261 ctccggacct gccgcgcgaa atgcaaccgc tgaccgaagc caccaaccag gtaatggcaa
     7321 ggctgcaaca cctgctcgac caccagaagc ggtttgtgcg ggatgcggca caccagttgc
     7381 gtacgccgct ggcggtactc aaggtgcaag tgcaatcggc ccgacgcggc gacatggaac
     7441 cggcggaagc attgcgcgac atcgaggtga ctgtagaccg tgcaaccacg ctggccaacc
     7501 agatgctctc cctggcaaaa gtggagcagt tgcggcagca gcaggacttt gccgcgctgg
     7561 agtgggctga agtggtgcgc acgctggcac tggacctgtc tgccctgatc gcagagaaag
     7621 acatcgattt cgaactgcac accgagccgt gcacggtcca tgcccatgac tggatgctgc
     7681 gcgaaatggt gcgcaattta ctgcacaacg ccatccgcca tacgccggag cacggactgc
     7741 tgagtgtgcg tattgccccg atggacggca tggcgcaatt gaccattgcc gactcgggga
     7801 gtggcattgc agacgccctg cgcatgcgac tgttccagcc gttcgcctcc ggcaacacgc
     7861 gctccggtac cgggctgggt ctgacaattg cacgcgaaat tatcctatcg ctccgtggca
     7921 gcatccagct caacaaccag acccgcgatg ggcaggttat cggactgaac gccattgtcc
     7981 gcctgccact ggcagccctc ccttccgtct gaatggactc catggaaaaa atccgtatcg
     8041 acaagtggct gtgggccgca cgcttcttca aaacccgatc cctggccacc gacgaagtgc
     8101 agcgcggccg cgtgcagatg gaacagcgcg acatcaaacc gtcgcgcgag gtacaggtgg
     8161 gcgataccgt gacgctgtgg cagggccagg tccaacgcac ggtggtggtc aagggcatca
     8221 gcgggcagcg cggttccgct ccggtggcac agcagctcta cgacgaaaca gccgagagtc
     8281 tgaccaaacg ggcgcaggaa gccgaacgcc ggcgactcag cccggagccc gccgcatcca
     8341 tcgaacacgg ccgccccacc aagcgtgacc ggcggaatct ggaaaaggga tggaacgacc
     8401 gctggagcgc gtcactggac tgaacaagaa gcaaacctgg aaatagaaaa agcccgaggg
     8461 ttactcgggc tttttgcatt tggcggaaac ggagggattc gaaccctcga tgaggctcta
     8521 caccccatac tccc
//
LOCUS       DAOSJM010000052         8518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7769_length_8518_cov_4.373390, whole genome shotgun sequence.
ACCESSION   DAOSJM010000052 DAOSJM010000000
VERSION     DAOSJM010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7769_length_8518_cov_4.373390"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1873
                     /locus_tag="PKX82_03005"
     CDS             29..1873
                     /locus_tag="PKX82_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOF51246.1"
                     /translation="MRPSGYNASAISSAPANALPKEKSDWDTLKRLFPYLWVYKWRVM
                     AALAFMVGAKFANVGVPLLLKNLVDAMTIQPGDPAALLVVPVALLIGYGALRLSTSVF
                     TELRELVFAKATAGASRSISLQVFRHLHALSLRFHLERQTGGLTRDIERGTRAVNSLI
                     SYSLYSIVPTLIEVALVLTLLAVKFDVWFAWITIGALVFYIAFTITVTEWRTQFRKTM
                     NELDSTANSRAIDSLLNFETVKYFNNEEFEAKRYDYNLERYRKASVKSQTTLSLLNSG
                     QQFIIAVGLVIMLWRATLGVAEGRMTLGDLVMVNAFMIQLYIPLNFLGVIYREIKQSL
                     TDLEKMFGLLEREREIADAPQAQPLQLRADGSDASVRFEDVVFAYDPARTILHGISFE
                     IPAGKTVAVVGPSGSGKSTLARLLFRFYDVGQGRILLGGQDIRQVTQASVRQSIGIVP
                     QDTVLFNDTVEYNIAYGRPGASRAEVEGAARAAHIHDFIAGTPKGYDTMVGERGLKLS
                     GGEKQRVAIARTLLKNPPIVIFDEATSALDSANERAIQAELQGVSQNKTTLVIAHRLS
                     TVVDAHEILVMDSGRIVERGTHQALLAANGRYAQMWNLQQQSSATAAA"
     gene            1899..2279
                     /locus_tag="PKX82_03010"
     CDS             1899..2279
                     /locus_tag="PKX82_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOF51247.1"
                     /translation="MSTSQGTMDYLLDQLKSAGPVSSRKMFGEYCLYYAGRPVGLVCD
                     EQLYLKPTDAGRALMREVREGAPYPGARMHLLVTADDWDDHHLLGQLVRATFDALPPP
                     KVPKPRKPAAKKSASTGTTRTKAK"
     gene            2285..2893
                     /locus_tag="PKX82_03015"
     CDS             2285..2893
                     /locus_tag="PKX82_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51248.1"
                     /translation="MEIVLVLAAFTAFTVFKSREQKRRIALLASYLGRYEIEKLMETL
                     MDGYLRALGEGSAERQAQVWEHLHAQEDKLRDQFQQFAAAFADVWADSARVSTLPIAF
                     PWADKLFPAQAFDLRHALQIHARGIDAAVSNVRQDAPKDRAFTLTAELMLMQHTCHWF
                     CRSKNVASARLLARHQTHYHQVLAAVSEKTRMQYTQLIQHKA"
     gene            2925..3890
                     /locus_tag="PKX82_03020"
     CDS             2925..3890
                     /locus_tag="PKX82_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOF51249.1"
                     /translation="METKWLEDFVSLAETRSFSRSAQLRHVTQPAFSRRIQSLEAWAG
                     TDLVDRSSYPTRLTAAGETLYEQSLEMLQALQSTRAMLRGHTTAGQDVIEFAVPHTLA
                     FTFFPAWVSDLRENFGPIKSRLIALNVHDAVMRLVEGSCDLLIAYHHPSQPYQFDTDR
                     YEMVSLGEEVVAPYVKPGPDGAPLYALPGKVGQPLPYLAYAPGAYLGQMVDLILKQSA
                     IPIHFDRVYETDMAEGLKAMALEGHGVAFLPHSAVKKDLRAKKLVSAMPAGTQDLQIT
                     MEVRAYRERPLGKEGARSGTKAPKSAAQALWNYLAAGAAAGTIRE"
     gene            3887..4939
                     /gene="pyrC"
                     /locus_tag="PKX82_03025"
     CDS             3887..4939
                     /gene="pyrC"
                     /locus_tag="PKX82_03025"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOF51250.1"
                     /translation="MNPMQNSQTNTLTITRPDDWHLHVRDGAALNTVVPHTAAQFGRA
                     IIMPNLKPPVTTAEQALAYKARIQAAVPAGVQFEPLMTLYLTDNLPADEIARARDAGV
                     VAVKLYPAGATTNSDAGVTDLRKTYKTLEAMQKAGLLLLVHGEVTSSDIDLFDREAVF
                     IDTQLIPLRRDFPELKIVFEHITTQEAAQYVTESDRFTAASITAHHLLYNRNAIFTGG
                     IRPHYYCLPVLKRETHRLALVQAATSGNAKFFLGTDSAPHPAHLKEHASGCAGCYTAH
                     AAMELYAQAFDAAGALDKLEGFASFHGADFYGLPRNTGTISLRRESWTVPETFAFGEA
                     QLKPLCGGDALPWRVV"
     gene            5026..5775
                     /locus_tag="PKX82_03030"
     CDS             5026..5775
                     /locus_tag="PKX82_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HOF51251.1"
                     /translation="MSLASVEWSAPWLAPYAELGQRVAGRVAAGLSSAEALNGALAPQ
                     ATPVRFVPQSDLPAGVAYESYIFDSDCCPTREGLHDFFNGLAWLHFPQTKRRLNHLHV
                     AQIAQTGIQPVRGPARDALTVFDENAAFFQGPDALWDALAAKDWHSLFVTLRPQWQEA
                     HLVLFGHALLEKLVYARKSITAHVFRAQAATNSIADMDAWVAAQLTAEMLATKPFAHL
                     PVLGVPGWWPENADPAFYADTSVFRPPKTIN"
     gene            5868..6740
                     /gene="htpX"
                     /locus_tag="PKX82_03035"
     CDS             5868..6740
                     /gene="htpX"
                     /locus_tag="PKX82_03035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOF51252.1"
                     /translation="MKRIFLFVMTNLAVVVVLGIVASLTGANRFIGANGMNLGTLMVF
                     ALIMGFGGAIISLLISKPVAKWSSGVRVINQPQNADEAWIVDTVRKFADKAGIGMPEV
                     GIFEGAPNAFATGAFKNSALVAVSTGLLQGMTREEVEAVIGHEVAHIANGDMVTMTLI
                     QGVMNTFVVFLSRVIGYAVDSFLRKGDSENSGPGIGYMITTIVLDIVLGFAAAIVVAW
                     FSRHREFRADAGAAQLMGRKQPMINALARLGGMTPGELPKSVAAMGIAGGIGQLFSTH
                     PPIEERIAALQNAQ"
     gene            complement(6762..8246)
                     /gene="ubiD"
                     /locus_tag="PKX82_03040"
     CDS             complement(6762..8246)
                     /gene="ubiD"
                     /locus_tag="PKX82_03040"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041322210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HOF51253.1"
                     /translation="MAYHDLRDFMAQLEQAGELRRVTQPVSPHLEMTALCDRVLRAAG
                     PALLFEHPTGHTMPVLGNLFGTPERVARAMGVNSISGLRPFGELLASLKEPEAPKGFK
                     DMVGMRGLLKTLWNMAPKERSSAPCQEVVWEGPDADLARLPIQHCWPGDVAPLITWGL
                     VITRGPHKARQNLGIYRQQVLSRNQLIMRWLAHRGGALDFRDHGQLHPGQPYPVAVAL
                     GADPATILGAVTPVPDSLSEYQFAGLLRGARTEVVRALGSSLQVPASAEIVLEGHIYP
                     DANHASGYQHALEGPYGDHTGYYNEQAEFPVFTVDRISLRRDPIYHSTYTGKPPDEPA
                     VLGMALNELFIPLLQRQFPEITDFYLPPEGCSYRMALVQIKKAYPGHARRVMMGVWSH
                     LRQFMYTKFIVVVDDDVDVRDWKEVIWALTTRVDPVRDTMMVENTPIDYLDFASPVSG
                     LGSKMGLDATNKWPGETQREWGRTITMQAEVQARMDQLYQDLGL"
     gene            complement(8256..>8518)
                     /locus_tag="PKX82_03045"
     CDS             complement(8256..>8518)
                     /locus_tag="PKX82_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007581005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF51254.1"
                     /translation="ARARVKDLLDRVGLPTDASHRYPSEFSGGQRQRIAIARALALEP
                     RLIVCDEPVSALDLSTQARVLDLLDLPHTLAPRWQGQTPANP"
BASE COUNT         1561 a         2699 c         2688 g         1570 t
ORIGIN      
        1 tgggtaataa ccacctgaat tgtcgtacat gcgcccctct ggctacaacg cctctgccat
       61 ttcttccgca cctgcgaacg cgctgcccaa ggagaaatcc gattgggaca ccctcaagcg
      121 cctgttcccc tacctctggg tctacaagtg gcgggtgatg gctgcgctgg ccttcatggt
      181 gggggcgaag tttgccaacg tcggtgtccc cctgctgctc aagaacctgg tggacgccat
      241 gaccatccag cccggcgacc cggccgcctt gctggtggtg ccggtggcgt tgttgatcgg
      301 ttatggcgca ctgcgcctgt cgacctcggt gttcaccgag ttgcgcgagc tggtgtttgc
      361 caaggccacg gcgggggcat cgcgcagcat ctctctgcag gtgtttcggc atctgcacgc
      421 cctgagcctg cggttccatc tggagcgcca gaccggcggc ctgacccgcg acattgagcg
      481 cggcacgcgg gcggtcaatt ccctcatcag ctactcgctg tacagcatcg tgcccacgct
      541 gattgaggtg gcgctggtgc tgacgctgct ggcggtcaag ttcgacgtgt ggtttgcctg
      601 gatcaccatt ggggcactgg tgttctacat tgcgttcacc attaccgtga ccgagtggcg
      661 cacccagttt cgcaagacca tgaacgagct ggattccacc gccaacagcc gtgccatcga
      721 ttcgctgctg aacttcgaga ccgtcaagta tttcaacaac gaagagtttg aagccaagcg
      781 ctacgactac aacctggagc gctaccgcaa ggcctcggtc aagagccaga ccacgttgag
      841 tctgctcaac tcgggccagc agttcatcat cgccgtgggg ctggtcatca tgctgtggcg
      901 tgccaccctg ggtgtggccg aagggcgcat gacgctgggc gatctggtga tggtgaacgc
      961 cttcatgatc cagctctaca ttccgctgaa ttttctgggc gtgatctacc gcgagatcaa
     1021 gcagagcctg accgacctgg aaaaaatgtt tggcctgctg gagcgcgagc gtgaaatcgc
     1081 cgatgcacca caggcgcagc ccctgcaact gcgtgccgat gggtcggacg ccagcgtgcg
     1141 ttttgaggat gtggtgtttg cctacgaccc ggcccgaacc atcctgcacg gcatttcgtt
     1201 cgagattcct gcgggcaaga cggtggcggt ggtggggccc agtggttcgg gcaagtccac
     1261 gctggcgcgg ctgctgttcc ggttttacga cgtgggccag gggcgcatcc tgctgggcgg
     1321 gcaggatatc cgccaggtca cgcaggccag cgtgcggcag tccatcggca tcgtgccgca
     1381 ggatacggtg cttttcaacg acacggtgga atacaacatt gcctatggcc gccctggcgc
     1441 cagccgggcc gaggtggagg gagccgcgcg ggccgcgcac atccacgact tcattgccgg
     1501 tacgcccaaa ggctacgaca ccatggtggg tgagcggggc ctgaagctgt cgggcggcga
     1561 gaagcagcgc gtggccattg cgcgcactct gctcaagaat ccgccgattg tgatttttga
     1621 cgaagccacc agtgcgctgg acagcgccaa cgagcgggcc atccaggctg aactgcaggg
     1681 cgtgtcgcag aacaagacca cgctggtgat tgcccaccgc ctgtccacgg tggtggatgc
     1741 gcatgagatt ctggtgatgg attcgggccg tattgtcgag cgcggcaccc accaggcgct
     1801 gctggccgcc aacgggcgct atgcccagat gtggaacctg cagcagcaaa gcagcgccac
     1861 cgcggcggct tagccagaca acgcaaagga gagggagcat gtccacgtca caaggcacca
     1921 tggattacct gctggaccag ctcaaatcgg ccgggccggt gtcgtcgcgc aagatgtttg
     1981 gcgagtactg cttgtactac gcgggccggc ctgtggggct ggtgtgcgac gagcagcttt
     2041 acctcaaacc taccgatgcc ggccgtgcgc tgatgcgcga agtgcgcgag ggcgcaccgt
     2101 atcccggcgc acgcatgcac ctgctggtca cggccgacga ttgggacgac caccatctgc
     2161 tgggacaatt ggtgcgcgcc accttcgatg cgttgcctcc gcccaaggtc cccaaacccc
     2221 gcaaacccgc tgccaagaaa agtgccagca ccggtacaac ccgcacaaaa gcaaaatagc
     2281 ctgtatggaa atcgttctcg ttctggcggc attcaccgcg tttaccgtgt tcaagtcccg
     2341 cgagcagaag cggcgcattg ccctgctggc aagttacctg gggcggtacg aaatcgaaaa
     2401 attgatggaa accctgatgg atggctacct gcgcgcgctg ggagaaggca gcgccgagcg
     2461 ccaggcccag gtctgggagc acctgcatgc gcaggaagac aagctgcgcg accagtttca
     2521 gcagttcgcc gcagcgtttg ccgatgtatg ggccgacagt gctcgggtca gcacgctgcc
     2581 cattgccttc ccctgggccg acaagctgtt tccggcacag gcctttgatt tgcgccacgc
     2641 cctgcagatc cacgcacgcg gcattgacgc cgccgtgtcc aatgtgcggc aggatgcacc
     2701 caaggatcgg gctttcaccc tgacggccga actgatgctg atgcagcaca cctgccactg
     2761 gttttgccgg tccaaaaacg tcgcatccgc acgcttgctg gcacgccacc agacgcacta
     2821 ccaccaggtg ctggccgcgg tgtcggaaaa gacgcgcatg cagtacaccc agctcatcca
     2881 gcacaaggcc tgagtgccca gggccacctg aagataatcg gggcatggaa accaaatggc
     2941 ttgaagactt tgtcagcctg gctgaaacgc gcagtttcag ccgctcggcc cagctgcgcc
     3001 atgtgacgca gccggcattc tcccggcgca tccagtcgct ggaggcctgg gcaggaactg
     3061 atctggtgga ccgcagctcc taccccacgc ggctcaccgc tgcgggagag acgttgtacg
     3121 agcagtcgct ggaaatgctg caggcgctgc agtccacgcg cgccatgttg cgcggccaca
     3181 ccaccgctgg ccaggatgtg atcgaattcg ccgtaccgca cacgctggcg ttcactttct
     3241 tcccggcgtg ggtgtccgat ctgcgcgaga acttcgggcc catcaagagc cgcctgattg
     3301 cactcaatgt gcacgatgcg gtcatgcgtc tggtggaggg cagctgcgat ctgctgattg
     3361 cctaccacca cccctcgcag ccctaccagt tcgataccga ccgctacgaa atggtcagcc
     3421 tgggcgaaga ggtggtggca ccctacgtca agcccggccc ggacggtgca ccgctgtatg
     3481 cgttgcccgg caaggtcggc cagccgctgc cttacctggc ctatgcgccg ggcgcctacc
     3541 tggggcagat ggtggacctg atcctcaagc agtccgccat accgatccac tttgaccggg
     3601 tgtacgagac cgacatggcc gagggcctga aggccatggc gctggagggc cacggcgtgg
     3661 cattcctgcc ccacagcgcg gtcaagaaag acctgcgcgc caaaaaactg gtcagcgcca
     3721 tgcccgctgg cacgcaggac ctgcagatca ccatggaagt gcgcgcctac cgcgaacgcc
     3781 cgctgggcaa ggaaggcgcc cgcagcggaa ccaaggcccc caaaagtgcc gcgcaggccc
     3841 tttggaacta cctcgcagca ggcgctgctg cggggacaat acgcgaatga acccgatgca
     3901 aaactcccag accaacaccc tgaccatcac ccgccctgac gactggcact tgcatgtgcg
     3961 tgacggagct gccctgaata ccgtggtgcc acacaccgcc gcccagtttg gccgcgccat
     4021 catcatgccc aacctcaagc cgccagtgac cacggccgag caggcgctgg cctacaaggc
     4081 gcgcatccag gccgccgtgc cagcgggcgt tcagtttgag ccgctgatga cgctgtacct
     4141 caccgacaac ctgcctgcgg acgaaatcgc ccgcgccaga gacgctggcg tggtggccgt
     4201 caagctctac cccgcaggcg ccaccaccaa cagcgatgct ggcgtgaccg acctgcgcaa
     4261 gacctacaag acgttggagg ccatgcagaa ggccggcctg ttgctgctgg tccatggcga
     4321 agtgacttcc agtgacatcg acctgttcga ccgcgaggcg gtgttcatcg acacccagct
     4381 gattcccttg cgccgtgact tccccgagct caagatcgtg ttcgagcaca tcacgaccca
     4441 agaagccgcg caatacgtga ccgagagcga ccgcttcacc gccgccagca tcacggccca
     4501 ccacctgctc tacaaccgca acgccatctt caccggcggt atccgtccgc attactactg
     4561 cctgccggtg ctcaagcgcg aaacgcaccg cctggcgctg gtgcaggcgg ctaccagtgg
     4621 caacgccaaa ttctttctgg gcaccgacag tgcaccgcat ccggcccacc tgaaagagca
     4681 cgccagcggt tgcgccggct gctacaccgc gcacgcggcc atggagctgt atgcgcaggc
     4741 cttcgatgcg gctggggcgc tggacaagct cgaaggcttc gccagtttcc atggcgctga
     4801 cttttatggc ctgccgcgca acaccggcac catctccctg cgccgcgagt cctggaccgt
     4861 gccggagaca tttgcttttg gcgaggcgca gttgaaaccg ctgtgtgggg gtgacgcact
     4921 gccttggcgg gttgtctaag gacatggaca gcagagcgct ctgctccgtg tcccccgccc
     4981 gctgcgcggg ctcctccttt acctgcgcag aacgccctgc cgcccatgtc cttagcctct
     5041 gttgaatggt ctgcgccttg gctggcgccg tatgcagagc tgggccagcg cgtggccggt
     5101 cgggtggcgg caggcctgtc cagtgccgag gcgttgaatg gcgcactggc gccccaagcg
     5161 actccagtgc gttttgtgcc gcaaagtgat ctgcctgcgg gggtagcgta tgaaagctat
     5221 atttttgata gcgactgctg cccaacccgc gagggcttgc acgacttttt caatggactg
     5281 gcgtggctgc acttcccgca gaccaagcgt cgcctgaacc acctgcatgt ggcccagatt
     5341 gcacagaccg gtatccagcc ggtgcgcggc ccggcgcggg atgcgctcac cgtgttcgac
     5401 gaaaacgccg cttttttcca ggggccggat gcgctgtggg acgcgctggc cgccaaagac
     5461 tggcacagcc tgtttgtgac cctgcgcccg cagtggcaag aggcgcatct ggtgctgttt
     5521 ggtcacgccc tgctggaaaa gctggtttat gcacgaaaat cgatcacagc ccacgtattt
     5581 cgtgcgcagg cagctactaa ttcaatagca gatatggacg cctgggtggc tgcacaactg
     5641 acggccgaga tgctggcgac caagcccttt gcccacctgc cggtgctggg ggttcctggt
     5701 tggtggccag agaacgcgga cccggcgttt tatgccgata ccagcgtgtt tcggccaccg
     5761 aaaaccataa attaattggg agtttggctg gagaaatctt gattttggcc aatcagcccc
     5821 taccattccg ggcaacgcca actcaacagg cgacactgaa ggaaaccatg aaacgcattt
     5881 tcttgtttgt aatgaccaac ctcgctgtgg ttgtggtgct gggcattgtg gccagcctga
     5941 ccggcgcgaa ccgctttatt ggcgccaatg ggatgaattt gggcaccctg atggtgttcg
     6001 cgctcatcat gggctttggt ggcgccatca tttcgctgct gatcagcaag ccggtggcca
     6061 agtggtcgtc gggtgtgcgg gtcatcaatc agccgcaaaa cgccgacgaa gcctggattg
     6121 tggacaccgt gcgcaagttc gccgacaagg ctggcatcgg catgcctgaa gtcggcattt
     6181 ttgaaggcgc acccaacgcg tttgccaccg gtgcgttcaa gaactcggcc ctggtggccg
     6241 tctccaccgg cttgctgcag ggtatgaccc gcgaggaagt cgaagccgtg attggccatg
     6301 aggtggcaca catcgccaac ggcgacatgg tcaccatgac gctgatccag ggcgtgatga
     6361 atacttttgt ggtgtttttg agccgggtga tcggctacgc ggtggacagc ttcctgcgca
     6421 agggcgacag cgagaacagc ggccccggca ttggctacat gatcaccacg atcgtgctcg
     6481 acatcgtgct gggttttgct gcggccatcg tcgtggcctg gttctcacgc caccgcgaat
     6541 tccgtgccga tgcaggcgct gcccaactga tgggccgcaa gcaacccatg atcaacgccc
     6601 tggcccgcct gggtggcatg acaccgggcg agctgcccaa gagcgtggcg gccatgggca
     6661 ttgccggcgg cattggtcag ctgttctcaa cccacccgcc catcgaagag cgcatcgctg
     6721 cgctgcagaa cgcccagtaa agcggactgg ggtggggctg tctatagccc cagatcctgg
     6781 tagagctggt ccatgcgggc ctgcacctcg gcctgcatgg tgatggtgcg gccccattcc
     6841 ctttgtgttt ccccaggcca tttgttggtg gcatccagcc ccatcttgct gcccaggcca
     6901 ctgactggcg aggcaaagtc caggtagtcg atcggcgtgt tctccaccat catggtgtcg
     6961 cgtacggggt cgacgcgggt ggtcagcgcc cagatcactt ccttccagtc gcgcacatcc
     7021 acatcgtcgt ccaccaccac aatgaacttg gtgtacatga actggcgcag gtggctccac
     7081 acccccatca tcacgcgccg cgcatggcca gggtaggcct tcttgatctg caccagcgcc
     7141 atgcggtaac tgcagccttc gggcggcaaa tagaaatcgg tgatctcggg aaactgccgt
     7201 tgcagtaacg gaatgaacag ctcgttgaga gccatgccca gcaccgcagg ctcatcgggc
     7261 ggcttgccgg tgtaggtgga gtggtagatc ggatcacgcc gcaggctgat gcggtccacc
     7321 gtgaacaccg gaaactcggc ctgctcgttg taatagccgg tgtggtcccc atacggtcct
     7381 tccagggcgt gctggtagcc gctggcgtgg ttggcatccg ggtagatgtg gccctccagc
     7441 acgatttcgg cactggctgg tacctgcagg ctgctgccca gtgcccgaac cacttcggtc
     7501 cgtgcgccgc gcaacagccc ggcaaactgg tattccgaca ggctgtcggg caccggggtg
     7561 acggccccca gaatggtggc cgggtccgcc cccagcgcga cggcaaccgg gtagggctgg
     7621 ccaggatgca gttggccgtg gtcccgaaaa tcaagcgcac ccccgcggtg ggccagccag
     7681 cgcatgatga gctggttgcg cgagagtacc tgctggcggt agatgccgag gttctgccgc
     7741 gctttgtgcg gaccgcgggt gatgaccagg ccccaggtga tgagcggcgc cacatcgccc
     7801 ggccagcagt gctggatggg taggcgggcc aggtccgcat ctggcccttc ccacaccacc
     7861 tcctggcagg gtgccgaact gcgttccttg ggcgccatgt tccacagggt tttgagcagg
     7921 ccgcgcatgc caaccatgtc cttgaagccc ttgggtgcct cgggctcctt gagggatgcc
     7981 agcagctcac cgaacgggcg caggccgctg atgctgttga cgcccatggc gcgcgctaca
     8041 cgctccggag tgccaaacag gttgcccagc actggcatgg tgtggccggt cggatgttca
     8101 aacaggagcg cggggccagc cgcgcgcaac acgcggtcgc acagggctgt catctccaga
     8161 tggggggaca ccggctgggt gacgcggcgc aattcgccgg cctgctccag ctgggccatg
     8221 aagtcgcgca ggtcgtgata agccatcttt gtaagttagg ggttggccgg ggtttgtccc
     8281 tgccagcgcg gtgccagggt gtggggcaga tcgagcagat ccagcacgcg ggcctgcgtg
     8341 gagaggtcga gggcgctcac gggctcatcg cagacgatca accgcggctc gagcgcgagg
     8401 gcgcgcgcga tggcgatgcg ctgacgctgc ccgccggaga actcgctggg gtagcggtgc
     8461 gaggcatccg tgggcagacc gactcggtcg agcaggtcct tgacccgcgc ccgagcct
//
LOCUS       DAOSJM010000053         8423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7878_length_8423_cov_3.931166, whole genome shotgun sequence.
ACCESSION   DAOSJM010000053 DAOSJM010000000
VERSION     DAOSJM010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8423
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7878_length_8423_cov_3.931166"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PKX82_03050"
     CDS             complement(<1..194)
                     /locus_tag="PKX82_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HOF51255.1"
                     /translation="MSAVLSAVDDYGQDRQIAQLRVPPHSIEGESSVLGGLLLDNDAW
                     DRVGDILQESDFYRYEHNLI"
     gene            complement(399..851)
                     /gene="rplI"
                     /locus_tag="PKX82_03055"
     CDS             complement(399..851)
                     /gene="rplI"
                     /locus_tag="PKX82_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOF51256.1"
                     /translation="MQIILLDKVVNLGNLGEIVKVKDGYARNFLIPSGRARRATETAK
                     AEFEARRAELEKIAAAKLAECQALGEKLGGTTIKLTQKAGVDGRLFGSVTNHDIAEEL
                     VKAGYKVVKSQIRMPNGPIKIVSDSTVSVALHTDVVVDITVSVYGETA"
     gene            complement(864..1145)
                     /gene="rpsR"
                     /locus_tag="PKX82_03060"
     CDS             complement(864..1145)
                     /gene="rpsR"
                     /locus_tag="PKX82_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOF51257.1"
                     /translation="MPGPKRFNKDKRPKRNTQSLLFKRKRFCRFTVTGVEEIDYKDID
                     TLRDFIAENGKIIPARLTGTRAIFQRQLNTAIKRARFLAMLPYSDQHKI"
     gene            complement(1162..1455)
                     /gene="priB"
                     /locus_tag="PKX82_03065"
     CDS             complement(1162..1455)
                     /gene="priB"
                     /locus_tag="PKX82_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HOF51258.1"
                     /translation="MPTNTLQLTACIAEAEAMRYTPAGLPALNLRLEHESEVLEAGGK
                     RVVKVVVKAVAFGTLAERLAKQALGSVWRFNGFLANARRGKTVVFHIQEFLQD"
     gene            complement(1478..1843)
                     /gene="rpsF"
                     /locus_tag="PKX82_03070"
     CDS             complement(1478..1843)
                     /gene="rpsF"
                     /locus_tag="PKX82_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOF51259.1"
                     /translation="MRHYEIILMIHPDQSEQVPAMLERYKGMITAGGGKVHRVEDWGR
                     RQMVYMINKLAKAHYLCVNIEADQAVMSELEHAFKFNDAVLRHLTVLKKKAETGPSSM
                     MKTVEREETRKAQQAEYQA"
     gene            complement(1970..4267)
                     /locus_tag="PKX82_03075"
     CDS             complement(1970..4267)
                     /locus_tag="PKX82_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VC_2705 family sodium/solute symporter"
                     /protein_id="HOF51260.1"
                     /translation="MIEAATRRQWSLHAGLLALWFVASFGVVFFARDLQVLVAGWPIG
                     FWFAAQGSVLVFIFVVVYFAWAKNRREEPEPGFNYSGFSARKRRLHWRFAAYTACLLL
                     FLVVLAVAERYGLPKAWVAGLFLSATLILYAVIGVYGRTADASEYYVAARRVPAVYNG
                     MATAADWMSAASFISMAGGLYLQGFSGSGGQPGGLAYILGWTGGFCLVAMLVAPYLRR
                     MNLYTVPDYFAVRFGGNWTRMIAAVAAVLVSFTYVVAQIYGVGLITSRLTGVHFEIGI
                     LLGLGGVLVCSFLGGMRAVTWTQAVQYVVIILAFLIPVSWLSYKQLGNPLAPIAYGQQ
                     LQKIAVMEDALMHSPVERQVIEEYARRADALDKKLLDVDATLQTERRELRAKLAALGG
                     GEAESDAANALRRELAAVPKDANSAREKWTRAAADYRERARPLGGMPAHDKPFAGDPD
                     GSPSEQKEFQSSRANFLALMFCLMVGTTGLPHLLTRFYTTPTVAEARRSVAWSLVFIG
                     LLYVSAPALAVLVKYEVMAHLVGQPYDSLPHWMAQWARDPTLLSFTDINGDHILQFAE
                     LKMGPDLVMLASPEIGGLPYVISVLVAAGGLAAALSTADGLLLTIGNALAHDAYFEGK
                     SSKAQAMRRVMWSKFALLVVALVAAYAAAQRPAGILYLVSASFSLAGAAFVPAMVLGI
                     FWRRTTRAGAVAGMLAGLGVTIYYMVANQAAVRQWLGLSGDGLWWDIQPVSGGVFGVT
                     CGLFATVLVSLLTTPEPPAGHRDDA"
     gene            complement(4264..6663)
                     /gene="ppsA"
                     /locus_tag="PKX82_03080"
     CDS             complement(4264..6663)
                     /gene="ppsA"
                     /locus_tag="PKX82_03080"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOF51261.1"
                     /translation="MSSQLFSPTDLVVPFEKLRMTDVDTVGGKNASLGEMISHLPTGV
                     KVPTGFATTAHAFREFLKFGGLTDKINARLSALDTEDVRALAQVGAEIRAMVEVQPFP
                     PELEKGIREAFEVLSAGNPEATFAVRSSATAEDLPDASFAGQQETFLNVHGIDDILHK
                     IREVFASLYNDRAISYRVHKGFAHEHVALSAGIQRMVRSDIGASGVMFTIDTESGFSD
                     VVFITSSYGLGETVVQGAVNPDEFYVHKPMLKAGNRALIRRSLGSKLIQMVFTSAEEK
                     VAAGGKLIKTTDVPTEMRNRYSLTDADVEQLARYAVVIEEHYGRPMDIEWGKDVVDGE
                     LYNQQARPETVKSQAGNSVEHRYKLVGKGTVLVEGRAIGQKIGTGPVRIVHNISEMDK
                     VQAGDILVTDMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGHATEA
                     LKDGMLVTVSCAEGDTGFIYDGLLETEVSEVQRGAMPEIPVKIMMNVGNPQLAFDFCQ
                     LPNQGVGLARLEFIINNNIGVHPKAILDYPNVDADLKKAVESVARGHASPRAFYVDRV
                     AEGVATIAAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGAARYVSAE
                     FGEAFAMECEALKRVRNDMGLNNVQVMVPFVRTLGQARKVTELLAKHGLRRGENELKL
                     VMMCEIPSNAILADQFLEYFDGFSIGSNDLTQLTLGLDRDSGLELLAADFDERDPAVK
                     ALISMAISACKRQGKYIGICGQGPSDHPDFAQWLEEQGISSISLNPDSVIATWQQLAV
                     K"
     gene            6811..7632
                     /locus_tag="PKX82_03085"
     CDS             6811..7632
                     /locus_tag="PKX82_03085"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOF51262.1"
                     /translation="MPQRTVFFVSDGTGITAETFGNAILAQFEAKTRMVRLPFVDTVD
                     KAHQVVRQINHAAELDGSRPIVFTTLVNMEVLEVIQGSCNGMLLDMFGMFVHPLEQEL
                     QIKSNHRIGRFSDASKSKEYQNRIEAINFSLAHDDGQSNRDLEQSDVILVGVSRSGKT
                     PTSLYLAMQYGLKASNYPLIPEDFDRRQLPPALVPHKAKIFGLTIQPERLSEIRNERR
                     PHSKYASLENCRNEVAEAEAMMRRTGIRWLSTTTKSIEEIATTILQEIHPGRLEY"
     gene            complement(7719..>8423)
                     /locus_tag="PKX82_03090"
     CDS             complement(7719..>8423)
                     /locus_tag="PKX82_03090"
                     /inference="COORDINATES: protein motif:HMM:NF019077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HOF51263.1"
                     /translation="SVRDPVTLRKVAQWPTLGIGPHQLLLDASGALMVANGGLLRAEG
                     DKKRDLHLMDSSLVRLDPKTGQRLGQWRLKDSRLGIRHMAWSQPQGAATGLAGTALLG
                     IALQNEHDDVNQRRNSPVLAVWDGETLRTPSLSPAGGGYAGDIVAGPDGGFVLSCLRV
                     NAALKWSPAEPEAMVNVALLRDVGALAPWGLSQESTGVLLGSARGVGRWHPRMAPVFL
                     AWPGAMALDNHWIIVG"
BASE COUNT         1800 a         2685 c         2344 g         1594 t
ORIGIN      
        1 taaatgagat tgtgctcgta ccggtaaaaa tcgctttctt gcaggatgtc cccgacccgg
       61 tcccacgcat cgttgtccag cagcaagcca ccgagcacgc tggactcacc ctcaatcgag
      121 tgcggaggaa cgcgcaactg cgcgatctga cggtcttggc cgtagtcgtc tacggcggaa
      181 agtacggctg acatggtgaa ttccttagtg cccctgatgc tacgcggagc accgcatcac
      241 gtcgagggaa aagcctgggc acaaccgggg aacaagctgt gcgtaaggtg tgcaaatccg
      301 gggataacct gagacaacaa agacaaaagc cgcaagggag caaaccctgg cggctttttg
      361 gtatgccccc gcacccgaag gcacggggtc aatggcgttt aggcagtttc gccgtacacg
      421 gacacggtga tgtcgaccac tacgtcagtg tgcagagcca cactcacggt gctgtcgcta
      481 acgatcttga taggaccgtt gggcatgcgg atttgtgact tgacgacctt gtaaccggcc
      541 ttgaccagct cttcagcgat gtcatggttg gtcacggaac caaacaggcg tccgtccaca
      601 ccggcctttt gggtcagctt gatggtggtg ccaccgagct tttcacccag agcctggcac
      661 tcagccagct tggctgcagc gattttttcc agttcggcgc ggcgggcttc gaattctgcc
      721 ttggcagttt cggtagcgcg gcgggcacgg ccagaaggaa tcaggaagtt gcgggcatag
      781 ccatctttga ccttgacgat ttcgccgagg ttgccgaggt tcacgacctt gtcgagcaga
      841 atgatttgca tggtcggggc tccttagatc ttgtgctggt cgctgtaagg cagcatcgcc
      901 agaaagcgag cgcgcttgat agcggtgttc agttggcgct ggaaaatggc gcgggtgccg
      961 gtcaggcgtg cgggaatgat cttgccgttt tcagcaatga agtcacgcag tgtgtcgatg
     1021 tctttgtagt caatctcttc cacaccggtc acggtgaagc ggcagaagcg cttgcgcttg
     1081 aacaacaggg attgcgtatt gcgcttgggg cgcttgtctt tgttgaaacg ttttggtccg
     1141 ggcatgatgg actcctaaaa attaatcttg caaaaattct tgaatgtgaa aaacaactgt
     1201 ctttccccgg cgtgcattgg caagaaaacc gttgaacctc cagacactgc ctaacgcctg
     1261 ctttgcaagc cgctctgcca gtgttccgaa agctaccgct ttcaccacca ccttcactac
     1321 acgcttgcca cctgcctcca gaacttcgga ctcgtgttcg agtcgcagat tcagggctgg
     1381 taaacctgcc ggtgtgtagc gcatggcctc tgcctctgcg atacaagccg tcaattgcaa
     1441 ggtgttggtg ggcactactc caggaatgga cggttattta cgcctggtac tcagcttgct
     1501 gagccttgcg tgtttcttca cgctcgacgg tcttcatcat ggaggaagga ccggtttcgg
     1561 ccttcttctt caagacagtc aggtggcgca gcacagcgtc gttgaacttg aacgcatgtt
     1621 ccagttcgga catcacggcc tggtcggctt cgatgttgac gcacaggtag tgggccttgg
     1681 ccagcttgtt gatcatgtag accatctgac gacggcccca gtcttcaaca cggtgcacct
     1741 tgccaccgcc agcggtgatc atgcccttgt agcgttccag catggccgga acctgctcgc
     1801 tctgatccgg gtggatcatg aggatgattt cgtaatgacg cattgatact ccttgtggat
     1861 gtcccagcat ctgggtcccc ggcactcgcg tgccggtcta aataaagctg cccccggcgt
     1921 ctaaacaggg tgcagcaagg cgaagcccat gattatagcg caagccgtgt caggcgtcat
     1981 ccctgtgccc agcaggtggc tccggggtag tcaaaaggct caccagcacc gtcgcaaaca
     2041 gtccgcacgt caccccgaac acaccacccg ataccggctg gatatcccac cacaagccgt
     2101 cgccagacag tcccagccat tggcgcactg cggcctgatt ggccaccatg tagtagatcg
     2161 tgacgcccag tcctgccagc attccggcga ccgcacccgc gcgtgtcgtg cgccgccaga
     2221 aaatacccag caccatcgca ggtacaaaag cggcgccagc caaagaaaac gaggcggaaa
     2281 ccagataaag gatgccggcc ggccgctgcg ccgcagcata ggcagccacc agggccacca
     2341 ccagcaatgc aaacttggac cacatcaccc ggcgcatggc ctgtgccttg ctgcttttcc
     2401 cctcaaaata cgcatcgtgc gccagcgcat tgccaatggt cagcaacaaa ccgtcggccg
     2461 tggacaaggc agcggccagg ccacccgctg cgaccagaac agaaataaca tagggaagcc
     2521 ccccgatctc cggcgatgcc agcatgacca ggtccggtcc catcttcaac tcggcgaact
     2581 ggagaatatg gtcgccgttg atatccgtga aagacagcaa ggtcgggtcg cgcgcccact
     2641 gcgccatcca gtgcggcaaa ctgtcatacg gctgccctac cagatgcgcc atgacctcgt
     2701 acttgaccag gaccgccagt gcgggtgccg agacatacag caagccaata aagaccagag
     2761 accacgccac cgaacgccgc gcttcggcca ctgtcggcgt cgtgtaaaaa cgggtcagca
     2821 ggtgcggcag cccggtggtc ccgaccatca ggcaaaacat cagcgccagg aagttggcgc
     2881 gggaactctg aaattccttc tgctcactgg gactgccatc cgggtcacct gcaaagggtt
     2941 tgtcatgggc aggcatgccc cccagcggcc tcgcacgctc ccggtagtcc gccgccgcac
     3001 gggtccattt ttcacgggcc gagttggcgt ctttcggaac ggcagccaat tcacggcgca
     3061 aagcgttggc ggcatcggac tctgcttccc ccccccccaa cgcggccagc ttcgcgcgca
     3121 actcccggcg ttcggtctgc aacgtcgcat cgacgtccag caactttttg tccagcgcat
     3181 cagccctgcg cgcgtattct tcaatcacct gccgctcgac gggcgaatgc atcagtgcat
     3241 cttccatcac cgcaattttt tgcaactgct gaccataggc aatgggagcc agcggattgc
     3301 ccagctgttt gtaggacagc caggacaccg ggatcaggaa cgccagaata atgaccacgt
     3361 actggactgc ctgcgtccag gtcaccgccc gcattccgcc cagaaacgaa cacaccagaa
     3421 caccaccgag cccgagaagg attccgattt caaaatgcac accagtcagt cgggatgtga
     3481 tcaggcctac tccataaatc tgcgctacca cataggtgaa agagaccagc acagccgcca
     3541 cggcagcaat catgcgtgtc caattgcccc caaagcgcac cgcaaaataa tcgggcaccg
     3601 tgtagaggtt catgcggcgc aggtagggcg caaccagcat cgcaaccaga cagaagccac
     3661 cggtccatcc caggatgtaa gccaggcctc cgggctgacc gcctgaaccc gaaaaaccct
     3721 gcagatacag tccacccgcc atgctgatga acgaggcagc actcatccag tcggccgcag
     3781 tcgccatgcc gttgtacacg gctggcaccc gccgcgcggc cacatagtat tccgacgcat
     3841 cggccgtgcg accatagacc ccgatgacgg catacagaat cagcgttgcc gacagaaaca
     3901 ggcccgccac ccaggctttg ggcaagccat agcgctcagc tacggcaagc accaccagga
     3961 acagcagcag acaagcggtg taggccgcaa aacgccagtg caaacgccgc ttgcgggccg
     4021 aaaaaccgga atagttaaaa ccaggctcgg gctcctcacg gcggttcttc gcccatgcga
     4081 aatacaccac cacaaagata aagaccagta ccgagccttg cgccgcaaac cagaacccga
     4141 tgggccatcc ggccaccaga acctggaggt cgcgtgcaaa gaagacgact ccaaaagaag
     4201 caacaaacca caacgccagc agccctgcat gcagagacca ctggcgccgt gtagctgcct
     4261 ctatcattta acggcgagtt gctgccaggt tgcgatgacg gagtcgggat tgagcgaaat
     4321 ggacgaaatc ccctgctctt ccagccactg tgcaaagtca ggatggtcgc tgggaccctg
     4381 gccgcagata ccgatgtatt tgccctggcg cttgcaggcg gaaatagcca tgctgatcag
     4441 tgccttgacg gcagggtccc gctcgtcaaa gtctgccgcc agcaactcca gaccggaatc
     4501 gcggtccagc cccagcgtca actgcgtcag gtcgttggag ccgatggaga acccgtcgaa
     4561 atactccagg aactggtctg ccaggatggc gttgctgggg atctcgcaca tcatcaccag
     4621 cttgagttcg ttttccccac ggcgcaggcc gtgtttcgcc agcagctccg tgactttcct
     4681 ggcctggccc agcgtacgga cgaagggaac catgacctgc acattgttca ggcccatgtc
     4741 gttgcgcacc cgcttgagcg cttcgcactc catggcaaag gcctcgccga actcggctga
     4801 cacataacgt gcagctcccc ggaagcccag catggggttc tcttcctcgg gctcgtagcg
     4861 gctgccacca atgagcttgc ggtattcgtt gctcttgaag tccgacaggc gcacgatcac
     4921 tggcttgggc cagaaagccg ctgcaatggt ggcaacgcct tcagcaaccc ggtccacata
     4981 aaacgcacgg ggtgacgcat ggccgcgggc taccgactcg actgcctttt tcaggtcggc
     5041 gtcgacgttg gggtagtcca gaatcgcctt gggatgcaca ccaatgttgt tgttgatgat
     5101 gaactccagc cgggccaggc ccacgccctg gttgggcaac tggcagaagt caaacgccag
     5161 ctggggattg cccacgttca tcatgatctt gacgggaatc tcgggcatgg caccgcgctg
     5221 cacttcggag acttcggtct ccagcaggcc gtcgtaaata aagccggtat cgccttccgc
     5281 gcagctcacc gtcaccagca tgccgtcctt gagcgcctct gtagcgtggc cacagcccac
     5341 caccgccgga atacccagtt cgcgcgcgat gatggccgca tggcaggtgc gtccaccgcg
     5401 gttggtcacg atggcagacg cccgcttcat gaccggctcc cagttggggt cggtcatgtc
     5461 agtcaccaga atatcaccgg cctgcacctt gtccatctcg ctgatgttgt gcacgatgcg
     5521 caccggtccg gtaccgatct tctgaccgat cgcacgccct tccaccagca ccgtgccttt
     5581 gcccaccagc ttgtagcggt gttcgaccga attgccggcc tggctcttga ccgtctcggg
     5641 tcgggcctgc tggttgtaca gctcgccatc cacaacgtct ttgccccact cgatgtccat
     5701 ggggcgtccg taatgctctt cgatcaccac ggcgtagcgt gccagttgtt ccacatccgc
     5761 gtccgtcagg gagtagcggt tgcgcatttc cgtgggcaca tcggtggtct tgatcagctt
     5821 gccgccggcc gcgacttttt cctcggcact ggtaaacacc atctggatca gcttggaacc
     5881 caggctgcgg cggatcagcg cacgattgcc tgctttgagc atgggcttgt gcacatagaa
     5941 ctcatcaggg ttcaccgcgc cctgcaccac cgtctcaccc aggccatagc tggaggtgat
     6001 gaacaccacg tccgagaagc ccgactcggt atcaatggtg aacatgacac cggatgcgcc
     6061 aatatccgag cgcaccatac gctggatgcc ggcgctcagc gccacgtgct cgtgggcaaa
     6121 gcccttgtgc acccgatagc tgatggcgcg gtcgttgtac agggacgcaa agacctcgcg
     6181 aatcttgtgc aggatatcgt cgataccgtg cacgttcagg aaggtttcct gttgccctgc
     6241 aaatgatgca tcaggcaggt cttcggcggt agccgaggaa cgcacggcaa aggtcgcttc
     6301 agggttaccc gcactcagca cctcaaacgc ttcgcggatg cctttttcca gttctggagg
     6361 aaaaggctgg acttccacca tggcacggat ttcggcaccc acctgggcca gggcgcgcac
     6421 gtcttcggta tcgagcgcgc tcagtcgtgc gttgatcttg tcggtcaggc cgccgaactt
     6481 gagaaattcg cggaaggcat gcgccgtcgt ggcaaagccg gtaggaacct tcacccccgt
     6541 aggcaggtgg gagatcattt cccccaagct ggcgttttta ccgccgaccg tgtccacatc
     6601 ggtcatccgc agtttttcga atggcacaac cagatcggtt ggactaaaca gttgagaaga
     6661 cattgcaata ctccagagtt taaaaaccgg cgcgcgcatc gctcgaaatg cgctaaatta
     6721 cgaacattgt aggggcgcca ccacggcggc gcatactcca tttcccgtcc gcgcttgcag
     6781 gacactgacg accccaacga cttcaccagc atgccccaac gcaccgtatt ttttgtgtct
     6841 gacggcaccg gcatcaccgc cgagaccttt ggcaacgcca tcctggccca gtttgaagcg
     6901 aaaacccgca tggtgcgcct gcccttcgtg gacaccgtgg acaaggccca ccaggtagtc
     6961 cggcagatca accacgctgc cgagctggac ggaagccgcc ccattgtgtt taccaccctg
     7021 gtcaacatgg aagtgctcga agtcatacag gggagctgca acggcatgct gctggacatg
     7081 tttggcatgt tcgtgcaccc cctggaacag gaactgcaga tcaagtccaa ccaccgcatt
     7141 ggccgattca gtgacgccag caagagtaag gaataccaga accgtatcga ggccatcaat
     7201 ttttcgctgg cccacgacga cggccagtcc aatcgggatc tggagcagtc cgacgtcatc
     7261 ctggtgggcg tcagccgcag cggcaagaca cccacctcgc tgtacctggc catgcagtac
     7321 ggcctgaagg cgtctaacta ccccctgatt cctgaagact ttgaccgccg ccagttgccc
     7381 cccgcactgg tgccccacaa ggccaagatt ttcggtctga ccatccagcc cgagcgcctc
     7441 tccgaaatcc gcaacgaacg gcgcccccat tccaaatacg catcgctgga aaactgccgc
     7501 aatgaagtgg ccgaagccga agccatgatg cgccgcaccg gcatccgctg gctttccacc
     7561 acgaccaagt ccatcgaaga gatcgccacc accatcctgc aggaaattca cccgggacgg
     7621 ctggagtact aattagctat taatttgata gctatctacg cagcatccac gggggccaga
     7681 ggctaatttc ttcatttatt tcgtcgggag cggcccggtt aaccgacgat gatccagtgg
     7741 ttgtccagcg ccatggcacc cggccacgcc agaaaaacgg gcgccatgcg cgggtgccag
     7801 cgccccacgc cgcgggccga gcccagcaat acaccggtcg actcctgcga cagcccccac
     7861 ggcgccaaag cgcccacatc gcgcagcagt gccacgttga ccatggcttc gggttcagca
     7921 gggctccact tcaaggccgc attcacgcgc agacagctca gcacaaatcc gccatcggga
     7981 cctgccacga tgtcgcctgc gtagccgccg cccgccggtg acagggaggg cgtccgcaat
     8041 gtctcgccat cccacaccgc caatacaggc gagttgcggc gctggttgac atcgtcatgc
     8101 tcgttttgca gggcgatgcc cagcagtgcc gttccggcca ggcccgtggc cgcaccctgc
     8161 ggctggctcc aggccatgtg gcgaataccc agacgggaat ccttcagccg ccattgcccc
     8221 aggcgctggc cggttttcgg gtccagccgg accagcgagg aatccatcaa gtgcagatcc
     8281 cgcttcttgt cgccctcggc gcgcagcagc ccgccattgg ccaccatcaa agcacccgaa
     8341 gcatccagca gcaactggtg cggcccaata cccaaagtgg gccattgggc caccttgcgc
     8401 aaagtcaccg ggtcccgcac gga
//
LOCUS       DAOSJM010000054         8415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_7888_length_8415_cov_4.131459, whole genome shotgun sequence.
ACCESSION   DAOSJM010000054 DAOSJM010000000
VERSION     DAOSJM010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7888_length_8415_cov_4.131459"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="PKX82_03095"
     CDS             <1..922
                     /locus_tag="PKX82_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOF51264.1"
                     /translation="SLGAANKSTNRKDLRNVGPSVSYKLRDAAGQAREFNNYMLPVDM
                     GEGVPVFLLGMRESPSEPLRYLRVPADASGSMDDFMRMRYALLDPVLRDKAVKRYAAK
                     AVDARNPELATQLGASAARALSLFAGENSVRPAAGLQTVADFIEANVPEAERNKAGEV
                     LIRILNGTVYELAQITREQAGLPPLVADERTQMFMTQAVLALSDAPLYPAPVALQLTD
                     FTHVQASVFQVARAPGKMVVYLGCALLILGVFAMLYIRERRLWIWFSPVAGADADVAH
                     ATMALSTNRKTMDGDREFDQLKARLLGVTS"
     gene            942..2285
                     /gene="ccsB"
                     /locus_tag="PKX82_03100"
     CDS             942..2285
                     /gene="ccsB"
                     /locus_tag="PKX82_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HOF51265.1"
                     /translation="MPTGSSQGGSTGQVEILHSGYFARRNAFDWVLALAVICGCVFAF
                     MQYHAAMDVYEKGILIAAVPCAIMLAWFWRPLGILMLVVAGLSLAGIVSYQGNLARAE
                     TVFWLKYFLSSQSAILWMSMLFFMSTVFYWIGVFAKGRAEGMELVASRLVWVAVTMAL
                     TGTMVRWYESYLLGADIGHIPVSNLYEVFVMFCWMTATFYLYFEAQYKTRALGAFVML
                     VVSAAVGFLLWYTVVRQAHEIQPLVPALKSWWMKLHVPANFIGYGTFALSAMVAFAYL
                     IKQQATETRWYKLAPLWLLGVVLCFEPIVFRKSAVAGMSDYWAVYFGISALIVASIVF
                     GRQRIAARLPSFEILDDVMYKAIAVGFAFFTIATVLGALWAAEAWGGYWSWDPKETWA
                     LIVWLNYAAWLHMRLMKGLRGTVAAWWALAGLAVTTFAFIGVNMFLSGLHSYGTL"
     gene            2342..3349
                     /gene="msrP"
                     /locus_tag="PKX82_03105"
     CDS             2342..3349
                     /gene="msrP"
                     /locus_tag="PKX82_03105"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HOF51266.1"
                     /translation="MLIKTNSHGFNHPIASEITDRTVYEQRRSMLKALAAGAAGSSMA
                     VWAAREALAQPATWGTRPGKLAALAGGVTRADGGMTLDKVTPYKDASTYNNFYEFGTD
                     KSDPAVNAHTLKTRPWSVEIEGLVKKPGKLALEDLLRLSPMEERIYRLRCVEGWSMVI
                     PWVGYALADLIKRVEPLGSAKYVEFVTLADPKTMPFVGSRVLDWPYVEGLRLDEAMHL
                     LTLLTFGMYGEVLPNQSGAPVRLVVPWKYGFKSGKSIVKIRFTDKQPGTAWNKAAANE
                     YGFYSNVNPQVDHPRWSQATERRIGEDGLFAKKRKTLMFNGYEAQVGQLYAGMDLKKF
                     Y"
     gene            3368..3973
                     /locus_tag="PKX82_03110"
     CDS             3368..3973
                     /locus_tag="PKX82_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HOF51267.1"
                     /translation="MNTWLLHKAAKPAAFLLGLLPALWLVYGAAFDQLGANPAEALVR
                     STGDWTLRMLCAVLAVTPLRVSAGLPGLARFRRMVGLFVYFYATLHLLAYSWFDMGLD
                     AGDIARDIAKRLFILVGFTAVLLLTPLALTSSNWMIRWLGARRWQALHRSVYVVAALA
                     VLHFFWMRAGKNNFTEVAVYAAVLATLLGWRAQRHFNKKRP"
     gene            complement(4016..5305)
                     /gene="lysA"
                     /locus_tag="PKX82_03115"
     CDS             complement(4016..5305)
                     /gene="lysA"
                     /locus_tag="PKX82_03115"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOF51268.1"
                     /translation="MTSPSIPGQPHIHYADGQLQVEGCSLDGLATEFGTPLFVYSKAS
                     MLAALAAYQRGFSGRNAQICYAMKANSSLGVLQVFAQAGCGFDIVSAGELARVITAGA
                     DASKVIFSGVGKTRTEMRQALEAGIGCFNVESEAEIDVLNAVAVSMGKCAPISIRVNP
                     NVDPKTHPYISTGLKGNKFGIAHERTLATYQHAASLPGLQIVGIDCHIGSQITEETPY
                     LDAMDRMLDLVESIEAAGIPIHHIDFGGGLGIDYQGDTPPAADALWAKLLTKLDARGF
                     GQRKLMIEPGRSLVGNAGVCVTEVLFMKPGEQKNFCIVDAAMNDLPRPAMYQAFHTIV
                     PLDEALHIKGKTDGLVCDVVGPVCESGDWIGRDRALAVQAGDRLAVLSAGAYCMSMSS
                     NYNSRPKAAEILVDGQNHHLIRVRDTMADQMRGEKLL"
     gene            complement(5311..5478)
                     /locus_tag="PKX82_03120"
     CDS             complement(5311..5478)
                     /locus_tag="PKX82_03120"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HOF51269.1"
                     /translation="MLKAKQILVSAIVLGAVVLNLAGCGQTGALYLPERASPKPFAPA
                     TPVVTPESKKP"
     gene            5491..5820
                     /gene="cyaY"
                     /locus_tag="PKX82_03125"
     CDS             5491..5820
                     /gene="cyaY"
                     /locus_tag="PKX82_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HOF51270.1"
                     /translation="MTDPEFLDYAEALLKAVEESCDRLNDATDADVDNQRVGSMVTLT
                     FANRSQIIINLQKPLHEVWMATKSGGYHYRWEKGQWQDTKGQGEFFDALSRNATAQAG
                     AALKFSS"
     gene            complement(5821..8187)
                     /locus_tag="PKX82_03130"
     CDS             complement(5821..8187)
                     /locus_tag="PKX82_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HOF51271.1"
                     /translation="MATPSNKSDSPKAGNGSQHHWLVRVVLWIGGLAMAGALSLLLVI
                     ALALSVAFPNLPDISDLSDYRPKLPLRVFSAEGDLLGEFGEERRNLTPIKEIPPVMAN
                     AVLAIEDARFYQHGGVDYIGVIRAGLANVGRVKSQGASTITMQVARNVYLTSEKTFTR
                     KIYEILLTFKLEHLLTKDQILEIYMNQIFLGHRAYGFAAASEAYFGKPLKDITIAEAA
                     MLAGLPKAPSAYNPITNPKRARSRQLYIIERMLENGFITAVEAETAKKQELKIRTGAN
                     TKQVHAEYATEMVRQLMYAQYGDETYTRGLNVYTTLRTADQDAAYRALRRGIMDFERR
                     QIYRGPEKFITLPSTAQEIEDAIDDALDEHPDNGDVMSAVVLEADAKHIKAIRQSGET
                     LEITGEGLKPAQSGLSDKAPPNIKVRRGAIIRVAKTAKGAWEITQLPEVEGAFVSMDP
                     RDGSIKALVGGFDFAKNKFNHVTQAWRQPGSSFKPFIYSAALEKGFTPATVVNDAPLF
                     FDAGVTGGQPWEPKNYDGTFEGPMTLRRGLAKSKNMISIQILQSIGTQYAQDWITHFG
                     FEAERHPPYLTMALGAGSVTPMQMATAYSVFANGGYRVNPWLVSRVSEQRGKVLVDIP
                     PLAPDETQRTIEPRNAFLMNSLLQEVTRSGTAARAQATLKRPDIYGKTGTTNDSMDAW
                     FAGYHQTLAAITWIGYDTPRKLGDRETGGGLSLPIWISYMETALKGVPVTEPAVPEGI
                     LNIGGEWFYDEFAKGAGVSGLGLEDKPAAPQPLPAGDEKKKILDLFKD"
BASE COUNT         1635 a         2243 c         2609 g         1928 t
ORIGIN      
        1 cagcctgggg gcagctaaca agagcactaa tagaaaagac ctgcgcaatg tgggtcccag
       61 tgtcagctac aaactgcgtg atgctgcagg tcaggcccgt gaattcaaca actacatgct
      121 gcctgtggat atgggtgagg gcgtgccggt gttccttctg ggaatgcgtg aatcgccttc
      181 ggagcctttg aggtacctgc gcgtgcctgc agatgcaagc ggttccatgg atgattttat
      241 gcgtatgcgc tatgccctgc tggacccggt cctgcgggac aaggccgtaa agcgctacgc
      301 cgccaaggcg gtggatgcgc gcaatccgga gctggcaacg cagctgggtg cctctgctgc
      361 acgtgcgctg tccctgtttg cgggagagaa tagtgtgcgc cctgcagcgg gcctgcagac
      421 agtggcagat ttcattgaag ccaatgtccc ggaggcagag cgcaacaagg cgggcgaagt
      481 cctgattcgc atactcaatg gcaccgtgta cgagttggcc cagatcaccc gggagcaagc
      541 gggtttgccg cctctggtgg cggatgagcg aacccagatg ttcatgacgc aggcggtgct
      601 ggccctgagt gatgcgccac tgtatccagc gccggtggct ctgcaactga cggatttcac
      661 ccatgttcag gccagcgtct tccaggtggc ccgggcaccc gggaaaatgg tggtttacct
      721 tggctgtgcc cttctgatac tgggggtatt tgccatgctg tacatacggg agcggcgttt
      781 gtggatatgg ttcagccctg tagcgggcgc cgacgctgat gtagcccacg ccaccatggc
      841 tttgtcgacc aatcgcaaga ccatggatgg tgaccgtgaa tttgaccaac tcaaagcccg
      901 tctgctgggc gtgacttcgt aagcacatga caacactaca catgccaact ggctcttccc
      961 agggtggatc aacggggcag gttgaaatac tccattcagg ttactttgcg cgtagaaatg
     1021 cgtttgactg ggtgctcgct ctggctgtca tttgtggctg tgtgtttgca ttcatgcagt
     1081 accacgcagc catggatgtg tatgagaaag gcattctgat tgccgcagtt ccctgcgcca
     1141 tcatgctggc ctggttctgg cgcccgctgg gcatcctgat gctggtggtt gccggactgt
     1201 cactggctgg aatcgtgtct taccagggaa acctggcgcg tgccgagacg gtgttctggt
     1261 tgaagtactt cctgtctagc cagtctgcca tcctctggat gagcatgctc ttcttcatga
     1321 gcacggtgtt ctactggatt ggcgtgtttg ccaaaggccg tgccgagggg atggagctgg
     1381 tggcgtcgcg tctggtctgg gtggctgtca ccatggcttt gactggcacc atggtgcgct
     1441 ggtatgagag ttaccttctg ggcgccgaca tcgggcatat cccggtcagc aatctgtacg
     1501 aagtgttcgt catgttttgc tggatgacgg cgacgttcta tctttatttc gaagcccagt
     1561 acaagacacg tgctctgggg gcatttgtca tgctggtggt gagtgccgcg gtgggttttc
     1621 tgctctggta caccgtggtg cgccaggcgc acgagatcca gcctctggta cccgccctca
     1681 agagctggtg gatgaagctg catgtgccgg ccaacttcat tggctatggg acgtttgccc
     1741 tatccgccat ggtggctttt gcctacctga tcaagcagca ggcgaccgaa acgcgctggt
     1801 acaagctggc gccactgtgg ctgctgggcg tagtgctgtg ctttgaaccc atcgtgtttc
     1861 gcaagtcggc tgtcgcgggt atgagcgatt actgggccgt gtatttcggc atctcggccc
     1921 tcatcgtggc gagcattgtt tttggccgcc agcggattgc agcgaggtta ccctcgtttg
     1981 aaatccttga tgacgtgatg tacaaggcca ttgctgtggg ttttgctttt ttcacgattg
     2041 caacggtgct tggcgcgctg tgggccgcgg aggcttgggg cggctactgg agctgggacc
     2101 ccaaggaaac ctgggcgctg attgtctggc tcaactatgc agcgtggctg cacatgcgcc
     2161 tcatgaaggg cttgcgtgga accgtggcag catggtgggc acttgcggga ctggcggtca
     2221 ccactttcgc gttcatcggt gtcaacatgt tcctgagcgg tttgcacagc tacggaaccc
     2281 tgtagtgtgc gggtagagaa acgtcgctga cggacgacca accaatatcg aggacgtctt
     2341 tatgctgatc aagaccaatt cccacgggtt caaccacccc atcgccagcg agatcacgga
     2401 ccgtacggtg tatgagcagc ggcgcagcat gctcaaagcg ctggccgccg gcgctgccgg
     2461 aagttccatg gcggtatggg ctgcacgcga ggcactcgcg cagcctgcga cgtggggtac
     2521 gcgtccaggc aagctggctg cactggcagg cggcgtgacc cgggcggatg gcgggatgac
     2581 gctggacaag gtcactccgt acaaggacgc cagtacctac aacaatttct acgaattcgg
     2641 cacggacaag agcgatccgg cggtcaacgc ccataccctg aaaacgcgcc cttggtcggt
     2701 agaaattgaa ggcctggtca aaaagcccgg gaagctggcg ctggaagacc tgctcagact
     2761 cagccccatg gaagagcgta tttaccgtct gcggtgcgtc gagggctggt cgatggtgat
     2821 tccctgggtc ggttacgcac tggcggatct gatcaagcgt gtggagcccc tggggagtgc
     2881 gaagtacgtg gagtttgtga ctctggcgga tccgaagacc atgccttttg tggggtcgag
     2941 ggtgctcgac tggccgtatg tggagggctt gcgtctggat gaagccatgc atctgctgac
     3001 attgctgacg tttggcatgt acggtgaggt gctgcccaac cagagcggag ctccggtgcg
     3061 actggtggtt ccctggaagt acggcttcaa aagcggcaag agcattgtca agatccgctt
     3121 tacggacaaa caaccgggta cggcgtggaa caaggcggca gccaacgaat atggtttcta
     3181 ttccaacgtc aatccccaag tggaccaccc gcgctggagt caggcgaccg agcgccgcat
     3241 tggtgaggac ggattgtttg ccaagaagcg caaaaccctg atgttcaatg ggtatgaagc
     3301 acaggttggc cagttgtacg ctggcatgga cctcaagaag ttctactgat tgggcgtgag
     3361 gacatttttg aacacatggc ttctgcacaa ggctgccaaa ccggcagcct ttttactggg
     3421 gctgctccct gcactgtggc tggtttatgg cgcggcgttt gatcagttag gggccaatcc
     3481 ggcagaggcg ctggtgcgct caaccggcga ctggaccttg cgaatgctgt gtgctgttct
     3541 ggcggtgaca cctttgcgtg tcagcgcggg cttgccaggg ttggcccgct ttcggcgcat
     3601 ggtggggctg ttcgtgtact tctatgccac gctgcacctg ctggcctaca gctggtttga
     3661 catggggctg gatgccggtg acattgcccg tgacattgca aagcgtctgt tcattctggt
     3721 gggctttacc gccgtgctgc tgctgacgcc gctggcatta acttcatcga actggatgat
     3781 tcgatggctg ggggcgcggc gttggcaggc gttgcaccga agcgtatatg tggtggctgc
     3841 gctggccgtg ctgcattttt tctggatgcg cgccggcaag aacaacttca ccgaggtggc
     3901 ggtgtatgcc gctgtgctgg cgacgctctt gggatggcgc gcacagcggc attttaataa
     3961 aaaacgcccc tagccctcgt ggaatatgcg taagcagcta ttaaaagagt agcaatcaga
     4021 gcagcttctc gccccgcatc tggtctgcca tggtgtcgcg gacgcgaatc aggtggtggt
     4081 tctggccatc caccagaatc tcggcggcct tggggcggga gttgtagttg ctcgacatgc
     4141 tcatgcaata ggcacccgca gacaagaccg cgagccggtc accggcctgc actgccagag
     4201 ccctgtcgcg accgatccaa tcgccactct cgcagacggg gcccaccacg tcacatacca
     4261 gtccgtccgt cttgcccttg atgtgcagcg cttcatccaa cggaacgatg gtgtgaaaag
     4321 cctggtacat ggctgggcgc ggcaggtcgt tcatggcggc atccacaatg cagaaattct
     4381 tttgctcgcc aggtttcatg aacagtactt cggtaacgca aaccccggca ttgcctacca
     4441 gtgagcgacc cggttcgatc atcagcttgc gttggccaaa accacgtgcg tcgagcttgg
     4501 tgagcagctt ggcccacagc gcgtccgcgg caggaggtgt atcgccctgg tagtcgatgc
     4561 ccagaccgcc accaaaatcg atgtggtgga tggggatgcc tgcggcctcg atgctttcaa
     4621 ccaggtccag catgcggtcc atggcgtcca ggtagggcgt ttcttccgtg atctgggagc
     4681 caatatggca atcaataccg acgatctgca gtcccggcag agacgccgcg tgctggtagg
     4741 ttgccagcgt gcgctcgtgt gcgataccaa acttgttgcc cttgagcccg gtcgagatgt
     4801 aagggtgcgt cttggggtcc acattgggat tcacgcgaat gctgataggc gcacatttgc
     4861 ccatggatac agccacagcg ttgagcacat ctatctctgc ctcgctctcc acattgaagc
     4921 atccgatgcc ggcttccagc gcctggcgca tctcggtgcg ggtttttccg acaccggaaa
     4981 aaataacctt ggaggcatcg gcaccagcag tgatgacgcg ggccagctca ccggcagaga
     5041 caatgtcaaa gccgcatccg gcctgcgcga acacctgcag tacacccaag cttgagttcg
     5101 ccttcatggc gtagcaaatc tgggcgttcc tacccgaaaa accgcgctgg taggccgcca
     5161 atgcggccaa catggatgcc ttggaataca cgaacaacgg cgtgccaaat tcagtggcga
     5221 gtccatcaag ggagcagccc tcgacctgca actgtccgtc agcgtagtgg atgtggggtt
     5281 gtcctggaat ggaaggggag gtcattggat tcacggtttt ttagattcag gggtaaccac
     5341 tggggttgca ggagcgaatg gtttggggct ggcacgctct ggcagataca gtgccccggt
     5401 ctggccacag cccgccagat tcagcacaac tgcaccaagg acaatcgcac tgactagaat
     5461 ttgctttgct ttcaacatgg tgaaattgta atgaccgacc ccgaattcct ggattacgcc
     5521 gaagccctgc tcaaagcggt ggaagaaagc tgtgaccgac tcaacgatgc aacggatgcc
     5581 gatgtggaca atcaacgcgt gggcagcatg gtcacgctga cattcgccaa ccgcagccag
     5641 atcatcatca atctgcaaaa gccattgcac gaagtgtgga tggcgaccaa gtcggggggc
     5701 taccactacc gttgggaaaa ggggcagtgg caggacacca agggacaagg tgaattcttt
     5761 gacgcactgt cccgcaacgc gacggcacag gctggggcag cactcaaatt ctcatcctga
     5821 tcaatctttg aataggtcca ggattttttt cttttcatcg cctgccggaa ggggttgtgg
     5881 tgcggcaggt ttgtcctcaa ggcccaaacc cgatacgcca gcccctttgg cgaattcgtc
     5941 gtagaaccat tcgccaccga tgttgaggat gccttcgggc accgcgggct ccgttaccgg
     6001 aacgcccttc aaggcggttt ccatgtagct gatccaaatg ggtaagctca atcccccacc
     6061 cgtttcgcga tcgcccagtt tgcgcggcgt gtcatagcca atccaggtga tggccgccag
     6121 cgtctggtgg tagcctgcaa accaggcgtc catggagtca ttggtggtgc cggttttgcc
     6181 gtagatatcc ggcctcttga gcgttgcctg ggcccgtgct gccgtgcctg agcgggtgac
     6241 ttcctgcagc agactgttca tgaggaatgc attgcgaggt tcgatggtgc gctgggtctc
     6301 gtcgggcgcc aggggaggaa tatctacgag cactttgccg cgctgctcac tgacgcggct
     6361 gaccagccat gggttgacgc ggtagcctcc gttggcaaat accgaatatg ccgtagccat
     6421 ttgcatgggg gtgaccgagc ccgcgcctaa agccatggtg aggtagggtg ggtggcgctc
     6481 ggcctcaaaa ccgaaatggg tgatccagtc ctgcgcgtac tgggttccaa tggattgcag
     6541 tatctggatg gagatcatgt tcttggactt ggccagaccg cggcgcagtg tcatagggcc
     6601 ttcgaaggta ccgtcgtagt tcttgggttc ccaaggttgt cctccagtga cccctgcatc
     6661 aaaaaacagg ggggcatcgt tgaccacggt tgcgggtgtg aagccttttt ctagtgcagc
     6721 tgaataaatg aagggtttga agctggagcc gggttggcgc caagcctggg tgacgtggtt
     6781 gaacttgttc ttggcaaagt caaagccccc caccagcgcc ttaatggacc cgtcgcgcgg
     6841 gtccatcgac acaaaagccc cttccacttc tgggagctgg gtgatttccc atgcgccttt
     6901 ggcggttttc gccacgcgaa tgatggcgcc acgacgaacc ttgatgttgg gtggcgcctt
     6961 gtctgaaagt ccggattgtg caggtttgag gccctcgccg gtgatttcca gcgtttcacc
     7021 actttggcgg atcgccttga tgtgttttgc gtctgcttcc agaacaaccg cggacatgac
     7081 gtcgccgttg tccgggtgct catccagggc atcgtcgatg gcgtcctcga tctcctgggc
     7141 ggtcgatgga agtgtgatga atttctcggg gccacggtag atctgacggc gttcaaaatc
     7201 catgatcccg cgtcgcaagg ctcggtaggc ggcgtcctgg tcggctgttc tgagtgtggt
     7261 gtacacattg aggccgcggg tgtaggtttc atcgccatac tgtgcgtaca ttagctggcg
     7321 caccatttcg gttgcatatt cggcatgaac ctgcttcgtg ttcgctccgg tcctgatctt
     7381 cagttcctgt ttttttgctg tttccgcttc cactgcggta ataaatccgt tttcgagcat
     7441 gcgttcaatg atgtagagct gtcttgaacg ggcccttttc ggattggtga tggggttgta
     7501 ggctgacggc gccttgggca ggccggccag catggctgct tcggcaatcg tgatgtcttt
     7561 gagtggcttg ccaaagtagg cttcggaggc tgcggcgaat ccgtaggccc tgtggcccag
     7621 gaaaatctgg ttcatataga tctcaaggat ctggtctttt gtaagcaaat gttccagttt
     7681 gaacgtaagc agaatctcgt aaatcttccg ggtgaaggtt ttttcggatg tcaggtacac
     7741 gttgcgggcg acctgcatgg tgatcgtgga cgcgccctga ctcttgacac gtccgacgtt
     7801 ggcgagtccg gcgcgaatga caccgatgta gtccacgcca ccgtgctgat agaagcgggc
     7861 atcttcaatg gccaaaactg cattggccat gaccgggggg atttccttga tgggtgtcag
     7921 gttgcggcgt tcttcgccaa attcacctag cagatcaccc tcggcagaga acacccgcag
     7981 gggcagcttt gggcggtagt ccgagagatc ggatatgtcc ggcaaattag gaaaagcgac
     8041 agaaagcgcc aacgcaatga cgagcagtag ggacagtgcc ccggccatcg ccagtccccc
     8101 aatccacagt accacccgaa ccagccaatg gtgctgcgag ccgttgccag cttttggcga
     8161 gtcagatttg ttggagggtg tggccatagg gaagaggttg agtgtgcggc gcgattatag
     8221 aaacgggcat ttcagcggta gagtctcact ttgttcggat ggctcctgcc cattatccat
     8281 aacggctgtc tgtggcatat tcatctaaca gacggtttca gaacgtctgc ttttggcaac
     8341 gcttgcgttg ttccgaattg gaaaaaacct ttgcgggaca aggagcttac tgatagcatt
     8401 caagtgaatt cttaa
//
LOCUS       DAOSJM010000055         8281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8056_length_8281_cov_3.637248, whole genome shotgun sequence.
ACCESSION   DAOSJM010000055 DAOSJM010000000
VERSION     DAOSJM010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8056_length_8281_cov_3.637248"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /gene="ftsY"
                     /locus_tag="PKX82_03135"
     CDS             <1..850
                     /gene="ftsY"
                     /locus_tag="PKX82_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOF51272.1"
                     /translation="SSISGVFGGSQIDEELYENLEGALLMADAGVAATETLIADLRQK
                     AKTSGITHPTALKNALITSLSELLQPLQKPLIIGQHQPTVVMVAGVNGAGKTTSIGKL
                     TKHLADHGASVLLAAADTFRAAAREQLTVWADRNTVEIISQEGGDPAAVSFDAVTAGK
                     ARKKDVVLIDTAGRLPTQLHLMEELKKIKRVIQKAEGTAPHEVLLVIDGNTGQNAVAQ
                     VKAFDDALGLTGLIVTKLDGTAKGGVLAAIARERPIPVYFIGVGEKLDELETFNAREF
                     AQALLA"
     gene            867..2078
                     /locus_tag="PKX82_03140"
     CDS             867..2078
                     /locus_tag="PKX82_03140"
                     /inference="COORDINATES: protein motif:HMM:NF008633.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF51273.1"
                     /translation="MSMTLNRRQWLTRIGAPALLSMSFPPAALAEPAWTAIENRAKGQ
                     TVYFNAWGGAETINAYIQWAAAEVQKRYGVTVQHVKITDAADMVKRVRNEKAAGKANG
                     TVDLVWINGENFLTMKREGLLFGPFAESLPSYAKVDTQGKPTTRLDFAEPTEGMEAPW
                     GMAQLTFMADSKRTPKPPRNLTELLTFAKANPGRVTYPRPPNFHGTTFVKQLLVDLNA
                     NRSALYKPVTPEAFAKATAPLWSTLDQLHPHLWRAGKQFPQNAESMRQMLADGELQLA
                     LTFNPNDAANEIAAKRLPASVISYQFSSGTIGNTHFVAIPVNASAKEGAQVFANFLLS
                     AEAQARKADIAVWGDPTVLALDKLSSQERAAFAARPLPGQVEKSAPAIPEPHGSWVDA
                     LEKEWIKRYGV"
     gene            2159..3259
                     /locus_tag="PKX82_03145"
     CDS             2159..3259
                     /locus_tag="PKX82_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOF51274.1"
                     /translation="MKKLFQLLGILVVAAVAYLAIWPVPIQPVAWDAPAAPGYTGPHA
                     ANTKLANLQMIDLKGEIGPEHIVIGPNGKLYTTVASGNILRMNPDGTAQEVFANTGGR
                     VLGFDFDAQGNLIAADAVKGLLSIAPDKQVTVLTDSVNGDPIRYADAVVVAKNGKMYL
                     SDASARFAPKDWGGTFEASVLDILEQASTGRILEFDPATRTTRVVAKGISFANGVALS
                     QDEQTLFVNETGKYRVWKIAVAANNLDVAQPGAQASVLLDNLPGYPDNLMRGLDGKIW
                     LGLAKPRNPTIDKLAQKPWMRSLTLRLPRALWPIPKAYGHVMAFTEDGKVVVDLQDPS
                     GTYPETTAITETKDRLYVQSLHAHGMGWLKKD"
     gene            complement(3263..3628)
                     /locus_tag="PKX82_03150"
     CDS             complement(3263..3628)
                     /locus_tag="PKX82_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOF51275.1"
                     /translation="MCQIFVRANAQSYEATTRSIRLHGVVTSVRLENLFWDTLEEIAQ
                     RDAMHVPQLLSKLYDELLEHRGDIPNFSSFLRVCCLRYLALQVDERIPRDIGIPIGSL
                     NAHAVLRGLRPNLVDANAA"
     gene            complement(3794..>4345)
                     /locus_tag="PKX82_03155"
     CDS             complement(3794..>4345)
                     /locus_tag="PKX82_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOF51276.1"
                     /translation="DYGEDYETMVPFQTLLSIGYTVHAVCPDKKAGDYVMTAIHDFEG
                     AQTYSEKPGHRFTLNANFADVNTAQYDGLLIPGGRGPEYLRLNARVLAITKEFADAGK
                     PIAAVCHGAQLLAAVPGIIKGKTISAYPACRPEVELAGAKFADIAIDAAVTDGQYVTA
                     PAWPAHPAWLAQFIQLLGTKISY"
     assembly_gap    4346..4445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4446..5740)
                     /locus_tag="PKX82_03160"
     CDS             complement(<4446..5740)
                     /locus_tag="PKX82_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF51277.1"
                     /translation="MTGTPTPLQIVHRLWYGPDLVHVQARRERRALMLGSLSMTVMGM
                     VWAGLFITLGNWPMVFLDAVMVLGGLGTGILVWRKKAHAAAVVLLSTLFIVICVIASV
                     FDISTPQAPRTTHLYLLPLGVAALMAFRTASPWLRHGIAVACLAGVAVLSVTYGSILP
                     EYALPPHIRAPGAWVTSIAALTILYGMLHVMQNDAVMRSILENDLQLALAQDQFELHY
                     QPQHDLQGNVIGAEALIRWRHPSRGLVMPGQFIEVAEQTGQILQIGQWVTRQACEQLA
                     RWAQQPECRHLRVAVNISQLQFRQPDFVARILDTIDTFGIDAHKLELELTESMLAEDL
                     QDIIEKMSTIRSRGVTFSLDDFGTGYSSLNYLKRLPLHQLKIDQSFVRDVLADPNDAS
                     IVRTVIALGHNLGLTVIAEGVETEAQRLFLAESGCELFQG"
     gene            5866..6546
                     /locus_tag="PKX82_03165"
     CDS             5866..6546
                     /locus_tag="PKX82_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOF51278.1"
                     /translation="MTTLLPRIELESAPNPTAAVIWLHGLGADGNDFAPIVPELDLGG
                     CPAIRFVFPHAPSIPVTINGGYVMPAWYDILGVNLVDRQDDKGIQKSEWAIQALIQHE
                     VDRGIPHARIVLAGFSQGCAMALHTGLRLPHRLAGIMALSGYLPLADRFPSERHAANA
                     TTPIFMAHGTQDPVVVLQRGEDSRDALAKLGHPVEWHTYPMQHSVHPREIADIGRFLT
                     KVLAPSVA"
     gene            6543..7796
                     /locus_tag="PKX82_03170"
     CDS             6543..7796
                     /locus_tag="PKX82_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOF51279.1"
                     /translation="MNPLVLGIDFGTSNSAAALIDAQGQLQVIPLDGARAEMPTALFF
                     ASETHTVLYGSEAMQAYLNGTEGRLLRSLKSLLGSRLMDEYTAVGDKSIRFFDIVVLF
                     FKELKRRCEAHVGQPLTHAVLGRPVHFVDDSPERDALAQETLGRAAMEAGFTHIAYQL
                     EPIAAALDYEQRVDKETIALVVDIGGGTSDFTVIRLNPERSALSDRSADILATTGVHI
                     GGTDFDRLLDLSTVMPYLGYKHVGTGGRIVPSSVFFDLSSWHLIHQSYTRKAMHFAKE
                     LWTDYTDQALHRRLMDALEEQHGHRMLASVEAAKIACSISGDSATVNLDFLDRTLTPA
                     INAQSMERALHDSIHQVVQCAQECVAAAGLQGVDAVYLTGGSSALRTLIEALRQAMPN
                     ATLVEGNRFGGVAAGLAWAGAVQRY"
     gene            complement(8080..>8281)
                     /locus_tag="PKX82_03175"
     CDS             complement(8080..>8281)
                     /locus_tag="PKX82_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51280.1"
                     /translation="KFAAKFSSKWLSFQPESTGNIDWRALDDSFFEFPPSCPELIPCN
                     PNDGMAELQVQVLKQWLAHRMI"
BASE COUNT         1637 a         2648 c         2477 g         1419 t
ORIGIN      
        1 gtccagtatt tccggcgtgt ttggcggcag ccagatcgac gaggagctgt acgagaatct
       61 ggaaggtgct ctgctgatgg ccgacgcggg cgtggccgcc accgagacac tgatcgcgga
      121 cctgcgccag aaggccaaga ccagcggcat cacgcacccc accgcactga agaatgcgtt
      181 gattacgtcg ctgtccgagt tgctgcagcc gctgcaaaag ccgctgatca ttggccagca
      241 ccagcccacc gtcgtcatgg tggcgggcgt caacggtgcc ggcaagacca ccagcattgg
      301 caagctcacc aaacacctgg ccgaccatgg cgccagcgtg ctgctggccg ctgccgatac
      361 cttccgtgct gcggcccgcg agcagctcac cgtctgggct gaccgcaaca cggtagagat
      421 catcagccag gaaggcggcg accccgcagc cgtgagcttt gatgccgtga ccgccggcaa
      481 ggcgcgcaag aaagacgtgg tgctgatcga caccgccggc cgcctgccga cccagctgca
      541 cctgatggaa gagctcaaga agatcaagcg cgtgattcaa aaagcggagg gcaccgcgcc
      601 gcatgaagtg ctgctggtga ttgacggcaa caccggtcag aacgccgtgg cccaggtcaa
      661 ggcctttgac gatgcgctgg gcctgaccgg cctgatcgtc accaagctcg acggcaccgc
      721 caagggtggt gtgctggcag cgattgcacg cgagcggccc attccggtgt atttcattgg
      781 cgtgggcgaa aagctcgacg aactcgaaac cttcaacgca cgggaatttg cgcaggcgtt
      841 gttggcctaa ccccaggaaa caccccatgt ccatgacact caaccgccgc cagtggctca
      901 cccggatcgg tgcgcccgcc ctgctttcca tgtcatttcc gcctgcagcc ctcgcggaac
      961 ctgcgtggac agctatagaa aacagagcaa aaggtcagac ggtgtacttc aacgcctggg
     1021 gcggtgccga gaccatcaac gcctacatcc agtgggccgc ggccgaggtg caaaagcgct
     1081 atggtgtgac ggtgcagcat gtgaagatca ccgacgcggc ggacatggtc aaacgtgtgc
     1141 gcaacgagaa ggctgccggt aaagccaacg gcacggtcga cctggtatgg atcaacggcg
     1201 agaacttcct gaccatgaag cgcgaaggcc tgctgtttgg cccgtttgcc gaaagcctgc
     1261 cgtcgtatgc caaggtggac acgcagggca agcccaccac acgactggac tttgccgagc
     1321 ccaccgaggg catggaagca ccctggggca tggcccagct caccttcatg gccgacagca
     1381 agcgcacgcc caagccaccc cggaacctga ccgagctgtt gacctttgcc aaggccaacc
     1441 caggccgcgt gacctacccg cgtccgccca acttccatgg cactacgttt gtgaagcagc
     1501 tgctggtgga cctgaacgcc aaccgcagcg cgctctacaa gccagtcacg cccgaagcat
     1561 ttgccaaagc gactgccccc ctgtggtcca cgctggacca gctgcacccg cacctgtggc
     1621 gcgctggcaa gcagttcccg cagaacgctg aatccatgcg ccagatgctg gccgatggcg
     1681 agctgcagct ggcactgacc ttcaacccca acgacgcggc caatgaaatc gccgccaagc
     1741 ggctccctgc cagcgtcatc agctaccaat tcagtagcgg aaccattggc aacacgcact
     1801 tcgtagcgat cccggtgaac gcctccgcca aggagggtgc acaggttttt gccaacttcc
     1861 tgctcagcgc cgaggcccag gcgcgcaagg ccgacattgc cgtgtggggc gacccgacgg
     1921 tactggcgct ggacaagctc agcagccagg agcgcgccgc ctttgccgcc aggcccctgc
     1981 ccggccaggt ggagaagtct gccccggcca ttcccgagcc gcatggcagc tgggtggatg
     2041 cgctggaaaa ggaatggatc aagcgctacg gcgtgtgact gcctccgagg tgacagccaa
     2101 ccgccggctt tcgcccacaa tcggtcgctt cccataacaa cgaaagagga gacggttcat
     2161 gaaaaagcta ttccagttat tgggcattct ggttgttgcg gcggtggcct acctggcaat
     2221 ctggccggtg cccatccagc cagtagcctg ggacgcgccg gcagcgccgg gctacaccgg
     2281 gccgcacgca gccaacacca aactcgccaa cctgcagatg atcgatctca agggtgagat
     2341 cggccccgag cacatcgtca ttggccccaa cggcaagctc tacaccacgg tggccagcgg
     2401 caacatcctg cgcatgaacc ccgatggcac ggcccaggaa gtgtttgcca acacgggcgg
     2461 gcgcgtgctc ggttttgact ttgacgccca aggcaacctg attgccgccg acgcggtcaa
     2521 gggcctgctg tccatcgcgc cagacaaaca ggtcacggta ctgaccgaca gcgtcaatgg
     2581 cgacccgata cgctacgccg atgccgtggt ggtcgcaaag aacggcaaga tgtacctgag
     2641 cgatgcctcg gcccgctttg cgcccaagga ctggggcggc acctttgagg ccagcgtgct
     2701 ggacatcctg gagcaggcca gcacgggccg catcctcgaa tttgaccccg ccaccaggac
     2761 cacccgtgtc gtagccaaag gcatcagttt tgccaacggc gtggcgctga gtcaggacga
     2821 gcagaccctg tttgtgaacg aaaccggcaa gtaccgtgtg tggaaaattg cggtagccgc
     2881 caacaacctg gacgtggcgc aacctggcgc gcaggccagc gtgctgctgg ataacctgcc
     2941 cggctacccc gacaacctga tgcgtgggct ggacggaaag atctggctgg gtctggccaa
     3001 accgcgcaac cccaccatcg acaaactggc acagaagccc tggatgcgca gcctgaccct
     3061 gcggctgccg cgcgccctgt ggccgattcc caaggcctac gggcacgtga tggcgttcac
     3121 cgaagacggc aaggtggtgg tcgacctgca ggaccccagt ggcacctatc ccgagaccac
     3181 cgcgatcacc gaaaccaagg accgcctgta tgtgcagagc ctgcacgcgc acggcatggg
     3241 ctggctcaaa aaggactaga tctcaggctg cgttcgcgtc caccaggttg ggccgcaggc
     3301 cccgcagcac ggcgtgggcg ttgagcgagc caatcggtat gccgatgtcg cgcggaatgc
     3361 gctcatccac ctgcagggcc aggtagcgca ggcaacacac gcgcagaaag ctggagaaat
     3421 tggggatgtc gccccggtgc tccagcaact cgtcgtacag cttggacagc aattggggca
     3481 catgcatggc atcgcgctgg gcaatttctt ccagcgtatc ccagaacagg ttctccagcc
     3541 gcacgctggt caccacgcca tgcagccgga tgctgcgcgt ggtggcttca tagctttgcg
     3601 cattggcgcg cacaaaaatc tggcacatgg acggctcccc ttcaatggct gcgacacccg
     3661 gccccagagc atacgccctg tgacgctgca acgctgcgtc cggccgcaga aactcgccgc
     3721 cgggccgccc caaggcgagt tagcctcctt ggggggcagc gacccgcgca gcggcggagc
     3781 gtgggggctc tgtttagtac gagatttttg tacccagcaa ctggatgaac tgcgccagcc
     3841 acgccggatg ggcgggccag gccggtgcgg taacgtattg gccatcggtc actgcggcat
     3901 caatggcaat gtcggcaaat ttggcacccg ccagctccac ttcggggcgg caggctgggt
     3961 aggcgctgat ggtcttgccc ttgatgatgc cgggcaccgc ggccagcaat tgcgcgccgt
     4021 ggcagaccgc ggcgatgggt ttgccggcat cggcaaactc cttggtgatg gcgagcacgc
     4081 gggcattgag ccgcaggtac tcggggccgc gtccgccggg gatcagcaag ccgtcgtact
     4141 gcgcggtgtt gacgtcggca aaattggcgt tgagcgtgaa gcggtggccc ggcttctcgc
     4201 tgtaggtctg ggcaccttca aagtcgtgga tggccgtcat tacgtagtcg cctgccttct
     4261 tgtccgggca caccgcatgc acggtgtagc cgatggacag cagggtctgg aatggcacca
     4321 tggtttcgta gtcttcgcca tagtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnccctg gaacaactcg cacccggact ccgccagaaa cagccgctgc gcctcggttt
     4501 ccacgccttc tgcaatcacc gtcagcccca ggttatgtcc cagtgcgatc acggtacgca
     4561 caatcgacgc atcgttcggg tcggccagta cgtcgcgcac aaaggactgg tcaatcttga
     4621 gctggtgcag gggcagtcgc ttgaggtagt tcaaggacga ataccccgta ccgaaatcgt
     4681 ccagtgagaa cgtgaccccg cggctccgga tggtcgacat cttttcaatg atgtcctgca
     4741 ggtcctcggc cagcatggac tcggtcaact caagttcgag cttgtgtgca tcgatgccaa
     4801 aggtatcgat ggtgtccaga atccgggcca cgaagtctgg ctggcggaac tgcagctgac
     4861 tgatgttgac ggccacccgc agatggcggc attccggctg ctgcgcccag cgcgccaact
     4921 gctcgcaggc ctgcctggtc acccactgcc cgatctggag aatctgcccc gtctgctcgg
     4981 ccacttcgat gaactgaccc ggcatgacca gaccgcgact gggatgcctc catcggatca
     5041 acgcttcggc cccgatgaca ttgccttgaa gatcgtgttg tggctggtag tgcaattcga
     5101 actggtcctg cgcaagcgcc agctgcagat cgttctccag aatggagcgc atcacggcat
     5161 cgttttgcat gacgtgcagc atgccgtaca gaatggtcag cgcagcaatg cttgtcaccc
     5221 acgccccagg cgcccggatg tggggcggca gcgcatattc aggcagtatg gaaccgtagg
     5281 tcaccgacaa cacggccacg cccgcaagac aggctaccgc aatgccgtgg cgcagccagg
     5341 gactcgcggt gcgaaacgcc atcagggcgg ccacccccaa tggcagcaga tacagatggg
     5401 tggttcgggg ggcttgcggc gtggagatgt caaacacgga cgcgatcacg cagatcacga
     5461 tgaacagcgt ggataacagc accaccgcag ccgcatgcgc cttcttgcgc cagaccagga
     5521 taccggtgcc cagcccaccc aaaaccatca ccgcgtccag aaacaccatc ggccagttgc
     5581 caagggtgat gaagaggccg gcccagacca tgcccatcac cgtcatggag agactgccca
     5641 gcatcagcgc gcgccgctcg cggcgcgcct gaacatgcac cagatccggg ccataccaca
     5701 accggtggac tatctggagc ggggtggggg tgccagtcat gttcggatgc gtttcaaaaa
     5761 cattgtaggc gtggctttgc acagctgcca agcccctcgc gtcagcgtac ggagcttcgt
     5821 ggacaataga gccccccggt tgaccccacc gcccaaggcc acgccatgac caccctgctt
     5881 ccccgcatcg aactcgaatc cgcccccaac cctaccgccg ccgtcatctg gttgcacggc
     5941 ctgggcgctg acggcaacga ctttgcgccc atcgtccccg agctggacct gggcggctgc
     6001 ccagccatcc gctttgtctt tccgcacgcc ccgagcattc cggtcaccat caatggcggc
     6061 tatgtgatgc ccgcctggta cgacatcctg ggcgtcaacc tggtggaccg ccaggacgac
     6121 aaaggcatcc agaaatccga atgggcgatc caggcactga tccagcatga ggtggaccgt
     6181 ggcatccccc acgcgcgcat cgtgctggcc ggcttcagcc agggctgcgc catggccctg
     6241 cacaccggcc tgcgcctgcc gcaccggttg gcgggcatca tggccctgtc gggctacctg
     6301 ccgctggccg accgctttcc cagcgagcgc catgcggcca acgccaccac acccatcttc
     6361 atggcgcacg gcactcagga cccggtggtc gtcctgcagc gcggcgaaga ttcgcgcgac
     6421 gccctggcca aactgggcca cccggtggag tggcacacct accccatgca acacagcgtg
     6481 cacccgcgcg agattgccga cattggccgc ttcctgacca aggtgttggc cccctcggtc
     6541 gcatgaaccc actcgttctg ggcattgact ttggtacctc caactctgcc gccgcactga
     6601 ttgacgcgca ggggcaattg caggtcatcc cgctggatgg cgcgcgggcc gaaatgccca
     6661 ccgccctgtt ttttgccagc gaaacccaca ccgtgctcta tggcagcgag gccatgcagg
     6721 cctacctgaa cggcaccgag ggccgcctgc tgcgctcgct caaaagcctg ctgggcagcc
     6781 gcctgatgga cgagtacacc gcggtgggcg acaagagcat ccgcttcttc gacatcgtgg
     6841 tgctgttctt caaggagctc aagcgccgct gcgaagccca tgtgggccag ccgctgacgc
     6901 acgccgtact gggccgcccg gtgcactttg tggacgacag tccggagcgc gacgcactgg
     6961 cgcaagagac cctgggccgt gccgccatgg aagcgggctt cacccacatt gcctaccagt
     7021 tggaacccat tgctgccgcg ctggactacg agcagcgcgt ggacaaggaa accattgcgc
     7081 tggtagtgga cattggtggt ggcacgtccg acttcactgt catccgcctg aaccccgagc
     7141 gcagcgccct gagcgaccgt agcgcagaca tcctggcgac caccggcgtg catattggcg
     7201 gcaccgactt tgaccgcctg ctggacctgt ccaccgtgat gccctatctg ggttacaagc
     7261 acgtgggcac cggcgggcgc attgtgccca gcagcgtatt ttttgacctg agcagctggc
     7321 acctgatcca ccagtcctac acgcgcaagg cgatgcactt tgccaaggag ctgtggaccg
     7381 actacaccga ccaggccctg caccgccgcc tgatggacgc gctggaagaa cagcacggcc
     7441 accgcatgct ggccagtgtg gaggccgcca agattgcctg ctccatcagc ggtgacagcg
     7501 ccacagtgaa tctggatttt ctggaccgca cactcacacc cgccatcaat gcacaaagca
     7561 tggagcgggc cctgcatgac tccatccatc aggtggtgca gtgcgcacaa gagtgcgtgg
     7621 ccgctgcggg cctgcaaggc gtggatgcgg tgtacctcac cggcggttca tcggccctgc
     7681 gcacgctgat cgaggcgctg cgccaggcca tgcccaacgc cacgctggtg gaaggcaacc
     7741 ggtttggcgg cgtggccgcg gggttggcct gggcgggcgc cgtgcagcgg tactagattg
     7801 ctactatttt gatagctctc tgtgcatatt ggacgggggc tacatgcact tttcatacaa
     7861 atatttggcg aagcggagca aaacttcacc aacatcgcgc cagatgtctg acactttcgc
     7921 agcggttgca acctttcaga tcacctgttt ctttgactgt gatgggccat gtgcagtcgt
     7981 tggtgaatga ccggtaactg gaagccaatt ggtcatcgca gtcgctcaat tttcaatggc
     8041 ctgtgagttc gatatgtttg cggtgtcaat cgatgcagtt caaatcattc tgtgagccaa
     8101 ccattgcttc aggacttgca cttgcaattc cgccattcca tcatttgggt tgcacggaat
     8161 cagttctggg caactagggg ggaattcaaa gaaagaatca tccaaagcgc gccaatcgat
     8221 gttgcctgtt gattccggtt gaaaactgag ccactttgag gaaaatttcg ctgcaaattt
     8281 g
//
LOCUS       DAOSJM010000056         8265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8077_length_8265_cov_4.112302, whole genome shotgun sequence.
ACCESSION   DAOSJM010000056 DAOSJM010000000
VERSION     DAOSJM010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8265
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8077_length_8265_cov_4.112302"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKX82_03180"
     CDS             <1..309
                     /locus_tag="PKX82_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HOF51281.1"
                     /translation="VVVTGLILDKLIPGLGILRGDMQVAHMIHSVLALWMIALLLGHI
                     YMGTVGVRGAYKAMKTGYVSEGWAHEHHELWYDDIKTGKIPAERSAQGAPGSGAGVQA
                     "
     gene            327..689
                     /locus_tag="PKX82_03185"
     CDS             327..689
                     /locus_tag="PKX82_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51282.1"
                     /translation="MKSVIVFLAGTALTMAAVAKLPAPSDEAKAKAAEAAAKTAHAGK
                     VDAYKLCKSQDKVAAHYQKGSKTASKDKKDAVATPACADPGAFVYVPAAAASAPAAAA
                     APAAQTAAAPAATDKKKP"
     gene            759..1610
                     /locus_tag="PKX82_03190"
     CDS             759..1610
                     /locus_tag="PKX82_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOF51283.1"
                     /translation="MSEFSVPYLTQARLPLTHALDAVNEFGETIAMAIPSERALTLYV
                     DKREIVTLMTLGAQPEWLVLGFLLNQRLVRSAQDVESITVDWEVGAAAVKTRKGIANL
                     KEKTSRRTVTSGCGQGSMFGTLMDAVEQIELPAATITQGQLYAIVNAIRLQESTYKAA
                     GSVHGCALFQGEEMLLFVEDVGRHNAIDAIAGWMALQPGMRSQDKFFYTTGRLTSEMV
                     IKAAQMGIPVVVSRSGITQMGHMVAQRLGLCAIGRATNKRFLCFTGAHRLVLQPELAG
                     THLRVVA"
     gene            1682..4201
                     /locus_tag="PKX82_03195"
     CDS             1682..4201
                     /locus_tag="PKX82_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51284.1"
                     /translation="MNLSFWRLGWRTLWRDLRSGDLRLVMVAVVLAVAALTAVGFFAD
                     RLKGGLQRDARQLLGGDAVVSSDNPTPQIVLDKARSLGLVVNSTLGFPTMARAAEAQG
                     GATKLVALKAVEEGYPLRGSLLVSADPDAAERPTRDVPRPGQAWVDAPLLESLGLKLG
                     DSLLLGESTLKITSVIVVEPDRGAGFMNFAPRVMVHQADVAATKLIQPASRINYRMAV
                     AGANPAVKVFVDWADAQVKAGTVRGVRVESLQSGRPEMSQTLDRAEKFLSLVALLAAL
                     LSAVAVALAARAFAASHLDDCAMLRVLGLSQRTIASSYAFEFALVGVFASAVGVLLGY
                     IVHYLFVVLLAGLVSTDLPAATLWPVALGMGMGLTLLFAFGLPPVLQLAQVPPLRVIR
                     RDVGNLRPASLGVLSVGVLGFAALLLAASSDITLGLIAVGGFAGAVLVFASLSWMAVK
                     VLRRSVNETTAPRWLVLATRQISARPAYTVVQVSALAVGLLALVLLVLLRTDLIGSWR
                     KATPPDAPNRFVINVMPEQGDAFRQALDKAGVAKYDWYPMIRGRLVAVNGRAVSPEDY
                     AEDRAKRLVDREFNLSHSARKPDNNTLVGGAWTEEEQGAISVEEGIAKTLNLKLGDSL
                     RFDIGGMQTESKITSLRKVDWGSMRANFFVMYPVSRLENVPSTYMGAFKAPATPGFDN
                     ALVRQFPNVTNVDMTSTINQVQRVLDQVIRAVEFLFGFTLAAGLVVLFAAVTATREDR
                     AKEFAVMRAVGAQGSLLRQVQRTELAGVGFLAGTLASVVASGVGWALARFVFEFEWTV
                     SLWVPLFGAIAGAVLALAAGWWGLRDVLRRPVVDTLRRATT"
     gene            4418..4759
                     /locus_tag="PKX82_03200"
     CDS             4418..4759
                     /locus_tag="PKX82_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51285.1"
                     /translation="MKYKEEPLLTPQHLVEAEGLGIKLARLRRARKIRQADAAARAGL
                     ARSTAVLIEKGDPSRTLGQIFRYLEAIAPGLSLPALLQESDPSLTTLAQAEATQRVRP
                     MSAAELQALDF"
     gene            4759..6096
                     /locus_tag="PKX82_03205"
     CDS             4759..6096
                     /locus_tag="PKX82_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOF51286.1"
                     /translation="MAQRSLYVFTHLTEGWAPAGRLSLTQERDVVASSFAYGIRYIDR
                     PDAFEIDPVSLSLADKARVRGAELFPANQLAQFGGIRDAAPDAWGRRVIEAQRRVPAN
                     SLSEADYLLGAGSDRVGALDVRESLDSPVQAGAAPIRSLPYMLEAAERIEQGLPLPAA
                     LADVFGAGPSAGGARPKASVRDEAGVLWLAKFAAVGDTLDMAWAECCTLQLAGRCGLT
                     VPEVKVMDIGGQSVLLVRRFDRTAAVSGVEQRLPFVSGLTLVACDEFDSRLKGYGHLA
                     QAIREHVHPSHIRANCEELFARMVFNIFVSNDDDHLRNHGFLRHTVERGQLPGWSLSP
                     LYDVVPRPSMAFERQLHLQVGQQGKLATLDNAMSAYSAFTPQRTTAVAIIRRIWGELR
                     QWRTTFEALGASNPGLGRLLDQLAPAMRSLDDIASPALQAEIRKNRTPEYQNL"
     gene            6093..6497
                     /locus_tag="PKX82_03210"
     CDS             6093..6497
                     /locus_tag="PKX82_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HOF51287.1"
                     /translation="MSDEDEPQGRSAFDWIGGEAAIRTLVDRFYDLMDLEPAYRELRA
                     AHGSTLDDARDKLFWFLCGWMGGPQHYIERFGHPRLRARHLPFKIGILERDQWLACMD
                     QAMGDVEVAADLRARLRESFFQTADWMRNQHV"
     gene            complement(6494..7621)
                     /locus_tag="PKX82_03215"
     CDS             complement(6494..7621)
                     /locus_tag="PKX82_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF51288.1"
                     /translation="MSTAPAHLPAANASMRLEAIAHARQTLLVERRAPASLQLSPWVE
                     RSWQRCMALGHDPQRDIVFEVLTPQQMRHTTERNAQLVQSARPLLENLARAIADTRYF
                     AILTNADGVVVDACGAIDHSDPRADMVTRIGTDLSERSVGTTAIGAALTDLQPVWLHR
                     GEHFFANTGHYSCAGAPLMGPDGHCIGMLDLTGIDALERPELKHLAAQTASKIENALV
                     LDQPHSMTLRLNWPGNTMGGDTDGLLCLDSEGWITAANPAARMMLSGLHTARGQAVHV
                     SDVFGMPYQMLFDAARRARTVLDIPLWTGLHLQALPVQASEDKRPIPMHALDQRGVPL
                     KTREVEVIRRSVEEARGNVAQAAQALGISRATIYRKLGRKH"
     gene            7836..>8265
                     /locus_tag="PKX82_03220"
     CDS             7836..>8265
                     /locus_tag="PKX82_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOF51289.1"
                     /translation="MQKMLHINPDKCTGCLQCEMACSFENYGTFATAKSRIKVFEFHQ
                     TGKKVPYTCTQCDEAWCLHACPVEAITVDKATGAKVVNEATCVGCKVCTIACPFGTIN
                     YVQETGKVQKCDLCGGDPACATACTTGAITFIDANWTGLGK"
BASE COUNT         1436 a         2462 c         2708 g         1659 t
ORIGIN      
        1 gtggtcgtta cgggattgat tctcgacaag ctgattcctg gtctgggcat tctgcgtggc
       61 gatatgcagg tggcccacat gatccattcg gtgcttgcgt tgtggatgat cgctttgctg
      121 ctggggcaca tttacatggg tactgtgggt gtgcgggggg cctacaaggc catgaaaacc
      181 ggctatgtat cggaaggctg ggcgcacgag catcacgaac tctggtacga cgacatcaaa
      241 accggcaaaa ttccggcaga gcgctctgcg cagggcgcgc caggctccgg cgctggcgta
      301 caggcatgaa ttccaaagag gcacacatga aatccgtaat cgtctttctg gcaggtacag
      361 cattgaccat ggccgcggtg gcaaaactac cagcacccag tgacgaagcc aaagccaagg
      421 ccgcagaagc tgctgccaag accgcccatg ccggtaaagt agatgcctac aagctgtgca
      481 agtcgcagga caaggtagct gcgcattacc aaaagggttc caagacggcg agcaaagaca
      541 agaaagatgc ggtcgcaaca cctgcatgtg ctgaccccgg agcctttgtc tacgttcctg
      601 cggccgctgc gtctgctcct gcagccgctg ctgcgccggc cgcacagaca gctgcagcac
      661 ctgcagccac agacaagaaa aagccctgag agtcggcttt gcgcgttcca cgcagcccca
      721 ggcgcgacgt gccggggctt ttttgttagt ctctcaccat gagtgaattt tctgtgccct
      781 atctgacgca ggcccggctg cctttgacgc acgctttgga cgcagtgaat gagtttggtg
      841 agaccattgc catggccatt ccctcggagc gtgcactcac cctctatgtc gacaagcgcg
      901 agattgtgac cctgatgaca ctgggcgcac agccggaatg gctggtgctc gggttcctcc
      961 tgaaccagcg tctggtgcgg tccgcgcagg atgtggagtc cattacggtg gattgggagg
     1021 tcggtgccgc tgcggtcaag acccgtaagg gcattgcgaa cctgaaagag aaaacgtcca
     1081 ggcgcaccgt gacatcgggg tgcggacagg gcagcatgtt tggcaccctg atggacgcgg
     1141 tcgagcagat tgaactgccc gctgccacca ttacccaagg ccagctgtat gccatcgtca
     1201 acgccatccg tttgcaggaa agtacctaca aagccgcagg ttccgtgcat ggttgtgctc
     1261 tgttccaggg ggaagagatg ctgctgttcg tggaggacgt agggcggcac aacgccatcg
     1321 atgccatcgc aggctggatg gcactgcagc ccggcatgcg aagccaggac aagttttttt
     1381 acaccaccgg ccggctcacc agtgaaatgg tcatcaaggc ggcccagatg ggtattcccg
     1441 ttgtggtgtc acgtagtggc atcacccaga tgggccacat ggtggcgcag cgcctgggcc
     1501 tgtgcgccat cgggcgggcg accaacaagc gttttctgtg ctttaccggt gctcaccggc
     1561 tggtattgca gcccgagctc gccgggacgc acttgcgggt cgtggcctga atccccgctg
     1621 ccggctgggt tctatcgggc cctgcaggta gcacgcgtcc cagatgcgct aaggtaaggg
     1681 gatgaatctt tctttttggc gtctgggttg gcgaacactg tggcgtgatc tgcgctccgg
     1741 ggatttgcgt ctggtcatgg tggcggtggt gctggcagtg gctgcattga ccgccgtagg
     1801 attttttgca gaccggctca agggcgggct gcaacgcgat gcgcgacagc tcctgggcgg
     1861 agacgccgtg gtgtcgagcg acaaccccac cccgcagatc gttctggaca aggcacgttc
     1921 tttgggtctg gtggtgaatt ccaccctggg ttttccgacg atggccaggg ctgcggaagc
     1981 acagggcggg gccaccaaac tcgtggcgct caaggccgtt gaagagggct atccgctgcg
     2041 tggctccctg ctggtcagtg ctgatccaga cgctgcagag cgacccaccc gcgatgtgcc
     2101 ccgccccggt caggcctggg ttgatgcgcc actcctggag tctttgggcc tgaagctggg
     2161 ggactccctt ctgctgggcg agagtacgct gaagatcact tcggtcattg tggtggagcc
     2221 agaccggggc gcgggtttca tgaattttgc tccacgcgtg atggtccacc aggccgacgt
     2281 agcggctacc aagctgattc agcccgccag tcgcatcaac taccgcatgg ctgtcgcagg
     2341 tgccaaccct gcggtgaagg tgtttgtgga ttgggccgat gcccaagtca aggcagggac
     2401 agtgcgtggg gtgcgcgtgg agtcgctcca gagtggtaga cccgagatga gccagacgct
     2461 ggaccgcgca gaaaaatttc tcagtctggt ggctctgctg gcggcattgc tcagtgccgt
     2521 ggcggtggcc ctggcggcgc gtgcctttgc ggccagccac cttgacgatt gcgccatgct
     2581 gcgggtgttg ggcttgagcc agcgcaccat cgcgtcgagt tacgctttcg aattcgccct
     2641 ggtgggcgtg tttgccagcg ccgtcggtgt gctgttggga tacatcgtgc actacctgtt
     2701 tgttgtgctg ctggcaggac tggtatcgac tgatctgccc gccgcgacac tctggcccgt
     2761 ggcactgggt atgggcatgg gcttgacgct gctgtttgca ttcggcctgc cgccggtact
     2821 gcagctggcg caggtgccac cgctgcgcgt gatccggcgc gatgtgggca atctgcggcc
     2881 cgcgtcactg ggggtgctgt cggtaggggt gctgggtttt gctgccctgc tgctggctgc
     2941 cagcagcgac atcacgctcg gcctgatcgc agtgggcggt tttgctggtg cagtgcttgt
     3001 gtttgcctcg ctgagctgga tggcggtcaa ggtattgcgt cgcagtgtca acgagaccac
     3061 tgccccgcgc tggctggtgc tggccacgcg gcagatctct gcccgacctg catacaccgt
     3121 ggtgcaagtc agtgctttgg cggtgggtct gctggccctg gtgctgctgg tcctgctgcg
     3181 caccgacctg attggcagct ggcgcaaggc cacgccgcca gatgccccca accgttttgt
     3241 catcaacgtc atgccagagc aaggcgacgc gttccgccag gcattggaca aggcgggtgt
     3301 agccaaatac gactggtatc ccatgattcg cggacggctg gtggcggtca acggacgtgc
     3361 cgtgtcaccc gaggactatg ctgaggaccg tgccaagcgg ctggtggacc gtgagttcaa
     3421 cctatcccac agcgccagaa aaccggacaa caacaccctg gtgggcggcg cctggaccga
     3481 ggaggagcag ggagccatca gtgtggagga gggcattgca aaaaccctca acctcaagct
     3541 gggggatagc ctgcgcttcg acattggtgg catgcagacc gaatcgaaaa tcacctcgct
     3601 ccgcaaagtg gattggggtt ccatgcgggc caattttttc gtgatgtacc cggtcagccg
     3661 tctggagaat gttcccagca cctatatggg ggccttcaag gcaccggcga cgccggggtt
     3721 tgacaacgcg ctggtgcgcc agtttcccaa tgtcaccaac gtcgacatga ccagcaccat
     3781 caaccaggtg cagcgcgtgc tggaccaggt gattcgtgct gtggagtttc tgtttggctt
     3841 cacgctggct gcgggtctgg tggtgctgtt cgctgcggtc accgccacgc gggaagaccg
     3901 cgccaaggag tttgcagtca tgcgggcggt gggggcgcag gggagcctgc tgcgccaggt
     3961 acaacgcact gagctggcgg gcgtgggctt tctggcgggc acgctggctt ccgtggtggc
     4021 ctccggtgtg ggctgggcgc tggcgcgctt tgtgttcgag tttgaatgga cggtgtcgct
     4081 ctgggtcccc ttgtttggcg ccattgcggg tgccgtgctg gcgctggcgg caggatggtg
     4141 gggcttgcgc gacgtgctgc gccgcccggt ggtggatacc ctgcgccggg cgaccacctg
     4201 aattgctata tttttgatag ctgcttgcgc ttatttggcg ggggctatag cccatttttg
     4261 tctaaaattc ggggcaaact gcgccgtgcc gttgaacccg tttatgtagc cgagatggct
     4321 tcaaacgaag cacaattggc tacgttacat gccttgtgtt tttcgggaaa tgtccgtaat
     4381 ataggtcctt aagttgtttg ggactgcaat tacagacatg aaatacaaag aagagcctct
     4441 tctgactcct cagcatctgg ttgaagccga gggtcttggc atcaagctgg cccggcttcg
     4501 gcgcgcccgc aagatacgcc aggccgatgc cgctgcgcgc gccggcctgg cccgctcaac
     4561 ggcggtgctg atagagaagg gtgaccccag ccgcacactg gggcagatat ttcgctacct
     4621 ggaagccata gcccccggac tgtcgctccc ggctttgctg caagagagcg acccctcgct
     4681 cactaccctg gcgcaggccg aggccaccca gcgtgtgcgc cccatgtcag cggctgaact
     4741 gcaagcgctg gacttctgat ggcgcagcgc tcgctctacg tctttaccca cctcaccgag
     4801 ggttgggctc ccgcggggcg cctgtcgctt acgcaggagc gcgatgtggt ggcttccagt
     4861 tttgcctacg gtatccgcta tatcgaccgt cccgatgcct ttgaaattga ccccgtcagc
     4921 ttgagcctgg ccgacaaggc ccgtgtgcgt ggagctgaac tgttccctgc aaatcagctc
     4981 gcgcagtttg gcggcattcg cgacgccgcg cccgatgcct ggggccgtcg ggtcatcgaa
     5041 gcccagcgcc gcgtgcctgc caacagcctc tcggaggcgg actatttgct gggcgccggc
     5101 agtgaccgcg tgggcgcact ggacgtgcgc gagtcgctgg acagtccggt gcaagccggc
     5161 gcggcgccca tccgctcact gccctacatg ctggaagctg ccgagcgcat cgagcagggc
     5221 ttgcccctcc cagccgcctt ggccgatgtg tttggcgcgg gccccagcgc cgggggcgcg
     5281 cggcccaagg cctcggtacg tgacgaggcg ggcgtgctct ggctggccaa gtttgccgct
     5341 gtgggcgaca cgctggacat ggcctgggcc gagtgttgca ccctgcaact ggcgggccgc
     5401 tgcggcctca cggtgcccga agtcaaagtc atggacatcg ggggccagtc ggtactgctg
     5461 gtgcgccgct ttgatcgcac agcggccgta tcgggagtgg agcagcgcct gcctttcgtc
     5521 agcgggctga ctctggtggc gtgcgatgag tttgactccc gcctcaaggg ctacggccac
     5581 ctggcccagg ccatccgcga gcatgtgcac cccagccaca tccgcgccaa ctgcgaagaa
     5641 ctgtttgccc gcatggtgtt caacatcttt gtcagcaacg acgacgatca tctgcgcaac
     5701 cacggcttcc tgcgccacac cgtcgaacga gggcagctac ccggctggtc tctaagcccg
     5761 ctgtacgacg tggtaccacg ccccagcatg gcgttcgagc ggcagctgca tttgcaggtg
     5821 ggacagcagg gcaagctggc cacgctggac aatgccatgt ctgcctacag cgccttcacc
     5881 ccgcagcgca ctactgcagt ggccatcatc cgccgcatct ggggcgagtt gcgccagtgg
     5941 cgcaccacct ttgaagcgct gggtgcctcc aatcctggtc tgggccgcct gctggatcaa
     6001 ctggcccccg ctatgcgcag cctggacgac atcgcctcgc ccgcgctaca agccgaaata
     6061 cgcaaaaaca gaaccccgga ataccagaat ttatgagcga cgaagacgaa ccccagggcc
     6121 gcagtgcctt tgactggatc ggtggcgagg cagccatccg cacgctggtg gaccggttct
     6181 acgacctgat ggatctggag cctgcgtaca gggagttgcg cgccgcgcat ggctccacgc
     6241 tggacgatgc gcgcgacaag ctgttctggt ttttgtgcgg ctggatgggc ggcccccagc
     6301 actacatcga gcgctttggg cacccgcgcc tgcgggcgcg gcacctgccg ttcaagatcg
     6361 gcattctgga gcgggaccag tggctggcct gcatggacca ggcgatgggc gatgtggagg
     6421 tggccgccga cctgcgcgcg cgcctgcggg agtcgttctt ccagactgcc gactggatgc
     6481 gcaaccagca cgtctagtgc ttgcggccga gcttgcggta aatcgtggcg cggctgatgc
     6541 ccagggcctg tgctgcctgc gcgacgttgc cgcgggcttc ttccaccgag cgacgaatca
     6601 cttccacctc gcgggttttg aggggcacgc cgcgttggtc tagagcgtgc atggggatgg
     6661 ggcgcttgtc ttctgaggcc tgcaccggca aggcctgcag atgcagtccg gtccataagg
     6721 gaatgtcgag cactgtgcgt gcgcggcggg ccgcgtcgaa cagcatctgg tagggcatgc
     6781 caaacacatc actgacgtgc acggcctggc cgcgggcggt gtgcaggccg gagagcatca
     6841 tgcgtgcggc cgggttggct gcggtaatcc agccttcgga atccaggcac agcaggccgt
     6901 cggtatcgcc acccatggtg ttgccgggcc agttcagccg cagcgtcatg ctgtggggct
     6961 ggtccagcac cagcgcgttc tcgatcttgc tggccgtctg ggcggccaga tgcttgagtt
     7021 cggggcgctc caaggcgtcg attccggtca ggtccagcat gccgatgcag tggccatcgg
     7081 ggcccatcaa gggggcgccg gcacagctgt aatgccccgt gttggcaaag aagtgttcgc
     7141 cccggtgcag ccatacgggt tgcaaatcag tcagggctgc gccgatggcc gtggtgccca
     7201 cactgcgctc ggacagatcg gtgccaatgc gggtcaccat gtcggcccgc gggtccgagt
     7261 ggtcgatggc accacatgcg tccacaacca cgccatcggc attggtgaga atggcaaagt
     7321 agcgcgtgtc ggcaatggcg cgtgccaggt tctccagcag cggccgcgca ctctgcacca
     7381 gttgcgcatt gcgctcggtg gtatggcgca tttgttgggg cgtcagtacc tcaaacacga
     7441 tgtcccgctg cgggtcatgt cccagggcca tgcagcgctg ccacgagcgt tccacccacg
     7501 gtgagagctg cagcgatgcc ggtgcgcggc gttccaccag cagggtttgc cgcgcatgcg
     7561 cgatggcttc cagccgcatg cttgcgttgg ctgcaggaag gtgcgcaggg gctgtgctca
     7621 tcgtgtctca ccaccgtttt ggttgtgcgt gcattttgac ttacatggca ccccagcggt
     7681 gctgtttcat tttgagaatt tcatacgtca cggtatacct gactagggtt atccctgaag
     7741 gtttctgtgg agttcagtcg aacaatgcct atgtaaattg attcataggc atggcattgc
     7801 ataggttgca ggccgcaccc acaggagaga gacgcatgca aaagatgttg cacatcaacc
     7861 cggataaatg caccggttgt ctgcagtgcg aaatggcctg ttctttcgag aactacggca
     7921 cattcgctac cgccaagtcc cgtatcaagg tgtttgagtt tcaccagacc ggcaagaagg
     7981 tgccctacac ctgcacacaa tgcgatgagg cctggtgcct gcatgcctgc ccggtggagg
     8041 ccatcacggt cgacaaggcc accggcgcca aagtggtgaa cgaagccacc tgcgtgggct
     8101 gcaaggtctg caccattgcc tgcccctttg gcaccatcaa ttacgtgcag gaaaccggca
     8161 aggtgcagaa gtgcgatttg tgcggcggcg accccgcctg cgccaccgca tgcaccaccg
     8221 gcgccatcac tttcatcgac gccaactgga ccggcctggg caaga
//
LOCUS       DAOSJM010000057         8180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8172_length_8180_cov_4.301415, whole genome shotgun sequence.
ACCESSION   DAOSJM010000057 DAOSJM010000000
VERSION     DAOSJM010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8172_length_8180_cov_4.301415"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="PKX82_03225"
     CDS             complement(<1..1807)
                     /locus_tag="PKX82_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOF51290.1"
                     /translation="MRIEKLTTKFQEALGEAQSLALGNDNQYIEPAHVLLAMLRQDDG
                     PKALLQRAGVNLPGLTQAAEAAMKKLPQVQGHEQVQVGRDMVTLLQASEKEAMKRNDQ
                     FVASELFLLALADSKADIGNVVRANGLTRKSLETAIDAVRGGQSVDSADAEDQRESLK
                     KYCIDLTERARQGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVDGAVPDSLKGKRVLSLDMAALLAGAKFRGEFEERLKNVLKDLAKDEGQTIVFID
                     ELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQKI
                     LVGEPTVEATIAILRGLKERYETHHGVQITDPAIVAAAELSNRYITDRFLPDKAIDLI
                     DEAASKIKIELDSKPEVMDKKDRRLIQLQIEREAVKREKDEASQKRLELINEEITALQ
                     KEIADLDEIWKAEKAQAQGSKTIMQEVEKLRFQIKELTDKGDFNKAGELQYGKLPELE
                     KRLKDAQAQEAGKAQSAKDGGRAQLLRTMVGADEIAEVVSRATGIPVSKMMQGERDKL
                     LQMETKLHDCVVGQNEAIAAVADAIRRSRSGLSDPN"
     gene            1901..2470
                     /locus_tag="PKX82_03230"
     CDS             1901..2470
                     /locus_tag="PKX82_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51291.1"
                     /translation="MDIHQLQATYQQEQDRILLRLNTRTQEEFRLWLTRRMVKNFYPH
                     LVEVSKRMGGDAAHHVSHDGGLADDLAGFQQQESLATADFETPFSGETTALPAGSEPL
                     LVTQMKISQLDDHNLHLALEEKMADGSTTRAFEVTLGLPIIHALLHLLDLVIKSADWG
                     LTPVPGVPASEPSPGMDAFETAERPQYLN"
     gene            complement(2467..2964)
                     /locus_tag="PKX82_03235"
     CDS             complement(2467..2964)
                     /locus_tag="PKX82_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HOF51292.1"
                     /translation="MSHTAPRVSIQTEDFDLGAEIATLRAQDGRVGAVCSFVGTVRDR
                     TAGIAGQIQSMELEHYPGMTEKSIEAMIDAAHARFDIFGARVIHRVGLLQPLDQIVMV
                     AVTSAHRGESFAACEFLMDYLKTQAPFWKKEQTPEGAKWVDARVSDDAALAKWGIAAS
                     NGNAA"
     gene            complement(2970..3221)
                     /locus_tag="PKX82_03240"
     CDS             complement(2970..3221)
                     /locus_tag="PKX82_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOF51293.1"
                     /translation="MKVSIKYFASIREAIGQPGEVRETQATTLAALRDELIASSDTYA
                     QCLARGRAVRMALNQVMSEESAALIDGAEVAFFPPVTGG"
     gene            complement(3218..4462)
                     /locus_tag="PKX82_03245"
     CDS             complement(3218..4462)
                     /locus_tag="PKX82_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOF51294.1"
                     /translation="MATAPRQPLLALDDALTQLLGAVTPLQDTEQVATLQADGRVLAA
                     DVVSGLHVPPQDNSSMDGYAVRCADVAAAGATLTVSQRIAAGAAGTELLPGTAARIFT
                     GAPIPPGADAVVMQEDCTAADGAQGQPCVTVNSVPVSGQWIRRAGEDVTRGAVVLARG
                     QRLGPAALGLAASVGMAELSVVRRPRVALFSTGDELVMPGQKAPQDMPPGAIYNSNRF
                     FLAALLRRLGCEVSDLGIVPDQREATVEALRSAAANHDVILTSGGVSVGEEDHIKPAV
                     QSLGSLDLWQIAIKPGKPFAHGRVGNAHFLGLPGNPVSSFVTFLVLVRPFLLQMQGVS
                     DVAMKSIAVRADFTWARADKRREFLRVRRNAQGGLDLFPNQSSGVLTSAVWGDGVIDN
                     PPGRTIAPGDVVQFVPFAEVMA"
     gene            complement(4465..5895)
                     /gene="thrC"
                     /locus_tag="PKX82_03250"
     CDS             complement(4465..5895)
                     /gene="thrC"
                     /locus_tag="PKX82_03250"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOF51295.1"
                     /translation="MKYLSTRGHTDRKRFCEILLEGLAPDGGLYMPESYPQVSDATLT
                     RWRDVYHRQGYAELAFEILSLYIDDIPAADLRALCEKTYTAAVYGTGEIVPLRHVEDG
                     LWIEALSNGPTLAFKDMAMQLLGNLFEYELARRGEELNILGATSGDTGSAAEYAMRGK
                     KGVRVFMTSPFGRMSPFQQAQMFSLMDANIYNIAVKGVFDDCQDMVKAVSNDLEFKRK
                     YKIGTVNSINWARLLAQVVYYFAGYIQATESNAQKVSFSVPSGNFGNVCAGHVARMMG
                     LPIEKLVVATNENDVLDEFFRTGVYRVRGTADTHETSSPSMDISKASNFERFVFDLLG
                     RDGDRVKALFGDALNTAGRFDLSADPAFAQAASRYGFVSGKSTHAHRLETIRDTWKRH
                     EIMIDTHTADGLKVALEHRTPGVPMIVLETALPIKFAATIVEALGREPERPAKFEGIE
                     ALPKRVIVMEADVAAIKALIARECAY"
     gene            complement(5895..7217)
                     /locus_tag="PKX82_03255"
     CDS             complement(5895..7217)
                     /locus_tag="PKX82_03255"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOF51296.1"
                     /translation="MKPIQVGLLGIGVVGSGTFNVLKRNQQEIQRRAGRGIEITMVAD
                     LDTARAQAAVGPDVKVVNDARAVIANPDIDIVIELIGGYGIAKTLVMEAIAAGKHVVT
                     ANKALLAVHGTEIFAAASAKGVMVAFEAAVAGGIPIIKALREGLTANRIQWIAGIING
                     TTNFILSEMRDKGLDFGVVLKEAQRLGYAEADPTFDIEGVDAAHKATIMSAIAFGIPV
                     QFDKAYVEGITKLGAADIKYAEQLGYRIKLLGIAKRRPEGIELRVHPSLVPSKRLIAN
                     VEGAMNAVMVQGDAVGTTLYYGKGAGSEPTASAVIADLVDIARLHTADPQNRVPHLAF
                     QPDALSDLKVLPMEQVVTSYYLRLRVADQAGVLAKVTGILAESGISIDAVLQREADEV
                     GGEGSTQTDVIILTHDCVEAKMNAAIAQMQALPTVLAPITRIRKEELA"
     gene            complement(7296..>8180)
                     /locus_tag="PKX82_03260"
     CDS             complement(7296..>8180)
                     /locus_tag="PKX82_03260"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOF51297.1"
                     /translation="LLDDGDELLLPAPDYPLWTAAVSLSGGTPVHYMCDEANGWMPNL
                     HDIRSKITPRTKGIVVINPNNPTGAMYSDELLLAIVAIAREHGLVIFADEVYDKVLYD
                     GVKHTAIGSLSEDVLTLTFNSLSKSYRSCGYRAGWMIISGDKRPAKDYIEGLNMLSNM
                     RLCSNVQGQYAIQTALGGYQSINELVGEGGRLRRQRDLAYELITAIPGVTCVKPTAAL
                     YMFPRLDPAMYPIEDDQQFFLELLQETRVMLVQGTGFNWPEPDHFRIVFLPHEDDLRE
                     AIGRVAKFLEHYRKRHSN"
BASE COUNT         1440 a         2586 c         2525 g         1629 t
ORIGIN      
        1 gattcgggtc ggagaggccc gagcgcgaac ggcgaatggc atcggccacg gccgcaatgg
       61 cctcgttctg gccgaccacg cagtcgtgca gcttggtttc catttgcagc agcttgtcac
      121 gctccccctg catcatcttg gacacgggga tgccggtggc gcggctcacc acctcggcaa
      181 tttcatcggc gccgaccatg gtgcgcagca gttgggcgcg gccaccgtct ttggcgctct
      241 gggccttgcc agcttcctgg gcctgcgcgt ctttcaggcg tttttccagc tcgggcagct
      301 tgccgtattg cagctcgccc gccttgttga agtcgccctt gtcggtgagt tccttgatct
      361 ggaagcgcag cttttccacc tcctgcatga tggtcttgga gccctgggcc tgggccttct
      421 cggccttcca gatttcgtcc agatcggcaa tttccttttg cagcgcggtg atctcttcgt
      481 tgatcagctc caggcgcttt tgcgaggctt cgtccttctc gcgcttcacg gcttcacgct
      541 cgatctgcag ctggatcagg cggcggtctt tcttgtccat cacctcgggc ttggaatcca
      601 gctcgatctt gatcttgctg gcggcttcgt cgatcaggtc gatggcctta tccggcaaaa
      661 agcggtcggt gatgtagcgg ttggacagct cggcagcggc aacgatcgcc gggtcggtga
      721 tctgcacgcc atggtgggtt tcatagcgct ctttcaggcc gcgcagaata gcgatggtgg
      781 cttccactgt cggctcgccc accagaattt tctggaagcg gcgctccagc gcggcgtcct
      841 tctcgatgta cttgcggtac tcgtccagcg tggtggcacc cacgcagtgc agttcgccgc
      901 gggccagcgc gggcttgagc atattgccgg cgtccatggc gccttcggcc ttgccggcgc
      961 ccaccatggt gtgcagctcg tcaatgaaaa caatggtctg gccttcgtcc ttggccaaat
     1021 ccttgagcac gtttttcagg cgctcttcga actcgccacg gaacttggca ccagccagca
     1081 gggcggccat gtccagactc agcacgcgct tgcccttgag ggagtccggc accgcgccgt
     1141 ccacaatgcg ctgggccagg ccttccacaa tcgccgtctt gccgacaccg ggctcgccga
     1201 tcagcaccgg gttgtttttg gtgcggcgct ggagtacctg aatggcgcgg cggatttcgt
     1261 cgtcacggcc aatcaccggg tcgagcttgc cctggcgtgc gcgctcagtc aggtcgatgc
     1321 agtatttctt gagtgactcg cgctggtctt cggcatctgc actgtccacg ctctggccac
     1381 cgcgcaccgc gtcgatcgcg gtttccaggc tcttgcgggt caggccgttg gcgcgcacca
     1441 cgttgccgat atcggctttg ctgtcagcca gcgccagcaa aaagagttcg ctggccacaa
     1501 actggtcgtt gcgtttcatg gcctctttct cagacgcctg caacagcgtc accatgtcac
     1561 ggcccacctg cacctgctcg tggccctgca cctgcggcag cttcttcatg gcggcttctg
     1621 cagcctgtgt caggccgggc aggttgacgc ccgcgcgttg cagcaacgcc ttggggccat
     1681 cgtcctggcg cagcatggcc agcagcacgt gggccggctc gatgtactgg ttgtcgttgc
     1741 ccagggccag cgattgggcc tcgcccagag cttcctggaa tttcgtggtg agtttttcga
     1801 tacgcatggc aaatcccttt gtctagttga accgcaagtt gggacatccc cctctatttc
     1861 aagacaggcg gttttcgggt ctattgacta gaatcaaagc atggacatcc accagttaca
     1921 ggccacctac cagcaggaac aggaccgcat tctgctgcgc ctgaacaccc gcacacagga
     1981 ggaattccgc ctgtggctga cgcgacgcat ggtgaaaaat ttctaccccc atctggtgga
     2041 agtttccaag cgcatgggcg gcgacgccgc acaccacgtc agccacgatg gtggcctggc
     2101 ggacgatctg gctgggtttc agcaacagga atcactggct acagccgatt tcgagacgcc
     2161 atttagcggc gaaaccaccg cactgcccgc gggcagtgag ccgctgctgg ttacgcagat
     2221 gaagatttcc cagctcgacg accacaacct gcacctggcg ctggaagaaa aaatggccga
     2281 tggttccacc acacgggcgt ttgaggtgac attgggcctg cccatcatcc atgccctgct
     2341 gcatttgcta gacctggtga tcaagagcgc cgactggggc ctgacgccgg tacccggcgt
     2401 gcctgctagc gagccgtcac ccggtatgga tgcgtttgag accgccgagc ggccgcagta
     2461 tctgaattaa gcggcgttgc cgttggaggc cgcaatgccc cacttggcca gcgccgcgtc
     2521 gtcgctaacc cgggcatcca cccacttggc gccctcggga gtttgctctt tcttccagaa
     2581 cggcgcctgg gtcttcaggt agtccatcag gaactcgcac gcggcaaaac tctcgccgcg
     2641 gtgggccgaa gtgacggcca ccatcacgat ctggtccagc ggctgcaaca ggcctacgcg
     2701 atgaatcacc cgtgcgccaa agatgtcgaa ccgggcatgg gccgcgtcga tcatggcttc
     2761 gatcgatttc tcggtcatgc cgggatagtg ctccagctcc atgctctgga tctggcccgc
     2821 aatgcctgca gtgcggtcgc gtacggtgcc gacaaaactg cacaccgcgc ctacgcggcc
     2881 atcctgcgcc cgcagggtgg cgatctcggc accgaggtca aagtcttcgg tctggatact
     2941 gacccggggc gcggtatggg acatggcgat cagcctccag tcactggagg aaagaatgcg
     3001 acttctgcac catcgatgag ggcagcagac tcctcgctca tcacctggtt cagcgccatg
     3061 cgcaccgcac ggccacgggc caggcactgc gcataggtgt cgctggaagc gatgagttcg
     3121 tcgcgcagcg cagccagcgt agtggcctgc gtctcgcgca cttcaccggg ctggccaatg
     3181 gcctcgcgga tggaggcgaa atacttgatg gaaaccttca tgccatgact tccgcaaacg
     3241 gaacaaactg caccacatcg cccggggcaa tggtgcgccc gggagggttg tcgataacgc
     3301 catcgcccca gacggcggag gtcagcacgc ccgagctctg gttgggaaac agatccagcc
     3361 caccctgggc attgcggcgc acacgcagga attcacggcg tttatcggct ctggcccagg
     3421 tgaaatctgc gcgaacagct atggatttca tagcaacatc agacacaccc tgcatctgca
     3481 gcagaaatgg ccgcaccagc accagaaagg tcacaaagct cgacaccgga ttgcccggca
     3541 aacccagaaa gtgcgcattg cccacccggc cgtgtgcaaa cggtttgcct ggcttgatgg
     3601 caatctgcca caggtccagc gaacccaggg actgcacggc gggcttgatg tggtcttcct
     3661 cccccaccga cacgccaccg ctggtcagga tcacatcgtg gtttgcagcc gcgctgcgca
     3721 atgcttccac cgtggcttcg cgctggtccg gcacgatacc caggtcggac acctcacagc
     3781 ccaggcggcg cagcagcgct gccaggaaga agcggttgga gttgtagatg gcaccgggtg
     3841 gcatgtcctg cggcgctttc tggcccggca tcaccaactc atcgccggtc gaaaacaagg
     3901 ccacgcgcgg acggcggacc acgctgagtt cggccatgcc cacgctggcg gccaacccca
     3961 gtgcggcggg gcccaggcgc tggccacgcg ccagcactac ggcaccgcgg gtgacgtctt
     4021 cacccgcacg gcgaatccac tgcccggaga cgggcacgct gttgacggtc acgcacggct
     4081 ggccctgcgc accgtctgcg gcggtgcagt cttcctgcat gaccacggcg tcggcaccgg
     4141 gcggaatcgg ggctccggta aagatgcgcg cagcggttcc ggggagcagc tcggttccgg
     4201 ccgcgccggc agcaatgcgc tgggacacgg tcagcgtggc accggcagcg gccacatcgg
     4261 cgcaacgcac cgcgtagccg tccatcgagc tgttgtcctg cggtggcaca tgcaggcccg
     4321 aaaccacatc ggccgccagc acacggccat cggcctgcag cgtggcaacc tgctcggtgt
     4381 cctgcaaggg tgttaccgcc cccagcagtt gggtcagcgc atcgtcgagc gccagcaaag
     4441 gctgtcgggg tgccgtggcc atggctagta ggcgcattcc cgcgcgatga gggccttgat
     4501 ggcggccaca tcggcttcca tcacgatcac gcgcttgggc agggcttcaa tgccttcgaa
     4561 cttggcgggc cgctcgggct cgcgccccag tgcttccacg atggtggccg caaacttgat
     4621 gggcaaagcg gtttccagca cgatcatggg cacgccgggg gtccggtgtt ccagcgccac
     4681 cttcaggccg tcggccgtgt gggtgtcgat catgatttcg tggcgcttcc aggtatcacg
     4741 gatggtttcc agacggtggg catgcgtgct cttgccgctg acaaacccgt aacggctggc
     4801 tgcctgcgca aacgcggggt ccgcgctcag gtcaaagcgg ccggccgtgt tgagcgcgtc
     4861 gccaaacagc gccttgacgc gatcaccgtc gcgtcccagc aggtcgaaca caaagcgctc
     4921 gaagttgctg gccttggaaa tgtccatgga cgggctggag gtctcgtgcg tgtcggcggt
     4981 gccacgcacg cggtacacac cggtgcggaa gaactcgtcg agcacatcgt tttcgttggt
     5041 ggctaccacc agcttctcga tcggcaggcc catcatgcgc gccacatggc ctgcgcagac
     5101 gttgccaaag ttgccgctgg gcacgctgaa agacaccttc tgtgcgttgg actcggtggc
     5161 ctggatgtag ccggcaaagt agtacaccac ctgcgccaac aggcgcgccc agttgatgga
     5221 attcacggtg ccgatcttgt atttgcgttt gaattcgagg tcgttgctga ccgccttgac
     5281 catgtcctgg cagtcatcga acacgccctt gaccgcgatg ttgtagatgt tggcgtccat
     5341 caggctgaac atctgcgcct gctggaacgg gctcatacgc ccgaacgggc tggtcatgaa
     5401 gacgcgcacg ccctttttgc cgcgcatggc gtattcagcc gcgctaccgg tgtcgccact
     5461 ggtggcgccc aggatgttga gctcttcgcc gcggcgtgcc agttcgtatt cgaacaggtt
     5521 gcccagaagt tgcatcgcca tgtccttgaa ggccagtgtg gggccgttgg acaacgcctc
     5581 aatccacagg ccgtcttcga cgtggcgcag gggaacaatc tcgccggtgc catacaccgc
     5641 agcggtgtag gtcttctcgc acagcgcgcg caggtcggcg gcgggaatgt cgtcaatgta
     5701 gagcgacaga atctcgaaag ccagctccgc atagccctgg cggtggtaca catcgcgcca
     5761 gcgcgtcaac gtggcgtcgc tcacctgcgg gtaggactca ggcatgtaca gcccgccatc
     5821 gggcgccagg ccctcgagca ggatttcgca gaagcgtttg cggtccgtgt ggccgcgtgt
     5881 actcaggtac ttcatcaggc gagttcttcc ttgcggatac gggtgatcgg ggccagcacg
     5941 gtgggcagtg cctgcatctg ggcaatggcg gcgttcatct tggcttccac gcagtcgtgg
     6001 gtcaggatga tgacgtcagt ctgggtggag ccttcaccgc ccacttcgtc cgcttcgcgc
     6061 tgcaacaccg cgtcgatgct gataccgctc tcggccagaa tgccggtcac tttggccagc
     6121 acgccagcct ggtcggccac gcgcaggcgc aggtagtagc tggtgaccac ctgctccatg
     6181 ggcagcactt tcaggtcgct cagtgcatcg ggctggaacg ccaggtgcgg cacacggttt
     6241 tgcgggtcgg cggtgtgcaa acgggcaatg tccaccagat cggcaatcac cgcgctggcg
     6301 gtgggctcgg aaccggcacc cttgccgtag tacagcgtgg tgcccacggc atcgccctgc
     6361 accatgaccg cattcatcgc accttccacg ttggcaatca gacgcttgct cggcaccagg
     6421 ctggggtgca cgcgcaactc gatgccttca gggcggcgtt tggcaatgcc cagcagcttg
     6481 atgcggtagc cgagttgttc ggcgtacttg atatcggcag cgcccagctt ggtgatgccc
     6541 tccacatagg ccttgtcaaa ctgcaccgga atgccaaacg caatagccga catgatggtg
     6601 gccttgtgcg cagcatccac tccttcgatg tcaaatgtgg ggtcagcttc cgcataaccc
     6661 aggcgctgcg cttccttgag caccacgcca aagtccaggc ccttgtcccg catttcggac
     6721 aggatgaaat tggtggtgcc attgatgatg ccggcgatcc actggatgcg gttggcggtc
     6781 aggccttcac gcagcgcctt gatgatggga atgccaccag ccactgccgc ttcaaacgcc
     6841 accatcacgc ccttggccga ggcggccgcg aagatttcgg tgccgtgcac ggccagcaag
     6901 gctttgttgg cggtgaccac atgcttgccg gcggcaatcg cttccatcac cagcgttttg
     6961 gcgataccgt agccaccgat cagttcaatg acgatgtcga tgtcggggtt ggcaatgacg
     7021 gcgcgggcgt cgttgaccac cttcacatcg gggccgacgg cggcctgcgc gcgtgcggta
     7081 tcgaggtcgg cgaccatggt gatttcaatg ccacggccag cgcggcgctg gatctcctgc
     7141 tggttgcgct tgagcacatt gaatgtgccg gaacccacta cgccaatgcc caacaggccc
     7201 acttgaatcg gtttcataca gtctctacag tttcagttaa atcagctcat agcccccgta
     7261 gaatgtgtgc gggtagctat caaatacata gcaaactagt tgctgtgccg tttgcggtaa
     7321 tgctccagga acttggccac gcggccaatg gcctcgcgca ggtcgtcttc atggggcagg
     7381 aacacaatgc ggaaatggtc cggctcgggc cagttgaagc ccgttccctg caccagcatc
     7441 acacgtgttt cctgcagcag ttccaggaag aactgctggt cgtcttcaat cgggtacatg
     7501 gccgggtcca gccgcgggaa catgtacagc gcagcggtgg gcttcacgca ggtcaccccg
     7561 ggaatggccg tgatcagctc atacgccagg tcgcgctggc ggcgcaaacg gccgccctcg
     7621 cctaccagtt cgttgatgct ctggtagccg cccagtgccg tctgaatcgc atactggccc
     7681 tgcacgttgg agcacaggcg catgttcgac agcatgttga ggccttcgat gtaatccttg
     7741 gctggacgct tgtcgccgga gatgatcatc cagcccgcgc ggtagccgca ggagcggtag
     7801 ctcttggaca gggagttgaa ggtcagcgtc agcacgtctt cgctgagcga gccaatagcg
     7861 gtgtgtttga cgccgtcgta cagcaccttg tcgtacacct cgtcggcaaa aatcaccagg
     7921 ccgtgctcac gtgcaatcgc cacaatcgcc agcagcaatt cgtccgaata catggcaccg
     7981 gtcgggttgt tggggttgat caccacaatg cccttggtgc gcggcgtgat cttgctgcgg
     8041 atgtcatgca gattgggcat ccagccattg gcttcgtcgc acatgtagtg caccggcgtg
     8101 ccgcccgaca ggctgactgc agccgtccac agggggtagt caggcgctgg caacagcaat
     8161 tcgtcgccgt cgtcgagcag
//
LOCUS       DAOSJM010000058         8179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8173_length_8179_cov_4.460980, whole genome shotgun sequence.
ACCESSION   DAOSJM010000058 DAOSJM010000000
VERSION     DAOSJM010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8179
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8173_length_8179_cov_4.460980"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1259)
                     /locus_tag="PKX82_03265"
     CDS             complement(42..1259)
                     /locus_tag="PKX82_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF51298.1"
                     /translation="MTPRPMLARLGPFLLSCLLAFTAGHMFNYTVILYLQEAVGSDLL
                     SGLGFGLAFGSSIVFGWFGGVLCDRVAPHRVIHGAQALFLAGLACLWWAQSGAAPEVR
                     VAWVLGGAFLGGLAWSFVGPARLTTLAQMARPEELRPATILFNLQVLVGFGLAPLLIG
                     IVRSQSGWGAVIALGACGFGLSSLLIANLRTHANPTVSSNSVLADILEGFRTVGKDPL
                     LTQLMLAAVLGYAMTGPLQILLPKLAREVLYLSELQRGAYLGLLALSLIVGGVTALAV
                     AKRVRHGLVMFIGIALGGLVFASLSIIGAVGLSAAALASVGMAGGMVISLVVSGIQAQ
                     APVALRGRVMGMYSITSQVVPALSGVAAGALVNAVGVVTAIQVSGLTLAALVALAVWR
                     MGALRRYTGQATG"
     gene            complement(1256..3049)
                     /locus_tag="PKX82_03270"
     CDS             complement(1256..3049)
                     /locus_tag="PKX82_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51299.1"
                     /translation="MKQHTNLLGALIALATLSTGALAADFQVQGPDGKPLPLVMVTRA
                     PSQPAVTDTSDNGYAPTGKLQQGTFEFTGFTDLGGRVHLPDAPGDYRVRLRKPGFKDH
                     WIEPALLAKATSWRMEAETDLKALAEQRPSNAWTSTLLAGRDDLRKEFMAQCGFCHQQ
                     GSSFLRRERSAEDWDSTIKRMVRYGARLSTEAQKELPALLEAHWKDIAAHPEKVPAGT
                     PWSPALSAASVTELPIGDRFSQMHDFIQHSNGMVYVGDNLQDRLYEVNPQTGAYTVYR
                     VPPQPGDNLGGLLAGRLKDFPKHETYQGIHSLAESDRDGHIFITPSYQRRLIEFDPKT
                     KTFSNHEIGSGFYPHTVRIDDKDRVWFTLALSNQIGMYDRTANKFTTYDLPFRSFMEK
                     LTVKLTPFFFKLIGWGVPVTNWVKIDHVSTGVPLPYGIDITPDGKVWIARLHTDEIAS
                     LDPATGEITMFATPLKNPRRLRTDRDGNLWIGMFSESAVLRFEPKTAKFTTFHLPVQP
                     VGSDTPYALNVDRKRHQLWVNGTNSDGVHRLDIATGQWTHFPLPRKATFTRDVEFTPN
                     GQVLLSSASFPSWHIEDAQPTLIRLNPNARK"
     gene            complement(3068..3895)
                     /locus_tag="PKX82_03275"
     CDS             complement(3068..3895)
                     /locus_tag="PKX82_03275"
                     /inference="COORDINATES: protein motif:HMM:NF004825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF51300.1"
                     /translation="MRCFAGRGVVITGAAGGLGAALVQSFLKEGAQVLALDRDAQALQ
                     ALQQAYPTTDAEGAPRLLTQVCDVTDAAACAAAMAFATQRLGGVDVLVNNAGIAHRSA
                     FANTRLDVLRRVMDVNFFGAVHCTQAALESLRSRHGLIITVSSVAGFAPLIGRTGYAA
                     SKHALHGFFDSLRTELEDDGVGVLLVCPSFIDTGIDQAALGGDGAPAAQGRKTTGGQT
                     QPAEVAHAILAAAHRSRRQLLMGSTARAAWWLSRLWPSRYARIMKLRLQAEIFPSNT"
     gene            complement(3897..4862)
                     /locus_tag="PKX82_03280"
     CDS             complement(3897..4862)
                     /locus_tag="PKX82_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF51301.1"
                     /translation="MSRVLITGGGGYLGSQLVARLAALPEGLRPSAIVSHDIREPKVH
                     LSGVGYAVADIRSVEIVNFIANHQIDTVVHLASIVTPGKDSNRAFEYDVDVNGTRNVL
                     EACVHNGVKRIVVSSSGAAYGYHADNAPWLTEDMPVRGNEVFAYSHHKRLVEEMLAEY
                     RQKYPQLEQVVLRIGTILGPTVHNQITDMFEKPRLLTIRGSDSPFVFIWDQDVVGIIA
                     RAISDGPAGIYNVAGDGALTIHEIAQRLGKKTLNLPAWLLQTALRILKPLGLTQYGPE
                     QLDFLRYRPVLLNTKLKQEFGYTPTYTSAQVFEIYREARERAGAA"
     gene            complement(4859..5788)
                     /locus_tag="PKX82_03285"
     CDS             complement(4859..5788)
                     /locus_tag="PKX82_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOF51302.1"
                     /translation="MAPIDQVHLSFNPQTLTLLNAVLGLVMFGVALELRVEDFKVALR
                     TPKALALGLFGHHILFPAMTYVLIWILQPVPSIALGMILVSSCPAGHISNFLVHYSKG
                     NTALSVSVSALSTVAALFMTPLNFAFWGNLHPVTSGLMKEVAMSPWEMLEVIVMLLGV
                     PLVLGMWVARQYPGFAHKAQKPMKVLSLIILIAFIGGALGANFRHFMDYISLVLGVVF
                     IHNLMALVTGYGLSAAIGLPERDRRAITFEMGIQNSGLGLILIFNFFDGLGGMAIVTA
                     WWGVWHIVAGMTLATFWKGRPPKDDTSAAKEQA"
     gene            5872..6384
                     /locus_tag="PKX82_03290"
     CDS             5872..6384
                     /locus_tag="PKX82_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOF51303.1"
                     /translation="MGQPDHYSVTPRFTQAILEAAQRHGVRVPDALRVALTQSVRVPM
                     SQQDALWTLLASATDDPLTALRVGTDLQAGHLDIVGMLLLTCDTLGDALEVLTEYAPI
                     VGDNARFEVAPEGAGVVLRYLPEYRVCLDERVEAALGCVVCLARWMTNGRFTAREVLF
                     THAPRADAAA"
     gene            6406..6864
                     /locus_tag="PKX82_03295"
     CDS             6406..6864
                     /locus_tag="PKX82_03295"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOF51304.1"
                     /translation="MQFGQPFNGVVLDADALGLGLIQAGKTMHAHLKVLADDMLASLP
                     QGSVSAQVRQLIREHPRWGKERIGEQLGMSGRNLNRKLAAESISFKGLREALIYDMAS
                     AALRAGQSVAAVSEKLGFSDENAFSRAFRRWSGLTPAQFVRGGDATLAPE"
     gene            6879..7652
                     /gene="yaaA"
                     /locus_tag="PKX82_03300"
     CDS             6879..7652
                     /gene="yaaA"
                     /locus_tag="PKX82_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOF51305.1"
                     /translation="MLFLLSPAKSLDYETPLGDQPHTLPMFVPQSKELIAVLRTQSPQ
                     QIAELMSLSDKLSALNVARYLAWSPKATPKNARQAVLAFNGDVYDGLDAKTLTPDDLA
                     WAQDHVCILSGLYGVLRPLDLMQPYRLEMGTRLSNPGGKDLYQFWGTQIADYLNTRLR
                     SDVSPVVINLASQEYFKAVDTKALKARVVECVFQDYSAGQYKIVSFFAKRARGLMARY
                     AATHRLVQPEQLRAFDLDGYAWAAAESTSERLVFRRRAA"
     gene            7702..>8179
                     /locus_tag="PKX82_03305"
     CDS             7702..>8179
                     /locus_tag="PKX82_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF51306.1"
                     /translation="MKIRANGIDIEVEDSAQSHPETADKPVVLLIMGLGMQLIAWPPQ
                     MLQGLVDAGYRVVRFDNRDVGLSSHFDQLGKPNLLWASLKYKIGLVPAAPYTLSDMAA
                     DALGVLDALGIAKAHVVGVSMGGMIAQRVAIAAPQRTLSLTSIMSSSGARGLPQADP"
BASE COUNT         1490 a         2655 c         2612 g         1422 t
ORIGIN      
        1 gcgcaatgga tacgggggct ggaggccgat ttggcttgca atcaccccgt tgcctgcccg
       61 gtgtagcgcc gcagcgcccc catgcgccac acggccaggg ccaccagtgc cgccagggtc
      121 aggcccgaca cctgaatcgc tgtcaccacg cccaccgcat tgaccagtgc gccggccgcc
      181 acacccgaca gagccggtac cacctgcgag gtgatggagt acatgcccat gacccggccc
      241 cgcaaggcca ccggcgcctg ggcctggatg ccagagacga ccaggctgat caccatgccg
      301 ccggccatgc ccacgctggc cagcgcagca gccgacagac ccaccgcacc gatgatgctc
      361 agcgatgcaa acaccagacc accgagcgca atgccaatga acatcaccag cccgtggcgc
      421 acgcgtttgg cgactgcgag ggcggtgacg ccgcccacga tcagcgagag cgccagcagc
      481 cccagatacg ccccgcgctg cagttccgag aggtacagta cttcccgcgc cagcttgggc
      541 agcaggatct gcagcgggcc ggtcatggca tagcccaaca cggcggccag catcagctgc
      601 gtcagcagcg ggtctttgcc gacggtgcgg aagccctcca aaatatcggc cagcaccgag
      661 ttgctgctga cggtggggtt ggcgtgggtg cgcaggttcg cgatcagcaa agacgacagc
      721 ccaaagccgc atgcgcccag cgcaatcacc gcgccccagc cgctctggct gcgtacgatc
      781 ccgatgagca acggcgccag cccaaatccc accagcacct gcaaattgaa gaggatggtg
      841 gctggccgca attcttccgg ccgcgccatt tgcgccagcg tggtcagccg cgccggcccg
      901 acgaacgacc aggccagacc gcccagaaac gcgccgccca gcacccaggc cacgcgcact
      961 tccggcgcag caccggattg cgcccaccac aggcacgcca gcccagccag aaacagggcc
     1021 tgtgcgccgt ggatcacccg gtgcggggct acgcggtcgc atagcacgcc gccaaaccag
     1081 ccaaacacga tggagctgcc aaacgccagt ccgaagccca gacccgagag caggtccgac
     1141 cccaccgcct cctgcaggta caggatcacg gtgtagttga acatgtgccc ggcggtaaac
     1201 gccagcaggc acgacagcag aaaaggcccc agccgggcca gcatcgggcg cggtgtcact
     1261 tgcgggcgtt cgggttgagg cggatcagcg tgggctgcgc atcttcaatg tgccaggacg
     1321 ggaagctggc gctggagagc agtacctggc cgttgggtgt gaattccacg tccctggtga
     1381 aggtggcctt gcgcggcagc ggaaagtggg tccactggcc ggtggcaatg tcgagccggt
     1441 gcacgccgtc cgagttggtg ccgttgaccc acagctggtg gcgcttgcgg tccacgttca
     1501 gcgcgtacgg ggtgtcgctg cccacgggtt gcaccggcag gtggaacgtg gtgaatttgg
     1561 cggtcttcgg ctcgaagcgc agcacggccg actcgctgaa cataccgatc cacagattgc
     1621 catcgcggtc ggtgcgcagg cggcgcggat ttttcagcgg cgtggcaaac atggtgatct
     1681 cgccggtggc gggatcgagc gacgcaattt catcggtatg caggcgcgca atccagacct
     1741 tgccatcggg cgtgatgtcg atgccatacg gcaacggcac gccggtcgat acgtggtcaa
     1801 tcttcaccca gttggtgacc ggaacccccc agccaatcag cttgaaaaag aagggcgtga
     1861 gcttgaccgt gagcttttcc atgaacgagc ggaacggcag gtcgtaggtg gtgaacttgt
     1921 tcgcagtgcg gtcatacatg ccgatctggt tggagagggc cagcgtgaac cacacgcggt
     1981 ccttgtcgtc gatgcgcaca gtgtgcgggt agaagccgct gccaatctcg tggttgctga
     2041 aagtcttggt cttgggatcg aactcgatca ggcggcgctg gtaggacggt gtgatgaaga
     2101 tgtgtccgtc cctgtcagac tccgccagcg agtggatgcc ctggtaggtc tcgtgcttgg
     2161 gaaaatcctt caggcgtccg gccagcagac cacccaggtt gtcgcccggt tgcggtggca
     2221 cgcggtacac cgtgtaggcg cccgtttggg ggttgacctc gtacaggcgg tcctgcaggt
     2281 tgtcgcccac gtagaccatg ccattgctgt gctggatgaa gtcgtgcatc tgcgaaaacc
     2341 ggtcgccaat cggcagttcg gtcacgctgg cagccgacag tgccgggctc cacggtgttc
     2401 cggcgggcac tttctccggg tgggcggcga tgtctttcca gtgtgcttcc aacagggccg
     2461 ggagttcctt ttgggcttcg gtagacaggc gtgcgccgta gcgcaccatc cgcttgatcg
     2521 tggaatccca gtcttcggca ctgcgttcgc gccgcaggaa gctgctgccc tgctggtggc
     2581 agaagccgca ctgcgccatg aactccttgc gcaggtcatc gcgcccggct agcagggtcg
     2641 atgtccaggc gttcgagggg cgttgttcgg ccagggcctt gaggtcagtc tccgcctcca
     2701 tgcgccagga tgttgccttg gccagcaaag ccggttcaat ccagtggtcc ttgaaccccg
     2761 gcttgcgcag gcgcacacgg tagtcgccgg gtgcatcggg cagatgcacg cgtccgccca
     2821 aatcggtaaa acccgtgaat tcaaaggtgc cctgctgcag cttgccggtg ggggcatagc
     2881 cgttatccga agtgtccgtc acggcaggct gtgagggggc gcgggtcacc atgaccagcg
     2941 gcaacggctt gccgtccggc ccctgcacct gaaagtcggc cgccagcgcg cctgtggaca
     3001 gcgttgccag tgcgatcagc gctcccagca ggttcgtgtg ttgtttcatg caagtctccg
     3061 ttgcttctta cgtgttcgat ggaaaaattt cggcctgcag gcgcagcttc atgatccggg
     3121 catagcggga gggccagagt cgggacagcc accacgccgc gcgcgccgtg ctgcccatca
     3181 ggagctggcg ccggctgcgg tgtgcagcgg ccaggatggc gtgggccact tcggcgggct
     3241 gggtctggcc accggtggtc ttgcggccct gcgcagccgg tgcaccgtcg ccgcccaggg
     3301 cagcttggtc gataccggtg tcaatgaaag aagggcacac cagcagcacg cccacgccgt
     3361 cgtcttccag ttcggtgcgc aggctgtcaa agaagccatg cagcgcgtgc ttgctggctg
     3421 cgtatcctgt gcgcccgatc agcggcgcaa atcctgccac gctggaaacg gtgatgatga
     3481 ggccatgccg gctgcgcaga ctctccagcg ccgcctgggt gcaatgcacg gcaccaaaaa
     3541 agttgacgtc catgacacgg cgcagcacat ccagccgggt atttgcaaat gcactgcgat
     3601 gggcaatgcc ggcgttgttg accagcacat ccacgccccc aaggcgttgc gtcgcgaacg
     3661 ccatggctgc agcacacgcg gctgcatcgg tgacatcgca gacctgggtc agcagccggg
     3721 gtgcgccttc tgcatccgta gtggggtagg cctgctgcag cgcttgcagt gcctgtgcat
     3781 cgcggtccag cgccagtacc tgcgcgcctt ctttcaggaa cgactgcacc agcgcagccc
     3841 ccaggccacc ggccgcgccg gtgatgacca caccgcgtcc cgcaaaacag cgcatgtcag
     3901 gccgctccgg cgcgctcccg cgcttcgcgg taaatctcga agacctgggc cgaggtgtag
     3961 gtgggcgtgt aaccaaattc ctgcttgagc ttggtattga gcagcaccgg gcggtagcgc
     4021 agaaaatcca gctgctccgg cccgtactgg gtcagcccca gcggtttgag gatgcgcagt
     4081 gccgtctgca gcagccaggc gggcaggttc agcgtcttct tgcccagacg ctgcgcaatc
     4141 tcgtggatgg tgagtgcgcc gtcacccgcc acgttgtaga tgcccgcagg gccatcgctg
     4201 atggcgcgcg caatgatgcc caccacgtcc tggtcccaga tgaacacaaa cggactgtcc
     4261 gacccgcgga tggtgagcag ccggggcttc tcgaacatgt cggtaatctg gttgtgcacc
     4321 gtgggcccaa gaatggtgcc aatgcgcagc accacctgct ccagctgggg gtacttctgg
     4381 cggtactcgg ccagcatctc ttccaccagc cgtttgtggt gggaatacgc aaacacctcg
     4441 ttgccgcgca ccggcatgtc ctcggtgagc cagggcgcgt tgtccgcgtg gtagccatag
     4501 gccgcgccgc tgctggacac cacgatgcgc ttgacgccgt tgtgcacgca ggcttcgagc
     4561 acattgcgcg tgccattcac gtccacgtca tactcaaacg cccggttgct gtccttccca
     4621 ggcgtgacga tggacgccag gtgaaccacc gtgtcgatct ggtggttggc gatgaagttg
     4681 acgatttcca cgctacggat gtctgccacc gcatagccca caccggagag gtgaactttg
     4741 ggttcgcgga tgtcatggga gacgattgcc gatgggcgca ggccctctgg cagcgccgcc
     4801 agacgggcta ccaattggct gccgaggtag ccgccaccgc cggtaatgag tacgcggctc
     4861 atgcctgttc ctttgccgcg ctggtgtcgt ctttgggggg gcggcctttc cagaacgtgg
     4921 ccagcgtcat gcccgccacg atgtgccaca cgccccacca ggcggtcacg atggccatgc
     4981 cacccaggcc gtcaaagaaa ttgaagatca ggatcaggcc caggcccgag ttctggatgc
     5041 ccatctcgaa ggtgatggcg cggcggtcgc gctcgggcag gccgatggct gccgacagac
     5101 cgtagcccgt caccagcgcc atcaggttgt ggatgaacac cacgcccagc accagcgaga
     5161 tgtagtccat gaagtgccgg aagttggccc ccagcgcacc gccaatgaag gcgatcagaa
     5221 tgatcagtga cagcaccttc atcggctttt gcgccttgtg cgcaaagccg gggtactgcc
     5281 gcgccaccca catgcccaac accagcggca caccgagcag catcacgatc acctccagca
     5341 tttcccaggg gctcatcgcc acttctttca tcaggccgga agtgacggga tgcaggttgc
     5401 cccagaacgc aaagttcagc ggcgtcatga acagcgcggc caccgtggac agcgcactca
     5461 cactgaccga cagggccgta ttgcccttcg agtagtgcac cagaaagttg gaaatatgcc
     5521 cggcagggca gctggacacc agaatcatcc ccagggcaat gctgggcacc ggttgcagga
     5581 tccagatcag cacataggtc atggccggaa acaggatgtg gtggccaaac aggcccagcg
     5641 ccaatgcctt gggcgttctg agtgccacct tgaaatcttc cacgcgcagc tccagcgcga
     5701 cgccaaacat caccagcccg agcacggcat tgagcagggt cagggtttgc gggttaaagc
     5761 tcaggtggac ctggtcgata ggtgccatgg ttgtgtctcc tcgttatctg ccggacttta
     5821 gggcaaactc cagttttcgt cgctaacttt gcgggacagc agggcatcaa tttgggacag
     5881 ccagaccact acagcgttac cccccggttc acccaggcca ttctcgaagc cgcgcaacgc
     5941 catggcgtgc gggtgccgga tgcgctgcgc gtggccctga cgcaaagcgt gcgggtgccc
     6001 atgtcccagc aggatgcctt gtggacgctg ctggccagtg ccaccgacga cccgctcacg
     6061 gcactgcgcg taggtaccga tctgcaggcc gggcacctgg acattgtggg catgctgctg
     6121 ctcacctgcg acaccctggg cgatgccctg gaagtgctga ccgaatacgc gcccatcgtg
     6181 ggtgacaacg cccgctttga agtcgcgccc gagggcgctg gcgtggtgtt gcgctacctg
     6241 cctgaatacc gcgtgtgtct ggatgagcgg gtggaagccg cgctgggttg cgtggtctgt
     6301 ctggcccgct ggatgaccaa tggccgcttc accgcgcgcg aagtgctgtt tacccatgcg
     6361 ccgcgggccg atgccgctgc gtaagcggcc ttgctgggct gcccggtgca atttggtcag
     6421 cccttcaatg gggtggtcct ggatgccgat gccctgggtt tggggctgat ccaggcgggc
     6481 aagaccatgc acgcgcacct caaggtgctg gccgatgaca tgctggcgtc gctgccgcag
     6541 ggcagcgtct cggcccaggt gcgccagctg atccgcgagc atccgcgctg gggcaaggag
     6601 cgcattggcg agcagctggg catgagcggg cgcaacctca accgcaagct ggccgccgag
     6661 tccatctcct tcaaaggcct gcgcgaagcc ctgatctacg acatggcctc tgccgcgctg
     6721 cgcgccggcc aaagcgtggc agctgtcagc gagaagctgg ggttttcgga tgaaaacgcg
     6781 ttttcccggg ccttccggcg ctggagcggc ctcacccccg cccagttcgt ccgcggcggt
     6841 gatgccaccc tggcacccga gtgaaccgat aatgctgcat gctttttctg ctctctcccg
     6901 ccaagtccct cgactacgaa acgccgctgg gtgaccagcc gcacacgctc ccgatgttcg
     6961 ttccgcagtc caaggaactg attgcggtgc tgcgcaccca gtcgccccaa cagattgccg
     7021 aactgatgtc gctcagcgac aagctctccg ccctcaacgt ggcgcgctac ctggcgtggt
     7081 cgcccaaggc cacgccgaag aacgcccgcc aggcggtgct ggccttcaat ggcgatgtct
     7141 acgacgggct ggacgccaaa acgctcacgc ccgatgacct ggcctgggcc caggaccatg
     7201 tgtgcatcct cagcggcctg tatggcgtgc tgcgcccgct ggacctgatg caaccctacc
     7261 ggctggagat gggcacgcgc ctgtccaacc ccggcggcaa agacctgtac caattctggg
     7321 gcacgcagat tgcggactac cttaataccc gcctgcgcag tgacgtcagc ccggtggtca
     7381 tcaacctggc cagccaggag tatttcaagg cggtggatac caaggccctg aaagcgcgcg
     7441 tggtcgaatg cgtgttccag gactacagcg ccggccaata caagatcgtc agtttctttg
     7501 ccaagcgtgc gcgtggcctg atggcgcgct atgccgccac gcaccgcctg gtgcagcccg
     7561 aacagctgcg cgccttcgac ctggacggct acgcttgggc tgccgccgag tctacgtccg
     7621 agcgcctggt gtttcgccgc cgcgcagctt aattcttaat caaatcggcc tttagccccc
     7681 gtccataaag cgcaaccagc tatgaaaata agagcaaacg gaatcgacat cgaagtcgaa
     7741 gactcggccc agagccaccc agaaaccgcc gacaagcctg tggtgctgct catcatgggg
     7801 ctgggcatgc agctcatcgc ctggccgccg cagatgctgc agggcctggt ggatgcgggc
     7861 taccgcgtgg tgcggttcga caaccgcgat gtgggcctgt ccagccactt cgaccagctc
     7921 ggcaagccca acctgctgtg ggccagcctg aagtacaaga tcggcctggt gcccgctgcc
     7981 ccctacaccc tgagcgacat ggctgccgat gcattgggcg tgctcgacgc actgggcatt
     8041 gccaaagccc atgtggtggg cgtcagcatg ggcggcatga tcgcgcagcg cgtagccatt
     8101 gccgcaccgc aacgcaccct gagcctgacc agcatcatga gttccagcgg tgcgcgtggc
     8161 ctgccgcagg ccgacccca
//
LOCUS       DAOSJM010000059         8051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8331_length_8051_cov_4.026513, whole genome shotgun sequence.
ACCESSION   DAOSJM010000059 DAOSJM010000000
VERSION     DAOSJM010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8331_length_8051_cov_4.026513"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PKX82_03310"
     CDS             complement(<1..793)
                     /locus_tag="PKX82_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HOF51307.1"
                     /translation="MTTAAEIKHLQEHGLYSNANEHDACGVGFVAHIRGEKSHSIVKN
                     ALKILENLDHRGAVGADKLMGDGAGILIQLPDALYREEMAAQGVTLPPPGEYGVGMIF
                     LPKEHASRLACEQEMERAIKAEGQVLLGWRDVPVNKDMPMSPTVREKEPILRQVFIGL
                     GNDVVVQDALERKLYVIRKTASANIQALKLKHSKEYYVPSMSSRTVVYKGLLLADQVG
                     VYFKDLEDARCVSALGLVHQRFSTNTFPEWPLAHPYRYVAHNGEIN"
     gene            complement(967..1668)
                     /locus_tag="PKX82_03315"
     CDS             complement(967..1668)
                     /locus_tag="PKX82_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOF51308.1"
                     /translation="MARLPRVALPGCVHHVMQRGNNGQRIFETAADYQRMLDLLSAHS
                     RTFGVAVHAYVLMANQFHLLVTPHTETGLPQMMQAVGRQYVRYFNDAHRRTGTLWDGR
                     YRSTVIEAEQFLLADMVYIDQSPVREGLVVQAGEYLWSSFGHYAGQRVDKLLTVHPLV
                     WGLGNTPFAREAAYTEMVQVGINAVQQEALRNAILSGWPLGTPEFVDNLQKGTPRRLT
                     KSSPGRPPIERKTPE"
     gene            complement(1694..2467)
                     /locus_tag="PKX82_03320"
     CDS             complement(1694..2467)
                     /locus_tag="PKX82_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF51309.1"
                     /translation="MSLELYKDAHHCCVMFTNLVEDTEAVQANQFLIVDQDEGVILDP
                     GGNMTYNELSMTMRKYIAPANLDYIVASHADPDIIASLDRWMTATQAKLVISNLWSRF
                     APHFCKLGKTDDRVIGVPDEGAKLRFGKTELWLIPAHFMHSEGNFQFYDPVSKILFSG
                     DLGVSMLSGQQASQFITDLNPLPHGMEAFHRRYMVSNKILKLWVKMVRKLDIAMIVPQ
                     HGSPLKGAAVGQLIDWLDALACGIDLMGPEHYQLPTTTI"
     gene            complement(2514..2981)
                     /locus_tag="PKX82_03325"
     CDS             complement(2514..2981)
                     /locus_tag="PKX82_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51310.1"
                     /translation="MKRWFGLGLVLAASLVLGACATSSPDVIQRGDAQRMAQVEDAVV
                     LSVRTVTVDGSQSGVGATVGGVVGAIGGYGGSGVQREAQVLGVLAGVAGAAVGNTLER
                     MSTREEAVEVLVQLKNGERRAIVQAKAAEVFAAGDPVIIVTTGGKVRVTKAPK"
     gene            complement(3025..3942)
                     /locus_tag="PKX82_03330"
     CDS             complement(3025..3942)
                     /locus_tag="PKX82_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOF51311.1"
                     /translation="MKSRQAAKPMPSAKVSAASPLDAELRPGQSIELLKELHILTRDG
                     KLNQDSRRKLKQVYHLYQFIEKLLQELPANPNGTLTLADHGAGKSYLGFILYDLYFKN
                     LPKSHIYGIETRAELVSKSRELAEKLGFSRMSFINLSVAESAQSEALPGQFDVITALH
                     ACDTATDDAIAFGLQKKARAFVLVPCCQAEVARHLSQSKALSLTRTPLAELWRHPLHT
                     REMGSQITNVLRCLYLEAHGYQVTVTELVGWEHSMKNELILARYTGQKKRAAAARLRS
                     ILEEFGLLELLDTRFTLPRDNDCDNGMAG"
     gene            complement(3939..4529)
                     /locus_tag="PKX82_03335"
     CDS             complement(3939..4529)
                     /locus_tag="PKX82_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HOF51312.1"
                     /translation="MTSQSRSSDEQMLSLTVRWLERAVIGLNLCPFAKSVHVKGQIHY
                     RLSHATQKQELLQVLQEELQALQATDPAVRDTTLLILADCFPDFLDFNDFLQDADDVL
                     YTLGLDGTLQIASFHPRFQFAGTTEDDITNFTNRAPFPVLHLIREASIARAVEAFPDA
                     EMIYEKNMRTLEDLGRAGWKELGLEIEREQDAERQA"
     gene            complement(4551..5747)
                     /locus_tag="PKX82_03340"
     CDS             complement(4551..5747)
                     /locus_tag="PKX82_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOF51313.1"
                     /translation="MSINSALAPYASDPALSRGRRFVQPPAPTRTEFQRDRDRIVHST
                     AFRRLVYKTQVFLNHEGDLFRTRLTHSLEVAQLGRSIARALRINEDLVEAIALAHDLG
                     HTPFGHAGQDALNDSMEAHGGFEHNLQSLRVVDHLEERYPDFDGLNLTFETREGILKH
                     CSRRNALAIEQIEHVYADGNGGVGQRFLNRTEPSLEAQLCNLADEIAYNAHDVDDGFR
                     SGLITLEQLREVPLFAQFSDMALKNHPRLVQPTQSRRWMYESIRLMLSQQVYDAVAAT
                     QIALDAAQVKSVEDVRRAAPLVQFSPDMRRQSTELKSFLFQHLYRHPQVTQTMDMAKR
                     VVRELFDVYVAKPQEMQAGFASRTEAQNGVDSAPHRVVADYIAGMTDRFAAREHERLT
                     GQRLLA"
     gene            complement(5793..6908)
                     /gene="aroB"
                     /locus_tag="PKX82_03345"
     CDS             complement(5793..6908)
                     /gene="aroB"
                     /locus_tag="PKX82_03345"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOF51314.1"
                     /translation="MTANPNTSVSIDLGDRSYSIRIGTQLLDDSQTFSQLPKANSAYV
                     VSNTTVAPLYADRLVRALAGVYKSVHLVALPDGEEFKTWQVLNQIFDALLSNGADRKT
                     VLFALGGGVVGDMTGFAAASFMRGIPFVQVPTTLLSQVDSSVGGKTGINHPLGKNMVG
                     AFYQPLLVVCDLDTLKTLPDRELSAGLAEVIKYGPIADGSFLDWIEQNLPLLLARDGT
                     ALAHAVKRSCEIKAWVVGQDEREAGLRAILNFGHTFGHAIEAGLGYGEWLHGEAVGCG
                     MVMAAHLSQRLGLVDAAYVQRLTSLIHNARLPVVGPILDATDNAGRYLELMRMDKKSE
                     AGEIRFVVLDGIAKAKMLGAPDSMVREVVDSCCNPLL"
     gene            complement(6942..7481)
                     /locus_tag="PKX82_03350"
     CDS             complement(6942..7481)
                     /locus_tag="PKX82_03350"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOF51315.1"
                     /translation="MIFSLVGLPGSGKTTVGRQLARRLQLPFVDSDHAIEARLGCSIR
                     EFFEREGEAKFRDVEEEVIDSLTTGPTGVLSTGGGVVIRPANRQHLRERGQVIYLKST
                     PEELFRRLRHDVNRPLLQVADPLSRLRDLYATRDPQYREIAHFVLETGRPSVATLVNM
                     ILMQLELAGLLPAQQTPSV"
     gene            complement(7486..>8051)
                     /locus_tag="PKX82_03355"
     CDS             complement(7486..>8051)
                     /locus_tag="PKX82_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOF51316.1"
                     /translation="YANAGTFAISLFGESANKFLNLEISALEADGKGKVVSSPRVVTA
                     DQIKALIEQGTELPYQVASSSGATSIAFRKANLKLEVTPQITPEGNIILDLDVTKDTV
                     GQSTPAGFAIDTKHIKTQVLVENGGTVVIGGIFTEDQTTDEAKVPFFGDLPGLGVLFR
                     NKSSSSRKREMLVFITPKMIAEKAIGR"
BASE COUNT         1842 a         2369 c         2106 g         1734 t
ORIGIN      
        1 tgttgatttc accgttgtgc gccacataac ggtacgggtg ggccagtggc cactcaggga
       61 aagtgttggt ggagaagcgc tggtgcacca gacccagggc ggacacacaa cgcgcgtctt
      121 ccagatcctt gaagtacaca cccacctgat ccgccagcaa caggcccttg tagaccacgg
      181 tccggctcga catgctggga acgtaatatt ccttggagtg cttgagtttc agtgcctgga
      241 tattggcgct ggcagtcttg cggatcacgt acagcttgcg ctccagtgcg tcctgcacaa
      301 ccacatcgtt tccgagacca atgaacacct ggcgcaggat aggctccttt tcgcgcacag
      361 tgggagacat gggcatgtcc ttgttgaccg gcacatcgcg ccagcccagc agaacctggc
      421 cctcggcctt gatcgcgcgc tccatctcct gctcgcaggc cagacgggat gcgtgctcct
      481 tgggcaaaaa gatcatgccg acgccatact cacccggggg cggcagggtg acaccctggg
      541 ctgccatttc ttcccggtac aaagcatcgg gcagctggat caggataccg gcgccgtcgc
      601 ccatcagttt gtcggcaccc accgcacccc gatggtcgag gttttcaaga atcttcagcg
      661 cattcttgac gatggagtgg cttttctcac cacggatatg ggcgacaaaa cccacgccgc
      721 acgcgtcgtg ctcattggcg ttggaataga gaccgtgttc ttgaagatgc ttgatttcgg
      781 cagccgtggt catggcgcac tcctgaaaat gacagtggac gacaagaata ccgcatagca
      841 acaaaagtgc caagaaaaat aattggggtc agattcttat taaattctca agaatttact
      901 attgttttat tggggacata tcaaaatcaa tagcagaaaa gtgagtatcg gcaaggaaat
      961 caaccactat tccggagttt tccgctcgat gggaggtctg ccgggactgc ttttggtgag
     1021 tcgccgcggg gtgccttttt gcagattgtc gacaaattct ggtgttccca agggccaccc
     1081 ggaaagaatg gcgttgcgca aagcctcctg ctgcaccgca ttgatcccca cctgcaccat
     1141 ttcggtgtaa gccgcctcac gcgcaaatgg cgtgtttccg agaccccaca ccaacgggtg
     1201 cacggtcaga agtttgtcta cgcgctggcc tgcgtaatgc ccgaagctgg accacagata
     1261 ttcgccagcc tgcaccacca gtccttcccg aacaggactc tgatcgatgt agaccatgtc
     1321 ggccagcaga aactgctccg cttcgatgac ggtagatcga taccgcccat cccacaaggt
     1381 gccggttcgt cgatgcgcat cgttgaaata acgcacatac tggcgaccca ccgcctgcat
     1441 catctgtggg agcccggtct ccgtatgcgg cgtaaccagc aaatgaaact ggttggccat
     1501 aagcacatag gcatgcaccg ccacaccaaa cgtccgagaa tgcgcgctca agagatccag
     1561 cattcgctgg tagtcggccg cagtttcaaa aatacgttgc ccgttgttac ccctctgcat
     1621 cacgtggtga acacaacccg gcaacgcaac acgaggaaga cgagccatgg aaaggactac
     1681 cctggcaaag gtgtcaaatc gtcgtcgttg gcaactggta gtgctctggc cccatcaggt
     1741 caataccgca ggccaacgca tccagccagt cgatcaattg acccaccgca gcgcccttga
     1801 gcggcgatcc atgctgggga acaatcatcg cgatatccag tttgcgcacc atcttcaccc
     1861 atagcttcag gatcttgttg ctgaccatat agcggcgatg gaaggcctcc attccatggg
     1921 gcaatgggtt cagatcggta atgaactgcg aagcctgctg accactcagc atagaaacac
     1981 cgagatctcc ggaaaacaga atcttgctca caggatcgta aaactggaag ttgccctccg
     2041 aatgcatgaa gtgcgcgggg atcagccaca gctcggtttt cccaaagcgt aacttggcgc
     2101 cttcatcggg gacgcctatg acacggtcat ccgtctttcc cagtttgcag aaatggggtg
     2161 caaagcgtga ccacagattg gaaatgacca gtttggcctg cgttgcggtc atccaccgat
     2221 ccagagaagc aatgatgtct ggatcggcat gcgatgcaac gatgtagtca agattggccg
     2281 gcgcaatgta tttgcgcatg gtcatgctca actcgttgta cgtcatgttg ccaccgggat
     2341 ccagaatgac accttcgtcc tgatccacga tcaggaactg attggcttgt accgcctcgg
     2401 tatcttcgac gaggttagtg aacatgacgc agcaatggtg cgcgtctttg tagagttcaa
     2461 gggacataat cagaatccag taagttggtc tgattattac ctgtccaagt cgcttacttg
     2521 ggtgctttgg tcacacgaac cttgccaccc gttgtgacaa taatcactgg atcacctgca
     2581 gcaaacacct ctgcagcctt ggcttgcaca atcgcacgcc gttcgccatt tttgagctga
     2641 accagcactt ccaccgcctc ttcgcgggtg ctcatacgct ccagggtatt acctactgcg
     2701 gcacctgcca cgccagccaa aacgcccagc acttgcgctt cgcgctgaac gccacttcca
     2761 ccatagccgc caatcgcacc gaccacgcca cccacagtag cgcctacacc ggactggctg
     2821 ccatcaaccg tgacagttcg caccgacaaa accacagcat cttccacctg ggccatacgc
     2881 tgtgcatcgc cgcgctggat gacatccgga ctgctggtcg cgcaggctcc cagtaccaac
     2941 gaagctgcaa ggactaaccc caacccaaac catcgtttca taaccgactc ccaaaatatt
     3001 gaaaagacat ccctccagaa cgcatcagcc tgccattccg ttgtcacaat cattgtcgcg
     3061 cggcaatgta aaccttgtgt caagcagttc cagaagtccg aactcttcca gaatcgatcg
     3121 caaacgcgct gctgcagccc gctttttttg tccggtgtaa cgcgccagga tcagctcatt
     3181 cttcatgctg tgttcccaac ccacgagttc ggtcacagtg acttggtacc catgtgcctc
     3241 caggtacaga caacgcaata cattggtaat ctggctaccc atctcacggg tgtgcaaagg
     3301 atgacgccag agttcggcca atggggtacg cgtcagggaa agtgccttac tctggcttaa
     3361 atgccgcgcc acttcagcct ggcagcaggg aaccagcaca aaggcccgcg ccttcttctg
     3421 caatccaaag gcgatggcgt cgtccgtggc ggtatcgcat gcatgcaacg cagtgatgac
     3481 atcgaattgg ccgggcaatg cttccgattg ggcggactcg gccacgctca gattgataaa
     3541 cgacatgcgc gaaaagccca gcttctccgc cagttctctg gatttggaaa ccagttccgc
     3601 ccgcgtctca atgccgtaga tgtgactttt cggcaagttc ttgaaataca ggtcatacag
     3661 gataaatccc aagtacgact tgccggcacc atggtccgcc agggtgagcg tcccattggg
     3721 attggccgga agttcctgta gcagcttctc gataaactga tacaggtgat acacctgctt
     3781 gagtttgcgg cgggaatcct ggttcagctt gccatcgcgc gtaagaatat gcagttcctt
     3841 gagcagttca atggactgcc ccggacgcaa ttccgcatcc agtgggctgg ccgcagatac
     3901 cttggcagat ggcattggct tggctgcttg gcgcgatttc atgcttgcct ctctgcgtct
     3961 tgttcacgtt cgatttccag acccaactct ttccagccgg cgcggcccaa gtcttcaagg
     4021 gtccgcatgt tcttctcgta aatcatttct gcgtccggga aggcctccac agcgcgtgca
     4081 atgctggctt cccgaatcag gtgcaacacc ggaaagggtg cccggttggt gaaattcgta
     4141 atgtcgtcct cggtagttcc ggcaaactgg aacctgggat ggaagctggc gatttgcagg
     4201 gtgccatcca accccagcgt gtacagaaca tcatctgcat cctgcagaaa gtcattgaag
     4261 tccaggaaat caggaaaaca gtctgccagt atcaacagtg tggtgtcgcg cactgcggga
     4321 tctgttgctt gcaacgcctg aagctcttcc tgcagcactt gcagcaactc ttgcttttgc
     4381 gtggcatggc tcaatcggta gtggatctgg cctttgacgt gaacgctttt cgcaaacggg
     4441 cagagattga gcccgatgac cgcgcgctcc agccaacgca ccgtcaaaga aagcatctgt
     4501 tcatcactgg agcgtgattg ggaagtcatc gtggtctttc aaaacagtgg tcaggcaagc
     4561 agacgctgcc ccgtcaatcg ttcatgctcc cgcgcagcga atcggtcggt cataccggca
     4621 atatagtccg ccacaacccg atgcggtgct gaatccacgc cgttttgtgc ttccgtgcgt
     4681 gaagcgaagc ctgcctgcat ttcctgcggt ttggcgacgt agacatcgaa caactcgcgc
     4741 actacacgct tggccatgtc catcgtctgc gtgacctgcg ggtggcggta caaatgctgg
     4801 aatagaaatg acttgagctc ggtcgactgc ctgcgcatat ctggtgaaaa ctgcaccaac
     4861 ggggccgctc ggcgcacatc ctcgacgctt tttacctgtg ctgcatcaag ggctatctgg
     4921 gtcgcagcca ctgcgtcgta aacttgttgg ctgagcatca ggcggatgga ctcatacatc
     4981 caccgccgtg actgcgtcgg ttgaaccagc cgtggatgat ttttcagtgc catgtcgctg
     5041 aattgtgcga acaggggaac ctcccgcaac tgctccagcg tgatcagacc cgatctgaac
     5101 ccatcgtcga catcgtgtgc gttgtaggca atttcgtccg ccagattaca cagctgtgct
     5161 tccaggctcg gctccgtgcg gttgagaaaa cgctggccaa cgccaccatt gccatcggcg
     5221 tacacatgtt cgatttgctc aatagccagt gcattcctgc gtgaacaatg cttgaggatg
     5281 ccttcccgtg tttcaaaagt caggttcagc ccgtcaaaat ccgggtaccg ctcttccaga
     5341 tggtctacca cccgcaagct ctgcaggttg tgttcaaacc caccatgggc ctccatactg
     5401 tcattcagcg catcctgacc ggcatgacca aacggtgtgt gccccagatc gtgcgccagg
     5461 gcaatggcct cgacaagatc ctcattgatt cgcaacgccc gtgcgatgga ccggccgagc
     5521 tgggcgacct ccagcgaatg tgtcaggcgg gtacgaaaca gatctccctc gtggttgaga
     5581 aaaacctggg ttttgtacac aagacggcgg aacgcggtgg agtgcacgat gcggtcacgg
     5641 tcacgctgga actcggtacg ggtcggcgca ggcggctgaa caaagcgcct gccacgcgac
     5701 agtgccggat cactggcata cggcgcaaga gcactattta tagacaaatc acaccctacc
     5761 cctcgccaat gctgcgcgta tagctacact attcatagca aaggattgca gcacgagtcc
     5821 accacttcac gcaccatgga atcgggtgcg ccaagcatct ttgctttggc aataccgtca
     5881 agaacgacga aacggatctc gcccgcctct gacttcttgt ccattcgcat cagttccagg
     5941 tagcgtccag cgttgtcagt ggcgtcgaga ataggaccca cgactggcag acgcgcgtta
     6001 tgaatcaggc tggtcagtcg ctgtacataa gcggcatcca ccaatcccaa acgttgtgac
     6061 aggtgggctg ccataaccat gccgcatcca accgcttcgc catgcagcca ctcgccatac
     6121 ccgagcccgg cttcgatggc atgaccgaac gtgtgcccaa aattcagaat cgcacgcaac
     6181 ccggcctcgc gctcatcctg tccgaccacc caggccttga tctcgcagct tcgcttgacc
     6241 gcgtgggcca gcgcagtacc gtcacgcgca agcaacagtg gaaggttctg ctcaatccag
     6301 tccagaaatg atccgtcagc aatggggccg tacttgatga cctctgccag acctgcactc
     6361 agctcacgat ccggaagcgt tttgagcgta tccagatcgc acaccaccaa gagaggctgg
     6421 taaaacgcac cgaccatgtt cttgcccaaa ggatggttga tgccggtctt gccacccacc
     6481 gacgagtcga cctgcgacag caatgtggtc ggcacctgca caaagggaat cccgcgcata
     6541 aaacttgcgg cggcaaatcc cgtcatgtcc cccaccacgc cgccgcccag cgcaaacaac
     6601 actgttttcc ggtcagctcc gttcgacagc aaagcatcaa agatctgatt cagaacctgc
     6661 catgtcttga actcttcgcc atcgggcaaa gccaccaaat gcacgctctt gtacacgcca
     6721 gccaacgcac ggaccagtcg gtcagcgtac aacggtgcta ctgtcgtgtt actgacaaca
     6781 taagcggaat tggccttggg cagctgcgag aaagtctgac tatcgtcaag cagttgagtg
     6841 ccgatacgaa tggagtagct tctatcgccc agatcgatcg aaacgctggt attggggttt
     6901 gcagtcatac cctagagttt aggggacctt gcactaccaa ttcacaccga gggtgtttgc
     6961 tgcgccggca gcagacccgc aagttcaagc tgcatcagaa tcatattgac aagcgtcgca
     7021 acggacggcc gcccggtttc gaggacgaaa tgcgcgattt cacggtactg aggatcgcgt
     7081 gtggcgtaga gatcccgcaa acgggacagc gggtcagcca cctgcagcaa tggacgattc
     7141 acatcgtggc gcaggcgccg aaacaactcc tctggagtgg atttcaggta aatgacctgt
     7201 ccgcgttcac gcagatgctg tcgattggcc ggacgaatga ccacgccacc gccagtggaa
     7261 agcacaccgg tgggccccgt ggtcaacgag tctatgactt cctcttcgac atcccggaat
     7321 ttggcttcgc cttcgcgttc aaagaactcg cggatagagc agcccagacg cgcttcaatg
     7381 gcatggtctg aatccacgaa aggaagctga agacgccggg cgagttgacg cccgactgtt
     7441 gtctttccgg agccaggcag gccaaccaga ctgaatatca agggatcagc ggccgatggc
     7501 tttctcggca atcatcttcg gggtaatgaa gaccagcatt tcacgctttc ttgaggagct
     7561 ggacttattc cggaagagca cgcccaagcc tggcagatca ccaaagaacg gaaccttggc
     7621 ttcgtctgtt gtttggtctt ccgtaaaaat cccgccgatc acaacagtac cgccattctc
     7681 gaccagcact tgagtcttga tgtgtttggt gtcaattgca aagcccgcgg gagtggactg
     7741 cccaaccgtg tccttggtga catccaaatc cagaatgatg ttgccttctg gtgtgatctg
     7801 cggcgttact tccagcttca agttggcctt gcggaaagca atagacgtag ctccactgga
     7861 cgaggctact tgatatggca attctgtacc ctgctcaatc agcgccttga tctggtctgc
     7921 cgtaaccaca cgggggcttg aaaccacctt gccttttcca tcggcctcaa gcgcagaaat
     7981 ttccaaattc aaaaacttgt ttgcagactc cccgaacaag gaaatcgcga aggttcctgc
     8041 atttgcgtag c
//
LOCUS       DAOSJM010000060         8040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8340_length_8040_cov_3.325110, whole genome shotgun sequence.
ACCESSION   DAOSJM010000060 DAOSJM010000000
VERSION     DAOSJM010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8340_length_8040_cov_3.325110"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PKX82_03360"
     CDS             complement(<1..181)
                     /locus_tag="PKX82_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOF51317.1"
                     /translation="MLNSLPAPLVAFIATLLMVLNIFFWVPILLLFASLKFVLPFKAV
                     RLRIDPILLFIAESWI"
     gene            complement(187..2247)
                     /locus_tag="PKX82_03365"
     CDS             complement(187..2247)
                     /locus_tag="PKX82_03365"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF51318.1"
                     /translation="MASAPQGDLRSLVDLLPIGAYRLGMDGTILRVNPALLRLNGFAS
                     EAQMRAEKGEFIPNPCLDPSRRALFYQTLLQKGRVHNFESEAIRYMTGEHMWIREHAQ
                     LVRDASGTPLYFEGTVEDITRERTAVAALRQSETMLQNVLQTIPDRVWMKDLQGKYIT
                     CNQAFAESLQVTPDMIVGTSDAQWVDEATAQAVQASDALAYQAGRTVLLEEQMRGPDG
                     LTPTLYEIVKTPYLDADGQCVGLVCIARNIQQRKATETQLRDTSEQLELALMSADLGR
                     WDHDLTQEKGYRMDVYSCRMLGRDTEESTKRRAWGHLIHPDDLPQALHAMQAHLHGET
                     SAYEAEYRARHADGRWVWLSSRGKVVQTGQDGSAQRMVGTLMDVSTRKEAEHQLLTTQ
                     AELQATLSAMPDLLIEFNGAGTYRAMHVHGNTELITAVENQIGRSLWDVLPRDAAEVC
                     QAALDETKATGRSDGKQYSLQLSSGKHWFELSLVRKPTIPGEEDRYIAIARNVTERKL
                     AEHAIEHLAFHDSLTGLPNRRLLNDRLHSALLSSYRAQKHAALLFLDLDQFKLLNDNY
                     GHDVGDLLLQEVARRLLQCSRAIDTVARLGGDEFVVLVHDLSVAEDNARLHAATVAHK
                     IIDSLNEPYGIKGQSHVVTPSIGITMFLGKSVSADTLLKQADIAMYRAKDRGRNTVCF
                     YTSQ"
     gene            complement(2259..3035)
                     /locus_tag="PKX82_03370"
     CDS             complement(2259..3035)
                     /locus_tag="PKX82_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF51319.1"
                     /translation="MNTPSLKLQVENIHKRFGSNEVLKGVSLSAHAGDVISIIGSSGS
                     GKSTFLRCINLLEKPNEGRIIVAGEEIRLAPAKSGELNAVDPKQLARMRTKLAMVFQH
                     FNLWAHMTVLQNIIEAPVHVMGVPKDHAVDIARKYLAKVGLEGKEDSYPAHLSGGQQQ
                     RVAIARALVMEPEVMLFDEPTSALDPELVTEVLKVMKNLAQEGRTMVVVTHEMGFARE
                     VANHLIFLHKGLVEEEGNPAQVLANPRSERLAQFLSGSLK"
     gene            complement(3059..4189)
                     /locus_tag="PKX82_03375"
     CDS             complement(3059..4189)
                     /locus_tag="PKX82_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOF51320.1"
                     /translation="MQRIDHPLLSQSLGSQKTLTSFHFGTPGARPKVTIQASLHAEEL
                     PGMLAAHHLRPLLEAADQAGAVLGEVILVPVANPIGLAQRLDHKPMGRFELDTSENFN
                     RHYPDLAKAVFPQVIDTLGSDGQANVAMVRKAMAQYLADWQPTTELESLRRTLLTLSH
                     DADYMLDLHCDCEGVVHFYTEEPCWPQMAPLAHLLGSRAILLAKNSGSGPIDECLSGA
                     WWQLAEAIKGAGISAPLPQGCCSTTIELRGEADVSHALAQGDAAALHAFLQHIGVLAC
                     DIPVQIPAALCQPTPLAGSETLRAPAPGVVVFAAQVGQQMRKGDLVAEVIDPLSGQSH
                     AVTAGVDGVLYARIRDRYITSGGELGKIAGAIPFRTGELLGA"
     gene            complement(4209..4922)
                     /locus_tag="PKX82_03380"
     CDS             complement(4209..4922)
                     /locus_tag="PKX82_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517423.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF51321.1"
                     /translation="MKWHVIFEPQNLELYATGILTTLGLLFSSLGIGAVLALLFALAL
                     TGPWAPLRWIVGAYTYVIRGTPLLIQVYMIYYGLGQLEWIQARWDDVWPWTHFKEPFF
                     CALLAFSLNTAGYTAEMLAGAIRETNAGEIEAAQAFGMSRFQVMLRVVLPSAMRRTLP
                     AYSNEVVMMLQSTSLASAVPSMLDVTAAASRIYSDYYLPFEAYIAAAAIYLVASFCLI
                     GVFRLAEKHFLAYLAPRKH"
     gene            complement(4919..5611)
                     /locus_tag="PKX82_03385"
     CDS             complement(4919..5611)
                     /locus_tag="PKX82_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51322.1"
                     /translation="MQSSYYTAILQGSVLTVGVSLCALLVSIVLGLLGAAAKLSGRPV
                     LVSIASSYTTVIRGVPDLVVMLLVFYGGTIGINHLLELSGSKATIDINPFLAGVLTLG
                     FIYGAYMTETFRGAILSIPKGQMEAAWAFGMGRTQTFLRITAPQMVRYALPGFTNNWL
                     VLIKSTALVSLIGLKEMTYLAKQASAATRSPFAFFLFTAGLFLLYTTVSLYALKKLNA
                     RYSLGTKRGQLS"
     gene            complement(5681..6526)
                     /locus_tag="PKX82_03390"
     CDS             complement(5681..6526)
                     /locus_tag="PKX82_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOF51323.1"
                     /translation="MKKILLAVAVASLLAACGKKEEAAPAPAAAPASAPAAAAPAPEP
                     AVLKVAIDPTYEPFTFKTADGKPTGFDVDIASALCEQVKRKCEFVEQVWDSMIPGLTA
                     KKYDVIISSMSITDERMKEVDFTDKYYNTPSRIVLKKTVKFTDVASIKGKKIGVLKGS
                     TQEKYAMGELKTAGVVVNSYEAQDQVYLDIKSGRLDGTVADYLEVSGGFLSKPEGANY
                     QLVGPDLREPKYYGYGVGVAMRKGEDKLKGELNAAIKTIRSNGTYKTLNDKYFAKYNI
                     DVYGE"
     gene            6695..7771
                     /locus_tag="PKX82_03395"
     CDS             6695..7771
                     /locus_tag="PKX82_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOF51324.1"
                     /translation="MTTLRVALIGYGNAGRIFHAPLIGGVPGLELAVVSSSKPQAVLA
                     DWPQVRVVATPQEAFASPDVDLVVIATGNESHFPLAEQALHAGKHVVVDKPCTVTLAQ
                     TEALRALARSAGKVLTVFQNRRWDADFLSLRQVLASGELGRIVHFESHFDRYRPVVPD
                     RWREQDLPGSGLWFDLGAHLVDQALQLFGAPQDILLDTAVQRDAAQVNDYFHAQLRYG
                     GAQSGLRVLLHAGALVPAQGPRFVVHGTRGSFVKYGLDVQEDALKAGGRPHWDATGEW
                     GRDPQVGTLTIYRQAWTQTSAAPDIAGNYLAYYAGLRDFLRGQADAPPVTPEQVYRAM
                     QLLTLGEHSAQQGRFLPVDPIVPL"
     gene            7825..>8040
                     /locus_tag="PKX82_03400"
     CDS             7825..>8040
                     /locus_tag="PKX82_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51325.1"
                     /translation="MNAPVDVSFFARAAKPLTSYRKYWAARFGTAPFLPMSRAEMDKL
                     GWDSCDIVLVTGDAYVDHPSFGMAVIGR"
BASE COUNT         1461 a         2339 c         2603 g         1637 t
ORIGIN      
        1 aaatccagga ctcggcgatg aacagcagga tggggtcaat ccgcagccgt acggccttga
       61 acggcagcac aaacttcagg gatgcgaaca gcagcagaat ggggacccag aaaaaaatat
      121 tgagcaccat cagcagggtg gcaatgaagg caaccagggg ggcgggcagt gaattgagca
      181 tggtctctat tgtgaagtgt agaagcacac ggtattgcgt ccccggtcct tggcgcggta
      241 catggcaata tcggcctgct tgagcagtgt gtctgcagaa acgcttttgc ccaggaacat
      301 cgtgatgcct atgctgggtg tcacgacatg gctctggcct ttgatcccat agggctcgtt
      361 caggctgtca atgattttgt gggccacggt ggcggcgtgc aggcgggcgt tgtcttcagc
      421 cacgctgagg tcatgcacca gcaccacaaa ctcatcgcct cccagacgcg cgacggtatc
      481 gatggcacgg ctgcactgca gcaaccgccg cgcgacttcc tgcagcaaca ggtcgcccac
      541 atcgtggccg tagttgtcat tgagcagctt gaactggtcg aggtcgagaa acagcagtgc
      601 cgcgtgtttt tgcgcccggt agctcgacag aagcgcactg tgcagccggt cgttgagcag
      661 gcggcggttg ggcaatccgg tgaggctgtc atgaaaagcg aggtgttcga tggcatgttc
      721 ggcgagcttg cgctccgtga cgttgcgtgc aatggcaatg taacggtcct cttcaccggg
      781 aatcgtcggt ttgcgcacca acgagagctc gaaccagtgt ttcccgctgg agagttgcag
      841 gctgtactgc ttgccgtcgg agcggcccgt ggctttggtc tcatcgagtg ccgcctggca
      901 tacctcggcg gcgtctctgg gaagcacatc ccatagagat cgtccgatct ggttctcgac
      961 agcggtaatc agctcggtgt tgccgtggac atgcatggcg cggtaagtac cggcgccatt
     1021 gaattcaatc agcaagtccg gcatggccga cagggtggcc tgcagttcag cctgtgtggt
     1081 gagtaactgg tgttctgctt ccttgcgcgt ggacacgtcc atcagggtgc cgaccatgcg
     1141 ttgggcggat ccgtcctgcc ccgtctgtac caccttgccg cggctgctca gccagaccca
     1201 gcgcccgtcg gcatggcgtg cgcggtattc agcctcatag gcggatgttt ctccatgcag
     1261 atgggcttgc atggcatgca gtgcctgtgg cagatcgtcc gggtggatca ggtggcccca
     1321 ggcacggcgt ttggtacttt cctcggtatc gcggcccagc attctgcagc tgtacacatc
     1381 catgcggtag cctttttcct gcgtgaggtc gtggtcccac cgtcccaggt ccgcgctcat
     1441 gagggccagc tccagctgct cggaggtatc gcgcagttgg gtctcggttg ctttgcgctg
     1501 ctggatgttg cgcgcgatgc ataccaggcc aacacactgg ccatcagcat ccaggtaagg
     1561 tgtcttgacg atttcgtaca gggtgggggt gagcccgtcg ggcccgcgca tctgttcttc
     1621 cagcaacacc gtgcgtccgg cctggtaggc cagggcatcg ctggcctgaa ccgcctgggc
     1681 cgtggcttcg tccacccact gggcatcgct ggtgccgaca atcatgtccg gagtgacctg
     1741 caggctttcg gcaaacgcct ggttgcaggt gatgtacttg ccctgcaaat ccttcatcca
     1801 gacacggtcc ggaatggtct gcagcacgtt ttgcaacatg gtttcgctct gacgcaaggc
     1861 ggcgactgct gtgcgctcgc gggtgatgtc ctccaccgtg ccttcgaagt acagcggagt
     1921 tcctgacgca tcgcgtacca gctgggcatg ctcgcgtatc cacatgtgct ccccggtcat
     1981 gtaacggatc gcctcggact caaagttgtg gacccgcccc ttttgcagca gtgtctggta
     2041 gaacagggcg cgtcgggagg gatcgagaca ggggttggga atgaactcac ccttttcggc
     2101 gcgcatctgg gcctcgctcg caaagccatt gaggcgcagc agggctggat tcacgcgcag
     2161 gatggttccg tccatgccca gccgataggc tccgatgggg agcagatcga cgagcgatcg
     2221 caggtcgcct tgtggtgcgg atgccatgtc cgccgggcct atttcaggct gcccgaaaga
     2281 aactgcgcca ggcgttcgct gcggggattt gccagcacct gggccgggtt gccttcttct
     2341 tccaccaggc ccttgtgcag gaagatcaga tggttggcga cttcgcgggc aaagcccatc
     2401 tcatgggtca ccaccaccat ggtgcgacct tcctgggcca gatttttcat gaccttgagc
     2461 acttcggtga ccagttcggg gtcgagtgcg ctggtaggtt catcgaacag catgacctcg
     2521 ggctccatga ccagggcacg ggcaatggcc acacgctgct gctggccgcc actgaggtgt
     2581 gcgggatagc tgtcttcctt gccttcaagt cccaccttgg cgaggtactt gcgcgcgata
     2641 tccacggcgt ggtctttggg aacgcccatg acatgcactg gcgcttcaat gatgttctgc
     2701 aacaccgtca tatgcgccca caggttgaag tgctgaaaca ccatggccag tttggtgcgc
     2761 atgcgggcca gctgcttggg gtctaccgca ttgagttccc cgcttttggc tggtgccagc
     2821 ctgatctctt ctccggccac gatgatgcgg ccttcgttgg gtttttccag caggttgatg
     2881 cagcgcaaaa aagtgctctt gcccgagcca gagctgccaa tgatgctgat gacatcgcct
     2941 gcatgggccg acagggagac tcccttgagc acctcgttgg acccaaagcg cttgtggatg
     3001 ttctcgacct gaagtttgag ggacggggta ttcatgtgtt gattctcaag gcgtgggctc
     3061 aggcgcccag caactcgccg gttcgaaagg gaatggcgcc ggcaatcttg ccgagttcac
     3121 caccgctggt gatgtagcgg tcgcgaatgc gggcgtacaa cacgccgtcc acacccgccg
     3181 tgacggcgtg gctctggccc gaaaggggat caatgacttc cgcgaccagg tcgcccttgc
     3241 gcatctgctg gccgacctgg gctgcgaaca ccacgacacc cggggcgggc gcacgcagtg
     3301 tttcggaccc ggccaggggg gtgggctggc acagggctgc gggaatctgg acagggatat
     3361 cgcaggccag cacaccgatg tgctgcagga atgcgtgtag cgcagcggca tcgccctggg
     3421 ccagcgcatg cgacacatcg gcttctccgc gcagctcgat ggtggtgctg cagcagccct
     3481 ggggcaaggg ggcgctgatg ccggcaccct ttatggcctc ggccagctgc caccaggcgc
     3541 ccgacagaca ctcgtcaatc gggccgctcc ccgagttttt ggcgagcagg atagcgcggc
     3601 tgcccagcag gtgggccagg ggcgccatct ggggccagca gggttcttcg gtgtagaaat
     3661 gcaccacccc ttcgcagtcg caatgcaggt ccagcatgta gtcggcgtcg tgcgacagcg
     3721 tcagcagggt gcggcgcaga ctctccagtt cggtggtggg ctgccagtcg gccaggtact
     3781 gggccatggc cttgcgcacc atagcgacat tggcttgccc atcgctgccc agggtgtcta
     3841 tgacctgggg gaagacggct ttggccaggt cgggatagtg gcggttgaag ttttccgacg
     3901 tgtcgagttc aaaccgtccc atgggcttgt ggtccaaccg ctgggccagg ccgatgggat
     3961 tggccacagg gaccagaatg acttcgccca gcacggcgcc cgcctggtcc gccgcttcca
     4021 gcaagggtcg caggtggtgc gcagccagca tgcccggcag ctcttcggcg tgcaggctgg
     4081 cctggatggt gaccttgggc cgcgcgcccg gtgtaccaaa gtgaaaactg gtcagggtct
     4141 tctgggaacc gaggctttga gacaggaggg gatggtcaat gcgttgcatg gatggctcgg
     4201 gttacgcgct agtgctttct gggcgccaga taggccagaa aatgcttttc cgccagacgg
     4261 aacacaccaa tcaggcagaa agatgccacc aggtaaatgg ctgcagcggc aatatacgct
     4321 tcaaacggca ggtagtaatc cgagtagatg cggctggccg cggccgtgac gtccagcatg
     4381 ctgggcactg cactggccag gctggtgctt tgcagcatca tgaccacttc gttgctgtag
     4441 gccggcaggg tgcggcgcat ggcgctgggc aacaccactc gcagcatgac ctggaagcgg
     4501 ctcatgccaa aagcctgggc tgcttcaatc tcgcctgcgt tggtttcacg gatggcgcca
     4561 gccagcattt cggcggtata gcccgcggtg ttcaggctga acgccagcag agcgcagaag
     4621 aaaggttcct tgaagtgggt ccagggccag acgtcgtccc agcgggcctg aatccactcc
     4681 agttgcccca ggccgtaata gatcatgtag acctgaatca gcagcggggt tccgcgaatc
     4741 acataggtat aggcacccac gatccagcgc aggggtgccc agggcccggt cagtgccagc
     4801 gcaaacagca gggccagcac ggcgccaatg cccagcgatg aaaacagcag gcccagggtg
     4861 gtgaggatgc cggtggcata cagctccaga ttctgcggtt cgaaaatgac atgccacttc
     4921 atgacagttg cccccgcttg gtgcccaggc tgtagcgtgc gttgagcttt ttcagtgcat
     4981 acagcgagac ggtggtgtac agcaggaaca ggccggcggt gaacaggaag aaggcaaacg
     5041 gtgagcgggt cgctgcgctg gcctgcttgg ccaggtaggt catttctttc aggccaatca
     5101 ggctgaccag cgcggtggac ttgatcagta ccagccagtt attggtaaag ccgggcaggg
     5161 cgtagcgcac catctgggga gcggtgatgc gcaggaaagt ttgcgtgcgt cccatgccga
     5221 aggcccaggc cgcttccatt tgtcccttgg gaatcgacag aatggcgccc cgaaaggttt
     5281 cggtcatgta agcgccgtaa atgaagccca gggtcagaac accggccaga aatgggttga
     5341 tgtcgatggt ggctttgctg cccgagagct cgagcaggtg gttgatgcca atggtgccac
     5401 cgtaaaaaac gaggagcatg acgaccaggt ccgggacgcc ccggatcacg gtggtgtagc
     5461 tcgatgcaat ggataccagt accgggcggc ccgacagctt ggccgctgca cccagcagcc
     5521 ccagcacgat agataccagc aaggcacaca gggaaacgcc caccgtcagt accgagccct
     5581 gcagaatggc cgtgtagtag gaagattgca tggatgggca ggctgtaaaa acaaaagcga
     5641 cggtacgccg aaccagcgcg ccgtcgcttg tctgttgcga ttactcgccg tagacgtcga
     5701 tgttgtactt ggcgaagtac ttgtcgttga gcgtcttgta ggtgccgttg ctacggatgg
     5761 ttttgatggc agcgttcaac tcgcccttga gcttgtcttc acccttgcgc atggcaacgc
     5821 ctacgccgta accatagtac ttgggttcac gcaggtccgg gcccaccagc tggtagttgg
     5881 cgccttccgg cttggacagg aaaccgccgg acacttccag gtagtcggcc acagtaccat
     5941 ccaggcggcc ggacttgatg tccaggtaga cctggtcctg ggcttcgtag gagttcacca
     6001 ccacaccggc agtcttgagt tcacccatgg cgtacttctc ttgggtagag cccttcagaa
     6061 caccaatctt cttgcccttg atggaagcaa cgtcggtgaa cttgacggtt ttcttcagca
     6121 cgatgcggct gggggtgttg tagtacttgt cggtgaaatc cacttccttc atgcgttcgt
     6181 cggtgatgga catggagctg atgatcacgt cgtacttctt ggcggtcagg ccggggatca
     6241 tgctgtccca cacctgctcg acaaattcgc acttgcgctt gacctgctcg cacagggcgc
     6301 tggcgatgtc cacgtcaaag ccggtgggct tgccgtcggc ggtcttgaaa gtgaagggtt
     6361 cgtaggtagg gtcgatagcg accttcagca ctgcgggttc aggtgctggt gcagcagcgg
     6421 cgggtgcaga agcaggcgct gcggcgggtg ccggggcagc ttcttccttt ttgccacatg
     6481 cggccaacag cgatgcgaca gccactgcca gaagtatttt cttcatgtct gatccttgaa
     6541 aacccggaga tagtgatccg ggagggttaa tgaaaactga aaaatcgatt ttacgggcat
     6601 cggtatgcaa ttttggtgcc gcccgagact ttggggttgg ggtttgtacg gtgacgcagg
     6661 agcccatctc gtctttttgc gcgatgattg gccgatgaca acccttcgcg tcgcactgat
     6721 tggctacggc aatgctggcc gcatttttca tgcccccctg atcggcgggg tgccgggcct
     6781 ggaactggct gtggtcagct ccagcaaacc acaggccgtg ctggcagact ggccgcaggt
     6841 gcgtgtggta gccactccac aggaggcttt tgcgagtcct gacgtggatc tggtggtgat
     6901 tgccaccggc aacgagagcc atttcccgct ggccgagcag gcgttgcatg ccggcaaaca
     6961 tgtggtggtg gacaagccct gcaccgtcac gctggcccaa acggaggcgc tgcgggcgct
     7021 ggcccggtct gcgggcaagg tgcttaccgt gttccagaac cgccgctggg atgcggactt
     7081 tctgtccctg cggcaggtgc tggcgagtgg cgaactgggg cgcatcgtgc acttcgagtc
     7141 gcactttgac cgctaccgcc cggtggtgcc agaccgctgg cgcgaacagg acttgccggg
     7201 ctcgggcctc tggtttgatc tgggggcgca cctggtggac caggccctgc agctttttgg
     7261 tgccccgcag gatatcctgc ttgacaccgc cgtccagcgc gatgccgccc aggtcaatga
     7321 ctactttcac gcccagctgc gctacggcgg agcccagtcc ggcttgcggg tgctgctgca
     7381 tgcgggggcg ctggttcctg cgcaggggcc ccgctttgtg gtgcacggca cacgcggcag
     7441 cttcgtcaaa tacgggctcg acgtacagga ggacgcgctc aaggctggtg gtcggccgca
     7501 ctgggacgct accggggagt ggggccggga cccccaggtt ggcacgctga ccatctaccg
     7561 gcaggcatgg acgcaaacgt ctgcagctcc cgacatcgcg ggcaactacc tggcctatta
     7621 cgccgggctg cgggatttcc tgcgggggca ggccgatgcc cccccggtca ccccggagca
     7681 ggtctaccgc gccatgcaac tgctgaccct gggcgaacac agtgcgcagc aggggcgatt
     7741 tctgcccgtg gaccccatag tgcccttata gggtgtgttg ccaagcgcaa gcccgtacac
     7801 tcggggaccc atttcccgag tgccatgaac gcccctgttg acgtctcctt tttcgcgcgt
     7861 gctgccaagc cgctgaccag ttaccgcaag tactgggcgg cccgctttgg cacggccccc
     7921 ttcctgccca tgagccgcgc cgagatggac aagctgggtt gggacagctg cgacatcgtt
     7981 ctggtgaccg gcgacgccta tgtcgaccac cccagttttg gcatggcggt gattggccgc
//
LOCUS       DAOSJM010000061         8020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8362_length_8020_cov_4.833898, whole genome shotgun sequence.
ACCESSION   DAOSJM010000061 DAOSJM010000000
VERSION     DAOSJM010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8362_length_8020_cov_4.833898"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..1449)
                     /locus_tag="PKX82_03405"
     CDS             complement(28..1449)
                     /locus_tag="PKX82_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOF51326.1"
                     /translation="MSVIPALLPSPSRRQRVTTETRALWHLAWPILIGQLANIGMAVV
                     DVAMAGHASADDLAGISLGVSIWNMVIITIMGLMMSVNPMVAHHVGARELDQVPHVVR
                     QGLWKALAVGLVAMLAANLAGLVFESMVLEPQVRDLARSFVLVTSFALPAFACYRVLY
                     GYSTSLNQTKPLMVISLGALALNVLVNWLLVFGNWGFPRLGGLGCAWSTLLCVWFNLL
                     ALLWWMRRASAYRATWPLGEYEAPHWPKLRSLFRLGLPIGVTYFAETSAFGLIALLVA
                     KFGSHEIAAHQIALNFTSLVFMVPLSLGIALLTRVGQSLGAGDPAAARFRAWVGVGVS
                     VVFAVISATGMALFNTSIASAYTSDADVAALAAQLLFFAAIFQLSDATQVVTSCAIRG
                     YKVARVPMVIHLTAFWVFSLPLGCVLGLAPDWLPWRPAQPMGAAGFWIALIVGLTIAA
                     LGLVVLLRQTARAHLHSTRLLAT"
     gene            complement(1475..2659)
                     /locus_tag="PKX82_03410"
     CDS             complement(1475..2659)
                     /locus_tag="PKX82_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOF51327.1"
                     /translation="MPRFSLHAIAAVIAVSGLLAACSTGPTAQSTAAKYPTLDGALPL
                     VIGHRGASGYRPEHTLASYQKAIELGADFIEPDLVVTKDGELVARHEPNITATTDVST
                     RAEFASRKTTRKVDGVNETGWFATDFTLAELRTLRAKQPNAARDKSFDGQFQIPTFRE
                     ILNLAKTESARTGRTIGVYPETKHPTYHVDAGLPIEPRLLALLNEYGYTQKTSPVIIQ
                     SFEVANLKALRKLTNVRLVQLIDGDDVDSKGNVTLVAPFDKPYDFAVAKDSRNFPDML
                     TPKGLAEIKSYADGIGPWKPYLASAAHVMDTHGKPKDLNGDGKISDADRVALPPTDVV
                     KNAHAAGLFVHAFTFRNEAPGLLSDYKGDPKAEYKRFFHLGVDGLFSDFPDTAIAARA
                     AP"
     gene            complement(2728..4002)
                     /locus_tag="PKX82_03415"
     CDS             complement(2728..4002)
                     /locus_tag="PKX82_03415"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOF51328.1"
                     /translation="MSTSRRTVMLGGAAAVAAVAVAASKPRDQGGPYPAYFEKLNQTL
                     KAHQIDRPVLVIDLDRLDRNIDRVARSASTAPAKTYRIVVKSVPSPALVDYIARRAHT
                     NSLMVFHRPFLQAMATLRPDSDILLGKPMPLAAAQTFYAQHKGAFDPARQLQWLIDTD
                     ARLQQYQTLAHKLGIRMRVNLEIDVGLRRGGFADPAALLPALQRIQADPQHLEFAGFM
                     GYDAHIMGLPGFLADREKPKVRARYAACVDLLKTQFAALATGKLTFNGAGSPTFRLYE
                     GDAVINDLSAGSCLMKPTHYDLPILEDFEASAFIASPVLKHQPARLPALDVVSSLMGT
                     WNPNQEDIYFAYSGNWLAECEAPPGLAPHFAYVSSNQQGYTASKSVSLQVDDFIFLRP
                     TQSEAVLLQFGDLVGVRGDKVEQRWSVLPVAA"
     gene            complement(4004..5431)
                     /locus_tag="PKX82_03420"
     CDS             complement(4004..5431)
                     /locus_tag="PKX82_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HOF51329.1"
                     /translation="MKFPRTMNRRTVLRAGTALAAGAATGGVLQGCSPEVTEPQAPLP
                     FTSGKPLPWTNWAGNQACTPAARAVPASEVEVIDVLRNAKGTVRAAGASHSFSAVVPT
                     DDTLVSTDLLSGLVRHDPTTQQAEIWAGTRMHALGPMLHSVGQALPNMPDMDYLSMGG
                     AIANSAHATGTGFGSMSSYVAGLALATPSGELIECSATRNPEVFQAARTSVGALGIVT
                     RFTLQNDKAFALTEVNRVETTEDVLADIDNRCRQHRHFEFMPLLHSDLCVTIATDLAQ
                     PGDKDEGEDDPQAINTLRSVFNAVRWLPGGDALYNQALTTFMAGDASTVRTGPSFQVF
                     PHVRVVRFREMEYTVPAEVGPACVREILATVKQRNIPVCFPLEYRYIKADDIWLSMFE
                     GRDGCSISVHQFGDVDFKPYFAEIEPIFWKYEGRPHWGKIHTLDARRLAALYPRHWKD
                     FQEVRRALDPQGRLLNAHLKTLFGA"
     gene            complement(5428..5757)
                     /locus_tag="PKX82_03425"
     CDS             complement(5428..5757)
                     /locus_tag="PKX82_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOF51330.1"
                     /translation="MKYANLAALGAIAWAGLQPWAAHAELTPRQNALLANNCLQCHAR
                     PSTGAPVIGKADDWSARLAKGDTALLRNTVEGYKGMPPMGYCSACNEDDFRALIAQLA
                     GKEIGTP"
     gene            5866..6882
                     /locus_tag="PKX82_03430"
     CDS             5866..6882
                     /locus_tag="PKX82_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOF51331.1"
                     /translation="MYLAQREPWSVPLQMVGLIAQELQSQGVAVEDYLQGSPLCPQDL
                     ATPDKPIAYRQMLELIRRGVTLSRRAGLGLDVGRRQTPAGLGVLGYAINCCATARDAL
                     LTGAKYHAVASSINRLALSEDARHAYWIATPPIDLQEALPFAIEEEFSAFCRNSELLT
                     GKKAPLVEVHFTYDAPAYVYRYQEAFDAALRFNAPTNQIVFDVGALDLPILQGNPLSV
                     AHGVQLCENFLANNPSTDDLTLRICAMLLRTPGVFPPAEEVAASLHMSARTLRYQLAQ
                     IGQSYQAIVERLKLQLSRDYLENSPLKVEDIAWRLGYLDARNFRRAFKAWTGVTPSEY
                     RRTG"
     gene            6994..>8020
                     /locus_tag="PKX82_03435"
     CDS             6994..>8020
                     /locus_tag="PKX82_03435"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF51332.1"
                     /translation="MTKNIEGDRWIVRTNYLNRSSSFLIMFAVVGAQMAVRGYGVLAW
                     CLVALLFLVYPHLAFLRARRSATPHAAELNNLLVDSFLFGMCAAGLDFPLWICFTLFI
                     GTAITHVLYLGITRIFRSLAAIALGVALTVGFRGVRPLADTEMSAVVLCIIGLSLYLL
                     SLANIAYFGTRKLLDSRAKLHVSEKVLHEANAALQSQLDQIQTLQSQLSEQAHRDPLT
                     GLYNRRYLDTAMARELARCQREGQPLSVLMLDLDFFKRVNDQFGHAAGDEVLKGLATL
                     LTQHTRSSDVACRFGGEEFLVLLPNMSLAAALDKAEQWRQAFAAQTVLWDGSPIRTTL
                     SIGVATFP"
BASE COUNT         1457 a         2340 c         2675 g         1548 t
ORIGIN      
        1 gatgcgggta tctgggtggg gcatggatca ggtcgccagc aggcgggtgg aatgcaggtg
       61 ggcgcgggcg gtctggcgca acagtaccac cagaccgagt gctgcaatgg tgaggcccac
      121 aatcagcgca atccagaacc ctgcggcccc catgggctgt gcaggccgcc agggcagcca
      181 gtcgggcgcg aggccgagca cgcagcccag tggcagggaa aaaacccaga aggccgtgag
      241 gtggatgacc atgggcacgc gggccacctt gtagccgcgg atggcgcagc tggtgaccac
      301 ctgggtggca tccgacagct ggaagatggc agcgaagaac agcaactgcg ccgccagtgc
      361 ggccacgtcg gcatcgctgg tgtaagcgct ggcaatcgag gtattgaaca gtgccatgcc
      421 ggtggcagag atcaccgcaa acaccaccga aacccccacg cccacccagg cccggaaccg
      481 tgcggccgca ggatcgcctg cgcccaggct ctggcccacg cgggtgagca gcgcaatgcc
      541 cagactcagc ggcaccatga acaccagtga ggtgaaattc agcgcaatct ggtgggcggc
      601 aatctcgtgg ctgccgaatt tcgccaccag cagggcaatc aggccaaagg cactggtttc
      661 ggcaaagtag gtcacgccaa tgggcagacc cagacggaac aggctgcgca gcttgggcca
      721 gtgcggagct tcgtattcgc ccagcggcca ggtggcacgg tacgcgctgg cgcggcgcat
      781 ccaccacagc agtgccagca ggttgaacca cacacacagc aaggtggacc aggcgcagcc
      841 cagaccaccg aggcggggga atccccagtt gccgaacacc agcagccagt tcaccagcac
      901 gttgagtgcc agtgcaccca gcgaaatcac catcaggggt ttggtctggt tcaggctggt
      961 gctgtagcca tagagcacgc ggtagcaggc aaacgctggc agggcaaagc tggtgaccag
     1021 cacaaagctg cgtgccaggt cgcgtacctg tggctccagc accatggatt caaataccag
     1081 gccggccagg ttggctgcca gcatcgcgac caggcccacc gccagtgcct tccacaggcc
     1141 ctggcgtacg acatggggca cctggtccaa ctcgcgcgcg cccacgtggt gggcgaccat
     1201 ggggttgacc gacatcatca gccccatgat ggtgatgatc accatgttcc agatcgacac
     1261 ccccagcgag ataccggcca ggtcatcggc cgaagcgtgc ccggccatgg ccacatcgac
     1321 cactgccatg ccgatgttgg ccagttgtcc gatcaggatc ggccacgcca gatgccacag
     1381 ggcgcgggtc tctgtggtaa cccgctggcg gcgtgatggg gagggaagca aggcaggaat
     1441 gacagacatc ggtctgccat tctaggtggc tgccttacgg tgctgcgcgt gcggcgatgg
     1501 cggtgtcagg aaagtcgctg aacagtccat cgacgccgag gtggaagaag cgtttgtact
     1561 cggccttggg gtcacccttg tagtcggaca gcagccccgg ggcctcgttg cggaaggtga
     1621 aggcgtggac gaacaggccg gctgcgtggg cgttcttgac gacatcggtc ggtggcaatg
     1681 ccacgcggtc ggcatcgctg atcttgccgt cgccattgag gtctttgggc ttgccatggg
     1741 tgtccatcac atgggcggcg ctggccaggt agggcttcca ggggccaatg ccatcggcat
     1801 agctcttgat ctctgccagg cctttggggg tcagcatgtc ggggaagttg cggctgtctt
     1861 tggccacggc aaagtcgtag ggcttgtcga aaggcgcgac cagagtgacg ttgcccttgg
     1921 agtccacatc gtcgccgtcg atcagctgca ccaggcgcac gttggtgagc ttgcgcaggg
     1981 ccttgaggtt ggccacttca aacgactgga tgatgaccgg agaggttttc tgggtgtagc
     2041 cgtactcatt cagcagagcc agcaggcgcg gttcaatggg caggcccgcg tccacgtggt
     2101 aggtggggtg ctttgtttcg gggtacacgc cgatggtgcg accggtccgg gccgattcgg
     2161 tcttggcgag attgagaatt tcgcggaagg tcgggatctg gaactggccg tcaaacgact
     2221 tgtcgcgcgc cgcgttgggc tgtttggcgc gcagggtgcg caactcggcc agcgtgaagt
     2281 cggtggcaaa ccagccggtc tcattcacgc cgtcgacctt gcgggtggtc ttgcggctgg
     2341 caaattcggc gcgggtggag acgtcggtgg tggcagtgat gttgggttcg tggcgcgcca
     2401 cgagttcacc gtctttggtc acgaccagat caggctcgat gaagtcggcc cccagttcaa
     2461 tggccttctg gtaggacgcc agcgtgtgct cggggcggta gccactggca ccgcggtggc
     2521 cgatgacgag cggcagcgcg ccgtccagcg tggggtactt ggcagcggtg ctctgggcgg
     2581 tggggcccgt ggagcaggca gccagaagac cggagactgc aatgacggca gcgatagcgt
     2641 ggagcgaaaa gcgtggcatg gatttcctct tgtgtgtgtg acaacttcag cggggaatca
     2701 tggggtgcaa atgtgacagt ggcgtgacta ggctgcgact ggcagcactg accagcgttg
     2761 ttccaccttg tcgccgcgca cgccaaccag gtccccgaat tgcagcagca cggcctccga
     2821 ctgggtgggg cgcaggaaga tgaaatcgtc cacctggaga gacaccgatt tcgaggcggt
     2881 atagccctgc tggttggagc tgacgtaggc aaagtggggc gccagccctg gaggggcttc
     2941 gcattccgcc agccagttgc cgctgtaggc aaagtagatg tcttcctggt tggggttcca
     3001 ggtgcccatg agcgaactca ctacatcgag cgccggcaat cgggccggct ggtgtttgag
     3061 taccggggat gcaatgaatg cgctggcctc gaagtcttcc agaattggca ggtcatagtg
     3121 cgtgggtttc atcaggcacg accctgcaga caggtcgttg atcacggcgt cgccctcgta
     3181 gagccggaag gtggggctgc cggcgccgtt gaaggtgagt ttgccggtgg ccagtgccgc
     3241 gaattgggtc ttgagcaggt ccacacacgc ggcatagcgg gcccgaactt tgggtttctc
     3301 gcggtctgcc aggaatccgg gcaagcccat gatgtgggcg tcgtagccca tgaaaccggc
     3361 aaactccagg tgctgcgggt cagcctgaat gcgttgcaga gccggtagca gggcggccgg
     3421 gtcggcaaac ccgccacgac gcaagcccac atcgatctcc agattgacgc gcatcctgat
     3481 gccgagcttg tgcgccagcg tctggtactg ctgcagccgc gcatcggtgt cgatcagcca
     3541 ttgcaactgg cgcgcgggat cgaaggcgcc cttgtgctgt gcgtaaaagg tctgcgcggc
     3601 agccagcggc atgggcttgc ccagcaggat gtcggagtcc ggtcgcaagg tggccatggc
     3661 ctgcaggaag gggcggtgga acaccatgag tgagttggtg tgggcccgcc gggcaatgta
     3721 gtccaccaga gccggactgg gcaccgactt gaccacgata cggtaggtct tggcgggcgc
     3781 agtgctggcc gacctggcta cccggtcaat gttgcggtcc agcctgtcca ggtcaatcac
     3841 cagcaccggg cggtcgatct ggtgcgcctt gagagtctgg ttgagttttt caaagtaggc
     3901 cgggtaaggg ccaccctggt cgcggggttt gctggctgct acggccacgg cggcgaccgc
     3961 ggcggcaccc cccaacatga cggtacggcg ggatgtggac atatcaggct ccaaaaaggg
     4021 ttttgagatg ggcgttgagc agccgcccct gggggtcgag tgcgcgtcgc acttcctgga
     4081 agtctttcca gtggcgtggg tacagggctg ccaggcgtct ggcgtccagc gtgtggattt
     4141 ttccccaatg cgggcggcct tcgtatttcc agaaaatcgg ttcgatctcg gcaaagtacg
     4201 gtttgaagtc gacgtcgccg aactggtgca ccgaaatact gcaaccatcg cgtccttcaa
     4261 acatggagag ccagatgtcg tcggccttga tatagcggta ctccagcggg aaacacaccg
     4321 ggatgttgcg ctgtttcacc gttgcgagaa tctcgcgcac gcacgccgga ccgacctcgg
     4381 cgggcacggt gtattccatt tcgcggaaac gcaccacgcg cacatgcggg aagacctgga
     4441 aggacgggcc agtgcgcacc gtgctggcgt cacccgccat gaacgtggtg agagcctggt
     4501 tgtatagcgc gtccccaccg ggcagccaac gcacggcatt gaacacgctg cgcagggtgt
     4561 tgatggcctg tgggtcgtcc tccccctcgt ccttgtcgcc cggttgggcc aggtccgtgg
     4621 caatggtcac gcacagatcg gagtgcaaca gtggcatgaa ctcgaaatgc cggtgctgtc
     4681 tgcaacggtt gtcgatgtct gccagcacgt cctcggtggt ctcgacgcgg ttgacctcgg
     4741 tgagggcaaa ggccttgtcg ttctgcagcg tgaagcgggt gacgatgccc agcgcgccga
     4801 ccgaagtacg tgccgcctgg aatacctcgg ggttgcgggt ggcgctgcat tcgatcagct
     4861 cacccgatgg cgtggccagc gccaggccag ccacatagga cgacatggat ccaaacccgg
     4921 tgcctgtggc gtgcgcggag ttggcgatgg ctccgcccat ggatagatag tccatgtccg
     4981 gcatattcgg cagggcctgg cccacactgt gcaacatcgg ccccagtgcg tgcatgcgtg
     5041 tgccggccca gatttcggcc tgctgggtgg tggggtcgtg ccggaccagt ccgctcagca
     5101 gatcggtgga caccagggtg tcgtcagtgg gtaccacggc gctaaaagaa tggcttgcgc
     5161 cagcggcacg taccgtgcct ttggcgttgc gcagaacatc gatcacttcg acttcggacg
     5221 cgggtactgc gcgggcggct ggggtacacg cctggttgcc agcccagttg gtccagggca
     5281 ggggcttgcc actggtgaag ggcaaaggcg cctggggttc ggtgacttca gggctgcagc
     5341 cctgcaaaac gccgcccgtt gcggcacctg cagccagtgc ggtaccggcg cgcagcacgg
     5401 tgcgtcggtt catggtgcgt gggaatttca tggtgtaccg atctccttgc cggccagctg
     5461 ggcaatcagg gcacgaaagt cgtcttcatt gcaggcactg cagtagccca tggggggcat
     5521 gcccttgtag ccctccaccg tattgcgcag caatgcggtg tcgcctttgg ccaggcgcgc
     5581 gctccagtca tcggccttgc caatcaccgg ggcgccggtc gatgggcgcg cgtggcattg
     5641 caggcagttg ttggccagca gtgcgttttg tctgggtgtg agttcggcat gggcagccca
     5701 tggctgcagt cccgcccagg cgatagcccc caaggcggcc aggttcgcgt acttcaatgg
     5761 agtctcctct ggtttttctt ggccaaagca tagggaagtc ccgggagaca cacgaggtca
     5821 tatccggtaa attgagggtc atttccggca acatacacaa gctccatgta tctggcacaa
     5881 cgtgaaccgt ggtctgtccc cctgcaaatg gtcgggctga tcgcgcagga attgcagtcg
     5941 cagggcgtgg cggtggagga ctatctgcag ggcagccccc tgtgccccca ggacctggct
     6001 acacccgaca aacccattgc ctaccggcag atgctggaac tcatccgccg gggtgtgact
     6061 ctctcccggc gtgcgggact ggggctcgac gtgggacgcc ggcaaacccc agccggcttg
     6121 ggggtgctgg ggtacgccat caattgctgc gccactgccc gcgatgcgtt gctgaccggt
     6181 gccaaatacc atgcggtggc ttcgtccatc aaccggctcg cgctgtcgga ggatgcgcgc
     6241 cacgcctact ggattgccac ccctcccatc gacttgcagg aggcacttcc ttttgcgatt
     6301 gaggaggagt tttctgcctt ctgccggaac tcggaattgc tgaccggaaa aaaggcaccg
     6361 ctggtggagg tgcatttcac ctacgacgcg ccggcctatg tgtatcgcta ccaggaggca
     6421 tttgatgcag cgctgcgatt caacgcgccc accaaccaga tcgtgttcga tgtgggcgcg
     6481 ctggatctgc ccattcttca gggcaatcca ttgagtgttg cgcatggtgt ccagttgtgt
     6541 gaaaacttcc tggcgaacaa ccccagcacc gatgacctga ccctgcgcat ctgcgcgatg
     6601 ctgttacgca ctccgggcgt gtttccaccc gcagaagagg tggctgccag cttgcacatg
     6661 tcggcacgca ccctgcgcta ccagcttgcg caaatcggac agagctatca ggccatcgtg
     6721 gagcggctga aactgcagtt gtcccgggac tacctggaga actcccctct caaagtggaa
     6781 gacattgcct ggcgtctggg ctatctggat gccagaaatt tccgacgtgc cttcaaggcc
     6841 tggaccgggg tgaccccgag tgaataccgg cgcacgggct gagtcccctg tatgacaatg
     6901 gggcatggaa gagtctggca gcatttcacc gcgggcgaat aaaattcaga gttgaagtct
     6961 gaccatgctg tgccagacaa ccggatcctt tctgtgacca agaatatcga aggcgaccgc
     7021 tggatcgtcc gaaccaatta cctgaaccgg tccagttcgt tcctgatcat gttcgcggta
     7081 gtcggtgcgc aaatggctgt gcggggctat ggcgtgctgg cctggtgcct ggtggccctg
     7141 ctgtttctgg tgtatcccca tctggcgttt ctgcgggcgc gacgttctgc cacaccccac
     7201 gcggcagagt tgaacaatct gctggtggac tctttcctgt ttggtatgtg tgccgcggga
     7261 ctcgatttcc cgttgtggat ttgtttcacg ctgttcatcg gtaccgccat cacccatgtc
     7321 ttgtacctgg gtatcacgag aatttttcgg tccctcgctg ccatcgcgct gggtgtggcg
     7381 ctgacggtgg gctttcgggg tgtccgcccg ttggcagaca cggaaatgtc tgctgtggtg
     7441 ttatgcatca tcgggctctc gctgtacctg ctgagcctgg cgaatattgc gtacttcggg
     7501 acgcgcaagt tactggactc ccgcgcgaaa ttgcatgtga gcgagaaagt gctccacgag
     7561 gccaacgccg ccttgcagtc gcagctggac cagatccaga ccttgcaatc ccagctcagt
     7621 gagcaggccc atcgggatcc cctgacgggc ctgtacaacc gccgctacct tgataccgcc
     7681 atggcgcgtg aactggcgcg ctgccagcgc gaggggcagc ctttgagcgt gctcatgctg
     7741 gacctggatt ttttcaaacg ggtgaatgac cagttcggtc atgccgcagg cgatgaggtt
     7801 ctgaaaggtc tagccacctt gctgacacag cacacgcggt ccagcgatgt ggcttgccgt
     7861 tttggcggcg aagaattcct ggtgctgttg cccaatatgt cgctggccgc tgccttggac
     7921 aaggcggagc agtggcgcca ggcctttgca gcccagacgg tcctgtggga tggctcgccc
     7981 attcgaacca cactctccat tggggtggcc actttccccg
//
LOCUS       DAOSJM010000062         8017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8368_length_8017_cov_3.674328, whole genome shotgun sequence.
ACCESSION   DAOSJM010000062 DAOSJM010000000
VERSION     DAOSJM010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8368_length_8017_cov_3.674328"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKX82_03440"
     CDS             complement(<1..766)
                     /locus_tag="PKX82_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF51333.1"
                     /translation="MTAYAEFHRRSIQDRDGFWAEQAQLVDWKSAPTQVCDYSNPPFA
                     KWFVGGTTNLCHNAVDRHLKDRAGQAALIAVSTETNTERVYTFADLHAEVQRMAAVLK
                     DLGVQKGDRVLIYMPMIAEAAFAMLACARIGAIHSVVFGGFASGSLALRIEDSEPKVI
                     VSADAGSRGGKVVEYKPLLDEAISLSSHKPGAVLLADRKLAAMNIVAGRDHLWGALRE
                     KHLNTVVPCEWVESTHPSYTLYTSGTTGKPKGVQRDT"
     gene            complement(867..1790)
                     /locus_tag="PKX82_03445"
     CDS             complement(867..1790)
                     /locus_tag="PKX82_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOF51334.1"
                     /translation="MQDLNDLIYFAEVVDRGGFAAAGRSLGLPKSRLSRRVAELEDRL
                     GVRLLQRTTRRLSLTAVGEQYHRHCVAMREDAQAADEAVAQARKEPSGTIRVACPVTL
                     AQSTLGPILPEFMARYPQVKIDMRVSNRAVDLVEEGFDVALRVRPTLEDSGSLVVKNF
                     GFTRTILVASPAQLARQGRPVSPVDLDHLDTVSMSSADGRATWQLVGPDGTTHTFHHV
                     PRYVADDLATLKLAVIQGTGLCAIPDYMCRDDIRAGRLEPILPGWAPKPGIFHAVFPS
                     RRGLVPAVRTFLDFLGDAIAKDGIAQLEPER"
     gene            1913..2563
                     /locus_tag="PKX82_03450"
     CDS             1913..2563
                     /locus_tag="PKX82_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOF51335.1"
                     /translation="MKLLHIDSSVLGNNSVSRQLTAEIVAKWRTSHPGTAVDYLDLAI
                     DAPSHLSADSLGFRMAPGAAELTDIQRKENAVSEALVQQFLAADVIVISAPLYNFSIP
                     SQLKAWIDRVAQVGRTFKYTEKGPQGLAGGKTVIVASTRGGVYSTSEGGRAMEHQESY
                     LQTVFGFFGITDVRFVRAEGLAMGAEPKAQAIADAELAITALTSEAANADSARNAA"
     gene            complement(2560..3327)
                     /locus_tag="PKX82_03455"
     CDS             complement(2560..3327)
                     /locus_tag="PKX82_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF51336.1"
                     /translation="MNELFSLKGRTALITGGSRGIGRMIAEGFLAQGARVYISARKAS
                     ACDATAAELSARGPCFSLPADVSTVEGAQALVAAYSQREESLDILVNNAGAAWGAPFD
                     DFPESGWDKVVDLNLKAPFFLTQALHGHLQKAGKSRVAKVINIASIDGVSVNPMETYS
                     YAASKAGLIHLTRRMALRLVKDNIVVSAIAPGAFASEMNKDARDHEVEVSARIPSGRI
                     GTVEDMAGAAIFLASRAGDYVVGSTLVVDGGVTHARS"
     gene            complement(3438..4049)
                     /locus_tag="PKX82_03460"
     CDS             complement(3438..4049)
                     /locus_tag="PKX82_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOF51337.1"
                     /translation="MLLELEASQLVLVDYQLRLMPAMHDGGAVIANAVRLARMAAALQ
                     VPVFVTEQNPSGLGETVLELKAATTDARARVLGKMQFSAVEEGLGEWLRPPTKPVQGN
                     ARSLPKHLQKPAGGAARDTIVLAGCEAHVCLLQTALDLLEDEFDVWVVTDACSSRTER
                     NRDAAFDRLAGAGVELVTTEMVAFEWLRTAEHPQFREIQALIK"
     gene            complement(4089..4784)
                     /locus_tag="PKX82_03465"
     CDS             complement(4089..4784)
                     /locus_tag="PKX82_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF51338.1"
                     /translation="MEAKIRRQQAMVETRRAMVLDAAKVVFAKVGLERASMREIAKQA
                     GYTPGALYAYFSGKQELLAALLDDMLRRLQEVVLLAKAPKGRSEPGVVTKGQAWLAFF
                     ISQPREMELALSLLVGTGTLRVSPEISRALHVQVRKTLDPVGAVLLAMGATPRQVEVE
                     MESLLAHGLGLLLSQDASRLQSSEQSPEALFAGYLQDLERRYQLPAPSDAPAKRVPVA
                     APQVDLFGQDRSS"
     gene            5020..5571
                     /locus_tag="PKX82_03470"
     CDS             5020..5571
                     /locus_tag="PKX82_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOF51339.1"
                     /translation="MRFCLLFFALLLSTVVHATPNTSGDEVTRLLSERGLLDRIGEVG
                     TRVETKASELVVTAMGFLGVPYKRGGNSLETGIDCSGFVRAIYEQTAGLILPRKAEQQ
                     AAATQKIDRTELQPGDLVFFNTMRRAFSHVGIYVGNGKFIHAPKPGAEVRVEDMGIAY
                     WNRRFDGARRVSVDPNAAASTQP"
     gene            5600..6856
                     /locus_tag="PKX82_03475"
     CDS             5600..6856
                     /locus_tag="PKX82_03475"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF51340.1"
                     /translation="MLFTELGLTPTVLKALAPLGHTSPTSVQAEAIPQAIQGRDVLAL
                     AQTGSGKTLAFALPLLQNLHPSTAGKPRLPQALVLVPTRELAIQVGETFRQLVQGLPS
                     AVKVSIVFGGVSINPQLMALRGGTDILVATSGRLLDLIAHNGLSLSGINMLVLDEADR
                     MLDLGFSEELQAILRLLPGQRQSLFFSATFPAAVQTLANTLLRDPAHINVVSEPQERP
                     DITQRAIAVDPARRTQLLRHLIVESGWKRVLVFVATKHAAEIVADKLRKVRIHAEPFH
                     GELSQGKRMQVLHDFKANRLNVVVATDMAARGLDIPQLPVVVNYDLPRSADDYTHRIG
                     RTGRAGEAGLAVNFVSATTEAHFRLIEKRHHIAVAREQVTGFEPEEMEPPSPTSAPGG
                     GIKGKRPSKKDKLRAALTAGTSRSAS"
     gene            complement(6898..>8017)
                     /locus_tag="PKX82_03480"
     CDS             complement(6898..>8017)
                     /locus_tag="PKX82_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOF51341.1"
                     /translation="PSGAEAEGWLISVGGTPKEMLSRSPEFTYRRLLHAAKIAEKMGA
                     QIMGLGAFTKVVGDAGVTVARRSSLPITTGNSYSASGALWAAADAMRRMGLVHLNKHN
                     KKVAAKTMVIGASGSIGSVSARLLAMSFDEVYLAGRTLSKLDELKASILKETPDAKVF
                     TTIDYNDLLSDMDMIVTSTSGAGKEILDIMRVKPGCVITDVARPLDLPAEDVAKRPDV
                     LVIESGEIDLPTKVKGLKSIGLPPNVVYACLAETIVLALEGRFEVFTVGRDTEWEKVK
                     EIYKLGLKHGMKLAAISGVNGVFSDDDIAKVVKLAKKARLTWKGTGGPTEVAKAPAKK
                     APAKKTAAVKAPAVKAPTKKTVAAKKVASKKTTARKAA"
BASE COUNT         1656 a         2533 c         2225 g         1603 t
ORIGIN      
        1 ccgtgtcgcg ctgcacaccc ttgggttttc cagtcgtgcc gctggtgtac agcgtgtaac
       61 tggggtgggt ggattcaacc cactcgcatg gcaccacggt gttcaggtgt ttctcgcgca
      121 gagcccccca caaatggtca cgtcccgcta ctatattcat agcggcgagt tttcgatcgg
      181 ccagcagtac ggcgccaggc ttgtggctgg agagactgat agcctcgtcc agcagtggct
      241 tgtattccac caccttgccg ccgcgagagc ctgcgtcggc gctgacgatg actttgggtt
      301 cggaatcttc aatacgcaag gccagtgagc cggacgcaaa accgccaaat accaccgagt
      361 ggatggcacc aatacgggcg cacgccagca tggcaaaggc tgcctcagcg atcatgggca
      421 tgtaaatcag gacacggtcg cccttttgca cgcccagatc cttgagtacc gcagccatac
      481 gctgcacttc ggcatgcagg tcggcaaagg tgtaaacccg ctcggtattg gtttctgtcg
      541 atacggcaat cagggctgcc tgaccagccc ggtctttcag atggcggtcc accgcgttgt
      601 ggcacaggtt ggttgttccg ccaacaaacc acttggcaaa cggcggattg ctgtagtcgc
      661 agacctgcgt tggtgcagac ttccaatcca ccaattgggc ttgctcagcc caaaacccgt
      721 ctctgtcctg gattgagcga cgatgaaatt ctgcatacgc ggtcatgtta tgtctccgtt
      781 cgggccgata aaactgaatt cataattatg gataagcaac ttgctgcaag ctgacttcgt
      841 tcgcccaaac ctgccgatca accggttcag cgttctggtt ccagttgtgc gatgccatcc
      901 ttggcaatcg catcccccag aaaatcaagg aacgtccgca cggcgggaac cagccctcgt
      961 ctggatggaa aaactgcatg gaatatgcca ggcttcggcg cccaccccgg aagtatgggt
     1021 tccaggcgcc cggcgcgaat gtcgtcccgg cacatgtagt ccggtatggc gcataggccc
     1081 gttccctgaa tcacggccag tttgagggtc gccaggtcgt cggcgacgta gcgcgggaca
     1141 tggtgaaacg tgtgcgtggt tccgtctggc cccaccagtt gccacgtggc acggccatca
     1201 gcactggaca tgctgaccgt atccaggtga tccaggtcaa cgggagaaac tggacgacct
     1261 tgtcgcgcca attgggccgg actggcaacc aatatggtgc gggtaaagcc aaagttcttg
     1321 accacgaggc tgccactgtc ctccagtgtc gggcgtaccc gcagggccac gtcaaagccc
     1381 tcctccacca gatccacagc ccgattgctc acgcgcatat cgatcttcac ctggggatac
     1441 cgtgccatga actcgggaag aatggggccc aaggtacttt gcgccagcgt taccggacac
     1501 gccacgcgga tggtgcccga cggttctttt cgcgcctgcg ccacggcctc gtccgcagcc
     1561 tgcgcatctt cgcgcatggc aacgcagtgc cggtggtact gctctcccac cgcggtgagc
     1621 gatagcctgc gtgtcgtccg ctgcagcaac cgcacgccca gccgatcttc cagctccgcc
     1681 acgcgccgtg acagcctgga cttgggcaac cccagcgagc gtccggcagc cgcaaatccc
     1741 ccgcggtcca ccacctcggc aaaatagatc aagtcgttga gatcttgcat actcctatga
     1801 ttgtcctaca tgtagaacaa taaagtccaa ttttgctgac ttatcaagaa ttgaactcat
     1861 aggcaaactc tgtccatggc tgcaatttgc agtaatcacc aaggaccgca ccatgaaact
     1921 actccacatc gactccagcg tactgggtaa caactccgtc tcgcgccaac tgaccgccga
     1981 aatcgttgcc aaatggcgta ccagccaccc cggtacagct gtggactacc tggatctggc
     2041 catcgacgcc cccagccatc tgtctgccga ttcgctcgga tttcgcatgg ctccgggtgc
     2101 ggcggaattg acggacatcc aacgcaagga aaatgccgtt tcagaagctt tggtgcagca
     2161 attcctggcc gcggacgtga tcgtgatcag cgcaccgctg tacaactttt cgatccccag
     2221 ccagctcaag gcctggattg accgtgtagc ccaggtgggc cgtaccttca agtacaccga
     2281 gaagggcccg caaggtctgg caggtggaaa gaccgtgatt gtggcgtcca cccgcggcgg
     2341 ggtgtattcc accagcgagg gcggacgcgc catggaacac caggaaagct atttgcaaac
     2401 cgtatttggc ttctttggca tcacggatgt gcgctttgtg cgtgcggaag gactggcaat
     2461 gggcgcggag cccaaagcac aagccatcgc agacgccgag ctggccatta cggcgctcac
     2521 cagtgaagcg gcgaacgccg acagcgcgcg caacgccgcc tagctgcgtg cgtgggtgac
     2581 gccgccgtcg accaccagcg tcgagcccac cacgtagtca ccggcgcgcg atgccagaaa
     2641 gatggcagcg ccggccatgt cctcgacggt gccgatccga cccgaaggaa tgcgcgcgga
     2701 cacctcaacc tcgtggtcac gcgcatcctt gttcatttcc gaagcaaagg ctcccggtgc
     2761 aatcgccgag acaacgatgt tgtctttcac cagacgcaaa gccatgcggc gtgtcaggtg
     2821 gatgagaccc gctttgctgg cagcatacga ataggtttcc atcgggttga ccgacacacc
     2881 gtcaatagag gcaatgttga tcaccttcgc caccctggac ttcccggcct tctgcaggtg
     2941 gccgtgcaaa gcctgggtca gaaaaaaggg cgctttgaga ttcaggtcga ccactttgtc
     3001 ccagccactc tcggggaaat cgtcaaaggg agccccccac gcggcaccgg cgttgttcac
     3061 cagaatatcc agagactctt cgcgctggga gtaggccgcc acgagcgcct gggctccctc
     3121 cacggtcgac acatcagcgg gcagtgaaaa gcacgggcct cgcgcggaca actccgcagc
     3181 agtcgcatcg caggcactgg ccttgcgtgc gctgatatag accctggcgc cctgggcgag
     3241 aaagccctcg gcaatcatgc ggccgatacc gcgggaaccg ccggtaatca gtgcggtacg
     3301 tcctttgagg gaaaacagtt cgttcacgtg cttgctcctg tgcaattgct gtattgagat
     3361 ggagcaagct taggggcaga tgaacagaac gggcgtcgcc aaggtgctag cccagcgccc
     3421 tgccaggcac atccaaccta tttgatcagc gcctgaattt cacgaaattg cgggtgctca
     3481 gcagtacgca accattcgaa cgccaccatt tcggtggtca ccaactccac accggcgccc
     3541 gccagacggt caaacgcagc atcgcggttg cgttctgtgc gggagctgca ggcgtcagtc
     3601 accacccaga catcaaactc atcctccagc aaatcgagtg cggtctgcag caggcagaca
     3661 tgggcctcgc atccggccag aacgatggtg tcacgcgcag caccgccggc cggcttctgc
     3721 aggtgttttg gaagactgcg tgcattgccc tgcaccggct tggtcggtgg gcgcagccat
     3781 tcacccaacc cctcttccac cgcgctgaac tgcatcttcc ccagaacacg cgccctggcg
     3841 tccgtggttg ccgccttgag ttccaatacc gtttccccca aaccagaggg attttgctcg
     3901 gtcacaaaca caggcacctg caaagcagcc gccatgcgtg ccagccgcac cgcattggca
     3961 atcaccgctc caccatcgtg catggcaggc atcagacgca actgataatc caccagtacc
     4021 agttgggaag cttccagttc aagcaacata ggtctctttc tataagttgg cagggagcat
     4081 acccaatctt aagatgaccg gtcctgacca aacaaatcca cttgcggtgc cgctacgggt
     4141 acgcgtttgg ccggtgcatc actgggcgca ggcaactggt agcgacgctc aagatcctgg
     4201 aggtagcccg caaaaagagc ctccggcgac tgctcggagg actggagccg gctggcgtcc
     4261 tgcgacaaaa gcaggcccag gccgtgcgcc agaagtgact ccatctccac ctccacctgc
     4321 cgcggcgtgg cccccatggc aagcaacacc gccccgacgg gatccagggt cttgcggacc
     4381 tgaacatgca aggcccggct gatctctggt gaaacccgca gggtaccggt ccccacaagc
     4441 agcgacaacg ccaactccat ttcacgcggc tgcgagatga agaaagccag ccaggcttgc
     4501 cctttggtca ccacgcccgg ttctgaccgc cccttggggg ctttggccag caggacaact
     4561 tcctgcaggc gcctgagcat gtcatccagc aaagccgcca gcaactcctg tttgcccgaa
     4621 aagtaggcat acaaagcacc gggcgtatag cccgcctgtt tggcgatttc gcgcatgctg
     4681 gcacgctcca ggccaacttt tgcaaacacc accttggcgg cgtcaaggac cattgccctg
     4741 cgtgtctcca ccatggcctg ttgccgccgg attttcgctt ccatgaaata ccttcgtttt
     4801 attttcacaa tattgtacga atcaatttat ttatcatttt tttgaattga caacgtttat
     4861 attgaacatc gtttattatt ttcaattcaa ggaaattcga aaccaattgc aaaccgatct
     4921 gccaaaaata gaaaaattca tttaaatcaa taagttaagt gataaatctc aagttctttc
     4981 ttgcttgacg aagtgggtac ttgtcgatag actcgcccga tgcgtttttg cttactgttt
     5041 ttcgcccttc tgctgtcgac cgtggttcat gccaccccca atacatcggg agacgaggtg
     5101 acccgccttt tgtccgagcg cggattgctg gatcgcatcg gcgaagttgg tacccgggtc
     5161 gaaaccaagg catccgaact ggtggtcacc gccatgggat ttctcggtgt tccgtacaag
     5221 cgtggtggca acagcctgga gaccggcatt gattgcagcg gttttgttcg cgccatttac
     5281 gagcagacag ccggactgat cttgccccgc aaggccgaac agcaggccgc cgccacccaa
     5341 aaaatcgacc ggaccgaatt gcaaccaggt gacctggttt tcttcaacac catgcgccgg
     5401 gcattcagcc atgtgggcat ttatgtcgga aatggcaaat tcatccacgc gcccaaacca
     5461 ggtgccgaag ttcgcgtgga agacatggga atcgcctact ggaaccgccg ttttgatggc
     5521 gcacgccgtg tcagtgtgga tcccaatgct gccgcctcaa cgcagccctg atccgaaaac
     5581 tggcctctct ctctctcgca tgctctttac agaactgggc ctgacgccca ccgtcctgaa
     5641 ggctttggcg cccttgggac ataccagccc caccagcgtt caggctgagg ccattccaca
     5701 ggcaattcaa ggacgtgacg tactggcgtt ggcacaaacc ggctccggca aaacgctggc
     5761 ctttgcactg ccgcttctcc agaacctgca cccaagcacc gctggcaagc cccgactgcc
     5821 acaggccctc gtcctggtgc ccacgcgtga acttgccatc caggtcggtg agaccttccg
     5881 acaactggtg cagggtttgc caagcgcggt caaggtaagc atcgtttttg gcggggtttc
     5941 catcaatccc caactgatgg cgctgcgcgg ggggacggac attctcgttg ccacgagcgg
     6001 gcgactgctg gacctgatcg cgcacaacgg gctgagtctt tcgggcatca acatgctggt
     6061 cctcgacgaa gcagaccgga tgctggacct cggattctcc gaagaactgc aagccattct
     6121 ccgcttgctg cccggccagc ggcaaagcct gtttttctca gccacatttc ctgccgcggt
     6181 tcagacactg gcaaacaccc tgttgcggga cccggcacat atcaacgtcg tctccgaacc
     6241 acaggaacgc ccggacatca cccagcgcgc catcgcggtg gacccggcac gtcgtaccca
     6301 actgctgcgc cacctcatcg tggaatccgg atggaagcgt gttctggttt tcgtcgccac
     6361 caagcacgcg gctgaaatcg tggcggacaa attgcgcaaa gtgcgaatcc atgcagaacc
     6421 gttccatggc gaactcagcc agggtaagcg catgcaggtt ctgcacgact tcaaggccaa
     6481 tcgcctcaac gtcgtggtag ccacggacat ggccgcacgt ggtctggata ttccgcagtt
     6541 gccggtggtc gtgaattacg acctgccccg ctctgccgac gattacacgc accgcatcgg
     6601 ccgaaccgga cgcgccgggg aagcaggcct ggcggtgaac tttgtcagtg ccaccactga
     6661 ggcccacttc cggctgatcg agaagcgcca ccacattgcc gtggcgcgcg agcaggttac
     6721 aggcttcgaa ccggaagaga tggagccacc gtcccccacc agcgcacccg gcgggggcat
     6781 caagggcaaa cgccccagca agaaagacaa gctgcgcgcc gccctgactg ccggcaccag
     6841 ccggtccgcc agctaagtca gggcgtcatt accagacgcc ctgggcaact tgcagactca
     6901 ggcggccttg cgagcagtcg tttttttcga agcgaccttt tttgcggcaa cggtcttctt
     6961 ggtgggtgct tttactgcgg gcgcttttac cgcagcagtt ttcttcgcag gcgccttctt
     7021 tgccggagct ttggcgacct ctgtcggacc acccgtcccc ttccaggtca gtcgtgcctt
     7081 cttggccagt ttgaccacct tggcaatatc gtcgtcgctg aacacgccgt tgacacccga
     7141 gatggccgcc agtttcatgc cgtgtttgag accgagtttg tagatctcct tgaccttctc
     7201 ccactcggtg tcccgtccta cggtaaacac ctcaaagcgg ccttccagcg ccagcacaat
     7261 agtctcggcc aggcaggcgt agaccacatt gggcggcagt ccaatactct tgagtccctt
     7321 gaccttggta ggcaggtcga tctcacccga ctcgatcacc agcacgtccg gccgcttggc
     7381 cacgtcttcg gcgggaaggt ccaacgggcg ggccacgtcg gtaatcacgc aacccggctt
     7441 gacccgcatg atgtccagaa tttccttgcc ggctccggac gtggaggtca cgatcatgtc
     7501 catgtcggac agcaggtcgt tgtagtcaat cgtggtgaac accttggcat ccggtgtctc
     7561 tttcaggatg gaggccttga gttcgtccag cttgctcagg gtccggcccg ccaggtacac
     7621 ctcgtcgaac gacatcgcca gcaggcgcgc gctgaccgaa ccgatggaac ccgaggcgcc
     7681 aatcaccatg gtttttgccg ccaccttctt gttgtgcttg ttcaggtgca ccagccccat
     7741 gcgacgcatg gcgtcggcgg cggcccacag ggcacctgac gccgaatagc tgttgccggt
     7801 ggtgatgggc aggctggagc gccgtgcgac ggtgacaccg gcatcgccca ccactttggt
     7861 gaaggcgccc agtcccatga tctgcgcacc cattttttcg gcaatcttgg ccgcatgcag
     7921 cagacgccgg taggtgaact ccgggctgcg cgacagcatt tctttgggcg taccacccac
     7981 cgaaatgagc cagccttccg cctcggcacc cgaggga
//
LOCUS       DAOSJM010000063         8007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8376_length_8007_cov_3.978496, whole genome shotgun sequence.
ACCESSION   DAOSJM010000063 DAOSJM010000000
VERSION     DAOSJM010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8376_length_8007_cov_3.978496"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="PKX82_03485"
     CDS             complement(<1..880)
                     /locus_tag="PKX82_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF51342.1"
                     /translation="MNTVVDTKSIQDMDSAHFLHPFTDFKDLNGRGARVITKAEGVYV
                     WDSEGKRMLDAMSGLWCVNVGYGRKELAEAAYQQMLTLPYYNSFFQTTNVPAVRLAAK
                     LASLAPAVGDRSFKQVFYSSSGSESNDSNVRMVRRYWDLLGQPQRKVIISRNNAYHGS
                     TMAGASLGGMSGMHAQGDLPIPNITHIEQPYYFDNAEPGETPEAFGVRAAGWLEEKIL
                     AVGPDKVAAFIAEPVQGAGGVIIPPSTYWPEIQRIVDKYGILLISDEVICAFGRLGHW
                     FGYEKFGYKPDLVTFAK"
     gene            complement(925..2292)
                     /locus_tag="PKX82_03490"
     CDS             complement(925..2292)
                     /locus_tag="PKX82_03490"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOF51343.1"
                     /translation="MSVPKTMTFNDLEQWLNERRVTEVECLVPDLTGVARGKILPRAK
                     FTEDRGMRLPQAIVAMGVTGEFPESGPYYDVIDPTDKDMQLRPDPSSVRIVPWAVDPT
                     AQVIHDCFDHQGNLVPFAPRSVLRRVCDLFAAEGLQPIVAPELEFYLTARNTDPNTLL
                     RPPVGRSGRAETSRQAYSIDAVNEFDPLFEEIYDYSDKMELNVDTLIHEVGAGQMEIN
                     FFHAQPLGLADEVFFFKRTVREAALRHDMYATFMAKPIAGEPGSAMHVHQSLVDVATG
                     KNVFSNEDGTPSEMFMHYIGGLQRYIPAAMVLVAPYVNSYRRLSRNTAAPINIEWGYD
                     NRTVGIRSPISSPAARRVENRVIGADANPYVAMAMTLACGYLGIKNKIKPKPEMKGDA
                     YLSPYSLPRSLGEALDWLRKESDLHEVLGKEFITVYSEIKELEFDEFMKVISPWEREH
                     LLLHV"
     gene            complement(2325..3101)
                     /locus_tag="PKX82_03495"
     CDS             complement(2325..3101)
                     /locus_tag="PKX82_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOF51344.1"
                     /translation="MKKPPSLVWLPADHRLLGSEGKTMPFLVLGDKYARAIKVHAQAQ
                     PVMFPLASADQIEDLLALVDGVMLTGSPSNVHPSHFGAAVADTSLPLDEERDSLTLAL
                     VKACVAHAVPLLGICRGFQEINVALGGTLHQQVHAIAGMQDHRESPESSYDVQYAPSH
                     AIRLASGSAFAQWAGGETAHVNSVHGQGIDRLAQGLRALAYAPDGLVEAFEVEGAAAF
                     AYAVQWHPEWKTSESAFYGAIFMAFGDACRNRQANRTSMN"
     gene            3247..4722
                     /locus_tag="PKX82_03500"
     CDS             3247..4722
                     /locus_tag="PKX82_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOF51345.1"
                     /translation="MLSELLLTDMARLSQQDNMPLHRQLYEAMRRAMLDGKLAAGDRL
                     PSSRELTHDLGLSRNTVVAALNQLSVEGYLVSRVGSGTFVHDNVPRSPQRKASSQVPR
                     HQTDLSRRGIALSSTFCATQLEVQPFTPGIADFSAFPVALWQRLQNKHWRMTYPEMLD
                     YSYCGGHAPLRRAVADYLRVFRSVPLDVDQVIITSGTQQSLELCAQLLADHGDTVWLE
                     DPAYWGAVKAFMATGLRAHPVKVDQHGMAPTPQDERTPPRLIYTTPSHQYPTGAVMSL
                     ARRQHILAIARTHNAWVLEDDYDSEFRFSGPPLSSLAGLDQDERVLYMGSFSKVLYPG
                     LKLGYLVVPKRLVGAFKQAHYDLNRPGQMPLQAALAEFIELGHFASALRRARHSYAQR
                     RQCLLEALAPCLGKNAQITGAEQGLHLCVRLPDALDDKALAQRIGTFGLTVRPLSAYC
                     LERQDAKGLVIGYGYAPLAEIERWGPVLSKALLSELKRFQR"
     gene            complement(4733..6034)
                     /locus_tag="PKX82_03505"
     CDS             complement(4733..6034)
                     /locus_tag="PKX82_03505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOF51346.1"
                     /translation="MLLDSDTQLNQKSYYEASVQREPLGEPLRGDVSADVLVVGAGFA
                     GLSSAIELAQRGYKVVVLEADRVCSGASGRNGGQAIVGYASGQEPFEKQLGPAAARQA
                     WDMSLEAIDLIDARIAEFQIDCDRVNGYLYVADSARKARALEVDMKGMERDYGLACDF
                     ATGADVQRHIQSPLYCAAAYEKKSGHLHPLKFGLGLARAARSLGVQIFEKSPVTALQR
                     GATLCATTPAGRVTAKFGVLAGNCMLGEYGPKVAAEITPRIMPVGTYIIGTAPLDPAL
                     CARLIPSNAAVCDNNFVLDYFRFSADHRMLFGGRVSYSARTPARLQQIMARRMGEVFP
                     VLASAPVDYVWGGFVDISMNRAPDFGRLGDNLYYLQGFSGHGVALTGLAGKVVAQAVA
                     GQAERFDVFARLQHRKFPGGSLLRTPSLVIGMAYHQLRDRL"
     gene            complement(6055..6975)
                     /gene="speB"
                     /locus_tag="PKX82_03510"
     CDS             complement(6055..6975)
                     /gene="speB"
                     /locus_tag="PKX82_03510"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOF51347.1"
                     /translation="MAIGQFAYANHSNRFLAAPALENHAFAIAGVPFDGAVTNRPGAR
                     FAPQEIRRASLMLCDGIHPVFNVTPLGVLGDAADMLLPNASPLTEVRKVIEQQAAALM
                     AAHHCVFLGGDHSVTLPLLRAAKARHGKLALIHFDAHCDTWQDHFGEPSGHGTWTYEA
                     IQEGLVDPKQTVQIGLRSSGERAAREYVKDQGGLIYTARDLRGRDGTGLAATISEIRA
                     RLGNTPCYLTLDIDCLDPAFAPGTGTPEPGGMSSSQVLTFLEELAALNFIGMDCVEVA
                     PAYDHAELTSNAAATFVWTYLCGQVAKQAR"
     gene            7171..>8007
                     /locus_tag="PKX82_03515"
     CDS             7171..>8007
                     /locus_tag="PKX82_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF51348.1"
                     /translation="MAYGFLALSMSLVGSYVALSKPLVAALPVFLLAWLRFGIGGLAM
                     AHWLKRPSDEVPMTPQIRKLVFLESLLGNFLFSICMLFGVSMTSAVSAGVIMAAIPAV
                     VALMSWIFLKERIGPRIWAAVVCSALGISLLALSKNELSAQSGRGLEADLAQNKALLG
                     NLLVFAAVLCEAAYAVIGKKLTGALSPKRITALINLWGFALMTPMGLYVAWSFDFSAV
                     PMPSWLLLVFYALAASVWTVWLWMTGLKSVPAAHAGVFTVMLPLSAALVGVLVLGEQL
                     GGV"
BASE COUNT         1584 a         2548 c         2295 g         1580 t
ORIGIN      
        1 ctttggcaaa cgtcaccaga tcgggcttgt agccaaattt ctcgtaacca aaccaatgtc
       61 ccaaacgacc gaacgcacaa atcacttcat cgctgatcaa caagatgccg tacttgtcaa
      121 caatgcgctg aatctcgggc cagtaagtgg acgggggaat gatcactcca cccgcaccct
      181 gtaccggctc tgcaataaaa gccgccacct tgtcgggacc caccgccaag attttttcct
      241 ccagccagcc cgcagcgcgc acaccaaacg cttctggcgt ttcacccggc tcggcattgt
      301 caaagtaata cggttgctcg atgtgggtga tattgggaat gggcaaatca ccttgcgcat
      361 gcatcccgct catcccaccc agggacgcgc cggccatggt gctgccgtgg taagcgttgt
      421 tgcggctgat gatgaccttg cgctgcggtt gtcccagcaa gtcccaatag cgacgcacca
      481 tgcgcacgtt ggaatcattg ctctcgctgc cactggacga gtaaaacact tgcttgaagc
      541 tgcgatcacc cactgcaggt gccagactcg ccagcttggc agccagtctg accgccggaa
      601 cattggtggt ctgaaaaaag ctgttgtagt agggcaatgt gagcatctgc tgataggcag
      661 cttctgccag ttccttacgc ccgtagccca cgttcacgca ccacaagccg ctcatggcgt
      721 cgagcatgcg tttgccttca gagtcccaca cgtagacccc ttcggccttg gtaatcacgc
      781 gcgcaccacg gccattgagg tccttgaaat ccgtgaatgg atgcagaaaa tgcgctgaat
      841 ccatgtcctg gatgcttttg gtatcgacaa ctgtgttcat acaaatgttc cgaatgcgtg
      901 tagtgaaata aaacgttcaa acgttcaaac gtgcagcagc aaatgctcac gttcccaggg
      961 cgagatgact ttcatgaatt cgtcaaactc cagctccttg atctccgaat agaccgtgat
     1021 gaactccttg cccaggactt catgcaggtc cgactccttg cgcaaccagt ccagcgcttc
     1081 acccagactg cggggcaagg aatatggtga cagataggca tcgcccttca tctcgggctt
     1141 gggtttgatc ttgttcttga tacccagata gccgcaggcc agcgtcatcg ccatggccac
     1201 atacggattg gcatccgcac cgatcacgcg gttttccacg cggcgtgctg caggagaaga
     1261 gataggagac cggataccca cagtccggtt gtcatagccc cactcgatgt tgatgggcgc
     1321 cgcggtgttg cgcgaaagcc ggcggtaact gttcacatac ggtgctacca gcaccatggc
     1381 cgcagggatg taacgctgca agccaccgat gtagtgcatg aacatttcgg acggggttcc
     1441 gtcctcattg ctgaacacgt tcttgcccgt tgccacgtct accaggctct ggtgcacgtg
     1501 catggcactt ccaggctcac cggcaatcgg cttggccatg aaggtggcat acatgtcatg
     1561 gcgcaaggcg gcttcacgta ccgtgcgctt gaagaagaac acttcgtctg ccagtcccag
     1621 tggttgtgca tggaagaagt tgatttccat ctgtcctgca cccacctcgt ggatcagcgt
     1681 gtcgacattc aactccatct tgtcgctgta gtcgtagatc tcttcaaaca acggatcgaa
     1741 ctcgttcacc gcgtcaatgc tgtaggcctg ccgcgatgtt tcggcgcgcc cgctgcgacc
     1801 caccggaggg cgcaacaagg tattgggatc ggtattgcgt gcggtcaggt agaactccag
     1861 ctccggcgca acgatgggct gcaaaccttc cgcagcaaac agatcgcaaa cccgacgcag
     1921 cacgctgcgt ggggcaaatg gaaccagatt cccctgatga tcaaaacaat catggatcac
     1981 ctgtgcggtg ggatccacag cccacggaac gatgcgcacc gacgatgggt ctggacgcaa
     2041 ctgcatgtcc ttgtctgtgg ggtcaatcac gtcgtaatac ggaccgctct cggggaattc
     2101 accggtgacc cccatggcca cgatggcctg tggcagccgc atgccacggt cttccgtgaa
     2161 cttggcccgc ggcagaatct ttccacgcgc cacacccgtc aagtcgggta ccaggcattc
     2221 cacttcggta acccgacgct cgttgagcca ctgctccaga tcattgaaag tcattgtctt
     2281 gggtacactc atcgcacact cctcgctgcg ggcatttctg cccgttagtt cattgatgtt
     2341 ctattggctt gcctgttacg gcacgcatca ccaaatgcca taaagatggc accgtaaaaa
     2401 gcgctctcgg acgtcttcca ttcggggtgc cactggaccg catacgcaaa cgcggccgca
     2461 ccttccacct caaaagcctc gaccaatccg tcaggagcat atgccagagc acgcaatccc
     2521 tgtgccagcc gatcaatacc ctgaccatgc accgaattca cgtgcgccgt ttcaccacca
     2581 gcccactgtg caaaggcaga accggacgcc agccggatgg catggctggg tgcgtactgc
     2641 acatcgtacg aactctcagg cgactcccgg tggtcctgca ttccggcgat ggcatgcacc
     2701 tgctggtgca gcgtaccgcc caaggccaca ttgatttcct gaaaaccgcg gcaaatcccc
     2761 agaagcggca ccgcatgggc tacacaggcc ttgaccagtg caagcgtcag actgtcgcgt
     2821 tcttcatcca gggggagcga ggtatctgct acggctgcac caaaatggga agggtgcacg
     2881 ttggagggcg accccgtcag catgacaccg tccaccagag caagaaggtc ttcaatctga
     2941 tcggccgatg ccaatggaaa catcaccggc tgcgcctgtg catggacctt gatggctctg
     3001 gcgtatttgt cacccagaac caggaacggc atggtttttc cctcactgcc cagcaggcgg
     3061 tgatccgcag gaagccagac cagtgatggc ggctttttca tagatgtctc cgtgttcttc
     3121 gctgtcattg tgaggcagaa ttggttccaa ttaagagcca tttgcaccaa ctggaaggtg
     3181 ccagtttaaa acctggcacg acaccctctt ccatcatccg tatcttcact ggaacccgtt
     3241 cacgccatgc tctccgaact actgcttacc gacatggcgc gcctgagcca gcaggacaac
     3301 atgcccctgc accgacagct ctacgaggcc atgcggcgcg ccatgctgga cggaaaactg
     3361 gcggctggcg accgtctgcc gtcgagccgg gaactgaccc atgatctggg gctgtcgcgc
     3421 aacacggtcg tggcggcgct gaaccagctc agcgtcgagg gctacctcgt cagccgtgtc
     3481 ggcagcggca ccttcgttca tgacaacgtt ccccgctccc cgcagcgcaa ggcatccagc
     3541 caggttccgc ggcaccagac agacctgtcc aggcgcggca tcgcactgtc ctccacgttc
     3601 tgcgcaacac agctggaagt tcagcccttc acaccaggga ttgcggactt cagtgcattc
     3661 ccggtggcgt tgtggcaacg gctgcagaac aagcactggc gcatgaccta cccggagatg
     3721 ctggactaca gctattgcgg cgggcacgcg ccgttgcgcc gcgcggtggc ggactacctg
     3781 cgtgtgtttc gcagcgtacc gctggatgtg gaccaggtca tcatcaccag tggtacgcag
     3841 cagtcgctcg aactgtgcgc acaattgctg gccgatcacg gcgacaccgt gtggctcgaa
     3901 gaccccgcct actggggtgc cgtcaaggca ttcatggcca ccggcctgcg tgcccacccc
     3961 gtcaaggtcg atcaacatgg catggctcca acaccgcagg atgaacgcac accaccccgc
     4021 ctgatttaca ccacaccttc gcaccagtac cccaccgggg ctgtcatgtc gctggcgcgc
     4081 cgccagcata ttctggcgat tgcacgcaca cacaacgcct gggttctgga ggatgactat
     4141 gacagcgagt tccgcttcag tggtccgccc ctgtcctcgc tggccggact ggaccaggat
     4201 gaacgcgtgc tgtacatggg atcgttttcc aaagtgctgt acccggggtt gaagctcggt
     4261 tatctggtcg ttcccaaacg cctggtgggt gccttcaaac aggcgcacta cgacctcaac
     4321 cgccctggac aaatgcctct gcaggccgcg ctggcagagt tcatcgaact gggacatttc
     4381 gccagcgcac tgcgcagggc gcgtcacagc tatgcgcagc gacgccagtg cctgctggaa
     4441 gctctggcac cgtgtctggg aaaaaacgcc cagatcaccg gagcagaaca gggcttgcac
     4501 ctgtgcgtgc gactgcccga tgcgctcgac gacaaagccc tcgcccaacg cattggcacc
     4561 ttcggtctga cggtgcggcc actgtcagcc tattgcctgg agcggcagga cgccaaaggt
     4621 ctggtcattg gttacggtta tgccccattg gcagaaattg aacgctgggg cccggtgctg
     4681 tcgaaggcac tgctttccga actcaaacgc ttccagcgtt aaaccgcgcg gatcagagtc
     4741 ggtccctcaa ctggtggtag gccatgccaa tcaccagact gggcgtgcgc agcagcgacc
     4801 cgccgggaaa cttgcggtgc tgcaggcgcg cgaacacatc aaagcgctct gcctggccag
     4861 ccacagcctg cgcgactacc ttgcccgcca gtcccgtcag ggccacaccg tgcccgctaa
     4921 aaccctgcag gtaataaagg ttatccccca gacgcccgaa gtccggcgcg cggttcatgc
     4981 tgatatccac aaacccgccc cacacatagt ccaccggtgc actggccagc acaggaaaca
     5041 cctcccccat gcgccgcgcc atgatttgct gcaaccgggc cggcgtgcgc gccgagtagc
     5101 tgacacgccc gccaaacagc atgcggtggt cggcactgaa acggaaatag tccagcacaa
     5161 agttgttgtc gcacaccgcc gcgttactgg gaatcaggcg cgcgcacaag gccgggtcca
     5221 acggtgccgt gccaatgatg taggtgccca ccggcatgat gcgcggggta atttcagcag
     5281 ccactttggg cccgtattca cccagcatgc agttgccggc cagcacgccg aacttcgcgg
     5341 tcacacgccc ggccggtgtc gtggcacaga gcgtcgcgcc gcgctgcagc gcagtaacgg
     5401 gggacttctc aaaaatctgc acccccagcg aacgtgccgc acgcgccagg cccagcccaa
     5461 acttcagggg gtgcaggtgc cccgattttt tttcataggc cgccgcacag tacagcgggc
     5521 tctggatatg gcgctgcaca tccgctcccg tcgcaaagtc gcaggccagt ccataatcgc
     5581 gctccatgcc tttcatgtca acctccaacg cacgcgcctt gcgggctgag tccgccacgt
     5641 acaggtaacc gttgacgcga tcgcaatcga tctggaactc agcaatccgc gcatcgatca
     5701 ggtcgatggc ctccagcgac atgtcccacg cctggcgggc cgcagcgggc cccagctgct
     5761 tttcgaaggg ctcctgcccg ctggcatagc ccacgatggc ctgcccgcca ttgcgccccg
     5821 acgcgccgct gcacacccgg tccgcctcca gcaccacgac cttgtagccc cgttgcgcca
     5881 gttctatggc ggaggacagt ccggcaaaac cggcgccgac caccagcaca tcggccgaca
     5941 cgtcgccgcg cagaggttca cccagcggct cccgctgcac actggcttcg tagtagctct
     6001 tctggttcaa ctgggtatcg gaatcaagca gcatgggggt tctctcgcag cgaatcagcg
     6061 ggcttgtttg gcaacctgtc cacacaggta ggtccagacg aaggtggcgg cggcattact
     6121 ggtcagctcg gcatggtcgt acgcgggggc cacctccacg cagtccatgc cgatgaagtt
     6181 gagtgctgcc agctcttcca gaaacgtcag cacctgcgaa ctgctcatgc cgcccggctc
     6241 gggcgtacca gtgccgggcg caaacgccgg gtccagacag tcgatgtcca gtgtcaggta
     6301 gcagggcgtg ttgcccagcc gggccctgat ctcgctgatg gtggccgcca atcctgtgcc
     6361 atcgcgaccg cgcaggtccc gcgccgtata gatcaggccg ccctggtctt tcacgtattc
     6421 ccgcgcggcc cgctcgccgc tggagcgcaa accgatctgc accgtttgct tggggtccac
     6481 cagtccctcc tgaatcgcct cgtaagtcca ggtaccgtgg cccgacggtt cgccgaaatg
     6541 gtcctgccag gtatcgcagt gggcatcgaa atggatcagt gccagcttgc catgtcgggc
     6601 tttggccgcg cgcagcaaag gcagcgtgac cgaatggtcg ccacccagaa acacgcagtg
     6661 gtgcgctgcc atcagcgcgg cagcctgttg ctcaatcacc ttgcgcacct ccgtcagcgg
     6721 tgaagcgttg ggcagcagca tgtccgcggc atcccccagc acacccaatg gcgtcacgtt
     6781 gaacaccggg tggatgccgt cacacaacat gagggaggcc cggcggattt cctgcggcgc
     6841 aaagcgcgca ccggggcggt tcgtgaccgc gccgtcaaag ggcacgcccg caatggcgaa
     6901 ggcgtggttc tccagggctg gtgcagccag aaaacggttg ctgtggttgg cgtaggcaaa
     6961 ttgtccgatg gccatagatg gagctttctt gaagaagccc tgaaggtacc gcaatccccg
     7021 gccatccgaa ggtgccaatt ccatccgggg ccgtgaaacc actgaagcgc agggccggaa
     7081 tcgtcggtgg gtggtgtacg cttcaccgct ttgcccgcca gatacgcaca ctgcatgcac
     7141 cacgttcccc atctcccacc aaggcgctgg atggcctatg gcttcctggc cctgagcatg
     7201 tcgctggtcg gcagctatgt tgccctgtcc aagccgctgg tcgcagccct gccggtgttt
     7261 ttgctggcct ggctgcgctt tggcatcggt ggtctggcca tggcgcactg gctcaagcgg
     7321 ccgagcgatg aagttccgat gacgccacag atccgcaagc tggtgtttct ggaatcgctg
     7381 ctgggcaact ttctgttttc catctgcatg ctgtttggcg tgagcatgac cagcgccgtc
     7441 tcggctggtg tcatcatggc agccatccct gccgtggtgg ctttgatgag ctggatcttt
     7501 ctcaaggagc gcatcggccc acgcatctgg gcagccgtgg tctgcagcgc gctaggcatc
     7561 agtttgctgg cgctatcaaa aaatgagcta tctgcgcaat ccggacgagg gctggaggcc
     7621 gatttggctc aaaacaaggc gttgctgggc aatctgctgg tatttgccgc ggtgctgtgc
     7681 gaagcggcct acgcggtgat tggcaagaag cttaccggcg ccctgtcgcc caagcgcatc
     7741 actgcgctga tcaacctctg gggctttgca ttgatgaccc cgatgggcct gtatgtggcc
     7801 tggagctttg acttctccgc agtgcctatg ccgtcgtggc tgctgctggt gttctatgcg
     7861 ctggctgcca gcgtgtggac ggtctggctg tggatgaccg ggctgaagag cgtgcccgcg
     7921 gcccatgcgg gtgtctttac cgtgatgctg ccgctctcgg cagcactggt gggggtgctc
     7981 gtgctgggcg agcaactcgg tggagtc
//
LOCUS       DAOSJM010000064         7974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8408_length_7974_cov_4.383129, whole genome shotgun sequence.
ACCESSION   DAOSJM010000064 DAOSJM010000000
VERSION     DAOSJM010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7974
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8408_length_7974_cov_4.383129"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="PKX82_03520"
     CDS             complement(<1..1251)
                     /locus_tag="PKX82_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HOF51349.1"
                     /translation="MQATPLKYASQSVSKYYFVAALALFTAQIVFGLTLGLQYVIGDF
                     LFPYIPFNQARMVHTNLLIVWLLFGFMGAAYYLVPEESETELYSPKLAMVLFWVFLVA
                     GALTIVGYLAVPYAKLAELTGNDLLQTMGREFLEQPLPTKVGIVVVALGFLFNISMTV
                     LKGRKTSISLVLLLGLWGLALMFLFSFVNPHNLVRDKMYWWFVVHLWVEGTWELILGS
                     LLAFVLIKTTGVDREVIDKWLYVIIAMALVTGILGTGHHFFFIGMPGYWLWVGTIFSA
                     MEPIPFFMMTVFAFNMVQRRRREHPNQAAVLWAVGCSVVGFLGAGLWGFMHTLSAINY
                     YTHGTQITAAHGHLAFYGAYVLVVIAIISYAMPILRGREANSRRAQSVEMWSFWIMTI
                     GMAVMVLALTGAGILQVWLQRLPTT"
     gene            complement(1290..1727)
                     /locus_tag="PKX82_03525"
     CDS             complement(1290..1727)
                     /locus_tag="PKX82_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOF51350.1"
                     /translation="MTQAKAGGFTKQMARNMFYGGSLFFILVFAALIFDSERKIPQRS
                     NAEAITPAVIAGKKLWETRNCIGCHTLLGEGAYFAPELGNVYKRRGPEFIKAWIQSQP
                     TQAPGRRQMPQFNFTEQQLNDLVEFLRWTGNINTENWPPNIEG"
     gene            1885..2583
                     /locus_tag="PKX82_03530"
     CDS             1885..2583
                     /locus_tag="PKX82_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF51351.1"
                     /translation="MELEQFNIPRFLAVLPLFSDLSPADLARMAGGCDLKRLNRGDTI
                     FRYGEPCEEFHVVVTGQIKLFALFQAGQEKVIELVGPGHSFAEALMFTSKPYILNAQA
                     LTSTLLLTVGKQAIVAEIERDPRFCMRMLAGISRRLHGLIQDIESGALQNGVQRVIGY
                     LLREYQIEAESPEQAFTVALPVSKATIASRLSLTPEYFSKVLRELEEQGLIAMDKRDI
                     RIIDPQRLATYGVQ"
     gene            complement(2568..3263)
                     /locus_tag="PKX82_03535"
     CDS             complement(2568..3263)
                     /locus_tag="PKX82_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOF51352.1"
                     /translation="MRTTGFLGVGIAILLSACLVQAEVFVPPWCTDQDARHTASSADG
                     QWILWSDAEKPAMTLFDAKPQAIRQYTVATRDGKSPSRIHAIYDAPARRSFIVVLRDI
                     PEIWEISYDPQAEPIFDGLVHDYRMGEGLAHAGFLGVRRTLLQTQLADLYFTDDYRTA
                     VSITAAAHNAPAQAHVINLDVRRELAAVPLATYQPADAAISVAVDGRTWRAMPGLRQN
                     ISAHQALRRYCTP"
     gene            complement(3276..3581)
                     /locus_tag="PKX82_03540"
     CDS             complement(3276..3581)
                     /locus_tag="PKX82_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOF51353.1"
                     /translation="MKTVLIAAAALLAFGAQAAPVDDAMNKAGCMACHAKDKKLVGPS
                     FKDIAAKYKGQDAVAKLMEKVRKGGSGTFGPIPMAPNGPDKINDADLKAAVELILKS"
     gene            complement(3644..5365)
                     /locus_tag="PKX82_03545"
     CDS             complement(3644..5365)
                     /locus_tag="PKX82_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HOF51354.1"
                     /translation="MKTRKLSRVAALALSALALTAFAQDKKGITAGEMNYQAGASPLG
                     SEPMVQSTNPKAPPMTQAEFDVARKIYFERCAGCHGVLRKGATGKPLTPDLTVAKGTD
                     YLKIFIAYGSPAGMPNWQTSGEMTEAQVDLMARYIQHEPPQPPEFGMADMKKTWKVQV
                     APKDRPTKKMNKFNIENIFSTTLRDTGEIALIDGDTKQIINIIKTGYAVHISRMSASG
                     RYLFVIGRDAKINMIDLWMEKPDSVAEVRIGLEARSVETSKAKGYQDKLVIAGAYWPP
                     QFTLMDGDTLEPKKIVATRGMVVGTQEYHPEPRVASILGSHYKPEFIVNIKETGKTLM
                     VDYSNLDALKITEIGSAPFLHDGGLDASKRYFMVAANNSNKIAAIDTKDGKLAGLVDV
                     GKIPHPGRGANFTHPKFGPVWATGHLGDETISLIGTDPKKFKQYAFKEVAKLTGPGGG
                     ALFVKSHPKSNNLWSDAPLNPDPKISQSIVVYDIKNLDKGYKVLPIAEWAGIKADGGA
                     MRVVQPEYNKAGDEVWFAVWSAKNKQSALVVVDDKTLKLKAVIKDPRLITPTGHFNVY
                     NTQHDVY"
     gene            5588..7897
                     /locus_tag="PKX82_03550"
     CDS             5588..7897
                     /locus_tag="PKX82_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HOF51355.1"
                     /translation="MINHMKIPALRTIAVAASMACTGIGNAQTQTYVPVLKEVVVVSG
                     SRNEQLVEDLPLAVDVISAQDLEADQVGDIRDAVRTLPNVSVKRAPARYGVTGRGNSV
                     GADGNAGFNIRGMGGNRVVMLVDGVRLPRSYINGSDAFSRDTVAVGVLKRIEVIHGPA
                     SVLYGSDALAGLVNFITLEPSDYLLNATDSSPKSIGGKAWLSYSGDDQGSTVGGTVAV
                     RANEAMEWSLTGVQTRASALGNMGSNDAANVDRTTPNPQTNNNNAILGKLVLHPSGTQ
                     RHAFTLEHVHKTSEVELLSSRVKTPVNVTSTANAASAVIGESSKKISDRDRGSWEGQY
                     RMDTAWADRLQLVLSAQNSHALDDGYTLLKTAAANDGKRVRIMNYDERTLQGNVQADK
                     VFVLSPQWSHKLTYGLDVVRTDVSSFADGSDPAPLTPYIPKRYFPDMRDSSQAVYVQS
                     EWISDAWSITPGVRYDQFALDVLSQDGYYPGIATGPGKSLSGSAVTPKLGAVFRVSPQ
                     WAVYGNYAAGFRAPEGQQLNSALEVSTAVLLPNPDLKQEESNNFEIGTRVKLDRLSLD
                     FSVFSGVYTNLIVEKKDLGTANGLPASTTNKTRFQTVNIDKATISGFEIRGNYDWGQF
                     ANGRLSSPFGYGFTRGTNDATGLPLNYIEPAKLAFGLKYETSNWDVRLDAVFQDEKKL
                     QDLDSAYIPKSLTQLQFLVPASTTLNLSGQWRIRKDIRMNLAINNLTDRKYWNWSDVQ
                     GLASNASPVVVDAYTQPGRHANVSLVVDF"
BASE COUNT         1690 a         2182 c         2361 g         1741 t
ORIGIN      
        1 tgtggttggc aggcgttgca gccagacttg caggatgcca gcgccagtca gggccagcac
       61 catgacggcc ataccgatgg tcatgatcca gaagctccac atttccacgc tttgggcgcg
      121 gcggctattg gcttcacgtc cacgcaggat gggcatggcg tagctgatga tggcaatcac
      181 caccagaaca taggcaccgt agaaggccag gtggccgtgc gcagcagtga tctgggtacc
      241 gtgggtgtag tagttgatgg cgctcagcgt gtgcatgaag ccccacagac ccgcacccag
      301 gaaaccgacc accgagcagc ccacagccca cagcacggcg gcctggttgg ggtgttcgcg
      361 gcgacgacgt tgcaccatgt tgaaggcaaa caccgtcatc atgaagaaag gaatgggttc
      421 catggccgag aagatcgtgc cgacccacaa ccagtagccg ggcataccga tgaagaagaa
      481 gtggtggccg gtacccagga taccggtaac cagtgccatg gcgatgatca catacagcca
      541 cttgtcgatc acttcgcggt ccacaccggt ggtcttgatc aggacaaaag ccagcaacga
      601 acccaggatc agttcccagg tgccttccac ccagaggtgc accacaaacc accagtacat
      661 cttgtcgcgt accaggttgt gcgggttcac gaagctgaac aggaacatca gtgccaggcc
      721 ccacaggccc aacagcagca ccagcgaaat gctggtcttg cggcccttga gaacggtcat
      781 gctgatgttg aacagaaatc ccagggcaac gaccacgatg cccaccttgg tgggtagcgg
      841 ttgctccagg aactcgcgtc ccatcgtctg cagcagatca ttgccggtca gctcagccag
      901 cttggcatac ggcacggcca gataacccac gatggtgagc gcaccagcga ccagaaacac
      961 ccagaacagc accatggcga gcttggggct gtagagctcg gtctcggact cttcaggcac
     1021 caggtagtag gcggcgccca tgaagccgaa cagcagccag acgatgagca ggttggtgtg
     1081 aaccatgcgc gcctggttga acgggatata cggaaacaga aagtccccga tgacgtactg
     1141 gagtcccagc gtgaggccaa agacgatctg ggccgtgaac aacgccagcg cggcaacgaa
     1201 gtagtacttc gaaaccgact gcgaggcgta cttgagaggg gtcgcttgca ttttgagtgt
     1261 tccttttcgg tttgggcgtg atgagcgctt tagccttcaa tgttcggtgg ccagttctcg
     1321 gtattgatat tcccggtcca gcgcaggaac tcgacgagat cattcaactg ctgttcagtg
     1381 aagttgaact gcggcatctg gcgacggcca ggcgcctgtg tgggctgcga ctggatccat
     1441 gccttgatga attcggggcc gcggcgcttg tacacgttgc ccaactctgg cgcaaaatac
     1501 gcgccttcgc ccagcagcgt gtggcaaccg atacagttgc gtgtctccca caacttcttg
     1561 cctgcgatga cggccggcgt gatggcctcg gcattcgagc gttggggaat ttttcgttcg
     1621 ctatcgaaaa tgagtgcggc aaacaccaat atgaaaaaca gggaaccgcc ataaaacata
     1681 ttgcgggcca tctgcttggt aaagccgcca gcttttgctt gggtcattgc gttactactc
     1741 cagtgatgaa taatctgcca cgattttaga gcgtggggcg tcgtttatcc ttgaacgact
     1801 tcaagaatgc gtaagaaaca gaatttgcct gtgtttcccg cctcttgcca gctctatgca
     1861 ctatgacaat atggaccgtt attgatggaa cttgaacaat tcaacatccc ccgcttcctg
     1921 gcagtgttgc cactttttag cgatttgtcg cctgcagatc tggcccgcat ggccggtggc
     1981 tgtgacctga aacgcctgaa ccgaggtgac accatatttc gctacggcga gccatgtgaa
     2041 gagtttcatg tggtggtgac tggtcagatc aaattgtttg cgctgttcca ggctggacag
     2101 gaaaaagtca ttgaactggt cggaccgggg cacagctttg ccgaggcgct gatgttcacc
     2161 agcaaacctt acatcctcaa tgcccaggcc ttgaccagca cactgctgct gaccgtgggg
     2221 aagcaggcca tcgtggctga gattgaacgg gacccccgct tctgcatgcg catgctggcg
     2281 ggcatttccc gaaggttgca tgggttgatc caggatatcg aatccggcgc tctgcaaaat
     2341 ggcgtgcaaa gggtgattgg ctatttgctg cgtgaatacc agatcgaagc cgaatcacca
     2401 gagcaagctt ttacggtggc gttacccgtg agcaaggcca cgattgcctc acggctttcc
     2461 ctgacgccgg agtatttttc caaagtgctg cgcgagctgg aagagcaggg actgatagca
     2521 atggacaagc gcgatatccg catcatcgat ccacagcgac tggcgaccta cggcgtgcag
     2581 tagcgccgca gggcttgatg ggcggagatg ttctggcgca gccccggcat tgccctccag
     2641 gtgcgtccat cgactgcgac cgaaattgcc gcatcggcag gctggtaggt agccagtggg
     2701 acggcggcca attccctgcg aacatccaga ttgatcacgt gggcttgggc aggcgcgttg
     2761 tgggccgctg cagtaatgct aacggcggtg cggtaatcgt ccgtgaagta cagatccgca
     2821 agctgcgtct gcagcaaagt ccggcgtacg cccagaaaac cggcatgtgc cagtccttcc
     2881 cccatgcggt agtcatggac cagaccgtca aagatgggtt cggcctgcgg gtcataggaa
     2941 atttcccaga tctcgggtat gtcgcgcagc accacaataa agctgcgccg agcgggcgcg
     3001 tcgtagatcg cgtggatccg tgaaggagat tttccatccc gggtggctac cgtgtactgg
     3061 cgaatggcct ggggttttgc atcaaacagg gtcattgccg gtttttctgc atcggaccag
     3121 agaatccact ggccatctgc ggatgaagcc gtgtggcggg cgtcctgatc ggtgcaccag
     3181 ggtggcacaa acacctcggc ctgcacgagg caggccgaga gcagtatggc aatgcctacc
     3241 cccagaaagc cggtggtacg catggtgaag ccaggtcagg acttgaggat caattccacg
     3301 gccgctttca gatcagcgtc gttgatcttg tcagggccgt tcggcgccat gggaatcggg
     3361 ccaaaagtgc cggagccgcc cttgcgaact ttctccatca gtttggcgac cgcatcctgg
     3421 cctttgtatt tggcagcaat gtccttgaaa gacgggccaa ccagtttttt gtccttggcg
     3481 tggcaggcca tgcagccagc cttgttcatg gcatcgtcca ccggggctgc ctgcgcgcca
     3541 aaggccagca gtgcggctgc tgcaataagt acagttttca ttctcgtctt ctccatgttg
     3601 agcggggcca ctggggcccc gcagttagta tcagcgcgta attttaataa acatcgtgct
     3661 gggtgttata cacattgaag tggcctgtcg gtgtgatcag acgggggtcc ttgatcacgg
     3721 ccttgagttt caacgtcttg tcatccacga ccaccagtgc agactgcttg tttttggcgg
     3781 accagaccgc aaaccacact tcgtcgcctg ccttgttgta ctcgggttgt accacgcgca
     3841 tagcaccgcc gtcggctttg ataccggccc attcagcgat aggcagcacc ttgtaaccct
     3901 tgtccaggtt cttgatgtca tacaccacga tggactggga gatcttcgga tcggggttca
     3961 gaggcgcgtc agaccacagg ttgttggact tgggatggct cttgacgaac agagcgccgc
     4021 cgccaggacc cgtcagcttg gccacttcct tgaaagcgta ctgcttgaac ttcttgggat
     4081 cggtgccgat cagcgagatc gtctcgtcgc ccaggtggcc ggtagcccat acggggccga
     4141 acttggggtg cgtgaagttg gcgccacggc cggggtgcgg gatcttgccc acgtccacca
     4201 gaccagccag cttgccgtct ttggtgtcaa tcgcagcaat cttgttgctg ttgttggcag
     4261 ccaccatgaa gtaacgcttg gacgcatcca ggccgccgtc atgcaggaac ggagcagaac
     4321 cgatttcggt gatcttgagg gcgtccaggt tggagtagtc caccatcagc gtcttgccgg
     4381 tttccttgat gttcacgatg aactcgggct tgtagtggga acccagaatg gatgccacgc
     4441 ggggctcagg atggtattcc tgtgtgccca caaccatgcc acgggtggca acgatcttct
     4501 tgggctcaag cgtgtcgccg tccatcaggg tgaactgggg cggccagtag gcgccagcga
     4561 tcaccagctt gtcctggtag cccttggcct tggaggtttc caccgagcgg gcttccagac
     4621 cgatgcgcac ttctgccacg ctgtcaggct tttccatcca caggtcgatc atgttgatct
     4681 tggcgtcacg gccaatcacg aacaggtagc ggcccgaagc cgacatacgc gagatgtgca
     4741 ccgcatagcc ggtcttgatg atgttgatga tttgcttggt gtcgccatcg atcagcgcaa
     4801 tctcacccgt gtcacgcagc gtggtggaga agatgttctc gatgttgaac ttgttcatct
     4861 tcttggtggg gcgatctttg ggcgccacct ggactttcca ggttttcttc atatcggcca
     4921 tgccgaactc gggcggctgt ggtggctcgt gctggatgta gcgagccatc aaatccacct
     4981 gggcttcggt catttcaccg gatgtttgcc aatttggcat accggctggc gagccgtagg
     5041 cgatgaaaat cttcaggtaa tcggtgcctt tggcgaccgt caggtccggc gtcagaggct
     5101 tgccagtggc gcccttgcgc agcacgccgt ggcagccggc acagcgctca aagtaaatct
     5161 tgcgggccac atcgaactcg gcctgcgtca tcggaggtgc cttggggttg gtgctttgca
     5221 ccatgggctc gctacccaga ggggacgcac ctgcctggta attcatttcg ccagcggtga
     5281 tgcccttttt gtcctgcgcg aatgcagtca aagccaaggc ggacagtgcc aaggctgcta
     5341 cacgggacag ctttcgagtt ttcatggatt cttcctttat ttttgcgatg agccctggtt
     5401 gagccattct ggtttcccag agtgatccgc tgcggactct gacaatgtga cttcagcgaa
     5461 tgcaagcatg atcggacaga tgcatgcggc gaacattaat ttggatcaag gttacggtgt
     5521 attcctcagc gttgcttaag ggtttcccct aataatcacg ccagccatca agaagagata
     5581 catgcgcatg atcaaccata tgaaaattcc tgcactccga accattgccg tcgccgcttc
     5641 tatggcgtgt accggcattg gcaacgcaca aacgcaaacc tacgttccag tgctcaagga
     5701 agtcgttgtc gtcagtggtt cccgcaacga acagcttgtt gaagacttgc cactcgccgt
     5761 ggatgtgatc agtgcgcagg accttgaagc agatcaggtg ggtgacattc gggacgcggt
     5821 gaggacattg cccaatgtct ctgtcaagcg cgcacctgcg cgttacggtg ttaccggcag
     5881 ggggaattca gtgggtgccg atggcaacgc ggggttcaac atccgtggca tgggcggcaa
     5941 ccgggtggtc atgctggtgg atggcgtgcg gctgccgcgc agctatatca atggaagcga
     6001 tgctttcagc cgtgacacgg tcgcggtcgg ggtgctcaaa cgcattgaag tcattcatgg
     6061 tccggcatcg gtgctgtatg ggtcggacgc gttggccggt ctggtgaact ttattaccct
     6121 ggagccctct gactacctgc tgaatgcgac cgatagttcg ccaaagtcca ttggcggcaa
     6181 ggcctggttg tcctacagcg gcgatgatca gggcagcact gtgggtggca cggtggccgt
     6241 gcgtgccaat gaagcgatgg agtggtcgct gaccggggtc cagacccgcg ccagcgctct
     6301 gggaaatatg ggtagcaacg atgcggccaa tgtggatcgc actacgccca atccgcaaac
     6361 caacaataac aacgccatct tgggaaaatt ggtgctgcat ccttcaggga cgcagcgcca
     6421 tgccttcacg ctagagcatg ttcacaagac ttcggaggtg gagctgttgt ccagccgggt
     6481 caagactccg gtaaatgtca catcgactgc caacgctgcc agtgcggtga tcggagagtc
     6541 ctccaagaaa atcagtgata gggaccgggg tagctgggag ggtcaatacc gaatggatac
     6601 ggcatgggca gaccggttgc agttggtctt gagtgcccag aacagccacg ccctggacga
     6661 tgggtatacc ctgctcaaga ccgccgccgc caatgatggc aagcgggttc ggatcatgaa
     6721 ctatgacgag cgtacattgc aggggaatgt gcaggcagac aaggtgtttg tgctgtcgcc
     6781 gcagtggtcg cataagttga cctacgggtt ggacgtggta cgtaccgatg tatccagttt
     6841 tgccgatggc agcgacccgg ctccactgac tccctatata cccaagcgtt actttccgga
     6901 tatgcgcgac agcagccagg cggtctatgt gcaaagcgaa tggattagcg atgcctggag
     6961 tattacgccc ggtgtgcgct atgaccagtt tgcactggat gttctgagtc aggatgggta
     7021 ctaccctggt atcgctacag gtcctggaaa atcactttcg ggctctgccg tcacccccaa
     7081 actgggtgcc gtgtttcgtg tcagccccca gtgggcggtg tatggaaatt atgcggcggg
     7141 ttttcgggcg ccagaaggac agcagctcaa cagcgcactg gaagtttcca cggcggtgct
     7201 gctacccaac ccggatttga agcaggaaga aagcaataat tttgaaatcg gcacgcgcgt
     7261 caaactggat cgactgagcc tggatttttc tgttttttca ggtgtttaca ccaacctgat
     7321 tgttgagaaa aaagacctcg gcactgcaaa cggcctgcca gccagtacga ctaacaagac
     7381 gcggttccaa accgtcaata tcgacaaggc gacgatttcc ggtttcgaaa ttcgaggcaa
     7441 ctacgattgg ggccagtttg caaacgggcg tctgagttct cccttcggct atggattcac
     7501 tcgcggaacc aacgatgcaa ctggcttgcc actcaattac attgaaccag ccaagcttgc
     7561 attcgggttg aaatacgaga cctccaactg ggatgtgcgc ctggatgccg tattccagga
     7621 cgagaagaag ttgcaggatc tggatagtgc atatattccc aaatccctta ctcaactgca
     7681 gtttctggtg cccgcttcga ccaccttgaa tctgagcggc caatggcgta ttcgcaaaga
     7741 tatccgtatg aatctggcca tcaacaacct gacagaccgc aaatactgga actggtccga
     7801 tgtgcagggc ttggcctcca atgcaagtcc ggtggtcgtg gacgcctaca cccagcccgg
     7861 ccgccacgcc aacgtgtctt tggtggtcga tttctaggac ttctcagtcc ggatgggcac
     7921 cggcatcggc gtcgggcaca tccccgatgg ccaggcgcgt gagttctgcc tgcc
//
LOCUS       DAOSJM010000065         7923 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8475_length_7923_cov_4.335791, whole genome shotgun sequence.
ACCESSION   DAOSJM010000065 DAOSJM010000000
VERSION     DAOSJM010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8475_length_7923_cov_4.335791"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="PKX82_03555"
     CDS             <1..255
                     /locus_tag="PKX82_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOF51356.1"
                     /translation="ERMEFIDWIANNALAGDVIPGAGSLRKVRWSRQGIGKRGGARVI
                     YFNRLASGEVVLLLVYAKAKFDNLRPEFLLKLKERLDEKN"
     gene            242..532
                     /locus_tag="PKX82_03560"
     CDS             242..532
                     /locus_tag="PKX82_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOF51357.1"
                     /translation="MKKIKPSADPEMAEFEAALLRSMDQVARGEGRVTTPAQIVARRV
                     GRPAGTVKAAPKVSTTIRLSPDVVQAFRAAGDGWQTRIDAALKDWLRTHSPV"
     gene            559..1026
                     /locus_tag="PKX82_03565"
     CDS             559..1026
                     /locus_tag="PKX82_03565"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF51358.1"
                     /translation="MTSVLPRVQAGSFLTLHYRLSGPAGDIINTFDGKPATLSLGTGE
                     LSPAVEACLIGMEEGARTTFELPAGAAFGERNPDMQQWLARKVLKDMGDPLETYSVGD
                     VVQFPTPDGQGQFAGVVLQLRGETPAEQAVLFDFNHPLAGQPVTFEVQLIGVL"
     gene            1023..1967
                     /gene="ispH"
                     /locus_tag="PKX82_03570"
                     /gene_synonym="lytB"
     CDS             1023..1967
                     /gene="ispH"
                     /locus_tag="PKX82_03570"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOF51359.1"
                     /translation="MSVMPSEIILAEPRGFCAGVDRAIEIVDRALAKFGRPIYVRHEI
                     VHNTYVVNDLKNRGAIFIEELDDVPAGATLVFSAHGVSKAIQREAERRGFQIFDATCP
                     LVTKVHVEVAKLHKEGYEFIMIGHKGHPEVEGTMGQLDGGIHLVEDVADVARIQPAQT
                     AKLAVVTQTTLSVDDASEIAAAVKARFPQVREPKQQDICYATQNRQDAVKVMSGQVDI
                     VIVVGSPTSSNSNRLAELARKLGVTSYMVDNADELRSAWFEGRSRVGLTAGASAPEIL
                     VKAVIDRIRALGAVSVRKMDGIEETVKFPLPKGLKLDV"
     gene            2058..3374
                     /gene="serS"
                     /locus_tag="PKX82_03575"
     CDS             2058..3374
                     /gene="serS"
                     /locus_tag="PKX82_03575"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOF51360.1"
                     /translation="MLDITLLRKDLDSVVNRLQTRKNPQAFLDVAAFTALEAERKTLQ
                     MRTEEMQAKRNTVSKQIGMLKGKGQHAEADAAMAEVAGLKAELDASATRLDAIQTEMQ
                     TLLLAVPNLPHESVPVGADEHGNVVVRSWGTPKAFDFPVKDHVDLGDPLGLDFEMGVK
                     LTGSRFTVMKGQIARMHRALAQFMLDVQTTEHGYTECYTPYIVNGDSLRGTGQLPKFE
                     GDLFAAKKGGQDGEAQPDNTALYLIPTSEVPLTNFVRDVVMPEADLPIKLTAHTPCFR
                     SEAGSAGRDTRGLIRQHQFDKVEMVQIVHPEKSYEALEQMTGHAEAILQKLGLPYRVM
                     SLCTGDMGFGASKTYDLEVWLPAQNTFREISSVSNCEAFQARRLQARYKNAQGKNELV
                     HTLNGSGLAVGRALVAVLENYQNADGSVTVPEVLRPYMGGVAVLKA"
     gene            3437..3524
                     /locus_tag="PKX82_03580"
     tRNA            3437..3524
                     /locus_tag="PKX82_03580"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3471..3473,aa:Ser,seq:cga)
     gene            3598..4092
                     /locus_tag="PKX82_03585"
     CDS             3598..4092
                     /locus_tag="PKX82_03585"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOF51361.1"
                     /translation="MKSYSLLFVCMGNICRSPTAHGVFRHKVRSAGLESRIQVDSAGT
                     HNYHPNSPPDERSQEHASLRGYDLSDLRARQIRDTDFEAHDLILVMDWDNLALVQEIC
                     PAEHQSKVRRLTEFCMTHESPVVPDPYYGGATGFEQVLDLVEDACEGLLQKLQRGEIT
                     ASRG"
     gene            complement(4073..5194)
                     /locus_tag="PKX82_03590"
     CDS             complement(4073..5194)
                     /locus_tag="PKX82_03590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51362.1"
                     /translation="MHKADFLIIGAGIAGASIGYWLAPHGRVVILERESQPGYHSTGR
                     SAALYMASYGSEQVRGLTRASLAFLSHPPEGFADYPLLKPRGALMVATSEQLDALQAH
                     WAMLHALDPHARLISGQEACAMVPVLRPDQVAAAVYEPDAFDMDVDAIHSGFLKGLRR
                     TGGQIVCNAEVTALERHGDAWTVTASGQSWTAPVVINAAGAWAEVVGQMAGAQPIGLV
                     PHRRSAFIFQPPQGAQIDHWPMVISVAEDCYFKPDAGMLLGSPANADPVEPQDIQPEE
                     LDVAMGIHRISEMTSLEIRRPTRTWAGLRSFVDDGGLVGGFDPLASGFFWVAAQGGYG
                     IQTCVAMGEACAALARGQPIPEHIRAQGVTEQLLSPARL"
     gene            complement(5252..5716)
                     /locus_tag="PKX82_03595"
     CDS             complement(5252..5716)
                     /locus_tag="PKX82_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51363.1"
                     /translation="MNPDHHPTSPETGPQGPASAQAQLDLVPELVAKVYEEAPPVVRG
                     RLIEHLLKPLSLLSLVAVANGVFANIALGSGSPVLQVNPEDAQRIGTRDVAALVSHVQ
                     QVSVQVVDGLSQILTSSPVLAGSTATAMLLALLAKQAVQRTPIVGNDFDPIS"
     gene            complement(5807..6097)
                     /locus_tag="PKX82_03600"
     CDS             complement(5807..6097)
                     /locus_tag="PKX82_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase with chaperone activity"
                     /protein_id="HOF51364.1"
                     /translation="MSEDNQIVIPESFLALYLSPGRFKPSAPQAEILGRYELCEDMAQ
                     MLLDTANTKKWELGVETQDIVARIQQGLQGGEAGLSEAEAQWIALRLAELMD"
     gene            complement(6113..6523)
                     /locus_tag="PKX82_03605"
     CDS             complement(6113..6523)
                     /locus_tag="PKX82_03605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF51365.1"
                     /translation="MKSPARLCLAIAATAFCSLSAIAADTQTLPNGVKVTHTVAGSGA
                     SPKATDAVKVHYRGTLADGKEFDSSYKRNAPATFPLNRVIPCWTEGVQKMKVGGKATL
                     VCPPATAYGERGAPGAVPPNATLTFEVELLSIER"
     gene            6609..7214
                     /locus_tag="PKX82_03610"
     CDS             6609..7214
                     /locus_tag="PKX82_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51366.1"
                     /translation="MKTLLTACTLILGLLAGPTVQAQTRLPQRDLTVEVRQVEEGQEN
                     GQEEGASYRAGPQSAALLTAQKVQVRNGEKATLRMHQSVPMQWVQSVQAPSKQSGAGV
                     SQSLHWFDAGQTLVVLPRWPGGRREVVVELDVRQASLQDGTNANLPSQGQSQWVSTVT
                     VPLDRWVTVAASGNPAPPKGSYSSEAASSPRRLLQVRVQLP"
     gene            complement(7225..7857)
                     /locus_tag="PKX82_03615"
     CDS             complement(7225..7857)
                     /locus_tag="PKX82_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding beta-barrel domain-containing
                     protein"
                     /protein_id="HOF51367.1"
                     /translation="MSDFPKDIYTEPSPLDVNTLANLGPLRRMAGIWEGMRGLDVKPK
                     AEGPKKQAFHERMELQPIDPQTNGPQLLYGLRYHTHVTKPDQIKTYHDQVGYWLWEPA
                     TGAIIHTLTIPRGQIAMASGQAAADATEFELVATQGLETFGICSAPFLQHAFKTTEFR
                     IKVTINADGTWTYDEDTVLQILGRDEPFHHTDRNTLHKVAEPTPNPLARG"
BASE COUNT         1533 a         2369 c         2556 g         1465 t
ORIGIN      
        1 gaacgtatgg aattcattga ctggattgcc aacaacgcac ttgcgggtga cgtcattcca
       61 ggcgctggca gcttgcgcaa agtgcgctgg tcgcgtcagg gcatcggaaa acggggcggt
      121 gcacgggtga tctatttcaa tcggcttgca agcggggaag ttgttttgct cctggtctat
      181 gccaaggcca agtttgacaa cttgcgtccc gaatttttat tgaagttgaa ggagcgtttg
      241 gatgaaaaaa attaagcctt ccgcagaccc tgaaatggca gagttcgagg cggccctttt
      301 gcgttctatg gaccaggtcg cccgaggaga agggcgtgtg acaacgcctg cacaaatcgt
      361 cgcgcgtcgc gtcggtcgcc ctgcgggtac tgtcaaagcg gcacctaaag tgtccaccac
      421 aatccgtttg tcacctgatg tggtgcaagc cttcagggct gctggtgatg gatggcagac
      481 acgcattgat gccgcactga aagattggct gcgcacacat tctcccgtct gattcaagac
      541 ctgtttaccg gaatttttat gacctcagtg cttccccgcg ttcaggcggg ctcgtttctc
      601 accctgcatt accgcctgag cggccctgcg ggcgacatca tcaacacctt cgatggcaag
      661 cctgccacgc tgagtctggg tacgggcgaa ctctcgcccg ctgtggaggc ctgcctgatc
      721 ggcatggaag agggcgcacg caccacgttt gagctgcctg cgggcgccgc ctttggtgag
      781 cgcaaccccg acatgcagca atggctggcg cgcaaggtgc tcaaggacat gggcgacccg
      841 ctggaaacct acagcgtggg cgacgtggtg cagttcccca ccccggacgg ccagggccag
      901 tttgccggcg tggtgctgca gctgcgtggc gaaaccccgg cagagcaggc agtgctgttc
      961 gatttcaacc accctttggc tggccagccg gtgacctttg aagttcagct gattggcgtg
     1021 ctatgagtgt catgcccagc gaaatcattc tggcggagcc acgcggtttt tgcgccgggg
     1081 tggatcgcgc gatcgaaatc gtggaccggg cgctggccaa gtttggccgc cccatctatg
     1141 tgcgccatga aatcgtgcac aacacttatg tggtcaacga cttgaagaat cgcggtgcca
     1201 tcttcatcga agagctcgac gatgtgcctg cgggtgcaac tttggtgttc tcggcccacg
     1261 gcgtgagcaa agccatccag cgggaggcag agcggcgcgg tttccagatt ttcgatgcca
     1321 cctgcccgct ggtaaccaag gtgcacgtgg aagtcgccaa gctgcacaag gaaggttatg
     1381 agttcatcat gatcggccac aagggccatc ccgaggtgga aggcaccatg gggcagctcg
     1441 atggcggcat ccatctggtg gaagacgtgg ccgatgtggc gcgcatccag cccgcccaga
     1501 cggccaagct ggcggtggta acccagacga cactgagcgt ggacgacgcg tccgagattg
     1561 cagccgctgt caaggcccgg ttcccgcagg tgcgtgagcc caagcagcag gacatctgct
     1621 acgccaccca gaaccgccag gatgcggtga aggtgatgag cggccaggtc gacatcgtga
     1681 tcgtggtggg cagccccacc agctccaaca gcaaccgctt ggccgaactg gcgcgcaaac
     1741 tgggcgtgac cagctacatg gtggacaacg ccgatgagct gcgaagcgcg tggtttgaag
     1801 gccgcagccg cgtgggtctg acggcgggtg cctcggcgcc cgaaattctg gtgaaggcgg
     1861 tgatcgaccg catccgcgcg ctgggtgccg tgtcggtgcg caagatggac ggcattgagg
     1921 agaccgtgaa gtttcccctg cccaagggcc tgaaactgga cgtctgaccc tcctgataag
     1981 tgctattaaa tatatagctg cttacgcaat aaaatcgggg gccagagccc aatttgactt
     2041 agaagtacga gaacaccatg ctagacatca ccctgctgcg caaagacctg gattcggtcg
     2101 tcaatcgcct gcaaacccgc aagaaccccc aggcgtttct ggatgtggcc gccttcacgg
     2161 ccctggaagc cgagcgcaag accttgcaga tgcgcaccga agaaatgcag gccaagcgca
     2221 acacggtcag caagcagatc ggcatgctca agggtaaagg ccagcacgcc gaggcggacg
     2281 cggccatggc cgaagtggcg ggactgaaag ccgagttgga cgcctctgcc acgcgcctgg
     2341 atgcgattca gaccgagatg caaaccctgc tgctggccgt gcccaatctg ccgcacgaga
     2401 gcgtgccggt gggcgcggac gagcatggca atgtggtggt gcgcagctgg ggtaccccca
     2461 aggccttcga ctttccggtc aaggaccatg tggacctggg tgacccgctg gggctggact
     2521 tcgagatggg cgtcaagctc accggttcgc gttttaccgt catgaaaggg caaattgccc
     2581 gcatgcaccg tgcgctcgcc cagttcatgc tggacgtgca aaccaccgag cacgggtata
     2641 ccgagtgcta cacgccctac atcgtcaacg gtgacagcct gcgtggcacc ggccagttgc
     2701 ccaagtttga aggcgatctg tttgccgcca aaaagggtgg acaggatggc gaagcgcagc
     2761 ccgacaacac cgcgctctac ctgatcccca ccagcgaagt gccgctgacc aattttgtac
     2821 gtgacgtggt catgcccgag gccgacttgc ccatcaaact cacggcccac accccgtgct
     2881 tccgctcgga agcgggcagc gccgggcgcg acacgcgcgg cctgatccgc cagcaccagt
     2941 tcgacaaggt ggaaatggtg cagatcgtgc accccgaaaa gagctacgaa gcccttgagc
     3001 aaatgaccgg ccacgccgaa gccatcctgc agaagctggg ccttccctac cgcgtgatga
     3061 gcctgtgcac cggcgacatg ggctttggcg ccagcaagac ctatgacctg gaagtgtggc
     3121 tgcctgcgca gaatactttc cgcgaaatca gctcggtgtc caactgcgaa gccttccagg
     3181 cccgccgtct gcaagcccgc tacaagaatg cccaaggcaa gaacgaactg gtgcataccc
     3241 tcaacggctc gggtctggcc gtaggccgcg ccctggtggc ggtgctggag aactaccaga
     3301 atgccgacgg cagcgtgacc gtgccggaag tgctgcgccc ctacatgggc ggcgtggcgg
     3361 ttctcaaggc ctgagcaaga gggcaggccg cacagcctgc tagaatcgcg gcttcgtttc
     3421 gaccgacacg attccaggag agatggcaga gtggtcgaat gtacctgact cgaaatcagg
     3481 cgtaccgcaa ggtaccgtgg gttcgaatcc cactctctcc gccaaatggc aagggcctct
     3541 gcagtgcaga ggccctttct actggtgagg ccggaacctg caggaggctc ctacgcaatg
     3601 aagtcctact ccctactctt tgtctgcatg ggcaacatct gtcgcagtcc tactgctcat
     3661 ggcgtgttcc ggcacaaggt gcgttcagcc ggtctggagt cgcgcataca ggttgactcg
     3721 gcgggaaccc acaactacca ccccaacagc ccgccggatg agcgctcaca ggagcacgcc
     3781 agcctgcgtg ggtacgacct gtccgacctg cgggcgcgcc agatccggga tactgacttt
     3841 gaggcgcatg atttgattct ggtgatggac tgggacaatc tggccctggt gcaggagata
     3901 tgccccgcgg aacaccagag caaagtcagg cgcctgaccg agttctgcat gacccatgaa
     3961 agcccggtgg tgccagaccc ttactatggc ggcgcgacag gttttgagca ggtgctcgat
     4021 ctggtcgagg atgcctgcga ggggctgctg caaaagctcc agcgcggaga aatcacagcc
     4081 tcgcggggct gagcagctgt tccgtaacgc cctgtgcgcg gatgtgctcc ggaatgggct
     4141 ggccccgtgc cagtgcggcg caggcttcgc ccatggccac acaggtctgg atgccatagc
     4201 cgccctgcgc agccacccag aagaacccag atgccagcgg atcaaatcca cccaccagac
     4261 caccatcgtc cacaaacgag cgcagcccgg cccaggtgcg tgtggggcgg cggatttcca
     4321 gcgaggtcat ctccgaaatg cggtggatgc ccatggcaac gtcgagttcc tcgggctgga
     4381 tgtcctgcgg ctccaccggg tctgcgttgg cgggggagcc cagcagcatg ccggcgtccg
     4441 gtttgaaata gcagtcttcc gccacgctga taaccatcgg ccagtggtcg atctgggcac
     4501 cctgcggtgg ctggaaaatg aaagccgagc ggcggtgcgg caccagacca atgggttgtg
     4561 cgcccgccat ctggcctacc acctcggccc aggcgcctgc ggcattgatc accactgggg
     4621 ctgtccagga ttggccactt gcggtaacgg tccatgcatc gccgtggcgc tccagtgcgg
     4681 tcacttcggc attgcagaca atctggccgc cggtacggcg aaggcctttg aggaaaccgg
     4741 aatgaatagc gtccacatcc atatcaaacg catcgggctc gtagaccgca gccgcgacct
     4801 gatccgggcg cagcaccggc accatggcgc aggcttcctg tccgctgatc aaccgggcgt
     4861 gggggtccag cgcgtgcagc atggcccagt gcgcctgcag tgcgtccagt tgctctgaag
     4921 tcgccaccat cagggccccg cggggcttga gcagtgggta gtccgcaaat ccttcaggcg
     4981 gatgggacag aaacgccagg ctggcgcggg tcaggccgcg cacctgttcc gatccgtaac
     5041 tggccatgta cagggcggcg gagcggcctg tggagtggta gccgggctgc gattcacgct
     5101 ccagaatgac gacacggccg tgcggcgcca gccagtagcc gatggacgca ccggcaatgc
     5161 cggcgccgat gatgaggaag tcggctttgt ggatgtttgt gctccggttt ggtgattccc
     5221 gttggacttt accaagcccg gcgcaaggct gctatgaaat cgggtcgaag tcgttgccta
     5281 cgatgggtgt gcgctgcacc gcctgcttgg ccagcagggc gagcagcatg gccgtcgcag
     5341 tggaacccgc caggacgggt gaagaagtca ggatctggga gagaccatcg accacctgga
     5401 cgctgacctg ctgcacatgg ctgaccaggg cggccacgtc acgtgtgcca atgcgctgcg
     5461 catcttccgg gttgacctgc agaactggcg agccactgcc cagcgcgatg ttggcaaaca
     5521 cgccgttggc taccgccacc agcgacagca ggctcagggg tttcagcagg tgttcaatca
     5581 ggcgaccccg cactaccggt ggggcttctt cgtacacctt ggcaaccagt tcgggcacca
     5641 ggtccagttg cgcctgcgct gacgccggcc cctgagggcc ggtttcggga gacgtagggt
     5701 ggtgatcagg gttcatggtc atcatggtga catcgaaatg tgaccctgca atgaacatga
     5761 agtaaaagaa tgtaacgggc cgctgaccct tgatccttct gctggctcag tccatcaact
     5821 ccgcgagtcg caacgcgatc cattgcgctt cggcctcgct caggcccgct tctccgccct
     5881 gcagaccttg ctggatgcgc gccacgatgt cctgcgtttc cacgcccaat tcccatttct
     5941 tggtgttggc ggtgtcgagc agcatctgcg ccatgtcctc gcacaactcg tagcgcccca
     6001 gaatctcggc ctgcggggcg ctcggcttga accgcccggg gctcaggtac agggccagaa
     6061 acgactccgg gatgacgatc tggttgtctt ccgacatggc gttgcgggcg gatcagcgct
     6121 cgatggacag cagttccact tcaaaggtca gggtggcatt gggcggcacg gcgccggggg
     6181 caccgcgctc gccgtaggcc gtagcaggcg ggcagaccag cgtggccttt ccaccaactt
     6241 tcatcttctg cacgccttcg gtccagcagg gaatcacgcg attcagcgga aaggtggctg
     6301 gcgcattgcg tttgtaggag ctatcaaatt ccttgccatc tgccagcgtg ccgcgatagt
     6361 gcaccttgac ggcatcggtg gctttcgggg atgcgccgct gcccgccacg gtgtgggtga
     6421 ccttgacgcc gttgggcagg gtttgggtgt cggcagcaat agccgacagg ctgcaaaaag
     6481 cggtggcggc gatagcaagg cacaggcggg caggtgattt catggtggtt tgtcagggtt
     6541 gaaaacagtg tgagctgccg agtgtacggc tacctgcaaa atcccaatcg tggaacaatg
     6601 ctttgaccat gaaaacactg ctgactgcct gcaccctgat ccttggcctt ctggcaggcc
     6661 ccacggtgca agctcagacc aggttgcccc agcgcgacct gaccgtggaa gtgcgccagg
     6721 tggaagaagg gcaggagaat gggcaggagg aaggagccag ctaccgggcc ggcccgcagt
     6781 ctgccgcctt gctcacggcg cagaaagtgc aggtgcgcaa tggcgagaag gccacgctgc
     6841 gcatgcacca gtctgtgccc atgcagtggg tgcaatcggt gcaggcacct tccaaacaaa
     6901 gcggtgcagg tgtgagccag tcgctgcact ggttcgatgc cggccagacc ctggtggtgc
     6961 taccgcgttg gcccggtggc aggcgcgaag tggtggtgga gctggatgta cgccaggcca
     7021 gtctgcagga cggtaccaat gccaatcttc cgtcgcaggg gcagtcgcag tgggtgagca
     7081 cggtgaccgt gccactggac cgctgggtca cggtggccgc atcgggcaac cccgcgccac
     7141 caaagggcag ctacagcagc gaggcggcca gcagccccag gcgcctgctg caggtgcgcg
     7201 tacagcttcc ctgaagctgg gttctcagcc gcgcgccagc gggttgggcg tgggttccgc
     7261 caccttgtgc agggtgttgc ggtcggtgtg gtggaacggt tcgtcgcgtc ccaggatctg
     7321 cagcacggtg tcttcgtcat acgtccaggt gccatcggcg ttgatcgtca ccttgatgcg
     7381 gaactcggtg gtcttgaagg cgtgctgcag gaatggcgcg gagcaaatgc caaaggtttc
     7441 cagcccctgg gtggccacca gttcaaattc ggtggcatcg gccgccgcct gacccgaggc
     7501 catggcaatc tgcccgcgcg gaatcgtcag cgtgtggatg atggctccgg tagcgggctc
     7561 ccacagccag tagcccacct ggtcgtggta ggtcttgatc tggtcgggct tggtgacgtg
     7621 ggtgtggtag cgcaggccgt acagcagttg tgggccattg gtttgcgggt cgatgggctg
     7681 caattccatg cgctcatgga aggcctgctt cttcggaccc tcggccttgg gcttcacgtc
     7741 cagaccccgc atgccttccc aaatacccgc catgcggcgc agcgggccca ggttggccag
     7801 ggtgttcacg tccagcggcg aaggctcggt gtagatgtct ttggggaagt cactcatgtg
     7861 ctggctccag tgaaattcat gaataaaaaa tgcctctagc ccccgtgaaa catgcgggag
     7921 tag
//
LOCUS       DAOSJM010000066         7902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8505_length_7902_cov_3.935644, whole genome shotgun sequence.
ACCESSION   DAOSJM010000066 DAOSJM010000000
VERSION     DAOSJM010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8505_length_7902_cov_3.935644"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PKX82_03620"
     CDS             <1..608
                     /locus_tag="PKX82_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF51368.1"
                     /translation="LSLLIGIAVVALSVILGTVLGLLAGYFRGVFEITIMRLMDIILT
                     LPSLLLAIVIVAILGPGMMNAMLAVSIVVLPHYVRITRAAVISETSRDYVTAARMGGA
                     GHIRLMFSEVLPNCTAPLIVQASLGISTAILDAAALGFLGLGAQPPSPEWGTMLADAR
                     EFVLRAWWVVTFPGLAILITVLAFNLLGDGLRDALDPKLKR"
     gene            616..1611
                     /locus_tag="PKX82_03625"
     CDS             616..1611
                     /locus_tag="PKX82_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF51369.1"
                     /translation="MALLEIENLSVEFPSHNATMHAVDGVSLSIEAGEVLGIVGESGS
                     GKSVTMMALMGLVSFPGRVKADVLRFDGHDLLALSDHARRKLIGKDLSMIFQDPTTSL
                     NPCFTVGFQLAETLRLHMGMDKAAARRRSIELLEQVGIPAPESRLSVYPHQLSGGMSQ
                     RVMIAMAIACNPKLLIADEPTTALDVTIQAQILDLLRSLQKDRGMALVLITHNMGVVS
                     EMAQRVAVMYAGQLMEQRDAQALFHSPQHPYTEALMASMPERSQGSQRLTTIPGMVPG
                     LYDRPSGCLFGPRCSHAKTMECAQRPALQATTDGAVRCHFPLGRATHDQVHEVKV"
     gene            1608..2618
                     /locus_tag="PKX82_03630"
     CDS             1608..2618
                     /locus_tag="PKX82_03630"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HOF51370.1"
                     /translation="MTVQTTSTPNANTTVVQARDLRKVYPISNGFLRKADQLQAVGGV
                     SFSVRAGQTLAIVGESGCGKSTLARMVSLIEMPSAGALTVDGVDVVAASDHDKQLLRQ
                     KVQLVFQNPYGSLNPRKRIGQILEAPLEINTRLNATQRAEKARAMLALVGLRPEHYDR
                     YPHMFSGGQRQRIAIARALMLNPALVVADEPVSALDVSIQAQVLNLLADLQRDLGLAY
                     LFISHDLGVVRHIAHDVLVMYLGHAVEQGEKETIFSNPLHPYTQALLASTPGLAGSGP
                     ARHRMVLKGELPSPLNPPKGCVFSTRCPHVTERCQAERPPLQELMGRSVACFEAERIA
                     TR"
     gene            2682..4301
                     /locus_tag="PKX82_03635"
     CDS             2682..4301
                     /locus_tag="PKX82_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF51371.1"
                     /translation="MTRSTARMRLSKSALLCAVALPAVLAMASASAKTLVYCSEGSPE
                     NFYPGINTTGTSFDANEQIYDNLVDFERGGTTVIPNLATKWTISKDGTEYTFSLRKGV
                     KWHSNKNFKPSRDFNADDVLFMFERQWKENDPYYKVTSSNHAYFGDMGMPKLLKSVEK
                     IDEYTVKVTLNKPEAPFLANLAMQFAGIQSKEYAIAMLKAGTPEKIDQDPLGTGPFQL
                     VQYQKDAIIRYKAFPQYWEGKAKIDDLIFSITPDASVRWAKLQKGECHVMPYPNPADL
                     DAMRKDANVTVLEQPGLNIGYLAYNTTKKPFDDVRVHKAMNMAIDKKAIVSAVYLSTG
                     VPATNPIPPGQWSYNKAIKDDAFNPTEAKKLLAAAGYPNGFTTDLWAMPVQRPYNPNA
                     KRIAELMQADLAKVGVKAEIKSFEWGEYRKRMQAGEHQMGMLGWTGDNGDPDNFLHTL
                     LGCDSAKGGGSNVAKFCHKPFDDLVVKAKTVTNPAERTKLYEKAQVIFKEQAPWFTIA
                     HAVQLKPVRKEVIDFKLSPFGRHTFYGVDIK"
     gene            complement(4390..5364)
                     /locus_tag="PKX82_03640"
     CDS             complement(4390..5364)
                     /locus_tag="PKX82_03640"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOF51372.1"
                     /translation="MKKRAGHAYWTGAKALFCAWMLLCAPVQARAQVHRADASGSATI
                     TAPGSYAYSMVYGGHKRLYRVHVPASYVPGKPVPLVFALHGGGGNMDYQANDTYYGQI
                     AQAEKDGYVVVFPNGFSRLPGGKLATWNAGNCCGAARDKGIDDVGFVREIVRRLKTTL
                     SVDARRIYANGMSNGGMMAYRLACDAPDVFRAVASVAGTDNTASCAPAKAVSVLHIHA
                     RDDEMVLFNGGAGRDRSAVTDFVAVPDSVAKWVRLNACSVTPKRVLEKSGAYCDSYSP
                     CRDGAEVKLCVTETGGHSWPGGAKVRGGTAGSSALSATGEIAAFFGSH"
     gene            complement(5385..7145)
                     /locus_tag="PKX82_03645"
     CDS             complement(5385..7145)
                     /locus_tag="PKX82_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOF51373.1"
                     /translation="MSNTTSPSLAPTLAGHLLVQCLVEQGATHAFGVPGESYLAVLDG
                     FHLYRDQIQFVINRQEGGAAFMAEAHGKLTGRPGVCFVTRGPGATNASIGVHTAFQDS
                     TPMVLFVGDVASDQRDREAFQEMDYRVMFGPSALGMAKRVERIDEVACIPEYVARAFA
                     TAMNGRPGPVVLVLPEDMLTQTLVADKATGQLPQPLPRVEPVEAWSDPGSLRRLRELL
                     LKAERPFVIAGGGGWTPQAAQALQRFAENWRLPVGNAFRFQDTFDNHHPLYAGDVGIG
                     INPLLAKRIKESDLIIAIGPRLGEMTTGGYTLLQAPKAAQTLVHIHASAEELNRVYQA
                     DLAIHASMRAAARSLEVLTAPVSVPWETWTQGCNADYLANLQPSVIQDLPADTPAGLV
                     NMPQVIATLQKHLPADAVLTNGAGNFASWLHRFYRYTGLAAGHKTQLAPTNGAMGYGV
                     PAGIAAAITTNRVAFTIAGDGDFLMNGQELATAVQHGAKTIILVLNNGMYGTIRMHQE
                     REYPQHVAGTKLKNPDFVALAKAYGYAAVRIQRSDAFEAELLAALARPEGTLIEVVLE
                     PELISTRGTLSAITRTALAR"
     gene            complement(7176..>7902)
                     /gene="phhA"
                     /locus_tag="PKX82_03650"
     CDS             complement(7176..>7902)
                     /gene="phhA"
                     /locus_tag="PKX82_03650"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HOF51374.1"
                     /translation="RLLPGLACDAFIEGMRRLPMAADRIPDFEALSEVLHRATGWQVV
                     AVPGLVPDDVFFEHLANRRFPAGNFIRGAHELDYLEEPDVFHDVFGHVPMLMHPVIAD
                     FIQAYGQGGLRAQKRGKLAELARVYWYTVEFGLVRQGGGTRIYGAGIASSFSESTFAV
                     QSASPNRIGFELERVMRTHYRIDDFQESYFVLDSLDDLLELAHIDFEPYYTALDSGPT
                     FTPGQVLAQDQVLHAGTGEYHAN"
BASE COUNT         1585 a         2548 c         2279 g         1490 t
ORIGIN      
        1 gcctgtcctt gttgatcggc attgcggtcg tagctctgtc cgtgattctg ggcaccgtgc
       61 tgggcctgct ggcaggctac ttccgtgggg tcttcgagat caccatcatg cgcctgatgg
      121 acatcatcct gaccctgccc agccttttgc tggccattgt gattgtggcc attctcgggc
      181 ccggcatgat gaatgccatg ctggcggtat ccattgtggt gctgccccac tatgtgcgca
      241 ttacccgcgc agcggtcatc tccgagacat cgcgcgatta cgtgaccgcc gcccgcatgg
      301 gtggtgccgg ccacatccgt ctgatgttca gcgaagtgct gcccaactgc actgcgccgc
      361 tgatcgtgca agcctcgctg ggcatttcca ccgccattct ggatgcggca gcgctgggtt
      421 ttctgggtct gggtgcgcag ccaccctctc cggaatgggg aaccatgctg gccgatgcgc
      481 gcgaatttgt gctgcgcgcc tggtgggtcg tgaccttccc gggcctggcc atcctgatca
      541 ctgtattggc atttaacctg ctgggagacg gcttgcgcga cgcgctggac cccaagctca
      601 agcgataaga acaccatggc actgcttgaa atcgaaaacc tgtcggtgga gtttccatcg
      661 cacaacgcca ccatgcacgc cgtcgatggt gtgagcctgt ccatcgaagc cggagaagtg
      721 ctgggtattg tgggtgaatc cggctccgga aaaagcgtca ccatgatggc cctgatgggt
      781 ctggtgtcct ttcccggtcg cgtcaaggcc gatgtgctgc gctttgatgg ccacgatctg
      841 ctggccctgt cggaccatgc acggcgcaag ctcatcggca aagacctgtc catgattttt
      901 caggatccca ccaccagtct gaacccgtgc tttaccgtgg gcttccagct ggcagagacc
      961 ttgcggctgc acatgggcat ggacaaggct gcggcgcgcc ggcgcagcat cgagctgctt
     1021 gagcaggtgg gcattcccgc gccagaaagc cgcctgtcgg tgtacccgca tcagctatcg
     1081 ggcggcatga gccagcgcgt gatgattgcc atggccattg cctgcaatcc caaactgctg
     1141 atcgccgatg aaccgaccac cgcgctggat gtcaccatcc aggcacagat tctggacctg
     1201 ctgcgcagcc tgcaaaaaga ccgcggcatg gcgctggtgc tcatcaccca caacatgggt
     1261 gtggtcagcg aaatggcgca acgcgtggcc gtcatgtatg ccggacagtt gatggaacag
     1321 cgggatgccc aggcgctgtt tcacagcccc cagcacccct acactgaagc gttgatggcc
     1381 tccatgccag aacgcagcca aggcagccaa cgcctgacga ctatcccagg catggtcccg
     1441 ggcctgtatg accgacccag tggctgcctg ttcggaccac gttgcagcca tgcaaaaacc
     1501 atggagtgcg cacagcgacc tgcgttgcag gcaacaaccg atggcgccgt gcgttgccat
     1561 ttcccactgg gaagagcaac acacgaccag gtccatgagg tcaaggtatg acagtacaaa
     1621 ccacttccac tccgaacgcc aacaccaccg tggtgcaggc gcgcgacctg cgcaaggtct
     1681 accccatcag caatggtttt ctgcgcaagg cagaccagtt gcaggccgtc ggtggtgtgt
     1741 cgttctcggt acgtgccggt cagacactgg ccatcgtggg cgaatcgggt tgcggcaagt
     1801 ccacactggc ccgcatggtc tcgctcatcg aaatgccatc ggctggcgcg ttgaccgtgg
     1861 acggcgtcga cgtggtggcc gcctccgacc acgacaaaca gttgcttcga caaaaagtgc
     1921 agctggtctt ccagaacccc tacggctcgt tgaatccgcg gaaacgtatc ggccagattc
     1981 tggaggcgcc gctggaaatc aacacccggc tcaacgcaac gcaacgcgcg gaaaaagccc
     2041 gcgccatgct ggctttggtc gggctgcggc cagagcacta tgaccgctat ccacacatgt
     2101 tttcgggcgg gcagcgccag cgtattgcga ttgcgcgcgc gctcatgctc aaccctgcac
     2161 tggtggtggc cgatgagccg gtttccgcgc tcgatgtctc cattcaggcc caggtgctga
     2221 acctgctggc cgacctgcag cgtgacttgg gattggccta cctgttcatt tcgcacgatc
     2281 tgggggtggt gcggcacatt gcccatgacg tgctggtgat gtacctgggc catgccgtcg
     2341 agcagggtga gaaagaaacc attttcagca accctctgca tccctacacc caggccttgc
     2401 tggcgtccac gccgggcctg gcgggcagtg gtccggcaag gcaccgcatg gtgctcaagg
     2461 gagagttgcc gtcgccactg aatccaccga agggatgcgt gttttcgacc cgctgtccgc
     2521 atgtgactga acggtgccag gcagagcgcc ccccactgca ggaactgatg gggcgctcgg
     2581 tcgcgtgctt tgaagcagaa cgcatcgcaa cccggtaaca accccagtag gttgtgcctg
     2641 ttagttggcg taacatgact tttttttgaa ggagctaatc aatgacccgt tctaccgccc
     2701 gcatgcgctt gagcaaatct gctctgttgt gcgcagtggc cctgcccgcc gttctggcca
     2761 tggcctcggc ctcggccaaa accttggtgt actgctccga aggcagcccc gagaatttct
     2821 atcctggtat caacaccaca ggcacctcgt ttgatgccaa cgaacagatt tatgacaacc
     2881 tggtggactt tgagcgcggc ggcaccacgg tgatccccaa cctggccacc aaatggacca
     2941 tctccaaaga tggaaccgag tacacctttt ccctgcgcaa gggtgtgaaa tggcactcca
     3001 acaaaaactt caagccgtca cgcgacttca atgccgatga cgtgctgttc atgttcgagc
     3061 gccagtggaa agagaacgac ccgtattaca aggtcaccag ttccaaccat gcttacttcg
     3121 gcgacatggg catgcccaag ctgcttaaat cggtagaaaa aatcgatgag tacaccgtca
     3181 aagtcacctt gaacaagccc gaggccccct tcctggcgaa tctggcaatg cagttcgcag
     3241 gcattcagtc caaggaatac gccattgcca tgctgaaagc tggcacaccg gaaaaaatcg
     3301 accaagaccc cctgggcacg ggcccattcc agctggtgca ataccagaag gacgccatca
     3361 tccgttacaa ggcctttccc cagtactggg aaggcaaagc caagattgac gacctgatct
     3421 tctccatcac tcccgacgct tccgtgcgct gggccaagct gcaaaagggt gagtgccatg
     3481 tcatgcccta ccccaacccg gctgatctgg acgccatgcg caaggacgcc aacgtcaccg
     3541 tactggaaca acctggtctg aacattggct acctggccta caacaccacc aagaaacctt
     3601 ttgacgatgt gcgcgtgcac aaagccatga acatggccat cgacaagaaa gccatcgtct
     3661 cggcggtgta tctgagcacg ggcgtgcctg ccaccaaccc catccctcca ggacagtggt
     3721 cgtacaacaa ggccatcaag gatgacgcct tcaaccccac agaagccaaa aagctgctgg
     3781 cagcagccgg ctaccctaac ggcttcacca cggacctgtg ggccatgccc gtgcagcggc
     3841 cttacaaccc caatgccaag cgcattgccg agttgatgca ggctgacctg gccaaggtcg
     3901 gcgtcaaggc cgaaatcaag agctttgaat ggggcgagta ccgcaagcgc atgcaggcag
     3961 gtgaacacca gatgggcatg ctgggctgga ccggtgacaa cggtgaccca gacaacttcc
     4021 tgcacaccct gctgggctgc gactctgcca agggcggtgg ctccaacgta gcgaagttct
     4081 gccacaagcc tttcgacgat ctggtcgtca aagccaagac cgtgaccaat ccggctgagc
     4141 gcaccaagct gtacgaaaaa gcccaggtca tcttcaagga gcaggctccc tggtttacca
     4201 ttgcccacgc ggtgcagttg aagcccgtgc gcaaggaagt catcgacttc aaactgagcc
     4261 cgtttgggcg ccacactttc tacggtgtgg atatcaagta atcagcgtct gctgaaacag
     4321 gaagggccgg taattaccgg cccttttttt ctgcagcttc cagagcgaat ccccaaccaa
     4381 catccagctt caatggctgc caaaaaaagc ggctatttct cccgtcgccg acagggctga
     4441 agaccccgcc gtgccaccgc gtaccttggc accacccggc caggaatggc ccccggtctc
     4501 cgtcacgcac aacttcactt ctgcaccgtc cctgcacggg ctgtagctgt cgcaataggc
     4561 accactcttt tccagcaccc gcttcggcgt gacactgcaa gcattgaggc gcacccactt
     4621 ggccaccgaa tcgggcactg ccacaaagtc ggttaccgca gagcggtcgc gccctgcccc
     4681 gccgttgaac agcaccatct catcgtcccg cgcatggatg tgcagcaccg acaccgcttt
     4741 ggcaggagca cacgatgcag tgttgtccgt acccgccacc gaagccaccg cccggaatac
     4801 atcgggcgca tcgcaggcca ggcggtaggc catcatgccg ccgttggaca tgccgttggc
     4861 gtagatgcgg cgggcatcca cgctgagcgt ggtcttgagg cggcgcacga tctcgcgcac
     4921 aaagcccaca tcgtcaatgc ccttgtcccg tgcagcaccg cagcagttgc ccgcattcca
     4981 ggtggccagc ttgccgccag gcaagcgact gaacccattg gggaacacca ccacatagcc
     5041 gtccttctct gcctgcgcaa tctgcccgta gtaggtatcg ttggcctggt agtccatatt
     5101 cccgccaccg ccgtgcaagg caaacaccag cggcactggc ttgccgggca catagctcgc
     5161 cggcacatgc acacggtaca gccgcttgtg cccaccgtag accatggagt aggcatagct
     5221 tccgggcgcg gtgatggtgg cggatccgga cgcatctgcc ctatgaacct gcgcccgtgc
     5281 ctgcactggc gcgcacagca gcatccatgc acaaaacagg gccttagccc ccgtccaata
     5341 tgcgtgacca gctctctttt tcatagcaaa tccactggta aaacctagcg cgccagcgca
     5401 gtccgggtaa tcgccgacag cgtgccgcgg gtactgatga gttcgggctc cagcaccacc
     5461 tcgatcagcg tgccctccgg ccgcgccagc gctgccagca gctcggcttc aaacgcatcg
     5521 ctgcgctgga tgcgcacagc cgcatagcca taggccttgg ccagcgccac aaagtcgggg
     5581 ttctttagct tagtgcccgc cacatgctgc gggtactcgc gctcctggtg catgcggatg
     5641 gtgccgtaca tgccgttgtt gagcaccagg atgatggtct tggcaccgtg ctgtaccgcc
     5701 gtggccagct cctgcccgtt catcaggaag tcgccatcgc ccgcgatggt gaacgcaacc
     5761 ctgttggtgg tgatggccgc agcgataccc gcgggtacgc catagcccat ggcaccattg
     5821 gtgggtgcca gttgcgtctt gtggcccgca gccaggccgg tgtagcggta gaaacggtgc
     5881 agccagctgg cgaagttgcc tgcgccattg gtcaacaccg cgtctgctgg cagatgtttt
     5941 tgcagtgtgg caatcacctg cggcatattg accagtcccg ccggcgtgtc cgcgggcaag
     6001 tcctggatga cggacggttg caaattggcc aggtagtcgg cattgcagcc ttgggtccac
     6061 gtttcccagg gtacgctcac cggtgcagtc agcacttcca gtgaacgggc agcggcgcgc
     6121 atgctggcgt ggatggccag atccgcctgg tacacccggt tcagctcctc cgcactggcg
     6181 tggatgtgca ccagcgtctg cgctgctttt ggtgcttgca acagcgtgta gccgccggtg
     6241 gtcatctcgc ccagccgtgg cccgatggcg atgatcaggt cgctctcctt gatgcgcttg
     6301 gccagcaacg gattgatgcc aatgcccaca tcgcccgcgt acagcgggtg gtggttgtca
     6361 aaggtgtcct gaaaccggaa cgcgttgccc accggcaacc gccagttctc ggcaaagcgc
     6421 tgcagcgcct gcgcagcctg cggggtccag ccaccgccac cggcaatgac aaagggtcgc
     6481 tctgctttca atagcaactc acgcagtctg cgcaaggagc cggggtcact ccaggcctcc
     6541 accggctcca cgcgcggcag cggctggggc agttggcctg tggccttgtc cgccaccaga
     6601 gtttgcgtga gcatgtcctc cggcaacacc agtaccaccg gaccgggccg gccattcatc
     6661 gcggtggcaa aggcgcgcgc cacgtactcc ggaatgcacg ccacctcatc gatgcgctcc
     6721 acccgcttgg ccatgcccag tgcgctgggg ccaaacatca cgcggtagtc catctcctga
     6781 aatgcttcgc ggtcgcgctg gtcgctggcc acatcgccga caaacaacac catcggtgtg
     6841 gaatcctgaa acgcggtgtg tacgccaatc gacgcgttgg tggcacccgg cccccgggtc
     6901 acaaagcaca cacccggcct gccagtcaat ttgccatgcg cttcggccat gaaggcggca
     6961 ccgccctcct ggcggttgat cacaaactgg atctggtcgc ggtacagatg gaagccatcc
     7021 agcaccgcca gatagctttc gccgggtacg ccaaacgcgt gggttgcgcc ctgctcgaca
     7081 aggcattgca ccagcaggtg gccggccaga gtgggagcta atgagggaga agtggtgtta
     7141 ctcatgcaca ccattgtgcc gactaaatcc ggtggctagt tcgcgtggta ttcgcccgtg
     7201 cctgcgtgca gcacctgatc ctgcgccagc acctgccccg gagtgaaagt ggggccgcta
     7261 tccagcgccg tgtagtaggg ctcaaagtca atgtgcgcca gctccagcag gtcatccaga
     7321 ctgtcgagca caaagtagct ttcctgaaaa tcgtcaatgc ggtagtgggt gcgcatcacg
     7381 cgctccagtt caaacccgat gcggttgggt gaggcactct gcaccgcgaa cgtgctctct
     7441 gaaaatgacg aggcaatacc cgcgccatag atgcgcgtgc caccgccctg gcgcaccagc
     7501 ccaaactcca ccgtgtacca gtacacccgc gccagctcgg ccagcttgcc ccgcttctgg
     7561 gcccgcaagc caccctgccc gtaggcctgg atgaagtccg ctatcaccgg gtgcatcagc
     7621 atcggcacat ggccgaacac atcgtggaac acatcgggct cttccaggta atccagttcg
     7681 tgcgcaccgc ggatgaagtt gcccgccgga aaacgccggt tggccaggtg ctcaaagaac
     7741 acgtcatcgg gcaccagtcc tggaacagcg accacctgcc atcccgtggc gcggtgcagc
     7801 acctccgaca gcgcttcaaa gtcggggata cggtctgccg ccatcggtag cctgcgcatg
     7861 ccctcaatga atgcatcgca ggccaggcca ggcaggagcc gg
//
LOCUS       DAOSJM010000067         7559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8950_length_7559_cov_4.199360, whole genome shotgun sequence.
ACCESSION   DAOSJM010000067 DAOSJM010000000
VERSION     DAOSJM010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8950_length_7559_cov_4.199360"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKX82_03655"
     CDS             <1..485
                     /locus_tag="PKX82_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOF51375.1"
                     /translation="LVTVASGTCRALLSRPGTFPKPEGVEFALSAEASQYYKNGRPFL
                     QTYLPFWMANFVQRLGLMAVPLIALLLPFFRVVLPAISWWQKSRINRRYGELKFLEAE
                     IAKGQLTPEEIARAHQQLDHIEHEVTSSKFPLDFTDRIYTLRQHVGFVRDSLARQKAP
                     "
     gene            525..2954
                     /locus_tag="PKX82_03660"
     CDS             525..2954
                     /locus_tag="PKX82_03660"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOF51376.1"
                     /translation="MKTALKLMGALIALVLVIGLAAGGWYIYKKQPVRSGSVELTGLA
                     APVSVQYDERGVPHIRAENEADMYKALGYVHAQDRLFQMEIVRRLAKGELAEILGPKL
                     LETDKLFRTLGIRAHAQDYVARMDMNAPSAKGLLAYLDGINQYQATHKAPIEFDVLRI
                     PKRPFTPEDSIAVTGYLAYSFAAAFTTEPVMTFIRDKLGPQYLTAFDIDWHPEGVITP
                     LTAETIGKPEGVALSDRDWAGLNHLAQVSQEALDAAGVPRYEGSNAWAISGRRTASGK
                     PLLAGDPHIDYSAPSVWYEAHLSAPGFELYGHHQALAHVALLGHNTQFGWSLTMFQND
                     DVDLIAEKVNPDNANQVWFRDGWVDMTTRTETIQVKDQGPTHLKLRISPHGPIITDAY
                     KDNYGRTPIAMWWAFLQTENPILEAFYELNRANTREKARNAVSKIHSPGLNVVWANTA
                     GDIGWWAGGKLPQRPEGVNPMFILDAAKGEAEKPGFYAFNFNPQEENPARGYIVSANH
                     QPQPASGVPVPGYYNLPDRARRIDDVLKDPKRKWNIAGAQELQLDVSNGYFTRILKEL
                     LPVLEQVVTDPNEKAFLEPLQKWDGFYTRDSVAATLFSQLMYELAKATFEDELGEVQF
                     KNLLRTRALDLGLPVLVADAKSPWWDNVNTPQSESRFETARIAWAATYKHLSGLYGST
                     LLDWRWGNAHTLTHGHPLGMQKPLDKLFNVGPFQVPGGRETPNNYSGSVGPAPWKVKY
                     GPSTRRVIDFAAPDKAVGGNPVGQSGVLFDRHYQDQAERFAEGIYEEQYLSERDVQAH
                     TRSTLTLEPAR"
     gene            complement(2965..3669)
                     /locus_tag="PKX82_03665"
     CDS             complement(2965..3669)
                     /locus_tag="PKX82_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOF51377.1"
                     /translation="MLDLSKIKAITLDLDDTLWPILPTIERCERLLHTWLQEQAPATA
                     LLLTDPHVRRELRETTAQQWPDLAHDLASLRREAIRLALQRAGDAPALAEPAFAVFHA
                     ARMQVDLFEDARPGLASLAQRYPILALSNGSADVHQVGIGEYFKGSVSARDAGVSKPD
                     ARIFQLAAQTLGVAVCDVLHVGDDAMMDVVGALNAGMQTVWVNRADHLWSQPQHPHAT
                     VASMTELCDLLEVPAS"
     gene            3689..4780
                     /gene="hemH"
                     /locus_tag="PKX82_03670"
     CDS             3689..4780
                     /gene="hemH"
                     /locus_tag="PKX82_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HOF51378.1"
                     /translation="MSFSQEPPYSHGQPQRTAVLLCNLGTPDEPTPKAVRRYLAEFLS
                     DHRVVEIPRLVWMLILHGIILRVRPAKSAAKYASIWTPEGSPLKVWTERQAKMLQGWL
                     GQRNHQVKVRWAMRYGSTSITSQLDALKAEGVTRVLVVPAYPQYSATTTASVFDAVYG
                     WAQKTRALPELRFVNHYHDDADYIAALAASVQRHWKANGRPDQLVMSFHGVPERTLHL
                     GDPYHCECFKTARLLAQRLGLGKEQYTVTFQSRLGRAKWLEPYTEPTLIAMGQAGVER
                     VDVICPGFTSDCLETLEEINMEAREAFLHAGGKEFHYIPCLNDDPEWITALCNLTQRH
                     LQGWPTQETQDLRELEASRLAALELGAKQ"
     gene            complement(4851..5426)
                     /locus_tag="PKX82_03675"
     CDS             complement(4851..5426)
                     /locus_tag="PKX82_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOF51379.1"
                     /translation="MPQPSVAPRFFSTAQAFREWLERHAATEAELIVGFHKVATGQSC
                     MTWSESVDEALCFGWIDGVRKRIDDDAYQIRFTPRKPTSIWSAINIAKVQALQTQGRM
                     TPAGERAFALRADHRSAVYSHEQPAVAEWSTAELAAFRARKRAWVYWESTPPGYRKQM
                     QHRVVSAKRVETRAKRLQVLMDACAAGLRLR"
     gene            complement(5434..5913)
                     /locus_tag="PKX82_03680"
     CDS             complement(5434..5913)
                     /locus_tag="PKX82_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF51380.1"
                     /translation="MTPAKPLIALRAEDAPPRTKPSNYPEPFASMMAGRLKRPLGDLF
                     GLSNFGVNLTRLAPNSVSALRHAHTKQDEFIYVLQGRPVLITDEGRTQLEPGMCTGFK
                     AGTGNGHALVNESADEVVYLEVGDRTPGDAATYPDDDLQATLVEGSWKFAHKDGTPY"
     gene            complement(5910..6299)
                     /locus_tag="PKX82_03685"
     CDS             complement(5910..6299)
                     /locus_tag="PKX82_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008168852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOF51381.1"
                     /translation="MFSHIFIGVSDFDRALAFYNPLMTALGIAPRFCERDRPWAGWQS
                     TPGPRPLFLIGKPYNEQESQPGNGQMVAFLAETRAIVDQAHAVALQHGGTSEGAPGLR
                     PEYHAHYYGAYFRDPDGNKLCVACHTP"
     gene            complement(6326..6709)
                     /locus_tag="PKX82_03690"
     CDS             complement(6326..6709)
                     /locus_tag="PKX82_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOF51382.1"
                     /translation="MLKRIHHAAIICSNYEASKHFYVECLGFKVIAENFREQRNSYKL
                     DLALPDGSQVELFSFPEAPQRQSYPEARGLRHLAFVVDDVGACKMTLESRGIAVESIR
                     TDEYTGKRFVFFADPDGLPLELYEE"
     gene            complement(6723..>7559)
                     /locus_tag="PKX82_03695"
     CDS             complement(6723..>7559)
                     /locus_tag="PKX82_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF51383.1"
                     /translation="AGREIHYTQWGVGHPKVVIAWHGLARTGRDMDELAAHLSERGYR
                     VICPDTIGRGLSAWSPLPKEEYCLAFYAQIAADLFRQLGIEQAHWVGTSMGGAIGTVC
                     ASGLVVPDMQQRILSLTLNDNGPQLADAAIARIRSYAGSPPAFDTVLELEAFFRQVYK
                     PYGWLSDAQWRRLTETSTRRLPDGRVTPHYDPAMVEQFISHPNDYLIWNHYDAVQVPV
                     LCLRGENSDLLLPETVQAMRERGPGAAGRLTVVEVPDCGHAPALNVPAQLDCVTEFIT
                     KA"
BASE COUNT         1484 a         2503 c         2275 g         1297 t
ORIGIN      
        1 cccttgtgac tgttgccagc ggcacctgcc gagcgctgct gtcgcgcccc ggcactttcc
       61 ccaagccgga gggcgtggag tttgcgctgt ccgccgaggc cagccagtac tacaagaacg
      121 ggcgcccttt cctgcaaacc tatctgccgt tctggatggc caacttcgtg cagcgcctgg
      181 gcctgatggc tgtgccgctg attgccttgc tgctgccctt cttccgtgtg gtgctgcccg
      241 ccatcagttg gtggcagaaa agccggatca accggcgcta cggcgagctc aagtttctgg
      301 aagccgaaat tgccaaaggc cagctcaccc ccgaggagat cgcccgggcc caccagcagc
      361 tggaccacat cgagcacgaa gtcaccagct ccaaatttcc gctggacttc accgaccgca
      421 tctacacact gcgccagcat gtgggctttg tgcgtgatag ccttgcacgc cagaaggcgc
      481 cctgacggca gccccaacaa caagaaaacc gaggaagacc ccgtatgaaa accgcattga
      541 aactgatggg tgcactcatt gcgctcgtgt tggtcattgg cctggccgca ggcggctggt
      601 acatctacaa gaagcagccg gtccgcagcg gctcggtgga actcaccggg ctggcggcgc
      661 cggtgtcggt gcagtacgac gaacggggcg tgccccatat ccgggcggaa aacgaagccg
      721 acatgtacaa ggcgctgggc tatgtgcacg cgcaagaccg cctgttccag atggaaatcg
      781 tccgccgcct ggccaagggt gagctggccg aaattctggg ccccaaacta ctggaaaccg
      841 acaagctgtt tcgcacgctg ggcatccgtg cccatgcgca ggactatgtg gcccgcatgg
      901 acatgaatgc ccccagcgcc aaaggtctgc tggcgtatct ggacggtatc aaccagtacc
      961 aggccacgca caaggcaccg atcgagttcg atgtgctgcg catccccaag cgccccttta
     1021 cgcccgaaga cagcattgct gtcaccggct atctggccta cagctttgca gctgcgttca
     1081 ccaccgagcc ggtgatgact ttcatccgcg acaagctggg cccacagtac ctcactgcct
     1141 tcgacatcga ctggcatccc gaaggtgtca tcacgccgct gaccgcagag accatcggca
     1201 aacccgaagg tgtggccctg agcgaccggg actgggctgg tttgaaccac ctggcgcaag
     1261 tcagccagga ggcgctggac gctgccggcg tgccccgcta cgagggcagc aacgcctggg
     1321 ccatctcggg caggcgcacc gccagtggca agcccctgct ggccggcgac ccgcacattg
     1381 attactcggc accgtccgtc tggtacgagg cccacctcag cgcgcctggc tttgagttgt
     1441 acggacacca ccaggcactg gcccacgtgg cgctgctggg ccacaacacg cagtttggct
     1501 ggagcctgac catgttccag aacgacgacg tggacctgat cgccgagaag gtgaaccccg
     1561 acaacgccaa ccaggtctgg ttccgcgatg gctgggtgga catgaccacc cgcaccgaaa
     1621 ccatccaggt gaaagaccag ggcccgacgc acctgaaact gcgcatctcg ccccacggtc
     1681 ccatcatcac cgacgcctac aaggacaact acggccgcac cccgattgcc atgtggtggg
     1741 ccttcctgca gaccgagaac cccatcctcg aagccttcta tgaactcaac cgcgccaaca
     1801 cccgcgagaa ggcgcgcaat gctgtcagca aaatccattc ccccggcctc aacgtggtgt
     1861 gggccaatac cgcaggcgac attggctggt gggccggtgg caagctgcca cagcgacccg
     1921 agggtgtgaa ccccatgttc atcctggacg cagccaaagg cgaggccgag aagcctggct
     1981 tctacgcctt caacttcaat ccgcaggaag aaaaccccgc gcgcggctac atcgtctcgg
     2041 ccaaccacca gccccagccc gccagcggtg tgccggtgcc cggctactac aacctgcccg
     2101 accgcgcccg ccgcatcgat gatgtgctca aagaccccaa acgcaaatgg aacatcgccg
     2161 gcgcgcagga gcttcagctc gacgtgagca acggctactt cacccgcatc ctcaaggaac
     2221 tgttgccggt gctggagcag gtggtgaccg accccaacga aaaggctttt ctggagccac
     2281 tgcagaagtg ggacggcttc tacacccgcg acagcgtggc cgccacgctg ttcagccagc
     2341 tgatgtacga gctggccaag gccacttttg aagacgaact gggcgaagtg cagttcaaga
     2401 acctgctgcg cacccgcgcg ctggacctgg gcctgccggt gctcgtggcc gatgccaaat
     2461 cgccctggtg ggacaacgtc aacacgcccc agagcgagag ccggtttgag accgcacgca
     2521 ttgcctgggc cgccacctac aagcacctca gcggcctgta tggcagcacc ctgctggact
     2581 ggcgctgggg caatgcccac accctgacac acggccatcc gctgggtatg caaaagccat
     2641 tggacaagct attcaacgtc gggccgttcc aggtgcccgg tggccgtgag acgcccaaca
     2701 actattccgg cagcgtgggc cccgccccct ggaaggtcaa atacggcccc tccacccggc
     2761 gtgtgatcga ctttgccgcg cccgacaaag cggtgggtgg caaccccgtg ggccagagcg
     2821 gcgtgctatt cgaccggcac taccaggacc aggccgaacg ctttgccgaa ggcatctatg
     2881 aagagcaata cctcagcgaa cgcgacgtcc aggcccacac ccgaagcacg ctgacgctgg
     2941 agcctgcgcg ttagaccgtt tccgttacga agccggtact tccagcaggt cgcacaactc
     3001 cgtcatgctg gccaccgtgg catgtggatg ctgcggctgg ctccacaggt ggtctgcgcg
     3061 gttgacccac acggtctgca tgccggcgtt gagtgcccca acgacatcca tcatggcgtc
     3121 gtcccccacg tgcaagacgt cgcacaccgc cacacccagt gtctgtgcgg ccagctggaa
     3181 tatgcgcgca tcgggcttgc tcaccccggc gtcacgtgcg ctcacactgc ccttgaaata
     3241 ttcgccaatc cccacctgat gcacatcggc actgccgttg gacagggcca atatcggata
     3301 acgctgcgcc agcgaggcca ggcccgggcg cgcatcctca aacaggtcca cctgcatgcg
     3361 cgcggcgtgg aacaccgcaa acgccggctc tgccaacgcg ggcgcgtccc ccgcgcgttg
     3421 caaggccagg cggatggctt cgcgccgcag tgaggccagg tcgtgcgcca ggtccggcca
     3481 ctgctgcgcc gtggtttcgc gcagctcgcg ccgcacatgg ggatcggtca gcagcagcgc
     3541 cgtcgccggc gcctgttcct gaagccacgt gtgcaataac cgctcgcaac gctcgatggt
     3601 gggcaggatg ggccacagcg tatcgtcgag atcgagggta attgccttga ttttggaaag
     3661 gtccagcata ggtgcgggag aataccgcat gtctttctcg caagaacccc cctactccca
     3721 cggccagccg caacgcaccg ccgtactgct gtgcaacctg ggcacacccg atgaacccac
     3781 gccgaaggcg gtacgccgct acctggccga gtttttgagc gaccaccggg tggtggaaat
     3841 cccccgcctg gtgtggatgc tgatcctgca cggcatcatc ctgcgcgtac gcccggccaa
     3901 gtcggccgcc aagtacgcca gcatctggac acccgagggc tcgccactca aggtgtggac
     3961 cgagcgccag gccaagatgc tgcagggctg gctgggccag cgcaaccacc aggtcaaggt
     4021 gcgctgggcc atgcgctacg gcagcacgtc catcacctcg cagctcgacg ccctgaaggc
     4081 cgagggcgtg acccgcgtgc tggtggtgcc tgcctacccg cagtacagcg ccaccaccac
     4141 cgccagcgtg ttcgacgccg tctatggctg ggcgcagaag acccgcgcac tgcctgagct
     4201 gcgctttgtc aaccactacc acgacgatgc cgactacatc gccgcactgg ccgccagcgt
     4261 gcagcgccac tggaaagcca atggccgccc ggaccagctg gtcatgagtt ttcacggcgt
     4321 gcccgagcgc accctgcacc tgggcgaccc ctaccactgc gaatgcttca agaccgcacg
     4381 cctgctggcc cagcgcctgg gcctgggcaa ggaacagtac acggtcacct tccagtcccg
     4441 cctgggccgc gccaaatggc tggagcccta caccgagccc acgctgattg ccatgggcca
     4501 ggccggtgtg gagcgcgtgg acgtgatctg ccccggcttc accagcgact gcctggaaac
     4561 actggaagaa atcaacatgg aagcgcgcga agcatttctg cacgccggag gcaaagaatt
     4621 ccactacatc ccctgcctga acgacgaccc cgagtggatt accgcgctgt gcaacctgac
     4681 ccagcgccat ctgcagggct ggccgacgca agagacgcaa gacctgcgcg aactcgaagc
     4741 ctcgcgcctg gctgcgctgg agttgggggc caagcagtag cccctggggt tgctacatat
     4801 ttaatagcta ctcgcgcatg ttccacgggg gctagaggcc aatttagctc ttaacgcagg
     4861 cgtaaaccag ctgcgcaggc atccatgagt acctgcagac gcttggcacg ggtctcgacg
     4921 cgtttggcag acaccacgcg gtgctgcatc tgcttgcggt agcctggcgg cgtgctttcc
     4981 caatagaccc acgcacgctt tcgggccctg aaagcagcca gctcggccgt gctccattcg
     5041 gccaccgcag gctgttcatg ggaatacacc gcggacctgt ggtcggcacg cagcgcaaaa
     5101 gcgcgctccc ccgccggcgt catgcggccc tgcgtctgca gcgcctggac cttggcgatg
     5161 ttgatggcgc tccagatcga tgtaggcttt ctgggcgtaa accggatctg gtaggcatcg
     5221 tcgtcaatgc gtttgcgcac cccgtcgatc cagccaaagc acagcgcctc gtccaccgac
     5281 tcggaccacg tcatgcacga ctgcccggtg gccaccttgt gaaagcccac gatgagttct
     5341 gcctcggtcg cagcatggcg ctccagccat tcccgaaagg cttgggcagt gctgaaaaag
     5401 cgtggggcaa cggaaggctg cggcacaggt gcgtcaatac ggcgtgccgt ctttgtgggc
     5461 gaatttccaa ctgccttcga ccagcgtggc ctgcaagtcg tcgtccgggt aggtcgccgc
     5521 atcgccaggc gtacggtcgc ccacctccag ataaaccacc tcgtcggcag actcgttgac
     5581 cagcgcgtgg ccgttgccgg taccggcctt gaaacccgta cacatgccgg gctccagctg
     5641 cgtgcggcct tcgtcggtga tgagcaccgg tctgccctgc aacacgtaaa tgaactcgtc
     5701 ctgcttcgtg tgcgcatggc gcagcgcaga caccgagttc ggcgccaggc gcgtgaggtt
     5761 gacgccaaag ttcgacagac caaacagatc acccagcggg cgcttcagcc tgccggccat
     5821 catcgatgcg aacggctccg ggtagttgga cggtttggtg cgtggcggtg cgtcttcggc
     5881 acgcagggcg atgaggggtt tggcaggtgt catggtgtgt ggcaggcaac acagagcttg
     5941 ttgccatcag ggtcccggaa ataggcgcca tagtaatgcg cgtggtactc gggccgcaaa
     6001 ccgggcgccc cttcggacgt gccaccgtgt tgcagtgcga cggcatgggc ctggtccacg
     6061 atggcgcggg tctctgcgag aaacgcgacc atctgaccgt tgccgggctg cgactcctgt
     6121 tcgttgtaag gcttgccgat gaggaacagt ggcctgggcc ccggagtcga ttgccagcct
     6181 gcccacgggc gatctctttc gcaaaagcgc ggcgcgatgc cgagcgcggt catcaagggg
     6241 ttgtagaaag ccagtgcgcg gtcgaagtca ctgacgccga tgaagatgtg ggaaaacatg
     6301 actcaactcc accaacggtc tgaatctact cttcgtaaag ctccagaggc aatccgtcgg
     6361 gatcagcaaa aaatacaaag cgcttacccg tgtactcgtc ggtgcgtatg ctttcgacag
     6421 caatacccct cgattccagc gtcattttgc aggcaccaac atcgtcaaca acaaacgcca
     6481 ggtgacgcag tccccgtgcc tcgggatagg actgccgttg gggcgcctct ggaaatgaaa
     6541 agagttcgac ctggctccca tctggaagtg caagatccag tttgtacgaa ttgcgctgtt
     6601 cccgaaaatt ctcggcaatc accttgaaac cgaggcattc gacatagaag tgcttcgaag
     6661 cttcgtagtt ggagcaaatg atcgctgcgt gatgaatgcg tttcagcatg ctgaatgctc
     6721 gcctaagcct tcgtgatgaa ctctgtcacg caatcgagct gtgccggaac attcaatgct
     6781 ggtgcatgcc cgcaatccgg cacctccacc accgtcaacc tacccgccgc acccggccca
     6841 cgttcacgca tggcttgcac ggtctctggc agcagcaggt cagagttctc accgcgcagg
     6901 cacagcacgg gcacctgcac tgcgtcgtag tggttccaga tgaggtagtc gttggggtgg
     6961 ctgatgaact gctccaccat ggccgggtcg tagtgcggcg tcacgcgccc gtcgggcagg
     7021 cggcgggtcg acgtttcggt caatctgcgc cactgtgcgt cgctgagcca gccgtagggt
     7081 ttgtacacct gccggaaaaa cgcctcaagc tccagtacgg tatcaaacgc cgggggtgag
     7141 cccgcataag agcggatacg ggcaatcgct gcatcggcca gttgtgggcc gttgtcgttg
     7201 agcgtgaggc tcaagatgcg ctgctgcatg tctggcacca ccaggcccga tgcgcacaca
     7261 gtaccaatgg cgccgcccat ggaggtgccc acccaatgcg cctgctcgat gcccagttgg
     7321 cggaacaggt ccgccgcaat ctgcgcataa aacgccaggc agtattcctc tttgggcagc
     7381 ggactccagg cgctcaggcc gcggccaata gtgtcggggc agatgacgcg gtaaccgcgc
     7441 tcgctcagat gcgcggcaag ctcatccatg tcccggccgg tgcgggccag gccgtgccag
     7501 gcaatgacca ctttcgggtg cccgacgccc cactgggtgt agtggatctc gcggccagc
//
LOCUS       DAOSJM010000068         7547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_8962_length_7547_cov_4.040577, whole genome shotgun sequence.
ACCESSION   DAOSJM010000068 DAOSJM010000000
VERSION     DAOSJM010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7547
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8962_length_7547_cov_4.040577"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PKX82_03700"
     CDS             complement(<1..278)
                     /locus_tag="PKX82_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51384.1"
                     /translation="MRSTLNIPDPDLPRAQRLWGRWPAVTVGVLLAHWGLLHALPSAT
                     IPLQPQDAQPVAMTFTTRTIMPVAQDVVRQEAPSRTVKTQAQPTEFKR"
     gene            364..1452
                     /locus_tag="PKX82_03705"
     CDS             364..1452
                     /locus_tag="PKX82_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOF51385.1"
                     /translation="MKLASYKDGSRDGQLVVVSRDLTQAAYASNVANRLQQVLDDWNY
                     LSPQLQDVYDALNAGRARHAFPFDPAQCMAPLPRAYQWADGSAYLNHVELVRKARGAE
                     VPASFYTDPLMYQGGSDDFLGPQDPIVCASEAFGIDFEAEVAVITGDLPMGCAADRAI
                     DGIRLVMLANDVSLRNLIPDELAKGFGFVQSKPATAFSPVAVTPDELGESWAKGRVHL
                     SLQSTWNGRKVGMCDAGPEMTFHFGQLIAHLCKTRNVRAGSIIGSGTVSNKGVEKKGR
                     TDWPNGYSCIAEKRAMETIQDGQPSTEFMKYGDTIRIEMKGRDGLSVFGAIDQDVVPP
                     EVKVRPGRPTAAAIPDPSALNQTSETPE"
     gene            1449..2567
                     /locus_tag="PKX82_03710"
     CDS             1449..2567
                     /locus_tag="PKX82_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HOF51386.1"
                     /translation="MKTKAAVAWKAGQPLTIETVDLQGPKFGEVLVEIKATGICHTDY
                     YTLSGADPEGIFPAILGHEGAGIVVDVGPGVTTLKKGDHVIPLYTPECRQCKFCLSRK
                     TNLCQLIRGTQGKGLMPDATSRFSLNGEPIFHYMGTSTFSNYTVAPEISLAKIREDAP
                     FDKVCYIGCGVTTGIGAVIFTAKVEAGANVVVFGLGGIGLNVIQGAKMVGADKIIGVD
                     INPARQEMARKFGMTHFINPKEVQAAGGTVVDAIVQLTDGGADYSFECIGNTQVMRQA
                     LECTHKGWGRSIIIGVAEAGAEISTRPFQLVTGRKWEGSAFGGARGRTDVPKIVDWYM
                     EGKINIDDLITHTMPLEDINKGFDLMKRGESIRGVVIY"
     gene            2567..3430
                     /gene="fghA"
                     /locus_tag="PKX82_03715"
     CDS             2567..3430
                     /gene="fghA"
                     /locus_tag="PKX82_03715"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HOF51387.1"
                     /translation="MTTSTDFNLQSEHVCFGGVQRFYRHFSAEIGLPMGFGVYLPPQA
                     SKGPVPALVFLAGLTCTEETFAFKAGAQRVASELGLALITPDTSPRGAGIVGEADHWE
                     FGVGAGFYLDATQAPWSGHWCMESYLLRELLPRMSQELPLDGQRLGITGHSMGGHGAL
                     TLALRHPGMFKSLSALAPICAPTQCPWGKHAFTGYLGQDESTWAAHDASALMAAQTRA
                     PYPGGILVDQGLADKFLEVQLHPHLLEAACASVGQPLELRRHAGYDHGYYFISTFIED
                     HLRHHARALAA"
     gene            complement(3437..4039)
                     /locus_tag="PKX82_03720"
     CDS             complement(3437..4039)
                     /locus_tag="PKX82_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOF51388.1"
                     /translation="MNYTFWSATILLILITDPIGNIPIFANALKHVPPQRRPWVILRE
                     IAIAFGLLLTFMFVGESFLRVMNLSELALQIGGGVILFLIALRMVFPPPPGPDSEQLR
                     EPLIVPLAVPAIAGPSALATVLLLVSQQPDKRIEWIGALCVTMAISAIVLVSAERIQR
                     IIGDRLVTAVERLMGLVLVSVAIEMMLRGFKTFALQLARG"
     gene            complement(4039..4452)
                     /locus_tag="PKX82_03725"
     CDS             complement(4039..4452)
                     /locus_tag="PKX82_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOF51389.1"
                     /translation="MRSLFHFAFNVTDLDIARRFYGGVLGCAEGRSTATWVDFDFFGH
                     QLSLHLGQPFPTANTGHVGDHLVPMPHFGLVLALPDWQALAQRLTDAGTAFVLEPQLR
                     FAGQPGEQWILFLLDPFGNPIEIKGFQSLDTVYTP"
     gene            complement(4492..4851)
                     /locus_tag="PKX82_03730"
     CDS             complement(4492..4851)
                     /locus_tag="PKX82_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HOF51390.1"
                     /translation="MTKLDTTPFKAQLLEQRASLLGQLATLRGGDVTRAEASAEHFSR
                     REDSQAQTTTERELELTLDSRETTELDAVEAALQRIAAGTYGECTDCGVHIAAARLQA
                     TPEAPRCIHCQEKAEKA"
     gene            4970..5335
                     /locus_tag="PKX82_03735"
     CDS             4970..5335
                     /locus_tag="PKX82_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HOF51391.1"
                     /translation="MTLLQALRHSRLIARSVLAWFVLSMAVAVAAPVVQPHTSVLVCS
                     ASGAIQLVDTSSDDGVAQGSHTLDCVLCLALHAAPPVAVDLAASSKATSGPVGFRALA
                     PVAYRSDATLAARAPPALI"
     gene            5454..5912
                     /locus_tag="PKX82_03740"
     CDS             5454..5912
                     /locus_tag="PKX82_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HOF51392.1"
                     /translation="MKKSTLVAATLLATTLSIQAFAQTVEVKDAWARATVQGQMATGA
                     FMKLTAKEGSRLVAASSPVAGVTEVHEMAMDNNIMKMRAVPGLDLPAGKAVELKPGGY
                     HVMLMDLKAPLKKDTTIPLTLVFKDARGVESRTELKVPVNTAAPMAEHKH"
     gene            6016..6705
                     /locus_tag="PKX82_03745"
     CDS             6016..6705
                     /locus_tag="PKX82_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51393.1"
                     /translation="MSDSDTSGFGKFVPGFDFLQNLAKGATNNIPQMPNLGNWVAPTL
                     NVEELEKRIDELKAVHFWLEQNSRALGATIQALEVQKMTLATLKGMNFNIGDVANALK
                     LKAADSVYSGVQRVTEKAASTAKTISDVATSSTPAATAAKAVKGAMPAAGVAGLVDPL
                     NLWGSLTQQFQQIAAGAMKEATTKTAMDMTKHMATGMAKEAIKSAKAAGKKAVVKRTT
                     RKTAAKKTTAR"
     gene            6738..>7547
                     /locus_tag="PKX82_03750"
     CDS             6738..>7547
                     /locus_tag="PKX82_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOF51394.1"
                     /translation="MQLFPYGHATHPQWQMAAALVLAQVRAQMALHGYATAPTLALLY
                     ITDHYAAQAQDILEHLQAELPLVTDWSGTVGVGIVSNNVEYWDEPALSLMLLELPSDQ
                     HRVFSGVAPLGMGFEAHTALVHADGDAADLAELVEEMAARTDAGYVFGGVSSSRTRSL
                     QFAVGGNGNIRGHGAASGVFSGGLSGVAFGREVGLISRVTQGCMPVSSVHTVTGADHN
                     VAVGLDGEPALDVMLRDLKISLDQPQQALEVVRATLVGLVRSGEAAQPNGSL"
BASE COUNT         1511 a         2286 c         2378 g         1372 t
ORIGIN      
        1 attctcttga actccgttgg ctgagcctgg gtcttgacgg tacggctggg agcttcctgg
       61 cgcaccacat cctgcgccac cggcatgatg gtgcgggtgg tgaaggtcat tgctacgggc
      121 tgggcgtcct gtggttgcaa tggaattgtt gcagacggca aggcatgcaa caggccccag
      181 tgggccagca gtacgcccac ggtcaccgcc ggccaccggc cccacagacg ttgggcccgt
      241 ggcaagtcgg ggtcaggaat gttcagtgtg ctgcgcatgg attgggatgg ttagggacaa
      301 cagaatgtcc ctaacaacgt tttcagtgta agccacggta acaaaacacg cgaaaataag
      361 cccatgaaac ttgcaagcta caaagacggt tcccgcgacg gccagttggt tgtggtttcc
      421 cgcgacctga cccaggcagc ctacgcttcg aatgttgcca atcggctgca acaggtactg
      481 gatgactgga actacctgtc gccccaattg caggacgtgt acgacgccct caacgcgggt
      541 cgggcgcgcc atgcctttcc gtttgacccg gcacagtgca tggcaccgct gccgcgtgcc
      601 taccagtggg ccgatggttc ggcgtacctc aaccatgtgg aattggtgcg caaggcgcgt
      661 ggcgccgaag tgcctgcgag tttttacacc gatccactga tgtaccaggg tggtagcgac
      721 gattttctcg gaccccagga ccccatcgtg tgcgccagcg aggcgttcgg tatcgacttc
      781 gaagcggaag tggcggtcat caccggcgac ctccccatgg gctgcgcggc ggaccgggcc
      841 attgatggca tccgtctggt catgctggcc aatgatgtga gtctgcgcaa cctcattccc
      901 gacgagctgg ccaaagggtt tggctttgtg cagagcaagc ccgccacggc cttcagcccg
      961 gtggcagtca cgcccgacga gttgggtgaa tcctgggcca agggccgggt gcacctgagt
     1021 ctgcaaagca cctggaacgg ccgcaaggtc ggcatgtgcg acgccggtcc ggagatgaca
     1081 ttccacttcg gtcagctcat cgcccacctg tgcaagaccc gcaatgtacg cgccggttcc
     1141 atcattggtt caggcaccgt gagcaacaag ggcgtggaga aaaaagggcg gacggactgg
     1201 cccaacggct acagctgcat cgctgaaaag cgcgccatgg aaaccatcca ggatggacag
     1261 cccagcaccg aattcatgaa gtacggggac accatccgca ttgagatgaa ggggcgcgat
     1321 ggtctcagcg tgtttggtgc catcgaccag gatgtggtgc cacccgaagt caaggtgcgg
     1381 cccggtcgcc cgacagcggc cgctatccct gaccccagcg cattgaacca aaccagcgag
     1441 accccagaat gaaaaccaaa gccgccgttg cctggaaagc aggccaacca ttgaccattg
     1501 aaaccgtgga tctgcagggc cccaagttcg gggaagtgct ggtggaaatc aaggccaccg
     1561 gcatctgcca tacggactac tacacgctca gcggtgccga ccctgaaggc atcttccccg
     1621 ccatcctggg ccacgaaggc gctggcattg tggtggacgt gggtcccggt gtgaccacgc
     1681 tcaagaaggg cgaccacgtc attccgctct acacccccga atgccgccag tgcaaattct
     1741 gtctatcgcg caagaccaac ctgtgccagc tgatccgtgg cacgcagggc aagggtctga
     1801 tgcctgacgc caccagccgc ttcagcctga atggcgagcc gatcttccat tacatgggca
     1861 cgtccacgtt cagcaactac accgtcgcac ctgaaatttc actggccaag atccgtgaag
     1921 acgctccgtt cgacaaggtc tgctacatcg gctgcggcgt caccaccggc atcggcgcag
     1981 tgatcttcac cgccaaggtg gaggcgggtg ccaacgtggt cgtattcggg ctgggcggca
     2041 ttgggctgaa cgtgattcag ggcgcgaaga tggtgggcgc cgacaagatc attggcgtgg
     2101 acatcaaccc cgcacgccag gagatggcac gcaagttcgg tatgacgcac ttcatcaacc
     2161 ccaaggaagt acaagcagcg ggtggtacgg tcgtcgacgc catcgtgcaa ctcaccgacg
     2221 gcggtgcgga ctacagcttt gaatgcattg gcaacaccca ggtcatgcgc caggcgctcg
     2281 aatgcaccca caagggctgg ggccgcagca tcatcattgg cgtggccgag gccggtgctg
     2341 aaatctctac ccgcccattc cagctcgtca cgggccgcaa gtgggaaggc tcggcctttg
     2401 gtggcgcccg cggccgcacc gatgtgccca aaattgtgga ctggtacatg gaaggcaaga
     2461 tcaacatcga cgacctgatc acccacacca tgccgctcga agacatcaac aaggggttcg
     2521 atctgatgaa gcgcggcgag tcgatccgtg gtgtggtgat ttattgatga ccacttcgac
     2581 ggacttcaac ctgcaaagtg agcacgtctg ttttggtggc gtgcagcgct tttaccggca
     2641 tttctccgca gagatcgggc ttcccatggg cttcggggtg tatctgcctc cgcaagccag
     2701 taagggtcct gtgccggcgc tggtgttcct ggccgggctg acctgtaccg aagaaacctt
     2761 tgctttcaag gcaggagccc agcgcgtcgc atccgaactg gggctggcgc tgattacgcc
     2821 cgacaccagt ccgcgcggcg ccggcattgt gggcgaagcc gaccattggg agtttggcgt
     2881 gggcgccggc ttttatctgg atgccaccca ggcgccgtgg agcggccatt ggtgcatgga
     2941 aagctacttg ctgcgtgagc tgctgccgcg catgtcgcag gaattgccgc tggatggcca
     3001 acggctcggg attaccggcc attccatggg cggacacggc gcactgacgc tggcgctacg
     3061 gcatcccggc atgttcaagt cgctgtcagc gctggcaccg atttgcgcgc caacgcaatg
     3121 cccctggggc aagcatgcat ttaccggcta cctggggcag gacgaaagca cctgggccgc
     3181 ccacgatgcc agtgctctga tggctgcgca aacgcgcgcg ccgtaccccg gcggcatact
     3241 ggtggaccag gggctggccg acaagtttct ggaggttcaa ctgcatccgc acttgctgga
     3301 agcggcctgt gccagtgtgg gccagccatt ggaattgcgt cgccatgcgg gctacgacca
     3361 tggctactat ttcatcagca ccttcatcga agaccacctg cgccaccatg cgcgggcgct
     3421 agcagcctga gagggctcag ccgcgggcca gttgcagcgc gaaggtcttg aaaccgcgca
     3481 gcatcatttc aatcgccacc gacaccagca ccagtcccat cagccgctcc accgcggtga
     3541 cgagtcgatc gccaatgatg cgttgaatac gctcagccga caccagcacg atggcgctga
     3601 tagccatggt gacgcacagc gcgccaatcc actcgatacg cttgtcgggc tgctgcgata
     3661 ccagcaacag caccgtcgcc agggctgacg gcccagcaat cgcgggcaca gccagcggca
     3721 cgatcagcgg ttcccgcaac tgctcggaat ccggtccagg cggtggggga aacaccatgc
     3781 gcagtgcgat caggaacagg atcacgccgc cgccaatctg cagggccagc tcgctgagat
     3841 tcatcacccg cagaaagctc tcgcctacaa acatgaaggt cagcagcagt ccaaatgcga
     3901 tggcgatctc gcgcaggatc acccaggggc gccgttgggg gggaacgtgt ttgagcgcgt
     3961 tggcaaagat cgggatattt ccgatcgggt cggtgatcag aatcagcagg atggttgctg
     4021 accaaaaggt gtagttcatc atggtgtgta aaccgtatcg agcgattgga accccttgat
     4081 ctcaatcgga ttgccaaacg ggtccagaag aaacaggatc cattgttcgc caggctggcc
     4141 ggcaaaacgc agctgtggct ccagtacaaa ggccgtgccc gcatccgtga ggcgctgggc
     4201 gagggcctgc cagtccggca gtgccagcac caggccaaag tggggcatgg ggaccaggtg
     4261 gtcgcctaca tggccggtgt tggcggtggg aaaaggctgg cccagatgca gcgagagctg
     4321 gtggccaaag aaatcaaagt cgacccaggt cgcagtgctg cgaccctcgg cgcatcccaa
     4381 cacgccgccg taaaaacggc gtgcgatgtc caggtcggtg acgttgaaag cgaaatgaaa
     4441 caaactgcgc atgcgcgcag gatagcagcc ccgcaggccg ctttccgccg gtcaggcctt
     4501 ttcggctttt tcctggcagt ggatgcaacg gggcgcttca ggcgtggcct gcaggcgtgc
     4561 tgcagcaata tgaacaccgc agtcggtgca ctcgccgtag gtgccagccg caatgcgttg
     4621 gagtgcggct tccactgcgt ccagttcggt ggtttcgcgg gaatccagtg tcagttccag
     4681 ctcgcgctcg gtggtggtct gggcctggga gtcttcgcgg cgcgagaagt gctctgccga
     4741 tgcctcggca cgggtcacgt caccgccgcg cagggtggcc agctgaccca gcagcgatgc
     4801 acgctgttcc agcagttggg ctttgaaggg ggtggtgtcg agcttggtca tggtgcagtc
     4861 agtcaatgga ttggaaatac aagtatccat catgcgcgga cgcgcgtcga tggtctttga
     4921 ctgaggtcaa gctttggcag cgtccggaaa tgggctgata atgcggccga tgacgctttt
     4981 gcaagccctt cgccactcca gactgatcgc ccgctccgtg ctggcgtggt ttgtgttgtc
     5041 gatggcggtt gccgttgcgg cacccgtggt gcagccgcac accagcgttc tggtgtgttc
     5101 ggcgtctggt gccatccagc tggtggatac cagcagtgat gatggcgtgg cacaaggcag
     5161 ccatacgctg gattgcgtgt tgtgtctggc actgcacgca gcgcctccgg tggcagtcga
     5221 tcttgcagct tcgtccaagg caacttctgg ccctgtaggc ttccgggcac tggcgcccgt
     5281 ggcttatcgc agcgatgcca cattggccgc gcgcgctccc cccgctctga tctgaaccgg
     5341 cttcgcgccg acatccccgc cggttagctg caggtccttt gacttgccgt gctgcacatc
     5401 cgtcccgcgg gcggtcacgc ccactgaaac ccatttttta ccaaggaaga cccatgaaaa
     5461 aatcgactct cgttgctgcc accctgctgg ccaccactct ttccatccag gcatttgccc
     5521 agaccgtcga agtgaaagac gcctgggcac gcgccaccgt gcaaggccag atggccaccg
     5581 gcgcgttcat gaagctcacc gccaaagaag gctctcgcct ggtagccgct tcgtcgccgg
     5641 ttgcgggtgt gacggaagtg catgaaatgg ccatggacaa caacatcatg aaaatgcgtg
     5701 ccgtccccgg tctggacctg ccagcaggca aagctgtgga actcaagcct ggcggctacc
     5761 acgtcatgct gatggacctg aaggcacccc tgaagaagga caccacgatc cccctgaccc
     5821 tggtgttcaa ggatgccagg ggtgtggaaa gcaggaccga actcaaggtg ccggtgaaca
     5881 cggctgcgcc gatggccgag cacaagcact gaaccactgc cgctgcaccg ctgctgcgtg
     5941 ttttccgggg tgtccagaag gagtacagtg gagtaagctg catgtagcag agataaccac
     6001 ccctttggag acaccatgag cgattcagac accagcggtt ttggcaagtt cgtccccggt
     6061 tttgactttc tgcaaaacct ggccaaaggg gccaccaaca acatcccgca gatgcccaac
     6121 ctgggcaact gggtggctcc cacgctgaat gtggaagagc tggaaaagcg cattgacgaa
     6181 ctcaaggcgg tgcatttctg gctggagcag aactcgcgcg cgctgggtgc caccatccag
     6241 gcactggaag tgcagaagat gacactggcc accctcaaag gcatgaattt caacatcggg
     6301 gacgtggcca acgcgctcaa gctgaaggcg gcggattcgg tgtactccgg tgtgcagcgc
     6361 gtgacagaga aggcggcgtc caccgccaag accatatcgg acgttgctac cagcagcacg
     6421 cctgcagcta ccgcagccaa ggcggtcaag ggcgccatgc ccgctgcggg tgtggccggg
     6481 ctggtcgacc cgctgaacct gtggggttca ttgacccagc agttccagca gattgcagcg
     6541 ggcgccatga aggaagccac caccaagacc gccatggaca tgaccaaaca catggccacc
     6601 ggcatggcca aggaagccat caagtccgcc aaagccgcag gcaagaaagc ggtggtcaaa
     6661 cgcaccacgc gcaagacagc ggccaaaaag accactgcgc gctaaacgca gtctgaacca
     6721 tcggcccagg tcaggccatg caactcttcc cctacggcca cgccacccat ccgcaatggc
     6781 agatggcggc tgccttggtg ctggcccagg tgcgcgcaca gatggctctg catggctatg
     6841 ccacggcccc cacgctggcc ttgctttaca tcaccgacca ctatgccgcg caggcgcagg
     6901 acattctgga gcacttgcag gcagagctgc cgctggtgac cgactggtct ggcacggtgg
     6961 gtgtgggcat tgtgtccaac aacgtggagt actgggatga acccgccctg tccctgatgc
     7021 tgctggaact tcccagcgac cagcaccgtg tgttctccgg tgtggctccg ctgggtatgg
     7081 ggtttgaggc gcacaccgcg ctggtccatg ccgatggcga cgccgcggac ctggccgaac
     7141 tggtggaaga gatggcagcc cgcaccgatg cgggctatgt gtttggaggc gtctcctcca
     7201 gccgtacccg cagcctgcag tttgctgtgg gcggcaacgg caatatccgt ggccatggag
     7261 cggccagcgg cgtcttcagc ggcgggctga gcggtgtggc gttcggtcgt gaagtcggcc
     7321 tgatctcgcg ggtgacgcag ggctgcatgc cggtgtcttc ggtgcacacc gtgaccgggg
     7381 ccgaccacaa tgtggcggtg ggcctggatg gggagccggc actggacgtg atgctgcggg
     7441 atctgaaaat ctcgctcgac cagcctcaac aggctctgga agtggtgcgc gctacgctgg
     7501 tagggctggt gcgctccggt gaggccgccc agcccaatgg ttcgctg
//
LOCUS       DAOSJM010000069         7506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9020_length_7506_cov_4.622735, whole genome shotgun sequence.
ACCESSION   DAOSJM010000069 DAOSJM010000000
VERSION     DAOSJM010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9020_length_7506_cov_4.622735"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="PKX82_03755"
     CDS             complement(<1..822)
                     /locus_tag="PKX82_03755"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOF51395.1"
                     /translation="MKTAPSPTPVATPAKRSKDNERPLVEDIRLLGRILGDVIREQEG
                     VEAFELIEQIRKLSVAFRRDADHEADKALKKLLKGLSGDQTVSVIRAFTYFSHLANLA
                     EDRHHIRRREIHERAGDTQEGSIEVAMSRLRWAGISPKTISNTLAHSYVSPVLTAHPT
                     EVQRKSILDAERDIAQLLTARDELKLRAAMNTGKKDALSPRELAANEGHMRARVMQLW
                     QTRLLRFSKLTVADEIENALSYYESTFLREIPKLYADLEEKLGNHPVHSFLRMGQW"
     gene            876..1796
                     /gene="hemC"
                     /locus_tag="PKX82_03760"
     CDS             876..1796
                     /gene="hemC"
                     /locus_tag="PKX82_03760"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOF51396.1"
                     /translation="MQHFTIATRESRLALWQAEHVKALLEQHGHKVSLLGMTTQGDQI
                     LDRALSKVGGKGLFVKELEVALEEGRADLAVHSLKDVPMELPAGFTLACVMEREDPRD
                     AWVSSKYATLLDLPQGAVVGTSSLRRMALVRALRPDLKIEALRGNLDTRLRKLDDGLY
                     DGIVLAAAGLKRLGLQSRIRAIFEPQQMLPAAGQGALGIEVRSDRADVIAALAPLMHQ
                     STWLAVSAERAVSRVMGGSCSMPLAAHAVFSGDVLKINAAWGDPDGQMPLVQTQALAS
                     VSDTAAAVRLGEQVAAALQAGVEQATGKAH"
     gene            1799..2590
                     /locus_tag="PKX82_03765"
     CDS             1799..2590
                     /locus_tag="PKX82_03765"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOF51397.1"
                     /translation="MRVILTRPEREARQWVGALAQAGMDVISMPLIAIDGPPDPPAVV
                     RAWQGVEQLDAVMFVSGNAVDHFFALKPPLAPVFVAQSAIKTRAFVTGPGSREALLRN
                     GVDVACIDAPDAQAGQFDSEALWAVVQQRVHPGFRVLIVRGADSADARSEGAGRDWFT
                     RQVKAAGGQVELVASYQRRCPTLTPAELATARGAATDGSVWLFSSSEAVANLNSLLPD
                     QSWQQARAVATHPRIVQAAHIAGFGVVCESRPVLGAVVASIESLQ"
     gene            2587..3651
                     /locus_tag="PKX82_03770"
     CDS             2587..3651
                     /locus_tag="PKX82_03770"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOF51398.1"
                     /translation="MNSEPAAPSDTPSAASVPMPEVALQSEPVKASSRWLWAVGLVTA
                     GGLLGSVMTWQKLGNIQEQLARQSADTGAQATEARANAKQAQELAMETAAKLAVTDAR
                     VSEVSLQRTQLEELMQSLSRSRDENMVVDIESAVRLAQQQAQLTGSVEPLLAALKTAD
                     QRIARAAQPRLTPLQRAIAKDVARIKGTALADTPALLVKLDELVLLADELPVGNAVGA
                     TRTAPQLQRKPEETVGSWWIRMGLLVRDELRSLVRVSRIGDPDAALLSPEQSFFVREN
                     LKLKLLNAKLGLLSRQIDSARADLAAAGASMGRYFDANSRKTQTAAVLLQQVQSQMRA
                     LEVPRVDETLAALSTAAAGR"
     gene            3654..4937
                     /locus_tag="PKX82_03775"
     CDS             3654..4937
                     /locus_tag="PKX82_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HOF51399.1"
                     /translation="MRFALWLMALFAVATASALFAGNNQSTVTLFWAPYRVDLSLNLV
                     LLILLAMFVVLHLALRAINATLRLPQQARRWRLQQKERAIYSALLDSLSHLVAGRFIR
                     ARKSAELVVSLEDSVTRGGESLVHVGRLRAITHLLAAESAHALQDKPVRDSHFQQALQ
                     HAAQRDVQDVRDGLHLRAARWAFDERDATAALEWLDQLPQGASRRTLALRLRFKAARL
                     AGQNREALEVVRLLTKHRAFSEGAGRSIARGLAIELVRSAHDPVQLTRAWDALDTAER
                     LMPDVAIEATERLLQLGGEAPTARQWLLPVWSAMTGSSDALALVQRVRLVRVLEKSFG
                     KGDGAPDTTWLGRIESAQMANPRDAALQYLAGVVCMRLQLWGKAQQLLKQSLSMLQDN
                     GLKRDAWRALAEMAEQRQDMQAATEAYREALKLPV"
     gene            5001..6077
                     /locus_tag="PKX82_03780"
     CDS             5001..6077
                     /locus_tag="PKX82_03780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF51400.1"
                     /translation="MPTLIEHLIKLTDHRDRDLLELTLSKALIGLLPIERVVMVRVVS
                     EEGVRRWLEIASLDARGGGKVVDPLRVDFNVLRPVEDDKDRLHCVQSRSNVEVAWAGE
                     DGPRITYLPLFNDSRNDDEGVVEVHSGSPLTNEQFLTIGAVLHVFRNMYNLLAYSDRD
                     ALTGLLNRKSLDDTFYSAVLEELEEGKASRMQELAAPIEPGKERRHCVPPNYWLGTVS
                     VDHFDVLGNKNGHLIAEEVLLLVARIMNNTFRTYDRIYRFGGEQFAVLMHCPDEALVL
                     AAFERFRANMERFNFPQVGRVTASAGFTRISADDSPSTALERAERAVEYAQHHGHNKV
                     FSHGDLVRKGLLDGATRTGAVDLF"
     gene            complement(6098..6754)
                     /locus_tag="PKX82_03785"
     CDS             complement(6098..6754)
                     /locus_tag="PKX82_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HOF51401.1"
                     /translation="MTSPLPLDFSFVQLTVEFLATGAFALSGLLEAARKKLDAVGVVV
                     VAGLAAFGGGTMRDILLDRRPFFWMEHASWLWALLALCVLAMLFLRARHFEVTERAML
                     WPDAIGLGLFSASGAQIALDMQMPAIVAVMMGVITAVFGGVLRDIVCNEIPSAFRDHR
                     PYAICAFIGTWILVAAPWMGMPAWAALIASAATATLLRGIALISGYQLPAWNPGPRQE
                     "
     gene            complement(6874..>7506)
                     /locus_tag="PKX82_03790"
     CDS             complement(6874..>7506)
                     /locus_tag="PKX82_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HOF51402.1"
                     /translation="GPRQDDANGNAAPEDQQAQLGFVDADGQAAAAATPVDGNAAPAE
                     NESGEGREKRSRDRYGRERGPRQDRGERGERQQAPAEQAELAPVEAANAEAAPQAVAT
                     PVAAPVAVSKAAATAPAPAATGMPKVTTYALPLQSLQEVAQGSGLQWVNSDPERVATV
                     QAAIAAEPVAVHVPRERPAPVALDDRPLVLVETKRDLRNMTLPFEQEPAA"
BASE COUNT         1284 a         2280 c         2526 g         1416 t
ORIGIN      
        1 ccactggccc atgcgcagaa agctgtgcac cgggtggttg cccagctttt cttccaggtc
       61 ggcatagagc ttgggaatct cgcgcaggaa ggtggattcg tagtagctca gcgcattctc
      121 gatttcgtcg gccaccgtga gtttggaaaa acgcagcagg cgggtttgcc acagctgcat
      181 gacgcgggcc cgcatatggc cctcgttggc ggccagctcc cgtggcgata gggcatcttt
      241 cttgccggta ttcatggcgg cgcgcagctt gagttcgtcg cgtgcggtca acagctgggc
      301 aatgtcgcgc tcggcatcca gaatgctctt gcgctgcact tcggtcgggt gggcggtgag
      361 caccggtgac acgtagctgt gcgccagcgt gttggaaatg gtcttgggcg agatgccggc
      421 ccagcgcagg cgcgacatgg ccacctcgat gctgccttcc tgcgtatcgc cagcccgttc
      481 gtggatttca cggcggcgga tgtggtggcg gtcttcggcc agattggcca ggtggctaaa
      541 gtaggtaaac gcgcgaatca cgctcaccgt ctggtcgcca ctcaggccct tgaggagttt
      601 cttcagtgcc ttgtcggcct cgtggtcagc atcgcggcgg aatgccaccg agagcttgcg
      661 gatctgttcg atcagctcaa acgcctccac gccttcctgt tcacggatca catcgcccag
      721 aatgcgcccc aacaggcgga tgtcttccac cagtggacgt tcgttgtctt tgctgcgttt
      781 ggcgggggtg gcgaccggtg tcggtgaggg ggcggttttc atggtgacca tgctagcatc
      841 cgtgattgct ttttgtttac aaataggtta cgactgtgca acatttcacc atcgccacgc
      901 gcgaaagccg cctggccctc tggcaggccg aacatgtcaa ggcgcttctg gagcagcacg
      961 ggcacaaggt ctccctgctg ggtatgacca cccagggcga ccagatcctg gaccgcgccc
     1021 tgagcaaggt gggcggaaag ggcttgttcg tgaaggagct ggaagtggct ctggaagaag
     1081 ggcgtgccga tctggcggtc cattcgctca aggatgtacc catggaattg ccggccggat
     1141 tcaccctggc ctgcgtcatg gagcgcgaag acccgcgcga cgcctgggtg tcgtccaagt
     1201 acgccacgct gctggatttg ccacagggtg ccgtggtcgg gacctccagc ctgcgccgca
     1261 tggccttggt gcgggccctg cgcccggacc tcaagatcga ggccctgcgc ggcaatctgg
     1321 acacccggtt gcgcaagctc gacgacggcc tgtacgacgg cattgtgctg gcagcagccg
     1381 gtctcaagcg ccttggtttg cagtcccgca tccgcgccat cttcgagcca cagcagatgc
     1441 tgccggcggc cgggcagggc gctttgggta ttgaagtgcg tagtgaccgc gccgacgtga
     1501 ttgccgccct ggcgccgctg atgcaccagt ccacttggct ggcggtgtcg gctgagcgcg
     1561 cggtaagccg tgtcatgggt ggcagctgtt ccatgccact ggccgcgcat gcagtgttct
     1621 ccggtgacgt tttgaaaatc aacgcggctt ggggcgaccc tgatggccaa atgcccctgg
     1681 tgcagaccca ggcactggcg tcggtgagcg acaccgccgc tgctgtgcgt ctgggcgagc
     1741 aggtggccgc ggcgttgcaa gccggcgtgg aacaagcgac cggaaaagca cactgaccat
     1801 gcgcgtcatc ctgactcgac ccgagcgcga ggcgcgccaa tgggtcggtg cgctggcgca
     1861 ggcgggtatg gacgttattt ccatgccgct gattgcgatt gacggtccgc ccgacccacc
     1921 ggcggtggtc cgggcctggc agggtgtgga gcagctggat gcggtgatgt ttgtcagcgg
     1981 caacgcggta gaccactttt ttgcattaaa accgcctctg gcccccgtgt tcgttgcgca
     2041 gtcagctatc aaaacaagag cattcgtgac cggacctggc agccgggaag cactgctgcg
     2101 caacggcgtg gacgtggcgt gcatcgacgc gcccgatgcc caggccgggc agtttgattc
     2161 cgaggccctg tgggcggtgg tgcaacagcg ggtccacccc ggttttcggg tgctgatcgt
     2221 gcgtggtgcg gattccgccg atgcccgcag tgaaggcgcg gggcgcgact ggtttacccg
     2281 gcaggtcaag gcagcaggtg gccaggtgga gctggtggct tcctaccagc ggaggtgccc
     2341 gacgttgacc cctgctgaac tggcaacggc caggggcgcg gccacggatg gttcggtatg
     2401 gctgttcagc agttcggaag cggtggccaa cctgaactcc ctgctgcccg accagtcctg
     2461 gcagcaggcc cgtgcggtgg caacgcatcc gcgcattgtg caagcggcgc acatcgcagg
     2521 ttttggtgtt gtttgcgagt cacgtcccgt gctgggcgca gtggtcgcct cgatagaatc
     2581 gctgcaatga actccgaacc ggctgcacct tccgacaccc cttctgctgc atccgtgcct
     2641 atgccagagg tggcgctgca gtctgaaccg gtcaaagcgt cttcacgctg gttgtgggcg
     2701 gtggggctgg tgacagcggg tggcctgctg ggcagtgtga tgacctggca gaagctgggc
     2761 aatatccagg agcaactggc ccgccagagc gcggacaccg gagcgcaggc caccgaagcc
     2821 cgtgccaacg caaaacaggc ccaggaattg gccatggaga cggccgccaa gctggcggtg
     2881 acggacgccc gcgtgagcga ggtttccctg caacgtaccc aacttgaaga gctgatgcaa
     2941 agcctctcgc gctcgcgcga cgagaacatg gtggtcgata tcgagtcggc tgtgcgtctg
     3001 gcgcagcagc aggctcagct caccggcagc gtggagccct tgctggctgc gctcaagacc
     3061 gcagaccagc gcattgcccg tgcggcccag ccgcgcctga cacccctgca gcgcgcgatt
     3121 gcgaaagacg ttgcccgaat caagggtacg gccttggcgg acacgccggc gctgctggtc
     3181 aagctcgatg agctggtgct gcttgcggac gaactgcccg tgggtaatgc cgtaggggcc
     3241 acccggacgg cgccacagtt gcagcgcaag cctgaggaga ctgtgggctc gtggtggata
     3301 cgcatgggtc tgctggtgcg tgacgaactg cgcagcctgg tgcgcgtcag ccgcattggg
     3361 gaccccgatg ccgcactgct ttcgccggag caatcgttct tcgtacggga gaatctcaag
     3421 ctcaagctgc tcaatgccaa gctggggctg ttatcccgcc agattgactc tgcgcgtgcc
     3481 gatctggccg ctgcgggtgc ctcgatgggg cgttactttg atgccaactc ccgcaagacg
     3541 cagactgctg ccgttctgct ccagcaggtt cagagccaga tgcgcgcact ggaggtgccg
     3601 cgcgtggatg aaacccttgc agcgttgagc actgctgcgg cggggcgctg accatgcgct
     3661 ttgcactgtg gctcatggca ctgtttgcgg tggcaaccgc cagtgccctg tttgcgggca
     3721 ataaccagag caccgtgacg cttttctggg cgccttaccg ggtggacctg tcgctgaacc
     3781 tggtgctgct gatcctgctg gccatgttcg ttgtcttgca tctggccctg cgcgcgatca
     3841 atgccacgct gcgcctaccg cagcaggccc ggcgctggcg cttgcagcaa aaggagcggg
     3901 cgatttattc cgcgttgctc gattccctgt cgcacctggt ggcgggccgc tttatccggg
     3961 cgcgcaagtc ggcggagctg gtggtgtcgc tggaagactc ggtcaccaga ggcggcgagt
     4021 ccctggtcca cgtcgggaga ctgcgagcca tcacccattt gctggccgcc gagagcgccc
     4081 atgcgctgca ggacaagccg gtgcgcgatt cccacttcca gcaggccctg cagcacgccg
     4141 cacagcgcga tgtgcaggat gtgcgtgacg gtttgcacct gcgtgctgcg aggtgggcgt
     4201 ttgacgaacg ggatgctacg gctgccctgg aatggctgga tcagttgccc cagggggcct
     4261 cccgtcgcac attggcgctg cgcctgcgct tcaaggcggc gcgcctggcc ggacagaacc
     4321 gggaagcgct ggaggtggtc cgcctgctca ccaagcaccg ggccttttcc gaaggtgcag
     4381 gccgcagtat tgcccgcggc ctggccattg aactggtacg ttcagcccac gatccagtgc
     4441 agctcacacg ggcctgggac gcgctggaca ctgcggagcg tctgatgccg gacgtcgcca
     4501 tcgaggcgac tgagcgcttg ctgcaactgg ggggcgaagc gccgacggcg cggcaatggt
     4561 tgctaccggt atggagtgcc atgacaggca gttcggatgc gttggcgctg gtgcagcgtg
     4621 tccggctggt gcgggtgctg gaaaagagtt ttggcaaggg ggatggcgcg ccggacacca
     4681 cctggctcgg gcgcattgaa tcggcgcaaa tggccaaccc gcgggacgcc gcgctccagt
     4741 acctggccgg tgtggtctgc atgcgcttgc agttgtgggg caaagcacag caactgctca
     4801 aacagtcgct ttccatgctg caggacaacg gactcaaacg ggacgcttgg cgtgcgctgg
     4861 ccgaaatggc cgagcagcgg caggacatgc aggcagccac cgaagcctac cgcgaggcgc
     4921 tgaaactgcc ggtctgacac cgcgcgaagc ggggcaatcc cgagggtgac ggatcggctt
     4981 ttcagggcta ttatgtgccc atgcctacgc tcatcgaaca cctgatcaag ctcaccgacc
     5041 atcgggatcg cgacctgctg gaactgaccc tttccaaagc cctgattggc ctgctgccca
     5101 ttgaacgggt ggtgatggtc cgcgtggtca gcgaagaggg tgtcaggcga tggctggaaa
     5161 ttgcgtctct ggatgcgcgt gggggcggaa aagtggtgga cccgctgcgg gtggatttca
     5221 atgtgctgcg accggtcgag gacgacaagg accggttgca ctgcgtgcag tcccgcagca
     5281 atgtggaagt ggcctgggcg ggcgaggatg ggccgcgcat tacctacctg ccgctgttca
     5341 acgattcccg caacgacgac gaaggcgtgg tcgaggtgca ttccggttcg ccactcacga
     5401 atgagcagtt cctgaccatc ggcgcggtgc tgcatgtgtt tcgcaacatg tacaacttgc
     5461 tggcctacag cgaccgcgat gcacttacgg gcctgctcaa ccgcaagtct ctggacgaca
     5521 cgttttacag cgccgtgctg gaggaactgg aggaaggcaa ggcctcacgc atgcaggaac
     5581 tggccgcacc catcgagccc ggcaaggagc gtcgccactg cgtgccaccc aattactggc
     5641 tcggcactgt cagcgtggac cacttcgatg tgctgggcaa caagaacgga cacctgattg
     5701 ccgaagaggt gctgttgctg gtggcccgca tcatgaacaa caccttccgt acctacgacc
     5761 gcatctaccg ctttggcggc gagcagtttg cggtgctgat gcattgcccg gacgaggcgc
     5821 tggtgctggc agcctttgag cgcttccgcg ccaacatgga gcgcttcaac tttccgcagg
     5881 tgggtcgtgt tacggccagc gcgggtttca cccgcatctc tgccgacgat tcccccagca
     5941 ccgcgctgga gcgtgccgaa cgggctgtgg agtacgcgca gcaccacgga cacaacaagg
     6001 tgttcagcca tggcgacctg gtgcgcaagg ggctgctgga cggtgcgacc agaacgggtg
     6061 cggtcgacct gttctgagtc ttccagagcc ctgcttttta ctcctggcgc ggcccggggt
     6121 tccaggctgg caactggtag ccactgatta aagcaatgcc gcgcaggagt gtggcggtgg
     6181 cggctgaggc aatcagcgcg gcccatgcag gcatacccat ccagggtgcc gcaaccagaa
     6241 tccaggttcc gataaacgca caaatggcgt aggggcggtg gtcccggaag gcgctgggga
     6301 tttcattgca cacgatgtca cgcaacacgc cgccaaatac ggctgtgata acccccatca
     6361 tgacggccac aatcgccggc atctgcatgt ccagggcaat ctgcgcaccg gaggccgaaa
     6421 acagccccag tccaatggcg tcaggccaca gcatggcgcg ctctgtcacc tcaaagtggc
     6481 gtgcacgcag gaacagcatg gcgaggacgc acagggccag cagcgcccac agccacgagg
     6541 cgtgctccat ccagaagaag ggtctgcggt caagcaggat gtcccgcatg gtgccaccac
     6601 cgaatgcggc cagccctgcc accacgacta cacccaccgc atcgagtttc ttgcgcgcag
     6661 cctccagcag tccggacagc gcaaaggcgc ccgtggccag gaattccacc gtgagttgta
     6721 cgaaggaaaa gtccagagga agcggactgg tcatgtgatt ctccgggacg tgcgaaagtg
     6781 agtgtatttc cgggtcacgg attgtgggtc aaaaaaaggg gcacatccgt gcccctttcg
     6841 cttgctgcat gccctgttgt caggggaagc gcattacgct gcaggttcct gctcaaacgg
     6901 cagggtcatg ttgcgcaggt cgcgtttggt ttccaccagc accagcgggc ggtcgtccag
     6961 cgccacggga gcagggcgtt cgcgaggcac gtgcactgcg acaggttctg ccgcgatggc
     7021 tgcctgcacg gtggccacgc gctcgggatc gctgttgacc cactgcagac cggagccttg
     7081 tgccacttcc tgcagggatt gcaggggcaa cgcgtaggta gtgaccttgg gcatacccgt
     7141 ggccgcaggt gcaggtgcgg tcgctgctgc tttggacact gcaaccgggg ctgcgacagg
     7201 tgtcgccaca gcttgtggtg ctgcctcggc gttggcggct tctacaggcg ccagttctgc
     7261 ctgttcggca ggagcctgtt ggcgctcgcc gcgctcaccc cggtcctggc gtgggccacg
     7321 ctcgcggcca tagcggtcgc gggagcgctt ctcacggcct tcgccgcttt cgttttcagc
     7381 cggcgctgca ttgccatcca caggtgttgc tgcggctgct gcctggccat cggcgtccac
     7441 aaagcccaac tgggcttgct ggtcttctgg tgcggcgttg ccgttggcgt catcctggcg
     7501 tgggcc
//
LOCUS       DAOSJM010000070         7486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9050_length_7486_cov_4.508545, whole genome shotgun sequence.
ACCESSION   DAOSJM010000070 DAOSJM010000000
VERSION     DAOSJM010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7486
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9050_length_7486_cov_4.508545"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(222..875)
                     /locus_tag="PKX82_03795"
     CDS             complement(222..875)
                     /locus_tag="PKX82_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOF51403.1"
                     /translation="MKRLLPQRWWGVLKREWLLYHLGDPSYRFMFDAPPENEWVSLDC
                     ETTGLNTRTDEIISIGAVRIVGNRIMTSERLELLVRPERGVSAESVKIHRLRERDVAQ
                     GLPVEEAMKRLMHFIGSRPLVGYYLEFDLAMLHRAIWPMLGQGLPQPKIEVSALYYDY
                     KFKQLPAYQQQGNVDIDLRFATLMKDLNLPTRDAHDAVNDAVMAALAFIKLRTLRGQ"
     gene            complement(872..2698)
                     /locus_tag="PKX82_03800"
     CDS             complement(872..2698)
                     /locus_tag="PKX82_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOF51404.1"
                     /translation="MPSAFNFSASPFDCLTTDEQRLVRDSIDVVYFPEGETILDMGVA
                     PTHLFVIIKGFVTQFEGNEVVTTYGPDDAFDGRGLVAGKVSSRFMAAQEVVAYQLAKQ
                     AVSDLIAANATFGALLFSDLSNKLSALSARQNQHELQSLTMSRVDEAFLRPVHYVEVS
                     TDILTVVRIFQQHRTSSVLVRDDASSPPRLGIFTATALQRAILDGRPLNQLAVGGLSN
                     FSLIEVQASDQLGDAMAVMLKHRVHRVVVVDGSKIVGILEALDLFSFLSNQSHLITEK
                     IEEAKDLDGLSLAAAQITRMIVLLHRSGSRVNLIAKLVQQLNARLFERAWQLIAPPEL
                     VANSCLFVMGSEGRGEQLLKTDQDNGLILRDGYDAPANLQVLCQQFSAALASFGYPPC
                     PGNIMVSNPMWCQRATEFGQMARQWLVLPDSDSLMNLAIFMDAHTVCGDAHLLQGVQA
                     SLAQFVTDNEAVLARFAAAIDAFGNTVGWWNRLLGLGDTDQRMNLKKEGIFPLVHGVR
                     SLALAHHVTETSTAERIAALVAMGKLPAEMGPDLLDSLHFFMGLKLKAGLAELDMEQP
                     VTGAIDIAKLSSLDRDLLKDTLGVVKRFKALLHQRFHLDAIG"
     gene            complement(2729..3367)
                     /locus_tag="PKX82_03805"
     CDS             complement(2729..3367)
                     /locus_tag="PKX82_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HOF51405.1"
                     /translation="MKHITFYLDFISPFAYLAFEELPKALMGLSYSVTYKPVLFAALL
                     KHHGQLGPAEIPAKRDWTYRHVQWLAHSKSVPLDMPAAHPFNPLGLLRLAVATDPNGQ
                     PNRYVCETLLRHVWAGGGDAADAGRLKAITRELAPGRDPADASVKSQLRAHVEEAIAQ
                     GVFGVPAFEVDGKVFWGLDGLPMLRAYIEADAWFEGPAWDAVKAVPVGQSRQ"
     gene            complement(3364..3552)
                     /locus_tag="PKX82_03810"
     CDS             complement(3364..3552)
                     /locus_tag="PKX82_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HOF51406.1"
                     /translation="MHENIPSPCVSVCRMDAKREFCEGCLRSIEEIRAWSTSTDVQKK
                     IIWSHITQRLQARHASTP"
     gene            complement(3608..4087)
                     /locus_tag="PKX82_03815"
     CDS             complement(3608..4087)
                     /locus_tag="PKX82_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOF51407.1"
                     /translation="MPEGVQRVAAALADKNHPHAPVMLADAARTAQQAADALGIEVGQ
                     IAKSIIFRRKADGMAVLVITSGDRRVDEKKVEALVGKIGRADAEFVKAKTGFSIGGVS
                     PVAHAEPPLTLVDRELFRFAEIWAAAGHPHGVFKLRPQDLESLAGAPVADVTQAPTL"
     gene            complement(4171..5082)
                     /locus_tag="PKX82_03820"
     CDS             complement(4171..5082)
                     /locus_tag="PKX82_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOF51408.1"
                     /translation="MNYPTRVKLVDVGPRDGLQNEKTQVPAAVKVELVHRLQDAGLKE
                     IEVTSFVSPKWVPQMADNAEVMAAIERRADVRYSVLTPNLQGFEAALQTRPDEVVVFG
                     AASEAFSQKNINCSIAESIERFRPVVQAALEAGIAVRGAMSCTVGCPYEGEVAPERVA
                     YLAGLLKGIGVQRVDVADTIGVGTPRKVQRAIDAALQHYDIDSVSGHFHDTYGQALSN
                     TLAAMELGVWNFQSSVAGLGGCPYAKGATGNVSTEDVVYLLHGLGIDAGIDLDKLVDA
                     GKFISDFLGRKPNSRVATALLNKRQQT"
     gene            5158..5523
                     /locus_tag="PKX82_03825"
     CDS             5158..5523
                     /locus_tag="PKX82_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51409.1"
                     /translation="MKLSQIIPVSGIVAGTLLPCMAWTGPAADLGYPQVMDLLTETQG
                     NPAQVRPKLVGKTVGATLVAGKNSLWVVSPDDGVYFACAASAHGFKGGPVVAKVIQYE
                     APDNGEGGEPHLTLDRCSN"
     gene            complement(5553..6473)
                     /locus_tag="PKX82_03830"
     CDS             complement(5553..6473)
                     /locus_tag="PKX82_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HOF51410.1"
                     /translation="MRITFCCTDTAPEPWLAGLRAALPEANISLWEPGAPQADYAVVW
                     APPQQFFDEQTQLRALFNIGAGVDALLKMRLPAGAQVIRLDDAGMSVQMAEYVCHAVI
                     RHFREFAGYEADMANGKWSFRKPHSRLDYPVGVLGLGVLGERVARAVAQFDFPVNGWS
                     RTPKDVPGVQCFAGPAQLDAFLQASRMLVCLLPLTTETRDILNRDSLSRLQPGGYVIN
                     VARGAHLVDEDLLALLDSGHLAGATLDVFRVEPLPRSHPFWNHPKISLTPHTSARTLR
                     TESIAQIAFKIGALQRGESVLGCVDAARGY"
     gene            complement(6497..7486)
                     /locus_tag="PKX82_03835"
                     /pseudo
     CDS             complement(6497..7486)
                     /locus_tag="PKX82_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794380.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
BASE COUNT         1411 a         2456 c         2246 g         1373 t
ORIGIN      
        1 ccgcgctgtt ctccatggcg atcggcttta tcggcgtgtg gttgttctcg gtgctggacc
       61 gtagccccaa cgcggccaaa gagcgcgctg catttcctgc gcagcaagtg cgttcggaaa
      121 ccggtctggg tgcctctggc gcatcgggcc actaagccca caacgctgcg ggcacctggc
      181 ccgcatgctt tgccatccaa ccgggcctcg cgcccggttt tttattgccc gcgtaacgtt
      241 ctcagcttga tgaatgccaa cgccgccatg accgcatcgt tcaccgcgtc atgggcatcg
      301 cgcgtgggca gattcaggtc tttcatcagg gtggcaaagc gcaggtcaat atcgacattg
      361 ccttgctgct ggtaggcggg cagctgtttg aacttgtagt cgtagtacag cgcggagact
      421 tcgattttcg gctgcggcaa cccctgcccc agcatgggcc aaatggcccg gtgcaacatg
      481 gccaggtcaa actccaggta gtagcccacc aaagggcggc tgccaatgaa atgcatgagg
      541 cgcttcatgg cctcctccac cggcaggccc tgggccacat cgcgttcgcg tagccggtga
      601 atcttcacgc tctcggccga cacgccgcgc tcgggacgta ccagcagttc gagccgttca
      661 ctggtcatga tgcggttacc cacaatccgc accgcaccta tggaaatgat ttcgtcggtg
      721 cgggtgttca gcccggtagt ctcgcaatcc agcgacaccc attcattctc gggcggcgca
      781 tcgaacatga accggtagct tgggtcgccc aggtggtaca gcagccactc cctcttgagc
      841 acaccccacc accgctgcgg cagcaggcgc ttcatccgat cgcatccaga tgaaatcgct
      901 ggtgcagcaa ggccttgaag cgcttgacca cccccagtgt gtccttgagc aggtcgcggt
      961 ccagactgct caatttggct atatcaatcg cgcccgtcac tggttgctcc atgtcgagct
     1021 cggccagtcc cgccttgagt ttgaggccca tgaaaaaatg caggctgtcc agcaggtcgg
     1081 gccccatctc ggcgggcagt ttgcccatgg ccaccagcgc ggcgatgcgc tcggccgtgc
     1141 tggtctcggt cacatggtgg gccagcgcca gactgcgcac accgtgcacc agcgggaaga
     1201 tgccctcttt cttcagattc atccgctggt cagtatcgcc cagccccagc agtcggttcc
     1261 accagcctac agtgttgcca aaggcatcaa tggctgccgc aaagcgtgcc agcaccgcct
     1321 cgttgtcggt gacaaattgc gccagactgg cctgcacgcc ctgcaacagg tgtgcatcgc
     1381 cacagaccgt gtgcgcatcc atgaagatgg ccaggttcat caggctgtcg ctgtcgggca
     1441 agaccagcca ctggcgcgcc atctggccga actcggtcgc acgctggcac cacatcgggt
     1501 tgctcaccat gatgtttccg gggcaaggtg ggtagccaaa actggcgagc gctgccgaga
     1561 attgctggca caagacctgc aggttcgcgg gtgcgtcata gccatcgcgc aagatgagtc
     1621 cgttgtcctg gtcggtcttg agcaactgtt ctccgcgccc ttcgctgccc atcacaaaca
     1681 ggcagctgtt agccaccagc tccggtggtg caatcaattg ccaggcacgc tcgaacaggc
     1741 gggcattgag ctgttgcacc agcttggcaa tcagattgac gcgcgaaccg ctgcggtgca
     1801 acagcacaat catgcgagtg atctgcgccg cagcaagact cagcccatcc aggtctttgg
     1861 cctcttcgat cttttcggta atcaggtgcg actggttgga cagaaagctg aacaggtcaa
     1921 gtgcctccag aatgcccaca attttgctgc catccaccac caccacgcgg tgcacgcggt
     1981 gcttgagcat caccgccatg gcgtcaccca actggtccga tgcctgcacc tcgatcagcg
     2041 aaaagttcga cagcccaccc accgccagct ggttcagcgg acggccatcc agaatggcac
     2101 gctgcaacgc tgtggcggta aaaataccca gccgcggtgg cgaacttgca tcatcgcgaa
     2161 ccagcacgct ggaggtgcgg tgctgctgaa aaatacggac gaccgtcaga atatcggtac
     2221 tgacctccac atagtgcacc ggacgcagaa aagcctcgtc cacgcgggac atggtgagcg
     2281 actgcagttc atgctggttc tggcgtgcag acagcgcact gagcttattg ctcagatcag
     2341 aaaacaacaa ggcaccaaac gtggcattgg cagcaatcag gtcactgacc gcctgcttgg
     2401 ccaactggta ggccaccacc tcctgggccg ccataaagcg gctgctgacc ttgccagcca
     2461 ccagcccccg gccatcgaag gcatcgtccg gcccgtaggt ggtaacgacc tcgttgccct
     2521 cgaactgggt gacaaagccc ttgatgatca caaacagatg ggtgggcgcg acacccatgt
     2581 caagaatggt ctcgccttcc gggaaataca ccacatcaat actgtcgcgc accaggcgct
     2641 gctcatcggt cgtcaggcag tcaaacggtg aagcagaaaa attgaaggca ctgggcatgg
     2701 tgggattttc cacctccacc ctgcgacttt actgacggga ctggcccacc ggcacggcct
     2761 tcactgcgtc ccaggcgggc ccctcaaacc aggcgtctgc ctcgatataa gcccgcagca
     2821 tgggcaagcc atccagcccc cagaacacct tgccgtccac ctcgaatgcg gggacgccaa
     2881 atacgccttg ggcaatggct tcctccacat gggcccgcag ttgcgatttg accgatgcgt
     2941 ccgcggggtc acgtccaggt gccagctcgc gggtgatggc tttcagacgg cccgcgtcgg
     3001 cagcatcgcc accacccgcc cacacatggc gcagcagggt ttcgcacaca tagcggttgg
     3061 gttgcccgtt cgggtccgtg gcgaccgcca ggcgcaacag ccccagcgga ttgaagggat
     3121 gggccgccgg catgtccagc ggcacactct tgctgtgggc cagccattgc acatggcggt
     3181 aggtccagtc gcgcttggcc ggaatttcag cggggcccag ttgcccatgg tgtttgagca
     3241 gggcggcaaa cagcaccggt ttgtaagtga ccgaatagct cagccccatc aacgccttgg
     3301 gcaactcctc aaacgccagg taggcaaagg gagaaatgaa gtcgaggtaa aaggtgatgt
     3361 gcttcatggc gtgctggcgt gccgtgcctg caggcgttgc gtgatgtgcg accagatgat
     3421 cttcttctgc acatcggtgc tggtactcca ggcccggatt tcttcgatgc tgcgcaggca
     3481 gccctcgcaa aattcccgct ttgcatccat gcgacacacg gatacgcaag gagaaggaat
     3541 attttcgtgc atagcccccg ccgttattgc gcggtcagct agcattttca tagcattcat
     3601 acaatcctta cagcgtgggc gcctgggtca catcggccac cggcgcacca gccagcgact
     3661 ccagatcctg cggccgcagc ttgaacaccc catgcgggtg cccggcagcg gcccagattt
     3721 cggcaaagcg aaacagctcg cgatccacca gcgtcagcgg tggttctgca tgggcgaccg
     3781 gcgacacacc gccaatgctg aatccggttt tggccttgac gaactcggca tcggcacggc
     3841 caatcttgcc gaccagggct tctacttttt tctcgtccac ccgccggtcg cccgaggtga
     3901 tgaccagcac cgccataccg tcggccttgc gccggaagat gatgctcttg gcaatctggc
     3961 ccacttcaat ccccagtgca tcggccgcct gctgggccgt gcgggcggca tcggccagca
     4021 tgacgggcgc atgtgggtga ttcttgtccg ccagggcggc cgccacgcgt tgcacgccct
     4081 cgggcagact atcgccccca cgctccccca caacgtgtgg gtcgctaccc cccaaggggg
     4141 ccctcgcgcc ttggggctgc ccggcggcgc tcatgtctgc tgccgcttgt taagcaaggc
     4201 ggtggccacc cgcgagttgg gcttgcggcc caggaagtcg ctgatgaact tgccggcgtc
     4261 caccagcttg tccaggtcaa tgccggcgtc aatgcccagc ccatggagca ggtacaccac
     4321 gtcttccgtg gagacgttgc cggtagcgcc cttggcatag gggcagccgc ccaggccggc
     4381 cacggaggac tggaagttcc acacccccag ctccatcgcc gccagggtgt tggacagcgc
     4441 ctgaccgtag gtgtcgtgga agtgaccaga gaccgaatcg atgtcgtaat gctggagcgc
     4501 cgcgtcgatg gcgcgctgca ccttgcgggg cgtgcccacg ccgatggtgt cggccacgtc
     4561 cacccgctgc acgccgatgc ccttgagcag gccagccagg taggccacac gctcgggcgc
     4621 cacctcgccc tcgtaggggc agcccacggt gcagctcatg gcgccgcgca cggcgatgcc
     4681 agcctccagc gccgcctgca ccactgggcg gaaacgctcg atactctcgg caatgctgca
     4741 gttgatgttt ttctggctga aggcctcact ggccgcgcca aacaccacta cctcatccgg
     4801 gcgggtttgg agtgcagcct cgaagccctg caggttgggc gtcagcaccg aatagcgcac
     4861 atctgcgcgg cgctcgatgg cggccatgac ttccgcgttg tccgccatct gtggcaccca
     4921 tttgggcgac acgaagctgg tgacctcaat ctccttcagg ccagcatcct gcaaccggtg
     4981 caccagctcc accttgacgg ccgcgggcac ctgggtcttt tcattctgca ggccgtcgcg
     5041 gggaccaaca tcgaccagtt tgacgcgtgt ggggtagttc atacagggct tgcgaatttc
     5101 aggtttcgct ctctagaggg aaatagggca atctcaagtt ttacgcagtg aagtgccatg
     5161 aaactgagtc aaattattcc ggttagcggt attgtcgccg gtacgctgct gccatgcatg
     5221 gcatggacag gcccggcagc cgatctgggc tacccccagg tgatggacct gctgacggaa
     5281 acccagggca acccggcgca ggttcgtccc aaactggtgg ggaaaaccgt cggcgccacc
     5341 ttggtggcgg gtaaaaactc gctttgggtc gtgtcgccgg acgacggtgt gtacttcgcc
     5401 tgcgctgcca gcgcccacgg cttcaagggt ggccccgtag tggccaaagt tatccagtac
     5461 gaagcgccgg acaacggcga agggggtgag ccccacctca cgctggaccg ctgcagcaac
     5521 tgaagtcacg cacaccaccg ggtcacccgc gcctaatagc cacgcgccgc atccacgcag
     5581 cccaaaacgg actcaccccg ttgcagggcg ccgatcttga aggcaatctg cgcaatgctc
     5641 tcggtgcgca acgtgcgcgc cgaggtgtgc ggggtgagag aaatcttggg gtggttccag
     5701 aacggatggc ttcggggcag cggctccacg cgaaacacat cgagtgtggc acccgccaga
     5761 tggccactgt ccagcagcgc cagcaaatcc tcatccacca ggtgcgcacc ccgtgccaca
     5821 ttgatcacat agccaccagg ttgcaggcga gagagactgt cgcggttcag gatgtcccgc
     5881 gtctctgtgg tcagcggcag caggcacacc agcatgcggc tggcctgtag aaaagcgtcc
     5941 agctgcgccg gcccggcaaa gcattgcacg ccgggtacat ccttgggcgt acggctccag
     6001 ccattgaccg gaaaatcaaa ctgcgccacc gcccgggcca cgcgctcacc gagcaccccc
     6061 agccccagca caccgacggg atagtccagc cggctgtgcg gcttgcgaaa cgaccacttg
     6121 ccgttcgcca tatcggcttc atacccggca aactcgcgaa agtggcggat caccgcatgg
     6181 cacacatact cggccatctg cacggacatg ccggcgtcgt ccaggcggat gacctgtgca
     6241 ccagcaggca ggcgcatttt cagcaaggcg tccaccccgg caccgatatt gaacagggca
     6301 cgcagctggg tttgttcgtc aaagaactgc tggggcggcg cccacaccac ggcgtagtct
     6361 gcctgcggcg caccgggctc ccacagggaa atgtttgcct ccggcaatgc ggcgcgcagc
     6421 ccggccagcc agggctccgg agccgtatcg gtgcaacaga acgtaatgcg cacaggccgc
     6481 tctatctgtc tgtatgtcag gctttggcca cttccatgcg cagcagctcc gaaccttcgg
     6541 tcacctggtc accgggagca aacagcagtt cggccacggt gccatcggcg ggcgctgcaa
     6601 tggtgtgctc catcttcatg gcttccatca ccgccagcgc ctggcccttt ttgaccgcat
     6661 cacccgcctt gaccgcaaac gacaccacct tgcctggcat gggcgcggtc agacggccgc
     6721 cttcgagctg gccatccccc gcatgtgcca gcgcatcaat tgctatgatt tgtgtagcgc
     6781 cttgcgcagc aaatacgtgg gccacatccc cttttttgta cacatggcag ctggcgcgct
     6841 gcgcgcccca gccacatcga tggcgccggt ggcctgcgcg ctgtagcgca gcacggcctc
     6901 ttcaccggac acctgcagca gcacgtcgcc atcgtgcacc gcggtgagtt gcactgtctg
     6961 cggctgcccc tgaaactcca tggcaaagct gcgcacgctg gggccgtggg agcgccagtt
     7021 gtcgcgctgc gcccaggggt cgatccagcc actggtgtcg cgccgtgcgg cgatggcctg
     7081 ctcgcccagc agggtgtgcg acaccagcgc agcagcggcc aggcccagcc cgactttttc
     7141 ctgcttgaac agcaccgcgg cctcgcgcgg aatcagggcg gtatccagat tggcctgtgc
     7201 gaacgacgca ctggtggtga cgtggcgcag gaactgcacg ttggtggcca gtccgacgat
     7261 atgggtctgc gccagggcag cgtccagtcg ggccagtgct tccgcacgtg tcgcgccgtg
     7321 cacgatcagc ttggccacca tggagtcgta atacgggctg atggtgtcgc cctcgcgcac
     7381 accggcatcc acgcgcacgc cgttgtcggc acgctcgaac gtggtgcagg cgggtaggcc
     7441 atacacgtgc agtgtgccgg tggccggcag gaagttgttg tcgggg
//
LOCUS       DAOSJM010000071         7412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9164_length_7412_cov_4.541661, whole genome shotgun sequence.
ACCESSION   DAOSJM010000071 DAOSJM010000000
VERSION     DAOSJM010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9164_length_7412_cov_4.541661"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKX82_03840"
     CDS             complement(<1..595)
                     /locus_tag="PKX82_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOF51411.1"
                     /translation="MTTAAGFSLKRQLLLWLLLPQLILWLMGGVLAFRVAMTYAEKAI
                     DQSLTQSVRSLARQVKPFGSGLLVDFPKAAQDILEQDPKDRVSYMVSSPPGSFLLGNS
                     KLPGPPADTTPTGNEPVLYNVELDGKTMRVATIDVSFGEGPTQQRMRVQVAKSLSVQE
                     SIARELVRDVLFPLLLLGAFMSFLVFAGIQRGLAPLQR"
     gene            complement(592..1293)
                     /locus_tag="PKX82_03845"
     CDS             complement(592..1293)
                     /locus_tag="PKX82_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF51412.1"
                     /translation="MSHLLIVEDDELLRDGLCAQLMRAGHNVSSAVDGAQAQSMLETT
                     RFDAVILDLGLPVIDGVAVLKWIRQRLAALPVLVLTARDGIEDRVQGLNAGADDYLTK
                     PFDMAELLARLQAMLRRSRMPAFGGTLEVQGAANKRLRLDPNLPLGWLDDEPMELTRR
                     EWSLLSLLVGNLGQVVGREDVLAVWQSEPTETGTGASNALEVYVHRLRRKLQDSGLSI
                     RNVRGLGYMLEMDPP"
     gene            complement(1327..2253)
                     /locus_tag="PKX82_03850"
     CDS             complement(1327..2253)
                     /locus_tag="PKX82_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF51413.1"
                     /translation="MNGCNINPIDIELTRDALATSTLNHGLKVLGDQWTVAILLGAFL
                     GWRRFDEWQGQLAIPRQTLSQRLKTLVDMGVLRQRPYQERPVRMAYHLTRHGLALYGP
                     VLMIWAWERRWGARAGALPQKLVHRSCGHRFRPVMACGHCGERAGPDTLTFTLAPNPE
                     LLPPAQDTARSARMASNTQPMGLGLRVDRWALLILSAVYLGCRYFDQLAQVLQIVPSV
                     LTRRLGNLVEDGLLLSQPDVQDGRRTVYRLTPASRDLFPYVMAFAHWADQHFGQAGSI
                     RPTHKACGMPFVPSIQCSACHSEVSPHDVTYT"
     gene            complement(2225..3439)
                     /locus_tag="PKX82_03855"
     CDS             complement(2225..3439)
                     /locus_tag="PKX82_03855"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOF51414.1"
                     /translation="MPHALIIDAVRSPRGRANARGALHPLRPVTLLAQQLQHLAQRTG
                     IDTAAVTDTLMGCVTQTGEQGTNIAKLAVVSAGWSDAVSALTLNRYCASGLTAVQFAA
                     QQALANDGLAVGGGLEMMSRVPMASDQGPLTHDTALQAQTRLIPIGLAADTIATLHGF
                     SRAQCDDYALASQERACHARAQGHFVSLTPALSEDGQVLLAQDETPRDNTTLDSLTRM
                     PSAFATLGAESGLDAMLCARYQLQNIQHVHHAGNSPAMADGASAVLVASPQAAARLGL
                     QARGRILAVADAGVNHVLALTGAVDATRKALARAGLSVADIDLFEVNESFAALMLHYM
                     QQLGVPHARLNTNGGTIALGHAMGATGGNLVGMAMDELERRHARYAVIAICGAAGVAV
                     AMVIERVQHQPH"
     gene            complement(3441..4685)
                     /locus_tag="PKX82_03860"
     CDS             complement(3441..4685)
                     /locus_tag="PKX82_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HOF51415.1"
                     /translation="MSAPVYILGGYQTDFVKAWSRHGQDISDMVRETTLQTLDACTLE
                     PQAIESIHVGNAFGELQRQQAHLGSMVAQVVPELWGVPAMRHEGACASSSLGILSAMA
                     EIEAGRYDCVLVLGAEEFKNLPGDQASQNQNSAAWQGHEDIDCKFMWPAVFGLLAQEY
                     DARYGLDRKYLNRIAELNYANARRNPKAQTRNWQFDALSFTDDDAANPVIEPGTRRQD
                     CGQITDGACAVVLASERFAKAHQARMGKSLSDLAHIAGWGHRNASLRARDKLERSRGQ
                     PYVFPHVRQTIEDAWRRAGVSGIDAMSGIETHDCFTTTQYMAIDHLGLTPAGQSWQAV
                     EDGSIERGGRCPVNMSGGLIGAGHPVGATGVRMLFDAARQVTGQADACQIEGANRIQT
                     LNIGGSCGTVVSFVVERAAQGH"
     gene            complement(4682..6142)
                     /locus_tag="PKX82_03865"
     CDS             complement(4682..6142)
                     /locus_tag="PKX82_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HOF51416.1"
                     /translation="MNSRANPNDLAPFGEDYGPTLMGVYAPTAGEHVLKDLPLLKGRI
                     PADLNGVYLRAGPNARYAPNGRYHPFDGDGMVHAAQFDKGTLTYRNRWVQTDGWKEED
                     QAGKSTFYGIRETLKDRPDKRLKDTANTDLVAHAGKALALWYMSGDAYEIDPVTLQTL
                     GKSQAIRESGGRISAHAKVDEITEDLMFFDYGNEAPYMHYGVVGADGKLKHYVPIDLP
                     GPRLPHDMAVTEHYSILHDLPLFHEEEALKLGRHKVAFYPDVPTRFGVMPRLGASDSI
                     RWFDFTPCFVYHVVNSWEDGDWVVMVGCRYMPALDAQGQIDLVRTARDVAELVMHARL
                     WVWRMNMATGATKEHVLDPDRNVEFPTFNSRLTGRRTRFGYLVDHSEKKTLQWYGIRK
                     YDLDTGESLGSWSDDPQNSWYSEPWFAAADNPQSEDHGYIISFQWNEALSRETLDIFD
                     ARDLSAGPVAQVQIPHRIPLGFHACWMAADRMKAAA"
     gene            complement(6260..>7412)
                     /locus_tag="PKX82_03870"
     CDS             complement(6260..>7412)
                     /locus_tag="PKX82_03870"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HOF51417.1"
                     /translation="LLDQGYVAVGQDFPVFLHPQSKEEYALARTERKTAPGYKGFAVH
                     YAPEVTLDEDLGRRDLTINSIAIHAEDMRADGTFDLKNLCDPYGGQQDIAARVLRHTT
                     EAFQEDPVRILRLARFAARFTDFTVAPETMALMRDMVDHGDVDHLVAERVWQELSRGL
                     MEAQPSRMFAVLRECGALAHLLPELDRLWGVPQSVEHHPEVDTGVHVMLVVDMAAQLH
                     APLEVRFAALTHDLGKGTTAPNMLPRHIGHEERSVRLLKGLCQRLRVPTDCAELAQVV
                     AREHGNIHRSQDLSATATVRLLERCDAFRKPTRFADILLACECDARGRLGLQDTPYPQ
                     GRRLAQALQTALGVATNAIAETAMAAGARGPKIGELIQAARAAAIAATP"
BASE COUNT         1239 a         2335 c         2474 g         1364 t
ORIGIN      
        1 gccgctgcag cggggccagg ccacgctgga tgccggcgaa gaccaggaaa ctcatgaacg
       61 cacccagcag cagcagcgga aacaacacgt cgcgcaccag ctcgcgcgca atgctttcct
      121 gtaccgacag gctcttggcc acctgcaccc gcatgcgttg ctgggttggc ccctcgccaa
      181 agctcacatc aatggtcgcc acccgcatgg tcttgccatc gagctcgacg ttgtagagca
      241 cgggctcgtt gccggtgggc gtggtgtctg caggcgggcc cggcagtttg ctgttgccca
      301 gtagaaagct gcccggtggc gacgacacca tgtaagacac gcggtccttg gggtcctgtt
      361 ccagaatgtc ctgtgcggcc ttgggaaaat ccaccagcag gccggaccca aagggtttga
      421 cctgccgggc cagcgagcgc accgattgcg tcagcgactg gtcgatggct ttttcggcat
      481 aggtcatggc cacacgaaag gccagtaccc cgcccatcag ccacaggatg agttgcggga
      541 gcaataacca cagcagcaac tggcgtttga gcgagaagcc tgccgccgtg gtcatggagg
      601 atccatctcc agcatgtagc ccaacccgcg cacgttgcgg atcgacagac ccgagtcctg
      661 cagcttgcgg cgcagccggt gcacatagac ctccagtgcg ttggaggcac cggtgccggt
      721 ctccgtgggc tcggattgcc atacggccag cacgtcttcg cgcccgacca cctggcccag
      781 gttgcccacc agcagggaca gcagcgacca ttcacggcgg gtcagttcca tgggctcgtc
      841 gtcgagccag cccaagggca ggttggggtc aagccgcagg cgtttgttag cagctccctg
      901 cacctccagt gtgccgccaa aggccggcat ccgggatcgg cgcagcatgg cttgcaggcg
      961 tgccagcaat tcggccatgt cgaagggttt ggtgaggtaa tcgtctgcac cagcgttgag
     1021 gccctgtacc cggtcctcaa tgccgtcgcg tgccgtcagc accagtaccg gcagggcggc
     1081 caggcgctgg cgaatccatt tgagtaccgc gacaccgtcg atgacgggca gccccaggtc
     1141 gagaatgacg gcgtcaaaac gcgtggtttc cagcatggac tgcgcttgtg caccgtccac
     1201 tgcgctgctc acgttgtgcc ctgcgcgcat cagttgtgcg cacaggccat cgcgcagcaa
     1261 ttcatcgtct tcaacaatca gcaaatggga catggcggga gcataccaag ttgccgcggc
     1321 gcaggctcag gtgtaagtca cgtcgtgggg cgacacctcg gagtggcacg cgctgcactg
     1381 gatgctgggg acaaagggca tgccacaggc cttgtgggta gggcggatcg agcccgcctg
     1441 cccgaaatgc tggtcggccc aatgcgcaaa ggccatgacg taggggaaca ggtcgcggct
     1501 ggcgggtgtc aggcggtaca ccgtacggcg gccgtcctgc acgtccggtt gggacagcag
     1561 cagaccgtcc tcgacgaggt tgcccaggcg gcgcgtcagt acgctgggaa ctatctgcag
     1621 cacctgcgcg agctggtcga agtagcggca tcccaggtag accgctgaaa ggatgagcaa
     1681 ggcccagcgg tccacgcgca ggcccaggcc catgggctgt gtgttcgatg ccatgcgcgc
     1741 gctgcgtgcc gtgtcctgcg ctggtggcag cagttccgga ttaggggcga gcgtgaacgt
     1801 cagcgtgtcg gggccggcac gttccccaca gtgcccgcag gccatgacgg gccgaaacct
     1861 gtgtccgcaa ctgcggtgca ccagcttctg gggcagtgcg ccagcgcgtg caccccagcg
     1921 gcgttcccac gcccaaatca tcagcaccgg gccatagagt gccaggccgt gtcgggtgag
     1981 gtggtatgcc atgcggaccg ggcgctcctg gtaggggcgc tggcgcagca cgcccatgtc
     2041 taccagggtc ttgagccgct gtgacagggt ctggcgcgga atggccagct gaccctgcca
     2101 ttcgtcaaaa cggcgccagc ccaggaatgc gcccagcagg atggccacgg tccactggtc
     2161 gcccagtacc ttgaggccgt ggttcaaggt gctggtggcc agcgcgtcgc gcgtgagttc
     2221 aatgtcaatg gggttgatgt tgcacccgtt caatcaccat ggccactgcc acgccggctg
     2281 cgccgcagat ggcaatcacc gcataccgcg catggcggcg ttccagttca tccatggcca
     2341 tgccgaccag gttgccgccc gtggcgccca tggcatggcc cagggcgatg gtaccgccgt
     2401 tggtgttgag cctggcgtgc ggcacgccca gttgctgcat gtagtgcagc atgagcgccg
     2461 caaacgattc gttgacctca aacaggtcta tgtccgctac cgacagcccc gcacgtgcca
     2521 gcgccttgcg cgtggcatct accgcgccgg tcagcgccag cacatggttc acgcccgcgt
     2581 ctgccacggc cagaatgcgc ccgcgggctt gcagacccag ccgcgcggcg gcttgtgggc
     2641 tggccacgag tacggcggag gcaccatcgg ccatggcagg cgaattgccg gcgtggtgga
     2701 catgctgaat attctgcagt tgatagcgtg cgcacagcat ggcatccaga ccactttctg
     2761 cgcccagcgt ggcaaatgcg ctgggcatgc gggtcagcga atccagggtg gtgttgtcgc
     2821 gcggtgtttc gtcctgcgcc agcagtacct ggccgtcttc ggacagggcc ggagtaagtg
     2881 acacaaagtg tccctgcgcg cgcgcatggc aggcgcgttc ctgcgaggcc agcgcgtagt
     2941 cgtcgcactg ggcgcggctg aatccgtgca gggtggcgat ggtgtcggct gccaagccaa
     3001 tcggaatcag gcgggtctgc gcctgcaatg cggtgtcgtg ggtcagtggg ccctggtccg
     3061 aggccatggg cacgcgcgac atcatctcca ggcctccacc caccgccaat ccgtcgttag
     3121 ccagcgcctg ttgcgcggcg aactgcacgg ccgtgaggcc cgaggcacag tagcggttga
     3181 gggtcagtgc ggagaccgca tcgctccatc cggcagacac caccgccagt ttggcaatgt
     3241 tggtgccctg ttcgccggtc tgggtgacac agcccatgag ggtgtcagtc accgccgcgg
     3301 tgtcgatgcc agtgcgctgt gccaagtgtt gcaactgctg tgccagcaag gtgacgggac
     3361 gcagcgggtg caaggcaccc cgcgcattgg cgcgtccgcg tgggctgcgc accgcgtcga
     3421 tgatcagggc atgtggcacg ttagtgtccc tgtgccgcgc gctcgaccac aaagctcacc
     3481 accgtaccgc aggagccacc gatgttgagc gtctggatgc ggttggcgcc ctcaatctgg
     3541 catgcgtccg cctgcccggt gacctggcgc gctgcatcga acagcatgcg cacgccggtg
     3601 gcgcctacgg ggtgaccagc accaatcagc ccgccactca tgttgacagg gcagcgccca
     3661 ccgcgctcga tgctgccgtc ttccaccgct tgccagctct ggcccgcagg cgtcaggccc
     3721 agatggtcaa tggccatgta ctgcgtggtg gtgaagcagt cgtgggtttc gatgccgctc
     3781 atggcgtcga tgcctgacac gccggcacgc ctccaggcat cctcgatggt ctggcgcaca
     3841 tgggggaata catagggctg cccccggctg cgctccagct tgtcgcgtgc gcgcaggctg
     3901 gcattgcggt gcccccagcc ggcgatatgg gccaggtcag acaaggactt gcccatgcgt
     3961 gcctggtgcg ctttcgcaaa gcgctccgag gccaagacca cggcgcaggc gccatcggtg
     4021 atctgaccgc agtcctggcg gcgggtgccc ggttcaatga ccgggttggc tgcgtcgtca
     4081 tcggtgaagc tcagggcgtc gaactgccag ttgcgcgtct gcgccttggg gttgcgccgg
     4141 gcattggcgt aattgagttc ggcaatgcgg ttgaggtatt tgcggtcgag tccataacgc
     4201 gcgtcgtatt cctgcgcgag caggccaaac accgcgggcc acatgaactt gcagtcaatg
     4261 tcctcatggc cctgccaggc ggccgagttc tggttttgcg aagcctggtc gcccggcagg
     4321 ttcttgaact cttcggcacc gagtaccagc acacagtcgt aacggccggc ctcgatctcg
     4381 gccatggccg agagaatgcc cagtgacgac gaggcacagg cgccttcgtg cctcatggca
     4441 ggcacgcccc agagctccgg caccacctgc gccaccatcg agcccaggtg ggcctgctgg
     4501 cgctgtaact cgccaaacgc gttgcccacg tggatgctct cgatcgcttg cggctccaac
     4561 gtgcaggcgt ccagcgtctg cagtgtggtt tcgcgcacca tgtcggagat gtcctgcccg
     4621 tggcgcgacc aggctttgac aaagtctgtc tggtagccgc ccaggatgta caccggtgcg
     4681 ctcatgcagc tgccttcatg cggtccgccg ccatccagca ggcgtggaag cccaggggga
     4741 tacggtgcgg aatctgcacc tgcgccaccg ggccggccga caggtcacgg gcatcaaaaa
     4801 tgtccagtgt ttcgcgtgac agggcctcgt tccactggaa gctgatgatg tagccgtggt
     4861 cttcagattg cgggttatcg gccgcggcaa accagggttc cgaataccag ctgttctgcg
     4921 gatcgtccga ccagcttccc aggctttcgc cggtgtcgag gtcgtacttg cggatgccgt
     4981 accactgcag ggtcttcttt tcggagtggt cgaccaggta gccaaagcgc gtgcgccgtc
     5041 cggtcaggcg gctgttgaag gtggggaact ctacgttgcg gtcggggtcg agcacatgct
     5101 ctttggtggc gccggttgcc atgttcatgc gccagaccca caagcgtgcg tgcatgacga
     5161 gttcggccac atcacgggcg gtacgcacca ggtcgatttg gccctgggcg tccagtgccg
     5221 gcatgtaccg gcaacccacc atgacgaccc agtcaccgtc ctcccaggag ttgaccacgt
     5281 ggtacacaaa gcagggtgtg aagtcaaacc agcggatcga gtccgacgcg cccagacgcg
     5341 gcatcacgcc aaagcgggtc ggtacgtcgg ggtagaacgc caccttgtgg cggcccagct
     5401 tcagcgcctc ttcttcatgg aatagcggca ggtcgtggag gatcgaatag tgctcggtca
     5461 ccgccatgtc atgcggcagg cgtggccccg gcaggtcgat cggcacatag tgcttgagct
     5521 ttccgtccgc gccaacgacc ccgtagtgca tgtacggggc ttcgttgccg tagtcgaaaa
     5581 acatcaggtc ttcggtgatt tcatcgacct tggcgtgggc cgagatgcgg ccgccgctct
     5641 cgcggatggc ctgggacttg cccagggtct gcagggtgac ggggtcgatc tcgtacgcat
     5701 cgcccgacat gtaccagagc gccagcgcct tgccggcgtg ggccaccagg tcggtattgg
     5761 cggtgtcctt caggcgcttg tccgggcggt ccttcagggt ctcgcggatg ccatagaaag
     5821 tgcttttacc cgcctggtct tcctctttcc agccatcggt ctgcacccag cggttgcggt
     5881 aggtgagtgt gcccttgtca aactgcgcgg cgtgcaccat gccgtcgcca tcgaatgggt
     5941 ggtatcggcc attgggggca tagcgcgcat tggggccggc acgcaggtac acgccgttga
     6001 ggtctgcggg aatgcggccc ttgagcaggg gcaggtcctt gagcacatgc tcgcctgcgg
     6061 tcggcgcgta cacgcccatc agcgtggggc cgtagtcctc gccaaagggg gccaggtcgt
     6121 tggggttggc gcgtgaattc atgggtctcc tccgggcgtt agattttttt actttcttaa
     6181 ctggactgac ggaatgtaag ggaaacgctt gtttcaggtc aagggttgca tggtggccgc
     6241 tggccgtccg gatttcgggt cagggggttg cggcaatcgc tgcagcgcgt gcagcctgga
     6301 tcagttcacc gatctttggg cctctagccc ccgccgccat tgcggtttct gctattgcat
     6361 tggtagcaac gcccagggcg gtctgcagtg cctgtgccag gcggcggccc tgcgggtacg
     6421 gcgtgtcctg caggccaagc cgtccccggg cgtcgcactc gcaggccagc aggatgtcgg
     6481 caaagcgtgt gggtttgcgg aaggcatcgc agcgctccag caggcgcaca gtggccgtgg
     6541 cgctcaggtc ctggctacgg tggatgttgc cgtgctcgcg tgccaccacc tgggccagct
     6601 cggcgcaatc ggtgggcacg cgcaggcgct ggcacagccc cttgagcagg cgtacgctgc
     6661 gctcctcgtg gccgatgtgg cggggcagca tgttgggcgc ggtggtgccc ttgcccagat
     6721 cgtgggtcag cgcggcaaag cgcacttcca gcggcgcgtg cagttgcgcg gccatgtcca
     6781 ccaccagcat cacatgcaca ccggtatcga cttcggggtg gtgttccacc gactgcggaa
     6841 cgccccacag ccggtccagc tcgggcagca ggtgggccag agcaccgcat tcgcgcagca
     6901 ccgcaaacat gcgcgagggt tgcgcctcca tgaggccgcg agacagctcc tgccatacgc
     6961 gttccgccac caggtggtcc acgtcgccgt ggtccaccat gtcgcgcatc agtgccatgg
     7021 tttcgggggc gaccgtgaaa tcggtaaaac gggcggcaaa gcgcgccaga cgcaggatac
     7081 gcaccgggtc ttcctggaag gcctcggtgg tgtggcgcag tacccgcgcg gcaatgtcct
     7141 gctgcccgcc ataggggtcg cacaggtttt ttaagtcaaa tgtgccatca gcccgcatgt
     7201 cctctgcgtg aatagctatg gaattgatag taaggtcgcg ccggcccagg tcttcgtcca
     7261 gcgtcacttc gggcgcatag tgcactgcaa agcccttgta accgggtgcc gtcttgcgct
     7321 cggtgcgggc cagggcgtat tcttccttgg actgtgggtg caggaacacc gggaagtctt
     7381 gccccaccgc cacatagccc tggtccagca gg
//
LOCUS       DAOSJM010000072         7399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9188_length_7399_cov_4.518382, whole genome shotgun sequence.
ACCESSION   DAOSJM010000072 DAOSJM010000000
VERSION     DAOSJM010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7399
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9188_length_7399_cov_4.518382"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /gene="mnmA"
                     /locus_tag="PKX82_03875"
     CDS             <1..652
                     /gene="mnmA"
                     /locus_tag="PKX82_03875"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426643.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOF51418.1"
                     /translation="LNQAQLSKTLFPVGELHKTEVRRIAAEIGLPNAKKKDSTGICFI
                     GERPFREFLNRYISKEPGPIKDPKGRTIGQHVGLSFYTLGQRQGLGIGGLKAKGAQKG
                     GGEHTPWFVARKDMASNTLWVVQGHDHPWLQSMSLEAQDASWVAGAPPSDAGLAAKTR
                     YRQADATCRITGATTDGFNLHFSEPQWAVTPGQSAVVYAGEVCLGGGVIASAHPGE"
     gene            complement(632..916)
                     /locus_tag="PKX82_03880"
     CDS             complement(632..916)
                     /locus_tag="PKX82_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOF51419.1"
                     /translation="MLSDSQARTDVLNRLRRAEGQIRGIQRMVEEGEDCLKIGQQFSA
                     VRKALDSTYLRMTVCFMEQELSARMEPDEAQKADLGVMLKDMEALLARMG"
     gene            complement(976..1200)
                     /locus_tag="PKX82_03885"
     CDS             complement(976..1200)
                     /locus_tag="PKX82_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOF51420.1"
                     /translation="MTIERYIRVLAGFFVMLSLALGIEGSPIFVSKWALAFTAFVGLN
                     LFQSGFTNFCAPGILLRKLGVPDNGASCGR"
     gene            complement(1231..2529)
                     /locus_tag="PKX82_03890"
     CDS             complement(1231..2529)
                     /locus_tag="PKX82_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51421.1"
                     /translation="MAHIVIVGAGIGGMPAAYEIRELLGKEHRVTVVNATDYFQFVPS
                     NPWIAVGWREREQITLPIAPYLQRKGIDFIAKPVTAIDAAANRLTLGGDQALDYDYLV
                     ITTGPKLSFSEVPGAGPASAGGGGFTHSICSVDHAQEFFADYQKFLENPGPVVIGAMP
                     GASCFGPAYEFAFILDTDLRKRKLRNKVPITYVTSEPYIGHLGLGGVGDSKSMLESEM
                     RNRDMKWITNAKTTKVEDGKLFVTQLDDLGNVYKEHEVGFKLSMMLPAFKGVDAVAAV
                     PNLCNPRGFVLIDEFQRSKAYQNIFSAGVCVAIPPVEVTPVATGTPKTGYMIESMVGA
                     IAHNIAADLAGKPATSKGTWNAICLADMGDTGAAFVALPQIPPRNVNWFKKGKWVHLA
                     KIGFEKYFMRKIRNGNSEPVYEKYVLKALGIVRLTEKSKP"
     gene            complement(2558..5797)
                     /locus_tag="PKX82_03895"
     CDS             complement(2558..5797)
                     /locus_tag="PKX82_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOF51422.1"
                     /translation="MGISGRIAAAFQSNALTPLLALVALLLGLFAVLVTPREEEPQIN
                     VTMANVLIPFPGASSVDVQNMVARPAEQVLGQIAGIEHTYSVARPGMAVLTIQFKVGV
                     PRTEALVRLYDVLNANQDWVPRELGTLTPIVKPKGIDDVPVIAVTLWSKDGLPAVELE
                     TVAHSVEAELKAAPGVREVKTIGGPGRAVQVFLDPARLRARNVDVIALRKMLGAANFG
                     MPAGAVLDNSRSQTGVQMLTVDAGEFLRSAEDVGDMVVGVSGGKPVFLRDVARVEEGA
                     QQAQRYVSFTNAGGQANPAVTISVTKKPGENAVDVSRAVRARLDALRNTVIPDNLEVT
                     VTRDYGETAAEKANKLIQKLAFATGSVILLVGFALGRREALIVGAAVILTLMATLFAS
                     WAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRHQQLTPGAALKDIIPRAVDEVGG
                     PTILATLTVIAALLPMAFVSGLMGPYMSPIPINSSLGMALSLAIAFTVTPWLALQFMK
                     EHDSHAADAGHAKPAASATKVQRLFQAVLTPLLNSARKRWLLLGGIMAALLLSVGLTL
                     VQWVVLKMLPFDNKSEFQVVVDMPAGTALEETAATLQDLGVFLAQQPEVLNLQTYAGT
                     ASPITFNGLVRQYYLRADAEQGDVQVNLVDKQHRSEKSHVIAQRLRPGLEEIGRKHNA
                     RVKVVEVPPGPPVMSPLVAEVYGPDEAGRQKLAIEVARSFGATADIVGVDTSLREDAP
                     RAFLKVRRQRAESLGIPVAAIAQTVSDALSGADAAYLHDQQSKYPVPVRMQLPPESQV
                     GLDAILALPMRAANGQLIPLSELVQVERGVIDKPLFTKNLMSVTYVMGDMAGKLDSPL
                     YGLFAIRDHLAESYKSRPDPTGEYWINQPTDPYRQYAIKWDGEWQITYETFRDMGAAY
                     AVGLVLIYLLVVAQFRSYLTPLVIMAPIPLTIIGVMPGHALLGAQFTATSMIGMIALA
                     GIIVRNSILLVDFIELQVSQGMAFKEAVLQSATVRAQPIALTGLAAMIGAFFILDDPI
                     FNGLAVSLIFGILVSTLLTLVVIPVLYYALYRRRMESRTDATGLEVNPG"
     gene            complement(5823..6932)
                     /locus_tag="PKX82_03900"
     CDS             complement(5823..6932)
                     /locus_tag="PKX82_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOF51423.1"
                     /translation="MNISSLPTAQPARKLAILGAAWVMVGAAVLGSMAHAESVPVPTV
                     AVQSQSSGAMVVLDGVIEAVKQSTVSAQASGRIATFVVKAGDKVRAGQTLATVDDREA
                     ATGVQRSQAQVNQAEAELRNAKANMERTKDLQSKGFVSKAALDSAEAQYQSAQAMRDQ
                     ATAGARQSGLAQGFTRVTAPFDGWVLQTHSQVGDLAVPGKPLMTVYAPQPLRAVVQVP
                     ASRAQSVMKATQTTIKVDNGADKPMWITPASRSAIPSADPVSQTTEWRFEIPAKDAVS
                     LVPGQQVNVQFLQAQADPVARLVVPLASVVRRGELTAVYVSTGQGFSLRAVRLGAQQS
                     GDRVEVLAGLSASDVVAMDPVRAGSTGAQPAKSSK"
     gene            7077..>7399
                     /locus_tag="PKX82_03905"
     CDS             7077..>7399
                     /locus_tag="PKX82_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51424.1"
                     /translation="MFHIIRNAEGAIHAVAKQALPGSEPMDESDPELAQFFNGGVSNP
                     GFDAADAEFVRVLEDLIDTLIMKNVIRHTDLPAAAQQKMVKRKGLRNRMQGALNLLGG
                     DGQVI"
BASE COUNT         1679 a         2234 c         2003 g         1483 t
ORIGIN      
        1 gctgaaccag gcccagctgt ccaaaacgct gttcccggtc ggcgaactgc acaaaaccga
       61 ggtccggcgc attgccgcag agatcgggct gcccaacgcc aagaagaaag actcgaccgg
      121 gatctgcttt attggtgagc gaccgtttcg cgagttcctg aaccgctata tctccaagga
      181 gcctggcccc atcaaggacc ccaaaggccg cacgattggc cagcacgtgg ggctgtcctt
      241 ctatacgctc ggacagcgcc agggcttggg cattggcggc ctcaaggcca aaggcgcaca
      301 gaagggtggc ggagaacaca ccccgtggtt tgtggcacgc aaagacatgg catccaatac
      361 cttgtgggtg gtgcaaggcc atgaccatcc gtggctgcag tccatgtcac tggaagcgca
      421 ggatgccagc tgggtcgctg gagccccccc ttcagacgcc gggttggctg caaagacgcg
      481 ataccggcag gcggacgcca cgtgccgtat taccggcgcg accacggacg gattcaacct
      541 gcatttcagc gaaccacagt gggcggttac gccaggccaa tcggccgtgg tgtatgccgg
      601 cgaggtgtgc ctgggtggag gggtgattgc ctcagcccat ccgggcgagt aaggcttcca
      661 tatctttgag catgacccca aggtcggcct tctgggcctc atcgggttcc atgcgcgcag
      721 acagttcctg ctccataaag cagactgtca tgcgcagata ggtactatcc agcgccttgc
      781 gcacagcaga aaactgctgc cctatcttca ggcagtcctc cccctcctcc accatacgct
      841 ggataccgcg tatctggccc tccgcgcggc gcagccggtt aagtacatcc gtgcgagcct
      901 gggaatcact gagcactgtc acagtacaac tttcgtcaat gggtaagacc tatgccgcat
      961 ttgcgtccga ccgacttagc gcccgcaaga ggccccgttg tcgggaacac ccagcttacg
     1021 caacagaatg ccaggagcac agaaattggt gaagcctgac tgaaacaagt tgagccccac
     1081 aaatgcagta aaagccagtg cccacttgct gacaaagatg ggactacctt cgatacccaa
     1141 tgcaagtgac agcatcacaa aaaaaccagc cagtactcgg atgtaacgtt caatggtcat
     1201 aaaaatactc ctataaaaaa atgaaacgaa tcagggtttg cttttctcgg tcaggcggac
     1261 tatgcccagt gccttgagta catacttctc atacacaggc tcggaatttc cgttgcgaat
     1321 cttgcgcatg aagtatttct caaaaccaat ttttgccagg tgaacccatt tgcccttttt
     1381 gaaccagttc acattgcggg gcgggatttg gggcagtgcg acaaacgcgg caccggtgtc
     1441 acccatgtca gccaggcaaa tggcattcca ggtgcctttg gacgtagcgg gcttacccgc
     1501 aaggtcagcg gcaatgttat gcgcaatagc accgaccatg ctctcgatca tgtacccggt
     1561 tttgggcgtc ccggtcgcca ccggcgtgac ctccacggga ggaattgcaa cgcacacacc
     1621 ggctgagaaa atattctgat aggccttgct tcgctggaat tcgtcgatca gtacaaaccc
     1681 acgcggattg cacaggttcg gcacggcagc tactgcatcc acgcccttga aggcaggcaa
     1741 catcatcgac agtttgaagc cgacttcgtg ttctttgtac acgttgccca gatcatccaa
     1801 ctgtgtgacg aatagctttc cgtcctcgac cttggtggtt ttggcgttcg taatccactt
     1861 catgtcgcgg ttgcgcattt cagactcgag catcgatttg ctgtctccca ctccccccaa
     1921 acccaaatgt ccaatgtagg gctcactggt gacataggta atgggcactt tgttgcgcag
     1981 cttgcgttta cgcaggtcgg tatcgagaat gaaggcgaat tcgtaggcgg gtccaaagca
     2041 actcgcaccc ggcatggcac caatcacgac cggcccggga ttctccagaa acttctggta
     2101 gtccgcaaag aattcttgtg catggtccac actgcagatg gaatgcgtga aaccaccacc
     2161 acctgcggag gcgggccccg ctcccggcac ctcgctgaag gagagcttgg gaccggtcgt
     2221 gatcaccagg tagtcatagt caagcgcttg gtcgccgcca agcgtcaagc ggttggcggc
     2281 tgcatcaatg gcagtgacgg gcttggcgat gaagtcaatg cccttgcgct gcaggtaggg
     2341 ggcgataggc aaagtgatct gctcgcgctc gcgccagccc accgcaatcc agggattgct
     2401 cggtacaaac tggaagtagt ccgttgcatt gacaacggtg acacggtgct cttttccaag
     2461 cagttcgcgg atttcgtagg cggcaggcat gccgccgata ccagctccga cgataacaat
     2521 atgggccatg gatttgtctc ctgagtagtt gggtgcttta gccgggattg acttcaagac
     2581 cagtcgcgtc tgtgcgcgat tccatacgac gccggtacag cgcgtaataa aggaccggga
     2641 tcaccaccag cgtgagcaag gtggatacca gaatgccaaa aatgagtgag accgccaatc
     2701 cattgaagat gggatcgtca agaatgaaga acgcaccgat catggcagcc aaaccggtca
     2761 gggcaatggg ttgcgcacgt actgtcgcgg actgcagcac agcctccttg aacgccatgc
     2821 cctgactgac ctgcagctca atgaaatcga ccagcaggat ggagttgcgc acaataattc
     2881 cggccagtgc aatcatgccg atcatcgacg tagcagtgaa ctgcgctccc aaaagagcgt
     2941 gccccggcat cacaccgatg atggtcaggg gaatcggcgc catgatcacc agaggcgtca
     3001 ggtaactcct gaactgggca accaccagca gatagatcaa caccagaccc actgcatagg
     3061 cggcgcccat gtctctgaag gtctcatagg tgatttgcca ctcaccgtcc catttgatgg
     3121 cgtattgccg ataggggtct gtcggctgat tgatccagta ttcgccagtc ggatccggac
     3181 gcgacttata gctctcggcg agatggtcgc gaatggcaaa caagccatac aacggcgagt
     3241 ccagcttgcc tgccatatca cccatgacat acgtcacgct catcaaattt ttggtgaaca
     3301 gcggcttgtc aatgactccg cgttcgacct gcaccagttc cgacagcgga atcaactgtc
     3361 catttgcagc acgcatgggc agagccagaa tggcgtcaag acccacctgg ctttccggcg
     3421 gcagttgcat ccgcaccgga accgggtatt tggactgctg gtcatgcaag taggccgcgt
     3481 cagcacccga cagagcatcc gataccgttt gggcgatggc agccaccggg atacccagag
     3541 actccgccct ctgccggcgc accttcagga atgcgcgggg agcatcttcc cgcaaactgg
     3601 tatcgacacc cacgatatct gccgtcgcac caaaggaacg cgctacttcg attgctagct
     3661 tctgccgacc agcctcatcc ggtccataga cttccgcaac cagcggtgac atgacaggag
     3721 gaccaggtgg aacctccacg actttgacgc gggcattgtg cttgcgtccg atttcttcca
     3781 gcccgggccg cagacgctgg gcgatgacat ggcttttttc actgcgatgc tgcttgtcga
     3841 ccagattgac ctgcacatcg ccttgttcag catcggcgcg caaatagtat tggcgcacca
     3901 agccattgaa cgtaatgggc gaggctgttc ctgcgtaggt ctgcaagttc agcacctcag
     3961 gctgttgggc gagaaacaca cccagatcct gcaacgtcgc tgcagtttct tccagggcgg
     4021 taccagcagg catatccacc accacctgga actcactctt gttatcaaat ggcagcattt
     4081 tcaggacgac ccattgcacc agcgtcaacc ccacagacag caacagagcc gccatgatcc
     4141 cacccagaag caaccaccgt ttgcgcgcac tgttgagcaa aggcgtcaga acggcctgga
     4201 acaatcgttg cactttggtg gccgatgccg ctggttttgc gtgcccggca tccgccgcat
     4261 gagaatcgtg ctctttcatg aactgcagag ccaaccaggg cgtcacggtg aaggcaatcg
     4321 ccagtgataa cgccataccc agactggagt tgatcggaat cgggctcatg tagggcccca
     4381 tcaagcccga cacgaaggcc atgggcagta atgccgcaat gacggtcagc gtggccagaa
     4441 tggtggggcc tccgacttca tcgacggccc ggggaatgat gtccttcagt gctgctccgg
     4501 gtgtgagctg ctggtgtcga tggatgttct ccaccaccac gatggcatcg tccaccagaa
     4561 tcccgatgga aaaaatcaat gcaaacaaag acacgcgatt gagcgtgaaa ccccaagccc
     4621 aggaggcaaa caaagttgcc atcagcgtca atatcactgc agcacccaca atcaaggctt
     4681 cccgacggcc cagggcgaaa cccaccagca aaatcaccga gcccgttgca aatgcgagct
     4741 tctgaatcag cttgttggct ttctctgctg cagtttctcc gtagtctcgc gtaacagtga
     4801 cctcaaggtt gtcggggatg acggtgtttc gcagcgcgtc caggcgtgcg cgaactgcgc
     4861 gcgatacatc cacggcgttc tcacctggct ttttggtcac actgatcgtg actgccgggt
     4921 tggcctgacc gcccgcattc gtaaaagaca cgtaacgctg ggcctgctgc gcgccttctt
     4981 ccacacgggc gacatcgcgc agaaagaccg gcttgcctcc cgagacgccc accaccatgt
     5041 ctcccacatc ttccgctgac cgcagaaatt cgccagcatc caccgtcagc atttgcacac
     5101 ctgtctgcga cctggagtta tccagcacag cacccgctgg catcccgaag ttggcagcgc
     5161 ccagcatctt gcgcagtgca atcacatcca cgttgcgggc gcgcagacgc gcaggatcca
     5221 ggaatacctg tactgcgcgc cctggaccgc cgatggtttt gacctcgcgc acgccaggtg
     5281 cggccttgag ctccgcctcg accgaatggg cgacggtttc aagttctacc gcaggaaggc
     5341 cgtctttgct ccagagcgtt accgcaatca ccggaacatc gtcaatccct ttcggcttga
     5401 cgatgggcgt cagggttccc aactcgcgcg gcacccaatc ctgattggca ttgagcacgt
     5461 catacagccg caccaatgcc tcagtgcgcg gtaccccaac cttgaattgg atggtcagca
     5521 cagccatgcc tggccgcgct acggaatatg tgtgctcgat cccagctatc tgccctagca
     5581 cctgttctgc cgggcgcgcc accatattct gaacgtccac actactggct cccgggaagg
     5641 ggatcagtac atttgccatg gtgacgttga tctgtggctc ttcttcacgt ggcgtgacca
     5701 gtacggcaaa caagcccagt agcagcgcga ccagggcaag cagcggcgtc agcgcgttgg
     5761 actgaaaggc tgcagcgatg cgacctgata tgcccatttt gttttgctca gtcatggctg
     5821 actcacttgg aagacttggc tggctgtgca ccagtcgaac ccgcgcgcac aggatccatt
     5881 gcaaccacat cgctggcaga caggcctgcc agcacctcta cgcgatcgcc actctgttgc
     5941 gcgccaaggc ggactgcgcg caatgaaaaa ccctgtccgg ttgacacata gacagctgtc
     6001 aactcaccac gccgcacaac agaagccagt ggaaccacaa gcctggcaac cgggtccgcc
     6061 tgcgcttgca gaaactggac attgacctgc tggccgggaa caaggctgac ggcgtccttc
     6121 gccgggatct caaaacgcca ctccgttgtt tgcgaaaccg ggtctgcaga cggaatagcc
     6181 gaccggctgg ccggcgtaat ccacatcggc ttgtccgctc cattgtcgac cttgatcgtg
     6241 gtctgcgtcg ccttcatcac actttgcgcg cgggatgctg gcacctgaac cacagcacgc
     6301 aggggttgtg gcgcatagac cgtcatcaac ggtttgccgg ggaccgccag atcgcctacc
     6361 tgcgaatggg tttgcagaac ccatccgtca aaaggcgcag tgacccgggt aaatccctgt
     6421 gcgagtccag attgacgcgc tccggcggta gcctggtcac gcattgcctg ggcactttgg
     6481 tactgcgctt cggcgctatc cagcgcagcc ttgctgacaa agcccttcga ttgaaggtct
     6541 ttggtgcgct ccatattggc tttagcattg cgcaactcgg cctccgcctg gttgacttgc
     6601 gcctgactcc gctgaacgcc cgtagcagcc tcccggtcat caaccgttgc caacgtttga
     6661 ccagcacgta ccttgtcacc ggccttgacc acaaacgtgg caatgcgccc tgacgcctga
     6721 gccgaaacgg tgctttgctt gaccgcttca atcactccat ccaataccac catcgcgccc
     6781 gacgattggg actgaacagc gaccgtagga accggcacag attcagcatg agccatggag
     6841 ccaagcacag ccgcaccaac catcacccac gccgcgccca agatagcgag cttgcgagcc
     6901 ggttgcgcgg tgggcaatga agatatgttc atgtttattt ccaaatacta ggtacagtat
     6961 tttcatatac tacctggggt atacataaat gtcaaggacc atccaatcga cgccgtcgga
     7021 ctgataacat cagacaaaca ctccacgcca acctccaaag aaactgccaa gcaaacatgt
     7081 ttcacatcat ccgcaacgcg gaaggagcca tccatgccgt cgccaaacaa gctctgccag
     7141 gcagcgaacc tatggatgaa agtgatcctg aactggcgca gtttttcaat ggcggggtat
     7201 caaacccggg attcgacgca gccgatgctg aatttgtccg tgtactggaa gacttgattg
     7261 acacgctgat catgaaaaac gtgattcgcc ataccgacct gcctgcagcc gcgcaacaaa
     7321 aaatggtgaa gcggaaagga ttgcgcaatc gaatgcaagg tgcgttgaac cttctcggtg
     7381 gcgatggtca agtcatttg
//
LOCUS       DAOSJM010000073         7397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9192_length_7397_cov_3.535685, whole genome shotgun sequence.
ACCESSION   DAOSJM010000073 DAOSJM010000000
VERSION     DAOSJM010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9192_length_7397_cov_3.535685"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PKX82_03910"
     CDS             <1..146
                     /locus_tag="PKX82_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOF51425.1"
                     /translation="ARKDSKYVSPGDQVRVKARDRMAVVKMDATREAPAPTQAATQAE
                     TKA"
     gene            143..451
                     /gene="nuoK"
                     /locus_tag="PKX82_03915"
     CDS             143..451
                     /gene="nuoK"
                     /locus_tag="PKX82_03915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOF51426.1"
                     /translation="MTLTLGHFLSLGGMLFALSVVGIFLNRKNLIVLLMAIELMLLAV
                     NTNFVAFSHYLHDLHGQIFVFFILTVAAAESAIGLAILVLLFRNKSSINVDELNTLKG
                     "
     gene            456..2486
                     /gene="nuoL"
                     /locus_tag="PKX82_03920"
     CDS             456..2486
                     /gene="nuoL"
                     /locus_tag="PKX82_03920"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOF51427.1"
                     /translation="MSQTLSASTLLAVPLAPLVGSALAGIFGTKFGGNWIGRRLSHTL
                     TILGVLVAFILSALTLKSVAMEGARFNETLYTWMVVGGLKMEIGFLVDGLTAMMMCVV
                     TFVSLMVHIYTIGYMDEDEGYNRFFAYISLFTFSMLMLVMSNNLLQLFFGWEAVGLVS
                     YLLIGFWFNKPTAIFANMKAFLVNRVGDFGFILGIGLIVAFGGTLNYTEVFAKAGELS
                     AMAFPGTDWMLVTVICICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTA
                     GIFMVARMSPLFELSDTALNFILVIGAITALFMGFLGIIQNDIKRVVAYSTLSQLGYM
                     TVALGASAYSVAVFHLMTHAFFKALLFLGAGSVIMGVHHNQDIRWMGGLRKYMPITWI
                     TSLLGSLALIGTPLFAGFYSKDSIIEAVLESHLPAAGFASFAVLAGVFVTAFYSFRMY
                     FLVFHGKERFDQNPDAHHDDHHDAHGHDDHHGHHKPHESPWVVTVPLVLLAIPSVVIG
                     YLAIGPMLYGEFFKDAIFVNASKHPVMEELAHAFHGPLAMAAHAFSTLPFWLALAGVA
                     VAYYMYLVNPAVPAAIKRNVMPVFKLLENKYYLDWINENILARGARALGTGLWKGGDQ
                     TLIDGLVVNGSWKVVGAIAGVARKLQTGYLYHYALVMILGIFVLMTYFVWLK"
     gene            2499..3974
                     /locus_tag="PKX82_03925"
     CDS             2499..3974
                     /locus_tag="PKX82_03925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOF51428.1"
                     /translation="MGLLSLAIWTPIAFGVILLALGRDDQPGMVRWVALIGSIISLLV
                     TLPLYGQFNTASAAMQFVEKASWIERFNVNYHLGVDGISFWFVLLTAFINVIVVIAGW
                     EVITRRVNQYMGAFLILSGLMIGVFCALDGLLFYVFFEATLIPMYLIIGIWGGPNKIY
                     AAFKFFLYTLMGSLLMLVALIYLYTASGGSFDLAVWHALPLGKTAQTFLFFAFFAAFA
                     VKVPMWPVHTWLPDVHVEAPTGGSAVLAAIMLKLGAYGFLRFSMPIAPDAAHEWAWLM
                     ITLSLVAVVYVGLVAMVQQDMKKLVAYSSVAHMGFVTLGFFIFNDLGISGGLVQMIAH
                     GFVSGAMFLSIGVLYDRVHSREIASYGGVVNTMPKFAAFGLLFAMANCGLPGTAGFVG
                     EWMVILGAVKFNFWIGFAAASALIFGAAYTLWMFKRVYLGPVANDDVKALVDINAREF
                     TMLALLAIAVLYMGIYPKPFTDVMDASVAQLLKHVAISKLN"
     gene            3999..5486
                     /gene="nuoN"
                     /locus_tag="PKX82_03930"
     CDS             3999..5486
                     /gene="nuoN"
                     /locus_tag="PKX82_03930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HOF51429.1"
                     /translation="MIDKISWIAVYPEIILFAMACAIALLDLGVKSPLRGRTYALTLL
                     TLAVVAILQAAYATGGATMYAFGNMVVSDSMGNWLKCFATVAVMVTLVYGRPYAGQRD
                     MLRGGEFFTLSMFALLGMFVMISGNNLLVIYMGLELLTLSSYALVALRRDNATATEAA
                     MKYFVLGALASGFLLYGMSMMYGATGSLDINMIFKAIVSGQVRHQVLVFGLVFIVAGL
                     AFKLGVVPFHMWIPDVYQGAPTAITLMIGGAPKLAAFAIVIRLLVEGMLPLAFDWQQM
                     LMVLAVGSLLIGNLAAIAQTNVKRMLAFSTISQMGFVLLGLMSGVVNGNTLSAANAYS
                     SAMFYVVSYVLTTLASFGVLLLLSREGFESEEIADFAGLNQRSPLYAGVMAVCLFSLA
                     GIPPMVGFYAKLSVLQALVASGQALYLGLAIFAVLMSLIGAFYYLRLIKVMYFDEPIT
                     ATHVVAPMDVRAVLSINGALVLILGLLPSGLMDICARSVLQMLGT"
     gene            5520..5822
                     /locus_tag="PKX82_03935"
     CDS             5520..5822
                     /locus_tag="PKX82_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HOF51430.1"
                     /translation="MTTSVSVWLVLVLALVAANLPFFSQKLFGVIARPSPKSLGVRLL
                     ELLALYLLSGLVGLFLENRAGQIVPQTWEFYAITVTLFLTFAFPGFVYRYLLRHRD"
     gene            5836..6438
                     /locus_tag="PKX82_03940"
     CDS             5836..6438
                     /locus_tag="PKX82_03940"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOF51431.1"
                     /translation="MGDSHLIETRTSSQEVLKGVFLHAFRDTVRLPDGQSSVREYVVH
                     PGAVMVIPLLTDAQGQVRVVLERQFRYPVGQVMIEFPAGKLDADESVLACAQRELREE
                     TGYTATEWARAGVMHPVIAYSTEFIDIWFARGLTLGERKLDQGEFLDVITATPQELLQ
                     WCFEGKVTDAKTLTGALWLQNVLAGAWALPWQNSGEALAG"
     gene            6449..6898
                     /locus_tag="PKX82_03945"
     CDS             6449..6898
                     /locus_tag="PKX82_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOF51432.1"
                     /translation="MKVLDLQCSNGHTFEGWFASEDDFQTQLAQSLVQCPLCGHPDVV
                     KKLSAPRLSLSGAQHAPAKESTEAVPVASQPALAAAWMAAARHVMANTTDVGNRFAEE
                     ARKMHYGEAEERAIRGKTTPDEAQALVEEGIDVVPLLIPDALKEPLQ"
     gene            complement(6922..>7397)
                     /locus_tag="PKX82_03950"
     CDS             complement(6922..>7397)
                     /locus_tag="PKX82_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HOF51433.1"
                     /translation="AHVALTGSVAGFAHAEVISQDAFWDVDCDILIPAALEQQITAAN
                     AGRIQARLVIEGANGPTTPQADDILQERGILVLPDVIANAGGVTVSYFEWVQDFSSFF
                     WSEDEINARLVRIMKEAFAGVWQVAQDHKVSLRTATFIVACKRILHARELRGLYP"
BASE COUNT         1290 a         2022 c         2205 g         1880 t
ORIGIN      
        1 gtgcccgcaa ggacagcaaa tatgtcagcc caggcgatca ggtgcgcgtc aaggcgcgcg
       61 accgaatggc cgtggtcaag atggatgcca cacgtgaggc tcccgcgccc acacaggctg
      121 ctacgcaagc ggagaccaaa gcatgacttt gacacttgga catttcctct cactgggtgg
      181 catgctgttt gccctgtcgg tggtgggcat cttcctgaac cggaagaacc tgatcgtgtt
      241 gttgatggct attgaactga tgttgctggc tgtcaatacc aatttcgtcg ccttttcgca
      301 ttacctgcat gatctgcacg gtcagatatt cgtgttcttc attctgacag tggctgccgc
      361 ggagtccgcg atcggattgg ccattctggt gctgctgttc cgtaacaagt ccagcatcaa
      421 tgtggacgaa ctcaatacgc tcaagggtta ataagatgag tcaaaccctg tctgcttcta
      481 cactgcttgc ggtcccgctg gcacccttgg tgggatccgc tctggcgggt atttttggca
      541 ccaaatttgg tggcaactgg atcggtcgcc gtctgagtca tacgctgacc attcttggcg
      601 tactggtcgc ttttatcctg tccgcgctga cactcaagtc ggttgccatg gaaggggccc
      661 gttttaacga gaccctttac acctggatgg tcgtcggcgg actgaagatg gagatcgggt
      721 tcctggtcga cggcctgacc gccatgatga tgtgcgtggt cactttcgtc tcgctgatgg
      781 tgcacatcta caccattggc tatatggacg aagacgaggg ctacaaccgt ttcttcgcct
      841 acatctcgtt gttcaccttc tccatgttga tgctcgtcat gagcaacaac ctgctgcaac
      901 tgttcttcgg ttgggaagca gtgggtttgg tgtcctattt gctgattgga ttctggttca
      961 acaagcccac agccattttt gccaatatga aggcgttctt ggttaaccgt gtgggtgact
     1021 tcgggttcat tcttggcatc ggtttgattg ttgcctttgg tggcaccctc aactacacag
     1081 aagtattcgc gaaggcgggt gaactgtctg caatggcatt tcctggcacg gactggatgc
     1141 tggtgaccgt catctgcatt tgcctgttta ttggtgccat gggcaaatcg gcgcagttcc
     1201 cgctgcatgt gtggctgccg gactccatgg aaggccccac tccgatttcc gcgctgattc
     1261 acgccgcgac gatggtgacc gctggtattt tcatggttgc ccgcatgtcg ccgctgttcg
     1321 aacttagcga cacagcactc aacttcattc tggtgatcgg cgccatcacg gccctgttca
     1381 tgggcttcct gggcatcatt cagaatgaca tcaagcgtgt ggtggcctat tccacgctgt
     1441 cgcagctcgg gtacatgaca gtggcgctgg gtgcctcagc ctactcggtc gcagtattcc
     1501 acctgatgac acacgctttc ttcaaggcgt tgctgttcct cggcgcgggc tcggtcatca
     1561 tgggtgtgca ccacaaccag gacatccgct ggatgggtgg tctgcgtaag tacatgccca
     1621 ttacctggat cacgtcgctg ctcggttcgc tggcactgat tggcacgccg ctgtttgccg
     1681 gtttctattc caaggacagc attattgagg cggttctcga aagccacctg ccagctgccg
     1741 gatttgcatc tttcgccgtt ctggctggcg tgtttgtcac agctttttat tcgttccgca
     1801 tgtatttcct ggtgttccac ggaaaagagc gcttcgacca aaatccggat gctcaccacg
     1861 acgatcacca tgatgcgcac gggcatgatg accatcacgg acaccacaag ccgcacgagt
     1921 cgccctgggt cgtgaccgta ccgctggtac tgctggccat tccctccgtg gtgattggtt
     1981 acctggcgat cggtcccatg ttgtatggag agtttttcaa agacgccatc ttcgtgaacg
     2041 cttcgaaaca ccctgtgatg gaggagttgg cccacgcatt ccacggtccg ctggccatgg
     2101 cggcccacgc cttcagcacc ttgccgttct ggcttgcgct ggcgggtgtt gccgttgcct
     2161 actacatgta tctggtgaac ccggctgtgc ccgcagcgat caagcgcaat gtcatgccgg
     2221 tcttcaaact gctggagaac aagtactacc tcgattggat caacgagaac attctggccc
     2281 gtggtgcccg agctctggga accggtctgt ggaagggtgg ggaccagacg ctgattgacg
     2341 gtctggtggt caatggttca tggaaagtgg tgggcgccat tgccggcgtt gcgcgcaagt
     2401 tgcagacggg ttacctgtac cactacgccc tggtcatgat tttgggcatt tttgtgctca
     2461 tgacgtattt cgtctggctc aagtaggaga aaaagaacat gggattgttg agccttgcga
     2521 tctggacgcc cattgcgttc ggagtgattt tgctggcgtt gggacgcgac gaccaacccg
     2581 gtatggtgcg ttgggtggcc ttgattggct cgatcatcag cctgctggtg acgctgccgc
     2641 tgtacggaca gttcaatacc gcgtctgcag ccatgcagtt cgttgagaaa gcctcctgga
     2701 ttgagcgctt caatgtcaat taccacctcg gtgtggatgg catttctttc tggtttgtgt
     2761 tgctgacggc atttatcaac gtgatcgttg tcatcgccgg ttgggaggtc atcacccgcc
     2821 gcgtcaacca gtacatgggc gccttcctga ttctgagcgg tttgatgatc ggcgtgttct
     2881 gtgcgctcga tggtctgttg ttctacgtct ttttcgaagc cacgctgatc ccgatgtacc
     2941 tgattattgg tatctggggc ggtccgaaca agatctatgc cgcgttcaag ttcttcctgt
     3001 atacgctgat gggttcgctg ctcatgctgg tggcactgat ttacctgtac accgcgtcgg
     3061 gcggcagctt tgatctggcg gtgtggcacg cactgccgct tggcaagact gctcagacgt
     3121 tcctgttctt tgccttcttc gcagcattcg ccgtcaaggt gcctatgtgg ccggtgcaca
     3181 cctggttgcc cgatgtccac gtggaagcgc ctacgggtgg ttccgcggtt ctggccgcca
     3241 tcatgctgaa gctcggagcc tatggtttcc tgcgcttctc gatgccgatt gcgcccgacg
     3301 cagcacatga atgggcctgg ctgatgatca cactgtcact ggtggcggtc gtctacgtcg
     3361 gtctggtggc catggtgcaa caggacatga agaagctggt ggcttattcc tctgtggccc
     3421 atatgggttt tgtgacgctg ggttttttca tcttcaatga tctgggtatt tccgggggac
     3481 tggttcagat gattgcgcac ggttttgtgt cgggcgccat gttcctgtcc atcggtgtac
     3541 tgtatgaccg ggtgcactcg cgcgaaatcg cgagctatgg cggcgtggtc aacaccatgc
     3601 ccaaattcgc ggcatttggc ctgctgtttg ccatggccaa ctgcggtttg ccgggtaccg
     3661 ctggattcgt aggtgagtgg atggtgattt tgggtgcagt caagttcaac ttctggatcg
     3721 gttttgcggc cgccagtgcg ctgatttttg gtgcggccta tacgctatgg atgttcaagc
     3781 gcgtgtacct cggcccggtc gccaatgacg atgtgaaggc cttggtcgac atcaacgcgc
     3841 gtgagttcac catgctggcg ctgctggcca ttgcggtgct ctacatgggc atttatccca
     3901 agccgtttac tgacgtcatg gatgcttcgg ttgcgcagct gctcaagcat gttgcgattt
     3961 ccaagctgaa ctgatcaaac tgaattgaga gacctaagat gattgacaaa atcagttgga
     4021 tcgccgttta ccccgagatc attctgtttg ccatggcgtg cgccatcgcg ctgctggatc
     4081 tgggcgtcaa gtcgcccctg cgtgggcgga cctatgcact aaccctgctg accctggccg
     4141 tggtggccat ccttcaggcg gcctacgcta cgggcggggc caccatgtac gcgttcggca
     4201 acatggtggt cagtgactcc atgggcaatt ggctcaagtg ttttgccact gtggcagtca
     4261 tggtgacact ggtttatggt cgcccttatg cgggacagcg ggatatgctg cgcggcggtg
     4321 agttcttcac actgagcatg tttgcccttc tcggtatgtt cgtgatgatt tccgggaaca
     4381 acctcctggt gatctacatg ggactggagt tgctgaccct gtccagttac gccctcgtgg
     4441 ctttgcgccg cgacaacgcg acggcgaccg aagctgccat gaagtacttt gtgctgggcg
     4501 ccctggcttc gggcttcctg ctgtacggta tgtcgatgat gtatggcgcc acaggttcgc
     4561 tggacatcaa tatgattttc aaggccatcg tttccggcca agtgcgccac caggtgttgg
     4621 tgtttggttt ggtgtttatc gtcgccggcc tggcgttcaa gctcggtgtg gtgcctttcc
     4681 atatgtggat ccccgacgtg taccagggcg cccccaccgc cattacgctg atgattggcg
     4741 gcgctcccaa gcttgcggca tttgccatcg tgattcggct gctggtggaa ggcatgctgc
     4801 cgctggcctt tgactggcaa caaatgctga tggtgctggc ggtaggttcg ctgttgattg
     4861 gtaacctggc ggccatcgcg cagaccaatg tcaagcgcat gctggccttc tccacgattt
     4921 cccagatggg ttttgtgctg ctgggtttga tgtctggtgt tgtcaatggc aataccctgt
     4981 ctgcagccaa tgcctacagc tcggcgatgt tctatgtggt cagctacgtt ctcacgacac
     5041 tggccagctt cggtgtgctg ctgctgttgt cccgcgaggg tttcgagagc gaagagattg
     5101 cggactttgc cggtttgaac cagcgcagtc ccctgtatgc cggtgtgatg gctgtctgcc
     5161 tgttctccct ggcaggcatc ccgcccatgg tcggtttcta cgccaagctg tcagtgctcc
     5221 aggcattggt ggcgtccggt caggcgctgt acctcggcct ggcgatcttc gctgtcctga
     5281 tgtccctcat cggtgctttc tactacctgc gactcatcaa ggtcatgtac tttgacgagc
     5341 cgatcacggc cacccatgtg gttgctccca tggatgtgcg cgcagtcttg agcatcaacg
     5401 gggctctggt gctcattctg ggcttgctgc ccagcggtct catggatatt tgcgcgcgtt
     5461 cggtgctgca aatgctggga acctgagcgg tttttggctg ctgatctgag tctgcctgcg
     5521 tgaccacttc tgtttctgtc tggctggtac tggtgctcgc attggtggca gcgaacttgc
     5581 catttttcag ccagaagctg ttcggcgtga tcgcacggcc aagccccaag tcattgggtg
     5641 tgcgtctgct ggagttgctg gcgctctatc tgctgtcggg tctggtcgga ttgtttctcg
     5701 aaaaccgtgc tggccagatt gttccgcaga cttgggagtt ctacgccatc acggtcacac
     5761 tcttcctgac ctttgcgttc cctggcttcg tctaccgcta tctgttgcgg cacagagatt
     5821 aatctggcgg gagccatggg cgatagccac ctgattgaaa cccggacgtc gagccaggaa
     5881 gttctcaagg gcgtctttct gcacgcattt cgcgataccg tgcggttgcc ggatgggcag
     5941 agttccgtgc gcgaatatgt ggtgcacccg ggggccgtca tggtcattcc tttgctcacg
     6001 gatgcacagg ggcaggttcg tgtggtgctg gagcggcaat tccgttatcc cgtcgggcag
     6061 gtgatgatcg aatttccggc cggcaagctc gacgccgatg aaagtgtgct ggcctgtgcg
     6121 caacgggagc tgcgcgaaga gaccggttac accgccaccg agtgggcccg tgcgggtgtg
     6181 atgcacccgg tcattgcgta ctccacggaa ttcatcgaca tctggtttgc ccgaggcctg
     6241 accctggggg agcgcaagct cgaccagggg gagtttctgg acgtaatcac tgcgactccg
     6301 caggaactgt tgcaatggtg ttttgaaggc aaggtcaccg atgccaagac gctgacgggg
     6361 gctttgtggt tgcagaacgt acttgcgggc gcatgggctt taccgtggca gaacagcggc
     6421 gaagcactgg cgggttgaca atacgtgcat gaaagttctg gatctgcaat gctcgaacgg
     6481 ccacaccttt gagggctggt ttgcgtctga agacgatttt cagacgcagc ttgcgcaatc
     6541 cctggtgcag tgccctctat gtgggcaccc tgacgtggtc aagaaactca gtgcaccccg
     6601 acttagcctt tccggcgcac agcatgcacc cgcaaaggaa tcaaccgaag cggtacccgt
     6661 agcgagccag cctgccctgg ctgccgcatg gatggctgca gcccgccatg tgatggccaa
     6721 caccaccgat gtaggcaacc gctttgccga ggaggcccgc aagatgcact acggcgaagc
     6781 cgaggagcga gccatccgcg gcaagaccac cccggacgag gcgcaggcgc tggtcgaaga
     6841 agggatcgac gtggttccgc tgctgatacc ggatgcgttg aaggaacccc tgcagtaaat
     6901 ccttgcagga gcacttcatg ctcaggggta cagaccccgc aactcgcgtg cgtgcagtat
     6961 tcgtttgcag gccacgatga aagtggcggt acgcaggctg accttgtggt cctgtgccac
     7021 ctgccacaca ccggcaaatg cttccttcat gatgcgtacc agacgggcat tgatttcgtc
     7081 ttcgctccag aaaaagctgg agaaatcctg cacccactcg aagtagctga ccgtcacgcc
     7141 acctgcgttg gcaatgacgt cgggtagcac cagaatgccg cgttcctgca ggatgtcatc
     7201 ggcctgtggt gtggtcggtc cattggcacc ctcaatcacc agacgggctt ggatgcgccc
     7261 tgcattggct gccgtgatct gctgttccag tgcggcagga atgaggatgt cgcagtccac
     7321 atcccagaat gcatcctggg agatcacctc cgcgtgtgcg aagcccgcca cggagccggt
     7381 cagtgcgacg tgggcca
//
LOCUS       DAOSJM010000074         7357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9256_length_7357_cov_4.684607, whole genome shotgun sequence.
ACCESSION   DAOSJM010000074 DAOSJM010000000
VERSION     DAOSJM010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9256_length_7357_cov_4.684607"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>300)
                     /locus_tag="PKX82_03955"
     CDS             complement(<1..>300)
                     /locus_tag="PKX82_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOF51434.1"
                     /translation="GAQITVPVGKATLGRIMDVLGTPIDERGPVSQEQTASIHRKAPA
                     YDELSPSQELLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMMELINNIAKAH"
     assembly_gap    301..310
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<311..526)
                     /locus_tag="PKX82_03960"
     CDS             complement(<311..526)
                     /locus_tag="PKX82_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOF51435.1"
                     /translation="MQAQGKIVQCIGAVVDVEFPRDKMPRVYDALKMEGSPLTLEVQQ
                     QLGDGIVRTIALGSSDGLRRGLVVYNTG"
     gene            complement(555..1439)
                     /gene="atpG"
                     /locus_tag="PKX82_03965"
     CDS             complement(555..1439)
                     /gene="atpG"
                     /locus_tag="PKX82_03965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOF51436.1"
                     /translation="MAAGKELRTKIKSVENTKKITKAMEMISVSKMRKAQERMRAARP
                     YSTKIRDIATNLGQANPEYVHAFMKTNDAKSVGLIVVSTDKGLCGGLNTNLLRLVTGK
                     LRETQTAGKTTHAVAIGSKGLGFLNRVGAKVVSHVTHLGDRPHLDKLIGPVKVLLDAY
                     VKGEVSEVHICYNKFVSTMKQDPVVEQLLPLSSAKMAAESKAEGGGHAWDYIYEPDAQ
                     VVIDELLVRYVEALVFQALAENMASEHAARMVAMKAATDNAGSVIGELKLIYNKTRQA
                     AITKELSEIVSGAAAISG"
     gene            complement(1467..3020)
                     /gene="atpA"
                     /locus_tag="PKX82_03970"
     CDS             complement(1467..3020)
                     /gene="atpA"
                     /locus_tag="PKX82_03970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOF51437.1"
                     /translation="MQLNPAEISELIKSRIEGLSASADIRNQGTVISVTDGIVRIHGL
                     SDVMQGEMLEFPAGSDGLPTFGLALNLERDSVGAVILGEYEHISEGDTVKCTGRILEV
                     PVGPELIGRVVNALGQPIDGKGPINAKMTDVIEKVAPGVIARKSVDQPMQTGLKSIDS
                     MVPIGRGQRELIIGDRQTGKTAVAIDAIINQKGQNMTCVYVAIGQKASSIKNVVRALE
                     QAGAMEYTIVVAASASESAAMQYVSAYSGCTMGEYFRDRGQDALIVYDDLSKQAVAYR
                     QVSLLLRRPPGREAYPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPI
                     IETQAGDVSAFVPTNVISITDGQIFLETSLFNAGIRPAINAGISVSRVGSAAQTKVVK
                     GQSGGIRNDLAQYRELAAFAQFASDLDEATRKQLDRGARVTELLKQKQYSPQSISLMS
                     ASLFAVNKGFMDDVEVKKILAFEHGLQSYLKDKHAALLAKVESTQALDKDAEAELTAA
                     VAAFKKTFA"
     gene            complement(3070..3600)
                     /locus_tag="PKX82_03975"
     CDS             complement(3070..3600)
                     /locus_tag="PKX82_03975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOF51438.1"
                     /translation="MAELATIARPYADALFKASVQDLSATAAWVDELAAVAGNAQLLQ
                     FADSPKVTAAQVFDLITGVAKAALNDHAKNFLRMVIENGRLSALPEIAAQFRALKNAQ
                     SGSSDAVVYSAFPIEGAALADVSNMLEKRFGRKLNVSVELQSELIGGIRVVVGDEVLD
                     TSVKARLEQMKVALTA"
     gene            complement(3610..4080)
                     /locus_tag="PKX82_03980"
     CDS             complement(3610..4080)
                     /locus_tag="PKX82_03980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOF51439.1"
                     /translation="MSINATLFVQAIVFAILVWFTMKFVWPPIAAALDERAKKISDGL
                     AAADKAKSELSAANKRVEDELAKSRNENAARLADAERRAQAIVEEAKAKATEEGNKII
                     AAAKVEAEQQTVKARESLREQVAALAVKGAEQILRKEVNAGVHADLLGRLKTEL"
     gene            complement(4112..4360)
                     /gene="atpE"
                     /locus_tag="PKX82_03985"
     CDS             complement(4112..4360)
                     /gene="atpE"
                     /locus_tag="PKX82_03985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HOF51440.1"
                     /translation="MEHVLGFVALAAGLIIGLGAIGACIGIGIMGSKYLEAAARQPEL
                     MNELQGKMFVLAGLIDASFLIGVGIAMMFAFANPFVLK"
     gene            complement(4408..5277)
                     /gene="atpB"
                     /locus_tag="PKX82_03990"
     CDS             complement(4408..5277)
                     /gene="atpB"
                     /locus_tag="PKX82_03990"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOF51441.1"
                     /translation="MSAEAAVAEGAAHQGPTAGEYIVHHLTHWQNQPMKGVIDFSVYN
                     YDSIFWSVLLGVVGCFFLWRAAKGAHAGVPGRFQAAVELLVEFVDEQAKGIVHNAQSR
                     KMVAPLALTVFVWIFLMNSMDFLPVDLLPKLWLMLSGGDEHHTYMRVVPTADLSVTLG
                     MSVSVLLVCIYYNIKIKGLGHWIHELFSAPFGAKLGPINFLFQMIEFVAKTVSHGMRL
                     FGNMYAGELVFMLIALMGSALAASTTGIMLPIAHIIAGSVWSIFHILIVGLQAFVFMM
                     LTLVYVGQAHDAH"
     gene            complement(5295..5762)
                     /locus_tag="PKX82_03995"
     CDS             complement(5295..5762)
                     /locus_tag="PKX82_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOF51442.1"
                     /translation="MTTMPPEADEDGEVSDFVPLTAEQAQTLREQNPSTSPWWVVLGQ
                     VLVGVLVTLAAWGISGKTSVAWSAAFGALAVVIPAALFARGVAGQFASANAGAAVMGF
                     FLWEFVKIVLTVSILFVAHRLVMNLNWPVMLVSLVVTMKVYWLALIRHPKRKH"
     gene            5902..6801
                     /locus_tag="PKX82_04000"
     CDS             5902..6801
                     /locus_tag="PKX82_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HOF51443.1"
                     /translation="MHNLPRLPCYLNGEYTVLPEAKVSVMDRGFIFGDGVYEAIPVYA
                     GQPFRFAHHMARLERSLQETRIPQPHSTAQWQEIALSLIAAYAHSTGASTQNTDQFVY
                     LQVTRGVAMRDHVMPQGIAPTVFAMTNAMKPPTAQQRADGVACVTAEDFRWEKAHIKS
                     TSLLGAVMARQTSFDAGAVETVMFRRGFLSEAAASNVWVIKHGVVMGPPKDHLVLEGI
                     RYGLIEEICQAADIPFVLRPVAKSEVLDADELLLSSATKEVLPITTLDGQPVGNGRPG
                     PIYQALYEGYQRIKAQSAASATH"
     gene            6815..7096
                     /locus_tag="PKX82_04005"
     CDS             6815..7096
                     /locus_tag="PKX82_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HOF51444.1"
                     /translation="MSLPEIPPSESLITYPSQFPIKIMGVSTEGFVHAITTIAHAFDP
                     AFDASTIELRESKGGKYLGVTITITATSREQLDELYRTLSTHPMVKVVL"
     gene            7101..>7357
                     /locus_tag="PKX82_04010"
     CDS             7101..>7357
                     /locus_tag="PKX82_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51445.1"
                     /translation="MDFKVSNLGRVDYLPTYAAMQAFTDQRDVGPASTVQDQLWICEH
                     PPVFTQGLAGKAEHIFNPANIPVVQTNRGGHAIAIQLQLSG"
BASE COUNT         1661 a         2274 c         1945 g         1467 t
ORIGIN      
        1 tgtgcgcctt ggcgatgttg ttgatcaact ccatcatgtt cacggtcttg cccacaccgg
       61 caccaccgaa cagaccgact ttaccgccct tggcgaacgg gcaaaccaag tcaatcacct
      121 tgatgccagt ttccagcagt tcctgggaag ggctcagctc gtcatacgca ggagccttgc
      181 ggtgaatgga agccgtttgc tcctggctca cggggccgcg ctcgtcgatc ggcgtaccca
      241 gcacgtccat gatgcggccc agtgtggcct tgcccacggg aaccgtgatt tgcgcgccgg
      301 nnnnnnnnnn gccggtgttg tacaccacca gaccacgacg caggccgtca gacgaaccca
      361 gcgcaatggt acgcacgatg ccatcgccca gctgctgctg cacttcgaga gtcagcggag
      421 agccttccat cttgagtgcg tcatacacgc gaggcatctt gtcacgggga aactccacgt
      481 ccaccaccgc gccaatacac tgaacaattt ttccttgggc ttgcattttt cgctccaata
      541 atatttaaat tcgcttagcc gctgatggca gctgcaccag acacgatttc agacagctct
      601 ttggtaatcg cagcctgacg ggtcttgttg tagatgagct tgagttcgcc aatgacgctg
      661 ccggcattgt cggtggcagc cttcatggcc accatccgcg cagcatgctc ggacgccatg
      721 ttttcagcca aggcctggaa caccagcgcc tcgacgtaac gcaccagcag ctcgtcgatc
      781 accacttgcg catcgggctc gtagatgtaa tcccatgcgt gtccgccacc ctcagccttg
      841 ctttccgctg ccatcttggc ggaggaaagg ggcagcagct gttcgaccac agggtcctgc
      901 ttcatggtgc tgacaaactt gttgtagcag atgtgcactt cgctgacttc gcccttgaca
      961 taggcatcca gcagcacctt gaccggcccg atgagtttgt ccaggtgtgg acggtcgcca
     1021 aggtgggtga catgcgacac cactttggca ccgacacgat tcagaaaacc cagacccttg
     1081 cttccgatgg ccaccgcatg cgtcgttttg ccggcggtct gcgtctcgcg caacttcccc
     1141 gtcacaagac gcagcagatt ggtgttgagg ccaccgcaca agcccttgtc cgtagacacc
     1201 acaatcagac ccaccgactt ggcgtcgttg gtcttcatga aagcgtgcac gtactcgggg
     1261 ttggcttgcc ccagattagt cgcaatgtcc cggatcttcg tgctgtaagg acgggcagcg
     1321 cgcatacgct cctgcgcctt gcgcattttg gagaccgaga tcatttccat ggccttggtg
     1381 atcttcttgg tgttttccac cgatttgatc ttggtgcgta gttccttgcc tgctgccatc
     1441 agtggctcct tgtgatcagg tggaaatcaa gcaaaggttt tcttgaacgc agcaacagca
     1501 gcagtcaatt cagcctcggc atccttgtcc agtgcctggg tggattccac cttggccagc
     1561 agggctgcat gcttgtcttt gaggtacgac tgcaggccgt gctcaaacgc cagaatcttc
     1621 ttgacttcca cgtcgtccat gaagcccttg ttcacggcaa acagggatgc gctcatcagg
     1681 ctgatggact gggggctgta ctgcttctgc ttgagcagtt cggtcacgcg ggcaccgcgg
     1741 tcgagctgct tgcgggtggc ttcgtccagg tcagaggcga actgcgcgaa agcagccagt
     1801 tcacggtact gcgccaagtc gttacggata ccgccggact gacccttgac caccttggtc
     1861 tgggctgcac tacccacgcg ggacacagag ataccggcgt taatagcggg gcggatacca
     1921 gcgttgaaca acgaggtttc caggaagatc tggccgtcag tgatcgaaat cacgttggta
     1981 ggcacgaatg ctgacacgtc acccgcctgg gtttcgatga taggcagtgc ggtcaaagaa
     2041 ccggtcttgc ctttgacttc acccttggtg aaggcttcca cgtactcagc attcacgcgg
     2101 gctgcgcgct ccagcagacg gctgtggaga taaaacacgt cgccggggta ggcttcacga
     2161 cctggaggac ggcgcagcag cagggaaacc tgacgataag caacagcctg cttggacaga
     2221 tcgtcataca caatcagggc gtcctggccg cggtcacgga agtattcgcc catggtgcag
     2281 ccagagtagg ccgacacata ctgcatggca gcagattcag aagcggatgc agccacgacg
     2341 atggtgtatt ccatcgcacc agcctgctcc agagcacgca ccacgttctt gatcgaagaa
     2401 gccttctggc cgatcgcaac gtacacgcag gtcatgttct gacccttctg gttgatgatc
     2461 gcgtcaatag ccacggcagt cttgccggtc tgacggtcac caatgatcag ttcgcgctga
     2521 ccgcggccga ttggcaccat ggagtcgatg gacttcaggc cagtctgcat gggctgatcc
     2581 acggatttac gtgcaatcac gcctggcgca actttttcga tcacatccgt catcttggcg
     2641 ttgatcggac ccttgccgtc gataggctga cccagggcgt tgaccacacg gccaatcaac
     2701 tcgggaccca cggggacttc cagaatacga ccagtgcact tgacggtatc gccttcggag
     2761 atgtgctcgt attcacccag aataacggca ccaaccgagt cacgctccag attcagcgcc
     2821 agaccgaagg tgggcagacc atcgctacca gccgggaatt ccagcatttc gccttgcatc
     2881 acgtcagaga ggccgtggat gcgaacaata ccgtcggtca ccgagataac ggtaccctga
     2941 ttgcggatgt cggcgctagc agacagaccc tcaatacggc tcttgatcag ttcagaaatt
     3001 tctgcgggat tgagttgcat gactctttcc ttctttcttt ggttaggtgc tcagggtgca
     3061 ggcagagcct tacgccgtga gcgccacttt catttgttca agacgggcct tgacagaggt
     3121 gtcaagaact tcgtcaccca ccactacgcg aatgccgccg atcagctcgg actgcaattc
     3181 aacgctcaca ttgagcttgc ggccaaaacg cttctccagc atgttcgaca catcagccag
     3241 ggcagcaccc tcaatcggga aagcgctgta caccacggca tccgacgaac cgctctgcgc
     3301 attcttcaaa gcccgaaact gcgcagcaat ttccggcaat gcactcaggc gcccgttctc
     3361 gatcaccatg cgcaggaaat tcttggcatg gtcattgagc gcagccttgg caacacccgt
     3421 gatgaggtca aacacctgag cagcagtcac cttggggctg tctgcaaatt gcagcaactg
     3481 ggcattcccg gctaccgcag caagctcatc cacccaagca gcagtggcag acagatcctg
     3541 gacggatgcc ttgaacaagg catccgcgta aggtcgggca atagtggcga gttcagccat
     3601 gttgtcctct tacagctctg tcttcagacg gccaagcagg tccgcatgaa cgccagcatt
     3661 gacttccttg cggagaatct gctcagcacc cttgacagcc agcgcagcga cctgctcacg
     3721 cagcgattcg cgggccttga cggtttgctg ttcagcttca accttggcag cagcaatgat
     3781 cttgttgcct tcttcggtgg ccttggcctt ggcctcttcc acgatggctt gtgcgcggcg
     3841 ctcggcatcc gccaggcgtg cagcattctc attgcgcgac ttggccaact cgtcttccac
     3901 gcgcttgttg gcggcggaaa gctctgattt ggctttgtcg gcagcagcga ggccatccga
     3961 aattttcttg gcacgttcat ccagtgcggc tgcgatggga ggccacacga atttcatcgt
     4021 gaaccacacc aggatcgcaa atacgatcgc ttggacgaac agggttgcgt taatactcac
     4081 gtcaacacct ttctagcgtt ggactgcgaa attacttcag aacgaagggg ttggcgaaag
     4141 caaacatcat ggcgatacca acgccaatca ggaaggacgc gtcgatcaga ccggccaaca
     4201 cgaacatttt gccttgcagt tcattcatca gctcaggctg gcgagcagct gcttcgaggt
     4261 acttgctgcc catgatgccg ataccgatac aagcaccgat agcgcccagg ccaatgatca
     4321 atccagctgc cagtgcgaca aagccgagta cgtgttccat ttaaagctcc tgtggataaa
     4381 aaagttaatg gttaagggaa agggaaatca gtgcgcgtcg tgagcctgac ccacatacac
     4441 gagggtcagc atcatgaaca caaaagcctg caggcccaca atcaaaatgt ggaaaatgga
     4501 ccagacagaa cccgcaatga tatgggcgat gggcagcatg atgcccgttg tggaggctgc
     4561 cagtgcgcta cccatcaggg caatcagcat gaacaccagt tcgcccgcat acatgttgcc
     4621 gaacagtcgc atgccgtgcg agactgtttt ggcaacgaat tcgatcatct ggaacaggaa
     4681 attgatagga cccagcttgg caccaaaggg cgcgctgaac agctcatgta tccagtgacc
     4741 caagcccttg atcttgatgt tgtagtaaat gcaaaccagc agcacggaaa cgctcatacc
     4801 cagcgtcacg gagaggtccg ccgtaggcac tacacgcatg taggtgtgat gctcatcacc
     4861 acccgaaagc atcaaccaca gcttgggcag caaatccacc ggcaggaagt ccatggaatt
     4921 catcaggaaa atccagacaa acaccgtcaa ggcgagcggg gccaccattt tgcggctttg
     4981 ggcgttgtgc acaatgcctt tagcctgctc atccacaaac tccaccagca actcaaccgc
     5041 agcctgaaag cgaccgggaa cgccagcatg cgcacccttg gcggcacgcc agaggaagaa
     5101 acagccaacc acacccaaca acaccgacca gaagatcgag tcatagttgt aaaccgaaaa
     5161 gtcaatcaca cccttcatgg gctggttttg ccaatgggtc aggtggtgaa caatgtattc
     5221 accggccgtt gggccttggt gcgcggcgcc ttccgcgaca gcagcttcag cagacatgtt
     5281 tcaacaaacc cttatcaatg cttacgtttc ggatggcgaa tcaacgccag ccagtacacc
     5341 ttcatcgtga caaccagact gaccagcatg accggccaat tcagattcat caccaggcga
     5401 tgagcaacaa aaagaatgct caccgtcaac acaatcttga caaattccca cagaaagaag
     5461 cccattacgg ccgcccccgc gtttgccgaa gcaaattgcc ccgccacacc ccgcgcaaac
     5521 agcgccgccg gaatcaccac agccaacgca ccgaaggctg ccgaccacgc caccgaagtc
     5581 ttgccggaaa ttccccacgc cgccagggtc accagaaccc cgaccaaaac ctgccccaaa
     5641 acaacccacc aaggagaagt tgaaggattc tgctctcgca acgtttgcgc ctgctccgca
     5701 gtcaatggca cgaagtccga gacttcgcca tcctcatcgg cttccggggg cattgtcgtc
     5761 atatcagatt acgcccaggt tacagactgc gatttgtgca cagccagcga ttatacgtag
     5821 aaacccggtg ctccaaaaca gtcgcagcca atattgcacg cccgatctgc acataaaccg
     5881 ccccactgcg ataatttcgc gatgcacaac ctgccacgcc tgccctgtta cctcaatggc
     5941 gagtacaccg ttttgcccga ggccaaggtc agtgtcatgg atcgagggtt tatttttggc
     6001 gatggcgtgt acgaggccat tcccgtgtac gccggtcagc ccttccggtt cgcgcaccat
     6061 atggcacgcc tggaacgcag cctgcaggaa acccggattc cccagcccca cagcaccgcc
     6121 caatggcagg aaattgctct cagtttaata gctgcttacg cccattctac gggggctagc
     6181 acccaaaata ctgatcagtt tgtctacctc caggtcaccc ggggtgtcgc catgcgcgac
     6241 cacgtgatgc cgcagggaat cgcgcccacc gtatttgcca tgaccaatgc catgaagcca
     6301 cccacggcac agcagcgggc cgatggcgtg gcctgcgtga cggcggaaga cttccgctgg
     6361 gagaaagccc acatcaagag caccagtctg ctgggcgccg ttatggcccg ccagaccagc
     6421 ttcgacgcgg gcgcggtgga aaccgtcatg ttccgcagag gtttcctcag cgaagcggcg
     6481 gccagcaatg tctgggtcat caagcatggc gtcgtcatgg gcccgcccaa agaccacctg
     6541 gtgctcgaag gcatccggta cggcctgatc gaggaaatct gccaagcggc ggacattccc
     6601 ttcgtcctgc gcccggttgc aaaaagcgaa gtgctggacg ccgacgaact gctgctgtcc
     6661 tcggccacca aggaagtgct gcccatcacc accctggacg gccagccggt tggaaatggc
     6721 cggcccggcc ccatctacca ggctctgtac gaaggctacc agcgcatcaa ggcgcaatct
     6781 gccgcctctg ccacccacta accagcgagc tcccgtgtcc ctgccagaaa ttcccccatc
     6841 cgagtccctg atcacctacc cgtcgcagtt ccccatcaag atcatgggcg taagcaccga
     6901 aggctttgtg cacgccatca ccaccatcgc ccacgcgttc gaccccgcat ttgatgcctc
     6961 caccatcgaa ctgcgcgaaa gcaagggcgg caaatacctg ggtgtgacca tcaccatcac
     7021 cgccaccagc cgcgaacagc tcgatgagct gtaccgcacg ctgtcgaccc accccatggt
     7081 caaagtggtg ctgtagcgcc atggatttca aggtctccaa tctcggccgc gtggactacc
     7141 tgcccaccta cgccgcgatg caggcattta cagaccaaag agacgtgggg cccgcgtcca
     7201 ccgtgcagga ccagctatgg atttgtgagc accccccggt ctttacccag ggactggcgg
     7261 gcaaggcgga gcatattttc aacccagcca acatccccgt ggtacagacc aaccgcggcg
     7321 gccacgcgat agcaatacaa ctccagctca gcggaat
//
LOCUS       DAOSJM010000075         7254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9390_length_7254_cov_3.694124, whole genome shotgun sequence.
ACCESSION   DAOSJM010000075 DAOSJM010000000
VERSION     DAOSJM010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9390_length_7254_cov_3.694124"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PKX82_04015"
     CDS             <1..253
                     /locus_tag="PKX82_04015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOF51446.1"
                     /translation="TQGGLAAMRYTDHFGQPTETYPLNPNGSPGGLTAVTTADGRFTA
                     MMPHPERVFRNVQMSWTSLDKSEFSPWMELWRNARKWVG"
     gene            405..1043
                     /locus_tag="PKX82_04020"
     CDS             405..1043
                     /locus_tag="PKX82_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOF51447.1"
                     /translation="MSDLIQKLQWRYAAKKMNPAKSVPQEKLDRILEAIRLTATSSGL
                     QPYEVFVVTNKEVREKIKPIAWNQEQITDGSHLLVFAAWDNYTADRINMMFDLVNEQR
                     GFKNEGWENYRQMLLSMYPQRDAEVNYQHAARQAYIGLGTALIAAAEEQVDATPMEGF
                     DPKALDEILGLKALGLRSVVILPLGYRAEDGDWLVSLKKVRRAREDFITEVA"
     gene            complement(1054..1545)
                     /locus_tag="PKX82_04025"
     CDS             complement(1054..1545)
                     /locus_tag="PKX82_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOF51448.1"
                     /translation="MTSPSPTPREQDVSGLVAAIARNTEDWSLGRFLEGFRWTVLADY
                     VKPAELERGHLLIAQGEKDRTLYFLESGNVKVDMRTDAGLVHLAVLGPGTVVGEGGFF
                     ANLPRNASVSVYSDCKVWAMPIAKFAELARQHPSVALALTLALGAVLASRMLDVSKRL
                     PVT"
     gene            1798..2211
                     /locus_tag="PKX82_04030"
     CDS             1798..2211
                     /locus_tag="PKX82_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51449.1"
                     /translation="MPAFADEKPFALGVHLGTFHTTGTYNNINTGVYGRFNSWTVGGY
                     HNSIRKPSYYVAYTWEIPTPHVPLVESFAITAGGITGYESSLNDSGVLPMLVPSAAFR
                     ITPKVLLRLSILPIDGQKIGSSGAALHFSLEHPLQ"
     gene            complement(2205..4223)
                     /locus_tag="PKX82_04035"
     CDS             complement(2205..4223)
                     /locus_tag="PKX82_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOF51450.1"
                     /translation="MSLPDYQLELLAPARDADIGIEAINHGADAVYIGGPSFGARSSA
                     DNGVADIARLAQHAHRFNSRIFVTLNTILRDDELEPARKLAWQLYDAGVDALIIQDMG
                     LLEIDLPPIQLHASTQTDIRTPEKARFLQDVGLSQIVLARELTLQQIADIRAATNPEL
                     CTLEFFVHGALCVAYSGQCYISAAHTGRSANRGECSQACRLPYQVVDSQGRFVAHDKH
                     VLSMKDNNQSENLAALVDAGIRSFKIEGRYKDMGYVKNITAHYRRLLDELVENRQDSD
                     HPYARVSSGSTTFTFTPDPDQNFNREFTDYFVQGRKDDIGAFDSPKNPGQSIGYVTQV
                     GPNWVQLELNDSSLQLSNGDGLCYYDLQKELVGMAINRAEAINAARGQWRVFTKDPLS
                     ALKDLRKGVEVNRNRDVNWVRVLEKKSSDRRIGVWARLVETPDGMALTLTDEDGFTAT
                     AQTSLRREVAKDASSALDALRDNISKMGATIFSALDVQVEFSQPWFIPASVLNPLRRE
                     ALEALERTRARGFVRLERRQALEPPVPYPEDALSYLANVFNQKAHDFYARHGVKVISP
                     AYEAVEELGDVSLMITKHCVRFSLSLCPKQAKGVTGVQGQVKAEPLQLINGKEKLTLR
                     FDCKPCEMHVVGKMKTSVANQSRKELQDARSGTPLTFHKTRPKQEFGH"
     gene            complement(4273..5874)
                     /locus_tag="PKX82_04040"
     CDS             complement(4273..5874)
                     /locus_tag="PKX82_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOF51451.1"
                     /translation="MAIAGISLSDTDGQVFLRSDPSSERHPLETDHLRALLQEAGYGD
                     WVVDESALAIAVQDCNHKKEPFVVPLAQRLDASIQVVVAPDEMTAAVTLIPPQGGKAA
                     EIEDILQALHEAGVTFGIDSAALLEACQAGSVHDLPVASGAQAQDGQDADFEEMVPQA
                     SNRAPRLDANGLIDYREHSGIALVEPGAPLMRRIPATQGIEGRTIKGAVIVPRPGRDE
                     PFAAQLIGAEIDSADPNILKASVTGQPVRVPCGVMVEPVLRVPEVNLATGNIYFDGTV
                     QVDGDVNNTMKVQASGDIVVGGTVEGAQLDAGGNITIKGGVIAQARVKAGGAITARFC
                     EAASLEAGTVIALDDMALESQLRSGNQILIGTKAPQRGRLVGGTTHTMMLLKVPYLGS
                     TKTGVTQVAVGSHPELEAQYQALQERLAKEKANEDNLQKLSHHLTSIGDPKGMLDRVK
                     ASWRQAVQIWGKSLAERTELDKQLARTRSAKLEVGLSTEGMVELAFGSKKLRLRKEYK
                     SGTFSLDAEARIVFTDPSGQAYPAA"
     gene            complement(5989..6774)
                     /locus_tag="PKX82_04045"
     CDS             complement(5989..6774)
                     /locus_tag="PKX82_04045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF51452.1"
                     /translation="MKKQLLTALATAALLAALAPSAIAQNIAIVNGKPVPKARMDALA
                     QQMARAGRQIPPEMQGQLKDEVITREIFVQEAQARGYDATEDYKNQMELARQSILIRE
                     LFGDFQKKNPVTDEEAKAEYDRFAAANAGKEYRARHILVEKEDEAKAIIAQLKKGAKF
                     DAIAKKQSKDPGSGANGGDLDWATPSNYVKEFAEALTALAKGKTTETPVKTQFGYHVI
                     RLEDVRDAQLPKFEDVKPQIAQQLQQQKMTKFQDELRAKAKIE"
     gene            complement(6794..7084)
                     /locus_tag="PKX82_04050"
     CDS             complement(6794..7084)
                     /locus_tag="PKX82_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HOF51453.1"
                     /translation="MNTGPTAAQLDARLRERLQFTSLEVIDESFQHAGHAGANGSGFG
                     THFRVRITSQLFTGKSRVQRHRLVYDALQDFIDQGLHALAIEAEASPQANPI"
     gene            complement(7081..>7254)
                     /locus_tag="PKX82_04055"
     CDS             complement(7081..>7254)
                     /locus_tag="PKX82_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HOF51454.1"
                     /translation="TAFMAILNGYVVLYYSTEAWVAFKLWGYVFPLAFIIGQGIYIAP
                     HIKTENNKDSTTP"
BASE COUNT         1363 a         2136 c         2180 g         1575 t
ORIGIN      
        1 cacgcagggt ggcttggccg ccatgcgtta taccgaccac tttggccagc ccaccgaaac
       61 ctacccactc aaccccaacg gcagccccgg tggtctgaca gcggtgacca cggcggacgg
      121 ccgcttcacc gccatgatgc cgcaccccga gcgcgtgttc cgcaatgtgc agatgagttg
      181 gaccagcctc gacaagagcg aattcagccc ctggatggag ctctggcgca acgcccgcaa
      241 gtgggtgggg tagttgtcct gcctggtggt taacacatcc tgttgattta agcagcgggc
      301 gggacaaatc aggggtgcac aggccgtgat tggggagggt ggaacacgct catcggcaaa
      361 gtcgcccaga atccaccccc ttatcaatgc aatgaagtgt gtacatgtct gacctgatcc
      421 agaagctgca atggcgctac gctgccaaaa agatgaatcc cgccaagtcg gtcccccagg
      481 agaaactgga ccgcattctg gaagcgattc gcctgactgc cacgtccagc ggtttgcagc
      541 cctatgaagt atttgtggtg accaacaagg aggtgcgcga gaaaatcaag cccatcgcct
      601 ggaaccagga gcagatcacc gatggctcgc acctgctggt gtttgccgcc tgggacaact
      661 acaccgctga ccgcatcaac atgatgttcg acctggtgaa tgaacagcgt ggcttcaaga
      721 acgaaggctg ggaaaactac cgccagatgc tgctgtccat gtacccccag cgggatgccg
      781 aggtgaacta ccagcacgcc gcacgccagg cttatatcgg cctgggtacc gcgctgattg
      841 ccgcggccga agagcaggtg gacgccacgc ccatggaagg ctttgacccc aaggcactcg
      901 atgagattct gggtttgaaa gcccttggct tgcgcagcgt tgtcattttg ccgctgggct
      961 accgtgcaga agacggtgac tggctggtga gcctgaagaa ggtccgccgc gcacgcgaag
     1021 atttcatcac cgaagtggca taagccactt ccctcaggtc actggcagtc gcttgctgac
     1081 atccagcatg cgactggcca gaaccgcacc cagggccagt gtgagggcca gggccacact
     1141 ggggtgctgc cgggcgagtt ctgcaaactt cgcgatgggc atggcccata ccttgcagtc
     1201 gctgtacacc gagacagaag cgttgcgggg caggttggca aaaaaaccgc cttctcccac
     1261 cacggtgccc gggccgagca ccgccagatg gaccagtcct gcgtctgttc tcatatcgac
     1321 cttgacgttg ccggactcca gaaagtacag cgtacgatcc ttctcgccct gggcaatcag
     1381 caaatggccg cgctccagtt cagcgggttt gacatagtcg gccagtacag tccagcggaa
     1441 gccttccaga aacctgccca gggaccagtc ttccgtattg cgggcgatgg ctgcaaccag
     1501 tccactcaca tcttgttcac ggggagtggg gcttgggctt gtcatgggag atcctcctgg
     1561 gggcggggtt gttattgcgt tgctttggca caggcatagg ttacccagtt cttgccagtg
     1621 gtgtccagtg tttatgttgt accttgcagc tactccataa ccttgaggca aaatgcggaa
     1681 tcctgggtgg gctagcgcac tgaattttca ctgcctatcg gtcttactct gtaggtctac
     1741 ataaggttca gatccatgcg gaagctcgtg ataagtatcc tggcggttct acacgccatg
     1801 ccagcctttg ctgatgaaaa gccctttgcc ctgggggtac acctgggaac cttccacacc
     1861 acgggcacct acaacaacat caacaccggt gtgtatgggc gcttcaattc ctggacggtg
     1921 ggcggctacc acaacagcat acgcaaaccc tcgtactacg ttgcctatac gtgggaaatt
     1981 ccaacgcccc atgtgccgct ggtggagtct tttgcgatca cggctggcgg aatcaccggt
     2041 tatgaatcga gcctgaatga ctctggcgtg ttgcccatgc tggtgcccag tgcagcgttc
     2101 cgcatcaccc ccaaggtgct gttgcgcctg tctatcttgc cgatcgatgg gcagaagata
     2161 ggcagttccg gtgcagcgct gcactttagc ctggaacacc cgcttcagtg accaaactcc
     2221 tgcttcgggc gggtcttgtg gaaagtcagc ggtgtgcctg agcgcgcgtc ctgcagttcc
     2281 ttgcggcttt gattggccac cgaggttttc attttcccga ctacgtgcat ctcgcagggt
     2341 ttgcagtcaa aacgcagggt gagtttttcc ttgccgttga tgagttgcag gggctcggct
     2401 ttcacctgcc cctgaacgcc agtcacgccc ttggcctgtt tggggcacaa gctcagggaa
     2461 aagcgtacgc agtgcttggt gatcatcagg ctcacgtccc ccaattcttc gaccgcttcg
     2521 taggccggtg agatgacctt gacgccgtgg cgggcataga agtcatgcgc cttctggttg
     2581 aacacgttgg ccagataact gagcgcatct tcggggtaag gcaccggtgg ttcaagggcc
     2641 tgcctacgct ccagacgcac aaagccacgc gcccgagtgc gctccagggc ttccagcgcc
     2701 tcccggcgca gcgggttgag caccgatgcc ggaataaacc agggctggct gaattccacc
     2761 tgcacgtcca gtgctgaaaa gatggtggca cccatcttcg agatattgtc ccgtagcgca
     2821 tcgagcgcag aagatgcgtc cttggcaact tcacggcgca gggacgtttg tgcggtcgct
     2881 gtaaagccgt cttcatcggt gagtgtcaac gccatgccgt cgggcgtctc gaccaaccgc
     2941 gcccacacgc caatacgccg gtcactggat ttcttttcca gcacgcgcac ccagttaacg
     3001 tcacggttgc ggttgacttc cacacctttg cgcagatcct tcagcgcaga caaggggtct
     3061 ttggtaaata cccgccactg gcctctggcg gcgttgatgg cttccgcccg gttgatggcc
     3121 atgcccacca gctccttctg caggtcgtag tagcacagac cgtcgccatt gctcagctgc
     3181 aggctggaat cgttcagctc cagttgcacc cagttgggac ccacctgggt gacatagccg
     3241 atggactggc ccgggttctt ggggctgtcg aaagcgccga tgtcgtcctt gcggccctgc
     3301 acaaagtagt cggtgaactc gcggttgaag ttctggtccg ggtcgggtgt gaaggtgaag
     3361 gtggtgctgc cgctactgac gcgtgcgtac gggtggtcgg agtcctgacg gttttccacc
     3421 agctcatcga gcaatctgcg gtaatgggcg gtgatgttct tcacatagcc catgtccttg
     3481 tagcgcccct cgatcttgaa gctgcggatg cccgcgtcca ccagtgcggc cagattctca
     3541 ctctggttgt tgtccttcat ggacagcaca tgcttgtcat gcgccacaaa gcggccctgg
     3601 ctgtccacca cctggtaggg caggcggcag gcctggctgc attcgccccg attggcgctg
     3661 cgccccgtat gcgcagcact gatgtaacac tggccactgt aggccacgca cagggcgcca
     3721 tgcacaaaaa actccagcgt gcagagctcc gggttggtcg cggcgcgtat gtcggcaatc
     3781 tgctgcaggg tcagttcgcg ggcgagcacg atttgtgaca gtcccacgtc ctgcagaaag
     3841 cgcgctttct cgggagtgcg gatgtcggtc tgggtactgg cgtgcagctg gatcggaggc
     3901 aggtcgattt ccagcagccc catgtcctgg atgatgagcg catcgacgcc cgcgtcgtac
     3961 aactgccagg ccagcttgcg ggcaggctcc agttcgtcat cgcgcagaat cgtgttcagt
     4021 gtgacgaaga tgcggctgtt gaaccggtgc gcatgctgcg ccagccgggc aatatctgcc
     4081 acgccgttat cggcactgga acgggcgccg aatgaggggc cgccaatgta gacggcatcg
     4141 gcaccatggt tgatggcctc gatgccaatg tcggcatcgc gtgcgggagc cagaagctca
     4201 agctggtaat cgggcaggga catggggcct gattatccca gcgtcgcgaa acgctggccg
     4261 acgcacggtg ttttatgccg ccgggtatgc ctggccggac gggtcggtaa acacgatgcg
     4321 ggcttcggca tcgagcgaaa aagtaccgct cttgtattcc ttgcgcaggc gcagcttctt
     4381 gctgccgaac gcaagctcca ccataccttc ggtgctgagg cccacttcca gcttggcgct
     4441 gcgtgtacgc gccagttgct tgtccagctc tgtgcgctcc gccagggact tgccccagat
     4501 ctggactgcc tgacgccagg aggctttcac ccggtccagc atgcccttgg ggtcgccaat
     4561 gctggtgaga tggtgggaga gtttttgcag gttgtcctcg ttggccttct ctttggcaag
     4621 tcgttcctgc agggcctggt attgcgcttc cagctccgga tgggatccca cggcaacctg
     4681 ggtcacgcct gtcttggttg acccgaggta gggtactttg agcagcatca tggtgtgtgt
     4741 ggttccaccc accaggcgtc cacgttgcgg tgccttggtt ccaatcagga tctggttgcc
     4801 cgaacgcagc tggctttcga gcgccatgtc atccaatgcg atcacggttc cggcttccag
     4861 cgaagccgcc tcgcaaaagc gcgcagtgat ggctccgcca gccttgacgc gggcctgcgc
     4921 gatcacgccg cccttgatgg taatgttgcc accggcgtcc agctgcgcac cttctaccgt
     4981 gccgcccacc acgatgtccc cactggcctg caccttcatg gtgttattca cgtcaccatc
     5041 gacctggacg gtgccatcaa agtagatatt gccggtagcc agattgactt caggcacccg
     5101 caatacgggc tcgaccatga cgccacaagg tacccgtacc ggttgaccag tgacgctggc
     5161 cttgaggata ttggggtcgg cactgtcaat ctcggccccg atcagctgcg cagcaaaggg
     5221 ctcgtcgcgg ccagggcggg ggacaatgac ggcccccttg atggttcggc cctcgatgcc
     5281 ctgcgtggcg ggaatgcggc gcatcagggg agcacctggc tccaccaagg cgataccgct
     5341 gtgctcccgg tagtcaatca gtccgttggc atccagtcgt ggcgcgcggt tggaggcctg
     5401 gggcaccatt tcttcaaagt cagcgtcctg accatcctgc gcctgcgcac cgctggccac
     5461 cggcaggtca tggaccgatc cggcctggca ggcctccagc aaggcagccg agtcgatgcc
     5521 aaaggtgaca cctgcttcat gcagtgcttg caaaatgtct tcaatctcgg cagccttgcc
     5581 gccctgcggc gggatcaagg tgaccgcggc ggtcatttca tcgggggcta ccaccacctg
     5641 gatactggca tccagccgct gcgccagcgg caccacaaaa ggctctttct tgtggttgca
     5701 gtcctgcact gcaatggcca gggcgctttc atcgacaacc cagtctccat agcctgcttc
     5761 ctgcagcaac gcgcgcagat ggtctgtttc cagcgggtgg cgttcggacg atgggtcgct
     5821 gcgcagaaat acctgcccgt cggtatccga tagagaaatc ccggcaatcg ccatacgtca
     5881 acgcgctcct tgttggtgcg actttgtacc acggcaggta taccgatgtc acgaaaacgt
     5941 caaaaaaaac gcgacccaag gtcgcgttga tggagcaagg gaagaccgtt actcgatctt
     6001 ggccttagcg cgcaattcgt cctggaactt ggtcatcttc tgttgttgaa gctgctgtgc
     6061 aatctggggc ttgacgtcct caaatttggg cagctgcgca tcgcgaacat cttccaggcg
     6121 aatgacgtgg tagccaaact gggtcttcac cggggtttcc gtggttttgc ctttggccaa
     6181 cgccgtcagc gcttcggcaa attccttgac gtaattgctg ggtgttgccc aatccagatc
     6241 gccaccgttg gcaccggagc ctggatcctt ggactgcttc ttggcaatgg cgtcaaattt
     6301 ggcgcctttt ttcagctggg cgatgatggc cttggcttcg tcttccttct ctaccaggat
     6361 gtggcgggcg cggtattcct tgccggcatt ggccgccgca aaacggtcgt attcggcctt
     6421 ggcttcttca tcggtcaccg gattcttctt ctggaagtcg ccgaacagtt cacgaatcag
     6481 gatggactgg cgggccagct ccatctggtt tttgtagtct tcggtggcgt catatccgcg
     6541 tgcctgggct tcctgcacaa agatctcgcg ggtgatgacc tcatccttga gctgaccctg
     6601 catttcgggc gggatctggc ggccggcacg ggccatttgc tgtgccagtg cgtccatacg
     6661 ggctttggga actggcttgc cattgacgat ggcaatgttc tgcgcaatgg cagagggggc
     6721 cagcgcagcg agcagggcgg cagtggccag ggcggtcaaa agttgctttt tcattccgtt
     6781 tccttcggta ttctcaaatg gggtttgcct ggggtgaggc ttcggcctca atggccaggg
     6841 cgtgcaggcc ctggtcaatg aaatcttgca atgcatcata cacaaggcga tggcgctgaa
     6901 cgcggctctt gcctgtaaac aattgtgacg tgatgcgcac gcggaaatgg gtgccaaaac
     6961 cggagccatt ggcgccagcg tgcccggcgt gctggaagct ttcgtcaatc acttccaggc
     7021 tggtaaattg caggcgttcg cgcaggcgcg catccagttg ggcagcggtt gggccggtgt
     7081 tcatggcgta gttgaatcct tgttgttttc ggtcttgatg tggggggcaa tataaattcc
     7141 ctggccaatg atgaatgcca aggggaaaac atagccccac aacttgaacg cgacccaggc
     7201 ttcggtgctg tagtagagca ccacgtagcc gttgagaatg gccatgaatg cggt
//
LOCUS       DAOSJM010000076         7239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9406_length_7239_cov_4.428870, whole genome shotgun sequence.
ACCESSION   DAOSJM010000076 DAOSJM010000000
VERSION     DAOSJM010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9406_length_7239_cov_4.428870"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="minD"
                     /locus_tag="PKX82_04060"
     CDS             complement(<1..521)
                     /gene="minD"
                     /locus_tag="PKX82_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOF51455.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASFATGLALRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEANLNQALIKDKQCDNLFVLAASQTRDKDALTQDGV
                     EKVLNDLAAMDFEFIVCDSPAGIETGALMAMHFADEAIVVTNPEVSSVRDSDRILGML
                     ASKTKRAMEGGGA"
     gene            complement(552..1292)
                     /gene="minC"
                     /locus_tag="PKX82_04065"
     CDS             complement(552..1292)
                     /gene="minC"
                     /locus_tag="PKX82_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOF51456.1"
                     /translation="MSVSFEIKSAQLPLVALLLKTTDITALARDLNQQFGPDGESPDF
                     FDQDALVLDFSHFEAATPCEDIAHLLDAVRACRLVPVAVRGAQGPWLEAAKAAGLVEA
                     PVEIARPRAEVVHTSETVEVVKEVVREVPGPATMVVDKPLRSGQKIYARGADLVVLAM
                     VNQGAEVVADGNIHVYAPLRGKAMAGARGNTHARIFSLCLEPELISIAGVYRTSENPL
                     AKDVQGKAAQVRLSNDGQDKLLIEALKT"
     gene            1681..2628
                     /locus_tag="PKX82_04070"
     CDS             1681..2628
                     /locus_tag="PKX82_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF51457.1"
                     /translation="MKIAFRIVGVLLITCLVFAIAGVMATWAPDRSVEQLGSRWAQAP
                     SRFMEVSGMQVHLRDEGPRDDPLPIVLLHGTSASLHTWDGWTQELKHHRRVIRFDLPG
                     FGLTGPSPSNNYSIEAYVRFVTAVLDALGVKSFVLAGNSLGGQIAWSTASVLPQRVSR
                     LILVDAAGYPSQPVSVPLAFQVARLPGIRTLMEYVLPRGVVMQSVRNVYGDPSKVTPE
                     LVDRYYELSLRAGNRAALAHRFAQIGSGDVNQIKALKLPTLIVWGGKDRLIPPDNAKR
                     FEADIVGSRLVVFDELGHVPHEEDVQSTLQPVKTFLNIQ"
     gene            2694..4247
                     /locus_tag="PKX82_04075"
     CDS             2694..4247
                     /locus_tag="PKX82_04075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF51458.1"
                     /translation="MSEKKPFEVARETLKQLTTRKLAPTPLNYQNIYNEIAGIPQVPQ
                     FPMDTLRDIAKALPTKTPGQQKHRGLLEYAIDRMNWEGVKTALVAYGGFVPASTSDAQ
                     PSGSAPLSAVSDVAASAPALTAKFMGQIGRLIEYALPALGTDDERFREQTTGLLKALK
                     DPASDVATLKLMLVNYSHRLSFAAEDQAEIKTVLLKLLHLVFENIGELSLEEQWLKGQ
                     MDALMTASAPPLSLRRLDDVERRLKDVIFKQKEAKERTVAAQNEMREMLASFIDRLSE
                     MTQSTGTFQTKLEESARLIEQAKSMKDIAPVLKDIVGATRHMASDSQTARDELSAMRE
                     RASRTEEELTKLHQELDRVSSEARHDPLTGALNRKGLDEAIDRELSTVRRKETPLCMA
                     LLDIDNFKKLNDAMGHATGDSALNHLATVARESMRPQDTLARFGGEEFVILLPDTPLD
                     RGIEAMTRLQRELTKRFFLAGNEKILITFSAGVAQLAEGESGMDAIRRADQAMYVAKR
                     AGKNRVLGA"
     gene            4262..4840
                     /locus_tag="PKX82_04080"
     CDS             4262..4840
                     /locus_tag="PKX82_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HOF51459.1"
                     /translation="MTYLILGLVVFLGVHSVRIVADGWRTRTLARVGEGTFKGVYSVL
                     SLAGFALMVWGFGVARETPLQLWSPPTGLRHAASLLTLIAFVLLAAAYVPRNAIKARL
                     HHPMVLGVKSWALAHLLSTGAVAHLVLFGSFLLWGVLDYIASRRRDRTLGTRYPAGTL
                     AGTGMTLVVGVLAWAAFAFWLHGVLIGIRPFG"
     gene            complement(4856..5065)
                     /locus_tag="PKX82_04085"
     CDS             complement(4856..5065)
                     /locus_tag="PKX82_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HOF51460.1"
                     /translation="MFGYTEEQIAGFGLTFGVGGFMLYMLFIIGHLAWESKAGKFGTF
                     VIFLGLAFGMVGFVAKSVIQWYMER"
     gene            complement(5065..5916)
                     /locus_tag="PKX82_04090"
     CDS             complement(5065..5916)
                     /locus_tag="PKX82_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOF51461.1"
                     /translation="MSTPRTPPSLPAVRTIGLMLPLSWLVLAWRDMARSGWISFAHGL
                     ALALAGAAIVAIAHQRFWLLAGALSGFLVVAPVLATSLYALSRALERGEKADLSVVLK
                     TWLNWQNNHFNKWGNDYWCMVQFGALLALAATGWVLTSAALITLLAPVPIHTPLDFVH
                     HVILAKNGWLFELWLMMGGIIAAPMFASSVVAMPLLLDRRVTLMQAILTSWQTVLFNP
                     VPMAFWAALIMLFTLLGLGALLIGLVLVIPMLGHGSWHAYRALVDTSTLEEREPVGVK
                     PLSGGAV"
     gene            5977..6342
                     /locus_tag="PKX82_04095"
     CDS             5977..6342
                     /locus_tag="PKX82_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOF51462.1"
                     /translation="MTRPILSEEHIHPAIRERVASHHRSIVDEVQAAVAANRVVVVGM
                     GMNPMPKKARAALDAAGVPYKYLEYGNYFNTWRRRNALKMWTGWPTFPMVFVQGTLVG
                     GAADLEALIASGELKKLLA"
     gene            complement(6349..6888)
                     /locus_tag="PKX82_04100"
     CDS             complement(6349..6888)
                     /locus_tag="PKX82_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOF51463.1"
                     /translation="MKPVFKSLAVAGLLAAAGFATYAQGPGGMGMMDGDGHGRMGRMD
                     PAKMEQMVARRAADLKAKLKITPAQESAWTSFTAAMKPPANWQEKRMDRAELDKLTTP
                     ERIDKMRTLRAERTAEMNAAMDKRDEATKTFYAALNADQKKVFDAEHARMGQHAARGG
                     HHGPKGPRGGASAPVAPKQ"
     gene            7032..>7239
                     /gene="rdgC"
                     /locus_tag="PKX82_04105"
     CDS             7032..>7239
                     /gene="rdgC"
                     /locus_tag="PKX82_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOF51464.1"
                     /translation="MFKNVMMYRIVSAWEQTVAKLEAALEENRFVECGASQEKAVGWT
                     APRGDEHGPLVEVVAGQWILKLMVE"
BASE COUNT         1546 a         2012 c         2184 g         1497 t
ORIGIN      
        1 atagccccgc ccccctccat ggcacgcttg gtcttgctgg ccagcatgcc cagaatgcgg
       61 tccgaatcgc gcaccgaaga cacctcgggg ttggttacca caatcgcttc atcggcaaag
      121 tgcatggcca tcagggcccc ggtttcaata ccagcgggcg agtcgcagac gatgaactca
      181 aagtccatgg cagccaggtc attcagcact ttttccacgc catcctgggt cagtgcatcc
      241 ttgtcacggg tttgcgaagc ggccagcaca aacaggttgt cgcactgctt gtccttgatg
      301 agggcctggt tcagattggc ttcgccctga atcacattga tcaggtcata caccacacgg
      361 cgctcgcaac ccatgatcag gtccaggttg cgcaggccca cgtcaaagtc gatgacggca
      421 gtcttgtgtc cgcgcaaggc cagaccggtt gcaaaactgg cactggtcgt ggtcttgcca
      481 acgccgcctt tgccagaagt caccacaatg atttttgcca tgaagaggga gtctttcaaa
      541 aaatagataa atcaggtctt gagcgcttca atcagcagtt tgtcctgccc atcgttgctc
      601 aaacggacct gggccgcttt gccctggaca tctttggcca gggggttctc gctggtgcgg
      661 tacacaccgg cgatggaaat cagttcaggc tccaggcaca gcgaaaaaat gcgcgcatgg
      721 gtattgccac gcgcaccggc catggctttg ccacgcaagg gtgcgtacac atggatgttg
      781 ccgtctgcca ccacttcagc accctggttg accatggcca gcacgaccag atcggcaccg
      841 cgtgcgtaga ttttctgacc ggagcgcaag ggcttgtcga ccaccatggt ggcaggaccg
      901 ggcacttcgc gcaccacctc tttcacaact tccaccgttt cggaggtgtg taccacctcg
      961 gcacgcggcc gggcaatttc tacaggcgct tccaccagcc cggcagcttt tgcagcttcc
     1021 agccatgggc cctgtgcacc gcgcaccgcc acaggcacca accgacaggc tcgcaccgca
     1081 tccagcaggt gggctatgtc ctcgcaggga gtagccgctt caaaatgtga aaaatcgaga
     1141 accagggcat cctggtcgaa aaaatctgga ctttcaccgt cagggccaaa ctgctggttc
     1201 aaatcgcggg ccagggcagt gatatcggtc gttttgagaa gcagtgccac cagcggaagt
     1261 tgtgcacttt tgatctcaaa cgaaacagac atgggggatg ggaaccggtg ggtgggaaga
     1321 caaacttcga agggcaaatc ttacttgatt gctctgcaaa taccggggtg ggacctgaag
     1381 gtttatgaag gtatttttta cggggatagt tttgtctgta ttactttctt tttctattta
     1441 aaacatagta gtagtagata gagcaaagtc actttgtgtg gacaagggta tttttccttt
     1501 gaaaatcaag tacttggatc gtgtgaaccc atgtgggtga cagcgctttt ggtttgtacc
     1561 cggaaactgg ataactcttt cattttgtat cgtcctgtgg ataaccggtg acttgtaccg
     1621 aaactatgca caggcttatt ctttgacata atggaaaaag acaggtgtag agtgggcacc
     1681 atgaaaattg cttttcgcat tgtgggtgtg cttctgatca cctgcctcgt attcgccatt
     1741 gctggcgtaa tggcaacctg ggcccctgac cgttccgtag aacaactggg cagccggtgg
     1801 gcacaagcgc catcccggtt catggaagtc agcggtatgc aagtgcattt gcgggacgaa
     1861 gggccacggg atgacccgct gcctattgtg ctgctgcatg gaacctctgc gagtctgcac
     1921 acctgggatg gatggacgca ggagttgaag caccaccgtc gggtgatccg gtttgacttg
     1981 cctggatttg gtctgacggg accgagtcca tctaacaact attccattga agcctatgtc
     2041 cgatttgtca cagcggtgct ggatgctttg ggtgtcaagt cgtttgtgct tgccggaaac
     2101 tccctgggag ggcagattgc ctggtccacg gcatcggtcc tgccgcaacg ggtgagccgg
     2161 ttgattctgg tggatgcagc agggtatccg tcccaaccgg tgtcggtacc gctggccttt
     2221 caggttgcac ggttgccggg tatccgcaca ctgatggaat atgtgctgcc gcggggtgtg
     2281 gtgatgcaaa gtgtgcgcaa tgtgtatggg gatccatcca aagtgacacc cgaattggtg
     2341 gatcggtact acgaactgtc gttgcgcgca ggcaaccggg cagcgctggc acaccgcttt
     2401 gcacagattg gcagcgggga tgtgaaccag atcaaggcgc tgaagctgcc caccctgatt
     2461 gtgtggggtg ggaaagacag gctgattccg ccagataacg caaaacgctt tgaagctgat
     2521 atcgtcggtt cgcgtctggt ggtatttgac gagttagggc acgttcccca tgaagaggat
     2581 gtccaaagca ccttgcaacc ggtcaaaact ttcctgaata ttcaataaat agggctatag
     2641 cccccgtaaa tagtgtctat aaagctatta attttatagc aaatggtacc agtatgagtg
     2701 aaaagaaacc ctttgaagtg gcgcgggaaa cgctcaagca attgaccacg cgcaagcttg
     2761 caccgacgcc actgaactac cagaatatct acaacgaaat tgccggtatc ccccaggtgc
     2821 cgcagtttcc gatggatacg ctacgggaca ttgccaaagc actgcccacc aagacgccag
     2881 gccagcaaaa gcaccgcgga ttgctggaat atgccatcga ccggatgaac tgggaaggtg
     2941 tgaaaacggc actggtagcg tatggtggtt ttgttcccgc cagtacatcc gatgcacagc
     3001 cgtcgggcag tgcgccgctg tctgcagtat ctgatgttgc cgccagtgcg ccggcgctga
     3061 ccgcgaagtt catgggacaa attggccgcc tgattgaata tgcactgccg gcgctcggta
     3121 ccgacgatga acggtttcgt gagcagacga caggtttgct caaagccctc aaagacccgg
     3181 cttcggatgt cgccacactc aaactgatgc tggtgaatta cagccaccgg ctgtcttttg
     3241 ccgccgaaga ccaggcggaa atcaagactg tactgctcaa acttttgcat ctggtgtttg
     3301 aaaacattgg cgaactcagt ctggaagagc agtggctcaa agggcagatg gatgcgctga
     3361 tgacagcttc tgcaccaccg ctgtccctgc gccgtctgga cgatgtggaa cgccgtctga
     3421 aagacgtgat cttcaagcaa aaggaagcca aggaacgcac ggttgcagcc cagaacgaga
     3481 tgcgcgagat gctggcgtcg tttatcgatc gcctgtcaga aatgacgcag tccaccggaa
     3541 ctttccaaac caagctggaa gaaagtgcac ggctgattga gcaggccaag tcgatgaaag
     3601 atattgcgcc agtgctcaaa gacattgtgg gcgcaacacg ccatatggcc agcgacagcc
     3661 aaacggcgcg ggacgaactt agcgccatgc gtgaacgtgc cagcaggaca gaagaagaat
     3721 tgaccaagct gcaccaggaa ctcgaccgtg tcagttccga agcccgccat gaccccctga
     3781 ccggcgcact caaccgcaag ggtctggatg aagccatcga ccgggagctc tccaccgtgc
     3841 ggcgcaagga aacacccctg tgcatggcct tgctggacat cgacaacttc aagaaactca
     3901 acgatgcgat gggccacgcc accggcgaca gtgcgctcaa ccacctcgcc accgtggccc
     3961 gtgagagcat gcggccccag gacacgctgg cgcgtttcgg tggcgaagag ttcgtcatcc
     4021 tgctgcctga cacaccgctg gaccggggca ttgaagccat gacacggctg caacgggaat
     4081 tgaccaaacg cttcttcctg gcgggcaacg aaaaaatcct gatcaccttc agtgctggcg
     4141 tggcacagtt ggcagaaggg gagtcgggca tggatgccat ccgtcgtgca gaccaggcca
     4201 tgtacgtggc caagcgggcg ggtaaaaacc gggtgcttgg tgcctgacag ggaaattgca
     4261 catgacgtat ctgattctgg gtctggtggt gtttttgggg gtgcactcgg tgcgcatcgt
     4321 ggccgacggc tggcgcacac gtacgctggc acgtgtgggg gagggcacct tcaagggcgt
     4381 ttattccgtg ctgtcgctgg cgggttttgc cctgatggtc tggggttttg gtgtggcgcg
     4441 cgagacgcca ctgcagctgt ggagcccacc caccggcttg cgccacgcag catccctgct
     4501 gacattgatt gcgtttgtgt tgctggcggc ggcctacgtg ccgcgcaatg cgatcaaggc
     4561 ccggttgcac caccccatgg tgctgggcgt gaaaagctgg gcattggccc atctgctgtc
     4621 taccggtgct gtggcgcacc tggtgctgtt tggctccttt ttgctgtggg gcgtgctcga
     4681 ctacattgca tcgcggcgcc gcgaccgtac gctcggtacg cggtaccctg caggcaccct
     4741 ggcgggcacc ggcatgacgt tggtggtggg tgtgctggcc tgggctgcgt ttgccttctg
     4801 gttgcacggc gtcctgatag gtatccggcc tttcggttga acctgcaagg cagcgctagc
     4861 gctccatgta ccactggatg acggacttgg cgacaaagcc caccatgcca aatgccagtc
     4921 ccagaaaaat cacaaaggtg ccgaacttgc cggccttgga ttcccatgcc aggtggccga
     4981 tgatgaacag catgtacagc atgaagccgc ccacgccaaa ggtcaggccg aaaccagcaa
     5041 tctgttcttc cgtgtagccg aacatcagac agcccccccg gaaagaggct tgacgcccac
     5101 gggttcgcgt tcttccagtg tgctggtatc gaccagtgcc cggtaggcat gccagctgcc
     5161 atggcccagc atggggatga ccagcaccag tccgatgagc agggcgccca ggcccagcag
     5221 ggtgaacagc atgatcagcg ctgcccagaa cgccatggga accgggttga agagcactgt
     5281 ctgccagctt gtcaggatgg cctgcatcag ggtgacacgg cggtccagca gcagcggcat
     5341 ggcgaccacg ctggaagcaa acatgggcgc tgcgatgatg ccgcccatca tcagccacaa
     5401 ctcgaacaac cagccattct tggccagaat gacatggtgg acaaaatcca gcggcgtgtg
     5461 gatgggcact ggggccagca gggtaatcag tgcggccgat gtcaacaccc agccggtggc
     5521 agccagggcc agcagcgcac cgaactgcac catgcaccag tagtcattgc cccatttgtt
     5581 gaaatggttg ttctgccagt tcagccaggt cttgagcacc accgagaggt cagccttttc
     5641 gccgcgctcc agggcgcggc tcagggcata caggctggtg gccagtaccg gggccaccac
     5701 cagaaaaccc gacagggcac ctgccaataa ccagaaacgc tggtgggcaa tcgccacaat
     5761 ggccgcaccc gccagggcca gagccagtcc gtgggcaaag ctgatccagc ccgagcgggc
     5821 catgtcgcgc caggccagta ccagccacga aaggggcagc atcagcccga tagtgcgcac
     5881 cgcaggcagt gaaggaggag ttcggggtgt agacatggcg ccatgttact ggagccctgc
     5941 gcataatggc cctccattac cttccggagt tgtttgatga cacgccccat cctgagtgaa
     6001 gaacatatcc accctgccat ccgtgaacgc gtggcaagcc accaccgcag catcgtggac
     6061 gaagtgcagg ccgcagtggc ggccaaccgg gtggtggtgg tgggcatggg catgaatccg
     6121 atgcccaaga aagcccgcgc tgcgctggac gcggcgggcg ttccctacaa atacctggag
     6181 tacggcaatt acttcaacac ctggcgccgc cgcaacgcac tgaagatgtg gaccggctgg
     6241 cccacatttc cgatggtatt tgtacagggc accctggtcg gtggtgcagc cgatctggaa
     6301 gccctgattg ccagcggcga gctgaaaaaa ctgctggcct gacccatatt actgcttggg
     6361 agcgacgggt gcacttgcac cgccacgggg ccccttgggt ccgtggtggc cgccgcgcgc
     6421 tgcgtgttgc cccatgcggg catgctcggc gtcaaacact tttttctggt cagcattgag
     6481 cgcggcgtag aaggtcttgg tggcctcgtc acgcttgtcc atggcggcat tcatctccgc
     6541 agtgcgctcg gcacgcaggg tacgcatttt gtcgatgcgc tctggcgtgg tgagtttgtc
     6601 cagttccgca cggtccatgc gcttttcctg ccagttggcc ggcggcttca tggcggcggt
     6661 gaatgacgtc caggcacttt cctgggctgg ggtgatcttg agtttggcct tgaggtccgc
     6721 ggcgcgcctg gcgaccatct gttccatttt ggcggggtcc atgcggccca tgcggccgtg
     6781 gccatcgccg tccatcatgc ccatgccgcc aggcccctgg gcataggtgg caaaaccagc
     6841 ggcggccagc aggccggcaa cggcgagaga tttaaatacg ggtttcatgg tgacttcctt
     6901 tgaagttagg ctgtagcgga aatcgctgtc aggctggaag caagtgtgcg ctggccatgt
     6961 atctgccgta ttgttttgcg ataaggcggt gtaaagatgc gtgaaaatag ccgctgtaac
     7021 aaagggcgtg tgtgtttaaa aatgtgatga tgtaccgcat cgtgtctgca tgggagcaga
     7081 ccgttgcgaa gctggaagca gcactggaag aaaaccgttt tgtcgagtgc ggtgcctcgc
     7141 aggaaaaggc agtcggttgg actgctcccc gtggtgacga acatggcccc ctggtggaag
     7201 tggttgccgg gcaatggatt ctcaagctca tggtggagg
//
LOCUS       DAOSJM010000077         7121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9580_length_7121_cov_4.136711, whole genome shotgun sequence.
ACCESSION   DAOSJM010000077 DAOSJM010000000
VERSION     DAOSJM010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9580_length_7121_cov_4.136711"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PKX82_04110"
     CDS             <1..850
                     /locus_tag="PKX82_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HOF51465.1"
                     /translation="DAWMAYGLQDVVNQRRMIDESPAGEDFKQVMRGKLDALLGLAEA
                     TDCRRMRLLGYFGEKSEPCGNCDNCLNPPAVWDGTDAARKLLSTIYRIQQMSGVSFGA
                     GHLMDIVRGKPTEKVTQYGHETLSTFGIGSNFTELQLRGVLRQLIAMGALAVQGGEFS
                     TLQLTAESRGVLKGEVAVRLRESVSAPAERKSKRSTRLGAMVKAPIALDSDALMRYSA
                     LKAWRAEVAKEHNLPAYVIFHDATLAAIAQKAPQSMDDLQGISGMGTKKLEAYAHEVL
                     RVVGQV"
     gene            complement(869..1162)
                     /locus_tag="PKX82_04115"
     CDS             complement(869..1162)
                     /locus_tag="PKX82_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51466.1"
                     /translation="MHKEVIQVPEKGTYNWQLFNYWSDEFRQTHADHSAYIALNFAGA
                     LDACRSKLHALVQRHKQAGNSGYGRERHARAIERLQNTMTGAAMSYESCFRRT"
     gene            1400..2437
                     /locus_tag="PKX82_04120"
     CDS             1400..2437
                     /locus_tag="PKX82_04120"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HOF51467.1"
                     /translation="MQTEITQTLDLLDARGHISREGWARQPFWRYDRNAIQAPWWRIK
                     EWDYYSVLSPDQKFGLTLTMSDLGYAGMFAICFLDFERGSFHQMDTLSVLPRGKTGFG
                     ADSDQGCVRFGDKKLQMEFRYAQGERALTFSAPDLENAQGDRGIQGCISLAQPPSLES
                     INIATSWMENRQAFYYNRKINCMPAGGGFTIGDRRYTYAPERDFGALDWGRGNWTYKN
                     RWYWGSASGHTGGHSLGWNIGYGFSDRSPASENTLFVDGVAHKLAEVTFHMDESDYTR
                     PWRFSSSDGRFEMQFTPIVDRDANINLGLIRSVQHQVFGYFSGTVILDDGTALAVDQF
                     LGFAEDVLNWW"
     gene            complement(2461..4785)
                     /locus_tag="PKX82_04125"
     CDS             complement(2461..4785)
                     /locus_tag="PKX82_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51468.1"
                     /translation="MAKRSLFQPVDGHLISSPGLMEAPVLDLMCSHFVLTLAARQGAA
                     FNVRRDFSGVVGLAGRHLVWPVSVLVRLREFLARRCNANELWRGHEALDAPEFLARHG
                     VWRGPYDEATLFFFLDDYAKDQPKDLISVLSVTADALGHALKKQSTLVEKNINALAGL
                     LQLNKAERALLLYGTLARYQRDMRSILVEFKVNNASEAFAAIAEVAGVRAQDLGEALR
                     AGSRLERIGLVENLISEHNITDLADLMKVSEKLPPVLMRHYRDQAELMAVFTRLSSKS
                     ALTQDDFSFVAEDVVVLCNLLTQAVARKEAGINVLLYGPPGTGKTELAKVVAHSAGLE
                     LFEVEYADRDGNSLSGRDRYRSLQIAQVFLKGSAQAALLFDEVEDVFPPISSEAAHLI
                     ARADASAAPASASVSGKAWVNQILESNAVPTIWVTNRIEQIDPAFRRRFAYHLELKSP
                     PPGAREGVVRKTLEGVDVSDAFVARLTSRKGLTPAQIRTAVRFAALACPVDGGGPVPA
                     MEGLIERQLRNADMALGNRPAAASPDAVTQYDLALLNVHSRFEIPRMVQALQARGHGT
                     LCFYGPPGTGKTALAQHIAASLAQPLIVRQASDLMSKYVGETEQNMAAMFRDAESEGA
                     VLLLDEADSFLQDRRGAQRSYEVTEVNEMLQGMERFHGIFICTTNLMDRVDQAALRRF
                     TFKIQFKPLTRAQRETFFVTEACAGDVTALTSELAGRLGALDQLCPGDFAAVKRQMHI
                     LDTQLEPLDFLEQLEAEHRIKPEVRDARGMGFLQ"
     gene            4894..6093
                     /locus_tag="PKX82_04130"
     CDS             4894..6093
                     /locus_tag="PKX82_04130"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HOF51469.1"
                     /translation="MLAYNDILLAAQRLTGHVLHTPCVASKTLSDITGAQVFLKFENL
                     QFTASFKERGACNKLSQLTPEQSARGVIAMSAGNHAQGVAYHAQRLGLRSVIVMPRFT
                     PGVKVERTRGFGAEVVLHGDTLDEARSHALELATAQHLTFVHPYDDESIIAGQGTVAL
                     EMLEQVADLDCLVIAVGGGGLIAGMATVAKALRPGMEVVGVQTARFPAMVNAIKGTHY
                     PQGASTIAEGIAVGTPGRLTQDIIRERVDDLVLVEEGDIEQALVMLLEVEKTLVEGAG
                     AAGLAALLKYPERFQGKRVGLVLCGGNIDPLLLAAIIERGMVRAGRLARIRVSARDIP
                     GSLARITATVAEAGANIDEVHHQRAFTTLSAQNVEIELVIQTRGHAHVADVLEVLRSA
                     GFDAQLV"
     gene            6341..>7121
                     /locus_tag="PKX82_04135"
     CDS             6341..>7121
                     /locus_tag="PKX82_04135"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906657.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HOF51470.1"
                     /translation="MNSPVMQGININAPSYVKNAKLIAWVAEMAALTKPASIYWCDGS
                     DEEYQRLCQQLVDGGTFKKLNPAKRPNSFLACSDPSDVARVEDRTYICSEKKENAGPT
                     NNWMAPADMRALLQTGKDGEKALFDGCMAGRTMYVVPFSMGPLGSHIAHVGIELSDSA
                     YVAVNQKIMTRMGKAVYDVIGTDGAFVPCVHTVGAPLAAGQADVKWPCNKTKYIVHYP
                     ETREIWSYGSGYGGNALLGKKCYALRIASAMARDEGWMAEHM"
BASE COUNT         1421 a         2217 c         2138 g         1345 t
ORIGIN      
        1 cgatgcctgg atggcctacg gcctgcagga cgtggtgaac cagcgccgca tgattgacga
       61 aagcccggcg ggcgaagact tcaagcaggt gatgcgtggc aagctcgacg ccctgctggg
      121 tctggccgag gcaacggatt gccggcggat gcggctattg ggctactttg gcgaaaagag
      181 cgagccctgt ggcaactgtg acaactgcct gaacccacct gcggtatggg acggcaccga
      241 tgccgcgcgc aagctgctgt ccactatcta ccgcatccag cagatgagcg gcgtcagctt
      301 cggcgccggc catttgatgg acatcgtacg cggcaaaccc actgaaaaag tcacccaata
      361 cgggcacgaa accctgagca cctttggcat cgggtccaat ttcaccgaat tgcagctacg
      421 cggcgtactg cgccagctca tcgcgatggg ggcgctggcg gttcaaggtg gggaattcag
      481 tacgctgcaa ttgaccgccg agtcgcgtgg ggtgctgaaa ggggaagtgg cagtgcggtt
      541 gcgggagtcg gtgtcggcac cggcggagcg caagtccaaa cgcagtacgc gcctgggcgc
      601 catggtgaag gcgccgattg cgctggacag cgatgccctg atgcgctact cggccctgaa
      661 agcctggcgg gccgaggtgg ccaaggagca caacctgccc gcctacgtga tctttcacga
      721 tgccactttg gccgccattg cccaaaaggc accccagtcg atggacgact tgcaggggat
      781 cagcgggatg ggaacgaaga aactggaagc ctacgcgcat gaggtgctgc gcgtagtggg
      841 gcaggtctag gctgccccgt agtcgccgtc aggtacggcg aaagcagctt tcatacgaca
      901 tcgcggcacc cgtcatggtg ttctgcagcc gttcgatggc gcgggcatgg cgctcacgac
      961 catagccact gttgcccgcc tgcttgtggc gttgcaccag cgcatgcagc ttggaccggc
     1021 aagcatccaa cgcacctgca aaattcagcg cgatgtacgc ggaatggtct gcatgggtct
     1081 ggcggaactc gtcagaccag tagttgaaca actgccagtt gtaggtgcct ttttcgggaa
     1141 cctgaatcac ttctttgtgc atatgtgctg tgcaaaggtg gaaccatgga tgcaattgcg
     1201 atcaccagtc tttcttccaa cgatccggct cttttttgca tccgccggag aatatgacgc
     1261 gccagcaggc gccccggttg acaggcgaca ccacaaatta ctgaggcgtt acgagggttt
     1321 tccctgaaac ggggctggca aagcgtggtt tggagcgcga aacaccccca tttccccatc
     1381 caaacggtaa gctttccgca tgcaaaccga aatcacccaa acgctggacc tgctcgatgc
     1441 ccgcggccat atcagccggg aaggctgggc gcgccaaccc ttctggcgct atgaccgcaa
     1501 tgccatccag gcgccctggt ggcgcatcaa ggaatgggat tactacagcg tcctgtcgcc
     1561 cgaccagaag tttggcctga cgctgaccat gagcgacctg ggctacgccg gcatgtttgc
     1621 catctgcttt ctcgactttg agcggggcag ctttcaccag atggatacct tgagcgtcct
     1681 gcccagaggg aaaacgggat ttggcgccga cagcgaccag ggttgcgtgc ggtttggtga
     1741 caaaaagctt caaatggagt ttcgatatgc acaaggtgaa cgcgcactta cgttttcagc
     1801 acctgattta gagaatgcac agggcgaccg cggcattcag ggctgcatct ccctggccca
     1861 accgccctcg ctggaaagca tcaacatcgc cacctcctgg atggagaacc gccaggcgtt
     1921 ctattacaac cgcaagatca actgcatgcc ggccggcgga ggcttcacca tcggagaccg
     1981 ccgctacacc tatgcgcccg agcgtgactt tggcgcgctg gattggggcc gcggaaactg
     2041 gacctacaaa aaccgctggt actggggttc tgcctcaggc cacactggtg gtcacagcct
     2101 gggctggaac attggctacg gattctcgga ccgcagtccg gccagcgaga acaccctgtt
     2161 tgtcgacgga gtggcgcaca agcttgcaga agtcaccttt catatggacg agagcgacta
     2221 cacccggcct tggcgcttca gcagctctga cggccggttt gaaatgcagt tcacccccat
     2281 cgtggaccgc gacgcgaata tcaacctggg cctgatccgg tcggtgcaac accaggtttt
     2341 tggctatttc agcggcaccg tcatcctcga cgacggaacc gcccttgccg tagatcaatt
     2401 tctcgggttt gccgaagacg tgctgaactg gtggtaaatc gcgggggcga ttcgcttccg
     2461 ctattgcagg aaccccatac cgcgcgcgtc gcgcacttcg ggcttgatac ggtgctcggc
     2521 ctctagctgc tccagaaaat ccaggggctc cagctgggtg tccagtatgt gcatctggcg
     2581 tttcactgca gcaaaatcgc caggacagag ctggtccagc gcccccaacc ggcccgccag
     2641 ctccgaggtg agtgctgtta catcacccgc acaggcctcg gtgacaaaga acgtttcccg
     2701 ctgtgcccgg gtcagtggtt tgaactggat tttgaaagtg aagcgccgca atgcggcctg
     2761 gtcaacgcgg tccatcaaat tggtagtgca gataaagatg ccgtggaaac gctccatgcc
     2821 ctgcagcatt tcattgactt cggtcacctc ataactgcgt tgcgcacccc gtcggtcctg
     2881 cagaaaactg tccgcttcgt ccagcagcag gaccgcgcct tcagactccg catcccgaaa
     2941 catggccgcc atgttctgct cggtctcccc cacgtatttg ctcatcaggt cactggcctg
     3001 ccgcacgatc agcggctggg ccagtgacgc cgcaatgtgc tgtgccagcg ccgtcttgcc
     3061 ggttcccggt gggccataga agcacaaagt gccatggccc cgcgcctgca gagcctgcac
     3121 catccggggg atctcaaacc ggctgtgcac gttgagcagc gccaggtcgt actgggtgac
     3181 ggcatcggga ctggcagcgg cgggccggtt gcccaaggcc atgtcggcat tcctgagttg
     3241 ccgctcgatc agcccttcca tggctggcac aggtccgccg ccgtccaccg gacaagccag
     3301 tgcggcaaac cgcacagccg tgcgtatttg cgcgggggtc agccccttgc ggctggtcag
     3361 gcgtgcaaca aaggcgtcgg atacatccac cccttccaga gtcttgcgca ccacgccttc
     3421 acgggcgccc ggggggggag atttgagttc caggtggtag gcaaagcggc ggcggaacgc
     3481 cggatcgatc tgctcgatgc ggttggtcac ccagatggtg ggaaccgcat tgctttccag
     3541 aatctggttg acccacgcct tgccgctgac gctggcactc gcgggggctg cgctcgcatc
     3601 ggcccgcgca atcaggtggg cggcctcgct ggaaatcggt ggaaacacgt cttccacctc
     3661 gtcaaacagc agcgccgcct gcgcactccc cttgagaaaa acctgggcaa tttgcaggga
     3721 gcggtaccgg tcgcgaccgc tgagcgaatt gccatcgcgg tccgcatact ccacctcaaa
     3781 cagctccagc cccgcactgt gggccacaac cttggccagc tctgtcttgc cagtgcctgg
     3841 tggcccatac agcaacacat tgatgcccgc ctccttgcgg gccaccgcct gggtcagcaa
     3901 gttgcacaac accacgacgt cttccgccac gaaggaaaag tcatcctggg tcagcgcgct
     3961 cttgctcgat aaacgggtga acaccgccat cagttcggct tggtcgcggt agtgccgcat
     4021 cagcaccggt ggcagcttct cgctgacctt catcaggtcg gccaggtccg tgatgttgtg
     4081 ctcggatatc aggttttcca ccagcccgat gcgctccagg cgcgaccctg cacgcagcgc
     4141 ctcccccaga tcctgggccc gcacgcccgc cacctcggcg atggcagcga aggcttcgga
     4201 cgcattgttg actttgaatt ccacgaggat gctgcgcatg tcgcgctggt agcgtgccag
     4261 tgttccgtac agcaacaagg cccgttcggc cttgttgagc tgcagcagac cggccagagc
     4321 gttgatgttc ttctccacca gtgtggactg tttcttgagc gcatggccca gcgcatcggc
     4381 cgtcaccgac agtaccgaaa tcaggtcctt cggctggtct ttggcgtaat cgtccagaaa
     4441 gaaaaacagg gtggcttcgt cgtagggacc acgccagaca ccgtgacgtg ccaaaaattc
     4501 cggcgcatcc agtgcctcgt ggccgcgcca cagctcgttg gcgttgcagc gccttgccag
     4561 gaattcccgc aagcgcacca gaaccgatac cggccagacc agatgccgac ccgctagccc
     4621 caccacgccg ctgaaatcgc gccgcacatt gaaggccgcg ccctggcgcg ctgccagggt
     4681 gagcacaaaa tgcgagcaca tcaagtccag caccggggct tccatcaggc ccggagaaga
     4741 aatcaggtgc ccgtcaacgg gctggaacag cgaacgcttg gccatccaac gcctccagca
     4801 caagcttgtg cgctttcacc ataacgcagc ttgatcccgg tgggaagtat cttggcaaga
     4861 ttttttttgc cgaattcccg ccacaatgcg gccatgcttg cttacaacga catcctcctg
     4921 gccgcacaac ggctgacggg ccatgtgctg cacacaccct gcgtggcatc gaagaccctg
     4981 tctgacatca ccggtgcgca ggtcttcctg aaatttgaaa acctgcagtt cactgcctcc
     5041 ttcaaggaac gcggtgcctg caacaaactc tcgcaactca cgccagagca gtctgcgcgc
     5101 ggagtgatcg cgatgagcgc aggcaaccat gcgcagggcg tggcctacca cgcgcaacgt
     5161 ctgggcttgc ggtccgtgat cgtcatgccc cggttcacac cgggtgtcaa agtggagcgc
     5221 acccggggct ttggcgcaga agtggtgctg cacggtgaca ccctggacga ggcccgcagc
     5281 cacgcactgg agttggcgac agcgcagcat ctcacctttg tgcacccgta cgacgacgaa
     5341 tccatcattg ccggacaggg cacggtcgcg ctggaaatgc tggaacaggt ggccgacctg
     5401 gactgcctgg ttattgcagt gggcggtggc ggactgattg caggtatggc cacggtggcc
     5461 aaggcgttgc ggcccggcat ggaagtggtg ggtgtgcaga ctgcacgctt tcccgccatg
     5521 gtcaacgcca tcaagggcac ccactacccg cagggcgcca gcacgattgc cgaaggcatt
     5581 gccgtgggca cacccgggcg cctgacccag gacatcatcc gcgagcgggt cgatgacctg
     5641 gtgctggtgg aggaaggtga catcgagcag gcgctggtca tgctgctgga agtcgagaag
     5701 accctggtgg aaggcgcggg cgccgccggc ctggcggccc ttctgaagta ccccgagcgg
     5761 ttccagggca agcgcgtggg tctggtcctg tgtggcggca acatcgaccc gctgttgctg
     5821 gctgccatca tcgagcgcgg catggttcgg gcaggacgcc tggcccgcat ccgggtgagc
     5881 gcacgcgaca ttccgggctc attggcgcgc atcacggcca cggtggcgga ggctggcgcc
     5941 aatattgacg aagtccacca ccagcgggcc ttcaccaccc tgtctgcgca aaacgtggaa
     6001 attgagctgg tcatccagac ccggggccat gcccatgtag cggatgtgct ggaagtattg
     6061 cgctccgcag ggtttgacgc gcagctcgtt taaggcaagt tggcgcctac tttcgcagaa
     6121 tttctccggg aaaaccacga tttttcaagg aatcgacgtg cataaagtta tgcctgtttt
     6181 gcataacttt tgaccgtaat cgcttggtaa cttgggtacc agttcttaaa attggcaccc
     6241 cggtcgcctt gggtcgtccg gttcgacagc tttcgaagat gtttatccaa agtgtgtggc
     6301 gcactttgga taaacgattt tcaaacttag gagctttccc atgaactcac cggtaatgca
     6361 gggcatcaac atcaatgcgc ccagctacgt caaaaacgcc aagctgattg cctgggtggc
     6421 cgaaatggcc gcactcacca agcccgcttc catttactgg tgcgacggct ccgacgagga
     6481 ataccagcgt ctgtgccagc agctggtcga cggcggcacc ttcaaaaaac tcaaccccgc
     6541 caagcgcccc aactctttcc tggcctgctc tgaccccagc gacgtggccc gcgtggaaga
     6601 ccgtacttac atctgcagcg aaaagaaaga aaacgctggc cccaccaaca actggatggc
     6661 cccggccgac atgcgcgccc tgctgcaaac cggcaaagac ggcgaaaagg ccctgttcga
     6721 cggctgtatg gcaggccgca ccatgtatgt ggtgccgttc tccatgggcc ccctgggttc
     6781 gcacattgcc cacgtcggca ttgagctgtc ggacagtgcc tatgtggccg ttaaccagaa
     6841 aatcatgacc cgcatgggca aggccgtgta cgacgtgatc ggcaccgacg gcgcgtttgt
     6901 gccctgcgtg cacactgtgg gcgcccccct ggccgctggc caggccgatg tgaaatggcc
     6961 ttgcaacaag accaagtaca tcgtgcacta ccccgaaacc cgcgaaatct ggtcctacgg
     7021 ctccggctac ggcggcaacg ccctgctggg caagaagtgc tacgccctgc gcatcgcctc
     7081 ggcgatggcc cgcgacgagg gctggatggc cgagcacatg c
//
LOCUS       DAOSJM010000078         7120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9583_length_7120_cov_4.747629, whole genome shotgun sequence.
ACCESSION   DAOSJM010000078 DAOSJM010000000
VERSION     DAOSJM010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9583_length_7120_cov_4.747629"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /gene="pheA"
                     /locus_tag="PKX82_04140"
     CDS             complement(<1..778)
                     /gene="pheA"
                     /locus_tag="PKX82_04140"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOF51471.1"
                     /translation="MARTLPELRVLIDAVDQELLTLLSRRASLAHEVGEIKKVEGSAV
                     FRPEREAQVIANLQQANQGGLKNESVAIIWREIMSACRALEAPQRVAYLGPKGTFSEE
                     AAQRFFGSSITHVPCANFDEVFHAATAGSAEFGVIPVENSTEGVVTRSLDLLLNSPLH
                     IVGEISLLVRHHLLRSTASLDGIEVVLAHPQALAQCQQWLTTHLPHAERRAVSSNAEG
                     ARLAASNPAWAGIASERAGSEFGLHIAAHAIQDEAFNRTRF"
     gene            complement(789..1916)
                     /gene="serC"
                     /locus_tag="PKX82_04145"
     CDS             complement(789..1916)
                     /gene="serC"
                     /locus_tag="PKX82_04145"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOF51472.1"
                     /translation="MQRPYNFSAGPAAMPAEVLAQAADEMLSWPDASGQRCGMGVMEM
                     SHRGKEFQSIYEAAEANLRELLAVPAQFKILFMQGGGLAENAIVPLNLSALRTPAGQP
                     GVTDFVVTGSWSEKSLKEAGKYSQANTAASGKDSGFTAIPPASSWHLSTDASYVHLCT
                     NETIHGVEFHTLPDLKALGSKAELVIDFSSHVASRPVDWSKVGLAFGGAQKNLGPAGL
                     TLVVVREDLLGHALPICPSAFNYKTVADNGSMYNTPPTYAIYMAGLVFQWLKRQGGIA
                     AMESRNIAKAELLYNTIDASGFYMNGVARGARSRMNVPFFLRNEALNDAFLAGAKAAG
                     LLQLKGHKSVGGMRASIYNAMPLEGVQALVQYMQEFERTHG"
     gene            complement(1998..4637)
                     /gene="gyrA"
                     /locus_tag="PKX82_04150"
     CDS             complement(1998..4637)
                     /gene="gyrA"
                     /locus_tag="PKX82_04150"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOF51473.1"
                     /translation="MTQFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLFAMHELNNDWNRAYKKSARIVGDVIGKYHPHGDQSVYDTIVRMAQDFSMRHML
                     VDGQGNFGSVDGDNAAAMRYTEIRLAKIAHELLADLDKETVDFGPNYDGSEKEPLVLP
                     TRIPNLLVNGSGGIAVGMATNIPPHNLNEVVDACLHMLRNPEATIDELMEIIPAPDFP
                     TAGIIYGINGVKDGYRTGRGRVVMRAKCHFEDIDKGQRQAIIVDELPYQVNKKTLQER
                     MAELVHEKKIEGISHIQDESDKSGMRLVIELKRGEVPEVVLNNLYKQTQLQDTFGINM
                     VALVNGQPKLCNLKDLISVFLNHRREVVTRRTVFNLRKARERGHVLEGLAVAMANIDE
                     FIRIIRESPTPPVAKVELMNRPWDSKLVREMLTRTRADGGVVNADDYRPDGLEKEFGM
                     GSDGLYRLSETQAQEILQMRLQRLTGLEQDKVIAEYKEVMAEIEDLLDILAKPERVST
                     IIGEELGHVKQEFGQTKLGARRSLVEHSSFDLSTEDLITPTDMVVTMSHSGYIKSQPL
                     HEYRAQKRGGRGKQATATKEDDWVDQLFVANTHDYILCFSNRGRLYWLKVWEVPQGSR
                     GSRGRPIVNMFPLQEGEKITVVLALTGEKRTFPADQYVFMATSMGTVKKTALDEFSNP
                     RKGGIIAVNLDEGDYLIGAALTDGKHDVMLFSDGGKAVRFDEDDVRPLGRNARGVRGM
                     SLDPSQSVIAMLVAEDEQQSVLTATENGYGKRTSITEYTRHGRGTKGMIAIQQSERNG
                     KVVAATLVHADDEIMLITDKGVLVRTRVSEIRELGRATQGVTLIGLDEGSKLSGLQRI
                     VENDANPADAEPGDDAGQEAEGT"
     gene            4878..5513
                     /locus_tag="PKX82_04155"
     CDS             4878..5513
                     /locus_tag="PKX82_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOF51474.1"
                     /translation="MKQLNKVALLIATAALATAAGAQEIHNWRSAAGDVWKNVDGQCW
                     RDASWTPATAAAGCGGALAAPAAPAAAAPAAAAAAAAAAPKAAAPAAAAAKVTYAADA
                     FFDFDKSVLKPEGKAKLDDLTGKVKGINLEVIIAVGHTDSTGADAYNQKLSVARAEAV
                     KAYLVSKGIEKNRVYTEGKGEKAPVADNKTKEGQAKNRRVEIEVVGTRASK"
     gene            5579..6289
                     /gene="ubiG"
                     /locus_tag="PKX82_04160"
     CDS             5579..6289
                     /gene="ubiG"
                     /locus_tag="PKX82_04160"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HOF51475.1"
                     /translation="MTTTLNADPAELAKFSELAHRWWDLDSEFRPLHQINPLRLDWID
                     KLSPISGLRALDVGCGGGILADSMARKGAHVVGIDLSTKALKVAQLHALEAQTPNVAY
                     REVSAEALAAEAPGAFDVVTCMEMLEHVPDPASVVQACATLVKPGGWVFFSTINRSAK
                     AFLYAIVGAEYLLKMLPQGTHEYAKMIRPSELAAYCRRAGLDLQQTRGLEYNPLTQRY
                     SLSADTGVNYMFAAQRPA"
     gene            6298..6972
                     /locus_tag="PKX82_04165"
     CDS             6298..6972
                     /locus_tag="PKX82_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOF51476.1"
                     /translation="MKVAFPNIEAVLFDLDGTLIDSAPDLGAAADQMRVARGLPSLPL
                     ETYRPMAGAGARGMLGIAFGISPESPGYDLLREEFFGNYAACMTERTYAFAGVSDLIA
                     EISARGLRWGVVTNKSARFTDPLTRAMPLFATAGAIVSGDTTPHAKPHPEPLFEAARR
                     LGLPPGRCLYVGDDERDVVAGLAAGMGTVAATYGYLGGKADTASWGAHAEINSPLDLL
                     QLLANA"
     gene            6985..>7120
                     /gene="ssrA"
                     /locus_tag="PKX82_04170"
     tmRNA           6985..>7120
                     /gene="ssrA"
                     /locus_tag="PKX82_04170"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1269 a         2096 c         2242 g         1513 t
ORIGIN      
        1 caaagcgggt gcggttgaaa gcctcatcct ggatcgcgtg cgccgcaatg tgcaggccaa
       61 actcggagcc cgcgcgctcg ctggcaatgc cggcccaggc cgggttgctc gcggccaggc
      121 gggcgccttc ggcattgctg gagacggcgc ggcgctcggc gtggggcagg tgggtggtca
      181 gccattgctg gcactgcgcc agtgcctgcg ggtgggccag cacgacttca atgccgtcca
      241 gcgatgcagt gctgcgcagc aggtggtggc gcaccagcag gctgatttcg cccacgatgt
      301 gcagcgggga gttgagcagc aaatcgagcg agcgggtcac cacgccctcg gtgctgttct
      361 ccacggggat cacgccaaac tctgccgagc cggcggtcgc tgcgtggaac acttcgtcaa
      421 agttggcgca aggcacgtgc gtgatgctgg agccaaaaaa ccgctgtgca gcctcttcgc
      481 tgaaggtgcc cttggggccc aggtaggcca cgcgttgcgg cgcttccagc gcacggcagg
      541 ccgacatgat ttcgcgccag atgatggcca cgctctcgtt tttgagccca ccctggttgg
      601 cttgctgcag gttggcgatg acctgggctt cgcgctcggg acggaacacc gccgagccct
      661 ccaccttctt gatttcaccc acctcatggg ccagggatgc gcggcgggag agcagggtca
      721 gcaattcctg gtcgacagcg tcgatcagga ctcggagttc ggggagggta cgtgccatgt
      781 tggttggttc agccgtgggt gcgctcaaat tcctgcatgt actgcaccag cgcctgtacg
      841 ccttccagcg gcatggcgtt gtagatgctg gcccgcatgc caccgaccga cttgtggccc
      901 ttgagctgaa gcagaccggc agctttggcg ccggccagaa aggcgtcgtt cagcgcttcg
      961 ttgcgcagga agaagggcac gttcatgcgc gagcgggcgc cccgggcaac gccgttcatg
     1021 taaaagccgg aggcatcaat cgtgttgtac aggagctcgg ctttggcgat gttgcgggat
     1081 tccatcgccg caataccacc ctgtcgcttg agccactgga acaccagacc cgccatgtaa
     1141 atggcatagg tgggaggcgt gttgtacatg gagccgttgt cggccacggt tttgtagttg
     1201 aaggcgctcg ggcagatcgg cagggcgtgg cccagcaggt cttcgcgcac gaccacgagg
     1261 gtgaggccag caggacccag gttcttttgc gcgccgccaa aggccaggcc cactttgctc
     1321 cagtccaccg ggcgcgaagc tacgtgagac gaaaagtcga tcaccagctc agccttggag
     1381 cccagggcct tgaggtccgg cagggtgtgg aattccacgc catggatggt ctcgttggtg
     1441 cacaggtgca catagctggc gtcggtgctc aggtgccagc tgctggcggg cggaatggcc
     1501 gtaaaaccac tgtctttgcc cgatgctgca gtgttggcct ggctgtactt gccggcttcc
     1561 ttgagtgatt tctcactcca gctgccggtc actacaaaat cagtcacacc aggctggcct
     1621 gcaggggtgc gcagggcgct gaggttcagc ggaacgatgg cgttttcagc cagaccgcca
     1681 ccctgcatga acaggatctt gaattgggcc ggcaccgcca gcaactcgcg caggttggct
     1741 tctgctgcct cgtagatgct ctggaactcc ttgccgcggt ggctcatctc catcacaccc
     1801 atgccgcaac gctggccgct ggcatcgggc cagctcaaca tctcgtcggc ggcttgcgcc
     1861 agcacttccg ctggcatggc ggccggtccg gccgagaagt tgtaagggcg ttgcatcgca
     1921 ggttatgggt taaatagggc tctagccccc gttaaatatg cgcaagcagc tattaacttt
     1981 atagcaattg tgctggctta ggtgccttct gcttcttggc ctgcatcgtc gcccggctcc
     2041 gcatcggccg ggttggcatc gttttccaca atgcgctgca gaccgctgag cttggaacct
     2101 tcgtccaggc caatcagggt tacgccctgg gtggctcggc cgagttcacg gatctcgctg
     2161 acccgggtgc gtaccagtac gcccttgtcg gtgatcagca tgatctcgtc gtcggcatgc
     2221 accagcgtgg ccgcgaccac tttgccgttg cgctcggact gctggatggc aatcatgccc
     2281 ttggtgccac ggccgtggcg ggtgtattcg gtgatgctgg tgcgtttgcc atagccgttt
     2341 tcggtggcgg tcagcacgct ttgctgctcg tcttctgcca ccagcatggc gatcacgctc
     2401 tggctggggt ccagtgacat gccgcgcacg ccgcgggcat tgcggcccag cggacgtaca
     2461 tcgtcttcgt caaagcgcac agccttgccg ccgtcactga acagcatcac gtcgtgcttg
     2521 ccgtcggtca gcgcagcgcc aatcagatag tcgccttcgt ccaggttcac ggcaatgatg
     2581 ccgcccttgc gtgggttgct gaattcgtcc agcgcggtct tcttgaccgt gcccatgctg
     2641 gtggccatga acacatactg gtcggccggg aaggtgcgct tttcaccggt cagtgccagg
     2701 accacggtga ttttttcgcc ttcctgcagc gggaacatat tgacgatggg gcggccgcgc
     2761 gaaccgcgcg agccctgcgg cacttcccag accttgagcc agtaaaggcg gccacggttg
     2821 gagaagcaca ggatgtagtc gtgggtgttg gccacgaaaa gctgatcaac ccagtcgtct
     2881 tctttggtgg ccgtggcctg cttgccgcgc ccgccgcgct tctgggcgcg gtattcgtgc
     2941 aggggctggc tcttgatgta gccgctgtgg ctcatggtca ccaccatgtc agtgggggtg
     3001 atcaggtctt cggtggacag gtcgaacgaa gagtgttcga ccaggctgcg gcgtgcgccc
     3061 agtttggtct ggccgaattc ctgtttgacg tgacccaact cttcaccaat gatggtggag
     3121 acgcgttcgg gcttggccag aatgtccagc aggtcttcga tctcggccat gacctctttg
     3181 tattccgcaa tgaccttgtc ctgctccagg ccagtcaggc gctgcaggcg catctgcaga
     3241 atttcctggg cctgggtttc tgacaggcgg tacaggccgt cgctgcccat gccgaattct
     3301 ttttccagac cgtcggggcg atagtcgtcc gcattgacca cgccgccatc ggcacgcgtg
     3361 cgggtgagca tctcgcgcac cagcttgctg tcccaggggc ggttcatcaa ctcgaccttg
     3421 gccacaggtg gtgtgggtga ttcgcggatg atgcgtatga actcgtcgat attggccatg
     3481 gcgacagcca gaccttccag cacatggccg cgctcgcgcg ctttgcgcag gttgaacacc
     3541 gtgcggcgtg tcaccacttc gcggcggtgg ttcaggaaga ccgaaatcag gtccttgagg
     3601 ttgcacagct tgggctggcc gttcaccagg gccaccatgt tgatgccgaa ggtgtcctgc
     3661 aactgggtct gcttgtacag gttgttcagc accacctcgg gtacttcgcc gcgcttgagt
     3721 tcgattacca ggcgcatgcc ggacttgtcc gactcgtcct ggatgtggct gatgccttcg
     3781 atcttcttct cgtgcaccag ctcggccatg cgctcctgca gcgtcttctt gttgacctgg
     3841 tagggcagct cgtccacgat gatggcctgg cgctggccct tgtcgatgtc ttcaaaatgg
     3901 cacttggcgc gcatcaccac acggccacgg ccggtacggt agccgtcctt cacaccgttg
     3961 atgccgtaaa tgatgccggc ggtggggaag tccggcgccg gaatgatttc catcagttca
     4021 tcgatggtcg cttccggatt gcgcagcatg tgcaggcagg cgtccaccac ctcgttgagg
     4081 ttgtggggcg ggatgttggt ggccataccg accgcaatac cgcccgaacc attgaccagc
     4141 agattgggga tgcgggtggg taacaccagc ggttcttttt ccgagccgtc gtagttgggg
     4201 ccgaagtcga ccgtttcctt gtccagatcg gccagcaact cgtgggcgat tttggccagc
     4261 cggatttcgg tgtaacgcat tgcggccgcg ttgtcgccat ccacggagcc gaagttgccc
     4321 tggccatcga ccagcatgtg gcgcatggag aaatcctgcg ccatgcgcac gatggtgtcg
     4381 tacaccgact ggtcaccgtg cgggtggtat ttaccaatga catcgccgac gatacgcgcg
     4441 gactttttgt aagcgcggtt ccagtcgttg ttgagctcgt gcatcgcaaa cagcacgcgg
     4501 cggtgtacgg gcttcaagcc atcccgcgca tcggggaggg cgcggcccac gatcacgctc
     4561 atggcgtaat cgaggtagct gcgacgcatt tcctcttcga ggctgatggg cagggtttct
     4621 ttggcgaact gggtcatggg aggatcgtgg ctatgtcagg gacaacccac gattctacga
     4681 cccggcgtat gtagcatggc tgcaacattt tgttgcactc gggcccgaga atggccctga
     4741 cctggtgcat atgatggaaa gcgctttttg ctatggcaca atagagcgac cgtcctaatt
     4801 ggtgcaatgc tgcttggggc gtcattcaaa tacatcgcag cgctgctgca gtttgctccg
     4861 ctcatagaag gaaagtcatg aaacaactga ataaagtagc actcctcatc gctaccgcag
     4921 cactggctac cgccgctggc gcacaggaaa tccacaactg gcgcagcgct gctggcgacg
     4981 tctggaagaa cgttgacggc caatgctggc gcgatgcaag ctggacaccc gccacggctg
     5041 ctgccggttg cggtggcgct ctggctgctc ctgctgctcc cgccgctgct gctcccgccg
     5101 ctgctgcagc cgccgctgcc gctgcaccta aagctgctgc tcccgctgcc gctgctgcca
     5161 aagtgactta cgctgctgac gctttctttg acttcgacaa gtccgttctg aagcccgaag
     5221 gcaaagccaa gctggacgac ctgaccggca aggtaaaggg tatcaacctg gaagtgatca
     5281 ttgctgtggg tcacactgac tccaccggcg ccgacgctta caaccagaag ctgtccgtgg
     5341 cacgtgctga agctgtcaag gcttacctgg tgtccaaggg catcgaaaag aaccgcgtgt
     5401 acaccgaagg caagggcgag aaggctcctg tggctgacaa caagaccaaa gaaggccaag
     5461 ccaagaaccg ccgcgttgaa atcgaagtgg tcggcacccg cgcttccaag taatctcttt
     5521 ttgagaaact tcaagaaacc cgcttcggcg ggttttttgt tgccctgata atctgtacat
     5581 gactaccact ctgaatgccg accccgctga gttggccaaa ttttccgagc ttgcccaccg
     5641 atggtgggat ctggacagcg aattccgccc cctgcaccag atcaacccgc tacggctgga
     5701 ttggattgac aagctgagcc cgatctcggg gcttcgggcg ctggatgttg gatgcggcgg
     5761 cggtattctg gcggattcca tggcgcgcaa gggtgcccat gtcgtgggca ttgatttgtc
     5821 caccaaggcg ctcaaagtgg cgcaattgca tgcgctggag gcgcagacac cgaatgttgc
     5881 ttaccgcgaa gtcagtgcag aagcgctggc agctgaagct ccgggagcgt ttgacgtggt
     5941 gacctgcatg gaaatgctgg agcacgtgcc agaccccgca tcggtagttc aggcgtgtgc
     6001 cacattggtc aaaccgggtg gatgggtgtt tttttcgacc atcaatcgca gcgccaaggc
     6061 gtttctctat gcaattgttg gcgcggagta ccttctgaaa atgctgcccc agggaacgca
     6121 cgaatatgca aagatgattc gccccagcga gctcgcggct tactgccggc gtgcagggct
     6181 ggatctgcag caaacccgcg gactggaata caaccccctg acgcaacggt attcactgag
     6241 tgcagacact ggtgtcaatt acatgtttgc ggcgcaaaga ccggcttgat tcgcgccatg
     6301 aaggtagctt ttcccaacat tgaagccgtt ctgtttgatc tggacggaac cctgattgac
     6361 agtgccccgg atcttggcgc ggcagccgac cagatgcggg tcgcacgggg attgccatcc
     6421 ctgccactgg aaacttaccg acccatggcg ggcgccggtg cccgggggat gctggggatt
     6481 gcctttggaa tttcgccaga gtctcccggt tatgacctgt tgcgtgagga gttctttggc
     6541 aattacgccg cctgcatgac cgagcgaacg tatgcctttg ccggggtttc ggacctgatt
     6601 gcggaaatct cggcacgtgg cctgcgctgg ggtgtggtga ccaacaagtc tgcgcgtttc
     6661 acggatccgc tcacgcgtgc catgcctttg tttgctacgg ccggagccat tgtcagcggg
     6721 gataccacgc cgcatgccaa gccgcacccc gagcccttgt tcgaggcggc ccggcggctg
     6781 gggctgccac ctgggcggtg cttgtacgtg ggtgacgatg agcgcgacgt cgttgcggga
     6841 ctggcggccg gcatgggcac cgtggcggcc acgtacggtt acctgggcgg caaggccgat
     6901 acggccagct ggggtgcaca tgccgagatt aattctccgc tggacctctt gcaattgctc
     6961 gcaaacgcct aaaatacgcc ttgaggggct gcactggttt cgacgtgggt tcggacgcgc
     7021 agcagggcat gtcgagctga atgcgctcgt aaaacagatt caaacaaact aactgcaaac
     7081 gacgaacgtt tcgcactcgc cgcttaattg cgcgtgagcc
//
LOCUS       DAOSJM010000079         7011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9771_length_7011_cov_3.892323, whole genome shotgun sequence.
ACCESSION   DAOSJM010000079 DAOSJM010000000
VERSION     DAOSJM010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9771_length_7011_cov_3.892323"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="PKX82_04175"
     CDS             <1..916
                     /locus_tag="PKX82_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOF51477.1"
                     /translation="RGQSVLLHKQCDNVWRIDFQLGWDADPTEEKKPEKVIPRIQAML
                     GPDVEFELEWVSVYQFACRRVDAFRHGRVLFTGDAAHQVSPFGARGANTGVQDCDNLS
                     WKLKLVLDGAAPLALLDTYHEERAFAADDNLLNSTRSTDFITPKSRCSLRLRNAVLEL
                     ARTEAFARPLVNSGRLSTPTPYAASSLNTPDADAFLGKMAPGTNCADAPIQADGKDAW
                     LLDHLGQGFVLLSFGPASPVSVGKVTARVLEVGKDIQDTHGVLAARYDAQPGTVYLIR
                     PDQHVAARWRQFDAAKATAALRHALALH"
     gene            933..1151
                     /locus_tag="PKX82_04180"
     CDS             933..1151
                     /locus_tag="PKX82_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HOF51478.1"
                     /translation="MPLNHQPNIASPDSFYEELIHAQRDMNDDQADMLLAKLVLILAN
                     HVGDRAVLSEALALARSNTLANTVASPA"
     gene            1148..2485
                     /gene="fahA"
                     /locus_tag="PKX82_04185"
     CDS             1148..2485
                     /gene="fahA"
                     /locus_tag="PKX82_04185"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HOF51479.1"
                     /translation="MTALNHTHAPDARSWVASANAVGTDFPIQNLPFAIFKRRGSAEA
                     MRGGIAIGDQVLDLQALASSGCLAGHALAHSAAEAASHSTLNALMALGPTAWSALRHA
                     VFDLLASGASAEVQEQVQACLIPQADVEYGLPAHIGDYTDFYTSIYHATNVGKLFRPD
                     NPLLPNYCWVPIGYHGRSSSVQVSGQPIHRPRGQVMAAGQTVPQLAPCARLDWELELG
                     VFMGPGNAPGRAIPLDAAPDHVFGMCLLNDWSARDIQAWEYQPLGPFLSKNFGTSISP
                     WVVTMEALAPYHCAMERPDGKQDDVDYLRSENNRNRGALDIQLRIGLTSDTMRAASQP
                     VHWVCQTSYRHAYWTLAQMVAHHTVNGCNLQPGDLLGTGTLSGPTLDQAGALLELTQG
                     GKVPLTLPDGQTRTFLQDGDEVTLQGWCEKPGAARIGFGECQARVLPATPTQT"
     gene            2605..3639
                     /locus_tag="PKX82_04190"
     CDS             2605..3639
                     /locus_tag="PKX82_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOF51480.1"
                     /translation="MKRLLTHTALAAGLLCAAWTAQAQTITLKVHHFLGPQSIQHTTM
                     LKTWCDNIAKDSNNRLNCQIYPAMQLGGTPPQLFDQAKDGVADVVWTVAGYSAGRFAR
                     SQVFELPFMMSNAGATSRAAWDFAQKYAQDEYKDVKLLAIHVHGPGVFFTKNKPITKI
                     EDLKGLKVRGPTATVTKMLGMMGATPVGMPVPAVPEALSKGVIDGAVIPFEVAPGLKV
                     HELTKFSTETEKSSPALYTTVFVVPMNKAKYESLPADLKAVIDKNSGREFSAFLASTQ
                     EGNDVPSRKVFADTAGYTMTTLAHSELERWKKATATLDDAWISDMNQRGFQGQAMYDD
                     AVKMIKGYTK"
     gene            3747..4268
                     /locus_tag="PKX82_04195"
     CDS             3747..4268
                     /locus_tag="PKX82_04195"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOF51481.1"
                     /translation="MTDILVTEDTTERHGIALMLDRTCKLLAILGGVTLVAMALMSLW
                     SIVGRSFFESPLLGDYELVQMMSGMAVAMTLPYAQWIGGHVIVDFFTAKAPVKANAFM
                     DLVAALLMALFAAVITWRMAVGLVDLKSTFDASMMLNLPTWWSYVPMTISFALLTATA
                     LFTATTNLRKLLK"
     gene            4265..5581
                     /locus_tag="PKX82_04200"
     CDS             4265..5581
                     /locus_tag="PKX82_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOF51482.1"
                     /translation="MSPIEWGALMFGVLLLLLAVRVHIGIAMLMTGSIGYVFVAGFDP
                     LMNYFKSAAYARFSVYDLSVVPLFLLMGQFATHGGLSKALFQAGNAFIGHWRGGMAMA
                     GVTACAGFGAICGSSLATAATMGQVALPELKSHGYSGRFSTAVLAAGGTLGILIPPSV
                     PLVIYAILTEQNIAKLFLAAFIPGILAAIGYMVVIAFVARVSPSDAPAGDRYSWPQRL
                     QALVETWPVMLIFAVVIGGIYGGFFTPTEAASIGTVATAFVAWRSGGLRGKGFLQCMY
                     GTAAATGMIFLILLGADVLNVFLVLTQVNVALANWVIGLHVSPILVMFVIIFIYLVLG
                     CIMDSLSMILLTIPIFFPIVMGLDLWGLDPESKAIWFGIIVLMVVEIGLITPPVGMNV
                     FIINSMAKDVPMKETFKYVMPFLASDLLRIVLIVFVPSIVLFVLKI"
     gene            5596..6141
                     /locus_tag="PKX82_04205"
     CDS             5596..6141
                     /locus_tag="PKX82_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOF51483.1"
                     /translation="MSKASTAASQDTPIEDFSQCHAGIFRKLDQLGELPALLAPAVRA
                     REIAEKSLEFFREAIFEHHLDEERELFPAVVAHAEKGAEFDKVQFMVARLTIEHRDLE
                     AVWKRLESGLKAVAKGRDSDINVADIELLVTRYRGHAQYEETEFLPLAQTILSRNSNH
                     MAALGMSLHMRHAPLQVVPFV"
     gene            6249..6401
                     /locus_tag="PKX82_04210"
     CDS             6249..6401
                     /locus_tag="PKX82_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF51484.1"
                     /translation="MRAATSGTNPCTNPWLLYQETATRVDLEAIEALCKYFNVQVGEL
                     FELTDA"
BASE COUNT         1407 a         2171 c         1986 g         1447 t
ORIGIN      
        1 ccgcggccag agcgtgctgc tgcacaagca gtgcgacaac gtctggcgca tcgactttca
       61 gttgggctgg gacgccgacc ccaccgaaga gaaaaaaccc gagaaagtca ttccgcgcat
      121 ccaggccatg ctgggcccgg atgtggagtt tgagctggag tgggtatcgg tgtaccagtt
      181 tgcctgccgc cgcgtggatg ccttccgcca tggccgggtg ctgtttaccg gcgacgcggc
      241 ccaccaggtg tccccctttg gtgcccgcgg tgccaatacc ggcgtgcagg actgcgacaa
      301 cctctcctgg aagctcaagc tggtactgga cggtgcagcc cccctggcat tgctcgacac
      361 ctaccatgaa gagcgcgcgt ttgcggcgga cgacaacttg ctgaactcca cccgttccac
      421 cgacttcatc acgcccaaaa gccgctgctc gttgcgcctg cgcaatgcag ttctcgaact
      481 ggcgcgcact gaagccttcg cacgcccctt ggtcaacagc ggtcggctct ccacgcccac
      541 tccgtatgcg gcctcatcgc tcaatacgcc cgatgccgat gcattcctgg gcaagatggc
      601 accgggcacc aattgcgcag acgctcccat ccaggccgat ggcaaggatg cctggttgct
      661 ggaccacctg ggtcagggat ttgtgctgtt gagttttggc cctgcttcgc cggtgtccgt
      721 gggcaaagtg actgcccgcg tgctggaagt cggcaaagac attcaggaca cgcatggcgt
      781 gctggcagcg cgctatgacg cgcagcccgg caccgtgtac ctcatccgcc ctgaccagca
      841 tgtggctgcc cgctggcgcc agtttgacgc tgccaaagcc accgcagccc tgcgccacgc
      901 actggcattg cactgaccca ggaccaccca ccatgccact gaaccaccaa cctaatattg
      961 cgtctcccga cagcttttac gaagagctga tccatgcgca acgcgacatg aacgacgacc
     1021 aggcggacat gctgctggcc aagctggtgc tgatactggc caaccatgtc ggcgaccggg
     1081 ccgtgctctc ggaagcactg gccctggcac gcagcaatac gctggcaaac accgtagcca
     1141 gcccagcatg accgcactga accacaccca tgcgcctgat gcacgcagct gggtagccag
     1201 cgccaatgcc gttggcacgg acttccccat ccagaacctg ccctttgcca tcttcaaacg
     1261 tcgcggcagc gccgaggcga tgcgtggtgg tatcgcgatt ggcgatcagg tgctggacct
     1321 gcaggcactg gcatccagtg gctgcctggc tgggcatgca ctggcacaca gcgcagcaga
     1381 agcagcatca cattccacct tgaacgcctt gatggcactg ggacccacgg cctggagcgc
     1441 cctgcgccac gccgtgtttg atctgctggc aagtggcgca agcgcagagg tgcaggaaca
     1501 ggtgcaagcg tgcctgattc cgcaggcaga cgtggaatac gggttgcctg cacacattgg
     1561 tgactacacc gacttctata cctccatcta ccacgccacc aatgtaggca agctgttccg
     1621 ccccgacaac ccgctgctac ccaactactg ctgggtgccc atcggctacc acggtcgctc
     1681 ctccagcgtg caggtatccg gtcaacccat ccaccgcccc cgcgggcaag tcatggccgc
     1741 tggccagacc gttccgcagc ttgcgccctg tgcacgactg gactgggagc tggagctggg
     1801 ggtgttcatg ggccccggca atgccccagg gcgcgcgata ccactggatg ctgcacccga
     1861 ccacgtattc ggtatgtgcc tgctcaacga ctggtctgca cgcgacatcc aggcctggga
     1921 ataccagccc ctgggtcctt tcctgtccaa gaattttggc accagcattt ccccctgggt
     1981 ggtgaccatg gaggcgctgg ctccctacca ctgcgcgatg gagcggcccg acggcaagca
     2041 ggacgacgtg gactacctgc gcagcgaaaa caaccgcaac cgtggtgccc tggacatcca
     2101 gctgcgcatt ggactcacca gcgacaccat gcgcgcggcc agccaacccg tgcattgggt
     2161 atgccagacc agctaccggc atgcctactg gacactggcc cagatggtgg cacaccacac
     2221 cgtcaacgga tgcaacctgc agcccggcga tctgctgggt actggaaccc tgtcgggccc
     2281 cacactggac caggcgggtg ccttgctgga actcacccag ggcggcaaag tgccactaac
     2341 attgcccgat ggacagacac gcactttttt gcaggatggc gatgaagtca ccctgcaagg
     2401 ctggtgtgaa aagccaggtg cagcccgcat cggtttcggg gaatgccagg cacgggtgtt
     2461 gcccgcgaca cccacccaaa catgaataat aatgcatatg acattccaaa atacccgaac
     2521 acagggtaaa ccccccgagt tgcggggcac gcaatcagta acataatgca tgtcgcatta
     2581 ccatcccaaa gaaggagaca aaccatgaaa cgcttactca cccacactgc cctggccgca
     2641 ggcttgctgt gcgcagcctg gaccgcacaa gcccagacca tcaccctgaa agtgcaccac
     2701 tttctgggac cacagtccat ccagcacacc accatgctga aaacctggtg cgacaacatc
     2761 gccaaggatt ccaacaaccg cctgaactgc cagatctacc ctgcaatgca attgggtggc
     2821 acaccgccac agctgtttga ccaggccaag gacggcgtgg ctgacgtggt gtggaccgtg
     2881 gcaggctact cggctggccg gtttgcccgc tcgcaggtgt tcgagttgcc cttcatgatg
     2941 agcaatgcgg gtgccaccag ccgtgcagcc tgggactttg cacaaaagta cgcgcaagac
     3001 gaatacaaag acgtcaagtt gctcgccatc cacgttcacg gcccgggtgt ctttttcacc
     3061 aagaacaagc ccatcacaaa gattgaagac ctcaaaggtt tgaaggtgcg tgggccaacc
     3121 gccacagtca caaaaatgct cggcatgatg ggcgccaccc cggtcggcat gcccgtgcct
     3181 gcggtacccg aagcgctgtc caagggcgtg attgatggtg cagtgatccc gtttgaagtg
     3241 gctcccggct tgaaagtgca cgaactcacc aagttctcga cagaaaccga gaagagctcg
     3301 cctgcgctgt acaccacggt gttcgtggtc cccatgaaca aggccaagta cgagtccctg
     3361 cctgcagacc tgaaagccgt gatcgacaag aactcgggcc gcgagttctc cgcgttcctc
     3421 gcgtccaccc aagagggtaa cgatgttccc agccgcaagg tcttcgccga caccgcgggt
     3481 tacaccatga ccacccttgc ccactccgaa ctggagcgct ggaagaaagc cacagccaca
     3541 ctggatgatg catggatttc cgacatgaac cagcgtggct tccagggcca ggccatgtac
     3601 gatgacgccg tcaagatgat caagggttac accaagtaac tgcacgatcg ctgaagcagc
     3661 ccaagggcgg agaattctcc gcccttgtca tctgtgccag ccctaaccgc ccctacgtcg
     3721 agaccttctt tttctggagc caacccttga ccgatattct tgtcaccgaa gacaccaccg
     3781 agcgccacgg cattgcactg atgctggacc gcacgtgcaa attgctcgcc atactgggcg
     3841 gcgtcaccct ggtggcgatg gccctcatgt ccctctggag cattgtgggc cgctcatttt
     3901 ttgaatctcc tctgttggga gactatgaac tcgtacagat gatgagtggc atggcagtgg
     3961 ccatgacatt gccgtacgcc caatggatcg gcggccacgt catcgtggat tttttcaccg
     4021 ccaaggcacc cgtaaaagcc aatgccttca tggacctggt tgccgccttg ctgatggccc
     4081 tgtttgcagc agtcatcacc tggcgcatgg ctgtgggcct ggtagacctg aagtccactt
     4141 ttgatgcctc catgatgctg aatctgccga cctggtggag ttatgtcccc atgactattt
     4201 cctttgcgct gctgacggct accgcccttt tcaccgccac caccaacctg agaaagttgc
     4261 tgaaatgagc ccaatcgaat ggggtgccct gatgttcggg gtgctgttgc tgctgctggc
     4321 ggtgcgggtc cacattggta ttgccatgtt gatgaccggc tcgattggct atgtgttcgt
     4381 tgcggggttc gacccgctca tgaactattt caagtccgcc gcttacgcgc gcttctcggt
     4441 ctacgacctg tcggtggtgc cactgtttct gctgatggga cagttcgcca cacacggtgg
     4501 attgtccaaa gcgctgttcc aggctggcaa tgcgtttatc ggccactggc gcgggggcat
     4561 ggcgatggcc ggcgtcacgg cctgtgcggg cttcggagcc atttgcggat cgtccctggc
     4621 cacagcggcc accatgggcc aggtggcatt gcccgaactc aagtcgcacg gctactctgg
     4681 ccggttctcg accgcggtgc tggccgccgg tggcaccctg ggcattctga ttcctccttc
     4741 tgtgcccctg gtgatctacg cgatcctgac cgagcagaac attgccaagc tgtttctggc
     4801 ggccttcatt ccgggcattc tggcggccat tggctacatg gtggtgattg ccttcgtggc
     4861 ccgtgtcagt ccttccgacg cacccgcggg tgaccgctac tcctggccac aacggctgca
     4921 ggcactggtg gaaacctggc cggtcatgct gatctttgcc gtggtgattg gtggtattta
     4981 cggcggattt ttcaccccca ccgaagcggc atcgatcggc acggttgcta cagcttttgt
     5041 agcgtggcgt tcaggcggac tgcggggaaa aggctttttg cagtgcatgt atggcacggc
     5101 agcggccacc ggcatgatct tcttgatctt gctgggtgcc gatgtgctga acgtgtttct
     5161 ggtactgacc caggtgaatg tggcgctggc caactgggtg atcggtctgc atgtgtcacc
     5221 cattctggtg atgttcgtga tcatcttcat ctacctggtg ctgggctgca tcatggacag
     5281 cctgtccatg attctgctga ccatcccgat cttcttccct atcgtgatgg ggctggacct
     5341 gtgggggctc gatccggagt ccaaggccat ctggttcggc atcatcgtgc tgatggtggt
     5401 cgagatcggc ctgattaccc ccccggtggg catgaacgtg ttcatcatca acagcatggc
     5461 caaagacgtg cccatgaagg aaaccttcaa gtacgtcatg cccttcctgg cgtctgacct
     5521 gctgcgcatc gttctgatcg tgttcgtgcc cagcattgtg ttgttcgtgc tgaaaattta
     5581 atgaccggag ccgatatgtc caaagcatcc accgccgcaa gccaagacac cccgattgaa
     5641 gacttctccc aatgccatgc aggcatcttc aggaagctgg accagttggg cgaactgcct
     5701 gccctgctgg caccggcagt gcgggcacgt gaaattgccg agaagtccct ggagttcttt
     5761 cgcgaagcga tttttgaaca ccacctggac gaagagcgcg agttgtttcc cgccgtcgtg
     5821 gcccatgcag aaaagggcgc ggagtttgac aaagtgcagt tcatggtggc gcgcctgacc
     5881 attgagcacc gcgatctgga agccgtctgg aaacggctgg agtctggcct caaagccgtc
     5941 gccaagggcc gtgacagcga tatcaatgtt gctgatattg aactgctggt cacccgctac
     6001 cgcggccacg cgcagtacga agagaccgag tttctgccac tggcccagac cattttgagc
     6061 cgcaactcca accacatggc agcactcggt atgtccctgc acatgcgcca tgcgccattg
     6121 caggtcgtgc cctttgtctg atcgtatgtc taagcgctaa tcccgctcga accgatgggc
     6181 cagcagggtg gatgccgcag gcaccacacc gctggccagt tgcaggtgct ggtccagata
     6241 accttcggat gcgggcagca acctctggta caaatccctg cacaaatccc tggctgttat
     6301 atcaagagac agcaactcgt gtcgacctgg aagcaatcga agcactctgc aagtacttca
     6361 acgttcaggt tggcgaactg tttgaactta ccgacgctta agcttgaccc gcttcgggcc
     6421 atctgcagtc actggccaat gtcagcttac gagtcgttcg attctgtacc ttccgtgccg
     6481 acgaaaaact gtcaccggcc cctcgaggca cacagcggta gtcgtaattt tcgatctggc
     6541 tacttcggac cggtcactgg caaccatgga tcgatgggca gctatgtagg aagccctcgg
     6601 tcaagcctag cacccatcat gttgctacgc cgtcattcat gaatcgctgc tatttgattc
     6661 gcgatgggtt attgcttgtc ctttccctgc cgcgtggtgg ggcggggcca cggtggtagc
     6721 gttgctgtgc agtgcgatcc gaatatcttg ttatagacga atacctgacg aaaaaagtga
     6781 tagcagccag tttgggaaat ggcccctacg taacctacac tgggaaatca tttttataag
     6841 aattgaggcg ctatggtgac cacgacttgg cggcaagggc tgacgctgct tatgggctgg
     6901 gcggtcgctc tgctaggcgc cacggtcatg gtgggctggc acctgcacat aaagccgtta
     6961 ctgcaaattc atcccagttt tgtacccatg cagtacaaca cggccctggg t
//
LOCUS       DAOSJM010000080         6991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9803_length_6991_cov_3.751730, whole genome shotgun sequence.
ACCESSION   DAOSJM010000080 DAOSJM010000000
VERSION     DAOSJM010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9803_length_6991_cov_3.751730"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3553)
                     /locus_tag="PKX82_04215"
     CDS             complement(<1..3553)
                     /locus_tag="PKX82_04215"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOF51485.1"
                     /translation="MNALISLFLAWKRRIAWTVGSLLVVWAVAWLAVPGLVKSQVETR
                     GSEALGRKLTVGAIDFKPWSLELTISDLAIASADGATSQFKVARVYVDAELQSLLRLA
                     PVVDAITVDAPQLQVTHLSAGHYDLDDVVARLNQPSKEPASAPLRFALHNLTLNGGEV
                     DFTDRLNGTERKHVVRKLHVALPFLSSLASQREVNVTPRLAFELNGSAFDTAAEGTPF
                     AQTRKGEATVRISQFDVTPYLPYLPKGLPLQLKAAVLDTDLRVAFEQQPRLAVSVKGS
                     ATVSSLDLAEAGGQDLLGVESIHAVLADVRPLEQVVRLESLEIVAPELQVRRNRAGHI
                     NLDLATSVKSKNAPEIIAASASNTGATGQNDAKPATATPAQTTQPAWQVELARFALHK
                     GAVHWTDDTLAPSAKRDVKDIELQVQAIQWPSTDKPATLEGSLAIPSGNKTAQFAFKG
                     TGTDGAGSVEATLKDLPLGVTAPYVAQFLVPQVAGVLEAEAQALWKDGQVQLKLPRLA
                     LRDFALVADKALAAESAAPLPRFKWLEITDAQADLTARTASVGKVALRAPSATVDRDA
                     QGRWMFQRWLKTAPAADTPAPASAANTAATTPPWKVAVAELALDDGAVALDDRLPARP
                     VMATISALKVLAKTLTLDGKKPAPLTVLARIKAGQTEPGMLKYSGTVMWDPVTVQGTL
                     DALDIPAHAFAPYVADRLNIELLRADASVKGTVRYAATAGGPQVQLRMDAALEDFRSN
                     SVQTTGGQGLPVGEELLSWKSLNVPGIEFDMVPGTALRVQVREAALTDFYARLLISPQ
                     GRLNLQDLVKPAPADVGKVAAAPATAASAPVASAPATPAGPAPIITVGPVSLVHGKVL
                     FSDRFIQPNYSADLSELTGRLSKFSSQSVDGVVQLADLELRGRAEGSAALEITGKINP
                     LARPLALDVRGKVRDLELPPLSPYAVKYAGYGIERGKLSVDVSYLIKPDGQLTATNSL
                     VLNQLTFGDKVDGAPNSLPVKLAVSLLADRNGVIDLNLPISGSLNDPQFSIGPVIWKV
                     ITNLIAKALTSPFSLLASALGGSDELSNVSFAPGSSVLTADARQGLDKVAQALVDRPG
                     LKVTVLGTASLEAEREALKRERLRGLLLAEKRRQAAVAGQDTVAVTAVTDAEYPALLK
                     EVYRRADITKPRNLVGFAKTLPAPEMESLLLASIGVNE"
     gene            complement(3736..4323)
                     /locus_tag="PKX82_04220"
     CDS             complement(3736..4323)
                     /locus_tag="PKX82_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51486.1"
                     /translation="MAVFAQSVGALLLLGMGVVVLLLMARMRLQKFFAGVLGVAVVCG
                     AVYLSGVVPVTHLGKETAVGRKVVEAFKASGRGSFTWRISQDQKAIALIKPVAVIGHG
                     QWDWWRSLGTRPWGLVLLLVGQFGLMGLACAFGALLAPAMVTLWRAPIARCWRPAGAS
                     IVLAIMTVLMALDAMLNSFIFFPALMAAGALVSDK"
     gene            complement(4339..4872)
                     /locus_tag="PKX82_04225"
     CDS             complement(4339..4872)
                     /locus_tag="PKX82_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51487.1"
                     /translation="MLIAKAWIAPASAMLGVIWKDRQRLSGLRWSALDMPILLWCVWP
                     LLQWTVRPEVLPDAVLSSVYLSGSWGLTWLLGRLYFGNQEAQGALLHGLAWAGLVCLP
                     IALWEGISGPDLYAWLYHPHPFVSDGAERYLGWRPLVFFENGNQYGLWVSLCALASIW
                     VWRCRVVGKQSTWWAGG"
     gene            5284..5646
                     /locus_tag="PKX82_04230"
     CDS             5284..5646
                     /locus_tag="PKX82_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51488.1"
                     /translation="MRHASHDLVTAALQSAGLYQGRAIVQKWLLVAVLGAALVLALTR
                     WNTKANARRRTHAGLALACTAATWTLFLVETVSLHAVDRVLYRPIGPVLLIAYLWVTN
                     AMVVVWLARRSQLHRPGF"
     gene            5725..>6991
                     /locus_tag="PKX82_04235"
     CDS             5725..>6991
                     /locus_tag="PKX82_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HOF51489.1"
                     /translation="MAAWAILWVLLPMGASAAPSPLVLSDTTPQTEVWSYVTVLPEGG
                     KKLGIRDAMGALGQFTKPDTAYGTLGLQKDAVWLHIPVSVSGDGDGLWVLDIDYPVLN
                     RIDVYAVHGQNIVVQTRVGSLQPPEDPRARARSHAVSLDLQPGARYDLYMRVESKGSL
                     ILPMTLSKPYAFLVTALKEQMLQGLLTGLALCLLVYSLAQWITLGEHLYVKYAILITG
                     SMLFSLVQFGIGSQYLWPGNAWAETHMAGLSALVAATGSFLFIEQALKGPDTLRWFSW
                     LMKAGAVFTVFFAVCFSLDLIDVYIVTAVISSLGLAPALLGLPGAISRARRGDSVGFY
                     FLLAWAVYFVTTAVLIEVIKGRVGVNFWTLHSFQLGATFDMLIFMRVLGLRTKALQAA
                     AQHAKREHDHLHSMAHTDPLTGLSNRRGLN"
BASE COUNT         1378 a         2299 c         2033 g         1281 t
ORIGIN      
        1 cctcattgac tccgatgctg gccagcagca aggactccat ctcgggtgcc ggcaaggttt
       61 tggcaaaacc caccaagttg cgtggcttgg taatgtcagc gcgccggtac acctccttga
      121 gcagtgcggg gtactcggcg tcggtcacgg cggtcaccgc caccgtgtcc tgcccggcca
      181 cggcggcctg gcggcgcttc tcggccagca acaggccgcg cagtcgctcc cgcttgaggg
      241 cctcccgttc agcctccaga cttgcggttc ccagcaccgt gaccttcagg ccgggccggt
      301 ccaccaaggc ttgggccact ttgtccaggc cctgtctggc atccgctgtc agcacactgg
      361 agccaggagc aaagctgaca ttgctcagtt cgtcgctgcc gcccaaggca ctggccaaca
      421 ggctgaacgg tgacgtcagg gccttggcaa tcaggttggt gatgaccttc cagatcaccg
      481 gcccgatact gaactgcggg tcgttcagcg aaccgctgat gggcaggttc aggtcgatca
      541 cgccattgcg gtcggccagc agcgacaccg ccagcttgac cggcaggctg ttgggcgcac
      601 catccacctt gtcgccaaaa gtcagctggt tcagaaccag gctgttggtg gcggtcagtt
      661 gcccatcggg tttgatcagg tagctgacgt ccacactgag cttgccgcgc tcgatgccgt
      721 agcccgcgta cttgaccgca tagggcgaca ggggcggcaa ctccaggtcg cgcaccttgc
      781 cgcgcacgtc cagcgcgagt ggcctggcca gagggttgat cttgccggtg atctccagcg
      841 ccgcactgcc ttcggcgcgc ccgcgcaact ccaggtcggc cagttgcacc acaccgtcca
      901 cactttgcga ggagaacttg ctgagccggc cggtgagttc gctcaggtcg gcagaatagt
      961 tgggctggat gaaacggtcc gaaaaaagta ccttgccgtg caccagactg accggcccca
     1021 cggtgatgat gggcgcggga ccagccgggg tcgcaggtgc ggacgccaca ggtgctgaag
     1081 cagctgtggc aggggcagca gccactttgc cgacatctgc tggtgcgggt ttgaccaggt
     1141 cctgcaggtt gagcctgcct tgcgggctga tcagcagacg ggcatagaaa tcggtcagcg
     1201 cagcctcgcg cacctgcacg cgcagggcgg tgcccggcac catgtcgaac tcaatgcccg
     1261 gcacgttgag cgacttccag ctcagcaact cttcccccac cggcagcccc tggccacccg
     1321 tggtctgcac actgttcgag cgaaaatcct ccagcgcggc atccatgcgc aattgcactt
     1381 gcggcccgcc cgcagtcgcg gcatagcgca ccgtaccctt gacactggca tccgcgcgca
     1441 gcagctcgat attgagccgg tctgccacat agggggcaaa ggcatgggcg gggatatcca
     1501 gtgcgtccag cgtaccctgc accgtgaccg ggtcccacat cacagtgccg ctgtacttga
     1561 gcatccccgg ctcagtctgg cctgccttga tgcgggccaa gacggtcaga ggggccggct
     1621 tcttgccgtc gagcgtcagc gtcttggcca gcaccttcag tgcggagatc gttgccatca
     1681 ctggccgcgc aggcaggcgg tcgtccagtg ctaccgcgcc gtcgtccagg gccaactcgg
     1741 ccaccgccac tttccatggc ggcgtagtcg ccgcggtatt cgcggcagag gcaggagctg
     1801 gtgtgtcagc tgcaggagcc gtcttcaacc agcgctggaa catccagcgc ccctgcgcat
     1861 cccgatccac cgtggccgaa ggtgcacgca gcgccacctt gcccacgctg gcggtgcggg
     1921 cggtcaggtc tgcctgtgca tcggtgattt ccagccattt gaatctgggc aatggcgccg
     1981 ccgattcggc agccagtgcc ttgtcggcca ccagcgcaaa gtcgcgcaag gccagccgtg
     2041 gcagtttgag ctgcacctgc ccgtccttcc acagcgcctg ggcctcggcc tccagcacgc
     2101 cagccacctg cggtaccagg aattgcgcca catagggcgc agtcacgccc agcggcaggt
     2161 ccttcaatgt ggcctcgacg ctgccagcgc catcggtgcc tgtgcccttg aaagcgaact
     2221 gggcggtttt gttgcccgac ggtattgcca gactgccttc cagcgtggcc ggcttgtcgg
     2281 tagagggcca ctggatcgcc tggacctgca gttcgatgtc cttcacatcc cgctttgcac
     2341 tgggtgccag ggtatcgtcg gtccagtgca ccgcgccctt gtgcaaggca aagcgcgcca
     2401 gttccacctg ccatgccggc tgggtggtct gcgctggcgt tgcagtcgca ggtttagcat
     2461 cattttggcc tgtagccccc gtattgcttg cgctagcagc tatgatttca ggagcatttt
     2521 tcgatttgac ggaggttgcc aaatccagat tgatatggcc cgcacggtta cgccgcacct
     2581 gcagctccgg cgccacgatc tccagcgact ccagccggac cacctgctcc agcggacgca
     2641 catccgccag caccgcatgg atggactcca ctcccagcag gtcctgacca ccagcctccg
     2701 ccaggtccag tgaggacaca gtggccgaac ctttgacgga cacggccagg cggggctgct
     2761 gctcgaaggc cacgcgcaaa tcggtgtcca gcaccgccgc cttgagctgc agcggcaagc
     2821 ccttgggcag gtaaggcagg tacggcgtca catcaaattg ggagatgcgt accgtggcct
     2881 cgcctttgcg ggtctgggca aacggggtgc cctcggccgc ggtatcaaag gcgctgccat
     2941 tgagttcaaa tgccaggcgg ggcgtgacat tcacttcgcg ctgggacgcc aggctgctca
     3001 ggaagggcag cgcgacatgc agcttgcgca ccacgtgctt gcgctccgtc ccattcagac
     3061 ggtccgtgaa gtccacctcc cctccattga gggtcaggtt gtgcagtgca aaccgcaggg
     3121 gggcactggc cggttccttg gaaggctggt tcagccgggc cacgacgtcg tccaggtcgt
     3181 agtgcccggc actcaggtgg gtaacctgca gttgcggcgc atcgaccgtg atggcatcga
     3241 ccaccggcgc caggcgcaac agcgattgca actcggcatc cacatacacc cgcgccacct
     3301 tgaactggct ggtcgcgcca tcggccgatg caatggccag atcgctgatc gtcaactcca
     3361 gcgaccaggg cttgaaatcg atcgcgccca cggtgagctt tcggcccagg gcttcgctcc
     3421 ctctggtctc cacctggctc ttcaccagtc cgggcacagc cagccaggcc actgcccaca
     3481 ccaccaacag gctgccgaca gtccaggcga tgcgacgctt ccacgcaaga aagagagaga
     3541 tcagagcgtt catacaaaaa aacaaaaaaa gggcccgcag gcccggggtc agtccgaagt
     3601 gaagtgatca tcaaccggca ggatgcgcac aacgggccct tgaagagaat ggcatgaatc
     3661 caacggtccc gctagtgtca catattccgg attccagcca tccgccaagc cacttcctgt
     3721 ggcagatcgg cgtctttatt tgtcagaaac cagcgcaccc gccgccatca gcgcagggaa
     3781 gaagatgaaa gagttcagca tcgcatccag cgccatcaaa acggtcatga ttgccagaac
     3841 gatggaggca cctgcagggc gccagcatct ggcgattggc gcccgccaga gcgtcaccat
     3901 ggcaggggcc aatagcgcac cgaatgcaca cgccaaaccc atcaatccga actgaccgac
     3961 cagcaaaagc accagccccc aagggcgcgt acccagcgaa cgccaccagt cccactgacc
     4021 atgcccgatc accgcaaccg gctttatcag ggcaattgcc ttttggtcct gagagatgcg
     4081 ccaggtgaac gacccgcgcc ctgacgcctt gaaagcctcc accaccttac gccccacagc
     4141 agtctctttt cccaagtgag tgacaggcac cacgccactg aggtacaccg ccccgcagac
     4201 aaccgcaacg cccagtacac cggcaaagaa tttctggaga cgcatgcgcg ccatcaagag
     4261 caatacgacc acacccatcc cgagcaataa cagagccccc accgactgcg cgaaaaccgc
     4321 catggcgacc actgcgacct acccacccgc ccaccaggtg ctttgctttc ccaccactct
     4381 gcaccgccag acccagatgg aagccaacgc gcacaggctc acccaaagac catattgatt
     4441 gccattctcg aaaaaaacca ggggacgcca tcccaggtac cgctccgcgc catcgcttac
     4501 gaaagggtgg gggtggtaca gccacgcgta caggtctggg ccagagatgc cctcccatag
     4561 cgcaatgggg agacagacca accctgccca cgccagcccg tgcaacagcg caccctgcgc
     4621 ctcctgattt ccgaaataca ggcgcccaag cagccaggtc agcccccaac ttccggataa
     4681 gtaaacactg gacaacacgg catccggcag tacttccggc ctgaccgtcc actgcaatag
     4741 cggccacaca caccagagca atatgggcat atccagagca ctccaccgaa gaccagaaag
     4801 acgctgccga tccttccaga tcacacccag cattgcgctg gcaggtgcga tccacgcctt
     4861 ggcaatcagc atgtccgatg gcaaggcagc cccaaccaac caatagggaa aatccaatgc
     4921 actggcacct gacgggtaca cccccactgg cagtaccaac cagcccccca gaaacaccaa
     4981 cagcaccgcg agctgcggac ggaccatcgc aaagataatg accgccaaca acggccaagc
     5041 aagcaaggca accgttacat tcactgtacg caccccaagt tcaatacgga agatttcctg
     5101 acgatggtaa tggactcacc cccgaacccg acccagatcg cggggatgct tgcatttgca
     5161 gtgagcgcgc tcactgccgc ctacgcagcc agaaatgcgc gtttgcattt gcctgcaacc
     5221 gctaggacct ggtggtggac cagtgctttg cagctgctgt tgtgcatgga ggtatggctt
     5281 ggcatgcgtc acgcctcgca tgacctggtc acggcagccc tgcaatcggc aggcctgtac
     5341 cagggcagag ccattgtcca gaagtggttg ctggtggccg ttttgggtgc cgcgctggta
     5401 ctcgctttaa caagatggaa cacgaaggct aatgccagac gccgcaccca cgcaggcctg
     5461 gccttggctt gtacggccgc aacatggacg ctgttccttg tggaaacagt atcgctgcat
     5521 gccgtggatc gcgtgctgta tcgaccgatt ggcccggttc ttctgattgc ctatctatgg
     5581 gtaaccaacg ctatggtggt tgtctggctg gcacgacgct cacagctgca ccgccccggg
     5641 ttttgagtca gaatccatgg cttgcgtctc caccctatga tccaactggc aaacaatgcg
     5701 ttacgacgag ccctgaaggg actgatggct gcctgggcca ttctctgggt tttgctgccc
     5761 atgggggctt cagcagcccc ttccccgctg gtgctgtcag acaccacgcc gcaaacggaa
     5821 gtgtggtcgt acgtcaccgt gctgccagag gggggcaaaa aacttggcat ccgtgacgcc
     5881 atgggcgcct tgggccaatt tacaaaaccc gacactgcct atggcacgct ggggctgcaa
     5941 aaggatgccg tctggctgca tatcccggtg tcagtgtccg gcgacggcga cggcctgtgg
     6001 gtacttgaca tcgactaccc cgtcctcaat cgcattgacg tgtacgccgt ccacggccag
     6061 aacatcgtgg tccagacacg tgtgggcagt ctgcaaccac cggaagatcc gcgcgcccgc
     6121 gcccgctcgc atgctgtgag tctggatttg caaccgggcg cccgctacga cctttacatg
     6181 cgcgttgaga gcaaaggttc cctgatcctg cccatgacac tgagcaaacc ctacgcgttt
     6241 ttggtgacgg cactgaaaga gcaaatgctg caggggctac tgaccggact ggccctgtgc
     6301 ctgctggtct acagcctggc gcagtggatc accctgggtg agcacctgta cgtcaagtac
     6361 gcgatcctga ttaccggcag catgctgttt tcgctggtgc aatttggcat aggctcccag
     6421 tacctgtggc caggaaatgc atgggccgag acccatatgg caggactgtc tgcgctggtt
     6481 gctgcgacag gctcctttct gttcattgaa caggctctca aggggcccga tacgctacgg
     6541 tggttcagct ggctcatgaa ggcaggtgct gtgttcaccg ttttttttgc agtgtgcttt
     6601 tcgctggacc tgattgatgt ctacatcgtc accgccgtca tcagctccct gggtctggcg
     6661 ccggccttgc tgggtctccc cggtgctata tcccgggccc gacgcgggga cagtgtgggg
     6721 ttttatttcc tgctggcgtg ggctgtctat tttgtgacca ctgccgtgct catcgaagtg
     6781 atcaaggggc gagtgggcgt gaacttctgg accctgcact ccttccagtt gggggctact
     6841 ttcgacatgc tgattttcat gcgcgtactc ggcctgcgga ccaaggcatt gcaggcggct
     6901 gcccagcatg ccaaacggga acacgaccac ctgcactcca tggcacacac cgaccctttg
     6961 acaggtctgt ccaatcgccg cggcctcaac g
//
LOCUS       DAOSJM010000081         6972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9838_length_6972_cov_3.686136, whole genome shotgun sequence.
ACCESSION   DAOSJM010000081 DAOSJM010000000
VERSION     DAOSJM010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6972
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9838_length_6972_cov_3.686136"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="PKX82_04240"
     CDS             complement(<1..1510)
                     /locus_tag="PKX82_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOF51490.1"
                     /translation="MKSLVAEPNGSAAQLIAATAQSLPEQAGALLRARAFAEPLLASE
                     TLDTGENILAHADAVAAILKTIGGSEAMQAASYLVYACDHLNKPHEVIAKAFGDNFAD
                     LALETTKLVRVQRMARIAQTAASHTAHAAPMAQTAASQTESVRKMLLAFSKDLRVVML
                     RLASRLQTLRHFAASKLPVPPSLASESLQVFAPLANRLGIWEIKWEMEDLSFRFLEPD
                     TYRQVARLLDEKRTEREASVEKLRQQLAADLAAQGITATVQGRPKHIYSIVKKMRGKS
                     LGFEQVYDIRALRIVVPSVPDCYAALSWVHSQFVPVADEFDDYIARPKANGYQSLHTV
                     VRDAAGQPIEVQIRTQAMHDHAEHGVAAHWAYKEAGTKGYAGVSASGEYDAKIAVLRQ
                     LLAWERDLSGQQGAGVFDDRIYVLTPDAAIVELPQGATPVDFAYSVHTNLGHRCRGAR
                     VDGAMVPLNTPLKNGQTVEVTAAKEGGPSRDWLNPELGFLASHRARAKVRAWF"
     gene            complement(1507..2400)
                     /locus_tag="PKX82_04245"
     CDS             complement(1507..2400)
                     /locus_tag="PKX82_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF51491.1"
                     /translation="MTEPTLNYLPCPDATTGHRMAYWQWGHAQAEHTVVCVHGLSRQG
                     RDFDVLAQALMAEAGGNIRVVCPDMVGRGQSDWLADPMGYQLPTYAADMLALLAHLKP
                     RTLDWVGTSMGGLIGMAVCAHAAAVGVTVRRLVLNDVGPVVQWDSIVRIATYLGKSMQ
                     FASLQQAADAMWAISTSFGPHTPAQWLELSRHMVKPLPEGQAGVTLHYDPAIAVPVRA
                     MTQETAAQGEAALWQLFDSITAQTLLLRGAESDLLSRETAAQMTQRGPKARVVEFAGV
                     GHAPTLIADDQVQAVTGFLLE"
     gene            2480..3250
                     /locus_tag="PKX82_04250"
     CDS             2480..3250
                     /locus_tag="PKX82_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HOF51492.1"
                     /translation="MLKGKTALVTGSTSGIGLGIAKALAAQGANIVLNGFGDVDGPKA
                     EIAALGVQVSYHGADMSKPAEIEDMVNFAAKTFGKVDILVNNAGIQHVARIENFPVDR
                     WDAIIAINMSSAFHATRLALPAMRAQNWGRIINVASIHGLVASAEKSAYVAAKHGVVG
                     LTKVTALENATTGVTCNAICPGWVLTPLVQKQVDAKAAALNISNEEATKQLLGEKEPS
                     MQFTTPEELGALAVFFCSPAGNNVRGVAWNMDGGWTAQ"
     gene            complement(3334..5724)
                     /locus_tag="PKX82_04255"
     CDS             complement(3334..5724)
                     /locus_tag="PKX82_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOF51493.1"
                     /translation="MGRFELRKRLGQGAQSTVWLGFDPRMEREVAIKVMRPGDGSDDK
                     AIAQWLQEARSVGRVSHPNIVPVYEADIQDRQPFLVFEYVPGSTLDQVLKQRGALPPP
                     EAVALMMDVLDAVAVAHTAGVIHRDIKPSNILQDASGRARVMDFGIAARIRGGVNDDG
                     AGPGSGTIGYLAPEAANGEISATSMDIFSCGVVLAELLMGKPLLNEADPYRAIYRTVH
                     EQQALPANLSADIDDRLRAIVSRALALDPKQRFASARLFAQELEQWSRPTGAADGEGG
                     KAGGNSTLDFLLRRMRHKSDFPAMSDAVVRIQSMASSDKESVGSVTNEILKDVALTNK
                     LLRLVNSAHYARGGNINTVSRAVNLVGFNGIRNMALSLVLLEHMQDKGQAAQLKEEFL
                     RSLMAGSIAGELCPVLRDSEEAFIGSMFQSLGRLLVQFYFPEEASQIRNLVRSAREPV
                     SEATASLNVLGLSFENLGVGIAKAWGLPDSIQRCMRKPVGAPPPSAPSDTSERIRWVA
                     LASNEIADALLESDPKLVNARIDEIARKYARSIGSTSSAVQAATTKARQKLVDLAQAM
                     EIRVAPQSAASRLLKLPHEVAEGASGTGFEPDATLGGVELHATQTMPLDGGEPPRTKS
                     TMVAETLSAGIQDITNAMVDDFKLTDVLRMILETMYRAMGFDRIIFCMRDPKTETITG
                     RFGLGQGIEKLVPIFKTPLKASTPDLFAVVCLKGVDSLISDATESRIVQRLPEWYRKG
                     INAPTFLLLPLQTKGAPFGLIYADKATHGALELDEKELALLRTLRNQAVMAFKQSS"
     gene            complement(5811..>6972)
                     /locus_tag="PKX82_04260"
     CDS             complement(5811..>6972)
                     /locus_tag="PKX82_04260"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOF51494.1"
                     /translation="TMGHDKGDLLLQQVGQRLMETVREEDTVARLGGDEFVVMFEGLG
                     ATPVEAAARATNLGEKVISILNNAYQLAGAEYHSTPSIGVALFSGTEVGIDDLLKHAD
                     LAMYQAKNAGRNGMRFFDPQMQASVSLRAALEKDIRRGILLGQFLLFYQPQVHADGRV
                     VGAEALLRWAHPERGMVSPADFIPIAEESGHIISLGSWVLEAACAQLLVWAEDPRTEP
                     LTLAVNVSARQFRQPDFAEYVLGLLDYTGANPLRLKLELTESLLADNLEDVIVKMGAL
                     RARGVGFSLDDFGTGYSSLSYLKRLPLDQLKIDQSFVADVLTDPNDASIAQTIVALGR
                     GLGLAVIAEGVETEAQKNFLLQAGCETYQGYLFSKPIPADQFDRYIQSVRPQ"
BASE COUNT         1320 a         2243 c         2134 g         1275 t
ORIGIN      
        1 tgaaccaggc gcgcaccttg gcgcgcgcac ggtggctggc cagaaaaccc agctcggggt
       61 tgagccagtc gcgtgacggc ccgccttcct tggccgcagt cacttccacc gtctggccat
      121 tcttcaaagg cgtgttgagc ggcaccatgg caccgtccac ccgcgcgcca cggcagcggt
      181 ggcccaggtt ggtgtgcacg ctgtaggcaa agtccaccgg ggtagcgccc tggggcaact
      241 ccacaatagc cgcgtcgggc gtgagcacat agatgcggtc gtcaaacacg ccggcgcctt
      301 gctggccgga cagatcgcgc tcccaggcca gcagctggcg cagcaccgca atcttggcgt
      361 cgtattcgcc gctggcagac acccccgcgt agcccttggt gccggcctcc ttgtaggccc
      421 agtgggccgc cacaccgtgt tcggcatggt cgtgcatggc ctgggtgcgg atctgtacct
      481 cgattggctg acccgcagca tcgcgcacca cggtatgcag cgactggtag ccgttggcct
      541 tgggccgggc aatgtagtcg tcgaattcgt ccgctaccgg cacaaactgg ctgtgcaccc
      601 agctgagtgc ggcatagcag tcaggtaccg agggcaccac gatgcgcaga gcccggatgt
      661 catatacctg ctcgaagccc agcgacttgc cacgcatctt cttgacaatg ctgtaaatgt
      721 gtttgggccg gccctgtacc gtggcggtga tcccttgcgc tgcgaggtcc gccgccagct
      781 gctgacgcag cttttccacc gacgcctcgc gctcggtgcg tttttcgtcc agcagacgcg
      841 ctacctggcg gtaggtgtcg ggctccagaa agcggaagga caggtcctcc atctcccact
      901 tgatttccca gatgcccagg cgattggcca gcggcgcaaa cacctgcagc gattcgctcg
      961 ccaggctggg tggcaccggc agcttactgg cggcaaagtg gcgcagggtc tgcaggcgcg
     1021 aagccaggcg cagcatcacc acccgcaggt ctttggagaa cgccagcagc atcttgcgca
     1081 cgctctccgt ttgactggct gctgtttgcg ccatcggcgc ggcgtgggcg gtgtggctgg
     1141 cagccgtctg cgcaatccga gccatgcgct gcacgcgcac cagcttggtg gtttccagcg
     1201 ccaggtcggc aaagttgtcg ccaaaggctt tggcaatcac ctcatgcggc ttgttcaggt
     1261 ggtcacaggc gtagaccaga tagctggccg cctgcatggc ttcggaaccc ccaatggtct
     1321 tgagaatggc agccactgca tcggcgtggg ccaggatgtt ctcgccagtg tccagcgtct
     1381 cgctggccaa caggggctcg gcaaaggcgc gggcgcgcaa tagtgcacca gcctgttcgg
     1441 gcaggctctg ggcagtggcg gcgatcagtt gggccgcgct gccattgggt tccgcaacga
     1501 gacttttcat tcgagcagga agcctgtcac agcttgcacc tggtcgtcgg cgatcagcgt
     1561 gggggcgtgt ccaacaccgg cgaattcgac cacgcgcgct ttggggccac gctgggtcat
     1621 ttgcgcagcg gtttctcgcg agagcaggtc cgattcggcc ccgcgcagca gcagggtttg
     1681 ggcggtgatg ctgtcaaaca gctgccacag cgcggcttcg ccctgcgcgg cagtttcctg
     1741 tgtcatggcg cgcaccggca cggcaatggc ggggtcgtaa tgcagtgtca cgcccgcctg
     1801 cccctcaggc aacggcttga ccatgtggcg agacagctcc agccattggg ctggcgtatg
     1861 cgggccaaaa ctggtggaga tggcccacat ggcatccgcc gcctgctgca gggaggcgaa
     1921 ttgcatggat ttgcccaggt aggtggcgat gcgcacgatg gaatcccact gcaccacggg
     1981 ccccacgtca ttcagcacca aacggcgcac cgtcacgccc acggcagcgg cgtgggcaca
     2041 taccgccatg ccaatgagcc cgcccatgct ggtgccaacc cagtccaggg tgcgcggctt
     2101 caggtgcgcc agcagggcca gcatgtcggc agcgtaggtg ggcagctggt agcccatggg
     2161 gtccgccagc cagtcgctct ggccacgccc aaccatatcg gggcacacca cgcggatgtt
     2221 gccaccggcc tctgccatca gcgcctgcgc cagcacatca aaatcccgcc cctggcggga
     2281 cagcccgtgc acgcacacca cggtgtgctc tgcctgcgca tgcccccact gccagtacgc
     2341 catgcggtgc cccgttgtgg cgtccgggca cggtaagtaa ttcagcgtag gttcagtcat
     2401 ggtgcaatgc aaagatgcgg tgctgtttga taatggcacc atcctactgg aacaaccccc
     2461 actttttgaa agcgacacca tgttgaaagg caaaactgca ctcgtcaccg gttctaccag
     2521 cggcatcggt ctgggcatcg ccaaggcgct ggcggcgcag ggcgccaaca tcgtgctcaa
     2581 tggctttggc gatgtggacg gccccaaggc cgagatcgcc gcgctgggcg tgcaggtgag
     2641 ttaccacggc gccgacatga gcaagcccgc cgagattgaa gacatggtga actttgccgc
     2701 caaaaccttt ggcaaggtag acattctggt gaataacgcc ggcatccagc acgtggcccg
     2761 catcgagaat ttcccggtgg accgctggga cgccatcatc gccatcaaca tgagcagcgc
     2821 cttccatgcc acgcggctgg ccttgcccgc catgcgcgcc cagaactggg gccgcatcat
     2881 taacgtagcc agcatccacg ggctcgtggc ttcggcggag aagtcggcct atgtggcggc
     2941 caagcatggc gtggtgggcc tgaccaaggt gaccgcactg gaaaacgcca ccaccggcgt
     3001 gacctgcaac gccatttgcc ccggctgggt gctgacgccg ctggtgcaaa aacaggtgga
     3061 cgccaaagcc gccgcgctga atatcagcaa cgaagaagcc accaagcagc tgctgggcga
     3121 gaaagagccc tccatgcagt tcaccacgcc cgaagaactg ggcgcgctgg cggtgttctt
     3181 ctgctcaccc gcgggcaaca acgtgcgcgg tgtggcctgg aacatggacg gcggctggac
     3241 ggcccaataa acgcgattta taaccaaatt agcatccagc ccccgtggat agtgcgcaga
     3301 cagctatcta tttgatagca aactacgcgc cactcacgac gactgcttga atgccatcac
     3361 ggcctggttg cgcagggtgc ggagcagggc cagttctttt tcgtccagtt ccagcgcacc
     3421 gtgggtcgcc ttgtcggcgt agatcaggcc aaacggcgcc cccttggtct gcaggggaag
     3481 cagaaggaat gtaggcgcat tgatgccctt gcggtaccac tcgggcaggc gctgcacgat
     3541 gcggctctcg gtcgcgtcac tgatcaggct gtccacaccc ttgaggcaca ccacggcaaa
     3601 caggtccggc gtagaggctt tgagcggcgt cttgaaaatg ggcaccagtt tttcaatccc
     3661 ctgccccaga ccaaagcggc cggtaatggt ctcggtcttc gggtcccgca tgcagaaaat
     3721 gatgcggtca aaccccatcg cgcggtacat ggtctccaga atcatgcgca gcacatcggt
     3781 gagcttgaag tcatccacca tggcattggt gatgtcctgg ataccggcgg acagcgtctc
     3841 ggccaccatc gtgctcttgg tgcgtggcgg ctcgccccca tccagcggca tggtctgcgt
     3901 ggcgtgcaat tccacgccgc ccagcgtggc gtcaggctcg aaacctgtac cggatgcacc
     3961 ctctgccacc tcgtggggca gtttgagcag gcgggaagct gcggattgcg gggcaacccg
     4021 gatttccatg gcctgcgcca gatccaccag cttctggcgc gccttggtgg tagcagcctg
     4081 caccgcgctg gaggtcgaac caatggagcg cgcatatttg cgggcaatct catcgatgcg
     4141 ggcgttgacc agtttggggt cggactccag cagggcgtct gcaatttcat tggacgccag
     4201 ggccacccaa cgaatgcgct ccgaggtgtc ggatggtgca ctgggcggag gcgcccccac
     4261 gggtttgcgc atgcagcgct ggatgctgtc cggcaggccc caggctttgg caatgcccac
     4321 acccaggttt tcaaaactca gacccagcac attgagcgat gcggtcgcct ccgacaccgg
     4381 ctcacgggcc gagcgcacca ggttgcggat ctgggacgcc tcttccggaa agtagaactg
     4441 caccagcagc cgccccaggc tctggaacat ggaaccgata aacgcctcct cgctgtcgcg
     4501 cagcacgggg cacaactctc cagcgataga gccagccatc agcgagcgca aaaactcttc
     4561 cttgagttgg gctgcctggc ccttgtcctg catgtgctcc agcagtacca ggctcagcgc
     4621 catgttgcgg atgccgttga aacccaccag attgaccgcg cgcgacaccg tgttgatgtt
     4681 gccgccacgc gcatagtgcg cgctgttgac cagccgcagt agcttgttgg tcagcgccac
     4741 atccttgagg atttcgttag tgacactgcc cacgctttcc ttgtcagagg aagccatgct
     4801 ctggatgcgg accaccgcat cggacatggc gggaaagtcg ctcttgtggc gcatgcgccg
     4861 cagcagaaag tccagcgtgc tgtttcctcc cgccttgccg ccttccccgt ccgccgcacc
     4921 ggtgggccgg gaccattgct ccagctcctg cgcaaacagc cgtgcactgg caaaccgctg
     4981 tttggggtcc agcgccagcg cgcgggacac aatggcccgc aggcgatcgt cgatatctgc
     5041 gctcagattg gcaggcaagg cctgctgctc atgcaccgtg cggtaaatag cccggtaggg
     5101 atcggcctcg ttgagcagtg gcttacccat cagcaattcg gccagcacca ctccacagga
     5161 aaaaatatcc atggaggtgg ctgaaatctc gccattggcc gcctcgggcg ccagatagcc
     5221 aatcgtgcca ctgccaggac ccgcgccatc atcgttgaca ccgccgcgta tgcgcgcagc
     5281 aatgccaaag tccatcacgc gggcacgccc actggcatcc tgcaggatgt tggacggctt
     5341 gatatcccgg tggatgaccc cggcggtgtg tgctaccgct acggcgtcga gcacatccat
     5401 catcaaggcg acggcctccg gcggaggcag tgcaccccgc tgtttgagca cctggtccag
     5461 cgtactgcca ggcacgtact cgaacaccag gaagggctgg cggtcctgga tatctgcctc
     5521 gtagacagga acaatgttgg ggtgactcac gcgcccgacg ctgcgcgctt cctgcagcca
     5581 ctgggctatc gccttgtcgt cggagccgtc gcccgggcgc atcaccttga tggcgacttc
     5641 gcgctccatg cgcggatcga accccagcca gacggtggac tgggcgccct gccccagccg
     5701 tttgcgcaat tcgaaacgtc ccatcgtggc gggagaacga ccgctctgga gagcggcacc
     5761 tggattccgg acggaattgg aaaaatcggc catcgttttt gtcggtagat ttattgtggt
     5821 cgaacagact ggatatatcg gtcaaactgg tcagcaggta taggcttgct gaaaagatag
     5881 ccttggtagg tttcgcatcc tgcctgcaaa aggaagtttt tctgggcctc ggtttccacg
     5941 ccttcggcaa tgaccgccag accgagcccc cgccccagcg ccacaatggt ctgggcaatg
     6001 gatgcgtcgt tcgggtcggt cagcacatcg gccacaaaag actggtcgat cttgagctgg
     6061 tccagcggca agcgcttgag gtagctcagt gaggagtatc cggtaccaaa atcgtccagc
     6121 gagaagccca cgccacgcgc acgcagagca cccatcttga cgatcacgtc ttccagattg
     6181 tcggccagga ggctctcggt cagctccagc ttcaggcgca gcggattggc cccggtgtag
     6241 tccagcaggc ccagcacgta ttcggcaaag tcaggctggc ggaactgccg ggcactgaca
     6301 ttgaccgcca gcgtcagagg ctcggtccgt ggatcttcgg cccacaccag cagttgggca
     6361 catgccgctt cgagcaccca gctacccaaa gaaataatat gcccgctctc ttcggcaatc
     6421 ggaatgaaat ccgccgggga caccataccc cgctccgggt gcgcccagcg caacagcgcc
     6481 tcggcaccga ccacacgccc atcggcatga acctgcggtt ggtaaaacag caaaaactgg
     6541 cccagcagaa taccccgacg gatatctttc tccagggcgg cacgcagtga tacagaagcc
     6601 tgcatctgcg gatcgaagaa acgcatgccg ttgcgcccgg cattcttggc ctggtacatc
     6661 gccagatccg catgcttgag caggtcatca atgccgacct cagtgccgct gaacagcgcg
     6721 acaccaatac tcggtgtact gtggtattcc gcaccggcga gctggtaggc attgttgagt
     6781 atggaaatga ccttttcacc caggttggtg gccctggcag ccgcttcgac gggcgtcgcc
     6841 ccaagccctt cgaacatgac cacaaactcg tccccaccca gccgggcgac ggtatcttct
     6901 tcgcgcaccg tctccataag tcgctgcccg acctgctgca gcagaagatc ccctttgtca
     6961 tgccccatgg tg
//
LOCUS       DAOSJM010000082         6969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9846_length_6969_cov_4.917125, whole genome shotgun sequence.
ACCESSION   DAOSJM010000082 DAOSJM010000000
VERSION     DAOSJM010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9846_length_6969_cov_4.917125"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..742
                     /locus_tag="PKX82_04265"
     CDS             218..742
                     /locus_tag="PKX82_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HOF51495.1"
                     /translation="MLIIIVGAVLVLALGGGGAFFYISKQRAAAEEGEDGAPAKTAAK
                     DAHKAPPAYLPLDNMVVNLADPGGERVAQVGITLELLDAKSAETVKAYLPTIRSGVLM
                     LISQRTAEELLKPEGKEKLAKDILRESSLPFGGGDEEHEDESAAPKKTAKKKKIVHPE
                     YPVVGVLFSSFIVQ"
     gene            763..1767
                     /gene="fliM"
                     /locus_tag="PKX82_04270"
     CDS             763..1767
                     /gene="fliM"
                     /locus_tag="PKX82_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOF51496.1"
                     /translation="MSESFLSQEEVDALLEGVTGESQKSVEEVAEHGEVRSYNISSQE
                     RIVRGRMPTMEIVNERFARNLRVGLFNFIRRSPEISVGPVSVQRYSAFLRELAVPTNF
                     NIVAIRPLRGSGLIVCEPALVFGVIDTLYGGIGKFQTRIEGRDFSATEQRVINRMVDV
                     ITEEYKKAWKGIYPLELEYQRSEMQPQFANIATPSEIVISTSFQLEIGDIAGSIHFCF
                     PYSTLEPIRDVLYSSTQGDSMEVDRRWVNVLTREIQAAEVTLVAELARANATVEQLMA
                     MKKGDFIELERAPKIQAAIDGVPIFECHYGTHNSKYAIRIDKALRGNDRNWLGEKNGI
                     "
     gene            1757..2197
                     /gene="fliN"
                     /locus_tag="PKX82_04275"
     CDS             1757..2197
                     /gene="fliN"
                     /locus_tag="PKX82_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOF51497.1"
                     /translation="MASEDKPDEAAADDGMADWAEALLEQKATDSAAEAPGGVLSGEA
                     PKPFSSGSSSDGPMSDINMVLDIPVQLSVELGRTKVPIKHILQLGQGSVVELDALAGE
                     PMDVLVNGYLIAQGEVVVVNDKFGIRLTDVVTPSERLRRVSKNG"
     gene            2204..2485
                     /locus_tag="PKX82_04280"
     CDS             2204..2485
                     /locus_tag="PKX82_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOF51498.1"
                     /translation="MTQSLVSIAIFLGLLACLPMAVKWLKTRSGYTPLPVGDQAVVVS
                     AVAVGPHQRVVTVEVGPQDARVRLTLGVTAQSITCLHTAPLVQSTVVPA"
     gene            2524..3264
                     /gene="fliP"
                     /locus_tag="PKX82_04285"
     CDS             2524..3264
                     /gene="fliP"
                     /locus_tag="PKX82_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596635.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOF51499.1"
                     /translation="MAGRVLAGLFFLLLGDAVLAQTPGQLPLLVGTGGSGTSFSVPIQ
                     TLLFFTTLSFLPAILLMMTGFTRIVIVLSLLRQALGTQSSPPNQVIVGLSLFLTLFVM
                     GPTLDKIYAEAYVPYTASKISFETALEKAEGPIRGFMVKQTRQSDFALFAKLAKLPAD
                     VTAETAPLRVLVPSFVISELKSAFQIGFMIFIPFLVIDIVVASVLMSLGMMMLSPVLV
                     ALPFKLMLFVLADGWNLLIGSLAASFVT"
     gene            3279..3548
                     /gene="fliQ"
                     /locus_tag="PKX82_04290"
     CDS             3279..3548
                     /gene="fliQ"
                     /locus_tag="PKX82_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOF51500.1"
                     /translation="MNSQMVLTMGQEALLMLLMVSSPVLGVALVVGLLVSLFQAVTQI
                     HEATLAFVPKLIAVIAVFAIAGPWMLTMLVEYIRRTIEAIPASVV"
     gene            3565..4326
                     /gene="fliR"
                     /locus_tag="PKX82_04295"
     CDS             3565..4326
                     /gene="fliR"
                     /locus_tag="PKX82_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOF51501.1"
                     /translation="MVTFTEAQLVAWVSPILWPFLRVLAVFTSAPVFSSRAFPVRARI
                     ALAFLVAYASQSTLPAAPLIGFNDPGALGVVVQQVGIGLAIGFAVRVVFAAVELAGEV
                     VGFQMGLNFAAFFDPSLNSQSSAVARFFGQMASLLFVVMNGHLMVLMAVNKSFEAFPI
                     DQNFLEALGKMKLYQLGSDLFASALWIALPMVGMLMFINLALGVISRVAPQMNIFAVG
                     FPITLVVGLIGIAYTLPMLDQPFLALMGRVIDIFG"
     gene            complement(4338..4967)
                     /locus_tag="PKX82_04300"
     CDS             complement(4338..4967)
                     /locus_tag="PKX82_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF51502.1"
                     /translation="MIRVVLCDDHAMVRRGIRDTLSEAVDIQITGESASYTEVREVLR
                     STPCDVLVLDLNLPGRGGMEVLASLRESDSPVRVLIVSMFAEDQYAIRCLRAGAHGYL
                     NKAGDPADLINAVRAIAQGRKFVTPAVSELLVDSLATPGTEALHSTLSEREMQTLLKI
                     ASGKKLSDIAEELMLSPKTVSVYRSRLLEKLKLSNNAELTVYAIRNELV"
     gene            complement(4964..6091)
                     /locus_tag="PKX82_04305"
     CDS             complement(4964..6091)
                     /locus_tag="PKX82_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOF51503.1"
                     /translation="MIASNASPGFKELPLRILHLEDSRLDHELVCRELAKGGTPFVIE
                     RVETLPEFVSVTSQRTFDVVLADFRLPGFTALDAWNAISSHNPHLPFILLSGAIGEAA
                     AVNAIQTGVSDYLHKDELSKLWRVILRALEVSQVKVAEEKANRELKLSEKRLAQFAEH
                     LQSTIENERADIAREIHDDIGGALAAVKLDLAWLSRHTTAGEARGHIDTAQEMLQHAL
                     GASQRIMMNLRPAILDQGLYPALQWLVRNFEKRTGIHSTMAINHEALKLERAVELAAY
                     RTTQEALTNISKHADCTAVRIELSDAEGVLTIDISDNGKGISSPDREKPKAFGLRGLQ
                     ERAKSVGGWLDISSTPGKGTSIILSVPLAGNTRQHEEELFQ"
     gene            6264..6707
                     /locus_tag="PKX82_04310"
     CDS             6264..6707
                     /locus_tag="PKX82_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51504.1"
                     /translation="MESSLTTPSAPSVQMPVMLVAEVQDSLLVVVHDLTRLDGLLAHA
                     MENLMERFTSASASLADPELVSSAELDSVRSTLRAAVTELQFQDMASQLITHTSKILQ
                     GCAYRLASESMGSEDGEAVPFVEEVPERPNPVTQDEMDAGSIELF"
     gene            6748..>6969
                     /locus_tag="PKX82_04315"
     CDS             6748..>6969
                     /locus_tag="PKX82_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF51505.1"
                     /translation="MSVILAVDDSPSMRKMVSFTLTGAGYQVVEAVDGQDAYEKAMSQ
                     SFDLVLTDQNMPRLDGLGLTRKLRDHPQFK"
BASE COUNT         1315 a         1813 c         2143 g         1698 t
ORIGIN      
        1 tatgccggca ggtgccgcag gacggacgct ggacctgttt gtctagggtg cgagaaatgt
       61 gtcaatatca gcgtgagacc gcaaattccc cgcagtttct gccctttgcc gtcgccagaa
      121 cccgccaata atcaagctag cttgaagtta aggaaccacc gtgtcagaca aaccagccgc
      181 cgaagaaggg gataagccca aagccaagag caagaagatg ctcatcatca tcgtgggtgc
      241 ggtgctggtt ctcgcattgg gcggtggcgg ggccttcttc tatatcagca agcagcgcgc
      301 cgccgcggaa gagggcgagg atggcgcacc agccaagacg gctgccaagg atgcgcacaa
      361 ggcaccccct gcgtacctgc ccctggataa catggtggtc aaccttgcag accccggtgg
      421 cgagcgcgtc gcccaggtcg gaatcaccct ggaactgctg gacgccaagt cggcagaaac
      481 cgtcaaagcc tatctaccga ccatccgcag cggggtcctg atgctgattt cgcaacggac
      541 ggccgaggag ttgctcaagc ccgaaggcaa ggaaaagctg gccaaggaca ttctgcgtga
      601 atccagcctg cctttcggag gcggggatga ggagcacgaa gacgagagcg ccgctcccaa
      661 gaagacggcg aaaaagaaga agattgtgca cccggagtac cccgtggtcg gtgtgctgtt
      721 ttccagcttt attgttcagt aagctgttgt gagaattgtc caatgagcga gtcgtttctt
      781 tcccaggaag aagttgacgc ccttctcgaa ggggtcacgg gcgagagcca gaagtccgtc
      841 gaagaggtgg cggaacatgg cgaagttcgt tcgtacaaca tttccagcca ggagcggatc
      901 gtccgtgggc gcatgcccac gatggaaatc gtcaacgagc ggtttgcgcg caacctgcgg
      961 gtaggcctgt tcaacttcat ccggcgcagt ccggagattt cggtcggacc ggtttccgtg
     1021 cagcgctaca gcgccttcct gcgtgagctg gcagtgccga ccaatttcaa tatcgtagcc
     1081 atccggcccc tgcgcggcag cgggttgatt gtgtgcgagc cggcactggt gttcggtgtg
     1141 attgacacgc tgtacggcgg tattggcaag ttccagaccc gtattgaggg acgtgatttt
     1201 tctgctaccg agcagcgcgt gatcaaccgc atggtggatg tgatcaccga ggaatacaag
     1261 aaggcctgga agggtatcta tccgctggag ctggaatacc agcgttcgga aatgcagccg
     1321 caatttgcga acattgccac gccgagcgag attgtcattt caacgtcctt ccagctggaa
     1381 atcggggata ttgcaggatc tatccatttc tgttttccgt actccacgct ggagcctatc
     1441 cgcgatgtgc tctactcgtc cacgcagggc gattccatgg aagtggaccg gcgctgggtg
     1501 aatgtgctga cccgtgaaat tcaggcggcc gaagtgaccc tggtggcgga gctggcccgt
     1561 gccaatgcca ccgtggagca gctgatggcg atgaaaaagg gtgattttat tgagctggag
     1621 cgtgcgccca agatccaggc ggccatcgat ggggttccca tctttgagtg ccactatgga
     1681 acgcacaatt ccaaatacgc catacgcata gacaaggcct tgcgtggcaa tgaccgaaac
     1741 tggttgggag aaaagaatgg catctgaaga taaacctgac gaagccgctg cagacgacgg
     1801 tatggcggac tgggcggaag ccctgctgga gcaaaaggcg accgatagcg cggcggaagc
     1861 gcccgggggc gtcctgtcgg gagaggctcc caagcccttt tcttccggca gctcatccga
     1921 tggtcctatg agcgacatca acatggtgct ggacattcca gtccagttgt cggtggagtt
     1981 aggcaggacc aaggtgccca tcaagcacat cttgcagctg ggacagggtt cggtggtgga
     2041 gctggatgcc ctggctggcg agcccatgga tgtgctggtc aatggctacc tgatcgcgca
     2101 aggcgaagtc gtggtggtga acgacaagtt tggtattcgt ctgaccgacg tggtgacgcc
     2161 gtccgagcgc ttgcgcaggg tcagcaagaa tgggtaactg gcgatgacgc agtccctggt
     2221 atcgattgcc atcttcctgg ggttgctggc ctgcctgccg atggcggtca aatggctaaa
     2281 gacgcggtct ggttatacgc cactgccggt gggtgaccag gcggtcgtgg tctccgccgt
     2341 agccgtgggg ccgcaccaac gagtggtcac ggtggaggtt gggccgcagg atgcgcgtgt
     2401 gcggctgacg ctgggcgtga cggcccagtc cattacctgc ttgcatacgg cgcctctggt
     2461 gcagtccact gtggtgccgg catgacggct ggcgattgtg gacaacaagt agatcggagc
     2521 gttatggcgg gtcgcgtcct tgcggggctt tttttcctgt tgctggggga tgcggtgctg
     2581 gcgcaaacgc ccggacagtt gccgttgctg gtcggtactg gcgggtctgg aacgagtttt
     2641 tccgtgccca ttcagacact gctgtttttt accacgctgt cgttcctgcc cgccattctt
     2701 ttgatgatga cgggttttac ccgcatcgtc atcgtgctgt ccctgttgcg gcaggcgctg
     2761 ggtacacaat cctcgcctcc gaaccaggtc attgtgggcc tgtcgctgtt tctgacgctt
     2821 ttcgtcatgg ggcctacgct cgacaagata tacgctgaag cgtacgttcc ttataccgcc
     2881 agcaagatct cttttgagac cgctctcgaa aaggcggaag gtcccatacg cggattcatg
     2941 gtcaagcaga cgcgtcagtc ggacttcgcc ctgtttgcaa agctggccaa gctcccagcc
     3001 gatgtgacgg ctgaaaccgc accgctgcgt gttctggtgc cgtcctttgt catcagcgag
     3061 ctcaagtcgg cgtttcagat cggcttcatg attttcatcc ccttccttgt gatcgatatc
     3121 gtggttgcca gtgtgttgat gtcgctgggg atgatgatgc tgtcgccggt gctggtggcg
     3181 ttgccgttca agctcatgct gtttgtgctg gccgatggct ggaatctgct gattggctcg
     3241 ctggcggcca gttttgtcac ttagaagggc ttcattgcat gaactcacag atggtgctga
     3301 ccatgggcca ggaggctctg ctgatgctat tgatggtgtc gtctcctgtg ctgggtgtcg
     3361 ccctggtggt ggggctgctg gtcagcctgt ttcaggcggt gacccagatc catgaggcga
     3421 cactggcttt tgtgcccaag ctgatcgcag tcattgcggt ttttgcgatt gccgggccat
     3481 ggatgttgac catgctggtg gaatacattc gccgcactat tgaagccatt cctgcttcgg
     3541 tcgtgtgatt cgccgggcca ggatgtggtg actttcacag aggcgcagtt ggtggcatgg
     3601 gtttctccca tcctctggcc ttttttgcgt gtgctggcag tttttacatc ggcaccagtg
     3661 ttctcttcac gggcttttcc ggtgcgcgcg cgtatcgcgc tggcttttct ggtggcctat
     3721 gcatcgcaaa gtaccttgcc tgcggcgcca ctgattggat tcaatgatcc tggcgcactg
     3781 ggtgtggtgg tgcagcaagt gggcattggt ctggcgattg gcttcgctgt acgcgtggtg
     3841 tttgccgcgg tcgaactggc gggcgaagtc gtgggctttc agatgggact gaatttcgcc
     3901 gccttttttg acccctcgct gaattcccag tccagtgccg tggcccgctt cttcggtcaa
     3961 atggcttcgc tgctgtttgt ggtgatgaat gggcacctga tggtgttgat ggcggtcaac
     4021 aagagttttg aggcttttcc catcgatcag aattttctgg aggcgttggg gaagatgaag
     4081 ttgtaccaac tgggctcaga cctctttgcc agtgcactct ggattgcctt gccgatggtg
     4141 ggcatgctga tgttcatcaa ccttgcgctg ggggtgattt cccgggtggc tccgcaaatg
     4201 aacatttttg ccgtggggtt ccctattacc ctggtggtcg gtctaatcgg aattgcctac
     4261 acgttgccca tgctggacca gccatttctg gctttgatgg ggcgtgtcat cgatatattt
     4321 gggtgatctg gcggtagtta caccagttca tttctgatgg cgtacaccgt cagttcggca
     4381 ttgttggaca gtttgagttt ttccagcaac cgggatcggt acacgctcac tgtcttgggg
     4441 ctcagcatca actcttcggc aatgtcggag agcttcttgc cggatgcaat tttcagcagc
     4501 gtctgcattt cccgctcgga cagtgtggag tgcagcgctt ccgtacccgg tgtagcaagg
     4561 ctgtccacca gcaattcgga cactgcgggt gtcacgaatt tacggccttg ggcgatagcc
     4621 cgtaccgcgt tgatgaggtc cgcaggatcg ccagccttgt tgaggtagcc atgcgcgcca
     4681 gcgcgcaggc accggatggc gtactggtct tcggcaaaca tggaaacgat cagcacccga
     4741 acgggagaat cggattcgcg caggctggca agtacttcca tgccgccgcg ccccggcaga
     4801 ttcaggtcga ggaccagtac atcgcagggt gtactgcgca gcacttcccg aacttccgtg
     4861 tagcttgcgg attctcccgt gatctggatg tcgaccgctt cggacagcgt gtcgcggatc
     4921 ccccttcgga ccatcgcgtg gtcatcgcac agtacgacgc gtatcactgg aacagttcct
     4981 cttcatgttg ccgtgtgttg ccggccaggg gcacagacag gatgatggat gtgcctttgc
     5041 cgggggttga gctgatgtcg agccagccgc cgacgctttt tgcgcgttcc tgcagtcccc
     5101 gaagaccaaa ggccttgggc ttttcacggt cgggactcga aatgcctttg ccgttgtccg
     5161 aaatatcaat ggtcaaaacc ccttcggcat cggatagttc gatcctgacg gcagtgcagt
     5221 cggcatgttt ggagatgttg gtcagggcct cttgcgtagt gcggtaggca gccagttcca
     5281 cagctctttc cagtttgagg gcttcatggt tgatcgccat ggttgagtgg atgcccgtac
     5341 gtttttcgaa gtttctcacc agccactgga gtgccgggta cagcccctga tcaagtatgg
     5401 cgggacgcaa gttcatcatg atgcgctggc ttgcgcccag tgcgtgctgg agcatttcct
     5461 gggcagtgtc gatgtgccct cgcgcctcgc cggctgtggt gtgtcgggac agccatgcca
     5521 agtccagttt gaccgctgcc agggctccgc caatgtcatc atggatttca cgtgcaatgt
     5581 cggcgcgctc gttttcgatg gttgactgca gatgctctgc gaattgggcc aggcgctttt
     5641 ctgagagctt cagctcccga ttggcctttt cctctgccac tttgacttga ctcacctcca
     5701 gtgcgcgcaa tattacccgc cagagtttgc tcaattcgtc tttgtgcaga tagtcgctga
     5761 cgcctgtttg aatggcgttg accgctgctg cctcaccgat ggccccggaa agcaggatga
     5821 acggtaggtg agggttgtgg ctggaaatgg cgttccacgc atcgagcgcg gtgaaaccgg
     5881 gcagccggaa atcagccagg acgacgtcaa atgtgcgttg tgatgtgact gacacgaatt
     5941 caggcaatgt ttcaacgcgc tctatgacaa atggcgttcc acctttggcg agttcacggc
     6001 ataccaattc gtggtccaga cgcgaatctt ccaaatgcag aattcgcagc ggcaattctt
     6061 tgaatcctgg ggatgcgttc gaagctatca tcgcgagaat tatggaccgt gctgaatctg
     6121 gattccaata tgcttggcgg aagttggcca attcaacggg tgttttttcg acaaggctga
     6181 agtttcctgc taatcttgtg gcgagcgcac tttgcaccgg atgaaatgtt tgggatatat
     6241 gtgaatttct gggacgcatg acaatggaat catctttgac aacgccatct gcaccttcgg
     6301 tgcagatgcc agtcatgctc gttgctgagg ttcaggactc cttgttggtc gttgtgcatg
     6361 acctgacccg tctggatgga ttgcttgcgc atgccatgga aaacctcatg gagcgattta
     6421 catctgcgag tgccagtctc gccgatcccg agcttgtcag ttctgccgag ttggatagtg
     6481 tgcgtagcac gttgcgcgca gcggtgactg agttgcagtt ccaggatatg gcgtcacaac
     6541 tcattacgca cacttccaag atactgcagg gatgcgcata caggctggcg tctgaatcta
     6601 tggggtctga agatggtgag gcggttcctt ttgtcgaaga ggtccctgag cgccccaatc
     6661 cagtcaccca ggatgagatg gatgccggct caattgagct tttttgatga ttttcgcaac
     6721 ctcaatttga atgagatgga gttctaaatg tctgtaattc tcgctgttga tgactcgcca
     6781 tcaatgcgca aaatggtctc tttcacgttg actggggctg gatatcaggt ggtggaagcg
     6841 gtagatgggc aggacgctta cgagaaggct atgtcgcagt cgtttgatct ggttttgacg
     6901 gaccagaata tgccccgtct tgatggcctg gggctgacgc gtaagttgcg cgatcatcct
     6961 cagtttaag
//
LOCUS       DAOSJM010000083         6963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9858_length_6963_cov_4.115808, whole genome shotgun sequence.
ACCESSION   DAOSJM010000083 DAOSJM010000000
VERSION     DAOSJM010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9858_length_6963_cov_4.115808"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PKX82_04320"
     CDS             complement(<1..443)
                     /locus_tag="PKX82_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF51506.1"
                     /translation="MTTIEPVLRISGLTKHYGGVKALTDAHFQLLPGEHAAIVGDNGA
                     GKSTFVRLITGAEQPNDGDISLDGQKVNFESPLDAREQGIETVYQTLALAEDLDVPAN
                     IFLGRELTRLNLGPLSILNHKAMREQSATMLSTTGVKIQDMSESLR"
     gene            614..1570
                     /locus_tag="PKX82_04325"
     CDS             614..1570
                     /locus_tag="PKX82_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOF51507.1"
                     /translation="MSMKRMIGRLAIAMAAAGMMTAALADTTIAYITNGNTNEGWTLI
                     NGGAKKAGQKPGVKFIELAAEKGELSKQLAIVEDMITRKVNAIAIAPVDSKGIAPAIN
                     KALAAGIKVVAVDTGITGAAITSYVATDNIKAAMVQGNWAAEQVKDGDTVIYITGDLG
                     QSTGQERKKGFLDGLNAKRKNVKVVEVPTTWDQTMAQNGVETALRANPTAKVIACAWD
                     GGALGAKAALMAAGKKAGDVKIAGFDGSPGGLDMMKQGWQSANAAQMLAKIGQVGVET
                     AIAAAEGKKVDARIDTGSFLVLPSNVDQFAKDSGVAQFMKVK"
     gene            1644..2588
                     /locus_tag="PKX82_04330"
     CDS             1644..2588
                     /locus_tag="PKX82_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51508.1"
                     /translation="MKSITRQEWYGALVAVMVLGLLLTFASPYFLTTGNLSNILVQAS
                     VIALLAGGQTFVILTGGVDLAVGALTALAGAVAGHMMIKLGVDPYVAIAAALAIGAAV
                     GVFNGYLVAFVGIPSFIVTLGGLTLWRGLAFESTGGFDNSGMPDPLPFIGYGQLLGVP
                     MPIWITGIFFVVMAFVLSSTKLGRYVYAMGSNEMGARQVGINIRWYKLGVYVVSGLSC
                     AIGAVVLMARMDSSSGKMAQMFELDAIAAVILGGTSLFGGRGSIWGSLLGAVLITMIR
                     NGMNLMEISQFKQMMAIGAVVIVAVWIDVLRRQHLLKR"
     gene            2585..3514
                     /locus_tag="PKX82_04335"
     CDS             2585..3514
                     /locus_tag="PKX82_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOF51509.1"
                     /translation="MSVAIYVLGEALMDCVAQPDGSLRPYMGGSPFNLARAAALRGAQ
                     VHFLNPFSTDRFGAQMVAQLTRDTVLCTQPPSRLPTSLAVVQVTDGQPSYGFYREGIA
                     DRDYTVSQVLEALHKVPPGVLHTGSLMLIPPEHHKVLELLQGAKAMGWKISVDVNLRP
                     QLAEDLDAYLAAVHAVAALADWIKASDEDLELLGFPVATRAQASEIARFFQDQGATRV
                     ALTFGAQGAWLAVDGVTAEADAPGITLVDSVGAGDTFWGNCLAEWTLELDHAPQRVAR
                     TLQLAMAAAAINCMRAGCQPPTSAELLAATTLA"
     gene            complement(3530..4645)
                     /locus_tag="PKX82_04340"
     CDS             complement(3530..4645)
                     /locus_tag="PKX82_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOF51510.1"
                     /translation="MSIAIRNVSKEFGTFKALNDVSLDIESGELVALLGPSGCGKTTL
                     LRIIAGLETADQGNILFSGEDTTDVHVRERNVGFVFQHYALFRHMTVFDNVAFGLRMK
                     PRATRPSEIVIKQKVHDLLGLVQLDWLADRYPAQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRRLHDDLHVTSIFVTHDQEEALEVADRVVLMNSGQVEQI
                     GSPQEVWDHPASPFVYGFLGDVNLFNGRAHEGEMRIGEDHHGVRLSSPEHSGAQDAKA
                     FAYVRPHDLDVRRYEAGEDNHLNGYQQQRGIPAKLVRAIVVGPIARLELLPLDASAAG
                     SGDIIEAQIGAQQFREQAYKEGEVLVLTPRKARVFVA"
     gene            complement(4664..5647)
                     /gene="cysW"
                     /locus_tag="PKX82_04345"
     CDS             complement(4664..5647)
                     /gene="cysW"
                     /locus_tag="PKX82_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004531228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOF51511.1"
                     /translation="MSNSRPNTAGPSQGVSAPSGSSAVRAATSVGARDVRRAKAGTTE
                     PAWVKWTLIGLALTFIFLFLVLPLAAVFTEALRKGADAFFEAFKEPDAWSAIRLTLIT
                     AAIAVPLNLVFGVAAAWAIAKYEFRGKAFLTTLVDLPFSVSPVVAGLIYVLMFGAHGW
                     FGPWLQAHDIKIVFAVPGIVLATIFVTFPFIARELIPLMQAQGTDEEQAAQVLGASGW
                     QTFWHVTLPNIKWGLIYGVILCNARAMGEFGAVSVVSGHIRGQTNTMPLHVEILYNEY
                     QSVAAFAIASLLALLALVTLVIKSFVEWQFERDQKAAAALPPERPLAPVLP"
     gene            complement(5650..6486)
                     /gene="cysT"
                     /locus_tag="PKX82_04350"
     CDS             complement(5650..6486)
                     /gene="cysT"
                     /locus_tag="PKX82_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOF51512.1"
                     /translation="MTVAKRAPKRVLPGFRLTLGFTLFYLALIVLIPLSALVFKTFTL
                     SWEQFVSAVSSPRVIASYQLTFGASFLAALVNAVFGLLVAWVLVRYQFPGKKIVDALV
                     DLPFALPTAVAGISLTALLASNGWVGSILEPYGITLAFNRNGVVIALIFIGLPFVVRT
                     VQPVLEDAEKELEEAATCLGATRWQTFRYVIFPTIGPALLTGFAMAFARGVGEYGSVI
                     FIAGNMPMLSEITPLLIIGKLEQYDYAGATAVATVMLLASFFMLLVINGLQSWQRKRS
                     GA"
     gene            complement(6487..>6963)
                     /locus_tag="PKX82_04355"
     CDS             complement(6487..>6963)
                     /locus_tag="PKX82_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF51513.1"
                     /translation="QAAEFVSKLYKNVPVLAKGGRDATSIFLQRNIGDVLITFESEVV
                     SVDKEFGAGKVDAIHPSSSIVAENPVAVVERTVAKKGTVEQAKAYLNFLYSDEGQEIA
                     AKHAIRPTNPAILKKYASTFKPIALFRAEDYFGSFAEAQKVHFNDGGQFDKLYTVK"
BASE COUNT         1360 a         2083 c         2200 g         1320 t
ORIGIN      
        1 cgcaggctct cggacatgtc ctggatcttc acacccgtgg tggagagcat ggtggcagat
       61 tgctcacgca tggccttgtg gttgaggatg gacaaagggc ccaggttcag gcgggtgagc
      121 tcccggccca gaaagatgtt ggcaggcaca tccaggtctt cggcgagcgc aagcgtctgg
      181 tagacggttt cgatgccttg ctcgcgcgca tccagtggcg actcaaagtt gaccttctgg
      241 ccgtccaggc tgatgtcgcc gtcgttgggc tgctcggccc cggtaatcag gcggacaaac
      301 gtgcttttac cggcgccgtt gtcgcccacg atggctgcgt gctcaccggg caggagctgg
      361 aaatgggcgt ccgtcagggc tttgacgccg ccgtaatgct tggtcaggcc gctgatgcgc
      421 agcacgggct caatggttgt catggcagtc tctttcgcaa aagtagcagc aaggatggca
      481 caggttcggg cgcaatcata ttagtaaaac tactaggttt atttagtcgg aaagtctgct
      541 gtaatccatt cccagcaagc aatggggcac ttccagcgct gctaacctca acttatatat
      601 tggagatatt cctatgagca tgaagcgcat gatcggccgc ctggccattg caatggccgc
      661 cgcaggcatg atgaccgccg ccctggcgga caccaccatt gcctacatca ccaacggaaa
      721 taccaacgaa ggctggaccc tgatcaacgg cggcgccaag aaggcaggcc agaagcccgg
      781 cgtcaaattc atcgaactgg ccgccgaaaa gggcgagctg tccaagcaac tggctatcgt
      841 ggaagacatg attacccgca aggtcaacgc tatcgccatc gccccggtcg atagcaaggg
      901 cattgcaccg gccatcaaca aggcgctggc cgcgggcatc aaggtggtgg ccgtagatac
      961 cggcatcacc ggcgcagcga tcacttctta tgtggcgacc gacaacatca aggccgccat
     1021 ggtgcagggc aactgggccg cagaacaggt caaagacggc gacacggtga tctacatcac
     1081 tggtgatctg ggccaatcca cgggccagga gcgcaaaaag ggcttcctcg acggcctgaa
     1141 tgccaagcgc aagaatgtca aagtggtgga ggttcccaca acctgggacc agaccatggc
     1201 gcaaaacggt gtggaaaccg cactgcgtgc caatcccaca gccaaggtga ttgcctgcgc
     1261 atgggacggt ggtgcactgg gtgccaaggc tgccctgatg gccgctggca aaaaggcggg
     1321 tgatgtgaag attgccggct ttgacggctc tcccggcggt ctggacatga tgaagcaagg
     1381 ctggcaaagc gctaacgccg cgcaaatgct ggcaaaaatc ggccaagtgg gcgtagaaac
     1441 cgctatcgcc gctgctgaag gcaaaaaagt ggatgcccgc atcgacaccg gctccttcct
     1501 tgttctgccc tccaatgtgg accagtttgc caaggactcg ggtgttgccc agttcatgaa
     1561 ggtgaagtaa tccctggcca atgccagaaa acagggagcg gtggccgcgt ggttgccgct
     1621 cccctgattg aggaaaagaa ttcatgaagt ccattacccg gcaagagtgg tacggcgcac
     1681 tggtggccgt tatggtcctg gggctgttgc tgaccttcgc atccccctac tttctgacca
     1741 ccggcaacct gtccaatatc ctggtccagg cgtctgtgat tgccctgctc gcgggtggac
     1801 aaacctttgt catcctgacc ggtggcgtcg acctggccgt cggggcattg actgctctgg
     1861 ccggggcagt ggctggccac atgatgatca aactgggggt ggacccgtat gttgccatcg
     1921 cggcagccct cgccattggc gccgctgtgg gcgtatttaa cggttatctg gtcgcatttg
     1981 tcggcattcc gtcgttcatt gttactttgg gtggcctgac gctgtggcgg ggcctggcct
     2041 tcgaatccac tggaggattt gacaactcag gcatgccaga ccccttgccg ttcattgggt
     2101 acgggcagtt gctgggtgtg cccatgccca tctggattac cggcatcttc tttgtcgtga
     2161 tggcttttgt cctgtccagc accaagctgg gccgctacgt gtacgccatg ggctcgaatg
     2221 aaatgggtgc ccgccaggtg ggtatcaaca tccgctggta caagctgggc gtctatgtgg
     2281 tgtcgggact gagctgcgcc ataggagccg tcgtgctgat ggcgcgtatg gactccagca
     2341 gcggcaagat ggcgcaaatg tttgaactcg acgccattgc cgccgtcatt ctgggcggta
     2401 cctcactgtt tggcggtcgc ggcagcatct ggggcagcct gttgggggcg gtgctgatca
     2461 ccatgatccg caacggcatg aacctgatgg agatttcgca gttcaagcag atgatggcca
     2521 ttggtgccgt ggtcatcgtg gccgtgtgga ttgatgtcct gcgccgccag cacctgctga
     2581 aacgatgagc gttgccatct acgtgttggg cgaagccctg atggactgcg ttgcgcagcc
     2641 agacgggtcg ctgcgcccct acatgggcgg gagccctttc aacctggcgc gcgctgcggc
     2701 cctgcgcggc gcgcaggtgc attttctgaa tccgttttcc acagaccgtt tcggggcgca
     2761 gatggtcgcg caactgaccc gcgacacggt gctatgcacc cagccgccca gccgtctgcc
     2821 aacctcgctg gcggtggtgc aggtcaccga tggacaaccc agctacggtt tctaccgcga
     2881 gggcattgcc gaccgcgact acaccgtctc ccaggtgctg gaggcgctgc acaaagtccc
     2941 gcccggtgtt ctgcatacgg gctcgctcat gctgataccc ccggagcacc acaaggtgct
     3001 ggagttgctg cagggtgcaa aagccatggg ctggaagatc agtgtggatg tgaacctgcg
     3061 gccccaactc gccgaagacc tggacgccta ccttgccgca gtccatgcgg tggcggcact
     3121 ggccgactgg atcaaggcca gcgacgaaga tctggagttg ctgggcttcc cggtggccac
     3181 acgcgcacag gccagcgaga ttgcacgctt ttttcaggac cagggtgcaa cccgtgttgc
     3241 gttgacattt ggtgcgcagg gcgcctggct ggcagtagac ggcgttacgg ccgaggcaga
     3301 tgctcccggg attacgctgg tcgactccgt cggcgccgga gacaccttct ggggcaactg
     3361 tctggccgaa tggacgctgg agctggacca cgccccgcaa cgcgttgcgc gcaccctgca
     3421 gctggccatg gcggcggctg ccatcaactg catgcgtgca ggctgccagc cgcccaccag
     3481 tgccgagctg ttggcggcaa ccactttggc ctgaagctgc gccccggatt caggcgacga
     3541 acacccgggc cttgcgtggt gtgagcacca gcacttcgcc ttctttgtag gcctgttcgc
     3601 gaaactgctg ggcgccaatc tgggcttcaa tgatgtcgcc gcttccggcc gctgaagcat
     3661 ccaggggcag cagctccagc cgcgcaatcg gtcccaccac aatggcgcgc accagcttgg
     3721 ccggaatgcc gcgttgctgc tggtagccgt tcagatggtt gtcttcaccg gcctcatagc
     3781 gccgcacgtc caggtcgtgt ggacgcacat aggcaaacgc cttggcgtcc tgcgcgccgc
     3841 tgtgctcggg gctggacagg cgcacgccgt ggtggtcttc gccaatccgc atttcgccct
     3901 cgtgggcgcg cccgttgaac agattcacgt cccccagaaa accatacaca aaggggctgg
     3961 cgggatggtc ccacacctct tggggcgagc caatctgctc cacctggccg ctgttcatca
     4021 gcaccacgcg gtcggccact tccagggcct cctcctggtc gtgggtcaca aagatgctgg
     4081 tcacatgcag gtcgtcgtgc aggcggcgca gccagcgacg cagttccttg cgcaccttgg
     4141 cgtccagcgc gccaaagggt tcgtccagca gcagcacctt gggctccacg gccaaggcac
     4201 gggccagcgc aatgcgctga cgctggccgc ccgacagttg cgcggggtag cggtcggcca
     4261 gccagtccag ctgcaccagg cccagcaggt cgtgcacttt ctgcttgatg acgatttcag
     4321 acgggcgcgt ggcgcgcggc ttcatgcgca ggccaaaggc cacgttgtca aacaccgtca
     4381 tgtggcggaa cagcgcatag tgctggaaca caaaacccac gttgcgctcg cgcacatgca
     4441 cgtcggtggt gtcttcaccg ctgaacagga tgttgccttg gtcggcggtt tccagcccgg
     4501 caatgatgcg cagcagggtg gtcttgccgc agcctgaggg gccgagcaag gcgacgagtt
     4561 ctccggactc gatgtccaga ctgacatcgt tcagcgcctt gaaggtgcca aattctttgc
     4621 tgacgttgcg aatggcaatg ctcatggtgt gtctcgaaaa gattcagggt aggaccgggg
     4681 ccaggggccg ctcgggcggc agtgctgcag cggccttctg gtcacgctca aactgccatt
     4741 ccacaaaaga cttgatgacc agggtgacta gcgccagcag cgccagcagc gaggcaatgg
     4801 caaacgcggc gacggactgg tactcgttgt acaaaatttc cacatgcagc ggcatggtgt
     4861 tggtctggcc acgaatgtgc ccggacacca ccgacaccgc gccaaattcg cccatggcac
     4921 gtgcgttaca caagatgacg ccgtagatca ggccccactt gatgttgggc agcgtcacat
     4981 gccagaaggt ctgccagcca ctggcgccca gcacttgggc ggcttgttct tcgtcggtgc
     5041 cctgcgcctg catcaggggg atcaactcgc gtgcgatgaa ggggaaggtc acaaagatgg
     5101 tggccagcac gatgccgggc acggcaaaca cgatcttgat gtcatgcgcc tgcagccacg
     5161 ggccaaacca gccgtgcgcg ccaaacatca gtacatagat cagcccagcc accacgggcg
     5221 acaccgagaa gggcaggtcc accagcgtgg tgaggaaggc cttgccacgg aactcgtact
     5281 tggcaatcgc ccaggcggcg gccacgccaa acaccaggtt cagcggaaca gcaatggccg
     5341 cggtgatgag cgtgaggcgg atggcgctcc aggcgtcggg ttccttgaag gcttcgaaga
     5401 acgcatcggc acctttgcgc agcgcttcgg taaacaccgc cgccagtggc agcaccagga
     5461 ataaaaagat aaacgtcagc gcaaggccta tcagcgtcca cttgacccat gcgggttcgg
     5521 tggtgccggc tttggcgcgg cggacatccc gtgcccccac gcttgtcgct gcgcgtactg
     5581 cgctgctccc cgagggggcg ctcacacctt gggacggccc ggcggtgttc ggtcgggagt
     5641 tactcatggt caggctcccg agcgtttacg ttgccatgac tgcaggccgt tgatgaccag
     5701 cagcatgaag aaggaggcca gcagcatcac ggtggccacg gcggtggcac cggcgtagtc
     5761 gtactgctcc agcttgccga tgatgaggag cggcgtgatc tccgacagca tgggcatgtt
     5821 gccggcaatg aagatgacgg agccgtactc cccgacaccg cgagcaaatg ccatggcaaa
     5881 accggtgagc agggcggggc caatggtggg gaaaatcacg taacggaagg tctgccagcg
     5941 tgtggcaccc aggcaggtgg cggcttcttc cagctctttc tcggcgtctt ccagcaccgg
     6001 ctgcacggtg cgcaccacaa agggcaggcc gatgaagatg agggcgatca ccacgccatt
     6061 gcggttgaag gcgagcgtga tgccataggg ctccagaatg ctaccgaccc agccattgct
     6121 ggcgagcagc gccgtgagcg agataccggc cacggcggtg ggcagcgcaa acggcaggtc
     6181 caccagcgcg tccacaattt ttttgccggg gaactggtag cgcaccagca cccaggccac
     6241 cagcaggcca aacaccgcgt tgaccagcgc ggccagaaac gaggcaccaa aggtgagctg
     6301 gtaggacgcg atgacgcgcg ggctgctcac ggcgctgacg aactgctccc agctcagcgt
     6361 gaaggttttg aacaccaatg ctgagagcgg gatcagcacg atcagcgcca ggtaaaacag
     6421 cgtgaacccg agcgtgaggc gaaagcccgg tagcacccgc ttgggtgcgc gctttgcaac
     6481 cgtcatttac ttgacggtgt agagcttgtc gaactggcca ccgtcgttga agtgcacttt
     6541 ttgcgcttca gcgaacgaac caaagtagtc ttcagcgcgg aacagggcaa tcggcttgaa
     6601 ggtgctggcg tacttcttca ggatcgccgg gttggtcgga cgaatggcat gcttggcggc
     6661 aatctcctgg ccttcgtcgc tgtacagaaa gttcaggtag gccttggctt gttcaaccgt
     6721 accttttttg gccacggtgc gttccaccac ggccacgggg ttctctgcca cgatggaact
     6781 gctggggtgg atagcgtcca ccttgccggc gccaaactcc ttgtccaccg acaccacttc
     6841 ggattcaaac gtgatcagca catcgccaat attgcgttgc aggaagatgc tggtcgcgtc
     6901 ccggccaccc ttggccagca cgggcacgtt tttgtagagc ttgctcacaa actcggcggc
     6961 ttg
//
LOCUS       DAOSJM010000084         6936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9908_length_6936_cov_3.549339, whole genome shotgun sequence.
ACCESSION   DAOSJM010000084 DAOSJM010000000
VERSION     DAOSJM010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9908_length_6936_cov_3.549339"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1936
                     /locus_tag="PKX82_04360"
     CDS             89..1936
                     /locus_tag="PKX82_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOF51514.1"
                     /translation="MSWAGKILRVDLTAGTVKSEPLNMQWARAYIGSRGLGTKYFTEE
                     VDAKVDPLSAANKIIWATGPLTGTMASTGGRYTVITKGPLTGAIACSNSGGYWGAEFK
                     MAGWDMVIFEGKSAKPVYLYVNDDVAELRDASHLWGKSVWETEETLKKSLQDPLTRVS
                     SIGKAGENGVLFAAVVNDLHRAAGRSGVGTVMGSKNLKAIAVRGTKGVGNIRDPKAFM
                     EAVKVSKKVLADNAVTGQGLPTFGTQVLMNVINEVGALPTRNHRDVQFESAKDISAEA
                     MATPRATDGKKQLVTNQACFGCTIACGRISKMDQGHFTVATKPQYWGASGGLEYEAAW
                     ALGAANGVKDLEALQYATMICNEEGIDPITFGATIGAVMELYEMGVLTREQLGIDAKF
                     GSAEALVFFVEETVNGRGFGKEIGQGSKRLTAKYGHPDLSMSSKGQEFPAYDGRAIQG
                     IGLAYATSNRGGCHLRGYTIASEVLGIPVKTDPLEHEGKPDLVKAFQDATAAFDSSGL
                     CIFTTFAWGLQDLAPQMQGACDEAYTIEELALIGERIWNMEREFNNRAGFTAKDDSLP
                     KRLLTEACKTGPAKGKVNMLAEMMPKYYAARGWDAEGRPTAETRKRLSL"
     gene            1944..3239
                     /locus_tag="PKX82_04365"
     CDS             1944..3239
                     /locus_tag="PKX82_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51515.1"
                     /translation="MTHHLILGAGPAGVIAAETIRKLSPTDAITLVGDEPEPPYSRMA
                     IPYLLMGNVDERGTYLRKGASHFADLKVNVVRARVTSVQIATKTVALDNGQSLAFDTL
                     LVATGSHPIRPPIPGIGSAGVHACWTLDDARKIMALATRGARVLQLGAGFIGCIIMEA
                     LAARGVKLSVVEMGDRMVPRMMGPTAGGMIRDWCEAKGVQVFTGTKVESITPGTPLQV
                     KLSNGQTMEADLVISAAGVRPAIGYLENSGIQCLLGVLTDEHLQTNVPGVYAAGDCAE
                     AFDKVSGKTIVSAIQPNAAEQARVAAANMVAFAQGKAPAANLKGVTQINVLDTLGLIS
                     TSFGDWQGRPGGEHVELTDLPAGRHLSLQFKDDVLVGCNSVGWTEHVGVMRGLVEGQI
                     KLGAWKDTLKNDPTKLVEAYLACAQGQEAWSGANDARRR"
     gene            3252..3506
                     /locus_tag="PKX82_04370"
     CDS             3252..3506
                     /locus_tag="PKX82_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOF51516.1"
                     /translation="MKITFKLFASLTEYLPQQARYTNVVELDVAPDASILQIIEPFGL
                     PPKLVHLVLINGRYVEPEKRMTQTLVEGDVLAIWPPIAGG"
     gene            3506..3805
                     /locus_tag="PKX82_04375"
     CDS             3506..3805
                     /locus_tag="PKX82_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51517.1"
                     /translation="MQSFYEERFERDMGCTEADWLRWLPDAIGDHHWKIQGQTAGVRI
                     GDGALGLKWQVAEPRVIALVRMPRLLVHFRFAGVDEAARYTFMKRFDLYMQRGGG"
     gene            3845..4360
                     /locus_tag="PKX82_04380"
     CDS             3845..4360
                     /locus_tag="PKX82_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HOF51518.1"
                     /translation="MKKVWLPLSLVALLSACAAYAPGPVATAQLAATKGNAVTGSVRF
                     EQMGNKVRVSGEVQGLAPNRVHGFHVHEKGDCSSGDGMGTGGHFNPGSTAHGAHDADA
                     HHAGDLASLKADANGVARFSYDAQGISVDAGAASVVGRGLIVHRDPDDYKTQPTGNSG
                     PRVACAVIQKI"
     gene            4454..4675
                     /locus_tag="PKX82_04385"
     CDS             4454..4675
                     /locus_tag="PKX82_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51519.1"
                     /translation="MNPTKLLSVILIVGGVLGLLYGSFSYTKDTTALKIGPLELAVKE
                     KETVNIPMWAGVGAIVAGGLLLVLGSKKS"
     gene            complement(4672..5799)
                     /locus_tag="PKX82_04390"
     CDS             complement(4672..5799)
                     /locus_tag="PKX82_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOF51520.1"
                     /translation="MHRLQFHYTLSRDSSPALVRNPLIDLLQAVAAHGSISAAARALD
                     LSYRHVWGELKRWEQELGNELLVWEKGQAARLSEFGAKLLWAERQAQARLAPQIEALR
                     GDLERAFAVAFDPHAHVVTLYASHDDALAVLREYALADHTAQSNAEQLHLDIRFCGSV
                     DAIRALNEGRCVMAGFHTLQHPQPGSLAEQVYKPLLQPGLHKVIGFAERTQGLMVAPG
                     NPLGLLSLADVQRTGARFVNRALGTGTRVVVDELLAQAGIAGKDLRGYDTTEPSHTAA
                     AHAVASGSADVALGIAIAAQAQGLDFVPLVQESYHLVCLKSALQEPAIAALLEVLQSP
                     AWQDRLAGIAGYTPSHCGEVLSLRKVLGWWSFARPKTRKPL"
     gene            5850..6590
                     /locus_tag="PKX82_04395"
     CDS             5850..6590
                     /locus_tag="PKX82_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51521.1"
                     /translation="MTSLTESATTALQLVASLDPVLLSIVWRSLSVSGVACLLACGAG
                     LVLGAWLGVARFPGRGAVLTLLNTFLAVPSVVVGLVVYLLLSRSGPLGFLGWLFSFQA
                     MVLAQALLVLPVVTALTRQVVEDAESLHGEQLASLGARPLMRSLLLAWDERFALLTVV
                     IASFGRAISEVGAVMLVGGNIDGFTRVMTTAIALETSKGDLPLALGLGLVLLGVVLVL
                     NAITGLLRRWRESQGGVGVAMPQMGVSG"
     gene            6587..>6936
                     /locus_tag="PKX82_04400"
     CDS             6587..>6936
                     /locus_tag="PKX82_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF51522.1"
                     /translation="MTHQLEIANAGVRYGRSAAAVQALRGVSLRIQAGERVALVGANG
                     CGKSTLLRLMHGLIAPSEGTVRHQVGVQQAMLFQRPYLMRLSVLNNVALGLWLRGTPW
                     AQARDKALHALQRVG"
BASE COUNT         1256 a         2074 c         2308 g         1298 t
ORIGIN      
        1 tcgacgccaa ctggaccggc ctgggcaaga tgcaggcgtg ggcagacaaa ttgggcaacc
       61 agccctcggc aacttaagga gcagcaccat gtcatgggca ggaaaaattc ttcgtgtgga
      121 tctgaccgcc ggtactgtca agtccgagcc actcaatatg caatgggcgc gtgcctacat
      181 cggttcgcgc ggactgggta ccaaatactt tacggaagag gtggacgcca aggtcgatcc
      241 cctctccgcc gccaacaaga tcatctgggc cactggcccg ctgaccggca ccatggcctc
      301 cacgggtggc cgttacaccg tcatcaccaa ggggccgctg acgggcgcta ttgcctgttc
      361 caactcgggt ggctactggg gcgcggagtt caagatggcc ggctgggaca tggttatttt
      421 tgaaggcaag tcggccaagc cggtctacct gtacgtcaac gacgacgtgg ccgaactgcg
      481 tgatgcctcc cacctgtggg gcaagagcgt gtgggaaacc gaagagacgc tcaagaagag
      541 cctgcaggat ccgctgacgc gcgtgtcgag catcggcaag gccggtgaaa atggtgtgct
      601 gtttgcggcc gtggtcaatg acctgcaccg cgcggcaggc cgctcgggtg tgggtaccgt
      661 gatgggtagc aagaacctca aggccattgc ggtgcgcggc accaaggggg tgggcaatat
      721 ccgcgatccc aaggccttta tggaagcggt caaggtcagc aaaaaggtgt tggcggacaa
      781 cgctgtgaca gggcagggat tgcccacctt tggcacccag gtgctgatga acgtgatcaa
      841 tgaagtgggc gcgctgccca cacgcaatca ccgcgatgtg cagttcgaga gcgccaagga
      901 catctccgcc gaagccatgg caacgccgcg cgccaccgat ggcaagaagc agctggtgac
      961 caatcaggcc tgctttggct gcaccattgc ctgcggacgc atcagcaaga tggaccaggg
     1021 ccacttcacc gtggccacca agccgcagta ctggggcgcc tcgggcggtc tggagtacga
     1081 agccgcctgg gcactgggcg ctgccaatgg cgtgaaagat ctggaggcac tgcaatacgc
     1141 cacgatgatc tgcaacgaag agggtatcga tcccattact tttggtgcca ccattggcgc
     1201 ggtgatggag ctctacgaga tgggtgtgct gaccagggag caactgggta tcgatgccaa
     1261 gtttggttcg gccgaagcgc tggtgttctt tgtggaagaa accgtgaacg gccgtggctt
     1321 tggcaaggaa attggccagg gctccaaacg cctgaccgcc aagtacggcc accccgacct
     1381 gtccatgagc agcaagggcc aggagttccc ggcgtatgac ggccgcgcca tccagggcat
     1441 tggcctggcc tatgccacct ccaaccgtgg tggctgccat ctgcgcggtt acaccatcgc
     1501 gtcggaagtg ctgggtatcc cggtcaagac cgatccgctg gagcacgaag gcaaacctga
     1561 cctggtgaaa gccttccagg acgcgaccgc cgctttcgac tcgtcgggcc tgtgcatctt
     1621 caccaccttt gcctggggcc tgcaagacct ggcgccgcag atgcagggcg cgtgtgatga
     1681 ggcctacacc atcgaagagc tggccctgat tggcgagcgc atctggaaca tggagcgcga
     1741 gttcaacaac cgtgccggct ttaccgccaa ggacgacagc ctgcccaagc gcctgctcac
     1801 cgaagcctgc aagaccggcc ctgccaaagg caaggtcaac atgctggccg agatgatgcc
     1861 caagtactac gctgcgcgcg gatgggatgc ggaaggccga cccactgccg aaacgcggaa
     1921 acgcctgagc ctctgatacc tcgatgacgc accacctcat tcttggcgca ggtcccgcag
     1981 gcgtgatcgc cgcggagacc atccgcaaac tgagcccgac cgacgccatc accctcgttg
     2041 gcgacgagcc ggagccgccg tattcgcgca tggccattcc ctacttgctg atgggcaacg
     2101 tggatgagcg cggcacctat ctgcgcaagg gcgccagcca ttttgcggat ttgaaggtga
     2161 atgtggtccg ggcccgcgtc acgtctgtgc agatagctac taaaactgta gcgctcgaca
     2221 acgggcagag cctggcgttt gacacattgc tggtcgccac cgggtcgcat cccatccgtc
     2281 cacccatccc gggcattggg tcggccggtg tgcatgcctg ctggacgctg gacgatgccc
     2341 gcaagatcat ggcgctggcc acccggggtg cgcgcgtctt gcagctgggt gccggcttta
     2401 tcggctgcat catcatggaa gcactggccg cgcgcggcgt gaagctctct gtcgttgaga
     2461 tgggggaccg catggtgccg cgcatgatgg gacccaccgc cggcggcatg atccgcgact
     2521 ggtgcgaggc caaaggcgtg caggtgttta ccggcaccaa ggtggagtca atcacaccgg
     2581 gcacgccctt gcaggtcaag ctctccaatg gccagacgat ggaagccgat ctggtcatca
     2641 gtgccgccgg tgtgcgcccg gccattggct atctggagaa ctcgggcatc cagtgcctgc
     2701 tgggcgtgct gaccgatgag catctgcaga ccaatgtgcc cggcgtgtac gcggcgggtg
     2761 actgtgcgga ggcttttgac aaggtctcgg gcaagaccat cgtcagcgcc atccagccca
     2821 atgccgcgga gcaggcccgt gtggctgccg ccaatatggt ggcctttgcc caaggcaagg
     2881 cgccagcggc caacctaaag ggtgtgaccc agatcaatgt gctcgacacc ctgggcctga
     2941 tttccaccag cttcggcgac tggcagggcc gccccggtgg cgagcacgta gagttgaccg
     3001 acctgcctgc gggccgtcac ctgagcctgc agttcaagga cgatgtgctg gtcggttgca
     3061 acagtgtggg ctggaccgag cacgtgggtg tgatgcgtgg gctggtcgaa gggcagatca
     3121 agctgggtgc atggaaagac accctgaaaa atgaccctac caaactcgtg gaggcctacc
     3181 ttgcttgtgc gcagggtcaa gaagcctggt ccggcgccaa cgatgcgcgg cgcaggtagt
     3241 attccgacag catgaagatc actttcaagt tgtttgcatc gttgacagag tacctgccgc
     3301 agcaggcgcg ctacaccaat gtggtggagc tggatgtggc gccggacgct tccatcctgc
     3361 agatcattga gccgtttggc ctgccgccca agctggtgca tctggtgctg atcaacggac
     3421 gttacgtgga gccggagaag cgcatgaccc agacattggt cgaaggcgat gtgctggcga
     3481 tctggccacc gattgcggga ggttgatgca atcgttttac gaagagcgct ttgagcgcga
     3541 catgggctgt accgaggcgg actggctgcg ctggttaccc gatgcgattg gtgaccatca
     3601 ctggaagata caggggcaaa ctgccggtgt gcgcattggc gatggcgcac tcggcctcaa
     3661 gtggcaggtg gccgaacccc gcgttatcgc gctggtgcgc atgcctcgcc tgctggtgca
     3721 tttccggttt gcgggagtgg atgaagcggc ccgttacacc ttcatgaagc gctttgacct
     3781 gtacatgcag cgcggaggag ggtaaaaggg cggttgggag tttttcaatg agaggaaatt
     3841 cgcaatgaaa aaagtatggc ttcccctgtc tctggttgca ctgttgtctg cgtgcgctgc
     3901 ctatgccccg ggcccggtgg caaccgccca gctggcggct accaagggca atgcggtcac
     3961 aggctccgtc cggtttgaac agatgggcaa caaggtgcgt gtgtcgggcg aggtgcaggg
     4021 actggcaccc aaccgcgtcc atggtttcca tgtgcacgaa aagggcgact gctccagcgg
     4081 tgacggcatg ggaacgggtg gacacttcaa tcccggcagc acggcgcatg gcgcgcacga
     4141 tgccgatgcg caccatgctg gcgacctggc cagcctgaag gcagatgcca atggcgttgc
     4201 ccggttcagc tatgacgcgc agggtatttc tgtggacgcg ggtgcggcca gtgtggttgg
     4261 gcgcggtctg atcgtccacc gtgatcccga cgactacaag acgcagccca ccggcaactc
     4321 cggtccgcgt gtggcttgcg ccgtcattca gaaaatctga caccttggcc tgaccgggtc
     4381 tggggacccg ttcgtgcgac agcgcacaga cgcctttgcg ttgcaggatc aaactgcaag
     4441 cgaaggaaac cctatgaatc ccactaaatt gttgtccgtc atcctgatcg ttggcggtgt
     4501 gctcggcctg ctgtacggca gcttcagcta caccaaagac accaccgccc tgaaaattgg
     4561 cccgctggag ctggcggtga aggaaaaaga aacggtgaat atccccatgt gggccggcgt
     4621 aggtgcgatc gtcgccggcg ggctgctgct ggtgctgggc agcaagaaga gctagagagg
     4681 ttttcgggtt ttggggcgtg caaagctcca ccagcccaac accttgcgca gcgacagcac
     4741 ttcgccacag tgcgatggcg tgtagcccgc aatgccggcc agccggtcct gccaagcggg
     4801 gctctgcaga acttcgagca gtgcagcaat ggctggctcc tgcagggctg atttcaggca
     4861 caccaggtgg tagctttctt gtaccagcgg cacaaaatcc agcccctgcg cttgcgcagc
     4921 gatggcaatt cccaatgcca catccgccga tccggacgct actgcatggg ctgccgcggt
     4981 gtgggatggt tcggtggtgt cgtagccacg caggtctttg cccgcgatgc cggcctgtgc
     5041 cagcagttcg tccacgacca cccgtgtgcc ggttcccagt gcccggttga caaaacgcgc
     5101 gcccgtgcgt tgcacatccg ccagtgacag cagccccagt ggattgccag gcgccaccat
     5161 cagcccctgg gtacgctcgg caaagccgat gaccttgtgc aggcccggct gcagcagcgg
     5221 cttgtagacc tgctcggcca gtgagccggg ctgagggtgc tgcagggtgt gaaagccggc
     5281 catcacgcag cggccttcgt taagggcgcg gatggcatcc acgctgccac aaaaacggat
     5341 atccaggtgc agctgctccg cattgctttg cgctgtgtgg tcggccagcg cgtactcgcg
     5401 caacaccgcc agcgcatcgt cgtggctggc atacagcgtg acgacgtggg catgcggatc
     5461 aaaagccacc gcaaatgcgc gctccagatc cccccgcagg gcctcgattt ggggtgccag
     5521 acgggcctgc gcctggcgct cggcccagag cagcttggcg ccgaactccg agaggcgtgc
     5581 ggcctgcccc ttttcccaga ccaacagttc gttgcccagc tcctgctccc agcgcttgag
     5641 ctctccccac acatggcggt acgacaggtc cagtgcgcgc gctgccgccg aaatggagcc
     5701 gtgggccgcc acggcctgca gcaggtcgat gagcgggttg cggaccagcg ccgggctgct
     5761 gtcgcgggag agggtgtagt ggaactggag cctatgcacg gcgtttcata tcgctggtaa
     5821 ttgggtcggt ggctacgata ccccaagtta tgacatcact taccgaaagc gcgaccacag
     5881 cgctgcaact cgttgcctcc ctcgatcccg tgctgctcag tattgtctgg cgctcgctct
     5941 ccgtcagcgg cgttgcctgt ctgctcgcgt gtggcgctgg gctggtgctg ggggcctggc
     6001 tgggcgtagc ccgctttccc ggccgcgggg ccgtgctcac gctgctcaat acctttctgg
     6061 ccgtgccttc ggtggtggtc gggctggtgg tgtacctgct gctgtcgcgc tccggcccgc
     6121 tgggttttct ggggtggctc ttcagctttc aggccatggt gctggcgcaa gcgctgctgg
     6181 ttctgccagt cgtcacggcg ctgacgcgcc aggtggtgga agacgctgag agcctccacg
     6241 gcgaacagct ggcttctctg ggcgcgcgtc ccctcatgcg cagcctgctg ctggcgtggg
     6301 acgaacgctt tgcgctgctg accgtggtca tcgcgtcttt tggccgtgcc atctccgagg
     6361 tgggtgcggt catgctggtg ggtggcaaca tcgacggctt tacccgcgtg atgactactg
     6421 cgattgcgct ggaaaccagc aagggtgatc tgccgctggc cctgggcctg ggcctggtgc
     6481 tgctgggcgt ggtgctcgtg ctgaacgcca tcaccggctt gttgcggcgc tggcgggagt
     6541 cccagggcgg cgtgggtgtg gccatgccgc agatgggagt cagcggatga cgcaccagct
     6601 ggaaatcgct aacgccggcg tgcgctacgg ccgctccgcg gctgcggtgc aggcactgcg
     6661 gggtgtcagc ctgcgcatac aggctggcga gcgcgtggcc ctggtgggtg ctaacggctg
     6721 cggcaagagt actttgctgc ggctgatgca tgggctgatc gcgcctagcg agggcacggt
     6781 gcggcaccag gtgggtgtgc agcaggccat gctgttccag cggccctatc tgatgcgcct
     6841 gtcggtcctg aacaatgtgg cgttgggctt gtggctgcgt ggcacgccgt gggcccaggc
     6901 gcgtgacaag gccttgcacg cgttgcaacg ggtcgg
//
LOCUS       DAOSJM010000085         6930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_9920_length_6930_cov_5.400000, whole genome shotgun sequence.
ACCESSION   DAOSJM010000085 DAOSJM010000000
VERSION     DAOSJM010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9920_length_6930_cov_5.400000"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKX82_04405"
     CDS             complement(<1..595)
                     /locus_tag="PKX82_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF51523.1"
                     /translation="MARENPLHQRRRPLTEAELEAIPWFPLLAPDERKRAEEDLKIAQ
                     AAPGDFVCRMGRPVTYWFGVVDGLLKMSSDNAQGQTMTFTGVPPGGWFGEGTALKREV
                     YRYNIQALRKSMVAGLHVDTFHWLLDHSIAFNRFIMNQLNERLGQFIAAREIDRMTNP
                     DIRVARSLATLFNPVLYPGVGEVLRITQQELAYLVGLS"
     gene            800..2743
                     /locus_tag="PKX82_04410"
     CDS             800..2743
                     /locus_tag="PKX82_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF51524.1"
                     /translation="MQTTFPQLLLQHASSRPTAAAMREKEYGIWQSLTWADLATMVEH
                     LAAGLHQAGLRRGEHMVVVGANRPRLYATMLAVQSLGAIPIPLYQDAVAAECVFPINN
                     AEVRFAFAEDQEQVDKLLEIRESCPQLQHVFFDDPRGLRNYDEPGLAAMDELQAAGKA
                     FAAIHTDFFKEEVARIVPTDVAAMFFTSGTTGNPKGVVHTHDSLLNRARAGADFDKLT
                     SSEEVLAYLPPAWIGQNIFSYAQWLSCGYVVNCPESSATATIDLKEIGPTYYFAPPRV
                     FEGLLTSVMIRMEDAGTLKRGMFHFFMAVAKRCGPALMDGKEVSFTDRLLYALGNLMV
                     YGPLRNNLGMSRVRVAYTAGEAIGPDLFTFYRSIGVNIKQLYGSTETAVFVCLQPDNE
                     ARADTVGVPCEGVEIKVADNGEILVKSPGLLKYYKNPAATAEVLTAEGWYHTSDAGFI
                     DAHGHLKIIDRVKDVGRIKGGANDGAMFAPKYVENKLKFFQHIKEVVAYGDGRDRVCV
                     MINIDFDAVGNWAERRNLPYAGYTDLAQKTQVYQLIKECVEKVNADLAEDTLLAGSQV
                     SRFLVLHKELDADDGELTRTNKVRRGFIAEKYQPLVDALYSDLTSQYIETVVKFEDGR
                     TGKVSATLRIDDAKTFAPAKAAA"
     gene            2758..3543
                     /locus_tag="PKX82_04415"
     CDS             2758..3543
                     /locus_tag="PKX82_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF51525.1"
                     /translation="MSKKKIGDVILDVKNISLGFGGVKALTDISFNVKEHEIRAIIGP
                     NGAGKSSMLNCINGVYTPQQGSITFRGQEFKHMDSHQVATMGIGRTFQNLALFKGMSV
                     LDNIMSGRNLKIKSNILMQALRIGPAEREEIMHREAVERIIDFLEIQAYRKTPVGQLP
                     YGLQKRVDLGRALAMEPQVLLLDEPMAGMNVEEKQDMCRFVLDVNDEFGTTVVLIEHD
                     MGVVMDISDRVVVLDYGKKIGDGTPDEVRNNEDVISAYLGTSH"
     gene            3555..4484
                     /locus_tag="PKX82_04420"
     CDS             3555..4484
                     /locus_tag="PKX82_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF51526.1"
                     /translation="MAFFLETLLGGLMAGMLYSLVALGFVLIFKASGVFNFAQGAMVL
                     FAALAMARFAEWVPLYLGIEDPIIGNVLAFIGAGGVMFCVAWVIERLVLRHLVNQEGT
                     TLLMATLGITYFMEGLGQTIFGSSIYKIDIGMPKDPVMILESTFEGGIMINKEDFYAA
                     IIAAALVALLSVFFQKTATGRALRAVADDHQAAQSIGIPLNRIWVIVWCVAGVVALVA
                     GMIWGSKLGVQFSLTTVALRALPVVILGGLTSVPGAIVGGLIIGVGEKLSEVYLGPYV
                     GGGIEIWFAYVLALGFLLIRPQGLFGEKIIDRV"
     gene            4494..5570
                     /locus_tag="PKX82_04425"
     CDS             4494..5570
                     /locus_tag="PKX82_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF51527.1"
                     /translation="MFYRENGQFKTSYRADQQIFPITQDRIAILALLVVAFVVVPSLA
                     SDYMFRAIFIPFLIFSLAAVGVNILVGYCGQISLGSGAFMAVGAYAAYNFFVRIEGMP
                     LVVALILGGVSSMFFGIVFGLPSLRVKGLYLAVATLAAQFFADWMFLRIQWFTNYSPS
                     GSVSVSNLQVFGFSLESPFAKYMFCLVLLAVIALLAKNLVRGAVGREWMAIRDMDVAA
                     AVIGIRPMFAKLSAFAVSSFIVGVAGALWAFIYLGAWEPAAFSVDQSFKLLFMVIIGG
                     MGSIMGSFFGAAFIVVLPIFLNQFLPALASVLGIEISTAGVSHAELMIFGGLIVWFLI
                     VEPHGLAKLWSIAKQKLRLWPFPH"
     gene            5632..>6930
                     /locus_tag="PKX82_04430"
     CDS             5632..>6930
                     /locus_tag="PKX82_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF51528.1"
                     /translation="MKLRKTVLAVALVAAGASVLVTGNAFAQAKEQFIPVLSYRTGPY
                     APNGVPWANGYVDYIKLTNARGGINGVKFSFEECETGYDTARSVECYERLKSKGASFV
                     QPLSTGATFAITEKAPADKIPVVTVGYGRSESADGSVFKWNFPIAGTYWVAADAIMQA
                     IAKKEGGFDKLKGKKISLVYHDSPFGKEPIPLLQERAAMHGFTLTLLPVTAPGVEQKA
                     TWLQVRQNRPDYVVLWGWGVMNSTAIKEAQAAGYAREKMYGVWWAGAEPDVKDVAEGA
                     KGYNAVTMQHGYEPNSKVVKDIQAMVHAKGQGTGPKEELGQVLYMRGAMSAMLGIEGV
                     RAAQERFGKGKVMTGEQIRWGLENLNLTQPKLDALGFAGVMRPVSTSCTDHMGANWAR
                     IHTWDGKGWKFTSDWLQADEQILKPLVKTVAAKYAAEKKLA"
BASE COUNT         1349 a         1961 c         2194 g         1426 t
ORIGIN      
        1 gcgacagccc caccaggtag gccagctcct gctgggtgat gcgcagcacc tcgcccacgc
       61 cggggtagag caccgggttg aacagcgtgg ccaggctgcg cgccacgcgg atgtcggggt
      121 tggtcatgcg gtcgatttcg cgcgcggcga tgaactgccc caggcgctcg ttgagctggt
      181 tcatgatgaa gcggttgaag gcgatggagt ggtccagcag ccagtggaag gtgtccacat
      241 gcaggccggc caccatactt ttgcgcagcg cctggatgtt gtagcggtac acctcgcgtt
      301 tgagggcggt gccttcgcca aaccagccac cgggcggaac gccggtgaag gtcatggttt
      361 gcccctgggc gttgtccgag ctcatcttga gtaggccgtc caccacgcca aaccagtagg
      421 tgacgggccg acccatgcgg cagacaaagt cgcctggggc ggcctgggca atcttcaaat
      481 cttcttcggc gcgtttgcgc tcatcgggtg cgagcaaggg gaaccagggg atggcttcca
      541 gctcggcctc ggtcagggga cggcggcgct ggtgtagcgg gttttcacgt gccatgaggg
      601 gtatgcgctc cgggtttgta agtctggatt cagtgaaggg aaaacaccta ggaagaattg
      661 ccttgattgt cgtccaaacg acagcttgac gtcaaaccat cgtcccataa tcgctccaca
      721 ttacagcccg tgttccgttg tgcggagtgc ggcgaggtaa ccggtttcag cgacaagaga
      781 catgcaaaga ggcatccgaa tgcaaacgac ttttccgcaa ctactactgc agcacgccag
      841 cagtcgcccc acggctgcgg ccatgcgcga gaaggaatac gggatctggc aatcactgac
      901 ctgggccgat ctggccacca tggtcgagca cctggccgca ggcctgcacc aggccggtct
      961 gcgccgcggt gagcatatgg tggtggtggg cgccaaccgc ccccggctgt atgccaccat
     1021 gctggcggtg cagtcgctgg gcgcgattcc gattccgttg taccaggacg ctgtggcggc
     1081 cgagtgcgtg ttccccatca acaatgcgga agtgcgtttc gcctttgccg aagaccagga
     1141 gcaggtcgac aagttgctgg agattcgcga aagctgcccg cagctgcagc acgtcttttt
     1201 cgatgacccg cgtggtctgc gcaattacga cgagcctgga ctggctgcca tggatgagtt
     1261 gcaggctgcc ggcaaggcct tcgcagccat ccacaccgac ttcttcaagg aagaagtggc
     1321 gcggattgtg ccgacggatg tggcggcaat gtttttcacg tccggcacca cgggcaaccc
     1381 caagggtgtg gtgcatacgc acgattcgtt gttgaaccgt gcgcgcgccg gtgcggactt
     1441 tgacaagctc accagcagcg aagaggtgct ggcctacctg ccgccggcct ggattggcca
     1501 gaatatcttc agctatgcac agtggttgtc gtgcggctac gtggtgaact gccctgagag
     1561 cagcgccacg gccaccatcg acctgaagga aattggcccg acctattact ttgcaccgcc
     1621 ccgggtattt gaaggcctgc tcaccagcgt gatgattcgc atggaagatg cgggcacgct
     1681 caagcgcggc atgttccatt tcttcatggc tgtggccaag cgctgcggcc ccgcgctcat
     1741 ggatggcaag gaagtgtctt tcaccgaccg tctgctgtat gcgctgggca acctcatggt
     1801 gtacggcccg ctgcgcaaca acctgggcat gagtcgggtg cgtgtggcct ataccgccgg
     1861 cgaggccatt ggcccggacc tgtttacctt ctaccgctcc attggtgtca acatcaagca
     1921 gctgtacggc tcgaccgaaa cggccgtgtt cgtgtgcctg cagcccgata acgaggcccg
     1981 tgccgatacg gtgggtgtgc cctgcgaggg ggtggaaatc aaggtggccg acaacggcga
     2041 aattctggtc aagtcgcccg gactgctcaa gtactacaag aacccggcgg ccacggctga
     2101 agtgctgacg gccgaaggct ggtaccacac cagcgacgcc ggcttcatcg acgcgcatgg
     2161 ccacctgaag atcatcgacc gcgtgaaaga cgtgggtcgt atcaagggcg gtgccaacga
     2221 cggcgccatg tttgcgccca agtatgtgga gaacaagctc aagttcttcc agcacatcaa
     2281 ggaagtggtg gcttacggtg acggacgcga ccgcgtgtgc gtgatgatca acatcgactt
     2341 tgacgccgtg ggcaactggg ccgagcgtcg caacctgccg tatgccggat acaccgatct
     2401 ggcacaaaaa acacaggtct accagctcat caaggagtgc gtggaaaagg tcaatgccga
     2461 tctggccgaa gacacgctgc tggcaggcag ccaggtcagc cgattcctgg tattgcacaa
     2521 ggagcttgac gccgacgatg gcgagctcac ccggaccaac aaggtgcgcc gtggcttcat
     2581 cgccgaaaaa taccagccgc tggtggacgc cttgtacagc gatctgacaa gccagtacat
     2641 cgaaaccgtt gtcaagttcg aggatggccg caccggcaag gtcagcgcca ccctgcgtat
     2701 cgacgacgcc aagacattcg cgcccgccaa ggccgcagcc tgagatacgc acgcaccatg
     2761 agcaagaaaa aaattggcga cgtcatcctg gacgtcaaga acatttccct gggttttggt
     2821 ggcgtcaagg cgctgaccga tatctccttc aacgtcaagg agcacgagat tcgggccatc
     2881 attggcccca atggtgccgg caagagctcc atgctcaact gcatcaatgg tgtctacaca
     2941 ccgcagcagg gctccatcac cttccggggg caggagttca agcacatgga cagccaccag
     3001 gtggccacca tgggcattgg ccgcaccttc cagaacctgg cgctgttcaa gggtatgagc
     3061 gtgctggaca acatcatgtc cggccgcaac ctcaagatca agagcaatat cctgatgcag
     3121 gccctgcgca tcggcccggc cgagcgtgag gaaatcatgc accgcgaagc ggtggagcgc
     3181 atcatcgact ttctggaaat ccaggcctac cgcaaaacgc ctgtggggca gttgccctac
     3241 ggcctgcaaa agcgtgtgga cctgggtcgc gcactggcca tggagccgca ggtgctgctg
     3301 ctcgacgagc ccatggccgg tatgaatgtg gaagaaaagc aggacatgtg ccgctttgta
     3361 ctcgacgtga atgacgagtt cggaaccacc gtggtgctga tcgagcacga catgggggtg
     3421 gtgatggata tctccgaccg cgtggtggtg ctggactacg gcaagaagat tggcgatggc
     3481 acccccgacg aagtgcgcaa caacgaagac gtgatcagtg cctacctcgg cacgagccac
     3541 taaggaaaca cgacatggca ttctttctgg aaacactctt gggcggcctg atggccggca
     3601 tgctgtattc gctggtggca ctgggctttg tgctgatttt caaggcctct ggcgtgttca
     3661 actttgcgca gggcgcgatg gtgctgtttg ccgcgctggc aatggcccgt tttgccgaat
     3721 gggttccgtt gtacctgggc attgaagatc cgatcattgg caacgtgctg gcgttcattg
     3781 gtgcgggcgg cgtgatgttc tgcgtggcct gggtgatcga acgcctggtg ttgcgccacc
     3841 tggtcaacca ggaaggcacg accctgctga tggcgacgct gggcatcacc tatttcatgg
     3901 aaggcctggg ccagaccatc ttcggcagca gcatctacaa gatcgacatc ggcatgccca
     3961 aagacccggt gatgattctg gagagcacgt ttgaaggcgg catcatgatc aacaaggaag
     4021 acttctacgc cgccatcatt gcggcagcgc tggtggcctt gctgagcgtg tttttccaga
     4081 aaacggccac gggtcgtgcc cttcgcgcgg tggccgatga ccaccaggcc gcacaaagta
     4141 tcggtattcc gctcaaccgc atctgggtca tcgtgtggtg cgtggccggt gtcgtggcac
     4201 tggtggccgg gatgatctgg ggcagcaagc tcggagtgca gttttcgctg accaccgtgg
     4261 ctttgcgtgc gctgccagtg gtgattctgg gcggtttgac gtcggtgcct ggcgccattg
     4321 tgggcggcct gatcatcggt gtgggcgaga agctgtccga ggtgtatctg ggcccgtatg
     4381 tgggcggcgg tattgaaatc tggtttgcct atgtgctggc actgggcttc ctgctgatcc
     4441 gtccccaggg tttgtttggc gagaagatca tcgatcgcgt ttgagagaag actatgtttt
     4501 acagagaaaa cggccaattc aagacgagct accgggcaga ccagcagatc ttcccgatta
     4561 cccaggatcg catcgccatc ctggccctgc tggtggttgc ctttgtcgtg gtgccctcgc
     4621 tggccagcga ctacatgttc cgcgcgattt tcattccctt cctgatcttc tcgctggcgg
     4681 cagtgggtgt gaatattctg gtgggctatt gtggccagat ctcgctgggc tccggcgcct
     4741 tcatggcggt gggggcctat gcggcgtaca acttctttgt gcgcattgag ggcatgccct
     4801 tggtcgtggc gctgattctg ggtggtgtga gttccatgtt ctttggcatc gtgttcggtt
     4861 tgccgagtct gcgcgtgaag ggcctgtacc tggcggtggc gaccctggcg gcgcagttct
     4921 ttgccgactg gatgtttctg cgtatccagt ggtttaccaa ctactcgccc tcgggctcgg
     4981 tgtcggtgtc caacctgcag gtcttcgggt tttcgctgga gagccctttc gccaaataca
     5041 tgttctgtct ggtgctgctg gcggtgattg ccctgctggc aaagaacctg gtgcgcggcg
     5101 ctgtgggccg ggaatggatg gcgatccgtg acatggacgt ggcggctgcc gtgatcggca
     5161 ttcgccccat gttcgccaag ctcagcgcgt ttgcggtcag ctcgttcatt gtgggcgtgg
     5221 ccggtgcgct gtgggccttc atttacctgg gcgcctggga gccggcagcg ttctcggtag
     5281 accagtcgtt caagctgctg ttcatggtca tcatcggtgg catgggctcc atcatgggca
     5341 gcttttttgg tgccgcgttc attgtggtgc tgccgatttt cctgaaccag ttcctgccgg
     5401 cgctggccag cgtgctgggg attgaaatct cgacggccgg tgtatcgcat gccgagctga
     5461 tgatttttgg cggactgatc gtgtggttcc tgattgtgga gccccatggc ctggccaagc
     5521 tgtggtcgat tgccaagcag aagcttcgcc tttggccgtt cccccactga tcggattgac
     5581 ccgttttccc gtgttccctt gtgcttcaac attcattaac aggagagaca aatgaagctt
     5641 cgcaaaaccg tactcgcagt agcccttgtg gccgcaggcg catccgttct cgtaacgggc
     5701 aatgcatttg cacaggccaa ggagcagttc atccccgtgc tgtcctaccg cacaggcccc
     5761 tatgccccca atggcgtgcc atgggctaac ggctacgtcg actacatcaa gctgaccaac
     5821 gcacgcggcg gcatcaacgg cgtgaaattc tcgttcgaag aatgcgaaac cggctatgac
     5881 acggcccgta gcgtggaatg ctacgagcgt ctcaagagca agggcgcatc gttcgtgcaa
     5941 cccctgtcca cgggtgccac atttgccatt actgaaaaag cccctgcgga caaaattccc
     6001 gtggtgactg tgggctacgg ccgcagcgag agcgcggatg gttccgtgtt caagtggaac
     6061 ttccccattg ctggcaccta ctgggtggcc gcggatgcca tcatgcaagc gattgccaaa
     6121 aaagaaggcg gctttgacaa gctcaagggc aagaagattt ccctcgtcta ccacgacagc
     6181 ccgtttggca aggagcctat tccactgctg caagagcgcg ctgccatgca cggcttcacg
     6241 ctgaccctgc tgcccgtgac ggcacctggc gtcgagcaaa aagccacctg gctgcaagtg
     6301 cgccagaacc gtcctgacta cgtggtgctg tggggctggg gtgtgatgaa ctccaccgcc
     6361 atcaaggaag cacaggccgc cggctatgcc cgtgaaaaga tgtatggcgt gtggtgggcc
     6421 ggtgcagaac ccgatgtgaa ggacgtggct gaaggcgcca agggttacaa cgcggtgacc
     6481 atgcagcacg gatacgaacc caattccaaa gtggtgaaag acatccaggc catggtgcat
     6541 gccaagggcc agggcacagg acccaaagaa gagctgggcc aggtgctgta catgcgtggc
     6601 gccatgagcg cgatgctggg tatcgaaggt gtgcgtgctg cgcaagagcg ttttggcaag
     6661 ggcaaggtca tgaccggcga gcagattcgc tggggcctgg agaacctgaa cctgacccag
     6721 cccaagctgg atgcactcgg ttttgccggt gtgatgcgtc cggtcagcac gtcttgcacg
     6781 gaccacatgg gtgccaactg ggcccgtatc cacacctggg atggcaaggg ctggaagttc
     6841 acttccgact ggctgcaggc agacgagcaa atcctgaagc cactggtcaa gacggttgca
     6901 gccaagtatg cggccgagaa gaaactggca
//
LOCUS       DAOSJM010000086         6819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10123_length_6819_cov_6.032969, whole genome shotgun sequence.
ACCESSION   DAOSJM010000086 DAOSJM010000000
VERSION     DAOSJM010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10123_length_6819_cov_6.032969"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PKX82_04435"
     CDS             complement(<1..483)
                     /locus_tag="PKX82_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51529.1"
                     /translation="MTHPVIVIGAGLAGWTTVREFRKLDTATPVVVVTADSGDFYAKP
                     TLSNAYAQKRSPEQLVSTPAAKMVETLNVTLMVHTRVDALDVATQSITVTHDGAARTL
                     PYRQLVLATGAQPIRVPVQGNASDQVQSINALDDFKVFHSALHPYAASADSSRNERKT
                     I"
     gene            complement(480..719)
                     /locus_tag="PKX82_04440"
     CDS             complement(480..719)
                     /locus_tag="PKX82_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HOF51530.1"
                     /translation="MARFARDNMPTLKKTGSYYVMHILVAAVVAYAVTGNLWMAFTLS
                     LLEPSVQAVAFFFHEKAWMRAHAAGTGTVAPGIAA"
     gene            complement(741..905)
                     /locus_tag="PKX82_04445"
     CDS             complement(741..905)
                     /locus_tag="PKX82_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOF51531.1"
                     /translation="MKTYICIVCGFIYDETAGRPEDGIAPGTKWADVPETWECPDCGV
                     AKADFEAVEI"
     gene            complement(1007..1489)
                     /locus_tag="PKX82_04450"
     CDS             complement(1007..1489)
                     /locus_tag="PKX82_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOF51532.1"
                     /translation="MTTNQRPEIKTLANLEAAFAGESMAHIKYRYFAKIAREMGDEAT
                     ARTFEETADQEVMHAFGHLDLLYPKATMTPAKALQIAIDGETYEYSEMYPGFRATAEA
                     EGHAAAVAEMDEQIEESKVHAAQFRATLEKAQKRFAALARVEERHANHYKAQLTLIIT
                     "
     gene            1732..3042
                     /locus_tag="PKX82_04455"
     CDS             1732..3042
                     /locus_tag="PKX82_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HOF51533.1"
                     /translation="MALPLTGRAQQAYPATLAGHAVLPANSLIAAPKDAPTDLHTSGK
                     FTTGKRVDKLGSVEGLSAGRPTGVSLPFKGQPVQGHSGIKRMADGSYWLLTDNGAGSK
                     ANSPDFALYLNRYAVDFKTGTLKRLQTVFLHDPDKKVPFRIVHEGTKQRYLTGSDFDT
                     ESFQIAGGSLWIGDELGPYLIKADLKGRVQAVFETLVDGKVVRSPDHPAVTTPGVPGG
                     DVKFQVRRSKGYEGMASSPDGSKLYALLEGALWDEATKAPEMLDGKQYLRVLEFDVKS
                     EQWTGRHWKYVLDANHHAIGDFNMVSPTMGLIIERDNGEGTVDKACPEGQKRTDCFHD
                     LAKFKRVVKVELTEANVGGPLRKVGYIDLMAIADPNKLARKPLTNGVLTFPFFTIENV
                     EIVDATHIIVGNDNNLPFSSSREPNKADDNELILLEVGDFLRAQ"
     gene            complement(3088..4437)
                     /locus_tag="PKX82_04460"
     CDS             complement(3088..4437)
                     /locus_tag="PKX82_04460"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOF51534.1"
                     /translation="MKPSHRSELEAFFAQHGIRDVECLFPDMSGYPRGKLMPAHSFAT
                     GTELRIAQAIPMQCVTGDYSYDPIFPDSDPDVRLVPDYSTLKRSPWSSVPRAFAIHDC
                     LELDGQPCAFSARSLLKDVVARYTAQGLTPVVAPEIEFYLTAPNVDPSQPLTAPMARN
                     GRAEAGQSAFSLNMLNELAPFWDAFRTALDGLGVQADTWIHEVGATQYEINLMHGDPV
                     AVADQAFLFKYAAKEIAIQHGLNAVFMAKPISGAQGSSMHLHQSVVDTSGRNIFSLDD
                     GSESEAFHHYIAGLQTYVPDLMLIFAPFVNSYRRYVTNSQAPVNLQWGYDNRTTGLRI
                     PVSGPAARRVENRIAGADANPYLVIAASLAAGLAGMDEHLTPTAPLLISDNAATLGYG
                     LPRSFLPAHERMAASASARRLLGDAFVTGFCAAKGVEYDSYLYEVSAWERRFLLPQV"
     gene            complement(4522..5175)
                     /locus_tag="PKX82_04465"
     CDS             complement(4522..5175)
                     /locus_tag="PKX82_04465"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOF51535.1"
                     /translation="MRVKAWGSDVFRGGAVAAVCAATLCLGGCTTVVMETARKVYEDR
                     STDQQWTDVQIASSVLGTLSDKDKGLLLDVSADVWELRVMLTGTVVDSRTRQDLVQRV
                     RADRRILKVYDEIQVVSPEEQARRRAASNNPPGKREGAERVVGDVWVETKISAQLITT
                     GGVTSVNYRWRSVRGTVYLIGRAQTRQELATVLGIVRATDGVLQVRQFIEVRPNAKS"
     gene            complement(5150..5728)
                     /locus_tag="PKX82_04470"
     CDS             complement(5150..5728)
                     /locus_tag="PKX82_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOF51536.1"
                     /translation="MEALLISTGVVALAEIGDKTQLLAFILAARFKKPLPIILGILCA
                     TVVNHGLAGALGAWITTTLSGDTLRWVLGASFIGMAIWTLIPDKIEEEETQVAKHLGV
                     FGATLITFFLAEMGDKTQIATVAMAAKFPDPILVVIGTTLGMLIADVPAVFVGDRFAA
                     KIPMKLVHSIAAGIFAILGIATLLGAGKSLGF"
     gene            complement(5757..>6819)
                     /locus_tag="PKX82_04475"
     CDS             complement(5757..>6819)
                     /locus_tag="PKX82_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HOF51537.1"
                     /translation="ARMPGLLDGRDAVWFPFATHKGLETRVDGWLGALRARVRFGTLT
                     PEQQRDLCGPLDEMRLVKDAHEQDVMRRASQISANAHVRTMQKCAAMLRQGREVREYH
                     LDAELLHEFRRHGSQYPAYGSIVAAGANACVLHYRADAGLVRDGELVLIDAGCELDGY
                     ASDITRTFPANGKFTGPQRALYDLVLASQTAAVDATKAGARFTDPHDATVKVLAQGML
                     DLGLLDKNKVGLLSDVIEKRAYFQFYMHRTGHWLGMDVHDCGAYTEPSQIGETSERKD
                     ALTGQTIIDRPARILRPGMVLTIEPGIYVRPAEGVPEQFHHIGIRIEDDAIVTETGCE
                     LITRGVPVDADEIEALMRG"
BASE COUNT         1221 a         2118 c         2206 g         1274 t
ORIGIN      
        1 gatggtcttg cgctcatttc gagagctgtc tgcgcttgct gcataggggt ggagggccga
       61 atggaatacc ttgaagtcgt ccagcgcgtt gatggactgc acctggtctg acgcattgcc
      121 ctgcaccggc acacgaatgg gctgcgcgcc cgtggccagc accagctggc ggtacggcag
      181 tgtgcgtgcc gcgccatcgt gcgtgacggt gatggattgc gtggcgacat ccagcgcatc
      241 cacccgggtg tgcaccatga gcgtgacgtt caatgtctcc accatcttgg cggccggggt
      301 gctcaccagt tgctcggggc tgcgcttttg ggcgtaggca ttggacagcg tgggtttggc
      361 gtagaaatcg ccactgtcgg cggtcaccac caccacgggg gtggcggtgt cgagcttgcg
      421 gaactcgcgc accgtggtcc agccggccag ccctgcgcca atgacgatga cggggtgggt
      481 catgctgcga ttcccggagc cacggtgccg gtgccggccg cgtgggcccg catccaggct
      541 ttctcgtgga agaagaacgc cacggcctgg actgagggct ccagcaagct cagggtgaac
      601 gccatccaca ggttgccggt gaccgcgtag gccaccaccg cggcaaccag aatgtgcatc
      661 acgtaatagc tgccggtttt cttcagggtg ggcatgttgt cacgggcaaa gcgggccatg
      721 gtgcgctcct ttcagggggg tcagatttct acggcttcga agtcggcttt ggctacgccg
      781 cagtccgggc attcccaggt ttcgggcacg tcggcccatt tcgtgccggg ggcaataccg
      841 tcttcggggc ggcccgccgt ctcgtcgtaa atgaagccgc aaacgatgca gatgtaggtt
      901 ttcatggtgg tcctttgctt gagtgaattg tgggccgcca gcgcatacca gatatgcact
      961 ggcagctatc aaaaaagtag cttaaatcgg aaatatgaaa gcagtgttaa gtgatgatca
     1021 gcgtcaactg ggccttgtag tggttggcgt ggcgctcttc cacgcgggcc agtgcggcaa
     1081 aacgcttctg ggctttttcc agcgtggccc ggaactgggc tgcgtgcacc ttactctctt
     1141 caatctgctc gtccatctcg gccacagcag cggcatggcc ttcggcttcg gcggtggcgc
     1201 ggaaacccgg gtacatctcg ctgtactcgt aggtttcgcc gtcaatggcg atctgcagcg
     1261 ccttggctgg cgtcatggtg gccttggggt acagcaggtc gaggtggccg aaggcgtgca
     1321 ttacttcctg gtcggcggtt tcttcaaagg tacgggcggt ggcctcgtcg cccatctcgc
     1381 gggcaatttt ggcgaagtag cggtacttga tgtgcgccat ggactcgccg gcgaaagccg
     1441 cttcgaggtt ggcgagggtc ttgatttcgg ggcgttggtt cgtggtcatg gtcagctcca
     1501 ggtggtgggt tgtttgccgg tagagttttt ctatcagcac gaaacgaatg gtagcgttta
     1561 actatttaaa tgaccaatcg aaatttccta atcgattgat agtcactatg gatgtggcag
     1621 gaattcgcct ttcacaggca tgtcatctgg gccggaaata ctggggtttt cgaactggag
     1681 aaccccacca tgcatatacc taccgttctg gttaccgccc tcatcgccgc aatggccctg
     1741 cccctgaccg ggcgtgccca acaggcctac cccgccacgc tggccggcca cgccgtgctg
     1801 cccgccaata gcctgatcgc cgcgcccaag gacgcgccca ccgacctgca taccagtggc
     1861 aagttcacca ccggcaagcg cgtggacaag ctcggcagcg tggagggcct gtccgccggc
     1921 cgccccactg gcgtctcgtt gccgttcaaa ggccagcccg tgcagggcca ttcgggcatc
     1981 aaacgcatgg ccgatggcag ctactggctg ctgaccgaca acggcgcggg ctccaaggcc
     2041 aattcaccgg actttgcgct gtacctgaac cgctacgccg tggacttcaa gaccggcacg
     2101 ctcaagcgac tgcagaccgt gttcctgcac gacccggaca agaaggtgcc ttttcgcatc
     2161 gtccacgagg gcaccaagca gcgctacctg accggctccg actttgatac cgaaagcttc
     2221 cagatcgcgg gcggctcgct ctggatcggc gatgaactgg gcccgtacct gatcaaagcc
     2281 gacctcaaag gccgggtgca agctgtgttt gaaacgctgg tcgatggcaa ggtggtgcgc
     2341 tcacccgacc accctgcggt gaccacgccc ggcgtgcccg gtggcgacgt gaagttccag
     2401 gtgcgccgct ccaagggcta cgagggcatg gcgtcctccc ccgatggcag caagctctac
     2461 gccctgctgg aaggcgccct gtgggacgaa gccaccaagg cccccgagat gctggacggc
     2521 aagcagtacc tgcgcgtgct cgaatttgat gtgaagtccg agcaatggac cggccgccac
     2581 tggaagtacg tgctggacgc caaccaccac gccattggcg acttcaacat ggtcagcccc
     2641 accatgggcc tgatcatcga gcgcgacaac ggtgaaggca ccgtggacaa agcctgcccc
     2701 gaaggccaga agcgcaccga ctgcttccac gacctcgcca agttcaagcg cgtggtgaaa
     2761 gtcgagctga ccgaagccaa tgtgggtgga ccgctgcgca aggtgggcta tatcgacctg
     2821 atggcgattg ccgatccgaa caagctggcc cgcaagccgc tgaccaacgg tgtgttgacc
     2881 tttccgttct tcacgattga aaacgtcgag atcgtggacg ccacccacat cattgtgggc
     2941 aacgacaaca acctgccgtt ctccagcagc cgcgaaccca acaaggccga cgacaacgaa
     3001 ctgatcctgc tcgaagtggg cgacttcctg cgcgcccagt aagcagcgtg tagcggcaga
     3061 gtcccgcggg ggctctgccg tttttcttta tacctgcggc agcagaaagc gccgttccca
     3121 ggcgctgact tcgtacagat aactgtcgta ctccacacct ttggcggcac agaagccggt
     3181 cacaaacgca tcgccgagca gtcggcgtgc cgaagcgctg gccgccatgc gttcgtgcgc
     3241 cggcagaaag ctgcgcggca ggccgtagcc caacgtggcg gcgttgtccg agatcagcaa
     3301 gggtgcggtg ggggtgaggt gctcgtccat gccggccaga cccgccgcca gcgaggcggc
     3361 aatgactagg tacggattgg cgtcggcccc ggcaatgcgg ttttccaccc ggcgtgcggc
     3421 cgggccgctg accggaatgc gcaagccggt agtgcggttg tcgtagcccc attgcaggtt
     3481 caccggcgcc tggctgttag tgacatagcg acggtacgag ttgacgaagg gcgcgaagat
     3541 cagcatcagg tcgggcacat aggtttgcag gccggcgatg tagtggtgga aggcttcgct
     3601 ctcgctgcca tcgtccaggc tgaagatgtt gcgcccgctg gtgtccacca cgctctggtg
     3661 caggtgcatc gagctgccct gcgcacccga aatcggcttg gccatgaaca cggcattgag
     3721 cccgtgctgg atggcaatct ccttggcggc gtacttgaac aaaaacgcct ggtcggccac
     3781 cgccaccgga tcgccatgca tcaggttgat ctcgtactgc gtggcgccga cttcgtggat
     3841 ccaggtgtcg gcctgcacgc ccaggccgtc gagcgccgtg cgaaatgcat cccaaaacgg
     3901 tgccagttcg ttgagcatgt tcaggctgaa ggccgactgg cccgcctcgg ctcggccatt
     3961 gcgtgccatc ggggcggtca gcggctggct cgggtcgacg ttgggtgcgg tcaagtaaaa
     4021 ctcgatctcg ggcgccacca ctggcgtgag gccttgcgcg gtgtagcgcg ccaccacgtc
     4081 tttcaacaaa gagcgagcgg aaaaagcgca gggctggccg tccagctcca ggcagtcgtg
     4141 gatggcaaag gcgcgtggca cgctggacca cggtgagcgc ttgagcgtgc tgtagtcggg
     4201 caccagccgc acatcggggt cggagtcggg aaagatcggg tcgtaggaat agtccccggt
     4261 tacgcactgc ataggaatcg cttgcgcaat gcgcagctcg gtgccggtcg caaagctgtg
     4321 ggcgggcatg agcttgccac gggggtagcc cgacatgtcg gggaacaggc actccacatc
     4381 ccggatgcca tgctgggcaa agaaagcttc gagttcgctg cggtgggagg gtttcatacc
     4441 gccgatttta ggccgaattg gcctctagcc cccgttcgca ctgctcagat agctatcaat
     4501 ttcatagcta tctgcagccg gtcaggattt tgcgttgggg cgcacttcta tgaactggcg
     4561 cacctgcagg accccatccg tcgcccgcac gatgcccagc acggtggcga gttcctggcg
     4621 cgtctgcgcc cggccaatca ggtagaccgt gccgcgcacg ctgcgccagc ggtagttcac
     4681 cgaggtcacg ccaccagtgg tgatcagctg ggccgaaatt ttggtctcga cccacacatc
     4741 gcccaccacg cgctcggccc cttcgcgctt gcccggagga ttgttgctgg ccgcgcgccg
     4801 gcgcgcctgc tcttccggtg agacgacctg gatttcgtca tacaccttca ggatgcgccg
     4861 gtctgcgcgg acgcgctgca ccaggtcctg gcgtgtccgg ctgtccacca cggtgccggt
     4921 cagcatgacc cgcagctccc agacgtcggc actgacatcg agcagcagcc ccttgtcctt
     4981 gtcgctcaag gtgccgagca cgctgctggc aatctgcacg tcggtccact gctggtcggt
     5041 ggagcggtct tcgtagactt ttcgggcggt ctccatcacc acggtcgtgc agccccccag
     5101 gcacagggtg gccgcacaca ccgccgcaac ggccccgccc cggaacacgt cagaacccca
     5161 ggcttttacc cgcacccagc agcgtggcga tgcccagaat cgcaaagatg ccggcggcaa
     5221 tcgaatgcac cagcttcatc gggatcttcg cggcaaagcg gtcgccgaca aacacggcgg
     5281 gcacgtcggc aatcagcatg cccagcgtgg tgccgataac caccagaatc gggtcgggga
     5341 atttggcggc catggccacc gtggcaatct gggtcttgtc gcccatctcg gcgaggaaga
     5401 aggtgatcag cgtggcgccg aacacgccca ggtgtttggc gacctgggtt tcctcttcct
     5461 cgatcttgtc gggaatcagg gtccagatcg ccatgccgat gaaagacgcg cccagcaccc
     5521 agcgcagtgt gtcgccgctc agcgtggtgg tgatccacgc acccagggca ccggccaggc
     5581 cgtggttgac cacggtggcg cacagaatgc ccaggatgat gggcaacggt ttcttgaatc
     5641 gcgccgccag gatgaaagcc agcaattgcg tcttgtcgcc gatttcggcc agggcgacaa
     5701 cgccggtgga gatgagcaag gcttccatac aagggccaga gtgaggggag gcgcgattat
     5761 ccgcgcatca gcgcttcaat ttcgtcggca tccaccggca caccgcgcgt gatcagctcg
     5821 cagccggtct cggtcacgat ggcatcgtct tcaatgcgga tgccgatgtg gtggaactgc
     5881 tccggcacgc cttcggccgg gcgcacatag atgccgggct caatggtgag caccatgccg
     5941 ggacgcagga tgcgcgcggg ccggtcgatg atggtctggc ccgtgagcgc gtctttgcgc
     6001 tcgctggttt caccaatctg gctgggctcg gtgtaggcgc cgcagtcgtg cacatccatg
     6061 cccagccagt ggcccgtgcg gtgcatgtag aactggaagt aggcgcgctt ctcgatcaca
     6121 tcagaaagca ggccgacctt gttcttgtcg agcaggccca gatccagcat gccctgcgcc
     6181 agcaccttga cggtggcatc gtgcgggtcg gtaaagcggg cgccggcttt ggtggcgtcc
     6241 accgcggcgg tttgcgaagc cagcaccagg tcgtacagcg cgcgctgcgg accggtgaac
     6301 ttgccgttgg ccgggaaggt gcgggtgatg tcgctggcat agccgtcgag ctcgcagccg
     6361 gcgtcaatca gcaccagttc cccatcgcgc accagtcctg catcggcccg gtagtgcagc
     6421 acgcaggcat tggcaccggc cgccacgatg gaaccgtagg ccgggtactg cgaaccgtgg
     6481 cggcggaatt cgtgcagcag ctcggcgtcc aggtggtatt cgcgcacctc gcggccctgg
     6541 cgcagcatgg cggcgcattt ctgcatggtg cgcacatggg cgttggcgct gatctggctg
     6601 gcgcggcgca tcacgtcctg ctcgtgcgca tccttgacca ggcgcatttc gtccagcggg
     6661 ccgcacaagt cgcgctgctg ctcgggcgtc agcgtgccaa agcgcacgcg ggcgcgcagc
     6721 gcgcccagcc agccgtccac acgggtttcc agccccttgt gggtggcaaa cggaaaccac
     6781 acggcatcgc ggccatcgag caggcctggc atgcgtgcg
//
LOCUS       DAOSJM010000087         6768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10219_length_6768_cov_4.771339, whole genome shotgun sequence.
ACCESSION   DAOSJM010000087 DAOSJM010000000
VERSION     DAOSJM010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10219_length_6768_cov_4.771339"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PKX82_04480"
     CDS             complement(<1..277)
                     /locus_tag="PKX82_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOF51538.1"
                     /translation="MKFLDHLEEWLVTFLMGTATLIIFVSVAHRYAAGFAIPGVQDWL
                     LSINLTWAQELTIIMFVWMAKFGAAYGVRTGIHVGVDVLINRLDDAMR"
     gene            complement(305..1324)
                     /locus_tag="PKX82_04485"
     CDS             complement(305..1324)
                     /locus_tag="PKX82_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOF51539.1"
                     /translation="MTSSFRRTLLQASLLATSLFAGAVYAQAPIVIKFSHVVAADTPK
                     GKASEYFAKKAAELTKGKVKVEVYANSALYKDKEEMEALQIGAVQMLAPSLAKFGPLG
                     VKEFEAFDLPFIFDDRDDLHKITQGPVGASLLAKLEPKGIKGLAYWDNGFKAFSANTP
                     LKTPADFKGKKFRIQSSKVLEEEIRSVGGIPQVMAFSEVYQALQTGVVDGTENPLSNF
                     YTQKMHEVQKHLTLTNHGYLGYAVIVNKKFWEGLPGDVRGQLEQAMKQATFYANQIAE
                     DENNQSLEGVKKSGKTTIYTPTKDERLALKKAMVPVHTKMADRVGKETLQNIYKETGF
                     DPAKL"
     gene            1487..3469
                     /locus_tag="PKX82_04490"
     CDS             1487..3469
                     /locus_tag="PKX82_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF51540.1"
                     /translation="MFSVPDSVVEPHAGAVGGGRARRSLWVVPLVASIAFVTGVLVWA
                     YRTEMDEQTERRSTMIADALSSEAQLRARLDTEMARLEDLGRHLATVPRNAAGLAGDP
                     VVVEGLRGLWLSVTWLDSNNRIVAHVPDQQPLSGLRIKDLVDGPGVSSHLTLQAGGER
                     LIVRYSPALLLRRGIPWWLTRKYDVQLVDASEQVIASVDEVPLRLESDGRLSYMVLVG
                     GNMPGAYLELTSRELPLPLWRTLPLVLIGGFLALMLISTVLLRRQVRQITRAQDAWRT
                     EAAWRQAMGDSALVGLRARDLEGRILYVNRTFCDMVGLPAQVLVGLMPPMPYWPPEVA
                     DEMALRNQRNLEGHAPREGYESLWRHQDGRLINVMVFESPLIDARGKQTGWMGSIIDI
                     TARKQLEERERHQAEAMAYQSRLTTLGEVASALAHQLNQPLTAIMGYNGGLQRMLGNP
                     GYDVDAVRNALKNQGDQAAEAGRIVRRIREFLTRRGPQREACQLDAIARRAVELVQRD
                     LKTRHIQVRWALAEDLPAVYADPILVEQVLINLVRNAADAMGVAGHGGNVRIASASAG
                     QGFVRLDVEDDGPGLADRTIEQLSAPFYSTKADGMGMGLAICRSVIEVHHGGMDAGKS
                     SLGGARFSFTLPVFDPVLNEESLAGEDARAAWENME"
     gene            3466..4083
                     /locus_tag="PKX82_04495"
     CDS             3466..4083
                     /locus_tag="PKX82_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF51541.1"
                     /translation="MTLTTSPAAVHLVDDEAPVRAAMEFLLRSHGLAVFSYDSGPAFL
                     ERLGQPVPVRGCIALDVRMEPMSGLQVHDELVARGVAVPVIFLTGHGDIRMAVDALKK
                     GAFDFVEKPFGDGVLVERIHDAMAADAQQQARLADGDARGAKLAGLTQREQDVMRRVA
                     AGKLNKIIADEMNVSMRTVEVYRARVYAKLGVRSAAEVATLLARP"
     gene            complement(4092..4556)
                     /locus_tag="PKX82_04500"
     CDS             complement(4092..4556)
                     /locus_tag="PKX82_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOF51542.1"
                     /translation="MLCGLVADLLLKSDWKLATAESCTGGLIAAACTELSGSSNWFER
                     GFVTYSNEAKTELLGVDAALIARHGAVSEEVARAMAQGALQRSHAQVALSVTGVAGPT
                     GGSAAKPVGTVWFGWATPSGVTTDMRLFAGDRAAVRAAAVHHALHTLHTLLT"
     gene            complement(4586..5167)
                     /locus_tag="PKX82_04505"
     CDS             complement(4586..5167)
                     /locus_tag="PKX82_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOF51543.1"
                     /translation="MIDPANSPVTVFPVDPGQPAPIQRPSMRFMLSHPAHAIALGFGS
                     GLSRIAPGTAGTLWAWLSFVVLSQWLDDTGWGIAIALSLPLGWWASTVTARNMGVLDP
                     GSIVWDEVAAFWLVLWLVMPTGLVGQVLAFGLFRFFDAVKPGPVGWADRLFHHVDPNT
                     DPHAWSRAGFGIMLDDLVAAGCTVLVVALWRFV"
     gene            complement(5164..6078)
                     /locus_tag="PKX82_04510"
     CDS             complement(5164..6078)
                     /locus_tag="PKX82_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF51544.1"
                     /translation="MQLQLDDVALFARIAELGTLSAAARERDVPVSQVTRALARLEAH
                     CGVRLLHRTTHGLSLTDEGDTFLVYARRLLDTTAELDSELTGKLSGPSGWVRISVSPV
                     IADMLIAPTLPGLYARYPGLHLEINADDRMADMARDGIDIAIRTGQVTQDTLVARHIG
                     SFGRTLVASPDYLERHGTPRCVDDLRHHHLITNSANPLVNRWPIQGRAPQAYFTAQGA
                     TRADNSALILMLARSGAGVGRVMDLTALPLIRSGELVPVLQDAIDPQTVPIVAVMLQE
                     RQRLPKIRACLDYWAECIGALGTPIIPV"
     gene            6207..6434
                     /locus_tag="PKX82_04515"
     CDS             6207..6434
                     /locus_tag="PKX82_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51545.1"
                     /translation="MFHPLPDADLQARLHDLAKLEAARLRHEAIDDFWRGADAVWRRS
                     LGAMAQRSAARLRARLVRCAGGVPSGQPSGV"
     gene            6436..>6768
                     /locus_tag="PKX82_04520"
     CDS             6436..>6768
                     /locus_tag="PKX82_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HOF51546.1"
                     /translation="MPTLLLKVSPLQNPSRYAALAQALTCITADTLGKRAEVTAVVID
                     DLPAARWYVGGSAVARATALLEISITQGTNTVEEKSAFIATAYAELERQLGVGQPLEP
                     ASYVIVREV"
BASE COUNT         1163 a         2011 c         2307 g         1287 t
ORIGIN      
        1 tgcgcatcgc atcgtccagg cggttgatga gtacgtccac gcccacgtgg atgccggtcc
       61 gcaccccata ggccgcgccg aacttggcca tccacacgaa catgatgatg gtgagttcct
      121 gggcccaggt cagattgatg gacaggagcc agtcctgcac gccggggatg gcgaagccgg
      181 cggcatagcg gtgggcgacc gagacaaaga tgatcagtgt cgcggtgccc atgaggaagg
      241 tgacaagcca ctcttccaga tggtcaagaa atttcatgaa attgcctgtg cagtcgacga
      301 gggtttacag cttggccgga tcaaagccgg tttccttgta gatgttctgc agggtttcct
      361 tgcccacgcg gtcggccatc ttggtgtgca cgggcaccat ggctttcttc agtgccaggc
      421 gctcgtcctt ggtgggggtg tagatggtgg tcttgccgct cttcttcacg ccttccagtg
      481 actggttgtt ttcgtcttcg gcaatctggt tggcgtagaa ggtggcctgt ttcatggcct
      541 gttccagttg gcctcgcaca tcaccgggca ggccttccca gaacttcttg ttcacgatca
      601 ccgcatagcc caggtagcca tggttggtga gcgtgaggtg tttctgcact tcatgcatct
      661 tctgggtata gaagttggac agcgggtttt ccgtgccgtc gaccacaccg gtctgcaggg
      721 cctggtaaac ctcggagaac gccatcacct gggggatgcc acccacggaa cggatttctt
      781 cttccagcac cttcgaggac tggatgcgga atttcttgcc cttgaagtca gctggtgtct
      841 tcagaggggt gttggctgaa aaggccttga acccgttgtc ccagtaggcc aatcccttga
      901 tgcccttggg ctccagtttg gccagcaggc tggcgcctac gggaccctgg gtgatcttgt
      961 gcaggtcgtc acggtcatca aaaatgaagg gcagatcgaa cgcctcgaac tctttcacac
     1021 ccagcgggcc gaacttggcc agggacgggg cgagcatttg caccgcgccg atctggagtg
     1081 cttccatctc ttccttgtcc ttgtacaggg cactgttggc atagacctcg accttgactt
     1141 tgcccttggt cagctcggca gctttcttgg cgaagtattc cgatgccttg cctttggggg
     1201 tgtcggcggc gaccacatgg ctgaacttga tcacgatggg cgcctgcgca tacacggcgc
     1261 cggcaaatag ggatgtggcc agcagggacg cttgcaggag ggtgcggcga aagcttgatg
     1321 tcatgggatg tctccggttg aaaatggaat ggaccgcagt gtgcttctgc agggactctg
     1381 gcggctagtg tggatttcca catttgtcgc gcagcggagg tgggttttaa gggtaaaccc
     1441 ttggttttgg ggttgtaggc atgccggtgg cggctagact gtctccatgt tttctgtgcc
     1501 cgactccgtt gtagaacccc atgccggggc ggtgggcggc ggacgtgcgc ggcgcagtct
     1561 gtgggtggtg cccttggtgg cttccattgc gtttgtgacc ggggtgctgg tgtgggccta
     1621 ccgtaccgag atggatgagc agaccgagcg gcgcagcacc atgattgccg acgccctgag
     1681 cagtgaagcc cagttacgtg cccggctgga caccgagatg gcccggctgg aagacctggg
     1741 gcgccacctg gcgaccgttc cgcgcaatgc agcgggcctg gcaggggacc cggtggtggt
     1801 ggagggcttg cggggcctct ggctcagcgt gacctggctg gacagcaata accgcatcgt
     1861 ggcccatgtg ccggaccagc agcccttgtc cggacttcgc atcaaggacc tggtggatgg
     1921 ccccggtgtg tcgtcccatc tgaccctgca ggcgggtggt gagcggctga tcgtgcgcta
     1981 ctccccggcg ctgctgctgc gccggggcat cccgtggtgg ttgacccgca agtacgacgt
     2041 gcaactggtg gacgcctcgg agcaggtgat tgcctcggtg gatgaagtgc ccctgcggct
     2101 ggagtccgac gggcgactca gctacatggt gctggtgggc ggaaatatgc cgggggcata
     2161 cctggagttg acttcgcgtg aattgccctt gcctctgtgg cgcactttgc cgctggtgct
     2221 gatcggtggg tttctggcgt tgatgctgat ttcgacggtg ctgctgcgcc ggcaggtgcg
     2281 ccagatcacg cgtgcacagg acgcctggag aaccgaggcg gcctggcgcc aggccatggg
     2341 agattccgcc ctggtggggc tgcgggcccg tgatctcgaa ggccgcatcc tgtatgtcaa
     2401 ccggaccttc tgcgacatgg tggggctgcc cgcgcaggtg ctggtcgggt tgatgccgcc
     2461 gatgccgtac tggccgcctg aagtagccga tgaaatggcc ctgcgcaacc agcgcaacct
     2521 ggaggggcac gcgccgcggg agggctacga atcgctctgg cgccaccagg acggacgcct
     2581 gatcaatgtg atggtgttcg agtcgccgtt gatcgacgcc cgcggaaaac aaaccggctg
     2641 gatgggctcc atcatcgaca tcaccgcccg caagcaactg gaagagcgtg aacggcacca
     2701 ggccgaggct atggcctacc agtcccgcct gacaacgctc ggtgaagtgg catccgcact
     2761 ggcgcaccag ctgaaccagc cgctgacggc catcatgggc tacaacggcg gcttgcagcg
     2821 catgctgggc aaccctggct atgacgtgga cgccgtgcgc aacgccctga aaaaccaggg
     2881 tgaccaggcc gccgaggcgg gccgcatcgt ccggcgtatc cgcgagtttc tgacgcgcag
     2941 gggcccgcag cgcgaagcct gccagctcga cgccattgca cggcgcgcgg tggagctggt
     3001 gcagcgcgat ctcaagaccc gccacatcca ggtgcggtgg gctctggcag aggatttgcc
     3061 cgccgtgtat gccgacccca ttctggtgga gcaggtgctg atcaacctgg tgcgcaacgc
     3121 cgcggatgcg atgggggtgg ctggccatgg cggcaatgtg cgcatcgcct ccgccagtgc
     3181 agggcagggg tttgtgcggc tggatgtgga agacgatggt ccgggcctgg cagatcgcac
     3241 aatcgagcag ttgagcgccc ccttctattc caccaaagcc gatggcatgg ggatggggct
     3301 ggccatctgc cgctcggtga tcgaggtgca ccacggcggc atggatgcgg gcaagagttc
     3361 gctgggcggt gcacggtttt cgttcaccct gccggtgttc gatccggtgc tgaacgaaga
     3421 atcactggcg ggcgaggacg cgcgggccgc atgggagaac atggaatgac gctgacaacc
     3481 agcccggcgg cagtccatct ggtggacgac gaagcccctg tgcgtgcggc gatggaattc
     3541 ttgctgcgct cgcatgggct ggctgtgttc agctacgaca gcggcccggc ctttctggaa
     3601 cgcctggggc agcctgtgcc ggtacgcggg tgcatcgcgc tggatgtccg catggagccg
     3661 atgtccggat tgcaggtgca cgatgaactg gtggcgcgcg gcgtggcggt ccccgtcatt
     3721 tttttgaccg gccacggcga catccgcatg gccgtggacg ccctgaagaa gggcgccttc
     3781 gactttgtcg agaaaccttt tggcgacggt gtgctggtgg agcgcatcca cgatgcgatg
     3841 gcagcggatg cccagcagca ggcccggctg gccgatggag atgcgcgtgg cgccaagctg
     3901 gccggactga cgcaacgtga gcaggacgtg atgcgccgtg tggcagctgg caagctcaac
     3961 aagatcattg ccgatgagat gaatgtttcc atgcgcacgg tggaggtcta ccgtgcccgt
     4021 gtctatgcca agctcggggt gcgttcagcc gccgaggtgg ctaccctgct ggcacggccc
     4081 tgagcgctgg ctcaggtcag cagcgtgtgc agggtgtgca atgcatggtg cacggcggcc
     4141 gcgcgcacag cagcgcggtc gcccgcaaac aggcgcatgt cggtcgtcac gccgctgggg
     4201 gtggcccagc cgaaccagac ggtgcccaca ggtttggcag cgctgccgcc ggtggggccg
     4261 gcgacgccgg tgaccgacag cgccacctgc gcatgggagc gttgcaaagc cccttgtgcc
     4321 atggcgcgtg ccacctcttc actgactgcg ccgtggcgtg cgatcagagc ggcatcgacg
     4381 ccgagcagtt cggtctttgc ttcgttggaa taggtgacaa aaccgcgttc aaaccagttg
     4441 ctggaacccg aaagctccgt gcacgcagca gcaatcagac caccggtgca actctcggca
     4501 gtggccagct tccaatcgga tttaagaagc aaatcagcca ccagtccgca cagaatatgc
     4561 gtggatagct cctctttttg tagcgtcaca cgaacctcca gagagccacc accagcacgg
     4621 tgcagcctgc cgccaccagg tcgtcgagca tgatgccaaa gcccgccctg gaccaggcgt
     4681 gcgggtcagt gttcggatcg acgtggtgga acaagcggtc ggcccagccc accggccccg
     4741 gcttgaccgc atcaaagaag cggaacaggc caaacgccag cacttgcccg accagcccgg
     4801 tgggcatgac cagccacagc accagccaga acgcggccac ctcgtcccac acgatgctgc
     4861 ccgggtccaa cacgcccatg ttgcgggcgg tcacggtgct ggcccaccag ccgagcggca
     4921 gcgaaagcgc aatggcgatg ccccagccgg tgtcgtccag ccattgggaa agcaccacga
     4981 aagacaacca ggcccacagc gtgcccgcgg tgccgggggc aatgcgcgag aggccagagc
     5041 caaagcccag cgcaatcgcg tgggccgggt gcgagagcat gaagcgcatc gacggccgct
     5101 gaatgggggc gggttgtccg gggtcgacgg gaaagacggt gacgggcgaa ttcgcgggat
     5161 caatcatacg gggattatcg gtgtgccaag ggcgccaatg cattcggccc agtagtccag
     5221 gcaggcgcgg attttgggca ggcgctggcg ttcctgcagc atcacggcca cgatggggac
     5281 ggtctgcgga tcgatggcgt cctgcagcac gggcaccagt tcgccactgc ggatcagtgg
     5341 cagggcggtc aggtccatga ctctgcccac gcctgcgccg ctgcgtgcca gcatcagtat
     5401 cagtgccgag ttgtccgccc gtgtggcgcc ctgtgcggtg aagtacgctt gcggcgcacg
     5461 cccttggatt ggccatcggt tgaccagcgg gttggcgctg ttggtgatca ggtggtggtg
     5521 ccgcaagtcg tccacacagc gcggcgtgcc atggcgctcg aggtagtcgg gagatgccac
     5581 gagcgtgcgc ccgaagctgc cgatgtggcg ggctaccagc gtgtcctgtg tgacctgccc
     5641 ggtgcgtatc gcgatgtcga tgccatcgcg ggccatgtcg gccatgcggt cgtccgcatt
     5701 gatctccagg tgcaggccgg gatagcgtgc atacaggccg ggcagggtcg gggcaatcag
     5761 catgtcggca atcaccgggc tgacgctgat acgcacccag ccactgggcc cactgagttt
     5821 gccggtcagt tcgctgtcca gctcggccgt ggtgtccagc aggcgtcggg catagaccaa
     5881 aaaagtgtcg ccttcatcgg tcaggctcag tccgtgggtt gtgcggtgta gcaggcgcac
     5941 gccgcagtgc gcttccagtc gcgccagcgc gcgggtgacc tgactgaccg gcacatcgcg
     6001 ttcgcgggcc gctgccgaca gcgtgcccag ctcggcgatg cgggcgaaca gggcgacatc
     6061 gtccagttga agttgcatgg tggcattgtg cgctggattg aacttgtttt gcaaataatg
     6121 caataccaat atggatttgc tgctgtttcc ggaagttgga gcattcaata aagttcattc
     6181 catcgttcca cttcacggag ttcaccatgt tccatcccct tcctgacgcc gacctccagg
     6241 cccgcctgca tgatctggcc aagttggagg ccgcccggtt gcggcatgag gccatcgacg
     6301 atttctggcg tggtgccgac gccgtatggc ggcgcagcct gggcgctatg gcccagcgca
     6361 gtgcggcgcg cctgcgagcc cgtctggtcc gctgcgccgg cggtgtgccg tctggccagc
     6421 catcgggagt ctgagatgcc caccttgctg ctcaaagtct ctccgttgca aaacccgtcg
     6481 cgctacgcgg cactggcgca ggcgctgacc tgcattaccg cagacaccct gggcaagcgt
     6541 gccgaggtga ctgcggtggt gattgacgac ctgcctgcgg ctcgctggta tgtgggtggc
     6601 agtgcagtgg cgcgggccac cgccctgctg gaaatcagca tcacgcaggg cacgaacacg
     6661 gttgaagaaa agtcggcttt cattgccacg gcctatgccg agctggagcg ccagctgggc
     6721 gttggccagc ccctggagcc tgccagctac gtgatcgtgc gcgaagtg
//
LOCUS       DAOSJM010000088         6760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10236_length_6760_cov_4.847875, whole genome shotgun sequence.
ACCESSION   DAOSJM010000088 DAOSJM010000000
VERSION     DAOSJM010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10236_length_6760_cov_4.847875"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1115)
                     /locus_tag="PKX82_04525"
     CDS             complement(72..1115)
                     /locus_tag="PKX82_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOF51547.1"
                     /translation="MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEALDH
                     KQVHAMVYDIMNDGQRKHYEEFLEIDFSFEIDGLARFRVNAFNHQRGAGAVFRTIPSK
                     ILSLEQLNAPKIFADLALKPRGMVLVTGPTGSGKSTTLAAMVNYLNETEFGHILTVED
                     PIEFVHESKKCLVNQREVGPHTLSFAAALKSALREDPDAILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPADEKEMVRAMLSESLQAVISQTLCKTKDGQG
                     RVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQNLTDLVRRNIISAAE
                     ARSKAKIPENFAG"
     gene            1160..1858
                     /locus_tag="PKX82_04530"
     CDS             1160..1858
                     /locus_tag="PKX82_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOF51548.1"
                     /translation="MTMIADNLQQVRTRIATACNTHGRPPEAVTLLAVSKTFGPQAVR
                     EAFAAGQTAFGENYIQEAVDKIVALADLPLEWHCIGPIQSNKTRLVAEHFDWAHSVDR
                     LKIAQRLSEQRPKGTAPLNVCIQVNIDGGPTKAGVAPEDALALAQAVVALPNVRLRGI
                     MTIPEPAIDFEAACAVHMRARALFDALNAAGLVLDTLSMGMTGDLEAAIASGSTMVRV
                     GTAIFGGRTYPTRV"
     gene            complement(1855..2982)
                     /locus_tag="PKX82_04535"
                     /pseudo
     CDS             complement(1855..2982)
                     /locus_tag="PKX82_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903920.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(3075..5645)
                     /locus_tag="PKX82_04540"
     CDS             complement(3075..5645)
                     /locus_tag="PKX82_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF51549.1"
                     /translation="MATLFSNFTPTLGHSRLRWFGCAWLFWLLLNTSALAAPREVRVG
                     VYQNEPKIFMGSDGQPSGILGELLVEMAKREGWTLRPVRCEWQACLDALKDGKLDLMP
                     DVAFNEQRDTVFDFHKVPALLSWSQIYKHSGITINSALDLRDKRIAVLEGSVQQSYLR
                     DLLSGFGVQAELVPVKSLGEGFDLATSGSVDAAAANRFFGELQAPRYKLAATPILFQP
                     SQLFYATSSGRNADLLAAIDTHLTEWGASPDSHYFQILKRWMATPPQFAVPRYVTWTL
                     AALVLALLIAVGVGVLLRREVARKTAHIQAGEDRLATILNRVDAFIFIKGPDLRYQYA
                     NRKACDLFGITQDEIIGRTDADFFDPDTAQKLQHNDLRVLRDGERVEEEETNLRANGT
                     EARTYLSIKLPLRDAQGLIYALCGISTDLTRHKQTEQAIHKLAFYDPLTQLPNRRLLL
                     ERIQSALLAHSRDHQNGALLFIDVDNFKDLNDTLGHQMGDELLRQMAHRLTACIRAQD
                     TLARQGGDEFVVMLQGLSPSMPEAVQQARQVGDKILQRLQEPYALEQQQYNSSVSIGV
                     AMMEPGTSNQDTLMQQADLAMYRAKEEGRNTVRFFDPQMQALVSQRTALEADMRSGIS
                     AGEFVLHYQPQVDAQGQVFAVEALARWNHPKRGLVPPSQFIGVAESSGLILPLGEWLL
                     DAACRQLVSWAEIPEMAHRFIAVNVSARQFKQPHFVQFIESALGTTGAPAGRLELELT
                     ESLLIEDVAGVVAKMNALKALGVRLSLDDFGTGYSSLGILKRLPLDQLKIDQGFVRDL
                     LTDAQDASIVRAIISLGESLQLQVIAEGVETEAQRDALLALGCTHFQGYLFGRPAPMV
                     "
     gene            5819..>6760
                     /locus_tag="PKX82_04545"
     CDS             5819..>6760
                     /locus_tag="PKX82_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51550.1"
                     /translation="MHQNLSLHERLKRPAVQWPIWTGLMLVMGLLIGVVQWREYREIG
                     RTEREHLLTQVRVADQLLTSQIQSADAALRTMLEGLDRWRGPEGYLPFAHEHLKRVEK
                     MMPGARTFAVLDAQGICQLSNRHELIGMDLSGRDYFARAKAGHGSAELRIAAPYRTVL
                     GACAATISRPITRADGTFGGVVVATLSPEYFEDVMKTLRYAPDMRVTLLHDEGQIYVT
                     SPPDQAILARDYSKTSNFFSEHLRSGLAESYQGGNSLDERKRFGMVRTVALGGIAADH
                     RFVAVAGRESSAVYGPWRMETAVLVGLWLAILLGSGWG"
BASE COUNT         1254 a         1999 c         2158 g         1349 t
ORIGIN      
        1 cagcttgagc aggtcggtaa tgaaggtggt ggcttgatcg cgttccatga caactccttg
       61 gtctagtggg gtcagcctgc aaaattctct ggaatcttgg ccttggatcg ggcctcggcc
      121 gcgctgatga tgttgcgccg aaccaggtca gtcagatttt ggtccagcgt ctgcatgccg
      181 acactgttgc cggtctggat gctggagtac atctgcgcca ccttggcctc gcggatcagg
      241 ttgcgaatgg cactggtgcc cagcatgatc tcgtgggcag cgacgcggcc ctggccatcc
      301 ttggtcttgc acagggtctg cgaaatcacg gcctgcagcg attcggacag catggctcgg
      361 accatttcct tttcgtcggc cgggaacacg tcaatgatcc ggtcgatggt tttggcggcg
      421 ctggaggtgt ggagtgtgcc aaacaccagg tggccggttt cggctgctgt catggccagg
      481 cggatggttt ccaggtcgcg catttcgccc accagaatcg catccgggtc ttcgcgcagc
      541 gcagacttca gcgccgcagc gaacgacagc gtatgcggcc ccacttcgcg ctggttgacc
      601 aggcacttct tggactcgtg cacaaactcg atcgggtctt ccaccgtcag gatgtggccg
      661 aactccgtct cattgaggta gttcaccatc gctgccagcg tggtggactt gcccgaacct
      721 gtggggccgg tgaccagcac catgccgcgg ggcttgaggg ccaggtcggc aaaaatcttg
      781 ggtgcgttga gttgttccag cgaaagaatc ttgctgggaa tggtccggaa cacggcgccg
      841 gcaccacgct ggtggttgaa ggcgttgacg cggaagcggg cgagtccatc gatctcgaat
      901 gaaaagtcga tttccaggaa ttcttcgtag tgcttgcgct ggccgtcgtt catgatgtcg
      961 tacaccatgg cgtgcacctg tttgtggtcc agtgcttcga cgttgatgcg tcgcacgtcg
     1021 ccatgcacac ggatcatggg cggcaggccg gcggacaggt gcagatcgga tgccttgttc
     1081 ttgacgctga aggccagcaa ttgggtaatg tccacaaagt cctcttggaa tggttcgttg
     1141 tttgttacgc tcgtagatta tgaccatgat tgccgacaat ctccaacagg tacgcacccg
     1201 gattgcgacc gcgtgtaaca cccacggacg cccacccgaa gcggtgactt tgctggcggt
     1261 ttccaagacc ttcggaccgc aggcggtgcg cgaggccttc gccgccggcc agacggcgtt
     1321 tggtgaaaac tacattcagg aggcggtcga caagatcgtc gcactggcgg acctgccact
     1381 ggagtggcac tgcattggcc cgatccagag caacaagacg cgactggttg ccgaacattt
     1441 tgactgggcg cattcggtag accggctcaa gatcgcccag cggctcagcg aacagcgacc
     1501 caagggtaca gcgccgctga acgtctgcat ccaggtcaac atcgatggcg gaccaaccaa
     1561 ggccggtgta gcgccagagg acgccctggc gctggcccag gccgtagtgg cgttgcccaa
     1621 cgtgcgcttg cgcgggatca tgaccattcc cgagcctgct attgattttg aagcagcttg
     1681 cgcagtacac atgcgggcta gagctctatt tgatgcacta aatgcagcag ggctggtact
     1741 cgacaccctg tccatgggca tgacgggtga tctggaagcc gccatcgcct cgggcagcac
     1801 catggtgcgg gtgggtaccg ccatttttgg cggtagaacc tacccgacgc gggtttaggt
     1861 tcgcacccgt ccgtcgccgg tcagcatgga cagctccaca aagctggagg ctacgtggtc
     1921 ggtcggggaa aagtggacgt tgaatccacc aaaagactgg ttgctgatgc tctccagtcc
     1981 ggcgatcagc ccgtcgcgcc cgcctttggc actgctgcgc cgcaggcctt cggccatcac
     2041 cttggctgct acatagcctt ccatgctgga gaagttggcc gacaccgcgc cattgctctt
     2101 gcgcaccatc tcgataaatt cgcgcgaaat gcccttggcg ttggtgtagg gcgtgggcat
     2161 cacctgcgac accaccacgc cggcaccctc cttgcccagc tcatcggcca gggcctgtgt
     2221 gccgacaaag gacacgttgt aaaagccacc accgtagccc gctttacggg cggcccggat
     2281 ataggctgcg cacgacttgt aggcgctgat ctgtaccacg gcttccggtg ccgctgcact
     2341 gatggtttgc accgccttgg ccacatccac ggaattgcgc tccacggtgg cgacggctac
     2401 aggcttgagc ttgagggcct ccagcgccag cgtgacacca tcgaggcccg ccttgccgta
     2461 ggcatcgttc tggtagaaca ccgccacttt ggtgatgcct acgttgtgca actacctgac
     2521 gatcagcccg gtttcgtcgt tgtaggacgc gcgcacatga aatgcgtagc gattcagcgg
     2581 ggcgcgcagt gccatggcgc cggtaaacgg ggcaacaaac ggcaccctgg ccttggtggc
     2641 cagcggcagc gccgcaatgc tggtgggggt gccgatgtag ccaaacaggg caaagacgtc
     2701 gttatcgatc agtttgcgcg tgttttctgc gcagcggtcg ggctcatagc cgtcgtccat
     2761 ctgcacaatt tctacattgc gtccgcccac gccaccctgg gtattgagtt ggtcaaagaa
     2821 cagtttggca ccggcgtgga actggatgcc cagctgagcc gccgggcctg taaaggcagc
     2881 cgactgtccg agcacgatgc gatccgactg ggcgtgcact gccggaaaac cgaccacgct
     2941 ggcagcacca gcggcgagag aaaattggcg acgattgatc atgggcatat tgtggtgcgg
     3001 ttcaccgggt ttgcgaaggg aaaagtgtta cagaagttag aggtgggtgg tgttctgcgg
     3061 gtatcttgca tgggctagac catcggggcc ggacggccga acagatagcc ctggaaatgc
     3121 gtacaaccca gcgccagcag cgcgtcgcgt tgcgcttcgg tttccacacc ttcggcaatg
     3181 acttgcagct ggaggctttc accaagcgaa atgatggccc gtacgatgct ggcgtcctgc
     3241 gcgtcggtca acaggtcgcg cacgaaaccc tggtcgatct tgagctggtc cagtggcaag
     3301 cgcttgagga tgcccagcga ggaatatccg gtaccaaaat catccagcga cagccgcacg
     3361 cccagggcct tgagggcatt catcttggcg accacgccag caacatcttc aatcagcagg
     3421 ctctctgtca gttccagttc caggcgcccg gccggggcgc ccgtggtgcc gagcgccgac
     3481 tcgatgaact gcacaaaatg gggctgcttg aactggcgtg cgctgacgtt gaccgcaatg
     3541 aagcggtggg ccatctcggg aatctctgcc cagctcacca gctggcggca ggcggcatcc
     3601 aggagccatt cgcccagcgg caggatcagc cccgaactct cggccacgcc aatgaactga
     3661 gacggcggta ccaagccgcg cttcggatga ttccagcgtg ccagcgcttc caccgcgaac
     3721 acctggccct gtgcatccac ctggggctgg tagtgcagga caaattcccc ggcggaaatg
     3781 ccggagcgca tgtcggcctc cagggcggtg cgctggctga ccagggcctg catctgcggg
     3841 tcaaaaaagc gcactgtgtt gcgcccttcc tccttggccc ggtacatcgc cagatcggcc
     3901 tgctgcatca gggtgtcctg gttggaagtt ccgggttcca tcatggccac gccaatactg
     3961 acgctggagt tgtactgctg ttgttccagt gcatagggtt cctgcaggcg ctgcagtatc
     4021 ttatcgccca cctggcgtgc ctgctgcacg gcctccggca tggagggact cagcccctgc
     4081 agcatgacca caaattcgtc gccgccctgg cgggccaggg tgtcctgcgc ccggatgcag
     4141 gcggtgagcc ggtgggccat ctgacgcagc agttcgtcgc ccatctggtg gcccagcgta
     4201 tcgttgaggt ccttgaagtt gtccacatca atgaacagca gagcgccatt ctggtggtcg
     4261 cggctgtggg ccagcaaggc ggactgtatg cgttccagca acagcctgcg gttcggcagc
     4321 tgggtcagcg ggtcgtagaa cgccagcttg tggatggctt gctcggtctg cttgtggcgg
     4381 gtgaggtccg tggaaattcc gcacagggca taaatgaggc cctgcgcgtc gcgcaacggc
     4441 agcttgatcg acaggtaggt gcgtgcctcg gttccgttcg cacggagatt ggtctcctcc
     4501 tcttcgacgc gctcaccatc ccgcagaacg cgcaggtcat tgtgctggag cttctgcgcc
     4561 gtgtctgggt caaaaaaatc ggcgtcggtc cggccaataa tttcgtcctg ggtgatgccg
     4621 aagaggtcgc acgccttgcg gttcgcatac tgatagcgca gatcggggcc cttaatgaag
     4681 atgaaggcgt ccaccctgtt gagaatggtg gccagccggt cctcgccggc ctggatgtgc
     4741 gcggtcttgc gggccacctc gcggcgcaac agtacgccca cgccgacggc gatcagcagc
     4801 gccagcacca gggccgccag cgtccacgtg acatagcggg gaactgcaaa ctgcggaggc
     4861 gtcgccatcc agcgcttgag aatttgaaag tagtgggagt cgggcgatgc cccccattcg
     4921 gtcaggtggg tgtcaatggc cgccagcaaa tcggcattgc ggccactgct ggtggcatag
     4981 aacagctgcg agggctggaa caggatgggg gtggccgcca gcttgtaccg tggcgcctgg
     5041 agttcaccaa aaaagcggtt ggctgcagcg gcgtccacgc tgcccgaagt cgcaagatca
     5101 aacccctcgc cgagcgactt caccgggacc agttccgcct gtacgccgaa tccggacagc
     5161 aggtcgcgca ggtagctctg ttgcaccgaa ccttcgagta ctgcgatgcg cttgtccctg
     5221 aggtccagtg ccgaattgat ggtgatgcca ctgtgcttgt aaatctgcga ccagcttagc
     5281 agcgcaggga ctttgtggaa gtcaaacacc gtgtcccgct gttcgttgaa ggccacatcg
     5341 ggcatcaggt ccagtttgcc gtctttcagt gcatccaggc aggcttgcca ctcgcagcgg
     5401 accgggcgca gcgtccagcc ttcacgtttc gccatctcca ccagcaactc accgaggatg
     5461 ccactgggct gcccgtcgga gcccatgaag atcttgggtt cgttctggta cacccccaca
     5521 cgcacttcgc gtggggcggc caaggcgctg gtgttcagca gcagccagaa cagccacgca
     5581 catccgaacc agcgcaggcg gctgtggccc agtgtgggag tgaagttgga gaacagcgtg
     5641 gccatgggat gttcaaagct aacggtgatc cgccattgtc ctgcatgaac cgccatgttg
     5701 gaacgccagt tttctgttat cgaagcaatg gccactggtt cgggggacta tgccgcattc
     5761 gggtgcatac ccccacccga atgggttata aatgggaggc attccctgct gaccgagcat
     5821 gcaccagaac ctctctcttc acgaacgcct gaagcgtccc gccgtgcagt ggcccatctg
     5881 gacggggctg atgctggtaa tgggcttgct gattggtgtg gtgcagtggc gtgaataccg
     5941 ggagatcgga cggactgagc gggagcacct gttgacccag gtgcgggtgg ccgaccagtt
     6001 gctcacgtcc cagatccagt ctgccgatgc agcgctgcgc accatgctcg aaggcctgga
     6061 ccgctggcgc gggcccgagg gctaccttcc gtttgcccat gagcacctga agcgggttga
     6121 aaagatgatg cccggcgcgc gcaccttcgc cgtgctggac gcgcagggca tttgtcagct
     6181 ctccaatcgg cacgaactga tcggaatgga tctgtccggg cgcgattact ttgcccgtgc
     6241 gaaggcgggc cacggaagcg cagaactgcg cattgccgcg ccctaccgca cggtgctggg
     6301 ggcctgcgcg gccaccattt cgcggccgat cacccgggcc gatggtacgt ttggcggggt
     6361 ggtggttgcg accttgtccc ccgaatattt tgaagacgtg atgaaaacgc tgcgttacgc
     6421 cccggacatg cgggtgacgc tgctgcacga cgaggggcag atctatgtga cgtcgccacc
     6481 agatcaggcg atattggccc gtgactattc aaaaaccagc aatttcttca gtgaacacct
     6541 gcgtagcggc ctggcggaaa gctaccaggg tggtaacagt ctggacgagc gcaaacgctt
     6601 tggcatggtg cgcaccgtgg cattgggcgg catagcggca gaccaccgtt ttgtggccgt
     6661 ggctgggcgg gagtcctccg cagtttatgg accctggcgc atggagacgg cggtcttggt
     6721 ggggctgtgg ctggcaattc tgctgggcag cggatggggg
//
LOCUS       DAOSJM010000089         6730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10293_length_6730_cov_4.794007, whole genome shotgun sequence.
ACCESSION   DAOSJM010000089 DAOSJM010000000
VERSION     DAOSJM010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10293_length_6730_cov_4.794007"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..957)
                     /locus_tag="PKX82_04550"
     CDS             complement(16..957)
                     /locus_tag="PKX82_04550"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOF51551.1"
                     /translation="MSAIPPSMSDRDGKIWMDGQMVDWRDAKIHVLTHTLHYGCGAFE
                     GVRAYKGDSGVTSIFRLEEHTDRLFNSAKILRMKIPFTKEQVNEAQKAVIRENKLESG
                     YLRPLTWVGDRKLGVSPKGNTIHLMVAAWPWGAYLGEEGMKRGIRVKISSYTRHHVNI
                     TMTQAKAVSNYTNSILANMEATDDGYDEAILLDASGFVSEGAGENIFVIKDGVVYTPD
                     LSAGALNGITRNTVLHICKDLGLELVQKRITRDELYISDEVFFTGTAAEVTPIREVDR
                     IEIGSGSRGPITEKIQAAFFDIVNGRNPKYAHWLSKV"
     gene            complement(1008..1400)
                     /locus_tag="PKX82_04555"
     CDS             complement(1008..1400)
                     /locus_tag="PKX82_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOF51552.1"
                     /translation="MQFQKILIPIASVVLVVAAYRAYGWAGVAAAVGALVMWMLLHFT
                     RMLQILKRAANRPVGYVESAVMLNAKLQSGATLLHVVAMTRSLGELLSPKDTQPERFR
                     WTDASQSHVTCEFVAGRLKAWELHRPAP"
     gene            complement(1414..2760)
                     /locus_tag="PKX82_04560"
     CDS             complement(1414..2760)
                     /locus_tag="PKX82_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51553.1"
                     /translation="MTAPQPPRSLQARVLTLVVGLVTLVWLAAALFTWIDASHELDEL
                     LDGHLAQAAGLLVVQQSESEGDDDGVLDAPSLHKYAPKVAFQVFHEGILITRTTNASE
                     QPMSDKTTGFSTRKLGDGKEWRVFGARGAERDVQVFVGERTESRNDILWAVMRGLLLP
                     LVLALPLLAAALWWSVRAGLSPLRALSEALAQRAPQTLEPVVLDDCPEEMRPLVGALN
                     ELLERIGRMVVSERRFTADAAHELRTPIAAIRAQAQVALGAGDDVAERQHALQATLAG
                     CDRATRLVEQLLMLARLETNPVRPALPVNLTAVVRRVAADLAPAALGRSQVLELEAEE
                     GCAVAADEILLGVLVRNLVDNALRYSPAGARVLVVVKKGPEGVCLCVQDSGPGMAPEA
                     IQRLGERFFRVLGTDQPGSGLGWSIVKRLVDAFGARVQIRRSEALGGLSVQVEWPT"
     gene            complement(2757..3428)
                     /locus_tag="PKX82_04565"
     CDS             complement(2757..3428)
                     /locus_tag="PKX82_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOF51554.1"
                     /translation="MRILLAEDDSMLGDGLRAGLRQLGFQVDWVRDGLAAERELGSGV
                     YAAGVLDLGLPLKDGLDVLQALRVRQIQTPVLVLTARDAIPDRVRGLDLGADDYVVKP
                     VDLHELAARLRSLVRRAHGQMQDVLTCDRVVLDPSARQVRLDGQPVTLSTREFDLLHA
                     LMRNAGRVLSREQLEQQMYSWGHEVESNTIEVHIHHLRRKLHPEAIQTVRGVGYTMLK
                     AIVSP"
     gene            complement(3482..4249)
                     /locus_tag="PKX82_04570"
     CDS             complement(3482..4249)
                     /locus_tag="PKX82_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOF51555.1"
                     /translation="MSNPSPSVSGAAPASSGATASTPKSRRIVDAPTRMFHWLFALSF
                     LGAYLTGDGERLRLLHVVLGYTMAGLLAFRVLNGLMGPKYARLSLLWRKLAGWPQWLR
                     SFAQGDATASVNWRQGQNLIMALAVAALLVLVIPLTLSGYAAYNEWGDFLGGDWVAEL
                     HEAVGKVFLMVVLGHLALIAALSVLRRKNQALPMLTGRTPGPGPDLVRNNQLGLASLI
                     LVVVLSYWTWEWQQSPSGLISVQALSDMASGREDDDD"
     gene            complement(4275..4751)
                     /locus_tag="PKX82_04575"
     CDS             complement(4275..4751)
                     /locus_tag="PKX82_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HOF51556.1"
                     /translation="MAAAWVLGAGPALADYTAPRVPPLPKYTQECAACHVAYPAGMLP
                     AASWQRMMGTLNKHYGTDASLDEASVREISTWLKANAGTYRKVREEPPQDRISQSAWF
                     VREHREVSAEVWKHKAVRSAANCAACHTRADAGDYSERNITYPAGLGEKYRHGWND"
     gene            complement(4837..5238)
                     /locus_tag="PKX82_04580"
     CDS             complement(4837..5238)
                     /locus_tag="PKX82_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HOF51557.1"
                     /translation="MHTVTSVLRALALGALVFSAQAQAVTPAEQLGGYTSKAGSPAQV
                     ARGQAFFTSKHGKDWSCASCHNAPPTTEGKHASTGKRIDTLAPAFNPKGFTDAAKTEK
                     WFRRNCNDVIGRECSASEKADVLAWLMTFKP"
     gene            complement(5307..>6730)
                     /locus_tag="PKX82_04585"
     CDS             complement(5307..>6730)
                     /locus_tag="PKX82_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOF51558.1"
                     /translation="GGAVRASIIKATQTKAAGDQEGALKELNGVLVPAVNKYMATLRE
                     MIALQAKEFEEAKLEYAKQRDDAALFARLMVAVLVLVLIGGTLLLIKQIRQPLHDAIA
                     VAETIASGDLTAKVDTTRGDEFGQMMRAIAHMQDRLVHLVADVRRGTDSMASVSEEIA
                     AGNNDLSQRTEQSAASLEETASSMQQLTATVKQSADSARQANQLAASAAQVAQRGGQV
                     VGQVVSTMDDINASSRKISDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS
                     EVRSLAGRSADAAKEIKLLINTSVEKVEGGSALVAQAGETMTEIVSSVQRVTDIMGEI
                     TAAASEQSDGIAQVNSAIGHLDQMTQQNAALVEESAAAASSMKDQVQRLAQVVATFKL
                     DGSVVSTFQTASSQPVRKPLPRPVQRSALSAPAKVSKPALAAQRPATLSKPAAAPAAK
                     PKAISAPAAKPAANKADEEWETF"
BASE COUNT         1221 a         2171 c         2053 g         1285 t
ORIGIN      
        1 ttctcggtgc ggtgttcaga ccttggatag ccagtgggcg tacttgggat tgcgaccatt
       61 gacgatgtca aagaacgctg cctggatttt ttcggtgatc gggccgcgcg aaccgctgcc
      121 gatttcaatg cggtccactt cgcggatggg ggtcacttca gcggcggttc cggtgaagaa
      181 cacttcgtcg gagatgtaca gctcgtcgcg ggtaatgcgc ttttgcacca gctccagccc
      241 caggtccttg cagatgtgca gcacggtatt gcgggtgatg ccgttgaggg cgcctgcgga
      301 caggtcgggg gtgtagacca cgccgtcctt gatcacaaaa atgttctcgc ctgcaccttc
      361 ggacacaaag ccgctggcgt cgagcaggat ggcttcgtcg taaccgtcat cggtggcttc
      421 catattggcc aggatcgagt tggtgtagtt gctgaccgcc ttggcctggg tcatggtgat
      481 gttcacatgg tggcgggtgt agctggaaat tttgacgcgg ataccgcgct tcatgccttc
      541 ttcgcccagg tacgcacccc agggccaggc ggcgaccatc aggtggatgg tgttgccctt
      601 gggagaaaca cccagcttgc ggtcacccac ccaggtcagg ggacgcaggt agcctgactc
      661 cagcttgttc tcgcgaatga cggctttttg cgcttcgttg acctgttcct tggtgaacgg
      721 gatcttcatg cgcaagatct tggcgctgtt gaacaggcgg tcggtgtgct cttccagacg
      781 gaagatggaa gtcacgccgc tatcgccctt gtacgcgcgc acgccttcga aggcaccaca
      841 accgtaatgc agggtgtggg tcagcacatg gatcttggcg tcacgccagt ccaccatctg
      901 gccatccatc cagatttttc cgtcgcggtc agacatggag ggaggaattg cgctcatcga
      961 aggtgtcctt aaagggattc agttgcaaat agtgcgaatg tccgatttta cggggccggg
     1021 cggtgtaatt cccacgcctt gagccggcca gccacgaact cgcaggttac gtgggactgc
     1081 gacgcatcgg tccagcgaaa gcgctcgggc tgggtgtctt tgggcgacag tagctcaccc
     1141 agggagcggg tcatggccac cacgtgcaag agtgtcgccc cggactgcaa tttggcattc
     1201 agcatcacgg cactttcgac atagcccaca gggcggttgg cagcacgctt gaggatttgc
     1261 agcatgcggg tgaaatgcag cagcatccac atcaccagcg cacccacggc cgctgccaca
     1321 ccggcccagc cataggcgcg gtaggcggcc accaccagca cgacgctggc aatcggaatc
     1381 aggattttct gaaattgcat gcgcccgatt ttgtcaggtc ggccactcca cctgaacaga
     1441 cagccctccc agcgcctcgg atcggcggat ctgcacacgc gcaccgaatg cgtcgaccag
     1501 acgcttcacg atggaccagc ccaggccact gccaggctgg tcggtgccca gtacgcgaaa
     1561 gaaccgctcg cccagacgct ggatggcttc gggcgccatg ccgggaccgc tgtcctgcac
     1621 acacaggcag acgccctcgg ggcccttctt caccaccacc aggacccgcg cgccggcagg
     1681 gctgtaacgc aaggcgttgt ctaccaggtt gcgcaccaga acccccagca ggatttcatc
     1741 ggcggccacc gcgcagcctt cctcggcttc cagctccagc acctgggagc gacccagggc
     1801 agccggcgcc agatcggccg ccacgcgccg gaccaccgcc gtgaggttga ccggcagtgc
     1861 aggccgcacc ggattcgtct ccaggcgtgc cagcatcagc agttgctcga ccagccgcgt
     1921 ggcccggtca cagcctgcca gcgtggcctg caaggcatgc tggcgctcag ccacatcgtc
     1981 cccggcaccc aaagcgacct gggcctgggc ccggatggcg gcaatcgggg tgcgcaactc
     2041 gtgggccgcg tctgcggtga agcgccgctc ggacaccacc atgcgcccga tgcgttccag
     2101 caactcgttg agggcaccca ccagcgggcg catttcttca ggacagtcgt ccagcaccac
     2161 aggttcgagt gtttgcggag cccgctgcgc caacgcttca ctgagtgcac gcaaggggga
     2221 aagcccggcc cggaccgacc accacaaggc cgcagccagc aagggcaacg ccagcaccag
     2281 tggcaacagc aaaccgcgca tcaccgccca gagaatgtca ttgcgtgatt cggtacgctc
     2341 ccccacaaac acctgtacat cgcgctctgc gccacgggcg ccgaataccc gccactcctt
     2401 gccatcgccg agttttcggg tgctgaagcc ggtcgtcttg tccgacatcg gctgctcgct
     2461 ggcattggtc gtgcgggtaa tcaggatgcc ctcatggaat acctgaaacg ccaccttggg
     2521 cgcatatttg tgcagcgacg gagcgtccag cacaccatcg tcatcgcctt cggattccga
     2581 ctgctgcacc accaacaggc cggccgcctg cgcaaggtgt ccgtcgagca gttcgtccag
     2641 ttcgtggctg gcatcgatcc acgtaaacag cgcggcggcc agccacacca gtgtcaccag
     2701 ccccaccacc agcgtgagca cacgcgcttg cagcgaacgc gggggttggg gcgcggtcat
     2761 ggcgacacga tcgctttgag catggtgtag cccacgcccc gcaccgtctg gatagcctcc
     2821 gggtgcagtt tgcgccgcag atggtggatg tgcacttcaa tcgtgttgct ttccacctcg
     2881 tgcccccagc tgtacatctg ctgctccagc tgttcgcgcg acagcacacg cccggcgttg
     2941 cgcatcagcg cgtgcagcaa atcaaattcg cgggtggaca aggtcaccgg ctgaccatcc
     3001 aggcgcacct ggcgcgccga agggtccagc accacgcggt cgcaggtcag cacatcctgc
     3061 atttgcccgt gggcccgccg gaccagggaa cgcaaacgcg ccgccagctc atgcaaatcg
     3121 accggcttga cgacatagtc gtcagcgccc aggtcgaggc cccgcacccg gtccggaatg
     3181 gcgtcgcgtg ctgtcagcac caggaccggg gtctggatct ggcggacacg cagggcctgc
     3241 agcacatcga ggccatcctt gagcggcagc cccagatcga gcacgcccgc cgcgtacacc
     3301 cccgaaccca gctcgcgctc ggccgccagg ccatcgcgca cccaatccac ctgaaagccc
     3361 agctggcgca gccccgcacg caggccatcg cccagcatgg aatcgtcttc ggccaaaagt
     3421 atgcgcaccg gggttgctcc gcctaaatca ctacaaacgc tgattttgta ccacgcgctg
     3481 ctcagtcatc atcgtcttcg cgtccgcttg ccatatcgct cagggcctga accgaaatca
     3541 gcccgctggg cgactgctgc cactcccagg tccagtaact gagtaccacc accagaataa
     3601 gtgaagccag gcccaattgg ttgttgcgca ccaggtcggg acccgggcct ggtgtgcggc
     3661 ccgtgagcat gggcaacgcc tggtttttgc ggcgaagaac actcaaggct gcaatcaggg
     3721 ccaaatgacc cagcaccacc atcaggaaaa ctttacccac agcttcgtgc agctcggcca
     3781 cccagtcacc acccagaaaa tcaccccatt cgttgtaggc agcgtagcca gagagcgtga
     3841 gcggaatcac cagcaccaga agtgccgcca cggccagagc catgatcagg ttctggccct
     3901 gacgccagtt gacggacgcc gtggcgtcgc cctgcgcaaa cgacctgagc cactgcggcc
     3961 agccggccaa cttgcgccac aacagcgaca ggcgcgcgta cttgggaccc atgagtccgt
     4021 tcagcacccg aaaggcaagc aaaccggcca tggtgtagcc cagcaccacg tgcaacaggc
     4081 gcaggcgttc gccgtcgccc gtgaggtaag cgcccaggaa gctcaacgca aacagccagt
     4141 ggaacatgcg tgtgggcgcg tccacgattc ggcggctctt gggggtgctg gcggtcgctc
     4201 cactggaagc tggtgctgcg ccagacactg agggcgaggg gttgctcatg ttcattgaat
     4261 tctcctgaaa tggttcagtc attccagccg tggcggtact tttcacccag cccggcgggg
     4321 taggtaatgt tgcgttcgct gtagtcacct gcgtcggccc tggtatggca ggcggcgcag
     4381 ttggcagcgc tcctgactgc cttgtgtttc cacacctcgg cgctgacctc ccggtgctcg
     4441 cgcacgaacc aggcggactg gctaatgcgg tcttgtggcg gttcctcgcg caccttgcgg
     4501 taggtgcctg cattggcctt gagccaggtg cttatttcgc gcacgctggc ttcgtcaagg
     4561 gaggcatcgg tgccgtagtg cttgttcagc gttcccatca tgcgttgcca ggaagccgca
     4621 ggcaacatgc ctgccgggta agccacgtgg caggcggcgc actcctgggt gtatttgggc
     4681 aggggtggca cgcgcggggc ggtgtagtca gccagcgcgg ggccagcgcc cagcacccag
     4741 gctgctgcca acagagaaaa tgctattgtt tttatagcaa catgcttata tttcacgggg
     4801 gccagaggca tatttttcat agatattctc gcgttttcag ggtttgaagg tcatgagcca
     4861 ggccagcaca tcggctttct cgctggcgct gcattcgcgg ccgatcacgt cattgcagtt
     4921 gcgacgaaac catttctcgg tcttggccgc atcggtaaag cccttggggt tgaaggccgg
     4981 tgccagggtg tcaatacgct tgccggtgct ggcgtgcttg ccttctgtcg tgggcggcgc
     5041 gttgtggcag gaggcgcagc tccagtcctt gccgtgtttg ctggtgaaaa acgcctggcc
     5101 ccgcgccacc tgtgcggggc tgccggcttt ggacgtgtag ccccccagtt gctctgctgg
     5161 cgtaacggcc tgggcctggg ccgaaaagac gagtgcgccc aaggccaggg cgcgtagtac
     5221 tgatgtcact gtgtgcataa aacctccatg cacgcatggt aggcagcaca cattaagcgc
     5281 gccttaaggg cgcgccggag ccaggatcag aaggtttccc actcttcgtc cgccttgttg
     5341 gccgctggct tggcagcggg tgcactgatg gctttgggct tggctgcggg tgctgctgca
     5401 ggtttcgaca gtgtcgcagg acgctgggcg gcaagcgccg gtttgctgac cttggcaggg
     5461 gccgacaacg cactgcgttg caccggcctc ggcaggggtt tgcgcactgg ctggctcgac
     5521 gcagtctgga aggtggagac cacgctgcca tccagcttga aagtagccac cacctgggcc
     5581 aagcgctgga cctggtcttt catgctgctg gccgctgcgg ccgactcttc caccaatgcc
     5641 gcgttctgct gcgtcatctg gtccaggtga ccaatcgcac tgttgacctg ggcaatacca
     5701 tcggactgct cggaagcggc ggctgtaatc tcacccatga tgtcggtcac acgctgcacg
     5761 gacgacacga tctcggtcat ggtttcaccc gcttgtgcca ccaatgcgga tccgccttcg
     5821 accttctcga cgctggtatt gatcagcagc ttgatttcct tggccgcatc ggccgagcgt
     5881 ccggccaggc tacgcacctc actggccacc acagcgaagc cccggccctg ttcgcctgcg
     5941 cgggcagcct ccacggcagc gttcaacgcc agaatattgg tctggaacgc gataccgtcg
     6001 atcacgctga tgatgtcgga aattttgcgt gaactggcgt tgatgtcgtc catggtgctg
     6061 accacctgac ccaccacctg accaccgcgc tgcgccacct gggcggcaga agcggccagc
     6121 tggttcgcct ggcgtgccga gtcggcagac tgtttgacgg tggcagtgag ctgctgcatg
     6181 ctggacgccg tttcttccag gctggccgcc gactgctcgg tgcgctggga caggtcgttg
     6241 ttgcccgcgg cgatctcttc gctcacactg gccatgctgt cggtaccccg acgtacatcc
     6301 gcgaccagat ggaccagccg gtcctgcata tgggcgatgg cgcgcatcat ctggccgaat
     6361 tcgtcgccgc gcgtggtgtc gactttcgct gtcaggtcac ccgaggcaat ggtctcggcc
     6421 accgcgatgg catcgtgcag gggctggcgg atctgcttga tcaggagcag cgtgccacca
     6481 atcaaaacca gtaccagcac cgcaaccatc agccgggcaa ataaggccgc gtcgtcccgc
     6541 tgtttcgcat actccagctt ggcctcttcg aattccttgg cttgcaacgc aatcatttcg
     6601 cgcaaagtgg ccatgtactt gttgaccgcc gggaccagca ccccattcaa ctccttgagt
     6661 gcaccttcct ggtcacccgc agctttggtc tgtgtggcct tgatgatcga tgcccgaacg
     6721 gcgccaccct
//
LOCUS       DAOSJM010000090         6702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10363_length_6702_cov_4.736272, whole genome shotgun sequence.
ACCESSION   DAOSJM010000090 DAOSJM010000000
VERSION     DAOSJM010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6702
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10363_length_6702_cov_4.736272"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKX82_04590"
     CDS             <1..466
                     /locus_tag="PKX82_04590"
                     /inference="COORDINATES: protein motif:HMM:NF006518.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOF51559.1"
                     /translation="SGLLFATWLLLNESATTGNTVLGALFAFAIPLLTRPFMPDPPRV
                     KNWGTVIRLAAVVLRDIVVSNLDVAKRVLGPEAAIASRFVWIPLRIRDPHGIVALAGI
                     ITLTPGTLSSDLSPDRKHLLVHVLHCPNDTAQAALIADIQARYETPLMEIFE"
     gene            463..732
                     /locus_tag="PKX82_04595"
     CDS             463..732
                     /locus_tag="PKX82_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HOF51560.1"
                     /translation="MIDTVLPWVTGGFALALLLNVWRLLRGPGMADRVLALDTLYINA
                     LALLVCLGMWWRTTVYFEVALLIGLLGFVGTVVLSKFILRGDIIE"
     gene            729..1043
                     /locus_tag="PKX82_04600"
     CDS             729..1043
                     /locus_tag="PKX82_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009871327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HOF51561.1"
                     /translation="MNTLLEWAVVALLVIGVLFALIGSWGLAKFGDFFKRLHGPSKAT
                     TLGVGCVLLASMLWLAGQGRASGHELLITLFLFLTAPVSAHLLVKAAMKLTPGSRPPP
                     PP"
     gene            complement(1151..2110)
                     /locus_tag="PKX82_04605"
     CDS             complement(1151..2110)
                     /locus_tag="PKX82_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOF51562.1"
                     /translation="MQTVAPLWLWATFIGIVLVSLFVDFVVLKKQGAHDIGVKEALNW
                     SLIWIALSFLFNGLFWWAVKDSTGSTEMANTKSLEFLTGYLIEKSLAVDNIFVFLLIF
                     TYFAVPTHFQKRVLMIGIIGAIVLRTIMILVGGWLLSEFHWILYVFGAFLILTGVKMW
                     WAAGKESDLEDNPALKLLRKVLPVSKNYDGENFWTVENGKKIATPLFMVICLIALTDV
                     IFAVDSIPAIFAITTDPFVVLTSNVFAILGLRAMYFLLAAVANKFHLLNYGLAVILVF
                     IGTKMCLIDIYKIPVGISLGVVVGILALTMLLSLRSATTQPRG"
     gene            2382..2906
                     /locus_tag="PKX82_04610"
     CDS             2382..2906
                     /locus_tag="PKX82_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HOF51563.1"
                     /translation="MKRTKSKWKERINKSLPNRDGLSAHPWLQPVAKRLLHPQLWRFQ
                     HEAVARGVAVGTFWAFVIPFAQIVVAAAHCTWWRANIPAAAAMTMVTNPLTIGFWLWL
                     AYQAGALVLGQPLVQPPSVGGGAPAWLEEFGWPTVLGMGMFAVGGASMGYIAVKLVWR
                     LRVSMKLRTRRAGT"
     gene            3035..3967
                     /locus_tag="PKX82_04615"
     CDS             3035..3967
                     /locus_tag="PKX82_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HOF51564.1"
                     /translation="MKRLLSLLTLVFAVGLSSVAMEAEAAKRMGSGKSMGTQRQATPD
                     KAPTAAPAAAGPAAGAAAAAPSRSWMGPVAGLAAGLGIAALASHLGFGDELASMVMMG
                     LIAAAIMVAIGFFMRKRAAAQKSSATAPGGLQYAHVNPATARNASSGADNNTPAYKVL
                     MPANGGSTIGSGIGSGIGANSTNASRIPADFDTAGFERNAKVNFIRLQAANDAGNLDD
                     IRQFTTPEMFAELKMELADRGAATQKTDVVSINASVIEVDEDADRYLVSVRFTGVIRD
                     DSNEPDEAFNEIWHLMKSRQGNSGWVLSGIQQMS"
     gene            3964..4323
                     /locus_tag="PKX82_04620"
     CDS             3964..4323
                     /locus_tag="PKX82_04620"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HOF51565.1"
                     /translation="MILADYLPVLLFIAVGIAVGGVPQVLGYVLGPNRPDKEKNSAYE
                     CGFEAFGDARMPFDVRYYLVAILFILFDLEIAFLFPWAVSLHEIAPTGFWAGMVFLGI
                     LAVGFIYEWKRGALNWE"
     gene            4355..4825
                     /locus_tag="PKX82_04625"
     CDS             4355..4825
                     /locus_tag="PKX82_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOF51566.1"
                     /translation="MIDVVKLMHLVAAIFWMGGMAFMLLALRPAAVALLEPPERLMLM
                     GAVWKRFFLIVMVSIVALFATGTHLYTAAFRAIKAATGQGAVPLGWNVMLVLGLLMMA
                     IFGHIYFAGYAKFKRAMAVQEWPLAGKAAAQIHTLMVTNFVLGWLAIAAVRLVR"
     gene            4837..5034
                     /locus_tag="PKX82_04630"
     CDS             4837..5034
                     /locus_tag="PKX82_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51567.1"
                     /translation="MKVTRLYQPRNPLFWIMLVLNLLSAVLAWLTRAYSLDALGSVLV
                     TAFAVGNALLGTYLAWRLMQS"
     gene            5066..6193
                     /gene="hemE"
                     /locus_tag="PKX82_04635"
     CDS             5066..6193
                     /gene="hemE"
                     /locus_tag="PKX82_04635"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOF51568.1"
                     /translation="MTDTTFDTLHNPTFLQACMRQATPHTPMWLMRQAGRYLPEYVAT
                     RAKAGSFMGLATNTQYATEVTLQPLDRYPLDAAILFSDILTVPDAMGLGLSFAQGEGP
                     RFASPVKTEADVAKLAVPDMAKLQYVFDAVSSIRKALTQDGKGRVPLIGFSGSPWTLA
                     CYMVEGKGSDDYRLVKTMLYSRPDLMHKMLAVNADAVAAYLNAQIDAGAQAVMIFDSW
                     GGVLADGAFQSFSLAYTARVLAQLKRHGTDGKIVPRIVFTKGGGLWLQDMKALNCEVL
                     GLDWTVNLASARALVGGTENGPGKALQGNIDPNVLFAPPAAIALEVAKVLDSFGKPYQ
                     GTGTGPTHIFNLGHGISQYTPPENVAALVEAVHSHSRKLRA"
     gene            6544..>6702
                     /locus_tag="PKX82_04640"
     CDS             6544..>6702
                     /locus_tag="PKX82_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51569.1"
                     /translation="MTHWLSVLVHTPAHAALGPVLTYQSAQALQPGTLVRVPLGSRET
                     LGVVWNSDT"
BASE COUNT         1387 a         1927 c         1921 g         1467 t
ORIGIN      
        1 gtcgggcttg ttgtttgcca cctggctact gctcaacgag tcggccacca ctggcaacac
       61 ggtgctgggg gccttgtttg cctttgccat tcccttgttg acaaggccat tcatgcctga
      121 cccaccgcgc gttaaaaact ggggcacggt catccgcctg gcagcggtgg tattgcgcga
      181 tattgtggtc tccaacctgg atgtagcaaa gcgtgtgttg ggccccgaag cagctattgc
      241 gtcacggttt gtgtggatcc ccctgcgcat tcgcgacccc cacggcattg tggccctggc
      301 cggcatcatc accttgacac cgggcacgct gagcagcgac ctcagccccg accggaagca
      361 ccttctggtg catgtactgc attgccccaa cgataccgcg caagccgcgc tgattgcgga
      421 tatccaggca cgttatgaaa ccccgctgat ggagattttt gagtgatcga caccgttctt
      481 ccctgggtga ctggtggttt tgcactggcg ctgctgctca atgtctggcg gcttttgcgg
      541 ggtccgggca tggcagaccg ggtgctggcg cttgacacgc tgtacatcaa tgcactggcc
      601 ttgctggtat gccttggcat gtggtggcgc acgacggtgt actttgaagt ggcgctactg
      661 atcggacttt tgggctttgt cggcacggtg gtgctgagca aattcatttt gcggggagac
      721 atcatcgaat gaacacccta ctggaatggg ccgtggtggc cttgctggtc atcggcgtcc
      781 tgtttgcact gattggcagc tggggcctgg cgaagtttgg cgatttcttc aaacgactgc
      841 atggccccag caaggccacc actctggggg tgggttgcgt gctgcttgcc tccatgctct
      901 ggctggccgg acaggggcgc gccagcgggc acgagttgct catcaccctg tttctgtttc
      961 tcaccgctcc ggtttcggcc cacctgctgg tcaaggcagc gatgaagctc acgccgggaa
     1021 gccgcccgcc gccgccgccc tgaatcatgc gcagtttgct acttatttaa tagctaattg
     1081 cgcagtattt acggggggcc agatgccaat ttcccttgga aatcgattgg cggcagctgg
     1141 caatcgcggg ttagcccctt ggttgtgtgg tggctgagcg caggctcaac agcatggtca
     1201 gggccagaat ccccacaacc actcccagag agatgccaac cgggatcttg tagatatcga
     1261 tcaggcacat cttggttccg atgaacacca gaatcactgc cagaccgtag ttcagcaagt
     1321 gaaacttgtt ggccacagcg gcgagcagga agtacatggc gcgcaaaccc agaatggcaa
     1381 acacattact ggtcagcacg acaaaggggt ccgtggtgat ggcgaagatg gcggggatgg
     1441 aatccaccgc aaagatcaca tcggtaagcg caatcaggca gatcaccatg aacagcggtg
     1501 tagcaatctt cttgccattc tccaccgtcc agaagttctc accgtcgtag tttttgctga
     1561 ctggcaaaac cttgcgcaga agtttcaaag ccggattgtc ttccaggtca gactccttgc
     1621 ctgcggccca ccacatcttc acaccggtga ggatcaggaa cgcaccgaat acatacaaaa
     1681 tccagtggaa ctcggacagc aaccagccgc ccaccaaaat catgatggtg cgcagaacaa
     1741 tagcgccgat gatgccgatc atcagcaccc gcttctggaa gtgggtgggt accgcgaagt
     1801 aggtgaagat cagcagaaac acgaatatgt tgtccaccgc cagcgacttc tcgatcagat
     1861 agccggtcag gaattccagc gacttggtgt tggccatttc tgtggatcct gtggaatctt
     1921 ttacggccca ccagaacagg ccgttgaaca gaaagctcaa ggcaatccag atcagggacc
     1981 agttcagcgc ctctttgaca ccaatatcgt gcgcgccctg ctttttgaga acgacaaagt
     2041 caacgaacag cgacaccagc acgatgccga tgaaggtggc ccacagccac aggggagcaa
     2101 cggtttgcat attcatacct tacaagttga agcgacttcg cagtcgctga atacgttgca
     2161 ggggctaacc taagcccgct gaatgcatcg tattaaaact gaaattttct tcagcacagt
     2221 tcgaaaaaac cagagtcttc tggcgcattt ccctgctgtc agagccaccc aagggccagc
     2281 atcatccccc catcgttcag taaagggatt tacgtgttgt acgcaatttc atggttttgg
     2341 gttctggcac ttcttgccat cgcgcgcagg agagcagccc catgaagcgc accaagtcaa
     2401 aatggaagga gcggatcaac aagtcgctgc ccaaccgcga cggtttgtcg gcgcacccct
     2461 ggctgcagcc cgtggcgaaa cggctgctcc atccgcagct ttggaggttt caacacgagg
     2521 cggtggcgcg cggtgtggcg gtaggcactt tttgggcctt cgtcattcct ttcgcccaga
     2581 tcgtggtggc cgcagcacat tgcacctggt ggcgcgccaa catacctgcg gcggcggcca
     2641 tgaccatggt gaccaatccg ttaacgattg gattctggct ttggctggct taccaggcag
     2701 gcgcactggt tctgggccag cctttggtac aaccaccctc cgttggcggt ggcgcacccg
     2761 catggcttga agagtttggg tggcccacgg tgttgggcat gggcatgttt gcggtgggtg
     2821 gcgcttcaat ggggtatatc gcagtcaagc tggtttggcg tctgcgcgtc tcgatgaagc
     2881 tgcgcacgcg tcgcgcgggc acataaacaa gtgcgataag tgcggccatg acaatcctgt
     2941 tttttcgaag tttatttccg caatacgctg cttttctatt cactttgaac tgtctattct
     3001 tgacttcgtt gtttcatatt tttaggagat tcacatgaag cgcttacttt cactattgac
     3061 cctggttttt gccgtgggtc tttcctctgt ggcgatggaa gccgaggctg ccaaacgcat
     3121 gggctctggt aaatccatgg gcacacaacg tcaagccaca cccgacaagg cacccaccgc
     3181 agcgcctgct gcagccggac ccgctgcagg tgcggctgcc gccgcacctt cccgctcatg
     3241 gatgggcccg gtggccggac ttgccgcagg actgggtatc gcggctctgg cctcccatct
     3301 gggatttggc gatgagctgg catcgatggt gatgatgggc ttgatcgctg cagccatcat
     3361 ggtggccatt ggctttttca tgcgcaagcg tgcagctgcg caaaagtcca gtgcgacggc
     3421 acccggtggg ctgcaatatg cgcatgtgaa ccctgcgacc gctcgcaatg ccagcagcgg
     3481 agcggataac aatacgccag cctacaaagt cttgatgcct gccaatggcg gcagcacgat
     3541 tggctcggga attggttctg gcattggcgc aaacagcaca aacgcaagcc ggattcccgc
     3601 agacttcgat accgcaggct tcgagcgcaa cgccaaggtc aacttcatcc gtttgcaagc
     3661 cgccaacgat gccggcaacc tggacgatat ccgccagttc actacgccag aaatgtttgc
     3721 cgagctcaaa atggaattgg ctgatcgtgg agcagcaacc cagaaaaccg acgtggtgag
     3781 catcaacgcc agcgtgattg aagtggatga ggacgcagac cgctatctgg tcagcgtacg
     3841 tttcaccggc gtgatccgcg acgacagcaa cgaacccgac gaagcgttca atgaaatctg
     3901 gcacctgatg aagtcacgcc agggcaacag tggatgggta ttgtctggaa ttcagcaaat
     3961 gtcgtgattc tggctgacta cctgccggta ctgctcttca tagcagtcgg cattgcggtc
     4021 ggtggggttc cccaggtact gggctatgta ctggggccca accgacccga caaggaaaaa
     4081 aattccgcgt atgaatgcgg atttgaagca tttggcgatg cacgcatgcc gtttgacgtg
     4141 cgctactacc tggttgccat tctgttcatc ttgtttgatc tggaaatagc gtttcttttc
     4201 ccgtgggccg tgtctctcca cgagattgct cccacaggtt tttgggcagg aatggtcttt
     4261 ctaggcatac tagccgttgg ttttatatac gaatggaaaa gaggcgcgtt gaactgggag
     4321 taacacaccg ttcgaccttc ttcaatagaa aaacatgatt gatgttgtca aactgatgca
     4381 cttggtggcg gccatcttct ggatgggcgg aatggcattc atgctgctgg ccttgcggcc
     4441 tgcggccgtt gcattgctgg agccgcccga gcggctgatg ttaatggggg ccgtgtggaa
     4501 acgcttcttc ctgatcgtca tggtctccat cgtcgccctg tttgccaccg gaacgcacct
     4561 gtacaccgcg gcctttcggg ccatcaaagc ggccaccgga cagggcgccg tgccgctggg
     4621 gtggaatgtg atgctggtgt tgggcctgct gatgatggcc atctttgggc acatctactt
     4681 tgcgggttac gcaaaattca aacgtgccat ggccgttcag gagtggcctc tggccggcaa
     4741 ggcggctgca caaatccaca cgctgatggt caccaacttc gttttgggct ggctggccat
     4801 cgccgcagtg cgtctggtgc gctgacgcac cctcccatga aagtcacgcg cctctatcag
     4861 ccgcgcaatc cacttttctg gatcatgctg gtgctcaatc tgctgtccgc cgttttggcg
     4921 tggctgacgc gcgcatattc cctggatgcc ctgggcagcg tgctggtaac cgcctttgca
     4981 gtaggcaatg ccctgctggg cacctatctg gcctggcgac tgatgcagtc ctgagcccgc
     5041 aaagggaact gcgacaatat tgggtatgac cgataccacc ttcgacaccc tgcacaaccc
     5101 cacatttctg caagcctgca tgcgccaggc caccccgcac acgcccatgt ggctgatgcg
     5161 ccaggcgggc cgttacctgc ctgaatatgt ggccacccgc gccaaggccg gcagctttat
     5221 gggcctggcc accaacaccc agtacgccac cgaagtcacc ctgcaaccgc tggaccgtta
     5281 tccgctggac gcggctattc tattttccga catcctgacc gtgcccgacg cgatgggcct
     5341 ggggctgtcg tttgcgcagg gcgaaggccc gcgctttgcc agccccgtca agaccgaagc
     5401 cgacgtcgcc aaactcgcag tgcccgacat ggccaagctg cagtacgtgt ttgacgcggt
     5461 atccagcatc cgcaaggcgc tcacccagga cggcaagggc cgcgtaccgc tgattggttt
     5521 tagcggcagc ccctggacgc tggcctgcta catggtcgaa ggcaagggca gcgacgacta
     5581 ccgcctggtc aaaaccatgc tgtacagccg cccggacctg atgcacaaga tgctggcggt
     5641 gaatgccgat gccgtggccg cttacctgaa cgcgcagatt gatgccggcg cccaggcagt
     5701 gatgattttt gacagctggg gcggcgtgct ggccgacggt gcgttccaga gtttcagcct
     5761 ggcctacacc gcgcgcgtgc tcgcgcagct caagcgccat ggcacggacg gcaagatcgt
     5821 gccgcgcatt gtgttcacca agggcggcgg actgtggttg caggatatga aggcgctgaa
     5881 ctgcgaagtg ctgggcctgg actggaccgt gaatctggcc agcgcgcgcg ctttggtggg
     5941 cggaactgag aacggccctg gcaaggcttt gcaaggcaat atcgacccca acgtcctgtt
     6001 tgcgccaccg gctgctattg cattggaagt ggccaaggta ctcgacagct tcggcaaacc
     6061 ctaccagggc acaggaaccg gccccacgca catcttcaat ctgggccacg gcatcagtca
     6121 atacacccca ccggagaacg tggcggcgct ggtcgaagcc gtgcacagcc attcgcgcaa
     6181 attgcgcgcg taatcccata gcatcttttt tagcggtttt ttgattgctg cactgcactt
     6241 atgcacaaaa aagtgctagc gggggcaaac tgctataagt ttcgaagcgc aaccgctaca
     6301 aactcgatag cgcacgtaag ttgttgattc ataaggaatt taaactttgc tttttttctg
     6361 ggcattccag ccgaagcctt gattttcaag gcttcggaag gactcagggc aagatataaa
     6421 caaagttatc cacagaaaca ctgaactaag aacaaagtct ttacaaatca agcacttagc
     6481 ctttgtttct cctaaataca tcaacaagcc accccaagtg aaccctacgc gcacgtcaac
     6541 cccatgaccc attggctgag cgtgcttgtc cacacccccg cgcatgctgc gctggggcca
     6601 gtgctgacct accagagtgc gcaggcgctg cagcccggca ccctggtacg cgtgccgctg
     6661 ggctcgcgcg aaaccctggg tgtggtgtgg aacagcgaca cc
//
LOCUS       DAOSJM010000091         6682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10398_length_6682_cov_4.423419, whole genome shotgun sequence.
ACCESSION   DAOSJM010000091 DAOSJM010000000
VERSION     DAOSJM010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10398_length_6682_cov_4.423419"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="PKX82_04645"
     CDS             complement(<1..969)
                     /locus_tag="PKX82_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOF51570.1"
                     /translation="MKHPNNPIRLLSEILGIVAVCEVVVMVSMPRLASGLSPLAAGLL
                     DVALLVLLAGPAVYWRSMAITRNAAAAARAPGPSIRSAVILTVTAQLLGLVLTVVGVW
                     WQQRDIEVAGRVRFDRAVDRVEAEVRRRLEFPVFGLTGIRAAYAASDGVTRNEFRNLV
                     QTLNIEQQFPGVRGFGYVERIARADANAYAKRQQAEGMPEFHIQSLGRSDDLYVVKHI
                     EPLESNYEALGIDLGQDLVRREALERAARSGDASLSGRITLLRDGVFNTGFIYVLPVY
                     RKGTDPVTDRQRIAALAGLLSAPMVASEVMGGVADVAMEAVQVELFD"
     gene            complement(1060..2310)
                     /locus_tag="PKX82_04650"
     CDS             complement(1060..2310)
                     /locus_tag="PKX82_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOF51571.1"
                     /translation="MTTKPSALGHLKVLDLSRVLAGPWCTQMLADLGADVIKVERPGA
                     GDDTRHWGPPFLQDADGNDTTQATYFTACNRNKRSITIDMAQPEGQALIRQMAAQSDI
                     LVENFKVGGLKAYGLDYESLKALNPRLIYCSVTGFGQDGPYAERAGYDLMIQAMTGMM
                     SITGRADDVPGGGPLRVGVALTDLFTGCYATSAILAALEVRNRTGAGQHIDMALLDVG
                     MAILANQAAGFLNTGKVPQRQGNSHPSLAPYQDFPTQDGSMLLAIGNDGQFARFCEAA
                     GKPEWAADPRYASNTLRVKNRETLIPAMEAVTRTRTTADWVALLEDKAVPCGPINHIG
                     QAFDDAQVLARGLKVNQPVAPVVYAQSAIKTIATVASPLRLTATPPVLRNAPPMLGEH
                     TDEVLAGLGLDGAAIARLKSAGVV"
     gene            complement(2645..3841)
                     /locus_tag="PKX82_04655"
     CDS             complement(2645..3841)
                     /locus_tag="PKX82_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOF51572.1"
                     /translation="MAHAAFNWQDPFLLDSQLTDEERMVKEAAAAYCQDKLLPRVTMA
                     FREGTTDPAIFREMGELGLLGPTIPEQYGGPGLNYVSYGLIAREVERVDSGYRSMMSV
                     QSSLVMVPIFEFGTEAQRQKYLPKLATGEWIGCFGLTEPDHGSDPGSMATRAHKVAGG
                     YKLTGSKMWISNSPIADVFVVWAKEVSESGQVGPIRGFVLEKGFKGLSAPAIHSKVGL
                     RASITGEIVMDGVFCPEENAFPEVQGLKGPFTCLNSARYGIAWGALGAAEDCWTRARQ
                     YVLDRKQFGRPLAANQLIQKKLADMQTEIALGLQGCLRLGRMKDEGTASVEITSILKR
                     NSCGKSLDIARMARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDIHALILGRAMTG
                     IAAFGN"
     gene            3949..4875
                     /locus_tag="PKX82_04660"
     CDS             3949..4875
                     /locus_tag="PKX82_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOF51573.1"
                     /translation="MRRKIPSLQALACFDAAARHESYTRAAQELALTQSAVSRQIGAL
                     EDYLGLPLFRRTRHGVVLTTAGSQYARQIAPRLQALERDTLDAMSGQGAGGAVNLATV
                     PTFAARWLIPRLPDFAKHHPDVVIHMDTRTRPFMFAETEFDAALYASTPDAIGQWAGT
                     HALPLLPEVVLPVCAPALLGGRTTVTAAELATLPLLQQSTRPYAWPQWLEAAGVGGEV
                     DDADSAPASVLAGPRYEQFSMAAAVAVHGLGVGLIPRLLVEAELASGALVVAHPQTLP
                     GERFYYLVQPEGTQVRPALQRFIDWLQQASAG"
     gene            complement(4894..5907)
                     /locus_tag="PKX82_04665"
     CDS             complement(4894..5907)
                     /locus_tag="PKX82_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51574.1"
                     /translation="MSIRLLTAAVLALGLSACATGPGAQHGTYSFGVWGDMPYKKAGD
                     ESKLPAVLQSINQSNIAFSIYDGDIKDGSSKCTDDIYTDAVKMFGTLVKPVVYVPGDN
                     EWTDCHRTNNGGFDGLERLKHLRKIMFPTANSLGQTTMPLLHQGKLGEKYVENTRFSH
                     GPVVFAGLNVPGSNNNVVLSAKECTNKSARTPAQCDASNAEYLERDAANVVWLEQTFQ
                     QAKDTGAQGVVLVIQADPGFDLPETEEVDESQQPRFNGYRNFVAQVIKQTEGFKGQVL
                     LVHGDTHFFKVDKPLYSPAKLLPNLTRLQTFGSPSLHWVKVTVDAGSDQLFQIQPVIV
                     RQP"
     gene            6093..>6682
                     /locus_tag="PKX82_04670"
     CDS             6093..>6682
                     /locus_tag="PKX82_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51575.1"
                     /translation="MSVSTTPQPDVRPGRLAASEYALRFADASPRLTHSQALLEAERC
                     LYCFDAPCATACPTSIDVPSFIKRIADENLRGAARTILESNPLGGMCARVCPTENLCE
                     AVCVRNTQEGVPVAIGRLQRHAVDALLESAKPQLFTRAAPTGKKVAVVGAGPAGLACA
                     HTLARMGHDVVVFDARPKAGGLNEYGLASYKTPDNFA"
BASE COUNT         1196 a         2119 c         2099 g         1268 t
ORIGIN      
        1 atcaaacaac tccacctgaa ccgcttccat ggccacatcg gccacaccgc ccatcacttc
       61 cgatgccacc atcggtgcag acagaagtcc ggcgagcgcg gcgatgcgct gcctgtctgt
      121 caccggatca gtccccttgc ggtacaccgg aaggacatag atgaagccgg tgttgaatac
      181 gccatcgcgc aaaagagtaa tgcggcctga cagggacgcg tcccccgaac gggccgcgcg
      241 ctcaagtgcc tccctgcgca caagatcctg ccccagatcg atgcccaaag cctcgtaatt
      301 ggattccaga ggttcaatgt gtttgaccac atagaggtca tccgaacgtc ccagactttg
      361 gatgtggaat tccggcatgc cttccgcctg ctggcgcttg gcataggcat ttgcgtcagc
      421 gcgcgcaatc cgctcgacgt agccgaagcc ccgcacgcca ggaaattgct gttcgatgtt
      481 cagtgtttgc accaaattgc ggaattcgtt gcgggtcacc ccatcgctgg ctgcgtaggc
      541 tgcgcggata ccggtgagcc cgaataccgg aaactccagc ctgcggcgta cttcagcctc
      601 cacccggtcc accgcccggt cgaaccgaac ccgacctgcc acctcaatat cacgctgctg
      661 ccaccacaca cccacaaccg tcagtaccag accgagtaac tgggccgtca ctgtcaggat
      721 aacggcagac cggatgctcg gccccggggc gcgagcggcc gccgctgcgt tcctggtgat
      781 ggccatgctg cgccagtaca ccgcaggacc tgccagcaac accagcaatg cgacatccag
      841 taatcccgcg gccagcgggc tcaggccaga cgccagccgg ggcatggaga ccatcaccac
      901 cacctcgcac actgccacga tgccgagaat ttcggacaga aggcgtatcg ggttattggg
      961 gtgtttcatg ctgcgtaaag acgtgccata gcgccgcaat cattgcagcc ggtggtcaga
     1021 atatcagaac accccagcct tgccgagccc ctgcgcgggc tagaccacgc cagcactttt
     1081 caggcgtgca atcgctgcgc catccagtcc cagcccggcc agcacctcgt cggtgtgctc
     1141 gcccagcatc gggggcgcat tgcgcagcac cggcggggtg gcagtcagcc gcaacgggct
     1201 ggccacggtt gctattgttt taatagctga ttgtgcatat accacggggg ctacaggctg
     1261 atttaccttc aaaccccggg ccagaacctg ggcatcatca aaagcctgac cgatatggtt
     1321 gatggggcca cagggcacgg ccttgtcttc cagcagcgcc acccaatcgg ccgtggtgcg
     1381 ggtgcgggtc acagcttcca tggccgggat cagcgtctcg cggtttttta cacgcagggt
     1441 attgctggcg tagcgcgggt ccgctgccca ctcgggcttg cccgcagctt cgcagaagcg
     1501 cgcgaactgg ccatcgttgc caatggccag cagcatggag ccgtcctgcg tggggaagtc
     1561 ctggtacggt gccaggctgg ggtggctgtt gccctggcgc tgcggcacct tgcccgtgtt
     1621 caaaaaccca gcagcctggt tagccagaat cgccatgccc acatcgagca aggccatgtc
     1681 gatgtgctgg cccgcaccgg tgcggttgcg cacttccaga gctgccaaga tggcgctggt
     1741 ggcgtagcag ccggtgaaca ggtcggtcag cgccacgccc acgcgcagcg ggccaccgcc
     1801 gggcacgtcg tcggcacggc cggtgatgct catcatgccg gtcatggcct ggatcatcag
     1861 gtcgtagccc gcgcgctcgg catacgggcc gtcctggccg aagccggtga ccgagcagta
     1921 gatcagccgg gggttgagcg ccttgaggct ctcatagtcc agcccatacg ccttgagccc
     1981 acccactttg aagttctcca ccaggatgtc gctttgcgca gccatctggc ggatcagcgc
     2041 ctggccttcg ggctgggcca tgtcgatggt gatggagcgc ttgttgcggt tgcaggcggt
     2101 gaaataagtg gcctgcgtgg tgtcgttacc gtccgcgtcc tggaggaacg gggggcccca
     2161 gtggcgggtg tcgtcgcccg cgcccgggcg ctcgaccttg atcacatcgg cgcccaggtc
     2221 ggcgagcatc tgggtgcacc aggggccggc caggactctg gacaagtcga ggactttgag
     2281 gtggccgagg gccgagggtt ttgttgtcat gtctgtaggt ttaataggtg ttgccaacgg
     2341 tgttgtttgc gcctgcgtcc tggcctgccg tgcggagtat ggccggggcg ctcatactgc
     2401 caaaagcgca gaaatcgcgc cccagccata cccctcccgg cgagagacgc atggtggtct
     2461 gtgaatggta aatacatgcc ggagatactg cgcgtgcacg ttcacgggct cacccaagtg
     2521 caccgaatgc gcgaagcggg gtaccgggac tcgccgattt ctggccagca ggacagtatg
     2581 aggcggggcc cggtaccccg cttggcgcta cccaccgtgc tggcaagcga gggcccaaga
     2641 aacatcagtt cccgaacgcc gcaatccccg tcatcgccct gcccagaatc agggcgtgga
     2701 tgtcgtgcgt gccttcgtag gtattcacca cttccagatt caccagatgc cgcgcgactc
     2761 cgaactcgtc gctgatgccg ttgccgccca tcatgtcgcg cgccatacgg gcgatgtcca
     2821 gcgacttgcc gcagctgttg cgcttgagga tggaagtgat ttccaccgac gccgtgcctt
     2881 cgtctttcat gcggcccaga cgcaggcagc cttgcaggcc cagtgcgatt tcggtctgca
     2941 tgtcggccag cttcttctgg atcagctggt tggcggccag ggggcggccg aactgcttgc
     3001 ggtccagcac atactggcgg gcacgcgtcc agcagtcttc ggccgcaccc aaagcacccc
     3061 aggcaatgcc gtagcgggcg ctgttgaggc aggtgaatgg gcctttgagg ccttggactt
     3121 cggggaaggc gttttcttca gggcagaaca cgccgtccat cacgatctcg ccggtgatgc
     3181 tggcgcgcag gcccaccttg ctgtggatgg cgggggcgct caaacccttg aagccttttt
     3241 ccagcacaaa accgcggatc ggaccgacct gaccagactc gctgacctcc ttggcccaga
     3301 ccacaaacac gtcggcaatc gggctgttgc tgatccacat cttggagccg gtgagcttgt
     3361 agccgcccgc caccttgtgg gcgcgggtgg ccatgctgcc ggggtcggag ccgtggtcgg
     3421 gttcggtcag gccgaagcag ccaatccact cgccagtggc cagcttgggc aggtatttct
     3481 ggcgctgtgc ttcggtgcca aattcaaaga tcggcaccat gacaagggag ctttgcacgc
     3541 tcatcatgga gcggtaaccg gaatcgacgc gctcgacttc gcgggcgatc aggccgtagg
     3601 agacatagtt caggccgggg ccaccgtact gctcggggat cgtggggccc agcaggccga
     3661 gttcgcccat ttcgcgaaag atcgcagggt cggtggtgcc ttcgcggaac gccatcgtca
     3721 cgcggggcag cagcttgtcc tggcagtagg ccgccgccgc ttccttgacc atgcgctctt
     3781 cgtcggtgag ctgggagtcg agcagaaagg ggtcttgcca gttgaatgct gcgtgggcca
     3841 tggtgggttc cttcaatgtt ttggaatgaa tggcccgatg ttagcggccc cgtccttgag
     3901 aatcaaacgc cttgttggca tccaggtatg agtaactaga atgtctggat gcgcagaaaa
     3961 atcccctccc tgcaggcgct ggcctgtttt gacgccgccg cgcgccacga aagttatacc
     4021 cgcgccgcac aagagctggc actgacccag agcgcggtgt cacgccagat cggtgcgctg
     4081 gaggactatc tgggcttgcc cctgttccgg cgcacacgcc acggcgtggt gctgaccacc
     4141 gcaggcagcc agtacgcacg ccagatcgca ccccggctgc aggcgctgga gcgtgacaca
     4201 ctggacgcca tgtccggcca gggcgccggt ggtgccgtca atctggccac cgtgcccacc
     4261 tttgcagccc gctggctgat tccgcgcctg ccggactttg cgaagcatca cccagatgtg
     4321 gtgattcaca tggacacccg gacccggccg ttcatgtttg ccgagaccga atttgatgca
     4381 gcgctgtacg ccagcacccc ggacgcgatc gggcagtggg ccggcaccca tgcattgccc
     4441 ctgctgcccg aggttgtgct gccggtgtgt gcgccggccc tgctgggcgg acgcacaaca
     4501 gtgactgcgg cagagctggc aaccttaccc ctgctgcagc aaagcacccg cccctatgcg
     4561 tggccgcagt ggctggaggc tgcgggggtt ggcggtgagg tcgatgacgc ggacagtgca
     4621 ccggccagcg tgctggccgg tccgcgctac gaacagtttt cgatggcggc cgcggtggca
     4681 gtgcacggtc ttggcgtggg gctgattccg cggctgctgg ttgaggctga gctggccagc
     4741 ggtgcgctgg tggtggccca tccgcagacc ctgcccggag agcgcttcta ttacctggtg
     4801 cagcccgagg gcacccaggt gcgccctgca ctgcagcgct tcattgactg gttacagcag
     4861 gccagcgccg gttaggcgct gcgccagaac atctcaaggc tggcggacga tgaccggctg
     4921 aatctggaac agctggtcac tgccagcatc cacggtcact ttcacccaat gcagactggg
     4981 gctgccaaag gtctgcaggc gggtcaggtt gggcagcagt ttggcggggc tgtacagcgg
     5041 cttgtccact ttgaaaaagt gggtgtcgcc atgcaccagc agcacctggc ccttgaaacc
     5101 ctcggtctgc ttgatcacct gtgccacgaa gttgcgataa ccattgaacc ggggctgctg
     5161 gctttcgtcc acctcctccg tctcgggcag atcaaagccg gggtctgcct ggatcaccag
     5221 caccacaccc tgggcaccgg tgtctttggc ttgctggaag gtttgctcca gccaaaccac
     5281 attggccgca tcccgctcca gatactctgc gttcgatgca tcgcactgcg ctggcgtgcg
     5341 cgcgctcttg ttggtgcact ctttggccga gagaaccacg ttgttgttgc tacccggcac
     5401 gttgagacct gcgaacacca cagggccatg gctgaagcgg gtgttttcca catatttttc
     5461 acccagcttg ccctggtgca gcaagggcat ggtggtctgg ccaaggctgt tggcagtggg
     5521 gaacatgatt ttgcgcaggt gcttgaggcg ctccagacca tcaaagccgc cgttgttggt
     5581 gcgatggcag tcggtccact cgttgtcacc gggtacgtag accacgggct tgaccagggt
     5641 gccaaacatt ttcacggcat cggtatagat atcgtcggtg catttcgaac tgccatcctt
     5701 gatatcaccg tcatagatgg agaaagcaat gttcgactgg ttgatgcttt gcagcactgc
     5761 aggcagtttg gactcgtcac ccgccttctt atagggcatg tcgccccaca caccgaaaga
     5821 ataggttccg tgttgtgcac cggggccggt ggcacaggcg gacagtccaa gggcaagcac
     5881 agcagcggtg agcagtcgga tcgacatggg aatttccagt gtggtcaaaa gtggcactgt
     5941 gcggcagccc ggtgacaggc gtgtgacgca tcgacaaccg tcctgccccc tgccgatgca
     6001 cggatagtgc aaacccctag atacaagatg gcacgtggat tgctagcatt cctttcagaa
     6061 gttgactaaa tggtcagatt tttgaacagg agttgtccgt gtccactacc ccccagcccg
     6121 atgtgcgccc aggccgcctc gccgccagcg aatacgcact gcgctttgcc gacgcctcgc
     6181 cccgcctgac ccattcgcag gcgctgcttg aggccgagcg ctgcctgtat tgctttgacg
     6241 ccccctgcgc cacggcctgc cccaccagca tcgatgtgcc gtcgttcatc aagcgcattg
     6301 ccgacgagaa cctgcgtggt gcagcccgca ccattctgga gagcaatccc ctgggcggca
     6361 tgtgcgcccg ggtgtgcccg accgagaacc tgtgcgaggc ggtctgtgtg cgcaataccc
     6421 aggagggcgt gcccgtggcc atcggccgcc tgcagcgcca cgcggtggac gcgctactgg
     6481 aaagcgccaa gccccagctc ttcacccgcg cagcccccac gggcaaaaag gtggcggtgg
     6541 tcggtgctgg ccccgccgga ctggcctgcg cccacacgct ggcgcgcatg ggccacgacg
     6601 tggtggtgtt tgatgcccgc cccaaagccg gcggcctgaa cgaatacggc ctggccagct
     6661 acaagacgcc ggacaacttt gc
//
LOCUS       DAOSJM010000092         6672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10416_length_6672_cov_4.297416, whole genome shotgun sequence.
ACCESSION   DAOSJM010000092 DAOSJM010000000
VERSION     DAOSJM010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6672
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10416_length_6672_cov_4.297416"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PKX82_04675"
     CDS             <1..456
                     /locus_tag="PKX82_04675"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOF51576.1"
                     /translation="SRPPTGDILAISRVKEIHARIPNTHLVMHGSSSVPQDLLAIINQ
                     YGGQMKQTYGVPVKEIQEAIKHGVRKINIDTDIRMAMTGAVRKFLAENPEKFDAREWL
                     KPAREAAKAVCKQRYLEFGCEGQGAKIKGDSLQVVAAKYARGELAQVVQ"
     gene            540..1448
                     /locus_tag="PKX82_04680"
     CDS             540..1448
                     /locus_tag="PKX82_04680"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOF51577.1"
                     /translation="MTNSLHTSTLGSLTLLARGKVRDNYAVGDDRILMVASDRLSAFD
                     VIMGEPIPGKGKLLTQMALFWFDKLGPKGMNICPIHLTGEAPESVVTAAEVPQVEGRS
                     MLVKRLKPLPVEAVVRGYLAGSGWKEYQENGAVCGVKLPAGLKNASKLPEPIYTPAAK
                     AAVGDHDENITFEKTVEMIGLDLATRIRDISIAIYKAAAQFALTKGIIIADTKFEFGL
                     DKDGTLTLMDEVLTPDSSRFWPLESYQEGTNPPSYDKQFVRDWLEQAQVDGKPWSKTP
                     PAPRVPNEVITRTAAKYQEALERLTT"
     gene            1519..2397
                     /locus_tag="PKX82_04685"
     CDS             1519..2397
                     /locus_tag="PKX82_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOF51578.1"
                     /translation="MTDPTDLPATPQAPIAYLTEPLKDLAAWVRYFSEAEIPVLADTA
                     IALEDLRAREDDVDPGMLAAVIEADPLLTIKFMAHVATKRRPGDVTETETVMSSLVMT
                     GVAPFFRNFGPQPTVEDRLHDQPEALEGLREILLRGSRAAHFAMAFAVHRGDTDVSVI
                     HQAAYLHDFAEMLMWCHAPTLALDIRAKQQANTTLRSANLQRFVYNIELDDLRQALMK
                     LWHLPALLVRISDGKHPDHPSVRNVVLAVRLARHTAHGWDNAAIPDDVDEIAQLLNAL
                     PRVALAFLRKVEPPPS"
     gene            complement(2413..2724)
                     /locus_tag="PKX82_04690"
     CDS             complement(2413..2724)
                     /locus_tag="PKX82_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HOF51579.1"
                     /translation="MTSTRLTRFFAALITGAALTAGAVAQTNTDLTPGEVRKVDTAAS
                     KLTIKHGDIKNLDMPAMTMVFQVRDPTLLAKVKAGDAVRFHAERGDGTYIVTSLEVVK
                     K"
     gene            complement(2737..3186)
                     /locus_tag="PKX82_04695"
     CDS             complement(2737..3186)
                     /locus_tag="PKX82_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HOF51580.1"
                     /translation="MKRRLLLGACMGALTGTSWAANTKPLVEIWKDPSCGCCEDWANH
                     MKQAGFPVQIHPEGNTAKRAAVGIPAQLGSCHTAMVAGYAIEGHVPAREVLRLLRQRP
                     QAIGLTVPGMPVGSPGMDGAIYQGRKDTYDVLLVARNGSTSVFQTYR"
     gene            complement(3183..3665)
                     /locus_tag="PKX82_04700"
     CDS             complement(3183..3665)
                     /locus_tag="PKX82_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HOF51581.1"
                     /translation="MKNTIKFIAVCALFSGASAAFGSGSHAGGHGHGAEAIGKPGVAT
                     QVTRTIAIEMSDAMRFTPDKITVRRGETIRFVVHNAGQLKHEFNLGTDADLKAHYALM
                     KKFPDMEHDDPNLVSLEPGKTGEVIWQFTKPGKVGFACLHVGHYEAGMKGTVTVGATK
                     "
     gene            complement(3686..5098)
                     /locus_tag="PKX82_04705"
     CDS             complement(3686..5098)
                     /locus_tag="PKX82_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HOF51582.1"
                     /translation="MHRRTFFNGAAATAMTAVAAASVSRVALAALPEPVLQGSPATQP
                     PLVPNTGRPYNPVVTLNGWTLPWRMNQGVKEFHLVAEPVVREMAPGFKAHMWGYNGQS
                     PGPTIEVVEGDRVRVLVTNKLPEHTSVHWHGQRLPNGMDGVSGLNQAPIPVGKTFVYE
                     FVARRPGTFMYHPHADEMTQMAMGMMGFWVTHPKVKNPLISEVDRDFCFLLNAYDIDP
                     GSMTPKTMTMLDFNLWSWNSRIFPGIDTLNVRHNDKVRIRVGNLTMTNHPIHLHGHEF
                     VVTGSDGGPWPLAARVPEITTDIAVGQMRQFEFLADEEGDWAFHCHKSHHTMNAMGHN
                     VPNLIGVDHRGLVGKLQKSMPDYMVMGERGMADMGEMEMPIPDNTAPMMTGRGPYGGV
                     EMGGMFSILKVRKDQKPGDYSNPGWFRQPAGTQAFEWTGPLPDPARFKAEGGSAMPRM
                     ETPATPTQVQIRKPSGHTGH"
     gene            complement(5133..6260)
                     /locus_tag="PKX82_04710"
     CDS             complement(5133..6260)
                     /locus_tag="PKX82_04710"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOF51583.1"
                     /translation="MALAIGASVAIAAPADGGVSDLQNSLAGKVLGVDAAPVRIARAG
                     VDGGPAVAALLEGPLGANAAVRIALLNNPELQVTLGSESTAISDITSREAPAKLRIRQ
                     TLTTLSATTLKAWIQAVTSAQSVDLLREAKATAHTRNELARRMVRVGNLSPLAQAQYQ
                     AALSDAALALARAEQEAFSAREKLIQLLGLWGEQTRFALPATLPALPEQVQDMPDVEA
                     RALAARADLAVARAQWDFKQRSSMPGTADDLWDAMADAARVRALAVKVRSQAREAYFN
                     YRSTFDMAQHLQTEVLPLRKYINDELLLRYNGMLTSVFDVLADSQQQTLSANAALLAQ
                     RDFWLAHTDLQAVLAGAPREALGTNAASEASNSAGPGPAGH"
     gene            complement(6290..>6672)
                     /locus_tag="PKX82_04715"
     CDS             complement(6290..>6672)
                     /locus_tag="PKX82_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51584.1"
                     /translation="HDHDLRVLDPGVDLDGGVADAREGDDRRARTLGSVLRERLEELA
                     LAERQLGDQLGGGHCALPAAPVPPVQYRSALDDLPKGVEQATTDWKAANATNGQFKRG
                     HADLLQWELDQARKRTSPAAGAQP"
BASE COUNT         1157 a         2113 c         2127 g         1275 t
ORIGIN      
        1 agccgcccac ccaccggcga catcctggcg atcagccgcg tgaaggaaat ccacgcccgc
       61 atccccaaca cccacctggt gatgcacggc tccagcagcg tgccacagga cctgctggcc
      121 atcatcaacc agtacggcgg ccagatgaag cagacctacg gcgtgccggt caaagagatc
      181 caggaagcca tcaagcacgg tgtgcgcaag atcaacatcg acaccgacat ccgcatggcg
      241 atgaccggcg cggtgcgcaa gttcctggcc gagaaccccg agaagttcga cgcccgcgag
      301 tggctcaagc ccgcccgcga agccgccaag gccgtgtgca agcagcgtta cctggagttc
      361 ggttgcgaag gccagggcgc caagatcaag ggcgacagcc tgcaggtcgt tgctgccaaa
      421 tacgcccgcg gcgaactggc gcaagtggtg cagtaaagac cagcccctcc ctgcgataat
      481 tgcaggcccg tcgacaagca caagcgcttg cggacgggcc ttttctaatt ctggatgcca
      541 tgaccaattc cctgcacacc tccaccctgg gctccctgac attgcttgcg cgcggcaaag
      601 tgcgtgacaa ctatgccgtg ggcgatgacc gtatcctgat ggtggccagt gaccggctca
      661 gtgcttttga tgtcatcatg ggcgaaccca ttcccggcaa gggcaaactg ctcacgcaga
      721 tggcactatt ctggtttgac aagctgggcc caaagggcat gaatatctgc cccatccacc
      781 tgactggcga ggctccggag agcgtagtga cggcagcgga agtgccccag gtggaaggcc
      841 gctccatgct ggtcaagcgc ctcaagcccc tgcccgtgga ggccgtggtg cgcggttacc
      901 tggccggcag cggctggaag gaataccagg aaaacggcgc cgtctgcggt gtcaagcttc
      961 ctgctggctt gaagaacgcc agcaagctgc cggagcctat ctacaccccc gccgccaaag
     1021 ccgccgtggg cgaccacgac gagaacatca cgttcgagaa aaccgtcgag atgattggcc
     1081 tggacctggc cacccgcatc cgcgacatca gcatcgccat ctacaaggcg gcggcccagt
     1141 ttgcgctgac caagggcatc atcattgccg acaccaagtt cgagtttggc ctggacaagg
     1201 acggcacgct gacgctgatg gatgaggtgc tgacgcccga ctcctcccgc ttctggccac
     1261 tggaaagtta ccaggaaggt accaacccgc ccagctacga caagcagttc gtgcgggact
     1321 ggctggagca ggcgcaggtg gacggcaagc cctggagcaa aaccccaccg gccccgcgcg
     1381 tgcccaacga ggtgatcacc cgaactgcag ccaaatacca ggaagcgctg gaacggctga
     1441 ccacctgaga gatgcacgca gggcttgacc tgtcgcgggg gcgcgcagat actgcgccca
     1501 aacgactacc ggcatgtttt gaccgacccc actgacctgc ctgccacccc gcaggcgccc
     1561 attgcctacc tgaccgagcc gctcaaggac ttggcggctt gggtgcggta cttcagcgaa
     1621 gccgaaatcc ctgttcttgc cgataccgcc atcgccctcg aagatctgcg tgccagagaa
     1681 gacgatgtgg acccgggcat gcttgcagcc gtgatcgagg ccgatcccct tctgaccatc
     1741 aagttcatgg cccatgtggc caccaagcgg cgaccaggcg atgtgaccga gacggaaacc
     1801 gtcatgagtt cactggtcat gaccggggtt gcgccttttt tccggaattt tggtccccag
     1861 cccacggtgg aggaccgctt gcacgaccag cccgaagccc tggaaggcct gcgtgaaatc
     1921 ttgctgcgcg gcagcagagc ggcccatttc gccatggcgt ttgcggtgca ccgtggcgac
     1981 accgacgtgt cggtcatcca ccaggccgcc tacctgcatg attttgcgga aatgctgatg
     2041 tggtgccatg cgcccaccct ggcactcgat atccgcgcca agcagcaggc caacaccact
     2101 ctgcgttcag ccaacctgca aagatttgtc tacaacatcg aactcgacga cctgcgccag
     2161 gccctcatga agctgtggca cctgccggcc ctgctggtgc gcatctcgga cggcaagcac
     2221 cccgaccacc ccagtgtgcg caacgtggtg ctggcggtgc gcctggcacg ccacacagcc
     2281 cacggctggg acaacgccgc cattcccgac gatgtcgatg agattgcgca attgctcaat
     2341 gcgctgccgc gcgtggcgct ggcattcctg cgcaaggtgg agccgccgcc gtcctgaccc
     2401 gctgcggacc gcttactttt tgaccacttc caggctggtc acgatgtagg ttccgtcacc
     2461 gcgttcggcg tggaagcgca cagcgtcacc ggcttttact ttggccagca atgtggggtc
     2521 gcgcacctga aacaccatgg tcatggcggg catgtccagg ttcttgatgt cgccgtgctt
     2581 gatggtgagc ttgctggccg ctgtatccac cttgcggact tcgcccgggg tcaggtctgt
     2641 gttggtctgg gctaccgcac cggcggtcag ggcggcgccg gtgatcaatg cagcaaagaa
     2701 acgtgtcaat cgggtgcttg tcatgggaga actcctctag cggtaggttt gaaaaacaga
     2761 agtgctgcca tttctggcaa ccagcagcac gtcgtacgtg tccttgcggc cctggtagat
     2821 cgcgccatcc ataccgggtg agcctacggg catgcccggt acggtcaggc caatggcctg
     2881 ggggcgctgg cgcaacagcc gcaggacctc cctggctggc acatggccct caatcgcgta
     2941 tcccgccacc atggcggtgt ggcaggaccc cagttgcgcc ggtatgccca ctgcggcgcg
     3001 cttggccgta ttgccttccg ggtggatttg caccggaaag ccagcctgct tcatgtggtt
     3061 ggcccagtct tcgcaacagc cgcagctggg gtctttccag atttccacca gaggcttggt
     3121 gtttgcagcc caggacgtgc ccgtgagtgc gcccatgcag gcgccaagga gtaatcggcg
     3181 tttcattttg tggctcctac ggtgacggtg cccttcatgc cagcctcgta gtggcccaca
     3241 tgcaggcagg caaaccccac tttgccgggt ttggtgaatt gccagatgac ctccccggtc
     3301 ttgccgggct ccaggctgac cagattgggg tcatcgtgtt ccatatccgg aaacttcttc
     3361 atcagcgcat agtgggcctt gaggtcagca tcggtgccca ggttgaactc atgcttgagc
     3421 tggccggcgt tgtgcacgac gaaacggatg gtctccccgc gccggaccgt gatcttgtcc
     3481 ggagtgaagc gcatggcgtc ggacatctcg atggcgatgg tgcgcgtgac ctgggttgcc
     3541 acacccggtt tgccgatggc ctcggctcca tggccatggc ctccggcgtg gcttcctgag
     3601 ccaaaagcgg cgctagcccc cgaaaatagt gcgcacacag ctatgaattt gatagtattt
     3661 ttcatggtat ttttgaaatt gaaaatcagt gaccagtgtg tccactgggc ttgcgtatct
     3721 ggacctgggt gggcgtggcg ggggtttcca tgcgtggcat ggcgctgccc ccctccgcct
     3781 tgaagcgggc cgggtccggc aaaggaccgg tccattcaaa ggcctgtgtg ccggcaggtt
     3841 gcctgaacca gccggggttg ctgtagtcac cgggcttctg gtccttgcgc actttgagga
     3901 tgctgaacat gccacccatc tccacgccgc cataggggcc gcggcccgtc atcatgggcg
     3961 cagtgttgtc gggaatgggc atttccattt cgcccatgtc ggccatgccg cgctcaccca
     4021 tcaccatgta gtccggcatg gatttttgca gcttgccgac caggccgcgg tggtctacgc
     4081 caatcaggtt gggcacgttg tggcccatgg cgttcatggt gtggtggctc ttgtggcagt
     4141 ggaaggccca gtcgccttcc tcgtcggcca gaaactcgaa ctgccgcatc tgccccaccg
     4201 caatgtcggt ggtgatttcg ggcacgcgcg ccgccagtgg ccaggggccg ccgtcgctgc
     4261 cggtcaccac aaactcgtgg ccatgcaggt ggatggggtg gttggtcatg gtcaggttgc
     4321 ccacgcggat gcgcaccttg tcgttgtgcc gcacgttcag cgtgtcgatg ccggggaata
     4381 tccggctgtt ccagctccag aggttgaagt ccagcatggt catggtcttg ggcgtcatgc
     4441 tgccggggtc gatgtcgtag gcgttgagca ggaagcagaa atcgcggtcc acctcgctga
     4501 tgagcggatt tttgactttg gggtgcgtca cccagaagcc catcatgccc atggccatct
     4561 gcgtcatttc atccgcatgc gggtggtaca tgaaggtgcc ggggcggcgc gccacaaatt
     4621 cgtagacaaa ggtcttgccc acaggaatgg gtgcctggtt cagacccgac acgccgtcca
     4681 tgccattggg caggcgctgg ccgtgccagt gcacgctggt gtgctctggc agcttgttgg
     4741 tgacaagcac acggacccgg tcgccctcga ccacctcgat ggtggggccg gggctctggc
     4801 cgttgtagcc ccacatgtgg gccttgaagc cgggcgccat ttcgcgcacc accggttccg
     4861 cgaccaggtg aaattccttc acgccctggt tcatgcgcca gggcagggtc cagccgttga
     4921 gcgtgaccac cgggttgtag ggccggccgg tgttggggac cagcggcggc tgggtggcgg
     4981 gcgagccttg cagcaccggt tcgggcaagg cggccagagc cacccggctg actgaagctg
     5041 cggctacggc cgtcatggca gtcgctgcgg ccccgttgaa aaatgtacgt cgatgcatgg
     5101 atgatttttg tgtcatggtg tgggcactcc gttcagtggc cggcagggcc tgggcccgcg
     5161 gaattggatg cttcggaggc tgcattcgtg cccagcgctt cgcggggtgc gcccgccagc
     5221 acggcctgca ggtcggtatg ggccagccag aagtcacgtt gcgccagcag tgcggcgttg
     5281 gcgctcaggg tttgctgctg gctgtcagcc agcacgtcaa acacactggt caacatgccg
     5341 ttgtagcgca gcaggagttc gtcgttgatg tacttgcgca atggcagcac ttcggtttgc
     5401 aggtgctgcg ccatgtcgaa ggtgctgcgg tagttgaaat acgcctcccg ggcctggctg
     5461 cgcaccttta ccgccagtgc acgtacccgt gcagcatcgg ccatggcatc ccacagatca
     5521 tccgcagtgc cgggcatgga cgacctttgc ttgaagtccc actgcgcgcg cgccacggcc
     5581 agatcggcac gtgcggccag ggcccgtgcc tccacatcgg gcatgtcctg cacctgttcc
     5641 ggcagtgcgg gcagtgtggc gggcaacgca aagcgggtct gttcccccca caagcccagc
     5701 aactggatca gcttttcgcg ggcgctgaaa gcctcctgtt ctgcgcgcgc cagcgccagc
     5761 gctgcatcgg acagggcggc ctggtattgg gcctgcgcca gcggactcag gttgcccacc
     5821 ctgaccatgc gccgggccag ttcattgcgg gtgtgtgccg tggccttggc ctcccgcagc
     5881 aagtccacac tctgcgcgct ggtcacggcc tgaatccagg ctttcagcgt agttgccgac
     5941 agggtcgtca gcgtttgtcg gatacgcagc ttggccggcg cctcgcgact ggtgatgtcg
     6001 gagatggctg tggattcact gcccagggtg acttgcagct ccgggttgtt cagcaaggcg
     6061 atgcgcaccg ccgcgttggc gcccagtggc ccttccagaa gcgccgcaac ggcgggaccg
     6121 ccatcgacac cggcgcgggc gatgcgcacc ggtgcagcat ccacaccgag cactttgcca
     6181 gccagactgt tctgcaggtc ggagacgccg ccatcggcag gtgcggctat ggcaacactg
     6241 gcgcctattg ccagcgccat ggccgcccaa aggtgttgca gccgggccct catggttggg
     6301 ctcccgcggc tggcgatgtg cgtttgcgcg cctggtccag ttcccactgc agcaggtccg
     6361 cgtggccgcg cttgaattgg ccgttggtgg cattggcggc tttccagtcg gtggtggcct
     6421 gttccacccc ttttggaagg tcgtccagtg ccgagcggta ttgcaccggt ggcacgggcg
     6481 cggcgggcag ggcgcagtga ccgccgccaa gctggtcgcc gagctggcgc tcggccaggg
     6541 caagttcttc caggcgttcc cggagtacgg acccgagcgt tcgggcgcgc cgatcgtcgc
     6601 cttcacgcgc gtcagcgacg cccccatcca ggtctactcc ggggtcgagc acccgcagat
     6661 cgtggtcgtg ct
//
LOCUS       DAOSJM010000093         6662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10437_length_6662_cov_5.273044, whole genome shotgun sequence.
ACCESSION   DAOSJM010000093 DAOSJM010000000
VERSION     DAOSJM010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10437_length_6662_cov_5.273044"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PKX82_04720"
     CDS             complement(<1..248)
                     /locus_tag="PKX82_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOF51585.1"
                     /translation="MTTSLPTVGHLIGGKLVQGANPNPRSQDVFNPATGKAEKRVLLA
                     DKTTVELAIANAQAAFPAWRNTPPLKRARVMGNLKVLL"
     gene            complement(337..1713)
                     /locus_tag="PKX82_04725"
     CDS             complement(337..1713)
                     /locus_tag="PKX82_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOF51586.1"
                     /translation="MKLTDNASQPVTSTTPYPQDRAFLEAHWMPYSANRNFKATGAQS
                     GRMMVSAQGCYYTDSNGKKIFDGLSGLWTCGLGHGRTEIAEAIGRQAATLDYSPAFQY
                     GHPLSFELANRIRELTPDGLDYVFFTGSGSESADTSLKMARAYWRAKGMGTKTRLIGR
                     EKGYHGVNYGGISVGGIGGNRKIFGQGIEADHLPHTQPAAGSFYKGMPPTGMELADEL
                     TRLIALHDASNIAAVIVEPMSGSAGVVIPPVGYLQRLREICTANNILLIFDEVITGFG
                     RMGAYTGASAFGVTPDIANFAKQITNGAQPLGCVVAKKDIYDTFMAQGGPDYMLEFPH
                     GYTYSAHPVACAAGIAALDILVKENMVERAAALAPHFEKAVHSLKGAKHVLDIRNYGL
                     AAGFTIASAPGEPAKRPYEIAMQCLEKGFYVRYGGDTIQLAPPFIATPAEIDSLVNAL
                     GESLNATA"
     gene            1817..2797
                     /locus_tag="PKX82_04730"
     CDS             1817..2797
                     /locus_tag="PKX82_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012217032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF51587.1"
                     /translation="MQVNTLPEKASATQPKARAMLGQLSDMDLRLLRVFRTVVDCGGM
                     AAAELELNIGTSTVSRHVKDLETRLGLTLCRRGRAGFALTPEGEKIYAQTTHLLAAAE
                     AFRTGVDEIHQRMGGQLHIAVFDKTASNPNAHIAQAIALFTRQAPDVALNLHVGTLTA
                     IERGVLDGTFQIGIIPGHRSSDALEYTPLFDEAMSLYCGRAHALFAQTGVTLGWDRLR
                     EQQFAGLGYHSPNMDLTLRQRLPRSATGFDQESIATLILSGQFLGFLPDHYAQSFVRD
                     GQMRAIEPEVLRYDCQFFAITRRSPQPARATRAFQACLREAHDADRSSMP"
     gene            complement(2746..4086)
                     /locus_tag="PKX82_04735"
     CDS             complement(2746..4086)
                     /locus_tag="PKX82_04735"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019478 - D-amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HOF51588.1"
                     /translation="MKITILGAGIIGISTAWHLAERGHEVTVVDRQPDAALETSFANA
                     AQISVSYCEPWANREAPLKALKWMFSKEAPLLFRPQLPIGEGFHQYRWGLEFLANCND
                     TAFERNVQQLVALGSYSHAALKDVVAATGIVYNRLEKGIAHFYLDQKSFDDAGRAAAL
                     MAKYGVNRRVVSKEELLKIEPAFQLFANQIVGGTYTASDESGDARVFTQELAQRCAAR
                     GVQFLWNHDVVSLNAADDGIDTVAVRNRTTGRNEALSADHFVVAMGSYTAPLLRTVGV
                     NLPIYPGKGYSATFQILKPELAPQVSFIDDSVKCAVSRLGDQLRIAGTIEVGGYSLDL
                     DGSLARARCAMLARRVETLFPGVCDTRSVEEGGNPNYWTGLRPATPTNIPYIGKTKVG
                     KLWVNAGHGTLGWTHGAGSGKAMAELISGRQPAMKFGFYGMDERSASWASLKHA"
     gene            4174..5109
                     /locus_tag="PKX82_04740"
     CDS             4174..5109
                     /locus_tag="PKX82_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HOF51589.1"
                     /translation="MRSFDTDALECLAAIVEEGGFERAAVRLSITQSAVSQRLRALEA
                     QVGTVLLVRSRPLKPTAAGRLLIKHAMQMRLLRADLETDLHELTPDSGNSREEERISI
                     AINADSISTWVLPALGPLVSEGLPLEIITDDQDFTHEWLREGQVLGCVTTLKKALRGC
                     KVLPLGAMQYVAVASRDYARTHCPQGLGAHNFRQIPFIAFNRKDDLQVEFVGRALGLR
                     RVALSQRFVPSSEGQVRAALAGWGATVVPELQVRDLLAQGELVNLAPQASLPVSLYWH
                     CWNLHSVVIDRLTAALAGAAAKALTGAPQNHIVAK"
     gene            5152..5769
                     /locus_tag="PKX82_04745"
     CDS             5152..5769
                     /locus_tag="PKX82_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOF51590.1"
                     /translation="MKSTPVVKVSFKQQMLQAREDAIIRAVNLLLAEKGFDAMTVDEV
                     AAAVGIAKASLYKHFPSKEDLACAAMVRVMQRAQDYIGTLPQDDTPLNKLRAVVRWTM
                     ELKLAGEMPSLPSQNSSLRATLVASRDYMDGLMEVSDELGAWIEAAQAQGAINSAIPA
                     IAVLYTLYARACDPVLEFLKMGGQYDDARIVELVLSTCFDGLNAR"
     gene            complement(5766..6188)
                     /locus_tag="PKX82_04750"
     CDS             complement(5766..6188)
                     /locus_tag="PKX82_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF51591.1"
                     /translation="MKILLAVDGSTFSKKMLAYLATHDTLFTKDHDYTVFTAQPALPP
                     RARAAVGKDVVESYYAEESEKVLGPVSKFLVRHGIDAKSTWKVGHAGEAISKLAEQGK
                     FDLVVMGSHGHGSLVKLVMGSVATEVLAHCKVPVLLVR"
     gene            6298..>6662
                     /locus_tag="PKX82_04755"
     CDS             6298..>6662
                     /locus_tag="PKX82_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HOF51592.1"
                     /translation="MRLRASPENHVSSGPAVNRRTLCLSLGGLVAVLVLPRPVAAATT
                     APPLMLATDFRKDLPLADYWVSEKYDGVRGYWDGQRLVTRAGNPIAAPAWFTAGWPAT
                     PMDGELWAGRGQFSQAVSVV"
BASE COUNT         1184 a         1981 c         2206 g         1291 t
ORIGIN      
        1 tccagcagca ccttgagatt gcccatgacg cgggcgcgct tgagcggcgg cgtgttgcgc
       61 caggcgggga aggcggcctg tgcgttggca atggccagct ccaccgtggt tttgtcggcc
      121 agcagcacgc gcttttcggc cttgccggtg gcggggttga agacgtcctg tgagcgcgga
      181 ttcgggtttg cgccctggac taacttgcca ccaatcaggt ggccaacggt aggaagagaa
      241 gtggtcatgt caatggtgca atcaagagta aaaatggcca gtagcccccg tacactgtgc
      301 gcaggcagct actaaaataa tagcaattat cggtggtcag gctgttgcgt tgagcgactc
      361 gcccaatgcg ttgaccaggc tgtcaatctc tgcaggggtg gcaataaacg gcggcgccag
      421 ctggatggta tcgccgccgt agcgtacata gaaacctttt tccagacact gcatggcaat
      481 ttcgtacgga cgtttggcgg gctcaccagg agcggatgcg atggtgaagc cggccgccag
      541 tccgtagttg cggatgtcca gcacatgctt ggcgcctttg aggctgtgca cggctttctc
      601 gaaatgcggc gccagggcag cggcgcgctc caccatgttt tccttgacca gaatgtccag
      661 tgcggcgatg ccggccgcgc aggccacggg gtgggcagag taggtgtagc cgtgcgggaa
      721 ttcgagcatg tagtccgggc cgccctgcgc catgaaggtg tcgtagatgt cctttttggc
      781 gaccacgcag cccaggggtt gcgcgccatt ggtgatctgt ttggcaaagt tggcaatgtc
      841 aggggtcacg ccaaatgccg aggcaccggt gtaggcgccc atgcggccaa agccagtgat
      901 cacttcgtca aaaatcagca ggatgttgtt ggcggtgcag atctcgcgca ggcgctgcag
      961 gtagcccacg ggcggaatca ctacgccagc ggagccagac atgggctcca caatcacggc
     1021 cgcaatgttg gacgcatcat gcagcgcaat caggcgggtc agttcatcgg ccagctccat
     1081 accggtgggt ggcatgccct tgtaaaagga gcctgcggcc ggctgggtgt ggggcaggtg
     1141 atcggcctca ataccctggc cgaaaatctt gcggttgcca ccgatgccac cgacggagat
     1201 gccgccgtag ttcacaccgt ggtagccctt ttcgcggcca atcaggcggg tcttggtgcc
     1261 cattcccttg gcgcgccagt aggcgcgggc catcttcagc gaggtgtcgg ccgactcgga
     1321 gccggagccg gtgaagaaca catagtccag cccgtcgggc gtgagttcgc ggattcggtt
     1381 ggccagctcg aacgacagcg ggtggccgta ctggaacgcg ggggagtagt ccagcgtggc
     1441 ggcctggcgg ccaatggcct ccgcaatctc ggtgcggcca tggcccaggc cgcaggtcca
     1501 caggcctgag aggccgtcaa aaatcttctt gccattgctg tcggtgtagt agcagccctg
     1561 ggcgctgacc atcatgcgcc cggactgggc gccagtggcc ttgaagttgc ggttggcgct
     1621 gtagggcatc cagtgtgcct ccaggaaagc gcggtcctgg gggtagggcg tggtgctggt
     1681 cacgggctgg ctggcgttgt cggtcaattt catgggatct cctggggcgg tgtggtgtgc
     1741 ccaaaatggt ttgtggattg tgggtggctc tgttactctt ataaagtcac aaatatcact
     1801 acttggttgc agatatatgc aagtaaatac tctccccgaa aaggcttctg ctactcaacc
     1861 caaagcacgc gccatgctgg gccagctcag cgacatggac ctgcgcctgc tgcgggtgtt
     1921 ccgcacggtg gtggattgcg ggggcatggc ggcggccgag ctggagctca atatcggcac
     1981 ctccaccgtc agccgccatg tcaaggacct ggaaacccgg ctgggcctga ccctgtgccg
     2041 gcgcggtcgg gccggttttg cgctcacgcc cgagggcgaa aagatttacg cgcagacgac
     2101 ccacctgctg gccgccgcgg aagcgttccg caccggggtg gacgaaatcc accagcgcat
     2161 gggcggccaa ctgcacattg cggtgtttga caagacggcc agcaacccga acgcccacat
     2221 cgcgcaggcc atcgcgctgt tcacccggca ggcgccggac gtggcactga acctgcacgt
     2281 gggtacgctc accgcgatcg agcgcggcgt gctggacggc acctttcaga tcggcatcat
     2341 cccagggcac cgcagttcgg acgcactgga gtacacgccc ctgtttgacg aagccatgtc
     2401 gctctactgc ggccgtgcgc atgcactgtt tgcccagact ggggtcacgc tgggctggga
     2461 ccggttgcgg gagcagcagt ttgccggact gggctaccac tcgccgaaca tggatctcac
     2521 gctgcgccag cggctgccgc gcagtgcgac cgggtttgac caggagtcga ttgccacgct
     2581 gatcctgtcc ggccagtttc tgggctttct gcccgaccac tatgcgcaaa gctttgtgcg
     2641 cgacgggcag atgcgggcca tcgagcccga ggtgctgcgc tacgactgcc agttcttcgc
     2701 catcacccgg cgctcaccgc aacccgcgcg ggcgacgcgg gcgtttcagg cgtgtttgag
     2761 ggaggcccac gatgcggacc gctcatccat gccgtagaag ccaaacttca tggcaggctg
     2821 gcggccgctg atcagctcgg ccatggcttt gcccgatccg gcgccatggg tccagcccag
     2881 ggtgccgtgg ccggcgttga cccagagctt gcccactttg gtcttgccga tgtaggggat
     2941 gttggtcggt gtggcggggc gcaggccggt ccagtagttc gggttgccgc cttcttcgac
     3001 actgcgggtg tcgcagacgc cggggaacag ggtttccacc cgtcgcgcca gcatggcgca
     3061 gcgggcgcgg gccagggagc catcaaggtc gaggctgtag ccacccactt ctatcgtgcc
     3121 ggcaatgcgc aactggtcgc ccaggcggct cacggcgcat ttaaccgaat cgtcaatgaa
     3181 gctcacctgc ggggccagtt cgggcttgag gatctggaag gttgcgctgt agcccttgcc
     3241 ggggtaaatg ggcaggttga cgcccacagt gcgcaacagc ggcgcggtgt aggaacccat
     3301 ggccaccaca aagtggtcgg ccgacagcgc ttcattgcgg ccggtggtac ggttgcgcac
     3361 ggccacggtg tcgatgccat cgtccgcagc gttgagcgac accacgtcgt ggttccacag
     3421 gaactgcact ccgcgtgcgg cgcagcgttg ggccagctcc tgggtgaaga cgcgcgcatc
     3481 gccactctcg tcgctggcgg tgtaggtgcc gcccacgatc tggttggcaa acagctggaa
     3541 tgcgggctcg atcttcaata gctcttcctt gctgaccacg cggcggttga cgccgtactt
     3601 ggccatcagc gccgcggcgc ggcctgcgtc gtcaaacgac ttctggtcca ggtagaagtg
     3661 ggcaatgcct ttttccagcc ggttgtagac gatgccggtg gccgcgacca cgtccttgag
     3721 ggcggcgtgg ctgtaggagc ccagcgccac gagctgctgt acgttgcgct cgaatgccgt
     3781 gtcgttgcag ttggccagaa actccaggcc ccagcggtac tggtggaagc cttcgccaat
     3841 cggcagttgc ggacggaaca gcagaggggc ttctttgctg aacatccatt tcagggcctt
     3901 gagtggggct tcgcggttgg cccagggctc gcagtagctg accgaaatct gtgccgcatt
     3961 ggcaaagctg gtctccagcg ccgcatcggg ctgccggtcc acgacagtga cttcgtggcc
     4021 gcgctctgcc agatgccatg ctgtactgat gccaatgatg cctgcgccaa ggatggtgat
     4081 tttcatggcg ccagtgtcgt ggaaagtgct ttggagtaaa agtaaaatta cccaaattac
     4141 aatttcatta gtctgactaa tactggatac gccatgcgct ccttcgatac cgatgccctg
     4201 gaatgcctcg ccgccattgt ggaggagggc ggatttgagc gcgcggccgt gcggctgtcg
     4261 atcacccagt cggcagtgtc gcagcgactg cgtgcgctgg aagcccaggt cggcaccgtg
     4321 ctgctggtgc gcagccggcc cctcaaaccg accgctgcgg gtcgcctgtt gatcaagcac
     4381 gcgatgcaga tgcggctgct gcgggccgac ctggagaccg acctgcatga gctgacgcct
     4441 gattcgggaa actcgcgtga ggaagagcgc atctccatcg ccatcaacgc cgacagcatt
     4501 tccacctggg tgctgcccgc actggggccg ctggtcagtg agggcttgcc cctggaaatc
     4561 atcacggacg accaggactt tacccatgag tggctgcgcg aggggcaggt gctgggctgc
     4621 gtcactaccc tcaagaaggc gttgcgcggc tgcaaggtgt tgcccctggg tgccatgcag
     4681 tatgtcgccg tggcgagccg cgactatgcg cggacgcatt gcccacaggg cttgggcgcc
     4741 cacaattttc gccagattcc gttcattgcg ttcaaccgca aggacgacct gcaagtggag
     4801 tttgtggggc gtgccctggg tttgcgccgc gtggccctga gccagcggtt tgtgcccagc
     4861 tccgagggcc aggtgcgtgc cgcactggcc ggctggggtg cgaccgtggt acccgagttg
     4921 caggtgcgcg acttgctggc gcagggggag ctggtgaatc tggcgcccca ggcgagcttg
     4981 ccggtcagtc tgtactggca ctgctggaac ctgcattcgg tcgtgatcga ccggctgacg
     5041 gcggcactgg ccggtgccgc tgccaaggcg ttgacgggcg ccccccaaaa ccacatagtc
     5101 gcaaaatagc cttcaactac acaaaaagac gggaacggct aaacttcaga catgaaatcc
     5161 acgcccgtag tcaaagtttc cttcaagcag cagatgctgc aggcacgcga ggatgcgatc
     5221 atccgcgctg tcaacctctt gctggccgag aaaggctttg acgccatgac ggtggacgaa
     5281 gtggcggctg ccgtgggcat cgccaaggcc agcctgtaca agcattttcc cagcaaggaa
     5341 gacctggcct gcgcggccat ggtgcgggtc atgcagcgcg cccaggacta cattggcacc
     5401 ctgccacagg acgacacacc attgaacaag ttgcgcgccg tcgtgcgctg gaccatggag
     5461 ctcaaactgg cgggcgaaat gccttcactg cctagccaga attccagcct gcgtgccaca
     5521 ctggttgcga gccgggacta catggatggg ctgatggaag tcagcgatga gcttggcgcc
     5581 tggatcgagg cggcccaggc acagggcgcg atcaattccg ccatccctgc catcgcagtg
     5641 ctctacacgc tgtatgccag agcctgcgac ccggtgctgg agttcctgaa gatgggtggc
     5701 cagtatgacg atgcgcgcat cgtggaactg gtcctgtcca cctgctttga cggactgaac
     5761 gcccgttagc gcaccagcag cacggggact ttgcagtggg ccagcacttc ggtggcgact
     5821 gagcccatca ccagcttcac cagggagccg tggccgtgtg aacccatgac taccaggtcg
     5881 aatttgccct gctcggccag tttggaaatc gcctcacccg cgtggcccac cttccaggtg
     5941 cttttggcgt cgatgccatg gcgcaccaga aacttggaca cgggccccag caccttttcg
     6001 ctttcctcgg cgtagtacga ctccaccacg tccttgccca ctgcagcgcg tgcgcgcggg
     6061 gggagcgcgg gctgggcagt gaatacggtg tagtcgtggt ctttggtgaa cagggtgtcg
     6121 tgtgtggcca ggtaggccag cattttcttg ctgaacgtac tgccgtctac ggcgagaaga
     6181 atcttcatgt aaatctcctg aatgttggtt ccagtgtaac gaaggacgtt gccatatcga
     6241 agccctttgg tatcctgtgg ggataaggat ttcaagaaga ccaaggggga ggacaccatg
     6301 cgtctgcgtg catcgccaga gaaccacgtt tcatcgggcc cggctgtaaa ccggcgcacc
     6361 ttgtgcctgt cgctgggcgg gctggtcgcc gtgctggtac tcccacggcc cgtcgctgcg
     6421 gctaccactg cgccgccctt gatgttggca actgactttc gcaaggacct gccgctggca
     6481 gactactggg tgagcgaaaa atacgatgga gtgcgcgggt actgggatgg tcagcggctg
     6541 gtgacgcgcg ctggcaaccc gattgctgcg ccggcctggt ttaccgcagg ctggcccgcc
     6601 actccgatgg atggcgaact gtgggcgggg cgcggccagt tcagccaggc ggtctcggtg
     6661 gt
//
LOCUS       DAOSJM010000094         6659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10443_length_6659_cov_4.453210, whole genome shotgun sequence.
ACCESSION   DAOSJM010000094 DAOSJM010000000
VERSION     DAOSJM010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10443_length_6659_cov_4.453210"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="PKX82_04760"
     CDS             <1..289
                     /locus_tag="PKX82_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOF51593.1"
                     /translation="IASDPIVWRKDLPEATKTKLKNFFLNYAKTNPQEREVMAKMNWS
                     MFKESSNAQLVPIRQLDFFSKRNKIESDTTLSADDRKLRLAEIDKKLAELK"
     gene            310..1116
                     /gene="phnE"
                     /locus_tag="PKX82_04765"
     CDS             310..1116
                     /gene="phnE"
                     /locus_tag="PKX82_04765"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOF51594.1"
                     /translation="MQDAASLSTQTTALAAVPKRSLGWHLSWAVLIGLLLASWKGADM
                     RPLDLWRDSGNMATYVADFFPPNFSQWRMYVEEMVVTLQIALWGTALAVITAIPMALM
                     ASANLVPWWVYQPTRRLMDATRAINEMVFAMLFVVAVGLGPFAGVLALWIHTTGVLAK
                     LFSEAVEAIDPQPVEGIRSTGASALHEIIYGVIPQVMPLWISFTLYRFESNVRSASVV
                     GMVGAGGIGVVLWEIIRGFQYAETCAVMIIIVLTVSMIDVVSARIRKVLV"
     gene            1129..1680
                     /locus_tag="PKX82_04770"
     CDS             1129..1680
                     /locus_tag="PKX82_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOF51595.1"
                     /translation="MALSIDDILQLFQRRARKQYGREAVSQMEHALQCAQLAEEAGET
                     SETIVASLLHDVGHLIAAENADRVDDTQEHDDLHEYIALPFLRSVLPDAVLEPIRLHV
                     EAKRYLCIADPGYWDTLSPASKHSLEQQGGPYSQDAAAAFLLQPFAQEAIRLRRYDDF
                     AKVPQKATPDLAYFATRLRAVAL"
     gene            complement(1682..2521)
                     /gene="kynA"
                     /locus_tag="PKX82_04775"
     CDS             complement(1682..2521)
                     /gene="kynA"
                     /locus_tag="PKX82_04775"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HOF51596.1"
                     /translation="MTSTEKIVADEKALLDFSRSMSYGDYLQIDTILSAQKPLSPAHD
                     EMLFIVQHQTSELWMKLMLHELGAAIQCIARDELGTAFKMLARVSKIMEQLVHAWDVL
                     ATMTPPEYSAIRPYLGQSSGFQSFQYRCIEFSLGNKNAAMLKPHAHAPERLALVQAAY
                     AAPSLYDEALRLLARRGVAVPESHTQRDWTQPYEASDAVEQAWLTVYREPQKYWDLYQ
                     LGEELTDLEDAFRLWRFRHVTTVERVIGFKRGTGGTGGVSYLRKMLDVVLFPEIWTLR
                     TGL"
     gene            complement(2639..3928)
                     /gene="kynU"
                     /locus_tag="PKX82_04780"
     CDS             complement(2639..3928)
                     /gene="kynU"
                     /locus_tag="PKX82_04780"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HOF51597.1"
                     /translation="MTTLHDCQQRDAADPLRHLRNQFTIPDGVIYLDGNSLGVMPKKA
                     AARAAEVVNQEWGQGLIRSWNTAGWFSLPQRLGNRISPLIGAGQDEVVVTDSTSINLF
                     KVLSAALSITAQDHPEKRVIVSERSNFPTDLYIAEGLCKERGFTLQLVEPEEIAVALT
                     RDVAVLMLTHVNYRTGAMHDMAAVTAAAHAAGALTIWDLAHSAGAVPVDLRGAGADFS
                     IGCGYKYLNGGPGAPAFVWVNPRHADRFWQPLSGWWGHASPFAFTADYQPAHGIARYL
                     CGTQPIVSLSLLECGLEGFEAAAPLGGMAALRTKSLALTDLFIQLVEERCQGYGLGLA
                     TPRDHAQRGSQVCLTREHNAEGSGAFAIVQALIARGVIGDFRAGDGGIHKDILRFGFT
                     PLYIGYEDVWNAVEHLRQVLETAEWKKPEFNQKHAVT"
     gene            complement(4023..4223)
                     /locus_tag="PKX82_04785"
     CDS             complement(4023..4223)
                     /locus_tag="PKX82_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51598.1"
                     /translation="MSQNDNDEPPLWLTVAKLVIGAIVLIGGVGLALWWVDKEQSKQP
                     SPAVKPADPWGIVNDSPIRPRR"
     gene            complement(4232..4537)
                     /locus_tag="PKX82_04790"
     CDS             complement(4232..4537)
                     /locus_tag="PKX82_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HOF51599.1"
                     /translation="MSACTTCGACCASFRVDFSVQEMQEMGGTVPAGLAVEVTASTCR
                     MRGTDHVPARCAALSGKLGDKIGCGIYEWRPSPCREFEEGSDACARARARHGLPELQ"
     gene            complement(4644..5618)
                     /gene="argF"
                     /locus_tag="PKX82_04795"
     CDS             complement(4644..5618)
                     /gene="argF"
                     /locus_tag="PKX82_04795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOF51600.1"
                     /translation="MSPNSKGIPSTLPKGAPPVRHYLQFDDFSADEYAYLLDRAAFIK
                     AKFKTFTRHHTLVDRTLAMIFEKASTRTRVSFEAGMYQLGGSVVHLTTGDSQLGRSEP
                     IEDSARVISRMTDIVMIRTYEQTKIERFAAHSRVPVINGLTNEFHPCQILADIFTYIE
                     HRGPIQGKTVAWVGDGNNMANTWLQAAALLGFHLRVSTPSGYELDEKVAVTPRGMGAG
                     SYKTYSNPMDACRGADLVTTDVWTSMGFEAENEARRKAFAAWCVNREMMSVAAPDALF
                     MHCLPAHRGEEVEAEVIDGPQSVVWDEAENRMHVQKALMEYLLLGRQE"
     gene            complement(5615..>6659)
                     /locus_tag="PKX82_04800"
     CDS             complement(5615..>6659)
                     /locus_tag="PKX82_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOF51601.1"
                     /translation="KLVPALQDQIAKLIHTSNYYHVPNQEVLAQMLVERSGMTNVFFC
                     STGLEANEAALKLARKFGHDKGIERPEIVVYEKAFHGRSIATLSATGNPKVQAGFGPL
                     VEGFIRVPINNIEQLKAATANNSNVVAVFFEAIQGEGGVNPMHMDYLRDVRALCDERD
                     WLLMIDEVQCGMGRTGKWFAHQWAGIKPDVMPLAKGLGSGVPIGAVVAGPRAANIFQP
                     GNHGTTFGGNPLATRAGVETIRIMEEDGLLANAAKVGAHLKTGLAAALQAEIASGGVK
                     EIRGMGLMLGIDLSKPCGALVQQAADKGLLISVTADSVVRLVPPLILSATEADQIVAI
                     LCPLISAFLKEAA"
BASE COUNT         1200 a         2012 c         2099 g         1348 t
ORIGIN      
        1 gattgcctcg gaccccatcg tatggcgtaa ggatctgccc gaagccacca agaccaagct
       61 caagaacttc ttcctgaact acgccaagac caatcctcag gaacgcgaag tcatggccaa
      121 gatgaactgg tccatgttca aggaatcgag caatgcccaa ttggtgccga tccgccagct
      181 ggatttcttc agcaagcgca acaagatcga gtcggacacc accctgagcg cagacgaccg
      241 caaactgcgc ctggccgaaa tcgacaagaa gctggctgaa ctgaaataac cgccacggca
      301 accgacacca tgcaagacgc agcctcttta agtacgcaga ccactgccct ggcggcggta
      361 cccaaacgca gcctggggtg gcatctttcc tgggccgtgc tgattggctt gctgcttgca
      421 tcgtggaagg gggctgacat gcgcccgctg gacctgtggc gtgactcggg caatatggcc
      481 acctacgtgg cggatttctt cccacccaac ttcagccagt ggcggatgta tgtggaagaa
      541 atggtggtta cgctgcagat tgctctctgg ggcactgctc tggccgttat caccgccatt
      601 cccatggcgc tgatggcctc ggccaatctg gtgccgtggt gggtgtacca gcccacacgg
      661 cggctcatgg atgccacgcg ggccatcaat gaaatggtgt ttgccatgct ctttgtggtg
      721 gccgtggggc tgggaccctt tgccggcgta ctggcgttgt ggattcacac caccggagtg
      781 ctcgccaaac ttttctccga ggcggtggag gcgatagatc cccagcccgt ggaaggcatt
      841 cgctctacgg gtgccagcgc gctgcatgaa atcatttacg gtgtgatccc gcaggtcatg
      901 cccttgtgga tttccttcac cctctaccgc tttgagagca acgtgcgctc tgcgtcggtg
      961 gtgggcatgg tgggagcagg cggcattggt gtggtgttgt gggaaatcat tcgcggtttt
     1021 cagtacgccg aaacctgtgc ggtgatgatc atcattgtgt tgaccgtgag catgattgat
     1081 gtggtatccg cccgcattcg aaaagtactg gtttgaagga gtacaggcat ggcactttcc
     1141 attgacgaca tcctccagct cttccagcga cgtgcccgca aacagtacgg ccgcgaggcc
     1201 gtgagccaga tggaacatgc gctgcagtgt gcgcagctgg cggaagaggc cggtgaaacg
     1261 tctgaaacca tcgtcgcgtc gctgttgcac gatgtgggcc acctgattgc ggcagagaac
     1321 gctgaccgtg ttgacgatac gcaggagcat gacgacctgc acgagtacat cgcgctgccg
     1381 tttctgcgca gcgtgctgcc cgatgcggtg ctggagccga tccgcctgca cgtggaggcc
     1441 aaacgctatt tgtgtatagc agatcccggc tactgggaca cactgtcgcc ggcatccaaa
     1501 cacagcctgg aacagcaggg cggcccgtat tcccaggatg ctgcggcggc gttcttgctg
     1561 cagccgtttg cacaggaagc cattcgcttg cgccggtacg atgactttgc caaagtgccg
     1621 cagaaagcga ctcccgacct ggcgtacttt gccacccgac tgcgcgcagt tgctctctga
     1681 tttagaggcc cgtgcgtaac gtccagatct ccgggaacag cacgacgtcg agcatcttgc
     1741 gcaagtagct cacaccaccg gtgccacccg ttccccgctt gaacccgatg acgcgttcca
     1801 ccgtggtcac atggcggaag cgccagaggc ggaaggcgtc ttccaggtct gtcagttctt
     1861 cacccagctg gtacagatcc cagtactttt gcggctcgcg gtacaccgtg agccaggcct
     1921 gctccaccgc atcgctggct tcatagggct gggtccagtc gcgctgggtg tggctctcag
     1981 gcaccgcaac gccacggcgc gccagcaggc gcagggcctc gtcgtacagc gagggggctg
     2041 catacgccgc ctgcaccagc gccaggcgtt cgggggcatg ggcgtgcggc ttgagcatgg
     2101 cggcattctt gttgcccagc gagaactcga tgcagcggta ctgaaagctc tgaaacccgc
     2161 tgctttgccc caggtaagga cggatcgcgc tgtactccgg cggcgtcatg gtggccagta
     2221 catcccaggc gtgcaccagc tgctccatga tcttgctcac ccgcgccagc atcttgaagg
     2281 ccgtacccaa ttcgtccctc gcgatgcact ggatagctgc gccgagttcg tgcagcatga
     2341 gtttcatcca cagctcgctg gtctggtgct gcacgatgaa cagcatctca tcgtgggccg
     2401 gcgagagcgg cttttgcgcg gacaggatgg tgtcgatctg caggtagtcg ccatagctca
     2461 tggatctgct gaagtcgagc agcgcttttt cgtcggcgac gattttttcg gtagaggtca
     2521 taagtggttc gagaggtaga cgggtggtac aggtttgggc cgatttgctg acccgcaggg
     2581 tctgatgagg cccgggcctg taccgcccgg ccgcctcgtg cgagaaccaa cctggagttc
     2641 aggtcactgc atgtttctgg ttgaactccg gctttttcca ttctgcggtt tccagaacct
     2701 ggcgcaggtg ctccaccgcg ttccacacgt cctcatagcc gatgtacagc ggcgtgaatc
     2761 caaagcgcag gatgtccttg tggatgccgc cgtcgccagc acggaagtcc ccaatcacgc
     2821 cgcgcgcaat cagggcctgc acgatggcaa aagcaccaga cccttctgcg ttgtgctcgc
     2881 gtgtcaggca cacctgcgaa ccgcgctgtg catggtcgcg tggtgtggcc aggcccagtc
     2941 cataaccctg acaacgctct tccaccagtt gaatgaacag gtcggtcagt gcaagcgact
     3001 tggtgcgcaa cgccgccatg ccacccagtg gagccgcggc ttcgaagcct tcgagtccgc
     3061 actccagcag tgacagactc acgatgggtt gcgtgccaca caggtagcgc gcaatgccgt
     3121 gtgccggctg gtagtcggcg gtaaacgcaa acggagatgc gtggccccac cagcccgaga
     3181 gcggttgcca gaagcggtcg gcatgtcgcg ggttgaccca cacaaaggcg ggggcgccag
     3241 ggccgccgtt gaggtacttg tagccgcagc caatcgagaa atcagcgcct gcgccgcgca
     3301 agtcgactgg cacggcacct gcgctgtggg caagatccca gatggtgagt gcgcctgcgg
     3361 cgtgggccgc cgcggttacg gcagccatgt cgtgcatggc gccggtgcgg tagttcacat
     3421 gggtcagcat cagcaccgcc acatcgcggg tgagagcgac ggcaatctct tcgggctcca
     3481 ccagctggag cgtaaagcca cgctccttgc acaagccctc ggcaatgtac aggtcggtgg
     3541 ggaagttgct gcgttcgctc acgatcacac gtttttcggg atggtcctgc gcagtaatgg
     3601 acagcgcggc ggacaagacc ttgaacaggt tgatggaagt gctgtcggtc acgacgactt
     3661 cgtcttgccc cgcgccaatc agcggggaga tgcggttgcc caggcgttgc ggcaggctga
     3721 accagcccgc ggtgttccag gaacggatca ggccctggcc ccactcctgg ttgaccactt
     3781 cggccgcacg tgccgcggct tttttgggca tcacgccaag ggagttcccg tccagataga
     3841 taacgccgtc gggaatggtg aattgattgc gcaggtggcg cagggggtca gcggcatcgc
     3901 gctgctggca gtcgtgtagc gtggtcattg cggtcctctt tgggggtggc cgcaaagtta
     3961 acactggtca aatttgtgtg gtgtcggtta tttccaacat gccggaaaaa tcctgccggg
     4021 aatcagcgcc gtggccggat cggtgaatca ttgacaatgc cccacggatc tgctggcttg
     4081 acggccggcg agggctgctt gctttgttcc ttgtccaccc accacagcgc cagacccacg
     4141 ccgccaatca ggacgatggc accaatcacg agcttggcta ccgtgagcca caggggcggt
     4201 tcgtcgttgt cgttctggct catggtggct cctattgcag ttctggcaga ccgtgccggg
     4261 cgcgggcacg ggcgcaggca tcgctgcctt cttcaaattc gcggcagggc gaaggccgcc
     4321 actcgtagat tccgcaccca atcttgtcgc ccagcttacc ggacagtgca gcgcaacgcg
     4381 cgggtacatg gtcggtgccg cgcatgcggc aggtgctggc ggtgacttca accgccagtc
     4441 cagcgggcac ggtgccaccc atttcctgca tttcctggac agaaaagtcc acccgaaaac
     4501 tggcacagca ggctccgcag gttgtgcagg cgctcatgag aatatggccc ccacgctcca
     4561 ccgctgtgcg ggtcgctgcc ccccacgggg gctcatcctg cttggggcgg cccggcgcag
     4621 gattgtggcc aggcggcgaa tgttcactct tgccggccca gcagcaggta ttccatgagc
     4681 gccttctgca catgcatgcg gttctccgct tcgtcccaca ccacgctttg cgggccatcg
     4741 atcacttcgg cttccacttc ctcgccgcgg tgggcgggca ggcagtgcat gaacagcgcg
     4801 tccggtgcgg ccacggacat catctcgcgg ttgacgcacc aggccgcaaa ggccttgcgg
     4861 cgggcttcgt tctcggcttc aaagcccatg ctggtccaca catcggtggt gaccagatcg
     4921 gcgccacggc aggcgtccat cggattgcta taagttttat agctgcctgc gcccattcca
     4981 cggggggtaa cggctacttt ctcatctaat tcatagccac tgggtgtgct gacgcgcaga
     5041 tggaaaccca gcagggccgc cgcttgcagc caggtgttgg ccatgttgtt gccgtccccc
     5101 acccaggcca cggttttacc ctggatcggg ccacggtgtt cgatgtaggt aaagatgtcg
     5161 gccaggatct ggcaggggtg gaattcgttg gtcaggccgt tgatgaccgg cacgcggctg
     5221 tgcgcagcga agcgttcgat cttggtctgc tcgtaggttc ggatcatcac gatgtcggtc
     5281 atgcggctga tgacacgggc gctgtcctcg atgggctccg agcgccccaa ctggctgtca
     5341 ccggtggtca ggtgcaccac gctgccgcca agctggtaca tgccagcctc gaagctgacg
     5401 cgtgtgcgcg tggatgcctt ttcgaaaatc atggccagtg tgcggtccac cagcgtgtgg
     5461 tggcgcgtga aggtcttgaa cttggccttg atgaaggccg cgcggtccag caggtaggcg
     5521 tattcgtcgg cactgaagtc gtcgaactgc aggtagtgtc gcaccggggg ggccccctta
     5581 ggcagggtgc tggggatacc tttgctgttg gggctcatgc ggcctccttc agaaacgcgg
     5641 aaatcagcgg gcacaggatg gccacgatct ggtcggcctc ggtagcgctc aggatcagcg
     5701 gcggcaccag gcgcaccacg ctgtcggcgg tcacgctgat cagcaggcct ttgtcggctg
     5761 cctgctgcac cagggcacca cagggtttcg agaggtcaat gcccagcatc aggcccatgc
     5821 cacgaatttc tttcacgccg ccactggcga tctcggcttg cagggcagca gccaggcccg
     5881 tcttgaggtg ggctcccact ttcgcggcgt tggccagcaa gccgtcttct tccatgatgc
     5941 ggatggtttc cactccggcg cgggtggcca gcgggttgcc accaaacgtg gtgccgtggt
     6001 tgccgggctg gaagatgttg gcagcacgtg ggccggctac cacggcgcca atcggcactc
     6061 ccgagcccaa gcccttggcc agaggcatga cgtccggctt gatacccgcc cattggtggg
     6121 caaaccactt gccggtgcgg cccatgccgc actgcacctc gtcaatcatc agtagccagt
     6181 cacgttcgtc gcacagggcg cgcacatcgc gcaggtagtc catgtgcatg gggttcacgc
     6241 caccttcgcc ctggatggct tcaaagaata ccgccaccac attggagttg ttggcggtag
     6301 cggccttgag ctgctcgata ttgttgatcg gcacgcggat gaaaccttcc accagggggc
     6361 caaaaccggc ctgcaccttg gggttgccgg tggcgctcaa tgtggcaatg ctgcggccat
     6421 ggaaagcctt ttcgtagacc acgatctcgg ggcgttcgat gcccttgtcg tgaccgaact
     6481 tgcgggccag cttgagcgcg gcttcgttgg cttccagtcc ggtggagcag aagaacacat
     6541 tggtcatgcc cgagcgttcc accagcatct gggccagcac ttcctggttg ggtacgtggt
     6601 agtaattgga ggtgtggatc agcttggcga tctggtcctg cagtgccggc accagtttg
//
LOCUS       DAOSJM010000095         6647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10478_length_6647_cov_3.579035, whole genome shotgun sequence.
ACCESSION   DAOSJM010000095 DAOSJM010000000
VERSION     DAOSJM010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10478_length_6647_cov_3.579035"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKX82_04805"
     CDS             <1..734
                     /locus_tag="PKX82_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOF51602.1"
                     /translation="DGRIPIVGVTGTQNTGRIARLVAWLVHISGKHVGLACSEGLYLD
                     GRQVVASDCAHWEAGQRLLINRSVQAAVFENPSRSILAEGLAYDKCNVGVVTDVGWHA
                     ELSEFDILDAEQTYKVARTQVDVVLPSGTAVLNAADEQVVEMAELCDGKVIFYGMDSG
                     LPAIAEHQQEGERVVFLRDGDIVLAQGSQDVAKLPLSSLKPSKASKPEMVMAAVAAAW
                     ALDIPIELIAAGLRTFESNPKKTPY"
     gene            742..3312
                     /gene="cphA"
                     /locus_tag="PKX82_04810"
     CDS             742..3312
                     /gene="cphA"
                     /locus_tag="PKX82_04810"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOF51603.1"
                     /translation="MEVTRVRALRGPNLWSHHTAIQSIVTCTAQEACVSKIDGFEARI
                     RARFPEISLFQPTGHDDSIPMAHVLELAALGLQAQAGCPVTFSRTTATLEPHVYQVVV
                     EYSEEEVGLLAMELAQALCQSAVDNTPFDLTSALTQLKDLDEDVRLGPSTGSIVDAAV
                     ARGIPYSRMTEGSMVRFGWGSKQRRIQAAEMDITSAISEAIAQDKQLTKKLLAAAGVP
                     VPGGRPVTDAEDAWKAACEIGLPVVVKPNDGNQGKGVTVNISDKEQLLKAYAAACEFR
                     DDILVERYMPGNDFRLLVVGDKLVAAARRDPPKVVGDGVHTITQLVEQVNKDPRRGSG
                     HSTSLTKIRFDDIARSCLASQGFEADSIPVKGQRVNLRNNANLSTGGSATDVTDDVHP
                     DVAARAVAAARMVGLDICGVDVVCDTILRPLEEQGGGMVEVNAAPGLRMHLAPSFGKG
                     RAVGEAIISSMFKPGQNGRIPIVAVTGTNGKTTTVRLISHLLTHNGMCVGMTNTDGVY
                     VGGTVIDTGDCSGPKSARSVLLHPDVDAAVLETARGGVLREGLAFDRCDVAVVTNVGS
                     GDHLGLNYITTVDELAVLKRVIVQNVSPKGTAVLNAVDPNVASMASKCKGSVIFFAAD
                     KFHPVMATHRAQGQAVVYVENGCVVAAKGDFHHSIPLSEIPVTLGGAIGFQVDNVMAS
                     VAAAWALGLDWRTIGNGLKSFSNDSHNAAGRFNLFQYRGATVIADYGHNPDAMLALVQ
                     AVEALPAKRRSVVISGAGDRRDQDIRQQTEILGNAFDEVILFEDQCQRGRQNGEVIAL
                     LREGLANAGRTKESSEFYGEFLAIDAAMDKLQDGDLCLILVDQVEEAMAHIAARVANS
                     "
     gene            complement(3325..4341)
                     /locus_tag="PKX82_04815"
     CDS             complement(3325..4341)
                     /locus_tag="PKX82_04815"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOF51604.1"
                     /translation="MKIIVLGAGAWGTALAISASRNPQGHQVTLWARDADQAQAMRTQ
                     RENARYLPGAPFPPALQIDCQPVSELVGSLAQHDLIVVGSPMSGLRGLLTSLEDCSIP
                     VAWLCKGFEAARDASYGLLGHEVQAQVAPHMQAGVLSGPSFAQEVAQGKPTALVAASA
                     HAQVREALVQAFHSPTLRVYASDDVVGVEVGGAVKNVLAIATGLCDGLQLGLNARAAL
                     VTRGLTEMTRLGLALGARADTFMGLSGLGDLVLTSTGDLSRNRRVGLALAQGKSLQQA
                     VDELGHVAEGVYCARTVVQRALGLGVDMPIARCVVELLDGRMQPAQAVAALMGRDPTV
                     EMAS"
     gene            complement(4390..4836)
                     /gene="secB"
                     /locus_tag="PKX82_04820"
     CDS             complement(4390..4836)
                     /gene="secB"
                     /locus_tag="PKX82_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOF51605.1"
                     /translation="MADQEPVFQIQRVYLKEASLEQPNSPAILLEQEQPSVDIQLGVE
                     AAPVADGVFEVSVTATVQTKIKDKTVFLVEAKQAGIFEIRNLPEEQLGQIMGIACPQI
                     VYPYLRGNVADLIQRAGFPPVHLSEINFQAMYEQQKAEAAGAGLPQ"
     gene            complement(4922..5182)
                     /gene="grxC"
                     /locus_tag="PKX82_04825"
     CDS             complement(4922..5182)
                     /gene="grxC"
                     /locus_tag="PKX82_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HOF51606.1"
                     /translation="MQTVKMYTTAVCPYCVQAKRVLKSKGVENIEEVRVDANPDERMK
                     MMELTGRRTVPQIFIGSTHVGGCDDLMALDSKGGLVPLLNAA"
     gene            complement(5187..5594)
                     /locus_tag="PKX82_04830"
     CDS             complement(5187..5594)
                     /locus_tag="PKX82_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOF51607.1"
                     /translation="MKFIVDNWMLITIAITSGGLLLWPVLQGAATVGLNPTAAVQLMN
                     REKAVVIDVCEPGEHAAGHIVGAKNIPMGQLEAKLAGVVKNKATPILMVCQSGARSAR
                     SVAIAKKLGYEQAQSLAGGIGAWKSANLPVEKG"
     gene            5709..6452
                     /gene="gpmA"
                     /locus_tag="PKX82_04835"
     CDS             5709..6452
                     /gene="gpmA"
                     /locus_tag="PKX82_04835"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOF51608.1"
                     /translation="MYKLVLIRHGESTWNLENRFTGWTDVDLTPTGVSQAMSAGKLLK
                     AEGYAFDLAYTSVLKRAIHTLWYALDEMDCTWLPVVKNWRLNERHYGALQGLNKADMA
                     KQYGDDQVLIWRRSYDTPPPALEANDPRCERGDRRYANMQAADVPLTECLKDTVARVI
                     PFWNESMAPAIKAGKRIVVAAHGNSIRALVKYLDNIADDAIVGVNIPNGIPLVYELDE
                     NLKPIRHYYLGDAEAAAKAAAAVASQGKA"
BASE COUNT         1326 a         2128 c         1982 g         1211 t
ORIGIN      
        1 aggacggccg tatcccgatt gtgggcgtca ccggcaccca gaacaccggg cgcattgcgc
       61 gtctggtggc ctggctggta cacatcagcg gcaagcacgt aggcctggcc tgcagtgaag
      121 gcctgtacct ggacggacga caggtggtcg ccagcgactg cgcccactgg gaagccggcc
      181 agcgtctgct gatcaaccgg tcggtgcagg cagcggtgtt tgaaaacccc agtcgcagca
      241 tcctggccga aggtctggcc tacgacaaat gcaacgtcgg cgtcgtcacc gatgtggggt
      301 ggcacgctga gctgtcggaa ttcgacattc tggatgccga gcaaacctac aaggttgcgc
      361 gcacccaggt ggatgtggtg ctgcccagcg gcaccgccgt cctcaatgca gcggacgaac
      421 aggtcgtgga aatggccgaa ttgtgcgatg gcaaggtcat cttctacggt atggacagcg
      481 gcctgcctgc cattgcggaa caccagcagg agggtgaacg cgtcgttttc ctgcgcgatg
      541 gcgacattgt tctggcacaa ggatcgcagg atgtggccaa gctgccgctg tcgagcctca
      601 agcccagcaa agcgtccaaa cccgaaatgg tgatggcggc tgttgcagcc gcctgggcgc
      661 tggacatccc gattgaactg attgcggccg gcttgcgtac tttcgagtcg aatccgaaga
      721 aaacccctta ctgagatccc catggaagtc actcgcgtcc gggccctgcg tggccccaac
      781 ctgtggagtc accacacggc catccagtcc atcgtcacct gcacggcaca agaggcctgc
      841 gtcagcaaga tcgatggatt cgaagcccgc atccgtgcac ggtttccgga aatcagcctc
      901 ttccagccca ctggccacga cgattccatc ccgatggccc atgtgctgga actggcagct
      961 ttgggattgc aggcgcaggc cggatgcccg gtcacattca gccggaccac cgccacgctc
     1021 gaaccccatg tctaccaggt ggtcgtggaa tacagcgaag aagaagtcgg cctgctggcc
     1081 atggagctgg cacaggccct gtgtcaatcg gccgtggaca acacgccttt tgacctgaca
     1141 agcgccctca cccaattgaa agacctggac gaagacgtcc gcctggggcc tagcaccggg
     1201 tccatcgtgg acgctgccgt ggcgcggggc attccctaca gccgcatgac cgaaggcagc
     1261 atggtgcgct ttggctgggg cagcaagcag cgccgcattc aggcagcaga gatggacatc
     1321 accagcgcca tttcggaagc cattgcgcag gacaagcagc tcaccaagaa actgctggca
     1381 gcagccggtg tgccggtacc cggtggccgc ccggtcacgg atgcagagga cgcctggaaa
     1441 gccgcctgcg aaatcggctt gccggtcgtg gtcaaaccca acgacggaaa ccagggcaaa
     1501 ggcgtcaccg tcaatatcag cgacaaagag caactgctga aggcctacgc cgccgcctgc
     1561 gaattccgcg atgacatcct ggtagagcgc tatatgccag gcaacgattt tcgtttgctg
     1621 gtagtcggcg ataagctggt tgcggcggcc cgacgtgacc cgcccaaggt cgtgggtgat
     1681 ggcgtgcaca ccatcacgca gctggtggag caggtcaaca aggacccccg ccgtggtagc
     1741 gggcactcca cgtccctcac caaaatccgt tttgacgaca ttgcacgatc ctgcctcgct
     1801 tcgcagggat ttgaagccga ctcgattccc gtcaagggac aacgcgtcaa tctgcgcaac
     1861 aacgccaacc tgtccaccgg cggctcggcc accgatgtca ccgacgatgt gcaccccgat
     1921 gtagccgcgc gtgccgtggc cgcagcgcgc atggtagggc tggatatctg cggtgtggac
     1981 gtggtgtgcg acaccatctt gcgcccactg gaagaacagg gcggcggcat ggtggaagtc
     2041 aacgccgcac ccggcttgcg catgcatctg gcaccctcgt ttggcaaagg ccgcgctgtg
     2101 ggcgaggcca tcatttccag catgttcaaa cctggccaga acggtcgtat tccgatcgtg
     2161 gccgtcactg gcaccaatgg caaaaccacc acggtccgtc tgatttcgca cctgctgacc
     2221 cacaacggca tgtgtgtcgg catgaccaat accgatggtg tctatgtcgg cggaaccgtg
     2281 atcgacactg gcgactgcag cggtcccaag agtgcacgca gcgtgctgct gcacccggac
     2341 gtggatgccg ccgtgttgga aaccgcccgc ggcggtgtgt tgcgcgaggg cctggccttt
     2401 gaccgctgcg atgtggcagt ggtcaccaac gtcggcagtg gcgaccatct gggcctgaac
     2461 tacatcacca ccgtggatga acttgcggtg ctcaagcgcg tgatcgtgca gaacgtatcg
     2521 cccaaaggta cggctgtgct caatgcagtt gaccccaacg tggcgtccat ggcaagcaag
     2581 tgcaagggct cggtcatctt ctttgccgcc gacaagtttc accccgtcat ggcgacacac
     2641 cgggcgcagg ggcaggccgt ggtgtacgtg gaaaacggat gtgtggtcgc tgccaaaggc
     2701 gactttcatc acagcatccc cctctcggaa attccggtca ccctgggcgg cgccatcggt
     2761 ttccaggtgg acaacgtgat ggcctcggtg gctgcagcct gggcgctggg tctggactgg
     2821 cgcaccatcg gcaatggcct gaagtcgttc tccaacgaca gccacaacgc ggcgggtcgc
     2881 ttcaacctct tccagtaccg tggagccacc gtgattgccg actacggcca caacccggat
     2941 gccatgctgg cgctggtgca agcggtcgag gcattgccgg ccaaacgccg ctcggtggtt
     3001 atcagtggcg ccggcgaccg gcgtgaccag gacattcgcc agcagaccga gatcctcggc
     3061 aacgcgttcg acgaagtcat tttgtttgaa gaccagtgcc agcgtggacg ccagaatggc
     3121 gaagtgatcg cgctgttgcg cgagggcctg gccaacgccg gccgcacgaa agagtccagc
     3181 gagttctatg gcgagtttct ggcgattgac gccgccatgg acaagctgca ggacggcgat
     3241 ctgtgcctga tcctggtaga ccaggtggaa gaggccatgg cgcacatcgc tgcacgcgtg
     3301 gccaacagct agggcaaggc taccctagct ggccatttca actgtagggt cccggcccat
     3361 cagggccgcc acggcctgcg ccggttgcat gcggccatcg agcaattcca ccacacagcg
     3421 ggcgatcggc atgtccacgc ccaggcccaa ggcacgctgc accaccgtgc gggcgcaata
     3481 gacaccctcg gccacgtgtc ccagttcatc taccgcctgc tgcaggctct tgccctgcgc
     3541 cagggccaga ccgacacggc ggttacgcga cagatcaccg gtggacgtca ggaccagatc
     3601 acccaagcca ctgaggccca tgaacgtgtc tgcgcgcgcg cccagggcca gccccagacg
     3661 cgtcatctcg gtcagaccgc gggtcaccag ggccgcacgg gcattgagcc ccaattgcag
     3721 gccgtcacac agcccggtgg cgatcgccag cacgttcttg acggcgccac ccacttccac
     3781 gccgaccaca tcgtcgctgg cgtacacgcg caaggtgggg ctgtggaagg cctgcaccag
     3841 ggcctcgcgg acctgcgcat gcgcgctggc agccaccagt gcggtcggct tgccctgcgc
     3901 cacttcctgc gcaaaactgg gaccactcag aaccccggcc tgcatgtgcg gtgccacctg
     3961 cgcctgcact tcatggccca gcagtccgta ggatgcatcg cgcgccgcct cgaaaccttt
     4021 gcacagccag gccacaggga tgctgcagtc ctccagactg gtcagcagcc cgcgcaatcc
     4081 cgacatgggc gagcccacca cgatcagatc atgttgggcc aggctgccca ccagttccga
     4141 tacaggctgg cagtcgatct gcagggctgg aggaaatggc gctcccggca ggtagcgtgc
     4201 gttctcgcgc tgggtgcgca tggcctgagc ctgatcagca tcccgggccc acagggtcac
     4261 ctggtgtccc tggggattgc ggctggcact gatggccagc gccgtacccc aggcaccggc
     4321 accaagcact attattttca tagctactta cgcaatatcc acgggggcta gcgcctgaaa
     4381 acgcttcaat tactggggca aaccggcacc agccgcttca gccttctgct gttcgtacat
     4441 ggcctggaaa ttgatttccg acaggtgcac gggcgggaag cctgcgcgct ggatcaggtc
     4501 ggctacgttg ccacgcaggt aggggtacac gatttgcgga caggcaatgc ccatgatctg
     4561 gcccagctgc tcttctggca ggttacggat ctcgaagatg cccgcctgtt tggcttccac
     4621 cagaaacacg gtcttgtcct tgatcttggt ttgcactgtg gcggtcaccg acacttcaaa
     4681 cacgccgtca gccaccggcg cggcttccac gccgagctgg atgtccacgg aaggctgctc
     4741 ctgctccagc aggatggcgg gggaattggg ctgctccagc gaagcctcct tgaggtacac
     4801 gcgctgaatc tggaatacgg gttcttggtc tgccatggca acagtctttc aaagtaaaac
     4861 aaagcccgcc cggacacagc ctggcgggcc aatcaagcaa attgctctaa cgaggattat
     4921 ctcaggctgc gttgagcagc ggcaccagac cgcccttgct gtccagcgcc atcaggtcat
     4981 cgcagccacc cacatgggtg gatccgataa aaatctgcgg cacggtgcgt cgcccggtca
     5041 gttccatcat cttcatgcgc tcatcaggat tggcatccac gcgcacttct tcaatgttct
     5101 ctacgccttt ggacttgagg acgcgcttgg cctgcacgca ataggggcag acagcggtgg
     5161 tgtacatctt gacggtttgc atggatttac cctttctcta ccggcagatt agcgcttttc
     5221 cacgcaccga ttccgccggc cagtgattgg gcctgctcgt aacccagttt cttggcaatg
     5281 gccacagacc gcgcagaacg cgcgccggac tggcacacca tcagaatggg ggttgccttg
     5341 ttcttgacga cgcccgccag cttggcttcc agttgcccca tcgggatgtt cttcgcaccc
     5401 acaatgtggc ccgcagcgtg ttcgccgggc tcacagacat caatcaccac ggctttttcg
     5461 cggttcatca gctgcactgc cgcggtgggg ttcaatccca ccgtggcagc accctgcagt
     5521 acgggccaca gcaaaaggcc tccagatgtg atggcaatcg tgatcagcat ccagttatcg
     5581 acaataaatt tcactttggt ccttgttggt tacacgaccg ttacccaacg gtcagcccgt
     5641 cattttagaa tgttgggcta accaccgtcc gccagggcgg ttattcccca cgcaccggcc
     5701 tgttgaccat gtacaaactc gttctcattc gccatggcga atccacctgg aatcttgaaa
     5761 accgcttcac cggctggacc gatgtggacc tgacgcccac cggcgtgtcc caggccatgt
     5821 ccgctggcaa actgctcaag gcagagggtt acgcatttga tctggcctac accagcgtac
     5881 tcaagcgtgc catccacacc ctgtggtacg ccctggacga aatggactgc acctggttgc
     5941 ccgtggtcaa gaactggcgc ctcaacgagc gccactacgg tgccctgcag ggcttgaaca
     6001 aggccgacat ggctaagcaa tacggtgatg accaggttct gatctggcgc cgcagctacg
     6061 acacccctcc gcccgccctg gaagcgaacg acccccgctg cgagcgcggc gaccgccgct
     6121 acgccaacat gcaggccgcg gacgtgccac tgaccgaatg cctgaaggac accgtcgccc
     6181 gcgtgattcc gttctggaac gaatccatgg ccccggccat caaggcaggc aagcgtattg
     6241 tggtggcggc ccatggcaat tccatccgcg cactggtgaa atacctcgac aacatcgccg
     6301 acgatgccat cgtgggggtc aacatcccca acggcattcc gctggtgtat gaactggacg
     6361 aaaacctcaa gcccattcgc cattactacc tgggcgatgc agaagctgcc gccaaggccg
     6421 cagctgccgt tgcctcacag ggtaaagcct gagcaaagta aggccgcatc aggggaactg
     6481 actgcggcca accgcctcca aggtgtatat tgagacggca caggaataca tatgggtcac
     6541 aaactgaaaa ttaccggttg ggttgctttg ggcgtcgtcg ccggggcgct caccaccgtg
     6601 tcactgcaaa ccgttgcacg caacacgctg tctcccctcc ccctgga
//
LOCUS       DAOSJM010000096         6616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10536_length_6616_cov_4.599299, whole genome shotgun sequence.
ACCESSION   DAOSJM010000096 DAOSJM010000000
VERSION     DAOSJM010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10536_length_6616_cov_4.599299"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PKX82_04840"
     CDS             complement(<1..1024)
                     /locus_tag="PKX82_04840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF51609.1"
                     /translation="MTDPLHISEVDAATARQNGFVQWVYALRRTLVTTPVGWLLVAWF
                     GRQTVPHDLIFMWLGVFAAVWAGGLWVLWGVIRQGPSLVRHRTRTFWVAGSDGMAWGL
                     MAWFLMGHDPIHDATLTAMLCGVIAINAQVYVTFIRAYYVMVGVLWLVFITGMLRIAD
                     HPHTIDYAAGLSVFVGLTLYYMRNIAKRVAQGIALQLANVSLTEQLRIALQMVRLDAA
                     TDALTGQGNRRALDELLKQQMEFAAQTGRTFSILMLDIDFFKKINDVHGHMVGDDALR
                     SFAQRVREHLRLGDVCARFGGEEFVAVLPGASREEALEVAERLRSGVEAEPLLTTPLV
                     AVTVSIG"
     gene            complement(1153..2547)
                     /locus_tag="PKX82_04845"
     CDS             complement(1153..2547)
                     /locus_tag="PKX82_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51610.1"
                     /translation="MTIQAKVTTPADDSPPLQSRAVVVLTGLSYLFTGFGFSFLDRRF
                     GSFGLEGFLWLVWAVLGFGAGALHVGRSAAGKTQWLVLGALGGLLALFPGFAIYNLLR
                     WASLTLMIVMGARAAVLKTRRDFYFTLIVIFVVGFMVGTHGNADWTLWFYVGPAWVFG
                     GLALAWDHAAGTPLSRWTKMFMTLGFISASFLLAVALFFFAPRPPVLGFGFLPPGTDT
                     PGMFTQPAGSGGQQGKGGGNGGAGSGGSAGQGSAGAPQQGSWGQQWDAMLQGMRRAAS
                     DKTIPQWQRSIMGKALDWAQILHGLLTGRAFKPAGDPVQMDAPPQPLEQEEMFSFRVN
                     WLLVLALLLLGYLLWRRRYRLGLQLVLGSAWLLAGRYPAQSMRLSAQAMKWCLHMQGH
                     KRRPGQSVREHWVAAAGVAPLARRWLGYAVESYCAMRFGNVPATPQQAIHMRKAVMGA
                     CDIMLGAVPELATA"
     gene            complement(2544..3710)
                     /locus_tag="PKX82_04850"
     CDS             complement(2544..3710)
                     /locus_tag="PKX82_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOF51611.1"
                     /translation="MERWLSKSRVLLGLCAVLLMAALNRQDPMVYAMFLFLGVVSVLG
                     FLLPWLSLRSTTVRLDGAANIEVMEGADCNLNILVERTAPWPAFMVDIETEWEWASQR
                     IVLSQTVPVIRAGRAADLGRLIQFPCRGRYELIAVRLSSGFPLGLVRARHTLSRPQIH
                     LRVLPAAQPVQWPLPWDITEDPQGELTTRRMGQSFELGMLRPYQHGDSVGRVSWRASA
                     RVGELVIQHFQQSGSVRLRVAVEVPHGTSLGDATGAGEQAIRLAAGVCHAALNNSAQL
                     FLYLEHQTEPVRDASAILPALAETEPSGNGFFPVLARVTHATAPGEQVAVVVSSTCPG
                     PELVLALGALAAQGCRVVVCIALGRRKVPLELAQAKALQKALQQAGFATLMEAP"
     gene            complement(3719..4681)
                     /locus_tag="PKX82_04855"
     CDS             complement(3719..4681)
                     /locus_tag="PKX82_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOF51612.1"
                     /translation="MSQTQATAASARDSAQLAQTIVRNVEKVLEGKHQQVELALAAIV
                     AGGHLLIEDVPGLGKTMLARALAVSVGLSFKRIQFTADLMPSDIVGGPVYNPKDGSFV
                     LRPGPVFANIVLADEINRANPRAQSALLECMEEGQITLDGQTIPLPAPFAVLATQNPI
                     DMAGTYPLPEAQLDRFLIRLSLGYPDAQTEAGLITTQQFSHPISRLQPVCATGDFARL
                     VASARDVTVTPEVAAFMTSIVAATRKHASIRFGASPRGTLGLARMARALSCVRGLSYV
                     DPAVVREVAVPVLAHRIIVQTQGTQPKPAEALIQDILQSQHTPQ"
     gene            4771..5019
                     /locus_tag="PKX82_04860"
     CDS             4771..5019
                     /locus_tag="PKX82_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51613.1"
                     /translation="MKKLNITISLEMSVPEDWELVETSEGTPVLKLPNGQFMDLAIEP
                     LFATDPEETWSSTDDEDTLNDILDMVESEEVAYEFVTH"
     gene            5019..6266
                     /locus_tag="PKX82_04865"
     CDS             5019..6266
                     /locus_tag="PKX82_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOF51614.1"
                     /translation="MTSAQWFLLIGALLLVRGLTATLLERSPVTSAIVYLGVGLLVGP
                     SALNLFHFNPLKESALLEALTEVAVLISLFSAGVKMPGTFTFARWRAPVLLATASMAL
                     TVGLVAAFAVWVLDLPLGAGILLGAILAPTDPVLATDVQIRHPGDRDQLRFTLTCEAG
                     MNDGSAFPFVMLGLGLLGLHNLGDWGTRWVVVEVLWATVGAIAIGVVAGIALARLGWM
                     LRRGPKHHALLDDFLGLGLIGVVYGLCVMVDAWGFLAVFFAAVALRQTEQKLARTALP
                     HAEPGSLPLVSEGSLVFKEHLERLSEMVLVILLGGTLFLNSWSWAAVGLSLFLFLVAR
                     PVSVMLGTWGSGTPLRIRGLAAWFGVRGVGSLYYLMYAIQHGLPEPLALQLIQLTLIV
                     VTMSILVHGTSVKPLLALFWRRS"
     gene            complement(6263..>6616)
                     /locus_tag="PKX82_04870"
     CDS             complement(6263..>6616)
                     /locus_tag="PKX82_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOF51615.1"
                     /translation="PYSFANNTSGCWVNGGIHGPTYEEPKTTWLPNSTAPAGLMFYTG
                     TRFTDMGWQGSAFTGGLAGSTLWRIVFSGSNVASKEEITVIKNLGQRIRAVQQGPDGW
                     IYLLTDAGQLIRLER"
BASE COUNT         1237 a         2128 c         2136 g         1115 t
ORIGIN      
        1 cgccaatgga cacggtgacg gctaccagag gcgtggtcag cagtggctcc gcctcgaccc
       61 cgctacgcag gcgctcggcc acttccaggg cttcctctcg cgaggcacca ggcagcacag
      121 ccacaaactc ctcgccgcca aagcgggcgc atacgtcccc caggcgaagg tgctcgcgca
      181 cacgctgggc aaaggaacgc aaggcatcgt cgcccaccat gtggccgtga acgtcgttga
      241 tcttcttgaa aaaatcgatg tcgagcatca ggatggaaaa ggtgcgcccg gtctgggcgg
      301 caaattccat ctgctgcttg agcagttcat ccagtgcacg gcggttgccc tggccggtga
      361 gcgcatcggt ggcggcgtcc aggcgcacca tttgcagggc aatacgcagt tgctcggtca
      421 gcgagacatt ggccagctgc aacgcaatgc cctgcgccac gcgcttggca atgttgcgca
      481 tgtagtacag cgtgagcccc acaaagacgg acagccccgc cgcatagtcg atggtgtggg
      541 gatggtccgc aatacgcagc atcccggtaa tgaagaccag ccacaaaaca ccgaccatca
      601 cgtagtacgc gcggatgaag gtcacataca cctgcgcatt gatggcaatg acaccgcaca
      661 gcatcgcggt cagcgtcgca tcgtggatgg ggtcatggcc catgaggaac caggccatca
      721 aaccccaggc cattccgtcc gagccagcca cccagaatgt gcgcgtgcga tggcgcacca
      781 ggctgggccc ttggcgaatc acgccccaca gcacccacaa gccaccagcc cagaccgcgg
      841 caaaaactcc caaccacatg aaaatcaggt cgtggggtac cgtctgcctg ccaaaccagg
      901 ccaccagcag ccagcccacg ggcgtggtga ccagcgtgcg acgcagggcg tacacccatt
      961 gcacaaaccc gttctggcgc gcagtcgcgg cgtcgacttc agagatatgc aaagggtccg
     1021 tcatggggca acaggggtgt ctcgttttat ttgctactct attgatagca atatgcgcaa
     1081 attctacgcg ggccgaggcc agattgtcta tatgacgctg tcggtgaaag ggttatcgaa
     1141 cagctgccca aactacgccg tagccagctc aggcacagcg cccagcatga tgtcgcacgc
     1201 gcccataacg gccttgcgca tatggatagc ctgctgcggt gtggccggca cattgccaaa
     1261 acgcatggcg cagtaggact ccaccgcata gcccagccag cgtctggcca gcggtgcaac
     1321 gcctgccgcg gccacccagt gctcgcgcac cgactggccc gggcgccgct tgtggccctg
     1381 catgtgcagg caccatttca tggcctgcgc cgacaggcgc atggactgcg caggatagcg
     1441 cccggccagc agccacgcgc tgcccagcac cagctgcagg cccaggcggt acctgcggcg
     1501 ccacagcagg tagcccaaca gcagcagcgc cagcaccaga agccagttca cgcggaagga
     1561 aaacatttct tcctgctcca gcggctgtgg gggcgcgtcc atctgcacgg ggtcaccagc
     1621 gggcttgaag gcccgcccgg tcagcagccc gtgcaggatt tgggcccagt cgagtgcctt
     1681 gcccatgatg ctgcgctgcc actgtggaat ggttttgtcc gatgcggccc tgcgcatgcc
     1741 ctggagcatg gcatcccact gctggcccca gctgccttgc tgcggcgcgc cagcactacc
     1801 ctgcccggcg ctgccaccgg agcccgctcc gccattgccg ccacccttgc cctgctggcc
     1861 gccgctacct gcgggctgtg tgaacatgcc cggcgtgtcg gtacccggtg gcaggaaacc
     1921 aaagcccagc accggtggcc ggggggcgaa gaagaacagc gccactgcca gcagaaacga
     1981 ggcgctgatg aaccccagcg tcatgaacat cttggtccag cgcgatagtg gggtgcccgc
     2041 tgcgtggtcc cacgccaggg caagcccgcc aaacacccag gccggcccaa cgtaaaacca
     2101 caaagtccag tccgcgttgc catgggtgcc caccatgaag cccaccacaa aaatgacgat
     2161 cagcgtgaag tagaagtcac gccgcgtctt gagcacggcg gcacgcgccc ccatgacaat
     2221 catcagcgtc agcgaagccc agcgcagcag gttgtaaatg gcaaagcccg gaaacagcgc
     2281 cagcagcccc cccagggccc ccagcaccag ccactgggtt ttgccagcag cagacctgcc
     2341 cacatggagc gcgccagcac caaaccccaa gacggcccac accagccaga ggaaaccctc
     2401 cagcccaaag gaaccaaagc ggcggtccag aaacgaaaaa ccgaatccgg taaacaggta
     2461 actcaacccg gtgagcacca ccaccgcccg gctctgcagc ggtggtgagt cgtcggcagg
     2521 cgtggtgact ttggcctgaa tggtcatggc gcctccatga gcgtggcaaa gccggcctgc
     2581 tgcagggcct tttgcaaggc cttggcctgc gcaagctcca gcggcacctt gcggcgcccc
     2641 aatgcaatgc agaccacaac ccggcagccc tgtgcagcca aagcccccag cgcaagcacc
     2701 agttctggcc ccgggcaggt gctgctgacc accacggcca cctgctcgcc cggcgccgtg
     2761 gcgtgggtca cccgcgccag caccggaaag aacccgttgc ccgaaggctc cgtctctgcc
     2821 agtgcgggca ggatggcgga cgcatcgcgc acaggctcgg tctgatgctc caggtagaga
     2881 aacaattgcg cgctgttatt gagcgcggca tggcacacgc cggcagccag gcgaatggcc
     2941 tgctcacctg cacccgtggc gtcccccagg ctggtgccat ggggcacttc caccgccacg
     3001 cgcagacgca cggagccgct ttgctggaaa tgctggatca ccagctcccc cacccgggcc
     3061 gaggcacgcc agctgaccct gccgacggaa tcgccatgct ggtagggacg cagcatgccc
     3121 agctcgaacg actggcccat gcggcgggtg gtcagctcgc cttgcgggtc ttcggtgatg
     3181 tcccacggca ggggccactg cactggctgc gctgcgggca acacacgcag gtggatttgc
     3241 ggccgggaca gcgtgtggcg ggcgcggacc agccccaacg gaaagccgct ggacaggcgc
     3301 acggcaatca gctcgtaacg cccgcggcag ggaaactgga tcaggcgccc caggtccgcc
     3361 gcgcggcctg cgcgaatcac cggcacggtc tggctcagca cgatgcgctg cgaggcccac
     3421 tcccactcgg tttctatgtc caccatgaag gcgggccacg gtgccgtgcg ctccaccaga
     3481 atgttgaggt tgcaatccgc accttccatg acttcaatgt tggccgcacc atccagccgc
     3541 accgtggtgg agcgcagcga aagccagggc agcagaaagc ccagcacact gaccaccccg
     3601 aggaacagga acatggcata caccatgggg tcctggcggt tgagcgcggc catgagcagc
     3661 actgcgcaca ggccgagcag cacccgggac ttggacagcc agcgctccat ggatgggcct
     3721 actgcggtgt gtgctgtgac tgcaggatgt cctgaatcag cgcctcagcc ggcttgggct
     3781 gcgtgccctg ggtctgcacg atgatgcggt gcgccagcac aggcaccgcc acttcgcgca
     3841 ccacggccgg gtccacatac gagagcccgc gaacgcagct cagggcgcgt gccatgcggg
     3901 ccagacccag ggtgccgcgc gggctggcac cgaaacggat ggatgcatgt ttgcgcgtgg
     3961 ctgccacgat gctggtcatg aaggcggcca cttcgggggt gacggtgaca tcgcgcgcgc
     4021 tggccaccag gcgcgcaaag tcgccggtgg cacacaccgg ctgcaggcgg ctgatggggt
     4081 gcgagaactg ctgggtggtg atgagcccgg cttcggtctg cgcatcgggg tagcccaggg
     4141 agaggcgaat gagaaagcgg tccagctgcg cttcgggcag cgggtaggta ccggccatgt
     4201 caatcgggtt ttgggtggcc aatacggcaa agggtgccgg cagcgggatg gtctggccat
     4261 cgagggtgat ctgcccttct tccatgcact ccagcagggc cgattgggcg cgcgggttgg
     4321 cgcggttgat ttcatcggcc agcacgatgt tggcaaacac cgggccgggg cgcagcacaa
     4381 agctgccgtc tttggggttg tagaccgggc caccgacaat gtcggacggc atcaggtcag
     4441 ccgtgaactg gatgcgcttg aacgacaggc ccaccgacac ggccagcgca cgggccaaca
     4501 tggtcttgcc caggccgggc acatcctcaa tcaacagatg cccgccagcc acgatggctg
     4561 ccagtgccaa ttcgacctgc tggtgcttgc cctccaggac tttctcaaca ttgcgaacaa
     4621 tggtttgcgc gagttgtgca gagtccctgg cggacgccgc agtggcttgg gtttggctca
     4681 attggtgttc tccctgtatc gggaaattct aatgggcgat aaagacacaa tttggatgta
     4741 ccctctgcac cctcacacaa ggaaacgacc atgaaaaaac tcaatatcac aatctcgctg
     4801 gaaatgtcgg tccccgagga ctgggaactg gtggaaacgt ccgaaggcac gccggtgctg
     4861 aagctgccca acggtcaatt catggacctg gcgatcgagc cgctgtttgc caccgacccc
     4921 gaggaaacct ggtccagcac cgacgacgaa gacacgctca atgacattct cgacatggtc
     4981 gagagcgagg aagtggccta cgagttcgtc acccactgat gacgagtgcg cagtggttcc
     5041 tgctgattgg cgcgctgctg ctggtgcggg gcctcacggc cacgctgctg gaacgatcgc
     5101 cagtcacctc ggccatcgtt tacctcgggg taggcctgct ggtgggcccg agtgcgctga
     5161 acctgtttca cttcaacccg ctcaaggagt cggcgctgct ggaagcgttg accgaggtgg
     5221 ccgtgctgat ctcgctgttc tcggcgggcg tcaagatgcc aggcaccttc accttcgcac
     5281 gctggcgtgc gccggtgctg ctggccacgg cgtcgatggc gttgacggtg ggcctggtgg
     5341 cggcgttcgc ggtgtgggtg ctggacctgc cgctgggcgc gggcatcttg ctaggcgcga
     5401 ttctggcgcc gaccgacccg gtgctggcca ccgatgtgca gattcgccac ccgggtgacc
     5461 gcgaccagct gcgctttacg ctgacctgcg aagcgggcat gaacgacggc agcgcattcc
     5521 cgtttgtgat gctgggcctg ggcctgctgg ggctgcacaa cctcggtgac tggggcacgc
     5581 gctgggtggt ggtggaagtg ctgtgggcca cggtgggcgc gattgccatt ggcgtggttg
     5641 cgggcattgc attggcccgg cttggctgga tgctgcgccg ggggcccaag caccatgcgc
     5701 tgctggacga ttttctgggc ctggggttga ttggcgtggt ctatggcctg tgcgtgatgg
     5761 tggacgcctg gggatttctg gccgtttttt ttgccgctgt ggcgctgcgc cagaccgagc
     5821 agaaactggc tcgcacagcc ctcccccatg ccgagccagg ttcgcttcct ctggtcagtg
     5881 aaggttcgct ggttttcaag gaacatctgg agcggctctc ggaaatggtg ctggtcatcc
     5941 tgctgggcgg cacgctgttt ctcaactcct ggagttgggc tgccgtgggc ttgagcctgt
     6001 tcctgtttct ggtggcgcgc ccggtgagtg tgatgctggg cacctggggc tccggcacgc
     6061 ccctgcgcat ccgcgggctg gcagcgtggt ttggcgtgcg cggggtgggc tcgctctatt
     6121 acctgatgta cgccatccag cacgggctgc ccgagccgct ggcattgcag ctgatccagc
     6181 tgacgctgat tgtggtgacc atgtcgatcc tggtgcacgg caccagcgtc aagccgctgc
     6241 tggcgttgtt ctggcggcgg agctagcgct ccagccggat cagctggccc gcatcggtca
     6301 gcaggtaaat ccagccgtcg ggcccctgct gaactgcgcg aatgcgctgg ccgaggtttt
     6361 tgatgacggt gatctcttcc ttggaagcca cattgctgcc actgaagacg atgcgccata
     6421 gggtggaacc cgcgagacca ccggtgaacg cactgccctg ccagcccatg tcggtgaacc
     6481 gcgtgccggt gtagaacatc agaccggcag gcgccgtgga gttgggtagc caggttgttt
     6541 tgggctcttc ataggtgggg ccgtggatac cgccattgac ccagcaaccg gacgtgttgt
     6601 tggcaaagct gtaggg
//
LOCUS       DAOSJM010000097         6614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10545_length_6614_cov_5.147126, whole genome shotgun sequence.
ACCESSION   DAOSJM010000097 DAOSJM010000000
VERSION     DAOSJM010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10545_length_6614_cov_5.147126"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PKX82_04875"
     CDS             <1..193
                     /locus_tag="PKX82_04875"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOF51616.1"
                     /translation="TVEYKVNFLAAFRDGELRAIGRVVKAGRRVIVTTAELVHVDDQG
                     VRNPCAVMQQTLMPVPKTY"
     gene            225..1406
                     /locus_tag="PKX82_04880"
     CDS             225..1406
                     /locus_tag="PKX82_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HOF51617.1"
                     /translation="MNNLPGLNFQLGEDIDALRDAVREFAQAEIAPRAAEIDRNDQFP
                     MDLWQKMGSLGVLGITVGEEYGGANMGYLAHMIAMEEISRASASVGLSYGAHSNLCVN
                     QIKRNGTDAQRAKYLPKLISGEHVGALAMSEPGAGSDVLSMKLKAEDKGGYYLLNGNK
                     MWITNGPDADTLVVYAKSEPELGARGVTAFLIEQGMKGFSIAQKLDKLGMRGSHTGEL
                     VFNNVEVPHANVLGQVNGGAKVLMSGLDYERAVLTGGPLGIMQSVMDNVIPYIHDRKQ
                     FGQSIGEFQLIQGKVADMYTVLQAGRAFAYTVAKNLDLLGVEHVRQVRKDCASVILWT
                     AEKATWMAGEGVQIFGGNGYINEYPLGRLWRDAKLYEIGAGTSEIRRMLIGRELFAET
                     M"
     gene            1410..3422
                     /locus_tag="PKX82_04885"
     CDS             1410..3422
                     /locus_tag="PKX82_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOF51618.1"
                     /translation="MKSAITRTSRSLLQGVLRGLMRAWVLVVPLVVALSLIRLGQLAY
                     FWPTGYSASTTDLTTIVVQGFRFDLKVSAIAGFLLLLVLPWVSGKAHGRIAAVVAFVY
                     VMLSLVNLHYFGFYKTPIDSLVFGLVEDDTAAVLQTIWRDFPVVWTLLLAAVLTWASV
                     WLHGRLVRRMQPDTLLQTHHAAVKVVLVVVVFFALLFAGKGTIREMALQRQHLTVTTS
                     QFLNDMVPNGVIALKYAWDSRGQSQNLSDPMVGLKAMGFDSPLAAAQALGLPHGNEAE
                     VKAALTAHDPIPAGMRKKNLVFFLMESWSAEPLLYQGPKFDVLGRLAPTLTDPGRACH
                     FRNFDSAHAGTHPSLEAILFSTPITPLTLGDVGRKPIPWAIPKVVRDAGYQTLFVTSA
                     RSGWRDLNRVLKVQGFDEVVDANTLKEAYPEAVLGIWGVWDSYVFKYLSKRMAAQPAD
                     KPLFVFVLTSTNHPPYDLPADYKRVPRDMSLWKGETSSDTLVPNLDSYHYAADLLGGF
                     VQEVQKGPLHAHTLVAATGDHNVRSFGVYAEANRRYLMRQVPFVIWGDNLQCGNQQAL
                     PASHRDMFNTLLPLAGIAGPYVNSGRNLLRPTTPSPDPVNAPRAMFFTGEARNAKGLW
                     QLGNKNSFVCSAATPEADCAFNALDDQQERARYALLDWNVRVSLRK"
     gene            3431..4096
                     /gene="can"
                     /locus_tag="PKX82_04890"
     CDS             3431..4096
                     /gene="can"
                     /locus_tag="PKX82_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HOF51619.1"
                     /translation="MSSELDELFAHNRAWAAQMEKERPGFFTSLKSQQKPRYMWIGCS
                     DSRVPANQITGLEPGEVFVHRNVANVVVPTDLNCLSTIQFAVDQLKVEHLMVVGHYGC
                     GGVRAALEDIRVGLADNWLRHVKDVRDRHRHLLSMLPEHGRVDALCELNVIEQVLNVA
                     YTTVMQDAWSRGQNVTLHGWIYGLQDGLLQNLHMTVSGNDELEALYAKALSGVRLMPR
                     HPA"
     gene            4118..5944
                     /gene="aceK"
                     /locus_tag="PKX82_04895"
     CDS             4118..5944
                     /gene="aceK"
                     /locus_tag="PKX82_04895"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HOF51620.1"
                     /translation="MFPTRLDSSVAYDIAKAMMDGFNRHYRLFRTESARAKHRFETAD
                     WHGQQRAQRERIEFYDLRVLECSNRLEREFKAGEQPMDIWQQIKLHYIGLLVNHHQPE
                     LAETFFNSVTTKILHRSYFQNDFIFVRPAVSTEYIENDETNKRPSYRSYYPTRETVQQ
                     VLRQMVEDFDLRLRFEDLDRDTTWVTQAMQGWLGDGKLRANFQIQVLTGLFFRNKGCY
                     IVGKAINGFYEFPFALPVLHGASGKLVIDAVLSGEDDLLMLFSFARAYFMVDMEIPSA
                     YVQFLRSMMPRKPRNEIYNALGLAKQGKTLFYRDFLHHLRHSTDKFRIAPGIKGMVML
                     VFDLPSFPYVFKVIKDFYPPPKDTTREQIKGKYLLVKQHDRVGRMADTLEYSEVGFPR
                     DRFDDALIAEIEKFAPSQLEISDRDGDGNVEVIIKHVYIERRMIPLNIYLQEAFDAGG
                     ANPDDPSPAAARARDQIERGVIEYGNAIKDLVAANIFPGDMLWKNFGIMRQSKVVFYD
                     YDEIEYVTDCNFRKVPEPRNEEEEMSGEVWYTVGPRDVFPETFAPFLLGNPKVREVFM
                     QHHADLLDPVFWQSHKDRIQAGYVYDVFPYDQDKRFRQTPVA"
     regulatory      5972..6072
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6159..>6614
                     /locus_tag="PKX82_04900"
     CDS             6159..>6614
                     /locus_tag="PKX82_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914256.1"
                     /note="catalyzes the formation of
                     4-amino-2-methyl-5-phosphomethylpyrimidine from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole and
                     S-adenosyl-L-methionine in thiamine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOF51621.1"
                     /translation="MNAPDKLAQFIRLTREPLPASTKRYLQGSRPDIQVPLREILQSN
                     GEAITVYDTSGPYTDPRADIDVRQGLPLVRQGWVEGRGDTELYVGRPPFALDDGLKNG
                     HTSEGGDVLAALRAQAAGLQRQPRRSKAGGNVTQMHYARKGIITPEMEYV"
BASE COUNT         1281 a         2011 c         2022 g         1300 t
ORIGIN      
        1 gaccgtggaa tacaaggtca acttcctcgc cgcgttcagg gacggcgagc tgcgtgccat
       61 tggccgggtg gtcaaggcgg gccgccgcgt catcgtcacc acggcggagc tggtgcatgt
      121 ggacgaccag ggcgtacgta acccctgtgc cgtcatgcag cagaccctga tgcctgttcc
      181 caaaacttac tgattcatca cgttcaaaac tcaaggagac aaccatgaac aatttgcccg
      241 gcttgaactt ccagctcgga gaagacattg acgcattgcg tgacgcggtg cgcgaattcg
      301 cccaggccga gatcgcaccc cgcgctgccg aaatcgaccg caacgaccag tttccgatgg
      361 acctgtggca gaagatgggc agcctgggtg tgctgggcat caccgtcggc gaagagtacg
      421 gcggcgccaa catgggctac ctggcgcaca tgatcgccat ggaagaaatt tcgcgtgctt
      481 ctgcgtcggt gggcctgtcc tacggcgcgc acagcaatct gtgtgtgaac cagatcaagc
      541 gcaacgggac cgacgcccag cgcgccaaat acctgcccaa gctgatctcc ggcgagcatg
      601 tcggtgcgct ggccatgagc gagccaggcg ccggctccga cgtgctgagc atgaagctca
      661 aggccgagga caagggcggc tactacctgc tcaacggcaa caagatgtgg atcaccaacg
      721 gccccgacgc cgacacgctg gtcgtctacg ccaagagcga acccgaactg ggcgcacgtg
      781 gcgtcaccgc tttcctgatc gagcagggaa tgaaaggttt ctccattgcg cagaagcttg
      841 acaagctggg catgcgcggc agccacacgg gtgagctggt gttcaacaac gtcgaagtgc
      901 cccacgccaa tgtgctgggc caggtcaacg gcggcgccaa ggtgctgatg agcggtctgg
      961 actacgagcg tgcggtgctg accggtggcc ccctgggcat catgcagtcc gtcatggaca
     1021 acgtcatccc ctacatccac gaccgcaagc agttcggcca gagcatcggc gaattccagc
     1081 tgatccaggg caaggtggcc gacatgtaca ccgtgctgca ggccggccgc gcctttgcct
     1141 acaccgtggc caagaacctg gatctactgg gtgtggagca tgtacgccag gtgcgcaagg
     1201 actgcgcatc cgtcatcctg tggacggccg agaaggccac ctggatggcc ggcgagggcg
     1261 tgcagatctt tggcggcaat ggctacatca acgaataccc gctgggccgc ctctggcgcg
     1321 atgccaaact ctatgaaatc ggggcgggca ccagtgagat tcgccgtatg ctgattggtc
     1381 gggagctgtt cgctgagaca atgtaaggca tgaaatctgc cattacccgt acctcccgct
     1441 ccctgcttca aggcgtcttg cgcggactga tgcgcgcatg ggtgctggtg gtaccgctgg
     1501 tggtggcgct ttcactgatc cggctggggc agctggccta tttttggccg accggctaca
     1561 gcgcgtctac cactgacctc accaccatcg tggtgcaggg cttccgcttt gacctcaagg
     1621 tcagcgctat tgcgggtttt ttgctactgc tggtgttgcc ctgggtctcg ggcaaagcgc
     1681 atgggcgcat cgctgcggtg gtggcgtttg tctatgtgat gctgtcgctg gtcaatctgc
     1741 actacttcgg cttttacaag acgccgattg attcactggt gttcggtctg gtggaggacg
     1801 acaccgctgc tgtactgcag accatctggc gtgactttcc cgttgtctgg acgctgctgc
     1861 tggcggctgt gctgacctgg gcctcggtct ggctgcatgg cagactggtc cggcgcatgc
     1921 agcccgatac gctgctgcag acccaccatg ccgccgtcaa ggtggtgctg gtggtggtgg
     1981 tgttctttgc cctgctgttt gcgggcaaag gcaccatccg ggagatggcc ctgcagcgcc
     2041 agcacctgac cgtcaccacc tcgcagttcc tcaacgacat ggtgcccaat ggcgtcattg
     2101 ctctgaagta cgcgtgggac agccgcggcc agtcgcagaa cctctccgat ccaatggtgg
     2161 gcctcaaggc catgggtttt gattcaccgc tggcggcggc ccaggcgttg ggtctgcccc
     2221 atggcaatga ggcagaggtc aaggccgcgt tgacggccca cgacccgatt cccgcaggta
     2281 tgcgcaaaaa gaacctggtg ttcttcctga tggagtcctg gagcgcagag ccgctgctgt
     2341 accaaggccc gaagttcgat gtgctcggac gcctggcacc caccctgacg gatcctggcc
     2401 gggcctgcca cttccgcaac tttgactcgg cccatgcagg tactcacccg tcgctggaag
     2461 ccatcctttt ttccaccccc atcacgccgc tgacgctggg tgatgtgggg cgcaagccca
     2521 ttccctgggc gattccgaag gtggtacgcg atgcgggtta ccagaccctg tttgtgactt
     2581 cggcacggtc gggctggcgt gacctcaacc gcgtgctcaa ggtgcagggc ttcgatgaag
     2641 tggtcgatgc caacaccctc aaggaggcct acccggaggc ggtgctgggc atctggggcg
     2701 tgtgggacag ctatgttttc aaatacctca gcaagcgcat ggctgcgcag cccgccgaca
     2761 agccgctgtt tgtgtttgtg ctgacctcta ccaaccatcc gccgtacgac ctgcctgcgg
     2821 actacaagcg tgtgccgcgc gacatgtccc tgtggaaagg cgagaccagt tccgacaccc
     2881 tggtgcccaa cctggacagc taccactatg cagcggacct gctgggcggt tttgtgcagg
     2941 aagtgcaaaa gggccctctg catgcccata ccctggtggc ggccaccggg gaccacaatg
     3001 tgcggtcctt tggtgtctac gccgaagcca accggcgcta cctgatgcgc caggtgccgt
     3061 ttgtcatctg gggcgacaac ctgcagtgcg gcaaccagca ggcgctgccc gccagccacc
     3121 gtgatatgtt caacaccctc ttgccactgg ctggcattgc cgggccgtac gtgaattcgg
     3181 gccgcaacct gttgcgtccc accacgccat cgccggaccc tgtgaatgcg ccgcgcgcca
     3241 tgtttttcac cggtgaggcc cgcaacgcca aaggcctgtg gcaactgggg aataaaaact
     3301 cgtttgtctg cagtgctgcc acacctgagg cagactgcgc attcaacgca ctggatgacc
     3361 agcaggagcg cgcacgttac gcgctgctgg actggaatgt gcgtgtgtcc ctcaggaaat
     3421 aaggtctttc atgtccagcg aactcgacga actttttgcc cacaaccgtg cctgggctgc
     3481 acagatggag aaagaacgcc ccggtttttt caccagcctg aagagtcagc agaagccgcg
     3541 ctacatgtgg attggctgtt ccgacagccg ggtacccgcc aaccagatca cggggctgga
     3601 gcctggcgaa gtgtttgtcc accgcaatgt ggccaatgtc gtggtcccca ccgacttgaa
     3661 ttgcctgtcc acgatccagt tcgctgtgga tcagctcaag gtggagcacc tcatggtggt
     3721 ggggcactac ggatgcggcg gtgtgcgtgc tgctctggaa gatatccgtg tggggctggc
     3781 cgacaactgg ttgcgccacg tcaaggatgt gcgcgaccgc caccgtcacc tgctttccat
     3841 gctgccggaa catggccggg tcgatgcgtt gtgcgagctg aacgtgattg agcaggtgct
     3901 caatgtggcc tacaccaccg tgatgcagga cgcctggagc cgcgggcaaa atgtcacgct
     3961 gcatggctgg atttatggtt tgcaggacgg cctgctgcaa aacctgcaca tgacggtcag
     4021 cgggaatgac gagctggaag ccctctatgc caaggctctg tcgggcgtgc gcctgatgcc
     4081 gcgccatccg gcataagcag atggcggttg gcgagacatg tttcccaccc gtctggattc
     4141 atcggtagcc tacgacatcg ccaaggcgat gatggatggg ttcaaccggc actaccggct
     4201 gttccgcacc gaatcggccc gcgccaagca ccggttcgag acggccgatt ggcatggcca
     4261 gcaacgcgcg cagcgcgagc gcatcgagtt ctacgacttg cgtgtgctgg agtgctccaa
     4321 ccgcctggaa cgcgagttca aggcgggcga gcaacccatg gacatctggc agcagatcaa
     4381 gctgcactac atcggcctgc tggtcaacca ccatcagccg gaactggctg agacgttttt
     4441 caactcggtc accaccaaga tcctgcaccg cagctacttc cagaacgact tcatcttcgt
     4501 gcgcccggcg gtcagcaccg aatacatcga gaacgacgaa accaacaagc gcccatcgta
     4561 ccgctcgtat taccccaccc gcgagacggt gcaacaggtt cttcgccaga tggtggagga
     4621 ctttgacttg cgcctgcgct ttgaagacct ggaccgcgac accacctggg tgacgcaggc
     4681 catgcagggc tggctgggcg acggcaagct gcgcgccaac ttccagattc aggtgctcac
     4741 tggcctgttc ttccgcaaca agggctgcta catcgtgggt aaggccatca atggctttta
     4801 tgagtttccg tttgcactgc cggtgctgca tggcgccagt ggcaagctgg tgatcgatgc
     4861 cgtgctgtcg ggtgaggacg acctgctgat gctgttcagc tttgcgcggg cttacttcat
     4921 ggtggacatg gaaatcccat cggcctacgt gcagttcctg cgcagcatga tgccgcgcaa
     4981 gccgcgcaac gaaatctaca acgcactggg gctggccaag cagggcaaaa ccctgttcta
     5041 tcgcgatttc ctgcaccacc tgcgccacag taccgacaag ttccgtatcg ctcccggtat
     5101 caagggcatg gtcatgctgg tatttgatct gcccagcttt ccatatgtgt tcaaggttat
     5161 caaggatttt tatccaccac ccaaggacac cacgcgcgag cagatcaagg gcaagtacct
     5221 gctggtcaag cagcacgacc gcgtgggccg catggccgac acgctggagt acagcgaggt
     5281 cggttttccg cgggaccggt ttgacgatgc gctgattgcc gagatcgaga agttcgctcc
     5341 cagccagctg gagatcagcg accgggatgg cgacggcaac gtcgaagtca tcatcaagca
     5401 cgtgtacatc gagcggcgca tgatcccgct caacatttac ctgcaggaag cctttgatgc
     5461 cggtggtgcc aacccggacg atccaagccc tgccgcggcg cgcgcacgcg accagatcga
     5521 gcgtggtgtc atcgagtacg gcaatgccat caaggacctg gtggcggcca atatctttcc
     5581 gggcgacatg ctgtggaaga acttcggcat catgcgccag agcaaagtgg tgttttacga
     5641 ttacgacgag attgagtacg tcaccgactg caatttccga aaggtgcccg aaccgcgcaa
     5701 cgaggaagag gaaatgtcgg gcgaggtctg gtacaccgtg ggcccgcgcg atgtatttcc
     5761 cgaaaccttt gcgccctttc ttctggggaa tcccaaggtg cgcgaggtgt tcatgcagca
     5821 ccatgcggac ctgctcgatc ctgtcttctg gcagagccac aaggaccgca tccaggcggg
     5881 ttatgtctac gatgtctttc cctacgatca ggacaagcgc ttccgccaga ccccggtggc
     5941 ctgacctcgg ttaaggtgtt tgtcgtacac tgcgccttgc ccggggtgcg cagtggttgc
     6001 gctgagatgg attccaaacc cgtgaacttg atctggttca taccagcgta agaagtgcag
     6061 agcagtgccc caggacgtgt gccgtgcggg cggtctcatt ccaggttcac ggggttgtgt
     6121 ctttctctgt cttcgggcgt taacccggag attttccagt gaacgcaccc gacaaactcg
     6181 cccagttcat tcgcctcacg cgcgaaccgc ttcccgcctc caccaagcgt tatctgcaag
     6241 gcagtcgccc tgacatccag gtgccgctgc gcgaaatcct gcagagcaat ggcgaagcca
     6301 tcacggtgta cgacacctca ggtccttaca ccgacccgcg cgcggatatt gacgtgcgcc
     6361 agggcctgcc gctggtgcgc cagggttggg tagagggccg gggagatacc gagctgtacg
     6421 tgggccgtcc gccgtttgcg ctggacgatg gtctgaaaaa cggccataca tcggaaggtg
     6481 gcgatgtact ggccgccctg cgcgcccaag ccgctggcct gcagcgccag ccacgccggt
     6541 ccaaagcggg cggcaacgtc acgcagatgc actacgcgcg caaaggcatc atcacccccg
     6601 aaatggaata cgtg
//
LOCUS       DAOSJM010000098         6605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10561_length_6605_cov_5.167176, whole genome shotgun sequence.
ACCESSION   DAOSJM010000098 DAOSJM010000000
VERSION     DAOSJM010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10561_length_6605_cov_5.167176"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1154
                     /locus_tag="PKX82_04905"
     CDS             <1..1154
                     /locus_tag="PKX82_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51622.1"
                     /translation="KINRFADKDSHQIFLEPEGLTTHEVYPNGISTSLPFDIQYALVR
                     SMPGLENAHILRPGYAIEYDYFDPRSLKSSFETRQIGGLFFAGQINGTTGYEEAAAQG
                     MFAGINAALQCREQSAWMPGRDEAYLGVLVDDLITKGVTEPYRMFTSRAEFRLQLRED
                     NADARLTEAGRKLGLVDDARWDAFCRKRDAVSRETERLRSIWVNPKNLPASESERVLG
                     KSIDHEYNLADLLRRPDVSYAGLMSLSDGKYANPELPVKLTAGVSRETSEAASVIAQD
                     VFVAAVVEQVEIAAKYSGYIDRQKDEVERAAHYENLRLPAELDYMQVSALSIEARQRL
                     NKFRPETLGQASRLSGITPATISLLLIHLKKGNFKGFVEKAGAAGEVAA"
     gene            1154..1816
                     /gene="rsmG"
                     /locus_tag="PKX82_04910"
     CDS             1154..1816
                     /gene="rsmG"
                     /locus_tag="PKX82_04910"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOF51623.1"
                     /translation="MHPLDKGLRDGLQKLALSLDETQIARLLGYLDLIQKWNKVYNLT
                     AVRDPAEMLTHHLLDSLAVIAPLRRQLESMGLGGKPVRLLDVGSGAGLPGVVIAVCCP
                     DIAVHCVDTVAKKAAFIQQVAVSLKLPNLRGIHARVESLTEPYDVVSSRAFASLVDFV
                     TWSEKALAGQGVWMGMKGKHPADEMAALPSSVKVFHVEQLDVPGLGAERCIVWMRKAV
                     VA"
     gene            2168..2782
                     /locus_tag="PKX82_04915"
     CDS             2168..2782
                     /locus_tag="PKX82_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOF51624.1"
                     /translation="MLGVTDYGTFVVTIIVFLLIPGPGNLALITSTSKGGILGGLAAT
                     LGVILGDQVLMWSAVAGVAALLAAYPQAFEAVQWVGAGYLAWLGFKMLLAKPGAAPVL
                     NIKAGHYLRQAMAITLLNPKAILFYMAFFPLFVDPARQQGLLTYGFMAATIAVLTFFY
                     GLTSTLLTHFLAERVRANPVISRVLEKVAGLCLVGFGIKLAISR"
     gene            2854..3639
                     /locus_tag="PKX82_04920"
     CDS             2854..3639
                     /locus_tag="PKX82_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOF51625.1"
                     /translation="MAKIFCVANQKGGVGKTTTTVNLAAGLAKVGQRVLMIDLDPQGN
                     ATMGSGVDKRKLELTVYDVLLESASITEARAQSDKLKEGGCSYDILGANRELAGAEVE
                     LVEVERRERRLKQAIAAVDSEYDFVLIDCPPSLSMLTLNGLCCAHGVIVPMQCEYFAL
                     EGLTDLVNTIKQVKANLNDDLQIIGLLRVMFDPRITLQQQVSDQLKAHFGDKVFDSVI
                     PRNVRLAEAPSYGVPGVVFDPNSKGAQAFVAFAKEMVARIEAM"
     gene            3640..4191
                     /locus_tag="PKX82_04925"
     CDS             3640..4191
                     /locus_tag="PKX82_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOF51626.1"
                     /translation="MESRNVLVLPGWQNSGPEHWQSRWEALHGYVRVQQHDWMRPLRG
                     DWMAQLEEAILAATGPVVLVAHSLGCMLTAAWAAHSQNTHRVVGALLVAPGDPEREEL
                     RAVLKSWSPVVMQRLPFKSVLLGSQNDPYCSLERARAFAHAWGSEFVDYGRAGHINAE
                     SGLGDWPDGHALLKNLMLTKEEK"
     gene            4191..5123
                     /locus_tag="PKX82_04930"
     CDS             4191..5123
                     /locus_tag="PKX82_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOF51627.1"
                     /translation="MVTKKPKGLGMGLEALLGPKVADRAADGAIPTNPGQPNTLLLTD
                     MVAGMYQPRTRMDEGALNELAESIKVQGIMQPILVRKLSDGANAGKFEIIAGECRFRA
                     AKLAGLDSVPVLVRDVPNEAAAAMALIENIQREDLNPLEEAHGLQRLIKEFGLTHETA
                     AQAVGRSRSAASNLLRLLNLADPVQTMLMAGDIDMGHARALLALDRATQITAANQVAT
                     KKLSVREAESLVKKLSAEFALTPQKPKKEKSRDLKRVEEELSDLLMAQVDVHIKKRVK
                     RNGRFEEMGELVIQFGSLDELNGLIERLSPGTSR"
     gene            5258..5971
                     /locus_tag="PKX82_04935"
     CDS             5258..5971
                     /locus_tag="PKX82_04935"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF51628.1"
                     /translation="MAAWALFKLALGQGVALGLAMALATALGVAASLWGNSWWRRVLI
                     AAGFPLSLALSLASLGVSGVPAWAWLLPLLLLLLVYPINAWRDAPLFPTPPDALDALP
                     EYAPLHDGARVLDAGCGLGQGLEALNRAYPHVHLHGLEWSWPLRWMCALRCPWAEVRQ
                     GDIWKADWSCYDLVYLFQRPESMPRAWEKAAAELRPGAWLVSLEFEVAGHISSTQYQA
                     PDGRMVWLYRAPLSAHSGE"
     gene            5974..>6605
                     /locus_tag="PKX82_04940"
     CDS             5974..>6605
                     /locus_tag="PKX82_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOF51629.1"
                     /translation="MDSAPVPLGDDSAQAAQALRVAFFAMATLAELRESDTESHLLRV
                     QRYVRVLADKLKSHPAFAPTLTQGYIDLLCASVPMYDMGTVGIPDRILLKPGRLAPEE
                     LAIMRTHTTLGFDALVRAEKTLGQTSPLLAIAKEITLSHQEKWDGNGYPQGLAGTKIP
                     LSARILAVADVYDALISNKVYKQGVEHEAAVQVIFSERASHFDPDVVDAF"
BASE COUNT         1217 a         2006 c         2127 g         1255 t
ORIGIN      
        1 acaagatcaa ccgctttgcc gacaaggaca gccaccagat ttttctggag cccgagggcc
       61 tgaccacgca cgaggtctac cccaacggga tttcaacgtc gctgcccttt gatatccagt
      121 acgcactggt gcgcagcatg cctgggttgg agaacgcgca catcctgcgc cccggctacg
      181 ccatcgagta cgactacttt gacccccgca gtttgaagag cagtttcgag acgcgccaga
      241 ttggtggcct gttctttgcc ggccagatca atggcaccac cggctatgaa gaggcggcgg
      301 cgcagggcat gtttgccggc atcaatgccg cattgcagtg ccgggagcag agtgcctgga
      361 tgcccggacg cgatgaggcc tacctcggcg tgctggtaga cgacctgatc accaagggcg
      421 tgaccgagcc ctaccgcatg tttaccagcc gtgcggagtt ccggctgcag ttgcgcgaag
      481 acaatgccga cgcccggctg accgaggctg gccgcaagct ggggctggtg gacgacgccc
      541 gctgggacgc cttctgccgc aagcgcgatg ctgtttcacg tgaaacagag cgcctgcgca
      601 gcatctgggt gaatcccaaa aacctgccgg catcggagtc cgagcgggtg ctgggcaagt
      661 ccattgacca tgaatacaac ctggccgacc tgctgcgtcg gcccgatgtg agctatgccg
      721 gtctgatgtc gctaagcgac ggcaaatacg ctaacccgga gctgcccgtg aaattgacag
      781 ctggggtttc acgtgaaaca agcgaagcag ctagcgtcat tgcgcaagac gtctttgtgg
      841 ccgcggtggt cgagcaggtg gaaattgctg ccaagtattc gggctacatc gaccgccaga
      901 aggacgaagt cgagcgtgcc gcccattacg agaatctgcg cttgccggcc gagctggact
      961 acatgcaggt gtcggccctg agcatcgagg cacgccagcg gctcaacaag ttccgccccg
     1021 aaacgctggg gcaggcgtcg cgcctctccg gcatcacgcc tgccaccatt tccctgttgc
     1081 tgatccatct gaagaaaggc aacttcaagg gttttgtcga gaaggccggc gcggcgggtg
     1141 aggtggctgc ctgatgcatc cgctggataa aggcttgcgc gacgggctgc agaagctggc
     1201 tctgtcgctg gatgaaaccc aaatagcccg gttgctggga tacctcgacc tgatccagaa
     1261 atggaacaag gtctacaacc tgacggcagt gcgggaccca gccgagatgc tgacccacca
     1321 cctgctcgac agtctggcgg tgattgcgcc gctgcgccgc caactggaat ccatggggct
     1381 gggcggaaag ccggtgcggc tgctggatgt cggttcgggt gccggactgc cgggcgtggt
     1441 gattgccgtc tgctgtccgg acatcgccgt gcattgtgtg gacacggttg ccaagaaggc
     1501 agcgttcatt cagcaggtgg cggtgagttt gaagttgccc aatctacgag gcatccatgc
     1561 gcgggtggaa agtttgaccg aaccctatga cgtggtcagt tcccgggctt tcgcatcgct
     1621 ggtggatttt gtgacctggt ccgaaaaggc cttggcaggg cagggcgtct ggatggggat
     1681 gaagggcaag caccctgcgg acgagatggc tgcgttgccg agttcggtga aggtgtttca
     1741 cgtggaacag ttggatgtgc cggggcttgg ggcagagcgg tgcattgtgt ggatgcggaa
     1801 agctgtggtc gcttgagttt tcttggcgtg cgtgcgccgc ttcgggatcg actgcggtat
     1861 gcgttttgct ccgtgtcccc cgcccttcgg gctcctcctt tacctgcgca aaacgcatac
     1921 cccagccaat cttgcgcggc ttgggtcgtt gcggcccgca ccctaaaccc agtccgcgct
     1981 cagaggcgtg gggtgagtgc caaagtgagg taaaggggga ggccgaaggc cggaggacac
     2041 gaacggcggc acttaccccg cgactccagc ggacgtgcga gaaccaacca acccatgcaa
     2101 catgtcgaag ttcggcaacc cgcatttcca acgtaaactc acatccttcc ccaatacaaa
     2161 gaatcccatg ctcggcgtca ccgactacgg cacttttgtc gtcaccatca tcgtgttcct
     2221 gctgattccc ggtcccggca atctggcact gatcacgtcc accagcaagg gcggcatcct
     2281 gggtggactg gcggcaaccc tgggtgtgat cctgggtgac caggtgctga tgtggtctgc
     2341 ggtcgccggt gtggcagctt tgctggctgc ctaccctcag gccttcgagg cggtgcaatg
     2401 ggtgggcgct ggctatctcg cctggctggg attcaagatg ctgctggcca aacccggtgc
     2461 cgcaccggta ctcaacatca aggccgggca ttacctgcgc caggccatgg cgatcacgct
     2521 gctcaacccc aaggccatcc tgttttacat ggcgttcttt cccttgtttg tcgatccggc
     2581 gcgccagcag ggcctgctga cctatgggtt catggcagct accattgcgg tgctgacctt
     2641 tttctatggt ctgacttcca cgctgctgac gcactttctg gcggagcgcg tacgtgccaa
     2701 ccccgtgatc tcgcgggtgc tggagaaagt ggccgggctg tgcttggtcg gatttggtat
     2761 caagctcgcc atctcccgct aggcccgcac ggcaacgttc cacgtgaaac atcccatctc
     2821 cgcttcttct tcttttctct catccggtat cccatggcca aaatcttctg tgtagccaat
     2881 caaaagggtg gtgttggcaa aaccaccacc acagtcaacc ttgcggcagg ccttgccaag
     2941 gtcgggcagc gggtgctgat gattgacctc gacccgcagg gcaacgccac catgggttcc
     3001 ggggtggaca agcgcaagct cgagttgacg gtgtacgacg tactgctgga gtcggcctcg
     3061 atcaccgagg cgcgcgcaca aagcgacaag ttgaaagagg gcggttgcag ctacgacatc
     3121 ctgggtgcca accgcgaact tgccggcgcc gaagtggagc tggtggaagt ggagcgtcgc
     3181 gaacgccgcc tcaagcaggc gattgcggcg gtggattccg aatacgactt tgtgttgatc
     3241 gactgcccgc catcgctctc gatgctgacg ctcaacgggc tgtgctgcgc ccatggcgtg
     3301 atcgtgccca tgcaatgcga gtactttgcg ctcgaaggcc tgaccgatct ggtcaacacc
     3361 atcaagcagg tcaaggccaa tctcaacgac gacctgcaga tcatcggatt gctgcgcgtc
     3421 atgttcgatc cgcgtatcac cctgcagcag caggtcagcg accagctcaa ggcccacttc
     3481 ggcgacaagg tgtttgactc cgtcattccc cgcaacgtgc gcctggccga agcgcccagc
     3541 tacggcgtgc cgggtgtggt gtttgatccc aactccaagg gtgcgcaggc atttgtcgcc
     3601 ttcgccaaag agatggtggc gcgcatcgag gcgatgtagg tggagtcccg taacgtcctg
     3661 gtattgccgg gttggcaaaa ctccggccct gaacactggc aaagccggtg ggaagcgttg
     3721 cacggctatg tgcgcgtgca gcagcatgac tggatgcgcc cactgcgtgg cgactggatg
     3781 gcgcaactgg aggaggccat tctggccgcc accgggccgg tggtgctggt ggcgcacagc
     3841 ctgggttgca tgctgactgc ggcctgggcg gcgcattcgc agaacacgca tcgcgtggtc
     3901 ggggccttgc tggtggcgcc cggcgacccc gaacgcgagg agttgcgcgc ggtgctcaag
     3961 agctggtcgc cggtggtgat gcaacgtctg ccgtttaaga gtgttttgct gggcagccag
     4021 aacgatccct attgcagcct ggagcgcgca cgcgcctttg cccatgcctg gggttcggag
     4081 ttcgtggatt acggacgggc cgggcatatc aatgccgaat cgggactggg tgactggcct
     4141 gacggccacg cactactgaa gaatttgatg ctaacgaagg aagaaaaata atggtcacca
     4201 aaaaacccaa agggctgggc atgggtctgg aagcattgct cggccccaag gtggcggacc
     4261 gcgccgctga cggtgccatc cccaccaacc ccggccagcc caacaccttg ctgctgaccg
     4321 acatggtggc cggtatgtac cagccgcgca cccgcatgga cgagggcgcc ttgaacgagc
     4381 tggccgagtc gatcaaggtg cagggcatca tgcagccgat cctggtgcgc aagctgtcgg
     4441 acggggcgaa tgccggcaag tttgaaatca tcgcgggcga gtgccgcttc cgcgcggcca
     4501 agctggccgg gctggacagc gtgccggtgc tggtacgcga cgtgcccaac gaggccgccg
     4561 ccgccatggc gctgatcgag aacatccagc gcgaagacct caacccactg gaagaggccc
     4621 acggcctgca gcgcttaatc aaagagtttg ggctcactca cgaaactgcc gcgcaggcgg
     4681 tgggccgttc gcgcagtgcc gccagcaacc tgctgcgcct gctgaatctg gccgacccgg
     4741 tccagaccat gctgatggcc ggcgacatcg acatgggcca cgcccgtgcc ctgttggccc
     4801 tggaccgcgc cacccagatc accgcggcca accaggtcgc caccaaaaaa ctgtcggtgc
     4861 gcgaagccga aagcctggtg aaaaagctca gcgccgaatt tgcgttgacc ccgcagaaac
     4921 ccaagaaaga gaaatcccgc gacctcaagc gtgtggaaga agaactctcc gacctgctga
     4981 tggcgcaggt cgatgtgcat atcaagaagc gtgtcaagcg caatggccgg ttcgaagaga
     5041 tgggcgagct ggtcatccag tttggctcgc tcgacgaact caatggcctg atcgaacgcc
     5101 tgtcgcccgg caccagccgc tgaatctgct gagttgctat ttatttcata gctgcttgcg
     5161 catactccat gcgggccaga ggccaatacc acactcaatc ggtggcaccg tgtccgagcg
     5221 cctgatcgtc tggcctgtgc cagccgtatt ggcctggatg gcggcctggg cgctcttcaa
     5281 gctggccctg gggcaaggcg tggcgctggg cctggccatg gcgctggcca ccgcgctggg
     5341 cgtggccgcc agtctgtggg gaaactcctg gtggcgccgg gtgctgattg ccgccgggtt
     5401 tccgctgtcg ctggccctga gcctggcgtc gctgggtgtg tccggcgtgc cggcgtgggc
     5461 ctggctgctg ccgttgctgc tgctgctgct ggtctatccg atcaatgcct ggagggatgc
     5521 cccgctgttt cccacgccac cggatgcgct ggacgcactg cccgagtacg cgcctctgca
     5581 cgatggtgcg cgcgtgctcg atgcgggctg cggtctgggg caaggtttgg aggccctgaa
     5641 ccgcgcctac ccgcatgtgc acctgcacgg actggaatgg agctggccgc tgcgctggat
     5701 gtgcgcgctg cgctgcccct gggccgaagt gcgccagggc gacatctgga aagccgactg
     5761 gtcctgctac gacctggtgt acctgttcca gcgccccgag agcatgccgc gcgcctggga
     5821 gaaggctgca gccgagttgc gaccgggggc ctggctggtc agtctggaat ttgaagtggc
     5881 cggtcacatt tccagcaccc agtaccaggc gcctgatggc agaatggtgt ggctgtaccg
     5941 ggcgccgctc tcggcgcact caggagaata gacatggatt ctgcccccgt ccctttgggt
     6001 gacgattctg cgcaagccgc ccaggcgctg cgcgttgcct ttttcgccat ggccacgctg
     6061 gcagagctgc gcgagtccga caccgagagc cacctgctgc gggtgcaacg ctatgtgcgt
     6121 gtactggccg acaaactcaa aagccaccca gcttttgccc ccacgttaac gcaggggtac
     6181 attgatctgc tgtgcgccag cgtaccaatg tacgacatgg gcacggtggg cattccggac
     6241 cgcatcctgc tcaagccggg gcggctggcc ccggaagagc tggccatcat gcgcacccac
     6301 accacgctgg gtttcgacgc gctggtgcgt gccgaaaaaa ccctgggcca gacgtcgccc
     6361 ctgctggcca ttgccaagga aatcaccctg tcccaccagg aaaaatggga tggcaacggc
     6421 tacccgcagg gtctggcggg cacgaagatt ccgctgtctg cccgcattct ggccgtggcc
     6481 gatgtgtacg acgcgctgat cagcaacaag gtctacaagc agggcgtgga gcacgaggcc
     6541 gcggtgcagg tgatcttcag cgagcgcgcc tcccacttcg accccgatgt ggtggatgcc
     6601 ttcat
//
LOCUS       DAOSJM010000099         6579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10631_length_6579_cov_4.170141, whole genome shotgun sequence.
ACCESSION   DAOSJM010000099 DAOSJM010000000
VERSION     DAOSJM010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10631_length_6579_cov_4.170141"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="PKX82_04945"
     CDS             complement(<1..1126)
                     /locus_tag="PKX82_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HOF51630.1"
                     /translation="MKTPSPFPAPASRQSAICNSGFRWHHLALPCCLALALIGATLPK
                     SHAATAEHGATADHGTPKPVAATKASAAKPVQASSAEPAEGKSLAGDLRSALKDQLID
                     KKSLTLVIKDNPPAKPAAASIKEPEHVAPPSSRAMAPVAPAVAAHSVAAAHVPPVVNP
                     RASRDYIKAKAAAMTGHEAPAAGRAAGGDAHGGDVHWAYEGENGPQAWGKLKPEFNMC
                     AIGKRQSPINIEDSGTLQGPAEPLQFSYTPSSGTVVNNGHTIQVDLQGDNSLTVRGSV
                     YRLLQFHFHTPSEEQVNFRNFAMVAHLVHKNSEGQLAVVAVLLDPGVVNALINKVWTY
                     MPLDQSDRVRLPAGIIDMNELLPKDQRYYQFIGSLTTPPCT"
     gene            1261..2319
                     /gene="ruvB"
                     /locus_tag="PKX82_04950"
     CDS             1261..2319
                     /gene="ruvB"
                     /locus_tag="PKX82_04950"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOF51631.1"
                     /translation="MSIQTDDFAPAPPKRVVSNAPASPNEEAIERALRPKLLEDYVGQ
                     VKAREQLEIFISAAKMRGEALDHVLLFGPPGLGKTTLSHIVAHELGVNLRQTSGPVLE
                     KPKDLAALLTNLEKNDVLFIDEIHRLSPVVEEILYPALEDYKIDIMIGEGPAARSIKL
                     DLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTSEELGRIVKRSAGLLNVPMDAEG
                     GFEIARRSRGTPRIANRLLRRVRDYAEVKGVGTITLDMANRALAMLDVDPQGFDLMDR
                     KLLEAVIHRFDGGPVGLDNIAASIGEERETIEDVIEPYLIQQGYLQRTPRGRIATLAA
                     YRHLGVTPKSTGEGLFAE"
     gene            2327..3076
                     /locus_tag="PKX82_04955"
     CDS             2327..3076
                     /locus_tag="PKX82_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOF51632.1"
                     /translation="MNTVMLRTLSALAVAASLLASARAADLTAYTVDWAPYNYSEQGA
                     IKGFSTDMLRAMCQSAKIDCDIRMVPWARAYKTVQTTPNTLVFTTARKPSRENEFLWV
                     GPIVPRSTWVYGKTGQEKSVRILQDLAQLRIGVVRGEAAQQDLLDAGVPASSLVEEAT
                     KAEVVRLLVGARVDAMVETELGMAWHLRSASIPATTVTKLVKLSDGGAYYYALNLQTD
                     PALVKKLQDALDKLRRTGKLQPIIAKYTAAK"
     gene            complement(3092..4843)
                     /locus_tag="PKX82_04960"
     CDS             complement(3092..4843)
                     /locus_tag="PKX82_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HOF51633.1"
                     /translation="MSPYQVIVLATPVFLLLIALEFAWSRTRASRTAGQQPYRLNDAI
                     NSISLGIISQLFGVLSKVLSIGIYTLVFGAVAIYPDLAFWTTWYGIVLALIFYDFCYY
                     WVHRAGHVIALFWAAHVVHHQSQHYNLSTALRQTSSGALFGWIFYIPMAIAGVPPLVF
                     GIVALIDLLYQFWVHTEQVGKLGWFDRVFCSPSNHRVHHAVNDPYIDKNYGGLLVIWD
                     RMFGSFQEEMDHEPCVYGTRGALNSWDPVWANLEVYWALAKDSWATRNWGDKVRIWFK
                     PPGWQPEDLKQAHPKPAFKLENVTTYNPPLTAGQQWFAALQFILALAAVSVFLWNADT
                     MSMGTAALWIAAISASMWALGLFMQGRLALLEVLVLESAALATLSALGMVDLHLVFKP
                     ATMAIAIIFVATRAISTGAIGRFDALLLAALVFSLGGDVFLMLPGDQPLWGLPTFILG
                     LGSFLVAHLFYIVLFRQGQPWFPSKRALLVVLGVGAAMYAIVWGGLGNDTVLKIAVAA
                     YVTVISLMTAQAIGRAVTLGDSASRWVAVGACVFMVSDSLIAINKFVTPVALSSLWIL
                     VTYYCAQMLIVHHSRKA"
     gene            complement(4900..5550)
                     /locus_tag="PKX82_04965"
     CDS             complement(4900..5550)
                     /locus_tag="PKX82_04965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOF51634.1"
                     /translation="MGATRIIAVRHGETAWNVDTRIQGQLDIDLNDKGRWQAQRVAQA
                     LADEPIHRVYSSDLSRAHATARAIAQHASEPTAREVQLHTGLRERGFGTFEGQTWADI
                     ETQWPEESRRWRQRDPHFAPPGGETLVQLRERIAGAVAALAAQHLGQQIVLVAHGGVM
                     DMLYRLATSQDIGAPRTWELGNAAINRLLWTPDALTLVGWADTRHLEDETLDESST"
     gene            complement(5550..6143)
                     /locus_tag="PKX82_04970"
     CDS             complement(5550..6143)
                     /locus_tag="PKX82_04970"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HOF51635.1"
                     /translation="MRRAALWLMSACLLPLLLAGCAMPRMIDSDVNSFAGTAPAVRGA
                     SFRFDRLPSQQNSGEVQDRIEAIAQTALERVNLMRHDAAPRYLVQVQVGAETMRNPYY
                     RPRTRLVVGADGRLYEEWPLFPDLDTLWFRHSVKMVLRDSANGQAAYETTAVFDGPWS
                     DTLNLLPAMLEAALQDYPQPMRRRVVVELPAPTGGNR"
     gene            complement(6147..>6579)
                     /locus_tag="PKX82_04975"
     CDS             complement(6147..>6579)
                     /locus_tag="PKX82_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOF51636.1"
                     /translation="TFFLNMFFGGKLKGMPPKLTSDNGEFIVIRPMAYVAEKDLIRWA
                     EHRQFPIIPCSLCGSQENLQRVQAGKMLREWEKQYPGRAETMFTALQNVVPSHLMDSK
                     LHDFKNLKTTGIPDENGDKAFDEEEFPTAVPSLPGLQVIQV"
BASE COUNT         1202 a         2003 c         2175 g         1199 t
ORIGIN      
        1 cggtgcacgg tggcgtggtc agcgagccaa tgaactggta atagcgctgg tctttgggca
       61 acaactcgtt catgtcgatg atgccggcag gcaggcgcac ccggtcactt tggtccagtg
      121 gcatgtaggt ccacaccttg ttgatcagcg cattgaccac gccagggtca agcaatacgg
      181 ccaccaccgc cagttggcct tcagagttct tgtgcaccag atgcgccacc atggcgaagt
      241 tgcggaagtt gacctgctcc tctgacggtg tatgaaaatg gaactgcagc agcctgtaaa
      301 ccgagccacg caccgtgagc gagttgtcgc cctgcaggtc gacttgaatc gtgtggccat
      361 tgttgaccac ggtgccgctg gagggcgtgt agctgaactg cagcggttcg gccgggcctt
      421 gcagggtgcc ggaatcttca atgttgatgg gtgattggcg cttgccaatg gcacacatat
      481 tgaactcggg tttgagcttg ccccaggcct ggggtccgtt ttcgccttca taggcccaat
      541 gaacgtcgcc cccatgggca tcaccgcctg ctgctcgacc cgcagcaggc gcctcatggc
      601 cggtcatggc cgcagccttg gccttgatgt agtcgcggct ggcgcgcggg ttgaccaccg
      661 ggggcacatg ggcagccgcg acactgtgcg ctgccacggc tggcgccaca ggcgccatgg
      721 ctcggctgct gggcggtgcc acatgctcgg gctccttgat actggctgcg gcgggtttgg
      781 ctggcgggtt atctttgatc accagcgtca ggctcttctt gtcgatgagt tggtccttga
      841 gcgcgctgcg caggtcaccc gccagggatt tgccctcagc tggctcggcc gagcttgctt
      901 gcaccggttt ggctgcagag gcttttgtgg ccgccactgg cttgggtgtg ccgtggtcgg
      961 cggtcgcgcc atgttcggcg gtggcagcgt ggctctttgg caaggttgcc ccgatcagtg
     1021 ccagggccag gcagcagggc aaggccaggt ggtgccagcg aaacccggaa ttgcagatcg
     1081 ccgactggcg tgaggcgggg gcggggaagg gacttggtgt tttcatgtcg tggccggggc
     1141 caggcggtga cccgagaaaa tcttttatga catgttatcg gcagattgca cgatggtctt
     1201 gagccggggc gccccgcgtg ggcgctatag tgccagcatg tccctgcaac aaagcctcct
     1261 gtgagtattc aaaccgacga ctttgcgccc gccccgccca aacgcgtggt gtccaacgcc
     1321 cccgcctcgc ccaatgagga ggccatagag cgcgctctgc gccccaagtt gctggaagac
     1381 tatgtgggtc aggtcaaggc gcgcgagcag cttgaaatat tcatcagtgc ggccaagatg
     1441 cggggcgaag cacttgacca tgtattgctg tttggtccgc ccggcctggg caagacaacg
     1501 ttgtcacaca tcgttgcgca tgaactgggc gtgaacctgc ggcaaaccag tgggccagtg
     1561 ctggaaaagc ccaaggacct ggcggcgctg ctgaccaatc tggaaaagaa cgacgttctc
     1621 ttcatcgacg aaatccaccg cctcagcccg gtggtggaag aaatcctgta ccccgcgctg
     1681 gaagactaca agatcgacat catgatcggc gagggcccgg cggcccgctc catcaagctc
     1741 gacctgcagc ccttcaccct ggtgggcgcc accacccgtg ctggcatgtt gaccaacccg
     1801 ctgcgcgacc gttttggcat cgtggcgcgg ctggagttct atacctccga agagctgggc
     1861 cgcatcgtca agcgcagtgc gggcctgctc aatgtgccga tggatgcgga gggtggtttc
     1921 gagatcgccc gccgttcgcg cggtacaccg cgcattgcca atcgcctgct gcgccgggtg
     1981 cgcgactatg ccgaggtcaa gggcgtgggc accatcaccc tggacatggc caaccgcgcg
     2041 ctggccatgc tggatgtgga cccgcagggg tttgacctga tggaccgtaa gctgctcgaa
     2101 gccgtgatcc accgctttga cggtggcccg gtcgggctgg acaacatcgc ggccagcatt
     2161 ggcgaagagc gcgaaaccat cgaagatgtg atcgagccct acctgatcca gcagggttac
     2221 ctgcaacgca ccccgcgcgg ccgcatcgcc acactggcgg cctaccggca tctgggggtg
     2281 acacccaaga gcacgggtga aggcctgttt gccgagtgaa cacaccatga acactgtgat
     2341 gcttcggaca ttgtctgcac tggcggttgc tgccagtctg ctggcgtctg cccgcgcagc
     2401 cgatctcacg gcctataccg tggactgggc gccttacaac tattcggagc agggtgccat
     2461 caagggcttt tcgacggaca tgttgcgggc catgtgccag agcgcaaaga tcgattgcga
     2521 catccgcatg gttccctggg cgcgtgccta caaaacggtc cagaccacgc ccaacacact
     2581 ggtgttcacg accgcacgca agccgtcgcg cgagaacgaa tttttgtggg tggggcccat
     2641 cgtgccgcgc tccacctggg tgtatggcaa gacagggcag gaaaagtcgg tccgcatcct
     2701 gcaagatctg gcgcagttgc gcattggcgt ggtgcgtgga gaggccgcgc agcaggacct
     2761 gctggatgcc ggggtgccag cgtcgtccct ggtggaagag gcgaccaaag ccgaggtcgt
     2821 gcgtttgctg gtgggtgccc gggtggatgc catggtcgag accgagctgg gcatggcatg
     2881 gcatctgcgc agtgcctcca ttccagccac tacggtgacc aagctggtga aactcagcga
     2941 tggcggtgcc tactactacg ccctgaatct ccagaccgat ccggccctgg tcaaaaaact
     3001 gcaggacgca ctggacaagc tgcggcgcac aggcaaattg cagcccatca tcgcgaaata
     3061 cacggctgca aaataacggt gcggatggcc ctcaggcctt gcggctgtgg tgcacgatca
     3121 gcatctgcgc gcagtagtag gtcaccagaa tccacagcga cgacagcgcc accggcgtga
     3181 caaacttgtt gatcgcaatc agtgagtcgc tcaccatgaa tacacaggcg cccaccgcca
     3241 cccagcgtga ggcgctgtcg cccagcgtga cggcgcggcc gatggcctgt gccgtcatca
     3301 gcgagatgac ggtcacgtag gccgccacgg caatcttgag caccgtatcg ttgcccagcc
     3361 caccccaaac gatggcgtac atggctgccc caacgcccag caccaccagc agggcgcgct
     3421 tgctgggaaa ccagggctgg ccctggcgga acagcacgat gtagaacagg tgggccacca
     3481 aaaacgaccc caggcccaga atgaaggtgg gcaaccccca cagaggttgg tcacctggca
     3541 gcatcaggaa cacgtcgcca cccagcgaaa acaccagcgc agccaacaat aaggcatcaa
     3601 atcggcctat agcccccgta gatattgcgc gagtagctac aaaaataata gcaattgcca
     3661 tggtggcggg cttgaacacc aggtgcaggt ccaccatacc cagcgcactt agcgtggcca
     3721 gggcggcgct ttccagcacc agaacttcga gcagggccag ccgaccctgc atgaacaggc
     3781 ccagcgccca catggaggcg ctgatggccg caatccagag tgctgcggtt cccatggaca
     3841 tggtgtcggc gttccacagg aagaccgata cggccgccag tgccaggatg aactgcagcg
     3901 cggcaaacca ctgctgcccg gcggtcagcg gtgggttgta ggtggtgacg ttttcaagct
     3961 tgaacgcggg tttggggtgg gcctgcttca ggtcctcggg ctgccagccg ggcggtttga
     4021 accagatgcg caccttgtcg ccccagttgc gcgtggccca ggagtccttg gccagcgccc
     4081 agtacacctc caggttggcc cacaccgggt cccagctgtt gagtgcgccg cgggtgccgt
     4141 acacgcaggg ttcgtggtcc atctcttcct ggaagctgcc gaacatgcgg tcccagatga
     4201 ccagcaggcc gccgtagttt ttgtcgatgt acgggtcgtt caccgcgtgg tgcacgcggt
     4261 ggttgctggg ggagcagaac acccggtcaa accagcccag cttgcccacc tgctcggtgt
     4321 gcacccagaa ctggtagagc aggtcgatca gcgccacgat gccaaacacc agcggtggca
     4381 cgccggcaat cgccatgggg atgtagaaga tccagccaaa cagggcgccg ctgctggtct
     4441 ggcgcaacgc ggtcgacagg ttgtagtgct ggctctggtg gtgcaccacg tgggccgccc
     4501 agaacagggc aatgacgtgg cccgcgcggt ggacccagta gtagcagaag tcgtaaaaaa
     4561 tcagcgccag cacgatgccg taccaggtgg tccagaacgc caggtccggg tagatggcca
     4621 cggcaccgaa cacgagggtg taaatgccga tgctcaggac cttgctgagt acaccgaaga
     4681 gctggctgat gatgcccagg ctgatgctgt tgatggcgtc attgaggcgg tagggttgct
     4741 ggcccgcagt gcggctggcg cgcgtgcggc tccaggcaaa ttccagtgca atcagcagca
     4801 gaaaaacggg ggtggcgagg acgatgactt ggtaggggct catgccctga ttttcgccga
     4861 gcaagcgctt tgtgaaacgc gagtgaaccc tagggacccc taggtgctgg attcgtccag
     4921 cgtttcgtct tccaggtgcc gggtgtcggc ccaacccacc aaagtgagtg cgtcgggcgt
     4981 ccacagcagg cggttgatgg cggcgttgcc cagctcccag gtgcgcgggg caccaatgtc
     5041 ctggctggtg gccaggcggt agagcatgtc catcacccca ccgtgggcga ccagcacgat
     5101 ctgctgcccc aggtgctgcg cagccaaggc cgccaccgcg ccggcgatgc gctcgcgcag
     5161 ttgcaccagg gtttcaccgc cgggcggggc aaagtgcggg tcgcgctggc gccagcggcg
     5221 ggattcttcg ggccattggg tttcaatgtc tgcccaggtc tggccctcaa acgtgccaaa
     5281 gccgcgctcg cgcaggccgg tatgcaactg cacttccctt gccgtgggct cgctggcgtg
     5341 ctgcgcaatg gctcgggccg tggcatgggc gcgcgacagg tcactggagt acacgcggtg
     5401 gatgggctca tcggccagcg cctgcgccac acgctgggct tgccagcgcc ctttgtcgtt
     5461 gaggtcgatg tcgagctggc cctggatgcg ggtgtccacg ttccaggcgg tttcaccgtg
     5521 gcgcacggcg atgatgcggg tggcgcccat cagcggttgc ctcccgtggg ggcgggcagt
     5581 tccaccacca cgcggcggcg catcggctgc gggtagtcct gcagggctgc ctccagcatg
     5641 gcgggcagca ggttcagtgt gtccgaccag gggccgtcaa atacagcggt ggtctcgtaa
     5701 gcggcctggc cgttggccga gtcgcgcagc accatcttga cgctgtggcg aaaccacagt
     5761 gtgtccaggt ccgggaacag cggccactct tcgtagagcc ggccatcggc accgacgacc
     5821 agccgggtgc gcgggcggta gtaggggttg cgcatggttt cagcccccac ttgcacctgc
     5881 accaggtagc gcggcgcggc atcgtggcgc atcaggttca cacgctccag cgccgtctgg
     5941 gcgatggcct cgatccggtc ttgcacctcg ccagagttct gctgcgaggg aagccggtca
     6001 aaccggaagc tggcgccgcg caccgcgggt gcggtgccgg caaagctgtt gacgtcgctg
     6061 tcgatcatgc gcggcatcgc gcagccggcc agcagcaggg gcaacaggca ggcagacatg
     6121 agccagagtg cagcgcgtcg catggcttac acctggatca cttgcagacc cggcaggctg
     6181 ggcactgcgg tggggaactc ttcctcgtca aacgccttgt ctccgttttc atcgggaatg
     6241 ccggtggtct tgaggttctt gaagtcgtgc agtttgctgt ccatcaggtg gctgggcacc
     6301 acgttctgca gcgcggtgaa catggtctcg gcgcggcccg ggtactgctt ttcccactcg
     6361 cgcagcatct tgccggcctg cacgcgctgc aggttttcct ggctgccgca caggctgcag
     6421 gggatgatgg ggaactggcg gtgttcggcc cagcggatca ggtctttctc ggccacatag
     6481 gccatgggcc ggatgacgat gaactcgccg ttgtcgctgg tgagcttggg cggcatgccc
     6541 ttgagcttgc cgccaaagaa catgttcaaa aagaaggtc
//
LOCUS       DAOSJM010000100         6572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10647_length_6572_cov_3.995704, whole genome shotgun sequence.
ACCESSION   DAOSJM010000100 DAOSJM010000000
VERSION     DAOSJM010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6572
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10647_length_6572_cov_3.995704"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PKX82_04980"
     CDS             <1..934
                     /locus_tag="PKX82_04980"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOF51637.1"
                     /translation="PDQVAALDDFCPHRGAPLSLGTVCEGHLQCGYHGLRVECTGKSV
                     SMPGQRVQGFPKAKSFPVVERYGFIWVWPGDADKADPATIHHLPWANNSEWAYGGGLY
                     HIACDYRLMIDNLMDLTHETYVHSTSIGQKEIDETPCKTTVDGDMVTTARYMSGITPP
                     PFWKAALRSHGLPDDVTVDRWQICRFTPPSHVIIEVGVAMEGQGGYDAPNDKKVYSKV
                     IDFITPETETSIHYFWGMARKFNPKDKALTATIREGQGKIFGEDLEMLERQQRNLIDY
                     PEKKLLMLNIDAGGVQSRKIIDRLIAAEQAGTAA"
     gene            931..1914
                     /locus_tag="PKX82_04985"
     CDS             931..1914
                     /locus_tag="PKX82_04985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HOF51638.1"
                     /translation="MSTAQTLQVRVAAKSVEALDIVTLELVAVDGKPLPAFSAGSHVD
                     VMLPGDQGVPDITRQYSLCNDPKETHRYQIAVLRDPASRGGSVAVHDRVNAGDVLQIS
                     APKNHFGLAHDAKKHLLLAGGIGVTPILCMAERLAQTGADFQMHYATRAPERTAFRAR
                     IAASGFADRVQFHYDNAEAAQKLDLQGLLAKPSQGTHLYVCGPKGFMDAVLGSARAQG
                     WPEGQLHYEFFGAAVASSDSDAGFEVKLASSGRIIQIAKDKTVTKALAEAGVDILMSC
                     EQGVCGTCLTRVLEGTPDHKDSYLTPEERAANDQFLPCCSRSKTPMLVLDL"
     gene            1952..2791
                     /gene="pyrF"
                     /locus_tag="PKX82_04990"
     CDS             1952..2791
                     /gene="pyrF"
                     /locus_tag="PKX82_04990"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOF51639.1"
                     /translation="MTFIDMLGAAERQNQSLLCVGLDPEPAKFPGALQGDASKIYDFC
                     AAIVDATADLVIAFKPQIAYFAAHRAEDQLEKLMEHMRRTAPQVPVILDAKRGDIGST
                     AEQYAKEAFERYGADAVTLSPFMGFDSVQPYLKYHGKGAFLLCRTSNPGGDDFQPQRL
                     LDVPGQPRLYEHIAALAQGPWNLNGQLGLVVGATYPAEIEQVRAVAPTLPLLIPGVGA
                     QGGDAVATVKAGYRSAGGKTTAPIVVNSSRAVLYASQGADFAQAARAAAMATRDQLQA
                     AKP"
     gene            2874..3584
                     /locus_tag="PKX82_04995"
     CDS             2874..3584
                     /locus_tag="PKX82_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HOF51640.1"
                     /translation="MIDLYTAATPNGHKVSIALEELALSYTLKTLDLAKGEQKLPAFL
                     AINPNGRIPAIVDRAEDDFAVFESGACLIYLAEKTGQLMPTDVKGRSRVIQWLMFQMG
                     GIGPMMGQANVFYRYFPEKIQPAIDRYQGESKRLFRVLDGHLKDHEYLAGDYSIADIA
                     NWAWVRTHRWSGVEVDDLPHLQRWLAAIRARPAVQKGIEMPPSKMDLTRDGEDKAKEF
                     SEQARTMVEMGQSRKPGV"
     gene            3587..4207
                     /locus_tag="PKX82_05000"
     CDS             3587..4207
                     /locus_tag="PKX82_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOF51641.1"
                     /translation="MKLYVAPRAPSPRRVMMFMVEKNITGIDLVNVDLNAQEHKGEAY
                     RAKSPLAKVPALELDDGRVLTETRAICTYLEGLVPEPNLMGVDAEERAFIEMADRRVE
                     HYLLAGIANAVRHTHPGLAPLEQPQFPDYGHSQAQKVREIAQWFDHVLEHQPWMAGDR
                     FTIADITAFCAIEFAKLMKFSPGHEGMLALQAWRDRVADRPSARVQ"
     gene            4361..6169
                     /locus_tag="PKX82_05005"
     CDS             4361..6169
                     /locus_tag="PKX82_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HOF51642.1"
                     /translation="METEVLDQIEVPQGLPTEATAQDTDTLSAAKLAAVAAAAALAAC
                     GGGGGGSGGAAPYVAVPVPGRTVNLSAGGYTYPTATSDEQAARFLLQAQFAATDADIA
                     SVRSLGYARWLERQIATSWTTGWDWLMVQGYGDVQSSNNYYDRSEQTDFMVWNQLITA
                     PDPFRKRAALALSEFFVVSTNGVSATWVSHIMAHYWDTLCRHAMGNYRDLLEAITLNV
                     AMGYYLNTKGNLKENAYGRQPDENYAREVMQLFTIGLVELNTDGTQKTFAGEPIDSYG
                     PSDVSNLARVFTGYDLDQSQNVNTVISGRTVPSTHFARLPMKQVGNNHSTLAATFLGV
                     TIPANTSASAALKTALDTLFNHANTAPFFCKQMIQRLVTSNPSRDYVQRVVNKFLDNG
                     QGVRGDLAHVFSAILMDDEARGPAGLTDTEFGKLREPMVRMVQWARTFWVTSTSGAWR
                     ISDLSDAGSRLSQSPLRSPSVFNFFRPGYVAPGTTLSVGKVAPEFQLVNESSVGGYLN
                     YMMGVISNGISSDMKAVYTAEIALANTSGALVQRINLLLCAGQLSSTTVSLIANAVMT
                     MPGVVTDTTTTATNMRNRVYATVLLTMACAEYLIQK"
     gene            6181..>6572
                     /locus_tag="PKX82_05010"
     CDS             6181..>6572
                     /locus_tag="PKX82_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOF51643.1"
                     /translation="MHSSDIPSPSRRAFLRRSGHLALTGTALPFALNLAAMGEAAAFN
                     STAGDYKALVCVFMFGGNDYGNTVIPYDDTSYDKYHTIRGGGATGQGAAGIAIAKSAL
                     APTLLAPTTAPLDVVSGQPRQYALHPAMT"
BASE COUNT         1298 a         2076 c         2049 g         1149 t
ORIGIN      
        1 gcccgatcag gtcgccgcgc tggacgactt ctgcccccac cggggcgcac cgctgtcgct
       61 gggcacggtg tgcgaaggcc atctgcaatg cggctaccac ggcctgcggg tggaatgcac
      121 gggcaagtcg gtgtccatgc ccggtcagcg cgtgcaaggg ttccccaagg cgaagagctt
      181 tccggtggtg gagcgctacg gcttcatctg ggtgtggccc ggcgacgccg acaaggccga
      241 ccctgccacc atccaccacc tgccctgggc caacaactcc gaatgggcct atggcggcgg
      301 gctgtaccac attgcctgcg actaccgcct gatgatcgac aacctgatgg acctgacgca
      361 cgagacctat gtgcactcca ccagcatcgg ccagaaggaa attgacgaaa ccccatgcaa
      421 gaccacggtg gacggcgaca tggtgactac cgcccggtac atgagcggca tcacaccgcc
      481 acccttctgg aaggccgcgc tgcgcagcca cggcctgccc gatgacgtca cggtcgaccg
      541 ctggcagatt tgccgcttca cgccgcccag ccacgtcatc atcgaagtcg gcgtggcgat
      601 ggaaggccag ggcggctacg acgcgcccaa cgacaagaag gtctacagca aggtgatcga
      661 cttcattacg cctgaaaccg aaacctcgat ccactacttc tggggcatgg cgcgcaagtt
      721 caaccccaaa gacaaggcgc tgaccgcgac catccgcgaa ggccagggca agatctttgg
      781 tgaggacctg gagatgctgg agcgccagca acgcaacctg atcgactacc ccgagaagaa
      841 gctgctgatg ctcaatatcg acgccggtgg cgtgcagtcg cgcaagatca ttgaccgtct
      901 catcgccgct gaacaggctg gcaccgcagc atgagtactg cgcagactct gcaggtgcgc
      961 gtggcggcca agtccgttga ggcgctggac atcgtgacgc tggagctggt ggccgtggac
     1021 ggcaagccgc tgcccgcgtt ctcggccggt tcgcatgtgg acgtcatgct gccgggcgac
     1081 cagggcgtgc cggacatcac gcgccagtat tcactgtgca acgacccgaa ggaaacgcac
     1141 cgttaccaga tcgctgtgct gcgtgacccg gcttcgcgcg gtggttcggt ggcggtgcac
     1201 gaccgggtga atgcaggcga tgtgctgcag atcagcgctc cgaagaacca ttttggactg
     1261 gcgcacgatg cgaagaagca cctgctgctg gccggtggca ttggcgttac gcccattctg
     1321 tgcatggccg agcgactggc ccagaccggc gccgacttcc agatgcacta cgccacgcgc
     1381 gcaccggaac gaaccgcctt ccgcgcgcgc attgcggcct cgggttttgc ggatcgggtg
     1441 caattccatt acgacaacgc agaagccgca caaaagctgg acctgcaggg gctgctggca
     1501 aaaccgtcgc agggaacgca cctctacgtc tgcggaccta agggttttat ggatgcggtg
     1561 ctgggctcgg cgcgtgcgca aggctggccc gaaggccagc tgcactatga gttcttcggt
     1621 gcggcggtgg cgtcatcgga cagcgatgca ggctttgaag tgaagctggc cagctccggc
     1681 cgcatcatcc agatcgccaa agacaaaacg gtgaccaagg cgctggccga ggctggtgtg
     1741 gacatcctga tgtcgtgcga gcagggcgtg tgcggcacct gcctgacccg tgttctggaa
     1801 ggcacgccgg accacaagga cagctacctg acccctgagg agcgcgccgc caacgaccag
     1861 ttcctgccct gttgctcacg ctccaaaacg ccaatgctgg tgctggacct gtaaagcgca
     1921 gcaggggcgc gcaggccgct aaacttgcgc catgaccttt atcgacatgc tgggcgcagc
     1981 agagcgccaa aaccaatccc tgctgtgtgt ggggctggac cccgaacccg ccaaattccc
     2041 aggcgccctc cagggcgacg ccagcaagat ctatgacttc tgcgcagcca tcgtcgatgc
     2101 cacggccgac ctggtcattg ccttcaagcc gcagattgcc tactttgccg cccaccgcgc
     2161 cgaagaccag ctggaaaagc tgatggaaca catgcgccgc accgcgcccc aggtgccggt
     2221 cattctggat gccaagcgcg gcgacatcgg cagcaccgcc gagcaatacg ccaaagaggc
     2281 ttttgagcgc tatggcgcgg atgcggtgac gttgtcgccc ttcatggggt ttgactcggt
     2341 gcaaccctac ctcaagtacc acggcaaagg cgccttcctg ttgtgccgca cgtcgaaccc
     2401 cggtggcgac gacttccagc cccagcgtct gctggatgtg cccggccagc cacggctgta
     2461 cgaacacatt gccgcgctgg cccaggggcc ctggaacctg aacggccagc tcggcctggt
     2521 ggtgggggcc acctaccccg ccgaaattga acaggtgcgt gcggtggcgc cgacgctgcc
     2581 gctgctgatt cccggcgtgg gcgcgcaagg tggcgatgcg gtggccacgg tgaaggctgg
     2641 ctaccgcagt gcgggtggaa agaccacggc acctatcgtg gtgaattctt cgcgtgccgt
     2701 gctgtatgcc tcgcagggtg cagacttcgc gcaggcggcc cgcgctgcgg ccatggcaac
     2761 gcgtgatcag ctgcaggcag cgaagccgta accgctataa aattgatagc tgcttgcgcg
     2821 caatgcacgg gggctagggg ccaatttagc ttgaaatcct gaaagacaac cacatgattg
     2881 acctctatac cgccgccacg cccaacgggc acaaggtgtc catcgccctg gaagagctgg
     2941 cactgtctta caccctgaaa acgctggacc tcgccaaggg cgagcagaag cttcccgcat
     3001 ttctggcgat caaccccaac ggacgcatcc ccgcgatcgt ggaccgggcc gaggacgact
     3061 ttgcggtgtt cgaatccggc gcctgtctga tctacctggc cgagaagact ggccagctga
     3121 tgcccacgga cgtgaaaggc cgctcgcgcg tgatccagtg gctgatgttc cagatgggcg
     3181 gcatcgggcc catgatgggc caggccaatg tgttctatcg ttactttccc gagaagatcc
     3241 agcccgccat cgaccgctac cagggcgaaa gcaagcggct gttccgcgtg ctcgatggcc
     3301 acctgaaaga ccacgaatac ctggccggcg actattcgat tgccgacatt gccaactggg
     3361 cctgggtgcg cacccaccgc tggtcgggcg tggaggtgga cgatctgccg cacctgcagc
     3421 gctggctcgc agccattcgc gctcgccccg cagtgcaaaa gggcatcgag atgccgcctt
     3481 ccaaaatgga cctcacccgt gacggcgagg acaaggccaa ggagttttca gaacaggccc
     3541 gcaccatggt ggagatgggc cagtcacgca aaccaggagt ctgacgatga agctgtatgt
     3601 ggcaccccgg gcaccgagcc cacggcgcgt gatgatgttc atggtggaaa agaacatcac
     3661 cggtatcgat ctggtcaacg tcgacctgaa tgcgcaggaa cacaagggcg aggcttaccg
     3721 cgccaagagc cccttggcca aggtgcccgc gctcgaactc gatgacgggc gcgtgctgac
     3781 cgagacacgc gccatttgca cctacctgga aggcctggtc cccgaaccca acctgatggg
     3841 cgtggatgcg gaagagcgcg cctttatcga gatggccgac cgccgggtcg agcattacct
     3901 gctggcgggc attgccaatg cggtgcgcca cacccacccg ggactggcgc cgctggagca
     3961 gccccagttt ccggactatg gccattcgca ggcgcagaag gtgcgcgaga ttgcgcagtg
     4021 gtttgaccat gtgctggagc accagccctg gatggccggg gaccggttca ccattgccga
     4081 catcaccgcg ttctgtgcca tcgaatttgc caagctgatg aaattcagcc cgggccacga
     4141 gggcatgctg gcattgcagg cctggcgtga ccgcgtggca gaccggccca gcgccagggt
     4201 gcaatagccg ccgctggtaa gcccccggta agcctcggta acaggacccg ttacctgggg
     4261 taattgtttg taagaccgct tggatatgcg gcctgcgctg gctaacctcc agcccgttgg
     4321 ttcaacctgg gggttggacc cgttcagggg gttatccaag gtggaaacag aagtactaga
     4381 ccagatagag gtgccacagg gcttgccgac tgaggcgacg gcgcaggaca ccgacacact
     4441 gtccgcggcg aagctggcgg ccgtcgcggc tgcagcggca ctggctgcgt gcggcggcgg
     4501 aggtggcggc agtggtggtg cggcgcccta tgtggccgta ccggtgccag ggcgcacggt
     4561 caacctctcc gccggggggt atacctatcc caccgcgacc tccgacgagc aggccgcacg
     4621 ctttcttttg caggcccagt tcgcagcgac ggatgcggac atcgcgtcgg tgcgcagtct
     4681 gggctatgcg cgctggctgg agcgccagat agccacctcc tggaccactg gttgggactg
     4741 gctcatggtg cagggctatg gcgatgtaca gagcagcaat aactactacg accgcagcga
     4801 acagaccgat ttcatggtct ggaaccagtt gataacggca cccgaccctt ttcgcaagcg
     4861 ggctgcgctg gcgctgtcag agttcttcgt ggtgtcgacc aatggcgtga gtgccacctg
     4921 ggtcagccac atcatggcgc actactggga taccctgtgc cgccatgcca tgggcaacta
     4981 ccgcgaccta ctggaagcca ttaccctgaa tgtggcgatg gggtactacc tcaacaccaa
     5041 aggcaacctc aaggagaacg cctacgggcg ccagccggac gagaactatg cacgcgaggt
     5101 catgcagctc ttcacgatcg ggctggtgga gctcaacacc gatggcaccc aaaagacatt
     5161 tgccggtgag ccgatcgaca gctatggccc cagcgatgtg agcaatctgg cacgggtgtt
     5221 caccgggtac gacctggacc agagccagaa tgtcaatacg gtcatcagtg ggcgcaccgt
     5281 tccgagtacc cactttgcgc gcttgccgat gaagcaggtc ggtaacaacc attccacact
     5341 ggcagccacg tttctaggcg tcacgatccc tgcgaataca tcggcgtcag cggcgctcaa
     5401 gacggcgctg gacacgctgt tcaaccatgc caataccgcg cctttcttct gcaagcagat
     5461 gatccagcgc ctggtgacca gcaatcccag ccgggactat gtgcagcgtg tggtgaacaa
     5521 gttcctcgat aacggccagg gtgttcgtgg tgatctggcc cacgtgtttt ccgcgatcct
     5581 gatggacgat gaagcccgtg gtccggcggg cctgaccgat accgaatttg gcaaattgcg
     5641 cgagcccatg gtgcgcatgg tgcaatgggc ccgcaccttt tgggtgacct caaccagcgg
     5701 tgcctggagg atcagcgacc tgagcgacgc aggatcgcga ctgagccaaa gtccactgcg
     5761 ctcaccttcc gtgtttaatt tcttccggcc tggttatgtg gcaccgggga ccactctgtc
     5821 tgttggcaaa gtggcgccgg agttccagct ggtcaacgag agtagcgtgg gcggctatct
     5881 caactacatg atgggtgtga tcagcaacgg catcagttca gatatgaagg cagtctatac
     5941 cgccgaaatc gcgctcgcca acacatctgg cgcgctcgtg caacgcatca acctgctgct
     6001 atgcgccggc cagctttcca gcaccacggt gtcgctgatc gcgaatgcgg tgatgaccat
     6061 gccgggtgtg gtgaccgata ccaccaccac ggccaccaat atgcgcaacc gcgtgtacgc
     6121 cacggtgctg ctgaccatgg cctgcgccga atacctgatc cagaaatagc gagacgaacc
     6181 atgcattctt ctgatatccc atccccttcg cgtcgcgcat ttctccgccg ttccggtcac
     6241 ctggcgttga cgggtaccgc gctgcccttt gccctgaacc tcgccgcgat gggcgaggcc
     6301 gcggctttca actccacagc gggggactac aaggcgctgg tctgtgtgtt catgttcggt
     6361 ggcaacgatt acggcaacac cgtgatcccg tacgacgaca ccagttacga caaataccac
     6421 acgatccgcg gtggtggcgc caccggccag ggcgcagcgg gcattgccat tgccaagtcg
     6481 gcgctggcgc ctaccttgtt ggcacccacg acggctccgc tggacgtggt ttccggccaa
     6541 ccacgtcagt acgcgctgca ccctgccatg ac
//
LOCUS       DAOSJM010000101         6565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10668_length_6565_cov_4.229339, whole genome shotgun sequence.
ACCESSION   DAOSJM010000101 DAOSJM010000000
VERSION     DAOSJM010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10668_length_6565_cov_4.229339"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PKX82_05015"
     CDS             <1..443
                     /locus_tag="PKX82_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51644.1"
                     /translation="AQLVRDALSTAASQGWTEMVWSDPNFEDWPLRERVVVDALQAWA
                     GRGRKLVMLAHSFDSVIRFQPRFVQWRTTWDHVVECRVCRQLDASEVPSALWSPHWAM
                     RRLDLARSTGMAGSEPQRRVLLRETLEECRRNSAPGFPSTVLGL"
     gene            complement(877..1959)
                     /gene="bamC"
                     /locus_tag="PKX82_05020"
     CDS             complement(877..1959)
                     /gene="bamC"
                     /locus_tag="PKX82_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HOF51645.1"
                     /translation="MNSISKLGLTAVALALSACTALETDKVDYRSTGKAPSLTVPPDL
                     TQLSKDTRYTVVDGGVSASGYQSGQASASKTPVAAVALGDVRIERAGNQRWLVVKRPA
                     ETLWEPVKDFWQENGFLLAMDQSSLGIMETDWAENRAKIPQDAIRAALGKLVDALYST
                     AERDKFRTRLERNASGETEIFISHRGVIEVYTSEQKRDTVWQPRPTDPELEAEFLRRL
                     MVKLGVSPEQSKALVANSVAKSVATVSSTNGQPVVLIEENFDRAWRRVGLALDRTGFT
                     VEDRDRKQGIYFVRYVAPGSENSEPGFFSKIFSGSSKTIEPTKYQIAVRSQGDATTVL
                     ILDAKGGPESSANAQRIVKVIADDIR"
     gene            complement(1986..2879)
                     /gene="dapA"
                     /locus_tag="PKX82_05025"
     CDS             complement(1986..2879)
                     /gene="dapA"
                     /locus_tag="PKX82_05025"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOF51646.1"
                     /translation="MNSPQGPIRGSIVALVTPMLDDGSVDYPALRKLIDWHIAEGTDC
                     IGVVGTTGVSPTVSVEEHCEIIRVSVEQAAGRAPIMAGCGANSTKEAIELARFAKKVG
                     ADCQLQVVPYYNKPTQEGQYQHFKAIAEAVDLPVVLYNVPGRSVADMQHDTVLRLAQV
                     PGIVGIKEATGNIERAQWLIKEAPKDFAIYSGDDPTAVALMLCGGHGNVSVTANVAPR
                     LMHELCMAAMAGDVKRAMEIQFKLMPLHKTLFIESNPIPVKWAVARMGLSKGALRLPL
                     TELSEASQKTLEGVMHSTGLI"
     gene            complement(2923..3498)
                     /locus_tag="PKX82_05030"
     CDS             complement(2923..3498)
                     /locus_tag="PKX82_05030"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF51647.1"
                     /translation="MTTALPTHGARGAPSAWIVRWSHLFQPGGTVLDVACGSGRHLSW
                     FAGRGHPVTGVDRDLSGCRDLGDRATLHCADIENGSWPLESGGEPQVFDAVVVTHYLW
                     RPLLPKLLASLAPGGVLLYETFAAGNETVGRPARPDFLLQHGELLQICHGLDIVAYED
                     GYLPDPARFVQRIAAVRPRQDAVSSPRLYTL"
     gene            complement(3635..4855)
                     /locus_tag="PKX82_05035"
     CDS             complement(3635..4855)
                     /locus_tag="PKX82_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF51648.1"
                     /translation="MPNSTPRLSIPQLLFCGAAIVTLSMGIRHGFGLWLQPITQARDW
                     SRETFAFAIAIQNLVWGFSGIFAGMAADRFGAFRVILVGAVLYALGLIGMAHADTPLL
                     FSLSSGVLIGMAQAGTTYAVIYGVIGRNVSAEKRSWAMGVAAAAGSFGQFLMVPTEGF
                     LIMQFGWQQALVLLGLAAILIMPLAWGLREPGFAGSGPARREQTILQALKEAFKYPSF
                     QLLMAGYFVCGFQVVFIGVHMPSYLKDKGLSPQVASYALALIGLFNVFGTYAAGVLGQ
                     RMQKKNILAFIYFSRAIAISLFLLAPLTPMSVYVFSAVMGLLWLSTVPPTNATVAQIF
                     GVAHLSMLGGFVFFSHQIGSFMGVWLGGFLYDRTGSYDVVWYIAIALGVFAALINLPV
                     KEVAIQRTGAAAAH"
     gene            4992..>6565
                     /locus_tag="PKX82_05040"
     CDS             4992..>6565
                     /locus_tag="PKX82_05040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOF51649.1"
                     /translation="MATKPTPEYSEGSIRVLKGLEPVKQRPGMYTRTDNPLHVIQEVL
                     DNAADEALAGHGKKIKVTLHTDGSVSIEDDGRGIPFGLHPEEKAPVIELVFTRLHAGG
                     KFDKGKGGAYSFSGGLHGVGVSVTNALGKRLEATSYREGSVARIVFEGGDVTEALEVR
                     KAGEGDRKSGTTVRVWPDAKYFESSALPMGELVRLLRSKAVLMPGVSVTLVQEKTKET
                     QNWLYKAGLRDYLMQTLHGDPVIPLFEGEGFADSQSDNFAEGEGAAWCVAFTEDGQPV
                     RESYVNLIPTSAGGTHESGLRDGLFTAVKSFIELHSLLPKGVKLMPEDVFARASYVLS
                     TKVLDPQFQGQIKERLNSRDAVRLVSSFVRPALELWLNQHVEYGKKLAELAIRAAQTR
                     QKAGQKVEKRKSSGVAVLPGKLTDCESRDILYNEVFLVEGDSAGGSAKMGRDKENQAI
                     LPLRGKVLNTWEVDRDRLFANTEIHDISVAIGVDPHGPNDTPDMSGLRYGKVCILSDA
                     DVDGSHIQVLLLTLFFR"
BASE COUNT         1313 a         1940 c         2051 g         1261 t
ORIGIN      
        1 tcgcccagct ggtgcgcgat gcgctttcga ctgcggcgtc gcaggggtgg accgagatgg
       61 tgtggagcga tccaaatttt gaggattggc cgctacggga aagagtggtg gtcgatgcac
      121 tccaggcctg ggcgggccgt ggccgaaaac tggtcatgtt ggcccatagt tttgattcgg
      181 tgatccggtt tcaaccgcgc tttgtgcagt ggcgtaccac ctgggaccat gttgtggagt
      241 gtcgggtgtg caggcaacta gatgccagtg aagtgcctag cgcattgtgg agtccgcact
      301 gggccatgcg cagactcgat ctggcgcgct ccacagggat ggcgggttca gaaccccagc
      361 gacgggtgtt gttgcgggag accctggaag agtgccgacg caacagcgcc ccggggtttc
      421 catcgaccgt gttgggtctt tagttgtcag tgcctagggt ttccccgggt gtcggatgcg
      481 aaaaattgta taattcaagg ctctgacaat aagagatcga atgcttcttg tcggggtttt
      541 ggtggcttgc taccaagaca atttccggaa attgtttcgt taactcactt tagtctttca
      601 aaatgaacaa atctctcgtt ctggcagctg ttatcgctgc tgctgccctg gctgcttgca
      661 gtaaaaaaga agaagctgct gctcctgttg ctcccgctcc cgcagctgtt gctcccgcag
      721 ctgacgcttc tgcacccgca gcagctcccg ccgctgttgc tcccgcagcc gctgcagctt
      781 ccgagccagc caagcagtaa ttcgctgccc aagcaaaaaa gccaccgcaa ggtggctttt
      841 ttttgtcccg gccccgcagg gcagggctta tgcacatcag cggatgtcgt cagcaatcac
      901 tttgacaatg cgctgtgcgt tggcagaact ctctggtcca cctttggcgt ccagaatcag
      961 cacggttgtg gcgtcgccct ggctgcgcac cgcaatctgg tacttggtgg gttcaatggt
     1021 cttggatgat ccactgaata tcttgctgaa gaagccaggc tcagagttct cggaacccgg
     1081 ggccacataa cgcacgaagt aaattccctg cttgcggtcc cggtcttcca cggtgaagcc
     1141 ggtgcggtcc agtgccagtc cgacacggcg ccatgcacgg tcgaagtttt cctcgatgag
     1201 gaccacaggt tgcccgttgg tgctcgatac cgttgctacg gatttggcca cactgttggc
     1261 taccagcgcc ttggactgct ccgggctgac gcccagcttg accatcaggc ggcgcaggaa
     1321 ctcggcttcc aattcggggt cggtggggcg gggttgccat accgtgtcac gcttttgctc
     1381 ggaggtgtag acctcaatta cgccacggtg gctgatgaaa atttcggtct caccactggc
     1441 attacgctcc agacgggtcc ggaacttgtc gcgctcggcc gtcgagtaca gggcatccac
     1501 cagtttgccc agtgccgccc ggatggcatc ctgcggaatc ttggcccggt tttcagccca
     1561 gtcggtttcc atgatgccca ggctggactg gtccatcgcc agcaggaagc cgttttcctg
     1621 ccagaagtcc ttgacgggct cccacagcgt ttccgcaggg cgcttgacca ccagccagcg
     1681 ctggttgcct gcgcgctcaa tacgcacatc tccaagggca acggcagcga ccggcgtctt
     1741 ggacgccgat gcctgtcccg actggtatcc ggaggcagac acgccaccgt ccaccacggt
     1801 gtagcgcgtg tccttggaca gttgtgtcag gtcaggcggc accgtcagcg atggcgcttt
     1861 gcctgtgctg cggtagtcca ccttgtcggt ttcgagtgca gtgcaggctg acaacgccag
     1921 ggcaacagcg gtcagaccca gtttggaaat tgaattcacg cgattcctca atcgaatagg
     1981 ggagctcaaa tcagccccgt agagtgcata acgccttcca gcgttttctg gctagcctcg
     2041 gacagctctg tcagtggcag gcgcagtgca cccttgctca gacccatccg tgccaccgcc
     2101 catttcacgg gaatcgggtt ggattcaatg aacagggtct tgtgcagcgg catcagcttg
     2161 aactggattt ccatcgcacg cttgacgtcg ccggccatgg cggccatgca aagttcgtgc
     2221 atcaggcggg gtgccacgtt ggccgtgacg ctcacgttac catggccgcc acacagcatc
     2281 agggccactg cggtgggatc gtcgccagag taaatggcga agtccttcgg tgcttccttg
     2341 atcagccact gggcgcgttc gatgttgccg gtggcttcct tgatgccgac gattccaggc
     2401 acctgcgcca gacgcagcac ggtgtcgtgc tgcatgtctg caaccgagcg gccgggcacg
     2461 ttgtacagga cgacgggcag atccaccgct tcggcaatcg ccttgaagtg ctggtactgg
     2521 ccttcctggg tcggcttgtt gtagtagggc accacctgca actggcagtc ggcgccgacc
     2581 ttcttggcaa agcgggccag ttcaatggct tccttggtgg agttggcgcc acatccggcc
     2641 atgatcggtg cgcggcctgc ggcctgctcc accgacacgc ggatgatttc gcaatgctct
     2701 tcgacgctga cagtggggga cacgcccgtg gtgccgacca cgccaatgca gtcggtgcct
     2761 tcagcgatgt gccagtcgat cagtttgcgc aacgcagggt agtccacgga gccatcgtca
     2821 agcatgggag ttacgagggc gacgatgctg ccccggatcg gaccttgtgg agagttcatg
     2881 tctggctcaa atcaagcgtt gtaaggtaat caatcatttt acctagagtg tgtaaaggcg
     2941 cggtgaactc acggcgtcct ggcgtggacg cactgccgca atccgttgta caaagcgcgc
     3001 cgggtcgggc agatagccgt cttcataggc cacgatgtcc agtccatggc agatttgcag
     3061 cagttcaccg tgctgcaaca gaaagtcagg gcgtgctggt cgccctacgg tttcgttgcc
     3121 tgctgcaaaa gtctcgtaca gcagcacgcc accgggagcc aggctggcca gaagcttggg
     3181 cagcaagggg cgccagaggt agtgggtgac gaccactgcg tcaaatacct gcggctcgcc
     3241 accggactcc agcggccagg agccattctc aatgtcggcg caatgcagcg tcgcccggtc
     3301 gccgagatcc cggcagccgg aaaggtcacg gtccaccccg gtgaccgggt gcccccgacc
     3361 agcaaaccaa gacagatggc gccccgatcc gcaggccacg tccagcacgg tccccccagg
     3421 ctggaacaga tgactccagc gcacgatcca ggccgatggg gcaccccgtg caccgtgggt
     3481 tggcagcgct gtggtcatgc gctgatcaga agcaggacca cagctggttg gccatctgca
     3541 ccagaaagtc cggctgggtg tacatcgcaa agactgccat cagcacggcc agggcaatgg
     3601 tggccaagag gatattgcgt cgtgtcggca tggctcaatg tgcggctgca gcgccggtgc
     3661 gctggatggc cacttctttc accggcaggt tgatgagtgc ggcaaacacg cccagcgcaa
     3721 tcgcgatgta ccagaccacg tcgtaactgc cggtgcggtc gtacaggaag ccgccgagcc
     3781 agacgcccat gaacgagccg atctggtggc tgaagaagac gaagccgccg agcatggaca
     3841 gatgcgccac accgaagatc tgcgccaccg tggcattggt cggtggtacg gtcgacagcc
     3901 acagcagccc catcacggcg gagaatacgt acacgctcat gggcgtgagg ggcgccagca
     3961 ggaacaggct gatggcgata gcgcgcgaaa agtagatgaa ggccagaatg tttttcttct
     4021 gcatgcgttg ccccaggacg ccagcggcgt aggtgccaaa cacattgaac aggccaatca
     4081 gcgccagcgc gtagctggcc acctggggcg acaagccctt gtccttgagg tagctgggca
     4141 tgtggacacc aatgaagacc acctgaaagc cacacacaaa gtagcccgcc atcagcaact
     4201 ggaagctggg gtacttgaag gcctctttca atgcctgcag gatggtctgt tcgcggcgtg
     4261 ccgggccgga gccagcaaaa ccgggttcac gcagtcccca ggccagtggc atgatcaaaa
     4321 tggccgccag acccagcagc accagtgcct gctgccagcc aaactgcatg atcagaaaac
     4381 cttcggtcgg caccatcagg aactggccga acgagcctgc cgcggcggca acgcccatgg
     4441 cccaggaccg cttctcagcg ctcacattgc gcccgatcac gccgtagatc acggcatagg
     4501 tggtacctgc ctgcgccatg ccaatgagca cgccgctgga cagggagaat agcaacggcg
     4561 tgtccgcatg ggccatccca atcaggccca gtgcatacag gacggcaccg accagaatca
     4621 cccggaaggc gccaaagcgg tcggctgcca tgcccgcgaa aataccggaa aagccccaca
     4681 ccaggttctg gatggcgatg gcgaatgcaa aggtttcgcg gctccagtcc cgcgcttgcg
     4741 taataggctg caaccacagc ccgaagccgt ggcggatgcc catggacagg gtcacaatgg
     4801 cggcgccgca gaacagtaac tggggtatgg agaggcgtgg cgtggaattc ggcatggcct
     4861 caaatgtagc cagtttgtac agccgggatg gtcgcccggc aggaatgccc ccggttgcgg
     4921 gttgccagga agatccttga atatgtatgg acatacagtt atttcaagcc agccgtttac
     4981 aatggcggtc tatggcaacc aaacccactc ccgaatattc tgaaggttcg attcgcgttc
     5041 tcaagggcct ggagcccgtc aagcagcgcc cgggcatgta cacccgcacc gacaacccgc
     5101 tgcacgtgat tcaggaagtg ctggacaacg ccgctgatga ggcgctggcc ggccatggca
     5161 aaaaaatcaa ggtgaccttg cacaccgacg gttcggtgtc gatcgaggac gatggccggg
     5221 gcattccatt cggactgcat ccggaagaaa aggcgcctgt gattgaactg gtatttaccc
     5281 gtctgcatgc cggtggcaag ttcgacaagg gcaagggcgg cgcctacagc ttctccggtg
     5341 gtctgcacgg cgtgggcgtg agcgtcacca acgcactggg caaacggctg gaagccacca
     5401 gttaccgtga aggttcggtg gcgcgcattg tgtttgaggg cggtgacgtc accgaagcgc
     5461 tggaggtccg caaggcaggc gagggtgacc gcaaatccgg caccacggtg cgcgtctggc
     5521 cggacgccaa atactttgaa tcttccgccc tgcccatggg tgagctggtc cgcctgctgc
     5581 gcagcaaggc ggtgctcatg cctggcgtca gcgtgacgct ggtccaggaa aaaaccaagg
     5641 aaactcagaa ctggctctac aaggcgggtc tgcgcgatta cctgatgcag acgctccatg
     5701 gcgaccctgt gattccgctg tttgaaggtg aaggttttgc ggacagccag agcgacaact
     5761 ttgccgaagg cgaaggtgct gcgtggtgtg tggcgtttac cgaagacggc cagccggtgc
     5821 gcgagagcta cgtcaatctg attcccacca gtgccggtgg cacgcacgag agcggcctgc
     5881 gcgatggcct gttcacggca gtcaagagct tcattgaact gcactcgctg ctgcccaagg
     5941 gcgtgaagtt gatgccggaa gacgtgtttg cgcgtgccag ctatgtgctg tccaccaagg
     6001 tgctggaccc gcagttccag ggccagatca aggagcgcct gaattcccgc gacgcggtgc
     6061 ggctggtgtc gagctttgta cgtccggcgc tggagttgtg gctcaaccag cacgtcgaat
     6121 acggcaagaa gctggccgag ctggcgatca gagccgcgca gacgcgccag aaggccggcc
     6181 agaaggtgga gaagcgcaag agctcaggcg tggccgtgct gccgggcaag ctcaccgatt
     6241 gcgaaagccg cgacatcctc tacaacgaag tgtttctggt cgagggcgac tccgctggcg
     6301 gtagcgccaa gatgggccgc gacaaggaaa accaggccat cctgccgttg cgtggcaagg
     6361 tgctcaacac ctgggaagtg gaccgcgacc ggctgtttgc caacaccgaa atccacgaca
     6421 tttccgtcgc tatcggtgtg gacccccacg gccccaacga cacgcccgat atgagcggcc
     6481 tgcgctatgg caaggtctgc atcctgtccg atgcggatgt ggatggctcg cacatccagg
     6541 tgctgctgct gacgctgttt ttccg
//
LOCUS       DAOSJM010000102         6552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10702_length_6552_cov_3.474065, whole genome shotgun sequence.
ACCESSION   DAOSJM010000102 DAOSJM010000000
VERSION     DAOSJM010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6552
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10702_length_6552_cov_3.474065"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PKX82_05045"
     CDS             <1..1009
                     /locus_tag="PKX82_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOF51650.1"
                     /translation="ALQWMAPGALAGNWLGVAVHSWTDSSSAPVLAALPPADVWVEGF
                     GCEIAPEFIAARAYSTGATGQNNIKPPVWINLEYLSAESYVERAHGLPSPVLAGPARG
                     WTKHFYYPGFNQRTGGLLREPDLLERRHQFDRDAWLRQIGVTPQTSERLVSLFCYEPP
                     ALDLLLTQLVQWPDRTRLLVTPGRATAAVKAALEHKNSLQPAWNMREALSISYLPTLS
                     QRDFDHLLWACDLNCIRGEDSLVRALWAGKPLLWQIYPQSDAAHHEKLDAFLDMLGAS
                     ASLRRWHAVWNGLEVPTAINALAHADLENWSQITHMARSRLLELGDLTSGLVDFVLKK
                     R"
     gene            1173..1727
                     /gene="efp"
                     /locus_tag="PKX82_05050"
     CDS             1173..1727
                     /gene="efp"
                     /locus_tag="PKX82_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOF51651.1"
                     /translation="MKIAQEIRAGNVIMHGKDPMVVLKTEYSRGGRNSATVRMKLKSL
                     IANFGTEVVFKADDKLDQVILDKKDCTYSYFADPLYVFMDAEYNQYEVEAENMGDTLN
                     YLEDGMAVEVVFYDGKAISVELPTSVVREITWTEPAVKGDTSGKVLKPAKIATGFEIG
                     VPIFVAQGDKVEIDTRTGEYRKRV"
     gene            1805..3406
                     /locus_tag="PKX82_05055"
     CDS             1805..3406
                     /locus_tag="PKX82_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase family protein"
                     /protein_id="HOF51652.1"
                     /translation="MTDVSPLQTPPQDPSADDHSRLAKLTLRRVLIDTYHENVAYMHR
                     DCEIYRAEGFKALSKIEIRTNGHSILATLNVVDDHNIVACGELGLSKAAFAQLDVEEG
                     HSATVAQAEPPASIGALHRKIDGERLRAEDFHSIVQDIANHRYSKIELTAFVVACDRD
                     ELDREEVFFLTDAMIAAGRHLNWHETLVVDKHCIGGIPGNRTSMLIVPIVAAHGMLCP
                     KTSSRAITSPAGTADTMEVLANVELPMGQLQDIVRTHRGCLAWGGTANLSPADDVLIS
                     VERPLSLDSPGQMVASILSKKISAGSSHLVLDIPIGPTAKVRSMPEAQRLRRLFEYVA
                     RRMKLSLDVVITDGRQPVGNGIGPVLEARDVMQVLENDPRAPIDLRQKALRLAGRLIE
                     CDPDVRGGDGFAIARDILDSGRALTKMNDIILAQGSKSFDHHQPDLGLLTLEICAQTA
                     GVVTGIDNLQMARIAGLAGAPKVPSAGVDLLRKLGDEVQVGQPLYRVHASYAADLEFA
                     RQACARSTGYTIGSAAEVPHVFVEF"
     gene            3406..4287
                     /locus_tag="PKX82_05060"
     CDS             3406..4287
                     /locus_tag="PKX82_05060"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOF51653.1"
                     /translation="MPSETLLLHFAEDEASATRIAQAANMAMARIERHRFPDGELKLR
                     LPPQLPPHVVLLRTLDHPNEKLVELLLVARTARQLGATHLTLVAPYLAYMRQDIAFNP
                     GEVVSQRVVGAFLAGLFDAVITVDPHLHRVATLEEAVPVPRAVVLSGAPLLADWIATR
                     VEKPLLVGPDEESAQWIAQAAAQHQFDFAVCRKVRHGDRHVEVALPTVDVKGRNVVLL
                     DDVASSGHTLAEATRLLLAAGASRVDVAVTHALFADEALQLVKTAGVSEVWSTDCIAH
                     STNTVSMASAIAAALTH"
     gene            4295..5167
                     /locus_tag="PKX82_05065"
     CDS             4295..5167
                     /locus_tag="PKX82_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOF51654.1"
                     /translation="MTATQHHTLNERTLADAWADLQAHADAGLALHADAYRLAFSDPE
                     FLLRRETRGIRFQLEMLKPDLDQTQAGVENTVVVFGSARFPSPESAQKSLEEAQQGND
                     AKALAVAQRKVRNAAYYDQARLFARLVAAYSNARPAAERLFIATGGGPGIMEAANRGA
                     HEMGAPNVGLNIALPHEQSHNPYITPELCFKFHYFALRKMHFMMRAKALVAFPGGFGT
                     LDELFEVITLVQTGKAKPVPIILFGSNYWKRLLNLDVLIEEGAISPDDVSLFQYTDDP
                     QEAWDAIKAFYQLG"
     gene            complement(5176..6300)
                     /locus_tag="PKX82_05070"
     CDS             complement(5176..6300)
                     /locus_tag="PKX82_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF51655.1"
                     /translation="MFRLIGGHICLHASMAGLRMAAPLFALSLGYSALSVGLLLALFS
                     LSQVFLAIPAGRFADRHGLRRPIALGVAVACSGAALSVLFPRFEVLCFSALCLGGATG
                     ASAIALQRHVGRAASSTLELKQVFGWLSIGPAVANFVGPVAAGLLIDHAGYRWAFLLS
                     AVLPLCTWLFVRHTKELPSIPVPQAGATAQAWALLGEPRMRRLLLVNWLLSSCWDVHT
                     FVVPLLGHERGFSASVIGTILGSFAVAAACVRVLMPVVAAHLREWVVVSGAMVATALL
                     FGIYPLMVSPWTMGLCSVLLGLALGMVQPMIMSTLHQITPHHLHGQAIALRLMSINLS
                     SVLMPVLFGTAGAVVGVSVVFWTVGSIVGLGARAAWHLRP"
     gene            complement(6348..>6552)
                     /locus_tag="PKX82_05075"
     CDS             complement(6348..>6552)
                     /locus_tag="PKX82_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOF51656.1"
                     /translation="FGKFGDVLAKVKAQTQTVLNTLDSAETRSRAMGRALKKVEALPE
                     TQAQALLPGDMEAESGSDGDALA"
BASE COUNT         1336 a         2170 c         1930 g         1116 t
ORIGIN      
        1 ggccttgcag tggatggcac cgggcgcgct ggcgggaaac tggctggggg tggccgtgca
       61 ttcctggact gacagctcca gcgccccagt actggccgca ctgcctcctg ccgacgtatg
      121 ggtggagggc tttggttgcg agattgctcc tgaattcata gctgcccgcg catattctac
      181 gggggctaca ggccagaata atataaaacc tcccgtgtgg atcaatctgg agtacctcag
      241 cgccgaatcg tatgtggagc gtgcgcacgg cctgccctcc cccgtgctgg cgggcccagc
      301 ccgtggctgg accaagcatt tctattaccc gggcttcaac cagcgcaccg gtgggctgct
      361 gcgcgagccc gatctgctgg agcgccggca ccagttcgac cgggatgcct ggctgcgcca
      421 gatcggagtg accccgcaga cgagcgagcg actggtaagc ctgttttgct acgaaccgcc
      481 cgcgctggat ctgctgctca cccagctggt gcagtggcct gaccgcacgc gcctgctggt
      541 cacgcccgga cgtgccacgg cggctgtaaa agccgctttg gagcataaaa acagcctcca
      601 gcccgcatgg aacatgcgcg aagcgctatc aatttcatac ttacccacat tatcccagcg
      661 cgactttgac catttgctgt gggcctgcga cctcaattgc atacgcggcg aagattcgct
      721 ggtgcgtgcg ctgtgggctg gcaaaccgct gctgtggcag atctacccgc agagtgacgc
      781 cgcacaccac gaaaagctcg atgcattcct ggacatgctg ggcgccagtg ccagtttgcg
      841 ccgctggcac gcggtgtgga acggcctgga agtacccact gcaatcaacg ctctggccca
      901 cgccgatctg gaaaactggt cgcaaatcac gcacatggca cggtcgcgcc tgctggaatt
      961 gggtgatttg accagcggcc tggtcgattt cgtgctgaaa aaacgttaaa attcaaggct
     1021 ttgctgagat agccggccaa tgtgggctgg tcattccagc cccgccgcca agaatatgga
     1081 cggcgtcttc gccgccagga atatggacgg cgtcttcgcc gctatgtgcg gtctacttac
     1141 gattcgtggc cctacaaaac cactcgacac ctatgaaaat cgctcaagaa attcgcgccg
     1201 gcaacgtcat catgcacggc aaggacccca tggtcgtcct gaagaccgaa tacagccgcg
     1261 gcggccgcaa ctccgccacc gtgcgcatga agctcaaaag cctgatcgcc aacttcggta
     1321 ccgaagtcgt gttcaaggcc gacgacaagc tggaccaagt gattttggat aagaaggatt
     1381 gcacctattc ctattttgcc gacccgctgt acgtgttcat ggacgccgag tacaaccagt
     1441 acgaagtcga agccgaaaac atgggcgaca cgctgaacta cctcgaagac ggcatggccg
     1501 ttgaagtggt gttctacgac ggcaaagcca tctccgtgga actgcccacc agtgtggtgc
     1561 gcgaaatcac ctggaccgaa cccgccgtca agggcgatac ctccggcaag gtgctcaagc
     1621 ctgccaagat cgctaccgga ttcgagatcg gcgtgccaat cttcgtggcc cagggcgaca
     1681 aggttgaaat cgacacccgc accggcgaat accgcaagcg cgtgtaatct ggctcccacg
     1741 cttgatgcca aaggctcctt cgggagcctt tttcatgccc gtcgattcgg tgtaggctac
     1801 agggatgact gacgtgtccc cactgcaaac tccgccgcaa gatccatcgg ccgacgacca
     1861 ctcgcgtctg gccaaactga cgctgcgccg cgtgctcatt gatacctacc atgagaacgt
     1921 ggcctacatg caccgcgact gcgagatcta ccgggctgaa gggttcaagg ccctctcgaa
     1981 gatcgagatc cgcaccaacg gccacagcat cctggcgacc ctcaatgtcg tggacgacca
     2041 caacatcgtg gcctgcggcg aactgggcct gtccaaagcc gcttttgcgc aactggacgt
     2101 agaagaagga cactccgcca ccgtggcgca ggcggagccg cctgcatcca tcggtgccct
     2161 gcaccgcaag atcgacggcg agcgcctgcg ggccgaggac ttccactcca tcgtgcagga
     2221 cattgccaac caccggtatt ccaagattga actgaccgcc tttgtggtgg cctgcgaccg
     2281 cgacgaactc gaccgcgaag aagtgttctt tctgaccgac gccatgattg ccgcgggccg
     2341 ccacctcaac tggcatgaaa ctctggtggt ggacaaacac tgcatcggcg gtatcccggg
     2401 caaccgcacc tcgatgctga tcgtgcccat cgtggctgcc cacggcatgc tgtgccccaa
     2461 gacctcgtcg cgtgccatca cctcccccgc cggcacggcc gacaccatgg aagtgctggc
     2521 caatgtggaa ctgcccatgg gccagttgca ggacatcgtg cgcacccacc gcggctgcct
     2581 ggcgtggggc ggaaccgcca acctgtcgcc tgcagacgat gtgctgatct cggtggagcg
     2641 cccgctctcg ctggactctc cgggccagat ggttgcctcc atcctgtcga agaaaatttc
     2701 tgccggctcc agccatctgg tgctggacat ccccatcggc cccaccgcca aggtgcggtc
     2761 catgcccgaa gcccagcgcc tgcgtcggct gtttgaatat gtggcgcgac gcatgaagct
     2821 gtcgctggac gtggtgatta ccgacgggcg ccagccggtg ggcaacggca tcggcccggt
     2881 gctggaggcc cgcgatgtga tgcaggtgct ggaaaacgat ccccgtgccc ccatcgacct
     2941 gcggcagaag gcgctgcgcc tggcgggtcg actcatcgag tgcgatccgg atgtgcgtgg
     3001 cggcgatgga ttcgccatcg cccgggacat cctggactcg ggccgtgcac tgaccaagat
     3061 gaacgacatc atcctggccc agggcagcaa gtccttcgac caccaccagc cggatctggg
     3121 tctgctgacc ctggaaatct gcgcgcagac ggcgggtgta gtgaccggta tcgacaacct
     3181 gcagatggcg cgtattgccg gactggccgg cgcccccaaa gtgcccagtg cgggcgtgga
     3241 cttgctgcgc aagctcggcg acgaggtgca ggtaggccaa ccgctgtacc gggtgcacgc
     3301 atcctatgca gcggacctgg agttcgcgcg ccaggcctgc gcccgctcga ccggctacac
     3361 catcggcagc gcagccgaag tgccccatgt ttttgtggag ttttaatgcc ttcagaaacc
     3421 ctgctgctgc actttgcaga agacgaggcc agtgccacgc gcatcgccca ggcggccaac
     3481 atggcgatgg cccggatcga acgccaccgt tttcctgacg gtgaactcaa gctgcgtctg
     3541 ccgccccagt tgccacccca tgtggttttg ctgcgcacgc tggaccaccc caatgaaaaa
     3601 ctggtggaac tgctgctggt ggcgcggact gcgcgccagc tcggtgccac ccacctgacc
     3661 ctggtcgcac cctacctggc ctatatgcgc caggacattg cattcaaccc gggggaagtc
     3721 gtcagccagc gcgtggtggg cgcatttctg gcaggcctgt tcgatgccgt cattaccgtg
     3781 gacccgcatc tgcaccgtgt ggcgacgctg gaggaagccg tacccgtccc gcgcgccgtg
     3841 gtcctcagcg gcgccccgct gctggcggac tggattgcca cgcgggtgga aaaacccctg
     3901 ctggtgggcc ccgatgaaga gtctgcccaa tggatcgccc aggcggccgc acaacaccag
     3961 tttgactttg cggtatgccg caaagtgcgc cacggcgacc gccatgtcga agtcgcgttg
     4021 ccgaccgtcg atgtcaaagg ccgcaatgtg gtcctgctcg atgatgtggc cagtagcggc
     4081 cataccctgg ccgaagccac gcgcctgctg ctggccgccg gggccagccg tgtggacgtg
     4141 gccgtgaccc acgccctgtt tgccgacgag gccctgcaac tggtcaaaac ggccggggtc
     4201 agtgaagtct ggagcaccga ctgcattgcc cattccacca atactgtcag catggcgagc
     4261 gccattgccg cagccctcac gcactgaact gaatatgacc gccacccaac accacaccct
     4321 caacgaacgt actctggccg atgcctgggc cgatctgcaa gcccatgccg atgcaggcct
     4381 ggcgctacac gccgatgctt accggctggc gttttccgac cccgagttcc tgctgcgccg
     4441 cgagacccgc ggcatccgct tccagctgga aatgctcaag cccgacctgg accaaaccca
     4501 ggctggtgtc gagaacaccg tcgtcgtatt cggtagcgcc cgttttccat ccccggagag
     4561 cgcacaaaag agcctcgaag aagcgcagca aggcaatgac gccaaggcgc ttgcagtagc
     4621 ccaacgtaag gtgcgcaacg ccgcctacta cgaccaggcc cgcctcttcg cccgcctggt
     4681 cgcggcctac agcaacgcgc gtccagcggc ggagcgcctg ttcattgcga cgggcggtgg
     4741 ccccggcatc atggaagctg ccaaccgtgg tgcgcatgaa atgggggccc ccaacgtcgg
     4801 cctgaacatt gccttgccgc acgagcagag ccacaacccc tacatcacgc cggaactgtg
     4861 tttcaagttc cactattttg cgctgcgcaa gatgcacttc atgatgcgcg ccaaagcgct
     4921 ggtggcgttt cccggcggct tcggcacgct ggacgaactt tttgaagtga tcacgctggt
     4981 gcaaaccggc aaggccaagc cagtgcccat catcctgttt ggcagcaatt actggaagcg
     5041 cctgctcaac ctcgacgtgc tgatcgagga aggcgccatt tcaccggacg acgtgagcct
     5101 gttccagtac accgacgatc cgcaggaagc gtgggacgcc atcaaggcgt tctaccagct
     5161 cggctgacca ccccatcagg ggcgcaaatg ccaggccgcc cgggcaccca gtccaacgat
     5221 ggaacccacc gtccagaaca ccacggacac gcccaccacg gcacctgcag ttccaaacag
     5281 cacgggcatg agtacgctgg agaggttgat gctcattagc cgcaatgcaa tggcctgacc
     5341 atgcaggtgg tgcggcgtga tctggtgcag ggtgctcatg atcatgggtt gcaccatacc
     5401 cagggccagc cccagcagca ccgagcacag gcccatggtc cagggcgaca ccatcaacgg
     5461 gtaaatgcca aacaacagtg ccgtggccac catggcacca ctgaccacca cccactcccg
     5521 caaatgggca gccaccaccg gcatcaatac ccgcacacaa gccgctgcca cggcaaatga
     5581 acccagaatg gttccgatga ccgaggcact gaaaccccgc tcatggccca gcagggggac
     5641 cacaaaagtg tgcacatccc agcacgaaga cagcagccag ttcaccaaca gcaggcggcg
     5701 catgcgcggc tcacccagca gtgcccaggc ttgggcggtt gcacctgcct gtggcacggg
     5761 aatggacggt aactctttcg tatggcgcac aaacagccag gtgcacagcg gcaggacggc
     5821 agacagcaaa aaagcccagc gataacccgc atggtcaatc agcagccccg ctgccactgg
     5881 ccccacaaaa ttggccacgg cgggaccaat ggacagccag ccaaacactt gcttgagttc
     5941 cagcgtactg ctggctgcgc ggcccacgtg ccgctgcaag gcaatggccg atgccccggt
     6001 tgcgccgccc agacataagg cactgaaaca caacacttca aaacggggaa acagcaccga
     6061 caatgccgcg ccactacaag ccaccgccac gcccagggca atgggtcgac gcaatccgtg
     6121 ccggtcggca aaacggcccg ccggaatggc cagaaacacc tgggacaagg aaaacagtgc
     6181 cagcagcaag cctaccgaca aggcgctata gcccaggctg agtgcgaaga ggggggctgc
     6241 catgcgcagg cccgccatgc tggcgtgcag gcagatatgg cccccaatca agcgaaacag
     6301 taccccgggc ccgtggctgc gatgcccttg gggcgcatcc tgcgcagtca cgccaatgcg
     6361 tctccatcgc tgccggattc ggcttccata tcacccggca gcagggcctg cgcctgcgtt
     6421 tccggcaagg cttctacctt cttgagcgcg cgacccatgg cgcggcttcg ggtctcggca
     6481 ctgtccagcg tattgagcac cgtctgggtc tgtgccttga ccttggccag cacatccccg
     6541 aacttgccaa ac
//
LOCUS       DAOSJM010000103         6550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10704_length_6550_cov_4.371517, whole genome shotgun sequence.
ACCESSION   DAOSJM010000103 DAOSJM010000000
VERSION     DAOSJM010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10704_length_6550_cov_4.371517"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..661
                     /locus_tag="PKX82_05080"
     CDS             89..661
                     /locus_tag="PKX82_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796207.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HOF51657.1"
                     /translation="MVRYPVPDLNSLPEDIRAKILEVQEKSGFIPNVFLAFGRRPAEW
                     RAFFAYHDALMLREESNLTKGDREMIVTTTSAANHCLYCVVAHGAILRIYEKKPLVAD
                     QVAVNYRKADITPRQRAMLDFAMKVCKNSDQVEDADFKTLHAHGFNDEDIWDIAAITA
                     FFGLSNRMANVTGMLPNPEFYTLGRTPRQK"
     gene            675..2510
                     /locus_tag="PKX82_05085"
     CDS             675..2510
                     /locus_tag="PKX82_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOF51658.1"
                     /translation="MRACRNFGWAALAAVCLWLAGCAHPSAVPGSDASAQPEGASGQV
                     AKPGWATRTYAVAAANPLGTEAGQQILQAGGSAVDAAIAVQMVLTLVEPQSSGIGGGA
                     FLLHFDGHQVQAYDGRETAPAGATPGLFLDASGRPMPFADAVVGGRSVGVPGTVRMLA
                     LAHQRHGKLPWADLFQPAVALATQGFRVSPRMATLLAADPHLAKDPVAAAYFFDTDGK
                     PWPAGHLLRNPELAAVLQRLAREGPDALMRGDIAQAMVDKVHQHPTNPGTLSLQDLQA
                     YTPKVREPLCFDYAAAAPARAYRICGMPPPSSGALAVGQILGLLQSTDAQRLPLAQGL
                     PTADWLHLYTEAARLAFADRAQYVADPDFVQAPAGSWGSLLEPAYLAARAKRMDGGPL
                     GKRMKVAPAGTPGKVPVAYAPMPDQPEYGTSHISIVDRWGQALAMTTTIEDGWGARLM
                     VNRGLGLRGGFLLNNELTDFSFTPADADGRPVANRVEPGKRPRSSMAPTLVLDQTSGQ
                     LVMSTGSPGGAFIIHFTAKALYGMLNWGLDAQQAINLPNFGTLGGPVLLEENRFPPDT
                     NQALRERDHPVTELPLPSGVQAIQRTPAGYFGGADPRREGVVLGD"
     gene            complement(2583..3458)
                     /locus_tag="PKX82_05090"
     CDS             complement(2583..3458)
                     /locus_tag="PKX82_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOF51659.1"
                     /translation="MTYTTLTTQIDDGILTLTLNRPEQMNAFTVEMAHELVDVYRSAS
                     ANDAVRAIVVTGAGKAFCAGMDLSRPGNVFGLNEDLQPTLQDMQERLDDPEVLQGVRD
                     TGGLISLAVYDCTKPVIGAINGAAVGIGATMTLPMDVRLASDKARIGFVFGRIGIVPE
                     ACSSWFLPRIVGISQALEWAYCADILTPEEALQGRLIKAIVPHDQLLAEAHKLAHRMT
                     DGRSPVGIALTRQMMYRNAAQPHPLEAHKVDSLAMFYTSVNDGKEGVRAFLEKRPAQF
                     TSRTSTDMPGFYPWW"
     gene            3519..5483
                     /gene="dnaG"
                     /locus_tag="PKX82_05095"
     CDS             3519..5483
                     /gene="dnaG"
                     /locus_tag="PKX82_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOF51660.1"
                     /translation="MAIPQSFIQELLARTDVVEIVGRYVQLKKGGANFMGLCPFHGEK
                     SPSFSVSPTKQFYHCFGCGKNGNAISFLMDHVGMTFIEAVKDLAQQYGMVVPEEEGSP
                     QDRARAQEQREKQHTLTSVLEKASDAYRRHLKDSPRAIAYFKGRGLSGEVAKQFGLGY
                     APEGWRSLASVFPQYDDPLLVESGLVILHEAENGEDEKRYDRFRDRVMFPIRNIKGEC
                     IGFGGRVLGDDKPKYLNSPETPVFSKGRELYGLFEARNALREQGYVLVTEGYMDVVAL
                     AQLGFPNAVATLGTACTADHVHKLFRFTDAVVFSFDGDSAGRRAARKALDAALPYATD
                     VRSIKFLFLPAEHDPDSFIRANGKEAFADYVKQATPLSRFLIEAAREDCDLQTAEGRA
                     RMASNAKPLWSLLPEGTLKLQLLGEIAAQVQLPSQELGALWSATSNTPHTRAPSSARQ
                     TSDWQPDDHDERSPTATPWGHPLDGESTNTSKSKGKWEGKGGDWKGKGKWDRDRPVRP
                     PLRGPLASRADHATRILLGHSALWEALTHEDHQLLCELPAPHGPLMAWLEAQLHEHGP
                     LPWGALKEALVDHADAAHALRLMADTPMPPETASEFTHELRDLLNRMLLEQIEQQKNQ
                     ASAAAASDPAAFQRYRELNERWQQLKHSIS"
     gene            5778..>6550
                     /locus_tag="PKX82_05100"
     CDS             5778..>6550
                     /locus_tag="PKX82_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HOF51661.1"
                     /translation="MPAQKSAKPVQSAAKPASKSASKTEKKAPAVPASKAPVAKSKAT
                     LLKEAADALLAADAPAKKKPGRPAKAATAEASAPPKTGAKRGRKPKAAAESSGDDMDM
                     SDIEADLAGEPVTTPGEKVKPLRMKISKAKERALMKEFGLDETVLSEEDMAKRRLRLK
                     ALIKLGKTRGYLTHGEISDHLPDKLVDAETLEVVISMLNDMGVAVYEQTPDAETLLLN
                     NNGPTAATEEEAEEEAEAALSTVDSEFGRTTDPVRMYMR"
BASE COUNT         1282 a         2201 c         1992 g         1075 t
ORIGIN      
        1 tcgtatgcag tgttcatcgt tctgttcctc agccggatgt caaagagcag aaggataatg
       61 gtttcaaaga caaaaggaga acattgccat ggtccgctac cccgtccccg atctcaacag
      121 cctgcccgaa gacatccgcg ccaaaatttt ggaagtgcag gaaaagtccg gtttcattcc
      181 caacgtgttc ctggcattcg gccgccgccc tgccgagtgg cgtgcgttct ttgcctacca
      241 cgatgcgctg atgctgcgcg aggaatccaa cctgaccaag ggcgaccgcg agatgatcgt
      301 caccaccacc agcgcagcca atcactgcct gtactgcgtg gtagcccacg gcgccatcct
      361 gcgcatctac gagaagaaac cactggtggc ggaccaggtg gccgtgaact accgcaaggc
      421 cgacatcacc ccgcgccagc gcgccatgct cgacttcgcc atgaaggtct gcaagaacag
      481 cgaccaggtc gaagacgccg acttcaaaac gctgcatgcc catggcttca atgacgaaga
      541 catctgggac atcgcggcca tcaccgcgtt cttcggcctg tccaaccgca tggccaatgt
      601 caccggcatg ctgcccaacc ccgagttcta cacgctcggc cgcacgccgc gccagaaata
      661 accaggccga atccatgcgc gcctgccgaa actttggctg ggccgccctg gcggcggttt
      721 gcctgtggct ggccgggtgt gcacacccat cggcggttcc gggttctgac gcatctgcgc
      781 aacccgaggg cgccagtggc caggttgcca agcccggctg ggccacccgc acctacgccg
      841 tggccgcagc caacccgctg gggaccgagg ccggccagca gatcctgcag gcgggtggca
      901 gcgcggtgga cgccgcgatt gcagtgcaaa tggtgctcac cctggtcgag ccccaatcca
      961 gcggcattgg cggcggcgcc ttcctgctgc actttgacgg gcaccaggtg caggcctatg
     1021 acgggcgcga aactgcgccg gccggtgcca caccaggact gtttctggat gccagtgggc
     1081 ggccgatgcc gttcgccgat gcggtggtcg gtggccgttc ggtcggcgtg ccaggcacgg
     1141 tgcgcatgct ggcgctggcc caccagcggc acggcaagct gccctgggcg gacctgttcc
     1201 agcctgccgt ggcactggcc acgcagggct tccgggtgag cccgcgcatg gccaccctgc
     1261 tggccgccga tccgcacctg gccaaagacc cggtggcagc ggcctacttc ttcgacaccg
     1321 atggcaagcc ctggcccgcc gggcacctgc tgcgcaaccc ggagctggcc gccgtgctgc
     1381 agcgcttggc ccgcgaagga ccagacgcac tcatgcgggg ggatatcgcc caggccatgg
     1441 tggacaaggt gcaccagcat cccaccaatc cgggcacgct ctcgctgcaa gacctgcagg
     1501 cctacacgcc caaggtgcgc gaaccgctgt gctttgatta cgcggctgcc gcacccgcca
     1561 gggcgtatcg catctgcggc atgccgccgc ccagctcggg tgcgctggcc gtgggccaga
     1621 ttctgggcct gctgcaaagt accgacgccc agcgattgcc gctggcgcag ggcctgccca
     1681 ccgccgactg gctgcacctc tacaccgaag ctgcgcgcct ggcgtttgcc gaccgggccc
     1741 agtacgtggc cgaccccgac tttgtgcagg cgccagccgg ttcctggggc agcctgctgg
     1801 agccagccta cctggcggcc cgcgccaagc gcatggacgg tggccctttg ggcaagcgca
     1861 tgaaggtggc tcctgcgggc acaccaggca aagtgcccgt ggcctacgcc ccgatgccgg
     1921 accagcccga gtacggcacg tcacatatct ccatcgtcga ccgctggggc caggccctgg
     1981 ccatgaccac caccatcgaa gacggctggg gcgcccggct gatggtcaac cgcggcctcg
     2041 ggctgcgcgg cggcttttta ctcaacaacg aactcaccga cttcagcttc acccctgccg
     2101 atgcggatgg cagaccggtg gccaaccggg tcgagcccgg caagcggccg cgctcgtcga
     2161 tggcgcccac gctggtgttg gaccaaacca gcggccagtt ggtcatgagt acgggcagcc
     2221 cgggcggtgc cttcatcatc catttcacgg ccaaggcgct gtacggcatg ctgaactggg
     2281 ggctggatgc gcagcaggcc atcaacctgc ccaacttcgg cacactcggt ggtccggtgc
     2341 tgctggaaga aaatcgcttt ccaccggaca ccaaccaggc cctgcgcgag cgcgaccacc
     2401 cggttaccga actgcccctg cccagcggcg tgcaggcgat acagcgcacg ccggccggct
     2461 actttggtgg cgcagatcct cgacgcgagg gcgtagtgct gggcgattag aggtcaattt
     2521 ggcctccagc ccccgtatct cttgggagcg cagctactta attgatagca aaccaagcca
     2581 gctcaccacc agggatagaa ccctggcatg tcggtggacg tccggctcgt gaattgcgca
     2641 ggccgtttct ccagaaacgc acgcacgccc tccttgccat cgttcacgct ggtgtagaac
     2701 atggccagcg aatccacttt gtgcgcttcc agcggatgcg gctgcgccgc gttgcggtac
     2761 atcatctggc gcgtcagggc gatgcccacg ggggagcgcc cgtctgtcat gcggtgggcc
     2821 agtttgtgtg cctcggccag cagctggtcg tgcggcacga tggccttgat cagccgcccc
     2881 tgcagggctt cctcgggtgt caggatgtcg gcgcaatagg cccactccag tgcctgcgaa
     2941 atgcctacga tgcgtggcag gaaccagctc gaacaggctt cgggcacgat cccgatgcgg
     3001 ccgaacacaa agccgatgcg agccttgtcg gatgccagcc gcacatccat gggcagggtc
     3061 atggtggcgc caatgccgac cgcggcacca ttgatggcgc cgataaccgg cttggtgcag
     3121 tcgtacaccg ccagtgaaat cagaccgccg gtatcgcgca cgccctgcag cacctccggg
     3181 tcgtccagcc gctcctgcat gtcctgcagg gtcggttgca ggtcttcgtt caatccgaat
     3241 acattgccgg gccggctcag gtccatgccg gcgcagaatg ccttgccggc accggtcacc
     3301 acaatcgcgc gcaccgcgtc atttgcgctg gccgagcggt agacatcgac cagttcgtgc
     3361 gccatctcca cggtaaaggc gttcatctgc tcgggccggt tcagcgtcag ggtcaggatg
     3421 ccgtcgtcaa tctgggtggt cagggtggtg taggtcatgg cgcgggtcac atcagtgaag
     3481 ttgggatgcg ccctagttta ggttcctaca atcaccccat ggccatcccc caatcgttca
     3541 tccaggaact gctcgcccgc accgatgtgg tcgagatcgt gggacgctat gtgcagctca
     3601 agaagggtgg cgccaatttc atggggctat gcccgttcca cggggaaaag tcgccctcgt
     3661 tctcggtcag cccgaccaag cagttctacc actgctttgg ctgcggcaaa aacggcaacg
     3721 ccatcagctt cctgatggac cacgtgggca tgaccttcat tgaagccgtc aaggacctgg
     3781 cccagcaata tggcatggtg gtgcccgagg aagaaggctc cccccaggat cgcgcccgtg
     3841 cgcaggaaca acgcgaaaag cagcacacgc tgaccagcgt gctggagaag gcatcggacg
     3901 cctaccgccg gcacctcaag gattcgccac gcgccattgc ctatttcaag ggccgcggcc
     3961 tgtcgggcga agtagccaaa cagtttggcc tgggctatgc cccggaaggg tggcgcagcc
     4021 tggccagcgt gttcccgcag tacgacgacc cactgctggt ggaaagtggc ctggtgatcc
     4081 tgcacgaagc ggagaacggc gaagacgaaa agcgctacga ccggtttcgc gaccgggtca
     4141 tgtttcccat ccgcaatatc aagggcgagt gcatcggctt tggtggccgg gtgctgggcg
     4201 acgacaagcc caaatacctg aactcgccgg aaacgcccgt gttcagcaag ggccgcgaac
     4261 tctatggcct gtttgaagcc cgcaacgcat tgcgcgaaca gggctatgtg ctggtcaccg
     4321 aaggctatat ggacgtggtg gccctggcgc aactgggctt tcccaacgcg gtggccacgc
     4381 tggggaccgc ctgcaccgcc gaccatgtgc acaagctgtt ccgttttacc gacgccgtgg
     4441 tgttcagctt cgacggcgac tccgccggac gccgcgccgc ccgcaaggcg ctggacgccg
     4501 ccctgcccta cgccaccgat gtacgcagca tcaagttttt gtttttgccc gccgaacacg
     4561 accccgacag ctttatccgg gcgaacggca aagaggcttt tgctgactat gtgaagcagg
     4621 ccacaccgct gagccgtttt ctgatcgagg cagcgcgcga agactgcgat ttgcagacgg
     4681 ccgaaggccg cgcgcgcatg gccagcaatg ccaagccgct gtggagcttg ctgccagaag
     4741 gaaccctcaa actccagttg ctgggcgaaa tcgccgcgca ggtgcagttg ccaagccagg
     4801 aactgggtgc cttgtggtcg gcgaccagta acacgcccca taccagagcc ccgtcctcgg
     4861 cgcggcagac cagcgattgg caaccggacg accacgacga acgcagcccc accgccaccc
     4921 catggggcca cccactggat ggtgaaagca ccaatacctc gaaaagcaag ggcaaatggg
     4981 aaggcaaagg gggggactgg aaaggcaaag gcaagtggga tcgcgaccgc cctgtgcgcc
     5041 cgcctttgcg cggcccgctg gccagtcggg ccgaccatgc cacccgcatc ctgctgggcc
     5101 acagcgcgct gtgggaggcg ctgacgcatg aagaccacca gctgctgtgt gagctacccg
     5161 ccccccacgg cccgctgatg gcctggctgg aggcccaact acacgaacac ggtcccctgc
     5221 catggggcgc gctcaaggaa gccctggtgg accatgccga tgcagcgcat gccttgcgcc
     5281 tgatggccga tacccctatg ccgcccgaaa cggccagtga attcacgcac gagctgcgcg
     5341 atctgctcaa ccgcatgctg ctggagcaga tcgaacagca gaaaaaccag gccagcgcgg
     5401 cggccgccag cgatcccgcc gcgtttcaac gctaccggga attgaacgaa cgctggcagc
     5461 aactcaagca cagcatttcc tgagccagcc atgccattgg tcacgctgca gctgattcgc
     5521 cgactggaaa aatcccaaat cgacggtata atccaaggtt gattcggcaa gagcgacagc
     5581 agcacctccg ggccaggcca accgttgaca cagttcacga ccggccacct taccgcaagg
     5641 actgcatccc aaatgatcgc cctcccatct tgccatcggg gttaagcacc ccaccttgcc
     5701 aactacgccc gtcgtgcctg agacgtaggt ggtgtctgtt ctgtccgtcc gtcttttttt
     5761 gattctgagg ttcttgcatg cccgctcaaa aatctgccaa gcctgttcag tctgccgcca
     5821 aacctgcatc caagtccgcg tccaagaccg agaagaaagc ccctgccgtg ccagcatcca
     5881 aagcccctgt cgccaagtcc aaagccaccc tgttgaagga agctgctgac gcacttctgg
     5941 ccgcagacgc ccctgccaaa aagaaacctg gccgccctgc caaggccgcc actgccgaag
     6001 ccagcgcccc ccccaagaca ggcgccaagc gtggacgcaa gcccaaggcg gctgccgaat
     6061 cgtccggtga cgacatggac atgtccgaca tcgaggccga tctggctggc gaaccggtga
     6121 cgacaccggg cgaaaaggtc aaaccgctgc gcatgaagat cagcaaggcc aaagagcgcg
     6181 ccttgatgaa ggaattcggc ctggacgaaa ccgtcctgtc cgaagaagac atggccaagc
     6241 gccgcctgcg cctcaaggcc ctgatcaagc tgggcaagac ccgcggttac ctgacccacg
     6301 gcgaaatttc cgaccacctt cccgacaagc tggtcgatgc ggaaaccctg gaagtcgtga
     6361 tctccatgtt gaacgacatg ggcgtggcgg tgtacgaaca gacccctgac gcagaaaccc
     6421 tgctcctcaa caacaacggc cccaccgccg cgaccgaaga ggaagccgaa gaagaggctg
     6481 aagcggctct atctaccgtg gacagcgaat tcggccgcac caccgacccg gtgcgcatgt
     6541 acatgcgcga
//
LOCUS       DAOSJM010000104         6535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10727_length_6535_cov_4.349074, whole genome shotgun sequence.
ACCESSION   DAOSJM010000104 DAOSJM010000000
VERSION     DAOSJM010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10727_length_6535_cov_4.349074"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PKX82_05105"
     CDS             <1..319
                     /locus_tag="PKX82_05105"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOF51662.1"
                     /translation="FYTGLGVAPSLTGPNDALALLLFMMVLPLAGTFIGPLFAQLSRK
                     HEFEADAFAVAQTSGHDLSSALLKLYQDNASTLTPDPVYVKFYYSHPPASERLGRMLP
                     AAA"
     gene            340..663
                     /locus_tag="PKX82_05110"
     CDS             340..663
                     /locus_tag="PKX82_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOF51663.1"
                     /translation="MENTTPAPVRHALSATQVIAALSKLDGWRLHGDGADVAIEKTYT
                     FANYLQTMAFANAVAYLAEQQDHHPAMLVQWGSCSVRFNTHDVHGISKTDFECAALVD
                     ALAQG"
     gene            675..1607
                     /gene="rsgA"
                     /locus_tag="PKX82_05115"
     CDS             675..1607
                     /gene="rsgA"
                     /locus_tag="PKX82_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOF51664.1"
                     /translation="MAAKPQLEPGLVIAGHGRHVWVETPDGRRLICHPRGKKSQTVVG
                     DRVLWLATEDEGTIEKVEPRRNLFYRQDEIRTKSFAANLDQVLVLIAAEPEFSESQLS
                     RALIAAEAEHIRPIIALNKSDLVEPFGRAWDRMGIYRDMGYQVVPLALKPKSPGAVPT
                     DLDTLLPLMHGKTTLVLGPSGSGKSTLINRLLPDAQVLTNEISTALNSGKHTTTSTSL
                     YWVDPDKTTAVIDSPGFQEFGLNHIEPMHLAGLMPDIKAFAGECKFYNCTHLHEPGCG
                     VLSHMRSADSLQGISANRYKIYSDLFAELSQKRY"
     gene            complement(1615..2535)
                     /locus_tag="PKX82_05120"
     CDS             complement(1615..2535)
                     /locus_tag="PKX82_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HOF51665.1"
                     /translation="MSFISILLALLIEQARPLAQINPVHNMVRNWVRWVMRNFDAGKP
                     QHGWLAWGLSVLLPVTLVCLIHWLLVWTLGWVAAVVWNVAVLYATLGFRQFSHHFTAI
                     RDALHAGDEDQARQLLAQWMRMDASALPGSEIVRHVIEHSILSAHRHVFGVFAWYTLL
                     AALGLGPAGAVLYRVSEYVYTYANRPARPTSQPVSAAVVENASSAWAAIDWLPARVTA
                     LSFAFVGSFEEAVDSWRKQAARFPNDNDGVVLAATAGAVNVKLGPKEWDTIGQTPQPV
                     HLRAVVGLVWRTVVMWMVFLALLSLARLIG"
     gene            complement(2571..3245)
                     /locus_tag="PKX82_05125"
     CDS             complement(2571..3245)
                     /locus_tag="PKX82_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOF51666.1"
                     /translation="MTTPISFDPAQVPVTGVDTHLPKVSGELLAPAALRRRFVAPPVW
                     TPEYLSEPKFSQRTPVAASVLVPLVMRDQLMVLLTQRTAHLSSHSGQVAFPGGKADPE
                     DADVAATALREAQEEIGLHPDHVEVIGTLPIYTTGSAYIVTPVVALVQPDAPLVPNAD
                     EVADVFEVPLAFLMDPANHRHHAVDWQGATRQWLSMPYRDANRERFIWGATAGMLRNF
                     YRFLLD"
     gene            complement(3320..3691)
                     /gene="rplS"
                     /locus_tag="PKX82_05130"
     CDS             complement(3320..3691)
                     /gene="rplS"
                     /locus_tag="PKX82_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOF51667.1"
                     /translation="MNLIQILEQEEIARLGKTIPDFAPGDTVVVSVNVVEGTRKRVQA
                     YEGVVIAKRNRGLNSGFIVRKISSGEGVERTFQTYSPLIAGIEVKRRGDVRRAKLYYL
                     RDRSGKSARIKEKLPAKKTAA"
     gene            complement(3806..4552)
                     /gene="trmD"
                     /locus_tag="PKX82_05135"
     CDS             complement(3806..4552)
                     /gene="trmD"
                     /locus_tag="PKX82_05135"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOF51668.1"
                     /translation="MRFDVVTLFPELFEPFLKTGITRRAYEAGQVDVHLTNPRDYSSG
                     NYRRVDDRPFGGGPGMVMMAEPLAQALAAIRAARGDQAPVVLFSPIGQPLNHAAVQGW
                     AGSAGAILLCGRYEGIDQRFIDAYVDVQISLGDFVLSGGEIAAMALLDAVARLQPGVL
                     SDEDSHHQDSFNPALDGLLDCPHYTRPEVWNGVGIPAVLGSGHHENIERWRRDQRLLL
                     TAQHRPDLLDAARRAGALDSRDVRVLSGKS"
     gene            complement(4565..5128)
                     /gene="rimM"
                     /locus_tag="PKX82_05140"
     CDS             complement(4565..5128)
                     /gene="rimM"
                     /locus_tag="PKX82_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463704.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOF51669.1"
                     /translation="MLAGLEPAELPADAIEVGRVADAWGIKGWFRVLPYSADPEALFS
                     SKHWFLLPTEKGAQTFSGTAKLSIKEAKEHSDSVVATAHGVDDRNAAEALRGARIFIS
                     RSSFPTAAQDEYYWVDLIGLDVVNRQDEALGTVKELLSTGPQTVLVIAYQQDGKAMER
                     MIPFVSAYVDDVNLQERRIRVDWLADY"
     gene            complement(5179..5430)
                     /gene="rpsP"
                     /locus_tag="PKX82_05145"
     CDS             complement(5179..5430)
                     /gene="rpsP"
                     /locus_tag="PKX82_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOF51670.1"
                     /translation="MVVIRLARGGAKARPFFNIVVADKRTRRDGRFIERIGFYNPIAT
                     ANEESIRIAQDRLTYWKGVGAQASPTVERLINQAAKKAA"
     gene            5549..6073
                     /locus_tag="PKX82_05150"
     CDS             5549..6073
                     /locus_tag="PKX82_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOF51671.1"
                     /translation="MIKIRPSTDADVAAVTAIYAHHVLHGTGTFEITPPTEDDMATRR
                     GDVLSKALPYLVLEDNEGVAGFAYCNWFKPRPAYRFSAEDSIYLAERATGKGYGRLLL
                     QELMAQAERAGIRKLIAVIGDSANAGSIGVHRACGFEHVGILGACGWKFDRWLDVVLM
                     ERSLGLGSSQPPAP"
     gene            complement(6088..>6535)
                     /locus_tag="PKX82_05155"
     CDS             complement(6088..>6535)
                     /locus_tag="PKX82_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOF51672.1"
                     /translation="LGHGGNDAQKTIGIIWMMLIATGYVAAGDKSPPTWTIVCCYMAI
                     GAGTMFGGWRIVKTMGQRITKLKPVGGFCAETGGAMTLFLATALGIPVSTTHTITGAI
                     VGVGSTQRASAVRWGVAGNIVWAWVLTIPASAFVAAIAYWVSLQIF"
BASE COUNT         1399 a         2046 c         1884 g         1206 t
ORIGIN      
        1 gttctacacc ggcctgggcg ttgcgcccag cctgaccgga ccgaacgatg cgctcgcact
       61 gctgctgttc atgatggtgc tgccactggc aggcaccttc atcggcccac tgtttgctca
      121 gctgtcgcgc aagcacgaat ttgaagcaga tgcctttgcc gtggcacaaa ccagcgggca
      181 cgatctgtcc agcgccctgc tcaagctcta ccaggacaat gcctctaccc tcacgcccga
      241 cccggtgtac gtgaagttct attactcgca cccgccggcg tccgagcgcc tggggcgcat
      301 gctgcctgcg gccgcctgaa ctccatgcca aagaaattta tggaaaatac cactccagcc
      361 cccgtccgcc atgcgctaag cgctacacaa gtgatagcag ctctatccaa gctggacggc
      421 tggcgattgc atggcgatgg cgcagacgtt gccatcgaaa agacctacac cttcgccaat
      481 tacctgcaga ccatggcttt cgccaacgcg gtggcctatc tggccgagca gcaggaccac
      541 catccggcga tgctggtgca atggggcagt tgcagcgtgc gcttcaacac ccatgatgta
      601 catggcatct ccaaaaccga tttcgaatgt gctgcgctgg tcgatgcgct ggcgcagggt
      661 tgaggaccgc cgcattggcc gccaaacccc agcttgaacc cggcctggtc atcgcggggc
      721 atggccgcca tgtgtgggtg gaaacacccg atggccgccg actgatctgc catccgcgcg
      781 gaaagaaaag ccagaccgtg gtcggggacc gagtgctgtg gctggccact gaggacgaag
      841 gcaccatcga gaaagtagag ccccgtcgca acctgttcta ccgccaggac gaaattcgca
      901 ccaagtcgtt tgccgccaat ctggaccagg tgctggtgct catcgctgcg gaacccgagt
      961 tctccgagag ccagctctca cgtgcgctga ttgcggctga agccgaacac attcggccca
     1021 tcattgcgct caacaagagc gacctggtcg aaccctttgg ccgcgcatgg gaccgcatgg
     1081 gcatctaccg cgacatggga taccaggtcg taccactggc acttaaaccc aaatctccag
     1141 gcgccgtgcc gaccgatctg gatacgctgc tgccgttgat gcatggcaag acaacgctgg
     1201 tgctggggcc ctccggctcc ggaaaaagca cgctgatcaa ccggctgctg cccgatgccc
     1261 aggtgctcac caacgagatt tcaaccgcac tgaactcggg caagcacacc acgaccagca
     1321 ccagcctgta ttgggtggac ccagacaaaa ccacagcggt gatcgattca cccgggtttc
     1381 aggagttcgg tctcaaccat attgaaccga tgcacctggc cggcctgatg cccgacatca
     1441 aggcctttgc gggggagtgc aagttttaca actgcacgca cctgcacgag cccggttgtg
     1501 gtgtactttc ccatatgaga tcggcagata gccttcaggg aatcagcgcg aatcgctaca
     1561 aaatttatag cgatctgttt gcggagctat cgcaaaaacg ctactgacag cacgtcatcc
     1621 gatcagcctg gccagcgaca gcaaggccaa gaacaccatc cacatcacca cggtgcgcca
     1681 caccagtcca accacggcgc gcaggtggac tggctgtggt gtctgcccga tagtgtccca
     1741 ctcctttggc cccagcttga cattcactgc gccagccgtg gcagccagca ccacgccatc
     1801 gttgtcgttc ggaaaacgtg cagcctgctt gcgccagctg tccaccgcct cctcaaagct
     1861 gccgacgaac gcaaaactca gggcggtcac ccgtgccggc aaccagtcaa tggcagccca
     1921 ggcactgctg gcgttttcca ccacagcggc actgacgggc tggcttgtcg ggcgcgcagg
     1981 ccggttggca taggtgtaga catattcact gacccggtac agcacggcac cggcgggccc
     2041 cagccccaag gctgccagca gggtgtacca ggcaaataca ccgaacacat ggcggtgcgc
     2101 cgacagaatg gagtgctcga tgacatggcg gacaatctcg ctgcccggca gtgcactggc
     2161 atccatgcgc atccactgtg ccagcaactg gcgggcctgg tcttcatccc ccgcgtgcag
     2221 tgcatcgcga atggcagtga aatggtggct gaactgccga aagcccagcg tagcgtagag
     2281 gacggcgacg ttccagacca cggcagcaac ccatcccaag gtccagacca gcagccagtg
     2341 gatcagacac accagcgtga cgggtagcaa caccgacagt ccccacgcca gccagccgtg
     2401 ctggggcttg cccgcatcga aattgcgcat cacccatcgc acccagttgc gcaccatgtt
     2461 gtgtaccggg ttgatctggg ccaggggtct ggcctgctca atcagcaagg ccagcagaat
     2521 ggagatgaaa ctcatggagc aatcatatag cggccctacc cgcgtcaaac ctagtccagc
     2581 aggaagcggt agaaattgcg cagcatgccc gccgttgcac cccagatgaa acgctcacgg
     2641 ttcgcatccc ggtagggcat ggatagccac tggcgcgtag ctccctgcca gtccaccgca
     2701 tggtgccggt ggttggccgg gtccatcaga aaggccagag gcacctcgaa cacatcagcc
     2761 acttcatcgg cgttgggaac cagcggtgca tccggctgca ccagcgccac cacgggtgtg
     2821 acgatatagg cactaccggt ggtgtagatc ggcaaggttc cgatgacttc cacgtggtct
     2881 ggatgcaggc caatctcctc ctgcgcctcg cgcagcgcgg tggcggccac atccgcgtct
     2941 tcggggtcag ccttgccgcc tggaaaagcg acctgcccgg aatggctgga gagatgcgcg
     3001 gtacgctgtg tcaacagcac catcaactgg tcgcgcatga ccagcggcac caagaccgat
     3061 gcagccacgg gtgtgcgctg cgaaaacttg ggctcggaca aatactccgg cgtccatact
     3121 ggcggtgcta caaaccgcct gcgcaatgcc gcgggggcca acagctcccc cgaaaccttg
     3181 ggcaagtggg tatccacacc ggtcaccggg acctgcgcag ggtcaaagga aatgggggtg
     3241 gtcacgttac gttcagacaa caaaaaagcc gccgcagtgc aatgctgggg cggcttctgc
     3301 tgaaaacagc ctgctgaaat tacgcagcag tcttcttcgc tggcagtttt tccttgatac
     3361 gtgcagactt gccgctacgg tcacggaggt agtacagctt ggcgcggcgc acatcaccgc
     3421 ggcgcttgac ttcgatgccg gcaatcaggg ggctgtaggt ctggaacgta cgctccacgc
     3481 cttcgccgct ggaaattttg cgaacgataa agccgctgtt gaggccacga ttgcgcttgg
     3541 caatcacgac gccttcgtag gcctgcacgc gcttgcgggt gccttcaacc acgttgacgc
     3601 tgacaaccac ggtgtcgcca ggtgcgaagt cgggaatggt tttaccgagg cgagcaattt
     3661 cttcttgctc aagaatttga atcaggttca tagcttcttt cgtgatcttg cccgcgtcag
     3721 caccggaatt ggcgctatcc gcacgccatc aaagacatgc gacctgaccg gacagaggat
     3781 cggttagcct gctattgtag caggctcagg acttgcctga caatacgcgc acatcccggg
     3841 aatccagggc gccggcccgg cgtgcagcgt ccagcagatc ggggcggtgt tgggcggtca
     3901 gcaaaagtcg ctgatcccgg cgccagcgct cgatgttttc atggtggccc gaacccagta
     3961 ccgcaggtat gcccacgcca ttccagacct cagggcgtgt gtaatgggga cagtcgagca
     4021 ggccatccag cgctggatta aagctatcct ggtggtggct gtcttcatcg gacagcacac
     4081 caggttgcag gcgagctaca gcgtccagca gcgccatcgc ggcaatttca ccccccgaaa
     4141 gcacgaagtc cccgaggctg atctggacat ccacgtaagc atcaatgaag cgctggtcaa
     4201 tcccctcgta ccggccacag agcagaatgg caccggcact acccgcccac ccctgcacgg
     4261 cagcgtggtt caggggttgc ccaatgggtg aaaacaggac caccggagcc tggtccccac
     4321 gtgccgcacg gatggctgcc aatgcctgcg ccaggggctc agccatcatc accatgccgg
     4381 gaccaccccc gaagggccgg tcatcgaccc ggcggtagtt gccgctggag taatctctgg
     4441 gattggtcag atgaacatca acctggcccg cctcataggc ccgccgggta atgcctgtct
     4501 tcagaaaggg ctcgaagagc tcaggaaaca gggtaacaac gtcaaaacgc atcgaacggc
     4561 cggatcaata gtccgccagc cagtcgacac gaatgcgccg ctcctgcaga ttcacgtcat
     4621 ccacataggc ggatacaaat ggaatcatgc gctccatcgc cttgccatcc tgctggtaag
     4681 cgatcaccag caccgtttgc gggccggtag acaacagctc cttgaccgtg cccaatgctt
     4741 cgtcctggcg attgaccacg tcaagaccga tcaggtcgac ccagtagtac tcgtcttggg
     4801 ccgcggtagg aaagctggag cgggaaataa agatacgggc gccgcgcaag gcctcggcgg
     4861 cattgcgatc atcaacacca tgcgcggttg ccaccacgga gtctgaatgc tccttggctt
     4921 ccttgatgga gagcttggcg gttcctgaga aggtctgtgc gcccttctcg gttggcaaca
     4981 ggaaccagtg cttggacgag aacagcgcct caggatcggc actgtagggc aaaaccctga
     5041 accagccctt gataccccac gcatccgcca cccgcccgac ttcgatggca tccgctggca
     5101 gttcagcagg ttcgagtccg gcaagcatgg tgtatgggat tcaagccagg aaggtgggca
     5161 atagacccac ctccaggatc aggcagcctt cttggcagcc tggttgatca ggcgttccac
     5221 ggtgggggaa gcctgtgcgc caacgccttt ccagtatgtc aggcggtcct gagcgatacg
     5281 gatgctttct tcgttggccg tagcgatcgg gttgtaaaaa ccgatacgct cgatgaagcg
     5341 gccgtcgcgg cgggtacgct tgtcagcaac aacaatattg aaaaacgggc gagcttttgc
     5401 accgccacgg gcgagtcgaa tgacgaccat gatttatcct ttgggtagct gaaagatttc
     5461 agccttgaat tcaaagtttg agacacgcaa ctgaccaccc ggccagtgac actctgaata
     5521 gccttctatt ataacgtttt caatacccat gatcaaaatt cgccccagta ccgatgccga
     5581 tgtggctgct gtcaccgcca tttatgccca ccatgtgctc catggcaccg gcacattcga
     5641 gattacccct cccacagagg acgacatggc gacgcgacgc ggtgatgtgc tgtccaaggc
     5701 cctgccctac ctggtgctgg aagacaacga aggcgtcgct ggatttgcgt actgcaactg
     5761 gttcaagccc cgcccggcct accggttttc agcagaagac tccatctatc tggcagagcg
     5821 agccactggc aagggctatg gacgcctgct gctgcaggag ctgatggcac aggcagaaag
     5881 agccggtatt cgcaaactca ttgcggtgat tggtgactct gcaaacgcag gctccatcgg
     5941 cgtacaccgc gcctgtggct ttgagcacgt aggcattttg ggtgcctgtg gctggaaatt
     6001 tgaccgttgg ctcgacgtcg tcctgatgga gcgctcgctg ggcttgggct cgtctcagcc
     6061 gccggcccca tagcgcctga ctgctcgcta gaagatctgg agactgaccc agtaagcaat
     6121 ggctgcaaca aacgcgctgg ccggaatggt cagcacccaa gcccacacaa tattgcccgc
     6181 cacaccccag cgcaccgcgc tggcgcgttg ggtggaaccc acacccacaa tcgccccggt
     6241 aatggtgtgt gtggtggaaa ccgggatacc cagagccgtt gcaaggaaca gtgtcatcgc
     6301 cccacccgtt tcggcgcaaa atccgccgac aggtttgagc ttggtgatgc gctgtcccat
     6361 cgtcttcaca atgcgccagc cgccaaacat ggtgccagcg ccgatggcca tgtagcagca
     6421 cacgatggtc caggtcggtg gcgacttgtc gccggctgcc acatagcccg tggcaatcag
     6481 catcatccag ataatgccaa tcgtcttctg ggcgtcattg ccgccgtgcc cgagg
//
LOCUS       DAOSJM010000105         6522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10741_length_6522_cov_4.657492, whole genome shotgun sequence.
ACCESSION   DAOSJM010000105 DAOSJM010000000
VERSION     DAOSJM010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10741_length_6522_cov_4.657492"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="PKX82_05160"
     CDS             complement(<1..630)
                     /locus_tag="PKX82_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOF51673.1"
                     /translation="MTVVLGAVPVVGQAQQPTYNFSPVNQWDIKLTAAYWNPIIAYVS
                     EKSGVKLNLKIGRTSADTTSYVLANEVEFVFSNHLFSPQREQLGWKVFGRRNTPPLQG
                     QIVVPVDSSITDLAQLKGQEVAFAGPEAFVIYKVPYAHLLAKGIEVKPVFAGNQNGAF
                     AQLFSGKVVAAGGNSQLVDGYSRREGKKFRVLWSSEPFQDLALMASGKVP"
     gene            complement(742..1545)
                     /gene="rsmA"
                     /locus_tag="PKX82_05165"
     CDS             complement(742..1545)
                     /gene="rsmA"
                     /locus_tag="PKX82_05165"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOF51674.1"
                     /translation="MKHIARKRFGQHFLSDGGIIDAIVHEIAPKPGQHMVEIGPGLAA
                     LTQPLVERLGHLTVIELDRDLAQRLRSHGQLTVIESDVLKVDFSSILPSSSAPETGAN
                     AMAAPAAHKLRVVGNLPYNISTPILFHLLQYVDAIEDQHFMLQKEVVDRMVAKPATSD
                     YSRLSVMLQWRYAMEDVLFVPPESFDPPPRVDSAVVRMVPLAVPPKVNPIFLGELVQV
                     AFSQRRKLLRHTLGKWLEQRAYSGHFDVQRRAEEVPVAEYVELAQGLQA"
     gene            complement(1542..2849)
                     /locus_tag="PKX82_05170"
     CDS             complement(1542..2849)
                     /locus_tag="PKX82_05170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF51675.1"
                     /translation="MNLRLWALALACISGIGGAAAQVQASRQADFIVAVVNSEPITNS
                     EVRAQVQRVSAQITQQGQKLPSPEELRRDALERLINERAQLQMARETGIRVDDAAVDQ
                     AEQMVASQSQIDVAELRKRVVKEGTDLATFRTQLRERVLISRLHERDIESRVRVTDQD
                     IDRYLQEQQASNTDPFAQEINLAQLLVAVPEKASSEQAALLYQQAQKVLNRIRAGEDF
                     GTLVKELSAADRNNGGQLGLRRADRYPPSFVLATQKLGVGGVSDIVRSAAGFHILKVV
                     DRRAPAVLVRTVVQSRARHILLRTGPELTAAQAQARLADYRQRILGGKADFQKLAREY
                     SQDGSAQQGGDLGWVSQGTFVPEFEEAMNRLNEGEISNPVVSRFGVHLIQLAERRRVD
                     LSPQELRQMVGNQLRETKYAEAYVVWARDVRERAYVEMRDPPQ"
     gene            complement(2857..5250)
                     /locus_tag="PKX82_05175"
     CDS             complement(2857..5250)
                     /locus_tag="PKX82_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HOF51676.1"
                     /translation="MRFSPSEPLALQILHHRQPLRPVTLLVCSVLCSGAFAQDAAEPV
                     AESLALQPSRLLEEKISAEQRDQAPTFLQGDRISGRPDLETVIEGDATLRKAGTTIRA
                     DRLEYDQPTDQAKATGNVRINRGGNVYEGPQLELKVERFEGFFNQPRYQFLQNDAHGE
                     ADRIDFLDESHAVIHNATYTTCRRLPGPSWLPDWILRAATITLDNDEDVGVATGAVLR
                     FKGVPILPVPAVSFPLSDKRKSGVLPPTIGVDNVNGAEVVVPYYWNIAPNRDATITPT
                     LMTLRGVDLGAEFRYLEPSYSGTVRANYMPADKLRSADRWGMSYLHAGSIDTGLKDVG
                     QVGLSVNLNRVSDDNYWKDFTRSSSTLTQRLLPNDVNASWGRGFVSVGARVLQWQTLQ
                     DPTAPIVPPYDRVPQLTARYAQTNLQGLDFSVDGDFTQFEADRFKTLQPNTQRTYTQL
                     QVSRPWLAPAGFITPKLQLHAAAYQFDTMLANGSNTADSVVPTFSLDSGLVFERDATL
                     WGRSLVQTLEPRAFYVYTPYRNQSALPNYDTGANDFNFASIYTENAFVGHDKISDNNL
                     LTLGLTTRLLDEQTGAQIARFGIAQRLRFEDQLVTLNSATAPSLAGFSDVLLGAAVNV
                     NDRWALDSTVQYNAKTDQSVRSTVGARYSPGSYRVINTAYRFQRGASEQIDMSWQWPL
                     NDLWGDRGQELGAGRGQGPGRYYSVGRMNYSLNEGRLVDTVLGVEYDAGCWLGRVVFE
                     RVQTSTSSATERLMFQLEFVGFTRLGVDPRRTLTQNISRYQNLRDTGGSNSRFSNYD"
     gene            5286..6380
                     /locus_tag="PKX82_05180"
     CDS             5286..6380
                     /locus_tag="PKX82_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOF51677.1"
                     /translation="MSSTPTSPSPSSSSVTWADAARQTQFEQWFASVQTAQGLDRTTL
                     QLASADASFRRYLRVDSAQGSRIIMDAPPDKENCQPFVHVAQLMETAGLKAPQVLAWD
                     EPHGFMLLSDLGNQTMMQVIDPAAPPLALYLQAVDVLVQWQLASRPGELPPYDEALLR
                     RELELFPQWYLTEHRKVAIDSAMRKTLDDAFACIIQNNLAWPSVYVHRDFMPRNLMVA
                     PSTTGDTSLGVLDFQDAVYGPITYDIASLMRDAFLSWEEDFCLDVTIRYWEKARKAGL
                     PVGDDFGEFYRGVEWMGLQRHLKVAGIFARLTLRDGKPHYLADTPRFIGYIRSTCARY
                     RELKPLLRLVESVEGIEPSNILAYNKPQAK"
BASE COUNT         1245 a         2104 c         1888 g         1285 t
ORIGIN      
        1 cggcaccttg cccgaggcca tcagtgccag atcctgaaat ggctcagaac tccacagcac
       61 ccggaatttc ttgccctcgc ggcgggagta tccatccacc aactgggaat tgccacctgc
      121 agcaaccacc ttgccgctga acagctgcgc aaaagcacca ttctgattgc cggcaaacac
      181 cggcttgact tcaatgcctt tggccagcaa gtgggcatag ggcactttgt aaatgacaaa
      241 cgcttcaggc cccgcaaatg cgacttcctg ccccttgagc tgggccaggt cggtaatgct
      301 cgaatcgaca ggcaccacga tctgtccctg cagtggcggg gtgttgcgcc ggccgaagac
      361 cttccacccc agttgctcac gttgtggact gaaaaggtgg ttgctgaaca cgaattccac
      421 ttcgttggcc agcacatagc tggtcgtgtc cgccgaggtc ctgccaatct tcaggttgag
      481 cttgaccccg ctcttttctg aaacatacgc aatgatggga ttccagtacg ccgccgtgag
      541 tttgatatcc cactgattca ctggagagaa gttgtaggtc ggctgctgcg cctgccccac
      601 gacaggtacc gcccccagca ccactgtcac aactgcggca ccagcgatcc ggaaaaattg
      661 ccttctcatt tccacgacca tttcccttgt tgataaagcg cttcgcgcac tctggatgcc
      721 atcataggac agccctgaac ctcaggcttg caggccctgc gcaagttcca cgtactctgc
      781 aaccgggact tcctccgcgc gccgctgtac atcgaagtgc ccggaatacg ctcgctgctc
      841 cagccacttt cccagcgtgt gccgcaacag cttgcgacgc tggctgaagg ccacctggac
      901 cagttcaccc agaaagatcg ggttcacctt cggcggtacc gcgagtggca ccatacgcac
      961 caccgcactg tccaccctgg ggggtggatc aaaactctct ggtggcacaa acagcacatc
     1021 ctccatggca tagcgccatt gcagcatcac gctcaggcgg ctgtagtccg aggttgccgg
     1081 ctttgccacc atgcggtcca caacttcctt ctgcagcatg aagtgctggt cctcaatggc
     1141 atccacgtac tgcaacagat gaaagagaat cggtgtagaa atgttgtagg gcaggttacc
     1201 caccacccga agcttgtgcg ctgccggcgc agccattgcg ttcgctcctg tttcaggagc
     1261 actggaagac ggcaggatgc tggaaaagtc cactttcagc acatccgact cgatcaccgt
     1321 cagctgccca tggctgcgca gacgctgcgc gagatcgcgg tccagctcaa tcactgtcag
     1381 gtgcccgagc cgctctacca gtggctgggt cagtgcagcc agacccggcc cgatttccac
     1441 catgtgctgc cccggtttgg gcgcaatctc gtgcacgatg gcgtcgatga tgccgccatc
     1501 cgacaggaag tgctggccaa accgtttgcg tgcaatgtgc ttcattgcgg agggtcgcgc
     1561 atttccacat acgcgcgctc gcgtacgtca cgcgcccaca cgacataggc ctccgcgtac
     1621 ttggtttcgc gcaactggtt gcccaccatc tggcgcaatt cctggggact aagatccaca
     1681 cggcggcgtt ctgcgagctg gatcaggtgc acaccaaagc gcgataccac cggattgctg
     1741 atctcgcctt cgttgagccg gttcatggcc tcttcaaact ccggaacaaa agtaccctgg
     1801 ctgacccacc ccaggtctcc gccctgctgg gcactgccat cctgggagta ttcgcgcgcc
     1861 agcttctgga aatcggcctt gccgccgaga atgcgctgcc tgtaatcggc caaccgtgcc
     1921 tgggcttgtg ccgccgtcaa ctcgggacct gtacgcaaga gaatatgccg tgcacggctc
     1981 tgaaccacgg tgcgtacgag tacggccggt gcacggcggt ccacgacctt gaggatgtga
     2041 aagcccgccg cagaacgcac gatgtcggac acaccgccca cccccagctt ctgggtcgcc
     2101 agaacaaacg acggtggata tcggtccgcc ctgcgcagtc ccagttgccc accgttgttg
     2161 cgatcagccg cagacagttc cttgaccaga gtgccaaaat cttcccccgc acgaatgcgg
     2221 ttcagtacct tctgggcctg ctggtacagc aacgccgcct gctcggagga agccttctcc
     2281 ggcacggcca ccagcaactg cgccaggttg atttcctggg cgaaaggatc ggtgttgctg
     2341 gcctgctgtt cctggagata gcgatcaata tcctgatccg tcacccgcac acgggactca
     2401 atatcgcgct catgcaggcg gctgatcagc acacgttccc gcaactgcgt gcgaaacgtg
     2461 gccagatccg tgccctcttt gacgacacgc ttgcgcaatt ccgccacgtc aatctgactc
     2521 tggctggcga ccatctgttc ggcctggtca accgcagcgt cgtccacccg aatgccggtt
     2581 tcacgggcca tctgcagctg cgcgcgctca ttgatcagtc gctccagcgc atcgcgccgc
     2641 aactcctccg gtgacggcaa tttctgccct tgctgggtga tctgcgcgct cacccgctgc
     2701 acctgtgcac ggacttcgct gttggtgatg ggctcggagt tcaccacggc gacgatgaag
     2761 tccgcctggc gggatgcctg cacttgcgcc gctgcgccgc caatgccact gatacaagca
     2821 agggccaaag cccacaagcg aagattcatg ttttgtttag tcgtagttgc tgaagcggct
     2881 gttgctgccg cctgtgtcgc gcaaattctg gtaacgcgaa atattctgtg tcaaagttcg
     2941 gcgtgggtcc acgcccagcc gggtaaagcc cacaaattcc agctggaaca tcaggcgctc
     3001 ggtcgcagag gatgtactgg tctgcacccg ctcaaagacc acgcgcccca gccagcagcc
     3061 cgcatcgtac tcgaccccca gtacggtgtc gaccaggcga ccttcgttca ggctgtagtt
     3121 catccggccc acgctgtagt agcgacccgg cccctggccg cggccggcac ccagctcctg
     3181 cccgcggtcc ccccacaggt cattgagagg ccactgccag ctcatgtcaa tctgttcgct
     3241 cgcaccgcgc tggaaacggt aggccgtgtt gatgacgcgg tagctacccg ggctgtagcg
     3301 cgctccaacg gtggagcgca cggactggtc ggttttggcg ttgtactgca cagtggaatc
     3361 caacgcccag cggtcattaa cgttgacagc cgcgcccaac aatacatcgc tgaagcccgc
     3421 cagtgaaggc gcagtcgcac tgttgagcgt caccagctgg tcttcgaagc gcaggcgctg
     3481 ggcgatgcca aaccgtgcaa tctgtgcacc ggtctgttca tccagaagac gggtggtcag
     3541 ccccagggtg agcaggttgt tgtccgatat cttgtcgtgc cctacaaagg cattctcggt
     3601 atagatgctt gcaaaattga agtcgttcgc accggtgtcg taattgggca gggcactctg
     3661 gttgcggtag ggcgtgtaga cataaaacgc gcggggctcc agcgtctgca ccagggaccg
     3721 tccccagagc gtggcgtccc gctcgaacac cagaccactg tccaggctga aggtcggcac
     3781 cacgctgtct gcggtgttgc tcccgttggc cagcatcgtg tcaaactggt aggccgccgc
     3841 atgcagctgg agcttcgggg tgatgaagcc tgcgggtgcc agccaggggc ggctgacctg
     3901 caactgggtg taggtgcgct gggtattggg ctgcagggtt ttaaaccggt cggcctcgaa
     3961 ctgggtgaag tcaccgtcca ccgagaaatc caagccctgc agattggtct gggcataccg
     4021 cgccgtcagt tgtggcacac ggtcgtacgg cggcacgatg ggcgccgtgg ggtcctgcaa
     4081 ggtctgccac tgcagcaccc gggcaccgac gctgacaaag cctcgccccc aggaggcatt
     4141 cacgtcgttg ggcagcagcc gctgtgtcaa ggtggaactg ctgcgggtga aatccttcca
     4201 gtagttgtcg tcactgacgc ggttcaggtt gaccgacagc cccacctggc caacgtcctt
     4261 caggccggtg tcaatgctgc cggcgtgcag atagctcatg ccccagcggt ccgcactgcg
     4321 cagcttgtcc gccggcatgt aattggcccg caccgtgccg ctgtaggacg gctccaggta
     4381 gcgaaactca gcacccaggt ccacgccacg cagggtcatc agggtggggg tgatggtggc
     4441 atcccggttg ggtgcaatgt tccagtagta gggaacgacc acttcggcgc cattgacgtt
     4501 gtccaccccg atggtaggcg gcaacacacc ggacttgcgc ttgtcgctca acggaaaact
     4561 gactgccggc accggcagaa tgggcacccc cttgaagcgc agcaccgcgc ccgtggcaac
     4621 cccgacatcc tcatcgttgt ccagcgtgat ggtggcggcg cgcaatatcc agtcgggcag
     4681 ccagctcggt cccggcaggc ggcggcaggt ggtgtaggtc gcgttgtgga tgacggcatg
     4741 gctttcatcc agaaagtcga tacggtccgc ctcgccatgg gcatcgttct gcagaaactg
     4801 gtagcgcggc tggttgaaaa aaccttcaaa ccgctccacc ttgagttcga gctgggggcc
     4861 ttcgtacaca ttgccgccgc ggttgatgcg cacgttgcca gtcgccttgg cctggtcggt
     4921 cggctggtca tactcgagcc ggtcggcgcg gatggtggta ccggccttgc gcaaagtggc
     4981 atccccctcg atcaccgtct ccaggtccgg ccggccggag atgcgatcgc cctgcagaaa
     5041 ggtcggcgcc tggtcgcgct gttccgcgct gatcttttcc tccagcaggc ggctgggttg
     5101 cagcgccaga ctctcggcga cgggttctgc agcgtcctgc gcaaacgcac ccgagcacag
     5161 cacgctgcac accagcaggg tgactggacg cagcggttgc cggtggtgaa gaatctgcaa
     5221 agccagaggc tcgctgggag aaaagcgcat gaggacgact ggcgggtttg tagaatggat
     5281 tatccatgag ctccacgcct acttctccct ccccgtcttc ctcctccgtt acatgggccg
     5341 atgccgcgcg acaaacccag ttcgaacaat ggtttgccag cgtacagacg gcgcaaggcc
     5401 tggaccgcac caccttgcag ctggcctcag cagatgctag ctttcgacgc tacctgcgcg
     5461 tggacagcgc acagggcagc cgcatcatca tggatgcccc gcccgacaag gaaaactgcc
     5521 agccgtttgt gcatgtcgca cagctgatgg agaccgctgg cctgaaggcg ccccaggttc
     5581 tggcctggga tgagccgcat ggtttcatgc tgctgtctga cctgggcaac cagaccatga
     5641 tgcaggtaat cgaccccgct gcgcccccac tggccctgta cctgcaggcg gtggacgtac
     5701 tggtgcaatg gcaactggcc tcccgacccg gtgagctgcc gccttatgac gaagccctgc
     5761 tgcgccgtga actggagctg tttccccagt ggtacctgac cgaacaccgc aaggtagcca
     5821 tcgactccgc catgcgcaag acactggatg acgcctttgc ctgcatcatc cagaacaacc
     5881 tggcctggcc cagcgtgtat gtgcaccggg acttcatgcc gcggaacctg atggttgctc
     5941 catccaccac cggcgatacc tctctaggcg tgctggactt ccaggacgcg gtttacggcc
     6001 ccatcaccta tgacattgcc agcctgatgc gcgacgcctt cctgagctgg gaagaggatt
     6061 tttgtctgga cgtgaccatc cgctactggg aaaaagcccg caaggccggt ctgcccgtcg
     6121 gtgatgactt tggtgagttc taccgtggcg tggaatggat gggcctgcag cgccacctca
     6181 aagtggccgg catctttgca cgcctgacac tgcgcgacgg caaaccacac tacctggccg
     6241 acacgccgcg ctttattggc tatatccgct ccacctgcgc ccgctaccgg gaactcaaac
     6301 cgctgctgcg cctggtggaa tcggtcgaag gcatcgaacc ctccaacatc ctggcctaca
     6361 acaaacctca agcaaaatag gctcctagtc cgcgcagaat atgcgcagtc agctatatat
     6421 ttgatagcaa acaattccgt tgccatgccc cgttttttct gccccgctgc acttgccacc
     6481 gatgacctgc tggacctgcc cgcgggcgcg gcacgccatg tg
//
LOCUS       DAOSJM010000106         6522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10742_length_6522_cov_3.315448, whole genome shotgun sequence.
ACCESSION   DAOSJM010000106 DAOSJM010000000
VERSION     DAOSJM010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10742_length_6522_cov_3.315448"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="PKX82_05185"
     CDS             <1..270
                     /locus_tag="PKX82_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF51678.1"
                     /translation="GRALMSRPKVLLLDEPSMGLSPIMVDKIFEVIKDVYAQGVTVLL
                     VEQNASRALSIADRGYVMESGIVTMTGDAKHMLNDPKVRAAYLGE"
     gene            263..1114
                     /locus_tag="PKX82_05190"
     CDS             263..1114
                     /locus_tag="PKX82_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF51679.1"
                     /translation="MNRHVLALLFVTLVWGATFPVLKLATAHLSGVEISALRFVIAAL
                     CMAPWALRASCRAWTDGAVLGALVLVSYVAQAWGLEHISSNRSAFLTSLNVLMVPLLG
                     IVLGNRLSWTVLLAAAMACVGIGLMSWDGGAHLLADSATVFGALAYAGYVIFLSRKAG
                     LHSARTLAATQIVWMALLGSVWAWAASLGTDRMHTLPERIDTQILLGLAYLGVVATAG
                     MLFLQAMAQRHVSADKAALIYAMEPVFAALFAWIWLAESLSWMAAFGAVIVVSAVLLS
                     EWKSGAG"
     gene            complement(1186..2238)
                     /locus_tag="PKX82_05195"
     CDS             complement(1186..2238)
                     /locus_tag="PKX82_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HOF51680.1"
                     /translation="MTATVTSVSSFSVTVQPSGRQFSAQADETVLAAGLRQNIGLPYG
                     CKDGACGSCKCKKTSGSVTHGTHQSKALSTEEEAAGYVLTCCATPQSDLVLESRQVTE
                     AGAIPIKKLPTRVSTLTRLTDDVVLMQLQLPANDNFTYRPGQYVEILLRDGARRSYSM
                     ASVPQAGAGLELHIRHMPGGKFTDHVFGAMKEKEILRIEGPYGSFFLREDSDKPIILL
                     ASGTGFAPIKTLIEHLKQTGSSRAISLYWGGRRPSDLYMDSWVQAQCETMPTLQYIPV
                     VSDALPQDVWAGRTGFVHQAVLQDHPDLSGYQVYACGAPIVVDSARRDFCATAGLPEE
                     EFYADAFTSEADKQQG"
     gene            2282..3202
                     /locus_tag="PKX82_05200"
     CDS             2282..3202
                     /locus_tag="PKX82_05200"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF51681.1"
                     /translation="MPSIQSPLGALPSRFRRERVLIVGCGDVGQRVAGLLRRNVRVVA
                     LTSSTARVSELRAQGVVPLLGNLDNPDSLRRLAGISTRVVHLAPPPAHGWQDLRTQAL
                     LQVLRLRTSPAACVYGSTSGVYGDCAGAWIDETRTTNPDSPRARRRQHAEDCVRFFGR
                     SAGIRTHTLRIPGIYAPDREGGTPRARLLKRTPVLCAEEDVYTNHIHADDLARACVLA
                     MWRGRAQRVYNTNDDTALKMGDYFDLAADLYDLPRPPRVSRAGATEQLPLMLLSFMGE
                     SRRMVNRRLKQELGLRLRYPTVREGLVSLA"
     gene            complement(3163..3882)
                     /locus_tag="PKX82_05205"
     CDS             complement(3163..3882)
                     /locus_tag="PKX82_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOF51682.1"
                     /translation="MPATPSNPEQFGKTPTLYFWVWGISVLLALIPTVWTTLDLQAAS
                     LFAGPSPAVASIGWWWVELINLYVPAAFRVGIALALVVWLVASFRKQGKPLRLAMAFL
                     VLAGILGPGAVVNLGFKDHWQRARPYQVENFGGTQQFTRAAVMTNQCDANCSFVSGHV
                     ACGFFFTSLMLIHRKRKVAWAVTGTLAGWVIGFARMSDMAHWFSDALWAYPITLASSW
                     LVWKALHWAYAKETSPSRTVG"
     gene            3988..5067
                     /gene="rfbB"
                     /locus_tag="PKX82_05210"
     CDS             3988..5067
                     /gene="rfbB"
                     /locus_tag="PKX82_05210"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOF51683.1"
                     /translation="MTILVTGGAGFIGSNFVLDWLSQSQEPVVNLDKLTYAGNLENLA
                     SLDGDARHIFVRGDIGDSALVSGLLAQHQPRAIVNFAAESHVDRSIHGPEDFIQTNIL
                     GTFHMLECVRAYWGALPTEAKGAFRFLHVSTDEVYGSLSKTDPAFTEENKYEPNSPYS
                     ASKAASDHLVRAYHHTYGLPVLTTNCSNNYGPFHFPEKLIPLMIVNAQAGKPLPVYGD
                     GQQIRDWLYVKDHCSAIRRVLDAGQLGETYNVGGWNEKPNLDIVHTVCALLDELKPRA
                     DGKPYQKQITYVTDRPGHDRRYAIDARKIEQQLGWKPAETFETGIRKTVQWYLDHPQW
                     VANVLSGGYRDWVEKQYGDSAKSVA"
     gene            5064..>5701
                     /gene="rfbD"
                     /locus_tag="PKX82_05215"
     CDS             5064..>5701
                     /gene="rfbD"
                     /locus_tag="PKX82_05215"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOF51684.1"
                     /translation="MKILLLGRDGQVGWELQRSLAPLGEVVALGRDTAANPQGLCGDL
                     TQLEDLAATVRAVAPNVIVNAAAHTAVDKAESEVDLARVINAVAPGVLADEAQRLGAW
                     LVHYSTDYVFDGSGEAPWKETDGTGPLSVYGRTKLAGEQAVARCDKHLVFRTSWVYSA
                     RGGNFARTMVRLAQTRELLQVINDQYGAPTGADLLADVTAHAIRQVAYHGATG"
     assembly_gap    5702..5711
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5712..5971
                     /locus_tag="PKX82_05220"
     CDS             <5712..5971
                     /locus_tag="PKX82_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HOF51685.1"
                     /translation="HLAAAGCTTWFDYANYVLARVRIAYPAIQLIAKEVRAVPTSAFP
                     TAARRPHNSRLDTVKLRDTFGLHLPQWQDGVARMLSEIEIV"
     gene            5979..>6522
                     /locus_tag="PKX82_05225"
     CDS             5979..>6522
                     /locus_tag="PKX82_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOF51686.1"
                     /translation="MSPTTTRKGIILAGGSGTRLHPATLAISKQLLPVYDKPMIYYPL
                     STLLLAGIRDILIISTPQDTPRFAQLLGDGSQWGINLQYAVQPSPDGLAQAFIIGDSF
                     IGNSDSALVLGDNIFYGHDLHTLLANADQKTSGATVFAYHVQDPERYGVVAFDAAGKA
                     RSIEEKPVKPQSNYAVTGLYF"
BASE COUNT         1276 a         1895 c         2013 g         1328 t
ORIGIN      
        1 ggccgcgcgc tgatgagccg ccccaaggtg ctgctgctgg acgagccttc catgggtctg
       61 tcgcccatca tggtcgacaa gatctttgag gtgatcaagg atgtctatgc gcagggcgtg
      121 acggtgttgc tggtggagca gaacgccagc cgcgccctct ccattgcgga ccgtgggtat
      181 gtgatggagt ccggcattgt caccatgacc ggggatgcca agcacatgct caatgacccc
      241 aaggtccgcg ccgcatacct gggtgaatag gcacgtactt gcgctgctgt ttgtcaccct
      301 ggtgtggggg gcaacgtttc cggttctgaa gctcgccacg gcgcatctca gtggcgtgga
      361 aatatcggca ctgcgctttg tgattgcagc cctctgcatg gcaccctggg ccctgcgtgc
      421 atcgtgtcgc gcgtggacag atggcgctgt gctgggcgcg ctggttctgg tgtcctatgt
      481 ggcgcaagcc tgggggctgg agcatatttc ttccaatcgc agcgcgtttc tcaccagcct
      541 gaatgttctg atggtccctt tgctggggat cgtcctgggc aaccggctgt cctggacggt
      601 gctgctggcc gccgcaatgg cgtgtgtggg catcggtttg atgtcctggg acggtggtgc
      661 ccacctgctg gcggacagtg caactgtttt tggtgcattg gcgtatgccg gctatgtcat
      721 ctttttgtcg cgaaaggcag gattgcacag tgcccgtact ctggcggcca cccagatcgt
      781 ctggatggcc ttgctgggct ctgtctgggc atgggcagca tctttgggaa cagaccggat
      841 gcacaccttg cctgaacgta tcgacaccca gatactcttg gggttggcct acctgggtgt
      901 cgtggccact gcgggcatgc tgtttcttca ggccatggcg cagcggcatg tgtcggccga
      961 caaagccgcg ctgatctacg ccatggagcc tgtattcgct gccttgtttg cctggatatg
     1021 gcttgcggaa tccctctcct ggatggctgc tttcggggcg gtgattgtgg tgtctgcggt
     1081 cctgttgagt gagtggaagt caggcgcggg ctaacatcca ttcacattcg gtgtggccac
     1141 aaaaccggag gtgaccaggg cagcaacagg tcctgatcgg gcggatcagc cctgctgctt
     1201 gtctgcctcg gaagtgaacg cgtcggcgta gaactcttct tcgggcagac cggcagtggc
     1261 acagaaatcc ctgcgtgctg aatccaccac gatgggtgca ccacaggcat acacctggta
     1321 cccggagaga tccgggtggt cctgcagaac agcctggtgc acaaaacccg tgcggcccgc
     1381 ccacacatcc tgcggcaacg catcggagac gaccgggatg tactgcagcg taggcatggt
     1441 ttcacactgg gcttggaccc agctgtccat gtagagatcc gagggccgtc taccccccca
     1501 atacagagag attgcccggc tggaacccgt ctgctttagg tgctcgatca aggtcttgat
     1561 cggtgcgaac ccggtgccgg aagccagaag gatgataggt ttgtccgagt cttcacgcag
     1621 gaagaatgag ccatagggcc cctcaatgcg aaggatttcc ttttccttca tggcgccaaa
     1681 gacatggtcg gtgaacttgc cgccgggcat atggcggata tggagttcaa gaccagcacc
     1741 cgcttgtgga acactggcca tcgagtagct gcgccgggcg ccgtcgcgca aaaggatctc
     1801 gacgtattgg ccgggccggt aggtgaagtt gtcgttcgct ggaagttgga gctgcatcag
     1861 cacaacatcg tcggtcaggc gcgtcagggt gctgacccgg gtcgggagtt tcttgatggg
     1921 tatggctccg gcttctgtga cctggcgtga ttccagtacc agatccgatt gaggcgtcgc
     1981 gcagcatgtc agcacataac ctgcagcttc ttcctctgtg ctcaaggctt ttgactggtg
     2041 tgttccatgg gtcaccgatc cagaggtttt tttgcatttg caggagccac aggcgccatc
     2101 cttgcatccg tagggtaggc ctatgttttg gcgcaaacct gctgccagca ctgtttcatc
     2161 ggcctgggcg gagaattgcc gcccggatgg ctgcacggtc actgaaaaac tggagacaga
     2221 agtaacggtg gcagtcatct attgggtatc cttgggcaca aactatggac agcccccgat
     2281 tttgccttca atccagagcc ccctaggcgc tttgccatcc cgattccggc gtgaacgcgt
     2341 cctgatcgtc ggttgtggcg atgtggggca gcgtgttgcc ggcctgctgc gtcgcaacgt
     2401 gcgtgtggtc gcactgactt ccagcacggc ccgtgtctcg gaattgcgag cccagggagt
     2461 tgtgccgctg ctgggaaatc tggataaccc ggacagcctg cggcgtctgg cgggcatttc
     2521 cacacgcgtg gtgcacctgg ctccaccccc tgcgcatggc tggcaggacc tgcggaccca
     2581 ggcattgctg caggtgttgc gtctgcgcac gtcacctgct gcatgtgtat atgggtcgac
     2641 cagtggcgtg tacggagatt gcgcgggggc ctggatcgac gagacgcgta caaccaatcc
     2701 agactccccc agggcacgcc gcaggcagca cgcagaggat tgtgtgcgtt tcttcgggag
     2761 gtctgcagga atccgcaccc atacgctgcg cattcccggt atctacgccc cggatcggga
     2821 gggcgggacc cccagggccc gtctgctcaa gcgcacaccg gttttgtgcg cagaggaaga
     2881 cgtgtacacc aaccacatcc atgccgatga ccttgcacga gcctgtgtgc tggcaatgtg
     2941 gcgcgggcgg gcacaaaggg tgtacaacac caacgacgat acagcgctca agatggggga
     3001 ttacttcgac cttgccgccg atctgtatga cttgccacgc ccgccccggg tgtcacgcgc
     3061 aggggccaca gaacagttgc cattgatgct gttgagtttt atgggcgaat cccggcgcat
     3121 ggtgaaccgg cgtctgaagc aggagttggg gctgcgcttg cgctatccca cagttcggga
     3181 agggctggtt tccttggcgt aggcccagtg caatgccttc cataccagcc agctcgaagc
     3241 cagggtgatc gggtaggccc acagtgcgtc gctgaaccaa tgggccatgt cggacatgcg
     3301 tgcaaagccg atcacccagc ccgccagtgt tcccgtgacg gcccatgcca ccttgcgctt
     3361 acggtgaatc agcatcaggg acgtaaaaaa gaaaccacat gccacgtggc cgctgacaaa
     3421 agagcagttg gcgtcacact ggtttgtcat cacggcagcg cgggtaaatt gctgggtacc
     3481 accgaaattt tcaacctggt acgggcgtgc gcgctgccag tggtccttga aacccaggtt
     3541 caccacggcg cctggcccca gaatgccggc cagcaccaga aaagccatgg ccaagcgcag
     3601 cggcttgccc tgtttgcgga agcttgcaac caaccacacc accagcgcaa gcgcaatgcc
     3661 gacccggaat gcggccggta catacagatt gatcaactcc acccaccacc acccgatgga
     3721 agccactgct gggctgggtc cggcaaacag gctcgcagct tgcagatcca gtgtggtcca
     3781 tacggtcgga atcagggcca gcaagacgga aatgccccac acccagaagt acagggtggg
     3841 cgttttgcca aattgttccg gattggaggg cgtagcgggc atgaatgcag tgtggtggcg
     3901 gtcaaaccac cgattgtgac gcaggcttat gctaaccttt gcatccatac caacaagctc
     3961 cctgcaggag cgcaacacag aactgccatg actattcttg tcaccggggg agccggtttt
     4021 attgggtcga atttcgttct ggactggctg tcacagtccc aggagcctgt cgtcaatctg
     4081 gacaagctga cctacgctgg caatctggaa aatctggcca gtctggatgg ggatgcgcgc
     4141 catatttttg tgcgcgggga tatcggcgat tcggccctgg tttccggttt gctggcccag
     4201 caccagccgc gcgcgattgt caatttcgct gcggagtcgc atgtggaccg gtccatccac
     4261 gggccagaag actttattca gaccaatatc ctgggcactt tccacatgct ggagtgcgtg
     4321 cgtgcctatt ggggtgcctt accgactgaa gccaagggcg cgtttcgatt ccttcatgtg
     4381 tccaccgacg aggtctatgg ctcactgtcg aaaaccgacc cagcctttac cgaagaaaac
     4441 aaatacgaac ctaacagccc ctactcggcc agcaaggccg ccagcgacca cttggtgcgt
     4501 gcctaccacc acacctatgg cctgccggtg ctcaccacca actgcagcaa caactacggc
     4561 ccgttccatt ttcctgaaaa actgatcccg ctgatgatcg tcaatgccca ggcgggcaag
     4621 cctctgccgg tgtacggtga cggccagcag atccgcgact ggctgtatgt caaggaccat
     4681 tgcagcgcca ttcgccgcgt gctggacgcc gggcaactgg gcgagacgta caacgtgggt
     4741 ggctggaacg aaaagccgaa tctggacatc gtgcacactg tgtgtgcact gctggacgaa
     4801 ctcaagcccc gcgccgatgg caagccttac caaaagcaga tcacgtatgt gacggaccgg
     4861 cccggccatg accgccgata cgccattgac gcgcgcaaga tcgagcaaca gttgggctgg
     4921 aagcccgcgg agaccttcga gaccggtatc cgcaagacgg tgcagtggta cctggaccat
     4981 ccgcagtggg tggccaatgt gctcagcggc ggctaccgtg actgggtcga aaagcaatac
     5041 ggcgacagtg ccaagagcgt ggcatgaaga tactgctgtt gggccgcgac ggccaggttg
     5101 gctgggagct acagcgcagc ctggcgcctt tgggtgaagt tgttgcgttg gggcgcgata
     5161 cagcagccaa tccgcagggg ttgtgcggtg acttgacaca gctggaggat ctggctgcga
     5221 cggtgcgcgc ggtcgcgcca aacgtcatcg tcaatgcagc ggctcacacg gcggtcgaca
     5281 aggcagagag tgaagtggac ctggcacggg ttatcaacgc agtcgcgccc ggcgtgctgg
     5341 ccgacgaggc gcagcgcctg ggcgcgtggc tggtgcacta cagcacggac tatgtatttg
     5401 acggcagtgg tgaggcacct tggaaagaaa cggatggcac cggaccgctc agcgtgtacg
     5461 ggcgcaccaa acttgcgggc gagcaggcgg tcgcacgctg cgacaaacat ctggtgtttc
     5521 gcaccagctg ggtatattcc gcacgtggcg gcaactttgc cagaaccatg gtgcggctgg
     5581 cccaaacgcg agagttgctg caggtcatca acgaccagta tggcgcgccc accggtgcgg
     5641 acctgctggc cgatgtgacg gcccatgcca tccgccaggt ggcgtaccac ggtgcaacgg
     5701 gnnnnnnnnn naccatctgg cggcagcggg ttgcactacc tggtttgact atgcaaacta
     5761 tgtcctggcc cgcgtacgta ttgcgtaccc agctattcaa ttgatagcaa aagaggttcg
     5821 cgctgtgcct accagtgcat ttcccaccgc cgccagaagg ccgcacaact cgcgtctgga
     5881 tacggtcaaa ctgcgcgaca cgtttggcct gcatctgccg caatggcagg atggcgttgc
     5941 ccgcatgctt tcggagattg aaattgtcta aaaaatccct gtcacccacg accacccgca
     6001 agggcatcat ccttgccgga ggctccggca cccgtctgca cccggcgacg ctggctatca
     6061 gcaagcaatt gctgccggtc tatgacaagc ccatgattta ctacccgctg agtaccttgc
     6121 tgcttgcagg tattcgggat atcctgatca tcagtacccc gcaagacacc ccacggtttg
     6181 cgcaactgct gggtgacgga agccagtggg gtatcaacct gcaatacgcc gtgcagccca
     6241 gtcccgacgg gttggcgcag gccttcatca ttggcgacag ttttatcggc aacagcgaca
     6301 gtgcgctggt gctgggcgac aacatttttt acggtcacga cctgcatacg ctgctggcca
     6361 atgcggatca aaaaaccagc ggtgcgaccg tatttgccta ccatgtgcaa gaccccgaac
     6421 gttacggtgt ggtggcgttt gatgccgctg gcaaagcgcg cagcatcgag gaaaagccgg
     6481 tcaagccgca gagcaattac gccgtcaccg gcctgtattt tt
//
LOCUS       DAOSJM010000107         6513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10762_length_6513_cov_3.901363, whole genome shotgun sequence.
ACCESSION   DAOSJM010000107 DAOSJM010000000
VERSION     DAOSJM010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10762_length_6513_cov_3.901363"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            548..1198
                     /locus_tag="PKX82_05230"
     CDS             548..1198
                     /locus_tag="PKX82_05230"
                     /inference="COORDINATES: protein motif:HMM:NF039641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HOF51687.1"
                     /translation="MAIPFTIDQFYGVFRDYNTAVWPVQWILAALGFAEVVAVLRPQP
                     WTGIAVSAGLGFLWAWIALAYHLVFFTSISPAAYGFAALSAAAAVLFIWQGVVRRRLD
                     FRWLPGLKGTVGIMLIAFALVVYPVWSAYAGHAYPETPTFGLPCPTTIFTIGLLCFAK
                     PPTPRSPLIVPLLWCFVGAQAALFLGVQPDLGLIAAGVVGAALLASARRPLRTLKL"
     gene            1195..2304
                     /locus_tag="PKX82_05235"
     CDS             1195..2304
                     /locus_tag="PKX82_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOF51688.1"
                     /translation="MITRRQFAASTPLLAASALALASCSSEPASDDYEAVAERTWHPG
                     TATDLSGAALGRELVRFATLAPSSHNTQCWKFALDGSGRSITIQPDLARRCPVVDPDD
                     HHIFVSLGCAAENLVQAALAHGHRGEAQFDAAGNAVRVVLKPTRAQTTPLFTAIPLRQ
                     CTRGDYDGKSLSSEELALLQRAGSSDAVRLLLLTERPAMEQTLDYVMQGNTAQLADAA
                     FVKELKDWIRFNGSDAVRTRDGLYSVSSGNPSIPSWIGDLVFRWLLTAKGENDKAARQ
                     VRSSAGIAVFVGQAADKANWVEVGRCYERFALQATAMGIRNAFLNQPVEVAALRPPFA
                     AALGLSGQRPDLVVRFGRGPVLPRSLRRPVEAVLV"
     gene            2333..2593
                     /locus_tag="PKX82_05240"
     CDS             2333..2593
                     /locus_tag="PKX82_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51689.1"
                     /translation="MTLLLWLGIVLGALVVLEIGLRAAGAARWAEMTRTHTTQLESGS
                     IDAHELEGLPPPVQRYFRAVLTDGHRCHRGLARRCGELGQHR"
     gene            2714..3646
                     /locus_tag="PKX82_05245"
     CDS             2714..3646
                     /locus_tag="PKX82_05245"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOF51690.1"
                     /translation="MKLTLRRSMPVLLALLLVGAAVLIGWRFNHDLALATARAAQGGV
                     LLPTRCGLVEYQQAGTGVPLLVVHGSGGGHDQGMAFAGTLARHGVRVIAMSRFGYLGT
                     PMPSDASVAAQADAHVCLLDALGIGRAAVMGGSAGAPSALQMAIRHPDRVRALILLVP
                     LAYKPAARADSAPPMSAWIEATMMRLIGSDFLFWAALHVTRDQAIKVVLATPPALLKT
                     ASPSEQARVNAMLENILPVSSRAKGLISDTATGKHLAPAPLESIRAPTLVISARDDRY
                     GTYAGRSTPPVKSPGRSSSASIKAVIPGLDMTPR"
     gene            complement(3792..4001)
                     /locus_tag="PKX82_05250"
     CDS             complement(3792..4001)
                     /locus_tag="PKX82_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51691.1"
                     /translation="MFRTAKTEKYFPTVALRIKRWASYKTRTSAGGSAISAARPAMFR
                     FSIASLHCSDGMVYFHFEVDWDAQA"
     gene            complement(4034..5155)
                     /locus_tag="PKX82_05255"
     CDS             complement(4034..5155)
                     /locus_tag="PKX82_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOF51692.1"
                     /translation="MPKALTVEVAAVTQGRFERAVQDYGKTRVRDRYTVSAPLAGRVG
                     RVMLSQGDSVAQGDTVALLWPMAPALIDERTRAEQAARIGAMASAMARSQANVGRAKA
                     AMEQAQTELKRSELLAQQGFVSPNQNEAGRLNLRLRMQELESARQEENAARYELEQAQ
                     VARRQFAQGPLGGAQPSFAVKAPVSGKVLKVLQQSEASVLPGAGLVELGDPTKLDVVV
                     DILTEDAIEVQPGAAVELLNWGGPQALAGKVRWVEPAAFTKVSALGVEEQRVNVVIDI
                     SAPEAQLRALGDGFKVDVRVLVQVVEGAVLVPVSALFPIGSRSGIFVVDKDHATLKEV
                     SVKARNGVSAWVKEGLAVGTQVIVYPDSKLKNDAKVKVR"
     gene            complement(5401..6384)
                     /locus_tag="PKX82_05260"
     CDS             complement(5401..6384)
                     /locus_tag="PKX82_05260"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF51693.1"
                     /translation="METQFRQVLRGDVMGVLLEATPARVLYALGKLPHVTAVEEQRSV
                     PVRLVNAHLSWRGTLIGKPQHPELSRIMDMDFRAHSAPQSGLLLTDRLAEKLQLQVGD
                     MVRVELQEGRREVLTLPISGLVHEMLGMNAYLERRTLNALLREGDMVNSFALAVERGH
                     EPALLNRLKDLPRVGMVISKSTMARNIEDVTVGNLLMFSTVLTAFAVVIAVGVVYNHA
                     RIALAERAWELASLRVLGFTRSEVSAILLGELAIEIVLAMPLGMVMGYLLSKGIVSVI
                     QTDEFFFPFVIAAATYAFSAVCIIVAGVVSAFIVRRRVDALDLVSVLKSRE"
BASE COUNT         1136 a         2113 c         2003 g         1261 t
ORIGIN      
        1 agtcagctta cgggttgtcc aatccatcgc ccgttagaag ccgatcatgg cggacttcaa
       61 cgtgcccata acggtcagcc acggtttgag gaagcagcca ttcaaactgg tgcacgaaaa
      121 cctttaagcc gacatttgta gacgctgagc aattcccttc ggctgtgcgg ctcttgtgca
      181 gcgtaaatgg ttatacagaa attcaaatta cccgccgaac acctgaaatt ggcttttgga
      241 gcccgaatgt tacttgcggg gcagcgagtg caaccaaatg gacacccgga ctcgaccatg
      301 agccgttgct caccaccgac cgctgtcaaa cagcccaatc aacttctatt tcgactgtcg
      361 cagcagcgtt ctactcggcg atcgcttggc tcttgctcgc gacctttggc ttcggaagcc
      421 agactcgtgc aggcggacag cgcgcttgtt gctgaggcca ggagttcgaa cgactccgcc
      481 cggagccgtc acgttgagtt cggtagtgca cagactgacg ctgcctttgg cactatggtg
      541 gtgaaatatg gcgatcccat tcacgatcga tcagttttac ggtgtcttcc gtgactacaa
      601 cacggccgtg tggccagtgc agtggatcct tgcggccctt ggtttcgccg aggtggttgc
      661 ggttctgagg ccgcagccat ggaccggcat cgccgtgtcc gctggcctcg ggttcctttg
      721 ggcttggatc gcattggcct atcaccttgt cttcttcaca agtatcagcc ctgcggccta
      781 tggctttgcg gccctctccg ccgccgctgc ggtcttgttc atctggcagg gggtggttcg
      841 gcgcaggctc gacttcaggt ggttgccagg cctgaagggc acagtcggca tcatgctcat
      901 cgccttcgcc ctggtggtgt atccggtctg gtcggcctac gccggtcatg cgtatccgga
      961 gacgccaacg ttcggcctgc cgtgcccgac aacaatcttc accatcggcc tgctgtgctt
     1021 tgcaaagccg cccacaccgc gcagcccgtt gatcgttcct ttgctgtggt gctttgtcgg
     1081 tgctcaagct gcactcttcc tgggcgtgca gcccgacctc ggtctcattg ctgcaggcgt
     1141 ggtgggcgca gcactgctcg cgtccgcgcg ccgaccactt cggacactga aattatgatc
     1201 acacgcaggc agttcgccgc ttcgacgcca ttgctggcgg ccagcgcttt ggccttggcc
     1261 agttgctcct cggagcctgc atccgatgac tacgaagctg tcgcagagcg cacttggcac
     1321 ccgggtacgg cgaccgatct tagcggcgcg gcgctcgggc gggagctggt tcgctttgcc
     1381 accctcgccc cttccagcca caacacacag tgctggaagt tcgcgctcga cggctcaggc
     1441 cggtcgatca ccatccagcc tgacttggcg cgccgctgcc ctgtggtgga cccggacgac
     1501 catcacatct ttgtttcgct gggctgtgcc gccgagaacc tggtccaggc cgcgctcgcc
     1561 cacggccaca ggggcgaagc gcagttcgac gcagccggca acgcggtgcg cgtggtgctc
     1621 aagcccaccc gagcgcagac cacgcccttg ttcacagcca tcccgctgcg ccaatgcacc
     1681 cgcggtgact acgacggcaa atctttgtcc agcgaagaac tagcgctgct gcagcgtgcc
     1741 ggaagttcgg acgccgtgcg cctgctgctg ctgacggagc gcccggcgat ggagcagacg
     1801 ctggactacg tgatgcaagg caacacggcg cagttggccg acgcggcctt cgtcaaggag
     1861 ttgaaggact ggatccgctt caacggcagc gacgccgttc gcacgcgcga cgggctctac
     1921 agcgtgtctt ccggcaaccc gagcatcccg tcatggattg gcgacctggt gtttcgctgg
     1981 ctcctgaccg cgaagggcga aaacgacaag gctgcgcggc aggtgcgcag ttccgccggc
     2041 atcgctgtct ttgttggcca ggccgccgac aaggcgaact gggtggaagt ggggcgttgc
     2101 tatgagcgtt ttgcgctgca ggccacggcc atgggcatcc gcaatgcctt cctgaaccag
     2161 ccggtcgaag tggcggcgtt gcgaccaccc ttcgctgccg cactgggctt gagcggccag
     2221 cggccggacc tcgtcgtgcg gttcgggcgt ggcccggtgc tgccgcgttc gctgcggcgg
     2281 ccggtcgagg ccgtcctggt gtgagcgaca tattcgaacc aaaggaactg cgatgacgtt
     2341 gctgctgtgg ctggggatcg ttctgggtgc gctggtggtc cttgagatcg gcttgcgcgc
     2401 agccggcgcc gcccgatggg ccgagatgac ccgcacgcac accacccagc tcgaatccgg
     2461 cagcatcgac gcgcacgaac ttgagggctt gccgccgcca gtgcagcgct acttccgcgc
     2521 ggtgctgacc gacgggcacc gttgccacag gggcctggct cggcgctgcg gggaactcgg
     2581 gcaacaccgg tgagccgcat ctgcatgtcc atgcgcagcg gccgggtcct gtgggtgcgc
     2641 cattgagcgg cgaacccttg ccgatccttt tcaacggcca ctttcccgtg cgcggcgaca
     2701 gcattgattt gccatgaagc tgaccctgcg acggtccatg cccgtcttgc tggcgcttct
     2761 gctggtgggc gcggcggttc tgattggctg gcgcttcaac cacgatctgg cccttgccac
     2821 ggcgcgcgcg gcacagggcg gggtgctgct gcccacgcgc tgcggtctgg tcgaatacca
     2881 gcaagcaggt acaggcgtcc cgctgctcgt cgtgcacggc agcggcggcg ggcatgatca
     2941 gggtatggcg ttcgcgggga cgctggcacg acacggtgtc cgtgtgatcg cgatgtcgcg
     3001 cttcggatac ctcggcacgc cgatgccgtc tgacgcgtcg gtggccgcgc aggccgatgc
     3061 gcacgtatgc ctgctcgatg cgctcgggat tggccgtgcc gcggtcatgg gtgggtcggc
     3121 aggcgcgccg tcggcgttgc agatggcaat ccgccacccc gatcgtgtcc gcgcgctgat
     3181 cctgctggtg ccgctggcct acaaacccgc cgcccgggcc gattcggcac cgccgatgtc
     3241 ggcatggatc gaggccacga tgatgcggct gatcggctcg gacttcctct tctgggcggc
     3301 gcttcatgtc acccgtgatc aggcgatcaa ggtcgtgctg gccaccccac ctgcgttgtt
     3361 gaaaacggcc agccccagcg agcaggcccg cgtgaatgcg atgttggaga acatcctgcc
     3421 ggtctccagt cgcgccaagg gtttgatcag cgacacggcc acaggcaaac atttggcacc
     3481 agcaccgctg gagtccatcc gcgcacctac gctcgtcatc agtgcgcgtg atgatcgcta
     3541 cggcacctac gccgggcgca gtacaccgcc agtcaaatcc ccggggcgga gttcgtcggc
     3601 ttcgatcaag gcggtcatac ctgggttgga catgactccg aggtgatggc cgagatcgtg
     3661 cgcctattgg tcccggcgga ccgcccatga cgctcaccgc gggggtgcga agttacccac
     3721 ttggataccg gcctcatttt tgtggcggtg gccatggcgc gttgccgcac agacgccatt
     3781 tgacagtgtc gttaggcttg cgcatcccaa tccacctcga aatgaaagta caccatgccg
     3841 tcactgcagt gcaaggaagc gatcgagaaa cgaaacattg ccggtcttgc agcggagatt
     3901 gcgctgccgc cggcggaggt gcgcgtcttg tacgaagccc agcgcttgat gcggagtgcc
     3961 acggtgggga agtacttctc cgtcttcgcg gtgcgaaaca ttcgacggca attgaaacac
     4021 ccggtctgaa gcctcaccgc accttgacct tcgcgtcgtt cttgagcttg ctgtccgggt
     4081 aaacaatcac ctgggtgccc accgccaaac cttctttcac ccaggcgctc acgccattgc
     4141 gggctttcac gctgacttcc ttcagagtgg cgtggtcttt gtccaccaca aagatgccgg
     4201 agcgcgagcc gatgggaaac agcgcgctga ccggcaccaa gaccgcgccc tccaccacct
     4261 gcaccagcac ccgcacgtcc accttgaatc catcgcccag cgctctcagc tgcgcttcgg
     4321 gtgcgctgat gtctatcacc acgttgacgc gctgctcttc cacgcccaga gccgacacct
     4381 tggtaaacgc agcgggttcc acccagcgga ccttgcctgc gagcgcctga gggccgcccc
     4441 agttcagcag ttccaccgcc gctccgggct gcacctcgat cgcatcttcg gtcaggatat
     4501 ccaccaccac atccagcttt gttggatcac ccagctccac gagtcccgcg ccgggcaaca
     4561 cactggcctc gctttgctgc aagaccttga gcactttgcc gcttaccggt gccttgacgg
     4621 caaagctggg ctgtgcgcca cccagcggcc cctgcgcaaa ctgccgacga gccacttgag
     4681 cctgttccag ttcgtagcgt gcggcgtttt cttcctggcg tgcactttcc aattcctgca
     4741 tccgcaggcg caaattgagg cgtcccgctt cattctggtt gggcgaaacg aacccctgct
     4801 gggccagcaa ttcactgcgc ttcaactctg tctgcgcctg ctccatggcg gctttggcac
     4861 gccccacatt ggcctgagaa cgggccattg cagaagccat agcgcctatg cgcgctgctt
     4921 gttcagcccg ggtgcgctcg tcgatcagcg ccggtgccat gggccacagc agggctacgg
     4981 tatccccctg tgcgacgctg tcaccctgac tcaacatcac ccggccgaca cggcctgcca
     5041 ggggtgcaga tacggtgtac cggtcccgca cccgcgtctt gccgtaatcc tgaacggcac
     5101 gctcgaaccg gccctgtgtc acggcagcta cttccactgt gagtgctttg ggcatgaatg
     5161 cccataccag cagacccatg acagcaaggc ccactccacc caagatcaga ttgcgtttca
     5221 agctggactt ggccttgggc gcggttgctg gagcgccgga tgcaggtgtg gccgatgctg
     5281 gtgccttacc cggatttgaa ggcggcggcg cagctttcgg cgcaggtgtt gcagggactg
     5341 gggcaacggt ggggtcggtg ctagtgttca tttggaatcc aggtgatggc ggtgggcaat
     5401 tcattcgcgc gacttcagta cgctgaccag gtccaatgca tccacacgcc gacgaactat
     5461 gaaggcgctg acgacgccgg ccacgatgat gcagacggcg gaaaatgcat aggtggcggc
     5521 ggcgatcacg aagggaaaga agaactcgtc tgtctgaatc acgctgacga tgcctttgga
     5581 cagcaggtag cccatcacca tgcccagcgg catggccagc acgatttcaa tggccagctc
     5641 gccgagcaat atggcagaaa cttccgagcg ggtaaagccc agcacacgca agcttgccag
     5701 ttcccaggca cgctcggcca gagcaatgcg ggcgtggttg tagaccacac cgacggcgat
     5761 cacgacggca aatgcggtca gaaccgtact gaacatcagc aggttgccaa cggtcacgtc
     5821 ctcaatgttc ctggccatgg tggacttgct gattaccatg ccgacgcggg gcaaatcctt
     5881 gagccggttg agcaatgcgg gctcatgacc gcgctccacg gccaaggcaa agctgttcac
     5941 catgtcaccc tcgcgcagca gcgcgttcag ggtgcggcgc tccaagtatg cgttcatgcc
     6001 cagcatctcg tgcacgagcc cactgatggg gagcgtcaac acctcgcggc ggccctcctg
     6061 cagctccacg cgtaccatgt cgcccacttg gagctgcagc ttttccgcca atcggtcagt
     6121 cagcaacagg ccgctctgcg gggcgctatg ggcccggaaa tccatgtcca taatgcggct
     6181 cagttcggga tgttgtggct tgccaatcag ggtgccacgc cagctgagat gggcattgac
     6241 aaggcggacc ggcacactgc gctgctcctc cacggcggtc acgtgaggca gcttgcccaa
     6301 ggcatataac acccgggcgg gcgtggcctc tagcaagaca cccatgacat cgccgcgcaa
     6361 gacctgccgg aactgcgttt ccatgagtac tgccaccgaa tcgcgcatga aggagccaga
     6421 tcacaattgc catggacacg gccacgccaa acaccgtcag actggtgcgc agtgggcggc
     6481 gcgccatggt gcgcagcacc atacgcagcg cag
//
LOCUS       DAOSJM010000108         6421 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10940_length_6421_cov_5.107603, whole genome shotgun sequence.
ACCESSION   DAOSJM010000108 DAOSJM010000000
VERSION     DAOSJM010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10940_length_6421_cov_5.107603"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKX82_05265"
     CDS             <1..408
                     /locus_tag="PKX82_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOF51694.1"
                     /translation="SLMVIEHAERFGLSQLHQLRGRVGRGAAASACVLLYSTGDAPRL
                     GEAARERLKAMVETNDGFEIARRDLEIRGPGEFLGARQSGEAMLRFADLATDTVLLEW
                     ARTEAPMMLDQHPHLAEKHVQRWLGGKAEFLKA"
     gene            509..1501
                     /locus_tag="PKX82_05270"
     CDS             509..1501
                     /locus_tag="PKX82_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51695.1"
                     /translation="MLTHPFEFNLAWLVAGLAVASVVAWFGWIEYQAHLLKTRVEQAG
                     GVLRFVAHGWSVEAQRSTNEVVVHSRHAHYSRQPAGGGSEQVQDGHVTAKLPAPGLRI
                     DIKPGHSAGQCVVEFHASDELTLAAQGKAGGESAVVRLDRVPEPVAASFGQFASQLRL
                     WVEKLEKRLTIQREAEVAEKAAQEAAQAKAAAAAAAAATGEASTEELTPDVQVARWRK
                     AAGFAGTSSEIGLNDKGGILWYIDLDPTGRITLHANNRTIHTTLAGASIASLGGELEV
                     GVRDDYWSEDEPELRTFRILKGLPPDERRAWKERMEILRDNLRQAAGIPAQTGR"
     gene            1517..2476
                     /locus_tag="PKX82_05275"
     CDS             1517..2476
                     /locus_tag="PKX82_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOF51696.1"
                     /translation="MTLTELKYIVAVARERHFGKAAEACFVSQPTLSVAVKKLEEELD
                     VKLFERSANEVAVTPLGEEIVRQAQSVLEQAANIKDIAKRGKDPLAGPLKLGVIYTIA
                     PYLLPDLVRQVITHSPQMPLMLQENFTVKLLEMLRTGEIDCAILAEPFPDTGLAVAPL
                     YDEPFYAAIPMTHPLAKKDSVTTEELKSETMLLLGNGHCFRDHVLEVCPEFARFSSDA
                     EGIRKSFEGSSLETIKYMVAAGMGITLVPRLSVPREALEDRKRRHEDIYVKYLPVQDG
                     KGALPPTRRVVLAWRRSFTRYEAIAALRNAIYGCELPGVTRLS"
     gene            2546..3397
                     /gene="ubiA"
                     /locus_tag="PKX82_05280"
     CDS             2546..3397
                     /gene="ubiA"
                     /locus_tag="PKX82_05280"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOF51697.1"
                     /translation="MTPRFSLYLDLIRWNRPAGWLLLLWPSLSALWLAAGGFPGWHLV
                     IVFTLGTILMRSAGCCINDVADRDFDRHVKRTAQRPVTSGAVSVREALVVGAVLALAA
                     FGLVLTTNAHAVAWSFAALAVSLAYPYAKRFVSMPQAVLGVAFSFGIPMAYAAVQADI
                     PLQAWVLLVGNLFWVLAYDTEYAMVDRDDDLRIGMKTSAITLGRWDVPAVLAFYVIHL
                     AIWSWVIAPLVHGWGWWLGVAAALAQVVWHATLIHDRTRDGCFKAFRLNHWVGFSLFC
                     GVALSFV"
     gene            3485..3766
                     /locus_tag="PKX82_05285"
     CDS             3485..3766
                     /locus_tag="PKX82_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HOF51698.1"
                     /translation="MRTSPCSAAIDSAALCESSISRIVEMAWEDRTPFEAIEAQFGLN
                     ESAVMELMRRHMKPSSFRMWRKRMAGRRTKHAVLRGAVFLRHRATHTRQ"
     gene            3779..5332
                     /locus_tag="PKX82_05290"
     CDS             3779..5332
                     /locus_tag="PKX82_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase/cryptochrome
                     family protein"
                     /protein_id="HOF51699.1"
                     /translation="MTTRRALVWFKRDLRVHDHAPLVAALADADALALFVIEPEWMRS
                     PECDGSHVDFALACLAELRAALALRGLPLLVRVGSAVEVLAQLHSEVAFTHLLSHEET
                     GPGWSYVRDKQVAAWCQSVHVPWQEFTQTGVVRRLRTRSGWAQRWQARMDAPLHLLDG
                     RFSAAVTLDQPDLPTLSSLGVAPHGKVLQAAGERAARHTLQSFLQVRGHDYRKALSSP
                     LSAEEGCSRLSPHLAFGTLSLRTVHQATEVAIGSTPDRTVAYALRGFAGRLRWHCHFM
                     QKLEDEPDIEFHNFARVYDGLRENDFNDDHFAAWCEGRTGYPMVDACMRSLLATGWLN
                     FRMRAMLVSFASYHLWLHWRPTGLFLGRQFLDFEPGIHWSQMQMQSGTTGINTLRIYS
                     PTKQALDQDPQGGFIRRWVPELSKVPLPYLAEPWKMNVSVQRMAGCMIGVDYPAPIVD
                     DKLAMKTAKDRMYGLRKTQEAREEAGDVQARHGSRKSGLPPSGQRAKAASRGAKGPAA
                     PSPQGDLFA"
     gene            5329..5493
                     /locus_tag="PKX82_05295"
     CDS             5329..5493
                     /locus_tag="PKX82_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HOF51700.1"
                     /translation="MKNDFKGNKQSLPSKLCAVCGRTMTWRKAWAKNWESVLYCSDAC
                     RARKTNKKAP"
     gene            5490..>6421
                     /locus_tag="PKX82_05300"
     CDS             5490..>6421
                     /locus_tag="PKX82_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HOF51701.1"
                     /translation="MTPTDTHPATALRLAPLPATVRNLVIVLGDQLDAESSALQDFDP
                     TQDVVWMAEVAQESTHVWSAKQRIAVFLSAMRHFADDLRRQGLPLVYARLDDADTRGT
                     LALELDKAITQLHPATLVITAPGDWRVLHSLRAVAAEHQLPLDLRDDPHFFSTVRDFA
                     AHAQGRKQLRLEYWYRELRQKHGILMDGKEPAGGQWNFDADNRESFGKAGPQNVPAPT
                     RFAPDATTQDVIALVHTRFASHPGSLGSFGWPVTRTQALAALQAFIQERLPLFGLYED
                     AMWSGEPWLYHSHLSCALNLKLLNPREVVQAAEDA"
BASE COUNT         1139 a         1991 c         2093 g         1198 t
ORIGIN      
        1 tcgctgatgg tgattgagca tgccgagcgt tttggcctga gccagttgca ccagctgcgt
       61 ggccgggtgg ggcgcggcgc ggcggcgtcg gcctgcgtgc tgctgtattc cactggagat
      121 gcgccgcgtc tgggtgaggc cgcgcgcgag cggctcaagg ccatggtgga aaccaacgac
      181 ggcttcgaga tcgcccggcg cgacctggag attcgcggcc ccggtgagtt tctgggcgcc
      241 cgccagtcgg gtgaagccat gctgcgcttt gccgatctgg cgacggacac cgtcttgctg
      301 gagtgggccc gcacggaagc cccgatgatg ctggaccagc atccgcacct ggcggaaaag
      361 catgtacaac gctggctggg cggcaaagcc gagtttctga aggcctgacg ccgcatattt
      421 tgatgagaat gacctccagc ccccgaatat attgcgcaag tagctataaa aacagtagca
      481 aacgtatgcc cgtacaagga ttgaccccgt gctgacccat ccctttgaat tcaacctggc
      541 ctggctggtg gccggcctgg ccgtcgcatc ggtggtggcc tggtttggct ggatcgagta
      601 ccaggcccat ttgctcaaga cccgcgtgga gcaggccggt ggcgtgttgc gttttgtggc
      661 gcacggctgg tcggtggagg cacagcgcag cacgaacgaa gtggtggtgc attcccgcca
      721 tgcgcattac agccgccagc cggctggcgg cggttccgag caagtgcagg acgggcatgt
      781 cacagccaaa ctccccgcgc cgggattgcg tatcgacatc aagcccggcc attcggccgg
      841 gcagtgtgtg gtcgagtttc atgcctccga cgagctcact ctggcagcgc aaggcaaagc
      901 cgggggcgag agcgctgtgg tgcgcctgga tcgcgtcccg gaaccggtgg cggccagctt
      961 tgggcagttc gcgtcgcagc tgcggctgtg ggtggaaaaa ctggaaaagc gcctgaccat
     1021 ccagcgcgag gccgaggtgg ctgaaaaagc tgcccaggaa gctgctcagg ccaaagccgc
     1081 agcggctgca gcagcggcgg cgaccggcga agcttccaca gaggagttga cccccgatgt
     1141 gcaggtggcc cggtggcgca aggcggccgg gtttgccgga acttccagcg agattggcct
     1201 gaatgacaaa ggcggcattc tctggtacat cgacctggac cccaccggcc gcatcacgct
     1261 gcacgccaac aaccggacca tccacaccac gctggcgggg gccagcatcg catccctcgg
     1321 cggtgagctg gaggtgggtg tgcgcgatga ctactggagc gaagacgagc ccgagttgcg
     1381 caccttccgc attcttaaag gcttgccgcc cgacgagcgc cgcgcctgga aggaacgcat
     1441 ggagatcctg cgcgacaatt tgcgccaggc cgccggcatc ccggcacaga cagggcggta
     1501 ggaggacaat agcgccatga ctctgaccga actcaaatac atcgttgccg tggcgcgcga
     1561 gcggcatttt ggcaaggcgg ccgaagcctg ctttgtgtca cagcccacgc tgtcggtggc
     1621 ggtgaagaag ctggaagaag agctcgacgt caagctgttc gagcgcagcg ccaatgaggt
     1681 ggcggtgact ccgctgggcg aagagatcgt gcgccaggcg caaagcgtgc tggagcaggc
     1741 tgccaacatc aaggacattg ccaagcgcgg caaggatccg ctggccggcc ccctcaagct
     1801 gggggtgatc tacaccatcg cgccctacct gctgcccgat ctggtgcgcc aggtgattac
     1861 ccattcgccg cagatgccgc tgatgctgca ggagaacttc acggtcaaac tgctggagat
     1921 gttgcgcacc ggtgagatcg attgcgccat cctggccgag ccttttccgg acaccggact
     1981 ggcggtcgcg ccgctgtatg acgagccttt ttacgcggcc atccccatga cgcatccact
     2041 ggccaaaaaa gacagcgtca ccaccgaaga actcaagagc gaaaccatgc tgctgctggg
     2101 caatggccac tgctttcgcg accatgtact ggaagtctgc ccggagttcg cgcgcttttc
     2161 cagcgatgca gaaggcatcc gcaagagctt tgaggggtcg tcgctggaga ccatcaagta
     2221 catggtggcc gcgggcatgg gcatcaccct ggtgccgcgc ctgagcgtgc cgcgcgaagc
     2281 gctggaagac cgcaagcgcc gccacgaaga catctatgtc aaatacctgc cggtgcagga
     2341 cggcaagggc gcactgccgc ccacgcgccg cgtcgtgctg gcctggcggc gcagctttac
     2401 ccgttacgaa gccatcgcgg ccttgcgcaa cgccatttat ggctgtgagc tgcctggagt
     2461 gacccggctc tcgtaatttt gaacaaaatt ggctgctggc ccccgtattt attgcgtagg
     2521 cagctatcaa aataatagca tttttgtgac accacgtttt tcgctgtacc tggacctgat
     2581 ccgctggaac cggcctgctg gctggctatt gttgttgtgg ccctcgctga gtgcgctgtg
     2641 gctggcggcg ggcggctttc cgggctggca cctggtcatt gtctttaccc tgggcaccat
     2701 cctgatgcgt agcgcaggct gctgtatcaa cgatgtggcc gaccgtgatt tcgaccgcca
     2761 cgtcaagcgc accgcgcagc gcccggtcac cagcggtgcg gtgtcggtgc gcgaggcgct
     2821 ggtggtcggt gccgtgctgg cgctggcggc gtttggcctg gtgctcacca ccaacgcgca
     2881 tgccgtggcg tggtcatttg ctgcactggc ggtgtcgctg gcctacccgt acgccaagcg
     2941 gtttgtttcc atgccgcagg cggtgctggg cgtggcgttc agctttggca tccccatggc
     3001 ctatgccgca gtgcaggccg atattccgct gcaggcctgg gtgctgctgg tgggcaacct
     3061 gttctgggtg ctggcctacg acaccgagta cgccatggtg gaccgcgatg acgacctgcg
     3121 catcggcatg aagacctcgg ccatcacgct ggggcgttgg gatgtgccgg cggtgctggc
     3181 gttctacgtc atccatctgg ccatctggtc ctgggtgatt gccccgttgg tgcacggttg
     3241 gggctggtgg ctgggcgtgg ccgccgcgct ggcgcaggtg gtgtggcacg ccaccctgat
     3301 ccatgaccgc acccgcgacg gctgcttcaa agccttccgg ttgaatcact gggtggggtt
     3361 ttcgctgttc tgtggggtag ctttgtcgtt cgtgtgagcc ggcggcaaaa ataccctgcc
     3421 ggggtgctgc gtcttaggta attcggcaaa attccgcctt ggtttcactg tgtccaaggt
     3481 gcctatgcga acttctccct gcagcgctgc gattgactcc gcggcacttt gcgaatccag
     3541 catctcccgc attgtggaga tggcctggga agaccgcaca ccgttcgagg ccatcgaggc
     3601 ccagtttggt ttgaatgaat ctgccgtgat ggagttgatg cgacggcata tgaagccttc
     3661 gtctttccgg atgtggcgca agcgcatggc ggggcgccgc accaagcatg cggtgctgcg
     3721 cggtgccgtt ttcctgcgcc accgcgccac ccacacgcgg caatagcagg tcgccacgtt
     3781 gacaacacgc cgagctctgg tctggttcaa gcgcgatctg cgggtgcacg accacgcgcc
     3841 gctggtcgcg gcactggcgg atgcggatgc gctggcgctg tttgtcatcg aacccgaatg
     3901 gatgcgcagc ccggagtgcg atggcagcca tgtggacttt gcactggctt gcctggccga
     3961 actgcgggcc gctttggcgc tgcgcggtct gccgctgttg gtgcgcgtgg gctcggctgt
     4021 ggaagtactg gcgcaactgc acagcgaggt ggctttcacc cacctgctga gccacgaaga
     4081 aaccggcccg ggctggtcgt atgtgcggga caagcaggtg gccgcgtggt gccaatccgt
     4141 ccacgtgccc tggcaggaat tcacccagac cggcgtggtg cgccgcttgc gcacccgcag
     4201 cggctgggca cagcgctggc aggcgcgtat ggatgcacct ttgcatttgc tggacggccg
     4261 tttttccgct gcggttacgc tggaccaacc tgacctcccc acgctgtcga gcctgggggt
     4321 ggccccgcat ggcaaggtgt tgcaggctgc gggcgagcgg gctgcgcgtc acaccctgca
     4381 aagctttttg caggtgcgtg gccatgacta ccgcaaggca ctttccagcc ccttgagtgc
     4441 ggaagagggc tgtagccgcc tgagtcctca tttggcgttc ggcacgctct ccctgcgcac
     4501 ggtgcaccag gccaccgagg tggcgatcgg cagtacgcca gaccgtaccg tggcctacgc
     4561 gctgcgcggc tttgccgggc gcctgcgctg gcactgccat ttcatgcaga agctcgaaga
     4621 cgagcccgac attgaattcc acaacttcgc ccgcgtgtac gacggtctgc gcgaaaacga
     4681 tttcaacgac gaccactttg ccgcctggtg cgaagggcgc accggctacc ccatggtgga
     4741 cgcctgcatg cgttcgctcc ttgccaccgg ctggctcaac ttccgtatgc gcgccatgct
     4801 ggtgagcttt gccagctacc acctgtggtt gcattggcga ccaacgggcc tgtttttggg
     4861 gcgccagttt ctcgatttcg agcccggcat ccactggagc cagatgcaga tgcaaagcgg
     4921 caccacgggc atcaacacgc tgcgcatcta ctcgcccacc aaacaggcgc tagaccagga
     4981 cccgcaaggt ggtttcattc gtcgctgggt gccggagctc tccaaagtgc cactgcccta
     5041 tctggccgaa ccgtggaaga tgaatgtgag cgtgcagcgt atggccggtt gcatgatcgg
     5101 tgtggactat cccgccccca tcgtggacga caagctggcg atgaagaccg ccaaagaccg
     5161 tatgtatggc ttgcgcaaaa cacaggaggc gcgcgaagaa gccggcgatg tgcaggcgcg
     5221 ccatggatcg cgcaagagcg ggctcccccc ttccgggcaa cgtgccaagg cagcatcgcg
     5281 cggagccaaa ggtcctgccg caccatcacc ccaaggggac ttgttcgcat gaaaaacgac
     5341 ttcaaaggca acaaacaatc gctgcccagc aaactgtgcg ccgtgtgcgg ccgcaccatg
     5401 acctggcgca aggcctgggc caagaactgg gagtcggtgc tgtactgctc ggatgcctgc
     5461 cgcgccagga aaaccaacaa aaaagcacca tgaccccaac cgacacccac ccggccacag
     5521 ccctgcgtct tgcaccgtta ccagcgacgg tgcgcaacct ggtcatcgtg ctgggcgacc
     5581 agctcgacgc agagtcttca gcgctgcaag attttgatcc cacgcaggat gtggtgtgga
     5641 tggccgaggt ggcgcaagag tccacccatg tgtggtcggc caagcagcgc attgcggtgt
     5701 tcttgagcgc catgcgccac tttgccgacg acctgcgcag gcaaggcctg cctctggtct
     5761 acgccaggct ggacgatgcg gacacccggg gcaccctggc gttggagctg gacaaagcca
     5821 tcacccagct gcaccccgcc acgctggtca tcactgcccc aggcgactgg cgtgtgctgc
     5881 acagcctgcg cgcggtggcc gctgagcacc agctaccgct ggacctgcgc gacgaccccc
     5941 attttttcag cacggtgcgt gactttgcgg cacacgccca aggccgcaag cagctgcgcc
     6001 tggaatactg gtaccgcgag ctgcgccaga agcacggcat cctgatggac ggcaaagaac
     6061 ctgcgggtgg gcaatggaat tttgatgcgg acaaccgcga gtccttcggt aaggccgggc
     6121 cacagaatgt gcctgcgccc acccgttttg caccggacgc caccacgcaa gacgtcatcg
     6181 cactggtcca tacgcgattt gccagccacc cgggctcgct tggcagtttt ggctggccgg
     6241 tgacgcgcac gcaagcgctg gcagcgctgc aggcattcat tcaagagcgc ctgccgttgt
     6301 ttgggttgta tgaagacgcc atgtggtcgg gcgaaccgtg gctgtaccac tcgcacctga
     6361 gctgcgcgct caacctcaag ctgttgaacc cgcgtgaggt agtgcaagcc gccgaagatg
     6421 c
//
LOCUS       DAOSJM010000109         6416 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10951_length_6416_cov_5.026411, whole genome shotgun sequence.
ACCESSION   DAOSJM010000109 DAOSJM010000000
VERSION     DAOSJM010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10951_length_6416_cov_5.026411"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PKX82_05305"
     CDS             complement(<1..1128)
                     /locus_tag="PKX82_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOF51702.1"
                     /translation="MTNIIDPIAAGLQRGWKVLGGKHGALPERITCDVAIIGSGAGAG
                     ITAELLTKAGLNVVIVEEGQLKSSTDFKQKEPEAYASLYQENAGRKTKDKGITILQGR
                     SVGGTTTVNWTSSFHTPPDTLKFWQDKFGLKDYTTETLAPYFQQAQTRLNISPWATSP
                     NENNELLRRGAAKLGIKAEVIPRNVKGCYNLGSCGMGCPTNAKQSMLVTTIPFALDHG
                     ATLLVETRAEKLVIEGGRVTAMQCIAVKPNAAPVESGKIATTIVAKHFVLAGGAINSP
                     ALLMRSKAPDPHKRLGTRTFLHPVAITTAVMKDTVAAWTGAPQSIYSDHFLHTQPVDG
                     PIGFKLEVAPLHPVFFGANLPGFGEKQAELFRQYPHANVMLA"
     gene            complement(1154..1681)
                     /locus_tag="PKX82_05310"
     CDS             complement(1154..1681)
                     /locus_tag="PKX82_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51703.1"
                     /translation="MKHLPPHLQRRSFLKLGIGAAVVLAVAGGAVALIKPGLVNGRLS
                     PSARLLMARVGQAMLQGTLPADALEQQRAIDAVLQRTDDFTAGLPAHVQAELSQLFGL
                     LATAAGRRGIVGLSASWEDATAAETSAALQSMRASGTELRIQAYQGLHDIVFVPYFSG
                     EESWKSIGYPGPRAV"
     gene            complement(1698..3140)
                     /locus_tag="PKX82_05315"
     CDS             complement(1698..3140)
                     /locus_tag="PKX82_05315"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HOF51704.1"
                     /translation="MSAVPIHAATSSDAIRLELEEVLKLQRAAYFAHPYPTFAERKAD
                     LLKLKAFIKDNRDAIVDAISADYGNRSRHETLFAEIFSVVDGVEHTLKHLKTWMKPQR
                     RGVDIKNFFGAKNRVVPQPLGIVGAIVPWNFPINLSFSGLIAAFAAGNRSMVKMSENS
                     RHLAKLLIEKSPKYFPRDKLAFFDETGGVGIEFSKLKFDHILFTGSGLTGRAVMAAAA
                     PNLCPVTLELGGKSPAIICDDYPLRKAAERVLFVKYFNAGQICTTIDHIYIPEDKVKE
                     FTEMSKGIVTERYPHLSSPDFTSMIDDRSFNRVVGAMEEAQERGATLVQLIPGPKWDA
                     ATRKIAPHIVLNAPADCALRTREIFGPVLPIIPYKKIEDPVQAINDGPRPLALYPFSN
                     DKALVHHLLTHVMSGGVSVNDGLFHVGQHDLPFGGVGDSGMGHYHGYEGFVTFSKMRP
                     VFYQAPFSAVKFLWPPYGKFATKYLDFLTK"
     gene            complement(3162..3917)
                     /locus_tag="PKX82_05320"
     CDS             complement(3162..3917)
                     /locus_tag="PKX82_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOF51705.1"
                     /translation="MGIPSVKDKVSTEEWKLRVDLAACYRLVALYGWSDLVFTHISVR
                     IPGPEHHFLINPYGLMFDEITASSLVKVDQQCNKLLDSPFPVNPAGFNIHSCIHDGRP
                     DVGCVLHTHTRAGVAVSAQKCGLLPISQQSTFVIPELAYHDYEGVALREDEKPRLQAD
                     LGNKTFLMLRNHGLLVVGSTVAEAFLSMYNFENACRIQLDAQAGGELVQINPKIMETM
                     PMVIKMVTSGQGANIAWPALIRKLDRIDTLYQT"
     gene            4021..4713
                     /locus_tag="PKX82_05325"
     CDS             4021..4713
                     /locus_tag="PKX82_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF51706.1"
                     /translation="MYPKSDLLEALSINTWFAALPLAERKAMVRAGEVLTFRAGEMLY
                     RKGDAGASFVGLVEGAFKVSTLGEDGREAILAVMEPGNWFGESSLLDGLPRPHDVTAI
                     QDSILLAIGVVEFERLMRRPAFARALGALLCSRVRVLYSLVEDAMLRSTRTRIARRLL
                     ALARGDATMAVDARARVTVSQEALAMMLGITRQTLSKELKVLARDGVLSLGYGRIDIL
                     SVPELDKRGALA"
     gene            complement(4810..5388)
                     /locus_tag="PKX82_05330"
     CDS             complement(4810..5388)
                     /locus_tag="PKX82_05330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOF51707.1"
                     /translation="MSKLLVFAGSTRTQSYNRQLAKAAADLARAAGASVNLLELGDLD
                     IPLYNADLEAQGTPPDVMKLKEILDEHPAWIICSPEYNGSYTALLKNTLDWASSPVKN
                     HAYWSDSSKPFAGKVVGLLSASPGALGGLRSLSHLSPLLLNLQCWLCPQQFALSRAGS
                     AFDAQGQLKDAAHQASVAAVVDQVLWAAKRLS"
     gene            5485..>6416
                     /locus_tag="PKX82_05335"
     CDS             5485..>6416
                     /locus_tag="PKX82_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51708.1"
                     /translation="MLYPQDFDVIVVGGGHAGTEAALAAARMGCKTLLLSHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDAMGGATALATDEAGIQFRTLNSSKGPAVRATRAQADRI
                     LYKAAIRRMLENQPNLWLFQQAVDDLMVEGDGDGARVVGAVTQVGIRFRSKTVVLTAG
                     TFLDGKIHVGLNNYAAGRAGDPPAVSLSARLKELKLPQGRLKTGTPPRLDGRSIDFSK
                     CQEQPGDGMPGGMSPVMPVFSFMGKVEMHPQQMPCWITHTNERTHDIIRSGFDRSPMF
                     TGKIEGVGPRYCPSVEDKINRFADKDSHQIFLEP"
BASE COUNT         1148 a         1805 c         2198 g         1265 t
ORIGIN      
        1 ggccagcatc acgttggcgt gggggtactg gcggaacagc tcggcctgct tctcgccaaa
       61 gccgggcagg ttggcgccaa agaacacagg atgcaggggt gcgacttcca gcttgaaccc
      121 gatcggtccg tccaccggct gggtgtgcag gaaatggtcg gagtaaatcg attgcggtgc
      181 accggtccat gcggccacgg tgtctttcat gacggccgtg gtgatggcca ccgggtgcag
      241 gaaggtgcgc gtgcccagcc gcttgtgtgg gtcgggcgct ttcgagcgca tcagcagcgc
      301 cggagagttg atcgccccac ctgccaggac aaaatgtttt gctacgatag ttgtagcaat
      361 cttgccagat tctacggggg ctgcattcgg tttcaccgca atacattgca tggcggtgac
      421 acgaccacct tcgatgacca gtttctcggc ccgtgtttcc accagcagcg tggcgccatg
      481 gtccagtgca aacgggatgg tggtcaccag catggactgc ttcgcattgg tggggcagcc
      541 catgccgcag gaaccgaggt tgtagcagcc cttgacgttg cgcggaatca cttcggcctt
      601 gatgccgagt ttggctgcac cgcggcgcag cagctcgttg ttctcgttgg gcgatgtcgc
      661 ccagggcgaa atgttcagtc gggtttgagc ctgctggaag tagggcgcca gtgtttcggt
      721 ggtgtagtct ttcaggccaa atttgtcctg ccagaatttc aacgtatcgg gaggcgtatg
      781 aaaggagctg gtccagttga ccgtggtggt gccgcccaca ctgcggccct gcaggatggt
      841 gatgcccttg tccttggtct tgcgcccggc gttttcctgg tacagcgagg cgtaggcttc
      901 gggctctttc tgtttgaagt cggtactcga tttgagctgc ccctcctcga cgatgacgac
      961 gttcaggcct gctttggtca gcagctccgc ggtgatgccg gcgccagcgc cagagccgat
     1021 gatggccaca tcgcaggtga tgcgttccgg caaggcgccg tgtttgccgc cgagaacttt
     1081 ccagccgcgt tgcaggcccg ccgcaatggg gtcaatgatg ttggtcatgc gatttcagtg
     1141 gatgcagggt gactcagacg gctctggggc cgggatagcc gatggacttc caggactctt
     1201 cgcccgagaa atagggcaca aacacgatgt cgtgcagtcc ctggtaggcc tggatgcgca
     1261 gctccgtgcc ggacgcgcgc atggactgca gtgcggcgct ggtttctgcc gctgtggcgt
     1321 cttcccacga ggccgacaga cccacaatgc cgcgccggcc cgcggccgtg gccagcaggc
     1381 cgaacagttg cgagagttca gcctgcacat gcgcgggcag gccggcggtg aagtcatcgg
     1441 tacgctgcag gaccgcgtcg atggcgcgtt gctgttccag cgcatccgca ggcagcgtgc
     1501 cttgcagcat ggcctgtccg acgcgggcca tcagcagccg cgcggagggg ctgaggcggc
     1561 cattgaccag gcccggcttg atcagtgcca ccgcgccgcc agccacggcc agaacgacgg
     1621 cggcaccgat accgagcttg agaaaacttc tgcgctgcag gtggggaggc aggtgcttca
     1681 tggcagtgtg tatcgggtta cttggtcagg aagtcgaggt acttggtcgc gaacttgccg
     1741 taaggcggcc acaggaactt cacggcgctg aagggggcct ggtagaacac cgggcgcatc
     1801 ttggagaagg tgacaaaacc ttcgtagccg tggtagtggc ccatgccgct gtcgcccaca
     1861 ccaccgaaag gcagatcgtg ctggcccacg tggaacagac catcgttgac gctgacgccg
     1921 cccgacatca cgtgggtcag caggtggtgc accagcgcct tgtcgttgct gaaggggtag
     1981 agcgccagtg gacgcgggcc gtcattgatg gcctggacgg ggtcttcaat tttcttgtac
     2041 ggaatgatgg gcagcacggg gccgaagatc tcgcgcgtgc gcagcgcgca gtcggcgggc
     2101 gcgttgagca cgatgtgtgg ggcgatcttg cgcgtggccg cgtcccactt ggggccggga
     2161 atcagctgga ccagtgtggc gccgcgctct tgcgcctctt ccatggcacc caccacccgg
     2221 ttgaaggagc ggtcgtcaat catggaggtg aaatcgggcg agctcaggtg cgggtagcgc
     2281 tctgtgacga tgcccttgga catctcggtg aactccttga ctttgtcttc cgggatgtag
     2341 atgtggtcaa tggtggtgca gatctgtccg gcgttgaagt acttcacgaa caggacgcgt
     2401 tcggcagctt tgcgcagcgg gtaatcgtcg caaatgatgg caggcgattt gccaccgagt
     2461 tccagtgtga cagggcacag gttgggggca gccgcggcca tgacggcgcg gcctgtcagg
     2521 ccggatccgg tgaacaggat gtggtcgaac ttcagcttcg agaactcaat gcccacgccg
     2581 ccggtttcat cgaagaacgc cagcttgtcc cgggggaaat acttgggaga cttttcaatc
     2641 agcagcttgg cgagatggcg ggagttttcc gacatcttga ccatggagcg gttgccagcg
     2701 gcaaaggcgg cgatgagtcc gctgaagctg aggttgatgg ggaagttcca cggcacgatg
     2761 gcacccacga tgcccagagg ctggggaacc acgcggttct tggcaccgaa gaaattcttg
     2821 atgtccacac cgcggcgctg tggcttcatc caggtcttga ggtgcttgag ggtgtgttcc
     2881 acaccgtcga ccacactgaa gatttcggca aacagggttt cgtggcgcga gcggttgccg
     2941 tagtcggcgc tgatggcgtc cacgatggcg tcgcggttgt ccttgatgaa ggctttgagc
     3001 ttgagcaggt cagccttgcg ctcggcaaag gtggggtagg ggtgggcgaa gtaggctgcg
     3061 cgttgcagct tgaggacttc ttcgagctcc agacggatgg cgtcagaaga ggtggcggcg
     3121 tggatgggaa cggcggacat ggtgtggttt tatccgggaa atcaggtttg gtaaagggta
     3181 tcgatgcggt caagcttgcg gatcagtgcc ggccatgcga tgttggcgcc ctggcccgaa
     3241 gtgaccatct tgatgaccat gggcatggtt tccatgatct tgggattgat ctgcaccagt
     3301 tcgccacccg cctgggcgtc aagctggata cggcaggcgt tttcaaagtt gtacatcgac
     3361 agaaaggcct ctgccacggt ggaaccgacg accagcaggc cgtggttgcg cagcataagg
     3421 aaggtcttgt tgcccaggtc ggcctgcagg cggggttttt catcctcgcg cagggccacg
     3481 ccttcgtagt cgtggtacgc cagctcgggg ataacgaagg tggactgctg actgatgggc
     3541 agcagtccac atttttgtgc gctcacggcc acgcccgcgc gggtgtgggt gtgcagcaca
     3601 cagcccacgt cggggcggcc gtcgtggatg cagctatgga tgttgaaacc agcgggattg
     3661 accggaaacg gcgaatcgag cagcttgttg cattgctggt cgaccttgac caggctgctg
     3721 gcggtgattt catcgaacat cagcccgtag gggttgatga ggaagtggtg ctcggggccg
     3781 gggatgcgca cgctgatgtg ggtgaacacc agatcactcc agccgtacag cgccaccaga
     3841 cggtagcagg cggcgagatc gacgcgcagc ttccactctt cggtgctgac cttgtctttg
     3901 acggatggga ttcccaagtg ctgtctcctg gcgatggcaa gcgcgcacgt taacccggcg
     3961 ggcggtaatt aactgtcaaa aaatttacaa tttagggtaa ttactggaaa aaaataggcc
     4021 gtgtacccca aatccgatct tctggaagcg ctgagcatca acacctggtt tgcagccttg
     4081 cctctggccg aacgcaaagc catggtgcgc gcgggcgagg tgctcacgtt ccgggccggt
     4141 gaaatgctgt accgcaaggg ggatgcgggg gcgagttttg tgggtctggt ggaaggggcc
     4201 ttcaaggtgt ccacgctggg cgaggatggt cgcgaagcca tcctggccgt gatggagccc
     4261 ggcaactggt ttggcgaatc ctcgttgctg gatgggttgc cccgacccca tgacgtcacc
     4321 gccattcagg acagcatcct gctggcgatc ggtgtggtcg agtttgagcg gctgatgcgc
     4381 cgtccggcat tcgcacgggc gctgggcgcg ttgctctgca gccgggtgcg cgtgctgtac
     4441 agcctggtgg aagacgccat gctgcgctcc acccgcaccc gcattgcccg gcgcctgctg
     4501 gcactggccc gcggcgacgc aacgatggcc gtggatgcca gggcgcgtgt gacggtgtcg
     4561 caggaggcgc tggccatgat gctgggcatc acgcgccaga ccctgtccaa agagctcaag
     4621 gtgctggcgc gcgatggcgt gctgtcgctg ggctatgggc gcatcgacat cctttcggtg
     4681 ccggagctgg acaaacgcgg ggcgctggcc tgagttgcag gacagggcat ggatcagtca
     4741 ggtgaccgag caagctataa aaacaatagc tgcttgcgca attttgacgg gggctagagc
     4801 tgtatttggc taagaaagcc gcttggcagc ccagagtacc tggtccacca cggctgctac
     4861 gctggcctgg tgcgcggcgt ccttgagctg gccctgtgcg tcaaacgcgc tgccggcgcg
     4921 gctcaaggcg aactgttgcg ggcagagcca gcattgcagg ttgagcagca acgggctcag
     4981 gtggctgagc gagcgtaggc cgcccagtgc acccggtgac gcgctgagca atcccaccac
     5041 cttgcctgcg aatggcttgc tgctgtcgct ccagtaggcg tggttcttca cggggctgga
     5101 ggcccagtcc agggtgttct tgagcagggc ggtgtagctg ccgttgtact cgggcgagca
     5161 gatgatccag gccgggtgtt catccaggat ctccttgagc ttcatcacat cgggtggtgt
     5221 gccctgcgct tcgaggtcgg cgttgtacag cggaatgtcc aggtcgccca gctccagcag
     5281 gttcacgctg gccccggcag cccgggccag atcggctgca gctttggcca gctggcggtt
     5341 gtaggactgg gtgcgggtgc tgccggcgaa gacaaggagt ttgctcatgg ccccattgtc
     5401 gtcaataaac agggtagccg gtaaaatcgg cctctccctg actgcgccgg gcgcgcgttt
     5461 ctttctgcgt ccatttggaa caccatgctt tacccacaag atttcgatgt catcgtcgtt
     5521 ggcggcggcc atgccggaac cgaggccgcg ctggccgcgg cccgcatggg ctgcaagacc
     5581 ctgctgctga gccacaacat cgagacgctg gggcagatga gctgcaaccc gtccatcggc
     5641 ggtatcggca agggccacct ggtcaaggag gtcgatgcca tgggcggcgc cacggcgctg
     5701 gccaccgacg aggccggcat ccagtttcgc accctgaact ccagcaaggg gcctgccgtg
     5761 cgtgccaccc gcgcgcaggc ggaccgcatt ctgtacaagg cggcgatccg ccgcatgctg
     5821 gaaaaccagc ccaacctctg gctgttccag caggcggtgg acgacctgat ggtggaaggc
     5881 gatggtgatg gtgcgcgcgt ggttggtgcc gtgacccagg tgggcatccg cttccggtcc
     5941 aagacggtgg tgctgacggc gggtacgttt ctggatggca agatccatgt ggggctgaac
     6001 aactacgccg cgggcagggc aggggatccg ccggccgtgt cgctgagtgc gcggctgaaa
     6061 gaactcaagc taccgcaggg tcgcctgaaa accggcaccc cgccgcgact cgatggccgc
     6121 agtatcgact tcagcaaatg ccaggagcag cccggcgacg gcatgcccgg tggcatgagt
     6181 ccggtgatgc ccgtgttcag tttcatgggc aaagtggaga tgcacccgca gcagatgccg
     6241 tgctggatca cgcacaccaa tgagcgcacc cacgacatca ttcgcagcgg ctttgaccgc
     6301 agcccgatgt tcaccggcaa gatcgaaggc gtgggcccgc gctattgccc gagtgtggaa
     6361 gacaagatca accgctttgc cgacaaggac agccaccaga tttttctgga gcccga
//
LOCUS       DAOSJM010000110         6410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10953_length_6410_cov_4.110307, whole genome shotgun sequence.
ACCESSION   DAOSJM010000110 DAOSJM010000000
VERSION     DAOSJM010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6410
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10953_length_6410_cov_4.110307"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="PKX82_05340"
     CDS             complement(<1..559)
                     /locus_tag="PKX82_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HOF51709.1"
                     /translation="MQTKHKFRIATLAAALVAGCASVPGSADLDKLTTAIVKASFRDQ
                     GIVKASVLDTDETNRVCSAADVSGKPLDEETARRIEAINLQTVKAPPNGKYLGDWKEG
                     EKIAQSGRGLTYTDDAKTVNGGNCYNCHQIDKKEISFGTIGPSLTNYGKIRGVTDPAS
                     PQAKPIVEYTWGKIWNAKAYNACSNM"
     gene            complement(574..1374)
                     /gene="soxA"
                     /locus_tag="PKX82_05345"
     CDS             complement(574..1374)
                     /gene="soxA"
                     /locus_tag="PKX82_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HOF51710.1"
                     /translation="MTKTLVVASLVAVASSAVWAQKSAAVQSIEEYRAMLQDGNPADL
                     FEAKGEGLWKQKRGPKNASLEQCDLGKGPGVFKGAFVELPRYFADTGRVQDLESRLLT
                     CMETLQGLNAAEIAKTPFGKGEQNNMTALATWIAAESKGMKFNLPQAHVKEKTFYEVG
                     KRLFFQRGGPHDFSCASCHGENDKRIRLQDLPNLTKNPGDGVGVAAWPAYRVSNGQMW
                     GMQQRLNDCYRQQRFPYPGFGSDATIALGVYLGVNSQGSASVAPAIKR"
     gene            complement(1462..1773)
                     /gene="soxZ"
                     /locus_tag="PKX82_05350"
     CDS             complement(1462..1773)
                     /gene="soxZ"
                     /locus_tag="PKX82_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HOF51711.1"
                     /translation="MADPMRIRAQAAGDKATVRVLMAHEMEGGQRKDASGKIVPAWYI
                     QEVTATHNGKPVLTAEWGPAVSKNPFLQFVVKGAKAGDKVGVSWKDNRGDTRTDEATV
                     S"
     gene            complement(1830..2285)
                     /gene="soxY"
                     /locus_tag="PKX82_05355"
     CDS             complement(1830..2285)
                     /gene="soxY"
                     /locus_tag="PKX82_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HOF51712.1"
                     /translation="MQTRRNALKKSAAVAGLLASTGMFPQFAFAFNTAAFDAKSVADA
                     LKALGAGAPVESKDVSITAPDIAENGSVVPVGVATTLAGVKSMLIMVEKNPATLTALF
                     NVTDSVEPAFQARVKMGQSSDVYAIALMKDGKVLFAKKEVKVTLGGCGG"
     gene            complement(2318..3388)
                     /locus_tag="PKX82_05360"
     CDS             complement(2318..3388)
                     /locus_tag="PKX82_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOF51713.1"
                     /translation="MSSFRNAVIAAAVMAAAGGAFAQADSRFPGIGRAATPKEVAAWD
                     IDVRPDFKGLPKGSGSVAKGQDIWESKCAHCHGVFGDSGEVFSPVVGGTTAEDVKTGR
                     VAKLKDSTVGRTTFMKVATLSTVFDYINRAMPWTAPKSLSTDEVYAVTAFLLNLSGVV
                     PDDFTLSDKNIADVQKRMPNRNGMTTQHAMWPGKEFNGVKAPDVKNVACMKDCATEPS
                     VGSFLPDFARNAHGNLAEQNRMIGPQRGADTTVPEGKAPAKVVESAKPAAASGGADAK
                     AAIALLTKHSCVACHGVDKKIVGPGFNEVAKKHADKADYLASKIKSGGSGVWGPIPMS
                     AQALPEADARVIAAWIAAGAAK"
     gene            complement(3372..4736)
                     /gene="soxC"
                     /locus_tag="PKX82_05365"
     CDS             complement(3372..4736)
                     /gene="soxC"
                     /locus_tag="PKX82_05365"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HOF51714.1"
                     /translation="MKQDDLSAGRIIRAPENFLSREGIKTVFAEGKKGRRDFIRSAFA
                     AAAAGVAAPAVLAQGNPVPKEGGDTNILNLPEHATGLGQSVVTDGYGKPSKYETNVQR
                     RQSPGLTQTNQASVSFSPLQSLFGIVTPSGLHFERHHQGWWDIDPSKHRFMVNGMVKT
                     PKVFTMDEIMRLPSVSRFHFIECGANSGAEWGNVAVPTAQYTHGMISCSEFTGVPLIT
                     LLELAGADLKNGKFVLAEGGDGSSMTRTIPMSLITSGEVFVAYGQNGEMLRPENGYPL
                     RLVVPGVQGVSWVKYLRRVEVGDQPYATKDEAIHYIDLQPSGLHRQYTNIQECKSVVT
                     TPSGGQVLLDKGFYNITGLAWSGRGKVKKVDVSVDGGRNWRPARLESPVMSKALTRFN
                     VDWVWDGKPAIIQSRATDETGYVQPTYRQLRAVRGSRSIYHNNAIQSWLVQENGEVKN
                     VQLS"
     gene            complement(4838..5158)
                     /locus_tag="PKX82_05370"
     CDS             complement(4838..5158)
                     /locus_tag="PKX82_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51715.1"
                     /translation="MKHSIAQLVALLAVAGTASAQVPVIFQGADLRLGEKLISENRCV
                     ACHQQKVGGDGSSIFRPAGKINTAGYLRGMVEQCNTELNLGFFPEEVTAVAAVLNRDH
                     YKFK"
     gene            complement(5203..5775)
                     /locus_tag="PKX82_05375"
     CDS             complement(5203..5775)
                     /locus_tag="PKX82_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOF51716.1"
                     /translation="MRISTLVNRLTVLSVALVMTGCGTVSTLGKLEDGAGAEASRMWD
                     RWVEADGDIAVATTWERKVKPGVTGDDIEQILRSVATERNMKEVGILPLSKELEARTG
                     KKQKLLTVYSFCTPSTAREMVDFSPHMAAYLPCRISVVEREDGLWLYTLNMDMMVKMG
                     RKLPSPLKENAWAVRSTIFEMMERASRGEF"
     gene            5989..6315
                     /locus_tag="PKX82_05380"
     CDS             5989..6315
                     /locus_tag="PKX82_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HOF51717.1"
                     /translation="MIKKIAPAPSSDTPTPDLAGGYRVDHEKDMVNRLRRIEGQVRGL
                     VDMIQSGRSCEDIATQMSATRKAMDKAFYRMMTCTLIEAVHDSKTDEEAIAEVERSAR
                     LLEKFA"
BASE COUNT         1296 a         1984 c         1783 g         1347 t
ORIGIN      
        1 gcatgttgga acaggcgtta taggccttgg cgttccagat cttgccccag gtgtattcca
       61 cgatgggctt ggcctgcgga ctggccggat cggtcacgcc acggatcttg ccgtagttgg
      121 tcaggctcgg gccaatggtg ccaaaggaga tttccttctt gtcgatctgg tggcagttgt
      181 agcaattgcc gccgttgacc gtcttggcat cgtcggtgta cgtcaggcca cgcccgctct
      241 gtgcaatttt ctcgccctcc ttccagtcgc ccaggtactt gccgttgggc ggcgccttga
      301 cggtctggag gttgatggct tcaatgcgcc tggccgtctc ctcgtccagc ggcttgccgc
      361 tcacgtcggc agcactacag acgcgattgg tctcgtcagt gtccagcacc gacgccttga
      421 cgatgccctg atcgcggaag gatgctttga caatagcggt ggtcaatttg tccagatcgg
      481 ccgaaccggg aaccgaggcg cagcccgcca ccagggctgc tgcgagcgtt gcgatacgaa
      541 atttatgttt ggtttgcatg gtcaatccct cgctcaacgc ttgatggccg gtgcgacaga
      601 cgcagagccc tggctgttca cgcccaggta cacacccagc gcaatggtgg catcgctgcc
      661 aaagcccgga tacggaaaac gctgctgacg gtagcagtcg ttcagacgct gctgcatgcc
      721 ccacatctgc ccgttggaca cacggtaggc aggccaggcg gcaacgccca cgccatcacc
      781 cgggttcttg gtcaggttgg gcaggtcctg cagccggatg cgcttgtcat tctcgccatg
      841 gcaagaggca cacgagaaat cgtgggggcc accgcgctgg aagaacaggc gcttgcccac
      901 ttcgtaaaag gtcttttcct tcacatgggc ctgtggcagg ttgaacttca tgcccttgga
      961 ctcggctgcg atccaggtcg ccaatgccgt catgttgttc tgttcgccct tgccaaaggg
     1021 ggttttggca atctcggcgg cgttgagtcc ctgcagggtc tccatacagg tcaacaggcg
     1081 cgactccagg tcctgcacac ggccggtatc ggcaaaatac cggggcaact cgacgaaggc
     1141 gcccttgaat acgcccgggc ctttgcccag atcgcactgc tccagcgacg cattcttggg
     1201 gccacgtttt tgcttccaca gcccctcacc cttggcctcg aacagatcgg ccggattgcc
     1261 gtcctgcagc atggcgcggt attcttcaat cgattgcacc gccgcacttt tctgggccca
     1321 gactgcactg ctggccaccg ccaccagcga agccacgacc agtgtttttg tcatcctcat
     1381 agcgcgccct cttgtattga taaaccaacg aacaaagggg aagcctgcgc ctcccctgca
     1441 agaaacacga ttcctagcgc atcaggaaac cgtggcttcg tccgtgcggg tatcgccgcg
     1501 gttgtctttc cagctcacac caaccttgtc gcccgccttg gcacccttga ccacgaactg
     1561 caggaaaggg ttcttggaca cagcggggcc ccactctgcc gtcagaacgg gtttgccatt
     1621 gtgggtggca gtgacctcct ggatgtacca ggccggaacg atcttgccgg aggcgtcttt
     1681 gcgctgaccg ccctccatct catgggccat caacacacgc acggtggcct tgtcgccagc
     1741 tgcctgggcg cgaatacgca tcggatctgc cattttgaaa ctcctgaatc tgtatgaaaa
     1801 atattgaaaa cgtaggtgac ggggggggct tagccgccgc atccacccag agtgaccttg
     1861 acttccttct tggcgaacag cactttgccg tccttcatca gggcaatcgc atagacatcg
     1921 gaggactgcc ccatcttgac gcgggcctgg aatgcaggct ccacgctgtc ggtcacattg
     1981 aacagggcgg tcagtgtggc ggggttcttt tccaccatga tcagcatgct cttcacaccc
     2041 gccaaggtag ttgccacgcc aacgggaacg acagaaccgt tctcggcgat gtcgggggcg
     2101 gtgatgctca catccttgct ttccaccgga gcacctgcgc cgagcgcttt cagcgcatcg
     2161 gccacggact tggcatcgaa cgctgcggtg ttgaacgcaa aagcgaattg ggggaacatg
     2221 cccgtggagg ccagcaggcc agccactgcg gcggatttct tgagggcatt tctgcgagtt
     2281 tgcataggaa gtctcctgag ggttaattga gaaaaattta cttggcggcg ccagctgcaa
     2341 tccatgcagc aatgactcta gcatccgctt ccggcaatgc ctgtgccgac atgggtatgg
     2401 gtccccacac gccagaaccg cccgacttga tcttggaagc caggtagtcc gccttgtccg
     2461 cgtgcttctt cgcaacttcg ttgaagcccg gacccacaat cttcttgtcc acaccgtggc
     2521 acgccacaca gctgtgcttg gtcagcaatg caatggccgc tttggcatcg gcaccaccgg
     2581 aagctgcagc aggtttggca gactcaacca ccttggctgg cgccttacct tccggcaccg
     2641 tggtgtcagc tccgcgctgc ggaccaatca tgcggttttg ctccgccaga ttgccgtgcg
     2701 cattacgtgc aaagtccggc aggaaagagc ccacgctggg ctcggtcgcg cagtctttca
     2761 tgcaggccac gttcttgaca tcgggcgcct tgacaccatt gaactccttg ccgggccaca
     2821 tcgcgtgctg ggtggtcatg ccattgcggt tgggcatgcg tttttgcaca tcggcaatgt
     2881 tcttgtcgga cagtgtgaag tcatccggca ccacgccact gaggttcagc agaaaggccg
     2941 ttacggcata cacctcgtcg gtcgacaggg acttcggagc ggtccatggc atggcacggt
     3001 tgatgtagtc aaagacagtg gacagcgtgg cgaccttcat gaaggttgta cgacccaccg
     3061 tcgaatcctt cagcttggca acacggcccg tcttcacatc ctctgcagtg gtgccgccca
     3121 ccacagggct gaacacttcg ccagagtcac caaaaacacc gtggcaatgc gcgcatttcg
     3181 attcccagat gtcctgtcct ttggctaccg aaccggagcc cttgggcaag cccttgaaat
     3241 ccggacgcac atcaatatcc caggcagcca cttctttcgg tgttgccgcg cggccaatgc
     3301 ctggaaatct ggaatcggct tgtgcaaacg caccgccggc cgcagccatc actgcagctg
     3361 cgatcactgc attacgaaag ctggacattt ttcacctcac cgttttcctg aacgagccat
     3421 gactggatgg cgttgttgtg gtagatcgac cggctgccgc gcaccgcgcg caactggcgg
     3481 taggttggct gcacatagcc cgtctcatca gtggcccggc tctggatgat ggcgggcttg
     3541 ccatcccaca cccagtccac attgaagcgt gtcagcgctt tggacatgac cggtgactcc
     3601 agacgcgctg ggcgccagtt gcggccacca tcgaccgaga cgtccacctt cttgaccttg
     3661 ccacgacccg accaggccag accggtgatg ttgtagaaac ccttgtccag caacacctga
     3721 ccgcccgagg gagtggtcac cacgctcttg cattcctgga tattggtgta ctggcggtgc
     3781 aggccactgg gctgcaaatc aatgtagtgg atggcctcat ccttggtggc atagggctgg
     3841 tcgcctactt ccacgcggcg caggtatttc acccagctca cgccctgcac accgggcacc
     3901 accaggcgca acggatagcc gttctcgggg cgcagcattt caccgttctg gccataggcc
     3961 acgaacactt cgcccgacgt gatcaggctc atgggaatgg tgcgggtcat ggacgaacca
     4021 tcaccacctt cagccaatac aaacttgcca ttcttcaggt ctgcacccgc cagctccagc
     4081 agggtgatga gcggaacacc ggtgaactcg ctgcaggaga tcatgccgtg ggtgtactgg
     4141 gcggtcggca ctgccacatt gccccactcc gcgccggagt tggcaccaca ctcgatgaag
     4201 tgaaagcgcg agacagacgg caggcgcatg atctcgtcca tggtgaatac cttgggcgtt
     4261 ttcaccatgc cgttgaccat gaagcggtgc ttggacggat caatatccca ccagccctgg
     4321 tggtggcgtt caaagtgcaa gccgctcggc gtgacgatgc caaacagcga ttgcagcggc
     4381 gaaaacgaca cagaggcctg gttggtctgt gtcaggcccg gactctggcg gcgctgcaca
     4441 ttggtttcat atttggaggg tttgccatag ccatcggtca ccacactctg ccccagaccg
     4501 gtggcgtgct ccggcaggtt cagaatattg gtgtcgccgc cctccttggg gacgggattg
     4561 ccctgggcca ggacggccgg ggccgcaacg ccggctgctg cagcggcaaa ggcactgcga
     4621 atgaaatcgc ggcggccctt tttgccttcg gcaaagaccg tcttgatgcc ttcccggctt
     4681 aaaaaattct cgggcgccct gatgatgcgg cccgcagaaa gatcatcttg cttcacgcgg
     4741 tctccttgga tctaaataac tctatattcg aaacaactga tatatatcat ccgtggccta
     4801 tctgacttca gcatgacagc cctgcaccga agcgcattca cttgaacttg tagtgatccc
     4861 ggttcaggac tgccgccacg gcggtcactt cttcagggaa aaaacccagg ttgagctcgg
     4921 tgttgcactg ctccaccatg ccccgcaaat agccagccgt attgattttc ccggccggcc
     4981 gaaagatgga cgacccatcg ccgccgacct tctgctggtg gcacgccaca caacggtttt
     5041 cagaaatcaa tttctcgcca agcctcaggt ctgcaccctg gaaaatcaca ggcacctgcg
     5101 ctgacgccgt accggcgact gcaagcagcg cgaccagctg agctatggaa tgtttcatga
     5161 atgaaccttg tctcgttgtc acccggcgcc cacgagcccc ggtcagaact ctccgcggga
     5221 ggcgcgttcc atcatctcaa aaatggtgct gcgcacagcc caggcgtttt ctttcaatgg
     5281 ggatggcagc ttgcgcccca tcttgaccat catgtccatg ttcagggtgt agagccacaa
     5341 gccatcttcc ctttccacaa cggaaatacg gcagggaaga taggccgcca tgtgggggct
     5401 gaagtccacc atctcgcggg ctgtcgacgg agtgcagaaa gagtacaccg tcagcagctt
     5461 ctgcttcttg ccagtgcggg cttcgagttc cttggacagc ggcaggatgc ccacttcctt
     5521 catgttgcgt tcggtggcca cactgcgcag aatctgctcg atatcgtcac cggtcacacc
     5581 gggtttgacc ttgcgctccc atgtagtggc tactgcaata tcaccgtccg cctccaccca
     5641 gcgatcccac atgcgactgg cctccgcccc cgcaccgtct tccagcttgc caagcgtgct
     5701 gaccgtgccg cagccagtca tcactagcgc gactgacagg actgtcaaac ggttgaccag
     5761 ggtggaaatt ctcatgggca ctccagtagg gaaagggtcg ttggaactcc agaaacctgc
     5821 ctgcaacgga ccaatctctg atgtgtatca atcattccaa aaatataccc cccagggaat
     5881 aggtaagaac cctataccgc ccggggtacg gtatgcaagc gccattggaa actccaccta
     5941 catctggcac actcctccat cagttcctta cgtattgctt aaaccaccat gatcaagaaa
     6001 attgcacccg ccccctcctc tgatacgcca actcccgatc tggccggcgg ctaccgcgtc
     6061 gaccacgaga aagacatggt caaccgcttg cgccggatcg aaggccaggt acgtggcctg
     6121 gtggacatga tccagagcgg gcgcagctgc gaggacatcg caacgcaaat gtcggcgaca
     6181 cgcaaagcca tggacaaggc gttctaccgc atgatgacct gcaccctgat tgaagcggtg
     6241 catgactcca agaccgatga agaggctatt gcagaagtgg agcgctcggc ccgactgctg
     6301 gaaaaatttg cctgaacctg tcgggcagcc actacaagcg aatcaaaaca agctctagcc
     6361 cctgtcgctt ataagtagtt tgctatacta aatgtagcaa accaactagg
//
LOCUS       DAOSJM010000111         6406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_10961_length_6406_cov_5.243741, whole genome shotgun sequence.
ACCESSION   DAOSJM010000111 DAOSJM010000000
VERSION     DAOSJM010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10961_length_6406_cov_5.243741"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="PKX82_05385"
     CDS             complement(<1..1217)
                     /locus_tag="PKX82_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease catalytic domain-containing
                     protein"
                     /protein_id="HOF51718.1"
                     /translation="MQFQRLHHRADLVRIAIHAMLERGLEPEFPIEIDRQLATISGPG
                     RERDTAIHDLTDLLWCSIDNDDSMDLDQLTASEVLEQGAVRIRVAIADVDALVQKDTP
                     IDAHARTNTTSVYTSARIFPMLPEKLSTDLTSLNEKEDRLAVVTEMVFNPDGSLAGST
                     VYRALVHNRAKLAYDATSAWLDGRGEMPSAMAAVAGMEEQVRTQDAVAQQVRARRHSE
                     GSLEFETFQPRAVFEGEKVVDIALQEHNRARQLIEELMIATNTCTARFLAGRGGLSLR
                     RVVRSPERWLRIVAVAKDYGDTLPTEPDSVALGAFLAKRRRIDPLRFPDLSLVIVKLM
                     GAGEYVVEGPHGAAIGHFGLALRDYTHSTAPNRRYPDLITLRLIKAALAGVPPPYTDA
                     ELATLALHCTRQE"
     gene            complement(1242..2459)
                     /locus_tag="PKX82_05390"
     CDS             complement(1242..2459)
                     /locus_tag="PKX82_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOF51719.1"
                     /translation="MASGKILVAQGGGPTAVINQSLVGVALEARRFRGIEHVYGARHG
                     VRGIVNEDFVHLTQETSHNLEMVANTPSSALGSTRDKPDAKYCHEIFKVLRAHEIGHF
                     FYIGGNDSSDTVRIVSAEAQKAGYPLRCVHIPKTIDNDLVGNDHTPGFPSAARFVAQA
                     FAGANLDNAALPGVYVAVVMGRHAGFLTAASALGKKFADDGPHLIYLPERTFSLEKFL
                     ADVKATYERLGRCVIAVSEGIHDSSGAPIATLLAKDLEHDDHGNVQLSGSGALADLLC
                     EEIKSKLGIKRVRGDTLGYLQRSFIGCVSDVDQREAREVGEKAVQFAMWGQQDGSVAI
                     KRTGFYSVDYELLPLDAVAGKTRVMEDAFITASGTDVTDAFRLYLRPLLGSGMPDAYR
                     LRTNPVTKVLRPA"
     gene            complement(2481..4256)
                     /gene="ptsP"
                     /locus_tag="PKX82_05395"
     CDS             complement(2481..4256)
                     /gene="ptsP"
                     /locus_tag="PKX82_05395"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOF51720.1"
                     /translation="MTFAIHGLTVSRGIAIGRAVLVASSRVDVAHYFIEPGQVDAEVD
                     RVRIGRDVVVEEIHRLQESISHMGPKEAPHELSALLDVHLMLLQDVELINGVKHWITD
                     RLYNGEWALTTQLEIIVRQFDEMEDAYLRERKADMEQICERILRAMKGADSPVVAHVP
                     RAARKPSQQDLLLDDTVDVPLVLIAHDLSPADMLQFKQSVFAGFITDVGGKTSHTAIV
                     ARSMDIPAVVGARTCSQLVRQDDWVIIDGDAGVVIVDPSPIILAEYGFKQRQGELERG
                     RLQRLLHTSAVTMDGEKVQLLANIEMPEDAPGALKAGALGVGLFRSEFLFMGRQGRLP
                     DEEEQYQAYKKAVEGMQGLPVTIRTVDVGADKPLDESVRDDAHLNPALGLRAIRWSLA
                     DPAMFLTQLRAILRAAAHGQVHLLVPMLAHAREIRQTLAHIDHARAMLDNRGMAYGPV
                     KLGAMIEIPAAALSLPLFLRHFDFLSIGTNDLIQYTLAIDRSDESVAHLYDPLHPAVL
                     RLVADTIAQCRAQGKPVSVCGEMAGDVGLTRLLLGLGLRSFSMHPAQILAVKQEVLRA
                     DTVKLTAWAQQVVAAEDPALAMSAP"
     gene            complement(4347..4616)
                     /locus_tag="PKX82_05400"
     CDS             complement(4347..4616)
                     /locus_tag="PKX82_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOF51721.1"
                     /translation="MIRTTTTIVNKLGLHARASAKLTKLAGSFPCEVWMSRGERRVNA
                     KSIMGVMMLAAGLGSEVTLETDGEREQEAMDALLALMQDKFGEGE"
     gene            complement(4585..5031)
                     /locus_tag="PKX82_05405"
     CDS             complement(4585..5031)
                     /locus_tag="PKX82_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HOF51722.1"
                     /translation="MNTGILIIAHAPLASALRAGVLHVFPDVADGIIALDVPPSEPTE
                     TTILNARAMLAQLQTPNTLVLTDVFGATPCNVAQKVIDGVHSRLVAGVNLPMLLRTVN
                     YCHEPLDVLVARALAGGTQSIMQVAVTAPQNQQRKNNDQNHHDHSQ"
     gene            complement(5056..6279)
                     /locus_tag="PKX82_05410"
     CDS             complement(5056..6279)
                     /locus_tag="PKX82_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF51723.1"
                     /translation="MTPLPRRQAVSIFLVFAFAYFISTLIRAVTATLAPTLVTEFSLN
                     ARDLGLLAGGYFFGFALTQLPLGTWLDRFGPKRVILGFLGIAVVGAFAFAQASSFQWL
                     LASRVLCGVGVSACLMAPLTGYRRWLAPENLMRANSWMLMVGALGMVASTLPVQWIVS
                     VAGWRPLFIALSAMVALAMVLIYLQVPHWHAAPTSVSSQSTASRGYGEVWRNPYFRKM
                     APLGFFNYGGMVAMQTLWAAPWMVKVAGYTPAQAAMGLFWINVSMLLAFLAWGSLNPT
                     LARKGYSTDRLLVIGMPVVLAIFAIIIIAGGAISTEAGFAWLLFCVTSTVGGQVQPAV
                     GMAFRAELAGRALSAFNLVIFAGVFSVQWGVGLLIDGFRTTGMSEVVAYQGALGVYGL
                     CCLGSYLYFLTAKKP"
     gene            complement(6283..>6406)
                     /locus_tag="PKX82_05415"
     CDS             complement(6283..>6406)
                     /locus_tag="PKX82_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4197 family protein"
                     /protein_id="HOF51724.1"
                     /translation="VTGKALDGLYFMISEEEKKIRQNPVGYGSAILTKVFGALQ"
BASE COUNT         1315 a         2041 c         1874 g         1176 t
ORIGIN      
        1 tcttcctggc gggtgcaatg caatgccagc gtggccagtt cagcatcggt atagggtggc
       61 ggtaccccag ccagcgctgc cttgatcaga cgcagggtaa tgaggtcagg ataacggcgg
      121 ttgggtgcag tcgaatgggt gtagtcacgc agggccaaac caaaatgccc gatcgccgcc
      181 ccgtggggcc cttccaccac gtattccccc gcacccatga gtttgacgat gaccagagac
      241 aaatccggaa accgcaaggg gtcgatccgg cggcgcttgg ccagaaacgc ccccaacgcc
      301 accgaatcag gttcggtcgg cagcgtgtcg ccatagtcct tggctacggc cacaatgcgc
      361 agccagcgtt cgggtgaacg caccaccctg cgcagcgaga gcccgccgcg tcccgcaaga
      421 aaccgtgcag tacaggtatt cgtggcgatc atcagctctt cgatcaactg gcgcgcacgg
      481 ttgtgttcct gcagggcaat atccaccact ttttcaccct cgaacacggc acggggctgg
      541 aaagtctcga actccagtga gccctcgctg tgcctgcgcg cacgcacctg ttgtgccacg
      601 gcgtcctgtg tccgtacctg ttcttccatg ccggccactg cggccatggc ggacggcatc
      661 tcgccccggc catcaagcca ggccgaggta gcgtcgtagg cgagcttggc ccggttatgc
      721 accagagcgc ggtacaccgt ggaaccggca agggacccgt ccgggttgaa caccatttcg
      781 gtgaccaccg ccagccggtc ttccttttcg ttcagcgatg tcagatccgt ggacagcttc
      841 tcgggcagca tggggaatat gcgcgccgag gtgtacaccg atgtggtgtt ggtgcgggca
      901 tgggcatcga tcggcgtgtc tttttgaacc agtgcgtcca cgtctgcaat ggccacccga
      961 atgcgcacgg cgccctgctc cagtacctcg gatgcggtca actggtccag gtccatcgaa
     1021 tcatcgttgt caatggaaca ccacaacaag tcagtgaggt cgtgaatggc cgtgtcacgc
     1081 tcccgaccgg gaccgctgat ggtcgccagt tggcgatcaa tctcgatggg gaactccggc
     1141 tccagtccgc gctcaagcat ggcatggatg gcaatgcgta ccagatcggc acggtggtga
     1201 aggcgttgaa actgcatcag cccactatac gcggcgacaa actacgccgg acgcagcacc
     1261 ttggtcaccg gattggtgcg cagccggtag gcgtctggca tgcccgagcc cagcagcggc
     1321 cgcagataga gccggaaagc gtccgtcaca tcggtgccgc tggcggtgat gaacgcatct
     1381 tccatcaccc gcgttttccc ggctaccgca tccagcggca gcaactcgta gtcgaccgaa
     1441 taaaacccgg tgcgcttgat ggccaccgag ccatcctgct gcccccacat ggcgaactgc
     1501 accgcttttt cacccacctc gcgggcttcg cgctggtcca catccgaaac acacccgatg
     1561 aatgagcgct gcaaatagcc cagggtatcg cctcgcaccc gtttgatacc gagcttgctc
     1621 ttgatttctt cacacagcag atctgccagt gcaccactgc ccgagagttg cacgttgcca
     1681 tggtcatcgt gctccaggtc tttggcaagc agtgtggcaa tgggtgcgcc cgaactgtca
     1741 tggatacctt cggacaccgc aatcacacag cgccccaatc gttcgtaagt tgccttgacg
     1801 tcggccagga atttttccag cgaaaaagta cgctcgggca ggtagatcag gtgcgggccg
     1861 tcgtcggcaa acttctttcc caaggcggat gccgcggtca gaaatcccgc atgccgcccc
     1921 atgaccacag ccacatacac cccgggcaag gctgcgttgt ccagattggc gcccgcaaag
     1981 gcctgcgcca caaagcgcgc agccgagggg aacccggggg tatggtcgtt gcctaccagg
     2041 tcgttgtcga tggttttggg aatatgcaca cagcgcaagg ggtagcccgc cttttgggcc
     2101 tctgcactga cgatgcgtac ggtatcggac gagtcgttgc caccgatgta gaagaagtgc
     2161 ccgatctcgt gcgcccgcaa taccttgaag atttcgtgac agtacttggc atcgggcttg
     2221 tcgcgggtcg aacccagggc agaagaaggt gtgttggcca ccatctcgag gttgtggctg
     2281 gtctcctgcg tgagatggac aaagtcttcg ttgacgattc cccgcacccc atggcgtgca
     2341 ccgtacacat gttcgatgcc acgaaagcgc cgggcctcca aagccacgcc taccagcgac
     2401 tggttgatta ccgcagtggg accgccaccc tgcgccacaa gaatttttcc ggatgccatg
     2461 gtgctctccc tctcgaatgt tcagggcgca gacatcgcca gcgcgggatc ttccgccgcc
     2521 accacctgtt gcgcccaggc ggtcagcttg accgtatccg cacgcaagac ttcctgcttg
     2581 accgccagga tctgcgcggg gtgcatggaa aaactgcgca ggcccaggcc cagcaacagc
     2641 cgcgtcagac ccacatctcc agccatttcg ccacacacac tcaccggctt gccctgcgca
     2701 cggcactgcg caatcgtgtc cgccaccagg cgcagcacgg cagggtgcag cgggtcgtac
     2761 aagtgggcca cggactcgtc tgaccggtca atcgccaggg tgtactggat cagatcgttg
     2821 gtgccaatgg acaaaaagtc aaagtggcgc aggaacagcg gcaggctcag cgccgccgcg
     2881 ggaatttcta tcatggcgcc cagtttgacc gggccgtacg ccatgccacg gttgtccagc
     2941 atggcacgtg catggtcgat gtgggccagc gtctggcgaa tctcccgcgc atgggccagc
     3001 atgggtacca gcaggtgcac ctgcccgtgg gcagcggccc gcaggatcgc acgcaactgg
     3061 gtcaggaaca tggccgggtc cgccagactc cagcggatgg cacgcaggcc cagcgccggg
     3121 ttcaggtggg catcatcgcg caccgactcg tccagcggct tgtccgcgcc cacgtccacc
     3181 gtgcgaatcg tgaccggcaa cccctgcatg ccttcgactg cttttttgta agcttggtat
     3241 tgttcctctt cgtccggtaa ccggccctgg cgccccatga acaggaattc gctgcggaac
     3301 aggcccacgc ccagggcgcc cgccttcagg gcaccgggcg catcctccgg catttcgata
     3361 ttggccagca actgcacctt ttcaccgtcc atggtgacgg ccgaggtatg cagcaggcgc
     3421 tgcaggcgtc cgcgctccag ttcgccctgg cgctgtttga aaccgtattc ggccaggatg
     3481 atgggcgagg ggtcgacgat gactacaccg gcatcgccgt cgatgatgac ccagtcgtcc
     3541 tggcgtacca gctggctgca ggtacgcgcc cccaccacgg cggggatatc catgctgcgc
     3601 gccacgatgg cggtgtgcga ggtcttgccg cccacgtcgg tgatgaaacc ggcaaacaca
     3661 ctttgcttga actgcagcat gtcggccggc gacaggtcat gcgcaatcag caccagcggc
     3721 acatccaccg tgtcgtccag cagcaggtcc tgctgcgacg gtttgcgcgc ggcccgaggc
     3781 acatgcgcca ccaccggcga gtcggcaccc ttcatggcgc gcaggatgcg ctcgcagatc
     3841 tgctccatgt cggccttgcg ctcacgcagg taggcgtctt ccatttcatc aaactggcga
     3901 acgatgattt ccagctgggt cgtcaacgcc cactcaccgt tgtagaggcg atcggtaatc
     3961 cagtgtttga cgccattgat cagctccacg tcctgcaaca gcatgaggtg cacatccagc
     4021 agcgcagaaa gctcgtgcgg cgcctccttg ggccccatat ggctgatgct ttcctgcaga
     4081 cggtggattt cctccaccac gacatcgcgg ccgatccgca cccggtccac ttcagcgtcc
     4141 acctggccgg gctcaatgaa atagtgggca acatccaccc ggctcgacgc aaccagcacc
     4201 gcacgcccaa tggcaatgcc acgggaaact gtgagtccgt ggatggcgaa tgtcattgcg
     4261 aacgcagcgc agggccgccc caagcaagtt cagccccctt ggggggcagc gaagcacgcg
     4321 aagcggcaag cgtgggggcc ggcatgttac tcaccttcgc caaatttgtc ctgcatcagc
     4381 gccagcaacg catccatggc ttcctgctcg cgctcgccat cggtttccag cgtcacttcc
     4441 gagcccagac cagcggcgag catcatgacg cccatgatgc tcttggcgtt cacccggcgt
     4501 tcgccgcgcg acatccagac ttcgcacggg aagctgcccg caagcttggt cagtttggcc
     4561 gacgctcgtg cgtgcagtcc gagcttattg actatggtcg tggtggttct gatcattgtt
     4621 ctttctctgc tgattctggg gtgcagtgac ggccacctgc atgatgcttt gtgtcccacc
     4681 ggccaatgca cgcgccacca gcacatccag aggttcatgg cagtagttga cggtacgcaa
     4741 cagcatcggc aggttgacgc ctgcaacgag ccgcgaatgg acgccgtcga tgactttctg
     4801 ggccacattg cagggcgttg cgccgaatac atcggtcagc accagggtgt tgggggtctg
     4861 caactgtgcc agcatggccc gtgcattgag gattgttgtt tcggtaggct cgctgggggg
     4921 tacatccagt gcaatgatgc cgtctgccac atcggggaac acgtgcagca cacccgcacg
     4981 cagggcactt gccaaaggcg catgggcgat gatgagaata ccggtgttca ttcagggggc
     5041 tgtgttgaac ttggattatg gcttctttgc agtgaggaaa tacaggtagg agcccaagca
     5101 gcacaggcca taaacaccca gcgcgccctg gtaggcaaca acttcgctca ttcccgttgt
     5161 acggaagccg tcaatgagca ggcccacccc ccactgcacc gaaaacacgc ccgcaaaaat
     5221 caccaaattg aatgccgaca acgcccggcc agccagttca gcccggaaag ccatccctac
     5281 cgctggttgc acctgcccgc cgaccgtact ggtaacacaa aacaatagcc aggcgaatcc
     5341 agcctccgtc gatattgccc ctccagctat tataatgata gcaaaaatgg cgagcaccac
     5401 cggcattcct ataaccagca accggtccgt ggaatagcct ttgcgcgcca gcgtgggatt
     5461 gagcgagccc caggccagaa aagccaacaa catgctcacg ttgatccaga aaagtcccat
     5521 ggccgcctgt gccggggtgt agcccgccac cttgaccatc caaggcgctg cccacagcgt
     5581 ttgcatggcg accatgccac cgtaattgaa gaagcccaac ggagccatct tgcggaaata
     5641 gggattgcgc cagacctcgc catatcctcg cgatgccgtg gactggctcg aaacgctggt
     5701 tggagcagcg tgccagtgcg gcacctgcag atagatcagc accatggcca aagcaaccat
     5761 cgcgctgagc gcgatgaaca acgggcgcca tcctgccacg ctgacaatcc attgcacagg
     5821 caacgttgag gccaccatac ccagagcgcc caccatcagc atccaggagt tggcccgcat
     5881 gaggttttcc ggcgcaagcc agcggcggta gccagtcagt ggcgccatca gacaggcact
     5941 gaccccgacg ccgcacagaa cacgcgaggc cagcaaccac tggaaactgc tggcctgtgc
     6001 aaaggcgaag gcccccacta cggcaatacc cagaaagccg agaatgacgc gcttcggacc
     6061 gaatcggtcc agccaggtgc ccaaggggag ctgggtgagt gcaaaaccaa agaaatagcc
     6121 gcccgccagc aaacccagat cacgcgcatt gagcgagaac tcggtgacca gcgttggggc
     6181 caatgtcgcg gtgactgccc gtatcagcgt ggagatgaaa taggcaaaag cgaaaaccag
     6241 gaagatggag actgcctgtc gacggggtaa tggtgtcatt gtttactgta gggcgccaaa
     6301 tactttggtc agaatggcac tgccatagcc cacggggttc tgacggatct ttttttcctc
     6361 ttcggaaatc atgaaataca ggccatcgag cgccttgccg gtcacg
//
LOCUS       DAOSJM010000112         6380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11018_length_6380_cov_4.482530, whole genome shotgun sequence.
ACCESSION   DAOSJM010000112 DAOSJM010000000
VERSION     DAOSJM010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11018_length_6380_cov_4.482530"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKX82_05420"
     CDS             complement(<1..419)
                     /locus_tag="PKX82_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF51725.1"
                     /translation="MTDLKALRTEYRAGKALILERMGAPGASARDVRQSMHKLARQAD
                     AMLTQLWTLAGLASPFALLGVGGFGRGELFPYSDVDVLVLLPDGHSPETDAALKGKIE
                     TFIGYCWDVGLEIGSSVRTLSDCIAEAEKDVTVQTALL"
     gene            complement(429..1244)
                     /gene="map"
                     /locus_tag="PKX82_05425"
     CDS             complement(429..1244)
                     /gene="map"
                     /locus_tag="PKX82_05425"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOF51726.1"
                     /translation="MTITYKDAEGIAGMRLAGRLTSEVLDYLTPFIKPGITTNEIDKL
                     AHDYIVDVQKAIPAPLNYTGGGNKPYPKSVCTSINHKVCHGIPNDKPLKKGDIVNVDV
                     TVIKDGWHGDSSRMFMIGDVSIAAKRLCAVTYDAMWHGIKMVKPGIHLGDIGHAIQQF
                     AEGQGFSVVREFCGHGIGKGFHEEPQVLHYGRPGTLEKLVEGMTFTIEPMINAGGKDI
                     KEGPEKDGWSIVTKDRSLSAQWEHTVLVTPTGYEVLTLSSASPPIPAFVPPQA"
     gene            1387..2796
                     /locus_tag="PKX82_05430"
     CDS             1387..2796
                     /locus_tag="PKX82_05430"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HOF51727.1"
                     /translation="MSDSYLDFANSALGSRIAAMLGLPQPVPLERYRADQPVIKGAVL
                     IGTGGNPELLPALSTALHALGATSVAHRSAPQWVALANQAGMMTGRWGVEDKAGEKVK
                     ALVFDATGLEDSTQSDALYQFFHDAARSVLPCGRVVVIGRPPESCAAPRKATVQRALE
                     GLTRSLGKELKRGITAQLVYVAPGAEEQLESTLRFLLSPRSAYVSAQVVHIGLPVGPA
                     QAPADWRRPLEGKKVLVTGASRGIGAAIAEVMARDGAQVICLDVPQAQAGLDEVAARI
                     GGKALALDIGAPEAPAALVAAAQADGGWDVVVHNAGITRDKTIANMKDHLWQAVVNVN
                     LSTQERINEALVAAGALKSGARIVCVSSISGIAGNLGQTNYALSKAGVIGMVQSTAPE
                     LARYGITINAVAPGFIETQMTAAVPFAIREAGRRMNSMGQGGLPVDVAETIAWFASPA
                     SSGLSGNVVRVCGQSLLGA"
     gene            2801..3661
                     /locus_tag="PKX82_05435"
     CDS             2801..3661
                     /locus_tag="PKX82_05435"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOF51728.1"
                     /translation="MKTFEVLREPSLPSVLIGALASSTKRPGQVKKLPPVTLVRPEVV
                     LDGAHIAQYNALCGFTPAQGVPLLYPQLLTFPLVMSYVGSADCPWPALGTVHLANRIE
                     QVRSLKAGDAVRVELQTGTVQAHEKGQVFTLDLRILQEDDVVWSATQTLLRVGVPSPS
                     GKSFASSVTADAPLSHQADFSAPADMGRRYGAVSGDRNPIHLSTPTARLFGFRRAIAH
                     GMWTHARALACVLPQHPLEQASVAVDFKTPLFLPARASLWTHRTAHGALFEVRDAKGQ
                     KPHLRAQLSY"
     gene            3711..5015
                     /locus_tag="PKX82_05440"
     CDS             3711..5015
                     /locus_tag="PKX82_05440"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOF51729.1"
                     /translation="MNTIRRVAILGGNRIPFARSNTVYSKATNQEMLTATLQGLVDRF
                     NLHGERLGDVAAGAVMKHSRDFNLVRECVLSTTLSPETPAFDLQQACGTGLEAAMLVA
                     NKIALGHIDCGIAGGVDTTSDAPIALNEKMRKILLDASRAKTTAQRLTALSRLRPAML
                     VQPALPRNGEPRTGFSMGEHAELMAQEWGVTREAQDELALKSHQNLAKAYDNGFFNDL
                     MTPFQGVTKDNNLRADLSIEKLRSLKPVFDKKVAPNQGTLTAGNSTPLTDGASAVLLA
                     SEEWAAARNLPVLAYLTFSEVAAVDFFGKKEGQNSEGLLMAPAYAVPRMLARAGLTLQ
                     DFDYYEIHEAFAAQVLCTLDAWQDKEFCKDKLGLAKPLGKIDMKKLNVNGSSLAAGHP
                     FAATGGRIVATLAKMLAQKGSGRGLISICAAGGQGVVAIIER"
     gene            5021..>6380
                     /locus_tag="PKX82_05445"
     CDS             5021..>6380
                     /locus_tag="PKX82_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF51730.1"
                     /translation="MLHYKAPLRDMRFLINEVLDYPQHYSTLSNGAEATPEMVDAILE
                     GAATLCEEVLAPLNASGDKEGCHLNDGVVTTPKGFKEAYAQFVAGGWQGLSFPVEYGG
                     QGMPMSLNLFKSEMMGAANWSFNMYPGLSIGCINTLLQYGTEAIKTVYLPRLVAGEWT
                     GTMCLTEPQCGTDLAQVKTRAEPQADGSYALTGTKIFISSGDHDLAENIVHIVLARLP
                     GAPAGTRGISLFVVPKFAVSADGTVGARNGVNTGSLEHKMGISANATAVLNFDGAIGT
                     LIGEPNKGLEAMFTFMNTARIGTAIQGVAHAELSFQGALPYAQERRSMRALSGKKEPE
                     QVADALIWHPDVRRMLLTQKAIAEGGRAMVYGAAQIADKMFNAVQEGDQAKYKKYDDE
                     LGFYTPILKGFLTEMGLEAANLGIQVFGGHGYIGEHGMEQIARDARISTLYEGTTGIQ
                     ALD"
BASE COUNT         1183 a         1987 c         2037 g         1173 t
ORIGIN      
        1 agtagcgccg tctgtaccgt gacgtctttt tcggcttcgg caatgcagtc ggacagggtg
       61 cgcacactgg agccaatttc caggcccaca tcccagcaat agccaataaa ggtctcgatc
      121 tttcccttca gcgccgcgtc ggtttccggg ctgtggccgt ctggcaacag caccagcacg
      181 tccacatctg aatagggaaa caactctccg cgcccgaaac caccgacccc cagaagggca
      241 aaaggtgagg ccagtccggc cagcgtccag agctgggtca gcatggcatc ggcctgccgg
      301 gccagcttgt gcattgactg gcgcacgtcc cgtgctgaag ctccgggagc acccatgcgc
      361 tccagaatga gtgccttgcc ggcacggtat tcggtgcgca gagccttgag gtcggtcatg
      421 gaagaggact aggcctgtgg cgggacaaac gcgggaatcg gcgggctggc gctggacagc
      481 gtgagcactt cgtagccggt aggcgtgacc agaactgtgt gttcccattg ggccgacaac
      541 gaacggtctt tggtgacgat gctccagccg tctttttcgg ggccttcctt gatgtccttg
      601 ccaccggcat tgatcatggg ctcgatggtg aaggtcatgc cttcaaccag cttttccagc
      661 gtgccggggc ggccgtagtg cagcacctgg ggctcttcgt gaaagccctt gccgatgccg
      721 tggccacaga actcgcgcac cacgctgaaa ccctggcctt cggcaaactg ctggatggcg
      781 tggcctatgt cgcccagatg gatgccgggt ttgaccatct tgatgccatg ccacatggcg
      841 tcgtaggtca ccgcgcacag ccgtttggct gcgatggaga catcgccaat catgaacatg
      901 cggctggaat cgccatgcca gccgtctttg atcacggtga cgtccacgtt gacgatgtcg
      961 cccttcttca agggcttgtc attgggaatg ccatggcaga ccttgtggtt gatggaggtg
     1021 cagactgact tggggtaggg cttgttgcca ccgcccgtgt agttcagcgg ggcaggaatg
     1081 gccttttgca cgtccacgat gtagtcgtgg gccagcttgt caatttcgtt ggtggtgatg
     1141 ccgggtttga taaacggcgt cagatagtcc agaacttcgg aggtcagccg gccagccagg
     1201 cgcatgcctg caatgccttc ggcgtctttg taggtgatag tcatggggcg agattatccc
     1261 agaggctccc gggcgacggc ggcgtaggcc ttttccgggc caagacggtg cggttaggca
     1321 cctcgcccta gtgcaactga ccaatgggtc gttaacatcg cggcaatttg caacaggagc
     1381 actgggatgt ctgattcgta tctggatttt gccaattcgg cattgggttc acgcatagcc
     1441 gcgatgctgg gtctgcccca gccggtgcca ctggagcgct accgtgcgga ccagcccgtg
     1501 atcaaggggg ctgtactgat tggcactggt ggcaaccccg agttgttgcc cgcgcttagc
     1561 accgcattgc atgccctggg cgcgaccagc gtggcccacc gcagcgcccc ccagtgggtg
     1621 gcattggcca accaggccgg catgatgacc gggcgctggg gcgtggaaga caaggcgggt
     1681 gagaaggtca aggccctggt gtttgacgcc accggcctgg aagacagcac gcaatccgat
     1741 gcgctgtacc agtttttcca cgacgccgcg cgctcggtgc tgccttgcgg ccgtgtggtc
     1801 gtgattggcc gcccgcctga atcgtgcgcc gcgccacgca aggccacggt acagcgcgca
     1861 ctggaaggcc tgacccgctc cctgggcaaa gaactcaagc gcggcattac cgcccaactg
     1921 gtgtacgtgg cgcccggtgc agaggaacag ctggaaagca cgctgcgctt cctgctgtcg
     1981 ccacgctcgg cttatgtgtc tgctcaggtg gtacacattg gcctgccggt ggggcctgca
     2041 caagcacctg ccgactggcg ccgtccgctg gagggcaaga aggtactggt gaccggcgca
     2101 tcccgcggca ttggcgctgc catcgccgaa gtcatggcgc gcgacggcgc ccaggtgatc
     2161 tgcctggacg tgccccaggc acaggctggc ctcgacgaag tggccgcccg gatcggtggc
     2221 aaggcgctgg cgctggacat tggcgcgccg gaagcccccg cagcgctggt cgccgcggca
     2281 caggccgacg gtggatggga tgtggtggtc cataacgcag gcataacccg cgacaaaacc
     2341 atcgccaata tgaaagacca cctgtggcag gccgtggtca acgtcaacct gtccactcag
     2401 gagcgcatca acgaagcact ggtggccgcg ggagccctca agtcaggtgc gcgcatcgtc
     2461 tgcgtgtcgt ccatttccgg cattgccggc aatctgggac aaaccaatta cgcgttgtcc
     2521 aaagccggcg tcatcggcat ggtgcaaagc accgcgcccg aattggcacg ctatggcatc
     2581 accatcaacg ccgtggctcc cggctttatc gagacgcaga tgactgcagc cgtgccgttt
     2641 gccatccgcg aggcgggtcg ccgcatgaac tccatggggc agggcggtct gccggtggac
     2701 gtggccgaga ccattgcctg gtttgccagc ccggcctcca gcggtctgtc gggcaatgtg
     2761 gtgcgcgtgt gcggacaaag cctgctcggg gcctgaagcc atgaagacct tcgaagttct
     2821 gcgcgagccg tcgctgccca gcgtgttgat cggggccctg gcctccagca ccaagcgccc
     2881 cggccaggtc aaaaagctgc cccccgtcac cctggtgcgc cccgaagtgg tgctggatgg
     2941 tgcgcacatt gcccagtaca acgcgctgtg tggtttcacg cccgcgcagg gcgtgccgct
     3001 gctgtacccc cagttgctca cgtttccgct ggtgatgtcc tatgtggggt ccgccgattg
     3061 tccctggccc gcgctgggta cggtgcatct ggccaaccgt atcgagcagg ttcgcagcct
     3121 gaaagcgggc gatgcagtgc gtgtggaatt gcagaccggc acggtgcagg ctcatgaaaa
     3181 aggccaggta ttcaccttgg acttgcgcat cctgcaagag gacgatgtcg tctggagcgc
     3241 cacccagacg ctgttgcgcg tgggcgtgcc gtcgcccagt ggcaagtcct ttgccagtag
     3301 cgtgacggcc gatgcaccgc tgtcccacca ggcggatttt tctgcgcctg ccgatatggg
     3361 ccgccgctat ggcgccgtgt ccggcgaccg caacccgatc cacctgagta cgcccacggc
     3421 gcgcctgttc ggtttccgcc gggccattgc gcatggcatg tggacccatg cccgggcttt
     3481 ggcctgcgtg ctgccgcagc atccgctgga gcaggcgagt gtggcggtgg acttcaagac
     3541 gccgctgttt ttgccggccc gtgcctcgct gtggacccac cgcactgcgc atggcgcgct
     3601 gttcgaggtc cgtgatgcca aggggcaaaa gccccacctg cgcgcgcagc tgagctactg
     3661 atcgtccgag cgcctgctac tcaaccccaa tttatagaaa gcttattgcc atgaacacca
     3721 tccgtcgcgt cgccatcctg ggcggcaacc gcattccgtt cgcccgctcc aacaccgtgt
     3781 actccaaggc caccaaccag gaaatgctga ccgccaccct gcagggtctg gtggaccgct
     3841 tcaacctgca tggcgagcgc ctgggcgatg tggccgctgg tgcggtgatg aaacattcgc
     3901 gcgacttcaa cctggtgcgc gaatgtgtgc tgtcgaccac gctgtcgcca gaaacacctg
     3961 cattcgactt gcagcaggcc tgtggcaccg gcctggaagc ggccatgctg gtggccaaca
     4021 agattgcgct ggggcacatt gactgcggca ttgccggcgg cgtggacacc acgtccgacg
     4081 cgccgattgc cctgaacgaa aaaatgcgca agattttgct ggacgcctcg cgcgccaaga
     4141 ccacggcgca acgcctgacc gcactgtcgc gcctgcgccc cgcgatgctg gtgcaacccg
     4201 cgctgccgcg caacggcgaa ccgcgcaccg gtttctccat gggtgaacac gccgagctga
     4261 tggcgcagga atggggcgtg acccgcgaag cgcaggacga actggcgctc aagagccacc
     4321 agaacctggc caaggcctat gacaacggct ttttcaatga cctgatgacc cctttccagg
     4381 gcgtcaccaa agacaacaac ctgcgcgccg acctgagcat cgagaagttg cgcagcctca
     4441 aacccgtgtt tgacaagaag gtcgcgccca accagggcac gctgaccgcg ggcaactcca
     4501 cgccgctgac cgacggtgca tccgccgtgt tgctggccag cgaggagtgg gctgcggcgc
     4561 gcaatctgcc ggtgctggct tacctgacgt tcagcgaagt ggcggctgtc gatttcttcg
     4621 gcaagaagga aggccagaac agcgagggct tattgatggc tccggcctat gccgtgccgc
     4681 gcatgctggc gcgcgcaggc ctcacgctgc aggactttga ctactacgaa atccatgaag
     4741 cctttgcggc gcaggtgctg tgcacgctgg acgcctggca ggacaaggag ttctgcaaag
     4801 acaagctggg cctggccaaa ccgctgggca agatcgacat gaaaaagctc aacgtcaatg
     4861 gtagttcgct ggcagcgggt cacccctttg ccgccaccgg cgggcgcatt gtggccacgc
     4921 tggccaagat gctggcgcaa aagggctcgg gccgtggcct gatttcgatt tgcgccgctg
     4981 gtggccaggg tgtcgtcgcc atcattgaaa ggtaagagtc atgttgcact acaaagctcc
     5041 tctgcgcgat atgcgcttcc tgatcaacga agtgctcgac tatccccagc actacagcac
     5101 gctgtcgaat ggcgccgagg ccacaccgga gatggtcgat gccatcctgg aaggtgcagc
     5161 aacgctgtgt gaagaagtgc tggcaccgtt gaatgcttcg ggcgacaagg aaggctgcca
     5221 tttgaacgac ggcgtggtga ccacgcccaa gggcttcaag gaagcctatg cccagtttgt
     5281 ggctggtggc tggcagggtc tgtccttccc cgtggaatat ggcggccagg gcatgcccat
     5341 gtcgctgaac ctgttcaagt cggaaatgat gggcgcggcc aactggtcgt tcaacatgta
     5401 ccccggcctg agcatcggct gcatcaacac gctgctgcaa tacggaaccg aggccatcaa
     5461 gacggtgtac ctgccgcggc tggtggcggg tgagtggacc ggcaccatgt gcctgaccga
     5521 accccagtgc ggcaccgacc tggcccaggt caagacccgc gccgagccac aggccgatgg
     5581 cagctacgca ctgaccggga ccaagatttt catctcgtcc ggtgaccacg acctggcgga
     5641 aaacatcgtc cacatcgtgc tggcccgcct acctggcgca ccggctggca cgcgcggcat
     5701 ttcgctgttt gttgtcccga agtttgcggt aagtgcggac ggcaccgtcg gcgcgcgcaa
     5761 tggcgtcaac accggctcgc tggagcacaa gatgggcatc agcgccaacg ccaccgccgt
     5821 gctcaacttt gacggtgcca ttggtaccct gattggcgag cccaacaagg ggctggaagc
     5881 catgttcacc ttcatgaaca cggcccgcat tggcaccgcc atccaggggg tggcccatgc
     5941 cgagctgtcg ttccagggcg cgttgccgta tgcgcaggag cgccgttcca tgcgcgcact
     6001 ctcaggcaag aaagagcccg agcaagtggc tgatgcgctg atctggcacc ccgatgtgcg
     6061 ccgcatgctg ctgacgcaaa aagccattgc tgagggcggc cgggccatgg tctatggcgc
     6121 agcccagatc gcagacaaga tgttcaacgc ggtgcaggaa ggcgaccagg ccaagtacaa
     6181 gaagtacgac gacgaactgg ggttctacac ccccatcctc aagggttttc tgaccgaaat
     6241 gggtctggaa gcggccaacc tgggcatcca ggtgtttggc ggccatggct acatcggtga
     6301 acacggcatg gagcagatcg cgcgcgacgc ccgcatctcc acgctgtacg aaggtacgac
     6361 cggcatccag gcgctggacc
//
LOCUS       DAOSJM010000113         6380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11019_length_6380_cov_4.055020, whole genome shotgun sequence.
ACCESSION   DAOSJM010000113 DAOSJM010000000
VERSION     DAOSJM010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11019_length_6380_cov_4.055020"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKX82_05450"
     CDS             complement(<1..403)
                     /locus_tag="PKX82_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51731.1"
                     /translation="MNEIQAKVHAGKLTWIRTLTAVASVAALAIFAPVVWLAASGGVG
                     LLVLGGILAIGFAAVQTLPLMGQKLENTLLSARKAEARRNPIEQLQNEVLRRAERLRS
                     FRKALVTVGGQIESIRQMIESSQTKFTGPVLA"
     gene            complement(536..1597)
                     /locus_tag="PKX82_05455"
     CDS             complement(536..1597)
                     /locus_tag="PKX82_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOF51732.1"
                     /translation="MTVHHHSGHEINAIRQCSGLKGILKKCVAVGAAAVSVFSMGMQP
                     ALAQAPIVIQFSHVVTADTAKGKAALKFKELAETRTAGKVRIEVYPNSQLYKDREEMD
                     ALKLGAVQMLAPSLSKLGGDFEVFDLPFLFQDHAAYRKVVDGNMGADLLRSLELRGIK
                     GLAYWDNGFKVFTANRPLQSVQDFKGLRIRVQASRTLVRQMNLLGSEPSVSPLINVFE
                     ALRNGRLDGQENTPVNVVSQRLHEVQSHLTVSNHGYLAYAVIVNKTFWDKLPPAIRDT
                     LEGALREATAYANSIAEAENTKALERIKVSGKLSVYTPSEQELGQWRATLDPVAMASR
                     SGISPELVTAIRTATGPPR"
     gene            1733..3700
                     /locus_tag="PKX82_05460"
     CDS             1733..3700
                     /locus_tag="PKX82_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF51733.1"
                     /translation="MTVSTAPQPFQPTAPASWINRQLWLLPFLLAVFFVAMIAYWAEA
                     NDAQEQSAYQNTLMADIQSVEMQLMGRQDIERTKLREVAHKLVGSPSVGDVALQSLPE
                     VAAGMDRLWNRLVWIDADNQVIARAHREVLAQTASTDSLKIQSTGQAEHFVVPVTSID
                     GKPAGQLLARYEITDLLQSTDLAWLNRRYQVDFVSELGEVIATTESPIKVPRGSRYEK
                     PLASFKDTTLRLTQFEAHASWRTNARTMALLGGLLLLGGAASQLLRREMVRVKRAVMA
                     SQTEAAWRQSMEDSALVGLRARDPDGRILYVNKTLCTMVGYSREELVGLIPPLPFWPP
                     EAVDGMMARNMNTLAGESPAEGFETRWRHRDGHILDVMIFEAPLVNSHGVRIGWMGSI
                     VDISERKALEEKERHHVEAMAQHARLNDMGLIASELAHELNQPLTSIASYSAGLEIAL
                     KKRLPDESDLLGAVDAVHRNAKKAGDIVNWIRRQSSRSESLRVACNLNVLVAESLKQR
                     KRQIERNRIQMHLNLAPDLPMVTVDRIGVEQVIANLVRNAADALEAQNGVRSIWIDTR
                     LSEDASTRNATVEVLVRDNGPGLQGRTIDMLCSTFYSTKTNGMGLGLGICRAIVESHG
                     GRLSAGDAPTGGAEISFTIPVSQAIQEKEFQ"
     gene            3697..4344
                     /locus_tag="PKX82_05465"
     CDS             3697..4344
                     /locus_tag="PKX82_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF51734.1"
                     /translation="MTEQATIYLCDDDEDVRGGLSFLLRHSGFDVRAFASGKELLECI
                     EVEPKPLRAIFVLDLDMPPMDGDVVHDQLIEMGYSKRNPVVFLSGRGTIARAVSAVNK
                     GALSFVEKPYTNDTLLPLLHRAVALEEQWTAEAKRCDFLRYMWDSLSAQQRKVALLVA
                     AGDLNKTVATKLEIVERTVEMHRAKAFEKLGVDSPAALATTVAAMKSCGIEMHTE"
     gene            complement(4365..4721)
                     /locus_tag="PKX82_05470"
     CDS             complement(4365..4721)
                     /locus_tag="PKX82_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HOF51735.1"
                     /translation="MNSTLPACPQCGLENTYPDATNYICPDCGFEWPMEEATESADAD
                     ASDGIVRDSNGNPLADGDSVILIKDLKVKGSSTVLKKGTKVKSIRLPQGAGDHEVDCK
                     MDGGSFMLKACFLKKA"
     gene            complement(4746..5528)
                     /locus_tag="PKX82_05475"
     CDS             complement(4746..5528)
                     /locus_tag="PKX82_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF51736.1"
                     /translation="MQATAYRFTPTAWNTLVGQEEDALLTTEERRNINAGAWFSSLSP
                     TLRHDILRSTRVKRYAHGELIAARDVSAPVWLACAQGVLRVGYTTATGKAYTLEYVEP
                     GQWLGEFLLMGDAPHTHDIYAHGATTTLQIQRSAFMEIFATHVEFREALMRRQREDTR
                     LLYEKVDDLKTLGLGARLTKTLLELAQTYGVTVAGRVHITLRLVQKDLAQLLGASRQQ
                     VNQQLKRLEEGNVIRISVGGVVIQDMDALRRHVQRAQLAYED"
     gene            5662..6375
                     /locus_tag="PKX82_05480"
     CDS             5662..6375
                     /locus_tag="PKX82_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOF51737.1"
                     /translation="MPTTAQALDLPAKFSFADFRRATPLDETSLWGVIFDRNAHRISV
                     KRRDTALDNLQRIFAATFRLANDSGFQAMTLRDLCRETGMSMGGLYSYISSKELLSAM
                     IEDVIRYLCEMLAEWFSAIPDPLDRVEAVLRGHLCLSEMLQPWFFFMYLETRTLPPEQ
                     RRESMASELGIEKHIADTLAAQGSLSSSESQLLASHALAMVQDWHLKRWKYSAAGVNV
                     DTFADSVVAVVRARVAVRG"
BASE COUNT         1256 a         1823 c         1958 g         1343 t
ORIGIN      
        1 gggccagcac cgggccggtg aactttgtct gactgctttc gatcatttgc cggatcgact
       61 caatctggcc tcccactgtt accagtgctt tgcggaatga acgcagccgt tcggcacggc
      121 gcagcacttc gttttgcagc tgttcaatcg ggttgcgtct ggcttcggcc ttgcgtgccg
      181 aaagcagtgt gttttccagc ttctgcccca tcagtggcag cgtctgaacg gccgcgaagc
      241 cgatggcgag aatgcctccc aacaccagca gacctacacc gcctgaggca gctagccaga
      301 cgaccggagc aaagatggcc agtgcggcca cacttgcgac cgccgtgagc gtgcgtatcc
      361 aggtcaattt gccggcatgg accttcgctt ggatctcgtt catgtgcttc tcctttgata
      421 cgaacgtggg tgggttggtg tggaatttcg gacgcagctc ttccccgcag gcctggggcc
      481 tgcaaatcaa atggggtggg aacgacgtgt cggcggtcag ccgatgctga aggccttagc
      541 gcggtggacc cgtggcagtc cggattgctg tgaccagctc gggggaaatt ccggaccggg
      601 aagccatagc gacaggatcc agcgttgcgc gccactgacc gagctcctgt tcgctcgggg
      661 tgtagacgct gagcttgcca ctcactttga tgcgctccag tgccttggta ttctcagctt
      721 cagcaatact gttggcatac gcagtagcct cgcgcagagc gccttccagt gtgtcgcgaa
      781 tggctggtgg aagcttgtcc cagaaggtct tgttcacaat cacggcatag gccaggtatc
      841 cgtgattgga gacagtgagg tggctttgaa cttcatggag gcgctgagag accacgttga
      901 cgggcgtgtt ctcctgtccg tccaggcgtc cgttgcgcag tgcctcgaag acgttgatca
      961 gaggcgagac cgatggctcc gagccgagca ggttcatctg ccggaccagt gttctggacg
     1021 cttgtacccg gatcctcagg cctttgaaat cctgcaccga ttgcaagggg cggttggcag
     1081 taaatacctt gaacccgttg tcccaataag ccaagccctt gatgccccgc agttctagcg
     1141 agcgcagcag atcggcaccc atgttgccat ccaccacctt tcggtacgcc gcatggtcct
     1201 ggaaaagaaa aggaagatca aagacctcga agtcgccgcc aagcttggac aacgaaggtg
     1261 ccagcatctg cactgcaccg agcttcaagg cgtccatctc ttcgcgatcc ttataaagct
     1321 ggctgttggg ataaacctcg atacgcacct tgccggcggt gcgggtctcg gccagctcct
     1381 tgaatttcag ggccgccttg cccttggccg tatcggccgt taccacatgg ctgaattgaa
     1441 tgacgatcgg tgcctgggcg agggccggtt gcatacccat tgaaaagaca gaaacagcag
     1501 ctgcgccgac cgcaacacac ttcttaagga tgcccttcaa tccggaacat tgcctgatgg
     1561 catttatctc gtgtcctgag tggtggtgta ctgtcatttg cttctcccct ggatgactga
     1621 agtctatgcg ccggtgaatg acccaggaag tgtggtgatc cacagatgtg ggttcccaca
     1681 ccttggccgt tccggaggca tattgcagca gacttgaccc tagaatttgc cgatgaccgt
     1741 atccaccgct ccccagccgt ttcagccgac tgctcccgcg tcgtggatca acagacagct
     1801 ctggctgcta ccgttcctgc tggctgtctt ttttgttgcc atgatcgcct attgggcaga
     1861 agccaacgat gcccaagagc agtctgccta ccagaacacc ctcatggcag acatacagag
     1921 tgtcgaaatg cagctcatgg gtcgccagga catcgagcgc accaagttgc gggaagtagc
     1981 gcacaagctt gttgggtccc cttccgtggg ggacgtcgcg cttcaatcgt tgccggaggt
     2041 cgctgcggga atggaccgcc tgtggaatcg tctggtgtgg atcgacgcgg acaaccaggt
     2101 cattgcacgt gcccatcgag aagtcttggc acagactgcc tcaaccgaca gtctgaagat
     2161 ccagtcgacc gggcaggctg agcattttgt cgttccagtg acttcgatcg atggcaagcc
     2221 ggctgggcag ctgctcgcac gatacgaaat caccgatctg ctgcaaagca ccgatctggc
     2281 ctggttgaac cggcggtacc aggtggactt tgtttcggag cttggagagg tgatagcaac
     2341 cactgaaagc cccattaaag tgccgcgcgg aagcaggtac gaaaagccgt tggcctcctt
     2401 caaggacact accttgcggc tgacccagtt tgaagcgcat gcatcgtgga gaaccaacgc
     2461 ccgaacaatg gcattgctgg gaggtctcct gcttctgggt ggtgccgctt cgcaactgct
     2521 acgcagggag atggtccgtg taaaacgcgc cgtgatggcg tcgcagaccg aagcggcgtg
     2581 gcgtcagtcc atggaagatt ccgcactggt ggggctgagg gcccgtgacc cggacggccg
     2641 aatcctgtat gtgaacaaaa ccctctgcac gatggtgggc tattcgcgtg aagagttggt
     2701 cgggctcatt ccgcccttgc cattttggcc gcccgaggct gtcgacggca tgatggcacg
     2761 caacatgaac acccttgcag gtgaatctcc ggcagaaggt tttgagacac ggtggagaca
     2821 ccgcgacggt cacatactgg atgtcatgat cttcgaagca ccgttggtca attcgcacgg
     2881 tgtgcggatt ggctggatgg ggtctatcgt ggacatcagc gagcgcaaag ccctggaaga
     2941 aaaggagcgc caccatgtcg aagccatggc acagcacgcc cggctgaacg acatggggct
     3001 gattgcatcg gaattggccc atgagctcaa ccagccactg acctccattg ccagctacag
     3061 cgccggactt gaaattgccc tgaaaaaacg cctgcctgat gaaagcgatt tattgggtgc
     3121 tgtggatgcg gtgcatcgca atgcgaagaa ggctggagac attgtgaatt ggatacgccg
     3181 ccagtcttcc cgttcagagt ctctgcgcgt ggcatgcaat ttgaatgtgc ttgtggcaga
     3241 gtccctgaaa cagcgcaaac gtcagatcga acgcaaccgg attcagatgc atctgaatct
     3301 tgcaccggat ctgcccatgg tcacggtcga ccggatcggc gtcgagcagg tcattgccaa
     3361 tctggtgaga aatgcggctg acgctctgga ggcacaaaac ggagtgcgct ccatatggat
     3421 tgacacccgt ttgagtgagg atgcgtctac gcgtaacgcc actgtagagg ttctcgtacg
     3481 ggacaatggc cccgggttgc aggggcgaac tatcgacatg ctgtgctcca ctttctactc
     3541 tacaaaaacc aacggtatgg gcttggggct cggcatatgc cgagccatcg tggagtccca
     3601 tggcggcagg ctcagcgctg gggacgcgcc caccggcggg gctgaaatca gtttcacgat
     3661 tcccgtctcc caagccattc aagagaaaga attccaatga cagagcaagc aaccatctat
     3721 ctgtgtgatg acgatgagga tgtccgtggt ggtctgtcat tcctgttgcg ccattccggc
     3781 tttgacgtgc gcgcttttgc cagtggaaag gaattgcttg agtgcattga agttgaaccc
     3841 aagccattgc gtgccatttt tgtcctggat ctggacatgc cgcccatgga tggtgatgtg
     3901 gtgcatgacc agcttattga aatgggctac tcgaagcgca accccgtcgt gttcctgagc
     3961 ggcaggggca cgattgccag ggcggtcagc gcggttaaca aaggagcact cagctttgta
     4021 gaaaagccct acacgaatga cacgcttctg ccgcttcttc accgggccgt ggcgctggag
     4081 gagcaatgga ctgcagaggc caagcgctgc gatttcctcc ggtatatgtg ggacagtctc
     4141 agtgcgcagc agcgtaaggt tgcgctgctg gttgccgcgg gcgatttgaa caagaccgtt
     4201 gcaaccaagc tggagattgt tgaacgcacc gtggaaatgc accgggccaa ggcatttgaa
     4261 aaactggggg tcgattcacc cgctgcactt gccaccacgg ttgctgccat gaaatcctgc
     4321 gggatcgaaa tgcacacaga atgacaaagt agccagccct gccatcaagc cttcttaaga
     4381 aaacaggcct tgagcatgaa gctgccgccg tccatcttgc agtccacctc gtggtcgccc
     4441 gcgccctggg gcaggcggat gctcttgacc ttggtgccct tcttgagcac ggtggaggag
     4501 cctttgactt tcaggtcttt gatcaggatg acggagtcac cgtcggccag cgggttgccg
     4561 ttggaatcgc gcacgatgcc gtcagaagcg tcggcgtctg cagactccgt ggcttcctcc
     4621 atgggccatt caaacccgca gtcggggcag atgtagttgg tggcgtcggg gtaggtgttt
     4681 tccaggccgc attgcgggca ggcaggtaag gtgctgttca tgccccgatt atccctgcca
     4741 ggatttcagt cctcgtaggc cagttgcgcc cgctggacat ggcgtcggag cgcatccatg
     4801 tcctgaatga ccacgccacc cacgctgatg cggatcacgt tgccctcttc caggcgcttg
     4861 agctgctggt tgacctgctg gcgcgatgca cccagcaatt gcgccagatc cttctgcacc
     4921 agccgcagcg tgatgtgcac acgtccggcc acggtgacgc cgtaggtctg cgccagctcc
     4981 agcagcgtct tggtcagccg ggcgcccagc cccagggtct tgaggtcgtc caccttttcg
     5041 tacagcagcc gggtgtcttc gcgctggcgt cgcattaatg cttcgcggaa ttccacatgg
     5101 gtggcgaaga tctccatgaa ggcactgcgc tggatctgca gcgtggtcgt ggcgccgtgg
     5161 gcatagatgt cgtgcgtatg cggcgcgtcg cccatcagca ggaattcgcc cagccattgg
     5221 ccaggttcca cgtattcgag ggtgtaggcc ttgccggtgg ccgtggtgta gcccacgcgc
     5281 agcacgccct gggcgcaggc cagccagacc ggggcgctga cgtcccgcgc agcgatcaac
     5341 tcgccatgcg cgtagcgctt gacccgcgtg ctgcgcagga tgtcgtggcg cagcgtgggc
     5401 gacagggagg aaaaccaggc gcccgcattg atgttgcgcc gctcctcggt ggtgagcagt
     5461 gcgtcttctt cctggccgac gagcgtgttc cacgccgtgg gggtgaaacg gtaggctgtt
     5521 gcctgcatgg cggtagattg cattcaaata cccaacggag ctgtatgttt agaaaacgag
     5581 cgctcgctcg ttattttttc ggatggtacg atgccggtca tcggctgtca atcagcgtac
     5641 ggaattcggt ctcactacgt catgcccacc actgcgcaag cccttgattt gccagcgaaa
     5701 ttcagctttg ctgacttccg ccgggcgacg ccgctggacg aaaccagcct gtggggggtg
     5761 atctttgacc gcaatgccca tcgcatcagc gtcaagcggc gggacacggc gctggacaac
     5821 ctgcagcgca tctttgctgc caccttccgg ctggccaatg acagcggctt tcaggccatg
     5881 acgctgcgcg acctgtgccg cgaaaccggc atgtccatgg gcgggctgta cagctacatc
     5941 tccagcaagg aactgctctc ggccatgatc gaggatgtga tccgctacct gtgcgagatg
     6001 ctggccgaat ggttcagcgc catcccggac ccgctggacc gggtggaagc ggtgctgcgg
     6061 ggccacctgt gcctgtcgga aatgctgcag ccctggttct tcttcatgta cctggagacg
     6121 cgcacgctgc cgccggagca gcggcgcgaa tccatggcgt ccgagctggg gatcgagaag
     6181 cacattgccg acaccctggc cgcgcagggc agcctgagca gcagcgagag ccagctactg
     6241 gcctcccacg cactggccat ggtgcaggac tggcacctca agcgctggaa gtacagcgcg
     6301 gcgggcgtca atgtggatac ctttgccgac agtgtggtgg cggtggtgcg ggcgcgggta
     6361 gcggtccggg gataagcttg
//
LOCUS       DAOSJM010000114         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11132_length_6331_cov_4.651211, whole genome shotgun sequence.
ACCESSION   DAOSJM010000114 DAOSJM010000000
VERSION     DAOSJM010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11132_length_6331_cov_4.651211"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /gene="xseA"
                     /locus_tag="PKX82_05485"
     CDS             <1..961
                     /gene="xseA"
                     /locus_tag="PKX82_05485"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOF51738.1"
                     /translation="EQFLILKEKLDREGLFDPQRKRPLTAMPRAIGVVTSLGAAALHD
                     VVTALQRRVPHIPVVLAPASVQGEGAPRELVKALESLFTLARDEGSRSPVDTILLVRG
                     GGSMEDLWAFNDEALARVIARSPVPVVCGVGHETDFTIADFVADLRAPTPTAAAELVA
                     MPTQVWLDEVQRLDERLQEALTRRTDREAQRLDQVTARMGRPSQLAHRSRMHLASLEQ
                     GLSHAMQIRLRTCRSTLESMGGAVPRTMQRTLEIEREQLARAALRLGALDPSLVLRRG
                     YAWLADGKNRPITSITQTQVGQQVVATLADGAVDMTVQHRHAN"
     gene            1005..1586
                     /locus_tag="PKX82_05490"
     CDS             1005..1586
                     /locus_tag="PKX82_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOF51739.1"
                     /translation="MEHTLPPLPYAIDALAPAYSKETLEFHHGKHHNAYVVNLNNLQK
                     GTEFENMSLEEIIKKSSGGVYNNSAQIWNHTFFWNCMKPAGGGEPSGALAAAINAKWG
                     SYAAFKEAFVKSAVGNFGSGWTWLVRKPDGSVDIVNMGAAGTPLTTADKALLTVDVWE
                     HAYYIDYRNMRPKFVETFLDKLVNWSFAEANFT"
     gene            1830..4067
                     /locus_tag="PKX82_05495"
     CDS             1830..4067
                     /locus_tag="PKX82_05495"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOF51740.1"
                     /translation="MSNKQPTIIYTLTDEAPRLATASFLPIVRAFASAAGVDVLESDI
                     SVAARVLATFPECLTEAQRVTDNLAELGKLTLLPDTNIIKLPNISASVGQLVTCIKEL
                     QSKGYAVPDYPEAPKTEEEKAIRARYAKCIGSAVNPVLREGNSDRRAPRAVKEYARKN
                     PHSMAEWSQASRSHVSHMHHGDFYHGEKSMTLDKARDVKMELLTKSGKTIVLKPKVSL
                     LDREVIDSMFMSKKALLEFYEKEIEDARKTGVMFSLHVKATMMKVSHPIVFGHCVKIF
                     YKDAFEKHAKLFEELGVNVNNGMANLYDKIATLPQSTQDEIKRDLHACHEHRPELAMV
                     DSAKGITNFHSPNDVIVDASMPAMIRNGGKMWDANGRLKDVKAVMPESTFARIYQEMI
                     NFCKWHGAFDPKTMGTVPNVGLMAQQAEEYGSHDKTFEITEDGVANITDLATGEVLMS
                     QNVETGDIWRMCQVKDAAIRDWVKLAVNRARNSGMPVVFWLDSYRPHEAQLITKVKMY
                     LHEHNTAGLDIQIMSQVRAMRYTLERVIRGEDTISATGNILRDYLTDLFPIMELGTSA
                     KMLSIVPLMAGGGMYETGAGGSAPKHVQQLVEENHLRWDSLGEFLALAVSLEDLGLKT
                     GNAQAKILAKTLDAATGKLLDNNKNPSPKTGQLDNRGSQYYLAMYWAQELAAQSDDRE
                     LAARFTSLAKQLTDSEQAVVAELAAVQGKPADIGGYYLPDLGKLDAVMRPSKTLNTVL
                     ASLKS"
     gene            complement(4136..4594)
                     /locus_tag="PKX82_05500"
     CDS             complement(4136..4594)
                     /locus_tag="PKX82_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOF51741.1"
                     /translation="MKSFLTRLLPLLMVVAGSALAQDSPQLNLPRVKLGAGIHQIDAQ
                     VAQTQEQQAIGLMFRREMPPHEGMVFVFSQPSKLCFWMKNTLLPLTAAFVADDGTIVN
                     LADMKPQTTDSHCAAKPVRFVLEMNQGWFAKRSIKAGAKLTGPLFDGRSN"
     gene            4741..5994
                     /gene="icd"
                     /locus_tag="PKX82_05505"
     CDS             4741..5994
                     /gene="icd"
                     /locus_tag="PKX82_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOF51742.1"
                     /translation="MYQHIKVPAQGQKITVNADMTLNVPDQPIIPYIEGDGTGLDITP
                     VMLKVVDAAVAKAYGGARKIHWMEVYAGEKSTRIYGPDVWLPQETLDVVREYVVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPIQYFKGVPSPLKEPEKTNMVIFRENSEDI
                     YAGIEFEAESDKAKKLIKFLQDEFGVTKIRFPNTSGIGIKPVSREGTERLVRKAIQYA
                     IDNDKPNVTIVHKGNIMKFTEGGFRDWSYALAQREFGAELIDGGPWCKFKNPRTGKYI
                     TIKDSIADAFLQQILLRPSEYSVVATLNLNGDYISDALAAQVGGIGIAPGANLSDTVA
                     MFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWKEAADLIIRSIERSIASKKVTY
                     DFARLMDGASQVSCSGFGQVMIDNM"
     gene            6158..>6331
                     /locus_tag="PKX82_05510"
     CDS             6158..>6331
                     /locus_tag="PKX82_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOF51743.1"
                     /translation="MASNKPTQPPVPPVQVPERDDSNAVVLERRTQRVGPPHMYQVVM
                     LNDDYTPIEFVVVV"
BASE COUNT         1427 a         1716 c         1845 g         1343 t
ORIGIN      
        1 tgaacaattc ctcatcctga aggaaaaact ggatcgggaa gggctgtttg accctcagcg
       61 caagcgccca ttgacggcta tgccgcgggc cattggtgtc gtgacttcgc tcggcgcggc
      121 agcattgcac gatgtggtca cggccctgca gcgccgtgtg ccgcatattc ccgtggtgct
      181 ggcgccggcg tccgtgcagg gagaaggcgc accacgtgaa ctggtcaagg cgctggagtc
      241 cctcttcact cttgcaaggg acgagggtag ccggtcgcca gtggatacca ttttgctggt
      301 gcgtggcggc ggatccatgg aggacctatg ggcgttcaat gatgaagctt tggcccgcgt
      361 gattgcgcgc agtccggtac ctgtggtgtg cggcgtgggg cacgagaccg atttcacgat
      421 tgccgatttt gtggccgatc tgcgggcacc cacacctacc gcggcggcag agctggtggc
      481 catgcccacc caggtctggc tggacgaggt acagagactg gatgaacgtt tgcaggaagc
      541 cctgacacgg cggacagatc gcgaagccca gcggctcgat caggtgactg cccgcatggg
      601 caggccttcg cagctggcgc accgcagccg aatgcacctg gcgtctctgg agcagggtct
      661 gtcacatgca atgcaaatcc ggctgcgcac ttgccgttct acgttggagt ccatgggagg
      721 tgcagtaccc cggaccatgc agcgcacgct ggaaatcgag cgcgagcagc ttgcacgtgc
      781 agcgttgcgt cttggagcac tggacccgag tctggttttg cgccggggct acgcctggtt
      841 ggcagacggg aagaatcgac ctatcaccag catcacacaa acccaggtgg ggcaacaggt
      901 ggtcgccacc cttgctgacg gtgcggttga tatgactgtg cagcacagac acgccaatta
      961 gtttctacaa tcgccgtttt cttgaccaca aaccagagga attcatggaa cataccttgc
     1021 cccccctgcc ttacgccatc gacgcgctgg ctccagccta ttctaaagag accctggagt
     1081 tccaccacgg caagcaccac aatgcctatg tggtcaactt gaacaacctg cagaagggga
     1141 cagagttcga gaacatgtcc ctggaagaaa tcatcaagaa atccagcggc ggcgtttaca
     1201 acaattccgc acaaatctgg aaccacactt tcttctggaa ctgcatgaag ccagctggcg
     1261 gtggtgagcc ttccggcgcc ttggccgcag ccatcaatgc caagtggggc agctatgccg
     1321 ctttcaagga agctttcgtg aagtccgcgg tgggtaattt tggctctggc tggacatggc
     1381 tggtcaggaa gcctgatggc agcgtggata tcgtcaacat gggtgccgcc ggtacaccgt
     1441 tgaccacggc cgacaaggcg ttgctgaccg tggacgtgtg ggaacacgcg tactacatcg
     1501 actaccgcaa tatgcgtccc aagttcgtcg aaacattcct cgacaagctg gtgaactgga
     1561 gctttgcgga agccaatttc acctgaagaa gcagggcgcg acaaaagggc tcgaagggca
     1621 actttcgggc cctttttgct gcacggttgt tttggaaact gaaaaaatat gcaattaaat
     1681 cgcagttaat tttgcattgt gatattgaat tgcaaaaaat gcggcttggg tcagaccact
     1741 gaaagcacaa tcaccgacta tcttctggct aaagtcagtt ggtgcgaatc agactggcgg
     1801 tatttcctaa ctatttttgg agacaagaaa tgagcaataa gcaaccgacc atcatctaca
     1861 cgttgaccga cgaggcgcct cgtctggcca cggcttcctt tttacctatc gtgcgtgcat
     1921 ttgcctcggc tgctggcgtc gatgtgctgg aaagcgatat ctcggttgct gcgcgtgttc
     1981 tggcgacttt tcccgagtgc ctgaccgaag cgcagcgcgt gaccgacaac ctggcagagc
     2041 tgggcaaact cacgctcctg cccgatacca acatcatcaa gctgcccaat atcagtgcat
     2101 cggtaggaca actggttacc tgtatcaagg agttgcagtc caagggttat gcggtgcccg
     2161 actaccccga ggcacccaag accgaagaag aaaaagccat tcgcgcccgc tatgccaagt
     2221 gcattggcag cgcggtgaat ccggttctgc gcgaaggcaa ctcggaccgc cgtgcgcccc
     2281 gtgcggtcaa ggaatatgcc cgcaagaatc cgcacagcat ggccgagtgg agccaggcct
     2341 cgcgctcgca cgtctcccac atgcaccacg gcgacttcta ccacggtgaa aagtccatga
     2401 cactggacaa ggcgcgcgat gtcaagatgg aactgctcac caagagcggc aagaccattg
     2461 tgctcaagcc caaggtctcg ttgctggacc gcgaggtcat tgacagcatg ttcatgagca
     2521 agaaagcact gctggaattc tatgaaaagg aaatcgaaga cgcgcgcaag accggtgtga
     2581 tgttctcgct gcacgtcaag gccaccatga tgaaggtgtc gcaccccatc gtgttcggcc
     2641 actgcgtcaa gattttctac aaagacgcat tcgagaagca cgccaagctg ttcgaagaac
     2701 tgggtgtcaa cgtcaacaat ggcatggcca atctgtacga caagatcgcc accttgccac
     2761 aaagcacaca ggacgaaatc aagcgtgacc tgcacgcctg tcacgagcac cgccccgaac
     2821 tggccatggt ggattcggcc aagggcatta ccaacttcca ttcgcccaac gacgtgatcg
     2881 tggacgcctc catgcccgcc atgattcgca acggcggcaa gatgtgggac gccaatggcc
     2941 gcctgaagga tgtgaaggcg gtgatgccag agtccacttt cgcccgcatc taccaggaga
     3001 tgatcaactt ctgcaagtgg cacggtgctt tcgatcccaa gaccatgggc acggtaccca
     3061 acgtgggcct gatggcccag caggctgagg aatacggctc gcacgacaag acctttgaaa
     3121 tcaccgagga tggggtggcc aacattaccg atcttgcgac cggtgaggtc ttgatgagtc
     3181 agaacgtgga aaccggtgac atttggcgca tgtgccaagt caaggacgcg gcgatccgcg
     3241 actgggtcaa actggccgtc aaccgcgcac gcaattccgg tatgcccgtt gtgttctggc
     3301 tggattctta ccgtccgcac gaagcgcagc tcatcaccaa ggtcaagatg tacctgcatg
     3361 agcacaacac cgctggtctg gacatccaga tcatgagcca ggtgcgtgcc atgcgctaca
     3421 cactggagcg cgtgatccgt ggcgaggaca ccatcagcgc taccggtaac atcctgcgcg
     3481 actacctgac cgacctgttc ccgatcatgg aactgggcac ctcagccaag atgctgtcga
     3541 tcgtgccatt gatggccggt ggtggcatgt atgaaactgg cgccggtggt tcggctccca
     3601 agcacgtgca acagctggta gaagaaaatc acctgcgctg ggattcgctg ggcgagttcc
     3661 tggcgttggc ggtgtcactg gaggatctgg ggctgaagac cggcaatgcg caggccaaga
     3721 ttctcgcgaa gaccttggat gcggccactg gcaagctgct ggacaacaat aaaaatccgt
     3781 cgcccaagac cggtcagctc gataaccgtg gcagccagta ctacctggcc atgtactggg
     3841 cccaggagct ggcagcgcag agcgatgacc gtgaacttgc cgctcgattc acgtccctgg
     3901 ccaagcaatt gacggacagt gagcaggctg tcgttgctga attggcggcg gtgcaaggaa
     3961 agccagcgga tattggtggc tattacctgc ctgacctcgg caagctggat gcagtgatgc
     4021 gtcccagcaa aacgctgaat acagttttgg catccttgaa gtcgtaatcc gccgcgcagg
     4081 ctatgtgcct gtcttgacgt caaaggcgat tcagactccg gtctggatcg cctttttaat
     4141 tgcttctgcc gtcgaacaat gggcctgtga gttttgctcc ggctttgatg ctgcgtttgg
     4201 caaaccaccc ctggttcatt tccagcacga agcggactgg tttggctgcg caatgcgagt
     4261 cggttgtctg tggcttcata tctgccagat ttacgatggt gccgtcatca gccacaaagg
     4321 ccgcagtgag tggcaacaat gtgtttttca tccagaaaca cagtttggat ggctggctga
     4381 atacgaagac cattccttcg tgcggtggca tttcacggcg aaacatgagt ccgatcgctt
     4441 gctgctcctg ggtttgcgcg acttgtgcat cgatctggtg aattcccgca cccagcttga
     4501 cacggggcaa attcagctgg ggtgaatcct gtgcgagggc agatcctgcc acgaccatga
     4561 gcagcggaag caatcgggtg aggaatgatt tcatgcgttg aattgtcagt gattcgcgtg
     4621 attgaggtgg gcaaaatata caactagaat gggtcgcatc gttgcccacg ccagcggttt
     4681 ctggctgtag ggtagcttgt ggcaagagcg ttttcgcgtt gaatacggag tcctgttttt
     4741 atgtaccagc atatcaaggt gcctgcccaa ggccaaaaaa tcaccgtcaa tgcagacatg
     4801 acattgaatg tgccggatca accgatcatt ccctacatag aaggggacgg tacgggtctg
     4861 gatatcactc cggtgatgtt gaaagtggtg gatgctgcgg tggccaaggc ttacggcggc
     4921 gcgcgcaaga ttcattggat ggaagtctat gccggcgaga agtccaccag aatttatggc
     4981 ccggatgtct ggctgccgca agagacgctg gatgtcgtgc gtgagtatgt ggtgtctatc
     5041 aaggggcctc tgacaacgcc tgtgggtggt ggcatccgtt cactgaacgt tgcgctgcga
     5101 caggagctcg acctttacgt ttgcctgcgt cccatccagt acttcaaggg tgtgcccagt
     5161 ccgctgaaag agccagagaa gaccaatatg gtgatcttcc gtgaaaactc ggaggatatt
     5221 tatgcgggca tcgagttcga agccgagtcg gacaaagcca agaaactgat caaatttctg
     5281 caggatgagt tcggcgtcac aaagatccga ttcccgaata cctccggtat aggtatcaaa
     5341 cccgtgtcgc gcgaagggac cgagcgtctg gtgcgcaagg ccattcagta cgccatcgac
     5401 aatgacaagc ccaatgtgac cattgtgcac aagggcaaca tcatgaaatt cacagagggt
     5461 ggcttccgtg attggtccta cgctctggcg cagcgcgagt tcggtgccga actgatcgac
     5521 ggtggaccct ggtgcaagtt caagaatccc aggacgggca agtacatcac catcaaagac
     5581 agcattgcgg atgctttcct gcagcagatt ctgttgcgtc cttccgagta cagcgtagtc
     5641 gctacgctga acctcaatgg tgactacatc tccgatgcgc tggcagcgca ggtcggaggg
     5701 atcggcattg cgcctggcgc caatctttcc gataccgtcg ccatgttcga ggcaacgcac
     5761 ggaacagcgc ccaagtacgc gggaaaagac tatgtgaatc ccggttcaga aatactgtct
     5821 gctgaaatga tgctgcgtca catgggttgg aaggaagcgg ccgatctgat cattcgatcg
     5881 attgagcgat ccattgccag caaaaaagtg acttacgact tcgctcgcct gatggatggt
     5941 gccagccagg tcagttgttc gggctttggt caggtgatga tcgataacat gtaaccgttt
     6001 cgtaatgccc ttgttgggcg tgcacggccg gagtaactgg aaccgcaggg gcagggcctt
     6061 tgcggttttt tcttttttta ggccttgatt gcccgcactt tgtcgccaca tcactcttgc
     6121 ggtgcagcaa tccggcgcga ccgatagaat cttttgcatg gcttcaaata aaccgaccca
     6181 accgcctgta cccccggtgc aagtgcccga aagagatgac agcaatgctg tggtgctgga
     6241 gcgccgcaca cagcgtgttg ggcccccgca tatgtaccag gttgtcatgc tcaatgatga
     6301 ttacacgccg attgagtttg tggtggtggt c
//
LOCUS       DAOSJM010000115         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11133_length_6331_cov_3.940567, whole genome shotgun sequence.
ACCESSION   DAOSJM010000115 DAOSJM010000000
VERSION     DAOSJM010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11133_length_6331_cov_3.940567"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PKX82_05515"
     CDS             <1..468
                     /locus_tag="PKX82_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HOF51744.1"
                     /translation="ALDETIRTRFGDTLEAAARCELFAWRATAEGRLAEIIVLDQFSR
                     NVYRDTPRAFAQDALALVLAQELVASTQDRSLPAAQRVFAYMPYMHSESALVHTQAVA
                     LFTQLGNQDNLNFELRHKAIIDRFGRYPHRNAILGRSSSAEELAFLSEPGSSF"
     gene            621..1103
                     /locus_tag="PKX82_05520"
     CDS             621..1103
                     /locus_tag="PKX82_05520"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF51745.1"
                     /translation="MTLHDLAPIIHARRTALGLSQDRLAKLCGLSRATVNQLENGTLV
                     DLGAAKLLALLALLGLELNAHRKPAPLDALALLSQTASVSYRRVLQPAELARALAQGD
                     LPEAITPHIATLLDEAPLTLILAAVEQVAHTTRTPPKTLWKHLVQWAHALQSPTTAWA
                     "
     gene            1061..1636
                     /locus_tag="PKX82_05525"
     CDS             1061..1636
                     /locus_tag="PKX82_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOF51746.1"
                     /translation="MGACLAVTHHGMGLMHMAAHPHQPEGLPDGVWRGLLQQAFSLVD
                     EIALHGLPQPFWTFGGGTVLMLRYRHRFSKDVDIFVPDPQYLGFVSPRLSDVAESVTH
                     DYVEGPGYVKLLRPEGEIDFVASPNLTDTPFELWHPGVKCCSPSSKPSTHWTTGPALT
                     TPLRCLRSFSIRYNFSSCLRILHEGWRPIYL"
     gene            1649..2638
                     /locus_tag="PKX82_05530"
     CDS             1649..2638
                     /locus_tag="PKX82_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51747.1"
                     /translation="MHSLGSTLPVTFLRTLTLAPAQHPKGLAHMSAASGLVRVLRRLY
                     VVADDELHLGLFDDIGPSGADAAPGALLRLFEGDLPLQKEQRKATKPDLETLALLPPL
                     PGCPAGALLALGSGSKPQRETGVLLALDAQGAPTGHQTRIDLAPLYAPLRTHFADLNV
                     EGALLVGDELLLLQRGNQADARSACVRYAWPHVADWLAGAMTGTAAEPPAPRHVQPMV
                     LGTVDGVPLALTDGTPLQGGAWAFCAVAEATDNSYQDGRCVASAIGIVAPDGQMRQLH
                     LLQGAPKVEGIAAQWVGNSWQFTMVTDPDDRAIAAQLLQATLGHASTDLLLFQ"
     gene            2713..3003
                     /locus_tag="PKX82_05535"
     CDS             2713..3003
                     /locus_tag="PKX82_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOF51748.1"
                     /translation="MRPSEALSLHRTQIREIALRHRVSGVRVFGSALHGDDTADSDLD
                     LLVEPTAQTTLMDIGAIRFELKQLLGMDVDVLTPNSLPASFRDQVLREALAV"
     gene            3000..3353
                     /locus_tag="PKX82_05540"
     CDS             3000..3353
                     /locus_tag="PKX82_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020207092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOF51749.1"
                     /translation="MSRAHPLRVADYLQHILEAIGNIQEYTTGMNLAGFMADRKTCDA
                     VIRNLEVLGEACNNIAKNHPDFAAQHATVPWGFAYEMRNALAHGYFTVDLAIVWQTIQ
                     NDLPSLHKQVTELPR"
     gene            complement(3532..4050)
                     /locus_tag="PKX82_05545"
     CDS             complement(3532..4050)
                     /locus_tag="PKX82_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51750.1"
                     /translation="MLYLKCTGIVQKALGLPKTVVADAIPSSAPLGNWYVNRFDTGRR
                     KAYVFMSETTLLSFILFQGKKPVTTETLPTMMLGGLAQLLQMRGMVEEAIERAMLPYH
                     EGVFAKTDSRPDLGSLNDLVQRYQWLIEYEGGLDRCDLTGIIMGINNTPQRRLGWCSS
                     WDMTQSKLQLPN"
     gene            4161..4646
                     /locus_tag="PKX82_05550"
     CDS             4161..4646
                     /locus_tag="PKX82_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF51751.1"
                     /translation="MTNQPIQSDKSGIAQLQALGAQIRARRKALRLNATLTAEAAGMS
                     RVTLHRIEKGEPSVTMGAWSNALAALGMSLQAVITADESKRTAAQPDRTGWIPARVSL
                     SEYPQLRALAWQVHGTDTLTPTEALDIYERNARHLDVPAMSADEQALLQALRIAFSHA
                     V"
     gene            4636..5349
                     /locus_tag="PKX82_05555"
     CDS             4636..5349
                     /locus_tag="PKX82_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOF51752.1"
                     /translation="MQFDRPHHQRIAHVLNALNGATLLAHGCYFGGGTCIALHHGEYR
                     ESVDIDFLVSDAAGYRELRQQLTHPAGLAAITHPHTPPLVALREIRADQYGIRTQIQM
                     DGHSIKFEIVREARIALEKPGASDAICGVPTLTRLDLATSKLLANSDRQADDGVFSRD
                     VIDLAMMGLPLPALRAALTKAEQAYGPSVARDLGKAIDRLQHRQGWLERCMQVMAMQM
                     PKAVLWQKLRSLRRIVPAG"
     gene            complement(5382..6137)
                     /locus_tag="PKX82_05560"
     CDS             complement(5382..6137)
                     /locus_tag="PKX82_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOF51753.1"
                     /translation="MATNTNQKLAVLIDADNAQASVIQELLAEVSRYGTATIKRAYGD
                     WTTQNLRSWKDVLHTMAIQPIQQFSYTSGKNATDSALIIDAMDVLHTGSVDGFCLVSS
                     DSDFTRLATRIREAGLVVYGFGERKTPEPFVAACDKFIYTEILRNEPDEASPNQQQQP
                     VAELPKLKPILLNALNATAREDGWTTLSALGSQINRSHPSFDPRNYGVAKLGELIRQQ
                     AAYLEVKEVKTGEGDSVQTHLHVRRKAAGGAKT"
BASE COUNT         1174 a         1971 c         1911 g         1275 t
ORIGIN      
        1 gcgctggatg aaaccatccg cacccgcttt ggcgacacgc tagaagccgc ggcgcggtgt
       61 gagctgtttg catggcgcgc cacggctgag gggcggctgg cggagatcat cgtgttggac
      121 cagttctcgc gtaacgtgta ccgcgacacg ccgcgcgcct ttgcgcagga cgcgctggcg
      181 ctggtgttgg cgcaagagct ggtggcaagt acgcaggacc gcagcctgcc cgcggcgcag
      241 cgcgtgtttg cctacatgcc ctacatgcac agcgagtccg cgctggtgca tacgcaggcg
      301 gtagcactgt ttacgcagct gggcaaccag gacaacctga acttcgagct gcgccacaaa
      361 gccatcatcg accgctttgg ccgctacccc caccgcaacg cgattctggg gcgcagttcc
      421 agcgcggagg agctggcgtt tctgagcgag ccggggtctt cgttctaggg ctgcgtcttg
      481 tgtttgctac ttttttagta gctgcttgtg catattccac gggggctgga ggccaatctg
      541 gcacagaaat ccagcgaatg tgtcaaattt ttatgtaaag catgtttgac attttttaag
      601 cgcagtgctc tacttcgccc atgactttgc acgacttggc ccccatcatt catgcccggc
      661 gcaccgcttt gggtctgtcg caagacaggc tggccaagct gtgcggtctg tcgcgggcca
      721 cggtgaacca gttggaaaac ggcacgctgg tggacctggg cgcggccaag ctgctggcgc
      781 tgctcgcttt gctggggctg gagctgaatg cccaccgcaa gcccgcccca ctggacgcgc
      841 tggcgctgct aagccaaacc gccagcgtga gctatcggcg cgtattgcaa cccgccgagc
      901 tggcccgggc gttggcgcag ggcgacttgc ccgaagccat cacgccccac attgccaccc
      961 tgctggacga agccccgctg acgctcatcc tggccgccgt ggagcaggta gcacacacaa
     1021 cccgcacccc gcccaaaacg ctgtggaagc atttggtgca gtgggcgcat gccttgcagt
     1081 cacccaccac ggcatgggcc tgatgcacat ggcagcgcac ccgcaccaac ccgagggctt
     1141 gcctgatggc gtatggcgag gccttttgca gcaagcgttt agcctggtgg acgagattgc
     1201 cttgcacggc ctgccgcagc cgttctggac gttcggcggt ggcacggtgc tcatgctgcg
     1261 ttaccgccac cgcttcagca aagacgtgga catttttgta ccagacccgc aatacttggg
     1321 ctttgtatcg ccgcgcctga gcgacgtggc cgagtccgtc acacacgact atgtggaagg
     1381 cccaggctac gtgaagctgc tgcgccccga gggtgaaatt gatttcgtgg cttcgcccaa
     1441 cttgacagac accccgtttg agctgtggca tcccggcgta aagtgctgca gtcccagttc
     1501 gaagccatcg acacattgga ctaccggccc agctttgacg acgcctttgc gctgtctgcg
     1561 gagtttctca attcgctata actttagtag ctgcttgcgc atactccacg agggctggag
     1621 gccaatttac ctctgaaaat cacaccccat gcacagcctt ggctccaccc tgcccgttac
     1681 gttcctgcgc acgctcacgc tggcaccggc gcagcatccg aaggggctgg cgcatatgag
     1741 tgcggccagt gggctggtgc gggtgctgcg gcggctgtac gtggtggcgg acgatgagct
     1801 gcacttgggc ctgttcgacg atatcgggcc atcaggggcc gacgcggccc ccggcgcgct
     1861 cttacgcctg ttcgaaggcg atctgccact gcagaaagag cagcgcaagg ccaccaaacc
     1921 cgatctcgaa accctggccc tgctcccgcc cctgccgggc tgcccggcgg gtgcgctgct
     1981 ggcgctgggc tcgggctcga agccgcagcg cgaaaccggc gtgttgttgg cgctggatgc
     2041 acagggcgca cccaccggcc accagacccg cattgatctg gccccgttgt acgctccact
     2101 acgcacccac tttgccgacc tgaacgttga aggcgcgttg ctggtgggcg acgagctgct
     2161 cctgctgcag cgtggcaacc aggctgacgc ccgcagtgcc tgcgtccgct acgcttggcc
     2221 gcacgtggca gactggctgg ccggtgcaat gacaggtaca gcggccgagc cccctgcacc
     2281 gcgacatgtt cagcctatgg tgcttggcac ggtcgacggc gtaccgttgg cgctaaccga
     2341 cgggacgcca ctgcaaggcg gtgcctgggc gttctgcgcc gtggcagaag ccaccgacaa
     2401 cagctaccag gatggccgct gtgtggcatc ggccatcggt atcgtggccc ccgacggcca
     2461 gatgcgtcaa ttgcacctgc tgcaaggcgc gcccaaggtg gagggcatag cagcacagtg
     2521 ggtgggcaac agctggcagt tcaccatggt gaccgacccg gacgaccgag ccatagcggc
     2581 acagctgctg caagccacgc tgggccacgc ttctaccgat ttgctacttt ttcagtagct
     2641 gcttgcgcat attccacggg ggctggaggc ccatttggca cataaatggc tcactccgtt
     2701 aaacttcagt ctatgcgccc ctccgaagcc ctctccctgc accgcacgca aatccgcgag
     2761 attgcgctgc gccaccgcgt cagtggtgtg cgggtgttcg gctcggcact gcatggcgat
     2821 gacacggcgg acagcgacct ggacctgctg gtggaaccca ccgcgcaaac cacgctgatg
     2881 gacattggtg ccatccggtt tgagctgaaa cagcttttgg gaatggatgt ggatgtgctc
     2941 actcccaaca gcctgccagc cagctttcgc gatcaggtgc tgcgcgaggc cttggccgta
     3001 tgagccgtgc gcacccgctg cgggtggcag actacctgca gcacatcctg gaagccattg
     3061 gcaacattca ggaatacacc acgggcatga acttggccgg attcatggca gaccgcaaga
     3121 cctgcgatgc cgtcatccgc aatctggaag tccttggcga agcctgcaac aacattgcca
     3181 aaaaccaccc cgactttgct gcgcagcacg ctaccgtgcc ttggggtttt gcctacgaaa
     3241 tgcgcaatgc gctggcgcac ggctatttca cggtagatct tgccatcgtg tggcaaacca
     3301 ttcagaacga tctgccgtcg cttcacaagc aggttactga gcttccacgc tagcttgcta
     3361 cttttttgat agctgcttgc gtatattcca cggaggctgg agggcaattc cattgccaat
     3421 ttccaatgaa acccaattcg ggtttcttcc accccaatat gggtatttac atcaagtcaa
     3481 ccgaaccctc gccccatggg ccaccacgat cggtacgcac gcgtgatgcg gtcagttggg
     3541 tagttgtagt ttgctttggg tcatgtccca ggaactgcac caaccgagcc tgcgctgtgg
     3601 cgtgttgttg atgcccatga tgatgcctgt gagatcgcac cgatcgagcc ccccttcgta
     3661 ctcaatcagc cattggtacc gctgcaccag gtcgttgagc gaacccagat ccgggcggct
     3721 atcggttttt gcaaacaccc cttcgtggta cggcagcatc gcccgctcga tggcctcctc
     3781 gaccattcca cgcatttgga gaagctgcgc cagcccgcca agcatcatgg tggggagcgt
     3841 ttctgtcgtc acgggcttct tgccctggaa caggatgaat gaaagcaggg tcgtctcgct
     3901 catgaaaacg taggccttgc gccgcccggt atcgaaccgg ttgacgtacc agttgcccaa
     3961 gggggctgag ctggggatgg cgtcagccac aacggtcttg ggcaggccca gggctttttg
     4021 aacgatgcct gtgcatttca aatagagcat cgcgttattg tcgagcagtt gggtgactac
     4081 aaagtcaaga ccgttttact gaataaaact tgtttcccat atttgacaaa catgctattt
     4141 tgcaaataat gagaaacatt atgacaaacc aacctatcca atcagacaag agcggcatag
     4201 cccaactgca agccttgggc gcgcaaatac gtgcccggcg caaggctctg cgcctgaacg
     4261 ccacccttac tgccgaggca gcaggaatgt cacgcgtcac gctgcaccgc atagaaaagg
     4321 gtgagccgtc tgtaaccatg ggcgcgtggt ccaacgcgct ggcagcgttg gggatgtcac
     4381 tgcaggccgt catcacggcc gacgagagca agcgcaccgc agcgcagcca gatcgcacag
     4441 ggtggattcc cgcgcgcgtg tcgctgtcgg aatacccgca gctgcgtgcg ttggcttggc
     4501 aggtacacgg caccgacaca ctgacgccca cagaggcact ggacatatac gaacgcaacg
     4561 cccgccatct ggatgtgccc gccatgtccg cagacgagca ggccctgctg caggccctgc
     4621 gcatagcttt cagccatgca gtttgaccgc ccccaccacc agcgcattgc ccatgtgctg
     4681 aacgccctga acggtgccac gctgcttgcg cacggttgct attttggcgg cggcacctgc
     4741 attgccttgc accacggcga ataccgtgaa tcggtagaca tcgatttttt ggtgtccgac
     4801 gccgcaggct accgcgagct gcggcagcag ctgacacacc ctgccggctt ggcagccatc
     4861 acacatccgc acacaccgcc cttggtggct ctgcgcgaga tacgggcgga ccaatatggc
     4921 attcgcaccc agattcagat ggacggacac agcatcaagt ttgagatcgt gcgcgaagca
     4981 cgcattgcgc tggagaaacc cggtgccagc gacgccattt gcggtgtgcc cacgctaacc
     5041 aggctggact tggccacgtc caaactcctt gccaactcag accggcaggc cgatgacggt
     5101 gtgttcagcc gcgacgtgat tgaccttgcc atgatggggc tgcccctacc cgcactgcgt
     5161 gccgcactca ccaaggcaga acaggcctat ggccccagcg tcgcgcgcga cctgggcaag
     5221 gccattgacc gcctgcaaca ccggcagggt tggctggagc gctgcatgca agtcatggcc
     5281 atgcaaatgc ccaaagcggt gttgtggcaa aagctgcggt ctttgcgccg gatagtgcct
     5341 gccggctaat tggcgtttct cgccgagccg aggttctcgt tctaggtctt ggcaccacca
     5401 gccgctttgc gccgcacgtg cagatgtgtc tgcaccgaat cgccctcgcc ggtcttcact
     5461 tctttcactt ccagatacgc tgcctgctgg cggatcagtt cgcccagctt ggccacgccg
     5521 tagttgcgcg ggtcaaatga ggggtggctg cggttgatct ggctgcccag ggctgatagc
     5581 gtggtccagc cgtcttcgcg ggcggtggcg ttcagcgcgt tgagcaatat cggcttgagc
     5641 ttgggcagct cggccacggg ttgctgttgc tgattcgggc tggcctcgtc cggctcgttg
     5701 cgcaagattt cggtgtagat gaacttgtcg cacgcggcca caaagggctc gggcgtcttg
     5761 cgttcgccaa agccatagac caccagcccc gcctcgcgga tgcgggtggc caggcgggtg
     5821 aagtcgctat cgctgctgac caggcaaaag ccgtccacgc tgccggtgtg cagcacgtcc
     5881 atggcgtcga tgatcagggc ggagtcggtg gcgttcttgc cgctggtgta gctgaactgc
     5941 tgtatgggct ggatggccat ggtgtgcagc acgtctttcc agctgcgcag gttctgcgtg
     6001 gtccagtcgc cataggcgcg cttgatggtg gccgtgccgt agcgcgaaac ctcggccagc
     6061 agctcctgta tgacggaggc ctgcgcgttg tcggcgtcaa tgaggacggc gagcttctgg
     6121 ttggtgttgg ttgccatgga gacccccctt gttttcacgt catcgtacag cggatagatt
     6181 tgctaatttt tagtagctgc ttgcgcatat tccacggggg atggaggcca cgacttccac
     6241 tgcacccgat tcaacgtctc ctgccccgcc tttgtcgggc gaactacact gcaccaagcg
     6301 cccgctcagt taaatggcgg acgacggcat t
//
LOCUS       DAOSJM010000116         6320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11158_length_6320_cov_4.444533, whole genome shotgun sequence.
ACCESSION   DAOSJM010000116 DAOSJM010000000
VERSION     DAOSJM010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11158_length_6320_cov_4.444533"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..1094)
                     /locus_tag="PKX82_05565"
     CDS             complement(57..1094)
                     /locus_tag="PKX82_05565"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOF51754.1"
                     /translation="MNHRPIPAERLPELLRRMPKAELHMHIEGSLEPDMIFSMGQRNQ
                     VPLNYPSVDALRQAYVFNNLQEFLDIYHAGTTVLKTEQDFYDMTCAYLARAQADNVLH
                     TEIFFDTQTHTGHGLSAEVVINGLHRACMDAPAKFGMTASLILCFLRHLSEEEAFECL
                     EQALLLRDKIVGIGLASSEVGHPPEKFAKVFARARELGFRLVAHAGEEAPPAYIWSAL
                     DVLKVERIDHGVQAIHDAALMRRLAEEKIPLTVCPLSNLKLQVFKNLSQHNLGRMLDA
                     GIVATINSDDPAYFGGYINENFTQTFAALGLTSQHAYTLARNSFEASFLEASAKARLI
                     ERLDGVFASLE"
     gene            1233..1508
                     /locus_tag="PKX82_05570"
     CDS             1233..1508
                     /locus_tag="PKX82_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOF51755.1"
                     /translation="MSDHYTTLGLTSAAKLADIKKAFRQKAALHHPDRNEAPEAAERF
                     RAVQKAYEVLSDETQRQAYDDNRRRNLLDDPLQTAREIWGTYFKSIL"
     gene            1521..2102
                     /locus_tag="PKX82_05575"
     CDS             1521..2102
                     /locus_tag="PKX82_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51756.1"
                     /translation="MNITPFFRDLRNSYQAEIDDMTFDSEGQDVLRQRLAQRRKEMDF
                     LVHMMEPSPEMVAVVFHQGFRFKHPAVMEHLLTQHSDELPTWDALSSSIELMPWAQEL
                     AGVALKQPTGDWFLTVSAGLEYMYHRHDSTLAAASHSDDEDNEDEENAQGHADNAGDD
                     RPDPVDSDDAHDAKAREEAGADWMVEQGFDRKD"
     gene            2120..2875
                     /locus_tag="PKX82_05580"
     CDS             2120..2875
                     /locus_tag="PKX82_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51757.1"
                     /translation="MNHLALIEKTQSLIAAGDIVGAESALVELADTEGDGALMVVLEQ
                     LPPKDILAVIREYDTSKESIVNLLVTPEQFARAVVIEKQYKDLTRTHLRGMVNSIVFR
                     EDADPMEFLNAIGDLEGGSEALADYFSEKWARIESFARTGTFDASDDDGQVLSEAALY
                     SSAYASRRLEHDEVADQDWMELAYLLRYENPDLFIETVLVLRAKARAHDLGEEEEDAT
                     AAAEEDDGKVETANTDRGQATPAALEADEESAI"
     gene            2881..4089
                     /locus_tag="PKX82_05585"
     CDS             2881..4089
                     /locus_tag="PKX82_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51758.1"
                     /translation="MQAISLFDGRPFFEKALAYGVQHGLLTPQKLDAMCQEAPKGMVQ
                     IARYFGTEFLRPELERAKDRMVNLISLHLEATTHGDLQQAAELLRDNSLLSRSKAGSD
                     MLKALIAMPQNSHFGMHEPGGFQDTHIPVLAKWALQSLPQYQAELAKRNAVQQLVDAA
                     LWMAEQLGMDEEALQESNADAEAVMRTALIALAAKRTEMPDWVDFAKLIASASKKIAT
                     APASAVIPLPKGVPPELLPTVETMRQSVWDDLPKIADSTVPARKLFDQTPAFMGRYFW
                     LEDGLAEVDHYHRQVSATWEKATGGRHDDSTLLTLFLTLAAGATQKTLLTEKTAATLV
                     RKIRKSGLHPELTTAFITQHAPDAQRNDYLQLWQHFVDEAQATLESDRDYALNDALSL
                     LRRECNVKAS"
     gene            4121..4975
                     /locus_tag="PKX82_05590"
     CDS             4121..4975
                     /locus_tag="PKX82_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOF51759.1"
                     /translation="MKLLRYGPAGQEKPGVLDAQGQVRDLSGVIPDLAGNALLPASLA
                     RLQALAIDTLPIVPGTPQKDLRLGPCVGNIGKFICIGLNYSDHAAEAGMQVPPEPVVF
                     NKWTSAVVGPDDAVEIPRGSVKTDWEVELGVVIGQGGRYIAEADAMAHVAGYCVVNDV
                     SEREYQLERAGTWDKGKGCDTFGPTGPWLVTADEIPDPQALHMWLEVDGKRYQNGSTA
                     TMVYQVPFLISYLSRFMSLQPGDVISTGTPPGVGMGHKPPVFLRAGQTMHLGIDGLGT
                     QTQRIVQA"
     gene            4980..5438
                     /locus_tag="PKX82_05595"
     CDS             4980..5438
                     /locus_tag="PKX82_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOF51760.1"
                     /translation="MPRIQLTPAEHYPFVTEIQIYISHVNQGGHLDNAQLLALVSEAR
                     VRFFKSLGYFEAGVEGTLIVVGDIVAQYKSEGYHGETMRVEMAAADFNKYGFDLQFRM
                     SEKATSRDVALGKIGIVFIRPENRKVTPIPTAFLTRVEQLSGTSSAPANC"
     gene            complement(5435..6169)
                     /gene="ytfE"
                     /locus_tag="PKX82_05600"
     CDS             complement(5435..6169)
                     /gene="ytfE"
                     /locus_tag="PKX82_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HOF51761.1"
                     /translation="MNARLQTTEPADFVLNADQAIGQIAVNLPGSTAVFRRLKLDFCC
                     GGQISLKQAAADKGLDVQSVLQELAGLQRTQETAEMTDPAALVYHILSRYHEVHRQQL
                     PELIRMARRVEAVHRDHPLVPQGLAELLEAMEEELLSHMEKEETILFPMLTAGGSPFV
                     TAPIDMMRSEHTDHGAMLGAVLELTQDATPPDGACNTWRALYAGIAQLNDDLINHIHL
                     ENNVLFPQFEAAVAEQGCGSGCGCAG"
BASE COUNT         1368 a         1942 c         1874 g         1136 t
ORIGIN      
        1 gaggcgtgtt gaaaaaatgg tagcacgtcc gatgtctcaa gtctccagaa aaaacactac
       61 tccaagctgg caaacacgcc atccagccgc tcgatcagac gggctttggc cgatgcctcc
      121 agaaaactgg cctcaaaact gttgcgggcc agcgtgtagg cgtgctgcga tgtcagtccc
      181 agcgcagcaa aggtttgcgt gaagttctcg ttgatatagc caccaaagta ggccgggtca
      241 tccgagttga tggtggccac gatgccggcg tcgagcatgc ggcccaggtt gtgctgggac
      301 agattcttga acacctgcag cttgaggttg gacagggggc agaccgtcaa cggaatcttc
      361 tcttccgcca gccgccgcat aagggcagcg tcgtggatgg cctgcacacc atggtcgatg
      421 cgttccacct tgagcacatc cagtgcgctc cagatatagg ccggcggcgc ctcctctccc
      481 gcatgggcca ccaggcgaaa gcccagttct cgggcgcggg cgaacacttt cgcaaacttc
      541 tcgggcgggt ggccgacttc gctggacgcc aggccgatgc ccacaatctt gtcgcgcagc
      601 agcagtgcct gctccagaca ttcaaaagcc tcttcctcac tcaaatgccg caggaagcac
      661 agaatcagcg acgccgtcat gccaaacttg gccggggcgt ccatgcaggc acggtgcagg
      721 ccgttgatca ccacctccgc actgaggcca tggccggtat gcgtctgggt gtcaaagaag
      781 atttcggtgt gcagcacgtt gtcagcctgc gcgcgcgcca ggtaggcgca ggtcatgtca
      841 tagaagtcct gctcggtctt gagcacggtg gtgcctgcgt ggtaaatatc cagaaactcc
      901 tgcaggttgt tgaacacata agcctggcgc agggcgtcca cgctggggta gttcagcggc
      961 acctggttgc gttggcccat ggaaaaaatc atgtcgggtt ccagcgagcc ctcgatatgc
     1021 atgtggagct cggccttggg catgcggcgc agcagctcgg gcaggcgctc ggcagggatg
     1081 ggacggtggt tcataggggg cctccaaagt cagggttcag tgcactctac agcacccaag
     1141 tggcctggcc cacacaggct tttgaccaag tctgcgaaaa tagccggtta agtcaatttt
     1201 ctccggtgct gatcggaatc taaccacgcg ttatgtctga ccactacacc accctgggcc
     1261 tcacgagcgc tgccaagctt gccgatatca aaaaggcctt tcggcagaag gctgccctgc
     1321 accacccgga ccgcaacgaa gcgccggagg cggcagagcg gtttcgggcc gtgcaaaaag
     1381 cctacgaagt gctgtcagac gaaacgcaac gccaggccta cgacgacaac cgccggcgca
     1441 acctgctgga cgacccgctg caaaccgccc gcgaaatctg gggcacctat ttcaaatcca
     1501 ttctttaacg tttgcactgc atgaacatca ccccgttttt ccgcgacctc cgtaattcct
     1561 accaggctga aattgatgac atgacgtttg actccgaggg tcaggatgtg ctgcgccagc
     1621 gcctggcaca gcgacgcaag gaaatggatt ttttggtcca catgatggag cccagcccgg
     1681 agatggtggc ggtggttttt caccagggct tccggttcaa gcaccctgcc gtcatggagc
     1741 acctgctgac ccagcacagt gacgaactgc cgacctggga cgcactgagc agcagcattg
     1801 agctcatgcc ctgggcgcag gagctggcgg gcgtggcgct gaagcagccc acgggcgact
     1861 ggtttcttac cgtctcggcg ggtctggaat acatgtacca ccggcatgat tccacgcttg
     1921 cagcggcatc gcacagcgat gacgaagaca acgaagatga ggaaaacgca cagggtcacg
     1981 cagacaacgc aggcgacgac cgtccggacc ctgtggattc cgacgacgcc cacgacgcca
     2041 aagcgcgcga agaggccggt gccgactgga tggtcgagca aggctttgac cgcaaagatt
     2101 gatggaagag caagccccca tgaaccacct cgccctgatt gaaaaaaccc aaagcctgat
     2161 tgccgctggc gacatcgtcg gcgccgagtc ggcactggtt gaactggcag acaccgaggg
     2221 cgacggcgcc ctgatggtgg tgctggagca attgccgccc aaggacatcc tggcggtcat
     2281 tcgcgagtac gacacctcca aagagtccat cgtcaacctg ctggtcacgc ccgagcagtt
     2341 tgcccgcgct gtggtgatcg aaaagcagta caaggacctc acccgcaccc atttgcgcgg
     2401 catggtcaat tccatcgtct tccgcgaaga tgccgacccg atggagttcc tgaacgccat
     2461 tggcgatctc gaaggcggca gcgaagcgct ggcggactac ttctccgaaa aatgggcacg
     2521 cattgaatcc tttgcgcgca ccggtacctt cgacgcgtcg gacgacgacg ggcaggtact
     2581 ctcggaagct gctctgtatt cctcggccta tgccagccgc cgcctggaac acgatgaagt
     2641 tgcggaccag gactggatgg aactggccta cctgctgcgc tatgaaaacc cggacctctt
     2701 tattgagacc gtgctggtgc tgcgcgccaa ggcacgcgcg cacgacctgg gcgaagagga
     2761 agaggacgcc acagcagcag ccgaagaaga cgacggcaag gtggaaaccg ccaacaccga
     2821 ccgcggccag gccacgcccg ccgcgctgga ggcggacgaa gaatccgcca tctgaaaccc
     2881 atgcaagcga tatccctgtt tgacggacgg ccgttctttg aaaaagcgct ggcctatggc
     2941 gtgcagcacg gcctgttgac accgcaaaag ctcgacgcca tgtgccagga ggcacccaag
     3001 ggcatggtgc agattgcgcg gtatttcggc actgagtttt tgcgcccgga actggaacgc
     3061 gccaaagacc gcatggtcaa tttgatcagc ctgcatctgg aagccaccac ccatggcgac
     3121 ctgcaacagg cagcggagtt gctgcgggat aacagcctgc tgtcgcgctc caaagcgggc
     3181 tccgacatgc tcaaggcctt gatcgccatg ccgcagaaca gccactttgg catgcacgag
     3241 cccggcggtt ttcaggacac gcacattcct gtgctggcca aatgggcact gcaatcattg
     3301 ccccagtacc aggccgaact ggccaagcgc aacgccgtgc aacagctggt ggacgccgcg
     3361 ctctggatgg cggagcaact gggtatggat gaagaggcgc tgcaggagtc caatgccgac
     3421 gccgaggcgg tcatgcgcac ggcactgatt gcgctggcag caaagagaac cgaaatgccg
     3481 gattgggtgg actttgccaa actgatcgcc agcgcatcca agaagattgc cactgcgcca
     3541 gccagcgcgg ttatcccgct gcccaaaggg gttccaccgg agctgctacc gacggtggaa
     3601 actatgcggc aatcggtatg ggatgacctg ccaaaaattg ccgacagcac cgtccccgcg
     3661 cgcaagcttt ttgaccagac gccggcattc atgggacgct atttctggct ggaagatggg
     3721 ctggccgaag tggaccacta ccaccgccag gtgtcagcca cctgggaaaa agccactggc
     3781 ggccgccacg acgacagcac gctgctcacc ctgttcctga cgctggccgc aggcgctacc
     3841 cagaaaactt tgctcacaga aaagacggct gccaccctgg ttcgaaaaat ccgcaagagt
     3901 ggattgcacc ccgagctgac aacggcgttc atcacccagc atgcgcccga cgcccaacgc
     3961 aacgactacc tgcaactgtg gcaacacttt gtcgatgagg cgcaagccac gctggagagc
     4021 gaccgcgact acgcgctcaa cgatgcgctg agcctgctcc ggcgcgaatg caatgtgaag
     4081 gccagctgag cgctagactg acctgaaaga aggagatgcc atgaaactac tgcgctacgg
     4141 ccctgcgggc caggaaaaac ccggcgtact cgatgcgcag ggccaggttc gcgatctgtc
     4201 gggcgtgatc cccgacctgg ctggcaatgc actgctgccc gccagtctgg cccggctgca
     4261 ggcccttgcc atcgacacac tgccgattgt tcccggcacg ccgcaaaagg acctgcgcct
     4321 ggggccctgc gtaggaaaca ttggcaagtt catctgtatc ggcctgaact attccgacca
     4381 tgcggcagag gccggcatgc aggtaccgcc agaacctgtg gtcttcaaca aatggaccag
     4441 cgccgtggtg ggcccggacg atgcggtgga gattcctcgc ggctcggtca aaaccgactg
     4501 ggaggtggaa ctcggcgtgg tcattggcca gggcgggcgc tacattgccg aagccgatgc
     4561 catggcccat gtggcaggct actgcgtggt caacgatgtg tccgagcgcg aataccagct
     4621 cgaacgcgca ggcacctggg acaagggcaa gggctgcgac acctttggcc cgaccggccc
     4681 ctggctggtg accgccgatg agataccgga cccgcaggcc ctgcacatgt ggctggaggt
     4741 ggacggcaag cgctaccaga acggctcgac cgccaccatg gtctaccagg tgccttttct
     4801 gatcagctac ctgagccgct tcatgagcct gcagccaggc gatgtcattt ccaccggcac
     4861 cccgcccggc gtgggcatgg ggcacaagcc cccggtgttc ctgcgcgcag gacaaaccat
     4921 gcacctgggc attgacggcc tgggaaccca gacacaacgc atcgtacagg cctgaaccca
     4981 tgccccgcat ccagcttacc cccgccgaac actacccctt cgttacggaa atccagatct
     5041 acatcagcca cgtcaaccag ggcgggcacc tcgacaatgc gcaactgctg gccctggtct
     5101 cggaggcgcg cgtgcgcttt ttcaagtcac tgggctactt tgaggcgggt gtggaaggca
     5161 cgctcatcgt cgtgggcgac atcgtggcgc agtacaagtc cgagggctac cacggcgaga
     5221 ccatgcgcgt ggaaatggcc gccgcagact tcaataagta cggctttgat cttcagttcc
     5281 gcatgagcga aaaagccacc agtcgcgacg tggcgctggg aaaaatcggg atcgttttca
     5341 tccggcccga aaaccgcaag gtcacgccga ttccgacagc ctttctgaca cgggtcgagc
     5401 agctgtcggg cacgtcatcc gcaccggcaa actgctaacc cgcgcagcca cagcccgaac
     5461 cacatccctg ctcagccacc gcggcttcga actgcggaaa caggacgttg ttttccaggt
     5521 ggatgtggtt gatcaaatca tcgttgagct gggcaatgcc cgcgtagagc gcccgccagg
     5581 tgttgcaggc accgtctggc ggagtcgcat cctgggtgag ttccagcacc gcaccgagca
     5641 tggcaccgtg gtcggtatgt tcgctgcgca tcatgtcgat gggcgccgtc acaaacgggc
     5701 tgccaccagc ggtcagcatg ggaaacagaa tggtttcttc cttttccatg tgcgatagca
     5761 attcttcttc catcgcttcc agtagctcgg ccaatccctg gggcaccagt ggatggtcgc
     5821 ggtggactgc ctccacacgg cgggccatgc ggatcagctc cggaagctgc tggcggtgga
     5881 cttcgtggta gcgggagagg atgtgataga ccagcgcggc cggatcggtc atttccgccg
     5941 tctcttgcgt tcgctgcagg ccagccagct cttgcagcac cgactgcaca tccagcccct
     6001 tgtccgcagc ggcctgtttc aggctgatct gtcctccaca gcaaaagtcc agcttcagac
     6061 gccggaatac ggccgtagaa ccgggaaggt tcaccgcaat ctggccaatc gcctgatcgg
     6121 cattcagaac aaaatctgcg ggctcagtgg tttgcaaacg ggcattcatg gcaacatcct
     6181 tttaaagatt catattaaat gaatgtattg tatcgaataa aattcataca aaatgaatct
     6241 ttaattttcg gaagttttca cccccccatg cgcctgaccc aatggactga ctacaccctg
     6301 cgtgtgctga tgtactgtgc
//
LOCUS       DAOSJM010000117         6275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11268_length_6275_cov_4.795016, whole genome shotgun sequence.
ACCESSION   DAOSJM010000117 DAOSJM010000000
VERSION     DAOSJM010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11268_length_6275_cov_4.795016"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKX82_05605"
     CDS             <1..309
                     /locus_tag="PKX82_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOF51762.1"
                     /translation="QTTTSSIHIHGCFGNFDKWQGAQWIVRELLQRELAQELDSWAFV
                     GDSGNDQAMFEHFTHSVGVANIARYAPQMQHLPRYVAPSERGAGFAEVARAILEARGA
                     "
     gene            306..1487
                     /locus_tag="PKX82_05610"
     CDS             306..1487
                     /locus_tag="PKX82_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF51763.1"
                     /translation="MSVPDTPHARLGLLSIFGSTFLELIGYFMLSPLLLLRLKGADVS
                     TTVAGLFVATGWLGIFLMTPFASTVTHRFGRRPCMWLAGALLTVCAVLFTLTQQLALW
                     FALNLLSGIAMSLRWVLAEALIAEFCPPEQRGRYVGIFQTLVSTTFIIGPATMAWMGT
                     DNPLTLWTIIAFSTLGFLWTLAIPRVPPAHDTDSAHVGLQGLWQALRAHPIIMLAGFV
                     GGFFETGVTSILPLYGLALGLTASAAAVMVSVSGLGGVVLMMPAGMVADRFADQARGR
                     RTLMVFFASVMLAATCVLPFVATIGWLAWPIVFLWGGAGGSLYTMAMTDIGAREKGIT
                     LVNSTAVLVLTYTLGGLMASGMSGALIDWSPTVGFPLVLITVAAMGLIALVRSRNAAN
                     L"
     gene            complement(1499..2077)
                     /locus_tag="PKX82_05615"
     CDS             complement(1499..2077)
                     /locus_tag="PKX82_05615"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HOF51764.1"
                     /translation="MKRRQFAGRLAVASALVCGVAGVSAAESAAEQRAEIQKMRSTTL
                     ERLYKVHPAARSEIQKAAGYAVFSNVGINLILLSAAGGSGVAHDNRSGQDTYMRMVSG
                     GFGIGLGVKDFRGVFVFSTTDKLQQFVNSGWEASAQADAAAKAGSKGGAVAGAITVAP
                     GVSLYQLTENGLALQATIQGTKYFKNDDLNAK"
     gene            complement(2092..3135)
                     /locus_tag="PKX82_05620"
     CDS             complement(2092..3135)
                     /locus_tag="PKX82_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOF51765.1"
                     /translation="MKTFYNHLHAQHQGKVEMFRGALVPCFEVPARADHVLAELQRRK
                     LGTVLQPQAFDDSAITAIHSPRYLNFLATAWDQWLALDPTNVDKDILPSVWPTRTFRT
                     DIEPDNFAAKVGLYSYDAGSPFTAGTWVAARAGADCALSAAQAVLNGERAAFALSRPP
                     GHHAGMDFFGGYCFLNNAALAAQHLRNNGLKKVAVLDIDYHHGNGTQAIFYDRPDVFF
                     ASIHGDPRTEYPFYLGHADECGAGAGLGANLNLPLPRGTDFAAWSQALETALAAIQTF
                     GADALVVSLGMDTFAGDPISGFTLRSDDYLRVGARLAKLGLPTVLVFEGGYAVAEVGI
                     NAVNVLESFSGSV"
     gene            complement(3148..4110)
                     /locus_tag="PKX82_05625"
     CDS             complement(3148..4110)
                     /locus_tag="PKX82_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOF51766.1"
                     /translation="MQRRHLVQLAATALAAPLLDAHAQAFPVKPIKLVIAFPAGGPTD
                     ITMRALADAAGKILGQPVVVENKPGAGGTMPAQQLQSSPPDGYTVAQIPLGVFRLPYT
                     TKINWDPVKDISYVLNVTGYAFGLVVPVDSALRTWTHFVAWAKANPGLLSYGSTGTLT
                     SPHLTMELIAQKLGLQLLHVPYKGSADLMQAILGGQIMAAADSTGFAPQVAAGKLRVL
                     NTWGAERLAKFPDAPTLKELGLDMVQNSPFGIGAPKGTPPAVIKRLHDAFKQAMEQES
                     YVTALARYDMVPMYMNSASYLKFAQDTFAREKALVEKLGLMRQP"
     gene            complement(4192..5688)
                     /locus_tag="PKX82_05630"
     CDS             complement(4192..5688)
                     /locus_tag="PKX82_05630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOF51767.1"
                     /translation="MNTTTNSLVQKLWNYCNVLRDDGMSYGDYVEQLTYLLFLKMADE
                     RSAPPYNQPSIVPAAYAWPTLLAKDGDELFDHYRHALEKLGQEKGTLGLIFGKAQNKF
                     QDPAKLRRVIVDLIGAETWTVLGADVKGDAYEGLLEKNAQDTKSGAGQYFTPRALIQA
                     MVDCIAPQPGERITDPACGTGGFLFTAHNYITAHNKSLTRDQLKHLKEKAFTGYELVQ
                     GTARVCAMNMMLHGIGSEKQVPVVVGDALAADPGERFEVVLANPPFGKKSSTVIVGED
                     GRTSTEKDTIERDDFWATTSNKQLNFVQHIKTLLATHGRAAVVLPDNVLFEGGAGETI
                     RKKLLHECDVHTLLRLPTGLFYAQGVKANVVFFEKKGASETPWTKQLWIYDLRTNKHF
                     TLKTSPLTRADLNEFVDLYQAGNRHQRQATWSPENPDGRWRAYSYDELVARDKTSLDI
                     FWLKDDSLADSDNLPAPGVIALEIVEDLQAALEQFKLIAGDMAGEAIE"
     gene            complement(5695..>6275)
                     /locus_tag="PKX82_05635"
     CDS             complement(5695..>6275)
                     /locus_tag="PKX82_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51768.1"
                     /translation="AQELGISVSEAHACIKRGLRAGLLVRNIGELEAPAADGLQDSNT
                     IHRVARRKRVRTGAQNDAVFDNPVRPHSKQLADFALFGAKYAFVADKVPMTIGVPTSH
                     SAPAFAGVFAPGSDDFVWPHPNGTVRGIGVEPLHPSVPFAAMQDAKLYEMLALFDALR
                     VGRARERGMAHKRLPLLIYPDGPKPIEKPLQG"
BASE COUNT         1090 a         1993 c         1922 g         1270 t
ORIGIN      
        1 caaacgacca ccagcagcat ccatatccac ggctgcttcg gcaatttcga caaatggcag
       61 ggcgcgcagt ggatcgtgcg cgaactcctg cagcgcgaac tggcgcagga gctggacagc
      121 tgggcctttg tcggcgattc gggcaacgac caggccatgt ttgaacactt cacccacagc
      181 gtaggcgtag ccaatattgc gcgctatgca ccgcagatgc agcacctgcc gcgctacgtg
      241 gcgcccagcg agcgcggcgc cggctttgcc gaagtggcac gcgccattct ggaggcccgc
      301 ggcgcatgag cgtacccgac acgccccacg cgcggctggg actactcagc atttttggct
      361 ccacctttct ggagctgatc ggctacttca tgctctcccc tttgctgctg ttgcggctca
      421 aaggggcgga tgtgtccacc acggtggccg ggctgttcgt ggccaccgga tggctgggca
      481 tttttctcat gacgccgttt gcctccacgg tcacccaccg cttcgggcgg cggccctgca
      541 tgtggctggc cggtgcgctg ctgaccgtct gcgccgtgct gttcacactc acccagcagc
      601 tggcgctgtg gtttgcactg aacctgctct caggcattgc catgtcattg cgctgggtgc
      661 tggccgaagc cctgattgcg gagttttgcc cgcccgaaca gcgcgggcgc tacgtgggca
      721 tcttccagac cctggtcagc accaccttca tcatcggccc ggccaccatg gcctggatgg
      781 gcaccgacaa ccccctgacc ctgtggacca tcatcgcctt ctccaccctg ggatttctct
      841 ggacgctggc gattccgcgg gtccctcccg cgcacgacac ggactctgcc catgtgggat
      901 tgcagggcct gtggcaggcc ctgcgtgcgc accccatcat catgctggcg ggttttgtcg
      961 gcggattctt tgaaactggt gtcacctcca tcctgccgct gtacggactc gcgctcggtc
     1021 tcacggcatc ggccgcggcc gtcatggtgt ccgtcagtgg gctgggcggc gtggtgctga
     1081 tgatgcccgc cggcatggtg gccgaccgct ttgccgacca ggcccggggc cggcgcacgc
     1141 tcatggtgtt ctttgcgtcc gtgatgctgg ccgccacctg tgttctgccc tttgtcgcca
     1201 ccattggctg gctggcctgg cccatcgtgt ttctgtgggg tggcgccggc ggctcgctgt
     1261 acaccatggc catgaccgac atcggcgcgc gcgaaaaagg catcaccctg gtcaacagca
     1321 ccgcggtgct ggtgctgacc tacacgctgg gcggtctgat ggcgtctggc atgtcgggcg
     1381 cactcatcga ctggtcgcct accgtgggct ttccccttgt gctgattaca gtagccgcta
     1441 tgggtttgat agcactggtg cgctcacgca acgcggcgaa cctttagccc cgcacgccct
     1501 atttcgcgtt caggtcgtcg ttcttgaagt acttggtacc ctggatcgtg gcctgcaagg
     1561 ccaggccgtt ctcggtcagc tggtagagac tcacaccagg cgccacggtg atggcgccgg
     1621 ccacagcgcc ccccttgctg cccgccttgg ctgctgcatc ggcctgggca ctggcctccc
     1681 agccgctgtt gacgaactgc tggagcttgt cggtggtgga gaacacgaac acgccgcgaa
     1741 aatccttcac acccagccca atgccaaagc cgccagacac catgcgcatg taagtgtcct
     1801 ggccgctgcg gttgtcatgc gctacgccag agccgcccgc ggccgacagc aggatcaggt
     1861 tgatccccac attgctgaac accgcataac ccgcagcctt ttgtatctcg gagcgggcgg
     1921 ccgggtgcac cttgtacaag cgctccagcg tggtgctgcg catcttctgg atttcagcgc
     1981 gctgctcggc ggcggactct gcggcgctga ctcccgccac gccacacacc agagcagatg
     2041 ccacggccag ccggcccgca aattgacgac gtttcatgac cactcctcct gttagaccga
     2101 cccggaaaaa ctttccagca cattgacggc attgatgccc acttcggcca cagcatagcc
     2161 gccctcaaac accagcaccg tgggcaatcc cagcttcgcc agtcgcgcac cgacccgcag
     2221 gtagtcatcg ctgcgcagcg tgaagcccga gatcgggtcg cccgcaaagg tgtccatgcc
     2281 caacgacacc accaaggcat ccgcaccgaa tgtttgaatc gcagccaaag cggtttccag
     2341 cgcctgcgac caggcagcga agtcggtgcc gcgcggtagc ggcagattca ggttggcgcc
     2401 cagccccgcg cccgcaccac actcatctgc atggcccagg tagaacgggt actcggtgcg
     2461 cggatcgcca tggatgctgg caaagaacac atcgggccgg tcgtaaaaaa tggcctgcgt
     2521 gccattgcca tggtggtagt cgatgtccag cacggcgact ttcttcaggc cgttgttgcg
     2581 caagtgctgc gcagccagcg cggcgttgtt cagaaagcag taaccaccaa agaagtccat
     2641 gcccgcgtgg tggcccggcg ggcggctcaa ggcaaaagct gcgcgctcgc cattcagaac
     2701 cgcctgcgct gcgctcagcg cgcagtctgc gcccgcccgc gcagccaccc aggtgcccgc
     2761 cgtaaagggc gagccggcgt cgtacgaata caggcccacc ttggcggcaa aattgtcggg
     2821 ctctatgtcg gtgcggaagg tgcgtgtggg ccagacggag ggcaggatgt ccttgtccac
     2881 attggtgggg tccagcgcca gccactggtc ccaggcggtg gccagaaagt tcaagtagcg
     2941 cgggctgtgg atggcggtga tggcgctgtc gtcaaacgcc tgcggctgca gcaccgtgcc
     3001 cagcttgcgc ctttgcagct cggccagcac atggtccgcg cgggcgggca cctcgaagca
     3061 cggcaccagc gcgccgcgga acatctcgac cttgccctgg tgctgcgcgt gcaggtggtt
     3121 gtagaaggtt ttcatgccaa tgctgcatca gggctggcgc atcaggccca gcttttcgac
     3181 cagggccttt tccctggcaa acgtgtcctg cgcaaacttg aggtagctgg ccgagttcat
     3241 gtacatcggc accatgtcgt agcgtgccag cgcggtcaca tagctctcct gctccatggc
     3301 ctgcttgaag gcgtcgtgca ggcgcttgat gaccgcgggc ggtgtgcctt tgggcgcacc
     3361 gatgccaaac ggcgagttct gcaccatgtc cagccccagt tccttgagcg tgggcgcatc
     3421 cgggaacttg gccagccgct cagcacccca agtattgagc acccgcagct tgccagccgc
     3481 cacctgtgga gcaaagccgg tggagtcggc cgccgccatg atctgcccgc ccagaatggc
     3541 ctgcatcagg tcggcactgc ccttgtacgg cacatgcaga agttgcaggc ccagcttctg
     3601 cgcaatcagc tccatggtca ggtgcgggct ggtcagggtg ccggtggatc cgtaagacaa
     3661 caaacctggg ttggccttgg cccaagccac aaagtgggtc caggtgcgca gcgcggaatc
     3721 caccggcacg acgaggccaa aagcatagcc ggtaacgttg agcacatagc tgatgtcctt
     3781 gaccgggtcc cagttgatct tggtggtgta cggcaggcgg aacacgccca gcggaatctg
     3841 ggcaactgtg tagccgtcgg gcggcgaaga ttgcagttgc tgggccggca tggtgccccc
     3901 tgcgcccggc ttgttttcca ctaccaccgg ttggcccagg atcttgcccg ccgcgtcggc
     3961 caaggcccgc atagtgatgt ctgtcggccc gcccgccggg aacgcaatga ccagtttgat
     4021 gggcttgacc ggaaatgcct gcgcatgggc atccagcagg ggtgcggcga gtgcggtggc
     4081 tgcgagttgt accagatgac ggcgttgcat agtgcgctcc aaaaaatgag gtcaatacgc
     4141 gctattacac cggactttac agtgagttgc catgcggtaa acccgtgcaa cctattcgat
     4201 ggcctccccg gccatatcgc cggcaatcag cttgaactgc tccagcgccg cctgcagatc
     4261 ttccacaatt tccagcgcaa tcacgcccgg ggctggcaga ttgtcgctgt cggccaatga
     4321 gtcatctttg agccagaaga tgtccagact ggttttgtcc cgcgcaacca gttcgtcgta
     4381 gctgtaggcg cgccaacggc cgtccgggtt ctcgggcgac cacgtggctt ggcgctggtg
     4441 gcggttgcct gcctgataca ggtctacaaa ctcgttcaag tccgcgcggg tgagcgggct
     4501 ggttttgagc gtgaagtgct tgttggtacg caggtcgtaa atccacagct gcttcgtcca
     4561 cggggtttcg ctcgctccct tcttctcaaa gaagaccaca ttggccttca caccctgggc
     4621 ataaaacagg ccggtgggca ggcgtaggag cgtgtgcaca tcgcattcgt gcagaagctt
     4681 cttgcggatg gtttcacccg cgccgccttc aaacaacacg ttgtcgggca acaccacggc
     4741 cgcgcggccg tgggtggcca gcagggtctt gatgtgctgc acaaagttga gctgcttgtt
     4801 tgatgtggtg gcccagaagt catcacgctc gatggtgtct ttttcggtgc tggtgcggcc
     4861 atcttctccc acgatgaccg tgctgctctt cttgccaaac ggcggattgg ccagcaccac
     4921 ttcaaaacgc tcgccggggt cggcggccag cgcatcgccc accaccacgg gcacctgctt
     4981 ctcagaacca atgccgtgca gcatcatgtt catggcgcac acgcgtgccg tgccctgtac
     5041 cagctcgtaa ccggtgaagg ctttttcttt caggtgcttg agctggtcgc gcgtgaggct
     5101 cttgttgtgg gccgtaatgt agttgtgcgc ggtgaacaag aagccgccgg tgccacaggc
     5161 ggggtcggta atgcgctcgc cgggttgcgg ggcaatgcaa tccaccatcg cctgaatcaa
     5221 cgcgcggggc gtgaagtatt ggcccgcgcc gctcttggtg tcttgcgcgt tcttttccag
     5281 caggccttcg taagcatctc ccttcacgtc cgcacccagc accgtccagg tttcggcacc
     5341 aatcaaatcg acgatcacgc ggcgcagctt ggccgggtcc tggaacttgt tttgcgcctt
     5401 gccaaagatc aggcccagcg tgcccttttc ttggcccagc ttttccagcg cgtggcggta
     5461 gtggtcaaac agctcgtcgc cgtctttggc cagcagcgtg ggccaggcat aggcggcggg
     5521 cacgatgctg ggctggttgt agggcggtgc gctgcgttcg tcggccatct tgaggaacag
     5581 caggtaggtg agttgctcca catagtcgcc atagctcatg ccgtcgtcgc gcagcacgtt
     5641 gcagtagttc cagagcttct ggacgaggga gttggtggtg gtgttcatat ttttctatcc
     5701 ctgcaaaggt ttttctattg gcttggggcc atctgggtaa atcaaaagcg gcaaacgctt
     5761 gtgcgccata ccacgttcgc gcgcacggcc gacgcgtaag gcgtcaaaca aggccaacat
     5821 ttcatacaac ttggcatcct gcatcgctgc gaaaggcact gaaggatgca atggctcaac
     5881 gccaattcca cgcactgtgc cgttgggatg tggccacaca aaatcatctg agcctggtgc
     5941 aaagacacca gcgaacgccg gcgcgctgtg gctggtcggt acacctatcg tcattggaac
     6001 cttgtcggct acgaaggcgt acttcgctcc aaaaagcgca aagtcagcca actgttttga
     6061 gtgtgggcgt acgggattgt caaataccgc gtcattctgc gcgccggtcc gcacgcgctt
     6121 tctgcgcgcg acacggtgga tcgtatttga gtcttgtagc ccatctgcgg ccggagcttc
     6181 caattcccca atgtttcgga ccaagagccc ggcccgcagg ccacgcttaa tgcaggcatg
     6241 tgcctccgag acgctgatac ccaactcttg cgcca
//
LOCUS       DAOSJM010000118         6248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11328_length_6248_cov_3.485225, whole genome shotgun sequence.
ACCESSION   DAOSJM010000118 DAOSJM010000000
VERSION     DAOSJM010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11328_length_6248_cov_3.485225"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..920
                     /locus_tag="PKX82_05640"
     CDS             <1..920
                     /locus_tag="PKX82_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HOF51769.1"
                     /translation="TLSGANPFASGILSRVAESDPACTSRLTLWSNVLHLIAQKPWFG
                     WGWGELDYAHFITLYPGERFCEILDNAHNLPLHLAVELGIPIAATFCGGCAWLIWRNK
                     PWAEKDPTRQMAWGVLAVIGLHSMLEYPLWYGPFQLAVVLCFWLLWRNPKADVETAQT
                     SRLQGPSVARAATVAAAVALLALCTYVAWDYWRISQIYLPPAARHAAYQGDTLAKVQG
                     SRLFRDQVQFAELTLTPLDGDNAVHIHALAKAMLHFSPEPRVVEKVIESAVMLHRDDE
                     ALFYLQRYKAAFPAQHARWSTALKVPKQD"
     gene            complement(917..1408)
                     /gene="moaC"
                     /locus_tag="PKX82_05645"
     CDS             complement(917..1408)
                     /gene="moaC"
                     /locus_tag="PKX82_05645"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOF51770.1"
                     /translation="MSTLTHFDAQGQAHMVDVSAKNNTHRIAVARGRIEMLPATLAMI
                     EQGNAKKGDVLGIARIAGIQGAKKTSDLIPLCHPIALTRVAMDFEVVPASGATAAHVV
                     CSATAETVGPTGVEMEALTAVQVALLTIYDMCKAVDRGMTITDVKLLEKHGGKSGSFV
                     ATR"
     gene            1468..2241
                     /gene="tfpZ"
                     /locus_tag="PKX82_05650"
     CDS             1468..2241
                     /gene="tfpZ"
                     /locus_tag="PKX82_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfpX/TfpZ family type IV pilin accessory
                     protein"
                     /protein_id="HOF51771.1"
                     /translation="MFSDTPSFWGDRFKASALHLGLSLCIAALAALLVFAVWYPNPYR
                     EISGGRELFLLLVAVDVILGPLITLAVFNRKKPHRELRRDLMVVGLIQLAALGYGLWT
                     VAVARPVHMVFEYNHFRVVHAIDVPPELLSQTPAGVEALPLTGPTLLGLRPFKDDKES
                     MDATMAALQGLTLSSRPDLWQPYEKSVPEVLKVARPASELKARFVNQATEIDRVLTQA
                     GRTAENTVYVPMAGRKSFWTVFLDASSAQVVAAMPLDSF"
     gene            2399..3793
                     /locus_tag="PKX82_05655"
     CDS             2399..3793
                     /locus_tag="PKX82_05655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOF51772.1"
                     /translation="MTPSAERRLGDRIARELYRDPDYIDDPVTMEYVQAIWQPLLASA
                     RARGELPPELDGAYAWEILLGKDRTVNAFALPGAYLGLHLGLVSVVSSRDELASVLAH
                     ELSHVTQRHIARMMTRQSEQAPWLLGAMILGALAASKSPGAANAAIIGGQAVAAQGQL
                     NFSRDMEREADRVGYGVMTQAGYAPQAFVSMFEKLQQASRLNDSGNFPYLRSHPLTTE
                     RMADMQARIPDGAASSAGALSMEHAMVASRARVLSNGNVDALRTWAKETEPTRLSRLV
                     PAAQAGALYGAALAASKLRDFAVAQTLWTRLTVLASTDAAAGRLARLLGAELALVQGA
                     PASALGLLQNERDVGRAAVFLRAQAAVLAGKASQTTQSLQTWLADHPRDAQGWQLLAM
                     AYAAQGKTLGAIRAEAEVNVAQLDYEAALTRLKVAQDLARKGAAAGDHIEASIVDTRA
                     RQVGLLLREQAVER"
     gene            complement(3761..4114)
                     /locus_tag="PKX82_05660"
     CDS             complement(3761..4114)
                     /locus_tag="PKX82_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOF51773.1"
                     /translation="MKTLVKWLLSAAALLAVAHLYSGVAVQSFTSALIAALVIGLFNA
                     VLRPVLVVLTLPVTVVTLGLFLFVINALMFWSAAGILDGFHVQGFSAALIGSLIYTLL
                     GMVINSALDRLFPKQ"
     gene            complement(4138..4842)
                     /locus_tag="PKX82_05665"
     CDS             complement(4138..4842)
                     /locus_tag="PKX82_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOF51774.1"
                     /translation="MEFLSAPWWSALLAIVLIDLVLAGDNAIVIALAARNLPEHLKKK
                     AIVWGTVGAIAVRSVMTVGVVWLLKIPGLMLVGGLGLVWVAYKLLADQGEGEHDGPMA
                     STFWGAMKTIIVADALMGVDNVLGVAGAAHGAFDLVIIGLLVSVPIVVFGSSVVLKLV
                     DRFPVIIQLGSAVLAFTAAKMVVSEPVLSNLFGASGAVTTDMQTAARWGTYAVAVAGV
                     LLAGWWSQRSTKPAGL"
     gene            5136..5567
                     /locus_tag="PKX82_05670"
     CDS             5136..5567
                     /locus_tag="PKX82_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HOF51775.1"
                     /translation="MAGIHITDIESAINYWRARAPSPDGVSLPAPTRALAEVYALLIY
                     YHQTEADEFTMPAKALDAWMEWYRSTPDTPCIAICSTSQGDEECKGCGRSFSEVQLWT
                     EMRPAEKRATWRRITQEGTSWRFNKYAERAAEGVPDLLPNS"
     gene            complement(5611..6171)
                     /locus_tag="PKX82_05675"
     CDS             complement(5611..6171)
                     /locus_tag="PKX82_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HOF51776.1"
                     /translation="MAIKSTIFKANLQIADIDHGYYADHALTLARHPSETDERMMVRL
                     VALALNAYKLQSECNGDGTLAFGAGLSDVEDPDVSLTDFTGHKRLWIEVGQPEEKPIS
                     KACNKADAVLLYCFAHSADIWWKGIQNKLTRLDKLQVWRIPSATSQALAQLAQRSMQL
                     QATIQENVLTLGDATNAVDIELIRWK"
BASE COUNT         1162 a         1889 c         1986 g         1211 t
ORIGIN      
        1 caacgctttc gggagcaaat ccatttgcaa gtggcatttt gagtcgcgtt gcagaaagtg
       61 acccagcctg cactagccgc ctcaccctct ggtccaacgt actccacctc atcgctcaaa
      121 agccgtggtt cggctggggt tggggcgagc tggactacgc gcatttcatc accctgtacc
      181 ctggcgagcg gttttgtgag attctggata acgcgcacaa cctcccgttg cacttggcgg
      241 tggagttggg tattccaatc gccgccacat tctgcggtgg ttgtgcctgg ctgatctggc
      301 gcaacaagcc ttgggctgag aaggatccca cgcgccagat ggcgtggggt gtgctggcgg
      361 tgattgggtt gcacagcatg ctggagtacc cgctgtggta cgggccgttc cagttggcgg
      421 tggtgctgtg cttctggctg ctgtggcgaa acccaaaggc ggatgttgag acagcgcaga
      481 cttcccggtt gcaagggcct tcagtggctc gtgctgcaac agttgctgcg gctgtggcct
      541 tgctcgcgct ttgtacctat gtcgcctggg actactggcg tatcagccag atctatctgc
      601 cgccagctgc gcgccatgcg gcgtaccagg gagacaccct ggccaaagtg cagggctcac
      661 ggttattcag ggatcaggtc cagtttgcag aactcaccct cacgccactg gacggggata
      721 acgccgtcca tattcatgcg ctggccaaag cgatgctgca tttttcaccc gaaccgcgcg
      781 tggtggaaaa agtgattgaa agcgctgtga tgctccaccg ggatgacgaa gccttgttct
      841 acttgcagcg ctacaaggcg gcgtttccgg cgcagcatgc gcgctggagc actgcactca
      901 aggtgcccaa gcaagactag cgtgtagcca caaagctgcc cgacttgccg ccgtgctttt
      961 ccagcagctt tacatcggta atggtcatgc cacggtccac ggccttgcac atgtcgtaga
     1021 tggtgagcag ggcgacctgc accgcagtga gtgcctccat ttccacacca gtggggccta
     1081 ccgtctcggc agtggccgag cagaccacat gtgcggcagt cgctccagag gcgggcacga
     1141 cctcaaaatc catggccacg cgggtgagtg caatcgggtg gcacaagggg atcaggtcgc
     1201 tggttttctt tgcgccctga atgccggcaa tgcgcgcaat gccgagcaca tcacctttct
     1261 tggcgttgcc ctgctcgatc atggcgagcg tggcgggaag catctcgatg cgcccgcgcg
     1321 ccacggcgat gcggtgggta ttgtttttgg cggacacatc caccatatgg gcctggccct
     1381 gtgcgtcaaa gtgggtaagg gtactcataa tgtccacttt acctgacctt cacatcaagt
     1441 accgcgagtt aatggcatcc tatgaccatg ttttctgata ctccctcgtt ctggggcgac
     1501 cggttcaagg ccagcgccct ccatttgggc ttgagcctgt gcatcgcggc gctggcggca
     1561 ttgctggtgt ttgccgtctg gtaccccaac ccttaccgcg aaatctcggg tggacgcgaa
     1621 ctcttcctgc tgctggtggc ggtggatgtc attttggggc cgctgatcac gctggcggtg
     1681 ttcaaccgga agaagccaca tagggagttg cggcgtgatc tgatggtggt gggcctgatc
     1741 cagcttgccg cgcttggcta tggtttgtgg acggtcgcgg tggcacgccc ggtgcacatg
     1801 gtgtttgaat acaaccactt ccgggtggtc catgccattg acgtgccacc ggagttgctg
     1861 tcgcagacgc ctgcaggggt ggaggccttg ccgctcaccg gccccacgtt gctgggcttg
     1921 cgtcccttca aagacgacaa ggagagcatg gatgccacga tggccgccct gcaggggttg
     1981 accttgagtt cccgtcccga cctgtggcag ccctatgaaa aatcggtgcc cgaggtattg
     2041 aaggtggcgc gtcccgcctc tgaattgaag gcccgctttg tgaaccaggc cacagagatt
     2101 gatcgcgtcc tcacccaggc aggccgcact gcggaaaaca cggtgtatgt cccgatggcg
     2161 ggacgcaaat cgttctggac cgtgtttctc gatgcctcca gcgcacaggt ggtggccgcc
     2221 atgccgctgg attccttttg acgccgaatt taagtaaaaa gtgcctctag cccccgtcaa
     2281 atatgcgcag gcagctatta tttgtatagc gctgctggcg gcggcaaatg ccctgccgca
     2341 gaccgaaccg gcccgcgtaa gtggcagcag tctgcccaat ctgggcgatg gcagcgacat
     2401 gacgcccagc gccgagcgcc gcctgggaga tcgcattgca cgcgagttgt accgggaccc
     2461 ggactacatc gacgatccgg tcaccatgga atatgtgcag gcgatctggc agcctctgct
     2521 ggcgtcagcg cgtgcccggg gtgagttgcc gccggagctg gatggcgcct acgcctggga
     2581 aatcctgctg ggcaaggacc gcacggtcaa tgcgtttgcc ctgccaggtg cctacctggg
     2641 cctgcatctg gggctggtga gtgtggtgtc cagccgggac gaactggctt cggtgctggc
     2701 ccatgaactc agccacgtga cccagcgcca catcgcccgc atgatgaccc gccagagcga
     2761 gcaggcgccc tggctgctgg gcgccatgat tttgggtgcc ctggcggcca gcaagagtcc
     2821 gggagcggcc aatgccgcga tcattggcgg tcaggccgtg gcggcgcagg ggcagttgaa
     2881 cttctctcgg gacatggagc gcgaggcgga tcgggtgggc tacggtgtga tgacgcaggc
     2941 gggctacgcg ccccaagcct ttgtatccat gtttgagaag ctgcaacagg cttcccggct
     3001 caacgacagt ggcaactttc cctacctgcg cagccacccc ttgaccactg agcgcatggc
     3061 cgatatgcag gcgcgtattc ctgacggcgc ggcgagctcc gcaggcgcgt tgtcgatgga
     3121 gcacgccatg gttgcgtcgc gcgcccgggt gctgtccaac ggcaatgtgg atgcgctacg
     3181 cacctgggcc aaagagaccg agcccactcg cctctcccgc ctggtgcccg cggcccaggc
     3241 cggggccctg tatggcgccg cgctggcggc cagcaaactg cgcgattttg cggtagcgca
     3301 aaccctgtgg acgcgcttga ctgtcctggc ttccacggat gcagccgccg gtcgtctggc
     3361 ccgtttgctg ggggcagagc tggcgttggt gcagggagcg ccggccagcg cgttgggtct
     3421 gttgcagaat gagcgcgacg tcgggcgtgc cgccgtgttt ctgcgggcgc aagcggcagt
     3481 tctggcgggc aaggcgtcgc aaaccaccca gagtttgcag acctggctgg cggaccatcc
     3541 gcgcgatgcc cagggctggc aattgctggc catggcctat gcggcacagg gcaagacgct
     3601 gggcgccatc cgggccgagg cggaggtcaa tgtcgcgcaa ctcgattacg aggccgccct
     3661 gacacggttg aaagtggccc aggacctggc gcgcaagggc gcagccgcag gggaccacat
     3721 cgaggcgtcg atcgtggaca cccgggcacg ccaggtgggg ttattgcttc gggaacaggc
     3781 ggtcgagcgc tgagttgatg accatgccca gcagtgtgta gatcagcgag ccgatcagtg
     3841 ccgcgctgaa tccctgcaca tggaagccat ccagaatccc ggcggcggac cagaacatca
     3901 gcgcgttgat gacaaacagg aacaggccca gtgtcaccac ggtcacaggc agcgtcagca
     3961 ccaccagtac cgggcgcagc acggcattga acaggccgat caccagtgcg gcaatcagtg
     4021 cggaagtaaa actctgcacc gcaacgccgc tgtagagatg ggctacagcc aagagggcgg
     4081 ctgcgctgag cagccatttc acaagagtct tcatgatcgc agcatagcac ctgccaatta
     4141 cagcccggcc ggttttgtgg agcgctggct ccaccagcct gccagcagga cgccggccac
     4201 cgcaacggcg taggtgcccc agcgggcagc ggtctgcatg tcggtagtga ccgcaccgga
     4261 agcgccgaac aggttcgaca ggaccggctc gctcacgacc atcttggccg cggtaaacgc
     4321 cagcacggca gaacccagct ggatgatgac cggaaagcgg tccaccagtt tgagcaccac
     4381 cgagctgcca aacaccacaa tcggcacgct gaccagcagg ccaataatga ccaggtcaaa
     4441 cgctccgtgc gctgcacctg caacgccgag cacgttgtcc acccccatca gggcatcggc
     4501 cacgatgatg gttttcatgg cgccccagaa ggtgctggcc atcgggccgt cgtgctcgcc
     4561 ttcgccctgg tctgccagca gcttgtaggc gacccatacc agccccagac cacccacgag
     4621 catcaagccg gggattttca gcagccacac cacgcccacg gtcatgaccg agcgcacggc
     4681 aatggcgccc accgtacccc acacaatggc tttcttcttg aggtgttcag gcaggttgcg
     4741 cgctgccagc gcgatgacga tggcattgtc accggccaga acgaggtcga tgaggacgat
     4801 ggccagaagg gcagaccacc agggtgcaga tagaaattcc atgtcaattc cagttggggg
     4861 ttaccagggg tattgcgtac cgcaagcgca cttcgaccga gcctgtttcg ggctccaatc
     4921 gaaggtcttg ctcagccagt gggtgggacc ctgtggccct gcaagccgga agcacgggct
     4981 tcgtattgac ggcttgcaga tacccgctgc gtggcaacgg gcgggagcta ctccccttcg
     5041 cgaagcattg caccacaaag gcaacaggcc ttccattcgg agggtttatt gtgccctctg
     5101 gtccacgcta tgatgcgcct cccctacagg actctatggc aggcatccac atcaccgaca
     5161 ttgaatcggc catcaactac tggcgcgcgc gcgcgccctc gcccgatggc gtctcgctgc
     5221 ctgcgcctac aagggcgttg gcggaggtgt atgcgctgct gatctattac caccagacgg
     5281 aagcggacga gttcacgatg cccgccaagg cgctggatgc ctggatggag tggtaccgca
     5341 gcacacccga tacgccctgc attgccattt gctccaccag ccagggcgat gaagaatgca
     5401 agggctgtgg ccgcagtttc tcggaagtgc agttgtggac cgagatgcga cctgcagaaa
     5461 aacgtgccac ctggcgccgc attacgcagg aaggcacgtc ctggcggttc aataaatatg
     5521 ccgagcgtgc cgccgaaggt gtgccagatt tgctaccaaa ttcatagctg cttgtgcacg
     5581 ctccgtgcgg gctaaaggcc aatttggcta ctatttccag cgaatcagtt caatgtccac
     5641 cgcgttggtg gcatcgccca gtgtgagcac gttttcctgg atggtggcct gcaactgcat
     5701 gctgcgctgg gccagttggg ccagtgcctg cgaggtggca gagggaatgc gccagacctg
     5761 cagtttgtcc agccgcgtga gcttgttctg gatgcctttc caccagatgt cggcggagtg
     5821 ggcaaagcag tacagcagca ccgcatcggc cttgttgcag gccttgctga tgggtttctc
     5881 ctcgggctgg ccgacctcga tccacaggcg cttgtggccg gtgaagtcgg tcagcgacac
     5941 gtccgggtct tccacatccg acaggccggc accaaatgcc agcgtgccgt cgccattgca
     6001 ctcgctttgc agcttgtagg cgttgagcgc cagcgccacc aggcgcacca tcatgcgctc
     6061 gtcggtttcg ctggggtggc gcgccagggt cagcgcatgg tcggcgtagt agccgtggtc
     6121 gatgtcggcg atctgcaggt tcgccttgaa gatggtggat ttgatggcca tggatgtatc
     6181 aaaaacaaga gctgccagcg caagtgggta cttgctttgg cagctccaga ggttttaagt
     6241 acccacgc
//
LOCUS       DAOSJM010000119         6242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11346_length_6242_cov_3.609504, whole genome shotgun sequence.
ACCESSION   DAOSJM010000119 DAOSJM010000000
VERSION     DAOSJM010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6242
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11346_length_6242_cov_3.609504"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="PKX82_05680"
     CDS             <1..775
                     /locus_tag="PKX82_05680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF51777.1"
                     /translation="LIAFNVLLGRVVLKPLNRLASAIELVQHADFVPQDLPQGGKDEI
                     GHLMRLFQAMAMHIWASRNELEARVAERTAALEQSEQRLRHMAQHDPLTGLANRALFT
                     DRLQQALAAAHRDHTQVALLFLDLDNFKPVNDQYGHAMGDELLKVAASRMQGCLRDSD
                     TVARIGGDEFVLLLRSLGAPASHTALAVAEKIRAALSQPFELAGHALQISCSIGVALY
                     PDHALDDIALAHHADQAMYQAKLAGRNRVVLCESPVQDA"
     gene            complement(779..2047)
                     /gene="glgC"
                     /locus_tag="PKX82_05685"
     CDS             complement(779..2047)
                     /gene="glgC"
                     /locus_tag="PKX82_05685"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005674312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOF51778.1"
                     /translation="MTLDSKNHLPERTYALVLAGGRGSRLHQLTDWRAKPAVPFAGNL
                     SIIDFALSNCVNSGLRHIAVLTQYKAQSLIRHIERGWSFLAASLGEYVDVVPAQQQHG
                     ESWYSGTANAVYQNLRIIDEAQPDLVLVLGGDHIYKMDYSIMLAEHVARGAAMTVACL
                     EVPVEQASEFGVMSIDADHRIVAFAEKPAQPMVLQHRPTHALASMGIYVFNAGLLVEE
                     LVRDAADPASSHDFGKDIIPSLLARHRVFAHSFKDSCVNMVDTTPYWRDVGTVDAYWE
                     ANMDLTSVVPELNLYDDGWPILSLQRQLAPAKFVFDEAGRRGEAHNSLVSSGCIVSGA
                     NVRHSILFSKVKVREGSVIEDSVVLPNVTIGKGVSLRRTIIDKHCKLPDGFVAGLDAA
                     QDRTRGFHVSDNGITLITPEMLGQRIHRVT"
     gene            complement(2139..2399)
                     /locus_tag="PKX82_05690"
     CDS             complement(2139..2399)
                     /locus_tag="PKX82_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HOF51779.1"
                     /translation="MTPNHIWLILLAATGVTFWLGESGLSGSAGMAPVLVMFALAFVK
                     ALLVMRDFMELRHAPALWRWLLQGWLVLVTTGIVLAYWIGQR"
     gene            complement(2396..2977)
                     /locus_tag="PKX82_05695"
     CDS             complement(2396..2977)
                     /locus_tag="PKX82_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HOF51780.1"
                     /translation="MTLRNTMPDPPRLVGDLAVWLIIVAEMLAFCILFLSYAFARAKD
                     VALFNASQSTLDLHSGAINTVLLITGSWCVVRAVQAVRTNDSSSGARWLVAALVCGAG
                     FVTLKVLEFSAKADAGIDLSTNTFFMFYLLLTGFHFLHVIAAMLFLTLLLFKTLQGAY
                     GSSDSHALESGAAFWHMVDLLWIVLFPLVYVMR"
     gene            complement(3044..3484)
                     /locus_tag="PKX82_05700"
     CDS             complement(3044..3484)
                     /locus_tag="PKX82_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51781.1"
                     /translation="MKQTKPYGRAFQASDSATSNQHLMPALSVDIPLPISAEMGHERP
                     ALDIGEQFSIAIPATEPMPLPIRMLAYAATAVVATAGYIWIRDSLFAQNLITMVTTKE
                     TVMVCLQGSMQYDGWRIWDRLAGNGYFVCTDWKVQTRFGNIPRF"
     regulatory      complement(3581..3670)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            3955..>6242
                     /locus_tag="PKX82_05705"
     CDS             3955..>6242
                     /locus_tag="PKX82_05705"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF51782.1"
                     /translation="MSIQLSSTPARQWLLSVATFLLALVAAFVWIAHSEQQEEEKYKA
                     RASDLAADHAQSLQRGIERALSATYAVAALVRQNNGSVHDFVAVATEMLPYYPGISAL
                     GLAPEGIICCVVPLAGNEKSIGFNQLGDSAQNKEARLARDTGQLTLAGPLNLAQGGQG
                     IVGRLPIFLTDAQGDRKFWGFTYVTLRFPLALNAARLPLLVERGYAYELWRTVPDTGE
                     HQRIVSSGEASLIDPVQRSLELPNGVWTLSVAPIQGWSDQKRWAMKVGFGMLFSALIG
                     YLTWLLYAMRLRDVHLEALVSRRTSEILTTQQHLEATVNAIPDLLFEVGLDGRYYSSH
                     SPRAELLARPAEELQGRTVNDVMPPEAARAVLDALQEANEKTWSVGVQICLPLDIGSR
                     WFELSVARKATVPGTEPRFVVLSRDITKTREAEEKIRQLAHFDALTGLPNRVLLADRC
                     HYALSTAQRNGQSVALMFLDLDHFKNVNDSLGHRIGDALLRALAGRLSASVRDQDTVS
                     RLGGDEFILVLPDTDALGAAHVAEKLMGAALEPFNIETHELTVTPSIGIAMYPTDGTD
                     FDTLSRCADAAMYHAKQRGRNHYRFFTAEMQATSERTLVLENALRRALDRNQLQLHYQ
                     PQVSLDTGAIVGAEALLRWNHPELGSISPAEFIPVAESSGLILPIGEWVLRTAVRQLR
                     QWLGAGVAPICMAVNLSAVQFRQAEFPQLIARILEEENLPAHLLELELTEGVAMTDPN
                     AAIGMMDKLHQHGVRMSIDDFGT"
BASE COUNT         1271 a         1794 c         1894 g         1283 t
ORIGIN      
        1 gctgattgct ttcaatgtgc tgctcgggcg agtggtgttg aagccactga accgtctggc
       61 cagtgccatt gagctggtgc agcacgccga ctttgtgccg caagacctgc cacagggtgg
      121 caaggatgaa atcggccacc tgatgcgcct ctttcaggcc atggccatgc acatatgggc
      181 ttcacgcaac gagctggagg cccgggtggc tgagcgcact gccgcactgg aacaaagtga
      241 gcagcggttg cgccatatgg cgcagcatga ccccctgacc ggcctggcca accgcgcgct
      301 gttcaccgac cgcctgcaac aagcgctggc tgcggcgcat cgcgatcaca cccaagtggc
      361 gctgttgttt ctggatctgg acaacttcaa gcccgtgaat gaccaatacg gccatgccat
      421 gggcgacgaa ctgctgaagg tggcggccag ccgcatgcag ggctgtctgc gcgactcgga
      481 taccgtggcg cgcattggtg gtgacgagtt cgtcctgttg ttgcgcagcc tgggcgctcc
      541 cgccagccac accgcattgg ccgtggccga gaaaattcgt gcagccctgt cacagccgtt
      601 tgaactggct ggccatgcgt tgcaaatctc ctgctccatt ggtgtggctt tgtaccccga
      661 ccacgctctg gatgacatcg ccctggccca ccacgcggat caggccatgt accaggcaaa
      721 gctggctggg cgtaaccgcg tagtgctgtg cgagagtccg gtgcaggacg cgtaaagttc
      781 aggtcacccg gtggatgcgt tgtcccagca tctctggcgt gatcagggtg atgccgttgt
      841 cactgacatg aaagccacgc gtgcggtcct gcgccgcatc cagaccggcg acaaacccgt
      901 ctggcaactt gcaatgcttg tcgatgatgg tccggcgcaa gctgacgcct tttccgatgg
      961 tcacgtttgg gagtacgacc gagtcctcga tcacactgcc ctcgcgcacc ttcactttgg
     1021 agaacagaat ggaatggcgc acattggcac cactgacgat gcaaccgctg gacaccagcg
     1081 agttgtgggc ctcgcctctg cgcccagcct catcaaacac aaacttggct ggggctagct
     1141 ggcgttgcag gctcaggatg ggccagccgt cgtcgtacag gttgagctct ggcacgaccg
     1201 aggtgaggtc catattggcc tcccagtagg cgtccaccgt gccgacgtcg cgccagtacg
     1261 gtgtggtgtc caccatattg acacagctgt ctttgaagct atgggcgaaa acgcgatgcc
     1321 gggccagcag cgatggaatg atgtccttgc cgaagtcgtg acttgacgca gggtccgctg
     1381 catcccgcac cagttcctca accagcaggc ccgcgttgaa cacgtagatg cccatgctgg
     1441 ccagtgcatg cgtgggacgg tgctgcagca ccattggctg cgcgggtttc tccgcaaaag
     1501 ccacgatgcg gtggtccgca tcgatggaca tcactccgaa ctcgctggcc tgttccacgg
     1561 gcacttccag gcaagccaca gtcattgcgg ccccgcgtgc cacatgttcg gccagcatga
     1621 tcgagtaatc catcttgtag atgtggtcac cacccagcac cagtaccagg tcgggctggg
     1681 cttcgtcgat gatgcgcagg ttttggtaca ccgcgttggc agtcccgctg taccaggact
     1741 ctccgtgttg ctgctgcgct ggcaccacat ccacgtattc gcccaggctg gcggccagaa
     1801 agctccagcc gcgttcgatg tggcgtatca ggctttgtgc cttgtactgg gtcagtaccg
     1861 cgatgtggcg caggccagag ttgacgcagt tgctgagcgc aaagtcaatg atgctcagat
     1921 tgccggcaaa agggaccgct ggtttggccc gccagtcggt cagttggtgc aagcggctgc
     1981 cacgcccacc ggcaagaacc agggcgtaag ttcgttcggg cagatggttc tttgagtcca
     2041 atgtcacggt gactttctcc tcagcgaaag atggcgcatt gaacccgcga ttgaggggcg
     2101 gatcgttgat gcagatcaat gtggctgcga tcgcccgcct agcgctggcc gatccaatag
     2161 gccagcacaa tcccggtcgt gaccagcacc agccagccct gcagcagcca gcgccacagt
     2221 gcaggcgcat ggcgcaactc catgaagtcg cgcatcacca gtagcgcttt gacaaatgcc
     2281 agtgcaaaca tgaccagcac tggcgccatg ccagcgctgc ccgacaggcc actttcgccc
     2341 agccagaaag tcacgccagt ggcagctagc agaatcagcc agatgtggtt aggtgtcatc
     2401 gcatgacata gaccaatggg aacagcacga tccacagcag gtccaccata tgccagaacg
     2461 cagcgcccga ctccagcgca tgggaatcgc tggatccgta ggcgccttgc agtgtcttga
     2521 agagcagaag cgtcaagaac agcatggccg cgatcacgtg caaaaagtga aacccggtaa
     2581 gcaggaggta gaacatgaaa aacgtgttgg tggacaggtc gatgcctgca tccgccttgg
     2641 cagaaaactc cagaaccttg agcgtcacaa agccggcacc gcacaccagt gcagccacca
     2701 gccagcgcgc gcctgacgaa gagtcgttgg tacgcactgc ttgcacggcg cgcaccacac
     2761 accagctgcc ggtgatcagc agcacggtgt tgatggcacc ggagtgcaaa tccagcgtac
     2821 tttgcgaagc attgaaaagc gccacatcct tggcgcgcgc aaaggcatag gacaaaaaca
     2881 ggatgcagaa cgccagcatc tccgcaacga tgatcaacca gaccgccaga tcgcccacca
     2941 gtctgggcgg atcgggcatg gtgttgcgta aggtcatcgg gtcattttaa gaatcagccg
     3001 cgtagtgtcc ttgaactgga acagattgag aatggcttgc cgcttaaaaa cgggggatgt
     3061 tcccaaagcg tgtttgcact ttccagtcgg tacacacgaa gtagccgttt ccagcgagcc
     3121 ggtcccaaat gcgccaacca tcgtattgca tgctgccctg cagacagacc ataacggttt
     3181 ccttggtggt caccatggtg atcaggttct gcgcaaagag gctgtccctg atccagatgt
     3241 agccggcagt ggcgaccacg gcggtcgctg cgtaggcaag catccggatg ggcaggggca
     3301 ttggttccgt ggctggtatt gcgatggaaa actgctctcc aatatcgagg gcaggccttt
     3361 catggcccat ttctgcggat atgggcagcg ggatgtcgac actcagggcc ggcatcaggt
     3421 gctggttaga agtagcgcta tcggatgctt ggaaagcacg accatagggt ttggtctgct
     3481 tcatggcacc gctccgggtg cgacatacat agctgacatg caagatcctt tcgatgtgtc
     3541 aaacgaaagg gcacgacaca aacgagcgct gccaagcagc accggcaacc ccgctatccg
     3601 cctcataaca acaaggtttg gaccggtggc tttgcgtccc tgtctctcaa caggtttgcc
     3661 caaaaagtgc atcaggttgc aaccggggtt gcgtacaaat gctgcgcgag tttatctgat
     3721 acgtgtgtaa gaacatgcac cttctcccac aaccagcctt tcaagaaaat agggttttca
     3781 aacttgctat agttttagga gctatttgcg catactccac gggggctgca agtgcttttt
     3841 gcttatatta ttttgccgtg tgcagtgtgc aaactatctc gaccggtttg tcttgtgttt
     3901 tatggggtca caacccattg ctcccggtac ggctcggagc tagactggat acgcatgtct
     3961 atccaactgt cttctacccc tgcgcggcaa tggctgttgt cggtcgcgac gtttctgcta
     4021 gcccttgtgg cggcttttgt gtggattgcc cattccgaac agcaggaaga ggaaaagtac
     4081 aaagcccggg cttctgatct ggctgcggac catgcgcagt cgctgcagcg gggcattgag
     4141 cgggcactgt ccgcgaccta tgcggttgcg gcgctggtgc ggcagaacaa cggcagcgta
     4201 catgactttg tggccgttgc aacggagatg ctgccctact accccggtat cagtgctttg
     4261 ggtctcgctc cagagggcat catttgctgt gtggttccac tggcaggcaa tgagaagagc
     4321 attggcttca atcagttggg tgacagcgcg cagaacaagg aagcccgtct ggcacgcgat
     4381 accggacagc tgacccttgc cggcccactc aatctggctc agggcgggca gggcatcgtg
     4441 gggcgattgc ccatcttcct gacggatgca caaggcgacc ggaagttctg gggtttcacc
     4501 tacgtcacct tgcggtttcc cctcgctctc aatgctgcgc ggctgccgct gctggttgaa
     4561 cggggctatg cctatgagtt gtggcgtacg gttccggaca cgggggagca ccagcgcata
     4621 gtgtcttccg gtgaggcgtc gttgatcgat cctgtgcagc gctccctgga gctgcccaat
     4681 ggtgtctgga cgctgagcgt ggccccgatc cagggctgga gtgaccagaa gcgctgggcc
     4741 atgaaggtcg ggtttggaat gttgttcagt gcattgatcg gttatctgac ctggctgctg
     4801 tatgccatgc ggctgcggga tgtgcatctg gaggcactgg tttcccggcg caccagcgag
     4861 atactgacca cccagcagca cctggaagca acggtcaatg cgattccgga cctgctgttc
     4921 gaggtggggc ttgatgggcg ctactacagt tcccattcgc cacgagcgga gttgctggca
     4981 cgtccagcag aggagttgca ggggcgcacg gtcaatgacg tcatgccccc tgaagcggcc
     5041 agggcggtac tggacgcttt gcaggaagcc aatgaaaaga cctggtctgt cggggtgcag
     5101 atttgtcttc cgctggacat cggatcccgc tggttcgaat tgtcggtagc cagaaaagcc
     5161 acggtgccgg gtactgagcc acgctttgtg gtcctctcgc gcgacattac caaaaccagg
     5221 gaggcggagg aaaagatccg acagctggcg catttcgatg cgttgacagg attgcccaac
     5281 cgcgtgctgc tggctgaccg gtgccactat gcgctgagta cggcacagcg caatgggcaa
     5341 tcggtggcat tgatgtttct cgatctggac catttcaaga atgtcaacga ttccctgggc
     5401 catcgcattg gcgatgcgtt gctgcgggcg ttggctggcc ggctgagtgc ctcggtgcgt
     5461 gaccaggata cggtgtcccg gctggggggt gacgaattta tcctggtatt gcccgacacg
     5521 gatgcgctag gtgctgcgca tgtggcagag aaattgatgg gtgccgcgct ggagcccttc
     5581 aatattgaaa cccacgaact gacagtcacg cccagcatcg gcattgcgat gtacccgaca
     5641 gacggtaccg atttcgacac cctgtcacgc tgcgccgatg cagccatgta ccacgccaaa
     5701 cagcgcggcc gtaaccatta ccgctttttt acagccgaaa tgcaggcaac gtctgagcgc
     5761 accctggtgc tggagaatgc cttgcgcagg gcgctggatc gcaaccagct ccagttgcat
     5821 taccagccgc aggtgtcgct ggataccggg gccatcgttg gcgcagaggc cttgttgcgc
     5881 tggaatcacc cggagcttgg ttccatctca cccgcagagt tcattccggt agcggaaagc
     5941 agtggactga tattgcccat cggtgaatgg gtgttgcgca ccgccgtgcg ccagctcagg
     6001 cagtggctgg gtgccggtgt ggcacctatc tgcatggcag tcaacctgtc ggcggtgcag
     6061 ttccgccagg ccgagtttcc gcaactcatt gcgcgcattc tcgaagagga aaatctgcct
     6121 gcgcaccttc tggagctgga actgacggaa ggcgtcgcca tgaccgatcc caatgctgcc
     6181 atcggcatga tggacaaact gcaccagcat ggcgtgcgaa tgtcgatcga cgatttcgga
     6241 ac
//
LOCUS       DAOSJM010000120         6179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11487_length_6179_cov_4.174069, whole genome shotgun sequence.
ACCESSION   DAOSJM010000120 DAOSJM010000000
VERSION     DAOSJM010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11487_length_6179_cov_4.174069"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PKX82_05710"
     CDS             complement(<1..231)
                     /locus_tag="PKX82_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51783.1"
                     /translation="MHSPLWPLLTSFSWQELRHHPWRNAAAVVSVMLGVALAFSVHLI
                     NASALDEFSQAVRSVGGQPDLELRFNPTAQTAD"
     regulatory      295..390
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            457..1488
                     /locus_tag="PKX82_05715"
     CDS             457..1488
                     /locus_tag="PKX82_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF51784.1"
                     /translation="MKHGIFSFLAAAIASLGLVNAASAADTAELRVLTHSSFAVPKPL
                     LAQFEKEAGVKLRITKAGDAGEMLNKLILTRAKPIADVVYGIDNALAPKALAAEVLDA
                     YNGPAATRAAVVPLPAPLVPVDYGYVTVNYDKAWFAKSGMALPKTLEELTQPAYAKLL
                     VVQNPATSSPGHAFLLATVGALGEEKAFDWWARMRSGGLKVAKGWSEAYYTEFSRNGG
                     KYPLVVSYATSPAAELFYAKDKPTEPPTGSLALAGGVFRQVEGVALVKGGGQRAAAQK
                     FVEFMRSVPVQQQMQTEMWMYPAEAGVARADVMKFAPEPAAFDTPAEKDLAARGADWV
                     ARWTRVVLK"
     gene            1488..3077
                     /locus_tag="PKX82_05720"
     CDS             1488..3077
                     /locus_tag="PKX82_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOF51785.1"
                     /translation="MRQGAAPLALAGLPLAFLLVVLVAPAVRLVAEGWPTDGHSLQAL
                     WAPWQDDYLRWRMVWSFLQALLTCAAALVLGLPLAWVLARLEFRGRSLVLRALMLPFV
                     VPTLVAALGVLALMGPHGVLVQLGGPDLQGTAWLLLYGNLFFNLCVVVRAGTDALEQV
                     SASRLAAARTLGASPWRAFWRVEWPAIAPWLMSALCLVFLYCFAGFGLALVLGGQRYA
                     TVEVEIYTLVAHELQLGQAGVLALWMLMLTALVAWGYAAIERQLATPGRADRQPRRRP
                     QGAGHWLLVVVALGVYMLICALPLVAIAWKAIISGAFLSSGEHAAWRVLLEEDTRMAL
                     WNTLRFSFSAVVLATVLGLAHALAARRSLALRTLVFLPFLVSPVMVAFGLLLLYPSWT
                     GNALLLVCAYALLAYPFVAKSVASALDSLPLYYLQAARSLGATPLRAFWRVTLPLLAP
                     ALRRGVAFAAATAVGEFAVSLFLSRPEWATLTTLIYQHLGRPGAANLDAALVLACLLM
                     ALTMGTFALIEWRGSREPDHA"
     gene            3070..3735
                     /locus_tag="PKX82_05725"
     CDS             3070..3735
                     /locus_tag="PKX82_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF51786.1"
                     /translation="MLELRSIHKTWEPRRGERRPLLNGVELRVASGQTVAILGASGCG
                     KSTLLKIGAGLEAPDSGTVWMDGQDITRMPPEQRHFALMFQDFALFPHLTVQDNVAFG
                     LVEQGIRKAQARSQAVEMLRRFGMEAFALSRVWQLSGGEQQRVALARALITRPRVLLL
                     DEPFSALDAELRVHLREEFRQHITAHGMATLWVTHDEAEARAVAQVGWRLEQGVLQAL
                     WGA"
     gene            complement(3751..4104)
                     /locus_tag="PKX82_05730"
     CDS             complement(3751..4104)
                     /locus_tag="PKX82_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51787.1"
                     /translation="MGWKISRFASSLVSLLRESGTDPAVAADIRLENARDAMLDLLQE
                     SLDGKVVRPAVWGKVLYARDIESLWYHRSDVMALLSVHLGENEARRRVTALTPLFKEM
                     QLGRKPGTPDRSDPT"
     gene            complement(4174..4824)
                     /locus_tag="PKX82_05735"
     CDS             complement(4174..4824)
                     /locus_tag="PKX82_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF51788.1"
                     /translation="MTLQVSGLHKHYGQTAVFSNVSLDVAPGEFVAIVGESGVGKSTL
                     LNCMAGLDSWDSGTVRLQGQDLGTLNDDARALLRRRAVGFVFQAFHVLPHLDVAQNVA
                     LPLLLLGQPDAARVQAMLDAVGLAGLEARLPQQLSGGQLQRVAIARALVHRPALLLAD
                     EPTGNLDPDTAAMVMEALVAQTRQHGASMVLVTHSVAATARADRVLRLRPDGVVPG"
     gene            complement(4821..5795)
                     /gene="miaA"
                     /locus_tag="PKX82_05740"
     CDS             complement(4821..5795)
                     /gene="miaA"
                     /locus_tag="PKX82_05740"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOF51789.1"
                     /translation="MAHPDSATPRCICLAGPTASGKTAAALAIAQELPVEIISVDSAL
                     VYRGMDIGTAKPSAQELASVPHHLINIRDPLQAYSAAEFVRDARALITDIRARGKTPL
                     LVGGTMLYFKALFDGLDDMPPADPAVRAVLEAEALAKGWPALHAELAAVDPVTAARLA
                     PADSQRIQRALEVYRISGQPLSHFHATRNATKSEAAHALSTGASDLISLEPVDRIWLH
                     GRIAQRFDAMLAAGFLDEVHALRARGDLHPDLPSMRCVGYRQAWEALDGKFPLTELRD
                     RGIFATRQLAKRQITWLRSMPQRHIVACDAPDALQQVLQRALQPVRVP"
     gene            complement(5782..>6179)
                     /locus_tag="PKX82_05745"
     CDS             complement(5782..>6179)
                     /locus_tag="PKX82_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bcr/CflA family drug resistance efflux
                     transporter"
                     /protein_id="HOF51790.1"
                     /translation="LCRRLLVRYGVRRTVAIAGGLSVTGGTAMGVMALAGVQSGWAIA
                     VPHAIFMLGHGIHQPCGQSGAVGPFPHAAGAASALNGFLMMVVAFAIGQWLGTHMDGT
                     VLPLTSGIWFWSTLIAASAWTLVQRHGTP"
BASE COUNT          969 a         1943 c         2155 g         1112 t
ORIGIN      
        1 gtccgcggtc tgcgcggtgg ggttgaagcg caattccaga tccggctgcc cacccaccga
       61 gcgcaccgcc tgcgagaact cgtccaacgc cgaggcgttg atcaggtgca ccgaaaacgc
      121 cagggccacc cccagcatga ccgacaccac ggccgccgcg ttgcgccacg ggtggtggcg
      181 cagttcctgc caggagaaac tggtgagaag gggccacagg ggactatgca tgggtagatt
      241 gtgcggggaa tggactgcgc cagcggcgat caacctgcga tcgacctaca atggcggctt
      301 caccgggggt gtccgcgcag gcggactgag aaatacccca tgaacctgat ctgggttatg
      361 ccagcgtagg aagcgtgaga agcccgtccc gtgttgcagt ggggttgtgg tttttgatgt
      421 gtccgatgcc ggcgcatttg aaaactggaa cgccatatga aacacgggat tttttctttt
      481 ctggccgcgg ccatcgccag cctgggactc gtcaacgctg ccagcgcggc agacacggcc
      541 gaactgcggg tgctgaccca cagctcgttt gcggtaccca agcctttgct ggcgcagttt
      601 gaaaaagagg caggcgtcaa attgcgcatc accaaggccg gcgatgccgg cgagatgctc
      661 aacaaactca tcctcacccg tgcgaaaccg atcgccgatg tggtctatgg cattgacaac
      721 gcgctggccc ccaaggcgct ggcggcagag gtgctggacg cttacaacgg cccggccgcc
      781 acacgcgctg cggtggtacc gctgcccgcg ccgctggtac cggtggacta cggctacgtc
      841 acggtcaact acgacaaggc ctggtttgcc aagagtggca tggccttgcc caagacactg
      901 gaagaactga cccaaccggc gtacgccaag ctgctggtgg tgcagaaccc ggcgacttcc
      961 agcccgggcc atgcctttct gctggccacc gtcggtgcgc tgggtgagga aaaagccttt
     1021 gactggtggg cgcgcatgcg cagcggcggc ctgaaagtcg ccaagggctg gagcgaggcg
     1081 tattacaccg agttctcgcg caatggcggc aaatacccgc tggtggtgag ctatgccacc
     1141 agtccggcgg ccgagttgtt ctacgccaag gacaagccga ccgagccccc aacaggcagt
     1201 ctggcactgg cgggtggcgt gttccgccag gtggagggcg tggcactggt caagggcggt
     1261 ggccagcgcg cagcggcgca gaagttcgta gagttcatgc gctcggtacc ggtgcagcag
     1321 cagatgcaga ccgagatgtg gatgtacccg gccgaggctg gcgtggcccg tgccgacgtg
     1381 atgaagtttg cgcccgagcc tgcggcgttt gatactcccg cagaaaaaga tctggccgcc
     1441 cgcggcgctg actgggtggc acgctggacc cgggtggtcc tcaagtaatg cgccagggcg
     1501 ccgcgccact ggctctggcc ggactcccgc tggcgttcct gctggtggtg ctggtggcgc
     1561 cggcggtgcg cctggtcgcc gagggctggc cgaccgacgg gcacagcctg caggccctgt
     1621 gggcgccgtg gcaggacgac tacctgcgct ggcgcatggt ctggtctttc ctgcaggcgc
     1681 tgctgacctg cgctgcagcg ttggtgctgg gcctgccact ggcctgggtg ctggcgcggc
     1741 tggagttccg gggccgcagc ctggtgctgc gtgccttgat gttgccgttt gtggtgccca
     1801 cgctggtggc ggcgctgggc gtgctggcac tgatggggcc gcatggcgta ctggtgcagc
     1861 tcggtggccc ggatctgcag ggcacggcct ggctgctgct gtatggcaac ctgttcttca
     1921 acctgtgcgt ggtggtgcgt gccggtaccg atgcgctgga gcaggtcagt gcctcgcgtc
     1981 tggctgctgc gcgcacgctg ggtgccagcc cctggcgtgc cttctggcgc gtggagtggc
     2041 cggccattgc gccgtggttg atgtcggcgc tgtgcctggt gtttctttac tgctttgccg
     2101 gtttcggtct ggcgctggtg ctgggcggtc agcgctatgc cacggtggag gtggagatct
     2161 acaccctggt cgcgcacgag ctgcaattgg gccaggccgg tgtgctggcg ctgtggatgc
     2221 tgatgctcac ggccctggtg gcatggggct acgctgccat cgagcgccag ctggccaccc
     2281 ccgggcgcgc cgaccggcag ccacgccggc ggccccaggg tgcgggccac tggctgctgg
     2341 tggtggtggc gctcggtgtt tatatgctga tttgtgctct acccctcgtc gccattgcgt
     2401 ggaaagctat catttcagga gcgtttctct ccagtggaga acacgcggcc tggcgcgtcc
     2461 tgctggaaga agatacccgg atggcgctgt ggaacaccct gcggttttct ttttccgcgg
     2521 tggtactggc taccgtgctg gggctggcgc acgcgctggc agcgcggcgt tcgctggcgc
     2581 tgcgcacgct ggtgttcctg ccgtttctgg tgtcgccggt gatggtggcg tttggcctgc
     2641 tgctgctcta tccgtcgtgg acgggcaacg ccttgctgct ggtgtgcgcg tatgccctgc
     2701 tggcgtatcc ctttgtggcc aagtcggtgg catcggcttt ggacagcctg ccgctgtact
     2761 acctgcaggc ggcccgctcc cttggcgcca ctcctttgcg cgctttctgg cgggtcacgc
     2821 tgccgctgct ggcgcccgcg ctgcgccggg gtgtggcctt tgcggcggca accgccgtgg
     2881 gggaatttgc cgtgagcctg ttcctctcac ggccggaatg ggccacgctc acgacattga
     2941 tctaccagca tctgggccgc cccggggctg ccaacctgga tgccgccctg gtgctggcct
     3001 gcctgctgat ggcgcttacc atgggcactt ttgcgctgat cgaatggcgc ggctcccgcg
     3061 aacctgacca tgcttgaact gcgttccatc cacaagacct gggagccccg gcggggcgaa
     3121 cgccgtccgt tgctcaacgg tgttgagctg cgcgtggcgt ccgggcagac ggtggcgatt
     3181 ctgggggctt ctggctgcgg caagagcacc ctgctcaaga tcggtgccgg tctggaagcg
     3241 cccgacagtg gcactgtgtg gatggacggg caggacatca cccgcatgcc gcccgagcag
     3301 cgccactttg ccctgatgtt ccaggacttt gcgctgtttc cgcacctgac cgtgcaggac
     3361 aacgtggcct tcggactggt ggagcagggc atccgcaaag cgcaggcacg cagccaggcg
     3421 gtggagatgc tgcgccgttt tggcatggag gcctttgccc tgtcccgggt gtggcaactc
     3481 tccggcggcg agcaacagcg cgtggcgctg gcccgggcgc tgatcacccg gccgcgggtg
     3541 ctgttgctcg atgagccgtt ctcggcgctg gacgccgagc tgcgggtgca cctgcgcgag
     3601 gaattccgcc agcacatcac agcccatggc atggccacgc tgtgggtgac gcacgacgaa
     3661 gcggaagcgc gggcggtggc ccaggtgggg tggcgtctgg agcagggggt gctgcaagcc
     3721 ctgtggggcg cctgacagcg caagcgaaag tcaggtcgga tcggagcggt cgggggtgcc
     3781 cggcttgcga cccagctgca tttccttgaa cagcggtgtc agtgcagtca cgcgccggcg
     3841 ggcttcgttc tcccccagat ggacggacag aagtgccatc acgtcactgc ggtggtacca
     3901 cagcgattcg atgtcccgtg catacaacac tttgccccat accgcgggac gcacgacctt
     3961 gccatccaat gattcctgca gcaggtccag catggcatcg cgcgcatttt ccaggcgaat
     4021 gtcggcagcg acggccggat ctgtgcccga ctcgcgcagg aggctgacca ggctactggc
     4081 aaagcgtgaa attttccaac ccatgtggtg tcgttatgtt cttgatggac tgacagtgga
     4141 ccacgaaccg gcgtggcatg ctgtaacagt ttgttagccc gggaccactc catcgggtcg
     4201 caggcgcagc acacggtctg cgcgggccgt agcggccacc gagtgcgtca ccagcaccat
     4261 ggacgcgcca tgctggcgcg tttgcgccac cagtgcttcc atcaccatgg ccgcggtgtc
     4321 ggggtcgagg ttgcctgtgg gttcatcggc cagcagcagg gccgggcggt gcaccagtgc
     4381 gcgtgcaatg gccacacgct gcagctggcc accactgagc tgttgtggca ggcgagcctc
     4441 cagcccggcc agaccgacag catccagcat ggcctgcacc cgcgcggcat ccggttggcc
     4501 cagcagcaac aatggcaggg ccacgttctg cgctacatcc agatgcggca gcacatgaaa
     4561 ggcctggaac acaaagccca cggcccgccg acgcagcagt gcccgtgcat cgtcgttgag
     4621 ggtgccgagg tcctgcccct gcagacgcac ggtgccgctg tcccagctgt ccaggcctgc
     4681 catgcagttg agcagcgtgg atttgcccac accggactcg cccacaatgg ccacaaactc
     4741 gcccggtgcg acgtccagcg acacgttgga aaacaccgcg gtctggccgt agtgcttgtg
     4801 gagcccggaa acctgcaggg tcatggcaca cgcaccggtt gcagcgcccg ctgaagtacc
     4861 tgttgcagcg catcgggtgc gtcgcaggcc acgatgtggc gctggggcat cgagcgcagc
     4921 caggtgatct gccgtttggc caactgccgt gtggcaaaga tgccgcggtc gcgcagttcg
     4981 gtcaggggga acttgccatc cagcgcctcc catgcctggc ggtaacccac gcagcgcatc
     5041 gagggcaggt cggggtgcag gtcgccacgc gcacgcaggg catgcacctc gtcgagaaag
     5101 cccgctgcca gcatggcatc aaagcgctgg gcaatgcggc catgcagcca gatgcgatcc
     5161 accggttcaa gagaaataag gtcgctagcc cccgtggata gtgcgtgagc agcttcactt
     5221 tttgtagcat tgcgggtcgc atggaaatgc gacagtggct ggcccgagat ccggtacacc
     5281 tccagcgcgc gctggatgcg ctggctgtcg gcgggggcca ggcgtgcagc agtcaccggg
     5341 tccaccgcag ccagttcggc atgcagggcg ggccagcctt tggccagtgc ctcggcttcc
     5401 agtacggcgc gcaccgcggg gtcggccggt ggcatgtcgt ccaggccgtc gaacagcgcc
     5461 ttgaaataca gcatggtgcc gcccaccagc agcggcgtct tgccgcgggc gcggatgtcg
     5521 gtgatcaggg ccctggcgtc gcgcacaaac tcggccgcac tgtaggcctg cagcgggtcg
     5581 cggatgttga tcaggtggtg gggcacactg gcgagttctt gcgccgaggg cttggcggtg
     5641 ccgatgtcca tgccgcggta caccagcgcc gaatccacgc tgatgatttc gaccggcagt
     5701 tcctgcgcga tggcgagcgc ggcggccgtc ttgcccgacg ccgtggggcc ggccaggcag
     5761 atgcagcggg gcgtggcgga gtcagggtgt gccatggcgt tgtaccagcg tccatgcgct
     5821 tgcggcaatc agcgtgctcc agaaccagat gccgctggtc agcggcagca cggtgccgtc
     5881 catgtgggtg cccagccact gcccgatggc aaaggcgacg accatcatca gaaagccgtt
     5941 gagtgccgag gccgctccgg ctgcatgcgg gaaggggccc acggcgccgc tttgtccgca
     6001 aggttggtgg atgccgtggc ccagcatgaa gatggcatgg ggcaccgcaa tagcccaacc
     6061 cgactgcacg cccgccagcg ccatgacgcc catggccgtg ccaccggtga ccgacaggcc
     6121 gcccgcaatg gcgactgtcc tgcgcacgcc gtagcgcacc aacagacgcc ggcacagga
//
LOCUS       DAOSJM010000121         6127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11605_length_6127_cov_3.530962, whole genome shotgun sequence.
ACCESSION   DAOSJM010000121 DAOSJM010000000
VERSION     DAOSJM010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11605_length_6127_cov_3.530962"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /gene="rlmD"
                     /locus_tag="PKX82_05750"
     CDS             complement(<1..1066)
                     /gene="rlmD"
                     /locus_tag="PKX82_05750"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOF51791.1"
                     /translation="MTEETHKNDALPPGALAVESLDLEAQGVAHRPDGKVVFIDGALP
                     FEVVSAQIHRSKNSFEKGTLSVIHRESSQRVRPQCPHFGLHEGACGGCKMQHLHAGAQ
                     VAVKQRVLEDNLWHIGKVKPDNILRPIEGPAWGYRYRARLSVRHVRKKGAVLIGFHER
                     KSRYVADMKECHVLAPQVSALLLPLRALIASMDAVETLPQIELAVGDMGGSTGTGDVI
                     ALVLRHMEPLSSADLQRLQQFAVAHRGVQWWLQPKGPETVQRMDELPGHATAQLAYQL
                     PDFGITMPFRPTDFTQVNPHINRVLVTRALGLLAVQQQERVIDWFCGLGNFTLPLATQ
                     AREVLGVEGAESLVARSRENY"
     gene            1112..1606
                     /locus_tag="PKX82_05755"
                     /pseudo
     CDS             1112..1606
                     /locus_tag="PKX82_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            1624..4128
                     /locus_tag="PKX82_05760"
     CDS             1624..4128
                     /locus_tag="PKX82_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF51792.1"
                     /translation="MIQQKHQHPYQMAFGLALLGLAAPLLWRILQGASPLDLPVAQLE
                     FFRAVADLLAVVVSMLICITGYRAIMSHRKAATVWLGVAFLGVGLFEVLHLLSYSSMP
                     DVITPNTPVKPAFFWLAARLLAALALLVYALLPLHSEVSLPRKRFALGIMLFVVGAVA
                     YAGLIWPESFPALVDSAGKATTLKNGLESLVLGLHLTTLVVFWFRRKVLAGECLMALV
                     FAVALSCVSEIFVLLPVDRDANSAVVLGHAYKVLAYLYLFHATFHEAMRRPLEHIHRQ
                     HLRENVTLSAAPDGVLWVDDSGRILTANPAMETLSGYRPEQLLGKNVDIFLPQHLRAS
                     HGNSVRDFFVNPHPRAMGNMDLKLMRKDGSMLPVDISLGKWIENGQNHAIAYIRDLTE
                     RKKFEESLRHKATHDELTGLPNRWMYQLQLNQALAHAGRNRQRVAVLLLDLDYFKTIN
                     DSFGHAAGDALLVQAGNRMRSTLRESDLLARLGGDEFAVLLTELADADEAVSVATKVL
                     AALQEAYVLQGHDVYSGGSVGLSFFPDDAQDGDTLLRYADLAMYQAKQAGRGTYACYS
                     QNMDVRVHEDMLLHTRLKEALATGALELHYQPQVDVESGNVTGAEALLRWQDPVLGDV
                     TPSRFIPVAEATGLILPLGDWVLETACRQIATWTRIGLPVRVAVNFSAQQFRRFDLAD
                     KVRAVLEHTGAQAQWLDIEITESMAMTQPEQARKQLEALVALGCSVALDDFGTGYSSL
                     AYLKTLPVSKLKIDKSFMDGIPHDGNDVAISRAIIALAHSLGLTLVAEGVETDAQLAF
                     LRQYGCETYQGWLFAKAMPAEAMTQRMSDWAPLSGF"
     gene            complement(4144..5169)
                     /locus_tag="PKX82_05765"
     CDS             complement(4144..5169)
                     /locus_tag="PKX82_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOF51793.1"
                     /translation="MAKSRKTGDQGLVAAPAVVREEFEENTALAPVEHAQDAIEIVAP
                     AVLDVLARDARAGDESADSLALYLRGVRRTELFTPEEEFACATRARAGDFAARQSMIE
                     HNLRLVISIAKGYMGRGVPMADLIEEGNLGLMHAIDKFEPERGFRFSTYATWWIRQSV
                     DKALMYQGRAVRLPVNVVREVQQVLRARRMLENDAVLVAQRPEGIRAEDIGALLGRPA
                     TEVAELLVMAEAPRSLDAAIDSADDERTLGDSLVDSLALDPEDVTQGHEVDHLLDTWV
                     ATLSGREREVLEGRFGLHGREPETLEVLSVRLGLTRERVRQLQNEALFKLKRYISRRG
                     IHRDALL"
     gene            complement(5182..6048)
                     /locus_tag="PKX82_05770"
     CDS             complement(5182..6048)
                     /locus_tag="PKX82_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOF51794.1"
                     /translation="MSGLLRRGSCLLAVGGALLLVGCGSKSLNRAPVEDRGNAVGAPA
                     SPDTRPQVARQLPGFENAGKPGYYTVKPGDTLIRIGLETGQSHKDISRWNALENPNRI
                     EVGQVIRVAPPATETAAVVARPISSGTVVSTAIPVGAPAKAASAPASAPVAPAPVAAA
                     SSATGEDNLGWIWPGNGQVLAGFDEVKNKGVDIGGALGDPVLAAAEGKVVYAGSGLRG
                     YGNLIILKHNNTYLTAYAHNKSLLVKEDQTVKKGQKIAEMGDSDADRVKLHFEVRRQG
                     KPVDPSKYLPAR"
BASE COUNT         1120 a         1984 c         1865 g         1158 t
ORIGIN      
        1 cataattctc gcgggaacgg gccaccagcg actcggctcc ctctacaccc agcacctcgc
       61 gcgcctgcgt ggccagcggc agggtgaagt tacccagacc acagaaccag tcaatcaccc
      121 gctcctgctg ttgcaccgcc agcaggccca gcgcgcgggt caccagcacc cggttgatgt
      181 gcgggttgac ctgggtgaaa tcggtcgggc gaaatggcat ggtgatgccg aagtctggca
      241 actggtaggc cagttgcgcc gttgcatggc caggcagttc gtccatccgc tgcacggtct
      301 ctgggccctt gggctgcagc caccattgca caccgcggtg cgccacagcg aactgctgca
      361 gacgctgcaa atcggcgctg ctgagcggtt ccatatgccg cagcaccagc gcgatgacgt
      421 cgcccgtgcc ggtactgccg cccatgtccc ccacggccag ttcaatctgc ggcagtgtct
      481 ccaccgcgtc catggaggca atcagggcac gcaagggcag cagcagcgcg ctgacctgcg
      541 gcgccagcac atggcactct ttcatgtccg ccacatagcg gcttttgcgt tcgtgaaagc
      601 cgatgagcac cgcacccttc ttgcgcacgt ggcgcaccga caggcgcgca cggtagcggt
      661 agccccaggc cgggccttcg atggggcgca ggatgttgtc gggcttcacc ttgccgatgt
      721 gccacaggtt gtcttccagc acccgctgct tgacggccac ctgcgcgccc gcgtgcagat
      781 gctgcatctt gcaaccaccg cacgcgcctt cgtgcaggcc gaaatgcggg cactggggcc
      841 gcacccgttg cgacgattcc cggtggatga cgctcagggt gcctttctcg aaactgttct
      901 tgctgcggtg gatctgcgcg ctcaccacct caaaaggcag ggcgccgtca ataaagacca
      961 ccttgccatc cgggcggtgg gcaacgccct gggcttccag gtcgagggat tcaacggcca
     1021 gtgcgcccgg tggcagcgca tcatttttgt gggtttcttc tgtcatgccg ctattgtccc
     1081 cgaggttaga ctcggcaagg cactcacgct gatttcggat tccagtcgca ggattgccga
     1141 catcatcagt gtgatcgatg gcatcgcctt ccagaccaat atcctggccc tgaatgccgc
     1201 agtagaagcc gcacgggccg gggagcaagg tcgcggcttt gccgtggtag ccagcgaagt
     1261 gcgtagcctg gcgggcagat cggcagcggc agcccgggaa atcaaggcct tgatcgagga
     1321 ctccgtcaac aaggtggaga acggctcccg tctggttgcc gacgcagggc agaccattgc
     1381 agaggtggtc gcccaggtcc ggcatgtcaa cgatttgatc gcagaaatca cggcagcgtc
     1441 tgcagagcag accgccggta tcacccaggt aggacaggcc gtgaaccagc tcgacacggt
     1501 cacccagaaa aatgccgcaa tggtcgagga aagcatggcc tcagccatcc ggatgggcga
     1561 acaggcccag cgcctggtgg atgccgtcaa ggtgttcgcg gcataacacg gcatggcaac
     1621 cccttgattc agcagaaaca tcagcacccc taccagatgg cgttcggcct ggctttgctg
     1681 ggcctggctg cgcccctgct ctggcgtatc ttgcagggag ccagtcccct tgatctgcca
     1741 gtagcgcagc ttgaattttt ccgggccgtc gcagatctct tggctgtggt ggtttccatg
     1801 ctgatttgca ttaccgggta ccgcgccatc atgtcgcacc gcaaggcggc cacggtctgg
     1861 ctcggggtgg cattccttgg agtgggtttg ttcgaagtcc tgcacctgct gagctacagc
     1921 agcatgcccg acgtcatcac acccaatacc ccggtgaagc cggcattttt ctggctggcc
     1981 gcccgcctgc tggcggccct ggcgttgctg gtctatgcac tgctccctct gcactccgaa
     2041 gtcagcctgc cacgcaagcg ctttgctctg ggaatcatgc tgttcgtggt cggcgcagtg
     2101 gcgtatgcgg gtctgatctg gccagaaagt ttcccggccc tggtggattc cgccgggaaa
     2161 gccactacgc taaaaaacgg gctggaatcc ctggtgctgg gcctgcacct caccacactg
     2221 gtcgtattct ggttcaggcg caaggtactg gcaggtgaat gcctgatggc gcttgttttt
     2281 gcggtggcgc tctcgtgcgt ttcggaaatt tttgtcctcc tgccggtgga cagagacgcc
     2341 aatagtgcag tggtgctggg ccatgcctac aaggtgttgg cctacctcta cctgttccat
     2401 gccacattcc atgaggccat gcgccgcccg ctggagcata tccaccgaca gcacctgcgc
     2461 gagaacgtga cactcagtgc cgcaccggac ggggtcctgt gggtggacga cagcggtcgc
     2521 attctcacgg ccaatccggc gatggaaacg ctgtccggct accgtccgga acagttgctg
     2581 ggcaagaatg tggacatatt cctgccgcag cacctgcgcg cgagccacgg caattcggtc
     2641 cgagacttct tcgtcaatcc ccatccgcgc gccatgggca atatggacct gaagctgatg
     2701 cgcaaagacg gcagcatgct gccggtagat atctctctgg gcaagtggat agaaaatggc
     2761 cagaaccacg ccattgccta tatccgcgat ctgactgaac gcaagaagtt tgaagagtcg
     2821 ctgcgacaca aggccacaca cgacgagttg accggcctgc ccaaccgctg gatgtaccag
     2881 cttcagctca accaggcact cgcccacgcc gggcgcaatc gccaaagggt tgcggttctg
     2941 ttgctagacc tggactactt caagaccatc aacgacagct tcgggcacgc cgcgggagac
     3001 gccctgctgg tgcaggcagg caaccgcatg cgcagcacac tgcgcgagag cgacctgctg
     3061 gcacgcctcg gaggtgacga atttgcggtt ctgctcaccg aacttgccga tgccgacgaa
     3121 gccgtcagtg tggcaaccaa ggtactcgca gccctgcagg aggcgtatgt gttgcaggga
     3181 cacgatgtgt attcgggcgg cagcgtcgga ctatccttct tcccggacga tgcacaggat
     3241 ggcgacacct tgctgcggta cgccgacctg gcgatgtacc aggccaaaca ggccggccgg
     3301 ggtacctatg cctgctactc ccagaatatg gatgtgcgtg tccacgaaga catgcttctg
     3361 cacacccgtc tgaaggaagc ccttgccacg ggcgcgctgg agctgcacta ccagccccag
     3421 gtggatgtgg aaagcggcaa cgtcaccggc gccgaagccc tgctgcgctg gcaagacccc
     3481 gtgcttggtg atgtcacccc ctcacggttc atccccgtgg cggaggctac aggcctgatt
     3541 cttccgctgg gcgactgggt gctggaaacg gcctgtcgtc aaatcgcaac ctggacacgt
     3601 atcggcctgc cagtgcgcgt ggcggtcaac ttctcggccc aacaattccg gcgctttgat
     3661 ctcgccgaca aggtccgtgc ggtactcgaa cacaccggag cgcaggcgca atggctggac
     3721 atcgagatca ccgagtccat ggccatgaca caacccgagc aggcacgcaa gcagctcgaa
     3781 gcgctggtgg cactgggatg cagcgtggcg ctggacgact ttggtacagg ctattcgtcc
     3841 ctggcctacc tcaagacact gcccgtgagc aaactcaaga tcgacaagag tttcatggac
     3901 ggtattccgc acgacggcaa cgacgttgcc atctcgcgcg ccatcattgc gctggcccat
     3961 agcctaggcc tgacgctggt cgccgagggc gtggaaaccg acgcgcaatt ggcctttctg
     4021 cgccaatacg gctgcgaaac ctaccagggc tggctgtttg ccaaggccat gccggccgag
     4081 gccatgacac agcgcatgag cgactgggct ccactctcgg gcttctgaag tcctctgacg
     4141 cgtctacaac aaagcgtcgc ggtggatgcc gcggcggctg atgtagcgct tgagtttgaa
     4201 cagggcttcg ttctgcagct ggcgcacccg ttcgcgggtc aggcccagcc ggacgctcag
     4261 cacctccagt gtctcgggct cgcggccatg caggccaaag cgtccctcaa gcacctcgcg
     4321 ttcccgcccc gacagcgtgg ccacccaggt gtcgagcagg tggtccacct catgcccctg
     4381 cgtgacatct tcggggtcga gtgccaggct gtccaccaga ctgtcgccta gcgtgcgttc
     4441 gtcatcggca gagtcgatgg cggcgtcgag ggagcgtggc gcttcggcca tgaccagcag
     4501 ttccgccact tccgtggcgg gcctgcccag cagcgctccg atgtcttccg cgcggattcc
     4561 ctccgggcgt tgcgccacca gcacggcatc gttctccagc atgcgccgcg cgcgcagcac
     4621 ctgctgcact tcccgcacca cattgaccgg cagacgcacc gcccggccct ggtacatcag
     4681 cgccttgtcg acggactgac gaatccacca ggtcgcgtag gtggaaaagc ggaaaccgcg
     4741 ctcgggctca aacttgtcga tggcgtgcat cagccccagg ttgccttcct cgatcaggtc
     4801 cgccatgggg acgccgcgcc ccatgtagcc tttggcaatg ctgatcacca gacgcaggtt
     4861 gtgctcgatc atgctctggc gggctgcaaa gtcgcctgcc ctggcgcgtg tggcgcaggc
     4921 gaattcttct tccggggtga acagttcggt gcggcgcacg ccgcgcaggt acagggccaa
     4981 cgagtcggca gattcgtcgc ccgccctggc gtcacgggcc agtacatcca gcactgcagg
     5041 cgctactatt tcaatagcat cctgcgcatg ttctacgggg gctagagccg tattttcctc
     5101 aaattcctcc cgcaccacgg ctggagcggc caccaggccc tggtctccag ttttgcggga
     5161 tttggccatg gtgtcaggcc atcacctggc gggcaggtac ttggaggggt cgaccggctt
     5221 gccctggcgc cgcacctcga agtgcagctt gacgcgatcc gcatcgctgt cgcccatctc
     5281 ggcaatcttc tgccccttct tgacggtctg gtcttccttg accagcaggg acttgttgtg
     5341 ggcgtacgcg gtcaggtagg tgttgttgtg cttgaggatg atcaggttgc catagccacg
     5401 cagaccggac cctgcgtaca ccaccttgcc ctcggcggcc gccagcaccg ggtcacccaa
     5461 ggcgccaccg atgtctacac ccttgttctt cacctcgtca aaacccgcca gcacctggcc
     5521 attgcctggc catatccagc ccagattgtc ttcaccggtc gcagaactgg cggcagcgac
     5581 gggcgccggg gccacaggtg cgctggccgg ggccgatgcc gccttggctg gagcgcccac
     5641 cggaattgcc gtggaaacca cggtaccgct ggagatcggt cgggcgacta ccgccgctgt
     5701 ttcagtggcg ggaggagcga cacggatcac ctgtcccact tcaatgcggt tcggattctc
     5761 cagggcattc cagcgcgaaa tatccttgtg gctctggcca gtctccaggc cgatgcggat
     5821 cagggtgtca cccggcttga cggtgtagta cccgggcttg cccgcattct cgaacccggg
     5881 caactgccgt gcgacctggg gacgtgtatc cgggctggca ggcgcgccaa cggcgttacc
     5941 ccggtcttcc accggcgcac gattgagcga cttggatcca catcccacca acaggagtgc
     6001 tccaccaact gccaacaaac aggaaccacg acgcaacaaa ccagacataa acactttcct
     6061 tcaggcaacg cccgatttta gagggacaaa gtggacaggc tccagcaggg tctgccgcac
     6121 gccctgg
//
LOCUS       DAOSJM010000122         6126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11606_length_6126_cov_4.600725, whole genome shotgun sequence.
ACCESSION   DAOSJM010000122 DAOSJM010000000
VERSION     DAOSJM010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11606_length_6126_cov_4.600725"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="PKX82_05775"
     CDS             <1..942
                     /locus_tag="PKX82_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOF51795.1"
                     /translation="MNLNDIKNPHLIYPGQILILERKNGMATLRVANPVSADSGMETV
                     RVSPRTRYESLSGNALTALQSSVIEPFLAEPLVVDEATLNAAPRIVSAQESRVLLAKG
                     DRAYARGSNGAPLLDDQPREKQFRVFRNATPLKDPGTGEILGYEAQYVGKASLVRSES
                     TTEVTDKDGTTTGVIVPATIDIVGAKEEMRVGDRMLPEPPRQLQTYTPRAPSAAVTGR
                     IVSVYGSAVVNAAQNQVVAINLGSRDGMEPGHVLAILKDGARVIDKGDAALPLMKLPD
                     ERNGLLMVFRTFDRVSYALILDITNGVRVGDRLTAPR"
     gene            966..2099
                     /gene="dprA"
                     /locus_tag="PKX82_05780"
     CDS             966..2099
                     /gene="dprA"
                     /locus_tag="PKX82_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOF51796.1"
                     /translation="MDREELRAWLRLQLTPGVGDVTARKLLTQFGLPDNVFKQSADAL
                     RQVTSALQATALLEAPPELEALVEVTWNWLQAEPGCRYVLPLGDPGYPQTLLDIEDPP
                     LLLYGMGPADIWQRGGLNMVSNRCLAVVGSRNPTPQGTANARQFAVALTQAGLTIVSG
                     MALGVDGAAHEGALEGAALDQLATVAVVGTGLDRVYPKQHRVLAHRIAQRGLLLSEYP
                     LGTPPLAANFPKRNRIISGLSRGTLVVEAALQSGSLITARLATEQGKDVFAIPGSIHS
                     TQARGCHALIKQGAKLIECAQDVLEELQMDGGTTPPTNAQTPAPEESALLHALGYDAT
                     SLDTLQARTGLDTATLQAQLMTLELQGQIARLPGGLFQRLVVV"
     gene            2166..2633
                     /locus_tag="PKX82_05785"
     CDS             2166..2633
                     /locus_tag="PKX82_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HOF51797.1"
                     /translation="MFEVLAFVYENYYTGETRPEPAHLKRKLSAVGFESDEIGDALAW
                     LNGLDTAACTTQPEPWLVQPSPSSIRIYPAHEQNHLGRQALGFIRFLESANVLPPHMR
                     EVVIDRAMAAPGGPIGLDDLKIIVLMVYWGFGQEPDALVLDELCDDAQVRIAH"
     gene            complement(2655..5228)
                     /locus_tag="PKX82_05790"
     CDS             complement(2655..5228)
                     /locus_tag="PKX82_05790"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOF51798.1"
                     /translation="MATPSTLSPRLQLARAVRERFVAEMGRAMSELSVQVQVRLTELV
                     DEPCNARESQVRRDVWMAYKRVRPLWLENTLKAWRTCLEPTKDAKPKNSLEAAGLELV
                     GTEVVENKILASRLVMTVNDKVFAQLDDLRVRIKLLENLDDLDGGDILRPEVLVLQMV
                     EQWAQSGMPGDSWPLVNDTAQAKLITSLNEAYKNGNTLMISKGVLPTIELKDRVRAPV
                     RAAGPRPRPSAPPPGTPQQAQDPHSSGLGDLGYGGGYPQDRNQGGSAQAHRSGQQGYS
                     GQPQEAAQRSGGFFGGRLGWGNSAPAENASGGSAGHNSTRDAGGPQSAQPSGSTPFWK
                     QSGAGQSGPGQHYSAGDETRMMTTNTPLARARSRAQGVIGQIKRLFVNHAGGDFAATA
                     HYTPSPALAQAIAPQAVAASYATGGTMYEDYSPAGVARVAGALRDQTVELKKKAETKG
                     EKATIEIVALMFQAILAEERIPPGIRVWFARLQMPVLRVALEDPDFFGTTDHPARLLI
                     DRMGSCVMGFDATGVQGGAMEIEVKRIVQVVEQYPETGKKVYQIVYDEFQKFLSKYLT
                     EKSTTQKVVSVAQQVEQKETLAIQFTIEMRNMLKDLPVRDEIRDFLFKVWAEVLAVSA
                     LRKGPKHADTQTLKKSATDLVWAASAKPNRADRARVIQDLPNLLVRLRSGMTLLGMPP
                     SEQEANVKKISDTLADAFLSKTQAIPQAKIDAMAQRLGNLEDFVSEDGMGDLPLDAES
                     IEMMLGIEASEIEVVANGGSKPTAAMLAWAQELQVGSWFTLDHNNQISQVQFAWRSER
                     KHLNLFASTSGKSFLIQGGRLAAYLQAGLLLPQEEEALTVRATRDAMAKLEANPERLL
                     A"
     gene            complement(5240..>6126)
                     /gene="secF"
                     /locus_tag="PKX82_05795"
     CDS             complement(5240..>6126)
                     /gene="secF"
                     /locus_tag="PKX82_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOF51799.1"
                     /translation="LATFLAAVFFLFSRGLHLSVEFTGGTLMEVSYSQPADLNSIRAT
                     VAKLGMTDVQVQNFGTAQDVLIRMPVQKGSSSGQQSEQVLAALKADNATASLRRTEFV
                     GPQVGDELAADGLKALAFVVVGIMLYLAIRFEWKFAVAAIIANLHDVIIILGFFAFFQ
                     WEFSLPVLAAVLAVLGYSVNESVVIFDRIRENFRRYRKMNTVEIIDNAITSTISRTII
                     THGSTQLMVLSMLLFGGATLHYFALALTIGILFGIYSSVFVAAAIAMWLGIAREDLIK
                     ATAKKEEDPNDPNAGATV"
BASE COUNT         1273 a         1891 c         1718 g         1244 t
ORIGIN      
        1 atgaacctca acgacatcaa gaacccacac ctgatttacc ccggtcagat actgatcctg
       61 gagcgcaaga acggcatggc taccttgcgc gtggccaatc cggtgtctgc cgacagcggc
      121 atggaaacgg tgcgtgtatc cccgcgcacc cggtacgaat ctctgtccgg caatgcactg
      181 actgcactcc agtccagcgt tattgagccc ttcctggccg agccactggt ggttgacgaa
      241 gcaactctta acgctgcgcc gcgcattgtc tctgcgcagg aaagccgtgt cctgcttgcc
      301 aaaggcgatc gcgcctatgc gcgtggctct aacggcgctc cccttctgga tgaccagccg
      361 cgcgaaaagc aattccgggt attccgcaat gcaacgcccc tgaaagaccc tggaacggga
      421 gaaatcctgg gctatgaggc gcagtatgtc ggcaaggctt cgctggttcg cagcgagagc
      481 accacagaag tgaccgacaa ggacggaacc accactgggg tcattgttcc cgccaccatc
      541 gacatcgtcg gggccaagga agaaatgcgt gtgggtgacc gcatgctgcc tgagccaccg
      601 cgccagctcc agacctacac accgcgggcg ccctctgctg ccgtgacggg gcgcatcgtc
      661 tctgtatatg gcagcgctgt ggtaaacgct gcccaaaacc aggtggttgc catcaatctc
      721 ggttcccgtg acggaatgga gcctggccac gtgctggcca tcctgaaaga cggcgcccgt
      781 gtgattgaca agggggatgc tgcactccca ctgatgaagc tgcctgacga acgcaatggc
      841 ttgctgatgg tgtttcgcac cttcgatcgc gtttcctatg cgctgattct ggacatcacc
      901 aatggcgtgc gtgtgggtga ccgcctgact gccccgcgtt aacgacggcg ccatccaagg
      961 cttccatgga tcgcgaggag cttcgtgcct ggttacggct acagctgaca ccaggcgtcg
     1021 gtgatgtaac cgcccgaaag ctgctgacgc aattcgggct cccggataac gtcttcaagc
     1081 aatctgccga tgcactcaga caggttacca gtgcattgca agcaacagca ctgttggaag
     1141 ctccccctga actggaagcg cttgtagaag tcacctggaa ctggcttcag gcagaacccg
     1201 ggtgcaggta cgttctgcca ctgggtgacc caggttatcc gcagaccctg ctcgatattg
     1261 aagatccccc gctcctgctt tacggcatgg gccctgcaga catctggcag cgtggtgggt
     1321 tgaacatggt ttccaaccgg tgcttggccg tcgttggcag ccgtaacccc acaccacaag
     1381 gcactgccaa cgcacggcag tttgccgtcg ccttgacgca ggccggtttg acgatcgtca
     1441 gcggcatggc actgggagta gacggcgcgg cgcacgaagg cgcactggaa ggcgccgcac
     1501 tggaccagct ggccacggtt gccgtcgtcg gaacaggact tgatcgggtg tatcccaagc
     1561 aacaccgcgt tttagcgcac cgcatcgccc agcgcggcct gttgctcagc gagtaccctc
     1621 tcggcacccc gccactggca gccaatttcc cgaagcgcaa tcgcatcatt tccggtttgt
     1681 cccgcgggac attggtggtg gaggctgcgc tgcaatcggg ctcgctgatc acggcacggc
     1741 ttgccaccga acagggcaag gacgtgtttg ccattccagg atcgattcat tcaacccagg
     1801 cccgcggttg ccacgcactg atcaagcagg gtgcgaaact gatcgagtgc gcgcaggatg
     1861 tgctcgaaga attgcaaatg gatggcggga ctaccccccc aaccaatgcg cagacacccg
     1921 cacccgagga gtccgcactc ctgcatgcgc tgggatatga cgcaaccagt ctggatacgc
     1981 tacaggcgcg cactggcctg gataccgcca cgctgcaggc ccagttgatg acgcttgaat
     2041 tgcagggaca gattgcacgg ctgccaggcg ggctttttca gcggctggtc gtggtctaac
     2101 gcgagaaaag cagggcaagt ccggatgcgt ccgggaagtc gtttgatctg cgatatattg
     2161 cgttcatgtt tgaagtgctc gctttcgttt acgaaaacta ctacaccggg gaaacccgcc
     2221 ccgaacccgc tcacctgaag cgaaaactca gcgccgtggg gtttgaatcc gatgaaattg
     2281 gcgatgcctt ggcatggctc aacggacttg acacagccgc ctgcacgacc cagccagaac
     2341 catggctcgt tcagccaagc ccttccagca tacggatata tccagcacat gagcaaaacc
     2401 atcttggccg ccaggccttg ggatttatcc gctttcttga gtctgccaac gttctgccac
     2461 cccatatgcg ggaagtcgtt attgaccgcg cgatggcagc accaggtggc cccatcgggc
     2521 tggatgacct gaagatcatc gttctgatgg tgtattgggg ctttgggcag gaacccgacg
     2581 cattggttct ggacgaattg tgtgacgatg cccaggtgcg catagcgcat tgacagcgcg
     2641 atctcgcgca gaaatcaagc cagcaggcgc tcgggattgg cttccagttt ggccatggca
     2701 tcgcgcgtgg cccgtactgt caaagcttct tcttcctgcg gcaacaacaa ccctgcctgc
     2761 aggtaggccg ccaggcgacc tccctgaatc aggaagctct ttccagaagt ggatgcaaac
     2821 aggttcaaat gcttgcgctc actgcgccat gcaaactgca cttgggatat ctggttgttg
     2881 tggtccagcg taaaccacga gcccacctgc aactcctgcg cccaggccag catggccgcg
     2941 gtgggcttgg agccgccatt ggcaaccacc tcgatctcgg acgcttcgat acccagcatc
     3001 atctcgatgc tttcggcatc cagaggcaaa tcacccatgc catcttccga cacaaagtcc
     3061 tccagatttc ccagacgctg cgccatggca tcaatcttgg cttgcggtat ggcctgtgtc
     3121 ttggacaaga acgcatccgc cagcgtatcg ctgattttct tgacgttggc ctcctgttcg
     3181 gagggcggca tcccaagcag agtcatgcct gagcgcagcc gcaccaaaag gttcggcaga
     3241 tcctgaatga cccgggcacg gtctgcgcga ttgggttttg cactggccgc ccacaccaga
     3301 tccgtggcag atttcttcag ggtctgcgta tccgcgtgtt tgggtccctt tcgcagggca
     3361 gacacggcca atacctcggc ccataccttg aacaggaagt cacgaatctc atcgcgtacc
     3421 ggcaagtcct tgagcatgtt gcgcatttcg atcgtgaact ggatcgccag cgtctctttc
     3481 tgctcaacct gctgggccac actgaccacc ttctgggtgg tgcttttttc ggtcaggtat
     3541 ttcgagagga acttctggaa ttcgtcatag acgatctggt agaccttctt gccggtttcc
     3601 gggtattgct cgactacctg aacaatgcgc ttgacctcga tctccatggc gccaccctgc
     3661 acacccgttg catcaaagcc catgacacaa gagcccatgc ggtcaatcag caaacgggca
     3721 gggtggtctg tcgtgccaaa aaaatcggga tcttccagtg ccacacgcag gaccggcatc
     3781 tgcaagcgtg cgaaccagac acggattccg ggcggaatac gttcctcagc cagaatggcc
     3841 tgaaacatca gggcaacaat ttcgatggtt gccttctctc ccttggtttc ggcctttttc
     3901 ttgagctcaa ccgtctgatc acgcagggcg ccagcaaccc gggcaacacc ggccgggctg
     3961 tagtcctcgt acatcgtgcc gcccgtcgca taggaagcgg caactgcctg gggtgcaatg
     4021 gcctgtgcca gcgctgggga cggcgtgtaa tgcgccgtcg cggcaaaatc accgcctgca
     4081 tggttgacaa acaaacgttt gatctggcca atcacaccct gcgcgcggct gcgggcacgt
     4141 gccagtgggg tgttggtagt catcatgcgg gtttcatcac ccgccgaata gtgctgccca
     4201 ggcccgcttt gtcctgcacc tgattgcttc cagaagggcg tagaaccaga aggttgcgca
     4261 ctctgcggac cgcctgcatc acgcgtgctg ttgtgtccag cggagccgcc agaggcattt
     4321 tcagctggtg cagaatttcc ccagcccagt cgcccaccaa agaatccacc agaccgctgc
     4381 gcagcttcct gtggctgccc gctgtagccc tgctgaccac tccgatgcgc ctgcgcactg
     4441 ccgccttggt tgcgatcctg tggatagccg ccgccgtatc ccaagtcccc caatccactc
     4501 gaatgtggat cctgggcttg ctggggtgta ccgggcggcg gtgccgaggg acgcggtcgc
     4561 ggtcccgcgg cacggactgg cgcccgtacc cgatccttta actcgatcgt tggcaacaca
     4621 cccttgctga tcatcaaggt gttgccgttc ttgtaggctt cgttcaaact ggtaatgagt
     4681 ttggcctgcg ccgtgtcgtt taccaggggc caagagtccc cgggcatgcc ggactgtgcc
     4741 cactgctcta ccatctggag aaccagaacc tccgggcgca atatgtcgcc cccgtccaga
     4801 tcatccagat tttcgagtag cttgatacgg acgcgcaagt cgtccagctg ggcaaaaacc
     4861 ttgtcgttca cggtcatcac cagacgggaa gccagaattt tgttctcgac aacctctgtt
     4921 cctaccaact caagacctgc cgcttccaga ctgttcttgg gcttggcgtc ttttgtaggc
     4981 tctagacaag tgcgccaggc tttcagtgtg ttttccagcc agagtggcct tacgcgcttg
     5041 taggccatcc atacatcgcg ccgaacctgg gactcacggg cattgcaagg ttcatccacc
     5101 agctccgtca accgcacttg cacctgcacg ctgagttcgg acatggcacg acccatttcc
     5161 gccacaaagc gctcccgcac ggcacgtgcc aactgcaggc ggggagagag tgtggaaggg
     5221 gtcgccatat atcttgaacc tagaccgtcg ccccggcatt ggggtcattg gggtcttcct
     5281 ctttcttggc cgtagccttg atgaggtctt cccgtgcgat acccagccac atcgcgatcg
     5341 ccgcagcgac gaacaccgac gagtaaatgc caaagagaat gccaatggtc aacgccagcg
     5401 caaagtagtg cagggtggcg ccgccaaaca ggagcattga aagcaccatg agctgtgtcg
     5461 atccatgcgt aatgatggtc cggctgatgg tggaagtgat cgcgttatca atgatctcca
     5521 ccgtattcat cttgcgatac cggcgaaagt tctcgcgaat ccggtcgaag atgaccaccg
     5581 attcattcac ggaatacccc agcaccgcca gcaccgcagc cagcaccggc agcgaaaact
     5641 cccactggaa gaatgcaaaa aatcccaaaa tgatgatcac gtcgtgcaaa ttggcaatga
     5701 tggcggccac ggcaaacttc cactcaaagc gaatggcgag gtagagcatg atgcccacca
     5761 ccacgaaggc cagagccttg agaccgtccg cggcaagctc atcccccacc tgtggaccta
     5821 caaactcggt gcgccgcagc gaagcggtcg cattgtcggc tttgagcgcc gccagaacct
     5881 gttcgctttg ctgaccggaa ctgctgcctt tttgcaccgg catgcgaatc agcacatcct
     5941 gtgccgtgcc aaagttctgc acctgcacat cggtcatccc cagcttcgca accgtggccc
     6001 gaatggaatt gaggtccgca ggctgcgaat agctcacctc catcagcgtg ccaccagtga
     6061 actccaccga cagatgcaga ccacgggaaa agaggaagaa gaccgccgcc aagaatgtgg
     6121 caagcg
//
LOCUS       DAOSJM010000123         6073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11742_length_6073_cov_4.372881, whole genome shotgun sequence.
ACCESSION   DAOSJM010000123 DAOSJM010000000
VERSION     DAOSJM010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11742_length_6073_cov_4.372881"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="PKX82_05800"
     CDS             complement(<1..601)
                     /locus_tag="PKX82_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOF51800.1"
                     /translation="MSKTILQNIPVGQKVGIAFSGGLDTSAALLWMKKKGALPYAYTA
                     NLGQPDESDYDEIPRKAMEYGAEKARLVDCRPQLAAEGIAALQSGAFHITTAGSTYFN
                     TTPLGRAVTGTMLVSAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPALKIYKPWLD
                     QQFIDELGGRAEMSAFMTANGFGYKMSAEKAYSTDSNMLG"
     gene            complement(686..1012)
                     /locus_tag="PKX82_05805"
     CDS             complement(686..1012)
                     /locus_tag="PKX82_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOF51801.1"
                     /translation="MTTTKIDGVSVTTAANVYFDGKCVSHGITFPDGTKKSVGVILPA
                     TLTFNTGAPEIMECVAGSCDYKLDGSDTWVTSGAGQKFSVPGNAKFQIRVTEGHAAYH
                     YICHFG"
     gene            complement(1090..1440)
                     /locus_tag="PKX82_05810"
     CDS             complement(1090..1440)
                     /locus_tag="PKX82_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HOF51802.1"
                     /translation="MTTLYGIPNCDTVKKARTWLTDHGVDYAFHDFKKQGVPQPQIDT
                     WLAAVGWETLINRKGTAWRGLDDATKAAVVDAASARAVAIASPSVIKRPVVQWADGTI
                     TVGFTPERFEQRLP"
     gene            1477..2742
                     /locus_tag="PKX82_05815"
     CDS             1477..2742
                     /locus_tag="PKX82_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HOF51803.1"
                     /translation="MTSHTVLAQPLTLPNGTVVKNRLLKSAMSEALGTREGSPTPELN
                     HLYKAWADGGIGLCVTGNVMIDLKARGEPGNVVIEDDTHLAALKAWAKAATAKNTHCW
                     VQLNHPGKQAPKGLNRETVAPSAVPFRDDMKAFFATPRELTDAEVQALVARFGKAAGI
                     VKQAGFSGVQIHGAHGYLVSQFLSPHHNVRTDDWGGTPEKRRRFVLEVYQAMRKAVGA
                     KFPIGIKLNSADFQRGGFTEEESLDTIRALADAGIDLIEISGGTYEAPAMTGVKTSKE
                     PVKESTRLREAYFLEFAEKARAAVKTPLVVTGGFRSAQGMAQAIESGAVDMVGLARML
                     AIEPDAPKKLLAGKPTLHTVKPITTGIKAIDKLGLLEISWYTGQLKRIGRGEPTKPRE
                     PGLWVFAKQAWHMAMAGKKKRPPAKLRAN"
     gene            3080..4399
                     /gene="folC"
                     /locus_tag="PKX82_05820"
     CDS             3080..4399
                     /gene="folC"
                     /locus_tag="PKX82_05820"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HOF51804.1"
                     /translation="METPTTLSDWLAHCERLHPKGINGIELGLDRVRSVAQRLNLHFT
                     CPVITVAGTNGKGSTCAMLESILGQAGYKTGVYTSPHLVHFEERMRLLGVASNAIKFV
                     AACADVERARGQNGSEILLTYFEFTTLAILLALSREELDVVILEVGLGGRLDAVNIVD
                     TDCAIITSIDLDHTELLGDTREAIGFEKAGIMRTGRPVVVSDPVPPQSVLDHALEVGA
                     DLWQVGKDFNVSGDKQQWGWAGRGRRYSGLAYPALRGANQLVNASGVLAALTALRERL
                     PVTAQAVRNGLAFVELPGRFQIIPGEPTLVLDVAHNPHSVAALVANLDAMGYAPTTHA
                     VFGAMADKDLAPMLARINPLIDRWYFTDLPTPRAASGATLQERWAAQNTRKEATASAW
                     PDPMAALQAAMSAADPTDRIVVFGSFYTVGGVLTHGIPRLQAKHLNP"
     gene            4411..5172
                     /locus_tag="PKX82_05825"
     CDS             4411..5172
                     /locus_tag="PKX82_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOF51805.1"
                     /translation="MAFFKFRKGGDEHPTPAPAPESVEALRKRARHRLIGAAILVLLG
                     VVGFPLLFDNQPRPIAVDIPIEIPDKTKVKPLTSVPPVASATVPAAAPVQASAAPAAP
                     ASRAVAGVVSAPPTAAAPKPAAKPEVKVEAKPEVKPEVKPQPKPLDKPTDAAKAQALL
                     DGKDAAKKPEAAASAASAGRFIVQIGAYAEVLKAREARQKLEKAGIKTYTQVVQTPEG
                     KRIRVRVGPFESKAEADKAAAKIKKLDLPAAILEL"
     gene            5188..5676
                     /locus_tag="PKX82_05830"
     CDS             5188..5676
                     /locus_tag="PKX82_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOF51806.1"
                     /translation="MPVLDWIFLGVLLLSLAVGAWRGLVYEVLSVLSWIAAFVLAQWF
                     APQAAQWLPMSGAAESMRYAAGFVLVFILAIFAGGLIAFLTKKLIAAVGLRPADRMLG
                     AAFGLVRGIIILLALTVVVGMTPMRSAQVWQESTGAHIASVALTGLKPVLPQEFGKYL
                     PS"
     gene            5687..>6073
                     /locus_tag="PKX82_05835"
     CDS             5687..>6073
                     /locus_tag="PKX82_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOF51807.1"
                     /translation="MCGIVGVVSNAPVNQLIYDALLLLQHRGQDAAGIVTQQERKFFM
                     HKAKGMVKDVFRTRNMRSLPGNCGLGQVRYPTAGNAFSEEEAQPFYVNAPFGIVLVHN
                     GNLTNAQALKAELFNTDHRHINTESDS"
BASE COUNT         1104 a         1902 c         1951 g         1116 t
ORIGIN      
        1 cgcccagcat gttgctgtcg gtgctgtagg ccttctcggc gctcatcttg tagccaaaac
       61 cgttggccgt catgaaggcg gacatttcgg cacggccacc gagttcgtcg atgaactgct
      121 ggtccagcca aggcttgtaa atcttcagcg cggggttggt cagcaggccg tagcggtaaa
      181 agcgctcgat gtcattgccc ttgaaggtgg aaccgtcgcc ccagatgttg acgtcgtctt
      241 ccttcatggc gctcaccagc atggtgccgg tgacagcgcg gcccagcggc gtggtgttga
      301 agtaggttga gccagccgtg gtgatgtgga aggcaccgct ttgcagcgcg gcaatgcctt
      361 cagcagccag ctgcgggcgg cagtccacca ggcgggcttt ttcggcgccg tattccatgg
      421 ccttgcgcgg aatctcgtcg tagtcgctct cgtcgggctg gcccaggttg gcggtgtagg
      481 cgtagggcag ggcgcccttc ttcttcatcc acagcagggc tgcgctggtg tccaggccac
      541 cggagaacgc gataccgacc ttttggccga cgggaatgtt ttgcagaatg gtttttgaca
      601 tgaaatgtgc ccttagcccg cgtggaatgt acgcaagcag ctattaaaaa tgtagcggtt
      661 cagtagaggc tgcagcggca gcagatcaac cgaaatggca gatgtagtgg tacgcggcgt
      721 ggccctcggt cacgcggatc tggaacttgg cgttgcccgg cacgctgaat ttctggcccg
      781 cgccgctggt gacccaggtg tcgctgccgt cgagcttgta gtcgcagctg ccagccacgc
      841 attccatgat ttcaggtgcg ccagtgttga aggtcagggt ggcgggcagg atcacgccga
      901 cggacttctt ggtgccatcg ggaaaggtga tgccatggct gacgcatttg ccgtcaaagt
      961 acacattggc tgcggtggtg acggaaacgc cgtcgatttt ggtggtggtc atggtgtgca
     1021 gtggtttgct ggatgagaaa aatggagccg tgccagccag cgtgctggcg gcaggcgtaa
     1081 cccctgattt tagggcagtc gctgttcgaa gcgttccggg gtaaagccca cggtgatcgt
     1141 gccatcggcc cactggacca ccgggcgctt gatgacactg gggctcgcga tggccacagc
     1201 gcgggcgctg gcggcatcca ccacggcggc tttggtcgca tcgtccagac cgcgccaggc
     1261 cgtgcctttg cggttgatca gcgtttccca gcccacggca gccagccagg tgtcgatctg
     1321 cggctggggc acgccctgct tcttgaagtc gtgaaacgcg tagtccacgc catggtcggt
     1381 cagccaggta cgggcttttt tgacggtgtc gcagttgggg atgccgtaaa gggtagtcat
     1441 gcgccagatt ttaggccgtg tggtccaatt cggcccatga cttcccacac cgtactcgcc
     1501 cagccgctga ccctgcccaa tggcacggtc gtcaagaacc gtttgctcaa atcagccatg
     1561 tccgaagccc tgggcacccg cgaaggctcg cccacgcccg agctcaacca cctctacaag
     1621 gcctgggccg atggcggcat tggcctgtgc gtcaccggca acgtgatgat cgacctcaag
     1681 gcccggggcg agcccggcaa tgtggtgatc gaggacgaca cccatctggc tgcactcaaa
     1741 gcctgggcca aggccgccac ggccaaaaac acccactgct gggtgcagct caaccacccc
     1801 ggcaagcagg cgcccaaggg cctgaaccgc gaaaccgtgg cgccctcggc cgtgccgttc
     1861 cgggacgaca tgaaggcctt ctttgccacc ccgcgcgaac tgaccgatgc cgaggtgcag
     1921 gcgctggtgg cgcgctttgg caaggctgct ggcatcgtca agcaggccgg gttcagcggt
     1981 gtgcagatcc acggcgccca tggttacctg gtgagccagt tcctctcgcc ccaccacaac
     2041 gtgcgcaccg acgactgggg cggcaccccc gagaagcgcc gccgctttgt gctggaggtg
     2101 taccaggcca tgcgcaaggc cgtgggagcc aagttcccga ttggcatcaa gctcaactcc
     2161 gccgacttcc agcgcggggg ctttaccgaa gaagagtccc tggacaccat ccgcgcgctg
     2221 gccgatgcgg gtatcgacct gatcgagatc tcgggcggca cctacgaggc ccccgccatg
     2281 accggggtga agaccagcaa agagccggtc aaggaaagca cccgcctgcg cgaggcctac
     2341 tttctggaat tcgccgagaa ggcccgagcc gcagtgaaaa ccccgctggt ggtaaccggc
     2401 ggtttccgtt ccgcccaggg catggcccag gccatcgaat ccggcgcggt ggacatggtg
     2461 ggcctggccc gcatgctggc cattgagccc gatgcgccca aaaagctgct ggccggcaag
     2521 cccacgctgc acacggtcaa gccgatcacc accggcatca aggccatcga caagctgggt
     2581 ctgctggaga tcagctggta caccggccag ctcaagcgca ttggccgcgg cgagcccacc
     2641 aaacccaggg agccggggct gtgggtgttt gccaagcagg cctggcacat ggccatggcc
     2701 ggcaaaaaga agcggccgcc tgcgaaattg cgggctaact gactcttcct gcgcctgcgt
     2761 tgctgagccc gcagcaagac gggggtgcac gcctccgttc gtgtcccccg gcctgcggcc
     2821 tcctccttta cctcactccg acgtacaccc ccatctcgct gctacgaacg ggtttgatgg
     2881 acgtcggata gcgagtcata ccacgctgga tcgggtgggt tgggcgttct gcgcaggtaa
     2941 aggaggaggc cgcaggccgg gggacacgga gcagaacgtc cggcccgccc ggtcccgcag
     3001 cacaacaagc cctgcaaatc ccacgctaac cagtttcgga atcactcagt cgcccatggt
     3061 cggcgacaat gctcccctca tggaaacacc cacaacgcta tctgactggc tcgcccactg
     3121 cgagcgcctg caccccaagg gcattaacgg catcgaactg ggcctggacc gcgtgcgcag
     3181 cgtcgcgcag cgcctgaatc tgcacttcac ctgcccggtc atcaccgtgg ccggcaccaa
     3241 tggcaaaggc tccacctgcg ccatgctgga atccattctg ggccaggccg gctacaagac
     3301 cggcgtttac acctcgcccc atctggtgca cttcgaggag cgcatgcgcc tgctgggcgt
     3361 ggcctcgaat gctatcaaat ttgtagctgc ctgcgcagat gtggagaggg ctaggggcca
     3421 aaatggttct gaaatcctgc tgacctactt cgaattcacc accctggcca ttctgctggc
     3481 cctgtcgcgc gaagaactgg atgtggtgat tctggaggtc ggtttgggcg ggcggttgga
     3541 tgcggtcaac attgtcgata ccgactgcgc gatcatcacc agcatcgacc tggaccacac
     3601 cgaattgctg ggcgacaccc gcgaagccat tgggttcgag aaagccggca tcatgcgcac
     3661 tggccgcccg gtggtggtga gcgacccggt gccgccccaa agcgtgctcg accatgcgct
     3721 tgaagtgggc gctgacctct ggcaggtggg caaggatttc aatgtgtccg gtgacaagca
     3781 gcaatggggc tgggccgggc gcgggcggcg ctacagcggg ctggcctacc ccgcattgcg
     3841 cggcgccaac cagctggtca atgcctcggg cgtgctggcg gcgctgacgg cgctacgcga
     3901 acgcctgccg gtgaccgccc aggcggtgcg caacgggctg gcgtttgtgg agctgcccgg
     3961 gcgtttccag atcattccgg gcgagcccac actggtgctg gatgtggcgc acaaccccca
     4021 ttcggtggcg gcgctggtgg ccaatctgga cgccatgggc tacgccccca ccactcacgc
     4081 ggtgttcggt gccatggccg acaaggacct ggcgcccatg ctggcgcgca tcaacccgct
     4141 gattgaccgc tggtacttca ccgacctgcc cacgccgcgc gccgccagcg gtgccacgct
     4201 gcaggagcgc tgggcggccc aaaatacccg caaagaagcc acggcctccg cctggccgga
     4261 ccccatggca gcactgcagg cggccatgtc tgcggcagac cccactgata gaatcgtggt
     4321 ctttgggtcc ttctacaccg tgggtggcgt gctgacacac ggtatccccc ggctgcaagc
     4381 caaacatctg aacccctaag tcacccatcc atggcctttt tcaagtttcg caaaggtggc
     4441 gacgagcatc ccacccccgc cccggctcct gaaagcgttg aggccctgcg caaacgcgcc
     4501 cgccaccgcc tgattggtgc cgcgattctg gtgctgctgg gcgtggtcgg tttcccgctg
     4561 ctgtttgaca accagccgcg tccgattgcg gtggatattc cgatcgaaat tccggacaag
     4621 accaaggtca agccactcac ctctgtgcca ccggttgcgt ctgccactgt gcccgctgct
     4681 gcgcctgtgc aggccagtgc cgcgccggca gccccggctt cccgtgcggt ggccggtgta
     4741 gtgagcgcgc cgccgactgc agctgcaccc aagcccgctg ccaagccgga agtcaaggtg
     4801 gaagccaaac ctgaggttaa gccagaagtc aagccacagc ccaagccctt agacaagccg
     4861 accgatgccg ccaaggccca ggcgctgctg gacggcaaag acgccgccaa gaagcctgaa
     4921 gcggctgcgt ctgccgcatc ggccggacgt ttcatcgtgc agattggcgc atatgccgag
     4981 gtgctcaagg cccgcgaggc ccgccagaag ctggagaaag ccggcatcaa gacttacacc
     5041 caggtagtac agacgcccga aggcaagcgt atccgtgtgc gcgtgggtcc gtttgagtcg
     5101 aaggccgagg ctgacaaggc ggctgcaaag atcaagaagc tcgatctgcc cgcagcgatt
     5161 ctggaactgt aaaggcggag gctgaacatg ccggtgctgg actggatctt tctgggggtg
     5221 ctgctgctgt cgctggcggt gggggcctgg cgtggactgg tctacgaggt gctgtcggtg
     5281 ttgagctgga ttgcggcttt tgtgctggcg cagtggtttg cgccgcaggc cgcgcagtgg
     5341 ctgccgatgt ccggggctgc ggagtcgatg cgctatgcag ctggttttgt tctggtcttc
     5401 atcctggcta tttttgcagg tggcctgatt gcatttttga ccaagaaact gattgcggcg
     5461 gtggggctgc ggcccgcgga ccgcatgctg ggcgcggcct ttggtctggt gcgcggcatc
     5521 atcattttgc tggcgctgac agtggttgtg ggcatgacgc ccatgcggtc ggcccaggta
     5581 tggcaggagt ccacgggcgc ccacattgcc agcgtggcat tgacgggcct gaaacccgtg
     5641 ctgccgcaag agttcggaaa atatctacct tcgtgagtaa acatctatgt gcggaatagt
     5701 cggcgttgtc agcaacgcac cggtcaacca gctgatctac gacgcgttgc tgctgctgca
     5761 gcaccgcgga caggacgcag cgggtatcgt cacccagcag gaacgcaagt tcttcatgca
     5821 caaggccaag ggcatggtga aggacgtgtt ccgcacgcgc aacatgcgct cgctgcccgg
     5881 caactgtggt ctgggccagg tgcgctaccc cacggcaggt aatgctttca gtgaagaaga
     5941 ggctcagccg ttttacgtga acgcgccttt tggcatcgtg ctggtgcaca acggcaacct
     6001 gaccaatgcg caggcgctca aagccgagct gttcaatacc gaccaccgcc acatcaacac
     6061 cgagagcgat tcg
//
LOCUS       DAOSJM010000124         6043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11821_length_6043_cov_3.790414, whole genome shotgun sequence.
ACCESSION   DAOSJM010000124 DAOSJM010000000
VERSION     DAOSJM010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6043
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11821_length_6043_cov_3.790414"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PKX82_05840"
     CDS             <1..266
                     /locus_tag="PKX82_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOF51808.1"
                     /translation="SAALGFPIALANAAGYAISGHGLAGLPAWSLGYIWLPALAVIAA
                     CSVTTAPLGAKMAHSLPVAKLKRVFALVLYCLAAYMLWKGLHG"
     gene            complement(301..1629)
                     /locus_tag="PKX82_05845"
     CDS             complement(301..1629)
                     /locus_tag="PKX82_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOF51809.1"
                     /translation="MSTPREASELIEVRFLRIGMYVELDVGWLAHPFPTGSFRIISEK
                     QIDTIRGLGQARIRWVPSRSELTATEAAQTPGAAQAASQAGEREEAQRREAQARQERA
                     DLLAAQQRSLTVCEKRFNEATRLYRKTVEQVHSHPKEAAEQAHSMVAGLLQDMLGEGD
                     SIIRLLSESAGDKSAMHPVNVTIVSLLLGKAMGMPRDALQDLGTAAYLHDIGKIQLPE
                     RVRWFEDNFSSAEYKLYQDHVAQSVLLGKGMSLSRAALLAIAQHHELADGTGFPARIK
                     SESMSPAARILALVNRYDNLCNPSRVATALTPHESLALIFAQMKTRFDSVALSAFIRM
                     MGVYPPGSVVQLIDDRYAIVVSVNSSRPLKPRILVHEPGVPRHEALIVDLEQMPFLGI
                     RRSLKPSALPQASLDYLAPRQRITYYFEQVEAPLAPQSGSTEPEAPKSDA"
     gene            complement(1626..2579)
                     /locus_tag="PKX82_05850"
     CDS             complement(1626..2579)
                     /locus_tag="PKX82_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HOF51810.1"
                     /translation="MSDILTVTLNPALDVSAITERVRPTSKLRCDAVQRHPGGGGINV
                     ARVLHRLGAECQAVCLLGGPSGQMLSQLLEQEGVPCVPVTIAGHTRESFTVLESASGQ
                     EFRFVLPGPDIHPDEIQTLMATLARQAPPKLVVGSGSLPPGVTTDFYAQLAQVAQGWG
                     ARMVVDGSGPAVAAALAQGVYMVKPSLREMRELTSLPLADLGAVREAALAWIADRRAE
                     VVAVSLGDRGAMLVTASCVLFAPPLPVQVISAVGAGDSFVAGMVWALSHGHPVERAFA
                     YAVAAGSAALSSTGTALCSASDVHRVVEHVQLLTEIPELRF"
     gene            complement(2678..3415)
                     /gene="phbB"
                     /locus_tag="PKX82_05855"
     CDS             complement(2678..3415)
                     /gene="phbB"
                     /locus_tag="PKX82_05855"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HOF51811.1"
                     /translation="MGQKVAYVTGGMGGIGTAICQRLHKEGFKVIAGCGPTRDHVKWI
                     AEQAALGYTFYASAGNVGDWDSTVEAFTKTKAEHGTIDVLVNNAGITRDRMFLKMSRE
                     DWDAVIETNLNSMFNVTKQVVADMVEKGWGRIINISSVNGEKGQAGQTNYSAAKAGMH
                     GFSMALAQELATKGVTVNTVSPGYIGTDMVNAIREDVLAKIVATVPVKRLGQPSEIAS
                     IIAWLASEEGGYATGADFSVNGGLHMG"
     gene            complement(3501..4679)
                     /locus_tag="PKX82_05860"
     CDS             complement(3501..4679)
                     /locus_tag="PKX82_05860"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOF51812.1"
                     /translation="MEDIVIVAAARTAVGKFGGTLAKTPATELGAAVIKGLLERTKLG
                     ADQIGEVILGQVLAAGVGQNPARQSLIKSGIAKETPALTINAVCGSGLKAVMLAAQAV
                     AYGDSDIVIAGGQENMSASPHVLLGSRDGQRMGDWKMIDSMIVDGLWDVYNQYHMGIT
                     AENVAKAHGITRDMQDALALASQQKAAAAQDAGRFVDEIVPFSIAQKKGDPIVFAADE
                     FINRKSNAEALAGLRPAFDKAGGVTAGNASGINDGAAAVMVMTAKKAAALGLKPLGRI
                     ASFATSGLDPAMMGMGPVPASQKALARAGWKAQDLDLLEINEAFAAQACAVNNAMDWD
                     TSKVNVNGGAIAIGHPIGASGCRILVTLLHEMQRRDAKKGIASLCIGGGMGVALTIER
                     "
     gene            complement(4710..>6043)
                     /gene="phaC"
                     /locus_tag="PKX82_05865"
     CDS             complement(4710..>6043)
                     /gene="phaC"
                     /locus_tag="PKX82_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOF51813.1"
                     /translation="QQWVDAASPANFLATNPEAIAKAVETRGESITQGMQLLWKDIEQ
                     GHMSQTDESVFEVGRNVATSEGAVVYENALFQLIEYKPLTAKVHERPMLFVPPCINKY
                     YILDLQPENSVIRYTVENGHRVFVVSWRNPDESLAATTWDDYIEQGPIKAIEVVQDIT
                     GAPTINTLGFCVGGTILSTALAVLAARGEKPAASMTLLTTLLDFKDTGILDVFVDEAS
                     VKLREVTIGADAPGGPGLLKGKELATTFSFLRPNDLVWNYVVGNYLKGETPPPFDLLY
                     WNSDSTNLPGPYYAWYLRNTYLENNLIKPGKATVCGEKVDMRLVDTPVYIYGSREDHI
                     VPINASYASTQVLPGKKRFVMGASGHIAGVINPPAKNKRSHWIRADGKLPKTQAEWLK
                     GAVEYPGSWWTDWSQWLKGHAGKQIAAPKTYGKGKYKAIEAAPGRYVKAKA"
BASE COUNT         1064 a         1913 c         1883 g         1183 t
ORIGIN      
        1 ccagtgcggc gctcggtttt cctatcgccc tggccaatgc tgcgggctat gccatcagcg
       61 ggcatggtct ggcaggcctg ccggcctggt cgctgggcta catctggctg ccggccctgg
      121 ccgtgattgc cgcctgcagc gtgaccaccg cgccgctggg cgccaagatg gcccacagcc
      181 tgccggtggc caagctcaag cgggtgtttg ccctggtgct gtactgtctg gccgcctaca
      241 tgctctggaa gggcctgcac ggctagcgtc cggcaaggct gctaaaccac gtgcaggcga
      301 ctacgcgtcg gacttagggg cttcgggttc tgtcgacccg gattgtggcg ccagaggcgc
      361 ctccacctgc tcaaagtagt aggtaatgcg ctggcgcggc gccaggtagt cgagcgacgc
      421 ctgcggcagt gccgaaggct tgaggctgcg ccgaatcccc agaaaaggca tttgctccag
      481 gtccacgatc agggcttcat gccgtggcac acccggttcg tgcacgagga tgcggggctt
      541 gagtggccgc gaagaattga cggacaccac gatggcatag cggtcatcga tcaactgcac
      601 caccgaaccc ggcgggtaga cacccatcat gcggatgaag gcactgagtg caaccgagtc
      661 aaaccgcgtt ttcatctggg caaagatcag cgcgagcgac tcgtgcggcg tcagagctgt
      721 ggcgacgcgg ctggggttgc acaggttgtc gtagcggttg accagcgcca gaatgcgcgc
      781 ggctggcgac atggactccg acttgatccg tgccggaaag ccggtgccat cggccagttc
      841 atggtgctgt gcaatcgcca gcaaggccgc acgcgagaga ctcatgcctt tgccgagcaa
      901 cacgctctgt gccacgtggt cctggtacag cttgtactcc gccgaagaaa aattatcctc
      961 aaaccagcgg acacgctctg gcaactgaat cttgccaata tcgtgcagat aggccgcagt
     1021 ccccaggtcc tgcagcgcat cgcgcggcat acccatggcc ttgcccagca gcagcgacac
     1081 gatggtcacg ttgaccggat gcatcgcgga tttgtcgccc gcgctctcgg acagcaggcg
     1141 gatgatggaa tccccttcgc ccagcatgtc ctgcaacagg ccggccacca tgctgtgcgc
     1201 ctgctcggcc gcctctttgg gatgggaatg cacctgctcc acggtcttgc ggtacaggcg
     1261 cgtggcttca ttgaacctct tttcacagac agtcaggctg cgctgctgtg cggccagcaa
     1321 gtcggcccgc tcctggcgcg cctgggcttc ccgccgctgc gcctcttccc gctctcctgc
     1381 ctgcgatgcc gcctgggccg cacctggagt ctgcgccgcc tcagtagctg tcagttcgct
     1441 acgcgacgga acccagcgga tgcgggcctg gccgaggccg cggatggtgt cgatctgctt
     1501 ttccgaaata atcctgaaac tgccggtggg aaacggatgg gccagccagc caacatcgag
     1561 ctccacatac atgccaatgc gcaggaaccg aacctctatc agctcggatg cttctctggg
     1621 ggtactcaaa accgcaactc cggaatctcg gtcaacaatt gaacatgctc caccacacgg
     1681 tggacatcac tggccgagca cagggctgtg ccggtgctcg acagtgccgc gctgccggcc
     1741 gccacggcgt aggcaaaagc ccgttccacg ggatgcccgt gtgacagcgc ccacaccatg
     1801 ccagccacaa agctgtcgcc cgcccctacg gcactgatca cctgcaccgg tagcggtggt
     1861 gcaaacaaaa cacagcttgc cgtgaccagc atggcacccc ggtcgcccag cgacacggca
     1921 accacctcgg cgcggcggtc cgcaatccag gccaaggccg cctctcgcac tgcacccagg
     1981 tcggccagtg gcaggctggt cagctcgcgc atttcgcgca aactgggttt caccatgtac
     2041 acaccttgag ccagtgcagc ggcaacggca gggccggaac catccaccac catgcgagca
     2101 ccccaaccct gggccacctg ggccagctgg gcataaaagt cggtagtaac gccaggcggc
     2161 agactgccgc taccgaccac cagtttgggc ggcgcctggc gcgccagcgt tgccatcagg
     2221 gtctggattt catcgggatg gatgtctggt cccggcagga caaagcggaa ctcctgcccg
     2281 ctggccgatt ccaggacggt aaaactctcg cgggtatggc cggcaatggt caccggcacg
     2341 cagggcacgc cttcctgctc cagcaactgg gacagcatct gaccggaggg tccacccagg
     2401 aggcataccg cctgacactc tgcgcccagg cgatgcagca cgcgggctac attgataccg
     2461 cctccaccgg gatggcgctg caccgcatcg cagcgcagct tgctggtggg tcgcacacgc
     2521 tcggtgatgg cagacacatc cagtgcggga tttaaggtga cagtaaggat gtcgctcatt
     2581 ggggtgcaag gttatcacct tccaaagcga aaaaccccgc aaaccgtgac agtttgcggg
     2641 gtggatgcaa cacctcggca caaatgccgt gagtggatca gcccatgtgc aggccgccgt
     2701 tcacggagaa gtcagcgcca gtggcgtaac cgccttcttc agacgccagc cacgcaatga
     2761 tggaggcgat ttcgctgggt tggcccaggc gcttgacggg cacggtggcc acgatcttgg
     2821 ccagcacgtc ttcgcggatg gcgttgacca tgtcggtacc gatgtagccc gggctgaccg
     2881 tgttgacggt cacacccttg gtcgccagct cttgcgccag ggccatggag aaaccgtgca
     2941 tgccggcttt ggcggccgag tagttggttt gaccggcctg gcccttctcg ccattgaccg
     3001 aagagatgtt gatgatgcgg ccccagcctt tttcaaccat gtcggcaacc acttgcttgg
     3061 tcacattgaa catggagttc aggttggttt cgatcacggc atcccagtct tcgcgactca
     3121 tcttcaggaa catgcggtca cgggtgatac cggcgttgtt caccagcaca tcgatggtgc
     3181 cgtgctcggc cttggtcttg gtaaatgctt ccaccgtgga atcccagtca ccgacattgc
     3241 cagcggacgc atagaaggta tagcccaggg cagcttgctc tgcaatccac ttgacatggt
     3301 cacgggtagg gccgcagcct gcaatcactt taaaaccttc cttgtgcaga cgctggcaaa
     3361 tggcggtacc gatgccaccc atgccaccgg tgacgtatgc gactttttga cccattctga
     3421 ttctcctcgt ttgatttact tgctacaaaa acagtagcta ccagcgccca ttatccgggg
     3481 gctggaagcc aaaaacatgc ttaacgctcg atggtgaggg ccacgcccat gccaccgccg
     3541 atacacagac tggcaatacc cttcttcgca tcgcggcgct gcatctcgtg cagcagcgtg
     3601 accagaatgc ggcaaccgga cgcaccgatg gggtggccaa tggcgatggc accgccgttg
     3661 acgttgacct tgctggtatc ccagtccatg gcgttgttca cggcgcaggc ctgggcagca
     3721 aaggcttcgt tgatttccag caggtccagg tcctgggcct tccagccggc tcgggccagg
     3781 gctttttgcg acgcaggcac tgggcccata cccatcatgg cggggtcgag gccgctggtg
     3841 gcaaaactgg caatgcggcc caggggcttg aggcccaggg cagcggcttt cttggctgtc
     3901 atgaccatga cggcggcagc gccgtcgttg atgcccgagg cgttgccggc ggtcacacca
     3961 ccggccttgt cgaaagcggg gcgcaggccg gccagtgctt cggcgttgga cttgcggttg
     4021 atgaactcgt cagcggcaaa cacgatcgga tcgccctttt tctgcgcaat gctgaacggc
     4081 acgatttcgt cgacgaaacg gccggcgtcc tgcgcggcag ccgccttctg ctggctggcc
     4141 agggccagtg cgtcctgcat gtcgcgggtg atgccgtggg ccttggccac gttctcggcg
     4201 gtgatgccca tgtggtactg gttgtagacg tcccacagac catccacgat catggagtcg
     4261 atcatcttcc agtcgcccat gcgctggcca tcacggctgc ccagcagcac gtggggtgat
     4321 gcgctcatgt tttcctggcc gccagcgatc acgatgtcgc tgtcgccata ggccaccgcc
     4381 tgggctgcca gcatgacggc tttcagacct gagccgcaca cggcattgat ggtcagcgcg
     4441 ggtgtttcct tggcgatgcc gctcttgatc agcgattggc gcgccggatt ctggcccacg
     4501 cccgcagcga gtacctgacc caggatcact tcaccgatct ggtctgcgcc cagtttggtg
     4561 cgctccagca ggcccttgat gacggccgcg ccgagttcag tagcgggtgt cttggccagg
     4621 gtgccgccga acttgccgac ggcagtgcgg gctgctgcaa cgataacgat gtcttccatg
     4681 tctgtctcct tcagtgtcga tatgtgaaat tacgctttgg ccttgacgta gcggccggga
     4741 gccgcttcga tggccttgta tttgcctttg ccgtaggtct tgggcgctgc aatttgcttg
     4801 ccagcgtggc ccttgagcca ctgcgaccag tcagtccacc agcttccggg gtactccacc
     4861 gcacccttga gccactcggc ctgggtcttg ggcaacttgc catcggcacg aatccagtgg
     4921 ctgcgcttgt tcttggccgg tgggttgatg acgccggcga tgtggccaga cgctcccatc
     4981 acaaaacgct ttttgcccgg cagaacctgg gtcgatgcgt aggacgcatt gatcggcacg
     5041 atgtggtctt cgcgggagcc atagatgtac accggcgtgt ccaccaaacg catgtccacc
     5101 ttttcgccac acaccgtggc cttgcccggc ttgatgaggt tgttctccag gtaggtgttg
     5161 cgcaggtacc aggcgtagta cgggccaggc agattggtac tgtccgagtt ccagtacagc
     5221 aggtcgaacg gcggaggtgt ttcgcccttg aggtagttgc ccaccacgta gttccacacc
     5281 agatcgttgg gccgcaggaa gctgaaggtg gtggccagtt ccttgccctt gagcaggccg
     5341 gggccgccgg gcgcgtcggc gccgatggtc acttcgcgca gcttgacgct ggcctcgtcg
     5401 acgaacacgt ccaggatgcc ggtgtccttg aagtcgagca gcgtggtcag cagcgtcatc
     5461 gacgcggccg gcttctcgcc gcgcgcggcc agcacggcca gcgcggtgct gaggatggtg
     5521 ccgcccacgc agaagcccag ggtgttgatg gtgggcgcgc cggtgatgtc ctgcaccacc
     5581 tcgatggcct tgatcgggcc ctgctcgatg tagtcgtccc aggtggtggc ggccaggctt
     5641 tcatcgggat tgcgccagct caccacgaag acgcggtggc cgttctcgac ggtgtagcgg
     5701 atcaccgagt tctcgggctg caggtcgagg atgtagtact tgttgatgca gggtggcacg
     5761 aacagcatcg gccgctcgtg caccttggcg gtgagcggct tgtactcgat caattggaac
     5821 agcgcgttct cgtagaccac ggcgccttcg ctggtggcca cgttgcggcc cacctcgaac
     5881 acgctctcgt cggtctgcga catgtggccc tgctcgatgt ccttccacag cagctgcatg
     5941 ccctgggtga tgctctcacc gcgcgtttcc accgccttgg caatcgcctc agggttggtg
     6001 gccaggaagt tggccgggct ggccgcgtcc acccactgct gca
//
LOCUS       DAOSJM010000125         6039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11833_length_6039_cov_4.335060, whole genome shotgun sequence.
ACCESSION   DAOSJM010000125 DAOSJM010000000
VERSION     DAOSJM010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11833_length_6039_cov_4.335060"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /gene="phoR"
                     /locus_tag="PKX82_05870"
     CDS             complement(<1..1271)
                     /gene="phoR"
                     /locus_tag="PKX82_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HOF51814.1"
                     /translation="MFWRIVSFVLCQAIPAAAGWFLVDAGHRPVAAIVLALLGTYVWL
                     VLDALRGLRLLHWLRSGNTAEIPLGNGLWGEVFDRIRKLVRTKDRALGESEGRLQEFL
                     AALQASPNGVVLLDADGRIEWFNQTAASHFGFDVQRDLLQHFGNLVRDPGFANYFATR
                     NFGTDVVMPGREHSGAKPVKLSVHMHPYGEGRSLLLSRDITAVEQAEAMRRDFVANVS
                     HEIRTPLTVLAGFVETMQSLPLQPQEREQYLGLMAQQAQRMQTLVSDLLMLSRLEGSP
                     LPPAHDWVSVNTLMAQCQQEAHDFSAVLWKQPQSIHFEWDEGTEIAGSLSELHSAMFN
                     LIGNAIRYTPMDKAIQVSWHNSLDGGGVFRVKDHGQGIAPEHLPRLTERFYRVDRSRS
                     RETGGTGLGLAIVKHVTQRHGAELGIESVPGS"
     gene            complement(1290..2015)
                     /gene="phoB"
                     /locus_tag="PKX82_05875"
     CDS             complement(1290..2015)
                     /gene="phoB"
                     /locus_tag="PKX82_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HOF51815.1"
                     /translation="MRNMPAVLVVEDEPAIAELVAVNLRHNGFRTTWAMDSASAQREI
                     DAGVPDLILLDWMLPGESGLVLAKRWRTHPRTKDVPIIMLTARGDESDRVAGLDAGAD
                     DYIAKPFSTKELLARVRAVLRRRAPEPVGESVKVGELMLDPATHRVTFGAQPLKLGPT
                     EFKLLHFMMGSAERVHSRANLLDKVWGDHVYIEERTVDVHVKRLREAMAGAGCMIETV
                     RGAGYRLTARPESVVTQSEKAGA"
     gene            complement(2012..2713)
                     /gene="phoU"
                     /locus_tag="PKX82_05880"
     CDS             complement(2012..2713)
                     /gene="phoU"
                     /locus_tag="PKX82_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOF51816.1"
                     /translation="MPDKHLSTQFDSELTSVSSRVMELGGLVESQIRQAIYALSQFSV
                     EVANEVSANEARVNAMEVEIDHLLSSIIARRQPTARDLRLLIAISKTTANLERVGDEA
                     EKIARMVRSIIQSGAPRSLPSLELRVASDLASGLLNKALDAFARLDVNAAVSILKEDD
                     LIDAEFDGFVRKLVTYMMEDPRMISPSLDLLFLAKAIERIGDHAKNIAEFIIYVVKGE
                     DVRHSSMEKIESVVK"
     gene            complement(2726..3505)
                     /gene="pstB"
                     /locus_tag="PKX82_05885"
     CDS             complement(2726..3505)
                     /gene="pstB"
                     /locus_tag="PKX82_05885"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOF51817.1"
                     /translation="MQTTITAPEKTKISVKDLNFYYGKFHALKGINLDIPENKVTAFI
                     GPSGCGKSTLLRVFNRMFELYPEQRAQGQILLDGENLLTSKEDVALLRAKVGMVFQKP
                     TPFPMSIYDNIAFGVKLFENLNATDMEERVEWALRKSALWTEVKDKLHQSGSSLSGGQ
                     QQRLCIARGIAIKPEVLLLDEPCSALDPISTAKVEELIVELKEDYTVVIVTHNMQQAA
                     RCSDYTAYMYLGDLVEFGSTEQMFFKPTRKETEDYITGRFG"
     gene            complement(3542..4411)
                     /gene="pstA"
                     /locus_tag="PKX82_05890"
     CDS             complement(3542..4411)
                     /gene="pstA"
                     /locus_tag="PKX82_05890"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOF51818.1"
                     /translation="MSTVAATNHAARAAKFAQRKRVNVIATTLALGAMAFGLFWLFWI
                     LWETIRLGFEGMTFATLTQMTPPPNEEGGLANAIYGSFLMVLLATFVGTPIGIMAGIY
                     LAEFDTKSWIASTTRFVNDILLSAPSIVVGLFVYSVVVSRFKSFSGYAGILALALIVI
                     PVVIRTTENMLQLVPAGLREAAYALGAPKWKVILSITLKSARAGVITGVLLAVARIAG
                     ETAPLLFTALSNQFWTSSLSEPMASLPVTIFKFAMSPYENWQKLAWAGVFLITLAVLA
                     LNILARVLTRNKG"
     gene            complement(4408..5373)
                     /gene="pstC"
                     /locus_tag="PKX82_05895"
     CDS             complement(4408..5373)
                     /gene="pstC"
                     /locus_tag="PKX82_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOF51819.1"
                     /translation="MTQPPPVKRARSGPMADRIFGWLAKSAALLTLALLFAILLSLTI
                     SAWPAISKYGLGFLTSTKWDPVQEEFGGLVMIYGTIATSVIALLIAVPVSFGIALFLT
                     ELSPAWLKRPLGTAIELLAAVPSIVYGMWGLLVFGPVLATYVQQPLQEALTGIPYLGA
                     FVSGPPVGIGILSAGIILAIMIIPFISAVMRDVFEVTPPLLKESAYGLGATTWEVVSK
                     VVLPYTKTGVIGGIMLGLGRAIGETMAVTFVIGNFNQLDSLSLFQAANSITSALANEF
                     AEAGEGLHQASLMYLGLVLFFITFVVLSLSKLLLSQLRKSEGAKT"
     gene            complement(5455..>6039)
                     /gene="pstS"
                     /locus_tag="PKX82_05900"
     CDS             complement(5455..>6039)
                     /gene="pstS"
                     /locus_tag="PKX82_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HOF51820.1"
                     /translation="AIAQVRRADGSGTTFIFTNYLSKVNAEWKSKVGEGTAVNWPVGA
                     GGKGNEGVAAFVARLPNSMGYVEYSYVKQNKLSYAVMQNSAGNFVKPEDDTFKAAAAG
                     ADWNKSFYQILTNQPGKDAWPISGATFILMHTKQDKPANATEALKFFSWAYKNGEKAA
                     ADLDYVPMPKPVIAAIEKAWGEIKDASGKPVAFK"
BASE COUNT         1391 a         1812 c         1604 g         1232 t
ORIGIN      
        1 gatcccggca cgctttcaat ccccaactcg gctccgtggc gttgggtcac gtgtttcaca
       61 atcgccagcc ccaaaccggt accacccgtt tcacgtgacc ggcttctgtc aacgcgataa
      121 aaccgctcgg tcaaccgtgg cagatgctcc ggtgcaatgc cctggccgtg atctttcacc
      181 ctgaataccc cgccaccgtc gagcgagttg tgccagctaa cctggatagc tttgtccatc
      241 ggggtgtagc gtatggcatt gccgatcagg ttgaacatgg cactgtgcag ctcggacagg
      301 gaccctgcga tttcagtccc ttcatcccat tcaaaatgaa tggactgtgg ctgcttccac
      361 aacaccgcag aaaagtcgtg tgcctcctgc tggcattggg ccatcagcgt attcaccgac
      421 acccaatcat gggcaggagg cagtggactg ccttccagtc gcgacagcat cagcaaatcg
      481 ctcaccaggg tctgcatgcg ctgggcctgc tgtgccatca agcccaggta ttgctcccgt
      541 tcctgtggct gcagcggaag ggactgcatg gtttcgacaa aacccgccag caccgtcagt
      601 ggtgtgcgaa tttcatggga gacattggcc acaaagtcac ggcgcatcgc ctctgcctgc
      661 tcaaccgcag tgatatcgcg cgacagcagc aaactgcgcc cttccccata ggggtgcatg
      721 tgtaccgaaa gcttgacagg tttggccccc gagtgctccc gtcccggcat aaccacatca
      781 gtgccgaaat ttctggtggc gaagtagttg gcgaaacccg gatctcgcac cagattgcca
      841 aaatgctgca gcagatcccg ttgcacatca aagccaaaat gggatgccgc cgtctggttg
      901 aaccattcaa tccgcccgtc cgcatccagc aggaccaccc cgttgggcga ggcctgcaat
      961 gccgcaagaa actcctgcag tcggccctcg ctctccccta gcgcccggtc cttggtgcgc
     1021 accagcttgc gtatgcgatc aaacacctca ccccaaagac cattgcccaa aggaatttca
     1081 gccgtatttc ccgagcgcaa ccagtgcaaa agccgcaagc cacgcaaggc atccagaacc
     1141 agccacacgt aagtgcccag caatgccaaa acaatcgcgg caacagggcg atgccctgcg
     1201 tcaaccagaa accaccccgc cgcggccggg atggcctgac acaatacaaa cgatacgatt
     1261 cgccagaaca tgcgctaatt ctgaacgatt caggctccgg ctttttccga ttgggtaacc
     1321 acactctccg ggcgtgcagt caaacggtaa ccagcacccc gcacggtttc aatcatgcag
     1381 ccagctcccg ccatggcctc acgcaggcgt ttgacatgga catccacggt gcgctcctca
     1441 atgtacacat ggtcgcccca taccttgtcc agcagattgg ctcggctatg cacgcgctcc
     1501 gcgcttccca tcatgaaatg caggagcttg aactcggtgg gtcccagttt caaaggctgt
     1561 gcaccaaacg tcacccgatg cgtcgccgga tccagcataa gctcgcccac tttcacggac
     1621 tcccctaccg gttcgggcgc cctgcggcgc agcactgcac gcacgcgggc cagcagctcc
     1681 ttggtggaga acggcttggc gatgtaatcg tccgcgccgg catccagccc agccacgcga
     1741 tcagactcat ctccacgcgc tgtcagcatg ataataggca cgtccttggt acgcgggtgc
     1801 gtgcgccaac gcttggccag caccagccca ctttcacccg gaagcatcca gtccagcaag
     1861 atcaggtcgg gcactccagc atcgatttcg cgctgggcgc tggcactgtc catggcccag
     1921 gttgttcgaa aaccgttgtg ccgcaggttg acggcgacaa gctcggcgat ggccggttca
     1981 tcttccacca ccagtacggc aggcatgttt ctcatttcac caccgactca attttttcca
     2041 tactggagtg gcgtacatct tcgcccttta ccacatagat gatgaactcg gcgatgttct
     2101 tggcgtggtc cccaatgcgc tcaatggcct tggccaggaa cagcagatcc aggctgggag
     2161 agatcatgcg cgggtcttcc atcatgtaag tgacgagctt tcggacaaat ccgtcaaact
     2221 cggcatcaat cagatcatct tccttgagga tggacacagc cgcattgaca tccaagcggg
     2281 caaaggcatc gagcgccttg ttcaacaaac ccgacgccag atcggatgcc acgcgcaact
     2341 ccagtgaagg caaagagcgc ggtgcgccgc tttggatgat gctacgcacc atgcgggcaa
     2401 tcttttccgc ttcatcccct acacgctcca gattggcagt ggttttggaa atggcgatca
     2461 acaagcgcag gtcacgcgcc gtcggctggc ggcgggcaat gatggatgac aacagatggt
     2521 caatctccac ttccatggca ttgacacgcg cctcgttcgc ggacacttca ttcgcaactt
     2581 caacactgaa ctgcgacaaa gcgtagattg cctggcgaat ctgggattcg accagaccac
     2641 ccaactccat gacacggctg gaaaccgagg tcagctcaga atcaaactga gtagatagat
     2701 gtttatcggg catgtggtcc tctccttagc cgaaacggcc tgtaatgtag tcttctgtct
     2761 ctttgcgcgt gggcttgaag aacatctgct ctgtagatcc aaactccacc agatcgccca
     2821 gatacatgta ggcggtgtag tcactgcagc gggccgcctg ctgcatgttg tgcgtcacga
     2881 tgaccaccgt gtagtcttcc ttgagttcca caatcagctc ttccactttg gccgtcgaga
     2941 tcgggtccaa tgccgagcat ggctcgtcca gcagaagcac ttccggcttg atggcaattc
     3001 ctcgcgcgat gcacaaacgc tgctgctgtc cgccagacag gctgctacca ctctggtgaa
     3061 gcttgtcctt gacctcggtc cacaacgctg atttgcgcaa cgcccactcc acccgctctt
     3121 ccatatccgt ggcgttgagg ttttcaaaga gcttcacacc aaaggcaatg ttgtcgtaga
     3181 tggacatcgg gaacggcgtg ggcttctgga acaccatgcc gactttggca cgcaagaggg
     3241 caacgtcttc cttgctggtc aacaggttct cgccatcgag cagtatctgt ccctgggcgc
     3301 gctgttcggg atacagctcg aacatgcggt tgaatacgcg cagcaatgtc gatttgccac
     3361 agccggatgg accgatgaaa gccgtgacct tgttctccgg aatatccaga ttgatgcctt
     3421 tgagcgcatg gaactttccg tagtagaagt tcaggtcctt gaccgaaatt ttggtcttct
     3481 cgggcgctgt gatggtggtt tgcataggaa tatgggaagt tattgggact acagatgcca
     3541 atcagccttt gttgcgtgta agcacacgcg ccaggatgtt gagtgcaagc acggccaggg
     3601 tgataagaaa tacacctgcc caggcaagct tctgccagtt ctcgtagggg ctcatggcga
     3661 atttgaaaat cgtgaccggc agactggcca tgggctcgct cagactggag gtccagaact
     3721 ggttgctcaa tgctgtgaac agcagcggtg ccgtctcgcc cgcgatgcgg gccactgcca
     3781 gcagcacgcc ggtaatgacg cccgcacgtg cggacttgag cgtgatactc agaatcactt
     3841 tccacttggg tgcgcccaat gcatatgccg cttcgcgcag acctgccggc accagttgca
     3901 gcatattttc cgtcgtgcga atgaccaccg gaatgacgat caatgccagt gccagaatac
     3961 ctgcatagcc tgaaaacgac ttgaagcgcg acaccacgac agaatagaca aacagtccca
     4021 ccacgatgga cggtgccgac agcagaatgt cgttcacaaa gcgggttgtg gaagcaatcc
     4081 agctcttggt atcaaattcg gccaggtaaa taccggccat gattccgatg ggggtaccca
     4141 cgaacgtggc caataacacc atcaaaaacg agccataaat cgcattggcc aggccaccct
     4201 cttcattggg cggaggtgtc atctgtgtca acgttgcaaa agtcatccct tcgaagccca
     4261 gacgaatggt ttcccacagg atccagaaca accagaaaag accaaacgcc atggcaccaa
     4321 gcgccagcgt agttgctatg acattgaccc gcttgcgctg cgcgaatttc gccgcgcgtg
     4381 cggcatggtt tgttgcggca acggtactca tgtcttggct ccttcgctct tgcgcaactg
     4441 tgacagcaac agcttggaca gtgacagaac gacaaatgtg atgaagaaca gtaccaatcc
     4501 gaggtacatc agggaggcct ggtgcagacc ctcacccgcc tcggcaaatt cattggccag
     4561 tgccgaagtg atactgttgg ccgcttgaaa caggctcagg gaatccagct ggttgaagtt
     4621 gccgatgaca aaagtgacag ccatggtctc cccaatagca cgccccaagc ccagcatgat
     4681 gccgccaata acacccgtct tggtgtaagg cagaaccacc ttggaaacca cttcccatgt
     4741 cgtcgcaccc agcccatagg cagattcctt gagcagcggc ggagtcactt cgaacacatc
     4801 acgcatgacg gcagagatga agggaatgat catgatcgcc aggatgatgc cggcggacag
     4861 tatgccaata cccacgggcg ggccggatac gaaagcaccc aaatacggta tgcccgtcag
     4921 tgcctcctgc aagggctgct gcacataagt cgccagtacc gggccaaaca ccagaagacc
     4981 ccacatgcca tacacgatgg atggcaccgc agccagcaac tcaatcgcgg tccccaatgg
     5041 gcgtttcagc caggccggcg acaactctgt caggaacaat gcgataccaa aactgacggg
     5101 cacagcgatc aacagcgcaa tgacggacgt agcaatggtg ccgtaaatca tcacgagacc
     5161 gccaaattct tcctggaccg ggtcccattt ggtactggtc agaaaaccca gaccgtattt
     5221 gctgatggca ggccatgcac tgatcgtcaa cgacagcaaa atggcaaaca gcagcgccaa
     5281 tgtcagcagc gcagcactct tggccagcca gccaaaaata cggtccgcca tggggccaga
     5341 tcgggctctt ttgacaggag ggggttgggt catggcgcgt acgggctggt tgacaggaaa
     5401 cggagccggg gaatccccat gcgaaagaat ggatgacatg gggacgtcct cggtttactt
     5461 gaaggcgaca ggcttgccag aagcatcctt gatttcgccc caggctttct caatggcagc
     5521 aatcacgggc ttgggcatgg gcacatagtc cagatctgcc gcggcttttt caccattctt
     5581 gtaagcccag ctgaagaact tcagcgcttc agtggcgttg gcgggcttgt cctgcttggt
     5641 atgcatcagg atgaaggtgg cgccactgat gggccaggca tccttgccag gctggttggt
     5701 taggatctga tagaacgact tgttccagtc ggcacccgca gcggcagctt tgaaagtgtc
     5761 atcttcaggc ttcacgaagt tgccagccga attctgcatc actgcatagg acagcttgtt
     5821 ttgcttgaca taggaatact ccacataccc catggagttg ggcagacgtg ccacgaatgc
     5881 tgcaacacct tcgttgccct tgccacctgc acccacgggc cagttcacag ccgtaccttc
     5941 accgactttg gacttccact cggcattaac cttgctcagg taattggtaa agatgaaagt
     6001 ggttccagaa ccatcagcgc gacgcacttg ggcgatcgc
//
LOCUS       DAOSJM010000126         5997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11934_length_5997_cov_5.416190, whole genome shotgun sequence.
ACCESSION   DAOSJM010000126 DAOSJM010000000
VERSION     DAOSJM010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11934_length_5997_cov_5.416190"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..994)
                     /gene="gltA"
                     /locus_tag="PKX82_05905"
     CDS             complement(<1..994)
                     /gene="gltA"
                     /locus_tag="PKX82_05905"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HOF51821.1"
                     /translation="MKLSDNKATLSFSNGSPSVEMPVYQGSIGPDVIDIRKLYAQTGM
                     FTYDPGFLSTASCQSAITYIDGDKGELLYRGYPIEQLATNCDYLDTCYLLLKGELPNE
                     TQRGDFHKLVINHTMVNEQMQFFLRGFRRDAHPMAVLTGLVGALSAFYHDSTDINNPE
                     HRDVAAIRLIAKMPTLVAMAYKYGTGQPYMYPRNDLSYSGNFLRMMFGTPCEDYKVNP
                     VIERAMDRIFILHADHEQNASTSTVRLCGSSGTNPFAAIAAGVACLWGPAHGGANEAC
                     LNMLEDIQRQGGVAKVGQFMEQVKDKNSGVKLMGFGHRVYKNYDPRAKLMQETCD"
     gene            complement(1005..1283)
                     /locus_tag="PKX82_05910"
     CDS             complement(1005..1283)
                     /locus_tag="PKX82_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HOF51822.1"
                     /translation="MSEALIDERALSKLKWRCRRGLLENDLFIERFFQRFSTTLNVRQ
                     AQALGDLMDLSDNDLLDVHLARKSLEQVDARLDREDVREVLQMLREPL"
     gene            complement(1305..2009)
                     /locus_tag="PKX82_05915"
     CDS             complement(1305..2009)
                     /locus_tag="PKX82_05915"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOF51823.1"
                     /translation="MTKRIFSIYRYDPDKDVKPYMQDIEVELDGSERMLLDALMKLKA
                     IDPTISFRRSCREGVCGSDAMNINGKNGLACLTNMRTLPGKIVLKPLPGLPVIRDLIV
                     DMTQFFKQYNSIKPYLINDTVPPEKERLQSPEEREELNGLYECILCASCSTSCPSFWW
                     NPDKFVGPAGLLQAYRFIADSRDQATGERLDNLEDPYRLFRCHTIMNCVDVCPKGLNP
                     TKAIGKIKEMMVLRAV"
     gene            complement(2025..3815)
                     /gene="sdhA"
                     /locus_tag="PKX82_05920"
     CDS             complement(2025..3815)
                     /gene="sdhA"
                     /locus_tag="PKX82_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HOF51824.1"
                     /translation="MSYSVTKRKFDVVIVGAGGSGMRASLQLARAGLNVAVLSKVFPT
                     RSHTVAAQGGIGASLGNMNDDNWHYHFYDTVKGSDWLGDQDAIEFMCREAPKVVYDLE
                     HMGMPFDRNADGTIYQRPFGGHTANYGEKAVERACAAADRTGHAMLHTLYQQNVAAKT
                     SFFVEWMALDLIRDADGDVVGVTAMEMETGDVHILEAKTTLLATGGAGRIFAASTNAF
                     INTGDGLGMAARAGIPLEDMEFWQFHPTGVAGAGVLLTEGCRGEGAILRNANGERFME
                     RYAPTLKDLAPRDFVSRSMDQEIKEGRGCGPNKDYVLLDMTHLGAETIMKRLPSVFEI
                     GHNFANVDITKEPIPVVPTIHYQMGGIPTNVNGQVVVPANGIHNEVVNGLYAVGECSC
                     VSVHGANRLGTNSLLDLLVFGRAAGNHIVDFASRTKSHKPLPKDAADRTLARLSRLDN
                     ATNGEYAQDVANDLRSSMQLHAGVFRTQKSMDEGVVKIAALRERVNAIGLKDKSKVFN
                     TARIEALEVDNLIEAAQATIVSAAARHESRGAHTVNDYGDTAEHPNGRNDTEWHKHTL
                     WHSEGNRLSYKPVQMKPLTVDSVELKVRTF"
     gene            complement(3839..4204)
                     /gene="sdhD"
                     /locus_tag="PKX82_05925"
     CDS             complement(3839..4204)
                     /gene="sdhD"
                     /locus_tag="PKX82_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HOF51825.1"
                     /translation="MAVNFGSKRIVVGAHYGLRDWLAQRVTAVLMALFTLVLLAQVIL
                     FQGPIGYDKWAGIFSAQWMKALTFSVIVALLYHAWVGIRDIWMDYIKPVSVRLSLQVF
                     SIVWLVSCAGWAIQVLWRL"
     gene            complement(4239..4682)
                     /gene="sdhC"
                     /locus_tag="PKX82_05930"
     CDS             complement(4239..4682)
                     /gene="sdhC"
                     /locus_tag="PKX82_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HOF51826.1"
                     /translation="MSELASSSRAKRPEFRNIHALSDLPTYRLPPAGWVSILHRISGA
                     ILFLLMPFIVWMFDTSLSSEFSFAKFTSAFSNGVGFVPGWFFKLVALAILWAYLHHFI
                     AGVRHLWMDINHAVSKEFGRSSAIVTLSLSLGLTVVLGAKLFGLY"
     gene            complement(4797..5594)
                     /locus_tag="PKX82_05935"
     CDS             complement(4797..5594)
                     /locus_tag="PKX82_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOF51827.1"
                     /translation="MTTTPPSSSDSAATGGVEAGALPSTPAFSPLYQQIKVLMLQSLQ
                     SGEWKPSAAIPSEIELAARFRVSQGTVRKAIDELAAENLLVRRQGKGTFVATHAEQHV
                     QYRFLKLFPDSGDVNSEGPAQRQIIDCKRVRAAADVARALGLRQGDSVLQVRRVLSFA
                     KVPTILEDIWLPGTPFKGLTAERLRDYDGPMYALFETEFGVRMVRAEEKIRAILPDAE
                     QCELLRTSPQTPLLSVERTAYTYNDTPMEMRRGLYLTDTHYYRNELN"
     gene            5784..>5997
                     /locus_tag="PKX82_05940"
     CDS             5784..>5997
                     /locus_tag="PKX82_05940"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOF51828.1"
                     /translation="MSKKPVRVAVTGAAGQIGYALLFRIASGEMLGKDQPVILQLLEI
                     PDEKAQNALKGVIMELEDCAFPLLAGI"
BASE COUNT         1220 a         1629 c         1861 g         1287 t
ORIGIN      
        1 cgtcgcaagt ctcttgcatg agcttggcgc gtgggtcgta gttcttgtag acgcggtggc
       61 caaagcccat cagcttgacg ccggagttct tgtccttgac ctgttccatg aactggccaa
      121 ccttggcgac accaccctgg cgctggatgt cttccagcat gttcaggcag gcttcattgg
      181 cgccaccgtg ggcggggccc cacaggcagg ctacgcccgc agcgatagcc gcaaacggat
      241 tggtgccgga agaaccgcac agacgcactg tggaagtgga tgcgttttgt tcatggtctg
      301 cgtgcaggat gaagatgcgg tccatcgccc gttcgatcac ggggttgacc ttgtagtctt
      361 cgcatggtgt gccgaacatc atgcgcagga agttgcccga gtagctcagg tcgttgcgcg
      421 ggtacatgta aggctgtccc gtaccgtact tgtaggccat ggctacgagc gtaggcatct
      481 tggcaatcag gcgaatcgcc gctacgtcgc ggtgttcggg attgttgatg tcggtgctgt
      541 catggtagaa ggccgacagg gcgcctacca ggccggtcaa cacggccatg gggtgtgcat
      601 cacggcggaa gccacgcagg aaaaactgca tctgctcgtt gaccatggtg tggttgatca
      661 ccagcttgtg gaagtctcca cgctgtgttt cgttgggcaa ttcgcccttc agcagcaggt
      721 agcaagtgtc caggtaatcg cagttggtgg ccagctgctc gatcgggtag ccgcggtaca
      781 gcagttcacc cttgtcgcca tcaatgtagg tgatggcgga ctggcacgat gcggtcgaca
      841 ggaacccggg gtcgtatgta aacatgccgg tctgggcata cagcttgcgg atgtcgatga
      901 catcggggcc gatgctgccc tggtacacgg gcatttcgac gctagggctg ccattgctga
      961 acgacagggt ggctttgttg tcagatagtt tcatctttcg cctttcagag gggttctctc
     1021 aacatttgta aaacttcacg cacgtcttcg cggtccagcc gggcgtcaac ctgttcgagc
     1081 gactttcgtg ccagatggac atccagcaga tcgttgtcac tcaaatccat cagatcgcca
     1141 agggcctggg cctgcctgac gttcagggtg gtactaaacc tctggaaaaa ccgctcaatg
     1201 aacaggtcgt tttccagcaa accgcgacgg caacgccatt tcagtttgct cagggcccgt
     1261 tcatcgatca gtgcttcact cacgtgtgta tttccggtta cgcgttaaac agcgcgcaaa
     1321 accatcattt ccttgatctt gccgatggcc ttggtggggt tcaatccctt cgggcaaaca
     1381 tcgacgcagt tcatgatggt gtggcagcgg aacaggcggt aggggtcttc gaggttgtcg
     1441 agacgctcgc cagtggcctg gtcgcggctg tcggcaatga agcggtacgc ctgcaacagg
     1501 cctgcgggac ccacgaactt atccgggttc caccagaagc tggggcaact ggtggagcag
     1561 cttgcgcaca gaatgcactc gtacaggcca ttgagctctt cacgctcttc ggggctttgc
     1621 aggcgctctt tttcgggcgg aacggtatcg ttgatcaggt agggcttgat cgagttgtac
     1681 tgcttgaaga actgggtcat gtccacgatc agatcgcgga tgacaggcag gccaggcagg
     1741 ggctttagca cgatcttgcc gggcaaggtg cgcatattgg tcaggcaggc cagaccgttc
     1801 ttgccattga tgttcatggc atccgagccg cacacgcctt cacggcagga gcggcggaac
     1861 gagatggtcg gatcgatagc cttgagcttc atcagggcgt caagcagcat gcgctcgctg
     1921 ccatcgagtt ccacctcgat gtcctgcatg tagggcttga cgtccttgtc cggatcgtaa
     1981 cggtagatgg aaaaaatgcg tttggtcatg atctggatct cgctttagaa cgtacgaacc
     2041 ttgagttcca cagagtccac cgtcagaggt ttcatctgca cgggcttgta gctcaggcgg
     2101 ttgccttcgc tgtgccacag ggtgtgcttg tgccactcgg tgtcattgcg gccgttgggg
     2161 tgctctgccg tgtcaccgta gtcattgacg gtgtgggcgc cacggctctc gtggcgggct
     2221 gcggcagaga cgatggtggc ctgtgcggcc tcgatcaggt tgtcgacttc cagtgcctcg
     2281 atacgggcgg tgttgaatac cttggacttg tccttgagtc cgatcgcgtt gacacgctcg
     2341 cgcaatgcag cgatcttgac cacaccttcg tccatgctct tttgcgtgcg gaacacaccg
     2401 gcatgcaact gcatggagga acgcaggtcg ttggccacat cctgggcgta ttcgccgttg
     2461 gtggcattgt ccaggcgtga caggcgcgcc agagtgcggt ctgcggcgtc cttgggcaac
     2521 ggtttgtggg atttggtgcg ggaggcgaag tccacaatgt ggttaccagc ggcgcgacca
     2581 aagaccagca ggtccagcaa cgagttggtg cccagacggt tggcgccgtg cacgctgacg
     2641 caggagcatt cacccacggc gtagaggccg ttgacgactt cgttgtggat gccattggca
     2701 ggcaccacga cctggccgtt gacattggtg gggatgccgc ccatctggta gtggatggtg
     2761 ggaaccacag ggatcggctc cttggtgata tccacgttgg caaagttgtg gccgatctcg
     2821 aacaccgagg gcaggcgctt catgatggtc tcggcaccca ggtgggtcat gtcgagcaac
     2881 acgtagtcct tgttgggtcc gcaaccgcgg ccttccttga tttcctggtc catcgaacgg
     2941 gacacgaaat cgcgtggagc cagatccttc agcgtaggcg catagcgctc catgaagcgt
     3001 tcgccattgg cgttgcgcag gatggcgcct tcgccgcggc agccttcagt cagcaacacg
     3061 ccggcaccgg ccacgccggt ggggtggaac tgccagaatt ccatgtcttc cagcgggatg
     3121 cctgcacgtg cggccatgcc caggccatca ccggtgttga tgaaggcgtt ggttgaagcc
     3181 gcgaagatgc gacctgcgcc accggttgcc aacaatgtgg tcttggcttc gaggatgtgt
     3241 acatcgcccg tttccatttc catcgcagtc acgccgacca cgtcgccatc ggcatcgcgg
     3301 atcaggtcca gtgccatcca ttccacgaag aaactggttt tggcggccac gttttgctgg
     3361 tacagcgtat gcagcatggc gtgaccagta cggtcagcgg cggcgcaagc acgttccaca
     3421 gccttctcgc cgtagttggc ggtgtggcca ccgaacggac gctggtaaat ggtgccgtcg
     3481 gcattgcggt caaaaggcat gcccatgtgt tccaggtcgt acacaacctt gggtgcttca
     3541 cggcacatga attcgatggc gtcctggtcg cccagccagt cagaaccctt cacggtgtcg
     3601 tagaagtggt aatgccagtt gtcgtcgttc atattgccca acgaggcgcc gatgccgccc
     3661 tgcgccgcca cggtgtgcga gcgggtgggg aacactttgg agaggacggc gacgttcaaa
     3721 ccggcacggg ccaattgcag cgaagcgcgc atgccggaac caccggcgcc aacgatcact
     3781 acatcaaatt tgcgtttggt tactgagtaa gacattgatt acagcctgaa gtaggggatc
     3841 agagacgcca gagaacctgg atggcccagc cagcgcagct gaccagccag acgatggaaa
     3901 acacctgcag ggagaggcgc acggagaccg gtttgatgta gtccatccag atgtcccgaa
     3961 tgcctaccca ggcgtggtac agcagcgcca ctatgacgga gaatgtcagc gctttcatcc
     4021 attgggcgga aaaaatgccg gcccatttgt cgtagccgat cggaccctgg aagaggatga
     4081 cttgggccaa cagcaccaag gtgaagaggg ccatcaggac ggctgtcacg cgctgtgcca
     4141 gccagtcgcg cagaccgtaa tgcgcaccaa cgacgatgcg tttggagccg aaattaactg
     4201 ccatggtcag gttccttgtt atgtggttgt aaataggctt agtacaggcc gaacagcttg
     4261 gcgcccagaa cgacggtgag gcccaggctc aaagacagag tgacgatggc cgaactgcga
     4321 ccaaattcct tgctcacggc atggttgatg tccatccaga ggtggcgaac gcccgcgatg
     4381 aagtggtgca gataagccca cagaatggcc agtgcgacca gtttgaaaaa ccaaccaggt
     4441 acaaaaccga caccgttgct gaatgcactg gtgaatttag caaaagaaaa ctcggaagag
     4501 agcgaagtgt caaacatcca gacgatgaat ggcatcaaca ggaacaggat ggcgccactg
     4561 atgcggtgca ggatggacac ccatcccgcc ggggggaggc ggtaagtggg gaggtcactc
     4621 agcgcgtgga tgttgcgaaa ctctggccgt ttggcgcggg aggaagaggc aagctcagac
     4681 atggatgggc tttctggaag taactgcgtt gtaattttcg atgtaaaacc gctaaattct
     4741 attgcaccgc atcaatctga caatggactt gcatatttgc tgtgcatcaa ggggattcaa
     4801 tttagctcat ttcgatagta gtgggtgtct gtcaggtaaa ggcctctgcg catctccatg
     4861 ggcgtgtcgt tgtaggtgta tgcagtgcgc tccacgctca acaatggggt ttgtggtgat
     4921 gtgcgcagca gttcgcattg ctcggcgtcc ggaagaatgg cgcggatttt ttcctcggca
     4981 cgcaccatgc gtacaccgaa ttctgtttca aacagcgcat acatcgggcc gtcatagtcg
     5041 cgcaggcgct cggccgtcag gcccttgaag ggtgtacccg gcagccagat gtcttccaga
     5101 atagtgggga ccttggcgaa ggacagcacg cggcgtacct gcaacacgga gtcgccctgg
     5161 cgcagcccaa gggcacgcgc tacgtccgcg gcggcgcgca cgcgtttgca gtcaatgatc
     5221 tggcgttgtg cggggccttc gctgttgacg tcgccgctgt ccggaaacag cttcaggaat
     5281 cggtactgca catgctgctc agcatgggtg gcgacaaacg ttcccttgcc ctggcggcgc
     5341 accagaagat tctcggcagc cagttcatca atggctttgc gcactgtgcc ttggctcacg
     5401 cggaagcgcg ctgcaagttc aatttcgctg ggaatggcgg cgctcggttt ccattcgccg
     5461 gactgcaggc tttgcagcat cagcaccttg atttgctggt acagcggact gaatgcgggc
     5521 gttgacggca acgcgcccgc ttcaacgccg ccggtggcgg cgctgtcgct ggaagagggg
     5581 ggcgtggtgg tcatgaaaac gtcgaaaaag tcagcttcaa gtcagactgg aaattgtttg
     5641 caatcatatc ttatataaga cataagacaa attgacgccg cggccaaatc gggggtaaac
     5701 ttgcgcggat tctgcagggg tcgaccgtac cgtgtgggcg cctggacagc aacccttttc
     5761 acaaccttcc tggagtaatt accatgagca aaaagcctgt tcgcgtcgcc gttaccggag
     5821 cagctggtca aatcggatac gcccttctgt tccgcatcgc gtcgggcgaa atgctgggca
     5881 aagaccagcc cgtgattctg cagctgctgg aaatccctga cgagaaagcc cagaacgcgc
     5941 ttaagggcgt gatcatggaa ctcgaagact gcgccttccc cctgctggcc ggtatcg
//
LOCUS       DAOSJM010000127         5989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_11954_length_5989_cov_4.143242, whole genome shotgun sequence.
ACCESSION   DAOSJM010000127 DAOSJM010000000
VERSION     DAOSJM010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11954_length_5989_cov_4.143242"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PKX82_05945"
     CDS             complement(<1..172)
                     /locus_tag="PKX82_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOF51829.1"
                     /translation="MVAMSDAIPPSRPAGLLLTGGGARAAYQVGVLEAIADIRLACGA
                     GHEPNPFPIITGT"
     gene            238..1329
                     /gene="apbC"
                     /locus_tag="PKX82_05950"
     CDS             238..1329
                     /gene="apbC"
                     /locus_tag="PKX82_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HOF51830.1"
                     /translation="MTVTVQAITDAIKAVIDPNTGKDFVSTKAVKNLTVSDGDVAFDV
                     ELGYPAKSQIAGLRKALIAAAKGVAGVGNVSVNVTVNIVAHSVQRGVQLLPRVKNIVA
                     VASGKGGVGKSTTAVNLALALAAEGARVGLLDADIYGPSLPMMMGIEGRPESDDGQTM
                     DPLENYGVQVMSIGFLVAQDEAMIWRGPMATQALEQLLRQTNWKDLDYLIVDMPPGTG
                     DIQLTLSQRVPMTGAVVVTTPQDIALLDAKKGIKMFEKVGVPILGIVENMAVHVCSNC
                     GHVEHIFGADGGKKMAADYGMDYLGALPLNMQIRLQADSGKPTVVSDPDSEVAALYKA
                     VARKVAVSIAAKNKDFSSKFPTITISKNT"
     gene            1348..2292
                     /locus_tag="PKX82_05955"
     CDS             1348..2292
                     /locus_tag="PKX82_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOF51831.1"
                     /translation="MNLLSSLRYLVALNEHRHFGRAAQACHITQPALSNALRALESEF
                     NVVIVKRDRNFVGFTPEGERVLASAQLMLHEHAILQQELKSDIHLPRGPLRIAAVPTA
                     VPIAARFAAMLHARHGGIQPIVLSLSSAALEQGLEDLSIDLALGYSERMRVQDSRLRA
                     WPQYTEHYFLLRRAAQPHRDGLQIGTPVQWREVAAMPLCLLTAEMHNRTIVDAAFRAA
                     GVSVQPAMETNSIMTLMLSVVAGDVCSILPGSLVDTVRNYRELDALPLIGPEVRTPIA
                     FISHAQARPSRALEAALALLQDATWLQHAASHSGALTV"
     gene            2434..4071
                     /locus_tag="PKX82_05960"
     CDS             2434..4071
                     /locus_tag="PKX82_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HOF51832.1"
                     /translation="MAAPGILDKERIIAGPGFNRWLVPPAALAIHLCIGMAYGFSVFW
                     LPLSKALGIKEAIQCGPDVGFWGQLFTTACDWQISTLGWMYTLFFVLLGSSAAIWGSW
                     LEHAGPRKAGVVSALCWCGGMLVSALGVYLHQFWLMVLGSGVIGGIGLGLGYISPVST
                     LIKWFPDRRGMATGMAIMGFGGGAMIGSPLAADLMKYFATPTDVGVMQTFVVMGLVYF
                     VFMMAGAFGYRVPETGWKPAGWTPPAADAANTMITQRHVHSSKVWGIPQFWLIWMVLC
                     MNVSAGIGVIGMASPMLQEVFGGSLIGVAKKFGELDKAELGAIAAVAAGFTALLSLFN
                     IGGRFFWASLSDKLGRKLTYVVFFVLGGVLYFSVPGSAAAGSIVLFVAAFCIILSMYG
                     GGFATVPAYLADLFGTQMVGAIHGRLLTAWATAGVLGPVVVNYMREYQLGLGIPREQV
                     YNQTMYILTGMLVVGLICNLMVRPLADKWFMTDAELAEEKRLAHERAAASVVVRGAGA
                     GEDAVSPVVVALAWAAVGIPLAWGVYRTLLSVAKFFA"
     gene            4093..>5989
                     /locus_tag="PKX82_05965"
     CDS             4093..>5989
                     /locus_tag="PKX82_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOF51833.1"
                     /translation="MRSTSESPDIPTVALGSVDDMRERIRKKSKLKGRQADDASLAEV
                     RALLGPRPDGGHRRDLLIEHLHTLNDAYRCLHDRHLVALAKEMNLPMAEVYEVATFYH
                     HFEVVQGDAKAPGLTVRVCDGLSCEMAGAQNLLARLTDLLGSADVRVIAAPCLGRCEQ
                     APVAVVHQRPVLHASPEAVVSMINSAAAPEEYAQDATNFIANSYAEKSVSPLPADTVA
                     VPAYAGYDTYRAHGGYALVAELASGALRADAVLQTLEDSGLRGLGAAGFPAGRKWRIV
                     RDQPAPKLMAVNIDEGEPGTFKDRTYLERDPHRFLEGMLVAAQVVGIEAVYIYLRDEY
                     HGCRAILQAELAKLQANPPCELPHIELRRGAGAYICGEESAMIESIEGKRGEPRMRPP
                     YIAQVGLFGRPTLEHNFETLYWVRDIVEKGAAWFASFGRNGRKGLRSFSVSGRVRYPG
                     VKLAPAGITLRELVDEYCGGMADGHTLYAYLPGGASGGIFPAHLADLPLDFDTLQPYG
                     CFIGSAAVIVLGQHDRARDAALNTMRFFAHESCGQCTPCRVGTEKAARLMEAETWDAD
                     TLDDLAFVMADASICGLGQAAPNPIRCMHKFFPHEVGEGPWPGDLPKPRGKGARTERK
                     APLGTPPE"
BASE COUNT         1062 a         1788 c         1966 g         1173 t
ORIGIN      
        1 atgtgccggt aatgatgggg aacgggttgg gctcatgtcc ggcaccgcag gccagccgaa
       61 tgtcggcaat ggcttccagc acgcccacct gataggctgc gcgtgcgcca ccccccgtta
      121 gcaacagacc ggcaggtcgc gagggtggaa tggcgtcgga catggcaacc attatgcagg
      181 ggtacccgcg tggcaaaaag ggttggatag actatggaca tcttcaggag aaattgaatg
      241 acagtgacag tgcaagccat aacagacgcc atcaaggccg tcatcgaccc caataccggc
      301 aaagactttg tgtcgaccaa agccgtcaag aatctgaccg tgtccgatgg tgatgtagcg
      361 tttgacgtgg aacttggata ccccgccaag agccagatcg ccggcttgcg caaagcgctg
      421 attgccgctg ccaagggcgt tgccggcgtg ggcaatgtgt cggtcaacgt gacggtcaac
      481 atcgtggccc actcggtgca acggggcgtg caactgctgc cgcgtgtcaa gaatattgtg
      541 gccgttgcat ccggcaaagg cggtgtgggc aagagcacca cggctgtcaa cctggcgctg
      601 gcactggcgg ccgaaggtgc acgcgtgggc ctgctggacg cggatatcta tggccccagt
      661 ctgcccatga tgatgggcat tgaagggcgc cccgaatccg atgacggcca aaccatggac
      721 ccgctggaaa actatggcgt gcaggtcatg tccatcggct tcctggtggc gcaggacgaa
      781 gccatgatct ggcgcggccc catggccacc caggcgctcg aacagttgct gcgccagacc
      841 aactggaaag acctggatta cctgattgtc gacatgcccc cgggcacggg cgacatccag
      901 ctgaccctga gccagagggt gcccatgacg ggtgcggtgg tggtgaccac gccgcaggac
      961 attgccctgc tggatgccaa aaagggcatc aagatgttcg agaaggtagg ggtacccatt
     1021 ctgggcattg tggaaaacat ggcggtgcac gtgtgcagca actgcggcca tgtggagcac
     1081 atcttcggtg ccgacggtgg caagaagatg gcggccgact acggcatgga ctacctcggt
     1141 gccttgccat tgaacatgca gatccgtctg caggccgaca gtggcaaacc caccgtggtg
     1201 tcggaccccg acagcgaggt ggcggccctg tacaaggcgg tcgcgcgcaa ggtggcggtt
     1261 tccattgctg ccaaaaacaa ggacttttcg tccaagttcc cgaccatcac catcagcaag
     1321 aacacctgac cgcaacacgg cgcggccatg aacctgctgt cttcgctgcg ctacctggtg
     1381 gcgctgaacg aacacaggca ttttggtcgg gccgcacagg cctgccacat cacccagcct
     1441 gcgctgtcca atgcactgcg ggcgctggag agtgaattca atgtggtgat cgtcaagcgc
     1501 gaccgcaact tcgtcggatt cacgcccgag ggcgagcggg tgctggcctc ggcccagttg
     1561 atgttgcatg agcacgccat cctgcaacag gagctcaaga gcgatatcca ccttccgcgc
     1621 ggccccctgc ggattgccgc cgtaccgaca gccgtgccga ttgcggcccg ctttgcggcc
     1681 atgctgcacg cgcgccacgg tggcatccag cccatcgtgc tgtccctgag ttctgcggca
     1741 ctggaacagg ggctggaaga cctttccatc gatctggcgc tggggtacag cgagcgcatg
     1801 cgcgtgcagg acagccggtt gcgggcctgg ccgcagtaca ccgagcacta tttcctgttg
     1861 cgccgtgcgg cccagcctca ccgtgacggt ctgcagattg gtacccctgt gcagtggcgc
     1921 gaggttgcgg cgatgccttt gtgcctgttg actgccgaaa tgcacaaccg gaccatcgtc
     1981 gatgcagcat tcagagccgc aggcgtatcg gtacaaccgg ccatggaaac caattccatc
     2041 atgaccctga tgctcagcgt cgtggccggg gatgtgtgca gcatcctccc cggttcgctg
     2101 gtggacaccg tgcgcaacta ccgcgaactg gacgcgctgc ccttgattgg ccccgaagtg
     2161 cgcacgccga ttgctttcat ctcccatgcg caggcaagac cttcgcgcgc cctggaggcg
     2221 gcactggccc tgttgcagga cgccacctgg ctgcagcacg cagcgtccca cagtggcgca
     2281 ctgactgttt aacttgttgc gccgcgtcat ttagtttttg aatcatccca tgcgatgatt
     2341 caattggacg ggcgcgcaag ctccccggat actcgcgcca tgccgtagct gggcgtagaa
     2401 aaatagacaa acaccccact ggagacaaca accatggcag caccaggaat cctggacaag
     2461 gaacgcatca ttgccggccc cggcttcaac cggtggctgg tgccgcctgc agcgctggct
     2521 attcacctgt gcatcggcat ggcctacggc ttctcggtgt tctggctgcc gctgagcaag
     2581 gccctgggca tcaaggaagc cattcagtgc ggtcccgatg tgggcttttg gggccagttg
     2641 tttaccactg catgcgattg gcagatctcc acgctgggct ggatgtacac cctgttcttt
     2701 gtgctgctcg gttccagtgc cgccatctgg ggcagctggc tggagcatgc cggcccgcgc
     2761 aaggccgggg tggtgtcggc cctgtgctgg tgcggtggca tgctggtgtc ggcgctgggg
     2821 gtgtatctgc accagttctg gctgatggtt ctgggttcgg gcgtgattgg tggcattggt
     2881 ttggggctgg gctatatctc ccccgtgtct acgctgatca agtggttccc ggaccggcgc
     2941 ggcatggcca ccggtatggc catcatgggt tttggtggcg gcgccatgat cgggtcgccg
     3001 ctggcggcgg acctgatgaa gtactttgcg acgccgaccg atgtcggggt gatgcagacc
     3061 tttgtggtca tgggcctggt ctatttcgtg ttcatgatgg caggtgcctt tggttaccgt
     3121 gtgcccgaga ccggctggaa gcctgcgggc tggacgcctc ctgcagccga tgctgcgaac
     3181 accatgatca cccagcgcca cgtgcattcc agcaaggtct ggggcattcc gcagttctgg
     3241 ctgatctgga tggtgctgtg catgaacgtg agtgctggca ttggtgtgat cggcatggcc
     3301 tcgcccatgc tgcaggaagt gtttggcggt tcgctgatcg gcgtggccaa gaagtttggt
     3361 gaactggaca aggccgagct gggcgccatc gccgctgtgg ccgctggctt taccgcgctg
     3421 ctgtcgctgt tcaatattgg tggccgcttc ttctgggcca gcctgtcgga caagctgggc
     3481 cgcaagctga cctatgtggt gttttttgta ctcggtgggg ttttgtattt cagcgttccc
     3541 ggcagtgcag cagcgggcag catcgtgctg tttgtggcgg cgttctgcat cattttgagc
     3601 atgtacggtg gtggctttgc cacggtaccg gcctacctgg ctgacctgtt cggcacgcag
     3661 atggtggggg ccatccatgg gcggttgctg accgcctggg cgaccgctgg cgtgctgggc
     3721 ccagtggtgg tgaactacat gcgggaatac cagctggggc tgggcattcc gcgcgagcag
     3781 gtctataacc agaccatgta catcctcacc ggcatgctgg tggtgggcct catttgcaac
     3841 ctgatggttc gccccctggc agacaaatgg ttcatgaccg acgcggagct ggccgaagaa
     3901 aaacggctgg cccacgagag agctgctgcc tccgtggtgg ttcgtggcgc cggggctgga
     3961 gaagatgccg tgagcccggt ggtggtagcc ctggcctggg cggcggttgg cattcccctt
     4021 gcatggggtg tgtaccgcac gctgctgagc gtggccaaat tctttgcgtg attgtttttg
     4081 aactgattag ccatgcgttc cacgtccgaa tcccccgaca tccccaccgt tgcgctgggc
     4141 agcgtggatg acatgcgtga gcgcatccgc aagaaaagca aactcaaggg acgccaggca
     4201 gatgacgctt cgctggcgga agtgcgtgca ttgctgggcc cacggcccga cggtggccac
     4261 cgccgcgacc tactgatcga acacctgcac acgctcaacg acgcgtaccg ttgcctgcat
     4321 gaccggcatc tggtggcgct ggccaaagag atgaacctgc ccatggccga ggtgtacgag
     4381 gtggcgacct tctaccacca cttcgaggtg gtgcagggcg atgccaaggc gccgggattg
     4441 acggtgcggg tgtgtgatgg cttgagctgc gaaatggcgg gtgcacagaa tctgctggcg
     4501 cgcctgaccg acctgttggg cagcgccgat gtgcgggtga ttgcggcccc ttgcctgggg
     4561 cgctgtgagc aggcgccggt ggcggtggtc caccagcgtc cagtgctcca tgccagcccc
     4621 gaagcggtgg tttcaatgat caattcggct gcagcccccg aagaatatgc gcaggacgct
     4681 actaatttca tagcaaattc ctatgcggaa aaaagtgtgt ctccgctgcc tgccgatacg
     4741 gtggccgtgc ctgcctatgc gggctacgac acttaccgcg cccatggcgg atatgcactg
     4801 gtggcggagc tggccagtgg cgcgttgcgt gcagacgctg tccttcagac cctggaagac
     4861 tcggggctgc gcggtctggg tgccgccggt ttcccggcgg gtcgcaaatg gcgcatcgtg
     4921 cgcgaccagc cggctcccaa attgatggcg gtgaatattg acgaggggga gcccggaacc
     4981 ttcaaggacc gcacctacct cgagcgggac ccgcaccgtt tcctggaagg catgctggtg
     5041 gcggcccagg tcgtgggcat agaggccgtc tacatctacc tgcgtgacga ataccacggt
     5101 tgccgtgcca ttctgcaggc ggagctggcc aagcttcagg cgaacccgcc ttgcgagctg
     5161 ccgcacatcg aattgcgccg gggcgccggg gcctatatct gcggcgaaga gtccgccatg
     5221 atcgaaagca tcgaaggcaa gcgcggcgag ccgcgcatgc gtccgcccta catcgcccag
     5281 gtcggcctgt ttggtcggcc cacgttggag cacaatttcg agacgttgta ctgggtgcgc
     5341 gacattgtgg aaaaaggcgc cgcgtggttt gccagctttg gccgcaatgg ccgcaagggg
     5401 ctgcgctcat tcagtgtcag cggccgcgtg cgctaccccg gcgtcaagct ggctcccgcc
     5461 ggcatcacgc tgcgtgaact ggtggacgaa tactgcggcg gcatggccga cgggcatacg
     5521 ctgtacgcct acctgcccgg aggcgccagc ggcggcattt ttccggccca tctggccgac
     5581 ctgccgctgg acttcgacac cctgcagccc tatggctgtt tcatcgggtc ggcggccgtg
     5641 atcgtgctgg gccagcacga ccgcgcacgt gatgcagcgc tgaacaccat gcgtttcttt
     5701 gcccatgaaa gctgcggcca gtgcacgccg tgccgtgtgg gcacggaaaa agccgcacgc
     5761 ctgatggaag ccgaaacctg ggacgccgac accctggacg acctcgcctt tgtcatggcc
     5821 gacgcatcga tctgcggtct cggccaggca gcgcccaacc cgatccgctg catgcacaaa
     5881 ttctttcccc atgaggtcgg tgaggggcct tggcccggcg atctgcccaa gccgcgtggc
     5941 aagggcgcac ggaccgaacg caaggcgccc ttgggcacgc caccggaga
//
LOCUS       DAOSJM010000128         5927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12124_length_5927_cov_4.049898, whole genome shotgun sequence.
ACCESSION   DAOSJM010000128 DAOSJM010000000
VERSION     DAOSJM010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12124_length_5927_cov_4.049898"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..963
                     /gene="phnF"
                     /locus_tag="PKX82_05970"
     CDS             211..963
                     /gene="phnF"
                     /locus_tag="PKX82_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HOF51834.1"
                     /translation="MTSITSSDSHGDRSPKESFWTRIAAELADAIGRGAYPPGERLPS
                     EHALAEQFGVNRHTIRRSLASLSSQGLVRVAQGSGTYVEDFAVDLMLAKRTRHHQSLS
                     QAGLKGGMQVLSTRTERASSAQAAALQVPVRSKLLCLEVLGEAQGQPLHVSERCFPLP
                     RFEGLDDIVKSTGSITAAFAACGVADYTRQESRITAHMPEPAVATHLRQPLNRPVLRV
                     ESVNVDTAGIPIEFATTWFAGDRVKLTVTHDD"
     gene            956..1663
                     /locus_tag="PKX82_05975"
     CDS             956..1663
                     /locus_tag="PKX82_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HOF51835.1"
                     /translation="MTEPTAHRYAVYYAPAPDSPWWAAGRQWLGRCAAQDRPLAQPTV
                     PGLTAAEFSALTAAPRRYGWHATLKAPFALSEGVSLTTLRAALRELGQSLTAFAMPTL
                     RVARLDDFLALVPQGSADRLQAVAAACVTQLNALSAPLSAAELQRRRQAQLTPEQDQM
                     LVRWGYPYVMEHFRFHCSLTGPLNGLSSIQIDAVQGAAESLFHKLPACRLDTLALFAE
                     PTPGADFLLLDHFGLRP"
     gene            1660..2286
                     /gene="phnN"
                     /locus_tag="PKX82_05980"
     CDS             1660..2286
                     /gene="phnN"
                     /locus_tag="PKX82_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism
                     protein/1,5-bisphosphokinase (PRPP-forming) PhnN"
                     /protein_id="HOF51836.1"
                     /translation="MSPQLIYTMGPSGAGKDSLLAWLRQQLSAQAPVHFARRTIDRPV
                     HADGEQHESIPTAEFHAHAARNAFALHWSANSLHYGIRHAELEALQREHWVFVNGSRA
                     YLPTALERFPNLTVLHITASTGVLRQRLLSRGRESPEMVEARVQRAAGFQIPGHCRCV
                     EVHNDASLDAAGHQLLGALSHLDDWSADAWQTPQIAGYWAPTAHTTRP"
     gene            2283..4586
                     /locus_tag="PKX82_05985"
     CDS             2283..4586
                     /locus_tag="PKX82_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF51837.1"
                     /translation="MKDNDPTATSSSKRRAVWVVSLAVVVAVAAAVGWLAPWSTSPQP
                     IAGNTTANPGALHHVGTQVCAQCHTQEAAAWKGSHHELAMQSPSRAAVLAPFRGERAS
                     QNGASTVFSERDGKFLIRTEGPDGKTADFEVAHTLGFTPLQQYLIAMPNGGLQAFNLA
                     WDSRTADQGGQRWFNLQGTQRIAPGDELHWTGRQNNASFMCVECHTTQFKKNFNADLR
                     QYQSTWSESHVACEACHGPGSGHVAWAAKPSLAAAADTSKGLLLQLNERHQVQWTLNA
                     TGNASRSTPPAAQRREMAVCAQCHSHRSQVSDARTPGQPLMDTHIPSLLDKRLFWGDG
                     QMKAEVYNDASFQQSKMYQKGVTCSDCHNPHTLKLRAPGNQVCFQCHAPTKYETPSHH
                     FHPVGSSGSSCASCHMPTTTFMSIDPRHDHSLRVPRPDLSVGSKNPNACTRCHTGKTA
                     QWAAQWASQWYPGLASRHTPLVDALQAADAGDAQALALLFKVITDTDQSAMARASALS
                     RAVPILEPAQLQKVRTLLGDADALVRRTAVESLAGMPPAVRAEWLRPLLQDPIKGVRI
                     AAARWLADAAVTGWDTTDRAHLDAALEEYIAVQRFNADRPESYNNLGTLYADMGKWPQ
                     AEAALQQAIALDPGYAMSSLNLADVYQAQGRESDAQRLIESVIQKHPGNASAHHALGL
                     SLVRQQQKARALAPLRRAAQLEPDNARFAYVLAVLLENSGQVGQAIVTLEKALQANPA
                     DRDAANALRLYCARSANARCPEKYKPN"
     gene            complement(4658..5158)
                     /locus_tag="PKX82_05990"
     CDS             complement(4658..5158)
                     /locus_tag="PKX82_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51838.1"
                     /translation="MARITTLLLVATFSLLTACAGPVPGAGRTDVVLPLSHAWVDGRQ
                     VAYITTDISDAAMARMMGANFVPRLSDAIPKAGQPSVLERVYKFAKDEQISIFQSAPL
                     PVGPANADRSYSPLWRVVMVQWRSPDRVRELKSEEELLAAEDRGDVVLRTTDIVVNCP
                     VVTGAR"
     gene            complement(5202..>5927)
                     /locus_tag="PKX82_05995"
     CDS             complement(5202..>5927)
                     /locus_tag="PKX82_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOF51839.1"
                     /translation="FHAPMLVGADGVWSRLRKEVVDDGAPRVTGHLAFRALVPQADLP
                     EKLRSKVVTAWLGRDFHVVQYPVRRGEWLNVVAIVHGHVYGDPKHWDHSANAAELRTR
                     LAGATKALRDLVHAIPNWRLWALSDRPPMRSASEHARGRIALLGDAAHPMRPYLAQGA
                     GMAIEDADTLARVLDANPDDVPAALQSYANLRWQRNARVQARAIRNGEIFHLRGPMQV
                     GRDLSLRLLGEKLLDVPWLYRGA"
BASE COUNT         1156 a         2040 c         1777 g          954 t
ORIGIN      
        1 ccactgcggc ggctgcgcgc aatgcggcgc cagcaccgac tccagcaagt ccaccggcgc
       61 acgcgatagc agtgctatcc agtccgcgcg gtgcagtgcc ttgcctggcc ccacattgtc
      121 aaatgcgtga aacatcgtgg tgttaccctc aaagttgtat agacaaattt agaatctgtc
      181 cgaactttat ccagcacgta tgtaggtttc atgacaagca tcacttcttc tgattcgcat
      241 ggggatcgca gccccaaaga gagcttctgg acgcgcattg ccgccgaact ggcagacgcc
      301 attggccgcg gcgcctatcc gcccggtgag cggctgcctt cagagcacgc cctggcagag
      361 cagttcgggg tcaaccgcca caccatccgc cgctcgctgg ccagcctctc cagccaggga
      421 ctggtgcgcg tggcccaggg cagcggaacc tatgtggaag atttcgcagt cgacctgatg
      481 ctggccaagc gcacccgcca ccaccagagc ttgtcgcagg ccggactcaa ggggggcatg
      541 caggtgctca gcacgcgcac cgagcgagct tccagcgcgc aagccgccgc cttgcaagtg
      601 ccggtgcgca gcaagctgct gtgcctggaa gttctgggcg aagcgcaggg ccagccgctg
      661 catgtgagcg aacgctgctt ccccctcccc cgctttgagg gtctcgacga catcgtcaaa
      721 agcacaggct ccatcacggc ggcttttgca gcgtgcggcg tggccgacta cacgcgccag
      781 gagagccgca tcaccgcaca catgcccgag cccgccgtgg ccacccacct gcgccagccg
      841 ctgaaccggc cggtcttgcg ggtcgagagt gtcaatgtgg acaccgccgg catccccatt
      901 gaatttgcca ccacctggtt cgctggcgac cgggtcaaac tgacggtaac ccacgatgac
      961 tgaacccacc gctcaccgct acgcggtcta ctacgccccc gcacccgaca gcccctggtg
     1021 ggccgccgga cgccagtggc tggggcgctg cgctgcgcag gaccgaccac tggcccagcc
     1081 cacggtaccg ggactcacgg ccgcagaatt ctccgcactg acggcggcac cccgacgcta
     1141 tggctggcat gccacgctga aagcgccgtt cgcattgtcc gaaggcgttt ctctgaccac
     1201 gctacgcgct gcactgcggg aactgggcca gtcgctcacc gcctttgcca tgcccacctt
     1261 gcgggtagcc cgcctggacg actttctggc cctggtgccg caaggcagtg cagaccgtct
     1321 gcaagccgtg gccgcagcct gcgtgaccca actgaatgcc ctatcggctc cgctgagcgc
     1381 agctgagtta cagcggcgca gacaggcgca gttgactccg gagcaggacc agatgctggt
     1441 gcgctggggc tatccgtatg tgatggagca tttccggttt cattgctcgc tcacgggacc
     1501 gctgaatggt ttgagctcca tacagattga cgcagtgcaa ggagctgccg aatccctgtt
     1561 ccacaagctg ccggcctgcc ggcttgacac cctcgccctg tttgcggaac ccacccccgg
     1621 cgcagacttc ctgctgctgg accactttgg tttgcgacca tgagcccgca actgatttac
     1681 accatgggtc catccggggc cggcaaggac agtctgctgg cctggctgcg acaacagttg
     1741 tctgcacaag cgccggttca cttcgcacgc cgcaccatag accgaccagt gcatgcagac
     1801 ggcgagcagc atgaaagcat cccgacagca gagtttcatg cacacgctgc acgcaatgcc
     1861 tttgccctgc actggtccgc caatagcttg cactatggca tccggcacgc tgagcttgaa
     1921 gcgctgcaac gcgagcactg ggtgtttgtc aacggatcgc gtgcctattt accgactgca
     1981 ctggaacgat ttccgaacct cacggtgctg cacatcaccg cttccaccgg tgtgctgcga
     2041 cagcgcctgc tgtcccgggg tcgggagtcg cccgagatgg tggaggcccg ggtgcaacgc
     2101 gccgccggat ttcagatccc cggccactgc agatgcgttg aagtgcacaa cgatgcgagc
     2161 ctggacgccg ccggacacca gctgctgggc gcattgagcc atctggacga ctggagcgcc
     2221 gacgcctggc agactccgca aattgcggga tactgggctc ccactgcgca cacgacacga
     2281 ccttgaaaga caacgacccc actgccactt ccagttcgaa acgtcgcgcc gtatgggtag
     2341 tctcgttggc ggtagtcgtt gcggtggcgg cagcagtcgg gtggctggca ccctggtcca
     2401 cttcaccaca accgatagct ggcaacacca ccgcaaaccc gggcgcgttg caccatgtcg
     2461 gaacccaggt ctgtgcgcaa tgccatacgc aggaagccgc ggcctggaaa ggctcccacc
     2521 acgaactggc catgcagtcc ccgagccgcg ccgcggtgct tgcgccgttt cgcggggagc
     2581 gcgccagcca gaatggggcg agcaccgtgt tcagcgaacg cgatggcaaa tttctcatcc
     2641 gcaccgaggg tccggacgga aagaccgcag actttgaagt cgcccacacg ctgggtttca
     2701 cgcccctgca gcagtacctg attgccatgc ccaacggcgg cctgcaggcc ttcaacctgg
     2761 catgggacag ccgtaccgca gaccagggcg ggcaacgctg gttcaacctg cagggtacgc
     2821 agcgcattgc accaggcgat gagctgcact ggacaggccg gcagaacaac gccagcttca
     2881 tgtgtgtgga gtgccatacc acgcagttca agaaaaattt caacgccgat ctgcgccagt
     2941 accaaagcac ctggtccgaa tcccatgtgg cgtgcgaggc ctgccatggc ccgggctcgg
     3001 gccatgtggc ctgggcggcc aagccctcac tcgcggcagc tgctgacacc agcaaggggc
     3061 tgctactgca actgaatgaa cgccatcaag tgcagtggac gctcaacgcc accggcaatg
     3121 cctcccgcag cacaccgcca gcagcacagc gccgcgaaat ggccgtctgc gcccagtgcc
     3181 acagccaccg cagccaggtc agcgacgcgc gcacgccggg ccaacccctg atggacaccc
     3241 acatcccctc cctgctggac aagcgcctgt tctggggcga cggccagatg aaagccgagg
     3301 tgtacaacga cgcatccttc cagcaaagca agatgtacca aaagggcgtg acctgcagcg
     3361 actgccacaa cccgcacacg ctcaagctgc gcgcgccggg caaccaggtc tgctttcagt
     3421 gccatgcccc cacgaaatac gaaacaccgt cacaccactt tcacccggta ggctccagcg
     3481 gcagcagttg cgcgtcctgc cacatgccga ccacaacgtt catgtccatc gacccgcggc
     3541 acgaccacag cctgcgggtg ccacgccccg acctgtcggt gggcagcaaa aaccccaacg
     3601 cctgcacccg gtgccacacc ggcaagacag cccaatgggc ggcacagtgg gcatcacagt
     3661 ggtaccccgg actggccagc cgccacaccc cgctggtgga tgccctccag gccgccgatg
     3721 cgggcgatgc ccaggcgctg gccttgctgt tcaaggtgat caccgatacg gaccaatcgg
     3781 ccatggcgcg tgccagtgcc ctctcgcgag ccgtaccgat tctggagcct gcgcagttgc
     3841 agaaggtccg cacgttgctg ggggatgccg atgcactggt gcgcagaact gcggtggaat
     3901 cactggctgg catgcccccc gccgtgcggg ccgagtggtt gcgtcccctg cttcaggacc
     3961 cgatcaaagg cgtgcgcatt gcggccgcgc gctggctggc agatgccgcc gtgacaggct
     4021 gggataccac cgaccgggcc cacctcgacg cggcgctgga ggaatacatc gcggtgcagc
     4081 gcttcaatgc cgaccggccc gagtcctaca acaacctcgg caccctgtat gccgacatgg
     4141 ggaaatggcc ccaggccgaa gccgcgttgc agcaggccat agcgctggac cccggctacg
     4201 ccatgtccag cctgaacctg gcagacgtgt accaggcgca gggacgggaa agcgatgcgc
     4261 agaggctgat cgaatccgtc atccagaagc accccggcaa cgcatccgcc caccacgcgc
     4321 tgggcctgtc cctggtacgg caacaacaga aggcacgcgc actggcaccc ctacgccggg
     4381 ctgcccagct ggagcccgac aacgcccgat ttgcctacgt gcttgccgta ctgctggaga
     4441 acagcgggca agtcggccag gccattgtca cgctggaaaa agcactccag gccaacccgg
     4501 cagaccggga tgccgcgaac gcgctgcgtc tgtattgcgc caggagcgca aacgcccgct
     4561 gcccagagaa atacaagcca aattaacctc tagcccccgt ggatattgcg caagcagcta
     4621 ttatttttat agcaatcaaa caacatttgc gtgagcgcta ccgcgcaccg gtaaccactg
     4681 gacagttgac cacgatgtct gtcgtgcgca ggacaacgtc cccccggtct tctgcagcca
     4741 acagctcttc ttcggacttc aactcgcgca cccggtccgg gctgcgccac tggaccatga
     4801 ccacccgcca cagtgggctg tagctgcggt ctgcattggc ggggccgaca ggcagaggcg
     4861 ccgactggaa aatgctgatc tgctcatctt tggcaaattt gtatacccgc tccagcaccg
     4921 acggctgtcc cgccttcggg atcgcatctg acagccgggg cacaaaattg gcacccatca
     4981 ttcgggccat ggccgcatcg gatatgtcgg tcgtgatgta agccacctgg cgaccgtcga
     5041 cccaggcgtg gctcagcggc aggacaacgt cggtgcggcc tgcgccaggc accggtcccg
     5101 cgcaggccgt cagcagacta aacgtggcaa cgagaaggag cgtggtgatg cgggccataa
     5161 acgtgtcctt aaaaatgggg tttcagtcca accagcgcgt ttcacgcacc ccggtacagc
     5221 cagggtacgt ccagcaattt ctcgcccagc aaccggagcg acagatcccg cccgacctgc
     5281 atcgggccgc gcaggtggaa aatttcacca ttgcgaatcg cacgggcctg cacccgcgcg
     5341 ttgcgctgcc agcgcaggtt ggcgtagctc tgcagggccg caggcacatc gtccggattg
     5401 gcatccagca cccgggccag cgtatcggca tcctcaatgg ccatgcccgc accctgcgcc
     5461 aggtagggtc gcatggggtg cgccgcatca cccagcaagg caatgcgtcc gcgcgcatgc
     5521 tcggacgcgc tgcgcatggg cgggcggtca ctcagggccc acaggcgcca gttggggatg
     5581 gcgtgaacca ggtcccgtag ggctttggtt gcacccgcca gacgcgtacg cagctcagcc
     5641 gcgttggcgc tgtggtccca gtgcttggga tctccataga catggccgtg cacgatggcc
     5701 acgacgttga gccactcgcc ccggcgcacc gggtactgca ccacgtgaaa atcgcggccc
     5761 agccaggcag tgaccacttt ggaacgcaat ttctcgggca agtccgcctg cggtaccaag
     5821 gcgcgaaacg ccagatgccc ggtcacgcgc ggcgcaccgt cgtccaccac ctctttgcgt
     5881 agccggctcc agacgccatc ggcgcccacc agcatcgggg catggaa
//
LOCUS       DAOSJM010000129         5870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12274_length_5870_cov_4.560103, whole genome shotgun sequence.
ACCESSION   DAOSJM010000129 DAOSJM010000000
VERSION     DAOSJM010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12274_length_5870_cov_4.560103"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PKX82_06000"
     CDS             complement(<1..995)
                     /locus_tag="PKX82_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOF51840.1"
                     /translation="MKHRQRPPSVATLVVAACALLTGATAIFPVNSLAQTAPAAAPKR
                     GQPITLNFTNADIEAVARTMATLTGRNVVVDPRVKGTITLNTESPVSPAMAYYQFRTL
                     LRLQGYTVVQGDDLDRVLPEADAKLQSSVVGVSESGEKTASGNQLMTQIIRLQHENAN
                     NLVTILRPLISPNNTINVNPGTNSLVITDYADNLRRLARIVAALDVANATGVEVVQLK
                     HANASDLAPLLLRLLESGGTAAGGQADASFKTTVLAEPRSNALILRAANQARLALAMS
                     LIDKLDQPGGNTANGDAGNIYVVYLKNANAVQLAPMLRAAMGSAVGAAATTTSSAT"
     gene            complement(1013..1810)
                     /gene="gspN"
                     /locus_tag="PKX82_06005"
     CDS             complement(1013..1810)
                     /gene="gspN"
                     /locus_tag="PKX82_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOF51841.1"
                     /translation="MARNRNTPVKAAPWGLAWTGACAGLVLATAVFAPARWLASGLQH
                     LSGDQVRLVDARGSVWQGSAQLVLSGGQGSNDSVALPGSVAWRIRPTWGGLTAQLSAD
                     CCTRMPLLFSAKLAGLQGLHVALADSQSQWPAGLLVGLGTPWNTVQPQGQLLVSSQGF
                     GAQWLEGRLSMAGQVQFDATQMSSRLTTIAPMGSYRLTLQGGSPSSFALATLEGSLQL
                     SGQGQWVGDRLRFEGEASAAPERVEALSNLLNILGRRDGARSIIKLG"
     gene            complement(1814..2323)
                     /gene="gspM"
                     /locus_tag="PKX82_06010"
     CDS             complement(1814..2323)
                     /gene="gspM"
                     /locus_tag="PKX82_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOF51842.1"
                     /translation="MSLTTALAPLQARWTQLAARERTLLALAATVVLGAALWLLVLAP
                     MVSTLRTADTRARALDAQLQQMLGLQAQVQNLQTQPPLRYDEALKALNLATEQTLGKA
                     AQVSVTGDRASVTLQGAPADAVARWLAQARLNACSVPVEARLARVVATKDTPGIAWNG
                     VLIMSLPAR"
     gene            complement(2320..3561)
                     /gene="gspL"
                     /locus_tag="PKX82_06015"
     CDS             complement(2320..3561)
                     /gene="gspL"
                     /locus_tag="PKX82_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HOF51843.1"
                     /translation="MTTLILTLPLTTAAASTPVEGVLSDDGTSVTRVVQTPLALQPAS
                     GAEIVALVPAHKLSWHLLTLPRGTLERGYFGDGGNARLRSVLDGLLEDRLLDETAHMH
                     FALEPGARDGTAVRVAACNRAWLHSWMAALEQASLPVTRIVAEWTPGPDADTLAAMWV
                     TGSPEQPELVCTRFDGVARLPLNATTTALLSATGDNPAAGAVRAEPGVAELAEQALKR
                     PIALQTRAERAVAAAQTGWDLAQFDLLRTRSTRTRKQFSAVTGALLRAPQWRAARWAL
                     LALLLANLVGLQAWAWKEQSSQASKRAAIQSVLTTTFPDVRVIVDAPLQMARAVADLQ
                     RQSGGTSNADLEAMLGQFQAAAPGTPAPSAIEFVAGELVLRGLDSAAPGLADATTRLQ
                     AQGYAARWDGDALMIQQERQP"
     gene            complement(3588..4517)
                     /gene="gspK"
                     /locus_tag="PKX82_06020"
     CDS             complement(3588..4517)
                     /gene="gspK"
                     /locus_tag="PKX82_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HOF51844.1"
                     /translation="MKARQTGAALLAAMLTVTLVASLAAAALWQQWRSIEVESAERAR
                     QQSLWVLTGALDWARLILREDARSGGADHLAEPWAIPLAEARMSTFLSADQSTSVDND
                     NTMLQAFLSGGISDLQARMNVRNLVDDSNKLSEPDLKAFAKLFQQLGLQPAEVTLIAT
                     NLRLALDSGSAAAPDTAPLMPQRTRQLGWLGLSGRSLEALMPHITLLPVRTPLNINTA
                     SPIVMYASVPSMDLAQAQQLASARQVTHFRTLADASKILGEGAGQLNETQHSVNSRFF
                     EVRGRLRLDQTIVEESSVVQRDGIVVKTLWREH"
     gene            complement(4514..5308)
                     /locus_tag="PKX82_06025"
     CDS             complement(4514..5308)
                     /locus_tag="PKX82_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOF51845.1"
                     /translation="MAPTLPHCVWSLPPKGAELAGGGPARRSVAPTLHRCAGRRPQRG
                     FTLVEMLVAIGIMALMAGLSWRGIDGMVQTQTRMQQRADALLTLQAGLGQWAADLDAV
                     VEGPPEQTMDWDSRGLRLLRRNTLSPGDGLLVVAWSRRSIDGQGQWLRWQSLPVRTRK
                     DQQAAWARAALWAQNPGDEEKKLEVRITPLTQWEIFYFRNDAWTNPLSSADAAKAAAT
                     APASPATPPDTQPDGVRLVLTLPEGEAISGVLTRDWVRPPMGGGKS"
     gene            complement(5313..5675)
                     /gene="gspI"
                     /locus_tag="PKX82_06030"
     CDS             complement(5313..5675)
                     /gene="gspI"
                     /locus_tag="PKX82_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HOF51846.1"
                     /translation="MKRTRARGFTLVEVLVALGIVALALAAGVRASASLTRNAERQSD
                     LLLAQLCAENALVTMRLARQMPAVGDNTSTCQQAGRALDVQLAVRPTPNPNFLRVEAL
                     VSEGNSPMLTLSTVVGRY"
     gene            complement(5672..>5870)
                     /locus_tag="PKX82_06035"
     CDS             complement(5672..>5870)
                     /locus_tag="PKX82_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspH"
                     /protein_id="HOF51847.1"
                     /translation="TTLPTQWLGADVQATTSAPLVLGPDPIIGPQQVRLVSISSPARS
                     VTLATDGIRPFSVAPPPGTAP"
BASE COUNT          928 a         1870 c         2078 g          994 t
ORIGIN      
        1 gtggcggaac tggtggtggt agccgcagcc ccgacagcac tgcccatggc ggcgcgcagc
       61 atgggcgcca gctgcactgc attggcgttc ttcaggtaca ccacgtagat gttgcccgcg
      121 tcgccattag cggtattacc accaggctgg tcgagcttgt cgatcagcga catggccagc
      181 gccaggcggg cctggttggc ggcgcgcagg atcagcgcgt tggagcgcgg ctcggccagc
      241 accgtggtct tgaagctggc atcagcctgg ccgccggcag cagtaccgcc cgattcgagc
      301 aggcgcaata gcagcggtgc caggtcagag gcattggcat gcttgagctg caccacttcc
      361 acgccggtgg cattggccac atccagcgcg gcgacgatac gcgccaggcg gcgcaggttg
      421 tcggcgtaat cggtgatcac cagcgaattc gtgccgggat tgacgttgat cgtgttgttc
      481 gggctgatca gtgggcgcag gatggtcacc aggttgttgg cgttttcatg ctgcagccgg
      541 atgatctgcg tcatcagctg gttaccgctg gcggtcttct cacccgattc agagacgccc
      601 actacgctgc tttgcagctt ggcatcggcc tcgggcagca cgcggtccag gtcatcgccc
      661 tgcaccacgg tatagccctg caagcgcagc agcgtgcgga actggtagta ggccatggcc
      721 ggcgacacgg gcgactcggt gttgagcgtg atcgttccct tgacgcgggg gtccaccacc
      781 acgttgcggc cggtcagtgt ggccatggtg cgggcgaccg cttcgatgtc ggcgttggtg
      841 aagttcaggg tgatgggctg gccgcgtttc ggggcagcag caggggcggt ctgggccagg
      901 gaatttacgg gaaatatggc tgtagccccc gtcaataaag cgcaagcagc tacaacaagt
      961 gtagcaaccg atggcggacg ttgacggtgt ttcataccac gcgattccac tgttaaccca
     1021 atttgatgat ggagcgggcg ccatcgcgcc gccccagaat attcaggagg ttggagagtg
     1081 cctccacgcg ctcgggcgcg gcgctggcct ctccctcaaa ccgcaggcga tcaccgaccc
     1141 attggccctg acccgagagc tgcaggctcc cttcgagcgt cgccagtgca aaactggatg
     1201 gggacccccc ttgcagggtc agccggtagc tgcccatggg ggcaatggtg gtcaggcgcg
     1261 atgacatctg tgtggcatcg aactgcacct gtcccgccat ggaaagtcgt ccctccagcc
     1321 attgtgcgcc aaaaccctgg ctggagacaa gcagttgccc ctggggctgc accgtgttcc
     1381 agggggttcc gagcccgacc agcaaccctg cgggccactg ggactggctg tcggccagtg
     1441 ccacatgcag gccctggagc ccagccagtt tggctgaaaa cagcaggggc atgcgcgtgc
     1501 agcagtctgc actgagttgt gcggtgagtc caccccaggt cggccggatg cgccatgcca
     1561 ccgaaccggg cagtgccacc gaatcgttgc tgccctgacc gccggaaagc accagctgag
     1621 ccgagccctg ccagacactg ccacgtgcat caaccaggcg gacctggtcg ccactgaggt
     1681 gttgcagtcc ggatgccagc cagcgtgccg gtgcaaacac cgcggtggcc agcaccagtc
     1741 ctgcgcacgc accggtccac gccagtcccc agggagcggc tttgacagga gtgttccggt
     1801 tgcgggccat ggcttagcgg gcaggcaggg acatgatgag tacgccgttc caggcgatac
     1861 cgggggtgtc tttggtggcg accacgcggg ccagccgcgc ctcgaccggg acggagcagg
     1921 cgttcaggcg ggcctgggcc agccagcggg ccacggcatc ggccggagcg ccctgcaggg
     1981 tcacgctggc acggtccccg gtgacgctga cctgggccgc tttgcccagg gtctgttcgg
     2041 tcgccagatt cagggccttg agggcttcgt cgtagcgcag gggcggctgc gtttgcaggt
     2101 tttgcacctg ggcctgcagg cccagcatct gctgcagctg ggcatccagc gcccgcgcgc
     2161 gggtgtcggc ggtgcgcagg gtggacacca tgggcgccag gaccagcagc cacagcgcgg
     2221 cgcccagcac cacggtagcg gccagtgcca gcagcgtgcg ctcccgcgca gccagctggg
     2281 tccagcgtgc ctgcaggggg gccagtgcgg ttgtcaggct cacggctggc gctcctgttg
     2341 gatcatgagt gcgtcaccgt cccagcgcgc ggcatagccc tgggcctgca gccgggtggt
     2401 ggcgtcggcc aggcccggtg cagcggaatc caggccccgg agcaccagtt cgcctgctac
     2461 aaattcaata gcagatggtg cgggtgtacc gggggctgca gcctgaaatt gccccaacat
     2521 ggcttccagg tcggcgttcg aagtgccgcc gctctggcgt tgcaggtcgg cgacggcacg
     2581 cgccatttgc agcggggcgt cgacgatgac gcggacatcg gggaaggtgg tggtcagcac
     2641 gctctggatg gcggcgcgtt tgctggcctg gctggattgc tccttccagg cccaggcctg
     2701 caggccgacg aggttggcca gcagcagggc aagcagggcc cagcgcgccg cgcgccattg
     2761 gggcgcacgc agcagggcgc ccgtgacggc gctgaattgt ttccgggtac gggtgctgcg
     2821 ggtgcgcagc aggtcaaact gggctaggtc ccagccggtc tgcgctgcag ccacggcgcg
     2881 ctcggcgcgg gtttgcaacg caatggggcg tttgagcgcc tgctccgcca gttccgcgac
     2941 gccaggttct gcccgtaccg cgcccgcagc cgggttgtcg ccggtggcag acagcagggc
     3001 ggtcgtggtg gcgttcaggg ggagccgcgc cacaccgtca aagcgggtgc acaccagttc
     3061 gggttgttcg ggcgagccgg tcacccacat ggctgcaagg gtgtcggcat ccgggccggg
     3121 tgtccattcg gccacgatgc gggtgactgg caggctggcc tgttccaggg cggccatcca
     3181 gctgtgcagc caggcccggt tgcaggcggc gacccgcact gcagtgccgt cgcgtgcgcc
     3241 gggctccagc gcaaagtgca tgtgggcggt ttcgtcgagc agtcggtctt ccagcaggcc
     3301 atcgagcacc gagcgcaggc gggcattgcc gccatcgcca aaatagccgc gttccagcgt
     3361 accgcggggc agcgtcagca ggtgccagga cagcttgtgg gccggtacca gggccacgat
     3421 ttccgccccg gaggcaggct gcagtgccag tggcgtctgc acaacacgtg tgactgaagt
     3481 gccgtcgtcg ctgagcacac cctccaccgg cgtcgaggcc gcggcggtgg tcagtggcag
     3541 ggtgaggatc agcgtggtca tgggtttagt gggggcaact ggcgggttca gtgctcacgc
     3601 cacagggtct tcaccactat tccgtcgcgc tgcaccacgg aactttcttc cacgatggtc
     3661 tggtccagcc gcaggcggcc acgcacttcg aaaaaacggg aattcacgct gtgttgtgtt
     3721 tcgttgagtt gtcctgctcc ctcccccaag atcttgctgg catcggccag cgtccgaaaa
     3781 tgggtgacct gtcgggcgct tgcgagttgc tgcgcctggg ccaaatccat tgaagggacg
     3841 ctagcgtaca tcactatcgg actggcagtg ttgatattta gcggagtgcg gactggaagc
     3901 aacgttatgt gcggcatcag ggcctccaga ctgcggccag acaggcccag ccagcccagt
     3961 tggcgggtgc gttgtggcat caggggcgcg gtgtcggggg ctgcggcact gccggaatcg
     4021 agcgccaggc gcaggttggt ggcgatcagg gtgacttcgg ccggctgcag acccagctgt
     4081 tgaaacagct tggcaaaggc cttgaggtcg ggttcggaca gcttgttgct gtcgtccacc
     4141 aggttgcgca cgttcatgcg cgcctgcaaa tcgctgatgc ccccggacag gaaggcctgc
     4201 agcatggtgt tgtcgttgtc gaccgaggtg ctttggtcgg ccgacagaaa ggtcgacatc
     4261 cgcgcctccg ccagcggaat cgcccagggc tcggccaggt ggtcggcccc cccggagcgg
     4321 gcgtcctccc gcaggatcag ccgggcccag tcgagggcgc cggtcagcac ccacagcgac
     4381 tgctggcgtg cccgttcagc cgactccacc tcgatgctgc gccactgttg ccacagggct
     4441 gctgcggcca agcttgccac cagtgtgacg gtgagcatgg ccgccagcag ggccgcaccg
     4501 gtctggcgcg ccttcatgac ttgcctccgc ccataggggg gcggacccag tcgcgcgtca
     4561 gtaccccgct gatggcttcg ccctcaggca gggtgagcac caggcgcacg ccgtcaggct
     4621 gggtgtccgg gggcgttgca ggggaagcag gagccgtcgc cgccgctttt gcagcatccg
     4681 cgctggagag aggattggtc caggcgtcat tgcggaagta gaagatctcc cactgagtca
     4741 gcggggtgat gcgcacctcc agcttctttt cctcgtcacc cgggttctgg gcccacaggg
     4801 ccgctctggc ccaggctgct tgctggtcct tgcgggtgcg caccggcagg gattgccagc
     4861 gcagccattg gccctggccg tcgatgctgc gccggctcca ggccaccacc agcaggccgt
     4921 cgccgggcga cagggtgttg cgccgaagca ggcgcaggcc gcggctgtcc cagtccatgg
     4981 tctgctccgg tgggccttcg accacggcat ccaggtccgc ggcccactgc cccaggccgg
     5041 cctgcagggt cagcagggca tcggcgcgct gctgcatgcg tgtctgggtc tgcaccatgc
     5101 cgtcgatgcc acgccagctc aggcccgcca tcagagccat gatgccgatg gcgaccagca
     5161 tctcgaccaa tgtgaagccg cgctggggcc ggcgaccggc gcagcggtgg agcgtggggg
     5221 ccaccgaacg ccgcgcaggg ccgcccccag caagttcagc ccccttgggg ggcagcgacc
     5281 acacgcagtg agggagcgtg ggggccatca cttcagtacc gccccaccac ggtggacagg
     5341 gtcagcatgg gcgagttgcc ttcgctgacc agggcttcga cccgcagaaa attggggttg
     5401 ggcgtcggcc gtaccgccag ttgtacgtcc agggcgcgcc ctgcctgctg gcaggtgctg
     5461 gtgttgtcac ccaccgcggg catctggcgg gccaggcgca tcgtaaccag tgcgttttca
     5521 gcacacagtt gggccagcag caggtcggac tggcgctcgg cattgcgggt cagcgaagcc
     5581 gaagcgcgaa ccccggcggc cagcgccagc gccacaatgc ccagcgcgac cagcacctcg
     5641 accagtgtga agccccgggc tcgtgtgcgt ttcatggcgc tgtgcccggt ggtggtgcca
     5701 ctgaaaaagg ccggatgccg tcggtcgcca gtgtcacgct gcgcgcggga ctggaaatcg
     5761 acaccagccg cacctgctgt ggtccgatga tggggtcggg acccagcacc aggggggcgg
     5821 aggtggtggc ctgcacatcg gcacccaacc attgggtggg cagggtggtg
//
LOCUS       DAOSJM010000130         5830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12387_length_5830_cov_4.296277, whole genome shotgun sequence.
ACCESSION   DAOSJM010000130 DAOSJM010000000
VERSION     DAOSJM010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12387_length_5830_cov_4.296277"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1818)
                     /locus_tag="PKX82_06040"
     CDS             complement(115..1818)
                     /locus_tag="PKX82_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51848.1"
                     /translation="MNHPNPAIVSQAAARRLPRIALVLFCMAYVLPGFFGRDAWKSAD
                     MAGLGVMAELLQGTSSWWSPTLMGMSLENPALLPYWLGAWAMRLAPGWMDADFAARIP
                     FGLLLTLTLVATWYGTYYLGRSPKAQPVAFAFGGEARPKDYARSMADGALLALIACLG
                     LAQLSHETTPALAQLGCAALFYYGIGALPYHQWVPSLSIALGLVGMSLSGAPAIAALW
                     GAGAGLIHLLDHSREVDEARPVPRLRLEGMAILLITAIAATTAAVLNLWRWKVELPHA
                     TWADWNGYAQLLVWFTWPAWPLALWTLWRWRHQLFSRNISRHLALPAWLCGVTVAATL
                     TTGSSDRTLLLALPALATLAAFALPTLQRQVAALIDWFTLLFFSGCAFIIWVVWIAMQ
                     TGVPSQPAANVARLAPGFEPGFSLPVFAIALAATVVWAWLVHWRVGRHRAAIWKSLVL
                     PAGGAALCWLLLMTLWMPLLNYAQGYKILVERTTAQLEPVACVETMGLSQGQIAAFQF
                     YGNLQLRPAQTTPTCPWLLAEPGPDMAPPAQLDNSQWNLVVPINHPTDGNQSVLVFKQ
                     R"
     gene            complement(1907..2164)
                     /locus_tag="PKX82_06045"
     CDS             complement(1907..2164)
                     /locus_tag="PKX82_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOF51849.1"
                     /translation="MKEGIHPNYREVCFQDMSNGFKFVTRSCANTKEMIDMEDGRKLP
                     LFKLDTTSESHPFYTGTQKSVDNMGGRVEKFRNRFGKTAAK"
     gene            complement(2236..3501)
                     /gene="rho"
                     /locus_tag="PKX82_06050"
     CDS             complement(2236..3501)
                     /gene="rho"
                     /locus_tag="PKX82_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOF51850.1"
                     /translation="MHLNELKALHVSEVLKQAEELEIENTGRMRKQELMFAIIKKRAR
                     AGEQIIADGVLEILPDGFGFLRSPDTSYTASTDDIYISPSQVRRFNLHTGDMIEGEVR
                     TPKDGERYFALNKLDKVNGGPPEGNKHKVMFENLTPLFPKEQMKLEREGVKTEENITG
                     RVIDIIAPIGKGQRALLVAPPKSGKTVMMQHIAHAISANYPDSYMMVLLVDERPEEVT
                     EMQRTVKGEVIASTFDEPAARHVHVAEMVIERAKRLVELKKDVVILLDSITRLARAYN
                     NVVPSSGKVLTGGVDANALQRPKRFLGAARNVEEGGSLTIIATALIDTGSRMDEVIFE
                     EFKGTGNSEVHLDRRLYEKRIFPSIQLNRSGTRREELLLQPEILQKTRILRQFLYNMD
                     EIESMEMVLKQMRATKTNGEFFEMMRRGG"
     gene            complement(3703..4035)
                     /gene="trxA"
                     /locus_tag="PKX82_06055"
     CDS             complement(3703..4035)
                     /gene="trxA"
                     /locus_tag="PKX82_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HOF51851.1"
                     /translation="MASELIKHVTDATFEADVLQSEQPILVDYWAEWCGPCKMIAPIL
                     DEVSTTYQGKLQIAKMNVDENRDIPAKFGIRGIPTLMLFKGGQLAATKVGAMSKAQLT
                     AFIDQQLA"
     gene            4081..>5830
                     /locus_tag="PKX82_06060"
     CDS             4081..>5830
                     /locus_tag="PKX82_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOF51852.1"
                     /translation="MTETKYPVGTEPSIGDIAKNDASGGLRAFSYGAQHPAVALWVHP
                     QTGLFARLAAEMDRRAAHPARTVVLLPYAQLLPLANRLWLQHGSDGFAPRFETSMNWC
                     TALGGYARQATDITFDMGLDHLTAQAMLAAAGLGAQQDALAGLLVQAAWQLAPLAAAA
                     GPDQRALWADGARQAVLTGVAGNAALQWEVAVSKVAVEWVAISGYPGDVLFAPQVRAG
                     VDRLVVVEGLAPDPMTEGLRRVWGEKLVALPLAQGTPVLLGGGDAGHTFLHACLDAED
                     EAQRSAACALAHIAQGRFPVALVSSDRALTRRVRAMLEDAGVAMRDENGWKLSTSRVA
                     ASVMTLLRAAQWNASSDAVLEWAKVVPAFSGTVDALEAAVRDAAIRDWSAVAIAPAMS
                     QRAELLATCTAIDTVRDRFKGRRTLAAWLQALASALQECALWDAMAGDDVGAKVLACL
                     QLEPANPQEWSRLKEGALWAGARMDLGAFTRWVNQALEGASFQPAYPAEEQVVILPMS
                     QMLGRPFAACVLAGCDEVRLNPGVEPSGGWTAAQRAALGLPFRDALEALMHAIWTQVL
                     QTPVCDVLWRTSDDAGE"
BASE COUNT         1074 a         1641 c         1936 g         1179 t
ORIGIN      
        1 acggagccga gttgggaatt gaaagcgtgc ctggatcagg ctccaccttc agcatcacgt
       61 ttcctgctgt tcgtgtgcgc caggtgcgga cgcttgcacc agcagacaca ttgttcagcg
      121 ttgtttgaag accagcacgg actggttgcc atcggtgggg tggttgatgg gaaccaccag
      181 gttccattgg ctgttgtcca actgggccgg tggtgccata tcgggtcccg gttctgccag
      241 cagccagggg caggtcggtg tggtttgtgc tgggcggagc tggaggttgc catagaactg
      301 gaaggctgcg atctgtccct ggctcagacc catggtttcc acgcaggcaa caggttccag
      361 ctgggcggtc gtccgttcca ccaggatttt gtacccctgc gcgtagttga gcagaggcat
      421 ccacagggtc atgagcagca gccagcagag tgcagcgccg cccgcaggca gcaccaggct
      481 cttccagatg gctgcgcggt ggcgccccac gcgccaatgc accagccagg cccataccac
      541 ggtggccgcc aatgcaatgg cgaatacagg caatgaaaac ccgggttcga accctggtgc
      601 aaggcgcgcc acgttggccg cgggctggct gggtacgccg gtttgcatgg caatccagac
      661 tacccagatg atgaacgcac agcccgagaa aaagagcagg gtgaaccagt caatcagcgc
      721 cgccacctgg cgctggagtg tgggcagagc aaatgccgcc agggtggcca gtgcaggcaa
      781 tgccagcagg agtgttctgt cagacgagcc agtcgtcagc gtggcagcaa ccgtcacacc
      841 gcacagccat gcgggcagtg ccagatgccg gctgatattc cggctgaaaa gctgatggcg
      901 ccaacgccag agggtccaca aggccagtgg ccatgcaggc caggtaaacc agaccagcag
      961 ctgcgcgtag ccattccagt cggcccaggt ggcgtggggc agctccactt tccagcgcca
     1021 cagattcagg actgctgctg tggtcgccgc aatggcagtg atcagcagga tggccatgcc
     1081 ctccaggcga aggcgcggta cggggcgtgc ctcatcaact tcccgggagt ggtccagcag
     1141 atggatgagg cctgcacctg caccccacaa tgcggcgatg gctggtgcac ccgatagcga
     1201 catgcctacc agccccaatg caatagagag gctgggtacc cactggtggt agggcagtgc
     1261 gccaatgccg taataaaaca atgcagcaca accgagctgt gccaaggcgg gggtagtctc
     1321 gtgcgacagc tgtgccaacc ccaggcatgc gatcagggcg agcagggcgc catcggccat
     1381 ggagcgtgca tagtctttgg gccgggcctc tccgccgaac gcaaatgcca ccggttgagc
     1441 cttggggctg cgacccaggt aataggttcc gtaccaggtt gctaccaggg tcagggtcag
     1501 cagcaggcca aatggaatac gtgcagcaaa gtccgcatcc atccacccgg gggccagtcg
     1561 catagcccag gcgcccagcc agtaggggag cagggcgggg ttctccaggg acatacccat
     1621 cagggtgggt gaccaccagg aactagtccc ttgcaggagt tcggccatca cccccaggcc
     1681 cgccatgtcg gcactcttcc aggcgtcccg tccgaagaat ccgggaagca cgtaagccat
     1741 gcagaacagc accagtgcga tgcggggcag gcgtcttgct gctgcctggg agacgatggc
     1801 ggggttggga tggttcactg gacggggctg ggactggtca aaaaaaagca gctcggaact
     1861 ccgggctgct tctaattgcg gactcaggtc aagacctgaa gccggcttat ttggctgcgg
     1921 ttttgccgaa acggttgcgg aatttctcca cacggccgcc catgttgtcc acggacttct
     1981 gggtaccggt gtagaaaggg tgcgattcgc tggtcgtgtc gagcttgaag aggggcagct
     2041 tgcggccgtc ttccatatcg atcatttcct tggtgttggc gcaggaacgt gtcacgaact
     2101 tgaatccgtt ggacatgtcc tggaagcaca cttcgcggta attggggtga atgccttctt
     2161 tcatatcttc tccatcagtt gcgtcagccg cgccagccta tccggcgtac ttttcgcgaa
     2221 accatcgatt atagcctagc caccacggcg catcatttca aagaattcgc cattggtctt
     2281 ggtcgcacgc atttgcttga gcaccatttc catggactcg atttcgtcca tgttgtagag
     2341 gaactggcgc aggatgcggg ttttctgcaa gatttcaggc tgcagcagca actcttcgcg
     2401 gcgggtaccg ctgcggttga gttggatgga cgggaaaatg cgcttttcgt acaggcggcg
     2461 gtccaggtga acttcactgt tgccggtgcc cttgaattct tcaaagatca cttcgtccat
     2521 gcggctgcca gtgtcgatca gggccgtggc aatgatggtc aggctaccgc cttcttccac
     2581 gttgcgggcg gcgcccagga agcgcttggg gcgctgcagg gcgttggcat ccacaccacc
     2641 ggtcaggact ttcccagagg agggcaccac gttgttgtag gcacgggcca ggcgggtaat
     2701 ggagtccagc aggatcacca cgtctttttt caattccacc aggcgcttgg cacgctcgat
     2761 caccatctcg gccacgtgca cgtggcgtgc agcgggttca tcgaaggtgg aggcaatcac
     2821 ctcgcccttg acggtgcgct gcatttcggt cacttcttcg ggacgctcgt ctaccagcag
     2881 caccatcatg tagctgtcag ggtaattggc cgaaatcgca tgggcgatgt gctgcatcat
     2941 cacggtcttg ccgctcttgg gcggggcgac cagcagggcg cgctggccct tgccgatggg
     3001 ggcaatgatg tcgatcacac ggccagtgat gttctcttcg gttttgacgc cttcacgctc
     3061 cagcttcatc tgttccttgg ggaacagggg agtcaagttc tcgaacatga ccttgtgctt
     3121 gttgccttcg ggtggtccgc cattgacctt gtccagcttg ttcaatgcga aatagcgctc
     3181 gccatccttg ggggtgcgta cttcaccttc gatcatgtcg ccagtgtgca ggttgaaccg
     3241 gcgcacctgg ctggggctga tgtaaatgtc gtcggtgctg gcggtgtagc tggtgtcggg
     3301 actacgcaaa aaaccaaagc cgtcgggcag gatttccagt acaccgtcgg caatgatctg
     3361 ttcgcccgca cgtgcgcgct ttttgatgat ggcaaacatc agctcctgct tgcgcatgcg
     3421 gccggtgttt tcgatttcta gttcttcggc ttgcttgagg acttcagaca cgtgcagtgc
     3481 cttgagttcg tttaagtgca tggaatgtgt cccgttgggg gagttgaccg aaatatcgga
     3541 agttggcaaa aaatcagggg gatgggggtg aggcaagacc ggtaactggc ctctgcgttc
     3601 tgaaccgtgg attggaaccg gccttttgct aaggctggtg aacttggcgg attatgacag
     3661 gaaaaagcga ccgtgcggca gcggcacggt cgcaggccga attcaggcca gttgctggtc
     3721 gataaaggcg gtcagctggg ccttgctcat ggcgcccact ttggtagcgg cgagctggcc
     3781 acccttgaac agcatcaggg taggaatgcc acggatacca aacttggcag ggatgtcgcg
     3841 gttttcatcg acattcatct tggcgatctg cagtttgccc tggtaagtgg tggacacttc
     3901 gtccaggatg ggcgcgatca tcttgcaggg gccgcaccac tcagcccagt agtccaccag
     3961 gatcggttgt tcggattgca gtacgtctgc ttcaaaagtg gcatcggtaa cgtgtttgat
     4021 gagctcgctg gccatggtat ttcctttggg agtttgcata agagctaaag ttgcggcatt
     4081 gtgacagaaa ccaagtaccc cgttggcacc gagccttcta tcggcgacat agccaaaaac
     4141 gatgcatctg gcggcctacg ggccttcagc tacggggcgc agcaccctgc cgtagcactc
     4201 tgggtgcatc cccagacagg cctgtttgcc cggctcgcgg cagagatgga ccgacgggca
     4261 gcgcatccgg cgcgcaccgt ggtgttgctt ccttacgcac agcttcttcc cttggccaat
     4321 cgcctctggt tgcagcatgg ctccgatgga ttcgcgccgc gcttcgagac cagcatgaac
     4381 tggtgcacgg cgctgggcgg gtacgcgcgc caggctacgg atataacgtt cgatatgggc
     4441 ctggatcacc tcacagccca ggccatgctg gccgcagcgg gattgggcgc gcagcaggat
     4501 gcgctggccg gtctgctggt gcaggctgcc tggcaactgg cgccgctggc tgcagcagcc
     4561 gggccagacc agcgggcgct gtgggcggat ggggcgcgcc aggcagtgtt gacgggcgtg
     4621 gctggcaatg ccgcattgca gtgggaagtg gctgtctcca aggtggcagt cgaatgggtg
     4681 gccatctccg gatatccggg tgacgtgctg tttgcaccgc aggtccgggc gggggtggat
     4741 cgtctggtgg ttgtggaagg cctggccccc gacccgatga ccgaagggtt gcgtcgcgtc
     4801 tggggcgaga aactggtggc gttgccgctg gctcagggaa ctccagtgtt actcgggggc
     4861 ggtgatgccg gccacacatt cctgcatgcc tgtctggatg cggaggacga agcacagcgc
     4921 agcgccgcct gtgcactggc ccatattgcg caagggcggt ttccggtggc cctggtgtcg
     4981 tcagaccggg cactgacccg acgggtgcgg gccatgttgg aagacgctgg tgtggccatg
     5041 cgggacgaaa acggctggaa actttcgacc agccgggtcg cagccagcgt catgacgctg
     5101 ctgcgcgccg cgcagtggaa tgcgtcctcc gatgccgtgc tcgaatgggc caaggtggtg
     5161 ccagcgttct caggcacggt ggatgcgctg gaggccgctg tgcgggatgc ggcgattcgg
     5221 gattggtccg ccgtcgctat agcgcccgca atgagtcaaa gagcggagct gttggctact
     5281 tgtactgcca tcgataccgt gcgtgaccga ttcaagggac ggcgcacgct ggcagcctgg
     5341 ctgcaggccc tggccagtgc tttgcaggag tgtgcgctct gggatgccat ggccggagat
     5401 gacgtgggcg ccaaggtgct ggcctgcctg cagctggagc ccgcgaaccc gcaggagtgg
     5461 agccgcctga aggagggggc gttgtgggca ggtgcccgca tggatttggg cgcgttcacc
     5521 cgttgggtca accaggctct ggaaggtgcc agtttccagc cggcttatcc ggcggaagaa
     5581 caggtggtca tcctgccgat gagccagatg ctgggacgac ccttcgcggc ctgcgttctg
     5641 gcggggtgcg acgaggtgcg cctcaaccca ggcgtggagc cgtccggtgg ctggaccgcg
     5701 gcgcagcgtg ctgcactggg gttgccgttc cgtgacgcac tagaggcgct gatgcacgcc
     5761 atttggacgc aggtgctgca gacgccagtg tgtgatgtac tgtggcgcac cagcgatgac
     5821 gccggtgaga
//
LOCUS       DAOSJM010000131         5794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12478_length_5794_cov_4.901028, whole genome shotgun sequence.
ACCESSION   DAOSJM010000131 DAOSJM010000000
VERSION     DAOSJM010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12478_length_5794_cov_4.901028"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PKX82_06065"
     CDS             complement(<1..203)
                     /locus_tag="PKX82_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HOF51853.1"
                     /translation="MAAPPPRNFTGDTFFAWDGDVLVVNILGKPAAAKDAIGKPKGTQ
                     LKVSVTAAPKDGKATDHMVRFLAP"
     gene            221..1807
                     /locus_tag="PKX82_06070"
     CDS             221..1807
                     /locus_tag="PKX82_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOF51854.1"
                     /translation="MPPRIILATLNAKYIHASLGLRYLLANMARHGGADLRAMAVLRE
                     FTIRRPVQEVADALLQELGPPTDAVQVVGLGVYIWNVQQTTALVRLLKAQRPQLRVVL
                     GGPEVSHELDTQDVVSLADHVITGWGDVSFPQICKALVHGPQPLMKVIAGVQPPLDEI
                     ALPYDEYDAADLAHRVLYVEASRGCPFKCEFCLSALDKTAWAFNVDQFLAALETLYLR
                     GARNFKFVDRTFNLKIDASVRILQFFLDRMAAQPEAPLQLHFEVVPDHLPERLKAMLA
                     QFPPGVLQLEVGIQSFNPEVQQRIARKQDNATTAENLRWLVEHSHAHLHTDLIFGLPG
                     ETWQSFAQGFDRLHALRPHEIQLGVLKRLRGTPLARRSLPGQPAEFAMVYDAQPPYTV
                     QQTGAVDHEEVKDFLRLARYWDLLANSGRFARSMELLLQGESAFAAFAGFASWLWRTT
                     QDTAGLTPERLVDALFAYLCAQRGLPETVVRDALLADYVGSGARASPAALRGCLPRSA
                     PAPAKQAGGERQMRHRVLPS"
     gene            1879..3858
                     /locus_tag="PKX82_06075"
     CDS             1879..3858
                     /locus_tag="PKX82_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51855.1"
                     /translation="MTLRQTLFLGAGLGILLPALVLAYFQILSRFDFEVDLRVRAPLQ
                     QYADVLARGVAIAIWNVDTAVATELVEAVLRNPDVVQVTVTNEFREVFVRRDAANGLE
                     GSILTETRDVYYNQTPVGHLTIEMSTGRVTRQFLRAQMWLLVALAAQVVISFSLIWLL
                     FDRRLVRPLLRLQDDARRLASGDMTQAVQWDRADEVGALAQGLDTMRVKISGLLAERE
                     RKNLELECELSERRRVEEALGLSQVKFSVIFESSPVAMSVSHNGGDFAVQDVNSAWVR
                     VFGRSRDVAVGAAGEALGMWRDASARMQALSILTQAGDLVRFPAWLVRGDGGQLLCEV
                     SGRTITLGDQSLVVLAYEDITEKHQYEENMLRLNAGLEQRVNERTRELSEALQHLTVA
                     QAELVRTEKMSALGSMVAGIAHELNTPIGNSLTVASTLQDHARSFETHMAAGIKRSKL
                     EEFVGNTKTGASILMRSLQHAADLVSSFKQVAVDQTSLNRRVFHLKESVAEILLTLGP
                     GIRKTTHVVHNAIEADIVMDSYPGPLGQVLTNLVNNAVVHAFEGRAHGNVWVHAEVLR
                     PGWVTIRVRDDGVGIPPENLGRVFDPFFTTRLGQGGSGLGLNIVYNLVHKSLGGTISA
                     ESTLGVGTCFTIVLPLAAESASPEETPPTGPKIGA"
     gene            3855..4691
                     /locus_tag="PKX82_06080"
     CDS             3855..4691
                     /locus_tag="PKX82_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOF51856.1"
                     /translation="MSTPSRYWADWSTRDFARAQANGSLAQTIAVLPVAATEQHGPHL
                     PLQVDTALVNGVVAASLPHIAHDVPALFLPAQAVGLSPEHAAFPGTLTLKAETVIRLW
                     TEIGESVCASGVKKLVLLNSHGGQVGVMDIVARDLRARLGMLVYSVNWFGLPLTGPQG
                     EDVNALFSAHEHRFGIHAGEIETSMMLALAPEQVDMAQAQNFASTSEDRAKRFAILGN
                     GKSAKLGWQMQDYNPAGAVGNAAAATADKGHAVLDAAGRALAALLAEIHQLPDTTLRP
                     SA"
     gene            4720..5151
                     /locus_tag="PKX82_06085"
     CDS             4720..5151
                     /locus_tag="PKX82_06085"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOF51857.1"
                     /translation="MFSFWKKKNRTEAAPIELVPVVERPLAYRRFLRSAFHAKARLNF
                     SGYLREVQLLDVSLKGALVDMGQVLPCPVGARGRLRLSLSTTTFIAMDVSVARVEGSQ
                     LGLKCTHIDLDSVTHLRQLIERNSQDPALLVRELALLTGGA"
     gene            complement(5167..>5794)
                     /locus_tag="PKX82_06090"
     CDS             complement(5167..>5794)
                     /locus_tag="PKX82_06090"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOF51858.1"
                     /translation="GAADYDQLSDLGQRQSQRLGEYFAGKGLQFEAVLTGTLKRHAQT
                     WAGIAKGAGYTHTPLQWAGLDEYNSEAVIHAIHPTPLEKPDTPEMYRHHFRLLRDGLT
                     QWMNGVVSPHGMPTYDTFVHGVNSALEHVRKHHTGNVLIVSSGGPISTAVGHLLGTTP
                     ETTIELNFRIRNSSVTEFTFNPKRSMLVTYNTLPHLDTPDYTDWITFA"
BASE COUNT          958 a         1788 c         1978 g         1070 t
ORIGIN      
        1 ggcgccagaa agcgcaccat gtggtcggtg gccttgccgt ccttgggggc ggcggtcacg
       61 ctgaccttga gctgggtgcc tttgggtttg ccgatggcgt ctttcgctgc cgccggtttg
      121 cccagaatgt tgacgaccag cacgtcgcca tcccaggcaa agaaggtgtc gccggtgaag
      181 ttgcgggggg gtggtgcagc catgctttga taatacgtcc atgcctcccc gcatcatcct
      241 ggccacgctc aacgccaaat acatccacgc ctccctgggc ttgcgctacc tgctggccaa
      301 tatggcgcgc cacggcggcg cggacctgcg cgccatggcg gtgctgcgcg agttcaccat
      361 ccgccgcccg gtgcaggagg tggccgacgc gttgctgcag gaactgggcc cgcctacgga
      421 tgccgtgcag gtcgtggggt tgggtgtgta catctggaat gtgcagcaga ccaccgctct
      481 ggtgcgcctg ctcaaggcgc agcggccgca gctgcgcgtg gtgctgggtg gccccgaagt
      541 cagccatgag ctcgacaccc aggacgtcgt gtctctggcc gaccatgtga ttaccggctg
      601 gggtgatgtg agtttccccc agatctgcaa ggcactggtg cacggcccgc agccgctgat
      661 gaaggtgatt gccggcgtgc agccacctct ggacgaaatc gcgctgccct acgacgaata
      721 cgacgcagcc gacctggcgc accgcgtgct gtacgtcgag gcctcgcgcg ggtgtccgtt
      781 caagtgcgag ttctgcctga gcgcgctcga caagaccgcc tgggcgttca atgtcgacca
      841 gttcctcgcg gcactggaaa ctttgtacct gcgcggtgcg cgcaatttca agtttgtgga
      901 ccgcaccttc aacctgaaga tcgatgcctc ggtgcgcatc ctgcagttct ttctggaccg
      961 catggccgcc cagccggagg cgccgttgca gctgcacttt gaagtcgtgc ctgaccatct
     1021 gcctgagcgc ctgaaggcca tgctggcgca gttcccgccc ggtgtgctgc aactcgaagt
     1081 gggcatccag agcttcaacc ccgaagtgca gcagcgcatt gcgcgcaagc aggacaacgc
     1141 caccacggcg gagaacctgc gctggctggt ggagcacagc catgcccacc tgcacaccga
     1201 cctgattttt ggtctgccgg gggaaacctg gcagagcttt gcgcaggggt tcgaccgcct
     1261 gcacgcgctg cggccgcacg agattcagct gggggtgctc aagcgcctgc gtggcacgcc
     1321 gctggcccgg cgcagcctgc ccgggcagcc ggccgagttc gcgatggtgt acgacgcgca
     1381 accgccgtac acggtgcagc agacgggtgc tgtggaccac gaggaggtca aagactttct
     1441 gcggctggcg cgctactggg atctgctggc caattccggg cgctttgcgc gcagcatgga
     1501 gttgctgctg cagggcgaat cggcctttgc ggcatttgcc gggtttgcca gctggctgtg
     1561 gcgcaccaca caggacacgg cggggctcac gccggagagg ctggtcgacg cgctgtttgc
     1621 ctacctgtgc gcacaacgcg gcctgccgga aacggtggtg cgcgacgcgt tactggcaga
     1681 ctacgtgggc agtggtgcac gtgccagccc ggctgcgctg cgcggctgcc tgccccgcag
     1741 cgcgccggcg cctgccaaac aggcgggtgg cgagcgccag atgcgccacc gggtattgcc
     1801 gtcttgaccc ctggcagggc gggggcgata ctgacttgca tgcaccgtgc gaccccgccc
     1861 caggcatcca gcaggttcat gaccctgcga cagacgctct tcctgggagc cggtctgggc
     1921 attcttctgc ccgcgctggt gctggcctat ttccagatac tgagccgctt tgatttcgag
     1981 gtggacctgc gcgtgcgtgc gccgttgcag cagtatgctg atgtgctggc ccggggcgtg
     2041 gccatcgcca tctggaacgt ggacaccgcc gtggccaccg agctggtgga agccgtgctg
     2101 cgcaatccgg atgtggtgca ggtcaccgtc accaacgaat ttcgcgaagt ctttgtacgc
     2161 cgggatgcgg ccaacggact cgaaggaagc atcctgaccg agacgcgtga tgtctattac
     2221 aaccagacgc cggtggggca cctgaccatt gagatgagta ccgggcgggt gacacggcag
     2281 tttctgcggg cgcagatgtg gctgctggtg gcgctggcgg cacaggtggt gatttccttc
     2341 tcgctgatct ggctgctgtt tgatcggcgc ctggtgcgcc cgctgttgcg attgcaagac
     2401 gatgcccggc gactggccag cggcgatatg acccaagcgg tgcaatggga tcgggcggac
     2461 gaagtgggcg cgctggccca ggggctggac acaatgcggg tcaagatctc cgggctgctg
     2521 gcagagcgcg agcgcaagaa cctggagctg gagtgtgaat tgtccgagcg ccggcgtgtg
     2581 gaggaagccc tgggactgag ccaggtgaag ttttcggtca ttttcgagtc ctcaccggtg
     2641 gcgatgtccg tgtcacacaa cggtggtgat tttgcggtgc aggatgtcaa cagcgcctgg
     2701 gtacgcgtgt ttggccgatc gcgcgatgtt gcagtcggcg ctgccgggga ggcgctgggt
     2761 atgtggcgtg atgcgtccgc acgcatgcag gcgctttcca tcctgacgca agcgggggac
     2821 ttggtgcgtt ttccggcttg gctggtgcgt ggcgatggcg ggcagctgtt gtgcgaggtg
     2881 tcaggcagga cgattacttt gggagaccag tcgctggtgg tgctggccta tgaggacatt
     2941 accgagaagc accagtacga ggaaaacatg ctgcgcctca atgccgggct ggagcagcgc
     3001 gtcaatgaac ggacccgcga actcagcgaa gcgctgcagc atttgaccgt tgcccaggcc
     3061 gagctggtgc gcaccgagaa gatgtcggcg ctgggctcca tggtggcggg cattgcccac
     3121 gaactcaaca cccccattgg caacagcctc accgtagcca gcacactgca ggaccatgcc
     3181 cgaagttttg agacgcacat ggcggcgggc atcaagcgca gcaagctgga ggaatttgtc
     3241 ggtaacacca agaccggggc cagcatcctg atgcgcagcc tgcagcacgc cgccgacctg
     3301 gtgtcgagtt tcaagcaggt ggcggtggac cagaccagcc tgaaccgccg ggtcttccat
     3361 ctcaaggagt cggtggcaga aattctgctc acgctggggc caggcatccg caagaccacg
     3421 cacgtggtgc acaacgccat cgaggccgat atcgtcatgg acagctaccc tggccccttg
     3481 gggcaggtgc tgaccaacct cgtcaacaac gctgtggtgc acgcctttga ggggcgcgcg
     3541 catggcaatg tctgggtgca tgcagaagtc ctgcgcccgg gctgggtcac catccgggtg
     3601 cgcgacgatg gtgtgggtat cccccctgaa aacctggggc gcgtgttcga ccccttcttc
     3661 accacccggc tggggcaggg gggcagcggc ctgggcctga acatcgtcta caacctggtg
     3721 cacaagtccc tggggggcac catctcggca gaaagtacgc tgggtgtggg tacctgcttc
     3781 accatcgtgc ttccgctcgc cgccgagtca gccagcccgg aagagacgcc gccaacaggc
     3841 cctaagatag gcgcatgagc acaccctccc gatactgggc cgactggagc acgcgcgact
     3901 ttgcgcgggc ccaggccaat ggcagcctgg cgcagaccat cgccgtgctg ccggtggcag
     3961 ccaccgaaca acacggtccc catctgccgc tgcaggtcga cacggcgctg gtcaacggcg
     4021 tggtggccgc cagcctgccc catatagcgc acgatgtacc tgcgctgttt ctgcctgcgc
     4081 aggctgtggg cctgagcccg gagcacgccg ccttccccgg cacgctgacg ctcaaggccg
     4141 agaccgtgat ccggctatgg acagagattg gcgagagtgt ttgcgccagt ggtgtcaaaa
     4201 agctggtgct gctgaactcc catggcgggc aggtgggcgt gatggacatc gtggcgcgcg
     4261 acctgcgggc gcggttgggc atgctggtct acagcgtcaa ctggtttggc ctgccgctga
     4321 ccgggccgca aggtgaggac gtcaatgcgc tgttcagcgc gcacgagcac cgctttggca
     4381 tccatgcggg tgagatcgaa acctccatga tgctggcctt ggcgccggag caggtggaca
     4441 tggcgcaggc gcaaaacttt gcctccacct cggaagaccg cgccaagcgt tttgctattt
     4501 tgggcaacgg caagagtgcc aagctgggct ggcagatgca ggactacaac ccggcaggcg
     4561 ccgtcggtaa cgcagctgcg gctactgcgg acaagggcca cgccgtgctg gacgctgcgg
     4621 gcagggcgct ggccgccttg ctggccgaga tccaccagtt gccagacact acactgcgtc
     4681 cgtctgcctg attgcaccgt ctgcaccgtc cattcctcca tgttcagttt ctggaaaaaa
     4741 aagaaccgca ccgaagccgc tccgattgag ctggtgccag tggtcgagcg cccgctggcg
     4801 taccgacggt tcctgcgctc ggctttccat gccaaggcgc ggctcaactt cagtggctac
     4861 ctgcgggagg tgcagttgct ggatgtgtcc ctcaagggcg ccctggtgga catggggcag
     4921 gtattgcctt gcccggtggg cgcgcggggc cggctgcggc tgtcgctgtc cacgaccacc
     4981 ttcatcgcca tggacgtctc cgtggcccgg gttgaaggca gccagctggg cctgaagtgc
     5041 acccacatcg acctggacag cgtgacccac ctgcgccagt tgatcgaacg caactcgcag
     5101 gaccccgccc tgctggtgcg cgagctggcg ctgctcaccg gcggcgcttg accgcaccca
     5161 gggccgtcag gcgaacgtga tccagtcggt gtagtccggc gtgtccaggt gcggcaacgt
     5221 gttgtaggtc accagcatgc tgcgcttggg gttgaaggtg aactcggtca ccgaactgtt
     5281 gcggatgcgg aaattcagct cgatggtggt ctccggtgtg gtgcccagaa ggtgccccac
     5341 cgcggtggaa atcgggccgc cactgctgac gatgagtacg ttgccggtgt ggtgcttgcg
     5401 cacatgctcc agcgcgctgt tgacgccatg cacaaaggta tcgtaggtcg gcatgccgtg
     5461 cgggctgacc acgccgttca tccattgcgt caggccatcg cgcagcaggc ggaaatggtg
     5521 gcggtacatc tccggcgtat cgggcttttc cagcggtgtg gggtggatgg cgtggatcac
     5581 ggcttcgctg ttgtactcgt ccagcccggc ccactgcagc ggcgtgtgtg tgtagcccgc
     5641 gcccttcgca atgccggccc aggtctgggc atggcgcttg agcgtaccgg tcagcaccgc
     5701 ctcgaactgc aggcccttgc cggcaaagta ttcacccagg cgctggctct ggcgctggcc
     5761 caggtcgctg agttggtcgt agtcggcggc gccg
//
LOCUS       DAOSJM010000132         5746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12624_length_5746_cov_4.425409, whole genome shotgun sequence.
ACCESSION   DAOSJM010000132 DAOSJM010000000
VERSION     DAOSJM010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12624_length_5746_cov_4.425409"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..599)
                     /gene="fliJ"
                     /locus_tag="PKX82_06095"
     CDS             complement(114..599)
                     /gene="fliJ"
                     /locus_tag="PKX82_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HOF51859.1"
                     /translation="MAESKSRGIELAIELATRQRDELAKAHAQAIRDVAFGRDQMGNL
                     ESYARDTDAKWLGAAHTAISAGILQHHFQFMERLQQAIAMQTGVIDNLLRQQATAHDA
                     LLQAEFRLAGLNKVLQTRQAAVQLRRQRTEQRHTDEFAALRHLHARAALMHGEHDDHR
                     N"
     gene            complement(592..2004)
                     /gene="fliI"
                     /locus_tag="PKX82_06100"
     CDS             complement(592..2004)
                     /gene="fliI"
                     /locus_tag="PKX82_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOF51860.1"
                     /translation="MTSADISPQVWEDFFARSRNQVLQDSSLEVRGTLTRLAGLVLEA
                     SGIRVPVGSQCIVTSRSRPPVLAEVVGFNNDRAYLMPAGDVHGLSSGAGVAAAAAYVP
                     VPRLGEPQPDLSLADAGMLRLPLGDGLLGRVVDSQGNPMDRMGPILDVASRPLDRKPI
                     NAMDRAPVREVLDTGVRAINALLTVGRGQRIGLFAGSGVGKSVLLGMMARYTQADVIV
                     VGLIGERGREVKEFIEDILGEDGRARSVVVAAPADAPPLLRMQGASYATAIAESFRDQ
                     GKHVLLLMDSLTRYAMAQREIALAIGEPPATKGYPPSCFAKLPQLVERSGNGLHGVGS
                     ITAFYTVLSEGDDQQDPIADAARAILDGHIVLSRTLAESGHYPAIDIEQSASRVMHNV
                     VSREHFELARRFRSVLSKYARGRDLVQIGAYAHGSDPALDEAIVLHDGMDQFLQQDMF
                     EAAPIDSVQDAMEMALQRHG"
     gene            complement(1991..2689)
                     /locus_tag="PKX82_06105"
     CDS             complement(1991..2689)
                     /locus_tag="PKX82_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOF51861.1"
                     /translation="MSRAHSTFIPGEQLGTVTDWSFGAVDQASLRFQAKLKAQAEAEL
                     RSKDEAVRQAGHAEGYAEGFAQGHAQATLEGQRQIADYIATQGEAAANNMAQLFASAQ
                     EQLHESQQVMAKGVLELACELARQVLRHELASNPNALQPVIREAMGMLTSDCKAAVVR
                     MNPVDIEVFAEVLRQEFSSLSMTLLPDSNVTRGGCIVEAAGTVVDGTVEKRWQRAVAS
                     LGLEASWEDANDLR"
     gene            complement(2689..3690)
                     /gene="fliG"
                     /locus_tag="PKX82_06110"
     CDS             complement(2689..3690)
                     /gene="fliG"
                     /locus_tag="PKX82_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOF51862.1"
                     /translation="MANDDGLQDAAILMMSLGEAEASEVFKHLSPKEVQKLGEAIAKT
                     SQITRERVDEVVDRFTGVAASQSLLVSNSGDYVRSVLKLALGDDKAALLIDRILQGGD
                     VSGIESLKWMDPLSVAELLRNEHPQIVAAILVHLEFDQAADVLKHLVERQRNEVMLRV
                     ATMEGIQPTALKDLNEVLFKVLAGGDKIRKASLGGVKTAAEMINLMGTAIEGTVIESI
                     RAHDPDLAQKIMDKMFVFDDVLKLDDKAIQMVLKEVSSDVLIVALKGAQPELKDKILA
                     NMSSRAAATLKEDLESRGPMRLSEVEAQQKEILKTVRRLADEGQIVIGGGGGDDAMV"
     gene            complement(3712..5409)
                     /gene="fliF"
                     /locus_tag="PKX82_06115"
     CDS             complement(3712..5409)
                     /gene="fliF"
                     /locus_tag="PKX82_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOF51863.1"
                     /translation="MSAVAAIPVNPTIAQRLAGLDQGQRLRLALGVVLFVAIGVIGLV
                     MGRQAEWRVLYSNLADKDGGAVIAQLTTLNVPYKYTEGGGAILVPADRVHDVRLKLAS
                     LGLPKGSVNGFEMMESNRFGMTQFQERLTFQRGLEGELTRSIQALSSVSSARVHLALP
                     NQNGFFREQQKPSASVLLSLHPGRTLDKAQLAGIIHLVASSVPEMNASAVSVLDDTGK
                     LLSTPADGSGNGMGVDAEQLQYAQQIEQIYSRRILEILEPIVGRNNVKAQVTAELDFS
                     QTESTTEVHKPNQTPDTGAVRSQQLVESSNGATPAPPAGVPGATTNQPPAQPSAPING
                     QPQALAANGQGAGAAANAASKRESIINYEVDKTIRVVKGATGVIKRINAAVVLNNQST
                     TDDKGKTTSIPLTEAQMEKMTALVRETVGFNKDRGDSVNLMNAAFAPLKSEVSDVPMW
                     RQPEVIDLARSLAWPLGTLALATLVLLGVIRPAMKSLGQPRAAGATDVESRANQIDAL
                     EAEQPARPQLSASSNSNEPAQPTNSELRLEDARKLTRDNPAAVANIVKAWINGEAPA"
BASE COUNT         1022 a         1882 c         1728 g         1114 t
ORIGIN      
        1 gaacggccat gaacccggtg gcaggtgcac ccgcggcgcc agcccttgtc gcagatttgg
       61 acccacgggc atcggcagcc gaaccggctt tggacgcact ctggggggcg gggctagttt
      121 cgatggtcat catgttctcc atgcatcagg gcagcacggg catgcaaatg ccggagtgcc
      181 gcaaattcgt cggtatgacg ttgctcggtg cgctggcgcc gcagttggac tgccgcctgc
      241 cgcgtctgca acaccttgtt caagccggcg aggcgaaact ctgcctgcag cagcgcatca
      301 tgtgccgtgg cttgctgtcg caacaggttg tcgatcaccc ccgtctgcat ggcaatcgcc
      361 tgctgcagcc gctccatgaa ctggaaatgg tgctgaagaa tgccagcaga aatcgcggta
      421 tgggctgcac ccagccattt ggcgtcggtg tcgcgggcgt agctctccag attgcccatc
      481 tggtcccgcc caaacgccac gtcgcggatg gcctgtgcat gggctttggc gagttcatcg
      541 cgctggcggg tcgccagttc aatggccagt tcgatgcccc tgcttttgct ctcagccatg
      601 gcgttgcaga gccatttcca tggcgtcctg tacggaatca atcggggccg cttcaaacat
      661 gtcctgctgc aggaactggt ccatgccgtc atgcagaacg atcgcctcgt ccagcgcggg
      721 gtccgagcca tgggcgtagg cgccaatctg caccagatcg cgcccacgcg catatttgga
      781 aagcaccgac cggaaccggc gcgccaattc gaaatgttcc cgggacacca cgttgtgcat
      841 cacgcgcgag gcggactgtt caatgtcgat ggcggggtag tgaccggact ccgccaaggt
      901 gcgggacagc acgatatgcc cgtccagaat ggcgcgcgcc gcatcggcaa tcgggtcctg
      961 ctggtcatcc ccttccgaga gcaccgtata aaacgccgtg atcgacccca ccccatgcaa
     1021 gccattgccg ctgcgctcca ccagttgtgg cagcttggca aagcacgagg gcggatagcc
     1081 tttggtggct ggcggttcgc caatcgccaa cgcaatttcc cgctgggcca tggcatagcg
     1141 ggtgagcgaa tccatcagca gcagcacatg cttaccctgg tcccggaacg attcggcaat
     1201 ggccgtggcg tacgaggccc cctgcatgcg cagcagcggc ggcgcatctg ccggagcggc
     1261 caccacgacg gagcgtgccc ggccatcctc accgaggatg tcctcaatga actctttcac
     1321 ttcgcggcca cgttcaccga tcagccccac cacaatcaca tcggcctgcg tgtaccgggc
     1381 catcatgccc agcaacacgc tcttgcccac gccagagcca gcaaacagac cgatgcgctg
     1441 cccacgcccc accgtcagca aggcattgat ggcacgcaca ccggtgtcca gcacctcgcg
     1501 cactggcgcg cggtccatgg cgttgatcgg cttgcggtcc agcggacgcg aggctacatc
     1561 cagaatcggc cccatccggt ccatcggatt gccttgtgaa tcgaccacac ggcccagcag
     1621 cccgtcgccc agaggcaggc gcagcatgcc ggcatccgcg agcgataaat cgggctgcgg
     1681 ctcacccagg cgtggcaccg ggacgtacgc cgcggcggcg gcaacaccgg caccgctgga
     1741 aagtccgtgc acatcgccgg caggcatcag ataggcgcgg tcgttgttga aacccaccac
     1801 ctcggccaac accggcgggc gagatcgcga tgtcacgatg cactgcgatc ccaccgggac
     1861 gcggatgcca ctggcttcca gcaccagtcc ggccaggcgg gtgagcgtgc cgcgcacctc
     1921 caggctcgaa tcctgcagca cctggttgcg cgatcgggcg aaaaaatcct cccagacttg
     1981 cgggctgatg tcagcggagg tcattggcat cctcccagct ggcttccagc cccagactgg
     2041 ccaccgcgcg ctgccagcgc ttctccaccg tgccgtccac caccgtaccg gcagcttcca
     2101 caatgcatcc gccgcgggtg acattgctgt ctggcagcaa ggtcatcgac aggctggaaa
     2161 actcctgccg caatacttcg gcaaagactt cgatatcgac cggattcatg cgcaccacgg
     2221 ccgccttgca gtccgaggtc agcatgccca tggcctcgcg aatcaccggc tgcagcgcat
     2281 tcggattgga ggccagttca tggcgtaaca cctggcgggc caactcacag gccagctcca
     2341 gcacgccttt ggccatcacc tgctgggatt cgtgcaactg ctcctgcgcc gaagcaaaca
     2401 gctgtgccat gttgttggct gccgcctcgc cctgcgtggc aatgtagtct gcgatctggc
     2461 gttgtccttc cagggtggcc tgggcatggc cttgcgcaaa cccttcggca tagccttccg
     2521 cgtgtccggc ttgccgaact gcctcgtcct tggagcggag ctcagcctcg gcctgtgcct
     2581 tgagcttggc ctgaaagcgc agcgaagcct ggtctaccgc accaaaactc cagtcggtta
     2641 ccgtgccgag ctgctcaccg ggaatgaagg tggaatgggc gcgggacatc agaccatggc
     2701 gtcgtcgcca cctccgccgc cgatcacaat ctggccttcg tcggccagac gccggaccgt
     2761 cttgagaatt tccttttgtt gcgcttccac ttcggacagg cgcatggggc cacgcgactc
     2821 caggtcttcc ttgagcgtcg ctgcggcgcg ggacgacata ttggccagaa tcttgtcctt
     2881 gagctcgggc tgtgcaccct tgagcgcaac gatcagcaca tcggaagaca cttccttgag
     2941 caccatctgg atggccttgt cgtcgagctt gagcacatcg tcgaacacga acatcttgtc
     3001 catgatcttt tgcgccaggt cggggtcatg ggcgcggatg gactcgatca cggtaccttc
     3061 aatggcagtc cccatcaggt tgatcatctc ggctgcggtc ttcacaccac ccagcgatgc
     3121 cttgcggatc ttgtcgccac cggccagcac cttgaacagc acttcattga ggtccttgag
     3181 tgcggtgggc tggatgccct ccatggtcgc cacgcgcagc atgacttcgt tgcgctggcg
     3241 ctccaccagg tgtttgagca cgtccgcggc ctggtcaaac tccaggtgca ccagaatggc
     3301 ggccacaatc tgtgggtgct cattgcgcag cagctcggcc accgacagtg ggtccatcca
     3361 tttcaggctc tcgatacccg agacgtctcc accctgcaga atgcggtcaa tcagcaaggc
     3421 cgccttgtca tcgcccaatg ccagtttcag caccgaacgc acatagtcac ccgaattcga
     3481 aaccagcaga ctctgggaag cagccacccc ggtgaatcgg tccaccactt catcgacccg
     3541 ctcacgggtg atctgcgatg tcttggcgat cgcctccccg agcttctgca cttccttggg
     3601 ggacaggtgc ttgaatacct ccgaggcctc tgcttcgccc agagacatca tcagtattgc
     3661 agcgtcttgt agtccgtcgt cgtttgccat ttgggtattg tgcgccgcgg gttacgctgg
     3721 cgcttctccg ttgatccacg ctttgacaat gttagcgact gcagccggat tgtcacgggt
     3781 cagttttcgg gcatcttcca gacggagttc ggaattggtg ggctgcgccg gctcgttgga
     3841 gttggagctc gcgctcagtt gcggccgtgc gggctgctct gcctccagcg cgtcaatctg
     3901 gttggcacgg ctttccacgt ctgtggcgcc tgcagcacgg ggttgtccca acgacttcat
     3961 ggccggccgg atcacaccca gcaacaccag ggtggccagc gccagggtcc ccagcggcca
     4021 ggccaggctg cgggccaggt caatcacttc aggctggcgc cacatcggca catcgctcac
     4081 ctcgctcttg agcggggcaa aggcggcgtt catcaggttc accgagtcgc cacggtcctt
     4141 gttgaaaccc acggtttcac gcaccagggc cgtcatcttc tccatttgcg cctcggtcaa
     4201 cgggatgctg gtggtcttgc ccttgtcgtc ggtggtgctc tggttgttga gcacgaccgc
     4261 ggcattgatc cgcttgatca cgccggtagc acccttgacc acgcggatgg tcttgtcgac
     4321 ttcgtagttg atgatggatt cccgcttgga cgctgcattg gctgcagcac ctgcgccctg
     4381 cccgttggcg gccagcgcct ggggctgccc gttgataggt gcactgggct gggcgggcgg
     4441 ctggttggtc gtcgcaccgg gcacaccggc agggggtgca ggggttgcgc cattggagct
     4501 ttccaccagc tgctggctgc gcacagcgcc ggtgtcgggc gtctggttcg gcttgtgtac
     4561 ttcggtggtg gactcggttt gcgagaagtc cagctcggca gtgacctggg ccttgacgtt
     4621 gttgcgcccc acgatcggct ccagaatctc cagaatgcga cggctgtaga tctgctcgat
     4681 ttgctgggcg tattgcagtt gctcggcgtc cacacccatg ccattgcccg aaccatcggc
     4741 cggcgtggac agcagtttgc cggtgtcatc gagcacgctc acggccgacg cattcatctc
     4801 cggcacactg gacgccacca gatggatgat gcccgccagc tgtgccttgt ccagggtacg
     4861 acccggatgc aggctcagca ggacggaagc cgacggcttc tgctgctcgc ggaaaaagcc
     4921 gttctggttg ggcagcgcca ggtgcacccg ggcactggac accgacgaca acgcctggat
     4981 cgaccgcgtc aactcgcctt cgagcccacg ctggaacgtc agccgctcct ggaactgggt
     5041 catgccgaac cggttggact ccatcatctc gaacccgttg accgaacctt tgggcaagcc
     5101 cagtgaggcg agcttcaggc gcacgtcgtg cacacggtcg gcgggaacca ggatggcacc
     5161 gcccccctcg gtgtatttgt agggcacgtt cagcgtcgtc aactgggcaa tgaccgcacc
     5221 gccgtccttg tccgccagat tggagtacag aacgcgccat tcggcctggc gacccatgac
     5281 caggccaatg accccaatgg ccacgaacag cacgacaccg agcgccagtc gcaggcgctg
     5341 gccttggtcc agcccggcca ggcgctgggc gatggtggga ttgacaggaa ttgcggcaac
     5401 tgcagacatc ttggcttccg gtgctgtggg gcgcaggtcg cctcacgaat gggtctgatt
     5461 attcgacccg gcttcgaaaa cattaggttg aaaagggctg gatttaccct ccatttgaaa
     5521 ataatggcca caccgcgtcg cccctagtat tacccccaat caagttttac tgggagcccg
     5581 caatggacct caaactcaac cctgtctcaa tgccgaccac catccgcccc ggtgcgggcg
     5641 tgcgccccgg cagtgcaacg ggcttacagg aggcgggcgg aggcttctcg ggagcattga
     5701 agagcgccct caattccgtg agcgctgcgc aaaacgaatc cacccg
//
LOCUS       DAOSJM010000133         5736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12647_length_5736_cov_3.810069, whole genome shotgun sequence.
ACCESSION   DAOSJM010000133 DAOSJM010000000
VERSION     DAOSJM010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12647_length_5736_cov_3.810069"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1083)
                     /locus_tag="PKX82_06120"
     CDS             complement(<1..1083)
                     /locus_tag="PKX82_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006404545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF51864.1"
                     /translation="MDFAHSPRAQDLRQRLDAFLQRYVLPYNAAWHRSVQEGVYPPPF
                     LEDLKMLAREEGLWNLFLPTLRDDQPGTRLSNLDYAPLAESMGRLPWAAEVFNCSAPD
                     TGNMELLHRFATPAQNTQWLMPLLGGEIRSAFAMSEPDVASSDPTNLQTTVRRDGDQL
                     VLHGRKWFITGAAHPQCRLLIVMCRNDGEEGGSPHSHHSMVLVPLDTPGVQVLRNIAV
                     VHHHAPEGHCEIVFKDVRLPADHLLGLWGEGFAMAQARLGPGRVHHCMRTIGQCELAL
                     ELATERILERHAFGKYLSDFSNVQDWVAESRIEIDQARLLVLQVAWALDQGALEPAQL
                     RSQVAAIKVVAARLQTRVVERAMQAFG"
     gene            1209..1910
                     /locus_tag="PKX82_06125"
     CDS             1209..1910
                     /locus_tag="PKX82_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOF51865.1"
                     /translation="MTSIKLCDIAANVRAKRERRKESRPGELLAAALELFVEKGFAAT
                     RAEEVAKRAGVSKGTLFLYFSSKEELFKAVVRENISGRFSEWTAELENYQGSSADLLR
                     YCLTVWWERVGSTKASAIPKLMMSEGSNFPELAAFYELEVIRPGDELVRRIIQRGVSR
                     GEFRPIDMKYAAYLVLAPMLFLTVAMHSSGPWADPSANLDPQEYIAMQVNILLHGLCV
                     DAPPSNTHPTKNETP"
     gene            1907..3037
                     /locus_tag="PKX82_06130"
     CDS             1907..3037
                     /locus_tag="PKX82_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOF51866.1"
                     /translation="MKRWLKWIVIAVVVALLALGVLRALSARKATKEALESQQAAQKA
                     QPTIALTATDSVQAKTMELSQGLAISGSLKAASSAFVKARVAGELQGLTVREGDFVKV
                     GQVIAHVDPTESRARARQAQQQAESARAQVDIAKRSFDNNRSLVEQGFISKTALDSSI
                     ATLSSAEANYRAAQAGAEVATKALEDTVLRAPISGQIAQRLAQTGERVAIDARIVEIV
                     DLSRLELEASLSAADSLSVKVGQTAELTIEGAAKPVAAKVVRVNPTAVAGSRAVLAYL
                     ALESNSGLRQGLFAQGTLATGNARVLALPLNAVRTDKPQPYVQLLTNGQVSHTTVTLG
                     ARGAADGHTMVEVAGIPENAEVLSGTVGPVREGTLVKRQGAK"
     gene            3038..>5736
                     /locus_tag="PKX82_06135"
     CDS             3038..>5736
                     /locus_tag="PKX82_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOF51867.1"
                     /translation="MWFTRVSLKNPVFATMVMVALVVLGLFSLQRLQVDQFPNIDFPV
                     VVVIADYPGASPEIVESEVSKKIEEGVNSIAGINALTSRSYEGQSVVVIEFGLHIDGR
                     KAADDVREKVAAVKPSLRTEVKEPRVLRFDPAARAIWSVAVLPDTKVQGAKVPSAVEL
                     TNWAEQTFKRRLENVRGVGSVTVVGGTKREINIYLNPQSLEAFGITPDQVVTAVRNEN
                     QDLPVGTIRSSEQERVIQIEARMERPEDFGRIIVARKNGAPVRLDQVARVADGAQEVD
                     SLALYNGQRTLLLNVQKSQDENTIAVVDGLIKTLAEVQKQLPPGVRLEQITDGSRQIR
                     VSVENVRRTLIEGAVLTVLIVFLFLNSWRSTVITGLTLPIALIGTFLFMNLFGFTINM
                     ITLMALSLCVGLLIDDAIVVRENIVRHVQMGKNAYDASMDGTQEIGLAVLATTLSIVA
                     VFMPIGFMGGIIGKFFHEFGITIVAAVMISMFVSFTLDPMLSSIWHDPSIEAHGHRHA
                     PVTLYDKTIGRVTGWFDQATDWLADAYQSILRWALVHKLKTVALAVAIFVTSIFMVPL
                     LGTEFVPKSDFSETTLSFYTPVGSSLEVTEAKARQVEAIIREFPEVRYTLSTINTGNA
                     QGKMYASIYIRLVDRKLRSFSVDQMSVTLRQRLAQVPGITVTHVGLLDSVGGQKQVLF
                     SIQGPDTAELERLTRLATDRVRTIPGLVDLDSTVKPNKPTLDVQVRRDAASDLGLGVA
                     QIGSALRTLVAGQTVGNWRAPDDQTYDVNVRLAPQARNTPQDLDQLPFALGTNADGSQ
                     RIVRLNQIATVKESTGPNQINRRDLTREVAISANVSGRSAGEVSADITKIMNDIAMPP
                     GYSYKFSGSTKDMAEAFGYAISALVLAIVFIYMILASQFKSFL"
BASE COUNT         1153 a         1830 c         1722 g         1031 t
ORIGIN      
        1 cccaaaggcc tgcatggccc gttcgacaac ccgtgtctgc agccgcgcag ccacgacctt
       61 gatggccgcc acctgagaac gcaattgcgc aggctccagc gccccctggt ccaatgccca
      121 ggccacctgc agcaccagca accgggcctg gtcgatctcg atccgtgatt cggccaccca
      181 gtcctgcacg ttggaaaaat cggacaaata cttcccgaag gcatggcgct ccagaatgcg
      241 ctcggtggcc agttccagcg ccagttcgca ctggccaatg gtccgcatgc aatggtgtac
      301 ccgacccggg cccaggcgcg cctgggccat ggcaaacccc tcaccccaca agcccagcaa
      361 gtggtccgcg ggcagccgta catccttgaa cacgatctcg caatgccctt ccggggcatg
      421 gtggtgcacg accgcaatgt tgcgcagcac ctgcacaccc ggcgtatcca gaggcaccag
      481 caccatgctg tggtggctgt gcggcgagcc cccctcctcg ccgtcgttgc ggcacatgac
      541 gatcagcaac cggcactggg ggtgggccgc gccagtgata aaccatttgc gcccatgcaa
      601 caccagctgg tctccatccc ggcgcaccgt ggtttgcagg ttggtggggt cactggaggc
      661 cacatccggc tcggacatgg caaacgccga acggatctcg ccgcccagca gcggcatgag
      721 ccattgcgtg ttctgggcgg gcgtggcaaa gcggtgcagc agctccatat tgccggtatc
      781 gggagcactg cagttgaaga cttccgcagc ccacggcagc cgccccatgg attcagccaa
      841 cggggcgtag tcgaggttgg acagccgtgt tccaggctgg tcgtcccgca atgtcggcaa
      901 gaaaaggttc cacagccctt cttcgcgcgc cagcatcttg aggtcttcca ggaatggcgg
      961 ggggtagacg ccctcctgca ccgagcgatg ccaggctgcg ttgtaaggca gtacatagcg
     1021 ctggagaaag gcatccagac gctggcgcag atcctgcgcc cggggggagt gtgcgaagtc
     1081 catggtgcat tgtcctgccc aaaggctgcg gcagagggaa cactggggac atgcaccgct
     1141 ccgtgcgcca gcgccttgac cacttccacg caaacctcta cactaataac caatcagtca
     1201 gtaatttaat gacctccatc aaactctgtg acatcgccgc caatgtgcgt gccaagcgcg
     1261 aacgccgcaa ggaatcccgc ccgggtgaac tgctggccgc cgcgctggag ctgtttgtgg
     1321 agaaagggtt tgcagccacc cgtgccgaag aagttgccaa acgggcgggg gtgtccaaag
     1381 gcacactgtt cctttacttc tccagcaagg aagagctgtt caaggcggtg gtacgcgaaa
     1441 acatctctgg gcgcttttcc gaatggacgg ctgagctgga aaattaccag ggcagcagtg
     1501 ccgaccttct gcgctactgc ctgacggtct ggtgggagcg cgtgggctcc accaaagcct
     1561 ctgccattcc caagctgatg atgagcgaag gcagcaattt cccggaactt gcagcttttt
     1621 atgaactgga agtcattcga cctggtgatg agctggttcg gcgcatcatc cagcgcgggg
     1681 tgtcccgcgg ggagttccgg ccgatcgata tgaagtacgc cgcttacctg gttctggcgc
     1741 ccatgctgtt cctgaccgtg gcgatgcatt cgtcaggccc atgggcagac ccctccgcca
     1801 acctcgaccc gcaggaatac attgccatgc aggtgaatat cctgctgcac ggcctgtgcg
     1861 tcgatgcccc accttccaat acccacccta ccaagaacga gacaccatga aacgctggct
     1921 gaaatggatt gtgattgcag tggttgtcgc cctgctggcc ctcggcgtgt tgcgcgccct
     1981 gtctgcacgc aaagccacca aggaggcgct ggaatcccag caggccgccc aaaaagccca
     2041 gcccaccatc gcattgaccg cgacagatag tgtgcaggcc aaaaccatgg aactctcaca
     2101 ggggctggcc atttcgggat ccctcaaggc agcttcttcc gcctttgtca aagcccgcgt
     2161 ggcaggcgag ctgcaaggtc tgaccgtacg tgaaggcgac ttcgtcaagg tgggccaggt
     2221 catcgcccat gtagatccca cagaatccag agcgcgcgcg cgccaggcac aacagcaggc
     2281 cgaaagcgcc cgcgcccagg tcgatattgc gaagcgcagt tttgacaaca accggtcgct
     2341 ggtcgagcag gggttcattt ccaagaccgc gctggactcg tcgatcgcca cactgtcgtc
     2401 cgcagaagcc aactaccgtg cagcccaggc gggcgcggaa gtcgctacca aggcgctgga
     2461 agacaccgtc ctgcgcgcac cgatctcggg acagattgca caacgcctgg cacaaaccgg
     2521 agagcgcgtg gcgatcgacg cccgcatcgt tgaaatcgtg gacctgagcc gtctggaact
     2581 ggaagcgagt ctcagtgcgg ccgactctct gtccgtcaag gtcgggcaaa ccgcagagct
     2641 cacaattgaa ggcgcagcca aacccgttgc agccaaagtg gttcgcgtca atccgaccgc
     2701 tgtggccggg agccgtgcgg tgctcgccta cctggcactg gagagcaaca gtggtctgcg
     2761 ccagggcctg tttgcccaag gcacgctggc caccggcaat gcacgcgtgc tggcactgcc
     2821 actcaacgca gtgcggaccg acaaacccca accctacgta cagctgttaa ccaatggcca
     2881 ggtcagccac acgaccgtga ctttgggtgc gcgcggcgcg gcggacggac acaccatggt
     2941 cgaagtggcc ggcatcccgg aaaacgccga agtgctcagt ggcaccgtgg ggccggtccg
     3001 cgaaggaaca ctggtcaaac gccagggtgc caagtaaatg tggttcaccc gcgtatccct
     3061 caaaaatccg gtctttgcca ccatggtcat ggtggcgctg gtggtgctgg gcctgttttc
     3121 cctgcagcgc ctgcaggtgg accagttccc caacatcgat ttccccgtcg tggtggtgat
     3181 tgccgactac cccggcgcgt ccccggagat tgtcgaaagt gaagtctcca aaaagatcga
     3241 ggaaggcgtc aattcgattg caggcatcaa tgcactgaca tcgcgcagct acgaaggcca
     3301 gagcgtcgtg gtgattgaat ttggcctgca tatcgatggc cgcaaggctg ccgatgatgt
     3361 gcgcgagaaa gtggctgctg tcaaacccag cctgcgcacc gaggtcaagg agccacgcgt
     3421 gctgcgcttc gatccggcgg cacgcgccat ctggtcggtc gcggtattgc cggacaccaa
     3481 agtgcagggt gccaaggtac ccagtgccgt ggaattgacc aactgggcgg agcagacttt
     3541 caagcggcgc ctggagaacg tgcgcggtgt gggctccgtc acggtcgtgg gtggtaccaa
     3601 acgggaaatc aacatctacc tcaaccccca gtcactggag gcgtttggca tcacccccga
     3661 tcaggtggtt acggcagtac gcaatgaaaa ccaggacctg ccggtgggca ccatccgctc
     3721 cagcgagcag gagcgcgtga tccagatcga ggcgcgcatg gaacgtccgg aggactttgg
     3781 ccgcatcatc gtggcgcgca agaatggcgc cccggtgcgg ttggaccagg tggcgcgtgt
     3841 cgccgacggc gcacaggagg tcgacagtct ggcgctctac aacggccagc gcaccttgct
     3901 gctcaatgtc cagaaatcgc aggacgaaaa caccattgcg gtcgtggatg gcctgatcaa
     3961 gaccctcgct gaagtgcaga agcagttgcc cccgggcgtc cggctggagc agattaccga
     4021 cggctcacgg caaatccggg tctcggtgga gaacgttcgc cgcaccctga ttgaaggtgc
     4081 cgtgctcacc gtgctgatcg tgttcctgtt cctcaactca tggcgctcca ccgtcattac
     4141 cggcttgacg ctgcccattg ccctgatcgg caccttcctg ttcatgaacc tgttcgggtt
     4201 caccatcaac atgatcacgc tgatggcact ctccctgtgc gtgggcttgc tgattgacga
     4261 tgccatcgtg gtgcgggaaa acatcgtgcg gcacgtgcag atgggcaaaa acgcctacga
     4321 cgcctccatg gacggcaccc aggaaattgg cctggcggtg ttggccacca cgctgtcgat
     4381 cgtggcggtg ttcatgccca ttggattcat gggcggcatc atcggcaagt tcttccacga
     4441 gttcggcatc accatcgtgg ctgcggtgat gatttccatg ttcgtgagtt tcacgctcga
     4501 ccccatgctg tcgagcatct ggcacgaccc cagcatcgaa gcccatgggc accggcacgc
     4561 gccggtcacc ctgtacgaca aaacgattgg ccgggtgacg ggctggtttg accaggccac
     4621 cgactggctg gccgatgcct accagagcat cctgcgctgg gctctggtac acaagctcaa
     4681 gaccgtggcg ctggccgtgg ccatctttgt gacaagcatc ttcatggtgc cgctgctcgg
     4741 aaccgagttt gttcccaagt cagacttctc cgagactacg ctgagcttct ataccccggt
     4801 aggttcttcg ctggaggtca cggaggccaa ggcccgccag gtcgaagcca tcatccgcga
     4861 gttccccgaa gtgcgctaca cactctcgac catcaacacg ggcaacgccc agggcaagat
     4921 gtacgccagc atctacatcc gcctggtgga ccgcaaattg cgcagcttca gtgtcgacca
     4981 gatgtcggtc accctgcgcc agcgactggc ccaggtgcct ggcatcactg tcacccacgt
     5041 cggtctgctg gattccgtgg gcggccagaa gcaggtcctg ttctccatcc aggggccgga
     5101 caccgcggag ctggagcgac tgacccgcct ggccaccgac cgggtgcgca ccattcctgg
     5161 cctggtggat ctggactcca ccgtcaaacc caacaaaccc acactcgatg tgcaggtccg
     5221 gcgcgacgcc gcttcggacc tgggattggg cgtggcccag attggcagcg ccctgcgcac
     5281 gcttgtggcc ggacagacgg taggcaactg gcgcgcaccc gatgaccaga cctatgacgt
     5341 caatgtgcgg ctcgcaccgc aagcccgcaa tacgccccag gatctggatc agctgccttt
     5401 cgccctgggc accaatgccg acggcagcca gcgcattgtg cggctcaacc agattgccac
     5461 ggtgaaggaa agtaccggcc ccaaccagat caaccggcgc gacctgaccc gtgaggtggc
     5521 gatcagcgcc aacgtgtcgg gccgctcagc cggcgaggtc tctgccgaca tcaccaagat
     5581 catgaacgac attgccatgc cgcctggcta cagctacaaa ttcagtggct ccaccaagga
     5641 catggcggag gcctttggct acgccatctc ggcgctggtg ctggccatcg tgttcatcta
     5701 catgattctg gccagccagt tcaagagctt cctgca
//
LOCUS       DAOSJM010000134         5721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12686_length_5721_cov_4.331274, whole genome shotgun sequence.
ACCESSION   DAOSJM010000134 DAOSJM010000000
VERSION     DAOSJM010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12686_length_5721_cov_4.331274"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PKX82_06140"
     CDS             <1..431
                     /locus_tag="PKX82_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOF51868.1"
                     /translation="LRARFVEDGDPWVAPEEAKSSNFVDIAEAPVAQTAAVSEQDTPA
                     APLAVNLHDALNELPLGSWIELKSNGQWVRTQLTWFSPHGTLFLFTNSVGATQSMTRR
                     SCDRLIAAGHLRLISGQPVVEEALDAVAKAAMRNSLDSTH"
     gene            638..>5721
                     /locus_tag="PKX82_06145"
     CDS             638..>5721
                     /locus_tag="PKX82_06145"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOF51869.1"
                     /translation="MRIATHLAALGALMAQTAHARDGALASGWLADGWEIVWNMALPP
                     LCMAVVILIWQGYTRIRHGRSADVFQPENRGPLLWALLAFSAGMAGFAYQAQTTDKAV
                     HDDAWKRFQANVDRVEADVLGKFEGLLPPLRGARGALVSSEYLSRSEFRKFMQSRDID
                     QEFPGVRGLGIIDRIPRTGLNAFVAAQRKDGAPDFTVKTSGTASDLFIIKYVEPVERN
                     RQAVGLDVGAEPVRRAAAERAVRQGKPALTGRITLVQDDQKRAGFLYFLPYYGSVRVP
                     ETEAERIRQLRGWTYAPIVLAELMVGTQRSLQGQTEFQLFEGAEVAASKLLFDSTLPL
                     GATEVPDGVDHVRKHMFHTVRPLLIGGQLLTLRASASDDFASTLDIQAPARMALYGSV
                     CSVLAGALVWLLFSARVRVVATAGRMTAELGRLALVAKGTSNAVVFTDVRGLTVWVNE
                     GFTRITGYTSDEVMGKAPGYLLQNHNTDPAVVRAIGDAVRARKGGRFEILNQHKDGQE
                     YWVSLEIQPVCDARGEVNGFMAIETDITAEKGAKEALVREKERFDRILSGTNVGTWQS
                     NLQTGESQCNARWAGMMGFEPHEVVPNADVFWTQRVHPEDLVRTTNATQRCRQGLADE
                     YSTEVRVKRKDDSWMWILSRAKVMSRTAEGDVEWIGGIHTDISDTKALEIGLRDMESF
                     LHRAGRLAGVGAWEVDLKTGEVKWSEQTCLIHGVEPDFKPTVEEALSFYTEQGRIEMQ
                     AAIQRSIDMALGWDVTTQILTAQGQLRWIRVAGEPEFDDSGAVRLVGAFQDVTEQRET
                     QLRIERSEAILRASIDAVSDAYVLYDPQDRLAYCNEKYRELYAASSDLIVPGATFESI
                     IRGGAERGQYVEANGRVDAWVAERMRVHLEGNTNMEQRLDNGRWLRVVERRMPDGHRV
                     GFRVDITELKLANEATQAANATLARTSATLQAVLDSAVEVGIMATDLDKRVTVFNRGA
                     ENLTGYAAGDMLGSSGLGRLFERSQMDLVRESLSLQLGRQPNDDEVFLEVAKCADSPE
                     WTLVRKDGTMFTSSMVISSMRDAGGALSGYMAIVYDVTRQKEYEASLRDAMRRAEESS
                     VAKSQFLANMSHEIRTPMNAILGMLQLLHSTRLSPQQLDYADKTEGAARSLLGLLNDI
                     LDFSKVEAGKMQLELEPFALSRLLDELSTILSSNLGSKNVDLLFDVDPDIPATLVGDA
                     LRIKQVLINLGGNAVKFTEQGHVLIGLRMLARHTDRVRLEISVVDSGIGIAPENQARI
                     FNAFTQAESNTTRRFGGTGLGLVISKRLIRLMGGELELSSALGEGSTFRFALELPVPP
                     DAVAFGERARALSATDVVQTLLVDDNPVALEVNAAAMRSLGWSVVQAASGEQAVSLVT
                     QRQAAQMPAFDAVFVDWHMPGMDGWETLRMIRRVVGSGPGMALVLMSGQSRAALFERT
                     EREQALLNGYMVKPLTADMFRDALAQAYDAPYVESTQTNLGSAGDAVDVTPLKPLGGM
                     RILVVEDNPINQQVASELLRAQGASVTLANNGQEGVDAVLTANPPFDVVLMDLQMPVM
                     DGLTAARTIRSHARWGGLPIIAMTANAMASDREACLEAGMNDHVAKPFDLNDLVKTLV
                     DHTHWTPRGEVPVGTPAEVPPAGGNRNLLGQIWPSGVDVEAALNRMGGNAGLLQRSIA
                     AFLRYAEVLPERVRGWLDSHSLADVRR"
BASE COUNT         1108 a         1563 c         1928 g         1122 t
ORIGIN      
        1 gactgcgtgc ccgatttgtg gaggatggcg acccgtgggt cgcacccgag gaggcaaagt
       61 cttccaactt tgtcgatatt gcggaagcac cggtggcaca gaccgctgcg gtgtcggaac
      121 aagacacccc tgcggcacct ctggcagtca atctccacga cgcactcaac gaactgccgc
      181 tgggctcctg gatcgagctg aaatcgaacg gacagtgggt gcgcacgcag ctcacctggt
      241 tcagtccgca tggcacgctg ttcctgttca ccaacagcgt gggagcgacg cagtccatga
      301 cacgacggtc ctgcgacagg ctaatcgctg cgggccatct gcgcctgatt tccgggcaac
      361 ctgtggtgga agaggcgctg gacgctgtag ccaaagcggc catgcgcaac agcctggaca
      421 gcacccatta gaagtggaga gggggttttg gagtcatatt gggctctggc ccccgttcta
      481 tatgtgctta ttgctataaa aaatgtagca atttcacttt cgtataccag tacaaacctc
      541 ttgcatcgtg caaggaagcc aggtggtttt tccactgctg tcatggcatg atggctttca
      601 agaaaaattc cggaccatcc atgcgggaga catgtacttg cgaatagcca ctcacttggc
      661 tgcgctgggc gctttgatgg cgcaaactgc gcatgcccgg gacggcgctc ttgcctccgg
      721 ctggctggcc gatggctggg agattgtctg gaatatggct ttgccgccct tgtgcatggc
      781 cgtggtgatt ctgatctggc agggctacac gcgaattcgg cacgggcgtt cagccgatgt
      841 gttccagcca gagaaccgcg gccctctgct ctgggccctg ctggcgttct ccgcggggat
      901 ggcaggattt gcataccagg cacagacgac ggacaaggcg gtgcatgacg atgcctggaa
      961 gcgttttcag gccaatgtgg accgtgtgga ggctgatgtt ctgggcaagt ttgaaggact
     1021 gctccctccg ttgcgcggtg cccgtggtgc gctggtttcc agtgaatacc tgtcgcgcag
     1081 tgaattcagg aaattcatgc agtccaggga tatcgaccag gagttccccg gcgtgcgcgg
     1141 gctgggcatc atcgacagga ttccacgcac cggtttgaac gcgtttgttg ccgcacaacg
     1201 caaggacggg gcgcccgact ttacggtgaa gacttccggc acggcttcag accttttcat
     1261 tatcaaatac gtggagccgg tggagcgcaa ccgccaggcg gtgggactgg atgttggcgc
     1321 ggagccggtg cgccgggcgg ctgcagagcg tgccgtacgc cagggcaaac ccgctttgac
     1381 cgggcgcatc acgctggtac aggatgacca gaagagggcc ggatttctgt acttcctgcc
     1441 gtattacggg tctgtgcgcg ttcctgagac cgaggccgag cgcatccgcc agcttcgggg
     1501 ctggacctat gcccctattg ttctggctga gctcatggtg ggcacgcagc gaagtctgca
     1561 gggacagaca gagttccagc tgtttgaagg cgcagaagtt gccgcatcca agctgctatt
     1621 tgacagcacg cttccgctgg gagccaccga ggtgccggac ggcgtggacc atgtgcgcaa
     1681 gcacatgttc cataccgtcc gtccgctgtt gattggcggg cagttgctga ctttgcgtgc
     1741 cagtgcatct gatgactttg caagcaccct ggacatccag gcacctgcgc gcatggcact
     1801 ctatggatcc gtgtgcagtg tgctggctgg agccctggtc tggctgctgt tttcagcgcg
     1861 cgtccgtgtc gtcgcgacgg ctggacgcat gactgccgag ttgggacgtt tggctctggt
     1921 ggccaagggc accagcaacg cggtggtgtt taccgatgtc cgcggcctga cggtgtgggt
     1981 gaatgagggg ttcaccagga ttacgggcta caccagtgac gaagtgatgg gcaaagcgcc
     2041 cgggtatttg ctgcaaaacc acaacacaga tcctgccgtg gtgcgggcta tcggagatgc
     2101 cgtgcgtgcc cgcaagggcg gaaggtttga aatcctgaac cagcacaagg acggccaaga
     2161 atactgggtc agcctggaga ttcaacccgt gtgcgatgca cgaggcgagg tcaatggctt
     2221 catggccatc gagaccgaca tcactgcaga gaagggggcc aaggaggccc tggtgcggga
     2281 aaaagagcgg tttgaccgca ttctttccgg caccaacgtg ggcacctggc agtccaacct
     2341 gcagacgggc gaatcgcagt gcaacgcccg ctgggccggg atgatgggct ttgagcccca
     2401 tgaagtagtg ccgaacgccg atgtcttctg gacgcagcgt gtgcatccgg aagatctggt
     2461 gcgcaccacc aacgccacgc agcgttgccg gcaggggctc gcggacgaat actcgaccga
     2521 agtgcgggta aagcgcaagg acgacagctg gatgtggatt ctgagccggg ccaaagtgat
     2581 gtcccgcact gccgagggcg atgtcgagtg gattggcggc atccataccg acatttcgga
     2641 taccaaggcg ctggagattg gcctgcggga catggagtcg tttctgcacc gggccggacg
     2701 gctggcaggt gtcggtgcgt gggaagtgga cctgaaaacc ggcgaggtga aatggagtga
     2761 gcaaacctgt ctgatccacg gtgtggagcc cgacttcaaa ccgactgttg aagaagccct
     2821 gagtttctat accgagcagg ggcgtattga aatgcaggct gccatccaac ggtctattga
     2881 catggctctg ggctgggacg tcacgacgca aattctcact gcacaggggc agttgcgctg
     2941 gatccgggtt gcgggtgagc cggagtttga cgatagcggt gccgtgcggt tggtgggggc
     3001 attccaggac gtcaccgagc agcgtgaaac ccagctcagg attgagcgta gcgaggccat
     3061 cctgcgcgca tcgattgatg cggtgagtga tgcctatgtg ctgtacgacc cgcaggaccg
     3121 gctggcgtac tgcaacgaaa agtaccggga gttgtacgcg gcgtcgtccg atctgattgt
     3181 tcccggagcg accttcgaga gcatcattcg gggtggagcc gagcgggggc agtacgtgga
     3241 agccaacggc cgggtggacg cctgggtggc cgagcgcatg cgggtgcatc tggagggcaa
     3301 caccaacatg gagcagcggc tggacaacgg ccgctggctg cgcgtggtgg agaggcggat
     3361 gcccgatgga catcgcgtgg gtttccgggt ggacatcacg gaactcaagc tggccaatga
     3421 agccacccag gctgccaacg caacgttggc ccgcaccagc gcgacgttgc aggctgtgct
     3481 ggactccgct gtcgaggtgg ggatcatggc caccgacctg gataagagag tgactgtttt
     3541 caacaggggt gctgaaaatc ttacgggcta tgcggccggg gatatgttgg gcagcagtgg
     3601 cttgggcagg ctgttcgagc gctcccagat ggatctggtg cgcgagtccc tttcactgca
     3661 gttggggcgg cagccgaacg acgatgaagt gttcctcgag gtggcgaaat gcgcggactc
     3721 acctgagtgg accctggtac gcaaggatgg gacgatgttc acatcttcga tggtgatttc
     3781 atcgatgcgg gacgcaggcg gcgcgttgtc cggatacatg gccattgtgt acgacgtaac
     3841 ccgccagaag gaatacgaag cgtctttgcg cgacgcgatg cggcgtgccg aggagtcgag
     3901 tgtggccaag agccagttcc tggccaatat gagccacgaa atccggacgc ccatgaacgc
     3961 cattctgggc atgctccaac tcttgcacag tacccgcctg tcgccgcagc aactggacta
     4021 tgcggacaag accgaaggtg cggcccgctc gctgctgggg ctgctcaacg acatcctgga
     4081 cttctcgaaa gtggaagccg gcaagatgca gctggagctg gagccatttg cgttaagccg
     4141 cctgctcgac gaactgtcca ccatcctgtc ctccaacctg ggcagcaaga atgtcgactt
     4201 gctgtttgat gtggatccgg atatccctgc aaccctggtg ggggatgcct tgcgcatcaa
     4261 gcaggtgttg atcaacctgg gtggcaatgc agtcaagttc acagagcaag ggcatgtgct
     4321 catcgggctg cggatgctgg cgcgccacac cgaccgtgtg cggctggaaa tctcggtggt
     4381 tgactccggc attggcatcg cacccgaaaa ccaggcccgt atttttaatg cctttaccca
     4441 ggccgagtcc aacaccacgc gccgctttgg gggcacaggt ctggggctgg tcatttcgaa
     4501 gcgactgatc cgcctgatgg gaggggaact cgaactcagc agtgcgctcg gcgagggcag
     4561 tactttccgg ttcgcgctgg aactgccggt tccgccggat gcggtggcgt ttggagagcg
     4621 cgcgcgcgca ttgtccgcaa ccgatgtggt gcagacgttg ctggtggatg acaacccggt
     4681 ggctctggag gtcaacgcgg ctgccatgcg cagcctgggg tggagtgtgg ttcaggctgc
     4741 gtcgggtgag caggcggttt cactggtcac acagcggcag gccgcgcaaa tgcctgcgtt
     4801 cgatgccgta tttgtggact ggcatatgcc cggtatggat ggctgggaaa ccttgcgcat
     4861 gatccgcagg gtcgtgggca gcggaccagg catggcattg gtactgatga gcgggcaaag
     4921 ccgtgccgcc ctctttgagc ggaccgagcg cgagcaggcg ctgctcaacg gctacatggt
     4981 caaacccctg acggcagaca tgttccgtga tgcgctggcg caggcctacg atgcgccgta
     5041 tgtcgagtcc acgcaaacca atctgggaag tgcaggggat gccgtagacg tgacgcccct
     5101 caagccactt ggcgggatgc gcattctggt ggtggaagac aacccgatca accagcaggt
     5161 agccagcgaa ctgttgcgtg cgcaaggtgc cagcgtgacc ctggcaaata acggtcagga
     5221 aggtgtggac gcggttctga ctgcgaaccc gccctttgac gtggtgctga tggacctgca
     5281 gatgccggtc atggatgggc tgactgcagc gcgcaccatc cgcagccatg cccggtgggg
     5341 tggcctgccg atcattgcca tgaccgccaa tgcgatggcg tctgaccgtg aggcctgcct
     5401 ggaggctggc atgaacgacc atgtggccaa gccgtttgac ctgaacgacc tggtgaagac
     5461 gctggtggac catacgcatt ggacaccgcg tggggaagtg ccggttggta ctcccgccga
     5521 agtgccccct gcgggcggca accggaatct gctgggtcag atctggccgt ctggcgttga
     5581 tgtagaagct gctttgaacc gcatgggagg caatgcgggc ctgttgcagc gctccatcgc
     5641 agcgttcttg cggtatgcag aagtgttgcc agagcgtgta cggggatggc tggacagcca
     5701 cagcctggca gacgtgcggc g
//
LOCUS       DAOSJM010000135         5707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12716_length_5707_cov_3.826964, whole genome shotgun sequence.
ACCESSION   DAOSJM010000135 DAOSJM010000000
VERSION     DAOSJM010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5707
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12716_length_5707_cov_3.826964"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PKX82_06150"
     CDS             complement(<1..343)
                     /locus_tag="PKX82_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF51870.1"
                     /translation="MSASKLHAAGVLQGGRWRWWEVVLWAAALASPWALSSHSLIINE
                     IAILALFALSLDLILGYTGIVSLGHAAFFGMGAYAAALFSKLVMPDPLVGLMVGIACA
                     TVLGAICSVTIL"
     gene            complement(340..1215)
                     /locus_tag="PKX82_06155"
     CDS             complement(340..1215)
                     /locus_tag="PKX82_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF51871.1"
                     /translation="MLTILFDGVAYGMLLFILAVGLAVTMGLMNFINLAHGAFAMVGG
                     YITVLLMQRLGVPFLLCLPLAFLGSALLGGVLERTLYCLLYHKPHLDQVLFSIGLTFM
                     AVAAVDYFVGSTQQNLQLPEWLKGRTELGEGDWMLGMGHYRLFIIAVCAALTVALQYI
                     LTKTRFGSRLRASVDDQRVAAGLGINVNVVFLSTFAVGSGLAGLGGALGADVLGMDPS
                     FPLKFMIYFLIVVAVGGTSSITGPLLAALLLGVADVAGKYYIPKMGAFIVYFLMILIL
                     VWRPQGLFVRQGGKA"
     gene            complement(1320..2492)
                     /locus_tag="PKX82_06160"
     CDS             complement(1320..2492)
                     /locus_tag="PKX82_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF51872.1"
                     /translation="MQKRIFLTGTALAALSLVAPAVMAQADVFKIGLILPMTGQQATT
                     GRQIEAAAKLYMAQNGDMIAGKKVQLIVKDDTSIPDVTKRMAQELVVNEKVNVLAGMG
                     ITPSALATAAIATQSKTPLVVMAAATSSITQASPYVVRTSFTLRHASVSLADWAPKNG
                     IKKVVTLVSDYGPGIDAEKYFKERLTFNGGQVTESLRVPLRNPDFAPFLQKVRDLKPD
                     ALFVFVPSGAGTAVMKQFLERGMDKAGIKLIGTGDVTDDDLLNDMGDGALGVVTSHHY
                     SAAHPSAMNKKFVEAFKKANNNLRPNFMAVGGYDGMRVIYEALKATKGQGGGDALLAA
                     MKGQIFESPRGPMFIDAQTRDVVQNIYLRKVERKDGQLYNVEFDVIKDVKDPGKAK"
     gene            2672..3781
                     /locus_tag="PKX82_06165"
     CDS             2672..3781
                     /locus_tag="PKX82_06165"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HOF51873.1"
                     /translation="MTQLPAHYDHVGSFLRPKYLLDARDQRAAGTITSEQLRLVEDKA
                     IAEIVKFQEDVGLKSITDGEFRRTYFHIDFLEQIGGVKTDIPVTIQKPDGTQELAPPV
                     MRVIDKVRHVKDIQLADFQFLKSQVSQGNTPKVTIPSPTMLHFRGGRAGISREAYPEL
                     DPQFYDDVAQAYGDELRSLAAAGCTYVQMDDTNLAYLCDEKMREAARLRGDDPNELPH
                     RYAAFINKVVAQKPTGMLLAMHLCRGNFKSTHAASGNYEPVAEALLKEMNLDAYFMEY
                     DDDRSGDFKPLRFLPKGKTVVLGLVTTKFGEMESKDTLKRRIDEAAKYAPLEQLALSP
                     QCGFSSTVHGNNIAVEAQRAKLRLVIETAQEVWGS"
     gene            complement(3798..4301)
                     /locus_tag="PKX82_06170"
     CDS             complement(3798..4301)
                     /locus_tag="PKX82_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51874.1"
                     /translation="MSRALKFLMFIFFVVFITTGAFMVLIVNADDASQPWISLLVRNH
                     GIVFVWHLACWMTMGMFSNYYWDLFNMGKGLDGMQVERIVLPLLVSPIVFYPTYNLWL
                     SSSSQSYILFEVIAFQSGFFWQALFTKAKPLESATTIPWTVNNGSGRMGGKVGAVAGQ
                     QPQDVAR"
     gene            complement(4367..5005)
                     /locus_tag="PKX82_06175"
     CDS             complement(4367..5005)
                     /locus_tag="PKX82_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOF51875.1"
                     /translation="MAWNQDQMAARAARELEDGFYVNLGIGIPTLVANHVPADKEVWL
                     QSENGMLGIGPFPTDDEVDADLINAGKQTVTTIKGSSIFGSHESFAMIRGGKINLSIL
                     GAMQVSEKGDLANWMIPGKMVKGMGGAMDLVAGVKRVIVLMEHVAKKKDGTEDMKILP
                     HCTLPLTGKAVVDRIITDLAVMDVTRHGLIVVELAPGVTREFLQSKTGVTLI"
     gene            complement(5008..5706)
                     /locus_tag="PKX82_06180"
     CDS             complement(5008..5706)
                     /locus_tag="PKX82_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOF51876.1"
                     /translation="MYPNAAAALQGIVRDGQLLAVGGFGLCGIPEALIDALRDTGAKD
                     LTVISNNAGVDGFGLGKLLDTRQIKKMISSYVGENKEFERQYLAGELQLEFTPQGTLA
                     EKLRAGGAGIPAFFTKTGVGTIVADGKELREFDGETYVMEHALVPDVALVKAHRADKS
                     GNLQFRFTARNFNPAAAMAGKICVVEVEEIVETGDIPPDQVHLPGIYVHRIVLNATPE
                     KRIEKRTITEKAGV"
BASE COUNT         1214 a         1780 c         1660 g         1053 t
ORIGIN      
        1 gcaggatggt cacgctgcaa atggcaccga gcaccgtggc acaggcaatg cccaccatca
       61 ggcccaccag cggatcgggc atgaccagct tggagaacag cgcagcagca tacgcgccca
      121 tgccgaagaa cgccgcgtgc cccagagaca cgatgccggt gtagcccaga atcaggtcca
      181 gtgacagcgc aaacagcgcc aggatggcga tctcgttgat gatcagcgag tggctggaca
      241 gggcccaggg cgatgccagc gcagcagccc acagcaccac ctcccaccag cgccagcgac
      301 caccctgcag cacaccggcc gcatgcaatt tggacgcgct catgctttgc ctccctgtcg
      361 cacaaacagg ccttgcggcc gccacaccag aatcagaatc atcaggaaat acacaatgaa
      421 agcacccatc ttggggatgt aatacttgcc cgccacgtcg gccacgccca gcagcaatgc
      481 ggccagcagc ggaccggtga tgctggaggt gccgcccacg gcgactacga tcaaaaagta
      541 aatcatgaac ttcagcggaa agctcgggtc catgcccagc acatccgccc ccagcgcacc
      601 gcccagaccg gccagacccg aacccacggc aaaggtggac agaaacacca cgttcacatt
      661 gatgcccaga ccagccgcca cgcgctggtc atccaccgat gcacgcaggc ggctgccaaa
      721 gcgggtcttg gtcaggatgt actgcagggc tacggtcagt gcggcacaca ccgcaatgat
      781 gaacaggcgg tagtggccca tgcccagcat ccagtcgccc tcgcccagtt cggtgcgacc
      841 cttgagccac tcgggcaact gcaggttctg ctgggtggag cccacgaagt aatccaccgc
      901 agccacagcc atgaacgtca agccgatgga gaacagtacc tggtccagat gcggcttgtg
      961 gtacaacaaa caatacagcg tgcgctccag cacgccaccc agcaaggcgg agcccagaaa
     1021 cgccagcggc aggcacagca gaaacggcac acccaggcgc tgcattagca gcaccgtgat
     1081 gtagccgccc accatggcaa aggcgccgtg ggccaggttg atgaagttca tcaagcccat
     1141 ggtcaccgcc aggcccactg ccaggatgaa cagcagcatg ccgtaggcaa caccgtcaaa
     1201 aagaatggtc aacatagttt gcgctcacaa cgaggggcgt tgggtcgtaa aaaaagcgaa
     1261 gggggaccct cgcttttctg gaatcaaccg ggtccagcac gctgcgaccc gatctgtggt
     1321 tacttggcct tgccggggtc tttgacatcc ttgatcacat cgaactcgac gttgtacaac
     1381 tggccgtcct tgcgctccac cttgcgcagg tagatgttct gcaccacgtc gcgggtttgc
     1441 gcgtcaatga acattgggcc gcgcgggctc tcaaaaatct ggcccttcat ggccgccagc
     1501 aatgcgtcgc caccgccctg gcccttggtg gccttgaggg cttcgtagat cacgcgcatg
     1561 ccgtcatagc cacccacggc catgaagttg gggcgcaggt tgttgttggc cttcttgaag
     1621 gcttcaacaa acttcttgtt catggccgag gggtgcgctg ccgaatagtg gtgcgaagtg
     1681 accacgccca gcgcgccatc gcccatgtcg ttgagcaaat cgtcatcggt cacgtcaccg
     1741 gtaccgatga gcttgatacc ggccttgtcc atgccgcgct ccagaaactg cttcatgacc
     1801 gcagtgccgg caccagaggg tacaaacacg aacagcgcat ccggcttgag gtcacgcacc
     1861 ttctgcagga acggggcaaa gtcaggattg cgcagtggca cgcgcaggga ttcggtgacc
     1921 tggccgccgt tgaacgtcag gcgctccttg aaatactttt cagcatcgat gccaggaccg
     1981 tagtcgctga ccagggtcac gactttcttg ataccgttct tgggggccca atcggccagc
     2041 gacaccgaag catggcgcag cgtgaaactg gtgcgcacca catagggcga cgcctgggta
     2101 atgctcgaag tggcggcggc catcacgacc agcggagttt tggactgggt ggcaatggcc
     2161 gcagtggcca gggccgatgg ggtgatgccc atgcccgcca gcacattcac tttttcattc
     2221 actaccagtt cctgggccat gcgcttggtc acgtccggaa tgctggtgtc gtccttgacg
     2281 atgagttgca ctttcttgcc ggcaatcatg tcgccgtttt gcgccatgta cagcttggca
     2341 gccgcttcaa tctggcggcc ggtggtggcc tgctggccgg tcatgggcag gatcagacca
     2401 atcttgaaca catcagcctg cgccatgacg gcgggagcca ccaacgacag ggctgcgaga
     2461 gccgttccgg tcagaaaaat tcgcttttgc atttttgtct cctgcagggt tgaaggggtg
     2521 aactggccgt gactatggcc ctgaattgcc aatgccgcaa tcgtgatttc aaactgttag
     2581 atataacgat ttgcaatagc tcaacagcac tacccagagt atccttcaga tcacccgcga
     2641 ttcccataag ttcaccaaag aggagacacc cgtgacccaa ttgccagccc actacgacca
     2701 cgtcggaagc tttctgcgcc ccaagtacct gctcgatgcc cgcgaccagc gcgcagcagg
     2761 aaccatcacc tcggagcagt tgcgtctggt ggaagacaag gccatcgccg aaatcgtgaa
     2821 gttccaggag gacgtgggac tcaagagcat taccgacggc gagttccgcc gcacctactt
     2881 ccacatcgac tttctggagc agattggcgg cgtcaagacc gacattccgg tcaccatcca
     2941 gaaacccgat ggcacccagg agctggcacc gcctgtcatg cgcgtgatag acaaggtacg
     3001 ccacgtcaag gacatccagc tggccgactt ccagttcctc aagagccaag tcagccaagg
     3061 caatacccca aaagtgacga ttccctcccc caccatgctg catttccgcg gtggtcgcgc
     3121 cggtatcagc cgcgaagcct accccgagct cgatccccag ttttacgacg atgtggccca
     3181 ggcctatggc gacgaattgc gctcgctggc agcagccggt tgcacctatg tgcagatgga
     3241 cgacaccaac ctggcctacc tgtgtgacga gaagatgcgt gaggccgccc gcctgcgtgg
     3301 cgatgacccc aatgaactgc cccaccgcta tgccgcgttc atcaacaagg tggtggcaca
     3361 aaagccgacg ggcatgctgc tggccatgca cctgtgccgc ggcaatttca agagcaccca
     3421 cgccgccagc ggcaactacg agcccgtggc cgaggcactg ctcaaggaaa tgaacctgga
     3481 cgcgtatttc atggaatacg acgatgaccg cagtggcgac ttcaagcccc tgcgcttcct
     3541 gcccaaaggc aagaccgtgg tgctgggcct ggtgaccacc aagtttggcg agatggaaag
     3601 caaggacacg ctcaaacgtc gcattgacga ggctgcgaaa tatgcgccgc tggagcagct
     3661 ggcgctgtca ccccagtgcg gcttttcgag cacggtccac ggcaacaata ttgcggtgga
     3721 agcccagcgc gccaagttgc ggctggtgat tgaaacggcg caggaggtgt ggggctccta
     3781 gccccacctg ggaagggtca gcgggccacg tcctgtggct gctggcctgc tacggcgcca
     3841 actttgccgc ccatgcgtcc ggagccgttg ttgacggtcc agggaatggt ggtggcagac
     3901 tccagcggct tggccttggt gaacagggcc tgccagaaaa atccgctctg gaacgcaatc
     3961 acctcaaaca ggatgtagct ctgcgaactg ctggagagcc acaggttgta ggtggggtag
     4021 aacacgatgg gcgacaccag caacggcagc acgatgcgct cgacctgcat gccatccagg
     4081 cccttgccca tattgaacag gtcccagtag tagttggaaa acatgcccat ggtcatccag
     4141 cacgccaggt gccagacaaa cacgatgccg tggttgcgca ccagcaggct gatccagggt
     4201 tgggatgcgt catccgcatt cacgatgagc accatgaacg cccccgtggt gatgaacacc
     4261 acgaagaaaa tgaacatcag aaatttgagg gctctggaca tgggatctcc gcaagatggt
     4321 gggcacctgt tggtgcgcgc accatcttac ccgctgtccg gggctgtcag atcaaggtca
     4381 caccagtttt tgactgcaaa aactcgcggg tcacgcccgg tgccagctcc accacgatca
     4441 gaccgtggcg ggtcacgtcc atcaccgcca gatcggtgat gatacggtcc accaccgcct
     4501 tgccagtcaa cggcagcgtg caatgcggca ggatcttcat gtcttcggtg ccgtctttct
     4561 ttttggccac gtgctccatc agcacaatca cgcgcttgac gcctgccacc aggtccatcg
     4621 caccgcccat gcccttgacc atcttgccag ggatcatcca gttggccaga tcgccttttt
     4681 cgctgacctg catggcgccc aggatcgaca ggttgatctt gccgccgcgg atcatggcga
     4741 aactctcatg gctgccaaaa atgctggagc ccttgatggt ggtgacggtc tgcttgccgg
     4801 cgttgatcag gtcggcatcg acctcatcgt cggtaggaaa cgggccgatg cccagcatgc
     4861 cgttttcgga ctgcagccag acttccttgt cggcaggcac atggttggcc accagcgtgg
     4921 gaatgccgat acccagattt acatagaaac cgtcttccag ttcccgggct gcgcgggcag
     4981 ccatttggtc ttgattccat gccatgttca gactcctgct ttctcggtga tggtgcgctt
     5041 ctcgatccgt ttttccgggg tggcgttgag cacaatgcgg tgcacataga tgcccggcag
     5101 gtgtacctgg tccggtggaa tatcgccagt ctcgacaatc tcttccactt cgacgacaca
     5161 gatttttccg gccatggcgg cggcggggtt gaagttgcgc gcggtgaagc ggaactgcag
     5221 attgccggac ttgtcggcac ggtgcgcctt gacgagcgcc acgtcgggca ccagggcgtg
     5281 ctccatcaca taggtctcgc cgtcgaactc gcgcagttcc ttgccgtcgg ccacgatggt
     5341 gcccacgccg gtcttggtga aaaaagccgg gatgccagcg ccgccagcac gcagcttttc
     5401 ggccagcgtg ccctgggggg tgaattccaa ttgcagttcg ccggccaggt actggcgctc
     5461 gaactccttg ttctcgccca cgtagctgga gatcatcttc ttgatctggc gcgtgtccag
     5521 caacttgccc aggccaaagc cgtcgacgcc ggcgttgttg gaaatcacgg tgaggtcctt
     5581 ggcgccggtg tcgcgcagcg catcgatcaa agcctcgggg atgccgcaca ggccaaagcc
     5641 gcccacggcc agcaattggc cgtcgcgcac gatgccctgg agcgccgccg cagcgttggg
     5701 ataaatc
//
LOCUS       DAOSJM010000136         5692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12764_length_5692_cov_4.497428, whole genome shotgun sequence.
ACCESSION   DAOSJM010000136 DAOSJM010000000
VERSION     DAOSJM010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12764_length_5692_cov_4.497428"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PKX82_06185"
     CDS             complement(<1..925)
                     /locus_tag="PKX82_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOF51877.1"
                     /translation="MLDEVEGIVQGHRDGHGFVSRDDGAPDIYLPPNEMRAVLHKDRV
                     KVRIVRNDRKGRPEGRVVEIIERSSQPIIGRLLQESGVWLVAPEDKRYGQDVLIPKGA
                     TSVAKPGQVVVVELVEPPALFGQPVGRIKEVLGEVDDPGMEIEIAVRKYSVPHEFSEA
                     CIALARTLPDKVRPHDHANRVDLTDIPLVTIDGEDARDFDDAVYCEPATKGRGKSATK
                     GWRLLVAIADVSHYVETGSAIDVDAYDRATSVYFPRRVIPMLPEKLSNGLCSLNPEVE
                     RLCMVCDMFVTEMGDVEAYQFYPAVMWSHSRF"
     gene            975..1059
                     /locus_tag="PKX82_06190"
     tRNA            975..1059
                     /locus_tag="PKX82_06190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1009..1011,aa:Leu,seq:cag)
     gene            complement(1167..2564)
                     /gene="rimO"
                     /locus_tag="PKX82_06195"
     CDS             complement(1167..2564)
                     /gene="rimO"
                     /locus_tag="PKX82_06195"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOF51878.1"
                     /translation="MSEAKPTTSASVPKVGFVSLGCPKALTDSELILTQLSAEGYQTS
                     KTFQGADIVIVNTCGFIDDAVKESLDTIGEALAENGRVIVTGCLGAKQSEGGENLIRQ
                     VHPKVLAVTGPHATQEVMDAVHLNLPKPHDPFIDLVPNAFGVAGIKLTPRHYAYLKIS
                     EGCNHRCTFCIIPSMRGDLVSRPVGDVLSEARALFEGGVKELLVISQDTSAYGVDVKY
                     RTGFWDGKPVKTRMLELVATLGEIAESYGAWVRLHYVYPYPSVDEIIPLMASGRILPY
                     LDVPLQHSHPDVLKRMKRPASGEKNLERIQQWRQACPQLVVRSTFIAGFPGETEEEFS
                     HLLDFMQEAQIDRAGCFAYSPVSGAAANDLPGMLPAEVREERRARFMAVAEQISAERL
                     QRRVGSVMQVLVDSAPAMGRKGGVGRSYADAPEIDGVVRLLPPEKISKTLKVGEFTKA
                     RIVSAQGHDLVALPI"
     gene            complement(2607..3056)
                     /gene="phaR"
                     /locus_tag="PKX82_06200"
     CDS             complement(2607..3056)
                     /gene="phaR"
                     /locus_tag="PKX82_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HOF51879.1"
                     /translation="MQRKPFKDSKSAERVIKKYPNRRLYDTDTSSYITLAEIKQLVMD
                     SEPFVVRDAKTGDDLTRSILLQIILEEEAHGSPMFTAPVLSNIIRFYGHAMQGMMGGY
                     LEKNMQALMDLQAPMVQGVMGNNVFQQMQEHMQKQTEQFLGTFGIKR"
     gene            complement(3228..3304)
                     /locus_tag="PKX82_06205"
     tRNA            complement(3228..3304)
                     /locus_tag="PKX82_06205"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3268..3270),aa:Val,seq:gac)
     gene            3449..4207
                     /locus_tag="PKX82_06210"
     CDS             3449..4207
                     /locus_tag="PKX82_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOF51880.1"
                     /translation="MEIAGKVFIVTGGASGLGEGTARMLAGKGGKVVIADMQVEKGEA
                     IAKEIGGAFVKCDVSNEADGQAVVAKAVSMGKLMGLVNCAGIAPGEKTVGKNGAHALS
                     TFTKCITVNLVGSFNMIRLAADAMCKNEPEATGERGVLISTASVAAYDGQIGQAAYSA
                     SKGGIVGMTLPIARDLARNGIRNMTIAPGIFGTPMMFGMPKEVQDSLAAAVPFPSRLG
                     TPQDYAKLAQHIFENDMLNGEVIRLDGAIRLAPK"
     gene            complement(4504..5286)
                     /locus_tag="PKX82_06215"
     CDS             complement(4504..5286)
                     /locus_tag="PKX82_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF51881.1"
                     /translation="MAYTIDLSGRVAFITGASSGLGAQFARTLAGAGAAVVLASRRVD
                     KLKELRATIEGEGGDAHVVELDVTDHDSIKSAVAHAETEVGSIDILINNSGVSTTQRL
                     QDVSPDDYDFMFSTNVKGAFFVAQEVGKRMLARAKGSAPGNYTGGRIVNIASMAGLRV
                     LPQIGVYCMSKAAVIQMTKAMALEWGKFGINVNALCPGYIDTEINHHHWQTEGGQKLV
                     QMLPRKRVGNPKDLDALLVMLCSDQSHFINGAVIAADDGFGI"
     gene            5426..>5692
                     /locus_tag="PKX82_06220"
     CDS             5426..>5692
                     /locus_tag="PKX82_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51882.1"
                     /translation="MTNEEILTQFGPREAMEYDVVVVGGGPGGLATAIRLKQLAAEKG
                     TDVSVVVLEKGSEPGAHILSGAIMDPKALTELFPNWKELGAPLNQ"
BASE COUNT         1265 a         1701 c         1493 g         1233 t
ORIGIN      
        1 taaatcggga atggctccac atcacagcgg gatagaactg gtatgcctct acatcaccca
       61 tctccgtgac gaacatatcg cacaccatgc acagtcgctc gacctcaggg ttgagcgaac
      121 acagtccgtt ggagagcttt tccggcagca tgggaataac tcgacgcgga aaatagacgc
      181 tggtagccct gtcgtaggca tcaacgtcaa tcgcagaacc tgtttccacg tagtggctca
      241 catcggcaat ggctacgagc agtcgccagc ccttggtcgc tgatttccct ctacccttgg
      301 tggcgggttc gcaatacacc gcatcgtcaa agtctcgcgc atcttcccca tcaatggtga
      361 ccagaggaat atcggtcaga tcgacgcgat tggcatggtc gtgtggccgg actttgtccg
      421 gcagggttct ggccagcgca atgcacgctt ctgaaaactc atgcggcacg ctgtatttgc
      481 gcaccgcaat ttcaatctcc atgccgggat cgtcaacttc ccccagcact tccttgatgc
      541 gacccaccgg ttgtccgaaa agagctggtg gttctaccag ttccacaacg acaacctgcc
      601 cgggcttggc aacactggtg gcgcctttgg gaatcaggac gtcctgccca taccgtttgt
      661 cttcgggcgc caccagccac acgccacttt cctgcagcag gcgaccaatg atcggttggg
      721 acgatcgctc aatgatttcc acaacgcgcc cttcagggcg tcccttgcgg tcattgcgca
      781 cgatgcgcac tttgacacgg tccttgtgca gcactgcccg catttcgttc gggggcaaat
      841 aaatgtccgg agcaccatcg tcgcgggaca cgaatccgtg gccatcgcga tgtccctgaa
      901 caattccttc aacttcatcc agcattccgc tgttttggct caaatttttg atatacttct
      961 ctctttcctg agatgcccag gtggcggaat tggtagacgc actagtttca ggtactagcg
     1021 agtaacatcg tggaggttcg agtcctcttc tgggcaccaa caatggtaat gtagatattg
     1081 aaaagccgct gtaaacacag cggctttttt ttgtctgttt gattcatgcc acggaccata
     1141 gtttgcatga gtccgtggcc ttgtgatcag atgggcaaag ccacgaggtc gtgaccttgc
     1201 gcgctgacga tacgcgcctt ggtgaactct ccgaccttta gcgtcttgct gatcttttct
     1261 ggcggcaaca aacgcacgac gccatcaatt tctggcgcat ctgcataact ccgccctact
     1321 ccgcctttac gacccattgc tggcgcggaa tccaccagca cctgcatgac agaccccacc
     1381 cggcgctgca aacgctccgc cgaaatttgc tctgccactg ccatgaaccg tgcgcggcgc
     1441 tcctcacgca cttctgcggg aagcatgcca ggcaaatcat tggccgcagc accactcaca
     1501 gggctgtacg caaagcaccc ggcccggtcg atctgcgcct cctgcatgaa atccagcaag
     1561 tgactgaatt cttcttcggt ttcccctgga aatccggcga tgaaggtgct gcgcacgacc
     1621 agttggggac aagcctgtcg ccattgctgg atacgttcga gatttttctc accgctggct
     1681 ggacgtttca tgcgcttgag tacatccgga tggctgtgct gcaacggtac atccagatac
     1741 ggcaggatcc gcccggacgc catcaacggg atgatctcgt ccacactggg atacgggtaa
     1801 acgtaatgca aacgaaccca cgcgccgtag gactctgcaa tctcaccgag tgttgccacc
     1861 aactccagca tgcgtgtctt gaccggcttg ccatcccaga acccggtgcg gtacttcacg
     1921 tcaactccgt acgccgaagt gtcctggctg atcaccagca actccttgac acccccttca
     1981 aacaaggcac gcgcttccga cagcacatct ccaacaggac gcgaaaccaa atcaccccgc
     2041 atcgacggga tgatgcaaaa ggtgcaacgg tggttacagc cttcgctgat tttcaaatag
     2101 gcgtaatggc gcggtgtcag tttgataccc gccaccccga aggcattggg aaccagatca
     2161 atgaaagggt catggggctt gggaaggttc aggtggacag catccatcac ttcctgcgta
     2221 gcgtgcggcc cggtcaccgc cagcactttg ggatgcacct gacggatcag gttctctccc
     2281 ccttcagatt gcttggcacc caaacagccg gtcacaatga cccggccatt ttcggcgagc
     2341 gcttctccaa tggtgtccag gctttccttg accgcatcat caatgaaccc acaggtgttg
     2401 acgatcacga tatcggcacc ctggaaggtc tttgaggtct ggtagccctc cgcactcaat
     2461 tgggtcaata tcagttcgga gtcggtcagt gccttggggc aaccaagact gacaaagccc
     2521 actttaggga ctgatgcact ggtggttggt tttgcttcac tcatgcaccg attgtcccag
     2581 cattccagcc gggaagtgca caacgcttat cgcttgatgc caaaagtacc cagaaactgt
     2641 tctgtctgct tctgcatatg ctcctgcatc tgctggaaca cgttgttgcc catgacgcct
     2701 tggaccatgg gtgcctgtaa atccatcagc gcctgcatgt tcttttcgag gtacccaccc
     2761 atcatgccct gcatggcatg gccatagaaa cgaatgatgt tggacagcac tggcgccgta
     2821 aacatcgggg atccgtgggc ctcctcttcc agaatgattt gcaggaggat gctgcgcgtc
     2881 agatcgtcgc ccgtcttggc atcgcgcacc acaaacggct cactgtccat caccagctgt
     2941 ttgatctctg ccaaggtgat gtaactcgaa gtgtcagtgt catacagacg gcgattcggg
     3001 tatttcttga tgacccgttc agccgattta ctgtctttga atggtttgcg ttgcattgcg
     3061 tgcggattgg gcagccattg ctaccgagta tgtattgcag tgcagcacat tgtagagacc
     3121 ggtaccagtg caatgaccta ggtttaccct agtggcaaca ggtccgtcaa gacacgaacc
     3181 cagaaacaaa aacggcacca catggtgccg ttgcttcagg aagatgttgg taggcgcaat
     3241 tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccagc tgagctatgc
     3301 gcctacatat ccgagagggc agagtatagc agtaaacaat agagctcctg aaattttcat
     3361 ccgtatgagt gcgcaccttc gtggtcggtg ataatttacg ttaacgtaaa cgtcaattcc
     3421 caccatcact tcacactagg agcaattcat ggaaatcgca ggaaaagtat tcatcgtcac
     3481 cggtggcgca tcgggtctgg gcgaaggcac agcgcgcatg ctggctggca agggcggcaa
     3541 ggtggtcatt gcagacatgc aggtcgagaa aggcgaagct atcgccaagg aaatcggcgg
     3601 cgcgtttgtc aaatgcgatg tgagcaatga agccgatggc caggctgtcg tcgccaaggc
     3661 tgtatccatg ggcaagctca tgggcctggt caactgtgcc ggcattgcac ccggtgagaa
     3721 gaccgttggc aagaatggcg cacatgcgct gtccaccttc accaagtgca tcaccgtcaa
     3781 tctggtgggc agcttcaaca tgatccgcct ggctgctgat gccatgtgca aaaacgaacc
     3841 cgaagctaca ggcgagcgtg gtgtgttgat ctccactgcc tccgttgctg cgtacgacgg
     3901 ccagattggt caggctgcgt acagcgcgtc caagggtggc attgtgggta tgaccttgcc
     3961 catcgcccgt gatctggcgc gcaacggcat tcgcaacatg acgattgccc ccggcatttt
     4021 tggcacaccc atgatgttcg gcatgcccaa ggaagtgcag gactctctgg ccgctgcggt
     4081 gcctttccct tcccgcctgg gcacccctca ggattacgcc aaactggcac aacacatctt
     4141 tgaaaacgac atgctcaacg gcgaagtgat ccgccttgac ggcgccatcc gcctggcacc
     4201 gaagtaagta cccgggcacc aacgagccgc cgtaccaacg gagcggcagc tcttgggggc
     4261 cttgctacct gtgcgcgcga atggaagcca ggatgcccag tacaagcaca ttaccagcca
     4321 ctaattggct tgtaaaattt cgcagcacac tggcatccat gccgatcccg ctcatactgc
     4381 tacacaaacc atagcagact atgcaacatt ctcctgggct agaagcatat ttagctccgt
     4441 acccagcaaa caccagacaa gaaatccggc cagtatcccc gtcagcacaa gaaaccggag
     4501 aacttaaatg ccaaagccgt cgtccgcagc aatcactgcg ccattgataa agtggctctg
     4561 gtccgaacac aacatcacca gcagcgcatc cagatccttg gggttaccca cgcgcttgcg
     4621 cggcagcatc tgcaccagct tctgaccacc ttccgtctgc cagtggtggt ggttgatttc
     4681 ggtgtcgatg tagccggggc acagcgcatt gacattgatg ccaaacttgc cccactccag
     4741 cgccatggcc ttggtcatct ggatcaccgc agccttgctc atgcagtaca caccaatttg
     4801 cggcaacacg cgcaggcccg ccatcgatgc gatgttcaca atgcgtccac cggtgtagtt
     4861 gcccggcgca gaacccttgg cgcgcgccag catgcgctta cccacttcct gggccacaaa
     4921 gaaggccccc ttgacgttgg tgctgaacat gaagtcatag tcatcgggcg aaacatcctg
     4981 caggcgctgg gtcgtgctca cgcccgagtt gttgatcagg atgtcaatgg agcccacttc
     5041 ggtttcggca tgggccactg ccgatttgat ggagtcatgg tcggtcacat ccagttccac
     5101 cacatgggca tcaccgccct cgccttcaat ggtggcgcgc agttccttga gcttgtccac
     5161 gcggcgactg gccagcacca cggccgcacc ggcacccgcc agcgttcggg cgaactgcgc
     5221 ccccaagcca ctggaggccc cggtaatgaa ggccacgcgg ccggataaat cgatggtgta
     5281 tgccatttag agagtctcca cgaatcaaat acgaacgatc gttctttttt acctattcca
     5341 cgcatagaat ctgtcccaaa gctgaccgga tccacgcgtg attgttggca caatcgactg
     5401 aaccccatta tcaagcgaga cacccatgac caacgaggaa attctgaccc aatttggccc
     5461 ccgcgaggcc atggaatacg acgtggttgt cgtaggtgga ggccccggtg ggctggcaac
     5521 agccattcgc ctgaagcagc tggccgcaga aaagggcacg gatgtctccg tggtggttct
     5581 ggaaaaaggt tcggaacccg gcgcacacat tctgtcgggt gccatcatgg accccaaggc
     5641 gctgaccgaa ctctttccca actggaagga acttggtgcc ccgctgaacc ag
//
LOCUS       DAOSJM010000137         5684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12784_length_5684_cov_4.266655, whole genome shotgun sequence.
ACCESSION   DAOSJM010000137 DAOSJM010000000
VERSION     DAOSJM010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12784_length_5684_cov_4.266655"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1932
                     /locus_tag="PKX82_06225"
     CDS             <1..1932
                     /locus_tag="PKX82_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOF51883.1"
                     /translation="AIVVVENVERIMSEEGLPPLQATRKAMGQISGAIVGVTVVLIAV
                     FVPLAFFSGSVGNIYRQFSAVMVSSIAFSAFLALSLTPALCATLLKPVEAGHAHAKTG
                     FFGWFNRRFNSTAKGYESVVTRLLKKAGRYLIIYAAIIGAVAVVYMRLPTSFLPGEDQ
                     GSIIVNVQLPPGATQERTLSVMQQVEGFILKQPEVQSMVGVLGFSFSGQGQNAALAFV
                     TLKDWADRPGPGQSAEALAGRAFGALMGIRDAFIFPLSPPPIPELGSASGFTFRLQDR
                     AGKGHDALIGARNQLLGMASQSKVLAQVRPDGLEDAPQLQIDIDRDKASALGVSFDAI
                     NSTISTALGSSYINDFPNAGRLQRVVVQADATARMQPEDVLKLNAMNSQGKAVPLSAF
                     ASTRWIKGAMQTVRYNGYPAVRISGAAAPGFSTGAAMNEMEKLAAQLPAGFGFEWTGQ
                     SRQEKLAGSQAMVLYGFAILAVFLCLAALYESWSIPLAVILVVPLGVLGVVLATLMRG
                     YANDVYFQVGLITIIGLSAKNAILIIEFAKDLQAQGKSAVEAALAAAHLRFRPIVMTS
                     MAFVLGVLPLAIASGAGSASQRAIGTGVMGGSLLGTMLAVLFVPIFFVVVRSVFKGSE
                     RQHQFDMEHGHQIGVEKHE"
     gene            1925..3367
                     /locus_tag="PKX82_06230"
     CDS             1925..3367
                     /locus_tag="PKX82_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOF51884.1"
                     /translation="MSKSRMQHPALLRLVPLVAALALGGCSFIPTYERPAAPVAPKFS
                     AVASQAPAAAASTPVRQAADMEWQTFFKDARLKRLIELALQNNRDLRVAVLNIEQTRA
                     QFQVRKADELPTVNAGIAGSRGPTTTGAITSTYTAGLSVTAYELDFFGRVRALSQAAQ
                     AQLLGTEEARKTVQMALIASLANTYLNLLADDELLKVTRDTLATREESLRLTRLKFDN
                     EAASQLDLSQAQSALEGAKASLAQQTRQRALDENALVLLVGQTLPADLPAGLPLTAQS
                     LVPDLPTGVPSELLTRRPDVRQAEQVLLANNANIGAARAAFFPRITLTASAGVTSSEL
                     DKLFSNGTSAWTFVPQLLQPIFDAGRNKANLESAKVSRDIAVAQYEKAIQTGFREVSD
                     ALAGRATLTEQLRAQTAQLAAEQSRMMLTDMRFKHGAASSFEVLDAQRSLFAAQQAVV
                     QVQTLQVQNLVTLYKVLGGGWTEGSAQKSE"
     gene            complement(3477..3788)
                     /locus_tag="PKX82_06235"
     CDS             complement(3477..3788)
                     /locus_tag="PKX82_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51885.1"
                     /translation="MNPSRTLKVFAILLGVYGLAVLPGLFVPGYLDSPIGVVVAIPYI
                     SIYLFDMVGIPGLLQHGGMCGWGWCAPTVFRWALLAAVWLGLAWLTAKAIAAAFGRSE
                     Q"
     gene            3960..4712
                     /locus_tag="PKX82_06240"
     CDS             3960..4712
                     /locus_tag="PKX82_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51886.1"
                     /translation="MSTLHAELHRLYLLDAPGSAPDTAEPCLVASDGTVRAMVLELAR
                     PAEWGILSAVWRGVQTDLELPAPAIAVSGVDAYQLWFSLSVPVPAPQAHAFLEALRVR
                     YLSTVAPARISLLPATDRSEPTETLHARLIPALQKETGMWSAFVTPDLASIFSDEPWL
                     DLPPNPDAQANVLSHLESIQPTIFQAVLKQISPSVSAAPTGVAPASPPQEGGLDPFQF
                     LQDVMNNPAIELSHRIAAAEALLPYSQGHKGL"
     gene            4752..5153
                     /locus_tag="PKX82_06245"
     CDS             4752..5153
                     /locus_tag="PKX82_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOF51887.1"
                     /translation="MLDELPPFLIHWLITSVSLWVASHLFRGIRFADTSSLVIAALLL
                     GLANAIVKPLLIVLTLPLTVLTLGFFLLVINALMIQLVSRLVSGFSVSGFWTAFFAAI
                     FLSVFSFVLSAFLLGGSPAHPVQMPHSGVWL"
     gene            5198..>5684
                     /locus_tag="PKX82_06250"
     CDS             5198..>5684
                     /locus_tag="PKX82_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOF51888.1"
                     /translation="MVNSDHPAVVAFAREHAQGATDRERAVALYYAVRDGFRYDPYSL
                     DLTPKGMQASTVLETGLGWCVPKATLLTAVCRAAGIPARLGYADVRNHLSTERMRQSM
                     QTDVFVWHGFSDIWLDGAWQPVHQWRDQFLPKMEWHGDKLTTDQLRIKVVANRQGYGS
                     WV"
BASE COUNT         1025 a         1850 c         1751 g         1058 t
ORIGIN      
        1 gccatcgtgg tggtggagaa cgtcgagcgc atcatgagcg aggaaggcct gccgccgctg
       61 caggccaccc gcaaggccat gggccagatc tcgggcgcca ttgtgggcgt gaccgtggta
      121 ctgattgcgg tgtttgtgcc gctggctttc ttcagcggct cggtgggcaa tatctaccgc
      181 cagttctcgg cggtgatggt cagctcgatc gccttctcgg ctttcctggc cctgtcgctc
      241 acgcccgcgc tgtgcgccac gctgctcaag cccgtggagg ccggccacgc gcatgccaag
      301 accggcttct ttggctggtt caaccgccgc ttcaacagca ccgccaaggg ctatgagagc
      361 gtggtcacac gcctgctcaa gaaggcgggc cgctacctga tcatctacgc cgccatcatc
      421 ggcgcggtcg ccgtggtgta catgcgtcta cccacgtcgt ttttgcccgg agaagaccag
      481 ggcagcatca tcgtgaacgt gcaactgccc ccgggtgcca cgcaggagcg cacactcagc
      541 gtgatgcagc aggtggaagg cttcattctc aagcaacccg aagtgcaaag catggtgggc
      601 gtgctgggct tcagcttctc tggccagggc cagaatgcgg cgctggcgtt tgtgacgctc
      661 aaagactggg cggaccgccc cggaccgggt cagtcggccg aagcgctggc gggtcgtgcc
      721 tttggtgcgc tcatgggcat ccgtgatgca ttcatcttcc cgctgagccc gccccccatc
      781 cctgagctgg gctcggcatc aggcttcacc ttccgtctgc aggaccgcgc cggcaaggga
      841 cacgatgcgc tgattggcgc gcgcaaccag ctgctgggca tggcctcgca aagcaaggta
      901 ctggcccagg tacgccccga cggcctggaa gacgcgccgc agttgcagat cgacattgac
      961 cgcgacaagg ccagcgcact gggcgtgagc tttgatgcga tcaacagcac catctccacg
     1021 gcgctgggtt cgagctacat caacgacttt cccaacgccg gtcgtctgca gcgtgtggtg
     1081 gtgcaggccg atgccacagc gcgtatgcag cccgaggacg tgttgaagct caatgccatg
     1141 aacagccagg gcaaagccgt accgctgtct gcctttgcca gcacgcgctg gatcaagggc
     1201 gccatgcaaa ccgtgcgcta caacggctac cctgccgtgc gcatcagcgg tgctgccgcc
     1261 cccggcttca gcaccggtgc ggcgatgaac gagatggaaa aactcgccgc ccagctgcct
     1321 gccggctttg gcttcgagtg gaccggtcaa tcgcgccagg aaaagctggc gggctcgcag
     1381 gccatggtgc tctatggctt tgccattctg gctgtgttcc tgtgcctggc cgcgctgtac
     1441 gagagctggt ccattccgct ggcggtgatt ctggtggtgc cgctgggtgt actgggcgtg
     1501 gtgctggcca cgctgatgcg cggctacgcc aacgatgtgt acttccaggt cggcctgatc
     1561 accatcatcg gtctgtcggc caagaacgcc attctgatca tcgagtttgc caaggacctg
     1621 caggcccagg gcaagagcgc ggtggaagcc gccctggccg cagcgcacct gcgattccgc
     1681 cccatcgtca tgacctccat ggccttcgtg ctgggcgtgc tgccactggc cattgccagt
     1741 ggtgcgggct cggccagcca gcgggccatc ggcaccggcg tgatgggcgg cagcctgctg
     1801 ggaacgatgc tcgcggtgct gttcgtgcct atcttctttg tggtggtgcg cagcgtcttc
     1861 aaaggcagcg agcgccagca ccagttcgac atggaacatg gccaccagat cggagtggaa
     1921 aaacatgagt aaatcccgca tgcaacaccc tgccctcctg cggcttgtac ccctggttgc
     1981 cgcactggca ctgggcggat gcagcttcat ccccacctac gagcgccccg cggctcctgt
     2041 cgcaccgaag ttctccgcag tggccagcca ggcccccgcc gctgcggcat ccacaccggt
     2101 acgccaggcg gcagacatgg agtggcagac cttcttcaag gatgcgcgcc tcaagcgcct
     2161 gatcgaactg gcgttgcaga acaatcggga ccttcgggta gcggtgctca acatcgagca
     2221 gacccgcgcg caattccagg tccgcaaggc cgacgagttg cccacagtca atgccggcat
     2281 tgccggttcg cgtggcccta ccactaccgg tgcgatcacc agcacctaca ccgcaggcct
     2341 gagcgtgacg gcctacgaac tggacttttt cggccgcgtg cgcgcactga gccaggcggc
     2401 gcaggcccag ttgctcggga ccgaagaagc gcgcaagacg gtgcagatgg ccctgattgc
     2461 ctcgctagcc aacacctact tgaacctgct ggcagacgat gaactgctca aggtcacccg
     2521 cgatacgctg gccacgcgcg aagagtcact gcggctgacg cgcctgaagt ttgacaacga
     2581 ggccgcttcg cagctggacc tgagccaggc ccagtccgcc ctggaaggcg ccaaggcgtc
     2641 gctggcccag cagacccggc aacgtgctct ggatgaaaac gcactggtac tgctggtggg
     2701 ccagaccctg cctgcggacc tgccagcagg cttgcccctc accgcgcaga gcctggttcc
     2761 cgatctgccc acgggcgtcc cgtccgagtt gctgacacgc cgccccgatg tgcgccaggc
     2821 cgaacaggta ctgctggcca acaacgccaa catcggcgcc gcgcgtgccg cgttctttcc
     2881 gcgcatcacg ctcacagcca gcgccggtgt gaccagcagc gaactggaca agctgttcag
     2941 caacggcacc tcggcctgga ccttcgtacc ccagttgcta cagccgatct tcgatgccgg
     3001 acgaaacaag gccaacctgg agtctgccaa ggtcagccgc gacattgcag tggcccagta
     3061 tgaaaaggcg atccagaccg gcttccggga agtgtctgat gcactggcgg gccgtgccac
     3121 gctgaccgag cagctgcgcg cccagaccgc ccaactggcc gcggaacagt cgcgcatgat
     3181 gctgaccgac atgcgtttca agcatggcgc tgccagttct ttcgaagtac tggatgcgca
     3241 gcgctcgctg tttgcagcgc agcaggccgt ggtgcaggtg cagacactgc aggtacagaa
     3301 cctggtgacg ctgtacaagg tgctcggcgg tggctggacc gagggtagcg cgcagaaatc
     3361 tgaatgaaat aggcctctag tcctcgtgga atatgcgcaa gcagctatat aaaagatagc
     3421 acttgcgcga cccatcgcgc tccccagcgt tggcgccggg ttgcagccgg ttcacttcat
     3481 tgctcgctgc ggccgaatgc agcggcaatg gcttttgccg tcaaccaggc gaggccaagc
     3541 cagaccgcgg caagcaacgc ccaacggaat accgtcggcg cacaccagcc ccagccgcac
     3601 atgccgccat gctggagcag gcccgggata ccgaccatgt cgaacaggta aatcgagatg
     3661 tacggaatcg ccaccacaac gcctatgggc gaatccaggt agcccggcac gaagagaccg
     3721 ggcagcacgg ccaggccgta cacgccgagg aggatggcga agaccttcag ggtgcgggat
     3781 gggttcattg gatgaagcgg aatgatccgg cgatggtaag tcgaatattc aaggggcgta
     3841 ttggtagacc atcgaagtca cggttccaac gaagtagggc cggtcaaccc gtgacacacc
     3901 gggtagttgg accacctcgg tgcaagaccg atactctgca caacttcgta tatttcgcca
     3961 tgagtacatt acacgctgaa ttacaccgcc tctacctctt ggacgcaccc ggcagcgccc
     4021 cggatactgc agagccctgt ctggtggctt ctgatgggac cgtgcgggcc atggttctgg
     4081 agttggcccg gcccgccgaa tggggcatcc tgtctgcagt atggcgtggg gtccagaccg
     4141 atctggagct gcccgcgccc gcgatcgctg tctctggagt ggatgcttat cagctgtggt
     4201 tctcgctgtc tgtccctgtg cccgcccccc aggcgcacgc cttcctggag gcgttgcgtg
     4261 tgcgttacct gagcacggtt gcgccagcac gcatcagcct cttgcccgcc accgatagat
     4321 cggagccaac ggaaaccctg catgccagac tgatccccgc gctgcaaaag gaaaccggca
     4381 tgtggtccgc gtttgtgacc ccggacctgg cgtccatctt ctctgacgaa ccctggctcg
     4441 acctgcctcc caacccggat gcgcaggcca acgtcctgtc gcaccttgag agcatccaac
     4501 ccacgatttt ccaagccgta ctgaagcaga tcagtccgtc tgtcagtgcg gcgcctacag
     4561 gggttgcgcc tgcatcgcct ccccaggagg gcgggctgga cccgtttcaa tttctgcaag
     4621 acgtcatgaa caatccggcc attgaactca gccatcgcat tgcagcggcc gaggcgctat
     4681 tgccctactc ccagggccac aaaggcctgt agtacattga ttcccatccc atcgattccc
     4741 cttcaacgca catgctcgac gaactccccc cgtttttgat ccactggctg atcacatcgg
     4801 tctccctgtg ggtggccagt cacctgtttc gtggcatccg gtttgcagac acctcgtccc
     4861 tggtgattgc cgcgctgctg ctgggcctgg ccaacgccat tgtcaaaccc ctgctcatcg
     4921 tgctgaccct tccgttgacg gtgctgaccc tgggattttt tctgctggtg atcaacgcgc
     4981 tgatgatcca gctggtgtcg cgcctggtca gcgggtttag cgtgtcgggc ttctggaccg
     5041 ccttctttgc cgccatcttc ctgtcggtgt tcagttttgt actgagcgcc tttctgctgg
     5101 gtggcagtcc ggcccacccg gtccagatgc cgcacagcgg ggtgtggcta tgacggcgcc
     5161 tgtgcaacct gggccagcgt ccctgcaacc gactgccatg gtgaactcag accacccagc
     5221 cgtggtggcg tttgcgcgcg aacatgcgca aggcgccacg gaccgtgagc gcgctgtagc
     5281 tctgtactac gccgtgcgcg atggtttccg ctacgacccc tacagcctgg acctgacccc
     5341 caagggcatg caggccagca cggtgctgga aaccggcctg ggctggtgtg tgcccaaggc
     5401 cacgctgctg acagcggtgt gccgcgccgc cggcattccg gcccggctgg gttacgccga
     5461 tgtgcgcaac cacctgtcca ccgaacgcat gcgccagtcg atgcagactg acgtgtttgt
     5521 gtggcatggc ttctctgata tctggctcga cggcgcgtgg cagccggtgc accagtggcg
     5581 cgaccagttc ctgccgaaga tggagtggca tggagacaag ctgaccacgg atcaactgcg
     5641 tatcaaggta gtcgccaacc ggcaaggcta cggaagctgg gtgt
//
LOCUS       DAOSJM010000138         5675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12809_length_5675_cov_4.430071, whole genome shotgun sequence.
ACCESSION   DAOSJM010000138 DAOSJM010000000
VERSION     DAOSJM010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5675
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12809_length_5675_cov_4.430071"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..941)
                     /locus_tag="PKX82_06255"
     CDS             complement(249..941)
                     /locus_tag="PKX82_06255"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOF51889.1"
                     /translation="MTDNLTAPDELLLRLRRFHSDYFPLHQQRFQDLVTDGQHPKTLF
                     IGCSDSRLVPYLLTGAGPGELFIVRNVGAFVPPYDGSHGLHGTMAAIEFAVLSLKVER
                     IIVCGHSHCGAIRVAYEGAPEEAVALKAWLKLADEALLPVQPSPEAISRTEQRAVVLQ
                     LERLMAYPMVRREVEAGTLTLHGWYYIIEDGEIHVFDAQKGDFVAASVSDHSGTGPYQ
                     PYVEYDGQILSL"
     gene            complement(938..1789)
                     /locus_tag="PKX82_06260"
     CDS             complement(938..1789)
                     /locus_tag="PKX82_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF51890.1"
                     /translation="MNAAPMSTITGCGSSTCQCAASAAELDTPVASINGITLHAPGKK
                     PDVAELRELAYGELLRQQAVKLGLLPRHTGLMAPELGDEQRKVIETMVDQSVTIPQAT
                     EEECRRYYDAHKAQFVVGQALHVRHILFAVTPGVNVHALTVHAEKALLSLLGKDVPET
                     RFAQLASELSNCPTSTQGGDLGWIGPDDCAPELANELFHQKHSSWGMGVHPRLIHTRF
                     GFHIIEVLGRRKGKELPYDEVRERVAAQLAMQSRAKALHQYMTLLAGDALVEGVELES
                     SDTPLVQ"
     gene            complement(1803..2537)
                     /gene="narI"
                     /locus_tag="PKX82_06265"
     CDS             complement(1803..2537)
                     /gene="narI"
                     /locus_tag="PKX82_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOF51891.1"
                     /translation="MSYLDHFLFGYYPYICLSIFLLGSLMRFDRDQYTWKSDSSQLLR
                     AGQLRWGSNLFHVGVLFLFFGHTFGMLTPHFLYENFISAGSKQIVAMVSGGVFGVLGF
                     LGVTILLHRRLSDVRIRTNSKTSDIVLLVLLWLQLALGLATIPMSAQHLDGSMMMKLA
                     EWAQRIVTFRTGAPELLAEAGFIFKMHMFLGMSIFLIFPFTRLVHVWSGFGAAAYLVR
                     PYQVVRSRRIGLTEQQPMSRTQTTLR"
     gene            complement(2534..3211)
                     /gene="narJ"
                     /locus_tag="PKX82_06270"
     CDS             complement(2534..3211)
                     /gene="narJ"
                     /locus_tag="PKX82_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HOF51892.1"
                     /translation="MKDNRYTLRALAQFLGYPDQALRDHLPELLAAIDKEAVVPAARR
                     DELHALAADILRLDTMEAEARYVETFDRGRATSLHMFEHVHGDSRDRGPAMVDLAQTY
                     EKAGLFLSPEELPDHLCVVLEFASTQPPKLAAEFLGEMAHILNTIFSALLKRNSPYAA
                     VVAAVLEITGQRVEAVPFEADEALDDAWQEPEAFDGCSTKGQARPGDPQPIHIVRKNT
                     SKQGVTA"
     gene            complement(3228..4760)
                     /gene="narH"
                     /locus_tag="PKX82_06275"
     CDS             complement(3228..4760)
                     /gene="narH"
                     /locus_tag="PKX82_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HOF51893.1"
                     /translation="MKVRAQIAMVLNLDKCIGCHTCSVTCKNVWTTRPGMEYAWFNNV
                     ETKPGIGYPKDWENQDKWNGGWIRKSDGSIEPRQGGKWKLLMRIFSNPNMPQIDDYYE
                     PFTFDYDHLQSAPEVKAPPTARPRSLITGKRMEKIEWGPNWEEILGGEFSKRGKDANL
                     ANFDKVQKDMVGQFENTFMMYLPRLCEHCLNPACVASCPSGSIYKREDDGIVLIDQDK
                     CRGWRMCVSGCPYKKIYYNWKSGKAEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVVL
                     YDADRIQEAASVPDEKDLYQAQLDIFLNPNDPEVIAQARKDGIPDAWMAGARNSPVYK
                     MAVEWKVALPLHPEYRTLPMVWYVPPLSPITSAANAGHIGVNGEIPDIAQMRIPVQYL
                     ANLLTAGDTAPVVKALERMLAMRAYQRSKHVDQEQNLAVLEQTGLTVAEVEDMYKIMA
                     LANYEDRFVIPTSHREYAENAFDLRGGCGFSFGNGCSDGASETSLFGGTKARTIPIKS
                     MV"
     gene            complement(4776..>5675)
                     /locus_tag="PKX82_06280"
     CDS             complement(4776..>5675)
                     /locus_tag="PKX82_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HOF51894.1"
                     /translation="VTDIDAAEVILQLAPETNGHVAVKAWEALSKITGRDHTHLAIHR
                     EDEKIRFRDVQAQPRKIISSPTWSGIESETVSYNAGYTNVHEMIPWRTLTGRQHFYLD
                     HPWMVAFGEGFSSYRPPVDLKAVVGIKDRKPNGNKEISLNFITPHQKWGIHSTYTDNL
                     IMLTLSRGGPCVWLSEDDAKEAGIADNDWIELFNTNGAIAARAVVSQRVNPGMVMMYH
                     AQEKIVNTPGAEITGTRGGIHNSVTRITTKPTHMIGGYAQFSYGFNYYGTIGTNRDEF
                     VVVRKMRKIDWLDGESATPTIQA"
BASE COUNT         1096 a         1673 c         1693 g         1213 t
ORIGIN      
        1 cagctcgtcg accgtggatt cgccgggctt gaggtatttc tcccgcagca catcttcact
       61 gatgggctgg gggggcatgt ttgcatgggt catggtgcct tgatctcgtt tcatcgccag
      121 tgtgttcctt ctagatgttc cacgccgcgc cgcctgttgg ttcacccaac tgcgacgccc
      181 agcccggaaa attgattttc tgtacttcgg gtgacaccac tttgaggtag gtcatcgacc
      241 ccctgttttc acagggacaa aatttgtccg tcgtattcca cgtaaggctg gtaggggccg
      301 gtaccactgt ggtccgacac cgatgcggcg acaaaatcgc ccttctgcgc gtcaaacaca
      361 tggatctcac cgtcttcgat gatgtagtac cagccgtgca gggtcagggt tcctgcctcg
      421 acctctctgc gcaccatggg ataggccatg aggcgctcca gttgcagtac cacggcgcgt
      481 tgctcggttc ggcttatcgc ctccggactg ggctgcacgg gtagcagcgc ctcgtcagcg
      541 agtttcaacc aggctttgag cgcgaccgcc tcttccggtg caccttcata ggccacgcga
      601 atggcaccac aatggctgtg accgcacacg atgatacgct ccaccttgag gctcaggacc
      661 gcaaattcga tggcggccat cgtgccgtgc agtccatgcg aaccatcgta gggcggaacg
      721 aatgcgccga cgttgcgaac aatgaacagt tcaccgggcc ccgctcccgt cagcaggtag
      781 ggtaccagcc gtgaatccga gcagccgata aacagcgtct tgggatgctg gccgtcagtc
      841 accagatcct ggaagcgctg ctggtgcagc ggaaaataat cggaatgaaa gcgccgcaaa
      901 cgcaatagca gttcgtcagg cgccgtcagg ttatcagtca ttgaaccagg ggggtgtcag
      961 aagactccag ctccacacct tccaccaggg catctcccgc cagcagggtc atgtactggt
     1021 gcagtgcctt ggcacgggac tgcatggcca actgggccgc aacgcgttcg cgcacctcat
     1081 cgtatggcaa ctccttgcct ttgcgacgac ccagtacctc gatgatgtga aaaccgaagc
     1141 gggtatgaat cagccgggga tgcacgccca tcccccagct ggagtgtttc tggtgaaaca
     1201 gctcgttggc cagttcgggc gcgcagtcat ccggaccgat ccagcccaga tcgccaccct
     1261 gggtactggt aggacagttg gagagttcgc tggccaactg cgcaaagcgc gtctcgggca
     1321 catccttgcc cagcaagctg agcagggctt tttcggcatg caccgtgagg gcatgtacgt
     1381 tcacccccgg agtgacggca aacaggatgt ggcgcacatg cagcgcctgc cccacgacga
     1441 actgcgcctt gtgcgcgtcg tagtagcgac ggcattcctc ttcggtcgct tgcggaatgg
     1501 tgactgactg gtccaccatg gtctcgatca ccttgcgctg ctcgtcaccc aactctggcg
     1561 ccatcaagcc ggtatgcctc ggcaacagtc ccaatttgac ggcttgctgg cgaagcagtt
     1621 cgccatacgc cagttcacgc aactctgcga catccggttt cttcccgggt gcatgcagcg
     1681 taataccgtt gatgctggct actggcgtgt ccagctccgc ggcggaagcg gcacactggc
     1741 atgtactgct gccacagcca gtgatggttg acatgggtgc tgcgttcatg gcttctccct
     1801 gtttagcgca atgtggtctg ggtgcggctc atgggctgct gctcggtcag accgatgcga
     1861 cggctacgca ccacctggta cgggcgcacc agataggctg cagcaccgaa gccgctccac
     1921 acgtgcacca gccgggtgaa cgggaagatc aggaaaatgg acatgcccag gaacatgtgc
     1981 atcttgaaga tgaaacctgc ttcagccaac aactcgggag ctcctgtgcg gaaggtcacg
     2041 atgcgctgcg cccattcggc cagcttcatc atcatgctgc catccagatg ctgtgcagac
     2101 atcggaatgg tcgccagacc gagcgctaat tgcagccaca acagcaccag cagcacgatg
     2161 tcgctggttt tggagttggt gcgaatgcgg acatcgctca ggcgacggtg cagcagaatg
     2221 gtcacgccga gaaagcccag cacgccgaac acaccacccg acaccatggc cacgatctgc
     2281 tttgagccgg cactgatgaa gttttcgtac aaaaagtggg gcgtaagcat gccaaaggtg
     2341 tggccaaaaa acaggaatag cacacccacg tggaataggt tgctacccca acgcaattgc
     2401 ccggcgcgca gcagctgcga ggaatcgctc ttccaggtgt attggtcccg gtcaaagcgc
     2461 atcaggctgc ccagcaaaaa gatgctgagg cagatatagg ggtagtaccc aaaaaggaaa
     2521 tgatcaaggt aactcatgct gttactcctt gcttggaggt gtttttacga acaatgtgga
     2581 taggctgggg gtcgcctgga cgggcctgac ccttggtgct gcaaccgtca aacgcttctg
     2641 gctcctgcca ggcatcgtcc agcgcttcgt cggcttcaaa aggtaccgct tccacgcgct
     2701 gacccgtgat ttccagcacg gccgccacca cagcagcgta cgggctgttg cgcttgagca
     2761 gggcactgaa aatggtgttc aggatatgcg ccatctcgcc caggaactct gcggccagtt
     2821 tgggcggctg cgtggaggcg aactccagca ccacgcacag atgatccggc agctcctcag
     2881 gcgacaggaa caaacccgct ttttcatagg tctgcgccag gtcgaccatg gctgggccgc
     2941 ggtcgcggga atcgccgtgc acatgctcga acatgtgcag ggatgttgcg cggccacggt
     3001 cgaaggtttc gacgtagcgc gcctcggctt ccatggtgtc cagtcgcagg atgtctgcgg
     3061 ccagtgcatg cagctcgtcg cgccgagcgg ccggcaccac agcttccttg tcaatggcgg
     3121 ccagcagttc gggcagatgg tcccgcaatg cctggtcagg gtagcccagg aattgcgcga
     3181 gtgcgcgcag ggtgtagcgg ttgtctttca tgccgttctc cttgtgctca gaccatggac
     3241 ttgatgggga tcgtccgggc cttggtgccg ccgaacaggc tcgtctccga ggccccgtcc
     3301 gagcatccat taccgaacga gaatccgcag ccaccgcgca ggtcgaaggc attctctgca
     3361 tattcgcggt gcgacgtagg aatcacgaag cggtcttcgt agttcgcaag cgccatgatt
     3421 ttgtacatat cttcgacttc cgctaccgtg aggcccgttt gctccagcac ggccaggttt
     3481 tgttcctggt ccacatgctt gctgcgctgg taggcacgca tggccagcat gcgctccagg
     3541 gctttcacca cgggagcggt gtcgccagcg gtcaacagat tggccaggta ctgcactgga
     3601 atgcgcatct gggcaatgtc cgggatctcg ccattcacgc caatatgccc ggcattcgca
     3661 gccgaggtaa tgggcgacag tggcggcaca taccagacca tgggcagcgt gcggtactcg
     3721 gggtgcagcg gcagtgccac tttccactcg acagccattt tgtagaccgg gctgttacgg
     3781 gcgccggcca tccaggcatc aggaatgcca tccttgcgcg cctgggcaat cacctcgggg
     3841 tcgttcggat tgaggaagat gtccagttgt gcctggtaca ggtccttctc atccggtacg
     3901 ctggccgctt cctgaatgcg gtctgcgtca tacagcacca cgcccaggta acggatacgg
     3961 cccacacagg tctcggagca caccgtaggc tggcccgctt caatacgtgg gtagcagaag
     4021 atgcacttct cggccttgcc ggacttccag ttgtagtaaa tcttcttgta cgggcagccc
     4081 gagacacaca tgcgccagcc gcggcacttg tcctggtcga tcagcacaat gccatcgtct
     4141 tcgcgcttgt agatcgagcc ggaggggcac gaggctacgc acgcgggatt caggcagtgc
     4201 tcgcacagac gcggcagata catcatgaac gtgttttcga actgaccgac catgtccttc
     4261 tggaccttgt cgaagttggc cagattggcg tccttgccac gcttggagaa ctcgccaccc
     4321 aggatttctt cccagttggg accccactca atcttctcca tgcgcttgcc ggtgatcagg
     4381 ctgcgcggac gtgcggtggg cggtgccttg acttcaggtg ccgactgcag atggtcgtag
     4441 tcgaaggtga acggctcgta gtagtcgtca atctgcggca tgttgggatt ggagaagatg
     4501 cgcatcagca gcttccactt tccaccctgg cggggctcga tggagccatc ggacttgcga
     4561 atccagccac cgttccactt gtcctggttt tcccagtcct tggggtaacc gataccgggc
     4621 ttggtttcca cgttgttgaa ccatgcgtat tccatgccgg gacgggtggt ccagacgttt
     4681 ttgcaggtga ctgaacaggt gtggcatccg atgcacttgt ccaggttcag caccatggcg
     4741 atttgcgcgc gtactttcat gttgtgtgct ccttcttagg cctgaatggt gggagttgca
     4801 gactcgccat cgagccagtc gatcttgcgc atcttgcgca ccacgacaaa ctcatcgcgg
     4861 ttggtgccga tggttccgta gtagttgaag ccgtagctga actgggcgta gccgccgatc
     4921 atgtgcgtgg gcttggtcgt gatacgggtc accgagttgt gaatgccgcc acgggtgccg
     4981 gtaatctcgg cgccgggggt attcacaatt ttttcctgtg cgtggtacat catgaccatg
     5041 ccagggttca cgcgctggct taccaccgca cgggctgcaa tagcgccgtt ggtgttgaac
     5101 aactcgatcc agtcgttgtc agcaatgcct gcctctttgg cgtcgtcttc actgagccac
     5161 acgcaaggac caccacgact cagcgtcagc atgatcaggt tgtccgtgta ggtggagtgg
     5221 ataccccact tctggtgcgg cgtgatgaag ttcaaggaaa tttccttgtt gccattgggc
     5281 ttgcggtcct tgataccgac gaccgccttc aagtcgaccg gcggacggta cgaactgaac
     5341 ccttcaccaa aagccaccat ccaggggtgg tcgaggtaga agtgctggcg accggtcagg
     5401 gtgcgccatg gaatcatttc atgcacattg gtgtagccgg cgttgtacga cacggtctcg
     5461 ctctcgatac cactccaggt cgggctggag atgatcttgc gcggctgggc ctgcacatcc
     5521 cggaaacgga ttttctcgtc ttcgcggtgg atggcgaggt gcgtgtggtc gcgtccggtg
     5581 atcttggaca gtgcctccca agccttgacc gccacgtggc cattggtctc gggtgccagt
     5641 tgaagaatca cttcggcagc gtcaatgtcc gtcac
//
LOCUS       DAOSJM010000139         5673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12815_length_5673_cov_3.782307, whole genome shotgun sequence.
ACCESSION   DAOSJM010000139 DAOSJM010000000
VERSION     DAOSJM010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12815_length_5673_cov_3.782307"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PKX82_06285"
     CDS             <1..502
                     /locus_tag="PKX82_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HOF51895.1"
                     /translation="AMMRTTTALTIVNAGNKENVLPGRAEATVNFRILPGDTKEGVLE
                     HMRSKVAQAAPAEQFELFALPGAVDASKVAPTDSAQYKTLNKTIREIFPDALVAPGLM
                     VAGTDSIHYGPISDHIFKFSPIRANNDDLKRFHGTNERLSTKNYLDAIRFYHRLITEM
                     AALPAQ"
     gene            620..2407
                     /locus_tag="PKX82_06290"
     CDS             620..2407
                     /locus_tag="PKX82_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009976709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOF51896.1"
                     /translation="MYDIIIKNGLFFDGTGTPGALRHLGIRDGRLARISDTPLDEAHC
                     PQVIDAAERWVMPGFVDMHTHYDAELVAAPALHESVRNGVTTVMIGSCSISMVLSEAE
                     DCSDLFTRVESVPRDFVLPLLRERKTWRTPTEYVSFLRQHPLGPNIASFLGHSDLRVA
                     VMGLDRAVDGKVQPTEDEMQRMEAWLHEALDVGLMGLSTMTNPWDKLDGDRQRSKSLP
                     STYASWKEYRRLTRVLRERGAIHQSAPNLVTKVNLVLFLADSLGWFRQRLKTTIITLM
                     DLKADPMLAKVLGPITRFVNKLGADFRWQTLPSPFETFADGIEFVVFEEFPAGQAALH
                     LIDELKRNTLFQDPVYRRQFRANVEKKFGPRVWHRDLDDAWVVECPDATLVGKSVGEI
                     ARARNENPADVFLDLLIAHGRQLRWRTVIANHNPAVVEKLVNEPATLIGFSDAGAHIR
                     NMAFYNFGLRLLKLVRDAELRGQPFMPIERAVWRLTRELADWFNVDAGRLQVGDRADV
                     VVIDPAQLDASLACYREAQLAQFEGLKRLVNAGNAVDTTIIGGRVAWHQGAFAEELGQ
                     SRVFGDYLPARNRPQTQPATEPRGATVAP"
     gene            2404..2640
                     /locus_tag="PKX82_06295"
     CDS             2404..2640
                     /locus_tag="PKX82_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HOF51897.1"
                     /translation="MTPPPPIDPCLCPLCGQPNQCANEIERATGIRQPPCWCTEARFD
                     TALLERVPSEAKGKACICPACARPTTSSCALTPG"
     gene            complement(2609..2938)
                     /locus_tag="PKX82_06300"
     CDS             complement(2609..2938)
                     /locus_tag="PKX82_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51898.1"
                     /translation="MSLQDPKDDSRFKETGFPSLSWEEEDLPTRPTPLHERPLQARID
                     SEMEVIARHHVRIALAIEKFWGHRDCVEYIQSLILNGDKDGEKRVGFKPEVVSALINL
                     ASMHKKM"
     gene            complement(2977..4422)
                     /locus_tag="PKX82_06305"
     CDS             complement(2977..4422)
                     /locus_tag="PKX82_06305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOF51899.1"
                     /translation="MALGAQNFKRAAWVLLSLLVALLALVYGVLMVYGIPQNAAGMAA
                     KGICSAAFVAGRPVQSLFAEDVLPASPVLGAIRISIDERTHSVTATFAGLVSRRAVLV
                     SQRGCVLELEPDASAKAYQPAVDGSKPWPEGDAALAPKAWGPGANAVKLQQVVDAAFV
                     GAGNPPAANARGLAVVHQGRLLLLRDAPGFAPGTGLHGWSMTKTITGMLTYKLSVDAG
                     LALDAPVVDAFPAGREPDWVAAWRRDARKTIKVADLLGMREGLATTEDYAPWGSVPQM
                     LWGAPDVASWAARHPAEVPPATRWQYLSATTNVLAAVARGRFASDAEYWAYPRKAIFD
                     PIGAKSAVLETDTAGNWVGSSYLWASVGDWARLGQLMLQDGQWGTGTQKVQVLPPGWL
                     KLASTPSMQKGEGLGYGAQSWLYGNRQEGRCKDFPGVPEDTVAMGGHWGQMVAVVPSR
                     NAVVVRLGWTFRKGQFDGCQLLTDVLAALPN"
     gene            4492..>5673
                     /locus_tag="PKX82_06310"
     CDS             4492..>5673
                     /locus_tag="PKX82_06310"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOF51900.1"
                     /translation="MPLLPHFIAPVRFTDPAMALAQVMRIYDASISHLRQAMARFVAG
                     EDVNGHIRACYPLVRIHTDTVSRTAAPEVARLSYGFVAGPGRFETTLTRPDLYREYYL
                     EQFSLLLQNHQVELEIGTSTQPIPVHFSFAEHDHVEGNMSAERRMRMRDVFDLPDLAA
                     MDDGIANGTFEAKAGEAQPLSLFTAPRVDYSLHRLRHYTGTTPDHFQNFILFTNYQFY
                     IDEFIALGRAAMADPASDYVAFVEPGNVVTRRKGLPVEAEDALGKEPPRLPQMPAYHL
                     MRADRTGITMVNIGVGPANAKNITDHIAVLRPHAWIMLGHCAGLRNTQQLGDYVLAHG
                     YVREDHVLDEELPLWVPIPALAEIQVALEQAVADVTQVQGAALKSIMRTGTVASTDNR
                     NW"
BASE COUNT         1077 a         1942 c         1652 g         1002 t
ORIGIN      
        1 cgccatgatg cgcaccacca cggcgctgac catcgtgaat gctggcaaca aggaaaacgt
       61 gctgcccggc cgcgccgaag ccacggtgaa cttccgcatc ctgccaggcg acaccaagga
      121 aggggtgctc gaacacatgc gctccaaggt ggcccaggcg gcaccggccg agcagtttga
      181 actgtttgcg ctgcccggcg cagtcgatgc ctccaaggtg gcgcccaccg actcggccca
      241 gtacaagacg ctgaacaaga ccatccgcga aatcttcccg gatgcgctgg tggcgccggg
      301 cctgatggtg gcgggcaccg actcgatcca ttacggcccg atcagcgacc acatcttcaa
      361 gttctcgccc atccgcgcaa acaacgacga cctcaaacgc ttccacggca ccaacgagcg
      421 cctgagcacc aagaactacc tggacgccat ccgcttctac caccggctga tcacggaaat
      481 ggcggcgctg ccagcgcagt agttactaca aaatagatag cattcggtgc ttattccacg
      541 ggggcaatcg ctcaattctt aacgtcacct cgggttttca cgcatccagc cagcgcccct
      601 tgtgcgtaag ctggatggca tgtacgacat catcatcaag aacgggctgt tcttcgacgg
      661 cactggcaca ccgggcgctc tgcgccacct gggcattcgc gatggccggc tggcccgcat
      721 cagcgacacg cccctggacg aggcgcattg cccgcaggtg atcgacgctg cagagcgctg
      781 ggtgatgccc ggttttgtgg acatgcatac gcactacgat gcagagctgg tggctgcacc
      841 ggccctgcac gaatcggtgc gcaacggcgt gaccaccgtg atgatcggca gctgctccat
      901 cagcatggtg ttgtccgaag cagaagactg ctcggacctg ttcacccggg tggagtcggt
      961 gccgcgcgac ttcgtgctgc ccctgctgcg cgagcgcaag acctggcgta cccccaccga
     1021 gtacgtcagc ttcctgcgcc agcatccgct ggggcccaac attgcgtcct tcctgggcca
     1081 ctccgacctg cgcgtggcgg tcatgggcct cgaccgtgcc gtcgacggca aggtgcagcc
     1141 gaccgaagac gagatgcagc gcatggaggc ctggctccat gaggcgctgg acgtgggcct
     1201 gatgggcctg tccaccatga ccaatccgtg ggacaaactc gacggcgacc gccagcgttc
     1261 caagtccctg ccctccacct atgcgtcctg gaaagaatac cggcgcctga cccgggtgct
     1321 gcgtgaacgc ggcgccattc accagagcgc gcccaacctg gtgaccaagg tgaacctggt
     1381 gctgttcctg gccgacagcc tgggctggtt ccgccagcgc ctcaagacca ccatcatcac
     1441 gctgatggac ctcaaggccg acccgatgct ggccaaggtg ctgggcccca tcacccgctt
     1501 tgtcaacaag ctgggggcag acttccgctg gcagacactg ccctcgccct ttgagacctt
     1561 tgctgacggc atcgagtttg tggtgttcga agagtttcca gccggccagg ctgccctgca
     1621 cctgatcgac gaactcaagc gcaacacgct gtttcaggac ccggtgtacc gccgccagtt
     1681 ccgcgccaat gtggaaaaga aatttggccc gcgcgtgtgg caccgcgatc tggacgacgc
     1741 ctgggtcgtc gaatgcccgg acgccacgct ggtgggcaaa tccgtaggcg agatcgcacg
     1801 tgcgcgcaac gaaaacccgg ccgatgtgtt tctggacctg ctgatcgccc atggcaggca
     1861 gctgcgctgg cgcaccgtga ttgccaacca caaccctgcg gtggtggaaa agctggtcaa
     1921 cgaaccggcc acgctgatcg ggttttccga tgcgggggcc cacatccgca acatggcgtt
     1981 ctacaacttt ggcctgcgcc tgctcaagct ggtacgcgat gccgagctgc gcggccagcc
     2041 gttcatgccc atagagcgcg cggtctggcg cctcacgcgt gagctggccg attggttcaa
     2101 tgtagatgcc ggccggctgc aggtgggcga tcgggctgat gtggtggtga ttgaccccgc
     2161 ccaactcgat gccagccttg cctgctaccg tgaagcgcaa ctggcccagt ttgaaggact
     2221 gaagcgcctg gtcaatgcag gcaatgcagt ggacaccacc atcatcggcg ggcgtgtggc
     2281 ctggcaccag ggcgcgtttg cagaggaatt gggtcagtcc cgcgtgtttg gagactacct
     2341 gccagcacgc aaccgtccac agacccagcc cgccacggag cccagaggcg ctacagtggc
     2401 gccatgacgc caccgccccc catcgacccc tgcctgtgcc ctctgtgtgg ccagcccaac
     2461 cagtgtgcca acgaaatcga acgggccact ggtatacggc agccaccctg ctggtgcacc
     2521 gaagccaggt ttgatacggc gctgctggag cgtgtgccct cagaggcgaa aggcaaggcg
     2581 tgtatctgcc cggcgtgcgc cagaccgact acatcttctt gtgcattgac gccaggttga
     2641 tcagggcgga gactacttcc ggcttgaaac cgacgcgttt ttctccgtcc ttgtcgccgt
     2701 taagaatgag gctctggatg tattccacgc agtcgcggtg accccagaat ttttcaatcg
     2761 ccagggcaat ccgcacatgg tgccgggcaa tcacttccat ctcgctgtcg atgcgtgcct
     2821 gtagaggccg ttcgtgcaag ggcgtggggc gcgtaggcag gtcttcctct tcccagctca
     2881 gtgaggggaa tccggtttcc ttgaaccgag agtcatcctt gggatcctgc aacgacatgc
     2941 tccacgaaat gcgttgatcc tgaatatacc gctgaactag ttcggcagtg cagccagtac
     3001 gtcggtgagc agttggcacc cgtcaaactg ccccttgcgg aatgtccagc cgagtcgcac
     3061 caccaccgcg tttcgcgacg gcacgaccgc caccatctgc ccccagtgcc cgcccatggc
     3121 gaccgtgtcc tctggcaccc cggggaaatc cttgcaccgc ccctcctggc ggttgccgta
     3181 cagccagctc tgggcaccgt aacccagccc ctcccccttc tgcatcgacg gtgtgctggc
     3241 cagcttgagc catcctggcg gcaacacctg cactttctgg gtacccgtgc cccattgacc
     3301 atcctgcagc atcaactgcc ccagacgggc ccagtcgccc acactggccc acaggtagga
     3361 cgaccccacc cagttacccg cagtgtcggt ttccagcacc gcagacttcg cgccaatcgg
     3421 atcgaagatc gccttgcgcg gataggccca gtactcggcg tcgctggcaa agcggccacg
     3481 ggccacggcg gccagcacat tggtcgtggc gctcaggtac tgccagcggg tggctggcgg
     3541 cacttcggct gggtggcgcg cagcccaact ggccacatct ggcgcacccc agagcatctg
     3601 cggcaccgac ccccaggggg catagtcttc ggtggtggcc aacccctcgc gcatgcccaa
     3661 caggtccgcg accttgatgg tcttgcgcgc atccctgcgc caggctgcca cccaatccgg
     3721 ttcgcgaccg gcaggaaacg cgtccaccac aggcgcatcc agcgccagcc ccgcgtcgac
     3781 cgacagtttg taggtcagca tgccggtgat ggttttggtc atggaccagc cgtgcaaacc
     3841 tgtgcccggc gcaaacccgg gtgcgtctcg caacagcagc aggcgcccct ggtggaccac
     3901 tgccaggccg cgcgcattgg cagccggcgg gttgcccgcg cccacaaagg ccgcatccac
     3961 cacttgctgc aatttcacgg cattcgcacc cggcccccaa gctttcgggg ccaatgctgc
     4021 gtccccctcc ggccacggct tgctgccatc aactgccggt tggtaagctt ttgcgctggc
     4081 gtcaggctcc agttccagaa cacatccccg ctggctgacc agcacggcac ggcgtgacac
     4141 cagtccggca aacgtggccg tcacgctgtg ggttcgctcg tcgatagaga tcctgatggc
     4201 ccccagcacc ggactggctg gcagcacgtc ctcggcaaag aggctctgca ccgggcgacc
     4261 tgccacaaag gccgcagaac aaatgccctt ggcggccatg ccggccgcgt tctgcggaat
     4321 gccatacacc atcagcacac cgtacaccag tgccagcaag gccaccagca ggctcagcag
     4381 cacccacgct gcacgtttga aattctgcgc acccagtgcc atagccaatc cttcttcagg
     4441 gaaacaggtg caaccactct accccaaggc acgccgcggt accatgtgga tatgccctta
     4501 cttccccatt tcatcgcacc cgtccgcttt acggaccctg ccatggcttt ggcccaggtc
     4561 atgcgcattt acgatgccag catcagccac ctgcgccaag ccatggcgcg gtttgtcgct
     4621 ggagaagacg tcaacggcca tatccgtgcc tgctacccac tggtacgcat ccacacggat
     4681 acggtctccc gcaccgccgc ccccgaagtt gcccgcctga gttacggttt tgtcgcggga
     4741 cccggccggt ttgaaaccac cctgacgcgc ccggatctct accgcgagta ctacctggag
     4801 cagttcagtc tgctgttgca gaaccaccag gtcgaactgg aaattggaac cagcacgcag
     4861 cccattcctg tgcacttctc atttgccgag cacgaccacg tggaaggcaa tatgagcgca
     4921 gaacgtcgca tgcgcatgcg tgatgtattc gacctgcccg atctggccgc catggacgat
     4981 ggcattgcca atggcacgtt cgaggccaag gcgggagagg cacaacccct gtcgctgttc
     5041 accgcacccc gcgtggacta ttcgctgcac cgcctgcgcc actacaccgg caccaccccg
     5101 gatcacttcc agaatttcat cctgttcacc aactaccagt tctatatcga cgagttcatt
     5161 gcgctgggcc gggccgccat ggctgacccc gccagcgact atgttgcatt cgtagaaccg
     5221 ggcaatgtcg tcacgcgccg caaggggctg cctgtggaag ccgaggatgc actgggcaaa
     5281 gagccgcccc ggttgccaca gatgccggcc taccacctga tgcgcgctga ccgcaccggc
     5341 atcaccatgg tcaacatagg tgtgggaccg gccaacgcca agaacatcac cgaccatatc
     5401 gcggtactgc gcccgcacgc ctggatcatg ctgggccatt gcgccggcct gcgcaacacc
     5461 cagcaactgg gtgactatgt gctggcccac ggctatgtgc gcgaagacca cgtgctggac
     5521 gaagagttgc cgctgtgggt gccgattccg gcactggcag aaattcaggt cgctcttgaa
     5581 caggccgtgg ccgatgtcac ccaggtccaa ggggctgcac tcaagagcat catgcgcacc
     5641 ggcaccgtgg ccagcaccga caaccgcaac tgg
//
LOCUS       DAOSJM010000140         5670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12825_length_5670_cov_4.581478, whole genome shotgun sequence.
ACCESSION   DAOSJM010000140 DAOSJM010000000
VERSION     DAOSJM010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12825_length_5670_cov_4.581478"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PKX82_06315"
     CDS             complement(<1..1361)
                     /locus_tag="PKX82_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF51901.1"
                     /translation="MNYQPQTADTFHLIAHVLRAPGQLQALAPFTEIDADLMQQVLDE
                     AGKFVGEVIAPLNRDGDETGCRFDAGKVTTPPGFRDAYQAFWQAGWPALAAATEDGGQ
                     GLPAVLEAVLYENLSAANHGWTMAPGLLHGAYECIKHHASEDLKAQYLEKVATGEWLA
                     TMCLTEPHAGSDLGLARTKAVPQADGTYRISGTKIFISGGEHDLSDNIVHLVLARLPD
                     APPGPKGLSLFLVPKFYPDGSRSAVHCERIEEKMGLHGSPTCVMRFDEASGHIVGEPG
                     KGLNAMFVMMNAARLHVALQGIGLLDAAWQKADAYSRDRRQMRAPGRGKLAAEADVIA
                     EHPAMRRILDTQRAWIDGGRLLAYRTALELDIAKHHPDAARKAAAQRWCSLVTPVIKA
                     AFTHQAFYGGSECLQVFGGHGYVREWGIEQNVRDSRVAMIYEGTNEIQAIDLLVRKIL
                     SDGG"
     gene            complement(1380..2120)
                     /locus_tag="PKX82_06320"
     CDS             complement(1380..2120)
                     /locus_tag="PKX82_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF51902.1"
                     /translation="MSQNVLSVKGLQAGYGRAEVLHSIDLHAPKGSVITVIGPNGAGK
                     STFLNALMGVLPSRGSIEFEGRAIDQLTLEERVMLGIALVPEKRELFGTMPVQDNLEL
                     GAFRQVRKGNAQWREQLDVVFQLFPRLKERRTQAAGTLSGGERQMLAVGRAMMSRPTL
                     LMLDEPSLGLAPLVVREIFQTIDALRKTGVTTLLVEQNARAALETADYGYVLEMGEIA
                     LHGPAKELATDPRVIDTYLGAAKKKQAA"
     gene            complement(2133..3929)
                     /locus_tag="PKX82_06325"
     CDS             complement(2133..3929)
                     /locus_tag="PKX82_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HOF51903.1"
                     /translation="MKFTDQLSHWVHNARIQRVAVFAFMLLYAVSPVPEFWIAQLNYI
                     ALYSLVVLGLVLLTGIGGLTSFGQAAFVGIGAYTSAYLTTQMGWSPWLTVFAGLGITA
                     VSALLVGWITLRMSGHYLPLATIAWGLSLYYLMGNMDALGKYDGMLGIPPLMLGSWEV
                     SSGRSFFVLAWSAVLLGAFAVLNLLDSRTGRAIRAMRSSTLMAEAMGVDTLQHKVGIF
                     LIAALFASISGWLFAHFQHSINPSPFGLKMGIEYLFMAVLGGAGFVWGAIGGAVATKL
                     LEDWLQVLLPKLIGTSGSYEVIVFGVVLVLVLKYLPDGMWSLVGKYLPKPNRKEDWHS
                     APALPERTKPAKGDTVLEVNSIRKEFGGLVAVNDINFSIQAGQIVGLIGPNGAGKSTT
                     FNLATGVLGLTSGTVKFRGEVISGLPSREIARRGMSRTFQHVKMIPEMTVLENVALGA
                     QLRGHDNALKSMLRLDRAEEQSLLREAARQLERIGMGHAMHELAGNLAMGPQRLLEIA
                     RALCSDPALLLLDEPAAGLRHKEKQALIQVLRQLQSEGMSILLVEHDMDLVMDVTDHI
                     VVMEFGTKLMDGTPEEVQQSPKVRAAYLGVEH"
     gene            complement(3931..4968)
                     /locus_tag="PKX82_06330"
     CDS             complement(3931..4968)
                     /locus_tag="PKX82_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF51904.1"
                     /translation="MDATIASILLLDGLTNGAVYALLGLATVLVFAVTRVIFIPQGEF
                     VAYGALTLAIMQTGKVPGTVWLLLILAVVTSLVEGVAQWRHQRNATRAIKAALKMLLP
                     SVVIAGGTAWAAPHNLHIGIQAVLSVALVTAFGPLVYRLAYQSLEAATPLVLLIVSVG
                     VHFTMTGLGLLFFGAEGFRNPTFSDLRLPLGPVVFSGQALIIFAASIALIVLLWLFFE
                     RSLYGKALRATAVNRLGARLMGISSNGAGQLTFLMAALIGALSGLLIGPTTTIFYDSG
                     FLIGLKGFVAAVIGGLSSYPLALVGALFVGTVEAFASFWASAFKEVVVFSLVLPILLW
                     RSVAGGHDEEH"
     gene            complement(5073..>5670)
                     /locus_tag="PKX82_06335"
     CDS             complement(5073..>5670)
                     /locus_tag="PKX82_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF51905.1"
                     /translation="IKIVAVERFARTDTGVTAQALKLVAANPDAILVVASGSGAAMPQ
                     LGLVERGYKGKVYQTHAAASRDLMRVGGKAVEGTYVVSGPAVIAEQLPDSHPSKKVAI
                     DFVQKYEKAVGAGLRNQFAGHSYDAQIALEKAVPLALKKAKPGTPEFRLALRDAFETM
                     GRTVFAHGVMNWSKTDHWGYTLETGVMLKVVNGEFKVE"
BASE COUNT         1218 a         1813 c         1604 g         1035 t
ORIGIN      
        1 cccccatcag acaggatctt gcgcaccagc aggtcgatcg cttgaatttc gttggtgccc
       61 tcgtaaatca tggccacgcg cgagtcccgc acattctgct cgatgcccca ctcgcgcaca
      121 tagccgtggc caccaaacac ctgcaggcac tcgctgccac cataaaacgc ctggtgggta
      181 aacgccgcct tgatcacggg cgtcaccaga ctgcaccatc gctgcgctgc ggccttgcgt
      241 gcggcatcgg gatggtgctt ggcgatgtcc aactctagcg ccgtgcgata ggccagtagg
      301 cgcccgccgt cgatccaggc gcgctgtgta tcgagaatgc ggcgcatggc agggtgttca
      361 gcaatcacat cggcctctgc ggccaacttg ccacgtcccg gcgcgcgcat ctggcgacgg
      421 tctctggagt aggcatcggc tttttgccac gcagcatcca gcaagccgat gccctgcagt
      481 gccacatgca accgtgcagc gttcatcatc acgaacatgg cgttgaggcc tttaccaggc
      541 tcacccacga tgtgccctga agcctcgtca aaacgcatca cacaggtggg gctgccatgc
      601 aagcccatct tctcttcaat gcgctcgcag tgcacggcac tgcggctgcc atctggatag
      661 aacttgggca ccagaaacaa ggacaacccc ttgggaccgg gtggagcgtc aggcaagcgg
      721 gccaggacca ggtgcacgat gttgtcactc aagtcatgct caccaccgga aatgaatatc
      781 ttggtgccac tgatgcggta ggtgccatcg gcctggggca cggccttggt ccgcgccagg
      841 cccagatcgc ttccggcgtg tggctcggtc aggcacatgg tggccaacca ttcgccagtg
      901 gccactttct ccaggtactg cgctttgagg tcctcgctgg catggtgctt gatgcactca
      961 taggcaccat gcaacaagcc cggagccatg gtccagccat ggttggccgc acttaaattc
     1021 tcatacagca ccgcctccag cacggcgggc aagccctgcc ctccatcttc cgttgcagcc
     1081 gccagggctg gccaaccggc ctgccagaag gcctggtagg catcgcgaaa gcctggcggt
     1141 gtggtgacct tgccggcatc aaatcggcat ccggtttcat cgccatcacg gttgagtggt
     1201 gcaatgacct cacctacaaa tttccccgcc tcatcgagca cctgctgcat cagatccgcg
     1261 tcgatttcgg tgaaaggtgc aagcgcctgg agctgcccgg gtgcgcgcag cacatgggca
     1321 atcaggtgaa aggtgtcagc ggtttgaggt tggtaattca tggctggaga gtggagcaat
     1381 caggcggctt gttttttctt ggcggcaccg aggtaggtgt caatcacgcg cggatcggtg
     1441 gccagttctt tggccggccc atgcagggca atttccccca tctccagcac atagccgtag
     1501 tcggccgtct ccagagcggc ccgggcgttc tgctctacca gcaacgtggt gacgccggtt
     1561 ttgcgcagtg catcgatggt ctggaaaatt tccctcacca ccagcggcgc caaaccgagg
     1621 ctgggttcgt ccagcatcag caaagtgggg cgcgacatca tcgcgcggcc cacggccagc
     1681 atctggcgct cgcctcctga cagcgtgccc gcggcctggg tgcggcgctc tttcaaacgg
     1741 gggaagagct ggaacaccac atcgagttgc tcgcgccact gcgcattgcc tttgcgcacc
     1801 tgccggaatg cgcctagctc caggttatcc tgcacaggca tggtgccaaa cagctcccgc
     1861 ttttccggca ccagcgcaat ccccagcatg acgcgctcct ccagcgtcag ctggtcaatg
     1921 gcacgcccct cgaactcgat acttccgcgc gacggcagaa cgcccatcag cgcattcagg
     1981 aaggttgact tgccggcccc gttgggacca atcacggtga tcacactgcc cttgggcgca
     2041 tgcaggtcaa tggaatgcag cacctcggca cggccataac cagcctgcag gcctttgacc
     2101 gataaaacgt tttggctcat ggtggccttc cttcaatgtt ctacacccag gtacgcagcg
     2161 cgtactttgg ggctttgctg cacttcttca ggcgtgccgt ccatcagctt ggtgccgaac
     2221 tccatcacca cgatgtggtc ggtcacgtcc atcaccaggt ccatgtcgtg ctctaccagc
     2281 aggatgctca tgccttcaga ttgcaattgg cgcagtacct ggatcagcgc ctgcttctcc
     2341 ttgtggcgca gaccggctgc gggctcatcg agcagcagca gtgccgggtc gctgcacagg
     2401 gcgcgggcga tttccagcag acgctgcggg cccatggcca ggttgcccgc cagttcatgc
     2461 atggcgtggc ccataccgat gcgctccagc tgacgcgcag cttcgcgcaa caaactttgc
     2521 tcttcggcac ggtccaggcg cagcatggac ttcagtgcgt tgtcgtgtcc gcgcagttgc
     2581 gcgcccagtg ccacgttttc cagcaccgtc atttccggaa tcatcttcac gtgctggaat
     2641 gtacggctca taccgcggcg cgcgatttcg cgtgaaggca aaccactgat gacttcaccc
     2701 cggaacttga ccgtacccga ggtcaggccc aggacgccag tggcaagatt gaacgtggtc
     2761 gacttgcctg ccccgttagg accaatcagg cccacaattt ggccagcctg gatcgagaag
     2821 ttgatgtcgt tcaccgccac cagaccgcca aactccttgc ggatactgtt gacttcgagc
     2881 accgtgtcgc ccttcgctgg cttggtgcgc tcaggcaagg ccggcgcgct gtgccagtct
     2941 tccttgcggt tgggcttggg caggtatttg cctaccagcg accacatgcc gtcaggcaag
     3001 tatttcaata ccagtaccag cacaacgccg aacacaatca cttcgtagct gccgctggta
     3061 ccaatcagct tgggcaggag cacctgcaac cagtcttcca gaagcttggt cgccacggca
     3121 ccgcctatcg caccccacac aaaacctgca ccacccaaca cggccatgaa caggtattca
     3181 atgcccatct tcaaaccaaa gggggatggg ttgatcgagt gctggaagtg cgcaaacaac
     3241 cagcctgaaa tgctggcaaa caacgcagca atcaaaaaga tgcccacctt gtgctgcaac
     3301 gtgtccaccc ccatggcctc ggccatcaag gtgctcgaac gcatggcgcg aatggcacgg
     3361 ccggtacgcg agtccagcag gttcaatacc gcaaaggcgc ccagcagcac cgcactccag
     3421 gccagtacaa agaaactgcg gccactgctg acttcccaac tgcccagcat caggggcgga
     3481 atgcccagca tgccgtcgta cttgcccaat gcatccatat tgcccatcag gtagtacagc
     3541 gacaggcccc aggcaatggt ggccaacggc aaatagtggc ccgacatgcg cagcgtgatc
     3601 cagcccacca gcagtgcact gactgcggtg atgcccaacc cggcaaatac cgtgagccac
     3661 ggtgaccagc ccatttgtgt ggtgaggtaa gcgctggtgt aggcaccaat gcctacaaat
     3721 gcagcctgac cgaacgacgt caggccaccg ataccggtga gcagcaccag ccccaaaacc
     3781 accaggctgt agagcgcaat gtagttgagc tgtgcaatcc agaattccgg caccggagac
     3841 actgcgtaga gcagcatgaa cgcaaacacg gccacgcgtt gtatgcgcgc gttgtgcacc
     3901 cagtgggaga gttgatctgt gaatttcatg tcaatgttcc tcgtcgtggc cgccagccac
     3961 cgaacgccac aacaggatgg gcagcaccag cgagaacacc accacttcct tgaacgcact
     4021 ggcccagaaa gaagcaaaag cctccaccgt gcctacaaac agcgcaccca ccagcgccag
     4081 ggggtagctg gacaagccac caatcactgc ggccacaaag cccttgagcc cgatcaaaaa
     4141 tcccgagtca taaaaaatgg tggtggtggg accaatcaac agccccgaca acgcaccaat
     4201 cagcgcggcc atcaggaacg tgagctgccc cgcgccattg ctggatatac ccatgaggcg
     4261 tgcccccaga cggttaaccg cggtagcacg cagtgccttg ccatagagcg aacgttcaaa
     4321 gaacagccac agcaacacga tgagtgcaat cgacgcggca aagatgatca gtgcctgacc
     4381 cgaaaacacg acgggcccca gaggcaaacg caggtccgaa aaagtggggt tacggaaacc
     4441 ctcggcaccg aaaaacagca agcccagacc ggtcatggtg aagtgcaccc ccaccgaaac
     4501 gatcagcaac accagaggcg tagcagcctc gagcgactgg taggccaggc ggtacaccag
     4561 cgggccaaag gcggtgacca gcgcgacgct gagcacggcc tggatgccga tgtgcaagtt
     4621 gtgtggcgcg gcccatgccg tcccaccggc gatgacgacg gaaggcaaca gcattttcag
     4681 cgccgccttg atggcgcgcg tggcattgcg ctggtgccgc cactgggcca cgccttcgac
     4741 cagactggtc accacagcca gaatcagcag cagccagacc gtacccggta ccttgccggt
     4801 ttgcatgatg gccagggtga gcgcgccgta ggcgacaaac tccccctggg gaataaagat
     4861 gacgcgcgtg acggcgaata ccagcacggt agccagaccc agcagggcgt acacagcacc
     4921 attggtcaag ccgtccagca gcaggatgct ggcgatcgtt gcgtccatgg gagaaccttt
     4981 taaaaaatca gatgggggtt gggcatgccc tgcccgcggg tggcggagga ctcttgtaca
     5041 gagccccccg cgcgaatggc taggctgagg tcttattcaa ccttgaattc gccgttgacc
     5101 accttgagca tcacgcccgt ttccagggtg taaccccagt ggtcggtctt ggaccagttc
     5161 atcacgccat gggcgaatac cgtgcggccc atggtttcaa aggcatcacg caatgcaagg
     5221 cggaattcag gggttcccgg tttggctttc ttcaaagcga ggggaacggc tttctccagc
     5281 gcaatctgtg catcgtaaga gtgtccggca aactggttgc gcaaaccagc acccaccgcc
     5341 ttctcgtact tctgcacaaa gtcaatggca accttcttgg aagggtggct gtcaggcaac
     5401 tgctctgcaa tcactgcagg acccgacacc acataagtgc cttccacggc ctttccgccc
     5461 acacgcatca ggtccctgga agcggccgcg tgggtttggt aaaccttacc tttgtaaccg
     5521 cgttccacca gacccagttg tggcatggcc gcacctgaac cggaagcaac caccagaatg
     5581 gcatcggggt tggcggccac cagcttcagc gcttgggcag tcacgcctgt gtccgtacgg
     5641 gcgaaacgct caacagccac aatcttgata
//
LOCUS       DAOSJM010000141         5648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12877_length_5648_cov_4.951189, whole genome shotgun sequence.
ACCESSION   DAOSJM010000141 DAOSJM010000000
VERSION     DAOSJM010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12877_length_5648_cov_4.951189"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PKX82_06340"
     CDS             complement(<1..466)
                     /locus_tag="PKX82_06340"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907065.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOF51906.1"
                     /translation="MSDPIVIVSAARTPMGSFQGDFSALAAHDLGGAAIKAAVERAGI
                     SPELVTEVIFGNCLMAGQGQAPARQASFKGGLPKSAGAVTLSKMCGSGMRAAMFAHDM
                     LIAGSHDVLVAGGMESMTNAPYLLPKARGGYRIGHDRIFDHMMLDGLEDAYEP"
     gene            complement(506..2065)
                     /gene="thiD"
                     /locus_tag="PKX82_06345"
     CDS             complement(506..2065)
                     /gene="thiD"
                     /locus_tag="PKX82_06345"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOF51907.1"
                     /translation="MRARSTFGTELRPVRPGVRPIVWSIAGSDSGAGAGLQADLKALQ
                     AFGVHGCTAVASITAQNSVAVTRVEPVSAELLDAQLDALAHDLPPQAIKTGLLGSVDN
                     VRVVCRWVDRLRARGLPVALVVDPVLGSTTGASFASEALVQAYVQELLPRATLVTPNR
                     AEAARLGLGVGQAYQPGQASVVITGGDDSKENGLASDWLHCPQASGWLSLPRVTTAHH
                     HGTGCTFASTAAAALASGYCVADAVVLAKMATTHALRHAYAAGQGAGPVQAWPDFAQH
                     IDNLPAFTLPGATTPLALHTQQPCALLNNPALGVYAVVDSADWVRRVLAAGIRTVQLR
                     IKDPTEPKLAAQIQDSIAIARATPGAQLFINDHWQLALQHGAYGVHLGQEDLETVDLD
                     ALRNAGIRLGLSTHNYWEVARAWALRPSYIACGPVYATKSKDMPWIPQGLDNLRYWAG
                     VLPLPVVGIAGIDASNVAAVAATGAASAAVISAITQVADPVQACRALQQGFAGNGFFD
                     STRLLSQLTFT"
     gene            complement(2103..2996)
                     /locus_tag="PKX82_06350"
     CDS             complement(2103..2996)
                     /locus_tag="PKX82_06350"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HOF51908.1"
                     /translation="MSEQQKDPVVVGSAQVKEEPAQRAGDTEQSPLPPGRSGTSPWHI
                     GGRTLTSRFFLGTASYPSPQVLQEAIAASGAQVVTMGLRRQLAHGGQADDFFTLVQAT
                     GAHLLPNTAGCHTAREAVTLAHMARELFDTHWIKLEVVGDAHTLQPDPFELLTATAQL
                     VKDGFEVFPYCMDDLVSCQRLLDAGCNILMPWGAPIGSGQGLVNPGALRTLRARLPGV
                     PLIVDAGIGSPVDAVQAMELGLDAVLLNSAVAHAVDPVRMARAFKLGIEAGRLAFEAG
                     IMARQDMAVASTPVAGHPILL"
     gene            complement(3001..3228)
                     /gene="thiS"
                     /locus_tag="PKX82_06355"
     CDS             complement(3001..3228)
                     /gene="thiS"
                     /locus_tag="PKX82_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOF51909.1"
                     /translation="MSTPDFISESFTVVVNGQPQTTTARTLQDWVDAQSVQPAALATA
                     VNGEFVPRDLRAQRLLCVGDLIVTFQPIQGG"
     gene            complement(3225..4265)
                     /locus_tag="PKX82_06360"
     CDS             complement(3225..4265)
                     /locus_tag="PKX82_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF51910.1"
                     /translation="MHVGIAGAGLLGRLLALQLSAEGHRVEVFDPASGPGPRETSGTQ
                     AAAWTAAGMLSPVAELDRSNEDVFHLGLRSCALWPGIAQALGVPQVCHFDGSLLLAHR
                     SDAGAAQRLVGLLQAKAGAAYLPQALQAEALAQLEPGIHGPSLAWLLPCEGYIEVHNT
                     LQALHAQGTRQGVLWHWGRTVAAVEPGALVLSNSVRAEFDHVFDVRGVGAKPALPMRG
                     VRGEVFLLHAPGASLRRPVRLMHPRHSVYIVPRPDDCLVVGASEIESEDRSPVSLRST
                     LELLSAAHSAIPALAEARVVHTETNLRPALPDNLPVLKHSAGLTCINGLYRHGWLLAP
                     ALVEQALKELSL"
     gene            complement(4288..>5648)
                     /gene="thiC"
                     /locus_tag="PKX82_06365"
     CDS             complement(4288..>5648)
                     /gene="thiC"
                     /locus_tag="PKX82_06365"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOF51911.1"
                     /translation="GNSFGARIPKVVTPEFVRDEVARGRAIIPANINHTELEPMAIGR
                     NFLVKINANIGNSAVTSSIEEEVEKLAWSIRWGADTVMDLSTGKNIHTTRDWIVRNSP
                     VPIGTVPIYQALEKVGGIAEDLTWDIFRDTLIEQAEQGVDYFTVHAGVRLPFIHLTAN
                     RVTGIVSRGGSIMAKWCIAHHKENFLYTHFDEMTEIMKAYDVSYSLGDGLRPGSGADA
                     NDEAQFAELRTLGELTHKAWQQDVQVMIEGPGHVPMHMVQANMTEQLKHCGEAPFYTL
                     GPLTTDIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPDREDVKVGIITYKIA
                     AHVADVAKGHPGVRARDDALSKARFEFRWMDQFNLSLDPETAKQFHDETLPAEGAKVA
                     HFCSMCGPKFCSMKITQEVRDYAKNLGVSEEQALQSGMEVMSDEFKRVGGEIYIPIQP
                     AQ"
BASE COUNT          967 a         1844 c         1848 g          989 t
ORIGIN      
        1 cgggctcgta ggcgtcttcc aggccgtcga gcatcatgtg gtcgaagatg cggtcatggc
       61 cgatgcggta gccgccgcgc gccttgggca gcaggtaggg cgcgttggtc atgctttcca
      121 tgccgccggc caccagcacg tcgtggctgc cggcgatcag catgtcgtgc gcaaacatgg
      181 ctgcacgcat gccggagcca cacatcttgg agagcgtgac cgcccctgcg ctcttgggca
      241 ggccaccctt gaacgacgcc tggcgtgccg gtgcctggcc ctgacctgcc atcaggcagt
      301 tgccaaaaat cacttccgtc accagctccg gactgatgcc ggcgcgctcg accgcggcct
      361 tgatggcggc gccacccagg tcatgggcgg ccagcgcaga aaagtcaccc tgaaaactgc
      421 ccatgggggt acgggcggcg ctaacgatca cgatggggtc tgacatggtt ttcctcgttt
      481 cagatggaga taaagcaatt gacgtttacg taaacgtcaa ttgtgacaga agacgcgtgg
      541 aatcgaaaaa gccgttgcca gcaaagccct gctggagtgc gcggcatgcc tgcacaggat
      601 cagcgacttg cgtaatcgcc gaaatgaccg ccgcactggc cgcgcccgtg gcggccaccg
      661 ccgccacgtt gctggcatca atgcccgcaa tgccgaccac cggcaacggc agcacgccgg
      721 cccagtaacg caggttgtcc aagccctgcg gaatccaggg catgtctttg ctctttgtgg
      781 catagacggg gccgcaggcg atgtagctgg gacgcagggc ccaggcgcgg gccacctccc
      841 agtagttgtg ggtgcttagc cctaagcgga taccggcatt tctcagggcg tccaagtcca
      901 cggtttccag atcttcctgg cccagatgca cgccataggc gccgtgctgc aatgccaatt
      961 gccagtggtc gttgatgaaa agttgtgctc ccggggtggc gcgcgcaatg gcgatgctgt
     1021 cctgaatttg cgcagccaac ttaggttctg tggggtcctt gatgcgcagc tgcacggtgc
     1081 ggatgccagc cgccaacacg cgacgcaccc agtcggcgct gtccaccacg gcgtagacac
     1141 cgagagcggg gttattgagc agcgcacagg gctgctgcgt gtgcagggcc agaggtgttg
     1201 tggcacctgg cagcgtgaag gccggcaagt tgtcgatatg ctgcgcaaag tccggccagg
     1261 cttgcaccgg gcccgctccc tgccctgcgg cataggcgtg gcgcagcgcg tgcgtagtgg
     1321 ccatcttggc cagcaccacg gcatcggcca cacagtagcc gctggccagg gcggcggcgg
     1381 cggtggatgc gaaggtgcag cctgtgccgt ggtggtgggc tgtcgtcacg cgcggcaggc
     1441 tgagccagcc actggcttgc ggacaatgca accagtcgct ggccagccca ttttctttgg
     1501 aatcgtctcc tccggtgatc accacagacg cctgacctgg ttgatacgct tgccccacac
     1561 ccagccccag gcgcgcagcc tccgcacggt tgggcgtgac cagcgtggcg cgtggcagca
     1621 gctcctgcac ataggcttgc accaacgctt cacttgcaaa actggcaccg gtcgtggaac
     1681 ccagcaccgg gtccaccacc agcgccaccg gcaggcctcg cgcacgcaag cggtccaccc
     1741 atcggcagac cacgcgcacg ttgtccacgc taccgagcaa cccggtcttg atggcctgtg
     1801 gcggcaggtc gtgcgcgagg gcatccagtt gcgcatccag cagctcggcg ctgaccggtt
     1861 ccacgcgcgt cacagccacc gagttctgcg cggtgatgga cgccaccgcc gtgcagccgt
     1921 gcacaccaaa ggcctgcagg gctttgagat cggcctgcag gccggcaccg gcgcccgagt
     1981 cggagccggc aatgctccag acgatggggc gtacaccagg ccttaccggg cgcagttcag
     2041 tgccaaatgt gcttctggcc ctcataggta ttgcggtatt agctattagt ttcatagcaa
     2101 actcataaca aaatgggatg ccccgccacc ggcgtggacg ccaccgccat gtcctggcgg
     2161 gccatgatgc cggcctcaaa cgccaggcga ccggcctcaa tgccgagctt gaacgcgcgc
     2221 gccatgcgca ccgggtccac cgcgtgggcc acggcgctgt tgagcagcac ggcgtccagc
     2281 cccagctcca tggcctgcac tgcatccacc ggcgagccga tgccggcgtc cacgatgagc
     2341 ggcacaccgg gcagacgcgc gcgcaaagtg cgcagcgcgc cagggttgac cagcccctgg
     2401 cccgagccaa ttggcgcacc ccagggcatc aggatgttgc agcccgcgtc cagcaggcgc
     2461 tggcaactca ccagatcgtc catgcagtag ggaaagactt cgaagccgtc tttgaccaac
     2521 tgcgcagtag cggtcagtag ctcgaacggg tccggctgca gcgtgtgggc gtcgcccacc
     2581 acttcgagct tgatccagtg cgtgtcaaac agctcgcgtg ccatgtgggc cagcgtgaca
     2641 gcctcgcgcg ccgtgtggca gcccgcggtg ttgggcagca ggtgggcgcc ggtggcttgc
     2701 accagcgtaa agaaatcgtc cgcctgacca ccgtgcgcga gctggcggcg cagccccatg
     2761 gtgaccacct gcgcgccgga agcggctatc gcctcctgca acacctgcgg cgacgggtag
     2821 ctggcggtac cgagaaagaa acggctggtg agcgtgcgtc cgccgatgtg ccacgggctt
     2881 gtgcctgagc gccccggcgg cagcgggctc tgctccgtgt cccccgcccg ctgtgcgggc
     2941 tcctccttta cctgcgcaga acccactacc accgggtctt tctgttgctc ggacatgggt
     3001 tcagcctccc tggatgggtt gaaaggtcac gatcagatcg ccgacgcaga gcaggcgttg
     3061 cgcgcgcagg tcgcgcggca caaactcgcc attgaccgcc gtggccagcg cggcgggttg
     3121 cacgctctgc gcatccaccc aatcttgcaa ggtgcgggcg gtggtggttt gcggctggcc
     3181 attcacaacc acggtgaagg actcggaaat gaaatccggt gtactcatag cgataactcc
     3241 ttgagggcct gctccaccag cgcgggcgcc agcagccagc catggcggta caggccgtta
     3301 atgcaggtca ggcccgcgct gtgcttcagc accggcaggt tgtcgggcag ggccgggcgc
     3361 agattggtct cggtatgcac cacgcgtgcc tcggccagtg cgggaatggc gctgtgcgca
     3421 gcactcagca attccagtgt gctgcgtagc gacacgggcg aacggtcctc actctcgatc
     3481 tcgctggcgc ccaccaccag gcagtcgtcg ggccggggca cgatgtagac gctgtggcgc
     3541 gggtgcatca ggcgcaccgg acgcctcaag ctggcgccgg gcgcatgcag caaaaatact
     3601 tcgccccgca caccacgcat tggcaacgcg ggcttggcgc ccacaccgcg tacgtcaaac
     3661 acgtggtcaa actcagcccg aacggaattg gagagcacca aggcccccgg ctccaccgcc
     3721 gccaccgtgc gaccccagtg ccacagcacc ccctgccggg tgccttgcgc atgcagggcc
     3781 tgcagggtgt tgtgtacctc gatatagccc tcgcagggca ggagccaggc cagcgatggt
     3841 ccgtggatac cgggctccag ctgcgccagt gcttcggcct ggagtgcctg gggcaggtaa
     3901 gcggcaccgg ccttggcctg cagcaggccg accagccgct gggccgcacc ggcatcactg
     3961 cggtgggcca gcagcagact gccatcaaag tgacagacct ggggcacacc cagcgcctgc
     4021 gcaatgccgg gccacagcgc gcaggagcgc aggcccaggt ggaacacgtc ctcgttggag
     4081 cggtccagtt cggccacggg actgagcatg cccgcagccg tccaggcagc ggcctgggtt
     4141 cctgaagttt cgcggggccc cggaccactg gcggggtcaa agacttccac gcggtgccct
     4201 tccgcgctaa gctggagcgc cagcaatcgg ccgagcaagc cggcccctgc aatacccaca
     4261 tgcatggtgt tttcctcagg acggcggtta ttgcgcgggc tggatgggga tgtagatttc
     4321 cccgcccacc cgtttgaact cgtcggacat cacctccatg cccgactgca gcgcctgctc
     4381 ctcgctcacg cccaggttct tggcgtagtc gcgcacctct tgcgtgatct tcatgctgca
     4441 gaacttgggg ccgcacatgg agcagaagtg cgccaccttg gcgccttcgg cgggcagggt
     4501 ttcgtcgtgg aactgcttgg cggtttccgg gtccagcgac aggttgaact ggtccatcca
     4561 gcgaaactcg aagcgcgcct tggacagcgc atcgtcgcgc gcccgcacac ccgggtggcc
     4621 cttggccacg tcggccacat gggcggcaat cttgtaggtg atgatgccca ccttcacatc
     4681 ttcgcggtcc ggcagaccca ggtgttcctt gggggtgacg tagcacagca tggcggttcc
     4741 gaaccagccg atcatggccg cgccaatgcc gctggtgatg tggtcgtagc ccggtgcaat
     4801 gtcggtggtc agggggccca gggtgtagaa cggggcttcg ccgcagtgct tgagctgctc
     4861 ggtcatgttg gcctgcacca tgtgcatggg cacgtggccg gggccttcga tcatcacctg
     4921 cacgtcctgc tgccacgctt tgtgcgtgag ctcgcccagc gtgcgcagct cggcaaattg
     4981 cgcttcgtcg ttcgcgtccg caccgctgcc ggggcgcagg ccatcgccca gcgagtagct
     5041 cacgtcatac gccttcatga tttcggtcat ctcgtcgaag tgcgtgtaca agaagttttc
     5101 tttgtggtgg gcaatgcacc acttggccat gatggagccg ccgcgcgaca cgataccggt
     5161 cacgcgattc gccgtcaggt ggatgaaggg caggcgcacg ccggcatgca ccgtgaaata
     5221 gtccacgccc tgctcggcct gctcgatcag cgtgtcgcgg aagatgtccc aggtcaggtc
     5281 ttcggcaatg ccgcccactt tttccagcgc ctggtaaatc ggcacggtgc cgatgggcac
     5341 cgggctattg cgcacgatcc agtcgcgtgt ggtgtggatg tttttgccgg tcgacaagtc
     5401 catcactgtg tcggcgcccc agcggatgga ccaggccagt ttttccactt cttcctcgat
     5461 gctggaggtg acggccgaat tgccaatgtt ggcgttgatc ttgaccagga agttgcgccc
     5521 aatggccatg ggctccagct cggtgtggtt gatgttggcg gggatgatgg cgcggccgcg
     5581 ggcgacctcg tcgcggacaa actcgggggt gaccaccttg gggatgcggg cgccaaagct
     5641 gttgccgg
//
LOCUS       DAOSJM010000142         5606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_12984_length_5606_cov_4.849216, whole genome shotgun sequence.
ACCESSION   DAOSJM010000142 DAOSJM010000000
VERSION     DAOSJM010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12984_length_5606_cov_4.849216"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="PKX82_06370"
     CDS             <1..428
                     /locus_tag="PKX82_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOF51912.1"
                     /translation="TKMGQAAIFETRGNDDCHVILRGGKTTNYSKADVDAACALLKAA
                     GLREQVMIDVSHANSSKQHARQIVVAGEVAAQIAAGDRRITGIMIESHLNEGRQDILP
                     GVPLKHGVSVTDACISMAQTVPVLQELAAAVRTRRASQQ"
     gene            492..1208
                     /locus_tag="PKX82_06375"
     CDS             492..1208
                     /locus_tag="PKX82_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="HOF51913.1"
                     /translation="MKLCILENDILEGELAQSYGGFGALFFRLFESVGVDWHMEVFNT
                     MRGEYPASFDDYDAVLLTGSRADSFSDEPWVVELRRQVTQLLKEHKKMVGVCFGHQLI
                     ALCLGAPVGRAPQGWGTGRMTYEWHRPDLPHSQNRTHISLLASHQDQVLALPPGAKLV
                     ASNDFCPVASYTVGDAVFCIQPHPEFSPDISAHLLDKRRHLLGEAHYTASRLSLADGH
                     EGEHIGRMVAAFLKGEPPVA"
     gene            complement(1205..1696)
                     /locus_tag="PKX82_06380"
     CDS             complement(1205..1696)
                     /locus_tag="PKX82_06380"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOF51914.1"
                     /translation="MTHEPRLTTALRALLASQRVAALGTLDAQGLPFVSMVPFAVEPL
                     QGSMVLHVSGLALHTRNLQAAPQVSLMVMEAEKAGAPVHALARVTLVGVAGVLPSDTP
                     AWRDARNAYLERFPEAEPMTQLGDFMFVAIRVDGARQVAGFGAARSLDAQDMAQVLQS
                     TGS"
     gene            1967..2431
                     /locus_tag="PKX82_06385"
     CDS             1967..2431
                     /locus_tag="PKX82_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOF51915.1"
                     /translation="MELDRYDRHILEILQQEGRIANQDLADRIGLSPSPCLRRVRALE
                     ESGLIRGYRALLDARKLGLSLMALVYISMDRHTPERFENFEAAIGLLPEVLECLLITG
                     QDADYQLKVVVRDMDHYQALLLNTLTRIEGVTGVHSSFVLRRVLDKTALAVS"
     gene            2446..3210
                     /locus_tag="PKX82_06390"
     CDS             2446..3210
                     /locus_tag="PKX82_06390"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOF51916.1"
                     /translation="MCLIAWSWQPASATPLLLLSNRDEFYHRPALPLHWWEPAPGGVE
                     ILAGRDLQAGGTWLGVSRTGRLAAITNYRTPVPARDNTPSRGELVAGFLQGSQSAMEY
                     LQALSDRAQDYNPFNLLVFDGEQLLGLESRQARIVTLKPGMGAVSNADFHTPWPKLTR
                     LQTGLHTHVETGNTRADQVFALLHDATLANDDELPQTGVPVALERALSATFIATPGYG
                     TRACSVVHIHQHAVEFTEQSFGPAGLLDSRQTCFSY"
     gene            complement(3207..4910)
                     /locus_tag="PKX82_06395"
     CDS             complement(3207..4910)
                     /locus_tag="PKX82_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HOF51917.1"
                     /translation="MLIETLGAARDMGRLSEIAGVLIRHGFGDTVRRLGLADRLARAG
                     HAIQMDHAADLARLEPPVQVRLAMEELGPTFVKLGQILAGRADIFGPEWIAEFEKLHS
                     HVPPVSLDVLLPQLQQDLGAPPHEVFARFDVEPLAAASIAQVHRAQLQDGTEVIVKIR
                     RPGIDAVIEADLRLLGHLAALVEAEIPALKPYRPQQLVREFAKSLRRELDLAAECRNA
                     EHIAANMAALSFIVIPKVHWAYTGQRVNVQDYVGGVAGDALAQLTTESGFDRTLLAQR
                     GAQAVLKMIVQDGIFHADPHPGNVFYLSDNRIAFIDFGMVGRLSSRRREELLQLLLGL
                     VERQPQTVADVLLDWTGDDHSVNLSQLETEIEAFVDQYHGTPLAELSLGQMLTDVTAI
                     LRDHHLGLPSDLALLIKAFISLEGMGRGLDPNFHMATEATPMLRKVVRARYRPELVAK
                     RAWQTVRRTLAMVEQLPHDISSLLRNARRGRVHVGIELAHLKRVGNQIDRAANRLSMA
                     LVIAALIIGSSIVMNVQGGPTLFGLPMFGFLGFVGAVLGGFWLVRSIWRSGRSDEGDE
                     P"
     gene            complement(4967..5230)
                     /gene="minE"
                     /locus_tag="PKX82_06400"
     CDS             complement(4967..5230)
                     /gene="minE"
                     /locus_tag="PKX82_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOF51918.1"
                     /translation="MASLLSFFLGEKKSTASVAKERLSILLAHERAGRNASAPDYLPE
                     LQRELVAVISKYVKINPEDIKVIAGRQDNLETLEVKIELPDGR"
     gene            complement(5234..>5606)
                     /locus_tag="PKX82_06405"
     CDS             complement(5234..>5606)
                     /locus_tag="PKX82_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025606.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOF51919.1"
                     /translation="SVRDSDRILGMLASKTKRAMEGGGAIKEHLLITRYNPSRVNTGQ
                     MLSIDDIKDILRIDLIGVIPESESVLQASNQGVPAVHIQGSDVSEAYKDVIERFLGKD
                     VPMRFTDAPKQGLLKRLFGGK"
BASE COUNT         1091 a         1749 c         1765 g         1001 t
ORIGIN      
        1 tgaccaagat gggccaggcg gcgatcttcg agacccgcgg caacgacgac tgccatgtga
       61 ttctgcgcgg cggcaaaacc accaactact ccaaggccga tgtggatgcg gcttgcgccc
      121 tgctcaaggc cgccggcctg cgcgagcagg tgatgattga cgtgagccac gccaacagca
      181 gcaaacagca cgcacgccag atcgtggtgg cgggtgaagt ggcggcccag attgcagcgg
      241 gagaccggcg catcaccggc atcatgatcg agagccacct caacgaaggc cggcaggaca
      301 tcctgcccgg tgtgccgcta aagcacggcg tatcggtcac cgacgcctgc atcagcatgg
      361 cgcagactgt gccagtgctg caggaactgg cagcggcggt gcggacacgc cgggccagtc
      421 agcagtaatt gaacgtattt agcctctagc ccccgtggaa tatgcgtaag tagctattaa
      481 atgtgtagca aatgaaactc tgcattcttg aaaacgacat ccttgaaggc gaactggcgc
      541 agtcgtatgg cgggtttggg gccctgtttt tccggctgtt cgagagtgtg ggcgtggact
      601 ggcacatgga ggtgttcaac accatgcgcg gcgagtaccc ggcgtcgttc gacgactatg
      661 acgcggtgct gctgactggc agccgggccg attcgttttc cgacgagccc tgggtggtgg
      721 agctgcgccg gcaggttacc cagctgctga aagagcataa gaagatggtg ggtgtgtgct
      781 ttggccacca gctgattgcg ctgtgcctgg gcgcacccgt gggccgcgcg ccgcagggct
      841 ggggtaccgg acgcatgacc tacgagtggc accgcccgga cctgccgcac tcgcaaaacc
      901 gcacccacat ctcgctgctg gccagccacc aggaccaggt gctggcgctg ccacccggcg
      961 ccaaactggt ggccagcaac gacttctgcc cggtggcctc gtacaccgtg ggcgacgcgg
     1021 tgttctgcat ccagccgcat ccggagttca gcccggacat cagcgcccat ctgctggaca
     1081 agcgccgcca tctgctgggc gaagcccatt acacggccag ccgcctgagt cttgccgacg
     1141 gccacgaagg cgagcacatt ggccgcatgg tggcggcctt tctgaaaggc gagccgccgg
     1201 tcgcttaaga cccggtggac tgcagtacct gcgccatgtc ctgcgcatcc agcgatcggg
     1261 cagcgccaaa accggccacc tggcgtgctc cgtcgacacg gatcgccaca aacatgaagt
     1321 cgcccaactg ggtcatgggc tccgcctcgg ggaagcgctc caggtaggcg ttgcgcgcat
     1381 cgcgccaggc gggggtgtca gagggcagca ccccggcgac gccgaccagt gtcacccgcg
     1441 ccagcgcgtg caccggcgca ccggctttct cggcttccat caccatcagc gacacctgtg
     1501 gcgcggcctg caggttgcgt gtgtgcagtg ccagtccgct gacatggagg accatgctgc
     1561 cctgcagcgg ctccaccgcg aacggcacca tggaaacaaa cggcagaccc tgtgcatcca
     1621 gggtgcctag ggcggcgacg cgctgcgacg ccagcagggc acgcaaggcg gtggtcaatc
     1681 ggggttcatg ggtcatgggc acaagtatgg cgcagcacga tgctgcctga ccccctaaaa
     1741 agacatggcc ccggagcagg taatgcgccg gggccattgt ttttgcaatg cagtccctcg
     1801 tgaggccaga tcgcgcaacc tgggccggta agcctggttg ctgtgtgctg cgataacaga
     1861 ggactccagt gtaggccagg gggcgaggca gatgattgcg ttgttgctct gtaaatcttg
     1921 atttttggca tactctgcca attaactact atttcttggc agatctatgg aactggaccg
     1981 ctacgaccgg cacatactgg aaatattgca gcaggagggc cgcatcgcca accaggacct
     2041 ggccgaccgc attggcctgt cgccctcgcc ctgcctgcgc cgggtgcggg cgcttgaaga
     2101 gtcgggtctg atccgcggct accgggcttt gctggacgcc aggaagctgg ggctgagcct
     2161 gatggcgctg gtctatatct ccatggatcg ccacacaccg gagcgtttcg aaaattttga
     2221 ggcggccatc gggctgctgc ccgaggtgct ggagtgcctg ctgatcaccg gccaggacgc
     2281 cgactaccag ctcaaggtgg tggtgcgcga tatggaccac taccaggcgt tgctgctcaa
     2341 cacgctcaca cgcattgagg gcgtcaccgg ggtgcattcc agctttgtgc tgcgccgggt
     2401 gctggacaag acggcgctgg cagtgtcctg aacgagaatg acgccatgtg tctgatcgcc
     2461 tggagctggc aacctgccag tgccacccct ttgctgctgc tgtccaaccg cgacgagttt
     2521 taccatcgcc cggcgttgcc gctgcattgg tgggaacctg cgccgggagg agttgaaata
     2581 ctggcaggca gggacctgca agccggcggc acctggctgg gggtgagccg cacgggtcgc
     2641 ctggcggcga tcaccaacta ccgcaccccc gtgcctgcgc gcgacaacac accatcacgc
     2701 ggggaactgg tggccgggtt tctgcaaggt tcccagagcg ccatggagta cctgcaggcg
     2761 ttaagcgaca gggcccagga ttacaacccc ttcaacctgc tggtgttcga tggcgagcag
     2821 ctgctcgggc tggagagccg gcaagcccgc atcgtgaccc tgaagcccgg catgggcgca
     2881 gtatccaacg cagactttca caccccctgg cccaagctga cgcggctgca aacgggcctg
     2941 cacacccatg tagagactgg caacacacgc gctgaccagg tgtttgcact gctgcacgat
     3001 gcgaccttgg ccaacgatga cgaactgccg caaaccggag tgcctgtggc gctggagcgc
     3061 gccctgtcag ccaccttcat tgccacgccg ggttatggca cacgggcctg cagcgtggtg
     3121 catatccacc agcacgcggt ggaattcacc gagcagagct ttggacctgc gggcctgctg
     3181 gacagccggc agacctgctt cagttactaa ggctcatcac cctcatcact gcggccgctg
     3241 cgccagatgg agcgcaccag ccagaacccg cccagcaccg cgcccacaaa gcccagaaag
     3301 ccgaacatgg gtaagccaaa cagcgttggg ccaccctgaa cgttcatcac gatggaggat
     3361 ccgatgatga gtgcggcgat caccagcgcc atcgacaggc ggttggcggc acggtcaatc
     3421 tggttgccca cgcgcttgag gtgggccagc tcgataccca catgcacccg cccgcggcgc
     3481 gcattgcgca gcagggaaga aatgtcatgc ggcagctgct ccaccatggc cagcgtgcgc
     3541 cgaacggttt gccatgcgcg ctttgccacc agttcggggc ggtagcgcgc acgcaccacc
     3601 ttgcgcagca tgggcgtggc ctcggtcgcc atatggaaat tggggtccag cccccgtccc
     3661 atgccttcga gcgagatgaa ggccttgatc agcagggcca ggtcggacgg caatcccaga
     3721 tggtggtcgc gcaggatggc ggtgacatcg gtcagcatct gccccaggct gagttcggcc
     3781 agcggtgtgc cgtggtactg gtcgacaaac gcctcgatct cggtttccag ctgggagagg
     3841 ttcacactgt ggtcgtcgcc agtccagtcc agcaacacat cggccacggt ctgcggctgt
     3901 cgctcgacca accccagcag cagttgcagc agctcttcgc gccggcggct ggacaggcgg
     3961 ccgaccatgc caaagtcgat aaaggcaatg cggttatcac tgaggtagaa cacattgccc
     4021 gggtgcggat cggcatggaa gatgccgtcc tgcacaatca tcttgagcac ggcctgggca
     4081 ccccgctggg ccagcagggt acggtcaaac ccagactctg tggtcaactg ggctaatgca
     4141 tcgccagcca caccccccac atagtcctgc acattgacgc gctggccggt gtaggcccag
     4201 tgcactttgg gaatcacgat gaaggacaag gctgccatgt tggccgcaat gtgctcggcg
     4261 ttacggcact cagcggccag atcgagttcc cggcgcagcg acttggcaaa ctcccgcacc
     4321 agttgttgcg ggcggtaggg tttgagtgca ggtatttctg cctcgaccag cgcagccaaa
     4381 tggcccagca ggcgcagatc ggcttcaatg actgcatcaa ttccggggcg acggattttg
     4441 acaatgacct cggtcccgtc ctggagttgt gcccggtgca cctgggcaat ggaggccgct
     4501 gccagcggct ccacatcaaa acgtgcaaac acctcatgcg gcggcgcgcc cagatcctgt
     4561 tgcaactggg gcaacagcac gtccagcgaa acgggcggaa catggctgtg cagcttctca
     4621 aactcggcaa tccattcggg tccgaagatg tcggcacggc ccgcaagaat ctggcccagt
     4681 ttgacaaagg tcggacccag ttcttccatt gccaggcgca cctgcaccgg aggttccagg
     4741 cgggccagat cggccgcatg gtccatctgg atggcatggc ctgcgcgggc cagccggtct
     4801 gccagaccga gccggcgcac cgtatcgcca aaaccatggc ggatcagtac cccagcaatc
     4861 tcgctgaggc gacccatatc acgcgcggca cccaatgttt caatcagcat gcgcggatga
     4921 tagccccgcg cctttcgaac gcgaagaccg gaaaacggac tatgccctag cggccgtccg
     4981 gcagttcgat cttgacttcc agcgtctcca ggttgtcctg gcgcccggca atgaccttga
     5041 tgtcttcggg attgattttg acgtacttgc tgatcaccgc caccagctcg cgctgcagtt
     5101 cgggcaagta gtcgggggcg gaagcattgc gcccggcgcg ttcatgggcc agaagaatgg
     5161 acaggcgttc cttggcaacg ctggccgtag actttttctc gcccagaaaa aacgacaaaa
     5221 gggaagccat ggcttatttc cctccgaaca ggcgcttgag cagaccctgc ttgggtgcat
     5281 cggtgaagcg catgggaacg tccttgccca agaagcgttc aatgacgtcc ttgtaggctt
     5341 cagagacatc gctgccttgt atgtgcacgg caggcacgcc ctggttggag gcctgtagca
     5401 ccgattcact ttcggggata acaccaatca agtcaatgcg aaggatatcc ttgatgtcat
     5461 cgattgacag catctggccg gtgttgacac ggctggggtt gtagcgggtg atcagtaggt
     5521 gttccttgat agccccgccc ccttccatgg cgcgcttggt cttgctggcc agcatgccca
     5581 gaatgcggtc cgaatcgcgc accgaa
//
LOCUS       DAOSJM010000143         5580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13062_length_5580_cov_4.014842, whole genome shotgun sequence.
ACCESSION   DAOSJM010000143 DAOSJM010000000
VERSION     DAOSJM010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13062_length_5580_cov_4.014842"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1920
                     /gene="ligA"
                     /locus_tag="PKX82_06410"
     CDS             <1..1920
                     /gene="ligA"
                     /locus_tag="PKX82_06410"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOF51920.1"
                     /translation="AVPMLSIRTETDTESTGAEAFDARVRKELELSETDPPVEYVAEL
                     KFDGLAMNLRYEHGVLVQAATRGDGETGEDVTQNIRTIGQIPLRLPADVAPTLPASRG
                     SLPPEGALAPWGGPAALGVPPVLEVRGEVYMRRDDFEALNERQRNRIAAGEKGEKTFV
                     NPRNAAAGAVRQLDPAIAAQRPLSFFAYGLGEVTPIEQGGPAFSTHYAMLQTLKSWGF
                     PVAAQVGIAQGAPELIAYHRRIGAKRDQLPFDIDGVVYKVNSLALQQRLGFVTREPRW
                     AVAHKYPAQEQLTTVLAIDVQVGRTGKLTPVAKLAPVFVGGVTVTNATLHNEDEARRK
                     DVRLGDTVIVRRAGDVIPEVVSVLVEKRVADAPQFTMPHVCPVCQSAAVREEGEADYR
                     CTGGLFCSAQRKQSILHFAQRRAVEVEGLGDKLVEQLVDAGIVNTLPDLYRLGLTALA
                     AMDRMAEKSAQNLLDALQKSKQTTLPRFLFGLGIRHVGEATAKELARHFGMLDAIMDA
                     TEEALLGVSDVGPIVAQSIRTFFAQPHNREVVEALRKPEPDGCGLHWEEGEPAAQAPK
                     LLAGKTFVLTGTFPTLSRDQAKDLLEAAGAKVAGSVSKKTSYVVAGTDAGSKLVKANE
                     LGVPVLDEAAMLAML"
     gene            1945..3066
                     /locus_tag="PKX82_06415"
     CDS             1945..3066
                     /locus_tag="PKX82_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51921.1"
                     /translation="MWFLFGFITLISFSVYFGIKRLDAKWSGQWAGSGSIPYEYEIVR
                     NKKKTLRFMVGVEGPSGYDFAFKRETWVDRLFKFVGLSIEHQVGSVNFDKLVYVVSND
                     GHLLTSLSLDSKVQEAVVQLFKLSRYECHVTQVRCTQGRIWATFKVGRLFSTDSDLLR
                     LHKIFPQAAALLQQASTQLKARAPLEQGTQRDRFILRAAAILALSTGLVVNGIAHGVR
                     VLWESGSFTVDIQRLWVYAALCGGGLVAALVLVAIGFLGRSSRAHLVLIELILIGSAG
                     AVLTCFAELRDLNMEMDNSQASSYQVLIQSKSISRSRKGGTHYYLLVPDWNREKDDRR
                     IKVSREFFDSVRVGQQLEVKQRKGYLGLRWVEPIQGAAW"
     gene            complement(3112..3537)
                     /locus_tag="PKX82_06420"
     CDS             complement(3112..3537)
                     /locus_tag="PKX82_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HOF51922.1"
                     /translation="MSEPSHLSPDAVPKPLPRWMQWLLRALALLSLGLGFLGMFLPVL
                     PTTPFILLAAWAATRSSPRLLAWLENHRLFGTTLRDWRRGGVVSRKAKWAATWTMSAS
                     AVVLLLTVGKLWAAAAAIACMATVLVWLWQRPETFTPPP"
     gene            3574..4113
                     /gene="def"
                     /locus_tag="PKX82_06425"
     CDS             3574..4113
                     /gene="def"
                     /locus_tag="PKX82_06425"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOF51923.1"
                     /translation="MTVREILKMGDPRLLRVAQPVTAFDSDALHLLITDMLDTMRAAS
                     GAGLAAPQIGVDLQLVIFGSNARNPRYPDRPVVPPTVLINPVLTPLGSEQESDWEGCL
                     SVPGLRGVVPRWSRLHYSGYDQYGDPFERTVDGFHARVVQHECDHLIGKLYPMRMQDF
                     TQFGYTDVLFPGIAEAEDD"
     gene            4384..5007
                     /locus_tag="PKX82_06430"
     CDS             4384..5007
                     /locus_tag="PKX82_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51924.1"
                     /translation="MTTALFAVVLASGCAVTPPAAERMVVAPTGTVLTYHRKSSGSLG
                     AFDGKVVWNQSNSSWQGQPMVAVGSAVAGTTLHEPARFGIVAIVNPAGQPVVSYTPPI
                     AYQWPLEVGKAWTSSHTATLHASGKTIPVQVNWKVEAWEDITVPAGTFKAYKLVWADN
                     LGETETRWVNPQASLATIKRHVERPATHPQGAGVLDAELQSYVFPGK"
     gene            complement(5016..>5580)
                     /locus_tag="PKX82_06435"
     CDS             complement(5016..>5580)
                     /locus_tag="PKX82_06435"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901360.1"
                     /note="catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein"
                     /protein_id="HOF51925.1"
                     /translation="RLSPVGEGLQVLVYTRDQSDLFARICGYFDQAGFSILDARIHTS
                     KTGYALDTFQVVSSSDLGDHYRELINMLEAGLSQSVAQAGPLPTPTRGRVSRRVKSFP
                     IAPRVTLRPDEKAQNWLLNISASDRLGLLYCVARVLARHQIEVQLAKVATLGERVEDT
                     FLIHGPQLQHNRAQIEIETELLDALAA"
BASE COUNT         1081 a         1609 c         1771 g         1119 t
ORIGIN      
        1 gcggtaccca tgctgagcat ccgcaccgag accgataccg agtccaccgg tgccgaggcg
       61 tttgacgccc gcgtacgcaa ggaactggaa ctgtccgaga ctgatccacc tgttgaatat
      121 gtggccgagc tgaagtttga cggcttggct atgaacctgc gctatgaaca cggcgtgctg
      181 gtgcaggcgg ccacgcgtgg tgacggcgag accggtgaag atgtcacaca gaacattcgc
      241 accatcggcc agattccctt gcggctacca gcggatgtgg cccccacgct ccccgcttcg
      301 cgtggttcgc tgccccccga gggggctctc gcgccttggg gcggcccggc ggcgctcggt
      361 gttccgcctg tgctggaagt acgtggcgag gtctacatgc gccgcgacga tttcgaggca
      421 ctcaacgaac gccagcgcaa tcgcattgcc gcaggagaga agggcgaaaa gacctttgtg
      481 aacccgcgca atgcggctgc cggtgcggtg cgccagctcg accctgccat tgccgcccaa
      541 cgccccctga gtttctttgc ttacggcctg ggcgaggtta cgccgataga gcagggcggt
      601 ccggcgttca gcacgcacta tgcgatgctg caaaccctca aatcatgggg ttttccggtt
      661 gcagcccagg tgggtattgc gcagggcgct cctgaattga tagcgtacca ccggcgtatc
      721 ggcgccaagc gtgaccagct gcctttcgat attgacggtg tggtctacaa ggtcaacagc
      781 ctggccctgc agcagcgcct gggttttgtg acccgtgagc cgcgctgggc cgtggcgcac
      841 aaatacccgg cgcaggaaca gctcaccacc gtgctggcta ttgacgtgca ggtgggccgc
      901 accggcaagc tcacgcccgt ggccaagctg gccccggtgt ttgtgggtgg cgtgaccgtc
      961 accaatgcca ccctgcacaa cgaagacgaa gcacgccgca aggacgtgcg cctgggcgat
     1021 accgtcatcg tgcgtcgcgc tggcgatgtc attcccgaag tggtgtccgt attggtggaa
     1081 aagcgtgtgg ccgatgcgcc gcagttcacc atgccccatg tgtgcccggt gtgccagtcc
     1141 gcagccgtgc gcgaggaagg cgaagccgat taccgttgca ccggcggctt gttttgtagc
     1201 gcgcagcgca aacagtccat cctgcacttt gcccagcgcc gtgcggtgga agtcgagggg
     1261 ctgggtgata agctggtcga gcaactggtg gacgctggca ttgtcaatac gctgccggac
     1321 ctgtaccgcc tggggctgac tgcgctggcc gctatggatc gaatggccga gaagtccgcg
     1381 cagaacctgc tcgatgcgct acaaaaatcg aagcagacaa ccctgccgcg gtttctcttt
     1441 ggactgggca tccgccatgt gggcgaagcc accgccaagg agctggcccg ccactttggc
     1501 atgcttgatg ccatcatgga cgctaccgaa gaagcgctgc tgggcgtgag tgatgtgggc
     1561 cccatcgtgg cccaaagcat ccgcaccttt tttgcccagc cgcacaaccg cgaggtggtg
     1621 gaagcgttgc gaaagcccga acccgatgga tgtggcctgc actgggaaga gggcgaacct
     1681 gcggcccagg cccccaaact gctggcgggc aagacttttg tgttgaccgg caccttcccc
     1741 acgctgagcc gtgaccaggc caaagacctg ctggaagccg ccggcgccaa ggtggctggc
     1801 tcggtcagca agaaaaccag ttatgtggtg gcaggtaccg atgcgggtag caaactggtc
     1861 aaggcgaatg aactcggtgt gccagtgctg gacgaagcag ccatgttggc gatgctgtag
     1921 acggataggt aaccgtagcc ttgtatgtgg ttcctgtttg gttttattac gctgatcagt
     1981 ttcagtgtgt actttggcat caaacgactt gatgcgaagt ggagcggaca gtgggcgggc
     2041 tcaggttcta tcccgtatga gtacgagatt gttcggaaca agaaaaagac cctccgattc
     2101 atggtgggag tggagggccc ttctggctac gactttgcat tcaaacgaga aacctgggta
     2161 gatcgacttt tcaaatttgt gggcttgtcg atagaacacc aggtcggttc cgtgaacttc
     2221 gacaaattgg tgtacgtggt ttctaacgat ggacaccttt tgacgtcact ttcgctggac
     2281 agcaaggtgc aggaggctgt ggtccagttg ttcaaattaa gtcgctatga gtgccatgtg
     2341 acacaagtcc gttgcacaca ggggcggata tgggcgacgt tcaaggtagg acgtctgttc
     2401 agtaccgact ccgacttgct acgacttcac aagatatttc cgcaagctgc cgctttactg
     2461 caacaggcca gtacgcagtt aaaagcgcgt gctcccttgg aacaaggaac acagcgcgac
     2521 cgcttcatct tgcgggcggc cgccatactg gcactgagta ccgggctggt agtgaatggc
     2581 attgcgcatg gtgtaagggt actgtgggaa tctggcagtt ttaccgtgga tatccagcgt
     2641 ttgtgggtat acgcggcctt atgcgggggg ggcctggttg ctgctttggt gcttgttgct
     2701 attggttttt tgggccgaag ttcccgcgcg catttggtac tgatcgagtt gatactgatt
     2761 ggctcggcgg gtgccgtact cacctgcttc gccgagttgc gtgacctgaa catggagatg
     2821 gataactcgc aggcctccag ctatcaggtt ctgatccaga gcaaatctat cagccgctcc
     2881 agaaaggggg ggacgcatta ctacctgctg gtcccagact ggaatcgcga aaaagacgat
     2941 cgacgcataa aagtatcgcg tgagtttttt gactcagttc gcgtagggca gcaactcgag
     3001 gtcaaacaaa ggaagggtta tctggggctg cgctgggttg agccgattca aggtgcagct
     3061 tggtaaatgc ggggacgtgg ggctctttta tcatcacccg cgccgtgagc ctcagggtgg
     3121 tggtgtgaac gtttcaggcc gttgccacaa ccagaccagc accgttgcca tgcaggcaat
     3181 tgctgccgcc gcggcccaca gcttgcccac ggtgagcagc aggaccactg cactggcaga
     3241 cattgtccag gtcgctgccc atttggcctt gcggctgacc acgccgccgc gacgccagtc
     3301 gcgcagcgtg gtgccaaaca gccggtggtt ctccagccag gccagcaggc gcggtgagct
     3361 gcgcgtcgcg gcccacgccg cgagcagaat gaaaggtgtc gtgggcaaca cgggcaggaa
     3421 catgcccaga aaacccaggc ccaggctcag cagcgccagc gcgcgcagca gccattgcat
     3481 ccaccggggc agcggcttcg gcaccgcgtc cggtgaaaga tgggagggct ctgacatgcc
     3541 ctggattgtg ccgtgtgaca gaaaggactg accatgaccg tgcgtgaaat tctcaagatg
     3601 ggcgacccgc gcctgctgcg cgtggcgcag ccggtaaccg cgtttgacag cgatgcactg
     3661 catctgctca ttaccgacat gctcgacacc atgcgcgctg ccagcggagc tggcctggcc
     3721 gcgccccaaa tcggcgtgga tttgcagctg gtgatttttg gcagcaatgc gcgcaacccg
     3781 cgctaccctg accgtccggt ggtgccgccc accgtgctga tcaatccggt gctcacgccg
     3841 ttgggcagtg aacaggaaag cgactgggag ggttgcctgt cggtccccgg cctgcgcggt
     3901 gtggtgccac gctggtcccg cctgcactac tcgggctacg accagtacgg cgacccgttc
     3961 gagcgcaccg tggacggctt ccacgcccgc gtggtgcaac acgaatgcga ccacctgatc
     4021 ggtaagctct accccatgcg gatgcaggac ttcacgcagt tcggttacac cgatgtgctg
     4081 tttccaggga ttgctgaggc ggaggacgac taaacgattt gctataaaaa gcatagcgat
     4141 atgcgcttat tcctcggggg ataagcccct aatcgcttac ataaatgctg caagcgcgtc
     4201 cagcggctca gtctcgatct cgagctgtgc ccggatttgg gctttgattt gtatcaacga
     4261 ctgcagagac tgccctgtct acgataaata cggtggaggg tgtcatcagt ggatgtcgcc
     4321 tggatccacc gcataaacga gggttctgtc atgtccagct atcgtccctt ccggcttctg
     4381 ccgatgacta cagcactttt cgccgtcgta ctggccagcg gttgcgcggt gactccaccg
     4441 gctgcagagc gcatggtggt tgcccccacg ggaactgtat tgacctacca ccgcaagagt
     4501 tcaggcagtc tgggcgcttt tgatggcaag gtggtctgga accagagcaa cagcagttgg
     4561 caggggcagc ccatggttgc cgtgggttct gccgtggcag gtactacgct gcacgagcct
     4621 gccagattcg ggatcgttgc catcgtaaat cctgccggac agcccgtggt ttcctacacc
     4681 ccgccgattg cctaccaatg gccgctggaa gttggcaaag cctggacttc ctctcacact
     4741 gccacactgc atgccagtgg caagaccatt ccggtgcagg tgaactggaa ggtggaggcc
     4801 tgggaggaca tcactgttcc ggctggcaca ttcaaggcgt acaagctggt gtgggccgac
     4861 aacctcggtg aaacagagac gcgttgggtg aatccacaag ccagcctggc caccatcaag
     4921 cggcatgtgg agcgtcctgc cacccaccca cagggagcgg gcgtgctcga tgcagagttg
     4981 cagtcgtacg tgttccccgg caagtaacgg gcgggtcaag ccgccagcgc gtccagcagt
     5041 tcggtctcga tctcgatctg cgcccggttg tgctgcagct gcgggccgtg gatgaggaag
     5101 gtgtcttcca cgcgttcgcc cagcgtggcg accttggcca gttgcacttc gatctggtgg
     5161 cgggccagaa cccgggccac gcaatagagc aggcccagcc ggtcactggc ggaaatattg
     5221 agcagccagt tctgggcctt ctcatcgggc cgcagggtga cgcgcggcgc aatcggaaag
     5281 ctcttgaccc ggcgtgacac acggccgcgg gtgggtgttg gcaacgggcc tgcctgggcc
     5341 acggactggc tcagtccggc ttcgagcatg ttgatgagtt cgcggtaatg gtctcccagg
     5401 tccgaactgg acacaacctg gaaggtatcc agcgcgtaac cggttttgct ggtgtggatg
     5461 cgcgcatcca gaatactgaa tccggcctgg tcgaagtagc cgcagatgcg tgcaaacaga
     5521 tcgctctggt cccgcgtgta gaccagcacc tgcaggcctt cgcccactgg ggacagacgc
//
LOCUS       DAOSJM010000144         5574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13077_length_5574_cov_4.112339, whole genome shotgun sequence.
ACCESSION   DAOSJM010000144 DAOSJM010000000
VERSION     DAOSJM010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5574
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13077_length_5574_cov_4.112339"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PKX82_06440"
     CDS             <1..187
                     /locus_tag="PKX82_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HOF51926.1"
                     /translation="LPTTGALSFMATQDQLSFFYWMRVWGGVGFLVGLLAYLSSFFIG
                     AGANENEPATGMVPGRA"
     gene            199..1002
                     /locus_tag="PKX82_06445"
     CDS             199..1002
                     /locus_tag="PKX82_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HOF51927.1"
                     /translation="MNVLPQHTPPYYAAQGAECQLFEHAWQQQLPLLIKGPTGCGKTR
                     FVEHMAARLGRPLVTVSCHDDLSASDLVGRFLIGQGNTVWSDGPLARAVRNGAICYLD
                     EVVEARKDTTVVLHPLADDRRILPIERTGEELAAPPEFMLVISYNPGYQNLLKGLKPS
                     TRQRFVALSLDYPAPDIERTIVQNETGVSEIIAGQLVRLATALRCLTDHDLEETASTR
                     LLVAAGRLAASGLPLALACRAAIIDALTDDADTAVALREVVRAVIGDES"
     gene            992..2008
                     /locus_tag="PKX82_06450"
     CDS             992..2008
                     /locus_tag="PKX82_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOF51928.1"
                     /translation="MKADVGAQASSTARLQAWRRRTQGGFFLLFLLAPALNLLRFDLH
                     EAQLWFLGMRWSLGIDALARGEVSATQAGITLLLRAFVPAVLFIVVFLGIAYRYGRIY
                     CGWLCPHFSAVETLNTLLHRACGKLSLWDKATTPRAGVDPSPRWWPLFALSCLTMGFV
                     WAITLLTYLLPPAEIWGRLLQGTLTPGPTRFLLVATLLFTLEFAFARHLFCRFGCAVG
                     LFQSLAWMANPRGMVVAFTRERARDCKTCRHETAEGKALPGAAALTALPGNACDAVCP
                     MRLHPRNIKRLMFSCVQCGQCLTACDATQSAQHRSPTLQWEIGEAALRETLRQRREEL
                     QDKR"
     gene            2008..3837
                     /locus_tag="PKX82_06455"
     CDS             2008..3837
                     /locus_tag="PKX82_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOF51929.1"
                     /translation="MEELVGRWWHEALTHWTRPEHAAFSVSLDEAKKSIGIMFRAAGG
                     AGHVRLTEATSQRTGGAQPWLNRLAGTPQRAALTVLEPQVLALPPALAVFPDRALNHD
                     LYLWLALLSAHYTHGNGWVADNLQATDHALQAFPGFAPRYQALRDAHLAQRPDPAALK
                     GRHAAAERCIQTALRDQTPDYLDLVCADVAPVWLWMGAVESFAPGAERSTQPTTPTDP
                     RKAADKNDTQRRRASTRQYDTERNPFILPFRAEALMSWNEMVRVNRSTDDEDDGSAMR
                     AANDMETLSITPDGQSLAARVKFDLDLPSSSADDLPLGPGTHFPEYDYRKGVLIPAHC
                     CLQERIARPGTAFVPSPALRNTARAMRKRLEILRDAPRQQRGQQSGDDIDLDAWIRLQ
                     ADAVGDQALRSDTPAVYTRRVRGERSLSTLLLADLSQSTDAYASDTERVIDVIRDALY
                     VFGEALTAVGDPFAMWGFSSVRRQNVRMQRILDFDERWSDASRARVGAIRPGYYTRMG
                     AAVRHATAQLQVRPERKRLLMLLTDGKPNDLDVYEGRYGLEDTRHAIHEARTAGLTPF
                     CVTIDTDAHDYLPMLFGRQGYAMVHRPQDLVRRLTQAWTMLAR"
     gene            3894..4994
                     /locus_tag="PKX82_06460"
     CDS             3894..4994
                     /locus_tag="PKX82_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HOF51930.1"
                     /translation="MRRRAVLGWGAASGLALVPAAHALPPRKLAFPRDAGAHPDFRTE
                     WWYITGYANAGGEQAALGFQVTFFRSRVEATQTMQSSMAAKQLLFAHAAVTDVRGKKL
                     WHDQRIARWSGAAPGTNPVDTAHASTRDTAITLNHWTLLRDGSDLQAKVTGTDFALDL
                     RFVQTQPVLLQGADGLSRKGPDAGQASYYYSLPQLEVSGSITLQGKVRTLGAGSTAWL
                     DHEWSQEVLHPQAVGWDWMGINLFDGSALTAFQLRDKEGNALWDGGSFRSAPSAGGTR
                     HAFARGEVLFRPLRSWRSPLSQASYPVEWIVRTPADYYTVKAVVDNQELDSRNSTGAI
                     YWEGLCEVWNSNNRLVGRGYLEMTGYAAALKL"
     gene            5120..>5574
                     /locus_tag="PKX82_06465"
     CDS             5120..>5574
                     /locus_tag="PKX82_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOF51931.1"
                     /translation="MTSSDRPKSTNPKSLSGLLPFLRPYRGRIGLALLFLVLAAGATL
                     AFPVALRSLIDGGLVQADKGAQVMALREHFAALFAVAVALGLFSACRFYMVSWLGERV
                     TADLRNAVYSHVLKQSPEFFETTQTGEVLSRLSADTTLVQTVVGSSLSMG"
BASE COUNT          971 a         1877 c         1735 g          991 t
ORIGIN      
        1 cctgcctact acaggcgccc tctccttcat ggcaacgcag gaccagctga gcttcttcta
       61 ctggatgcgc gtctggggcg gtgtgggctt cctggtgggc ctgctcgctt acctcagcag
      121 cttcttcatt ggcgcgggtg ccaacgagaa cgaacctgcc acgggcatgg tgcccggacg
      181 tgcctgaaat tgaccgctat gaacgttctt ccacagcaca cgccgcctta ctacgcggcg
      241 cagggagctg aatgccagtt gtttgaacat gcatggcaac aacagttgcc gctgctgatc
      301 aagggaccca ccggctgtgg caagacccgt tttgtggaac acatggcggc ccgtctgggc
      361 cgtccactgg tcaccgtgtc ctgccacgac gacctcagcg cctccgatct cgtggggcgc
      421 tttctgattg gccagggcaa tacagtgtgg tctgatggtc cgttggcccg cgcagtgcgc
      481 aacggcgcca tctgctacct cgatgaggtg gtcgaagctc gcaaggacac caccgtggtg
      541 ctgcatccac tggcggacga ccggcgcatt ttgccgatcg aacgcacggg ggaagagttg
      601 gcagcgccac cagaattcat gctggtgatc tcgtacaacc cgggttacca gaatcttctc
      661 aagggcctca agcccagcac acggcagcgc tttgtggcgt tgagcctgga ctaccctgcc
      721 cccgacattg agcgcaccat cgtgcagaac gagacaggtg tctccgaaat catagctggg
      781 caactggtgc gcctggcaac cgcgttacgc tgtctgaccg atcatgacct ggaagaaaca
      841 gccagcaccc ggctgctggt agccgctggg cgcctggccg catcgggtct gccactggca
      901 ctggcatgcc gtgccgccat cattgatgcg ctgacggacg atgcggatac cgccgtcgcg
      961 ctgcgcgagg tggtacgtgc tgtcatcggc gatgaaagct gatgtaggcg cacaggcctc
     1021 cagcaccgcg cgtctgcagg catggcgccg ccgaacacag ggcggctttt ttcttctgtt
     1081 tttgctggct cccgcactga acctgctgcg ttttgacctg catgaggccc aactgtggtt
     1141 tctgggcatg cgctggtcgt tgggtattga tgcgctggca cgcggcgaag tcagcgcaac
     1201 ccaggcgggc ataaccctgc tcctgcgcgc ttttgtgccc gccgtgctct tcatcgtcgt
     1261 atttctgggc attgcctacc gctacgggcg catctactgc ggctggttgt gcccccattt
     1321 ttctgccgtt gaaaccctca acacgctgct gcaccgcgcc tgcggcaagc tcagcctgtg
     1381 ggacaaagcc accacccccc gcgccggcgt cgacccgtct ccccggtggt ggccactgtt
     1441 tgccttgagc tgcctgacga tgggctttgt gtgggccatc acgctactga cctacctgtt
     1501 gcctcccgca gaaatctggg gccggctgtt gcaaggcacc ctgacgcccg ggcccacacg
     1561 ctttctgctg gtggccacgt tgctgttcac gctggagttc gccttcgcac ggcatctgtt
     1621 ttgccgcttt ggttgcgccg tagggctgtt ccagagtctg gcctggatgg ccaacccacg
     1681 gggcatggtg gtggctttca cccgcgaacg cgcacgcgac tgcaagacct gccgccatga
     1741 aaccgccgaa ggcaaggcgc tgcccggggc agccgccctg acggcgcttc cgggaaacgc
     1801 ctgcgatgcg gtttgcccca tgcgcctgca cccgcgcaat atcaagcggc tcatgttctc
     1861 ctgcgtgcaa tgcggccagt gcctgacagc gtgcgatgcc acccagtccg cgcagcaccg
     1921 tagcccaacc ctgcaatggg aaatcggcga agcggctttg cgtgaaaccc tgcgccagcg
     1981 ccgcgaagaa ctccaggaca agcgctgatg gaagaactgg taggccgctg gtggcacgag
     2041 gcgctgaccc actggacgcg tcccgagcat gcggcttttt cggtcagcct ggatgaagcc
     2101 aaaaaatcca tcggcatcat gtttcgcgcg gcgggcggcg caggccatgt gcggctcact
     2161 gaagccacgt cgcagcgcac gggcggtgcc caaccctggc tgaaccgcct ggcgggcacg
     2221 ccccaacgcg cggcgctgac cgtgctggag ccgcaggtac tggccttgcc ccccgcgctg
     2281 gccgtttttc cggaccgcgc gctgaaccac gacctgtatc tatggctggc attgctgtcc
     2341 gcacactaca cgcacggcaa tgggtgggtg gcggacaacc tgcaagcgac tgaccatgca
     2401 ctgcaggcct ttcccgggtt tgcaccgcgt taccaggcgc tgcgcgacgc ccacctggcc
     2461 cagcgcccgg acccagccgc actcaaggga cgccacgctg cagccgaacg ctgcatacag
     2521 accgctctgc gcgaccagac gccagattat ctggacctgg tctgtgcgga cgtagctccg
     2581 gtatggctct ggatgggtgc cgtcgaatcg tttgcgccag gcgctgagcg gagcacccaa
     2641 cccaccacgc caactgaccc acgcaaagca gcggacaaga acgacaccca gcgccgccgc
     2701 gccagcacac gccagtacga caccgaacgc aacccattta tcctgccgtt ccgcgccgag
     2761 gcgctgatga gctggaacga aatggtgcgt gtcaaccgca gcaccgacga cgaagacgat
     2821 ggcagcgcca tgcgcgccgc caatgacatg gagacactga gcatcacccc cgacggccag
     2881 tctctggccg cacgggtgaa gttcgacctg gacctgccca gcagcagcgc agacgatctg
     2941 ccgctgggac ctgggaccca ctttcccgag tacgactacc gcaagggcgt gctgatcccg
     3001 gcacactgct gcctgcagga gcgcatcgcc cgcccgggca ccgcctttgt gccgtctcca
     3061 gccctgcgca acaccgcgcg tgccatgcgc aagcgcctgg aaattttgcg cgatgcgccg
     3121 cgccagcagc gcggccagca aagtggtgac gacattgatc tggacgcctg gatccgactg
     3181 caagccgacg ctgtgggcga ccaggccttg cgcagcgaca cccccgccgt ctacacgcgc
     3241 cgggtacgtg gggaacgcag cctctccacc ctgctgctgg cggacctgtc gcaatccacc
     3301 gatgcctatg ccagcgacac cgagagggtg atcgatgtga tccgcgatgc actctatgtg
     3361 tttggcgaag cactcaccgc cgtgggcgac ccgtttgcca tgtggggctt cagctcggtg
     3421 cgacgccaga atgtgcgcat gcagcgcatc ttggattttg acgaacgctg gagcgacgcg
     3481 tcccgtgcac gcgtgggcgc catccgcccc ggctactaca cccgcatggg cgcagcggtg
     3541 cgccatgcca cagcgcaact gcaggttcgg cccgagcgca agcgcctgct gatgctgctg
     3601 accgacggca aacccaatga cctcgatgtg tacgagggtc gctatggcct ggaggacacg
     3661 cgccacgcca tccacgaggc acgtaccgcc ggactgacgc cattctgtgt gaccatcgac
     3721 accgacgcac acgactacct gcccatgctg tttggccgcc agggctacgc catggtgcac
     3781 cgcccacagg acctggtgcg acgcctgacg caggcttgga ctatgcttgc acgctaatcc
     3841 acacacgggg ggccacacgg gctccagcca cggtgtctgt ttcaagcttc tctttgcgac
     3901 gccgcgcggt cctgggctgg ggcgctgcca gcggcctggc gctggtgcct gccgcccacg
     3961 ccctgccccc gcgcaagctc gcgtttcccc gcgatgcggg cgcacaccct gactttcgca
     4021 ccgagtggtg gtacatcacc ggctatgcca atgcgggtgg cgagcaggca gcactcggct
     4081 ttcaggtgac gttctttcgc agccgggtgg aggcgacaca gacgatgcag tccagtatgg
     4141 ccgccaaaca gctactgttt gcacacgccg ccgtgaccga tgtgcgcggc aaaaaactct
     4201 ggcacgacca gcgcattgcc cgctggtccg gcgcggcgcc gggcaccaac ccggtggaca
     4261 cggcacatgc cagcacccgg gacacggcga tcaccctgaa ccactggacc ctgctgcgcg
     4321 atggcagcga cttgcaggcc aaagtcactg gtacggactt cgcgctggac ctgcgctttg
     4381 tgcagacgca gcccgtgctg ctgcaaggcg cagatggcct gtcgcgcaag gggccggacg
     4441 ccgggcaggc cagctattac tacagcctgc cgcaactgga agtcagcggc agcattacgc
     4501 tgcaggggaa ggttcgcacg ctgggcgcag gcagcacggc atggctggac cacgagtgga
     4561 gccaggaggt gttgcacccg caggccgtgg gctgggactg gatgggcatc aacctgttcg
     4621 acggcagcgc gctgaccgcc ttccagctac gcgacaagga gggcaacgcc ctgtgggatg
     4681 gcggatcgtt ccgcagcgca ccctctgccg ggggcacccg ccatgcgttc gcgcgcggcg
     4741 aggtgctgtt ccgccccctg cgcagctggc gcagcccgct gagccaggcc agctatccgg
     4801 tggagtggat cgtgcgcaca ccggccgact actacaccgt caaggcggtc gtggacaacc
     4861 aggagctcga cagccgcaac tccacaggcg ccatctattg ggagggtctg tgcgaggtgt
     4921 ggaatagcaa caaccgcttg gtcgggcgcg ggtatctgga aatgaccggc tacgccgcgg
     4981 cgttgaaact gtgatggtcc actacaaacg ctacactttt agtagcaaca tgcgcaatat
     5041 ggacgggggc tagaggccaa tttctctata tttatgccct tgccttgcct ccaccgcctt
     5101 gacccccatc taagcagcca tgacttcttc tgaccgcccc aaatccacca accccaaatc
     5161 gctctccggt ctgctgccgt ttctgcggcc gtatcgaggc cgcattggcc tggcgctgct
     5221 gtttctggtg ctggcggccg gggccacgct ggccttcccc gtggcactgc gcagcctgat
     5281 cgacggtggg ctggtgcagg ccgacaaggg tgcgcaggtg atggcgctgc gcgaacactt
     5341 tgcggcgctg tttgccgtgg ccgtggcact gggcctgttt tcggcctgcc gcttctacat
     5401 ggtgagctgg ctgggcgaac gcgtaaccgc cgacctgcgc aatgcggtct acagccacgt
     5461 gctcaagcag agccccgaat tctttgaaac cacgcaaacc ggcgaagtgc tctcgcgcct
     5521 gtcggccgac accacgctgg tgcaaaccgt ggtgggctcg tccctgagca tggg
//
LOCUS       DAOSJM010000145         5556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13127_length_5556_cov_4.115070, whole genome shotgun sequence.
ACCESSION   DAOSJM010000145 DAOSJM010000000
VERSION     DAOSJM010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13127_length_5556_cov_4.115070"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PKX82_06470"
     CDS             <1..430
                     /locus_tag="PKX82_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF51932.1"
                     /translation="DWAPSTRNLAPLMLHLASPVLTPLFGYFPGKRLGMVGDMPPGVM
                     RQWTRWCRHPGFAWGAEPHKLLPVLAAARYPIRALAFSDDEIMTLRAVHKLLQATPNA
                     PSTVQTITPQSVGAKRIGHLGAFRAQHEKQLWPRVADQLV"
     gene            complement(499..3996)
                     /locus_tag="PKX82_06475"
     CDS             complement(499..3996)
                     /locus_tag="PKX82_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOF51933.1"
                     /translation="MASNFSFLQVEWPALYAEGLRAEQAALTDPRTACFYARRTLELA
                     VVWLFQAEGGRSGRLQMPYKPDLSAFLFEPSFKVLVGPALHAKMDVVRKQGNNAVHSA
                     RPITAADATAVLRELFQVAFWLARNYGRNVAARPDPALQFRADLLPRPLDAAAEQAAA
                     QASAQATQAALQKLATLADELAARDAALTTAQQKTAALDAELAQLRAEVAAAKAANTA
                     TPDTHDYNEAETRDLYIDLLLKEAGWKLDQPRDREFEVQGMPNAQGKLEGTGYVDYVL
                     WGDDGKPLAVVEAKRTRRDARVGQQQAKLYADCLQTQFGQRPLIYTTNGYEHWFWDDT
                     TYPPRPVQGFHKKDELQLLIQRRTSAKPLAGVSINPDIVERHYQLRAIRRIGETFERD
                     RQRKALVVMATGAGKTRTVIALVDLLQRANWAKRILFLADRVALVNQAANAFKAHLPD
                     AAAVNLVTDKATEGRVYVSTYPTMMGLINETDEGLRRFGVGHFDLIIVDEAHRSIYQK
                     YKAIFAYFDALLVGLTATPKDEIDRNTYSLFELENGVPTDAYGLEDAIAEGYLVPPRA
                     VSVPLKFQREGIKYAELSVDERAQWDELDWSDDDGEGHAPDEVGAEAVNKWLFNTDTV
                     DKVLELLMTQGHKVAGGDRLGKTIVFAKNNAHANFIADRFNANYPHYAGQFARVITYQ
                     TEYAQSLIDDFSVKEKAPHIAISVDMLDTGIDVPEVVNLVFFKIVRSKAKFWQMVGRG
                     TRLCKDLFGPEQDKQEFLIFDFCQNLEFFSQNLEGSKGNVAEPLSQRTFKARLELLSV
                     LDEQLAQEQGAEGVKVAEPAAGYGLTAAAMRADTASHLHAVVAGMRLDNFVVRPQRRW
                     VEPWSIATAWHTVTGDQLVELAQHVSGLPSAVRDDDEEAKRFDLLMLRTQLGSARGDM
                     GFARLREQVRALADGLSELGSIPDVKKHMVLIEAVAGEEWWQDVTLPMLEQARRHLRG
                     LIKLLEKTRRKVVYTDFEDAVGETSEVALPLGGSAGNFERFRLKVRAYLRAHENHITL
                     HKLRRNQPLTATDLAELERLLIDSGTGTAQDVARAGQEAHGLGLFVRSLVGLDREAAT
                     QALNGFVAGKTLSANQLEFVNLIVTHLTERGVMDVGLLYEPPFTSYAPQGPDALFTSA
                     QVDELFGVLDHIKATARAA"
     gene            complement(4066..4431)
                     /locus_tag="PKX82_06480"
     CDS             complement(4066..4431)
                     /locus_tag="PKX82_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOF51934.1"
                     /translation="MSKEDILRAQDYLAHIVEAIDRIHRYVDDMVELSFLSDEKTQDA
                     VVRNFEILGEASNNIEKHHPAFAQAHVDVPWALMYTMRNRVAHGYFKVDYELVWKTIY
                     ADLPELRAQVAALIDSQKN"
     gene            complement(4428..4718)
                     /locus_tag="PKX82_06485"
     CDS             complement(4428..4718)
                     /locus_tag="PKX82_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF51935.1"
                     /translation="MRPSIALQTHRDAIRTIALSHRVTNVRVFGSVVHGDDIEGSDLD
                     ILVEPTAETTMMDIAKIQLELARLLPVAVDVLTPKGLPAKFRDQVIAEAQVI"
     gene            complement(4757..4951)
                     /locus_tag="PKX82_06490"
     CDS             complement(4757..4951)
                     /locus_tag="PKX82_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOF51936.1"
                     /translation="MDRITLEPGKRGGRPCVRDLRITVYDVLSMLSSGMSTAEILDDF
                     PELTEQDILAVLAFAAEPRH"
     gene            complement(5073..5300)
                     /locus_tag="PKX82_06495"
     CDS             complement(5073..5300)
                     /locus_tag="PKX82_06495"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOF51937.1"
                     /translation="MTLPIVWSAQARRQLLASLAHIADEDPLAAELVFKRIEKSLRTL
                     GEFPDIGSPAPVAGVLLTQFPKRGTALTTGL"
     gene            complement(5297..>5556)
                     /locus_tag="PKX82_06500"
     CDS             complement(5297..>5556)
                     /locus_tag="PKX82_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51938.1"
                     /translation="MTTDIQLPKVTQNRIARLAQASGRTPAATLRFVLRDGFDAVERS
                     IQENANADAEFAAGATATHTDVMADAQKLVRQAKRSARAAA"
BASE COUNT         1009 a         1791 c         1652 g         1104 t
ORIGIN      
        1 cgactgggca cccagcaccc gcaatctggc cccactgatg ctgcacctgg ccagcccggt
       61 gctgacaccg ctgtttggct actttccggg caagcggctg ggcatggtgg gcgacatgcc
      121 gccgggcgtg atgcggcagt ggacccgctg gtgccgccac cctggctttg cctggggtgc
      181 agaaccgcac aagctgctac cagtgctggc cgctgcgcgc tacccgatcc gcgcgctggc
      241 cttcagcgac gacgaaatca tgaccctgcg cgcggtgcac aaactactgc aggccacgcc
      301 caacgccccc agcacggtgc aaaccatcac accgcaatcc gtgggcgcca aacgcatcgg
      361 ccacctgggt gcttttcgcg cccagcacga gaagcagctc tggccgcgcg tggcggacca
      421 gctggtctga aaaccaattt gctattactt tagtagctac ctgcgtatat tccacgaggg
      481 ctagaggccg atttggcact aagccgcccg cgcagtggcc ttgatgtggt ccagcacgcc
      541 aaacagctcg tccacctgtg ctgacgtaaa cagtgcgtct ggcccctggg gtgcatagct
      601 ggtaaagggt ggctcgtaca gcaagccaac atccatcaca ccacgctcgg tcagatgcgt
      661 gacgatcagg ttcacaaact ccagctggtt ggcgctcagt gtcttgccgg ccacaaagcc
      721 gttcagcgct tgggtagcgg cctcgcggtc caggcctacc aaagagcgca caaacaggcc
      781 caggccgtgg gcctcctgcc cggcgcgcgc cacgtcctgt gcagtacctg tgccgctgtc
      841 aatcagcaag cgttccagct cggccagatc ggtcgccgtg agcggctggt tgcggcgcag
      901 cttgtgcagg gtgatgtggt tctcgtgtgc gcgcagatag gcccgcacct tgagccgaaa
      961 gcgctcaaag ttgcccgcac tgccgcccag cggcaaggcc acctcagacg tttcacccac
     1021 ggcatcctca aagtccgtgt agaccacctt gcgacgcgtc ttctccagca gcttgatgag
     1081 tccgcgcaag tgtcttcgcg cctgctccag catgggcagg gtcacgtcct gccaccactc
     1141 ctcgcccgcc accgcctcga tcagcaccat gtgcttcttc acatcgggaa tgctgccgag
     1201 ttcactcaag ccatcggcca atgcacgcac ctgctcgcgc aagcgggcaa agcccatgtc
     1261 gccgcgagca ctgcccagct gggtgcgcag catcagcaag tcgaagcgct tggcttcttc
     1321 atcgtcatcc cgcacggcgc tgggcaggcc cgacacatgc tgggccaact ccaccagttg
     1381 gtcgcctgtg accgtgtgcc aggccgtagc aatgctccag ggctccaccc agcggcgctg
     1441 cgggcgcacc acaaagttgt ccagccgcat gccggctacc actgcgtgca aatggctggc
     1501 ggtgtcggca cgcatagccg ccgccgtcag gccatagccc gcagcaggct cggccacctt
     1561 gacaccctca gcgccctgct cctgcgccag ctgctcgtcc aacaccgaca gcaattccag
     1621 tcgcgccttg aaggtgcgct ggctcaaggg ttcggccaca ttgcccttgc tgccttcgag
     1681 gttctggctg aagaactcca ggttctggca gaaatcgaaa atcaagaact cctgcttgtc
     1741 ctgctcggga ccaaaaaggt ctttgcacaa gcgcgtgccg cgccccacca tctgccagaa
     1801 cttggccttg gagcgcacga tcttgaagaa caccaggttc accacctcgg gcacgtcgat
     1861 gccggtgtcc agcatgtcta ctgagatggc aatgtgcggc gccttttcct tgacggaaaa
     1921 gtcgtcaatc aggctctgcg catattcggt ctggtaggtg atgacgcggg caaactgccc
     1981 cgcgtagtgc gggtagttgg cgttgaagcg gtctgcaatg aagttggcgt gcgcattgtt
     2041 cttggcaaac acaatcgtct tacccagccg gtccccgccc gccactttat ggccctgagt
     2101 catcagcagc tccagcacct tgtccaccgt gtcggtgttg aacagccact tgttcaccgc
     2161 ctcggcaccc acctcgtcgg gggcgtggcc ttccccatcg tcatccgacc agtccagctc
     2221 gtcccactgg gcgcgctcat caacgctgag ctccgcatat ttgatgccct cgcgctggaa
     2281 cttcagcggc accgacaccg cgcgcggcgg caccaggtag ccctcggcaa tggcgtcttc
     2341 caggccatag gcatcggtgg gcacaccgtt ttccagctca aacaggctgt aggtgttgcg
     2401 gtcgatctcg tctttgggtg tggccgtcag tcccaccagc agcgcatcaa agtaggcaaa
     2461 gatggccttg tacttttggt agatggagcg gtgtgcctcg tccacgatga tcaaatcaaa
     2521 gtgccccacg ccaaagcggc gcaggccctc gtcggtttcg ttgatcaagc ccatcatggt
     2581 cgggtaggta ctcacataca cacgcccttc agtggcttta tccgttacca gattcaccgc
     2641 tgccgcatcg ggcaagtgcg ccttgaaggc gttggccgcc tggttcacca gcgccacgcg
     2701 gtcggccaga aagagaatgc gcttggccca gttggcgcgc tgcagcaggt ctaccaaagc
     2761 aatcacggtg cgcgttttgc ccgcgcccgt ggccatcacc accagggctt tgcgctggcg
     2821 gtcgcgctca aaggtttcgc caatgcggcg aatggcgcgc agctggtagt gccgctccac
     2881 gatatcgggg ttgatgctca cgcccgccag cggcttggca ctggtgcggc gctgtatcag
     2941 tagttgcagc tcgtcctttt tgtggaagcc ctgcaccggg cgcggcgggt aggtggtgtc
     3001 gtcccagaac cagtgctcat agccgttggt ggtgtagatc agcgggcgct ggccgaattg
     3061 cgtttgcagg cagtcggcat acagcttggc ctgctgctgg cccacgcgcg cgtcgcgccg
     3121 ggtgcgcttg gcttccacca cggccagcgg cttgccatcg tccccccaca gcacgtagtc
     3181 cacatagccc gtgccctcca acttaccttg tgcattgggc atgccctgca cttcaaactc
     3241 tctatcgcgc ggctggtcca gcttccagcc cgcctctttg agcagcaggt cgatgtagag
     3301 gtcgcgcgtc tcggcttcgt tgtagtcgtg cgtgtcgggc gtggccgtgt tggcggcttt
     3361 ggctgcggcc acttcggcgc gcagctgggc cagctcggca tccagtgcgg cggttttttg
     3421 ctgcgcggtg gtcagggctg catcgcgcgc agccagttca tcggccagcg tggccagctt
     3481 ttgcaacgcg gcctgcgtgg cttgcgcact ggcctgggct gcagcttgct ctgctgcggc
     3541 gtccaagggg cgcggcaaca agtcggcgcg gaactgcagc gccgggtcgg gccgcgcggc
     3601 cacgttgcgc ccgtagttgc gcgccagcca aaacgcgacc tgaaacagct cgcgcaacac
     3661 ggcggtggca tcagcggccg tgataggccg cgcactgtgc acggcgttgt tgccctgctt
     3721 gcgcaccacg tccatcttgg cgtgcaacgc ggggccgacc agcactttga aactgggctc
     3781 aaacaaaaaa gccgacaggt ccggcttgta gggcatctgc aggcgaccgc tgcgaccgcc
     3841 ttctgcctga aacaaccaca ccaccgccag ctccagcgtg cgccgggcat agaagcaggc
     3901 ggtgcgaggg tcagtcagcg cggcctgctc agcgcgcagg ccctccgcat acagcgcggg
     3961 ccattcgacc tgcagaaatg agaaattaga ggccattatt tttgacccca tgagggcaaa
     4021 aacgggctga ttgcttgaaa aagcggcaga tttgacctca tagggtcaat tcttttgaga
     4081 atcaatgagg gcggctacct gagcccgcag ctcaggcaag tcggcataaa tggtcttcca
     4141 caccaactcg tagtccacct tgaaataccc atgcgctacc cgattgcgca tggtgtacat
     4201 cagcgcccaa ggcacatcaa catgagcctg cgcgaaggca gggtgatgct tttcaatgtt
     4261 gttagaggcc tcgcccagaa tctcgaaatt gcgcactacg gcatcctgcg tcttttcgtc
     4321 cgagagaaag ctcaactcca ccatgtcatc cacataccgg tgaatccggt caatggcctc
     4381 cacaatgtgg gccaaatagt cctgggcccg cagtatgtcc tctttgctca tatcacctgc
     4441 gcttcggcaa tcacctggtc gcgaaatttg gccggcaagc ccttgggtgt gagcacatcg
     4501 actgccacag gcagcaagcg ggccagctcc agctgaatct tggcaatatc catcatggtg
     4561 gtctctgctg tgggctctac caggatgtcc aagtcgctgc cctcgatgtc gtcgccatgc
     4621 actaccgagc cgaacacgcg cacattcgtt acccggtggc tgagcgcaat cgtgcggatg
     4681 gcgtcgcggt gggtttgaag ggctatggat gggcgcatgg tttgctcctt gattcagtca
     4741 gtgagaaatc gctctgtcaa tgcctaggct ccgccgcaaa cgccaacacg gccaggatgt
     4801 cctgctcagt gagttccggg aaatcgtcca ggatttcagc cgtggacatt ccgctggaga
     4861 gcatggacag cacgtcgtac accgtgatgc gcaagtcgcg cacgcagggc cgcccgccgc
     4921 gcttgccggg ttccagggtg attctgtcca tggtgctctc ttgaaaatgg ggtgtgaatg
     4981 tagattgtgc cgcgtgttgc gcttgctcta aagctcgccg gcaaacggct gctgcctttg
     5041 ccgataccag cgctgaatgc ggatttcgcc cttcacaacc ctgtagtcaa agctgtgccc
     5101 cgttttggga actgggtaag tagcacaccc gccacagggg ccggactgcc tatgtcagga
     5161 aactcaccca aagtacgcag tgacttttca atccgcttga aaaccagttc tgctgccagc
     5221 gggtcctcgt cggcaatgtg cgctaggctg gccagcaact ggcggcgcgc ctgcgccgac
     5281 cagacgatgg gcagcgtcat gcagctgcgc gcgcgctgcg tttggcttgg cgaaccagct
     5341 tttgggcatc ggccatcaca tccgtgtgtg tggcggtggc acccgccgca aactcggcat
     5401 ccgcgttggc gttttcctgg atgctgcgct ctaccgcatc aaacccgtca cgcaacacaa
     5461 agcgcaaggt cgccgcaggg gtgcgcccag acgcttgtgc caagcgggca atgcggttct
     5521 gggtgacttt aggtagttga atgtcggtgg tcatgc
//
LOCUS       DAOSJM010000146         5439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13475_length_5439_cov_4.147660, whole genome shotgun sequence.
ACCESSION   DAOSJM010000146 DAOSJM010000000
VERSION     DAOSJM010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13475_length_5439_cov_4.147660"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="PKX82_06505"
     CDS             <1..1039
                     /locus_tag="PKX82_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOF51939.1"
                     /translation="AALGLPSRDALEALMHATWTQALQTPVCDVLWRTSDDAGETLLP
                     SSLVQLLQAAAMDTTQGGIDPRLERAVPTAPVHPPQPVGAALPVKHLSASAYEDLRQC
                     PYRFFAMRQLGLKTVDELDGEVDKRDFGLWLHAVLAQFHAALQSGPTEDATVRSRMLD
                     QTAEEITRSMALPEGEFLPFMAAWPQVRDGYLKWLTAHEAQGARFASGETSHRQRVGD
                     VQLVGRIDRTDAFADGTVMVLDYKTEPVAKSRSRVKEPLEDTQIAFYAALLDADTLRG
                     AYVNVGERDGTAMSEQEHLVEARDALLEGIQMDMQRIRDGAALPALGDAGACDFCQAR
                     SMCRKDFWSVP"
     gene            1036..4383
                     /locus_tag="PKX82_06510"
     CDS             1036..4383
                     /locus_tag="PKX82_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOF51940.1"
                     /translation="MSAVHPSPAAAHAAFEHNGVVVAREQFYAIACDPRRSVAVEACA
                     GAGKTWMLVSRMVRALLDGCAPQDILAITFTKKAAGEMRQRLQQWLAEFAQADDATLR
                     DALKVRGLVQDATDAQLQDLRALHAKLLRNGRPVQIRTFHSWFAALLRSAPLAVLDAM
                     GLPTQYELLENDAQAVAQVWRRFQTRAVQDSDARADYMAAVAAYGRHQTHKALESALS
                     KRVEFALADAHGVVASSIAHFAEQFPEFDGLTDPLQRVDAPAVRQHLVETARALGAST
                     LVTCTKAAVALERAMTDGDVPSMFDALLTQAGKPRKFSDKLAGIAMVQAAQELLLQIQ
                     EARHQHDAWVHQQRMTRLARGLLEDYAALKRERGWIDMGDLERAALVLMSDPQLSGWV
                     QERLDARVRHLLIDEFQDTNPLQWQALSAWLSGYTGAGTAPSVFIVGDPKQSIYRFRR
                     AEPQVFRAAKTFVVQGLGGDILSCDHTRRNAPCVIGVVNSVMQAAQQANEFEGYRAHS
                     TESTQEGQVLALPRILREPKAVAASAGEELDTAAWRDSLTTPRAQAEDTRKTLECRQA
                     ARWLAQWLRDSEGRKPSEVMVLARKRERLGLMQAELAALHIPAQQPEKTDLCDMPEVQ
                     DLVALLDVLVSHQHDLSLARALKSPLFGASDDDLVQLVLQRRAMQAQAGEGATVSWWQ
                     VLAQATELPAVLVSAGATLQTWKAWMDTLPPHDALSAIYAHGDVLARFAAATPAPQRE
                     GVLANLRALLNAVLALDGGRYTTPYGLVRALRAGGIAAPVRADTAGVRLLTVHGAKGL
                     EAPLVLMLDTDGEVPRAETMGVLVNWPGEDAYPRRFVFLASESRPPACVVDALAQEQA
                     ERRREELNGLYVALTRTQSTLVISSMEPHAANTGSWWQRLEPLATAVDVDADRGDAFD
                     TAAVEAVDAVCTLKILQNWPLAPVEYAQAALKTIANVSIVEAQTAESVESRIGQAMHR
                     LLEWVEARPGGVPDAPWTAQQCERVATEFTLEAAQVQTATSKALAILRGQGAWAWDAV
                     QLNWAANEVALSRGGRLLRMDRLVQHSITREWWILDYKSSMQPQDQPELVAQLDGYRS
                     VVAQVYPGQVIRAAFLTAHGACIEPFN"
     gene            4397..>5439
                     /locus_tag="PKX82_06515"
     CDS             4397..>5439
                     /locus_tag="PKX82_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HOF51941.1"
                     /translation="MNFSNQVVVIGGGPAGLMAAQVLTDAGEQVHLYDAMPSIGRKFL
                     LAGKGGLNLTHSEGADAFAGRYGARRSVIEALINDFDPQALRTWAQGLGIETFVGSSG
                     RVFPKDMKAAPLLRAWLQRLRHPVSGQGVQFHMRHRWTGWTADGALAFDTPQGPVEVK
                     ARAVVLALGGGSWARLGSNGAWVPLLAARGVAVAPLLPANCGFDVQGGWTEHFSSRFA
                     GQPFKSVAMTFTSAMGAHFARKGEFVATASGVEGSLVYAASSLLRDEIIRTGSATFQL
                     DLLPDMPLERVVAEVAHPRGSRSLSSHLKSRLHIDGIKAAILHERLSKEAFNDPAQLA
                     AAIKALPITLQAAR"
BASE COUNT          912 a         1682 c         1895 g          950 t
ORIGIN      
        1 cgctgcactg gggttgccat cccgcgacgc gctggaagcg ctgatgcatg ccacctggac
       61 gcaggcgttg cagacgccag tgtgtgatgt actgtggcgc accagcgatg acgccggtga
      121 gacgttgctg cccagcagcc tggttcaact gctgcaggcc gcggcaatgg acacgacgca
      181 agggggtatc gatccgcgtc tggagcgcgc ggtgccgact gcgccggtgc atccgccgca
      241 acccgtgggc gctgcgctgc cggtcaagca cctctcggcc agcgcctacg aggacctgcg
      301 ccagtgcccg tatcgctttt ttgcaatgcg gcagttgggt ctgaaaaccg tggacgagct
      361 cgatggggaa gtggacaagc gcgatttcgg attgtggctg catgcggtgc tggcgcagtt
      421 ccatgcggca ctgcagtcag ggcccaccga agacgcaacc gtacgcagcc ggatgctcga
      481 ccagactgca gaagaaatca cccggtcgat ggccttgcct gaaggcgagt ttctaccctt
      541 catggccgcg tggccccagg tgcgcgacgg ctacctgaag tggctgaccg cgcatgaggc
      601 ccagggcgcc cgctttgcca gcggcgaaac ctcccaccgc cagagagtgg gcgatgtgca
      661 actggtgggg cgcatcgatc gtaccgatgc ctttgcagac ggcacggtga tggtgctgga
      721 ctacaagact gagccggttg cgaagtcccg ctcgcgggtc aaggagccgc tggaagacac
      781 gcagattgct ttctatgccg cgctgctgga cgccgacacc ctgcggggcg cctacgtcaa
      841 tgtgggcgag cgcgatggca cagccatgag cgagcaggag cacctggtgg aggcgcgtga
      901 tgccctgttg gaaggcattc agatggacat gcagcgcatt cgtgacggcg cggcattgcc
      961 tgcgctgggg gatgcgggtg cctgcgattt ctgccaggcc cgtagcatgt gccgcaagga
     1021 tttctggagc gttccatgag tgcggtgcat ccgtcccccg cagcggcgca cgcagctttt
     1081 gaacacaacg gcgtggtggt ggcccgtgaa cagttttacg ccatcgcctg cgatccgcgg
     1141 cgcagtgtgg cggtggaggc gtgcgctggt gcaggcaaga cctggatgct ggtgtcgcgc
     1201 atggtgcgtg ccctgctgga cggctgcgcg ccgcaggaca ttctggccat caccttcacc
     1261 aagaaagccg ctggcgagat gcgccagcgc ttgcagcaat ggctggccga gtttgcccag
     1321 gccgatgatg cgacgctgcg cgacgccctg aaggtgcgcg gactggtgca ggacgcgacc
     1381 gatgcgcagc tgcaagacct gcgtgccttg catgccaagc tgttgcgcaa cgggcgcccc
     1441 gtgcagatcc gcactttcca cagctggttt gccgccctgc tgcgcagtgc gccgctggca
     1501 gtgctggacg ccatgggctt gcctacgcaa tacgagctgc tggagaacga tgcccaggca
     1561 gtggcccagg tctggcggcg gtttcagacc cgtgcggtgc aggattccga tgcgcgtgcg
     1621 gattacatgg ctgccgttgc cgcgtatggc cgacaccaga cccacaaggc gctggagtcg
     1681 gcgctgtcca agcgcgtgga gtttgcgctg gctgatgcgc atggtgtggt ggcgtcctcg
     1741 atagcgcact ttgccgagca gttccccgaa ttcgatggac tgacagaccc gctgcagcgg
     1801 gtcgacgcgc ccgctgtgcg tcagcacctc gtcgagactg ccagggcact gggcgcgagt
     1861 accctggtca cctgtaccaa agctgctgtg gcactggagc gcgcgatgac tgacggcgat
     1921 gtaccgtcga tgttcgatgc gctgctgacc caggccggaa agccccgcaa gttctccgac
     1981 aagctggccg gcattgcaat ggtgcaggct gcgcaggaac tactgctgca aattcaggaa
     2041 gcgcgccacc agcacgacgc ctgggtccac cagcagcgca tgacccgcct ggcgcgcggc
     2101 ctgctggaag actacgcagc cctcaagcgc gagcgcggct ggattgacat gggcgatctg
     2161 gagcgggcgg cgctggtgct gatgtcggac ccgcagctct ccggctgggt gcaggaacgg
     2221 ctcgacgccc gggtgcgcca cctgctcatt gacgagttcc aggataccaa cccgctgcaa
     2281 tggcaggcgc tgagtgcctg gttgtcggga tacaccgggg ccggtacggc accgagcgtg
     2341 tttatcgtgg gcgaccccaa acaaagtatt taccgcttcc ggcgtgccga accgcaggtg
     2401 tttcgtgccg ccaaaacctt tgtggtgcag gggctgggcg gcgatatcct gagttgcgac
     2461 cacacgcgcc gcaatgcgcc gtgtgtcatt ggagtggtca attccgtgat gcaggcggcc
     2521 cagcaggcga atgaattcga aggctaccgt gcgcacagca ccgagtccac gcaggaaggg
     2581 caggtgctcg cactgccacg catcctgcgc gaacccaagg cggtcgcagc cagtgcgggg
     2641 gaggaactgg acacagcggc gtggcgagac tcgctgacca cgccccgtgc gcaggccgaa
     2701 gacacccgca agacgctgga atgccggcag gcagcccggt ggctggccca gtggctgcgc
     2761 gacagtgagg gtcgcaagcc cagcgaggtg atggtactgg cgcgcaagcg cgagcggctg
     2821 gggctgatgc aggccgagct ggccgccctg cacatcccgg cccagcagcc cgaaaagacg
     2881 gacctgtgcg acatgccgga agtgcaggac ctggtggccc tgctcgatgt gctggtgtcg
     2941 caccagcacg acctctcgct ggcgcgtgcc cttaaatcgc ccctgtttgg cgccagtgac
     3001 gacgatctgg tgcaactggt actgcagcgg cgtgccatgc aggcacaggc tggggagggc
     3061 gctaccgttt cctggtggca ggtgctggcg caggcaacgg aactgcccgc cgtgctggtg
     3121 tccgcagggg cgaccctgca gacctggaag gcctggatgg atacgctgcc gccccacgat
     3181 gcgctaagcg ccatctacgc ccacggtgat gtgctggccc ggtttgccgc agccacaccg
     3241 gcaccccagc gcgagggcgt gctggccaac ctgcgtgcgt tgctgaacgc ggtgctggcg
     3301 ctggacggcg gccgctacac cacgccctac ggcctggtgc gggccttgcg cgccgggggc
     3361 attgcagcgc ctgtgcgggc cgacacggcc ggtgtgcgtt tgctcacggt gcatggtgcc
     3421 aaggggctgg aggcgccact ggtgctgatg ctcgacaccg atggcgaagt gcctcgcgcc
     3481 gagaccatgg gcgtgctggt gaactggccc ggcgaagatg cgtatccacg ccgcttcgtg
     3541 tttctggcca gcgagtcccg tccgccggcc tgtgtggtgg atgcgctggc gcaggaacag
     3601 gctgagcgcc gtagggaaga actcaacggc ctgtacgtgg cgctgacacg cacccagtcc
     3661 acgctggtga tttccagcat ggagccccat gccgccaata ccggcagctg gtggcagcgg
     3721 ctggagcctt tggccaccgc cgtcgatgtg gatgctgacc gtggcgatgc ttttgacaca
     3781 gcggcggtag aggctgtgga tgcagtctgt accctaaaaa tattgcaaaa ttggcctcta
     3841 gcccccgtgg aatatgcgca agcagctctt aaaacaatag caaatgtttc gatagtagag
     3901 gcgcagactg ccgagtccgt ggagtcccgc attggccagg ccatgcaccg cctgctggag
     3961 tgggtcgagg cgcggcctgg tggtgtgcca gacgcaccct ggacggcaca gcagtgtgag
     4021 cgggtggcta ccgagttcac gctggaggcg gcgcaggtgc agaccgccac cagcaaggcc
     4081 cttgccattc tgcgcggcca gggtgcctgg gcttgggatg ccgtgcagct gaactgggcg
     4141 gccaatgagg tggcgctctc ccgtggcgga cgcctgctgc gcatggaccg gctggtgcag
     4201 cactcgatca cgcgcgaatg gtggatactg gactacaaat cgtccatgca gcctcaggac
     4261 cagcccgagc tggtggcgca actcgatggc taccgcagtg tggtggcaca ggtctacccg
     4321 ggccaggtca tccgcgccgc gttcctcacc gcccatggcg catgcattga acccttcaac
     4381 tgagatttct cccaacatga atttttccaa ccaggtcgtc gtcattggcg gcggccccgc
     4441 cggtctgatg gccgcacagg tattgaccga tgccggtgag caggtgcacc tgtatgacgc
     4501 catgccttcg atcggccgca agtttttgct ggctggcaag ggcgggctca acctcacgca
     4561 ttccgagggt gccgacgcgt ttgcgggccg ctacggcgcc aggcgcagcg tcattgaggc
     4621 gctgatcaac gacttcgatc cgcaggcact gcgcacctgg gcacagggcc tggggatcga
     4681 gacctttgtg gggtcctcgg gccgggtgtt tcccaaggat atgaaagcgg cgccgcttct
     4741 gcgtgcgtgg ctgcagcgtc tgcgccatcc cgtcagcggg cagggcgtgc agttccatat
     4801 gcgccaccgc tggaccgggt ggacggccga tggtgcgctt gcgtttgata ccccgcaggg
     4861 gccggtggag gtcaaggcac gtgccgtggt gctggcgctg ggcgggggta gctgggcccg
     4921 gctggggtcc aacggcgcct gggtgccgtt gctggcggcg cgcggcgtgg ccgtggcgcc
     4981 tttgctgcca gccaattgcg gttttgatgt gcagggcggc tggacagagc atttttccag
     5041 ccgattcgca gggcagcctt tcaaatcggt ggcaatgacg ttcaccagcg cgatgggggc
     5101 gcacttcgcc cgcaagggcg aatttgtagc cacggccagt ggcgtggaag gcagcctggt
     5161 ttacgctgcg tccagcctgc tgcgtgacga aatcatccgc accggcagtg ccacgttcca
     5221 gcttgatctg ttgcccgata tgccgctgga gcgggtggtg gccgaggtgg cccatccgcg
     5281 gggatcgcgg tcgctgtcga gccacctcaa gagccggctg cacattgacg gtatcaaggc
     5341 agccatcctg cacgagcgct tgtccaaaga ggccttcaac gatccggccc agctggccgc
     5401 agccatcaag gcactgccca tcacgctgca ggcggcccg
//
LOCUS       DAOSJM010000147         5393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13593_length_5393_cov_3.930873, whole genome shotgun sequence.
ACCESSION   DAOSJM010000147 DAOSJM010000000
VERSION     DAOSJM010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5393
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13593_length_5393_cov_3.930873"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="ilvB"
                     /locus_tag="PKX82_06520"
     CDS             complement(<1..1262)
                     /gene="ilvB"
                     /locus_tag="PKX82_06520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOF51942.1"
                     /translation="MEISKAEIASATAASQNKSTTASTQELRGAEVLVKALQAEGVKY
                     IWGYPGGSVLHIYDAFYKQDTIQHVLVRHEQAAVHAADGYARATGDVGVALVTSGPGV
                     TNAVTGIATAYMDSIPLVVITGQVPTHAIGLDAFQECDTVGITRPIVKHNFLVKDAND
                     LAETIKKAFHIARSGRPGPVVIDIPKDVSFKKVPYAGYPTSVEMRSYNPVRKGHGGQI
                     RKALQLLLTAKRPYIYTGGGVLLSNASEELRTLVDMLGYPCTNTLMGLGAYPASDRKF
                     LGMLGMHGTVEANNAMQNCDVLLAVGARFDDRVIGNPKHFAQNERKIIHIDIDPSSIS
                     KRVKVDIPIVGDVKDVLGEMIAMIKESAVKPDANALGAWWETIDGWRERNCLKYDRAN
                     KDVIKPQYVVETLWNMTKDADTYITSDVG"
     gene            1560..2126
                     /locus_tag="PKX82_06525"
     CDS             1560..2126
                     /locus_tag="PKX82_06525"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOF51943.1"
                     /translation="MASDLELSEFLKSVEKRAFKRSVYHVRNDEAALDIVQDSMMKLA
                     EHYGHKPIEELPMLFQRILSNCTLDWFRRQKTQNALFSNLSDFESAGDDGDFDLLETL
                     HATTNSDQHESAEKLTERAQTLLAIEVEIQQLPARQREAFLMRYWEEMDVAETAAAMG
                     CSQGSVKTHCSRAVHALGQALRAKGIRL"
     gene            2123..2557
                     /locus_tag="PKX82_06530"
     CDS             2123..2557
                     /locus_tag="PKX82_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3619 family protein"
                     /protein_id="HOF51944.1"
                     /translation="MTTHSLASQQQFEDDFGRAFVARLDDSVDAFPHDISERLKAARM
                     QAISKRKVVKVQVADGHAVSGNVIALQPGGEHHGLWNWLGSLLPLVALVVGILVIDLA
                     QDGIRISEIADVDTELLTDDLPPAAYTDPGFTQYLRSTIQNN"
     gene            2717..3253
                     /locus_tag="PKX82_06535"
     CDS             2717..3253
                     /locus_tag="PKX82_06535"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HOF51945.1"
                     /translation="MTTTVIGAPTWAELNPVQKQSLAPLAGAWNTLSDAHRRKWIALA
                     QNYPNLAPAEQQKLHSRMAEWAALKPKDREQARLNFAETKKIAPTERAANWEAYQALS
                     PEERKKLAAGAKPKPSGAAIVVKPVPVDKLAVVPKVPTAPDIKRPLVTNPQALNRKTL
                     LPIQNAPAPQPPADSAPN"
     gene            3289..3804
                     /locus_tag="PKX82_06540"
     CDS             3289..3804
                     /locus_tag="PKX82_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOF51946.1"
                     /translation="MIANSPVIHPAQNTPGLRRRMACWLYEGILMFGVVFISGYLFGT
                     LSQTRNALDNRNALQAFLFVVFGIYFVWFWSKGQTLAMKTWNIRLVDRNGQAVTQLRA
                     LLRYVLSWLWFLPPLLAVVPFHLPGAEITVIVIGWVAVWALLSRFHPEGQFWHDAWAG
                     TRLISSEPLSR"
     gene            3815..4195
                     /locus_tag="PKX82_06545"
     CDS             3815..4195
                     /locus_tag="PKX82_06545"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOF51947.1"
                     /translation="MKPVNPQKDRTGISRMWYATGYSMAGLRAGWSETAFRQEMLASI
                     VLLPAAFWIGQHWAEVAVLAGTVLLVMIVELLNTAVEAAIDRIGPEWHDLSKRAKDMG
                     SAAVLLSLVLCLGTWSAALYARFA"
     gene            complement(4214..4552)
                     /locus_tag="PKX82_06550"
     CDS             complement(4214..4552)
                     /locus_tag="PKX82_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOF51948.1"
                     /translation="MKQITAIVKPFKLEEVREALAECGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKVEVVVKTEDVDRCVDAIVKAAHTGKIGDGKIFVTSVERVVRI
                     RTGEQDESAV"
     gene            complement(4572..>5393)
                     /locus_tag="PKX82_06555"
     CDS             complement(4572..>5393)
                     /locus_tag="PKX82_06555"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOF51949.1"
                     /translation="VRDYIGKNGFPGVLLGLSGGIDSALVLAIAVDALGPQKVRAIMM
                     PSPYTADISWIDARDMAQRMGVRYDEISIVPQFEAFKASLAQEFAGRPEDTTEENIQA
                     RIRGTLLMALSNKFGSIVLTTGNKSEMATGYCTLYGDMAGGFAVIKDLAKTLVFKLAR
                     WRNAHDPYGTGQSPIPERIITRPPSAELRPDQKDQDSLPPYEVLDAIVERYMENDQGI
                     ESIVADGFAREDVEKVTRLIKINEYKRRQAPVGIRVTHRSFGKDWRYPITSRFRA"
BASE COUNT         1178 a         1575 c         1457 g         1183 t
ORIGIN      
        1 cctacgtccg aagtgatgta cgtatcggcg tctttggtca tgttccagag cgtttccacc
       61 acatattgcg gcttgataac gtctttattg gcacggtcat atttcaggca attgcgttcg
      121 cgccagccgt caatcgtttc ccaccaggca cccagcgcat tggcatcagg cttgaccgca
      181 ctttccttga tcatggcgat catttcgccc agtacatcct tgacgtcacc gacaatcgga
      241 atgtccacct tcacgcgctt ggaaatactg gacggatcaa tatctatgtg aatgatcttg
      301 cgttcgttct gcgcaaagtg tttcgggttg ccaatcacgc ggtcatcaaa ccgggctccc
      361 accgcaagca acacgtcgca gttctgcatg gcattgttgg cttctacggt tccgtgcatg
      421 cccagcatgc ccaggaattt gcggtcactg gcggggtaag cgcccagtcc catgagcgta
      481 ttcgtgcagg gataacccag catatccacc aaagtacgca actcttccga agcgttgctc
      541 agcaacacac caccgcccgt gtaaatatag gggcgcttgg cggtcagcaa gagttgcaag
      601 gccttgcgaa tttggccacc atggcctttg cggacggggt tataagaccg catctccacc
      661 gatgtggggt agcccgcgta cggcaccttt ttgaacgaca catccttggg gatgtcaatc
      721 accacagggc ctggtcggcc actgcgggcg atgtggaatg ccttcttgat cgtctcggcc
      781 aagtcgttgg catctttgac gaggaagttg tgcttgacga tgggacgggt gatacccacg
      841 gtatcgcact cctgaaacgc gtccagtccg atggcatggg taggcacctg gccggtaatc
      901 accaccaagg gaatgctgtc catgtaggcc gttgcaatac cagtgactgc gttggttacg
      961 ccaggcccgg acgttaccag tgcaaccccc acatcacccg tggcgcgcgc atagccatca
     1021 gcagcatgca cagcagcttg ctcatggcgc accagaacgt gttgaatggt gtcctgcttg
     1081 taaaaagcat cgtaaatgtg cagaacggaa ccacccggat agccccagat gtacttgacg
     1141 ccttctgcct gaagcgcttt gactaaaact tcagcgccgc gaagctcttg tgtgctggcg
     1201 gtggtggatt tgttttggga cgcagctgtt gccgaagcga tttcggcttt tgaaatttcc
     1261 atgatgaacc tttgtgaatt tctctgacga aaaaccttgg gtgccccttc gcaaacactt
     1321 gtggcgtcgc gtcgggactt gaggaccaag ccatgggcgc attgaatatt gcgccgaacc
     1381 aggccccgtt tgccttttga tgtgcaaggt gcattatcgc accagatgca ccctgtttca
     1441 cttgaggacc caaaaagtct cagccagggt gcgataatca tcgcgtttcg ggggctacag
     1501 gccgtgtgtc aatgcagcca accaccacat ttgtttgata gcccactgga tttctgcact
     1561 tggcttctga tctcgaactt tctgaattcc tgaagagcgt tgaaaagcgc gctttcaagc
     1621 gttcggttta ccatgttcgc aatgacgaag cagcactgga catcgtccag gacagcatga
     1681 tgaagctggc cgagcattac ggacacaaac cgatcgaaga gctacccatg cttttccagc
     1741 ggattctttc gaattgcacg ctggactggt ttcgccggca aaagacccag aacgctcttt
     1801 tctccaatct gagcgatttc gaatctgctg gtgatgacgg agatttcgat ttactggaaa
     1861 ctttgcatgc caccaccaac tctgaccaac atgagagcgc agaaaagctc actgagcggg
     1921 cacaaacctt gcttgccatt gaggtggaaa tccagcagct accagcacgt caacgggaag
     1981 ccttcctgat gcgttactgg gaggagatgg atgtagcgga aaccgctgcg gccatgggct
     2041 gctcgcaagg cagtgtaaaa acacactgtt ccagggccgt tcatgcgctc gggcaagccc
     2101 tgagggcgaa agggatacga ctatgactac acacagcctg gcttcacaac agcaatttga
     2161 agatgacttc ggccgcgcct ttgtcgcgcg gctggacgat tccgtagacg cctttcccca
     2221 cgacatctcc gaacgtctga aggccgctcg gatgcaggcc atctccaagc gcaaggtggt
     2281 caaggtacaa gtagcagacg gacacgccgt gtctggtaat gtcattgccc tgcaacctgg
     2341 tggcgaacac cacgggctgt ggaactggct tggttcgtta ctgcctctgg tggcgctcgt
     2401 cgtaggaata ctggtgattg acctcgccca ggacggaatc cgcatcagcg aaatcgcaga
     2461 tgttgataca gagttgctga cggacgattt gccgcctgct gcgtataccg accccggatt
     2521 cacacaatat ctgcgttcga cgatccaaaa caattagaaa ttacctgtgc caaagttctc
     2581 tggagtattc tcggttcaac attcactcgc gttgcctgcg aaggtagcag gaggaattgt
     2641 gttggtgata ggcgcgatgg cagtacaagc acagagcaac gccccccact ctccactgaa
     2701 accgttgact cccagcatga ccaccacggt gattggagcc cctacatggg cagagttaaa
     2761 ccccgtgcag aagcaatcgc tggcaccgct ggcaggagcc tggaacacgc tgtctgatgc
     2821 acaccggcgc aagtggattg cgctggcaca aaactatccc aatctggcgc ctgcggagca
     2881 gcaaaaactg cacagtcgca tggcggaatg ggccgcactc aagcctaaag accgcgaaca
     2941 agcccggctg aactttgcgg aaacaaagaa aattgcccct acggagcgcg ctgcaaactg
     3001 ggaggcttac caggcactga gccccgaaga acgcaagaaa ctggctgcag gtgcaaaacc
     3061 caaacccagt ggggcagcaa tagtggtcaa gccggtgcct gtggacaaac tggctgtggt
     3121 tccgaaggta cccaccgctc cagatatcaa gcggccattg gtgaccaatc cgcaagcctt
     3181 gaatcgcaaa acacttttgc caatccagaa cgccccggcg ccccaaccgc ccgcggactc
     3241 agcacctaac tgagcccccc cgcaatggag cctacaatac tgggctccat gattgccaac
     3301 tcccctgtaa tacaccccgc ccagaatact cccggcttgc gccgccgcat ggcgtgctgg
     3361 ctctatgagg gaattttgat gttcggtgta gtcttcatct ccggctacct ctttggcacg
     3421 ctaagtcaga cacgtaacgc actggacaac agaaacgccc tgcaagcttt tctcttcgtt
     3481 gtgttcggta tctactttgt ctggttctgg tccaagggcc agacactggc catgaaaacc
     3541 tggaatatcc gcctggtcga ccgcaacggg caagcagtca cgcaactacg cgccttgctg
     3601 cggtacgtac tgagctggct ctggttcttg ccaccactgc ttgctgtggt gccattccac
     3661 ctgccggggg cagaaatcac cgtcattgtg attggctggg tggctgtctg ggcactgctc
     3721 agccggtttc accccgaggg ccagttctgg cacgacgcct gggcaggcac ccgcctcatt
     3781 agctccgagc cattgagcag gtaaggttgc acatatgaaa cccgtcaacc ctcaaaaaga
     3841 ccgcacgggc atcagccgca tgtggtacgc cactgggtac tcgatggcag gattgcgcgc
     3901 aggttggtcg gagactgcat ttcgccagga gatgctggcc tctatcgttc tactgcctgc
     3961 agccttctgg attggccagc actgggccga agtggcggta ttagccggta cggtcttgct
     4021 ggtgatgatt gtggaactac tcaacacggc agtcgaagca gcgattgacc gcattggccc
     4081 tgaatggcac gacctctcca agcgggccaa ggatatggga agcgctgccg tcctgctcag
     4141 tctggttctt tgcctgggca catggtctgc agcgctctac gcgcgctttg cctgatccct
     4201 ttcggggaaa agatcagacc gccgactcgt cctgctcgcc cgtgcggatt cgcacaacac
     4261 gctcgacgct ggtgacaaaa atcttgccgt caccaatctt gccggtgtgt gcggctttca
     4321 caatggcatc cacacagcga tccacgtctt cggttttgac aaccacttcc accttgacct
     4381 tgggcagaaa gtcgaccaca tactccgcac cgcggtacaa ctcggtatgt cccttttggc
     4441 gaccgaagcc cttgacctca gtgacggtca gaccagtgac tccacattcg gccagtgcct
     4501 cgcgcacttc ttccagcttg aacggtttaa caatggcggt aatctgtttc atctgctagt
     4561 ccaataaaaa atcaggcccg aaagcgactg gtaataggat aacgccaatc cttgccaaag
     4621 ctgcggtggg tcacccgaat tcccacaggc gcctggcggc gtttgtactc gttgattttg
     4681 ataaggcggg tgaccttctc gacatcttcc cgggcgaagc catcggcgac gatgctttcg
     4741 attccctggt cattttccat gtagcgttcc acgatggcat ccagcacctc gtagggcggc
     4801 aaactgtcct ggtctttctg gtcaggacgc aactccgcac tgggcggacg ggtgatgatg
     4861 cgctctggaa tgggactctg gcccgttcca tacgggtcgt gcgcattgcg ccagcgcgcc
     4921 agcttgaaca ccagagtttt ggccagatcc tttatgaccg caaaaccacc tgccatatcg
     4981 ccgtagagcg tgcagtagcc ggtggccatc tccgacttgt ttccggtcgt cagcacgatg
     5041 ctgccgaact tgttggaaag cgccatcagc agcgtgccac ggatgcgggc ctggatgttt
     5101 tcttcggtgg tgtcctccgg tcgcccagca aactcctggg ccaaagacgc cttaaaggcc
     5161 tcgaattggg ggacgatgga aatctcgtcg taccggacac ccatgcgctg cgccatgtca
     5221 cgggcgtcaa tccagctgat atcggcggta tagggcgacg gcatcatgat ggcgcgcacc
     5281 ttctgcggcc ccagtgcatc gaccgcaatc gccagcacca gtgcggagtc aatccctccc
     5341 gacaggccga gcaacacgcc cggaaagccg tttttgccaa tgtagtctcg cac
//
LOCUS       DAOSJM010000148         5347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13731_length_5347_cov_4.895692, whole genome shotgun sequence.
ACCESSION   DAOSJM010000148 DAOSJM010000000
VERSION     DAOSJM010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13731_length_5347_cov_4.895692"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /gene="boxC"
                     /locus_tag="PKX82_06560"
     CDS             complement(<1..1434)
                     /gene="boxC"
                     /locus_tag="PKX82_06560"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HOF51950.1"
                     /translation="MTTASAPRVEYQTSPSQYKHIKLGFNGPVATLAVDIDENAGLRP
                     GYKLKLNSYDLGVDIELNDAIGRIRFEHPEVRTVVVTSAKEKVFCSGANIFMLGVSSH
                     AWKVNFCKFTNETRNGLEDSSKHSGLKFLAAVNGACAGGGYELALACDEILLVDDRSS
                     AVSLPEVPLLGVLPGTGGLTRVTDKRHVRHDLADIFCTSAEGVRGQKAKDWRLVDDIA
                     KPAVFAAKVQERALQLAAQSDRPADGKGVALTPLNRTVEADALRYSHVTVEIDRAKRT
                     ATFTVKAPTGTQPTDVAGIEAAGSNWYPLAMARELEDAILQMRTNELDIGVWLIKTQG
                     DAAAVLAMDATLAAHQSHWLVRETIGLLRRTFSRLDVSSRSLFALIEAGSCFAGTLLE
                     LALACDRSYQLMLPDDAAASPKITVGDTNFGFYPMVTEQSRLQRRFYSEQPALDAVRA
                     QAGKALDADAAFALGLVTSNPDDIDWAD"
     gene            complement(1538..2518)
                     /locus_tag="PKX82_06565"
     CDS             complement(1538..2518)
                     /locus_tag="PKX82_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOF51951.1"
                     /translation="MSSNILHVSGLVPSLCMTTHPTMPEQTAGTEPSAPTPSEKNPFL
                     VSLGERARALRARRGMTRKAVAAAADVSERHLANLEYGEGNASILVLLQVAGALQCSL
                     TELIGDVTTSSPEWLLIRELLEQRDEATLRKARIAIGEALGTGGSDLVANTRVALIGL
                     RGAGKSTLGQMLAEDLGYPFVELSREIEKFAGCSISEIQALYGMNAYRRYERRALEEA
                     IQIYPEAVIATPGGLVSDPATFNQLLSHCTTVWLQADAEDHMKRVSAQGDLRPMAASR
                     EAMEDLKSILSGRAAFYSKAQLRLDTSAQPLEPTFQALRAMVRNLLNLSA"
     gene            2685..4253
                     /locus_tag="PKX82_06570"
     CDS             2685..4253
                     /locus_tag="PKX82_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dehydroadipyl-CoA semialdehyde
                     dehydrogenase"
                     /protein_id="HOF51952.1"
                     /translation="MTELLPNHFNGTWQTGTGQGTVLTDPVLGTELVRVDATGLDLAA
                     GFAFAREQGGQALRALTYVQRAGLLAAAQKVLQANRDAYYDIATANSGTVKNDSAVDI
                     DGGIFTLGTYAKLGEGLGERFFLADGDSARLGKDPLFQSQHVLTPTRGVALFINAFNF
                     PSWGLWEKAAPALLSGVPVIVKPATATAWLTQRMVKDVVDAGIFPAGALSIVCGSSAG
                     LMDQLQPFDVVSFTGSADTAAVIRSHPAVTQRSVRVNIEADSVNSAVLLPGEAAGSEA
                     FDLFVKEVAREMTVKSGQKCTAIRRVLVPEELYAAAAQAISARLAKTTVGNPRNEAVR
                     MGALVSRAQLVAVKDGLEYLKTQAEVLHDGSAAALVDADANIACCVGPTLLGVRNAAG
                     SDSADRIHDTEVFGPVATLVPYRTTGGSMDHAFALVRRGQGSLVASLYGSNPQALAHA
                     AAELADSHGRVHIISPDVAALQTGHGNVMPQSLHGGPGRAGGGEELGGLRALNFYHRR
                     TAIQGSTTVLAQLQ"
     gene            4269..>5347
                     /locus_tag="PKX82_06575"
     CDS             4269..>5347
                     /locus_tag="PKX82_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HOF51953.1"
                     /translation="MPLPEHIDAPGDTFNFAQHLLTVNTQRPAKAAFIDDGGALTYGA
                     LDERVRRVAAGLRALGIKREERVLLLMQDCNDWPVSFLGAIYAGIVPVAVNTLLTADD
                     YAYMLEHSRAQAVLVSGALLPAVTAAMTKSDHEVHKVIVSHPAAPLHPAEVEFESFVQ
                     GNAPLAKAAATSPDDPCFWLYSSGSTGRPKGTVHSHANPYWTIELYGKGILELRESDV
                     CFSAAKLFFAYGLGNALTFPMGVGATTLLMAERPTPDATFKRWLGGVGGVKPTVFYGA
                     PTGFAGMLASPNLPAKTEVAMRLVSSAGEALPAEIGERFQRHFGVDIVDGIGSTEMLH
                     IFLSNLPGKVRYGTTGWPVPGYDIEL"
BASE COUNT          926 a         1650 c         1755 g         1016 t
ORIGIN      
        1 atcagcccag tcgatgtcgt cggggttgga ggtgaccaga cccagtgcaa aagcggcgtc
       61 tgcgtcgagt gccttgccag cctgggcgcg caccgcgtcc agcgcaggct gttcgctgta
      121 gaagcggcgc tgcagacggc tttgctcggt gaccatgggg tagaagccaa agttggtgtc
      181 gcccaccgta atcttgggcg aagcggctgc gtcgtcgggc aacatgagtt ggtagctgcg
      241 gtcgcaggcc agggccagtt ccagcagggt gcccgcaaag caggagccgg cttcgatcaa
      301 ggcaaacaga ctgcgggaag acacgtccag gcggctgaag gtgcggcgca gcaggccgat
      361 ggtttcgcgc accagccagt ggctctggtg ggctgccagg gtggcgtcca tggccaacac
      421 cgcagcggcg tcgccctggg tcttgatcag ccagacgcca atgtcgagct cgttggtgcg
      481 catttgcaga atggcgtctt ccagttcgcg tgccatggcc agcgggtacc agttgctacc
      541 tgcggcttca atgccagcca catcggtggg ctgcgtgccg gtgggggcct tcacggtgaa
      601 agtggcggtg cgcttggcgc ggtcaatctc aaccgtgacg tggctgtaac gcagggcatc
      661 ggcttcgaca gtgcggttca agggtgtgag ggctacgcct ttgccatctg cggggcggtc
      721 gctctgggcg gccagttgca gggcacgttc ctgcaccttg gcggcaaaca ctgcgggctt
      781 ggcaatgtcg tccaccagac gccagtcttt ggccttctgg ccgcgcacgc cttcggcact
      841 ggtgcagaag atgtcggcca ggtcatggcg cacatggcgc ttgtcggtga cgcgggtcag
      901 tccgccggtg ccgggcagca cgcccagcaa aggcacttcg ggcaggctca ccgcgctgga
      961 gcggtcgtcc accagcagga tttcgtcgca ggccagggcc agctcgtagc ccccgccagc
     1021 gcaggcgccg ttcaccgcgg ccaggaattt caggccgctg tgcttggagg agtcttccag
     1081 gccgttgcgg gtttcgttgg tgaacttgca gaagttcact ttccaggcgt ggctggaaac
     1141 gcccagcatg aagatgttgg cgccggagca gaacactttc tccttggcgc tggtgaccac
     1201 gaccgtgcgc acttcgggat gttcaaagcg gatgcggccg atggcgtcgt tgagttcaat
     1261 gtccacgccc aggtcgtagc tgttgagctt gagtttgtag ccagggcgca ggccggcgtt
     1321 ctcgtcgatg tccacggcca aggtggccac cgggccgtta aagcccagtt tgatgtgttt
     1381 gtactgggac gggctggtct ggtattcaac gcggggtgca gaggcggtag tcatggggct
     1441 cctcacaggg atgtaaaaaa atgcagttgt ttggatagat gcacgaataa tagtgcatgt
     1501 atttaatcca agtcaagcac tttagttcac atcgatttca cgcagagagg ttcagcaggt
     1561 tgcgcaccat ggcgcgcagt gcctgaaagg tgggctccag gggctgggcg ctggtatcga
     1621 gccgcagctg tgccttggaa tagaaggccg cacggccaga cagaatgctt ttgaggtctt
     1681 ccatggcctc ccggctggcc gccatggggc gcagatcgcc ctgggcggac acgcgcttca
     1741 tgtggtcctc ggcatcggcc tgcagccaga cggtggtgca gtggctcagc agctggttga
     1801 acgtggccgg gtcggatacc aggccgccgg gcgtcgcgat cacggcttcc gggtaaatct
     1861 ggatcgcctc ttccagtgcg cgccgctcgt agcggcggta ggcgttcatg ccatacagcg
     1921 cctggatctc ggaaatgctg cagccagcga acttctcgat ctcccggctc aactccacaa
     1981 acgggtatcc gaggtcctcc gccagcattt gccccagcgt ggacttgccg gcaccccgca
     2041 agccaatgag ggccacgcgc gtgttggcca ccaggtcgct gccaccggtg cccagcgcct
     2101 cgccgatggc aatgcgcgcc ttgcgcaagg tggcttcgtc acgctgctcc agcaattcgc
     2161 ggatcaacag ccattcgggc gacgacgtcg tgacgtcgcc gatcagttcg gtgagcgagc
     2221 actgcaaggc gcctgccacc tgcagcagga ccaggatgga ggcattgcct tctccgtact
     2281 ccagattggc caggtggcgc tcggacacat cggccgccgc ggcgacggcc ttgcgtgtca
     2341 ttccccggcg cgcccggagt gcgcgggcgc gttcgcccag agaaaccagg aacggatttt
     2401 tctcgctggg ggtcggtgcg gatggctctg tgcccgctgt ttgttctggc atcgttggat
     2461 gtgttgtcat gcaaagactc ggtactaacc ctgatacatg caatatattg cttgacatgg
     2521 ggtcgactgc actttatggt tgatggacgc acaataattc ataaccaggc ggttggcaag
     2581 acctgccact gcatggagac cgccaccgtc acccagggcc gctcgctggt tctgtacctt
     2641 ctgccgcaag gcagcattga attcacccaa taaggaaagc caccatgacc gaactacttc
     2701 ccaaccattt caatggcacc tggcaaaccg gtacaggaca gggaacggtg ctgaccgacc
     2761 cggttctggg gactgaactg gtgcgcgtgg atgccaccgg tctggacctg gcagcgggct
     2821 ttgcctttgc ccgcgaacag ggcggccagg ccttgcgggc cttgacctat gtgcagcgcg
     2881 cgggtctgct ggcggcagcc cagaaagtcc tgcaggccaa ccgcgatgcg tactacgaca
     2941 ttgccacagc caacagcgga accgtcaaga acgactcggc agtagatatt gacgggggca
     3001 tcttcactct gggcacctac gccaagctcg gtgaaggtct gggtgagcgc ttttttctgg
     3061 ccgatggcga cagtgcgcgt ctgggcaagg accctctgtt ccagtcgcaa cacgtcctta
     3121 cgcccacgcg tggcgtggcg ctgttcatca atgccttcaa tttcccgtca tggggactgt
     3181 gggaaaaggc ggcgcccgcc ctgctttcgg gtgtgcccgt gatcgtcaag cctgccaccg
     3241 ccaccgcctg gctgacacag cgcatggtga aagatgtggt cgatgcgggc atcttccctg
     3301 caggtgcgct gtccattgtg tgcggcagtt cggccggcct gatggaccag ctgcagcctt
     3361 tcgatgtggt gtccttcact ggctcggcgg ataccgccgc agtcattcgt tcccacccgg
     3421 ccgtcaccca gcgttcggtg cgtgtgaaca tcgaggccga cagtgtcaac tccgccgtat
     3481 tgctgcccgg cgaggcggcc ggcagcgagg cctttgacct gttcgtcaag gaagtggcgc
     3541 gcgagatgac ggtcaagtcc ggccagaaat gcaccgccat ccgccgcgtg ctggtgcccg
     3601 aagagctcta tgccgctgcg gcacaagcca tttccgcccg cctcgccaag accaccgtag
     3661 gcaacccacg caatgaggcg gtgcgcatgg gcgcactggt cagccgggca caactggtgg
     3721 cagtcaagga cggcctggag tacctgaaga cacaggccga ggtgctgcat gacggtagcg
     3781 ccgcggcgct ggtggatgct gatgcgaaca ttgcctgctg tgtgggcccc acgctgctcg
     3841 gtgtgcgcaa cgccgctggc agcgacagcg ctgaccgcat ccatgacacc gaagtctttg
     3901 gtcccgtggc cacactggtg ccgtaccgca ccaccggggg cagcatggac cacgcgtttg
     3961 cgctggtgcg ccgcggccag ggttcgctgg tggcgtcttt atatggcagc aacccgcagg
     4021 cgctggcgca tgcggcagcc gagctggccg acagccatgg ccgcgtgcac atcatttccc
     4081 ccgacgtggc cgcgctgcag accggacacg gcaacgtcat gccgcagtcc ctgcacggcg
     4141 gccccggccg ggccggtggt ggtgaagaac tgggcggctt gcgcgcactg aacttctacc
     4201 accgccgcac cgccatacag ggcagcacca ccgtgctggc gcagttgcaa taacccgcgg
     4261 agacgagcat gcccctaccc gaacacatcg atgcgcctgg cgatactttc aatttcgcgc
     4321 agcacctgct gacggtcaat acgcagcgtc cggcgaaggc ggcgttcatt gatgatgggg
     4381 gcgccctcac ctacggcgcg ctggacgaac gggtgcgccg tgtggcagcg ggtcttcgtg
     4441 cgctgggtat caagcgcgaa gagcgtgtgc tcctgctgat gcaggactgc aacgactggc
     4501 cggtcagctt tctgggcgcc atctacgccg gcatcgtgcc ggtggcagtg aatacgctgt
     4561 tgacggcaga cgactacgcc tacatgctgg agcattcacg cgcccaggcg gtgctggttt
     4621 cgggtgccct gctccccgca gtgacggcgg ccatgaccaa gtcagaccac gaggtacaca
     4681 aggtcatcgt ttcacacccc gcggcgccac tgcatccggc cgaagtggag ttcgaaagtt
     4741 ttgtgcaggg caacgcgccg ctggccaagg cggccgccac cagcccggat gacccctgtt
     4801 tctggctgta ttcctcgggc tccacggggc ggcccaaagg cacggtgcat tcgcacgcca
     4861 acccctactg gacgatcgag ctgtatggca agggcattct ggaattgcgc gagagcgatg
     4921 tgtgcttctc ggcggccaag ctgttctttg cctatggtct gggcaatgcg ctgaccttcc
     4981 ccatgggggt cggggccacc accctgctga tggcggagcg ccccacaccc gacgccacct
     5041 tcaagcgctg gctgggcggc gtgggcggag tcaagcccac cgtgttttat ggcgcaccca
     5101 ccggttttgc cggcatgctg gcctccccga atctgccggc caaaaccgag gtggcaatgc
     5161 gcctggtttc atcggctggt gaggcgctgc cggccgaaat cggcgaacgc tttcagcgcc
     5221 actttggcgt ggacattgtg gatggcattg ggtccaccga gatgctgcac atcttcctgt
     5281 ccaacctgcc gggcaaggta cgctacggca ccacgggctg gccggtgccg ggctacgaca
     5341 ttgaact
//
LOCUS       DAOSJM010000149         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13740_length_5345_cov_4.083176, whole genome shotgun sequence.
ACCESSION   DAOSJM010000149 DAOSJM010000000
VERSION     DAOSJM010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13740_length_5345_cov_4.083176"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            185..904
                     /gene="radC"
                     /locus_tag="PKX82_06580"
     CDS             185..904
                     /gene="radC"
                     /locus_tag="PKX82_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOF51954.1"
                     /translation="MPLKDLPPDARPREKLLARGAGALSDAELLALLLRTGIQGKGVL
                     QMAQELLQLKNHSTTGTTDGCQTGFGGIAGLLNATADDLKRVKGLGPAKRAEIVAVLE
                     LARRAMAQQLTERTVLADPGAVKHYLQLHLASRGHEVFAVLFLDSQNKLLALEELFRG
                     TLTQTSVYPREVVIRALHHQAAAVVLAHNHPSGTVQPSRADEALTQALKAALALVDVR
                     VLDHIIVAPGLALSMAEKGLV"
     gene            901..1545
                     /locus_tag="PKX82_06585"
     CDS             901..1545
                     /locus_tag="PKX82_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOF51955.1"
                     /translation="MKVTSLADLKLVKKQIEAQAQREAAEAAQRALEAKRQAAQRNLF
                     QAAVGAVNVLPDKRRAHLLPTPPAPIPKQKQADEAAALQEAISDEVDVSSLLDTDERL
                     SFRRPGVGPDVTQKLRRGNWSIQRQVDLHGLRTEEAREALSTFIRDAHKQGIRCVRVV
                     TGKGLGSPGKTPVLKDKVHRWLVQKSEVLAFVQAQPAQGGAGALVVLVQPLRLS"
     gene            1551..2315
                     /gene="modA"
                     /locus_tag="PKX82_06590"
     CDS             1551..2315
                     /gene="modA"
                     /locus_tag="PKX82_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF51956.1"
                     /translation="MVSTTKRLLQRSGVLISLLVALPSFAGQVQVAVAANFAEPIKAI
                     AAVLEKTTGHQVQVTVGATGKLYAQIKNGAPFDVLLAADTKTPEALEKEGHALPGSRF
                     TYATGKLVLWSADARKVDAKGEVLKSAGLRKVAYASPKVAPYGAAAVEAIDRLGLTAS
                     LAPKLVQGESIGQAFSFVYTGNAEVGFVALSQVLAGGKLKSGSLWIVPKALYSPIQQD
                     AVVLQRAAGNEAAQALVTLLKSPNIQDLIRSYGYDI"
     gene            2329..3021
                     /gene="modB"
                     /locus_tag="PKX82_06595"
     CDS             2329..3021
                     /gene="modB"
                     /locus_tag="PKX82_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOF51957.1"
                     /translation="MFLTASDLQAIWLTLQVATLTTAILALVGTPIAWWLARTRSWLK
                     TPVSALVALPIVLPPSVLGFYLLVAMGPHGPVGQWTAAWGLEPLVFTFPGLVVASVFY
                     SLPFMVQPVHNAMEAMGTRPLEVAASLRASPLDTFFNVVLPLCKPGLITGAIMSFAHT
                     VGEFGVVLMVGGNIPGVTRVVSVQIFDHVEAMEYGDAHRLAAVMLGFAFVVLMALNLY
                     NQRTSRRAQAIQ"
     gene            3018..4136
                     /gene="modC"
                     /locus_tag="PKX82_06600"
     CDS             3018..4136
                     /gene="modC"
                     /locus_tag="PKX82_06600"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HOF51958.1"
                     /translation="MNTPAHTAQPLEVQARLQRTGFALDVHLQLPPQGVTVLFGPSGS
                     GKTTCLRVLAGLEREATGRVCVDGEVWQDSTHGVFRPVHQRALGYVFQEASLFAHLNV
                     QANLQFGFQRTPAAQRRHRWDQGLELLGIGHLLQRMPHELSGGERQRVAIARALASSP
                     RVLLLDEPLAALDAQRKSEILPWLEQLHDQLDIPVVYVTHSPDEMARLADHLVLLDAG
                     TVRASGPVMDLMTRLDLPLLQGDAAAALIEARVCGLDPTEVMCILEFDGGRLLLPQTR
                     AVPLPADTRVRVRIQARDVSLSLQQPEQTSVLNILPATVVEITDDGPGQVLVALQIGH
                     GTPGSRLLSRISQLSARRLGITPGQAVYAQIKGVAMVR"
     gene            complement(4137..>5345)
                     /locus_tag="PKX82_06605"
     CDS             complement(4137..>5345)
                     /locus_tag="PKX82_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOF51959.1"
                     /translation="FLESNPIPVKYAMAMLGLRAQDLSARQLSDVIAAPYGQLLARNH
                     RRPGQPMPLHLHGGALVYGVLQTPQTVELRSTATEVGAAPADAFSRLDSGDTRWRSAA
                     DKVRRVLNKPIPVLIQGESGVGKELFARASHDSGPRRSGPFVAINCAAIPENLIEAEL
                     FGYAPGAFTGARREGSPGRLREAHGGTLFLDEIGDMPLAMQARLLRVLQERQVTPLGG
                     GKPVEVDFALVCASHRTLREEASAGRFRSDLYYRINGLTVVLPPLREREDFSALTLRM
                     LTDLNPGREVRVDPTLLERLQRYPWPGNVRQYANVLRTASAMLDEGEDSIGWSHLPDD
                     VLQDLEQAAVAPPTRVQLPVAVAAGGDTLDALSRAAIRQAIDRAAGNMSQAARSLGIS
                     RQTLYRKLNG"
BASE COUNT          955 a         1792 c         1721 g          877 t
ORIGIN      
        1 attcgatgaa ttctggggtt tctatgacgg tgtacaccaa ttaattgtaa caacaaataa
       61 tagtaaactt ttgaggtgac acgattttta ttgcatgccc ccctctgggc gctgaaaacg
      121 gggactcgct taaactgacc tcctagctga cagtagccgc cagcggcgca actttcctgc
      181 atatatgccc ctcaaagacc tgcccccgga cgcccgaccg cgcgagaagc tgctcgcgcg
      241 cggcgctggc gcgctgagtg acgccgaact gctggccctg ctgctgcgca ccggcatcca
      301 gggcaagggc gtgctgcaga tggcacagga gctgctgcaa ctcaaaaacc atagcactac
      361 aggcacaacg gacgggtgcc agacgggatt tggaggcata gcaggccttc tcaacgccac
      421 ggcggacgac ctcaagcgcg tcaagggcct gggcccggcc aagcgcgccg agatcgtggc
      481 agtgctcgaa cttgcccgcc gcgccatggc ccagcaactg accgagcgca ccgtactggc
      541 cgaccccggc gcggtgaagc actacctgca gctgcacctg gcctcgcgtg ggcatgaagt
      601 gtttgccgta ctgtttctgg actcccagaa caaattgctg gcactggaag aactgttccg
      661 cggcacgctg acccaaacgt cggtctaccc ccgcgaggtg gtgatccgcg ccctgcacca
      721 ccaggcggcc gccgtggtgc tggcccacaa ccaccccagt ggcacggtgc agcccagccg
      781 cgccgacgaa gcgctcaccc aggccctcaa agcggcgctg gcgctggtgg atgtgcgtgt
      841 gctcgaccac atcatcgtgg cacccggact ggccctgtcc atggccgaga aagggctggt
      901 atgaaagtca cgtccctggc cgacctcaag ctggtcaaaa agcagatcga ggcacaggcc
      961 cagcgcgaag cggccgaagc cgcccagcgg gcgctggagg ccaagcgcca ggccgcgcag
     1021 cgcaacctgt ttcaggcggc agtcggcgcc gtcaacgtgc tgcccgacaa gcgccgcgcc
     1081 catctgctac ccacaccgcc ggccccgatc ccaaagcaga agcaggccga cgaagctgct
     1141 gccttgcagg aagccatcag cgacgaagtc gatgtcagca gcctgctcga caccgacgag
     1201 cgcctgagct ttcgccgccc gggggtaggc cccgatgtca cgcagaaact gcgccgtggc
     1261 aactggtcga tccagcgcca ggtggacctg catgggctgc gcaccgaaga ggccagagag
     1321 gccctttcca ccttcatccg cgatgcccac aaacagggca tacgctgcgt gcgcgtggtc
     1381 acgggcaaag gattgggctc acccggcaag acgccggtgc tcaaggacaa ggtgcaccgc
     1441 tggctggtcc agaagtccga ggtactggct tttgtccagg cgcagcccgc gcagggcggt
     1501 gccggcgcgc tggtggtgtt agtgcagcca ttgcgattaa gctagacgcc atggtatcga
     1561 cgacaaaacg attgttgcaa agaagtggcg tgctgatcag cctgctggtg gcattgccca
     1621 gctttgccgg acaagtgcag gtggcggtgg cggccaactt cgctgagccc atcaaggcca
     1681 tcgcggccgt gctggaaaag accaccggcc accaggtaca ggtgactgtg ggcgccaccg
     1741 gaaagctcta cgcacagatc aaaaacggcg caccgtttga cgtactgctg gccgctgaca
     1801 ccaaaacccc cgaagcactg gaaaaagaag gccatgccct gcccggcagc cgcttcactt
     1861 acgccaccgg caaactggtg ctgtggtcgg ccgatgcccg caaagtggat gccaaaggcg
     1921 aggtactcaa aagtgcgggc ctgcgcaaag tggcctacgc ctcgcccaag gtcgcgccct
     1981 acggcgccgc cgcagtcgaa gccatcgacc gactgggtct gaccgcctcg cttgcaccca
     2041 agctggtgca gggcgagagc atcgggcagg cattcagctt cgtctacaca ggcaacgccg
     2101 aggtgggctt tgtcgccctg tcgcaggtgc tcgcgggcgg caagctcaaa agcggctccc
     2161 tgtggatcgt gcccaaagcg ctgtacagcc cgatccaaca agacgccgtg gtgctgcaac
     2221 gcgcagcggg caacgaggcc gcgcaggcac tcgtcacact gctgaaaagc cccaacatcc
     2281 aagatctgat ccgttcttac ggctacgaca tctgatctgc gcgttcccat gttcctgacc
     2341 gcatccgacc tgcaggccat ctggctcacg ctccaggtcg ccacgctcac caccgccatc
     2401 ctcgcactcg tgggcacgcc catcgcctgg tggctggcac gcacacggtc ctggctgaag
     2461 accccggtga gcgcgctggt cgcattgcca atcgtgctac cgccatcggt gctggggttc
     2521 tatctgctgg tggccatggg cccccatgga ccggtggggc agtggactgc ggcgtggggg
     2581 ctggagccgc tggtgtttac tttccccggt ctggtcgtgg catcggtttt ttactcgctg
     2641 ccgttcatgg tgcagccggt gcacaacgcc atggaagcca tgggcacccg cccgctggag
     2701 gtcgctgcca gcctgcgggc gtcgccgctc gacacgtttt tcaacgtggt gcttcccctg
     2761 tgcaaacccg gtctcattac tggcgcaatc atgagctttg cccacacggt gggggagttt
     2821 ggcgtggtac tgatggtggg cggcaatatt ccgggcgtga cacgtgtggt gtcggtgcag
     2881 atattcgacc acgtggaggc catggagtac ggcgatgcac accgcctggc agccgtcatg
     2941 ctgggctttg cgttcgtcgt gctgatggca ctgaacctct acaaccagcg cacctcccgg
     3001 cgcgcacagg ccatccagtg aacacccccg ctcacaccgc acagccgctg gaagtacagg
     3061 cccgactgca acgcacgggc tttgcgctgg acgtccatct gcagctgccg ccccagggcg
     3121 tgaccgtgct cttcggcccc tcgggctcgg gcaagaccac ctgcctgcgc gtgctcgccg
     3181 ggctggagcg cgaagccact ggccgcgtct gcgtggatgg cgaagtctgg caggacagca
     3241 cgcacggcgt gtttcgcccg gtgcaccagc gcgcactggg ctatgtgttt caggaagcca
     3301 gcctgtttgc ccacctgaat gtgcaggcca acctgcagtt cggtttccag cgcacccccg
     3361 cagcgcagcg tcggcaccgc tgggaccagg gcctggagtt actgggcatt ggccatctgc
     3421 tgcagcgcat gccgcatgaa ctctcgggtg gcgaacgcca gcgtgtggcc atcgcgcgtg
     3481 cactggccag cagcccgcgc gtgctgttgc tggacgaacc actcgccgca ctggacgcgc
     3541 agcgcaagtc cgaaatactg ccctggctgg agcagctgca cgaccagctg gacattcccg
     3601 tcgtgtatgt cacgcactcc cccgacgaga tggcccgtct ggccgaccat ctggtgctgc
     3661 tggatgcggg cacggtgcgc gccagcgggc cggtcatgga cctgatgacg cggctggatc
     3721 tgcccctgct gcaaggggat gcggcggcgg cgctgatcga agcacgcgtg tgcggccttg
     3781 accccaccga agtgatgtgc attctggagt ttgacggcgg gcgcctgctg ctgccgcaga
     3841 cccgcgccgt gcccttgccc gccgacacgc gggtacgcgt gcgcattcag gcccgcgatg
     3901 tgagcctgtc cctgcaacaa cccgagcaga ccagtgtgct caacatcctg cccgccaccg
     3961 tggtggagat caccgacgat ggccccggcc aggtgctggt ggcactgcag ataggccatg
     4021 ggaccccggg gtcgcgcctg ctatcgcgca tcagccagct ctcggcacgg cggctgggca
     4081 tcacaccggg ccaggccgtc tacgcccaga tcaagggcgt ggcgatggtg cgttagtcaa
     4141 ccattgagct tgcggtacag cgtctggcgg ctgatgccca ggctgcgggc agcctgcgac
     4201 atattgcccg cggcgcggtc aatcgcctgg cggatggcgg cccgcgagag cgcatccagc
     4261 gtatccccgc ccgcagccac agccaccggc agttgcaccc gggtgggtgg tgccaccgcg
     4321 gcctgctcca ggtcctgcaa cacatcgtca ggcaaatggg accagccaat gctgtcctcg
     4381 ccttcatcca gcatggcgct ggcggtgcgc agcacattgg cgtactggcg cacgttgccg
     4441 ggccagggat agcgctgcag ccgctccagt agggtgggat cgacccgcac ctcgcgtccg
     4501 ggattcaggt cggtcagcat gcgcagggta agcgccgaaa aatcttcgcg ctcgcgcagg
     4561 ggaggcaaca ccacggtgag gccattgatg cggtagtaca ggtcgctgcg gaagcgcccg
     4621 gccgaggcct cctcccgcaa ggtgcggtgg ctggcgcaca ccagcgcgaa gtccacctcc
     4681 accggcttgc cgccccccag tggcgtgacc tggcgctcct gcagcacgcg cagcaggcgg
     4741 gcctgcatgg ccagcggcat gtcgccaatc tcatcgagaa acagcgtgcc accatgggct
     4801 tcgcgcaggc gccccgggct gccctcgcgc cgtgcacccg tgaaggcgcc cggggcgtag
     4861 ccgaacagct cggcttcaat gaggttctca ggaattgcgg cgcagttgat cgccacaaaa
     4921 ggaccgctgc ggcgtggccc gctgtcgtgg gacgcgcgcg caaacaactc cttgcccacg
     4981 cccgactcgc cctggatcag taccgggatc ggcttgttca gcacgcggcg caccttgtcg
     5041 gcagcgctgc gccagcgggt gtcgccactg tcgaggcgac tgaaagcatc ggcaggcgct
     5101 gcaccgactt cagtcgcagt ggaacgcagc tccaccgtct ggggcgtttg cagcactcca
     5161 tacaccagcg caccaccgtg cagatgcaac ggcatcggct ggcccgggcg gcggtggttg
     5221 cgggccagca gctggccgta gggcgctgcg atcacatcgg agagctggcg tgcgctgagg
     5281 tcctgcgcgc gcagccccag catcgccatg gcgtacttca ccgggatcgg gttcgattcg
     5341 aggaa
//
LOCUS       DAOSJM010000150         5310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13870_length_5310_cov_4.357564, whole genome shotgun sequence.
ACCESSION   DAOSJM010000150 DAOSJM010000000
VERSION     DAOSJM010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13870_length_5310_cov_4.357564"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PKX82_06610"
     CDS             <1..753
                     /locus_tag="PKX82_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit beta"
                     /protein_id="HOF51960.1"
                     /translation="VIFLVYNDHATSFDLGVIPTFAIGTAAEFEPADEGYGPRPVPTV
                     VGHPELASHIAQSVIQQDFDLTIVNKMDVDHGLTVPLSLMCGELDPVKDAWPCPVIPF
                     AVNVVQYPVPSGQRCFNLGKAIRKAVESYDEDLNVHIWGTGGMSHQLQGARAGLINRT
                     WDNEWLDKMIADPAAAAAVPHIDYVREAGSEGIELVMWLIARGAMSDVPYNDKDATDL
                     IAARAHSVRARHRFYHVPASNTAVGHLILENV"
     gene            755..1717
                     /locus_tag="PKX82_06615"
     CDS             755..1717
                     /locus_tag="PKX82_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOF51961.1"
                     /translation="MSKTIKVALAGAGAFGIKHLDGIKNIDGVEVVSLISRDLEKTKE
                     VADKYGIGHVTTDLADSLALPEVDAVILCTPTQMHAEQTIACLKAGKHVQVEIPLADS
                     LKGAEAVVAMQKQTGLVAMCGHTRRFNPSHQYVNQQITAGKFNIQQMDVQTYFFRRTN
                     TNALGQARSWTDHLLWHHAAHTVDLFAYQANSPIVQANAIQGPIHPVLGIAMDMSIQL
                     RAANGAICTLSLSFNNDGPLGTYFRYIGDTATYLARYDDLYTGKEEKIDVSQVAVSMN
                     GIELQDREFFAAIREGREPNSSVGKVFACYQVLHQLEQQLAAQR"
     gene            1908..3806
                     /locus_tag="PKX82_06620"
     CDS             1908..3806
                     /locus_tag="PKX82_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF51962.1"
                     /translation="MTEQEFLGEMPVVISVSRLAQRLDETPGAVTILDRDFIRMSGAR
                     DVADLLRVVPGFQTTNSFETDAPIVTYHGRVDDWANRVQVMVDGRSVYSGLLQGSAGL
                     GLETLALADIERIEVLRGSNSAAYGARAFLGVINIVSRDPRQTFGTQAQATVGQNGVA
                     DLGGSVGWGEDSAVFRVSVDSRGDDGLRKTGSAAGSSGKSRVNRANLAGALSLGSATE
                     LSLHAGLLDIAAYRGTLIGDVGNPQRMRFMGSGYVQGEWARALSADEDLKVELSYTRI
                     ENKDSYIYQSDGPAPGTYRGIRISLDAVEQNAAASLQYIRRYSNDVRMVLGTELRSER
                     IDSRSEFDRAGNVETDFTRIFGNLEWRIHPQWLLNAGAMLEDSNAVDATLAPRWMLNW
                     TPLRGHTFRAGSSTAFRTPSAYENFADSAFYDINGQNRLSAVVGSGQLRPERIVAREL
                     GYLFYLAGRTLTGDVRLFDEHISDGIATTASTPTAFVNSEDYRITGGELDLVWRPHPA
                     VRVGLSHAAAEVQINQSPNLYPTAFTTQYAVPKTSDALSLMLNLPGGYSASLIYSQAE
                     RLALMSAVGKTINIERTDVRLAKNFRLGKSKAELALTVQNLNNPYPDGDRKFYFDSRA
                     MVTLRVEN"
     gene            3819..4730
                     /locus_tag="PKX82_06625"
     CDS             3819..4730
                     /locus_tag="PKX82_06625"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HOF51963.1"
                     /translation="MHHFVRFLLLVSLTLLSGGARAIDVVVVLSERGPGYLEATEVMV
                     AELGRAGLTVSDTDVRSANSLATALAQQTPRVIVTLGYESLRQVLEARPRVPVLAALI
                     PRSGFERLLRDHASTRPAVAALYLDQPIGRQMDVIRLAFGAGHRVAVLLGPESSWQRS
                     LLTASAGAQGFSLQLAEVGASQTVAHALADVLDGADVLLALPDTQVYNATTVGNVLMS
                     SYRARVPVLAFSPAYVRAGATLSLHSTPVQVALMVRQHLQAGAVLTNQYPADFAITVN
                     ERVARSLDLDLNAAMLTEQLKRLARKP"
     gene            4727..>5310
                     /locus_tag="PKX82_06630"
     CDS             4727..>5310
                     /locus_tag="PKX82_06630"
                     /inference="COORDINATES: protein motif:HMM:NF021499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51964.1"
                     /translation="MSGGSIKSRMLAVAILPVTLVVIGVVLVFWLGRSQDMDEAHAQR
                     SRLLARQVALASELGVFSGDVATLQGIVNGVLREPDVISAAVFDTAGVAMVSAGEVRN
                     VTMAQMLDPFTLQQRQRLQIDLLKEPVVAAALPMDHWFAPAAAQRTSKVLGYVILEVS
                     RASLVQQELRILWLALLVGSVGILGGGLLAFYMGR"
BASE COUNT          960 a         1564 c         1707 g         1079 t
ORIGIN      
        1 gtgatttttc tggtctacaa cgaccatgcc accagcttcg acctgggcgt gatccccacc
       61 ttcgccatcg gcaccgcggc cgagttcgaa cccgccgacg aaggttacgg cccgcgtccg
      121 gtgcccaccg tggtgggcca ccccgagctg gcctcgcaca tcgcgcagtc ggtgatccag
      181 caggactttg acctgaccat cgtcaacaag atggatgtgg accacggtct gaccgtcccg
      241 ctgtccctga tgtgcggcga gttggacccg gtcaaggatg cctggccctg cccggtcatt
      301 ccgtttgcgg tcaacgtggt gcagtacccg gtgcccagcg gccagcgctg cttcaatttg
      361 ggcaaggcca tccgcaaggc ggtggagagc tatgacgaag acctgaacgt gcacatctgg
      421 ggcaccggtg gcatgagcca ccagctgcag ggtgcgcgcg ccggcctgat caaccgcacc
      481 tgggacaacg agtggctcga caagatgatt gccgaccctg cggcggccgc ggcggttccg
      541 cacatcgact atgtgcgtga agctggcagt gaaggcattg aactggtgat gtggctgatc
      601 gcccgcggcg cgatgtctga tgtaccgtac aacgacaagg atgctacaga tttgatagct
      661 gctcgcgcac attccgtgag ggctaggcac cgtttttacc atgtacctgc cagcaacacg
      721 gcggtgggtc atctgattct ggagaatgtc tgatatgagc aaaaccatca aagttgcact
      781 ggctggcgcc ggtgctttcg gcatcaaaca cctggacggc atcaagaaca tcgatggtgt
      841 ggaagtggtg tccctgatca gccgcgacct ggagaagacc aaagaagtcg ctgacaaata
      901 tggcattggc catgtcacca ccgatctggc tgacagcctg gccctgccgg aggtggacgc
      961 ggtcattctg tgcactccca cgcagatgca cgctgagcaa acgattgcct gtctgaaagc
     1021 gggcaagcat gtgcaggtgg agattcccct ggcggattct cttaagggcg ctgaagccgt
     1081 ggtggctatg cagaagcaga ccgggctggt ggccatgtgc ggccacaccc gccgcttcaa
     1141 ccccagtcac cagtatgtga accagcagat caccgcgggc aagttcaaca tccagcagat
     1201 ggatgtgcag acctacttct tccgccgcac caacaccaat gcgctgggcc aggcccgcag
     1261 ctggaccgac cacctgctgt ggcaccacgc tgcccacacc gtggacctgt ttgcctacca
     1321 ggccaacagc cccatcgtgc aggccaacgc catccagggc cccatccatc cggtgctggg
     1381 catcgccatg gacatgagca tccagctgcg cgcggccaat ggcgccatct gcacgctctc
     1441 actaagtttc aacaacgatg gccccctggg cacctacttc cgctacatcg gcgacacggc
     1501 gacctacctg gcgcgttacg acgacctgta caccggcaag gaagagaaga tcgatgtgag
     1561 ccaggtggcc gtcagcatga acggcatcga actgcaggac cgcgagtttt ttgccgccat
     1621 ccgcgaaggc cgcgaaccca actccagcgt gggcaaggtt tttgcctgct accaggtgct
     1681 gcaccagttg gagcagcaac tggccgcaca gcggtaaggg gcggaccctg gccccatcgg
     1741 ggtcgtatgt gagccactgt cttcccaagg caaaataggg catgctggca atgttgtcgg
     1801 cagccgtatg ggggtggtct cgaatgaacg tgagccggat cggttggatc ggttttgttg
     1861 ccggtttggt tggcagtgtc agtgcgcagg cgaatcccgc ctctgccttg accgaacaag
     1921 agtttttggg cgaaatgccc gtagtgatct cggtctcacg gttggcacag cggctggatg
     1981 aaacacccgg tgccgtcacc attctggatc gcgacttcat ccgcatgtcc ggcgcgcgtg
     2041 atgtggccga tcttctgcgg gtggttccag gctttcaaac gaccaattct ttcgagacag
     2101 acgccccgat cgtgacctac cacggccgcg tggacgactg ggcaaaccgt gtgcaagtca
     2161 tggtggatgg tcgctctgtg tattcggggc tgttgcaggg gtctgcaggg ctggggctgg
     2221 aaacgctggc gctggcggac atcgagcgca tcgaggtgtt gcgcggctcc aactccgccg
     2281 cctacggtgc gcgtgccttt ctgggcgtga ttaatatcgt ttcgcgcgac ccccggcaga
     2341 cctttggcac gcaggcccag gcgacagtgg ggcagaacgg cgttgcggac ctcggcggaa
     2401 gcgtgggctg gggcgaagac tctgcggtgt tccgggtttc ggtcgattca cgcggggatg
     2461 atgggctgcg caagacaggg agtgctgctg gcagttccgg gaaaagccgc gtgaaccgcg
     2521 ccaacctggc cggtgccttg tcgctggggt cggccacgga gctgagcctg cacgccggcc
     2581 tgctcgatat tgcggcctac cggggcacgc tgatcggaga tgtcggaaat ccgcagcgca
     2641 tgcgcttcat gggctcgggc tatgttcagg gggaatgggc gcgcgcgttg agtgccgatg
     2701 aagatctgaa agtggaactt tcctacacgc gcattgagaa caaggacagt tacatctacc
     2761 agtcagatgg cccagccccg ggaacctacc ggggcatccg catctccctt gatgcggtgg
     2821 agcagaacgc agccgccagc ctgcaataca ttcgccgtta ttccaatgat gttcgcatgg
     2881 tgctgggtac cgagttgcgc agtgagcgca tcgattcacg ttccgagttc gaccgtgctg
     2941 gcaatgttga aacagacttc acccgcatct tcggcaatct ggagtggcgc atccatcccc
     3001 aatggctgct taacgccggg gccatgctgg aagacagcaa tgcggtggat gcgacgctgg
     3061 ctccccgatg gatgctgaac tggacgccgc tgcgaggcca caccttccgg gctggcagtt
     3121 ccacggcatt tcgtactccc agcgcttatg aaaacttcgc ggattctgct ttctacgata
     3181 tcaacggcca gaaccggctg tctgccgtcg tcggcagcgg acaactgcgc ccggagcgca
     3241 ttgtggcgcg ggaactcggc tatctgtttt acctggccgg gcgcacactg accggcgacg
     3301 tgcgcctgtt cgatgagcat atttcggatg gaattgccac tacggcgtct actccgaccg
     3361 cctttgtgaa ctccgaggac taccgcatta ccgggggtga gctggacctg gtctggcggc
     3421 cccatccggc cgtacgtgtc gggctgagtc atgcggcggc cgaagtccag atcaaccagt
     3481 cgcccaatct ctatcccaca gcgttcacga cgcaatacgc agttcccaag acgtcagatg
     3541 cactctcgct gatgttgaat ttgcctggcg ggtacagcgc ttcgctgatt tattcccagg
     3601 ccgagcgtct ggcgttgatg tcggcagtgg gcaagaccat caatatcgag cgcacggacg
     3661 tgcgcttggc aaagaacttc cgattgggta agtcgaaggc agagttggcg ttgacggtgc
     3721 agaacctgaa caacccctac cccgatgggg accgcaagtt ctatttcgat tcgcgggcca
     3781 tggtgaccct tcgcgtggaa aactgacgtt tgcctgccgt gcaccatttt gtccgcttcc
     3841 tgctgctggt gtcgctgact ttgctgtcgg gcggtgcgcg tgccatcgat gtggtggtgg
     3901 tgctcagtga gcgtggtccg ggctatctgg aagccacgga ggtgatggtg gcagagcttg
     3961 ggcgtgcggg tctgacggta tcggacaccg atgtgcgcag tgcaaacagt cttgcaaccg
     4021 cgctggctca gcaaacgccc agggtgattg tcacactcgg ctatgagtcg ctacgccagg
     4081 ttctggaggc ccgtccgcgc gtgccggtgc tggcagcgct gattccgcgc tcgggttttg
     4141 agcggctgtt gcgtgaccat gcctcgaccc gacccgccgt ggccgcgctg tacctggatc
     4201 agcccatcgg ccgccagatg gatgtgattc ggctggcgtt tggcgcaggg catcgggttg
     4261 ccgtattgct gggcccggag tcgtcatggc agaggagcct gctgacggcg tcggccggtg
     4321 cgcagggatt cagccttcag ttggccgagg tgggtgcttc gcaaacggta gcccatgcgc
     4381 tggcagatgt gctggatggt gcggatgttt tgctcgcctt gccagataca caggtctaca
     4441 acgcgactac cgtgggcaac gtgctcatga gcagctacag ggctcgcgta ccagtgctgg
     4501 cgttttcgcc ggcttatgtg cgtgccggag ccaccctgtc cttgcactcc acgccagtcc
     4561 aggtggccct gatggtgcgt cagcatcttc aggccggggc tgtgctgacc aatcaatacc
     4621 cggcggactt tgcgattacg gtgaacgaac gtgtcgcccg ctcccttgat ctcgacctca
     4681 atgccgccat gctcactgag caactgaagc ggttggcaag aaaaccatga gtggcggatc
     4741 catcaagtca cgcatgctgg cggttgccat actgccagtc acgctggtgg ttatcggcgt
     4801 cgtgctggtg ttctggctgg gtcgctccca ggacatggat gaagcacacg cccagcgcag
     4861 ccgattgctg gcgcggcagg tcgcactcgc cagcgaactg ggggtttttt ccggggatgt
     4921 cgccacactg caggggattg ttaacggggt actgcgcgaa cctgacgtca tcagtgctgc
     4981 ggtgtttgac actgccggcg tggccatggt gtcggccgga gaggtgcgca atgtgaccat
     5041 ggcccagatg ctcgacccgt tcacgctgca acagcggcag cgcttgcaga ttgacctgct
     5101 gaaggagccg gtggtggcgg ccgcattacc gatggaccat tggtttgcgc cggccgccgc
     5161 gcagcgcacc tccaaagtgc tgggctatgt gattctggag gtttcgcgtg cgagcctggt
     5221 gcagcaggag cttcgcatcc tgtggctggc gttgctggtg ggctcggtgg gcattctggg
     5281 gggcgggttg ctcgccttct atatggggcg
//
LOCUS       DAOSJM010000151         5305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13891_length_5305_cov_4.801333, whole genome shotgun sequence.
ACCESSION   DAOSJM010000151 DAOSJM010000000
VERSION     DAOSJM010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13891_length_5305_cov_4.801333"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /gene="metG"
                     /locus_tag="PKX82_06635"
     CDS             <1..980
                     /gene="metG"
                     /locus_tag="PKX82_06635"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HOF51965.1"
                     /translation="YLAAKLSARNEDIDFNADDFMARVNSDLIGKYVNIASRAAGFLT
                     KRFEGKLTSHFGATGSALLAEVRGASDALAQMYEAREYGKATREIMVLADKVNAYVDQ
                     NKPWDLAKDPANNEALHQVCSVLINTFAALTRYLAPVLPQLAQAVQGFVGSDMRHWNV
                     AGDVQAIAPYQHLMQRVTPEQLDALFEPPAPVESAQAATESVATIPGGEEIAPLIGID
                     DFTKIDLRIAKIVNCEAVEGSTKLLRLTLDAGEGRMRNVFSGIASMYKPEDLVGQLTV
                     LVANLAPRKMKFGVSEGMVMAASHADDKANPGIYILNPWPGATPGMRIH"
     gene            977..1765
                     /locus_tag="PKX82_06640"
     CDS             977..1765
                     /locus_tag="PKX82_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HOF51966.1"
                     /translation="MKTATLPAAEPPRPRWWADIHRNAEANLRLGCTLAAIAGATNAG
                     GFLAVGEYTSHMSGILSSIADNLVLGQLQLALASVALIVAFLSGSMTTAWIVNWAQRR
                     QMRSAFARPLFVEAFLLLVFGLFGAAISAFGVHFVPLTVLLLCYIMGLQNAVITKVSH
                     SEIRTTHVTGLLTDLGIEMGKMFYFNRTNLPNRVVANRRKVRIHGLLVASFFGGALLG
                     AFGFKFVGYISTVPLALVLLGLSWGPILDDMRHGRFASGPMGTP"
     gene            1762..3426
                     /locus_tag="PKX82_06645"
     CDS             1762..3426
                     /locus_tag="PKX82_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOF51967.1"
                     /translation="MITFRPRLLDTLHTYDRAHLVKDVGAGVTVGLVALPLAMAFAIA
                     SGLEPKAGIFTAIIAGFLISALGGSRVQIGGPAGAFIVIVYGIVHRYGVANLIIATAM
                     SGVMLFAMGLFKLGTLIRFIPVAVIIGFTNGIAVLVALSQIKDFLGLQTQGPVPADFF
                     GLVHTLWQALPTWNFLSAWVAMVCLVGLVCWQWVLPRVATRWPVFAPLQRLPGSVVML
                     VTSTAWVAFIGWDIETIGSRFGGIPSDLPGWQWPEFSWATVRFLFIPATTLALLGAIE
                     SLLCARVADGMIDDRHDSNQELMAQGVANLVTPFFGGMPATGTIARTMTNIKNGATSP
                     IAGMVHAATLLAIVLLAAPLAAHIPLSAMAAILMFVAWNMGEWREFAHLRQFRMPYRI
                     TLLSVFFLTVVFDLTVAVEVGLVAACITFIYRISSLSRSEQVSAQDYPVLAGMDKQVI
                     AHRMYGALFFGAVKLIESMEDHLPARVLVLDFKNVIYIDSSGADTVRDLMRTCHKQGV
                     RLIACGLDFQPKDILMRAGVLDVLGPDNLCPRLPEALELAVQDARA"
     gene            3478..5139
                     /gene="nadB"
                     /locus_tag="PKX82_06650"
     CDS             3478..5139
                     /gene="nadB"
                     /locus_tag="PKX82_06650"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOF51968.1"
                     /translation="MTDPISTPQTPAPLAFDVLIVGSGLAGLSAALLLPSDRRVAILT
                     KRAVREGSSGWAQGGIAAVWDKDDSFAAHVDDTLVAGAGLCDLQATQFVVENAPASIA
                     WLQKLGVPFSKEDGQLHLTREGGHSARRIVHVTDATGAAVQQTLIEKVAQSPNITLFE
                     NHTLVDLITSNKLAMKTGATHAESTGATGQKDTKNRCVGLYALDEDTDEVLTFQAPHT
                     ILATGGAGKVYLYTTNPDTATGDGIAAAWRAGCRVQNMEFIQFHPTCLYHPQAKSFLI
                     SEAVRGEGGRLLLPDGTRFMPAHDPRAELAPRDVVARAIDFEMKKGGFDCVYLDISHQ
                     PLAFIQEHFPNIYARCLELGIDMSKQPIPVVPAAHYTCGGVLTDLSARTDVAGLYAVG
                     ETACTGLHGANRLASNSLVECMVFARAATQAITGDRTTPADLPMPPAWDDSRVTDSDE
                     SVVISHNWDELRRFMWDYVGIVRTNKRLERAAHRIALLQSETQEYYAHFHVTRDLLEL
                     RNLVQVADLIVRSAQARHESRGLHFSRDYTSQLDKAVPTTLTPTH"
     gene            5151..>5305
                     /locus_tag="PKX82_06655"
     CDS             5151..>5305
                     /locus_tag="PKX82_06655"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOF51969.1"
                     /translation="MFRTLLKSKIHRVAVTQCELHYEGSCAIDEDLLAAANICENEQI
                     HIWNINNG"
BASE COUNT          958 a         1748 c         1591 g         1008 t
ORIGIN      
        1 actacctggc agccaagctg agtgcccgca acgaagatat cgacttcaac gccgacgact
       61 tcatggcccg cgtcaacagc gatctgattg gcaagtacgt gaacattgct tcgcgtgccg
      121 caggcttttt gaccaagcgc tttgaaggca agctgaccag ccactttggc gctaccggtt
      181 cggccctgct ggccgaggtg cgcggcgcca gtgatgcgct ggcccagatg tacgaagcgc
      241 gcgaatacgg aaaagccacg cgcgaaatca tggtgctggc cgacaaggtc aacgcctatg
      301 tggaccagaa caaaccctgg gacctggcca aagaccccgc caacaacgaa gcactgcacc
      361 aggtgtgttc ggtactgatc aacacctttg ccgctctgac ccgttatctt gcgccggtgt
      421 tgccccagtt ggcccaggcg gtgcaaggct ttgtcggtag cgacatgcgg cactggaatg
      481 tggctggtga cgtgcaggcc attgccccct atcagcacct gatgcagcga gtaacccccg
      541 agcagcttga tgcactgttc gagcctccag cccccgtgga atctgcgcag gcagctactg
      601 aatctgtagc aaccatccct ggtggcgaag aaatcgcccc gctgattggc atcgacgact
      661 tcaccaagat tgacctgcgc atcgccaaaa tcgtgaactg cgaagcggtg gaaggttcca
      721 ccaagctgct gcgcctgacg ctggatgccg gcgaaggccg catgcgcaat gtcttctccg
      781 gcattgccag catgtacaag cccgaagacc tggtgggcca gctcaccgtg ctggtggcca
      841 acctggcacc gcgcaagatg aaatttggcg tctccgaagg catggtgatg gccgccagtc
      901 acgccgatga caaggccaat ccgggcatct acatcctgaa cccctggccc ggcgcaacgc
      961 ccggtatgcg catccattga aaaccgccac gcttccagcc gctgagcctc cccgcccacg
     1021 ctggtgggcg gacattcacc gcaatgcaga ggccaacctg cggctgggtt gcacgctggc
     1081 ggccattgcg ggagccacca atgccggcgg ctttctggca gtgggggagt acacctcgca
     1141 catgtccggc atcctgtcgt cgattgccga caacctggtg cttggccagt tgcagctggc
     1201 actggccagc gtggccctga tcgttgcatt tctcagtggc tccatgacca ccgcgtggat
     1261 cgtgaactgg gcccagcgcc ggcagatgcg cagcgcgttt gcccgacccc tgtttgtcga
     1321 agcgtttctg ctgctggtgt ttggactgtt tggcgcagcc atcagcgctt ttggggtgca
     1381 ctttgttccc ttgaccgtgc tcttgctgtg ctacatcatg ggcctgcaaa acgcagtgat
     1441 caccaaggtc tcgcattcgg agatccgcac cacacacgtg accggattgc tgactgatct
     1501 ggggatcgaa atggggaaga tgttctattt caaccgcacc aacctgccca accgcgtcgt
     1561 agccaaccgg cgcaaagtgc gtatccacgg cctgctggtg gccagctttt ttggtggtgc
     1621 cctgctgggc gcattcggat tcaagttcgt gggctacatc tcgaccgtgc cgctggcgct
     1681 ggtgctgctg ggactgagct ggggccccat cctcgacgac atgcggcatg gccggtttgc
     1741 ctcggggccg atgggaaccc catgatcacg tttcgaccac gcctgctgga caccctgcac
     1801 acctacgacc gtgcgcacct ggtcaaggac gtgggcgccg gggtcaccgt aggactggtg
     1861 gcattgcccc tggccatggc ctttgccatc gccagcggac tggagcccaa agcggggatc
     1921 ttcaccgcca tcatcgcggg gttcctgatt tcggcgctgg gtggcagccg ggtgcagatt
     1981 ggcgggcccg ctggcgcatt cattgtcatc gtgtacggta tcgtgcaccg ctacggggtg
     2041 gccaacctca tcatcgccac cgccatgtcc ggcgtcatgc tgtttgccat gggcctgttc
     2101 aagctgggca cgctgatccg tttcattccg gtggcggtga tcatcggctt taccaacggc
     2161 atcgccgtac tcgttgcgct gtcgcaaatc aaggactttc tgggcctgca gacccaaggc
     2221 ccagtgcctg ccgacttctt cgggctggtg cacaccctgt ggcaagccct gcccacgtgg
     2281 aacttcctgt ctgcctgggt ggccatggtg tgtctggtcg gcctggtgtg ctggcaatgg
     2341 gtgttgccgc gggtagccac ccgctggccg gtcttcgcgc cattgcagcg cctgccaggt
     2401 tcggtggtga tgctggtcac cagcaccgcc tgggtcgcct ttatcggctg ggacatcgaa
     2461 accatcggca gccgctttgg cggcatcccg tccgacctgc cgggctggca gtggccagaa
     2521 ttttcctggg ccaccgtgcg ctttctgttc atcccggcca ccacgctggc gctgctgggt
     2581 gcgatcgaat cgctgctgtg cgcccgtgtg gccgacggca tgatcgatga ccgccacgat
     2641 tccaaccagg aactgatggc acagggtgtc gccaatctgg tcacgccgtt cttcggcggc
     2701 atgcccgcca ccggcacgat tgcccgcacc atgaccaaca tcaaaaacgg tgccaccagc
     2761 ccgatagcag gtatggtgca tgcggccact ttgctggcca tcgtgctgct ggcggctcct
     2821 ctggcagccc acattccttt gagtgccatg gccgccatcc tgatgttcgt ggcctggaac
     2881 atgggcgagt ggcgcgaatt tgcccacctg cgccagttcc gcatgcccta ccgcatcacg
     2941 ctgctgtcgg tgtttttcct gacggttgtc ttcgacctga cggtcgcggt ggaggtcggt
     3001 ctggtggccg cgtgcattac cttcatctat cggatttcca gcctgtcgcg ctccgagcag
     3061 gtcagcgcgc aggactaccc ggtgctggcc ggcatggaca agcaggtgat cgcacaccgg
     3121 atgtacggcg cgctcttctt tggtgcggtc aaactcattg aatccatgga ggaccacctg
     3181 cccgcgcggg tgctggtgct ggacttcaaa aatgtgatct acatcgattc ttcgggcgcc
     3241 gacacggtgc gcgacctgat gcgcacctgc cacaagcagg gcgtgcgcct gattgcctgt
     3301 ggcctggatt tccagcccaa agacattctg atgcgcgcgg gtgtgctgga tgtactaggg
     3361 ccagacaacc tgtgcccccg tctgcctgag gcactggaac tggccgtgca ggacgcacgc
     3421 gcctgacccg cccgcatcca ctttcgcggg tgtatcccgc cttcacgaat tgcccgtatg
     3481 accgatccca tttctacccc ccagacacct gctccactgg catttgacgt actgatcgtc
     3541 ggaagcggtc tggcgggcct gagcgccgct ttgctattgc cgagtgatcg cagggtcgcc
     3601 atcctcacca agcgcgcggt gcgcgagggc tccagcggct gggcgcaagg cggcattgcc
     3661 gccgtatggg acaaggacga cagctttgcc gcccacgtgg acgacacgct ggtggccggt
     3721 gccggcctgt gcgatctgca ggccacccag ttcgtggtgg aaaacgcacc ggcctccatc
     3781 gcctggctgc aaaaactcgg cgtgccattc tcgaaggagg atggccagtt gcacctgacg
     3841 cgcgaaggcg gccacagtgc acggcgcatc gtgcacgtga ccgacgccac cggcgcggcc
     3901 gtgcagcaga ccctgatcga gaaagtggca caaagcccca acatcacgct gttcgaaaac
     3961 cacaccctgg tggacctgat taccagcaac aagcttgcta tgaaaacagg agctactcac
     4021 gcagaatcca cgggggctac aggccaaaaa gacaccaaaa atcgctgtgt gggcctgtac
     4081 gcgctggacg aagacaccga cgaggtgttg accttccagg cgccccacac catcctggcc
     4141 accggcggcg cgggcaaggt ctacctctac accaccaacc ccgacaccgc caccggcgat
     4201 ggcattgccg cagcctggcg tgcgggctgc cgggtgcaga acatggagtt catccagttc
     4261 caccccacct gcctgtacca cccgcaggcc aagtccttcc tgatcagcga agcggtgcgc
     4321 ggcgaaggtg gccggctgct gctgcccgat ggcacccgtt tcatgccggc ccacgacccg
     4381 cgcgccgagc tggcaccgcg cgatgtggtg gcgcgtgcca tcgacttcga gatgaagaag
     4441 ggcggctttg actgcgtgta cctcgacatc tcgcaccagc cactggcctt catccaggag
     4501 cactttccca acatctacgc gcgctgcctg gagctgggta tcgacatgtc gaagcagccc
     4561 atccccgtgg tgcccgcggc ccactacacc tgcggcgggg tgctgaccga tctgtcggcc
     4621 cgcaccgatg tggccgggct gtacgccgtg ggcgaaaccg cctgcaccgg gctgcatggc
     4681 gccaaccgcc tggcatccaa ctcgctggtg gagtgcatgg tgtttgcccg ggccgccacg
     4741 caggccatca ccggtgaccg caccacaccc gccgacctgc ccatgccgcc agcctgggat
     4801 gacagccgcg tcaccgactc ggacgagtcg gtggtgatct cccacaactg ggacgaactg
     4861 cgccgcttca tgtgggacta cgtcggtatc gtgcgcacca acaagcgcct ggaacgcgca
     4921 gcccaccgca ttgcgctgct gcagagcgaa acgcaggagt actacgccca cttccatgtc
     4981 acgcgcgacc tgctggagct gcgcaacctg gtgcaggtgg ccgacctgat cgtgcgcagt
     5041 gcgcaagcgc ggcacgagag ccgcggcttg cacttcagcc gcgattacac ttcgcagctc
     5101 gacaaggcgg tccccaccac cttgacaccg acccattgat tccaacaacc atgttccgaa
     5161 ccctgctgaa atccaaaatc caccgcgtgg ccgtgaccca gtgcgaactg cactacgaag
     5221 gctcctgcgc catcgacgag gacctgctag ccgccgccaa catctgcgag aacgaacaga
     5281 tccacatctg gaatatcaac aatgg
//
LOCUS       DAOSJM010000152         5283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13963_length_5283_cov_4.282708, whole genome shotgun sequence.
ACCESSION   DAOSJM010000152 DAOSJM010000000
VERSION     DAOSJM010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5283
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13963_length_5283_cov_4.282708"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1321
                     /locus_tag="PKX82_06660"
     CDS             <1..1321
                     /locus_tag="PKX82_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF51970.1"
                     /translation="VDPVVRVSRIVARIGNGDFSPIPPVSPSDPLHKLHLALDGMAQR
                     LSWSRDELEQRVTQVTQELLARKEEAELATRAKSRFLAAASHDLRQPTHALGLFVTRL
                     GQLPLDAQARAVVDNLESSVLAMQDLLDGLLDLSRLDAGNVTTAVAPLAVDSVLESVR
                     DVLEPLAVQRGLQLRVRLSGLWVVSDLFLLKRMVLNLGQNAVRYTLQGGLLLTARPCD
                     QGRAVRIEVWDTGIGIAAEHHDDVFKEFFQVGNRSRDRQQGLGLGLSIVKRSAELLGH
                     QVDVRSVAGRGSRFSIVVPRTAPVDPKMLQEPVQATVRADEQGMRVLLIEDDPLALAS
                     VVGLLTSWGCEVLATRSAEEACALIANGAQPSVLLSDYRLGGDATGIDAIVAVRAGLG
                     RALPACLISGDTDADLMQRAKALGLTLLHKPVRPAKLRSLLRSIAAR"
     gene            complement(1298..1939)
                     /locus_tag="PKX82_06665"
     CDS             complement(1298..1939)
                     /locus_tag="PKX82_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF51971.1"
                     /translation="MSSVKILVVDDHALVREGLSQVLKALADEVTVLQASTCLQAFEL
                     SERHCDLDLVLLDYHLPDMTGLEALPIFRRRFPELPVLVLSGSANVQILHQALKAGAA
                     GYIQKSGVNDELLSAVRKILDGEVFVSAPAPEIARVESEYSRLTPRQELVLRGILDGL
                     SNKDIGDRIGISEETVKTHVAAILRYFHAQNRTQAVTTAMQQGYRPSSRRNTA"
     gene            complement(1982..3298)
                     /gene="deoA"
                     /locus_tag="PKX82_06670"
     CDS             complement(1982..3298)
                     /gene="deoA"
                     /locus_tag="PKX82_06670"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOF51972.1"
                     /translation="MLAQEIIRRKRDGHRLTTAEIEDFVKGLTTGTWSEGQAAAMAMA
                     MFLVGMSSEEKVALTLAMTRSGTVMDWSSTELPGPILDKHSTGGVGDKVSLMLAPIVA
                     ACGGFVPMISGRGLGHTGGTLDKLDSIPGYQTAPDTQRLRQTLRASGCAIIGQTSELA
                     PADRRLYGIRDVTATVESVALITASILSKKLAAGLQALVMDVKVGNGAFADSPAMAEE
                     LARSLVTVANGAGLPTRAWITDMNQVLGDTCGNALEVQEAVAFLQGSRREPRLLEVTR
                     TLSAELLQLGGLAPDMAQALQQVDRTLESGHALEHFARMVSALGGPADFCDRPARYLA
                     AAPAKCVVKAPADGWVTGMATREIGLQVVQLGGGRRMASDTVDARVGMTGFAQIGQRV
                     QAGDPLANVHAASIEAARACADALQTLVHIGNQEPTCTPILIQSIS"
     gene            complement(3310..4101)
                     /gene="deoC"
                     /locus_tag="PKX82_06675"
     CDS             complement(3310..4101)
                     /gene="deoC"
                     /locus_tag="PKX82_06675"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOF51973.1"
                     /translation="MQASSIPWQQDLKATARVALACLDLTSLNDADTEADIARLCARA
                     QGPFGAVAAVCVWPRLAAFARAQLPASIAVAAVANFPHGSADVQQAVRDTGTIVQSGA
                     QEVDVVLPYRQLMAGDDPTVAALLMAVRKACEGLRLKVILETGKLQDEALIKRACRLS
                     LDAGADFLKTSTGKTPVSATPQAARTLMAAIAADPRCSARVGFKPSGGIRAVADAATY
                     IALTEEYLGTAALKPLRFRIGASSILSDIEAVLDDRTAPAAAGTY"
     gene            complement(4119..4937)
                     /locus_tag="PKX82_06680"
     CDS             complement(4119..4937)
                     /locus_tag="PKX82_06680"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOF51974.1"
                     /translation="MTDASLLPALQTIRQRALDVAPRIAVVLGSGWGELTQHIAHPTR
                     IPYSELEGFPAATVTGHSGELWLGTLGHHQVAVMSGRKHGYETGAVDGMKTPLRVLQA
                     LGCEVLVQTNAAGSLRASMPPQSLMLLTDHINVPQRTPLLDEAGNARFVSMVDAYDPA
                     LRQQALTMAARMDIPLHQGVYAWAFGPQFETPAEIRMLQMLGADAVGMSTVPETILAR
                     HAGLRVLALSFITNMGAGLSTEALSHAHTLSQAQAGSVQASRMLAAIVGGLDLS"
     gene            complement(4934..>5283)
                     /locus_tag="PKX82_06685"
     CDS             complement(4934..>5283)
                     /locus_tag="PKX82_06685"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOF51975.1"
                     /translation="PYSCFPVGVAVLDDQGRIHAGCNVENAAYPEGVCAEASALSAMV
                     LAGSTRLRAVLVVGTGGAWITPCGGCRQKLREFGAPDLPVLTANATDMGPRYTLAELL
                     PQSFGPDHLGTTP"
BASE COUNT          878 a         1649 c         1770 g          986 t
ORIGIN      
        1 ggtggacccg gtggtgcggg tgtcacggat cgtggcccgc atcggcaatg gtgatttctc
       61 accgattcct ccggtgagcc ccagtgatcc gcttcacaaa ctgcatctgg cactggacgg
      121 catggcgcag cgcctctcgt ggagcaggga tgagctcgag cagcgcgtga cccaggtcac
      181 gcaggaattg ctggcccgaa aggaggaggc cgaacttgcc acgcgtgcca aatcccgctt
      241 tctggccgct gccagtcacg acctgcgcca gcccacccat gcgttgggac tgtttgtcac
      301 ccgcttggga cagctgccgt tggatgcgca agcacgggcg gtggtcgaca acctggagag
      361 ttctgtgctg gccatgcagg acttgctcga tggcctgctg gacctgtcgc gactggatgc
      421 tggcaatgtg accactgccg tagccccatt ggccgtggac tcggtgctgg agtcggtgcg
      481 tgacgtgctg gagccactgg cagtacagcg ggggttgcaa ctgcgcgtcc ggctcagcgg
      541 gctgtgggtc gtgagtgacc tgttcctgct gaaacgcatg gttctgaacc tggggcaaaa
      601 cgcggttcgg tatacccttc aaggggggct tttgctgacg gcccgcccct gtgatcaggg
      661 ccgcgcagta cgcattgaag tgtgggacac gggcattggc attgcggcag agcaccacga
      721 cgacgtcttc aaggaatttt ttcaggtggg aaatcgctcg cgcgaccgcc agcagggcct
      781 gggtctgggc ttgagcattg tcaagcgcag tgccgaattg ctggggcacc aggtcgatgt
      841 ccgatcggtg gcggggcgtg gcagccggtt ctccatcgtg gtgccccgca cagcacccgt
      901 ggatcccaag atgctccagg agcctgtaca ggcgacagtc agggctgacg aacagggtat
      961 gcgggtgctc ctgattgaag atgatccgct ggccctggcc tctgtggtgg gtcttctcac
     1021 gtcctgggga tgtgaggtac tggccacccg cagcgccgaa gaagcgtgtg cgctgattgc
     1081 caacggtgcg cagcccagtg tgcttctgag cgactatcgc ctgggtggcg acgctaccgg
     1141 gatcgatgcc attgtggcgg tgcgcgccgg gttggggcgg gcgttgccgg cctgcctgat
     1201 cagcggtgac accgatgccg acctgatgca gcgtgccaag gcccttgggc tcaccttgtt
     1261 gcacaagccg gtgcggcctg cgaagctgcg cagcctgtta cgcagtattg cggcgcgatg
     1321 aggggcgata tccctgctgc atggccgttg taactgcttg cgtgcggttt tgcgcgtgga
     1381 aatagcgcag gatggccgcc acgtgggtct tgacggtttc ttccgaaatt ccgatcctgt
     1441 cgccgatgtc cttgttggat aaaccgtcca gaatgcccct gagtaccagc tcctggcgcg
     1501 gggtgagtct gctgtattca ctttctacgc gggcgatttc cggtgctggt gcggaaacaa
     1561 acacctcgcc gtccagaatc ttgcggacag cactcaacaa ctcgtcattg acacccgatt
     1621 tctggatgta gcctgccgca cccgccttca gtgcctgatg caggatctgt acattggcag
     1681 agccagaaag caccagtacc ggcagttcgg gaaaccggcg tcgaaaaatt ggtaacgctt
     1741 ccagtccggt catgtcgggc agatggtagt cgagcaacac caggtccaga tcgcagtggc
     1801 gttccgacag ctcgaatgcc tgaaggcagg tgctggcttg caacacggtc acttcatccg
     1861 cgagtgcctt gagcacctgg ctgagccctt cgcgcacgag ggcatggtca tccaccacca
     1921 atatttttac gctgctcatg gggttgaagt atggcttaga taggcgtcaa tgccggtcca
     1981 atcaggaaat ggactggatc aggatgggcg tgcaggtcgg ttcctggtta ccgatgtgga
     2041 ccagcgtctg caatgcatca gcgcacgccc gggcggcctc gatggatgcg gcgtgcacgt
     2101 tggcgagcgg gtcacctgcc tggacacgct ggccgatctg ggcaaagccc gtcatgccca
     2161 cccgcgcatc cacggtgtcg gacgccatgc gccgaccgcc accgagctgc accacctgca
     2221 agccaatctc tcgggtggcc atgccggtca cccagccgtc tgcgggtgct ttcaccacgc
     2281 acttcgccgg agctgcggcc aggtagcggg ccgggcgatc acaaaagtcc gcagggccac
     2341 ccagtgcgct gaccatgcgc gcaaagtgct ccagcgcatg gccactctct agcgtgcgat
     2401 ctacctgctg caaagcctgc gccatgtcgg gggcgaggcc acccaactgc agcagttctg
     2461 cgctgagggt gcgggtgact tccagcagac ggggctcgcg acgggagccc tgcaaaaagg
     2521 ccacggcttc ctgcacttcg agcgcattgc cgcaggtgtc acccagcacc tggttcatgt
     2581 cggtaatcca ggcgcgcgtg ggcaggccgg cgccgttggc gacagtcacc agtgaacgcg
     2641 ccagctcttc tgccatggcc ggtgaatctg caaacgcgcc attgcccact ttgacgtcca
     2701 tcaccagcgc ttgcagccct gccgccagct tcttggagag gatgctggcg gtgatcagcg
     2761 cgaccgattc cacggtggct gtgacatcgc ggatgccata gaggcgccgg tctgccggag
     2821 ccagctcaga ggtctggccg atgatggcgc agcccgatgc ccggagcgtc tggcgcaggc
     2881 gctgcgtatc gggcgccgtc tggtagccgg ggatgctgtc gagtttgtcc agcgtgccgc
     2941 ccgtgtggcc caggccgcgg cccgagatca tgggaacaaa gccgccacag gcggccacga
     3001 tcggcgccag catcaggctg accttgtcgc ctacgccgcc ggtggagtgc ttgtccagaa
     3061 tcgggcctgg cagctcggtg ctgctccagt ccatcaccgt gcccgaacgt gtcatggcca
     3121 gggtcagcgc gactttctcc tcgctggaca tgcccaccag aaacatggcc atcgccatgg
     3181 ccgcggcctg gccttcgctc caggtgccgg tggtcagccc tttgacgaag tcctcgatct
     3241 ccgcggttgt caggcgatgt ccatcgcgtt tgcgccgaat gatttcctgg gccagcatgg
     3301 cgtagctcct cagtaggttc cggcggcagc cggcgcagtg cggtcgtcca gcaccgcttc
     3361 aatatcggac aggatgctgc tggcgccaat gcgaaagcgc aggggcttga gcgcggccgt
     3421 gccgaggtat tcctcggtca gtgcgatgta ggtggcggcg tcggccacgg cgcggatgcc
     3481 gcccgagggt ttgaagccca cgcgggcgct gcagcgtgga tcagcggcaa tggctgccat
     3541 cagggtgcgc gccgcctggg gcgtggcact gaccggcgtt ttgcctgtgc tggttttgag
     3601 aaagtcggct cccgcgtcca ggctcaggcg gcaggcccgc ttaatcaggg cttcgtcctg
     3661 cagcttgccg gtctccagaa tcactttcag gcgcaatccc tcgcaggcct tgcgcaccgc
     3721 catcagcagg gctgcaaccg tggggtcatc acctgccatg agctgccggt aaggcagcac
     3781 cacatccact tcctgcgcgc cactttggac aatggtgccg gtgtcgcgca ctgcctgctg
     3841 tacatcggcg ctgccatgcg gaaagttggc cacggcggcc accgcaatgc tggcgggcaa
     3901 ctgggcgcgt gcaaatgccg ccagacgggg ccagacgcac actgcggcca cagcgccaaa
     3961 ggggccttgg gcgcgcgcgc agagcctggc gatatcggcc tcggtgtccg catcgttcag
     4021 gctggtgagg tccaggcagg ccagggcgac gcgcgctgtg gctttgaggt cctgctgcca
     4081 agggatagaa gatgcttgca tcggggatgt gtctcggttc aggaaaggtc caggccgcca
     4141 acaatggcgg cgagcattcg gctagcctgc acgctgccgg cttgcgcctg tgacaacgtg
     4201 tgcgcgtggg acagcgcttc cgtcgacagg cccgcgccca tgttggtgat gaaggacagc
     4261 gcgagtaccc gcaggcccgc gtggcgcgca aggatggttt caggcaccgt gctcatgccc
     4321 accgcgtcgg cccccagcat ctgcagcatg cggatttctg caggggtttc aaactgcggg
     4381 ccaaaggccc acgcatacac accctggtgc agagggatgt ccatgcgcgc agccatggtc
     4441 agcgcctgct ggcgcaacgc cgggtcgtag gcatcgacca tgctgacgaa tcgcgcattg
     4501 ccagcttcat ccagcagcgg cgtgcgctgg gggacattga tgtggtccgt caacagcatc
     4561 aggctttggg gcggcatgct tgcgcgcagg ctgccggccg cattggtttg caccagcact
     4621 tcgcagccca gcgcctgcag tacccgtagc ggcgttttca tgccgtccac ggcgccggtc
     4681 tcgtagccat gcttgcgccc gctcatcacc gcgacctggt ggtggccgag cgtgcccagc
     4741 cacagctcgc cgctgtgccc agttacggtg gctgccggaa agccttcgag ctcggaatag
     4801 ggaatccgcg ttgggtgcgc gatgtgctgg gtcagttcgc cccagccgga gcccaaaacg
     4861 acggcaatgc gcggcgccac atcgagggca cgctggcgga tggtttgcaa tgcaggcagg
     4921 agcgatgcgt ccgtcatggc gtggtgccca ggtggtccgg gccaaagctt tggggcagca
     4981 gttctgccag cgtgtagcgg gggcccatgt ccgtggcatt ggcggtcagc actggcaggt
     5041 cgggcgcacc gaattcgcgc agcttctggc ggcagccgcc gcacggtgtg atccatgcac
     5101 caccggtgcc caccaccagc acggcgcgca atcgggtgct gccggccagc accatcgcgc
     5161 tcagggcgct ggcttcggcg cacacgcctt ccgggtaagc cgcgttttcc acattgcagc
     5221 ctgcgtggat ccggccctgg tcgtccagca ccgcgacacc gaccgggaag caggagtagg
     5281 gtg
//
LOCUS       DAOSJM010000153         5273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_13997_length_5273_cov_5.096014, whole genome shotgun sequence.
ACCESSION   DAOSJM010000153 DAOSJM010000000
VERSION     DAOSJM010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13997_length_5273_cov_5.096014"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PKX82_06690"
     CDS             complement(<1..201)
                     /locus_tag="PKX82_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine methyltransferase"
                     /protein_id="HOF51976.1"
                     /translation="MKFSNQLTRCTFDGPLGSMTLAAHDHGLVGIWFDGQQHQPDPSV
                     WAVNPRHPMLASATRQLQEYFAG"
     gene            complement(252..1757)
                     /locus_tag="PKX82_06695"
     CDS             complement(252..1757)
                     /locus_tag="PKX82_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HOF51977.1"
                     /translation="MPHASLPDSDDARYLALSTRDSRFDGRFFVGVTSTGIYCRPVCK
                     VRLPKRENCRFYVLAAQAEQAGFRPCLRCRPELAPTTTTPIWSTQDASAILAQQAARL
                     LDSAEPWCEGTPNAGQLATRLGISERHVRRIFEAHWGVSPLQYLQTRRLLTAKQLLTD
                     TRLPIAEVAHLSGFASLRSFNAAFVSHYHLQPRSLRKDAASASPSAMTADRPAGLVLK
                     ASYRAPYDVAAMLGFFAARAVAGLEVVHLQEQWMARTLEVWHAGRIHAGWVHLQFVHG
                     RQEVVFRIDEALAGVLPLVMSRLHGLLDLDADPLPIQAQLGARFPGSEGLRVPGTLDG
                     FELAVRAILGQQITVAAARTLTTRLVARFGRPLVTPFAELTHLFPGASTLSQASGEDL
                     GALGIVRQRQAAILALAQAVAAGRIQLHASADVPATMDALQALPGIGAWTAHYIAMRA
                     LRWPDAFPSGDVALQKALGVRETKHPAAEAERASQAWRPWRSYAVLRAWSL"
     gene            1884..3266
                     /locus_tag="PKX82_06700"
     CDS             1884..3266
                     /locus_tag="PKX82_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOF51978.1"
                     /translation="MQISSSIFKAYDIRGVVPSTLTEDVALGLGRAFGTVALAQGETT
                     VAVGRDGRLSGPALSQALMRGLQEAGVAVIDVGMVTTPMLYFAANTLCNSGIQVTGSH
                     NPKDYNGFKMVMGGRAIFGDDIQALRRMMEAETWTLKAGGTVTARDVTAAYRERIAGD
                     IKLARPIKIVVDSGNGIAGASAPDILRAIGCEVQELHSTVDGNFPNHHPDPSKPENLR
                     DVIAALASGDAELGLAFDGDGDRLGIVTKDGTNIYPDRQMMLFAQDVLSRVPGGTIVY
                     DVKCSQRLGPAIAAAGGVPLMFKTGHSLIKAKMKEIDSPLGGEMSGHIFFKERWYGFD
                     DGTYAGCRLLEIVSRAQDASAVLNALPTSFSTPELNVPCAEGEPPVVVQALVAKAAFE
                     APAVVNTIDGLRVDWPDGFGLVRASNTTPVLVLRFEGQTQAALDRIQKAMVALLHSVK
                     PDAHFEKAAH"
     gene            3263..4288
                     /gene="waaC"
                     /locus_tag="PKX82_06705"
     CDS             3263..4288
                     /gene="waaC"
                     /locus_tag="PKX82_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HOF51979.1"
                     /translation="MSSTLRKVLIVKLSSLGDVVHAMPAVQDLRSALPGVQIDWVVEG
                     GFVPLAARCQGVGRVIACDLRRWRKSPFAKQTRQEWKQFKTELQSEAYDAVIDLQGLT
                     KSALVAWLARTTPTGQRISLAHATDGSSFEAPARWVADLAVTLDPHVHAVQRSRLLCA
                     KALDYEVPKKLQFGLKPGALAAVPPEFTANPFVPRKPVVALVHGTSRADKQWPVDAWI
                     ALGQRLNHSGFAVALAHGSAVEKATSETIAVGLDDAWVWPTMALDALTDTMARCAGVV
                     GVDSGLSHIAVALGLPHVQIYNFDTAWRTGPLENGRQVSVFAAPHPEVDAVWQAWESL
                     ATPVLCQ"
     gene            4285..>5273
                     /locus_tag="PKX82_06710"
     CDS             4285..>5273
                     /locus_tag="PKX82_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOF51980.1"
                     /translation="MMRWLYSLVLWVAQPLLRSKLRRRAVAEPGYGEAVEERFGNYTQ
                     ARADTGSAAGPCIWVHAVSLGEARAASALVSQLRSAFPGMRLLLTHGTATGRAQGKAL
                     LQSGDVQVWQPWDTPRAVARFLQHFQPRVGLLVETEVWPNMVAACAKRGIPLCLVNAR
                     LSEKSLRQAQRLSWLSRPAYAGLRVVWAQTEEDAARLRALGATVQGVMGNFKFDVTPD
                     ATQLQTGAAWREKLGRPVVMFASSREGEERLLLEILKQNRPLAPVDTAQAATENVANA
                     VQWLIVPRHPQRFDEVARLCIEAGFSVSRRSQWTDGPQHADIWIGDTLGEMAL"
BASE COUNT          938 a         1589 c         1807 g          939 t
ORIGIN      
        1 tccagcgaag tattcctgta gttgacgggt agcgctggct agcatggggt gacgtggatt
       61 cacagcccag acggacgggt ccggctggtg ctgttgtccg tcaaaccaga tgcccacaag
      121 tccgtggtca tgggccgcaa gcgtcatgga ccccagcgga ccgtcaaagg tgcagcgcgt
      181 gagttggttg gagaatttca tataaaaatg ccttgtagcc ctcgttgaat gtgcacaatc
      241 agctattgtt tttatagcga ccaggcacgc agcaccgcat agctgcgcca cggccgccag
      301 gcctgggagg cgcgctcggc ttctgcagca ggatgttttg tctcgcgtac acccagggcc
      361 ttttgcagcg ccacatcgcc cgagggaaac gcatcgggcc agcgcaaggc acgcatggcg
      421 atgtagtgcg cggtccaggc gccaatgcca gggagcgcct gcagtgcgtc catggtggcc
      481 ggtacatcgg cgctggcatg cagctggatg cgtcccgcgg ccaccgcttg ggccagggcg
      541 aggatggccg cctggcgctg gcgaacaatg cccagggcgc ccaggtcttc tccgctggcc
      601 tgtgacaggg tgctggcacc ggggaataga tgtgttagct cggcgaacgg ggtcaccagc
      661 gggcgcccga agcgggcgac cagtcgggtg gtcaatgtgc gtgctgcggc caccgtgatc
      721 tgctggccca ggatggcgcg taccgccagc tcgaaaccat cgagcgtgcc cgggacgcgc
      781 agcccctcag atccaggaaa gcgcgcgccc agttgcgcct ggatgggcag ggggtcggca
      841 tccaggtcca gcagaccatg caggcgggac atcaccaatg gcaatacgcc ggccagggct
      901 tcgtcgatgc gaaacaccac ctcctggcgc ccgtgcacaa actgcagatg cacccaaccc
      961 gcatggatgc gaccggcgtg ccagacctcc agtgtgcgcg ccatccattg ctcctgcagg
     1021 tgcaccacct ccagcccggc taccgcacgt gcggcaaaaa atccgagcat ggcggccacg
     1081 tcgtagggcg cgcggtagct ggccttgagc accaacccgg cgggtctatc tgccgtcatg
     1141 gcagagggtg aagcgctggc agcgtccttg cgcaggctgc ggggctgcag gtggtagtgc
     1201 gagacaaagg ctgcattgaa gctgcgcagg ctggcaaacc cactgaggtg ggcgacctcg
     1261 gcaatcggca ggcgggtgtc ggtcagtaac tgcttggcgg tcagcagccg gcgtgtctgc
     1321 aggtactgca gcggcgagac gccccaatgg gcttcaaaaa tccggcgcac atgccgctcg
     1381 ctgatgccca gccgggtggc cagttggcct gcgttgggcg tgccttcgca ccagggttcg
     1441 gcagagtcca gtaaacgcgc tgcctgctgc gccaggatcg cggaagcatc ctgggtggac
     1501 cagatcggtg tggtggtggt tggcgccaac tcgggccggc agcgcaggca gggccgaaag
     1561 ccagcctgtt cggcctgtgc ggccaacaca tagaagcggc agttctcgcg cttgggcagc
     1621 cgcaccttgc agacggggcg gcagtaaata cccgtggagg tgacgccaac gaagaaacgc
     1681 ccgtcaaacc ggctgtcccg cgtgctcagt gcgaggtaac gggcgtcatc cgaatcaggt
     1741 aggctggcgt ggggcatacg ggcatcatac gcagccaaac cgtgaaaaac tggcggtttc
     1801 cggacatgtg gccacaagcc cggcggccta caatgccagc gacgcgccac tccattcgtc
     1861 ggcgatccaa agacagggaa aacatgcaaa tttcatccag cattttcaag gcctatgaca
     1921 tccggggcgt cgtgccctcg acattgaccg aagacgtggc actgggactg ggccgtgcct
     1981 ttggcactgt ggcgctggcg cagggggaaa ccacggtggc tgtgggccgg gacgggcgtc
     2041 tgagcggccc cgccctgtcg caggccctga tgcgcggttt gcaggaagct ggcgtggccg
     2101 tgatcgatgt gggcatggtg actacgccca tgttgtactt tgcagcgaac acgctgtgta
     2161 acagcggcat ccaggtgacc ggcagccaca accccaagga ctacaacggc ttcaagatgg
     2221 tgatgggtgg gcgggccatt tttggcgacg acatccaggc cctgcgccgc atgatggaag
     2281 ccgaaacctg gacgctgaaa gccggcggca cggttaccgc gcgcgatgtg acggcggctt
     2341 accgcgagcg catcgcgggc gacatcaagc tggcccgccc gatcaagatc gtggtggact
     2401 cgggcaatgg cattgccggc gcatccgccc ccgatatcct gcgtgccatt ggctgcgagg
     2461 tgcaggaact tcacagcacc gtggatggca acttccccaa ccaccatcct gaccccagca
     2521 agcccgagaa cctgcgcgac gtgatcgcag cgctggcatc gggcgatgcc gagttggggc
     2581 tggctttcga tggcgacggt gaccggctgg gcatcgttac caaggatggc accaacatct
     2641 atccggaccg ccagatgatg ctgtttgcgc aggacgtgct ctcgcgcgtg cctggcggca
     2701 ccatcgtcta tgacgtcaag tgctcgcagc gcctgggccc ggccatcgcc gcggctggtg
     2761 gcgtgccact gatgttcaag acaggccact cgctgatcaa ggccaagatg aaggagatcg
     2821 actctccact gggcggcgag atgagcgggc acatcttctt caaggagcgc tggtacggtt
     2881 ttgacgacgg cacctacgcg ggttgccgcc tgctggaaat cgtcagccgc gcccaggatg
     2941 ccagtgcggt actcaacgcc ttgcccacca gcttctccac accggaactc aacgtaccgt
     3001 gtgcggaggg tgagccgccg gtcgtggtgc aggcactggt cgccaaggcg gcttttgaag
     3061 cgccggcggt cgtcaacacg atcgatggtt tgcgcgtgga ctggccggat ggtttcggtc
     3121 tggtgcgcgc atcgaacacc acgccagtgc tggtgctgcg ttttgaaggg cagacccagg
     3181 ctgcgctgga ccgcatccaa aaggccatgg tggcgttatt gcacagcgtg aagccggacg
     3241 cccattttga gaaggctgcg cattgagttc aacgctgcgc aaggtcctga tcgtcaagct
     3301 ctcgtccctg ggtgatgtgg tgcatgccat gcctgcggtg caggacctgc gttctgcgtt
     3361 gcccggcgtg cagattgact gggtagttga aggtggattt gtccctcttg ccgcgcgctg
     3421 ccagggggtg ggccgtgtca ttgcctgtga cttgcggcgc tggcgcaaga gtccctttgc
     3481 caagcagacc cggcaggagt ggaagcaatt caagacggaa ctgcaaagcg aagcgtacga
     3541 cgccgtcatc gacctgcagg gcctgaccaa atcggcactg gtggcatggc tggcgcgtac
     3601 cacgcccacc ggccagcgca tcagcctggc ccatgcgacc gatggctcca gctttgaggc
     3661 gccggcgcga tgggtggccg acctggccgt cacactggac ccccatgtgc acgccgtgca
     3721 gcgctcgcgc ctgctgtgcg ccaaggcgct ggactatgaa gtaccgaaaa agttgcagtt
     3781 cggtttgaag ccgggtgcat tggcggctgt accgcctgag ttcacagcca acccgtttgt
     3841 gccgcgcaag ccggtggtgg cgttggtgca cggcacctcg cgcgccgaca agcagtggcc
     3901 ggtggatgcg tggattgcgc tcgggcaacg tctcaaccac agcggatttg cggtggcact
     3961 ggcccatggc agtgctgttg aaaaggccac cagcgaaacc attgccgtgg ggctggacga
     4021 cgcctgggtg tggcccacca tggcactcga tgcgctgacc gacaccatgg cgcgctgcgc
     4081 gggcgtggtg ggggtggata gcggcctgag ccatatagcc gtggcgctgg gcctgcccca
     4141 tgtgcagatc tacaacttcg ataccgcctg gcgcaccggg ccgctggaga atgggcggca
     4201 ggtgagtgtg tttgccgcac cgcatccgga ggtcgatgcg gtctggcagg cctgggaaag
     4261 cctggccacc ccggtgctgt gccaatgatg cgctggctct attccctggt gctgtgggtt
     4321 gcacagcccc tgctgcggtc caagttgcgg cgcagggcag tggccgaacc gggctatggc
     4381 gaagcggtgg aagaacggtt tggcaactac acccaggcgc gggcggacac gggttctgcc
     4441 gcgggtccct gcatctgggt gcacgccgtc tctttgggcg aagcccgtgc ggcctctgcg
     4501 ctggtcagcc aattacgcag tgcattcccc ggcatgcgcc tgctgctcac ccacggcacc
     4561 gccacagggc gcgcgcaggg caaggccctg ttgcaatcgg gtgatgtgca ggtgtggcag
     4621 ccctgggata cgccccgtgc ggttgcgcgt tttctgcagc atttccagcc gcgcgtgggc
     4681 ttgctggtgg aaacggaagt ctggcccaac atggtggctg cgtgcgccaa gcggggcatt
     4741 cccctgtgcc tggtcaatgc gcgtctgagt gaaaagtcgc tgcggcaggc acagcgcctg
     4801 tcctggctgt cgcgccctgc ttacgcgggc ttgcgcgtgg tgtgggccca gaccgaggaa
     4861 gatgcggcgc gcctgcgtgc attgggagcc acggtgcagg gggtgatggg gaacttcaag
     4921 tttgacgtca caccggacgc tacccagttg cagaccgggg cggcctggcg tgaaaaactg
     4981 gggcggcccg tggtgatgtt cgccagctcc cgcgaggggg aggaacgtct tctgcttgag
     5041 attttgaagc aaaataggcc tctagccccc gtggatactg cgcaagcagc tactgaaaac
     5101 gtagcaaacg cagtgcagtg gctgattgtt ccccggcatc cgcagcgttt tgatgaagtg
     5161 gcccgcttgt gcattgaggc cgggttctct gtttccaggc gcagccaatg gaccgatggg
     5221 ccgcagcacg ccgacatctg gattggcgac acgctgggcg agatggcttt gta
//
LOCUS       DAOSJM010000154         5257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14041_length_5257_cov_4.105152, whole genome shotgun sequence.
ACCESSION   DAOSJM010000154 DAOSJM010000000
VERSION     DAOSJM010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14041_length_5257_cov_4.105152"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="PKX82_06715"
     CDS             complement(<1..820)
                     /locus_tag="PKX82_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HOF51981.1"
                     /translation="MAKTVADVMKMVKENEVKFVDFRFTDTRGKQQHTTVPVSHFDED
                     KFSSGHAFDGSSIAGWKGIEASDMLLMPDPNTANIDPFFEETTLIMNCDVVEPADGKS
                     YDRDPRSIAKRAEAYLKASGIGDAAFFGPEPEFFIFDGVRWSTEPNNTFYEIDEYEAP
                     WNTGSKLEGGNRGHRPTVKGGYFPVPPVDSTQDMRAEMSLILESLGIPVEVFHHEVAG
                     AGQNEIGTRFSTLVERADWTMLQKYVIHNVANAYGKTATFMPKPYHGDNGSGMHV"
     gene            complement(1043..1852)
                     /locus_tag="PKX82_06720"
     CDS             complement(1043..1852)
                     /locus_tag="PKX82_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOF51982.1"
                     /translation="MNFGLIIIGDEILSGKRTDKHLAKAIEVLGARGLQVAYADYVGD
                     APDRITATLRRAFASGDVVFSFGGIGATPDDHTRQCAARALGCELELHREAEALIRER
                     MQDVAREQGVPYEADRSDNVHRLNMGVFPVGAHIIPNPYNKIPGFTCHGAHGGGVHFV
                     PGFPVMAHPMVEWVLDTQYAHLFAKNAWVERSVIVFGAMEAALTPLMEDLERRFPVKV
                     FSLPSVDHPEYGRHIELGVKGSPVDVGSAYVQLKEGLVAQNMSLGPEVVRH"
     gene            complement(1849..2715)
                     /locus_tag="PKX82_06725"
     CDS             complement(1849..2715)
                     /locus_tag="PKX82_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HOF51983.1"
                     /translation="MNLLLDSFWRAAAYCLRPGIILLSLLPIVFMGLLTVGLGYFFWD
                     AAMDQVMLLMESSAFLQSAWQWLEGMGAGKLKSVIAPLIVIFGVTPLIVIVSMLVVAL
                     VMTPAMVRLVVRRRFAHLEAKNGATFLHSVVWSLGSTVLALVAMVISMPLWLVPPLVL
                     ILPPLIWGWLTYRVMAFDALATHASAEERRTLFREHRLWLMGMGVFAGYLGAAPSLVW
                     ASGVVFAAAFVVLLPMAIWIYTLVFAFSSLWFTHYCLSALHALRVQRAASSVAVDAAL
                     PPVPAPQHLEVL"
     gene            complement(2752..3708)
                     /locus_tag="PKX82_06730"
     CDS             complement(2752..3708)
                     /locus_tag="PKX82_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOF51984.1"
                     /translation="MDALTDGFSQLQLWLFEAVVQPLMFSLDLGNRLEDGFAGTGWLL
                     VGCLQLLVMLLVIAPLQKWRPVEPVTDRAAVRVDVLYTLIHRLGLFQIALFFTLEPLF
                     DALFSPLRAAGIATFHLDGLWPGVTDLPWVSFLLYLVVFDFLQYAIHRGQHQFTWWWS
                     LHSLHHSQRQMSMWTDNRNHLLDDVIRSAIVAVVAQGIGIAPGQFVAVVAFMQLCENF
                     QHANVRVWFGPIGERLLVSPRFHRVHHSIGIGHESAGKHTLGGHNFGVLFPWWDMLLG
                     TASFELKFEATGVRDQVEEGVDYGRGFWSQQWLGLKRLAGRR"
     gene            complement(3717..4532)
                     /locus_tag="PKX82_06735"
     CDS             complement(3717..4532)
                     /locus_tag="PKX82_06735"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOF51985.1"
                     /translation="MHHDSKPFSRTAIAIISIAVCTYSTGAGAQFSCANPVYLTFDTG
                     HMETAPLVADVLRKQQVKATFFVANERTKKGDGSLGNDWAFWWKAMAAQGHEFASHTY
                     DHVYWRGDIRGVKPTFRMQPTSGAFEGREFTWDAAKYCEQIGRAAERIQDHTGKKPLP
                     LFRAPGGKTSPRLLEVASACGYAHVGWAAAGFLGDELPSEKFSNEMLLKKALRDIRGG
                     DILMAHLGIWSRKDPWAPANLEPLIVGLKERGFCFETLRNHPDYRPWIASHGG"
     gene            complement(4516..>5257)
                     /locus_tag="PKX82_06740"
     CDS             complement(4516..>5257)
                     /locus_tag="PKX82_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOF51986.1"
                     /translation="LFVDPRTAQVQRTVRGILDPYQLRFSPDMKWFVTAGNRLNHLDI
                     YHWNGSDLTLAKRIATSKTPSHVWIDSTSTRLYASMQDSDELVMVNLATQTLKWRSKT
                     GSLPADVFGTPDDRFILVGLTGGTGVEVYDVTGDAPRLVKTIPTGEGAHAFRGVGDKR
                     HVFVSNRVANTISKIDYQTMAVVASYPAPGGPDCMDVSPDGRTIYVASRWAQYLTVID
                     TTTGKTVRRVKVGRSPHGVWTLDHAPRQ"
BASE COUNT         1171 a         1631 c         1507 g          948 t
ORIGIN      
        1 gcacgtgcat gccggaaccg ttgtcaccgt ggtagggctt gggcatgaag gtagccgtct
       61 tgccgtaggc attggccacg ttgtggatca cgtacttttg cagcatggtc cagtcagcgc
      121 gttccaccag ggtggagaaa cgtgtgccga tttcgttctg gccagcgccc gccacttcgt
      181 ggtggaacac ttcaaccggg atgcccaggg attcgaggat cagggacatc tcggcacgca
      241 tgtcctgcgt gctgtccaca ggaggaactg ggaagtagcc gcccttgacg gtaggacggt
      301 ggccgcggtt gccgccttcg agcttggagc cggtgttcca gggggcttcg tattcgtcga
      361 tttcgtagaa ggtgttgttg ggttcggtgc tccagcgcac gccgtcaaaa atgaagaatt
      421 ctggctctgg gccgaagaat gccgcgtcac caataccgga ggccttcagg taggcttcag
      481 cgcgcttggc aatggagcgg gggtcgcggt catacgactt gccgtcagca ggctccacca
      541 catcacagtt catgatgagg gtggtttctt caaagaacgg gtcgatgttg gcggtattgg
      601 gatcgggcat caacaacatg tcggatgctt cgataccctt ccagccggcg atggacgaac
      661 cgtcaaacgc gtggccggag gaaaacttgt cttcgtcgaa gtgcgacaca ggcaccgtgg
      721 tgtgctgctg cttgccacgg gtatcagtga aacggaagtc aacaaacttg acttcgttct
      781 ctttcaccat cttcattacg tctgcgacgg tcttggccat caaatactcc tgttacacgg
      841 ttatgaaaag aatctgcccc atcttcaatg cagcttttgt gccaaaaaat gcgccaaaga
      901 gatttcagag aaagttctgt attttgcctt gagaatggcc atttttcctc tgcgcaaagt
      961 caaagttgca tttatccgga gaccggccat cgcatccaca ccccacattg gggcgcacgc
     1021 aaagcaccat attggtgcaa tatcagtgac gcaccacttc agggcctaac gacatattct
     1081 gcgccaccaa cccctctttg agttgcacat aggccgaccc tacgtccacc ggacttcctt
     1141 tgacgcccaa ctcgatgtgg cgcccatatt ccggatggtc cacactgggc aggctgaaca
     1201 ccttgacggg aaagcggcgc tccaggtctt ccatgagcgg ggtcagcgcc gcctccatgg
     1261 caccaaacac gatgaccgag cgctcgaccc aggcgttctt cgcgaacaga tgggcgtatt
     1321 gcgtgtccag tacccactcc accatcgggt gggccatcac cgggaagcca ggaacaaaat
     1381 gcacaccgcc gccatgcgcc ccgtggcagg tgaacccggg aattttgttg tacgggttgg
     1441 gaatgatgtg cgcgcccacc ggaaacacgc ccatgttcaa ccggtgcacg ttgtcactgc
     1501 ggtcggcttc atagggcacc ccctgctcgc gggcgacgtc ctgcatgcgt tcacgaatca
     1561 gcgcttcagc ttcgcggtgc agttccagtt cgcaccccag agcccgggcg gcacactggc
     1621 gcgtgtggtc gtccggcgtg gcaccaatgc cgccaaacga gaacacgaca tcgcccgaag
     1681 cgaaggcacg cctgagcgtt gcggtgatgc ggtccggcgc atcgcccaca tagtcggcat
     1741 acgccacctg cagaccgcgt gcgcccagca cctcaatggc tttggccagg tgcttgtcag
     1801 tgcgtttgcc tgaaagaatt tcatcaccaa tgatgatgag tccgaaattc atagaacctc
     1861 cagatgttgc ggggcgggaa ccggcggcaa ggccgcatcc actgccaccg aagatgccgc
     1921 gcgttgcacg cgcagggcat gcaaggcgct caggcaatag tgggtaaacc acagtgatga
     1981 aaacgcaaaa accagcgtgt agatccagat cgccatgggc aacagcacca cgaatgccgc
     2041 ggcaaacacc acgccagagg cccacaccag gctgggggct gcacccagat agccggcgaa
     2101 cacacccata cccatcaacc acaaccgatg ctcccgaaat agggtacggc gctcctcggc
     2161 actggcatgc gttgccagcg catcgaacgc catcacacgg taggtcagcc agccccagat
     2221 cagtggcggc aggatcaaca cgaggggagg caccagccac aagggcatgg aaatgaccat
     2281 agccaccaat gccagcactg tggaacccag cgaccacacc acactgtgca gaaaggtcgc
     2341 accgtttttg gcttccagat gggcaaaccg gcggcggacc accaggcgga ccatcgccgg
     2401 cgtcatcacc aatgcgacga ccagcatgga cacaatcacg atcagcggcg tcaccccaaa
     2461 gatcacgatg agcggtgcaa tgacggattt gagcttgccc gcccccatac cctccagcca
     2521 ctgccaggca ctttgcaaaa acgcggacga ctccatcagg agcatgacct ggtccatcgc
     2581 agcatcccag aagaagtagc ccagccccac cgtcagcagg cccatgaaaa caatcggcag
     2641 cagcgacaga aggatgatgc ctggccgcaa acagtaagcg gcagcgcgcc agaaggaatc
     2701 aaggagcagg ttcatgcggc aagcataccg cggcgcagca aatgctgccg gtcagcgacg
     2761 cccagccagg cgcttgaggc ccagccattg ctgcgaccag aagccccgac cgtaatcgac
     2821 gccctcttcc acctggtcac gcacacccgt ggcttcgaat ttcagctcaa aactggccgt
     2881 acccagcagc atgtcccacc agggaaacag cacaccaaaa ttgtgcccac ccagggtgtg
     2941 cttgccagcc gactcatggc caataccgat gctgtggtgc acgcggtgga agcgcgggct
     3001 gaccagcagg cgttcgccaa tgggaccaaa ccacacgcgc acgttggcat gctggaagtt
     3061 ttcgcacaac tgcatgaagg ccaccaccgc cacaaactgc cccggcgcga tcccaatacc
     3121 ctgggctacc accgccacaa tggcactgcg aatcacgtca tcgagcaggt ggttacggtt
     3181 gtcggtccac attgacatct gccgctggga gtggtgcaag gaatgcagac tccaccacca
     3241 cgtgaactgg tgctgcccac ggtggatggc gtattgcaga aaatcgaaca ccaccaggta
     3301 cagcaaaaag ctgacccagg gcagatcggt cacacccggc cagaggccat ccagatggaa
     3361 agtggctatg ccagccgcgc gcaacgggga aaacagggcg tcaaacagcg gctccagggt
     3421 aaaaaacagg gcaatctgga acaacccaag acggtggatg agcgtgtaca gcacatccac
     3481 gcgcacagca gcccggtccg tcaccggctc gaccggtcgc catttctgca aaggcgcaat
     3541 gaccagcagc atgaccaaca actgcaggca cccgaccagc agccacccgg tacctgcaaa
     3601 gccatcttcc agccggttac ccagatccag cgaaaacatc agtggctgca ccacggcttc
     3661 aaacagccaa agctgcaatt gagaaaaccc gtccgtcaac gcgtccatca gctacctcat
     3721 cccccgtggg atgcaatcca tggccggtag tctgggtggt tgcgcagcgt ctcgaagcaa
     3781 aagccccgct ccttgagccc cacaatcagc ggctccagat tcgctggcgc ccaggggtct
     3841 ttgcgtgacc agatacccag atgggccatc aggatgtcac cgccacggat gtcgcgcagg
     3901 gctttcttga gcagcatctc gttgctgaat ttctcactcg gcaactcatc ccccagaaaa
     3961 ccggcagcgg cccagcccac atgtgcatag ccacaggcac tggccacttc cagcaaccgc
     4021 ggcgaagtct tgccaccggg tgcacgaaac aggggcaagg gcttcttgcc ggtgtggtcc
     4081 tgaatgcgct cggcagcccg tccaatctgc tcgcagtatt tggcagcatc ccaggtgaat
     4141 tcgcggccct caaaggcgcc cgatgtcggc tgcatccgga aggtcggttt gacccccctg
     4201 atatcgccac gccagtacac atggtcgtag gtgtgggagg caaactcgtg cccttgcgcg
     4261 gccatggcct tccaccagaa agcccagtcg ttgcccaggc tgccgtcgcc ttttttggta
     4321 cgctcgttgg cgacaaagaa ggtcgccttg acctgctgct tgcgcagtac atcggccacc
     4381 agcggcgcgg tttccatgtg gccggtatcg aaagtcagat acaccgggtt tgcacaggaa
     4441 aattgggctc cggcccccgt ggaatatgta cataccgcta ttgaaataat agcaattgca
     4501 gtacgggaga agggtttact gtcgtggtgc atggtccagt gtccagacgc cgtgcgggga
     4561 acggcccacc ttgacccggc gcacggtctt gccggtggta gtgtcaatca cggtcaggta
     4621 ctgggcccac cgcgaagcca catagatggt gcgcccatcg ggcgaaacgt ccatgcagtc
     4681 gggaccaccc ggcgcagggt agctcgccac caccgccatc gtctggtagt cgatcttgct
     4741 gatggtgttg gcaacccggt tactgacaaa cacatggcgc ttgtcgccaa ccccgcgaaa
     4801 ggcgtgcgca ccttcgccgg tcggaatggt cttgaccaga cgcggcgcgt cgccggtcac
     4861 gtcgtacacc tcgacaccgg tgccgccggt cagaccgacc agaataaacc tgtcgtccgg
     4921 cgtgccaaac acatcggcgg gcagtgagcc ggtcttgctg cgccatttga gggtttgcgt
     4981 ggccaggttc accatgacga gctcatcact gtcctgcatg ctggcataca gtctggtgct
     5041 ggtgctgtcg atccacacat ggctgggcgt cttgctggtg gcgatacgct tggccagggt
     5101 cagatcggac ccgttccagt ggtagatatc gaggtggttg aggcggttgc ccgctgtgac
     5161 aaaccacttc atgtccggtg aaaaacgcag ctggtagggg tccagaatgc cgcgtacggt
     5221 gcgttgcacc tgcgccgtac gtggatcgac aaacagc
//
LOCUS       DAOSJM010000155         5202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14229_length_5202_cov_4.074412, whole genome shotgun sequence.
ACCESSION   DAOSJM010000155 DAOSJM010000000
VERSION     DAOSJM010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14229_length_5202_cov_4.074412"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="PKX82_06745"
     CDS             complement(<1..1399)
                     /locus_tag="PKX82_06745"
                     /inference="COORDINATES: protein motif:HMM:NF006144.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOF51987.1"
                     /translation="MQYHLNGFKPGDPDVLAAAPDHWRAGDPLPEKVDVLIAGTGPAG
                     LCLAAQLAKVPQIRTMVVEPKLVPMEKGQADGINVRSMEMFQAFGFGEKMARESVWIN
                     ETTFWAPGTKAPLDRVARVQDVPDGISEMPHILINQARVHDMFLDVMRNAPTRLEPTY
                     GMKVQTLVIDPTAAEYPVTVTLEHTAPGREGQTATVRANYVVGCDGARSNVRAAIGGA
                     LHGDAAHQAWGVIDLLAVTDFPDWRMKSFVRSQDEGILMVLPREGGHLVRLYVELDAL
                     GEHERVAERGMNADDIIAKAKRILAPFTLEVKEVVWWSIYEIGHRLTDKFDDVPVAEM
                     ATRAPRVMLAGDACHTHSPKAGQGMNVSMGDTFNLGWKLISVLTGRADAALLHSYSGE
                     RRAAAKGLVEFDHKWARVVGGTATDDLAFDPSTAGLPVVARAFINNLPFTCGLTIQYE
                     PSALVGAATHQALATG"
     gene            1547..1954
                     /locus_tag="PKX82_06750"
     CDS             1547..1954
                     /locus_tag="PKX82_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOF51988.1"
                     /translation="MAGHLIRRLHQQSMQVFQTQTQAAGFDLTSVQFAALDAIAQQPG
                     IDQASLAATICFDRATIGGVIDRLERKGLVQRVVSAQDRRARLLHLTPEGEQLLAACQ
                     PVVKALQADILAPLSPAERDVFLALAQKALGLI"
     gene            1985..3283
                     /locus_tag="PKX82_06755"
     CDS             1985..3283
                     /locus_tag="PKX82_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HOF51989.1"
                     /translation="MERMQTPFRISPAAHGWLAPALLTLFALAGCTSTASIETQATAP
                     AMVLSNPIQAESAEIEHSQRFARWIADFSSSARTAGIHEATLHSAFDNVRFVPRVVEL
                     DRAQPEFTRAVWDYLDSALSKQRITLGKDRLLQLQSEANAVAARYGVPLEVLVAIWGI
                     ESNYGSNIGDIPTIDALATLGFEGRREAWARGQLLAALKILQNGDSTRAQMIGSWAGA
                     MGQTQFLPSAFLAYAVDADADGRRDIWGSVPDVMASTANFLARSGWQVGQPWGLEVRL
                     PEGFDYARADADVRQPATQWADEGVQSMDGAPLPSLADTAILLPAGACGPAFLVGPNF
                     RTVLRYNNATSYALAVNLLAQRLAGGLAVQAPWPRDLQLLARSQILALQAALNARGFD
                     TGTPDGLMGPATQRGIRQFQRSLGLPADGYPTLDLLQRLQ"
     gene            complement(3297..3632)
                     /locus_tag="PKX82_06760"
     CDS             complement(3297..3632)
                     /locus_tag="PKX82_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51990.1"
                     /translation="MEYEERFTDAELGQVIEQAMIYMCACPAQVADAVRKPRELYRYQ
                     LRCIADPGNNSAVHSLIAQTTIHSHAIMQERLEKVIELEEWDRSTLEMPAGLRKRQLQ
                     EILADQDGK"
     gene            complement(3698..4342)
                     /locus_tag="PKX82_06765"
     CDS             complement(3698..4342)
                     /locus_tag="PKX82_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOF51991.1"
                     /translation="MARRTKEDALVTRDLILDAAERVFQRRGVSRTSLQEIAQDAGLT
                     RGAIYWHFQNKSDVFDAMMQRVTLPMAATLQAPPEAEAADPLQHLRRTLASAFHQTVH
                     DAQVQRVFEIASHKVEYVDELQALRERHIADRDSCLSDLNRLLALAVERKQLSAGVPV
                     RVATIGLHALIDGLTHNWLLDPQAFDLETTGAQAVDAYLAGLAVSRCTEQQRTR"
     gene            4518..>5202
                     /locus_tag="PKX82_06770"
     CDS             4518..>5202
                     /locus_tag="PKX82_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOF51992.1"
                     /translation="MSSLRPSVARTFRSIPLHRYALVLTPLALLVACGAKDAAPTAGA
                     PSGPPPATVGVVTVASEAVALQTELPGRVSAVRVAQVRARVNGVVQKRLFREGSEVTA
                     GQPLYQIDPAPYRAALESAQASVAKSQANLAQATAQVERYKPLVEANAISKQEYTTLQ
                     TTQKQAEADLATARAAVQTAQINVDYASVRAPISGRIGQALVTEGALVSATEATQLAL
                     IQQTGSVYVN"
BASE COUNT          967 a         1650 c         1596 g          989 t
ORIGIN      
        1 acccggtggc cagggcctgg tgcgtggccg cgccaacgag ggcactgggt tcgtactgga
       61 tggtcagacc gcaggtaaac ggcaggttgt tgatgaaggc ccgggccacc accggcagac
      121 cagcggtcga cggatcgaaa gccaggtcat cagtagccgt gccgcccacg acccgcgccc
      181 atttgtggtc aaactccacc aggcccttgg ctgcggcgcg acgctcgccc gaatagctgt
      241 gcagcagtgc ggcgtcggcg cggccggtga gcaccgagat cagcttccag cccaggttga
      301 aggtgtcgcc catcgacaca ttcatgccct gcccggcctt ggggctgtgg gtgtgacagg
      361 cgtcgccagc cagcatcacg cggggtgcgc gtgtggccat ctcggccacg ggcacgtcgt
      421 caaatttgtc ggtcaggcgg tgcccgatct catagatgga ccaccacacc acttctttca
      481 cctccagcgt gaagggtgcc agaatccgtt tggccttggc gatgatgtcg tccgcgttca
      541 tgccccgttc agccacacgc tcatgttcgc ccagtgcatc gagttcgaca tacaggcgca
      601 ccaggtgtcc gccctcgcgc ggcagcacca tcaggatgcc ctcgtcctgc gagcgcacaa
      661 aggacttcat gcgccagtcg ggaaaatcgg tcacggccag cagatcgatg acgccccagg
      721 cctggtgcgc cgcgtcgccg tgcagcgctc cgccaatggc ggcgcgcacg ttggagcgcg
      781 cgccgtcgca gccaaccaca tagttggcac gcaccgtggc ggtctggccc tctcggcccg
      841 gcgcggtgtg ctccagggtc acggtgacgg ggtactcggc ggcggtgggg tcgatcacga
      901 gggtctgcac cttcatgccg taggtgggct ctagccgcgt gggggcgttg cgcatgacgt
      961 cgaggaacat gtcatgtacg cgcgcttggt tgatcaggat gtgcggcatc tcggaaatgc
     1021 catcgggcac gtcctgcacg cgtgccacgc ggtccagcgg tgctttcgtt ccgggcgccc
     1081 agaaggtggt ctcattgatc cagaccgact cgcgcgccat cttttcgccg aaaccgaagg
     1141 cctggaacat ttccatggag cgtacgttga tgccgtcggc ctggcccttc tccatcggca
     1201 ccagtttggg ctccaccacc atggtgcgaa tctgcggaac cttggccaat tgggcggcca
     1261 ggcacaaacc agcgggcccg gttccggcaa tcagcacatc aactttctct ggcagcgggt
     1321 cccccgcacg ccagtggtcc ggggctgccg cgagaacatc agggtcgcca ggtttgaatc
     1381 cgttcaggtg gtattgcatg gtgtctccct caggcggttc gccaagttgt taacgatatg
     1441 tatgcatatc atattaatac atattacaat tgtcaagcgg tcggacgaag cacatacaac
     1501 agggcgcggt atgcaaaaaa cagaaacatg gagcgaagga accaggatgg ccgggcacct
     1561 gatccggcgc ttgcatcagc aatcgatgca ggtctttcag acccagacgc aggccgcggg
     1621 gttcgatctg acctcggtgc agtttgccgc tctcgacgcc attgcccagc aacccggcat
     1681 cgaccaggcc agtctcgccg cgaccatctg cttcgaccgc gccacgatcg ggggagtgat
     1741 tgaccgcctg gaacgcaagg gcctggtgca gcgtgtggtc agcgcgcagg atcgtcgggc
     1801 acgcctgctt cacctcacgc cggagggcga gcaattgctg gcagcctgtc agcccgtggt
     1861 gaaagcactg caggccgaca tcctggcgcc cctgtcgccc gcggagcgtg atgtcttcct
     1921 ggcactggcg caaaaagcgc ttggcttgat ctgataccca ggaaaaatgc cgcaaccaac
     1981 gagaatggag cgaatgcaaa cacccttccg gatttcccca gcagcccacg gctggctggc
     2041 tcctgcgtta ctgacactct tcgcacttgc gggttgcacc tccacagcct ccatcgaaac
     2101 ccaggccacg gcaccggcca tggttctgtc caatccgata caggcggaat ccgccgagat
     2161 cgagcacagt cagcggtttg cacgctggat agccgatttc agctccagcg cacgcacagc
     2221 gggcatccac gaagcaacgc tccatagcgc tttcgacaac gtgcgctttg tcccgcgcgt
     2281 cgttgagctg gatcgggccc agcccgagtt cacccgtgca gtgtgggact acctcgacag
     2341 cgcgctgtcg aagcagcgca tcacccttgg caaggacagg ctgctgcaac tgcagtcgga
     2401 agccaatgcc gtagcagcgc gctacggcgt cccgctggag gtccttgtgg cgatctgggg
     2461 catagagagc aactacggca gcaacatcgg tgacatcccc accatcgatg ccctggcgac
     2521 gctcgggttt gagggccgcc gcgaagcatg ggcccggggt cagttactcg cggctctgaa
     2581 aatcctgcaa aacggcgata gcacccgcgc acagatgatc ggatcgtggg ctggtgcaat
     2641 gggccagacg cagtttctgc catcggcctt tttggcttat gcagtggatg cggatgcgga
     2701 tggacggcgt gatatttggg gcagcgtgcc cgatgtgatg gcctcgactg caaatttcct
     2761 tgcccgctcg ggctggcagg tgggccagcc ctgggggctg gaggtgcgtt tgccagaggg
     2821 ttttgactac gcgcgcgccg atgccgatgt gcggcagcca gcaacccaat gggcagatga
     2881 aggtgtgcaa tccatggatg gcgcaccgtt gcccagtttg gctgacacgg ccatcctgct
     2941 cccggctgga gcatgcgggc cggccttcct ggtgggaccg aactttcgca cggtcctgcg
     3001 gtacaacaat gcgaccagtt atgcactggc cgtcaacctg ctggctcagc gactggctgg
     3061 tggcctggcg gttcaggctc cgtggccacg cgacttgcag ctgcttgcgc gcagccagat
     3121 actggcgttg caggccgctt tgaacgcacg tggatttgac accggtacac ccgatgggct
     3181 gatgggaccg gccacacagc gcggcattcg gcaattccag cgcagcctgg gccttccggc
     3241 agatggctac ccgaccctgg acctgttgca gcgcctgcag tgaatcatgg agacggctat
     3301 ttgccgtcct ggtcggcaag gatctcctgc agctgccgct tgcgaagacc ggctggcatc
     3361 tccagtgtgg acctgtccca ttcctccagc tcgatcacct tctccaggcg ctcctgcatg
     3421 atcgcatgcg aatgaatggt tgtttgtgca atcaaggaat ggactgcact gttgttccca
     3481 gggtccgcaa tacaccgaag ttgatagcga tacaactccc tgggcttgcg cacggcgtcg
     3541 gccacttgtg caggacaagc acacatgtag atcatggcct gctcgatcac ctgacctagt
     3601 tcagcatctg tgaaacgctc ttcgtattcc atgacttgaa ttccttgatc gacattttgc
     3661 acaacactgg caaaagatga agtaaatttt cctgctatca ccgcgtgcgt tgttgctctg
     3721 tgcagcgaga gacagccaat cctgccaggt aggcgtccac cgcctgggcg cccgtggtct
     3781 ccagatcaaa agcctggggg tcgagcaacc agttgtgcgt caggccgtca atcagggcgt
     3841 gcaggccgat cgtcgccaca cgcacaggca cgcctgcgga cagctgtttg cgctcgacgg
     3901 ccagtgccag caggcggttc aggtcactca ggcaactgtc gcggtctgca atatggcgct
     3961 cgcgcaacgc ctgcagctca tccacatact cgactttgtg cgaggcaatc tcaaacaccc
     4021 gctgcacctg tgcgtcgtgc accgtctggt gaaaggcgga agccagtgtg cgccgcaggt
     4081 gctgcaaagg gtcggcagcc tcggcttccg gcggggcctg gagcgtcgcc gccatgggca
     4141 acgtgacgcg ctgcatcatg gcgtcgaaga cgtcggactt gttctggaaa tgccagtaaa
     4201 tggccccgcg cgtcaggccc gcatcctggg caatctcctg cagcgaggtg cgggaaacac
     4261 ccctgcgctg aaacacccgc tccgccgcgt ccaggatcag gtcacgggtc actaaggcgt
     4321 cttctttggt gcgtctggcc atatctcgct tgctgtcagg gttatttact gagcgcacac
     4381 aatatacata cattcatgta tgtatactaa accaaatttc aagtaccact agctttcgtt
     4441 gacgccggtt tgcgctggct caacatccac cttccctctg gcacctgcca gacttctcca
     4501 ggaaacaagg atttttcatg tcctcattgc gcccatccgt tgcccggact ttccgctcca
     4561 ttcccctgca ccgttatgcc ctggtgctga cgcccctggc cctattggtg gcttgcggcg
     4621 ccaaggatgc cgcgcccacc gcgggtgccc cctctggccc gccgccagcc actgtcgggg
     4681 tggtcaccgt ggccagtgag gccgtggcgc tgcagaccga gctgccgggc cgtgtcagtg
     4741 cggtacgcgt ggcgcaggtc cgtgcacggg tcaatggtgt ggtgcaaaag cgcttgttcc
     4801 gggagggcag cgaagtcacg gccggccagc cgctctacca gattgatccc gcaccctacc
     4861 gggcggcgct ggagagcgcc caggccagtg tcgccaaaag ccaggccaat ctggcacaag
     4921 ccaccgcaca ggtggagcgt tacaagcccc tggtggaagc caacgccatc agcaagcagg
     4981 aatacaccac attgcaaacc acccagaagc aggctgaggc ggacctggcc actgcccgtg
     5041 ctgcagtgca gactgcacag atcaacgtgg attacgccag cgtccgcgcg cccatttccg
     5101 ggcgcattgg ccaggccctg gtgaccgaag gcgcattggt gagcgcgacc gaggccaccc
     5161 agctcgccct gatccagcag accggctcgg tgtatgtgaa ct
//
LOCUS       DAOSJM010000156         5137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14477_length_5137_cov_4.049980, whole genome shotgun sequence.
ACCESSION   DAOSJM010000156 DAOSJM010000000
VERSION     DAOSJM010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5137
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14477_length_5137_cov_4.049980"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PKX82_06775"
     CDS             complement(<1..1321)
                     /locus_tag="PKX82_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF51993.1"
                     /translation="MPLFRPGHALLLLIVGLLMLPVLAVLASWLPLGAPLAASWSILR
                     EMTATVLPEYMGTTVVLCVLVAVGVTVFGLGTAAAVTLFDFPGRRFFEWVLLLPLAMP
                     AYVVAYAYSDFLQYSGPLQTAMRSLLGAEGRMLPEVRSVWGAAWVFTFALYPYVYLLS
                     RAALGERASHLMEAARLMGAPMARRIREVAFPLARPAVAAGVALALMETLADFGVSSY
                     FGIQTFTAGIYKAWLSMDNRIAAAQLSTVLLVLVALLLGLEQRAQKRMRFSAVKGARA
                     GAADAQAMVLTGRRAMLAWALCLLPVLMGFVLPILFMLRPMLLGWDELPWERFGQWAL
                     NSLRLASITSALAVAVALGIAFSLRQSSHWAPRVAARLASLGYAIPGAVVVVGLLLPV
                     GWLQDRFPSLQVGYFITATALGIVWAYLVRFCAVALQSLQSGYARVPQ"
     gene            1390..2457
                     /locus_tag="PKX82_06780"
     CDS             1390..2457
                     /locus_tag="PKX82_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF51994.1"
                     /translation="MFLEVSQLTVSYPGRSRPAVRDVSLGLRAGDIGVLIGPSGCGKT
                     TLLRAVAGLEMPSAGSISLSKHIVSDAHTRVPAEQRRIGMVFQDYALFPHLDVGRNVA
                     FGIAHLPVAERAARVAEVLQWVGLEGSDKHYPHALSGGQQQRVALARALAPKPQLLLL
                     DEPFSNLDVDLRERLAHEVRGILKAAGATALFVTHDQLEAFAIGDMIGIMHEGQLHQW
                     DDAYTLYHRPATRFVANFIGHGVFAPAHILERHGEVVVETPLGELRGVAECPLPNAFP
                     HGECDILLRADDIVHDDAAPVKAEITRKAFRGSEFLYTLRLASGLTVMAHVPSHHDHS
                     LGEWIGIRPQVDHVVTFRREA"
     gene            complement(2441..3193)
                     /locus_tag="PKX82_06785"
     CDS             complement(2441..3193)
                     /locus_tag="PKX82_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOF51995.1"
                     /translation="MKQLVALGRLLRVVQHLLQGVWIIRRAFPKLSPTEREERVQAWA
                     RAMLAHLAIKLVVNGQPPLAGPVLLVANHISWLDIVVMHAARHCRFVSKADIQRWPVV
                     GTLADAAGTLYIERESRRDALRVVHHMAERLQAGDVLAVFPEGTTGDGAHVMPFHANL
                     LQAAISAQAPVQPVALHFADCVTGAMSFAPCYIGDDTLLQSVWRTLTTPNLEAVVSFG
                     VPQQADGRDRRTWATDLRTAVTDLRADPRLRV"
     gene            complement(3210..4502)
                     /locus_tag="PKX82_06790"
     CDS             complement(3210..4502)
                     /locus_tag="PKX82_06790"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOF51996.1"
                     /translation="MKILIQGGRVIDPASGFDEKADVALANGAVVAIKNVANDFVPDQ
                     VMDATGCIVMPGLVDLCARLRESGRPRAGELSSERAAAAAGGVTTLVCPPDTTPVLDE
                     AGLVEMLHYRTQLQGGPRVLPLGALTRALAGEVLAPMQQLADAGCVGFGQAEVPLANT
                     QVLQRALQYASTFGYPVWLRPQEMYLGKGVAASGPLATRLGLSGVPVAAETIALHTIL
                     ELVRTTRARVHLCRISSAAGVELVRQAKAEGLPVSCDVSINSLHLTDLDIGYFDSRMR
                     LNPPLRQQRDRDALRAALVDGTIDALVSDHTPVDDDAKALPFAEAEPGATGMELLLSL
                     ALKWAQDSGVPLARAMAVVTSQPAGVLGASTGALASSLGRMTVGAVADLCIFDPQAAW
                     VVEPSALRSQGKHTPFGGYELPGRVRTTLVSGKVAFAA"
     gene            complement(4499..>5137)
                     /locus_tag="PKX82_06795"
     CDS             complement(4499..>5137)
                     /locus_tag="PKX82_06795"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOF51997.1"
                     /translation="AMAADMFVVRHSESGAPYLIAQHVAPHVHVINAGDGRHAHPTQG
                     LLDMFTIRHYKKDFSNLTVAIVGDVLHSRVARSDIHALTTLGAAEVRVVGPKTLVPSD
                     MAQMGVRVCHTLEEGIKDCDVIIMLRLQNERMSGALLPSMHEFFKSFGLTPEKLLLAK
                     PDAIVMHPGPINRGVEIDSAVVDGKQSVILPQVTFGIAVRMAVMSIVAGNEA"
BASE COUNT         1055 a         1721 c         1541 g          820 t
ORIGIN      
        1 tctgcggcac acgcgcgtaa ccactctgca gggactgcag ggccaccgcg caaaaccgca
       61 ccaggtaggc ccagacaatg cccagcgccg tcgcggtaat gaagtaaccc acctgcaggg
      121 acggaaaccg gtcctgcaac cagcccaccg gcaacagcag ccccaccacc accaccgcac
      181 cggggatggc ataacccagg ctcgccagcc gggcagcaac acgcggcgcc cagtggctgc
      241 tctggcgcaa gctgaacgca atgcccagcg ccacagcaac ggccagggcc gaggtgatgc
      301 tggccaggcg caggctgttc agtgcccatt gcccaaagcg ctcccagggc agttcatccc
      361 accccaacag catgggtcgc agcatgaaga gaatcggcaa gacaaagccc atcaacaccg
      421 gcaacaggca cagtgcccag gccagcatgg cgcgccgtcc ggtcagaacc atcgcctgtg
      481 catcggctgc accggcgcgc gcacctttca cggctgagaa ccgcatgcgc ttttgtgcac
      541 gctgctccag acccagcagc aatgccacca gcaccagcaa taccgtagag agctgcgccg
      601 cggcgatacg gttgtccatg gacagccatg ccttgtatat gccagcagta aatgtctgaa
      661 tcccgaaata gctcgacacg ccgaagtctg ccagtgtctc catcagggcc aacgccacac
      721 cggctgccac cgcagggcga gccaggggga aagctacttc ccggatacgc cgcgccatgg
      781 gtgcacccat caggcgggcc gcttccatca gatgcgatgc acgctccccc agcgcagccc
      841 gcgacagcag gtaaacatag ggatacagcg caaaggtaaa cacccaggcc gcaccccaga
      901 cgctgcgcac ctcgggcaac atgcgccctt ctgcgcccag cagactgcgc atggcggttt
      961 ggagcggtcc gctgtattgc aggaaatcac tgtaggcata ggccaccaca taggccggca
     1021 tggccagcgg caacaacagg acccattcga agaagcgccg ccccggaaaa tcaaacaggg
     1081 ttacggccgc tgctgtgccc aggccaaaca cagtgacacc cacagccacc agaacacaca
     1141 agaccaccgt agtgcccatg tattcgggca gcaccgttgc ggtcatctcg cgcaggatgc
     1201 tccatgacgc ggccagcggg gcgcccagtg gcagccacga tgccaacacc gccagaacgg
     1261 gcagcatcag caagccgacg ataagcagca acagcgcgtg accaggacga aaaaggggca
     1321 tgggaaagaa gctcggggac agcaggacta aatgagaatt gtatgcattt gggatctaca
     1381 atccggaaca tgtttctaga ggtctcccaa ctcactgttt cgtacccggg ccgcagcagg
     1441 cctgccgtgc gcgatgtgtc gctgggtttg cgtgcaggcg acatcggcgt actcatcggc
     1501 ccctcgggct gcggcaagac aaccctgcta agggccgtag ccggactgga gatgcccagt
     1561 gcaggatcga tttccctgtc caagcacatc gtcagcgacg cccacacccg cgtgcccgcg
     1621 gagcagcgcc gcattggcat ggtgtttcag gactatgccc tgtttccgca cctggacgtg
     1681 ggccgcaacg tggctttcgg catcgcccac ctgcccgtgg cagaacgggc tgcccgcgtg
     1741 gccgaagtgc tccagtgggt aggactggaa ggctccgaca agcactaccc ccacgcactg
     1801 tccggcggac agcagcaacg cgtggcgctg gcacgtgcac tagcccccaa gccgcaactc
     1861 ctgttgctgg acgaaccttt ttctaatctg gatgtggacc tgcgcgagcg cctggcgcac
     1921 gaagtacgcg gcatcctgaa agcggcaggc gccacggcgc tgtttgtcac ccacgaccag
     1981 ctggaagcct ttgccatcgg cgacatgatt ggcatcatgc acgaggggca attgcaccag
     2041 tgggacgacg cttacacgct gtaccaccgg cccgctacgc gctttgtggc caactttatc
     2101 gggcatggtg tctttgcccc cgcgcatatc ctggagcgtc acggcgaagt agtggtggag
     2161 acgccgctgg gtgaactccg cggtgtggct gaatgcccgc taccgaatgc atttccccat
     2221 ggtgaatgtg acatcctgct gcgcgccgat gacatcgtgc acgatgatgc cgcgccggta
     2281 aaggcggaaa tcacgcgcaa ggcttttcgg ggttcggaat ttctctatac gttgcggctg
     2341 gccagcggac tgaccgtcat ggcccatgtc cccagccacc acgaccattc attgggcgaa
     2401 tggatcggca tccgcccgca ggtggaccat gtggtgacgt tcagacgcga agcctagggt
     2461 ccgcacgcaa gtccgtcacg gctgtgcgca agtcggtagc ccaggtgcgg cggtcgcgcc
     2521 cgtctgcctg ctgcggcacc ccaaagctga ccacggcttc cagatttggc gtggtgagcg
     2581 tgcgccacac ggattgcagc aaggtatcgt cgccgatgta gcacggggcg aaactcatgg
     2641 cacctgtcac acagtcggca aagtgcagcg ccaccggctg caccggcgcc tgggccgaaa
     2701 tggccgcttg cagcagattc gcgtggaacg gcatgacatg ggcaccatcc ccggtggtgc
     2761 cttcgggaaa caccgccagc acatcgcccg cctgcagtcg ctcggccatg tggtgcacca
     2821 cccgcagcgc atcccggcgg gactcacgtt cgatatacag cgtgccggcg gcatcggcca
     2881 acgtccccac caccggccag cgctgtatgt ccgccttgga cacaaagcgg cagtggcgtg
     2941 cggcatgcat caccacaatg tccagccacg agatgtggtt ggccaccagc aataccgggc
     3001 ccgccagagg cggctgtcca ttcactacca atttgatagc taggtgtgca agcatggcgc
     3061 gggcccaagc ctgaacacgc tcttcacgtt ccgtcggaga cagcttgggg aacgcacggc
     3121 ggatgatcca tacgccttgc agcaggtgct gcaccacgcg caataaccgg cccaaagcca
     3181 ccaactgttt catgggcggg atgctcaggt caggccgcaa aggcgacttt gccgctgacc
     3241 agggtggtgc gcacacgccc gggcaactcg tagccgccaa acggtgtgtg cttcccctgg
     3301 ctgcgcaatg cactgggctc cacaacccat gccgcctgcg gatcgaagat gcacaggtcg
     3361 gctacggccc cgacagtcat gcgccccagg ctggaggcaa gtgcccccgt ggaagcgccc
     3421 agcacacccg cgggctggct ggtcaccacc gccattgcac gggccagcgg cacgccgctg
     3481 tcctgggccc acttgagtgc caaactcagc aacaactcca tcccggtcgc acccggctcg
     3541 gcctcggcaa acggcaaggc cttggcatcg tcgtccaccg gagtgtggtc cgacaccagt
     3601 gcgtcaatcg ttccatccac cagcgcagca cgcagggcat cgcggtcgcg ttgctggcgc
     3661 aacggtgggt tgaggcgcat acggctgtca aagtaaccga tatccaggtc cgtcagatgc
     3721 agcgagttga tgctgacatc gcagctcact ggcaagcctt cggccttggc ctggcgcacc
     3781 agttcgacac cggcagcact gctgatgcgg cacagatgca cgcgcgcacg cgtggtgcgc
     3841 accagttcca ggatggtgtg cagtgcaatc gtctctgccg ccacgggaac accgctcaag
     3901 ccaagccggg tggccagcgg accgctggcg gccacaccct tgcccaggta catctcctgg
     3961 ggccgcagcc agactgggta gccaaaggtg ctggcgtatt gcagggcgcg ctgcagtacc
     4021 tgcgtattgg ccagtggaac ttcggcttgg ccaaaaccca cgcagccggc atccgccagt
     4081 tgctgcatgg gcgcgagcac ctcgccagcc agcgcacgcg tgagcgcgcc cagcggcagc
     4141 acacgcgggc caccctgcag ctgggtacgg taatgcagca tttccaccag accagcttcg
     4201 tcgagcaccg gcgtggtgtc gggcggacac accagggtgg tgacgccacc agccgcagcc
     4261 gcggcgcgct cgctgctgag ctcgccagcc cgagggcgac cagactcacg caggcgtgca
     4321 cacaggtcca ccagaccggg catcacgatg caaccagtgg catccatcac ctggtccggc
     4381 acaaagtcgt ttgctacatt tttaatagct accaccgcac cattggcgag ggctacatcg
     4441 gctttttcat caaaaccact ggccggatcg atgacacggc cgccctggat caatatcttc
     4501 atgcttcatt ccccgcaacg atgctcatca ctgccatgcg tactgcaata ccaaacgtca
     4561 cctgcggcag gatcacgctc tgcttgccgt ccaccaccgc agaatcaatt tccacaccgc
     4621 ggttgatggg accggggtgc atcacgatgg catcgggctt ggccagcagc agcttctcgg
     4681 gcgtgaggcc aaagctcttg aagaactcat gcattgaagg cagcagcgca ccgctcatgc
     4741 gctcgttctg caggcgcagc atgatgatga cgtcgcagtc cttgatacct tcttccagcg
     4801 tgtggcagac ccgtacgccc atctgggcca tgtcggacgg caccagcgtc ttgggtccca
     4861 ccacgcgcac ctcggcagca cccagggtgg tcagggcatg gatatcagaa cgcgccaccc
     4921 gcgaatgcag cacatcgccc acgatggcca ccgtaaggtt ggaaaagtcc ttcttgtagt
     4981 ggcggatggt gaacatgtcc agcaacccct gtgtggggtg ggcatggcgg ccgtcaccgg
     5041 cgttgatcac atgcacatgg ggcgccacgt gctgggcgat caggtaaggc gcgcccgatt
     5101 cactgtggcg cacgacaaac atgtcggccg ccatggc
//
LOCUS       DAOSJM010000157         5136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14486_length_5136_cov_3.511317, whole genome shotgun sequence.
ACCESSION   DAOSJM010000157 DAOSJM010000000
VERSION     DAOSJM010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14486_length_5136_cov_3.511317"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PKX82_06800"
     CDS             complement(<1..226)
                     /locus_tag="PKX82_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51998.1"
                     /translation="MKLSRTILGFLTFSALAGVLATAFAAGLAAALGAGLDAGLATGL
                     AVLVFAGAFFVAGFATAFAALAGAAMTGFAG"
     gene            228..416
                     /locus_tag="PKX82_06805"
     CDS             228..416
                     /locus_tag="PKX82_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF51999.1"
                     /translation="MPKAEFAVIDALKVRATNLKKSVKKTELLRAGIKALAAMSDTAF
                     LAAVTAVPNIKTGRPSKS"
     gene            complement(431..892)
                     /locus_tag="PKX82_06810"
     CDS             complement(431..892)
                     /locus_tag="PKX82_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOF52000.1"
                     /translation="MDLILWRHAEAIDLELVGDDMERTLTSRGEKQAARMAAWLDRQL
                     PDGAKVLASPAVRTEQTARALGRKFKTHAALAPLATPEQLLELVQWPHAKGCYVVVGH
                     QPTLGQTIARLIGLTETECAVKKGAIWWLRYRERDAEGHTVVVTVQSPDLL"
     gene            complement(898..3000)
                     /gene="ppk1"
                     /locus_tag="PKX82_06815"
     CDS             complement(898..3000)
                     /gene="ppk1"
                     /locus_tag="PKX82_06815"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOF52001.1"
                     /translation="MLSFFGKSKEQPQQGAPDLLDRDHSILAFNERVLSWAVRDDVPL
                     LERLRYLCIVSSNLDEFFEVRAEPHLVAHMRHDRKGTYTVETFNRLAAALQSLVARQY
                     AIYNDKLLPAFEAEGIKVISHGERNSAQRAWVKQYFEREVRPLLIPVGLDPSHPFPQV
                     ANKSLNFIVRLGGKDAFGRENEIAIVKVTRVLPRFIRLPEKVSGKRALFVSISSVIRA
                     HLGELFVGRTVGQFSQFRVTRHSDLSVDEEDVKNLRTALRQGLVHRHFGQAVRLEVSA
                     GCSQYLSDFLLEQFELPQESLFRVPGPVNLVRLTQLIDLADRSDLCFSSYKAAPAQQL
                     VAGQSIFDQLKQRDIFIHQPFESFDGVLTFLREAVHDPQVLAIKQTIYRTGADSELMD
                     LLREAVRQGKEVTVVVELKARFDEEANINWAEMLESIGAQVVYGVVGLKTHAKMMLVT
                     RREGKLLKRYGHLSTGNYNPRTAKLYTDVSHITADAALTTDMEHVFVHLASQSRLPRL
                     SKLWMAPFHLHRNLVAKIDALGKAAEKGRSCRIVAKMNSLTDDALIASLMRAGKCGVK
                     IDLIVRGACMLPAQVPGHTDNIRVRSIIGRFLEHSRVFYFRDGESEDLYLSSADWMNR
                     NMVRRVELAWPVTDEQQKRRIVDECLVAYLHDTVDAWELGPDGTYTRITANKGPAHGA
                     QAALVARYGRNTKAQRGG"
     gene            complement(3040..4158)
                     /locus_tag="PKX82_06820"
     CDS             complement(3040..4158)
                     /locus_tag="PKX82_06820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOF52002.1"
                     /translation="MKLALITDAWQPQVNGVVTTLVELVREMDALDHQVTVIHPGLFR
                     TRPCPGYDGIDLAVRPYAQLASQLDALAPEAIHIATEGPLGWAARRYCLRRKLAFTTA
                     YHTRFPEILKAALKVPLWLGYAVFRHFHKPSSGVLVPTQGVLRLLEARGFRHLRGFRH
                     LRGFRHLRGWTHGVDMGLFSHHPQATECVHLGNLTRPVSLFVGRVSYEKNIEAFLQLQ
                     VPGTKVVCGVGPLEAALKERYSGVYWLGVLPRAELASVYAAADVFVMPSQSETFGLVM
                     LEAMASGTPVAAFPVDGPLEVLGSPAQGGALNTDLSLAWSDALAVPRVEARSRALTFS
                     WAHASRLFVGHLVPAQNQSRTGNSSVCAQTVTQMSSKP"
     gene            complement(4155..5027)
                     /locus_tag="PKX82_06825"
     CDS             complement(4155..5027)
                     /locus_tag="PKX82_06825"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOF52003.1"
                     /translation="MRTTFDALGTNWQGLAPGAEDEVDHAHGTHRYRAVFISDIHLGT
                     AGCQAQALLEFLKHHPSDYLYLVGDIVDGWQLRRRWFWPQAHNDVVQKLLRSARKGSR
                     VVFIPGNHDEFARGFVGHHFGGVEVCEDTVHTTADGRTLWITHGDYFDGVIQCAKWLA
                     YLGDNAYEFTLKLNRYLNSLRARMGLPYWSLSAYLKHKVKKALNYVTDFEVAVAAEAR
                     SRGHDGVVCGHIHRAEMRTINGTLYCNDGDWVESRSALVEHMDGRLELVYWDDVLSSQ
                     FREPSAPQPMGVVA"
     gene            complement(5028..>5136)
                     /locus_tag="PKX82_06830"
     CDS             complement(5028..>5136)
                     /locus_tag="PKX82_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine-acyl-ACP acyltransferase"
                     /protein_id="HOF52004.1"
                     /translation="LGAPAWDPDFNSADLPMLMRIADLPSRYRKHFLGA"
BASE COUNT         1091 a         1664 c         1439 g          942 t
ORIGIN      
        1 cacccgcgaa acccgtcatt gctgcaccag ccaaagcagc aaaagcagta gcaaaaccgg
       61 ccacaaagaa ggcaccagca aaaacaagca cagccaaacc tgtcgccaag cctgcatcca
      121 aacccgctcc taaagccgct gccaaaccgg ctgcaaaggc cgttgccaag actccagcca
      181 aggcagaaaa agtcaaaaaa cccaagatgg tacgtgacag tttcactatg cccaaggccg
      241 aattcgcagt cattgatgca ctcaaagtcc gtgctaccaa tctcaaaaaa tccgtcaaga
      301 agaccgagct gctgcgtgcg ggcatcaagg cactggctgc catgtccgat actgcattcc
      361 ttgctgccgt aaccgccgtg cccaatatca agaccggccg tccttccaag agctgagttc
      421 cgacgcagcg ctagagcaga tcgggggact gcaccgttac tacaacggtg tgtccctctg
      481 catcacgctc ccggtagcgc agccaccaga tggcgccctt cttgacagcg cattccgttt
      541 cggtcagtcc gatcagccgg gcaattgtct gccccagcgt gggctggtga ccaacgacca
      601 cgtagcagcc ctttgcatgg ggccattgca ccagctccag cagttgttcc ggggtagcca
      661 gtggcgccag cgccgcatgg gtcttgaact tgcgccccaa cgccctcgcc gtctgctcgg
      721 ttcgcacagc cgggctggcc agcactttgg cgccgtctgg caactgccga tccagccagg
      781 ccgccatgcg ggcagcctgc ttttccccac gtgaagtcag ggtgcgctcc atgtcgtcac
      841 ccaccagttc cagatctatg gcctctgcgt gccgccacaa aatcaaatcc atgtcgatca
      901 ccctccgcgt tgtgccttgg tattacgtcc atagcgcgcc accagtgctg cctgcgcgcc
      961 atgcgcaggc cccttgttcg ctgtgatgcg ggtgtaggtg ccgtctggac cgagttccca
     1021 ggcatccacc gtatcgtgca ggtaagccac cagacattca tccacgatac ggcgtttttg
     1081 ctgttcatcg gtgacgggcc aggccagttc cacacgccgc accatgttgc ggttcatcca
     1141 gtcggcactc gacagataga ggtcttccga ttcgccatca cggaaataga acacgcggga
     1201 gtgttccagg aaacgcccga tgatcgaacg cacccggatg ttgtcggtgt gccctggaac
     1261 ctgcgccggc agcatgcacg cgccgcgaac aatcaggtca atctttacac cacatttgcc
     1321 agcgcgcatc aggctggcaa tcaacgcgtc gtccgtcagg gagttcatct tggccacgat
     1381 ccggcatgac ctgccttttt ctgccgcttt tcccaacgca tcgatctttg cgaccaggtt
     1441 gcgatgcagg tggaacggcg ccatccacag cttgctcaaa cgcggcaggc ggctctggct
     1501 ggctagatgc acaaacacat gctccatgtc ggtagtcagc gccgcatcgg ccgtgatgtg
     1561 gctcacatcg gtgtagagct tggcggtgcg ggggttgtag ttgccagtgg acagatgccc
     1621 atagcgttta agtaacttgc cctcacgtcg ggtgaccagc atcatcttgg cgtgggtttt
     1681 gaggcccacc acgccataca ccacctgcgc accgatagac tccagcatct cagcccaatt
     1741 gatgttggct tcctcatcaa agcgcgcctt gagctccacc accaccgtaa cctccttgcc
     1801 ctggcgcacc gcctcgcgca gcaggtccat caactccgag tcggcaccgg tacggtaaat
     1861 cgtctgtttg atagccagca cctgcgggtc atggacggct tcccgcagga aggtcaagac
     1921 accgtcaaaa ctttcgaacg gctggtgaat gaaaatgtcg cgctgcttga gctggtcaaa
     1981 gatggattgc cctgccacca gttgctgcgc aggagccgcc ttgtacgaag aaaagcacag
     2041 gtcggaccgg tctgccagat caatgagctg cgtcaggcgc accaaattga cagggccagg
     2101 cacacggaac aacgactcct gcggcagctc aaactgctcc agcagaaaat ccgacaggta
     2161 ttgcgagcac cccgcagaca cctccaggcg taccgcctgc ccaaagtgac gatgcaccag
     2221 cccctggcgt aatgcggtac gcaggttctt cacatcctct tcatccaccg atagatcgga
     2281 atggcgcgtc acccgaaact gtgaaaactg cccgaccgtt cggcccacaa acagctcacc
     2341 caggtgcgct cggatcacac tggaaatcga cacaaacaac gcccgcttgc ccgagacctt
     2401 ctccggcaga cggatgaacc gcggcaacac ccgtgtcact ttcacaatgg caatttcatt
     2461 ctcgcgaccg aatgcgtcct tgccacccag gcggacaatg aagttcaggg atttgttggc
     2521 cacctgcgga aacgggtgtg acggatccag ccccaccgga atgagcagcg ggcgcacttc
     2581 gcgctcaaag tactgcttca cccaagcccg ctgggcgctg ttgcgctcac cgtgcgaaat
     2641 gactttgatg ccctcggcct caaaagctgg caacagcttg tcgttgtaga tggcgtactg
     2701 tcgcgccacc agggattgca acgccgcagc caggcggttg aacgtctcga ccgtataggt
     2761 ccccttgcga tcgtgccgca tatgcgccac cagatggggt tcagcgcgca cctcaaagaa
     2821 ctcatcaagg ttggaggaca cgatacagag atagcgcagg cgctccagca aaggcacgtc
     2881 gtcgcgcaca gcccaactga gcacccgttc attgaatgcc aggatgctgt ggtcccggtc
     2941 cagaaggtcc ggcgccccct gttggggttg ctctttgctt ttaccgaaaa atgacaacat
     3001 ggccacacac ctgacaatga caaaacaatg aaggattgtt tacggctttg atgacatttg
     3061 tgtgacggtt tgtgcacata cgctggagtt tcctgtccgg gactggtttt gcgcaggcac
     3121 cagatgcccc acaaacagcc gggacgcatg ggcccaactg aaggtcaagg cccggctgcg
     3181 ggcctccacc cgcggcaccg ccagggcgtc agaccaggcc agcgacaggt ccgtattcag
     3241 agctccaccc tgcgcgggcg agcccaatac ctccaggggg ccgtcaaccg ggaaagccgc
     3301 caccggcgtc ccgctggcca tggcctccag catcaccagg ccaaaggttt ccgactggct
     3361 gggcatgaca aacacatcgg ctgctgcata cacactggcc agttctgcgc gcggaaggac
     3421 tcccagccag tagacaccgg aatagcgctc tttcaacgca gcctccagcg gccccacgcc
     3481 acacaccact ttggtcccgg ggacttgcag ctgcagaaag gcttcaatat tcttctcgta
     3541 cgagacccgg ccgacaaaca gcgacacagg gcgcgtcaga ttccccaggt gaacgcactc
     3601 cgttgcctgc ggatggtgac tgaacagccc catatccacg ccatgagtcc agccacgcag
     3661 atggcggaag ccacgcagat ggcggaagcc acgcagatgg cggaagccac gcgcctccag
     3721 taagcgcagc actccctgcg taggcaccag cacgccggag gacggcttgt ggaaatgtct
     3781 gaacacggca tacccgagcc acaagggcac cttcagtgcc gccttgagga tctccggaaa
     3841 acgcgtgtgg taagccgtgg tgaaggccag cttgcgccgc aggcaatagc gccgggccgc
     3901 ccagcccaac ggaccttcgg tggcaatgtg gatggcctcc ggggccagtg catccagctg
     3961 gcttgcgagc tgggcatagg gccgtaccgc cagatcaatc ccgtcatacc ccggacacgg
     4021 ccgggtgcgg aacagccccg gatggatcac cgtgacctga tggtccaacg catccatctc
     4081 acgcaccagt tccaccagtg tggtcaccac gccgttgacc tgcggctgcc aggcatcggt
     4141 aatcagggcc agtttcatgc gactaccccc attggctgcg gtgccgacgg ctcccggaat
     4201 tggctgctga gcacatcgtc ccagtagacc agttccagcc gcccgtccat gtgttccacc
     4261 agggcactgc ggctttcgac ccagtctccg tcgttgcaat acagcgtgcc gttgatggtg
     4321 cgcatttcgg cgcggtgaat atgcccacac accaccccgt cgtggccacg gctgcgcgcc
     4381 tctgctgcca cagccacttc gaaatcggtc acatagttca aggctttctt gaccttgtgc
     4441 ttgaggtaag ccgatagcga ccagtaaggc aagcccatgc gcgcccgcag gctgttgagg
     4501 tagcgattga gcttgagcgt gaattcgtag gcgttgtcac cgaggtacgc cagccacttg
     4561 gcgcactgga tcaccccatc gaaatagtca ccatgggtga tccagagcgt gcggccatcg
     4621 gccgtggtgt gcaccgtgtc ttcgcaaacc tccacgccgc caaagtggtg gcccacgaag
     4681 ccgcgcgcga attcatcgtg gttgccgggg ataaagacca cccgactgcc cttgcgggcg
     4741 ctgcgcagca gcttttgcac cacgtcgttg tgtgcctgcg gccagaacca gcggcggcgc
     4801 aactgccagc catccacaat atcgcccacc aggtacaggt agtcactggg gtggtgtttg
     4861 aggaattcca gcagggcttg agcctggcag cccgcggtac ccaggtggat gtccgatatg
     4921 aacaccgcgc ggtagcggtg ggttccgtgg gcgtggtcca cttcgtcttc ggcgcctggc
     4981 gccaaacctt gccagttggt gcccagtgcg tcgaaggtgg tacgcattta cgcccccaga
     5041 aagtgcttgc ggtagcggga aggcaaatcg gcaatgcgca tcagcatggg caagtcggcc
     5101 gaattgaaat ccgggtccca agccggagcg cccagc
//
LOCUS       DAOSJM010000158         5127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14514_length_5127_cov_3.290812, whole genome shotgun sequence.
ACCESSION   DAOSJM010000158 DAOSJM010000000
VERSION     DAOSJM010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14514_length_5127_cov_3.290812"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..377
                     /locus_tag="PKX82_06835"
     CDS             69..377
                     /locus_tag="PKX82_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6172 family protein"
                     /protein_id="HOF52005.1"
                     /translation="MKKTFSLAVEGKNPARLLDATKHEIRKYLKRERRRDLPEGVDFW
                     DFDCRFGPAQDTASVVHLAELIPMIDALVQNGGTQFYVEVLAKHGHRTPRTPTLDAED
                     "
     gene            391..996
                     /locus_tag="PKX82_06840"
     CDS             391..996
                     /locus_tag="PKX82_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOF52006.1"
                     /translation="MDITSLESLRSLYASARERSVKKELRQLDVHCTRFISLSPLVVI
                     SSSGASGALDASPRGGEPGFVRVQGHGTLLIPDAPGNNRLDTLENVVQTGQVGLLFFV
                     PGMDETLRVNGKAALSTLAAELELCKDAKRTPKLIIRVAVEAAYLHCAKALMRSALWD
                     ASRHVERSVMPSMGEMMKDQIGGDIPCETQEEMLARYAKDL"
     gene            1014..1625
                     /locus_tag="PKX82_06845"
     CDS             1014..1625
                     /locus_tag="PKX82_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HOF52007.1"
                     /translation="MASPDFSPSAERNKQPILDVLRTYLPEQGSALEIASGTGQHVVW
                     FAQNLPHWTWQPTDMHRGALYNIEVRANESDLANIHAAVELDVLAQPWPLQEETFDLV
                     LCINMLHIAPWASCAALMQGSARLLQPHGLLVTYGPYFEDGVPPTPSNVAFDQSLRAH
                     DPSWGIRRREDVEREANAAGLQMLARHEMPANNLMLVFRRAHG"
     gene            1635..2108
                     /locus_tag="PKX82_06850"
     CDS             1635..2108
                     /locus_tag="PKX82_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOF52008.1"
                     /translation="MNLTLSLFPLGWQHYFLGGLLVGTGTALLFVLTGRIGGMSTVFS
                     STWSYVVQRPFFQQARFVDSRNWRLVYAAGLILGAFIWWMGFAGGASQATAVPVWQLL
                     VGGFFVGYGARLGNGCTSGHGICGLGSMQLPSLMAVLTFMATAFLTANIAARWLA"
     gene            2105..2518
                     /locus_tag="PKX82_06855"
     CDS             2105..2518
                     /locus_tag="PKX82_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOF52009.1"
                     /translation="MSAARMLSTLAAGVLFGFGLSYATMIQPEVVLSFLRFQDFGLML
                     VMGGAVAVVLIAYKLAPRLMQHPVFDDHFHAHPSAWNRDTAKGAAIFGIGWGLCGVCP
                     GPAIAGLGTGNWDLLWALGGIALGALVQGLRATKP"
     gene            complement(2519..4084)
                     /locus_tag="PKX82_06860"
     CDS             complement(2519..4084)
                     /locus_tag="PKX82_06860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOF52010.1"
                     /translation="MLKYPAVVRRLSLRRDLMLLVLVCVLPVTFVSALVTYFSYLQQR
                     ESVEQQTELIARAVLADLEREIFTIESGLKVLATSNELSTGDLRAFHQRARDALRSGA
                     VHNYILTDPQGRQVVNTLLPYGTPLPTSGTPVALGRVFDDRATVLTDMFMAPVAKKLG
                     IAMGVPVGDADKVIFSLNVGVDPVRIQALISALALPEGWLVAVIDGSGTIIGRSRDAE
                     RFVGQKAVPEIVDVLAKGGQGRIESVTKEGVPVVTAYVTSASWKWGVVVGVPKNVLSR
                     DVMTQFASVLVAMLAVLGVGLWLARTISTRVLSSVRELNDAAISLGKGERVYLPAMQL
                     QEAEAVGKAIEQAALSMQKVKFLAQHDTLTELPNRLLFEEVAHHNLAFAQRRGQTLAV
                     LAVDLDGFKGVNDNLGHSAGDDVLRMVAQRIAHTIRGSDIVARMGGDEFLIMLTDVDR
                     ESAIETAERIVAVLSESYPGVELPVSASVGVALYPLCGSNLTELSAAADRALYRAKQE
                     GKRRAVLAEPGKS"
     gene            complement(4201..4584)
                     /locus_tag="PKX82_06865"
     CDS             complement(4201..4584)
                     /locus_tag="PKX82_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HOF52011.1"
                     /translation="MNYLLIKQLHITCAVISGSLFVLRGMWMLLRPELLQHRWVRVVP
                     HVVDTLLLGSALTMVALSGQFPFVLPWLTAKVVALLVYIVLGAVALKRGKTRAVRSLA
                     LVGAILVFGYIVAVALTRLVVPLSW"
     gene            4687..4914
                     /locus_tag="PKX82_06870"
     CDS             4687..4914
                     /locus_tag="PKX82_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HOF52012.1"
                     /translation="MTSVQEPLPPEAVKSALAALQDRATTLGLVLRTLPPEPTTCCGR
                     GCNGCVWEGYYAAVEFWREDAQSAIATASAH"
BASE COUNT         1061 a         1528 c         1504 g         1034 t
ORIGIN      
        1 ggtttctttg tcaggtgttg ggatgtgggc aggcataggg ccgtatcatc gccgctgaag
       61 gaatctccat gaaaaaaaca ttttctcttg ccgttgaagg caagaaccct gcgcgcctgc
      121 tggacgccac caaacacgaa atccgaaaat acctcaagcg tgagcgccgt cgcgacctgc
      181 ccgagggcgt ggatttctgg gattttgact gcagatttgg ccccgcgcag gacaccgcct
      241 cggtggtgca tctggccgag ttgataccca tgatcgatgc cttggtacaa aatggtggaa
      301 cccagttcta cgtggaagtt ctggcaaagc acggccaccg cactccacgc acccccaccc
      361 tggacgcaga agactgagag tttttttgcc atggacatca cttccctgga gtccctgcga
      421 tcactgtatg catctgcgcg tgagcgctcg gtaaagaagg agctccggca gctcgacgtg
      481 cactgcacac gctttatcag cttgtcaccg ctggtggtga tatccagctc cggcgccagt
      541 ggtgcgctgg acgcatcgcc gcgtggcggc gagcctggct ttgtcagagt gcaaggccat
      601 gggaccctgc tgattcccga tgcgccggga aacaaccgac tggatacgct ggaaaacgtt
      661 gtccagactg gtcaagtcgg tctgctgttt ttcgtgccag gcatggatga gaccctgcgc
      721 gtcaacggca aggccgctct atccacgcta gccgcagaac tggaactctg caaggatgca
      781 aagcgcaccc caaaattgat cattcgcgtg gcggtggaag cggcgtacct gcactgtgcc
      841 aaggcgctga tgcgctcggc actgtgggat gcatcacgcc atgtggaacg ttcggtgatg
      901 ccatcgatgg gcgagatgat gaaggaccag attggtggcg acattccttg cgagacacag
      961 gaagagatgc tggcgcgcta cgcgaaagat ctgtaaccac acgcgttgat cgtatggcgt
     1021 cacccgactt cagcccctct gccgaacgca acaaacagcc cattctagac gtgctgcgta
     1081 cctacctgcc agagcaaggc agcgcactgg agattgcctc cggcaccggc cagcatgtgg
     1141 tctggtttgc ccagaatctg ccgcattgga cctggcagcc cacggacatg caccgtggcg
     1201 cgctctacaa cattgaagtg cgcgccaatg agtcggacct tgccaacatc catgccgctg
     1261 tcgagctgga tgtgctggct caaccctggc cattgcagga agagacgttc gacctggtgc
     1321 tgtgcatcaa catgctgcac atcgcaccct gggccagttg cgccgcgctg atgcagggca
     1381 gcgcccggct tttgcagccg cacggcctgc tggtgaccta cggtccgtat tttgaagatg
     1441 gcgtaccccc aacgcccagc aatgtggcgt ttgaccagag tctgcgcgca cacgatccca
     1501 gctggggcat acggcgccgc gaggatgtgg aacgagaggc gaatgctgcc gggttacaga
     1561 tgctggcccg ccacgaaatg cccgcgaaca atctgatgct ggtgtttcgc cgcgcccacg
     1621 gataatctga cctcatgaat ctcacccttt ccctttttcc cctgggttgg cagcactatt
     1681 ttctgggcgg cctgctcgtt ggcaccggca ctgcccttct gtttgttctg actggccgca
     1741 tcggaggcat gagcacggtg ttttccagca cctggtctta cgtggtgcag cgcccctttt
     1801 tccagcaggc gcggtttgtg gattcacgca actggcggct ggtgtacgca gcagggctga
     1861 ttctgggtgc gtttatctgg tggatgggtt ttgcgggtgg agccagccag gcgacggccg
     1921 ttccggtatg gcaactgcta gttgggggat tcttcgtggg ctatggtgcc cggctgggaa
     1981 atggctgtac gtccggccac ggcatctgcg gcctgggttc aatgcaattg ccctcgctga
     2041 tggcggtgtt gaccttcatg gccaccgcgt ttctgaccgc caatatagcg gcaaggtggc
     2101 tggcatgagc gccgcacgga tgctatccac gctggcagcc ggcgtcctgt tcggctttgg
     2161 cctgtcctat gcgaccatga tccagcctga agtggttttg agctttctgc gctttcagga
     2221 ctttggcctg atgctggtga tgggtggagc cgtcgccgtg gtgttgatcg cctacaagct
     2281 ggctccccgt ctaatgcagc acccggtgtt cgacgaccac ttccacgcac accccagcgc
     2341 ctggaaccgc gatacggcca agggtgcagc cattttcggc atcggctggg gactgtgtgg
     2401 cgtgtgcccg ggtccggcca tagcaggcct gggcactggc aactgggacc tgctgtgggc
     2461 gttgggtggc atcgcactgg gtgcactcgt gcaggggctg cgggccacca agccctgatc
     2521 aactcttgcc cggctcggcc agtaccgctc gacgcttgcc ttcctgcttg gcgcggtaca
     2581 aggcccggtc tgcggcggcg ctcagttccg tcaggttgct tccgcacagt ggatacagag
     2641 caacacccac gctggccgaa accggcaact ccacgccggg atatgattca gatagcactg
     2701 cgacaatgcg ttcggcggtt tcgatggcac tttcgcggtc tacatcggtg agcatgatga
     2761 gaaactcatc accgcccatc cgggcaacaa tgtccgatcc gcggatagtg tgcgcgatgc
     2821 gctgggccac catacgcaga acatcgtccc ccgcactgtg tccgaggttg tcgttcacac
     2881 ccttgaatcc gtccaggtca accgccagaa cggcaagcgt ctggcccctg cgctgcgcaa
     2941 aggcaaggtt atggtgcgcc acttcttcaa acaagaggcg gttggggagt tcggtcaggg
     3001 tgtcgtgttg cgccagaaac ttgacttttt gcatggagag ggcagcctgc tcgatcgctt
     3061 tgcctacggc ctccgcctcc tgcagctgca tggcaggcaa gtacacccgc tcacctttgc
     3121 ccagggaaat ggcagcatca ttgagttcac gcactgaaga cagtacccgt gtagaaatag
     3181 tacgggccag ccacaagccc acccccagaa cagccagcat ggcaaccagc acactggcga
     3241 actgggtcat gacgtcacgt gaaagcacat tcttgggaac gccaacaaca acaccccact
     3301 tccatgatgc ggaagtcaca taggcggtaa ccacgggaac gccctccttg gtgaccgact
     3361 cgatgcgtcc ctgcccgcct ttcgccagta cgtccacgat ttcagggacc gctttttgtc
     3421 cgacaaagcg ctccgcatcg cgcgagcgcc cgatgatggt acccgagcca tcgataacgg
     3481 cgacgagcca tccctctgga agtgcaagcg cgctgatcag tgcctgaata cgtacaggat
     3541 ccacaccgac gttcagactg aagatcacct tatcagcatc accgacaggc acccccatcg
     3601 cgatacccag tttctttgca actggcgcca tgaacatgtc ggtaagaacg gttgcgcggt
     3661 catcgaacac acgacccaga gcgaccggcg taccactggt cggcagcggt gtgccatagg
     3721 gaagcagcgt gttgaccacc tggcgcccct gtgggtcggt aagaatgtag ttgtgcactg
     3781 caccggaccg aagcgcatcc cgggcacgct ggtgaaatgc ccgcaaatct ccggtagaaa
     3841 gttcgttcga agtcgccagc accttcaggc ctgactcgat ggtgaaaatt tcgcgctcta
     3901 gatccgcaag gacagcacgg gcgatcagtt ccgtctgctg ctcgacgctt tcacgctgct
     3961 gtaaatagga aaaatacgtt accaatgcac tgacgaaggt cacgggcagc acacagacaa
     4021 gcaccagcag catcaggtca cgacgcagcg acagacgacg gacaaccgcg gggtacttca
     4081 gcatggaggc ctgttccttg aagtgctgct gtggtctgca ctatcgcaga tggccgtgga
     4141 tggtgcaaca gacccgccgc cgacaaggtc ttccagatgc gtcctaggtg caaatgggac
     4201 ttaccaggac agcggcacca ccagccgcgt cagggccacc gcaacgatgt agccaaaaac
     4261 caggatggcg cccaccagcg ccaggctacg cacagcgcgc gtcttgccgc gtttgagcgc
     4321 gaccgccccc agcacgatat acaccagcaa agccacaacc ttggcagtaa gccatggcaa
     4381 cacaaagggg aattgcccac tcagggccac catggtcaac gcgctgccca gcagcagggt
     4441 atccaccaca tggggaacca cacggaccca gcggtgctgc agaagctctg gccttagaag
     4501 catccacatg ccgcgcaaca caaataagct gccgctgata acggcgcagg tgatgtgcag
     4561 ctgtttgatg agcagatagt tcatgtgcac tgttttcgtg atattttccc gattgtgctg
     4621 cagtttgcta tggtttccat agctatctgt gcaatgttga cgggggctac cccctaaaat
     4681 ctccagatga catcagtcca ggagccatta ccaccggaag cggtaaagtc cgcgttggcc
     4741 gctttgcaag acagagccac aacactgggc ctggtattgc ggacactgcc accggaaccc
     4801 accacctgct gcggtcgcgg atgcaacggt tgtgtctggg aaggctacta cgctgctgtg
     4861 gaattctggc gggaagacgc gcagtcagca attgcaacgg cgagcgcaca ctagctgttg
     4921 tctaccctca tattgcgata ggatgcgctg ggtaatcaaa caacaaaaag gcgttggagc
     4981 cgtgccggag attctgaatc tgtgtgcggc gatttggcag ttacgcgctg cggggcagac
     5041 acacaaggtc agcccgtcat gtgtcacata tgaaccctga tttctttgcg tctctggaaa
     5101 agatcatcgc agctgtgctg agcaagt
//
LOCUS       DAOSJM010000159         5085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14671_length_5085_cov_3.858847, whole genome shotgun sequence.
ACCESSION   DAOSJM010000159 DAOSJM010000000
VERSION     DAOSJM010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14671_length_5085_cov_3.858847"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PKX82_06875"
     CDS             <1..482
                     /locus_tag="PKX82_06875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOF52013.1"
                     /translation="SAAKALRDAPGCQFEQLIDLCGVDYSEYPAGSLDGQRYAVVSHL
                     LSVSLNQRVRLKVFAPDDDMPMVASVTDIWNSANWFEREAFDLFGIVFDGHNDLRRIL
                     TDYGFIGHPFRKDFPVTGHVEMRFDAEQARVIYQPVTIETREITPRIVREDNYGGLQ"
     gene            495..1748
                     /locus_tag="PKX82_06880"
     CDS             495..1748
                     /locus_tag="PKX82_06880"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOF52014.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAETKTYIQALPYMDRLDYVSMMCNEHAYCLAIEKLLGIEVPIRAQYIRVMFS
                     EITRLLNHLMWLGSHGNDCGSSTILIYTFREREDLFDMYEAASGARMHAAYFRPGGVY
                     RDLPDTMPQFKASKVRNAKGVAELNQNRQGSLLDFIDDFSQRFPKCLEEYHTLLTENR
                     IWKQRTVNIGIVTPERALNMGFTGPMLRGSGIAWDLRKKQPYDAYDKLDFDIPVGKTG
                     DVYDRYLVRMEEMKQSNRIIKQCVDWLKANPGPVITDNHKVAAPSRESMKTNMEGLIH
                     HFKLFTEGFHVPEGEAYAAVEHPKGEFGIYIVSDGANKPYRLKIRPPGFAHLAGLNEM
                     AQGHMIADAVSIIGTMDIVFGEIDR"
     gene            1745..2236
                     /gene="nuoE"
                     /locus_tag="PKX82_06885"
     CDS             1745..2236
                     /gene="nuoE"
                     /locus_tag="PKX82_06885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOF52015.1"
                     /translation="MISDATKARFAKEVAKYPADQKQSAVMACLSIVQQELGHVSADS
                     ENVVADYLGMAPMAVHEVTTFYNMYNQRPVGKFKLNVCTNLPCQLRDGQKALHHLEKK
                     LGISMGETTPDGLFTLQQSECLGACADSPVLLVNDRSMCSFMSNDKLDQLVDGIRSAE
                     GKV"
     gene            2233..3585
                     /gene="nuoF"
                     /locus_tag="PKX82_06890"
     CDS             2233..3585
                     /gene="nuoF"
                     /locus_tag="PKX82_06890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOF52016.1"
                     /translation="MSAQDVINQFVAKGVETCFHDRHINPQIYAGLNGSNWSIKDYEA
                     RGGYQALRKILGKDGGEGFTQDQVIATVKESGLRGRGGAGFPTGLKWSFMPRQFPGQK
                     YLVCNSDEGEPGTCKDRDILQFNPHIVIEGMAIAAYAMGIGVGYNYIHGEIFGSYERF
                     EAALEEARAAGFLGNNIMGSNFSFQLHAAHGFGAYICGEETALLESLEGKKGQPRFKP
                     PFPASFGLYGKPTTINNTETFAAVPWIIRNGGPAYLECGKPNNGGTKIYSVSGDVERP
                     GNYEIPMGTPFSKLLELAGGVRAGRQLKAVIPGGSSSPVLPASVIMECTMDYDSIAKA
                     GSMLGSGAVIVMDDSRCMVKALQRLSYFYMHESCGQCTPCREGTGWLSRMVDRIETGH
                     GRPSDMDLLDNVAENIMGRTICALGDAAAMPVRAMIKHFRHEFVHHIEHKSCVVPAYV
                     "
     gene            3597..>5085
                     /gene="nuoG"
                     /locus_tag="PKX82_06895"
     CDS             3597..>5085
                     /gene="nuoG"
                     /locus_tag="PKX82_06895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOF52017.1"
                     /translation="MIEIELDGKKVDIVEGSMIMHAADKAGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKMPKPMPACATPVTQGMIVRTKSDKAIKAQKSVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGSTSRYEEEKRVVFHKDVGPLISMEEMSRCIHCTRCVRFGQEI
                     AGAMELGMSHRGEHAEIETFVGATVDSELSGNMIDICPVGALTSKPFRYSARTWELSR
                     RKSVSPHDSTGANLIVQVKNNKVMRVVPLENDAVNECWIADRDRFSYEALNGEDRLTT
                     PLLKQGGVWKSVDWQTALEYVANGLKQIKANHGANAVGALVSPHSTLEELHLAGSLVR
                     GLGSENIDYRLRNAEFVTAPGARYLGTSIASLSQLQRVLIVGSNLRKDHPLFALRIRH
                     ATKLGCAVNVLNERASDWALPVANAMTVASSQWLQALADIAAAVAAEKGVAAPVGGNA
                     TPEAQAIAKSLLGGERKAVLLGNAAAHHAQASGLLGLANWIAAQTG"
BASE COUNT         1091 a         1377 c         1509 g         1108 t
ORIGIN      
        1 tgtcagccgc gaaagctctg cgtgatgcgc cgggctgcca gttcgagcag ctgatcgacc
       61 tgtgcggtgt ggactactct gagtatcctg cgggttctct ggatgggcag cgctacgcag
      121 tggtgagcca tttgctgtcg gtgagtctga atcaacgtgt gcgtctcaaa gtgtttgctc
      181 ccgatgacga tatgcccatg gtggcgtcgg ttacagacat ctggaattct gcgaactggt
      241 tcgagcgcga agcgtttgac ctgtttggca tcgtgttcga tgggcacaat gatttgcgcc
      301 gcattctgac ggactatggt tttatcggcc atcctttccg caaagacttt ccggtcaccg
      361 gacatgtcga aatgcgtttc gatgccgagc aggcgcgcgt tatttaccag ccggtcacga
      421 tcgaaacgcg ggaaatcacg ccgcgtatcg tccgtgaaga caattacggg ggcctgcaat
      481 aagcagaggt atgcatggct gaaatcaaga actacaccct gaactttgga ccgcagcacc
      541 cggcggcaca cggcgttttg cgtctggtgc tcgagctcga tggcgaggtg atccagcgtg
      601 ccgatccgca cattggattg ctgcaccgcg caacggaaaa gctggctgag accaagacct
      661 atatccaggc gctgccctat atggaccgtc tcgattacgt gtccatgatg tgcaatgagc
      721 acgcctattg cctggcaatc gagaagcttc tgggcatcga agtgccaatc cgggcccagt
      781 acatccgcgt gatgttctcc gaaattaccc gtctgctcaa ccacctgatg tggctgggtt
      841 cgcatggcaa tgattgcggc agttccacca tcctgatcta caccttccgt gagcgggaag
      901 acctgtttga catgtacgaa gccgccagtg gtgcacgcat gcacgcggcg tactttcgtc
      961 ccggtggtgt gtaccgtgat ctgcctgaca ccatgcccca gttcaaggcc agcaaggtgc
     1021 gcaatgccaa gggtgtggct gagctcaacc agaatcgcca gggttcgctg ctggacttca
     1081 ttgacgactt ttcccagcgc ttccccaagt gcctggagga ataccacacc ctgctgaccg
     1141 aaaaccgcat ctggaagcag cgcacggtca acatcggcat cgtgactccc gagcgtgcac
     1201 tcaatatggg ctttaccggt ccgatgttgc gcggctcagg tattgcctgg gatctgcgca
     1261 agaagcagcc ctacgatgcc tacgataagc tggattttga tattcccgtg ggtaaaactg
     1321 gcgacgtgta cgaccgctat ctggtgcgca tggaagagat gaagcagtcc aaccgcatca
     1381 tcaagcagtg cgtggactgg ctcaaggcca atcctggccc cgtcattacc gacaaccaca
     1441 aggtggctgc accttcgcgc gagtccatga agaccaatat ggaaggcttg attcaccatt
     1501 tcaagctgtt cactgaaggt ttccatgtgc cggaaggcga ggcctatgcg gctgtggagc
     1561 atccgaaggg tgaatttggt atttacatcg tcagtgatgg tgccaataaa ccgtatcgcc
     1621 tgaagatccg tccgcctggc tttgcgcatc tggcgggcct caacgagatg gctcaagggc
     1681 acatgatcgc cgacgcggtg tccatcattg gcaccatgga cattgttttt ggagagattg
     1741 atcgatgatc tccgacgcta ccaaagcccg cttcgccaaa gaagtggcca aataccccgc
     1801 tgaccagaag cagtctgctg tgatggcatg cctgtccatc gtccagcagg agctcgggca
     1861 cgttagtgct gacagcgaga atgtcgttgc cgattacctc ggcatggcgc ccatggctgt
     1921 gcatgaggtc acgaccttct acaacatgta caaccagcgg ccggtgggca agttcaagct
     1981 caacgtgtgt acgaacctgc cgtgccaact gcgtgatggg caaaaggctc ttcaccatct
     2041 ggagaagaag ctcggtatct cgatgggtga aacgacaccg gacggtttgt ttaccctgca
     2101 gcaaagcgaa tgccttggcg cgtgtgctga ctcgcctgtt ctgctggtca acgaccgctc
     2161 gatgtgcagt ttcatgagca acgacaagct ggaccagttg gtggacggta tccgttccgc
     2221 ggagggcaag gtatgagcgc gcaggacgtt atcaaccagt ttgttgccaa gggcgtggaa
     2281 acctgtttcc atgaccgcca tatcaatccg cagatatatg caggactgaa tggttcgaac
     2341 tggagcatca aggactacga agcccgtggt gggtaccagg ctctgcgcaa aattctgggc
     2401 aaagatggag gcgaaggctt cacccaggac caggtgattg ctacggtcaa agagtccggt
     2461 ttgcgcggac gcggcggtgc aggctttccg accggtctga agtggagctt tatgccccgc
     2521 cagttccccg gtcagaagta tctggtgtgc aattccgacg agggcgagcc cggtacctgc
     2581 aaagaccgcg acattctgca gttcaatccg cacatcgtga tcgaaggcat ggccattgct
     2641 gcgtatgcca tgggcatcgg cgtgggctac aactacatcc acggcgaaat tttcggttcc
     2701 tacgagcgct ttgaggcagc actggaagaa gcccgcgcgg ccggctttct gggtaacaac
     2761 atcatgggct cgaatttcag ctttcagctg catgccgccc acgggtttgg cgcctacatc
     2821 tgcggtgagg aaaccgcctt gttggagtcg ctcgaaggaa aaaaagggca gccccgcttc
     2881 aagccaccct ttcctgccag tttcggtctg tacggcaagc ccaccaccat caacaacacg
     2941 gaaaccttcg cagcggttcc ctggatcatt cgcaacgggg ggccggccta cctggagtgc
     3001 ggcaagccca ataacggtgg caccaagatt tactcggtga gcggtgacgt ggagcgtccc
     3061 ggaaactacg aaatcccgat gggaaccccg ttttccaaac tgctggaact ggcaggtggc
     3121 gtgcgtgcag gccgccagct caaggctgtg attcctggtg gttcatcgtc tccggtgttg
     3181 cctgcgtccg tcatcatgga atgcacgatg gattacgact ccattgccaa ggcaggctcc
     3241 atgctgggct ccggcgcggt cattgtgatg gacgattcgc gttgcatggt caaggcactg
     3301 cagcgtctga gctactttta catgcatgag tcctgcggac agtgcacgcc ctgccgtgaa
     3361 ggtacaggct ggttgtcacg catggttgac cgcattgaga ctggacacgg gcgtccgagc
     3421 gatatggatc tgctggacaa cgtggccgaa aacatcatgg gacgcacgat ttgtgccctg
     3481 ggcgatgcgg cagcgatgcc agtacgcgcc atgatcaaac atttccggca cgagtttgtg
     3541 caccacattg agcacaagag ctgtgtggtt ccagcctacg tttgagcaat accaaaatga
     3601 ttgaaatcga actcgacggc aagaaggtag acattgttga aggcagcatg atcatgcatg
     3661 ccgccgacaa ggctggtacc tacattcccc acttctgtta ccacaagaaa ctcagcattg
     3721 ctgccaattg ccgcatgtgc ctggtggatg tggagaagat gcccaaaccc atgcctgcct
     3781 gtgccactcc agtgacgcaa ggcatgattg ttcgcaccaa gagcgataag gccatcaagg
     3841 cccagaagtc ggtcatggaa ttcctgctga tcaaccatcc gctggattgc cctatttgcg
     3901 atcagggtgg cgagtgccag ttgcaggatc tggccgtggg atatggtggc agtacttcac
     3961 gctatgaaga agaaaagcgt gtggtgttcc acaaggatgt gggcccgctg atctccatgg
     4021 aagagatgag ccgctgcatc cattgcacac gttgcgtgcg tttcggtcag gaaattgcgg
     4081 gcgccatgga gctggggatg tcccaccgtg gtgagcatgc cgagattgaa accttcgttg
     4141 gtgcgacggt ggattccgag ttgtcgggca acatgattga catctgcccg gttggtgcat
     4201 tgaccagcaa gcctttccgt tacagcgccc gtacctggga gctgtcacgt cgcaagtcgg
     4261 tcagtccgca tgactcgacg ggcgccaatc tgattgtgca ggtgaaaaac aacaaggtga
     4321 tgcgtgtcgt tccgttggag aacgatgcgg tgaatgaatg ctggattgcc gaccgtgacc
     4381 gtttctctta cgaagctctc aatggagagg atcgccttac aacgcccctg ctcaaacagg
     4441 gtggtgtgtg gaagagcgtg gactggcaga cagcgcttga gtatgttgcc aatggactga
     4501 aacaaatcaa ggccaaccac ggtgcgaatg cggtgggcgc tttggtgagc ccgcacagca
     4561 cactggaaga gttgcatttg gcgggctcct tggtgcgtgg cttgggtagc gagaacatcg
     4621 actaccgtct gcgtaacgca gaatttgtga ctgcaccggg tgcgcgctac ctgggaactt
     4681 ccatcgcttc cttgtcccag ttgcagcgtg tgctgattgt gggttccaac ctccgcaagg
     4741 accatcccct gtttgcgttg cggattcgtc atgcgaccaa actgggttgc gctgtgaatg
     4801 tgctcaatga gcgcgccagt gattgggctt tgcccgtggc caatgccatg acggtggcgt
     4861 cgtcgcagtg gcttcaggca cttgccgata tcgccgctgc agttgcagcc gaaaaaggtg
     4921 tcgcagcacc ggtcggcgga aatgcaaccc ctgaagcgca agccattgcc aagtcgttgc
     4981 tcggtggtga acgcaaggca gtgctgttag gcaatgcagc agcacaccat gcccaggcct
     5041 ccggtttgct ggggcttgcg aactggatcg cagcgcagac cggcg
//
LOCUS       DAOSJM010000160         5050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14810_length_5050_cov_5.412813, whole genome shotgun sequence.
ACCESSION   DAOSJM010000160 DAOSJM010000000
VERSION     DAOSJM010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14810_length_5050_cov_5.412813"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PKX82_06900"
     CDS             complement(<1..1196)
                     /locus_tag="PKX82_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOF52018.1"
                     /translation="MTEKQDYRSTLNLPDTPFPMRGDLPKREPGWVKTWNEEGLYRRL
                     RDSRAGAPLFVLHDGPPYANGKLHIGHALNKVLKDMIVKSKQLAGFDAQYIPGWDCHG
                     LPIENAIEKLHGRNLSRDDMQAKSRAFATEQINQQREDFKRLGVLGDWERPYRTMDPA
                     NEAGEIRAFKRVIERGFVYRGLKPVYWCFDCGSSLAEFEIEYADKKSDTLDVAFEAAD
                     KQALLQAFLPGKLSDHPSAPLVAARLEAKPAFIVIWTTTAWTIPANQALNAHPELTYA
                     LVDTPRGLLVLADTLVEKCLERYGTTGTVIATTSGKQLGGLNFKHPLHDVHPGYARLS
                     PVYLADYVSDSDGTGIVHSAPAYGVDDFNSCVAHGLKYDDILNPVQGNGSYAEDLPLF
                     GGMNIW"
     gene            complement(1328..2302)
                     /locus_tag="PKX82_06905"
     CDS             complement(1328..2302)
                     /locus_tag="PKX82_06905"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOF52019.1"
                     /translation="MKVFRGFHHPEVASACAVTIGNFDGVHRGHQAMLALLRGEAQQR
                     GVPSCVLTFEPHPRDYFAGLYKKPELAPARVGTLRDKLVDLAHCGVDQAVVLPFDARL
                     AAQTPQAFVDQVLLQGLGARYVLVGDDFRFGAQRAGDYTFLDNAGQTQGFDVARMNSY
                     EVHGLRVSSSAVRDALGEGRMQDTARLLGRPYCISGHVVHGRKLGRTLGFKTLNLRFS
                     HWKPAASGIFAVLVHGLSEQPIQGVANLGVRPSLDPTDVNGGRVLLETHCLDWPAHLG
                     PEGAYGKIIRVELLHKLHDELKYDGLESLTKGIRQDCDDARAWFAARI"
     gene            2481..3023
                     /locus_tag="PKX82_06910"
     CDS             2481..3023
                     /locus_tag="PKX82_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOF52020.1"
                     /translation="MKVLKLSAQGLPQSWISLEQAVIHYAAGEVRWESGGEVAIFRGG
                     HNAITGQQSIIRVNSIIGTRGVPNINPFNLRPGLTNAKLFIRDRNVCAYCGGHFHETD
                     LTREHIIPFAQNGIDTWMNVVTACKLSPLFLLLHRRVIDNSRLRYRNGCQVATRKYCR
                     FGLRVHPKSSSVTPTERAES"
     gene            3020..3580
                     /locus_tag="PKX82_06915"
     CDS             3020..3580
                     /locus_tag="PKX82_06915"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOF52021.1"
                     /translation="MNELFLAIDSTGYPSGWLSWQEAVTNEVLGKVSYGFGDYEFTFT
                     GGKNRDSGLNSTVTLKSILVMHGRNRIASKYATIPLSNQALFRRDRFTCAYCGEIHMR
                     GLTRDHIVPLSRGGIDAWHNVCACCSDCNRRKNANLLEELGWELLFLPYAPNHQEGLL
                     LQNRRILCDQMELLKSMLPKHSRLAM"
     gene            3838..>5050
                     /locus_tag="PKX82_06920"
     CDS             3838..>5050
                     /locus_tag="PKX82_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010794468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOF52022.1"
                     /translation="MTVTNELIDSLLADYKKPEDLIGENGLLKLLTKKLVERALQAEM
                     AEHLGHAKNDTVANPAGNTRNGKSKKTLKGDFGELPIEIPRDRHGSFEPQIVTKHQTR
                     WAGFDEKILSLYARGMTVREIQSHLEEMYGAEVSPTLISSVTDAVMDEAKAWQARPLD
                     ALYPIVYLDCIHVKTRDAGAVRAKAVYLALGISMEGEKEILGLWIAQTEGAKFWLQVV
                     TELKNRGVQDIFIACVDGLKGFPDAIEAVYPKTAVQLCLVHMVRHSLNYVSWKLRKAV
                     AADLKAIYAASTVDEAAIRLNEFDDKWGTDYPTIVKSWRSNWARITPFFDYPPEIRRI
                     IYTTNAIESVNMSLRKITKNRGSFPSDDALLKLFYLALANISKKWTMPLRDWKAALTR
                     FTIQFEERMPKT"
BASE COUNT         1039 a         1410 c         1543 g         1058 t
ORIGIN      
        1 ttccagatgt tcatgccacc aaacaggggc aggtcttcgg catagctgcc gttaccctgc
       61 accgggttga ggatgtcgtc gtacttcagg ccatgggcca cgcagctgtt gaagtcgtcc
      121 acgccatagg caggggccga gtgcacgatg ccggtgccgt cggaatcact cacatagtcg
      181 gccaggtaaa ccggcgacag gcgggcatag cccgggtgca cgtcgtgtaa cgggtgcttg
      241 aagttcaggc caccgagctg tttgccgctg gtggtggcaa tcaccgtgcc ggtggtgcca
      301 tagcgttcca ggcatttttc gaccagcgtg tcagccagca ccagcagtcc acggggtgta
      361 tccaccagcg cgtaggtcag ctcggggtgg gcgttgaggg cctgattggc agggatggtc
      421 caggcggtgg tagtccagat gacgatgaag gcgggcttgg cttccagccg cgcagccacc
      481 agcggtgcgg aggggtggtc cgacagcttg ccaggcagga aggcctgcag cagcgcctgc
      541 ttgtcggccg cttcaaaagc cacgtccagc gtgtcgctct ttttgtcggc gtattcgatt
      601 tcgaattcgg ccaacgacga gccgcagtca aagcaccagt acacgggctt cagaccgcgg
      661 tagacaaagc cgcgttcgat cacgcgtttg aaggcgcgga tttcgccggc ttcattggcc
      721 gggtccatgg tgcggtacgg gcgctcccaa tcacccagta cgcccaggcg cttgaagtct
      781 tcgcgctgct ggttgatctg ttcggtggca aatgcgcggc tcttggcctg catgtcgtca
      841 cggctcagat tgcgcccgtg cagtttttcg atggcattct cgatcggtag accgtggcag
      901 tcccagccgg ggatgtactg cgcatcaaag cccgcgagct gcttggactt gacgatcatg
      961 tccttgagca ccttgttcag cgcgtggccg atgtgcagct tgccgttggc gtagggcggg
     1021 ccgtcgtgca gcacaaacag gggagcacca gcacggctat cgcgcaggcg gcggtacagg
     1081 ccttcctcgt tccaggtttt tacccatcct ggctcgcgct tgggcagatc gccgcgcatc
     1141 gggaaggggg tgtcaggcag gttcagggta ctgcggtaat cttgcttttc agtcatggcg
     1201 agttcaatcc aggtaatcgg gtgggggccc ctagtgcact gcgggaggtg ctaatggcgg
     1261 ggtgactctc tgtgagggca gaagtgacgc ggggcaaggt gcgtcgctga ccgggtggtc
     1321 ggccccgtca aattcgggcc gcgaaccagg cccgagcgtc atcgcagtcc tggcgtatgc
     1381 cctttgtcag ggattccaga ccgtcgtact tcagctcgtc gtgcagtttg tgcagcagtt
     1441 ccacgcggat gattttaccg taggccccct cgggacccag atgggcgggc cagtccaggc
     1501 aatgggtttc cagcaatacg cggcccccgt tgacatcggt cgggtccagc gacgggcgta
     1561 ccccaaggtt ggccacgccc tggatcggct gctcggacag gccatgcacc agtacggcga
     1621 agatgccgct ggcagcgggc ttccagtggg aaaagcgcaa gttcagcgtc ttgaacccca
     1681 gtgtgcggcc cagcttgcgc ccgtgcacca cgtggccgga gatgcaatac ggacgcccca
     1741 gcaggcgggc ggtgtcctgc atgcggcctt cacccagcgc atcgcgcaca gcggagctgg
     1801 agacacgcag gccatggact tcgtaactgt tcatgcgggc cacgtcgaag ccctgcgttt
     1861 ggccagcgtt gtccagaaaa gtgtagtcac ccgcgcgctg tgccccaaaa cggaaatcat
     1921 caccgaccag cacatagcgg gcgcccagtc cctgcagcag cacctggtcg acgaaggcct
     1981 ggggcgtctg tgccgccaga cgggcatcaa agggcagcac cacggcctgg tcgaccccgc
     2041 aatgggccag gtccaccagc ttgtcgcgca gcgtacccac gcgggctggt gcaagctccg
     2101 gcttcttgta caggccggca aagtagtcgc gcggatgcgg ctcgaaggtc aatacgcaac
     2161 tcggaacgcc gcgttgctgg gcttccccgc gcagcagcgc cagcatggcc tggtggcccc
     2221 ggtggacgcc gtcgaagtta ccaatggtta ccgcgcaggc gctggcaact tcagggtggt
     2281 ggaaaccgcg gaaaaccttc atggatttgt tatctttttg atagcaggct gcgcacaccg
     2341 cacgagggcc cgacgtcaat ttgtcatgga aacactgtat attgtgacgc agagcgggga
     2401 ttgggcgtag tccggggccg cgcttgcaga catttgcagt ccgtgttggt ggtagttttt
     2461 accgttgatg gaggcgttgc gtgaaagtct tgaagttgtc ggcccagggt ttgccccagt
     2521 cgtggatctc gctggaacag gcggtcatcc actacgcggc gggagaggtg cgctgggaat
     2581 cggggggcga ggttgccata ttccggggcg ggcataacgc catcaccgga cagcagtcca
     2641 tcatccgggt caacagcatc atcggcaccc gtggcgtgcc caacatcaac cccttcaacc
     2701 tgcgacccgg cctgaccaac gcaaagttgt tcatccgcga ccgcaatgtg tgcgcctact
     2761 gcggcgggca ctttcatgag actgacctga cgcgtgagca catcatcccg ttcgcccaga
     2821 acggcatcga tacctggatg aacgtggtca cggcctgcaa gttatccccc ctgtttttat
     2881 tactacatcg tcgtgtgatt gacaactccc gtctgagata tcgaaatggg tgtcaagttg
     2941 caactcgtaa atattgccgg tttggtttac gagttcatcc caaaagctct tcggtcactc
     3001 cgacagagcg agctgagtca tgaacgagct ttttcttgca attgatagca ctgggtatcc
     3061 ttcgggttgg ctctcttggc aagaagccgt caccaacgag gtgcttggta aggtgtcata
     3121 tggttttggc gactatgagt tcacttttac cggtggcaag aatcgggatt ctgggttgaa
     3181 ctccacggtt acgctgaaga gcatcttggt aatgcatggt cgcaatcgca tcgcttcgaa
     3241 gtacgcgacc ataccgttgt cgaatcaggc gctctttagg agagatcgct ttacgtgcgc
     3301 ctattgcggc gaaatacaca tgcgaggttt aacccgagat cacatcgttc cgctttcgcg
     3361 cggcggaatt gatgcgtggc ataacgtttg tgcctgttgc tctgactgca atcgtcgtaa
     3421 aaatgcaaat ttgctcgaag agttgggttg ggagttgttg ttcctaccat atgcaccaaa
     3481 ccaccaagaa ggcttgctcc ttcagaatcg acgcattctt tgtgaccaaa tggaattgtt
     3541 gaaatctatg cttccaaagc actctcgatt ggcgatgtag tcgctttcgg tacttgaggg
     3601 agtgcctgcg acaatattca atatccacat ttcattgtgg atgaagcggt cgatgtggct
     3661 tgacgtgaaa gacatacctc atcttgcgct gtaactccca cttcgtgggg cagttcgcta
     3721 ccacgtgagc gtttgttgct aacaagccga ggagcagaca gaaacggggg tgtaagaact
     3781 tttgtgtaaa cggtcaattg gcaggaaact gctgcactgc aatggaaatg caaataaatg
     3841 accgtcacga acgaattgat cgacagcctg ctggctgatt acaagaaacc tgaagacctc
     3901 atcggtgaga acggcctgct caagctgctc accaagaaac tggtggagcg tgccctgcaa
     3961 gccgagatgg ccgaacactt gggccacgcc aagaacgaca cggttgcaaa ccctgctggt
     4021 aacacccgca acggcaagag caaaaagacg ctcaaaggcg acttcggcga gttgcccata
     4081 gaaatcccgc gggatcggca tggcagcttc gagccgcaga tcgtgaccaa acaccaaacc
     4141 cgctgggcag gctttgacga gaagatcctg tctttgtacg cccgtggcat gacggtgcgc
     4201 gagatccaga gccatctgga agagatgtat ggcgcagaag tttcgcccac gctgatctcc
     4261 tcggtcacgg acgcggtgat ggacgaagcc aaagcctggc aggcccgtcc gctggatgcg
     4321 ctgtacccca tcgtctactt ggactgcatc catgtcaaaa cccgtgacgc tggtgcagtg
     4381 cgtgccaaag ctgtttacct ggcgctgggc atcagcatgg agggcgagaa ggaaattcta
     4441 ggcctgtgga tcgcccagac tgagggtgcc aagttctggc tgcaggttgt cacggaactc
     4501 aagaaccggg gcgtgcagga catcttcatt gcctgcgtgg atggcttaaa gggcttcccc
     4561 gatgccatcg aggcggtgta ccccaagacg gcggtgcagc tttgcttggt gcacatggtg
     4621 cgccacagtc tgaactatgt gagctggaaa ctgcgcaagg cggtggctgc tgacttgaaa
     4681 gccatctatg ccgcctcgac cgtggacgag gcagccatca ggctcaacga attcgatgac
     4741 aaatggggca ctgactatcc gaccatcgtc aagtcctggc gcagcaactg ggcccgtatc
     4801 acaccgttct tcgactaccc gccggagata cggcgcatca tttacacgac caacgcgatt
     4861 gagtcggtga acatgagttt gcgcaagatc accaagaacc ggggctcatt ccccagtgac
     4921 gacgcgctgt tgaagttgtt ctatcttgcc ttggccaaca tcagcaagaa atggactatg
     4981 ccgctgcgag actggaaagc tgcattgacc cggtttacca tccagtttga agagaggatg
     5041 cccaagactt
//
LOCUS       DAOSJM010000161         5039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14847_length_5039_cov_4.086477, whole genome shotgun sequence.
ACCESSION   DAOSJM010000161 DAOSJM010000000
VERSION     DAOSJM010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14847_length_5039_cov_4.086477"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="PKX82_06925"
     CDS             <1..896
                     /locus_tag="PKX82_06925"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOF52023.1"
                     /translation="AKVMSEGFLQAQREMYAKQAREADIIITTALIPGKPAPRLITAE
                     MVQSMKPGSVIVDMAAERGGNCELTEPGQAVVKHGVTIVGYTDLASRLSKQSSTLYAT
                     NLFRLAEELCKTKDGVANVNMEDDAIRGLTVIKDGQITWPAPASKLPAAPAAAAKATP
                     VAVKKPAHGHGAASEPMSAKSLAILMAVAALLFWVIGSYAPPAFLGHFTVFVLACFVG
                     YMVVWNVKPALHTPLMSVTNAISSIIAIGALVQVSPVAGAVGRPDSLITWLAAAAIVL
                     TAINMFGGFAVTQRMLAMFRK"
     gene            909..2321
                     /gene="pntB"
                     /locus_tag="PKX82_06930"
     CDS             909..2321
                     /gene="pntB"
                     /locus_tag="PKX82_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HOF52024.1"
                     /translation="MSASLATVSYIGATILFILSLGGLSNQSTALRGNLYGMVGMALA
                     VLATVFGPTVTAAGLPWIAGAMVLGGGVGLYAARTVQMTQMPELVALMHSMVGLAACL
                     VGFASYVDTSTQHTGAEKVIHEVEIYIGILIGAVTFSGSLIAFGKLNGKIGGKPLLLP
                     GRHLLNLAGLLVVVWFGREFIQSTSVAEGMTPLIVMTVVALLFGVHMVMAIGGADMPV
                     VVSMLNSYSGWAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCNAMNRSFISVIAGG
                     FGTTSATPTAAAGAAQPAGEVVAVNAGETAELLREAKSVIIVPGYGMAVAQAQHTVFE
                     ITRTLREKGVNVRFGIHPVAGRMPGHMNVLLAEAKVPYDIVFEMEEINDDFPDTDVVM
                     VIGANDIVNPSAQDDPTSPIAGMPVLEVWKAKTSIVMKRSMASGYAGVDNPLFYKDNN
                     RMLFGDAKKMLDEVLGSLKG"
     gene            2412..4085
                     /locus_tag="PKX82_06935"
     CDS             2412..4085
                     /locus_tag="PKX82_06935"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOF52025.1"
                     /translation="MTERVWLKSYPPGVPSDIDTSQYSSLVDLVEESFSKHASKVAYS
                     FMGKEVTYAQTDSLSQAFAAYLQSLGLVKGDRVAIMMLNMPQYPVAVAGILRAGMVVV
                     NVNPLYTPRELEHQLKDSGAKAIVVIETFATTLEKCIAATPVKHVVLCSMGDQLGFLK
                     GALVNYVVRNVKKMVPAFNLPGAVRFNAAIALGTKAPFKKQVSKSDDVAVLQYTGGTT
                     GVSKGAVLLHRNVIANVLQSEAWNEPVMKKVPANEQPTSVCAIPLYHIFAFTVGMMLS
                     MRTGGKMILIANPRDIPGVLKELAKHKIHSFPAVNTLFNGLANHPDFTKVDWSHLKVS
                     VGGGSAVQSSVAKLWFEKTGCPICEGYGLSETSPSASCNLVTATAYTGTIGVPLPSTY
                     MKLLDDDGHEVPAGQPGEIAIKGPQVMAGYWQRPDETAKVMTSDGYFKTGDVGVVDEK
                     GYFKIVDRKKDMILVSGFNVFPTELEDVVAQIPGVLECACVGVPDAKTGEAVKLVLVK
                     KDPNLTEADVRAFCRENLTGYKQPKVIEFRGELPKTPVGKILRRELREK"
     gene            4082..4828
                     /locus_tag="PKX82_06940"
     CDS             4082..4828
                     /locus_tag="PKX82_06940"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOF52026.1"
                     /translation="MTVAAPLAILSALGEEQQGLMGELTEAQCIPYAGREFWLGRLHG
                     QPVVLALSRIGKVAAATTASALIERFGASRVVFTGVAGGVGDGVHVGDVVVGTGFVQH
                     DMDASPLFPRYQVPLTGRSVFDGDAPLTAQLLQAAQVALADGGPLGKACVHQGLIASG
                     DRFVNGREESAVLVSSLRAAGYAPLAVEMEGAAVAQVCADHGVPFAAVRTISDRADDT
                     AHVDFPQFVKDVASLYALRIVTVLVYSLSK"
     gene            complement(4879..>5039)
                     /locus_tag="PKX82_06945"
     CDS             complement(4879..>5039)
                     /locus_tag="PKX82_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOF52027.1"
                     /translation="LLPPTLIASLYGMNFKYMPELDWSLGYPYALALMVASALVPMWY
                     FRKRGWLK"
BASE COUNT          965 a         1447 c         1617 g         1010 t
ORIGIN      
        1 acgccaaggt gatgagcgaa ggtttcctgc aggcgcagcg cgagatgtac gccaagcagg
       61 cccgcgaggc cgacatcatc atcaccaccg cgctgatccc cggcaagccc gcgcccaggc
      121 tgatcaccgc cgagatggtg cagagcatga agcccggcag cgtgatcgtc gacatggcgg
      181 ccgagcgggg cggcaactgc gagctgaccg aaccgggcca ggccgtggtg aaacacggtg
      241 tgaccattgt gggttacacc gacctggcct cgcgcctgtc caagcagtcc tcgaccctgt
      301 acgcgaccaa cctgtttcgc cttgccgagg agttgtgcaa gaccaaggac ggcgtcgcca
      361 acgtcaacat ggaagacgac gccatccgtg gcctgaccgt catcaaggac ggccagatca
      421 cctggcccgc accagcgtcc aagctgcctg cggcccccgc cgctgcggcc aaggcgactc
      481 cggtcgcggt gaaaaagccg gcccatggcc acggcgctgc gagcgaaccc atgtcggcca
      541 agagcctggc catcctgatg gccgtggctg cgctgctgtt ctgggtgatc ggcagctacg
      601 cgccgcccgc tttcctgggg cacttcacgg tgtttgtgct ggcctgtttt gtgggctaca
      661 tggtggtgtg gaacgttaaa cccgcgctgc acacgccgct catgagcgtg accaatgcca
      721 tcagcagcat catcgccatc ggcgcgctgg tgcaggtctc gccggtggcg ggtgcggtgg
      781 ggcgtcctga cagcctgatc acctggctcg ccgccgcagc gattgtgctg acggccatca
      841 atatgtttgg cggctttgcc gtcacccagc gcatgctggc catgttccgc aagtaaggag
      901 acctgaagat gtctgcaagt ttggctacgg tctcctacat tggcgcgacc attctgttta
      961 ttctgagcct gggggggctg tccaaccagt ccaccgcgct gcgcggcaat ctgtacggca
     1021 tggtgggcat ggcgctggcg gtgctggcca cggtgtttgg cccgacggtc accgcagctg
     1081 ggctgccctg gattgccggg gccatggtgc tggggggtgg cgtgggccta tatgccgccc
     1141 gcacggtgca aatgacccag atgcccgaat tggtggcact gatgcacagc atggtgggtc
     1201 tggctgcctg cctggttggt tttgccagct atgtggacac ctccacccag cacacgggcg
     1261 ccgaaaaggt gattcatgag gtggagattt acatcggcat cctgattggt gcggtcactt
     1321 tttccggctc gctgattgcc ttcggcaagc tcaacggcaa gattggcggc aagcccttgc
     1381 tgctgccggg ccgccacctg ttgaatctgg cgggcctgct ggtggtggtc tggtttgggc
     1441 gtgagttcat ccagtcgacc tcggtggccg agggcatgac cccgctgatc gtgatgaccg
     1501 tggtggcctt gctgtttggc gtgcacatgg tgatggccat tggcggcgcc gacatgcccg
     1561 tggtggtgtc catgctcaac agctactccg gctgggccgc ggcggccacc ggcttcatgc
     1621 tgtccaacga cctgctgatc gtgacgggtg ccttggttgg ctccagcggc gccatcttgt
     1681 cctacatcat gtgcaatgcc atgaaccgca gcttcatcag cgtgattgcg ggcggctttg
     1741 gcaccaccag cgccacaccc acggctgctg ccggcgcggc ccagcccgca ggtgaggtcg
     1801 tggccgtgaa cgcgggtgag accgccgagc tgctgcgcga ggccaagagt gtgatcattg
     1861 tgccgggcta cggcatggcc gtggctcagg ctcagcacac ggtattcgag attacgcgca
     1921 ccctgcgcga aaaaggcgtg aatgtgcgct ttggcatcca cccggtggct ggccgcatgc
     1981 cgggtcacat gaatgtgctg ctggccgagg ccaaggtgcc ctacgacatc gtgttcgaga
     2041 tggaagaaat caacgacgac ttccccgaca ccgacgtggt gatggtgatc ggtgccaacg
     2101 acatcgtgaa cccctccgcg caggacgacc ccaccagccc gattgccggc atgcccgtgc
     2161 tggaagtgtg gaaggcgaag acctccatcg tcatgaagcg cagcatggct tcaggttatg
     2221 ccggggtgga taatccgctg ttctacaagg acaacaaccg catgttgttc ggagatgcca
     2281 agaagatgct ggacgaggta ctgggcagtc tcaagggctg atttccttcg cgccctaggg
     2341 aaaacacact attccagcgt cctttcgacc ctgacaatat gcaccgcaca gagatgaatt
     2401 acggagaaga catgactgaa cgcgtttggc tcaaaagcta cccaccgggt gtgccttcgg
     2461 atattgatac cagccagtac agctcgctgg tcgatttggt ggaggagagt ttcagcaagc
     2521 acgccagcaa ggtggcctac agcttcatgg gcaaggaagt tacctatgcc caaaccgata
     2581 gcctgagcca ggcgttcgcg gcgtacctgc agagcctggg gttggtcaag ggtgaccggg
     2641 ttgccatcat gatgctgaac atgccccagt accctgtggc agtcgcgggt atcctgcgcg
     2701 cgggcatggt ggtggtcaac gtgaatccac tgtacacacc gcgtgaactg gagcatcagc
     2761 tcaaggactc gggagccaag gccattgtgg tcattgaaac ctttgcgacc acactggaaa
     2821 aatgcattgc cgcaacgccg gtcaagcacg ttgtgctgtg ttccatgggc gatcagttgg
     2881 gtttcctcaa gggggccctg gtcaactacg tggtgcgcaa cgtcaagaag atggtgccgg
     2941 cgttcaacct ccctggcgcc gtgcgcttca atgctgcaat tgccctgggc accaaggcgc
     3001 cgttcaaaaa acaggtcagc aagtccgacg atgtggcggt gctgcaatac acgggcggaa
     3061 ccacgggggt ttccaagggt gcggtattgc ttcaccgcaa tgtcattgcc aatgtgctgc
     3121 agtctgaggc ctggaacgag ccggtcatga agaaggtgcc ggccaatgag cagcccacca
     3181 gtgtgtgcgc cattccgctg taccacatct ttgcgtttac ggtgggcatg atgctgtcca
     3241 tgcgtacggg tggcaagatg attctgattg ccaatccgcg ggatattccc ggggtgctca
     3301 aggagttggc caagcacaag attcacagct tccccgccgt caacaccctg ttcaacggct
     3361 tggcaaacca tcctgatttc accaaggtgg actggagcca cctgaaggtc tcggtgggcg
     3421 gtggatccgc ggtgcagagc agcgtggcca agctgtggtt cgaaaagacc ggttgcccca
     3481 tttgtgaagg atatggactc agcgaaacct ctccttcggc cagctgcaac ctggtcactg
     3541 cgacggccta cacgggaacc atcggcgtac cgttgcccag cacctatatg aaactgctgg
     3601 acgatgacgg ccatgaagtt ccggctggcc agccaggaga aatcgccatc aagggacccc
     3661 aggtcatggc gggatactgg cagcgcccgg acgaaaccgc caaggtcatg acgtccgacg
     3721 ggtatttcaa gaccggtgac gtgggggttg tggacgagaa gggctacttc aagatcgtgg
     3781 accgcaagaa ggacatgatt ctggtgagcg gcttcaatgt gttccccacg gagctggaag
     3841 acgtcgttgc acagattcct ggcgtgctgg aatgcgcatg cgttggtgtg cctgatgcca
     3901 agaccggcga agccgtcaaa ttagtgctgg tcaaaaaaga ccccaacttg accgaagcgg
     3961 atgtgcgtgc tttctgccgc gaaaacctga cggggtacaa acagcccaaa gtcattgagt
     4021 tccgtggtga attgcccaag acccctgtgg gcaagattct gcgccgcgaa ctgcgggaaa
     4081 agtgaccgta gctgcgccgc tggccattct cagtgccctg ggcgaagagc aacagggact
     4141 gatgggcgaa ttgacggaag cgcaatgcat tccatatgcc gggcgggagt tctggttggg
     4201 gcgtttgcat ggccagccgg ttgtgctggc cttgtcccgc attggcaagg tggctgccgc
     4261 aaccacggca tcggcgctga ttgaacgttt cggcgccagc cgcgtagtct ttaccggcgt
     4321 ggcgggtggt gttggcgacg gcgtccacgt cggtgatgtg gtggtgggca caggctttgt
     4381 gcagcacgat atggatgcct caccattgtt tccgcgctac caggtgccac tgacaggccg
     4441 gtcggtattt gacggtgatg ccccattgac agcccaactc ctgcaggcgg cccaagtggc
     4501 cctggccgat ggcgggcctc tgggcaaggc ttgtgtgcac cagggattga ttgccagtgg
     4561 agatcggttt gtcaacggcc gggaggagtc ggccgtgctg gtgagttccc tgcgggcagc
     4621 tgggtatgcc ccgctggccg tggaaatgga aggcgccgct gtggcccagg tgtgtgccga
     4681 ccacggcgtc ccttttgctg cagtgcgcac catttcagac cgtgccgacg acacagccca
     4741 tgtcgatttt ccacagtttg taaaggatgt ggcgagtctc tacgctttgc ggatcgttac
     4801 ggttttggtc tattcgctat caaaataata gctagctgcg catatcctgt gcggggttgg
     4861 tgccaatttt cttcaaaatt acttgagcca gccccgtttg cggaagtacc acatgggcac
     4921 cagcgcgctg gcgaccatca gggccagggc atagggatag cccagagacc agtccagctc
     4981 cggcatgtac ttgaagttca tgccatacag gctggcaatc agggtgggcg gcagcaagg
//
LOCUS       DAOSJM010000162         5036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14857_length_5036_cov_3.893194, whole genome shotgun sequence.
ACCESSION   DAOSJM010000162 DAOSJM010000000
VERSION     DAOSJM010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14857_length_5036_cov_3.893194"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..834)
                     /locus_tag="PKX82_06950"
                     /pseudo
     CDS             complement(85..834)
                     /locus_tag="PKX82_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
     gene            897..2105
                     /locus_tag="PKX82_06955"
     CDS             897..2105
                     /locus_tag="PKX82_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF52028.1"
                     /translation="MGSGNYVIRVQTLPSQVDASAWDALLQKQSQPSPFMRHAYLAAL
                     HSSQSACAATGWAPHFITLERHGALEAACVVYLKNHSHGEYVFDSAWASAYRQHGVPY
                     YPKAVVAVPFTPVPGTRLLARDNAARTALVHALIAWCKQQAVSSLHLLFAAEEDLAAC
                     AGAGLMLRHTVQFHWTNTSPGYADFDAFLATLAQDKRKKIRQERRKVADAGVTFRQVQ
                     GSGITTEDWDFFYRCYERTYLEHGNPPYLTRDFFSRMESTMPENWLLFVAQRNGQNIA
                     SSLIAIDAHSTGARGQNGTESAKIAYGRYWGALESVDCLHFEACYYQPLQWCIAHGYQ
                     RFEGGAQGEHKMARALLPVRTSSAHWLAHPAFSDAVSHFLEREGAGVAKYLQDLEDRN
                     PFRQTGGAQS"
     gene            complement(2107..2610)
                     /locus_tag="PKX82_06960"
     CDS             complement(2107..2610)
                     /locus_tag="PKX82_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOF52029.1"
                     /translation="MEIMNSPSSAGGAASGGKPLADSGQYLTLRLGSEEYAIDILRVQ
                     EIRSYEEPTRMVNSPSFIKGVVNLRGVIVPIADLRLKLHVPDAQYNEFTVVIILNIHG
                     AVVGAVVDAVSDVVTLDSATIKPAPQFDSGIDSRFILGLVTLGERTLIVMNVEALLSN
                     AELGIAR"
     gene            2825..3619
                     /locus_tag="PKX82_06965"
     CDS             2825..3619
                     /locus_tag="PKX82_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOF52030.1"
                     /translation="MATKVQDTTTHEAPPPPSSLLIALELRAPWELWSVLPSWPLLAR
                     APAGDGHPVIVFPGLSASDGSTLPIRGYLQNLGYDISGWNQGYNFGPRAGILQTAKRQ
                     VMDTCEVTGEKVSLVGWSLGGIYARELAKELPDCVRSVVTMGTPFSGSHTSTNAWRLF
                     ELTSGKDITRELQQFDLPVAPPVPTTSIYSRTDGVVAWPASIQKPSKSNPRTENVEVI
                     ASHIGLGLNPTAWWALADRLAQPEGQWKPFERRNALQRLIFPDPQR"
     gene            complement(3644..4180)
                     /gene="ppa"
                     /locus_tag="PKX82_06970"
     CDS             complement(3644..4180)
                     /gene="ppa"
                     /locus_tag="PKX82_06970"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOF52031.1"
                     /translation="MSLDNVTPGTKLPEAFNVIIEIPMNADPVKYEVDKASGAIFVDR
                     FMSTAMHYPTNYGYVPKTISGDGDPVDVLVITPVPLIPGVVVTCRAIGILKMEDEAGM
                     DGKVLAVPINKILSLYSRWQKPEDLQPLRLKTIAHFFEHYKDLEEGKWVKVLGWEGPE
                     AAHQEIMDGIANYQKSLA"
     gene            4404..>5036
                     /locus_tag="PKX82_06975"
     CDS             4404..>5036
                     /locus_tag="PKX82_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOF52032.1"
                     /translation="MTGNSALILALVCGFIAVAYGFWARSWILSQDAGNARMQEIAAA
                     IQTGAAAYLARQYKTIAIVGVVLTILIAVFLDGLSAVGFVIGAVLSGACGFIGMNVSV
                     RANVRTAQAATKGIGPALDVAFRGGAITGMLVVGLGLLGVTAFYWFLTGNGNMTPDKN
                     LAQMLNPLIGFAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDD"
BASE COUNT         1027 a         1571 c         1424 g         1014 t
ORIGIN      
        1 ccggggtcat tgccttgatt ccttgcgcac ccagtttgct tctattttta tagcaccctg
       61 ggcacgatag acgagggttg gaaacagttt ttctctaaaa ctcggtgccc gaccagccac
      121 agatccgtcc gcgttcagcg caaatgaatg gccctcaaac actacctcgt cctgcccacc
      181 caccaaatgg gcgtagacca tcggcagccc ggtggcgcgt acgcgctgcg ccatcatctc
      241 ttcgcgctcg tagcctttgc ccacatggaa aggcgatgca ttgatgacgg ccaacatctg
      301 cgcccccgcc tctttggcca gccgcgcggg ctcttcaaac caggcgtcct cacaaatcag
      361 caggccgacc tgcaccaggc tctccgcctc accggcctca aaaacacaca cgccctgccc
      421 gggcgtgaaa taccggcgct cgtcaaatac ctggtagttg ggcaattccc gcttggcgta
      481 ggtggcgacc acctgccctt cgcgcaggac gctggccgca ttgaaccggc tttgtaccgc
      541 caccgagcgg gtacgactgt ccccacccgt ggggtggccc accacgatgg ccatgccttt
      601 taacccggcg gtctgccggg ccactgcttt cacggcatca tcacaggcgg cgatgaacgc
      661 ggggcgcaaa aacaggtctt ccgccgcgta gccacaaatg gacaactccg gtgtcagcag
      721 cagacgcgcg ccctcggcgt aggcctgggt ggctgcatcg acaatttttt tgacgttccc
      781 gtccagatca ccgacgatga agttgagttg ggcaacgcac agtttgagag tcatgaacca
      841 gcaatatttt gttcacagcc gcaccagaag cacggccatt gcgcagaagg cgcaccatgg
      901 gtagcggcaa ttatgtcatc cgggtgcaaa cactgccgtc ccaggtggat gcgtctgcgt
      961 gggatgctct gctacaaaag caatcccagc ccagcccgtt catgcgccac gcctacctgg
     1021 cggcgctgca cagcagccaa agcgcctgtg cagccaccgg ctgggcgccc cacttcatca
     1081 ccctggaacg ccatggcgct ctggaagccg cctgcgtggt gtatctcaaa aaccattctc
     1141 acggcgagta cgtattcgat tcagcatggg ccagcgccta tcgccagcat ggtgtgccct
     1201 actaccccaa agcggtggtg gccgtgccct tcactccggt tccaggcact cgcctgctgg
     1261 cccgtgacaa cgcggcgcga acggctctgg tgcacgcact catcgcttgg tgcaagcaac
     1321 aagccgtatc gtccctgcac ctgttgtttg cggcggaaga agacctggca gcatgtgctg
     1381 gcgcgggttt gatgctgcgc catacggtgc agtttcactg gaccaacact tcgcccggtt
     1441 acgctgactt cgatgccttt ctggccaccc tggcccagga caagcgcaag aagatccggc
     1501 aggaacggcg caaggtggcc gatgcaggcg tcaccttccg gcaggtacag ggcagcggaa
     1561 tcaccaccga ggactgggat tttttctacc gctgttatga gcggacctac ctggagcacg
     1621 gtaacccgcc ctacctcacc cgggacttct ttagccgcat ggagagcacc atgccagaga
     1681 actggctgct cttcgtcgca cagcgcaatg gacagaacat tgctagcagt ttgatagcta
     1741 ttgacgcaca ttccacgggg gctagaggcc aaaatggcac tgaatccgcc aagatagcct
     1801 atggccgcta ctggggtgcg ctggagtccg tcgactgcct gcactttgaa gcctgctact
     1861 accagccgct gcaatggtgt attgcccatg gctaccagcg ctttgagggg ggagcacagg
     1921 gcgaacacaa gatggcccgc gccctgttgc cggtcaggac cagcagtgcg cactggctgg
     1981 cacacccggc attttcagac gcggtatccc actttctgga acgtgaaggc gcgggcgttg
     2041 ccaagtattt acaggacttg gaggatcgaa atccgtttcg tcagactggc ggagcgcagt
     2101 cctgagttac cttgcaatgc ccaactcggc attggacaac aaagcctcga cgttcatgac
     2161 aatcagggtg cgctcgccca atgtgaccaa tcccaggatg aaccgggaat caataccgga
     2221 atcgaactgc ggcgctggct tgatggtggc gctgtccagc gtgaccacat ccgaaaccgc
     2281 gtccaccacc gccccgacca ccgctccgtg gatattgaga atgatgacca ctgtgaattc
     2341 gttgtactgc gcatcaggca catggagctt gagccgcaaa tccgcaatag ggacgatcac
     2401 cccgcgcaaa ttcaccacgc ctttgatgaa gctcggagag ttcaccatgc gggtcggctc
     2461 ctcgtacgaa cggatttcct gcacccgcag gatgtcaatg gcgtattcct cggagcccag
     2521 gcgcagcgtc aagtactgac ccgaatcggc cagaggcttg cctcccgatg cggcaccacc
     2581 ggctgaagac ggcgaattca taatttccat ggcatgtcct gtccttgacg acgatctgcc
     2641 gcaacgccgg cagggggcgt cggggatcat acacggcact cccaaacagt cgcctaggtg
     2701 acgcaaatcc tgagggatcc ctctaatgcc agcacaacga cacgcagcac tgcacaaaag
     2761 ctgctaccgt taaaaccctg ttcaaaacgc ggatttggct cgtcaagacc agcaccaacg
     2821 atacatggca accaaagtgc aagacaccac cacacacgag gcgccccctc cacctagttc
     2881 gctgctgatc gcactggagt tgcgcgcccc ctgggagttg tggtccgtgc tacccagctg
     2941 gccgctactg gcccgcgctc cggctggaga cggccatccg gtcatagtgt ttcccggcct
     3001 gtccgccagt gacggttcca ccttgcccat tcggggctat ctacaaaacc tcgggtacga
     3061 catctcgggc tggaaccagg ggtacaactt cggcccccgt gccggcatcc tgcaaacggc
     3121 caagcggcag gtcatggaca cctgcgaagt caccggggaa aaagtgagtc tggtcgggtg
     3181 gagcctgggt ggcatttatg cgcgtgagct cgccaaggag ttgcctgact gcgtccgcag
     3241 cgtggtgacc atgggtacgc cattttcagg ctcgcacacc agcaccaacg cctggcgcct
     3301 gtttgaactg accagtggca aggacatcac ccgcgaactg cagcagtttg acctacccgt
     3361 cgcaccaccg gtgcctacca cttcgatcta ctcacgcacc gatggcgtgg tggcttggcc
     3421 cgccagcatc cagaagccca gcaagagcaa tcccagaacc gaaaacgttg aagtgattgc
     3481 cagccatatt ggcctggggc tcaatcccac tgcctggtgg gctctggcgg accgtctggc
     3541 gcagccagaa ggtcaatgga aaccgtttga gcggcgtaac gcgctgcagc gcctgatctt
     3601 tccagatccc cagcgctgag cgcatggcgc tcaggctggc aatttaagcc agcgacttct
     3661 ggtagttggc gataccgtcc atgatttcct ggtgggcggc ttcggggcct tcccagccca
     3721 gcaccttgac ccacttgcct tcttccagat ccttgtagtg ctcaaagaag tgggcaatgg
     3781 tcttgaggcg cagcggctgc agatcctcag gcttctgcca acggctgtac agagacagaa
     3841 tcttgttgat cggcacggcc aggaccttgc cgtccatacc ggcttcgtct tccatcttca
     3901 gaataccgat cgcgcggcag gtcaccacca cgccaggaat caggggcacg ggcgtgatca
     3961 ccagcacatc caccgggtcg ccgtcgccag aaatggtctt gggcacataa ccgtagttgg
     4021 tggggtaatg catggccgtg ctcatgaaac ggtccacgaa gatcgcgcca gaggccttgt
     4081 ccacttcgta tttcacagga tccgcattca tcggaatttc aatgatcacg ttgaacgctt
     4141 cggggagctt ggtgccaggg gtaacgttgt caagggacat ggatgttctc tttaatgatg
     4201 aaaatcaact gatgtaagga ttaaccctga ttttactgac tggacacttg ctatttcact
     4261 gtccggcagt tttttccagt tacagtccgg ccgttggcca atcatctcca ccgcagtgtg
     4321 ggttcggagc attgaggaag caacgcagcg gggacagcgc tgacgttgtt ccctccttcg
     4381 cgaaacaaca caggagaatg tttatgacgg gcaactcggc gctgattctg gcgcttgtgt
     4441 gtggcttcat cgccgtggca tacggctttt gggccagaag ctggattctt tcccaggacg
     4501 cgggcaatgc ccgcatgcag gagattgcag cggctatcca gacgggcgct gcagcctatc
     4561 tggcacgcca atacaagacc attgcgattg tcggcgtggt actcaccatt ttgatagcgg
     4621 tattcctgga tggcctcagt gccgtgggct tcgtcatcgg tgccgtactg tccggagcct
     4681 gcggtttcat tggcatgaac gtctcggtgc gcgccaacgt gcgcaccgcg caggcagcca
     4741 ccaaaggcat tggcccggca ctggacgtcg ccttccgcgg tggtgccatt accggcatgc
     4801 tggtggtcgg tctgggactg ctcggcgtca ctgctttcta ctggttcctg acgggcaacg
     4861 gaaacatgac ccccgacaag aacctggccc agatgctcaa ccccctgatc ggttttgcct
     4921 tcggctcttc cctgatttcc atctttgccc gtctgggcgg tggcattttc accaagggcg
     4981 ccgacgtcgg cgcggacctg gtcggcaagg tcgaagccgg cattccggaa gatgac
//
LOCUS       DAOSJM010000163         5027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14890_length_5027_cov_4.119268, whole genome shotgun sequence.
ACCESSION   DAOSJM010000163 DAOSJM010000000
VERSION     DAOSJM010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14890_length_5027_cov_4.119268"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKX82_06980"
     CDS             complement(<1..451)
                     /locus_tag="PKX82_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HOF52033.1"
                     /translation="MLYQLYETQRSLMEPFSDLALAAAKLYGNPLSVAGQNPFAQRIS
                     AGYDLMHRLGKDYEKPEFGLRTIDVDGVDVAIHERVEINKPFCELRRFKRFSDDNKTL
                     QKTKDQPAVLIVAPLSGHYATLLRDTVKTMLKDHKVYITDWKNARLVP"
     gene            complement(504..1850)
                     /locus_tag="PKX82_06985"
     CDS             complement(504..1850)
                     /locus_tag="PKX82_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52034.1"
                     /translation="MKKLGTTLTLLAGLATSQVFAWGNHSFPAYRAFEKMPELAQAQV
                     VTVEPLESFLRAEEKTLEALLASQEAWAAANLEKYPARPAMLAFKADPACSDDARRLA
                     FLMALRVAPNAKLALYYQPDPLNPTAHGPVLPYAAVNTLPEPAKPTQRFIGLKPGDTI
                     SPLVVLASASDEPDYGLDINLWEDSPSDWGKVYGFGKLPFGNPALSYSTQAPFHMGFM
                     HESAVLYAAAPFLKRTFPLLRSYQYTSLAALAFRTGHPYWGWRFTGLSLHYLQDLTQP
                     YHASLAPGESTLKLLAANALAMAGMSGMKNDMVVLLSNRHLALEKYQTELLHAAAIAK
                     KDTAIEQSLRNQDRDRSYPDWNDRYVRDTVSAQSAAAGAPLVATLVATMPPGYVSDPA
                     FDFGVKEDGINLTAELAQRTPADRARLDAAIADLLGNFGAHSRNAVRGILKASPQP"
     gene            1893..2975
                     /locus_tag="PKX82_06990"
     CDS             1893..2975
                     /locus_tag="PKX82_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52035.1"
                     /translation="MLSFQRTVVLLLAVLWAGGSSACTICAPADGQSTLVRQLAAADH
                     IVMAQWTGRGQARVQAVVRASVTVGTPVGADWAPGLPAPATVHPDDSVLLVMPSGGSS
                     WQALGYLPLARLPWLRRVAALQPAVPTAEGSQARAELSVDDLEDRYPLVAQFAYDEIA
                     VQPYAVLRAFASRMDGKRVAQWVKDDARSARFPLYYLLLGLTGADGDAVALTQQLEQG
                     RRATHGPELSAMLAAVVALRRDAGLAWVERYYLAARQTSDADVQAALLALSVHGTDGA
                     AVSKEQVVAAYARYIVRNPHRAGFVASDLANWGRWEFAEAFGAALRSGDTQVFSSRYA
                     MVFYLLRNPRPQAKALVETLRAEKLM"
     gene            complement(3050..4258)
                     /locus_tag="PKX82_06995"
     CDS             complement(3050..4258)
                     /locus_tag="PKX82_06995"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOF52036.1"
                     /translation="MSLFSAVEMAPRDPILGLNEQFNADTNPNKVNLGVGVYFDDNGK
                     LPLLQCVQAAEKTMMDKPTARGYLPIDGIVAYDNAVKALVFGADSEPVTSGRVATVQA
                     IGGTGGLKIGADFLKKVSPNAKVLISDPSWENHRALFTNAGFEVGTYAYFDATANGGK
                     GGVNFEGMLASLNAAAAGTIVVLHACCHNPTGYDITPAQWDQVIAVVKAKNLTPFLDM
                     AYQGFGYGIAEDGAVIQKFVASGLNFFVSTSFSKSFSLYGERVGGLSVLCQDKEEAGR
                     VLSQLKIVIRTNYSNPPTHGGAVVAAVLNNPELRALWEKELGEMRVRIKAMRQKLVDG
                     LKAAGVKQDMSFITTQIGMFSYSGLSKDQMVRLRSEFGVYGTDTGRMCVAALNSKNIE
                     YVCQAIAKVM"
     gene            complement(4298..>5027)
                     /locus_tag="PKX82_07000"
     CDS             complement(4298..>5027)
                     /locus_tag="PKX82_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF561 domain-containing protein"
                     /protein_id="HOF52037.1"
                     /translation="GRNPFSEETAALVEAFRPAVVSFHFGLPHTDLLQRVKAWGAKVL
                     SSATTVEEALWLQEHGADAIIAQGLEAGGHRGMFLSDNITTQVGTFALLPQIVQAVNL
                     PVIAAGGIADARGVAAAMALGASAVQVGTAYMLCPEATTSALHRAALQSPVAQHTALT
                     NLFTGRPARGIVNRVIRELGPLSEATPQFPLATTAMAPLRAKAEAAGRSDFSPLWSGQ
                     NASGCKSIPAAELTHQLAALLPHR"
BASE COUNT          868 a         1423 c         1696 g         1040 t
ORIGIN      
        1 ggggcaccag tcgggcgttt ttccagtcgg tgatgtagac cttgtggtcc ttgagcatgg
       61 tcttgacggt gtcacgcaac agcgtggcat agtgacctga caagggcgcg acgatcagta
      121 cagcaggctg gtctttggtt ttttgcaggg tcttgttgtc atcgctgaaa cgcttgaagc
      181 ggcgcagttc gcaaaatggc ttgttgattt ccacccgctc gtggatggcg acatccacgc
      241 cgtccacatc aatggtgcgc aagccgaatt cgggtttttc gtagtccttg cccagacggt
      301 gcatcaggtc atatccggca gaaatacgct gagcaaacgg gttctgtccg gccaccgaca
      361 gggggttccc gtacagtttg gcagcggcca aagccagatc ggaaaacggc tccatcaggg
      421 aacgttgggt ctcgtagagc tggtacagca tgaaaatctc gctttcggaa atgttgcagt
      481 gcaatataac agtattggct gactcaaggc tgaggacttg ccttgaggat gccgcgcacc
      541 gcattgcggc tgtgtgcgcc aaaattgcct aaaaggtcgg caatggctgc gtccagccgc
      601 gcgcggtcgg ccggcgtgcg ctgggccagc tcggcagtca ggttgatgcc gtcttccttg
      661 acaccaaagt caaatgccgg atctgatacg taacccggag gcatggtggc caccagtgtg
      721 gccaccagcg gggcgccggc agcggcagac tgcgcggata cggtgtcgcg cacataacgg
      781 tcattccagt ccgggtagct gcggtcgcgg tcctgattgc gcagcgattg ttcgatggcg
      841 gtgtctttct ttgcaatagc tgccgcgtgc agcagttcgg tctggtattt ttccagtgcc
      901 aggtggcggt tggaaagcag gacaaccatg tcgtttttca tgccggacat gcccgccatg
      961 gccagggcat tggctgccag gagcttcagt gtggactcgc ccggcgcaag gctggcgtgg
     1021 taaggctgtg tcaggtcctg caggtagtgc agcgacaggc cggtaaagcg ccagccccag
     1081 taaggatgtc cggtgcgaaa ggccagcgct gccaggctgg tgtactggta ggagcgcagc
     1141 agggggaatg tccgcttgag gaatggtgcc gccgcataca gcactgcgct ctcgtgcatg
     1201 aagcccatat ggaagggggc ctgtgtggaa taggacaggg cagggttgcc aaacgggagc
     1261 ttgccgaacc cgtagacctt gccccagtcg gaggggctgt cttcccagag gttgatatcc
     1321 aggccatagt ctggctcgtc cgaggccgat gccagcacca ccagcgggct gatggtgtca
     1381 ccgggtttga ggccaatgaa gcgctgtgtg ggtttggcag gctcgggcag ggtgttgacc
     1441 gctgcatagg gcagtacagg gccatgggct gtcgggttga gcgggtccgg ctggtagtag
     1501 agcgccagtt tggcattggg cgccacacgc aaggccatca ggaatgccag ccgtcgggca
     1561 tcatcggaac aggcgggatc ggccttgaac gcgagcatgg ccggtcgtgc agggtacttt
     1621 tccagattgg ccgcggccca ggcttcctgg ctggccagca gggcttccag cgttttttct
     1681 tcggctctga ggaagctttc cagcggttcc acggtgacaa cctgggcctg agccagctcc
     1741 ggcatttttt caaatgcccg gtaggcagga aagctgtggt tgccccatgc gaaaacctgt
     1801 gaagtcgcca gtccggcaag caacgtgagc gtggtgccca gttttttcat tgtgtcgtct
     1861 cctgtctgtt ctacgctgga ttatgctgcg gcatgctttc attccaacgc accgtggttt
     1921 tgctgctcgc cgtgctctgg gccggcgggt ccagtgcctg cacgatttgt gcacccgccg
     1981 atgggcaaag tacgctggta cgccaactgg ctgcagcgga ccatattgtg atggcgcaat
     2041 ggacgggccg aggccaggcc agggtgcagg ccgttgtccg cgcaagcgtg acggttggta
     2101 caccggttgg cgcagactgg gcgcctggtc tgcctgctcc ggctacggta catcccgatg
     2161 attcggtgtt gctggtgatg ccatctggcg gttcttcatg gcaggccttg ggttacctgc
     2221 cgctggccag attgccctgg ctacgccgtg ttgctgcatt gcagcccgct gtacccactg
     2281 cagaaggctc tcaggcgcgg gctgaactgt cggtggatga tctggaggac cgttaccccc
     2341 tggtagccca gtttgcctac gatgaaattg cggtgcagcc ctacgccgta ttgcgggcat
     2401 ttgcctccag gatggatgga aagcgcgtgg cgcagtgggt aaaggacgat gcgcggtccg
     2461 cgcggtttcc gctgtactac ctgctgctgg ggctgacggg ggcggacggt gatgcggtgg
     2521 ccctgacgca acaactggag caggggcgcc gtgccaccca tggaccggag ctgtccgcca
     2581 tgctggccgc cgttgtggcc ttgcgccgcg acgccggatt ggcgtgggtg gagcggtatt
     2641 acctggcggc ccgccagacc agtgatgccg atgtgcaagc cgccttgctg gcgctcagtg
     2701 tgcatggcac ggacggtgcc gcggtttcta aagagcaggt ggttgccgcc tacgcccgct
     2761 acattgtgcg caacccgcac cgggcaggtt ttgtggcgtc ggacctggcc aactgggggc
     2821 gctgggaatt tgccgaggcc tttggcgcgg ccctgcgttc cggcgataca caggtgttct
     2881 cttcgcgcta cgcgatggtg ttttacctgt tgcgcaatcc gcgcccgcaa gccaaggcct
     2941 tggtggaaac actgcgggcc gagaaactga tgtagtttcc cggcccgaaa atgctatcta
     3001 tttgatagct gcttacgcaa tatggacggg ggctaacccc cgtttttgct tacattacct
     3061 tggcaatggc ctggcagacg tactcaatgt tcttgctgtt gagcgcggcc acgcacatgc
     3121 ggccggtgtc ggtgccatag acgccgaatt cggaccgcag acgcaccatc tggtccttgg
     3181 agagaccgga gtagctgaac atgccgatct gggtggtgat gaaggacatg tcctgcttta
     3241 cgccggcagc cttcaatcca tccaccagct tctggcgcat ggccttgatg cgcacgcgca
     3301 tttcgcccag ctctttttcc cacagcgcgc gcagctcggg gttgttcagc accgcagcca
     3361 ccacggcacc accatgggtg ggagggttgg agtagttggt gcggatcacg atcttgagct
     3421 ggctcagcac gcgaccggct tcttccttgt cctggcacag caccgacagg ccgcccacgc
     3481 gctcgccgta caggctgaag ctcttggaga aggaggtgga gacaaagaag ttcaggccgg
     3541 aggccacaaa cttctggatc acggcgccat cttcagcaat gccgtagcca aagccctggt
     3601 aggccatgtc caggaagggg gtcaggttct tggccttgac caccgcaatc acctggtccc
     3661 attgtgcggg cgtgatgtca tagccggtgg ggttgtggca gcaggcgtgc agcacgacga
     3721 tggtgccggc agcggcagcg ttcaggctgg ccagcatgcc ttcaaagttc acaccgccct
     3781 tgccaccgtt ggctgtcgcg tcgaagtacg cataggtgcc cacttcgaaa ccggcattgg
     3841 tgaacagcgc gcggtggttc tcccagctgg ggtcggaaat cagtactttg gcgttggggc
     3901 tgactttctt caggaagtcg gcaccgatct tcagaccacc ggtgccgccg atggcctgca
     3961 ccgtggctac gcggccggag gtgacgggct cgctgtccgc accaaagacc agggctttga
     4021 cagcgttgtc gtaggccacg atgccgtcga tgggcaggta gccgcgcgcg gtgggtttgt
     4081 ccatcatggt cttttcagca gcttgcacgc attgcagcag gggcagcttg ccgttgtcgt
     4141 cgaaatacac gccaacgccc aggttcacct tgttggggtt ggtgtcagcg ttgaattgtt
     4201 cgttcagacc caggatgggg tcgcggggag ccatttcgac ggcggaaaag agagacatgg
     4261 acggatcctg tgtgggggtt gggggaaacc cggtatttta ccggtgcggc agcaaggccg
     4321 ccagttggtg tgtcagctcg gcagcgggaa tacttttgca gccactggcg ttctggccgc
     4381 tccagagcgg cgagaaatcg ctacggcctg cggcttcagc cttggcgcgc aggggcgcca
     4441 tggcggtggt ggccagcgga aactgcggcg tggcttcgct cagcgggccg agttcgcgaa
     4501 tgacccggtt gacgatgccg cgcgcggggc ggccggtaaa caggttggtc agcgccgtgt
     4561 gctgggccac ggggctttgc agtgcagccc tgtgcagggc gctggtggtg gcttccgggc
     4621 acagcatgta ggcggtgccc acctggacgg cgctggcgcc cagcgccatg gcggctgcca
     4681 cgccccgggc atcggcaatg ccgccggccg cgatgacggg caggttcacc gcctgcacga
     4741 tctggggtaa caaggcaaag gtcccgacct gtgtggtgat gttgtcactg agaaacatgc
     4801 cccggtggcc accggcttcc agtccctgcg cgatgatggc atcggcaccg tgctcctgta
     4861 gccagagggc ttcttccacg gtggtggcgg aggacagcac cttggcgccc caggctttga
     4921 cccgctgcaa caggtccgtg tggggcagcc caaagtggaa gctgaccaca gcgggacgaa
     4981 acgcttcgac cagtgcggca gtctcttcgc taaacgggtt gcggccc
//
LOCUS       DAOSJM010000164         5008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_14961_length_5008_cov_3.965072, whole genome shotgun sequence.
ACCESSION   DAOSJM010000164 DAOSJM010000000
VERSION     DAOSJM010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14961_length_5008_cov_3.965072"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKX82_07005"
     CDS             complement(<1..304)
                     /locus_tag="PKX82_07005"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HOF52038.1"
                     /translation="MAHPDDSVNDRISALVDGQLRGAEFSEAMDSLASQPEDERAWQM
                     YHLIGDVLRSEELAGGAHDRDFLSRLEKKLAQEPVRTASASTRVFHAQGTSANAGAW"
     gene            complement(316..939)
                     /gene="rpoE"
                     /locus_tag="PKX82_07010"
     CDS             complement(316..939)
                     /gene="rpoE"
                     /locus_tag="PKX82_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HOF52039.1"
                     /translation="MNPKDAASAEDSDLLLVERANAGDQRAFGLLVLKYQRRVQRLIA
                     RMVRDVDLVDDIAQETFIRAYRALHQFRGDAQFYTWLYRIAVNTAKKALLQLKRDPTV
                     SESFLSKDEDDETSWQKNEPTSEETPETVFAAKEIAAVVNAAMEALPEDLRLAVTLRE
                     IEGLSYDEIALAMDCPIGTVRSRIFRAREAISAKVKPLLDKQTGKRW"
     gene            complement(936..1346)
                     /locus_tag="PKX82_07015"
     CDS             complement(936..1346)
                     /locus_tag="PKX82_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52040.1"
                     /translation="MLATLWSVALCVSVLLLQTPLAPEALWSILLALGLSCALSFAGW
                     IRSDTGTLQWDGQHWLWPGFGEAPVQRMRLVLDFQKCILLQLRSEDGGVAWLWAQAGT
                     SHHQWLAVRRAVVAAQRTRREPELRQHLLDGEKG"
     gene            complement(1432..2676)
                     /gene="fabF"
                     /locus_tag="PKX82_07020"
     CDS             complement(1432..2676)
                     /gene="fabF"
                     /locus_tag="PKX82_07020"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOF52041.1"
                     /translation="MSRRRVVVTGLGCVSPVGNTVTEAWSNVLAGKSGIGAITKFDAS
                     AFSCKIAGEVRDFDLESYIGSKEARTMDTFIHYGIAAAAQAVADAGLATGDALGEEEA
                     TRIGCVIGSGIGGLPLIESTHAEYVARGPRRISPFFVPASIINMTAGHVSMRFGFKGP
                     NIAIVTACTTGLHCIGEAGRMIEYGDADVMVAGGSEATVSPLGMGGFASMRALSTRND
                     DPATASRPWDKDRDGFVLGEGGGVMVLEEYEHAKARGARIYAELAGFGMSADAGHMTA
                     PNMDGPRRAMVGALRNAGVNADQVQYLNAHGTSTPLGDLNETNAIKAALGDHAHKIVV
                     NSTKSMTGHLLGGAGGLESVFTVLAIHHQKSPPTINIFNQDPECDLDYCANTARDMRI
                     DVAVKNNFGFGGTNGTLVFKRI"
     gene            complement(2694..2933)
                     /gene="acpP"
                     /locus_tag="PKX82_07025"
     CDS             complement(2694..2933)
                     /gene="acpP"
                     /locus_tag="PKX82_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOF52042.1"
                     /translation="MSDIEVRVKKIIAEQLGVEESQVTNEKAFVADLGADSLDTVELV
                     MALEDEFGIEIPDEDAEKITTVQNAIDYATSHKKA"
     gene            complement(3055..3801)
                     /gene="fabG"
                     /locus_tag="PKX82_07030"
     CDS             complement(3055..3801)
                     /gene="fabG"
                     /locus_tag="PKX82_07030"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOF52043.1"
                     /translation="MSEVKFEGQVALVTGASRGIGAAIALELARKGVKVIGTATTDAG
                     AIKIREALEAFPGCDGRVLNVTDAAASEALVDAIAKEHGAVHILVNNAGITRDTLAMR
                     MKDDDWDAVMDTNLKGVFRMSRAVIRPMMKQRYGRIVNITSVVGASGNPGQANYAAAK
                     AGVAGMTRALARELGSRNITVNCIAPGFIETDMTASLGEDQQKALLGQIPLGHLGKPT
                     DIAHAVAYLASPMAGYVTGQEIHVNGGMYM"
     gene            complement(3798..4748)
                     /gene="fabD"
                     /locus_tag="PKX82_07035"
     CDS             complement(3798..4748)
                     /gene="fabD"
                     /locus_tag="PKX82_07035"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOF52044.1"
                     /translation="MKQFAFVFPGQGSQAVGMLDAWGDHPVVAQTIREASDALGEDIG
                     KLIHEGPKEALALTTNTQPVMLVAGVAAYHVWMSEVGVAPAVVAGHSLGEYSALVASG
                     VLSLAQAAPLVRLRAQAMQEAVPVGVGAMAAILGMDAAKVISGCAEATATFGANSLEV
                     VEAVNFNDPLQTVIAGSKAAVDKACEILKANGAKRALPLPVSAPFHSSLMKPAAEKLK
                     ERLEDLELGSPQIPLINNIDVAIEVDADRIRDALYRQAFGPVRWVECVQAIKARGLTH
                     VVECGPGKVLAGMVKRIDAELVGAALFDPATLADVQGVLA"
     gene            complement(4803..>5008)
                     /locus_tag="PKX82_07040"
     CDS             complement(4803..>5008)
                     /locus_tag="PKX82_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HOF52045.1"
                     /translation="MQSTARKLKMSMDKVVVTVDQHGNTSAASIPLALDFAVRSGKVK
                     PGETLMLEGVGGGFTWGAVLLNL"
BASE COUNT         1035 a         1599 c         1374 g         1000 t
ORIGIN      
        1 gccaggcacc ggcattggca ctcgtacctt gcgcatgaaa cacccgggtc gatgcagatg
       61 ccgtacgcac aggctcctgt gccagcttct tttccagcct ggacagaaaa tcgcgatcgt
      121 gtgcgccccc ggcaagctct tcactgcgca aaacgtcgcc aatcaggtgg tacatctgcc
      181 aggcgcgttc atcctccggc tgggaagcca gactgtccat cgcttcggaa aattctgcgc
      241 cccgcaactg cccgtccacc aaggcagaga tgcggtcgtt cacagagtca tcagggtgtg
      301 ccatctcaat catcctcacc agcgcttgcc ggtctgtttg tccagaagcg gtttcacctt
      361 ggccgaaatc gcctcgcgcg cccggaagat ccgcgaccgg accgttccga tggggcaatc
      421 catggccaga gcaatctcgt cataactcaa tccctcaatc tcgcgcaggg tcactgccaa
      481 acgcaaatcc tccggcaagg cttccatggc cgcgttgaca accgctgcaa tttccttggc
      541 cgcaaaaact gtctcagggg tctcttcgct ggttggttcg tttttctgcc aggaagtttc
      601 atcgtcctcg tctttagaca ggaaactctc cgaaacggtg gggtcacgct tgagctgcag
      661 cagagctttc tttgcggtgt taacggcaat ccggtacagc caggtataga actgcgcatc
      721 cccacggaac tggtgcaagg cccggtaggc ccgaatgaag gtttcctgtg cgatgtcatc
      781 caccagatcc acatcccgca ccatgcgggc aatcaggcgt tgtacgcggc gctggtattt
      841 cagcaccaac agtccaaacg cccgctgatc gccagcattg gcacgctcca ccagcaacag
      901 gtcagaatct tctgccgacg ctgcgtcttt cgggttcacc ccttttctcc atccagcaga
      961 tgctgacgca actctggctc acgcctggtc cgctgtgccg caaccacggc tctgcgcacg
     1021 gccagccatt ggtggtgcga ggtgccagcc tgcgcccaaa gccaggcaac accgccatct
     1081 tcgctgcgaa gttgcagaag aatacatttc tggaaatcca gtaccaaccg catccgctgc
     1141 accggtgcct caccaaaccc cggccacagc caatgctggc cgtcccactg caaagtgccg
     1201 gtatcggacc gaatccagcc cgcgaaagac agcgcacagg acaaccccag cgccaacagg
     1261 atgctccata gcgcctccgg cgcaagcggg gtttgcaaca gcagtactga cacacacagc
     1321 gctacgctcc agagcgtggc cagcatgccg aaataccatt gggaccgccc tatgtggtga
     1381 ctgaccgcag gagggccgtg catagggggt gaaaagcgga gaggctctac ctcagatgcg
     1441 cttgaaaacc agggtaccgt tggtgccacc aaaaccaaag ttgttcttga cggctacatc
     1501 aatgcgcata tcgcgagcgg tgttggcgca gtagtccaga tcgcactcgg gatcctgatt
     1561 gaagatattg atcgtgggag ggctcttctg gtggtggatc gccagcaccg tgaacaccga
     1621 ctccaagcca cccgcaccgc ccagcaagtg gccggtcatg gacttggtgg aattgaccac
     1681 gatcttgtgt gcatggtcac ccagcgccgc tttgatggcg ttggtttcgt tcagatcacc
     1741 cagtggcgta gaggtgccat gggcattgag gtactgcacc tggtcggcat tgacaccggc
     1801 attgcgcagc gcacccacca tggcgcggcg tgggccatcc atattgggtg cggtcatgtg
     1861 accggcatcg gcactcatac caaaaccggc cagctctgcg taaattctgg cgccgcgggc
     1921 cttggcgtgc tcgtactctt cgagcaccat cacaccgcca ccttcaccca gcacgaagcc
     1981 gtcacggtcc ttgtcccagg gacgggacgc ggttgcagga tcgtcattgc gggtgctcag
     2041 ggcgcgcatg gaagcaaagc cgcccatgcc cagcggtgac acggtcgctt cggagccacc
     2101 agccaccatc acatcagcgt cgccgtactc gatcatccgt cccgcttcgc caatgcaatg
     2161 cagacctgtg gtacaggctg tcacgatggc aatgttgggg cccttgaagc caaaacgcat
     2221 ggacacatgt ccggccgtca tattgatgat ggaggcgggt acaaaaaacg gagaaatccg
     2281 gcggggcccg cgggcgacgt actccgcatg ggtgctttca atcagcggca agccgccgat
     2341 tccagaaccg atgacgcagc cgatccgcgt agcctcttct tcgcccagtg catcaccggt
     2401 agccagccca gcatctgcca ctgcttgcgc agcggcggca atgccgtaat ggataaaggt
     2461 atccatggtg cgtgcttcct tggagccgat atacgactcc agatcaaaat cacgcacctc
     2521 tccggcaatc ttgcaggaga aggctgatgc atcaaacttg gtaatcgcgc cgatgcccga
     2581 ctttccagcc agaacattcg accaggcttc agtaaccgta tttccaacgg gactgacgca
     2641 acccaaacca gtgactacta cgcggcgacg ggacataggc taacggcctc ttatcaggct
     2701 tttttatgcg aggttgcgta atcgatggcg ttttgcacgg tggtaatttt ttccgcgtct
     2761 tcgtcaggaa tttcaatccc gaactcgtct tcgagtgcca tcaccagttc cactgtgtcc
     2821 agggagtctg cacccagatc ggccacaaag gctttttcgt tggtgacttg agactcttca
     2881 acaccgagtt gctcggcaat gatttttttg acacggactt cgatatcgct catgggttcc
     2941 ctctaagggt tgtaaaagaa tccgagattt tacccgcgct agaggcgaac ctctaaccaa
     3001 gtagccgaac ggaccaatcg gctgattttt ccaacggtgc caagcttaca tggattacat
     3061 gtacatgccg ccattgacat gaatttcctg ccccgtcaca tagccagcca tcggcgacgc
     3121 cagataggca acggcatgcg cgatgtcggt cggcttgccc aggtgtccca gcggaatctg
     3181 gcccagcagg gctttttgtt ggtcttctcc gaggctggcc gtcatatcgg tctcaatgaa
     3241 gccgggagcg atgcaattga cggtaatgtt gcgcgaacca agctcgcgcg ccagtgcacg
     3301 cgtcatgcct gcgacaccgg ccttggctgc cgcgtagttg gcctgaccgg gattacccga
     3361 ggcacccacg accgacgtga tgttgacgat gcgaccgtag cgctgcttca tcatggggcg
     3421 aatcactgca cggctcatgc gaaagacgcc cttgaggttg gtgtccatga ccgcatccca
     3481 gtcatcatct ttcatgcgca tcgccagcgt gtcgcgtgtg atccccgcgt tgttcaccag
     3541 aatgtggacc gctccatgct ccttggcaat cgcatccacc agggcttctg acgcagccgc
     3601 atcggtcaca ttcaggacgc gtccatcgca tccaggaaag gcctccaggg cttcgcggat
     3661 cttgattgca ccggcgtcgg tggtggcggt gccaatgacc ttcacgccct tgcgtgccag
     3721 ctccagggca atggccgcgc caataccgcg cgatgcgccg gtcaccaggg cgacctggcc
     3781 ttcaaacttt acttcgctca tgccaaaact ccttgcacgt cggccagggt ggccggatca
     3841 aacagggctg cgcccaccaa ctcggcgtcg atgcgcttga ccattcccgc cagcaccttg
     3901 ccagggccgc attcgaccac atgggtcagc ccccgcgcct tgatcgcctg cacgcactcc
     3961 acccagcgca ccgggccaaa cgcctggcgg tacagcgcat cgcgaatgcg gtctgcatcc
     4021 acctcgatgg caacgtcaat attgttgatc aagggaatct ggggtgaacc cagttccaga
     4081 tcttccagcc gctccttgag cttctccgcc gcgggcttca tcagactgga gtggaatggg
     4141 gcggatacgg gcagcggcag tgcgcgcttg gcaccattgg ctttgagtat ttcacaggcc
     4201 ttgtccacgg ccgccttgct gccggcaatc acggtttgca gcgggtcatt gaaattgacc
     4261 gcctccacca cttccagcga attggcaccg aacgtcgctg ttgcttcggc gcagccggaa
     4321 atcaccttgg cagcgtccat tcccagaatg gccgccatgg caccgacacc tacgggaacc
     4381 gcttcctgca tggcctgagc ccgcaggcgg accagtggtg cagcctgcgc aagcgagagc
     4441 accccggaag ccaccagggc actgtattca cccagcgagt gccccgccac aaccgcaggc
     4501 gccacaccca cctcggacat ccagacatgg taggcagcca caccagccac cagcattacg
     4561 ggctgggtgt tggttgtcag cgcaagcgcc tccttggggc cttcatggat cagcttgcca
     4621 atgtcctccc ccagtgcgtc ggaggcttcg cgaatggtct gtgccacgac agggtggtcc
     4681 ccccaggcat cgagcatgcc cacagcttga gagccctgac ctggaaatac aaaagcgaac
     4741 tgtttcatac aaggattctt gatcaaattg actggtagcc cccgcgaata ctgcaggagg
     4801 cgctacaaat tcaggagcac agcaccccag gtgaagccgc cacccacgcc ttccagcatc
     4861 agggtctcac cgggcttgac cttgccgcta cgcaccgcga aatccagcgc cagcggaatg
     4921 gaggcagccg aggtgttgcc atgctggtct accgtcacca ccactttgtc catggacatc
     4981 ttgagcttgc gggccgtact ttgcatga
//
LOCUS       DAOSJM010000165         4995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15012_length_4995_cov_4.297368, whole genome shotgun sequence.
ACCESSION   DAOSJM010000165 DAOSJM010000000
VERSION     DAOSJM010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15012_length_4995_cov_4.297368"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PKX82_07045"
     CDS             complement(<1..1439)
                     /locus_tag="PKX82_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HOF52046.1"
                     /translation="MNAPTSLNHLLAAAEPAPHVHERIREIPYNYTSFSDREIVIRLL
                     GAPSWELLNQLRGERRTGRSARMLYEVMGDIWVVQRNPYLQDDLLDNPKRRGQLVDAL
                     NHRLGEVEKRRTPQADPERDAMVGQLLVAARAAVQSFDAVFVEVGALRKKTQKLLYKY
                     TARDNVKFDGLSRVSHVTDATDWRVEYPFVVLTPDSEEEMAGLVKGCIELGLTIIPRG
                     GGTGYTGGAIPLTWKSAVINTEKLEAMSEVEMLRLPGLDREVPTVWSEAGVVTQRVAD
                     AAERGGFVFAVDPTSIEASCIGGNIAMNAGGKKAVLWGTALDNLASWRMVTPQAEWLE
                     VTRLQHNMGKIHDAEVASFELQYFKADGKTKIRSERLDIPGKTFRKEGLGKDVTDKFL
                     AGLPGIQKEGCDGLITSARWIVHRMPSHTRTVCLEFFGNAKDAVPSIVEIKDFMFAEQ
                     KRTGVLLAGLEHLDDRYLKAVGYATKSKRG"
     gene            1608..2927
                     /gene="ugpB"
                     /locus_tag="PKX82_07050"
     CDS             1608..2927
                     /gene="ugpB"
                     /locus_tag="PKX82_07050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HOF52047.1"
                     /translation="MQLNRRALAIAALSAAAFTVPAQAQTDIQWWHSMTAVNGEWVND
                     LAKEFNASQKEYRILPTYKGSYDESMTAAVAAFRAGNAPHILQVFEVGTATMMASKNA
                     IVPVGKVMTDAGQSFDPKAYVPAVAGYYTAPNGQMLSFPFNSSTTVFYYNKDAFKAAG
                     LDPEKAPATWPEVALAAAKLKSNGHKCPISISWQGWTQLESFSAWHNVEFASKQNGLA
                     GMDARLKINSPLHVRHIENLANMAKQGLFVYKGRGNVPEATFVSGECAMMTTSSGFYG
                     NVKKNAKFNFGLAPLPHYQDVPGAPQNTVIGGASLWVMAGKKPDEYKGIAKFFGFLSS
                     PEVQSASHKRTGYLPITTAAFQLTEKSGFYKQNPGTDVAVNQMIRKVTDKSRGIRLGN
                     YVQIRAIEDEELEQVWAGKKTAKEALDAIVKRGDELLERFEKANKAK"
     gene            2933..3820
                     /gene="ugpA"
                     /locus_tag="PKX82_07055"
     CDS             2933..3820
                     /gene="ugpA"
                     /locus_tag="PKX82_07055"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HOF52048.1"
                     /translation="MAAEKRVVFKSSWLPWVLIAPQVVVIAVFFFWPAAQALLQSTQQ
                     SDAFGTSVEWVGLENFRNLWNDESYLASFKTTAIFSVLVATLGLTLSLLLAVFADRVV
                     RGSGVYRTFLIWPYAVAPAVAGVLWLFMFAPSIGIVSYGIRQLGLSWDPLLNSTNAMS
                     LIVMAAVWKQISYNFLFFLAGLQSIPKSLIEAAAMDGARPWRRFWTIQFPLLSPTTFF
                     LLVINIVYAFFDTFAIVDAATKGGPGKDTAILVYKVYFDGFKAMDLGGSAAQSVVLMV
                     IVVALTVVQFRFVEKKVNY"
     gene            3820..4671
                     /gene="ugpE"
                     /locus_tag="PKX82_07060"
     CDS             3820..4671
                     /gene="ugpE"
                     /locus_tag="PKX82_07060"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HOF52049.1"
                     /translation="MVERRPWLDFLSHAILVIGVLVVAFPVYITFVASTHTTQEVVQS
                     PMPLLPGSHFIENYTTALSGDRQGSGSHASVGRMMVVSLVTALVISIGKIAISLLSAF
                     AIVYFRFPFRMAFFWAIFITLMLPVEVRIGPTYQVVADLGLLNSYAGLTIPLIASATA
                     TFLFRQFFLTVPDELVEAARVDGAGPMRFFKDILVPLSRTSMAALFVIQFIYGWNQYL
                     WPLLVTTEENMYPVVIGIKQMISGGDAQTEWNIVMATAMLAMLPPALVVMLMQKWFVK
                     GLVDTEK"
     gene            4671..>4995
                     /locus_tag="PKX82_07065"
     CDS             4671..>4995
                     /locus_tag="PKX82_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52050.1"
                     /translation="MASIELKNVIKRYGAGKGSNQVIHGVNAQIADGEFVVIVGPSGC
                     GKSTLLRMVAGLEEISSGEICIGGRVVNRLEPSERDIAMVFQNYALYPHMTVFENMAY
                     GLKIAK"
BASE COUNT          971 a         1524 c         1464 g         1036 t
ORIGIN      
        1 ccgcgtttgc tcttggtggc gtagcccacc gccttgaggt agcggtcgtc cagatgctcc
       61 agcccggcca gcaacacacc cgtgcgcttt tgctccgcga acatgaagtc cttgatttcc
      121 acaatgctgg gcacggcatc cttggcgttg ccaaagaact ccaggcacac ggtgcgtgtg
      181 tggctgggca tgcggtgcac gatccagcgc gcgctggtga tcagaccgtc gcaaccctct
      241 ttctggatgc caggcaggcc cgccaggaac ttgtccgtca catccttgcc caagccctcc
      301 ttgcggaaag tcttgccggg aatgtccagc cgctcgctgc ggatcttggt cttgccgtcc
      361 gccttgaagt actgcaactc gaagctggcc acttccgcat catggatctt gcccatgttg
      421 tgctgcaggc gggtcacttc cagccactcg gcctggggtg tcaccatgcg ccagctggcc
      481 aggttgtcca gtgcagtacc ccagagcaca gccttcttgc cgccagcgtt catagcgatg
      541 ttaccgccaa tgcacgaggc ctcgatcgag gtcgggtcca ccgcaaacac gaaaccaccg
      601 cgctcggccg catcggccac gcgctgcgtg accacgccgg cttcgctcca gacggtgggc
      661 acttcgcggt ccagtcccgg caggcgaagc atctccacct cgctcatggc ttcgagcttt
      721 tcggtattga tgaccgcact cttccaggtc agcggaatgg cgccgccggt gtagccggtg
      781 ccacctccgc gcgggatgat ggtcagcccc agctcaatac agcctttgac caggcccgcc
      841 atttcctctt cggaatcggg cgtcaggacc acaaagggat actcgacgcg ccagtcggtc
      901 gcatcggtca catggctcac gcgcgagagg ccgtcaaact tgacgttgtc cctggcggtg
      961 tacttgtaga gcagcttctg ggtctttttg cgcagggcac ccacttcgac aaagactgcg
     1021 tcaaacgatt gcacggcagc acgtgcagcc accagcagct ggcccaccat ggcatcacgc
     1081 tcagggtcgg cttgcggggt gcggcgtttt tccacttcac ccaggcggtg gttcagtgcg
     1141 tccacgagct ggccgcggcg cttggggttg tccagcaggt cgtcctgcaa atacgggttg
     1201 cgctgcacca cccagatgtc gcccatcact tcgtacagca tccgtgccga acggccggtg
     1261 cggcgttctc cacgcagctg gttcagcaac tcccaggacg gggcacccaa caggcgaatc
     1321 acgatttccc ggtcggaaaa cgaggtgtag ttgtagggga tttcgcggat gcgttcgtgc
     1381 acatgcggcg ctggttcggc ggccgccagc aggtgattga gcgaggtggg agcgttcatc
     1441 agggtctagt caggcagacc ggagatggta ccgcagcgca gcaatcacac accgcgcata
     1501 gtggtaagcc cttaccaaca gatgaaaagc catcggattt aatgcaactg aagccaattt
     1561 gacatgtgcc atcgctacca tcgcgagatt caatcagagg agtgaccatg caactgaacc
     1621 gcagagccct tgccattgca gccctgtctg cagcggcttt taccgtgccc gcccaagccc
     1681 aaaccgatat ccagtggtgg cactctatga cagccgtcaa cggggaatgg gtcaacgatc
     1741 tggccaagga attcaatgcc agccagaagg aataccggat tcttcccact tacaagggca
     1801 gctacgacga gtcgatgact gcagcggtgg cggccttccg cgccggcaac gcgccccaca
     1861 tcctgcaggt ctttgaagtg ggcacggcca ccatgatggc cagcaagaat gccatcgtcc
     1921 cggtcggcaa ggtcatgacc gacgcaggcc agagtttcga ccccaaagcc tacgtacccg
     1981 cggtggccgg ttactacacc gcccccaacg gccagatgct gagctttccg ttcaacagct
     2041 cgaccactgt cttctattac aacaaggacg ccttcaaggc ggctggcctc gaccccgaaa
     2101 aagccccggc aacctggccg gaagtggcac tggcagcggc caagctcaaa tccaacggcc
     2161 acaaatgtcc catttccatc tcctggcagg gctggaccca gctggagagc ttctctgcct
     2221 ggcacaacgt cgaatttgcc agcaagcaga acggcctggc tggcatggat gcccgcctga
     2281 agatcaactc tcccctgcat gtgcgccata ttgaaaacct ggccaacatg gccaaacagg
     2341 ggctgtttgt ctacaagggc cgcggcaatg tgccagaagc caccttcgtc tcgggtgagt
     2401 gcgccatgat gacgacgtcc agcggcttct acggcaatgt caaaaagaac gcgaagttca
     2461 actttggtct ggcgcccctg ccccactacc aggatgtgcc gggggcaccc cagaacaccg
     2521 tgatcggtgg tgccagcctg tgggtgatgg cgggcaagaa accggatgaa tacaaaggca
     2581 tcgccaagtt cttcggcttc ctctccagcc ccgaagtgca atcggccagc cacaaacgca
     2641 ctggctacct gcctattacc accgcagctt tccagttgac cgaaaaatcc ggcttctata
     2701 aacagaaccc cggcaccgat gtggcggtca accagatgat ccgcaaggtc accgacaagt
     2761 cgcgtggcat ccgccttggc aactatgtgc agatccgcgc gatcgaggat gaagaactcg
     2821 aacaggtctg ggccggcaag aaaaccgcca aagaggctct ggacgccatc gtgaaacgcg
     2881 gcgacgaatt gctcgaacgg tttgagaagg ccaacaaggc aaaatagcct gcatggctgc
     2941 agaaaagcgc gtcgttttca aatcatcttg gctgccctgg gtgctgatcg caccgcaggt
     3001 ggttgtgatc gccgtgtttt tcttctggcc tgcggcgcag gcgctgctgc aatccacgca
     3061 gcagtcggat gccttcggta cctcggtgga atgggtgggg ctggagaatt tccgcaattt
     3121 gtggaatgac gagtcctacc tggcttcgtt caagaccacc gccatcttct cggtgctggt
     3181 cgccacacta gggcttacgc tttcgctgct gctggccgtt tttgcggacc gcgtagtgcg
     3241 gggctccggg gtctaccgca ccttcctgat ctggccctat gcggtggcac cagcggtggc
     3301 tggcgtgctg tggctgttca tgtttgctcc cagcatcggc atcgtgtcct acggcatccg
     3361 gcagctgggg ttgtcgtggg atcccctgct caacagcacc aacgccatgt ccctcatcgt
     3421 gatggcagcc gtgtggaagc agatttccta caacttcctg ttctttctgg ccggactgca
     3481 gtccatcccc aagtcgttga tcgaggccgc agccatggac ggcgcacgcc cctggcgccg
     3541 gttctggacg atccagtttc cgctgctgtc gcctacgacc ttcttcctgc tggtgatcaa
     3601 catcgtctat gcgttttttg acacctttgc gattgtggat gccgccacca agggcggccc
     3661 gggcaaagac acggccattc tggtctacaa ggtgtacttt gatggcttca aggccatgga
     3721 cctgggtggc tcggccgcgc agtcggtggt gctgatggtg atcgtcgtcg cgctcaccgt
     3781 ggtgcaattc cgctttgttg agaaaaaggt gaactactga tggttgagcg gcgcccctgg
     3841 ctggattttc tgtcgcacgc gatcctggtc attggcgtgc tggtggtggc gtttccggtg
     3901 tacatcacct ttgtggcgtc cacccacacc acgcaggagg tggtgcagtc gccgatgccg
     3961 ctattgcctg gcagccattt catagagaac tacaccacgg cgctgagtgg ggaccggcag
     4021 ggcagcggct cacatgcctc ggtggggcgc atgatggtgg tgagcttggt aacggcgctg
     4081 gtgatttcca tcggcaagat tgccatttcg ctgctctcgg cgtttgccat cgtgtatttc
     4141 cggttcccgt ttcgcatggc gttcttctgg gccatcttca tcaccctgat gctgccggtg
     4201 gaggtacgca ttgggccgac ctaccaggtg gtggccgact tgggactact caacagctac
     4261 gccgggctga ccattccgct gattgcctcc gctacggcca cttttctgtt ccgccaattc
     4321 tttctgaccg ttccggatga actggtagag gcagcccgcg tggacggtgc cggaccgatg
     4381 cgctttttca aggacattct ggtgccgctc tcacgcacca gcatggccgc cctgtttgtg
     4441 atccagttca tctatggctg gaaccagtat ctttggccgc tgctggtgac gaccgaagaa
     4501 aacatgtacc cggtggtgat cggcatcaaa cagatgattt cgggcggaga tgcccagacc
     4561 gagtggaaca tcgtcatggc caccgccatg ctggccatgc tgccaccggc attggtggtg
     4621 atgctgatgc agaagtggtt tgtcaaaggc ctggtggaca cggaaaaata atggcttcca
     4681 tagaactcaa aaacgtcatc aagcgctacg gtgcaggcaa gggctccaac caggtcatcc
     4741 acggcgtgaa cgcgcagatt gccgacggag aattcgtcgt catcgtggga ccctccggct
     4801 gcggaaagtc cacgctgctg cgcatggtgg cagggctgga agaaatcagc agtggcgaaa
     4861 tctgcattgg cggccgggtg gtcaaccggc tcgaacccag cgaacgcgat atcgccatgg
     4921 tgttccagaa ctatgcgctc tacccgcaca tgaccgtgtt cgagaacatg gcttacgggc
     4981 tgaagattgc caagg
//
LOCUS       DAOSJM010000166         4965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15133_length_4965_cov_3.478411, whole genome shotgun sequence.
ACCESSION   DAOSJM010000166 DAOSJM010000000
VERSION     DAOSJM010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15133_length_4965_cov_3.478411"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..715)
                     /locus_tag="PKX82_07070"
     CDS             complement(56..715)
                     /locus_tag="PKX82_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOF52051.1"
                     /translation="MIKKIKYLFYLIVFIGFSSANAGSYEDFFIAIKQDNARVIQELI
                     FRGFDPNTPNPDGHYGLMLAIKEPSPRVAEALVAASETRVEVRNASDESPLMLASLKG
                     FRKLCELLIARDADVNKPGWTPLHYAATGGHTEVVQLLLDNHAYVDAASPNGSTPLMM
                     AAMYGSPAAVQLLLDAGADPALKNDLGLTAIDFARQGLKRDSAEIIATHIRRRSSTGA
                     W"
     gene            complement(712..1506)
                     /locus_tag="PKX82_07075"
     CDS             complement(712..1506)
                     /locus_tag="PKX82_07075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOF52052.1"
                     /translation="MFTDSHCHLTFPELASSIESIRSSMAQAQVTRALCICTTLEEFD
                     QVHGLALQYPNFWASVGVHPDNEDVMEPSLQDLVSRGSMDKVVAIGETGLDYYRLGER
                     TLADMEWQRERFQTHIRAARAIAKPLVIHTRSASGDTLDILRAEGENGSAGSAGGVFH
                     CFTETIEVAKAALDLGFYISFSGIVTFKSAQDIRDVAAMVPMDRMLIETDSPYLAPVP
                     FRGKTNNPSYVPHVAKQLALVKDCSVESIAQATSENFLKLFKLKIS"
     gene            complement(1507..1869)
                     /locus_tag="PKX82_07080"
     CDS             complement(1507..1869)
                     /locus_tag="PKX82_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HOF52053.1"
                     /translation="MTSTAASTPRPSVIQLSIKEKAALHAAYIPAFTDGGVFIPTARE
                     YALGDDVYVLLSLPDDIQRYPVAGKVAWVTPAKAAGGRTQGVGIVFPKDEKSRALRLK
                     IEEILGSQLASDRPTQTI"
     gene            complement(1881..2891)
                     /locus_tag="PKX82_07085"
     CDS             complement(1881..2891)
                     /locus_tag="PKX82_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOF52054.1"
                     /translation="MTTPSAEVVAPWIAAQTTHLLAQRGHAWLLHGPSGLGQYRLAMA
                     LARAWLCEHPTPAGACGQCGSCHAIDVRTHADLCVLMPETTMLELGWPLSEKAQSEID
                     DKKRKASREIRVDAMRDTVEFAQRTSARGRGKVVLVFPAEDMNAITANALLKTLEEPP
                     GDVRFVLATESAHQLLPTIRSRCIGHTLAWPDAEAAQAWLVSTGISGQDAALLLKATG
                     GRPEDALAYLESGRNPRDWALFPKAMARGDAGFVKDWSAPQLIDGLHKLCHDMLAVCT
                     GASPRFFQPGDLLPHAALAPLTAWSRALAKSMRTMDHPFNAGLQQEALVAQAQTALNS
                     TH"
     gene            complement(2888..3535)
                     /gene="tmk"
                     /locus_tag="PKX82_07090"
     CDS             complement(2888..3535)
                     /gene="tmk"
                     /locus_tag="PKX82_07090"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOF52055.1"
                     /translation="MNGLFISFEGIDGAGKSTHIDRLAQCFRDMGRQVTLTREPGGTP
                     LAERLRQMVLNDPMDAMTEALLVFAARRDHVQNVIVPSLLAGQVVLCDRFTDATFAYQ
                     GQGRGFDLAILNTLEQWVQGTGLDAALPQGLLQPALTVWFDLPAQVAAQRLATARVPD
                     KFEAQPQAFFEQVSQGYAMRCAAAPQRFERINANQPLESVWTDVEAALRGRGLLR"
     gene            complement(3540..4517)
                     /gene="mltG"
                     /locus_tag="PKX82_07095"
     CDS             complement(3540..4517)
                     /gene="mltG"
                     /locus_tag="PKX82_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOF52056.1"
                     /translation="MFFAVVLAAVLGTCAFAYRWVNAPLELATSPVDLSIESGSSARD
                     VANAAVEAGVPLNPMLLYWWFRLSGDARQIKAGSYELEQGLSPRTLLRKLVRGEEALR
                     SVTLVEGWTFRQVQDALRKADQLKPQTQGMSSDAIMQALEMPGVAAEGRFFPDTYTYA
                     KGSSDLAVLRRAAHAMDKKLAAAWAQRGPQSPLQTAEQALILASIVEKETGKGSDRAM
                     VSSVFNNRLRIGMRLQTDPTVIYGLGDSFDGNLRKKDLLTDTPWNTYTRVGLPPTPIA
                     MPGKASLQAAVQPAPSKALYFVARGDGSSEFSATLDEHNRAVNKYQRGQ"
     gene            4570..>4965
                     /locus_tag="PKX82_07100"
     CDS             4570..>4965
                     /locus_tag="PKX82_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52057.1"
                     /translation="MNAPHSESPADTLPSGVANLSHLGVIQISGEDAAKFIHGQLTQD
                     FSLLGQDQARLAAFCSAKGRMQASFVGFKRPNGDILLVCSRDLLPATLKRLSMFVLRA
                     KAKLTDASAQLALYGLAGDAIKKIADGAHS"
BASE COUNT         1032 a         1470 c         1418 g         1045 t
ORIGIN      
        1 tctgcactat agttcgaggt ctatatgact tatacatcgt ataaagtagg atgcattacc
       61 aggctcccgt ggaactcctg cgtcgaatat gcgtcgctat gatttctgcg gaatccctct
      121 taagcccctg cctggcgaag tcgattgcgg tcaacccgag atcgttcttc agtgccggat
      181 cagcacccgc atccaggagc aactgcactg ccgcaggcga accgtacatt gctgccatca
      241 tcaacggcgt gctgccgttg ggtgaagcag cgtccacgta cgcgtggtta tccagcagca
      301 attggacaac ttctgtatgt ccacccgtag cggcgtaatg caatggcgtc cagccaggct
      361 tgttgacgtc cgcatcgcgc gctatgagca gctcgcaaag ctttcggaat cccttgaggg
      421 aagccaacat caaggggctt tcgtctgagg cgttgcgaac ttcaacccgt gtctctgatg
      481 cggcaacgag agcttctgcc acacgcggtg acggctcttt aattgccaac atcagcccgt
      541 aatggccatc gggattgggt gtgttcgggt cgaagccacg aaaaatcaac tcttggatta
      601 ctctggcatt gtcttgtttg atcgcaatga agaagtcctc ataagatccg gcatttgcag
      661 aagaaaaccc aataaaaaca atgagataaa aaagatactt aatctttttt atcatgatat
      721 ttttagcttg aaaagcttta agaaattttc actggtagcc tgcgcaatcg actcaacaga
      781 gcagtctttc acaagagcca gctgtttcgc cacgtgaggc acatacgagg gattattggt
      841 tttgccgcgg aatgggaccg gagccagata cggactgtcg gtctctatca gcatgcgatc
      901 catgggaacc atggccgcca cgtcccggat atcctgcgcg gatttgaacg taacgatgcc
      961 tgaaaacgaa atgtagaacc ccaggtccag cgcagccttg gcaacttcga ttgtttcagt
     1021 aaaacaatga aacacaccgc ctgcagaccc ggcactcccg ttttcgccct cagccctcaa
     1081 gatgtccaac gtatcaccgg aggcgctccg ggtatggatc accagtggtt ttgcaatggc
     1141 gcgggccgcg cggatgtgtg tctggaagcg ttctcgctgc cactccatat ctgccaaagt
     1201 acgttcgccc aaacggtaat agtccagacc ggtttcacct atggctacaa ctttgtccat
     1261 cgagccgcgt gataccaagt cctgcaggct tggttccatg acatcttcgt tgtccggatg
     1321 tacgcccacg ctggcccaga aattgggata ctgcagggcc agcccgtgca cctggtcaaa
     1381 ttcctccagt gtggtgcaga tacacagcgc ccgggttacc tgggcttgcg ccatggaaga
     1441 ccggatggat tcaatagagg aagccagctc tggaaatgtc aggtggcagt gtgaatcggt
     1501 aaacactcaa atggtttgtg tgggacggtc ggatgcaagt tgcgagccga gaatttcttc
     1561 aattttcagc cgcagtgcac gggacttctc atccttgggg aaaacaatgc cgacaccctg
     1621 ggtccgtcca cctgccgctt tggcgggtgt aacccaagcc actttgcccg ccacaggata
     1681 acgctggatg tcatccggaa gcgacagcag cacatacacg tcatcaccca atgcgtattc
     1741 acgggcagtg ggaatgaaga cccccccatc cgtgaacgcg ggaatataag ccgcgtgcaa
     1801 tgcagctttt tccttgatgg aaagctggat gacactgggt ctgggtgtag aggctgcggt
     1861 gctggtcatg gtcgacagtt tcaatgcgtg gagtttaggg ctgtttgggc ctgtgcgaca
     1921 agcgcttcct gctgcagccc ggcattgaac gggtggtcca tggtgcgcat ggacttggcc
     1981 agtgccctgc tccaggccgt cagtggcgcc aacgcagcgt gtggtaacag atcacctggc
     2041 tggaaaaacc gtggagatgc acctgtgcac acggccagca tgtcatggca cagcttgtgc
     2101 agtccgtcga tcagttgcgg ggcagaccaa tccttgacaa aacctgcatc acctcgggcc
     2161 atcgccttgg gaaataaggc ccaatcccgc gggttgcggc ccgactccaa gtaagccaag
     2221 gcgtcttcag ggcgaccacc ggtggctttg agcagcaacg cagcatcctg tcctgaaatg
     2281 cctgtggaca ccaaccaagc ctgtgcggcc tccgcgtctg gccaggccag cgtatgacca
     2341 atgcagcggc tgcgaatggt gggcaacagc tggtgcgcgg attccgtggc cagcacaaac
     2401 ctgacatcgc caggaggctc ctcgagcgtc ttgagcagcg cattcgccgt gatggcgttc
     2461 atgtcttcag cgggaaacac cagcaccact tttccgcggc cccgcgcact ggtgcgctgg
     2521 gcaaactcca cggtgtcccg catggcatct acacggattt cccgactggc cttacgtttc
     2581 ttgtcatcaa tttcgctttg cgccttttcg ctcaggggcc accccagttc gagcatcgtg
     2641 gtctcaggca tcaggacgca caaatcagca tgtgtgcgca cgtcgatcgc atggcaactg
     2701 ccgcactgcc cgcaggcccc ggcaggcgtg gggtgctcgc aaagccaggc acgggccagc
     2761 gccatggcca accggtactg gcctaaccca gagggaccgt gcagcagcca cgcgtgaccg
     2821 cgttgggcca acagatgcgt ggtttgcgcc gcgatccagg gcgcaaccac ttcggccgat
     2881 ggtgtggtca tctgagtaat cccctgcccc gcaatgcagc ttccacgtcg gtccagacgg
     2941 actccagcgg ttggttggca ttgattcgtt caaaacgctg aggtgctgca gcacaccgca
     3001 tggcgtaccc ctgcgaaacc tgttcaaaga aggcctgggg ttgagcctca aatttgtctg
     3061 gcacacgggc tgtggccagc cgctgggcgg caacctgagc aggcagatcg aaccatacgg
     3121 tcaacgcggg ctgcagcaga ccctgcggca gggctgcatc cagtcctgtg ccctgaaccc
     3181 attgctcgag tgtgttgaga atggccaagt cgaagccgcg accctggccc tggtaggcaa
     3241 acgtggcatc cgtaaagcgg tcacacagca cgacctggcc ggccaacagg gagggaacaa
     3301 tcacgttttg aacatggtcg cgccgtgccg caaacaccag cagggcttcg gtcatggcgt
     3361 ccatcgggtc gttcagcacc atctggcgca gtcgctccgc cagtggcgta ccaccaggtt
     3421 cgcgggtgag ggtcacttgc cgccccatgt cccggaagca ctgcgcaagt ctgtcaatgt
     3481 gtgtggactt gcccgcaccg tcgatgccct cgaaactgat aaagagtccg ttcatcggtc
     3541 tattgtccac gctggtattt gttgacggcc cggttgtgct cgtccagggt ggcactgaat
     3601 tcgctactgc catcaccccg tgccacaaag tacagggcct tggaaggtgc gggttgtacc
     3661 gcggcttgca gcgaagcttt gccgggcatc gcaatcggcg tgggtggcaa cccgacgcgg
     3721 gtataggtgt tccagggggt gtctgtcagc aaatctttct tgcgcagatt gccatcgaac
     3781 gaatcgccca ggccataaat cactgtgggg tcggtttgca gacgcatacc gatccgcaag
     3841 cggttgttga acaccgatga caccatggcc cggtcgctgc cctttcccgt ttccttttcg
     3901 acaatgctgg caagaatgag cgcctgttcc gctgtctgca gcggtgattg ggggccgcgt
     3961 tgtgcccagg ccgcggccag cttcttgtcc attgcgtggg ccgcgcgcct cagcaccgca
     4021 aggtcgctac tgcctttggc gtaagtgtag gtatccggaa aaaagcgccc ttcggcggcc
     4081 acaccgggca tctccagcgc ttgcataatg gcgtcgctgg acatcccctg tgtctgcggc
     4141 ttgagttggt cggctttgcg cagggcgtct tgcacctgcc ggaatgtcca gccttccacc
     4201 agcgtgacgc tgcgtagggc ttcttcgccg cgtaccagtt tgcgcaacag ggtccgcggg
     4261 ctcaggccct gctccagctc gtaactgccc gccttgatct ggcgggcatc cccagacagc
     4321 cggaaccacc agtacagcag catcgggttc aagggcacac cggcttccac cgccgcgttg
     4381 gccacatccc gtgcactgga tccggactcg atggacaaat ctacaggcga agtggccagt
     4441 tccaggggcg cattgaccca gcgatacgca aaggcgcatg tccccaggac ggcagccagc
     4501 accacggcaa agaagatacg cacagcccta cattcctcgt gaattcatag gggaatcata
     4561 attgacgcca tgaacgcacc acattccgaa tctcctgccg acactctgcc tagcggggtt
     4621 gcgaacctgt cccatctggg cgtcatacag atcagtggtg aggacgccgc caaattcatc
     4681 cacgggcagc tgacacagga cttctccctg ctgggacaag accaggcacg actggccgcg
     4741 ttctgttccg ccaagggccg catgcaggcc agttttgttg ggttcaagcg ccccaatggc
     4801 gacatccttc tggtctgcag ccgtgatctg ctgccggcga cgctcaagcg cctgtccatg
     4861 tttgtgctgc gcgccaaggc caaactcacc gatgcgtctg cacagttagc gttgtatggt
     4921 cttgcgggag atgctattaa aaaaatagct gatggcgccc attcc
//
LOCUS       DAOSJM010000167         4961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15146_length_4961_cov_4.346311, whole genome shotgun sequence.
ACCESSION   DAOSJM010000167 DAOSJM010000000
VERSION     DAOSJM010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4961
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15146_length_4961_cov_4.346311"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1669
                     /locus_tag="PKX82_07105"
     CDS             <1..1669
                     /locus_tag="PKX82_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF52058.1"
                     /translation="AYLQTLDILEEGVVEIAADGSYLRASPGWTRLAGTARSHGGSLF
                     ESFHPDDVGDFKLHFAQLFSGEKTSLRGRIRLRRTDGKDVWVEYRFVVSELGAMAVRS
                     VRGVLRDITQSYLMEKHVTHMALHDALTGLPNRVLLEDRCKIALRSAERTGSRVALGF
                     MDLDHFKHVNDQFGHKAGDEMLLYLSNALRGCLRSGDTLARWGGDEFVVLLTDLTGQE
                     GAREAIAKMVSTCQQPIMVDGNVFNSTFSMGVAMYPDDASTVDALLSQADRAMFSAKE
                     QGRNTIRFFSDITNVDEDRKSVYIQQRLAAAIKDHKIQTWFQPIVDVHTRRVVSCEAL
                     ARWYDESYGWVSPATFIPMAESMGLIREVGQQVWLQAVEALHRWREAGMDMRISVNVS
                     RRQLFTPTFTADLLADLDRLAIPVSVIDLEITESVAMEDAEFTAKRLLELTEAGFGIA
                     IDDFGTGYSSLSQLHDMPATKIKIDISFVRRAHSGQGAQLIQAIVNIAGAFRLKTVAE
                     GVESEDIADALQGFGVDLLQGYHFGKPMPLAEFEHFLSSRFIGNSKL"
     gene            complement(1666..2997)
                     /gene="fahA"
                     /locus_tag="PKX82_07110"
     CDS             complement(1666..2997)
                     /gene="fahA"
                     /locus_tag="PKX82_07110"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HOF52059.1"
                     /translation="MPTLNPTHDPQATSWVASAQATGADFPIQNLPLAVFRRAGSHAA
                     FRGGVAIGDQIIDLAAAQAAGVLDGLGAQALAAAAEPTLNHFFAEGPSAWRALRHGLF
                     DLLHSSATGPKVDALRTCLVAQFDAEFTLPARVANYTDFYTSIHHARNVGKVIRPDDP
                     LTPNFQWLPIAYHGRASSVVMSGTAFHRPHGQAMPPGARMPVYGACARLDYELEMGFF
                     IGTGNALGEPVPLGEAEDHIFGMCLLNDWSARDHQFWEMNPLGPFLGKNFCTSISPWI
                     VTMEALAPYRVPAVERPAPEPQPLAYLDGAANRAAGGVDVQLTVGIESAEHRARGLAP
                     AEVSRTNFHHQYWTIAQMVTQHTVGGCNLQPGDLLGTGTISGPTPAEAGAMVELSRGG
                     TQAITLAGTGETRTFLQDGDTVVLRGWCEKSGAARVGFGECRGQVLPARAR"
     gene            complement(3068..3874)
                     /locus_tag="PKX82_07115"
     CDS             complement(3068..3874)
                     /locus_tag="PKX82_07115"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOF52060.1"
                     /translation="MARTFVDLSIFLENDVLSDPPAFAPKIEYFTHENTFAQIEPFFP
                     GLKKEDLPDGEGWAVEMVQLSTHNGTHLDAPYHFHSTMDAKNGEKKPAIAIHEVPLEW
                     CFQPGVKLDFRHFADGYVVTAADVEAELQRIGHTLTPLEIVVVNTRAGSRYGHPDYVA
                     AGCGMGYEATMYLLERGVRLTGTDAWSWDAPFVHTAQKYGASLDASLIWEGHKAGRDI
                     GYCHLEKLHNLEALPPTGFFISCFPHKIRGASAGWTRAVAIFDDALMASR"
     gene            complement(3903..4685)
                     /locus_tag="PKX82_07120"
     CDS             complement(3903..4685)
                     /locus_tag="PKX82_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF52061.1"
                     /translation="MSSAVLQSSTRGRATTESVAVLVCLLALWWLASHLQWVSKVFLP
                     TPEATLESLVQGLSTGELAGFTWDTVVRMLLGWLLASAIGTALGALIGASATARTWIA
                     PTLEFLRPLPASAVMPLAISLFGLSGGMVLSVVAFGAMWPVLLATVHGLASVHVRLRE
                     VATALQLSRTAFVFKIGLPNALPDILAGMRLSMTVSLIVAVVGEMIASQSGLGQAILL
                     AARSFRASELFAGIVLLGAIGFVSNALLALLEHQFLKWQRTG"
     gene            complement(4682..>4961)
                     /locus_tag="PKX82_07125"
     CDS             complement(4682..>4961)
                     /locus_tag="PKX82_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF52062.1"
                     /translation="QIVLPAMVPRLFIALRLGVSFALVVAVTVEIAANPSGMGYAMMI
                     AQQSLAPDLMLAWLAWIGVVGFAINALTLRLQRAIAHRMGEPRTGVAP"
BASE COUNT          878 a         1555 c         1674 g          854 t
ORIGIN      
        1 tgcctacctg caaacgctcg atatcctgga agaaggggtg gtggaaattg cggccgacgg
       61 cagctacctg cgcgccagtc cgggctggac gcgcctggcg gggaccgcac gcagccacgg
      121 cggcagtctg ttcgagagct tccatcccga tgatgtgggc gatttcaagt tgcactttgc
      181 gcagctgttc tccggcgaaa agaccagcct gcgtggccgc atccgcttgc ggcgtaccga
      241 tggcaaggac gtgtgggtgg aataccgctt tgtggtcagc gagctcggtg ccatggcggt
      301 gcgcagtgtg cgtggtgtgc tgcgcgacat cacgcaaagc tacctgatgg aaaagcacgt
      361 gacgcacatg gccctgcacg atgcgctcac cggcctgccc aaccgcgtgc tgctggagga
      421 ccgctgcaag attgccctgc gcagtgccga gcgcaccgga agccgggtgg cactgggttt
      481 catggacctg gaccatttca agcatgtcaa cgaccagttt ggccacaagg cgggggacga
      541 gatgctgctg tacctgtcca acgcactgcg cggctgcctg cgctcgggcg acacgctggc
      601 gcgctggggt ggcgacgagt tcgtcgtgct gctgaccgac ctgaccgggc aagagggcgc
      661 acgcgaggcc attgccaaga tggtgtccac ctgccagcag cccatcatgg tggacggcaa
      721 tgtcttcaat tccaccttca gcatgggcgt ggccatgtac cccgacgatg ccagcaccgt
      781 cgatgccctg ctgtcccagg ccgaccgcgc catgttctcg gccaaggagc aggggcgcaa
      841 caccatccgc tttttttccg acatcaccaa tgtggacgaa gaccgcaaat cggtctatat
      901 ccagcagcgg ctggccgcgg ccatcaagga ccacaagatc cagacctggt tccagcccat
      961 cgtcgatgtg cacacccgcc gcgtggtgag ttgcgaggcg ctggcccgct ggtacgacga
     1021 aagctacggc tgggtgtcgc ctgccacgtt catcccgatg gccgagagca tgggcctgat
     1081 ccgcgaagtg ggccagcagg tctggctcca ggcggtggag gccctgcacc gctggcgcga
     1141 ggcgggcatg gacatgcgca tctcggtcaa tgtgtcgcgc cgccagctgt ttacccccac
     1201 ctttaccgcg gatctgctgg ccgacctgga ccggttggcg ataccggtgt cggtgatcga
     1261 cctggagatc accgagagtg tggccatgga agacgccgag ttcaccgcca agcggctgct
     1321 ggagttgacc gaagccggtt ttggcatcgc gattgacgac tttggtaccg gctactcctc
     1381 gctttcgcag ttgcacgaca tgccggccac caagatcaag atcgatattt cgtttgtgcg
     1441 ccgtgcccac agtgggcagg gggcgcagct gatccaggcg attgtcaata ttgccggcgc
     1501 cttccggctc aagaccgtgg ccgaaggggt ggagagcgag gacatcgccg atgccctaca
     1561 gggctttggt gtggacctgc tgcagggtta ccactttggc aagcccatgc cgctggccga
     1621 gttcgagcac tttctctcca gccgcttcat cggcaacagc aagctctagc gggccctggc
     1681 gggcagcacc tggccgcggc attcgccaaa gcccacgcgt gctgcgccgg atttctcgca
     1741 ccagccgcgc aggaccacgg tgtcgccgtc ctgcaggaag gtgcgtgtct cgccggtgcc
     1801 tgccagcgtg atcgcttgcg tgccgccccg gctcagttcc accatggccc cggcctcggc
     1861 cggtgtgggg ccagaaatcg tgccggtgcc cagcaggtcg ccgggctgca ggttgcaacc
     1921 gcccaccgtg tgctgggtga ccatctgggc gatggtccag tactggtggt ggaaattggt
     1981 gcgtgagact tccgcgggtg ccaggccacg tgcgcggtgt tccgccgatt caatgcccac
     2041 ggtcagctgc acatccacac cgcccgctgc gcggttggcc gcgccgtcca ggtaggccag
     2101 gggctgcggt tcgggtgcag ggcgttccac ggcgggcacc cggtagggcg ccagcgcttc
     2161 catggtgacg atccacggcg aaatgctggt gcagaagttc ttgcccagga atgggcctag
     2221 tgggttcatc tcccagaact ggtggtcgcg tgccgaccag tcgttgagca ggcacatgcc
     2281 gaagatgtgg tcctcggctt cgcccagagg tacaggttcg cccagcgcat tgccggtgcc
     2341 gatgaagaag cccatttcca gctcgtagtc caggcgcgcg caggcgccgt acacaggcat
     2401 cctggcgccc ggtggcatgg cctgtccgtg cgggcggtga aacgcggtgc cgctcatcac
     2461 cacgctcgat gcccggccat ggtaggcaat gggcagccac tggaagttgg gggtcagcgg
     2521 atcgtccggg cggatgactt tgccgacgtt acgggcatgg tggatggagg tgtagaagtc
     2581 ggtgtagttg gccacgcgcg cgggcagcgt gaattcggcg tcaaactgcg ccaccaggca
     2641 ggtgcgcagg gcgtccacct tcgggccagt ggcgctgctg tgcagcaggt cgaacaggcc
     2701 gtggcgcagc gcccgccagg cgctggggcc ttcggcaaag aaatggttga gcgtgggttc
     2761 ggccgctgcc gccagggcct gtgcacccag gccgtcgagc acaccggccg cctgtgctgc
     2821 agccaggtcg atgatctggt cgccaatggc cacaccgccg cgaaacgccg cgtggctgcc
     2881 ggcgcgccgg aacaccgcca gcggcagatt ctggatgggg aagtcggcgc cggtggcctg
     2941 ggcggaagcc acccagctgg tggcctgcgg gtcgtgggtg ggattcagtg tgggcatgat
     3001 tgctcctgat ttaatagcgc actacgccca ttcaacgagg gctagaggcc aatttgattg
     3061 acaaatctca gcgggatgcc atcagcgcgt cgtcaaaaat ggccaccgcg cgggtccagc
     3121 cggccgaggc gccgcggatc ttgtgcggaa agcagctgat gaaaaaaccg gtcggcggca
     3181 gggcttccag gttgtgcagt ttttcaaggt ggcagtagcc gatgtcgcgc ccggccttgt
     3241 ggccttccca gatcagcgag gcgtcgaggc ttgcgccgta cttctgggcg gtgtgcacaa
     3301 agggggcgtc ccagctccag gcgtcggtgc cggtcaggcg tacgccgcgc tccagcaggt
     3361 acatggtggc ttcgtagccc atgccgcagc cggcggccac gtaatcgggg tggccgtagc
     3421 gcgatccggc ccgcgtgttg accaccacga tttccagtgg ggtcaatgtg tggccaatgc
     3481 gctgaagctc cgcctcgacg tcggcggcgg tgactacgta gccgtcggca aagtggcgga
     3541 agtccagctt cacgccgggc tggaaacacc attcgagcgg aacttcgtgg atggcgatgg
     3601 cgggtttctt ttcgccattc tttgcgtcca tggtgctgtg aaaatggtag ggcgcatcca
     3661 ggtgggtgcc gttgtgggtg gagagctgca ccatctccac cgcccagccc tcgccatcgg
     3721 gcaggtcttc tttcttcaga ccggggaaga agggttcgat ttgcgcaaag gtgttttcgt
     3781 gcgtgaagta ctcgatcttg ggtgcaaaag ctggcggatc gctcagcaca tcgttttcca
     3841 gaaagatgga aagatctaca aaagttcggg ccatggtcgc tcctagggat ggggtggcag
     3901 cgtcatccgg tgcgctgcca tttcagaaac tggtgttcca gcagggccag cagcgcattg
     3961 ctgacaaagc cgatcgcgcc cagcagcacg atgccggcaa acagctcgct ggcgcgaaac
     4021 gaccgggccg ccagcaggat ggcctggccg agtccggact gcgaggcaat catttcgccg
     4081 accacggcca cgatgagcga gaccgtcatc gacaggcgca tgcccgccag gatgtcgggc
     4141 agggcgttgg gcaggccgat cttgaataca aaggctgtgc gtgacagctg cagcgcggtc
     4201 gccacctcgc gcaggcgcac atggaccgat gccaggccgt gcacggtggc cagcagcacc
     4261 ggccacatgg caccaaatgc caccacggac agcaccatgc cgccactcag cccaaagagc
     4321 gaaatcgcca ggggcatgac cgccgatgcc ggcagcgggc gcagaaattc cagcgtcggc
     4381 gcaatccagg tgcgtgcggt ggcggatgcg ccaatcagcg cacccagcgc ggtcccgatc
     4441 gccgaggcca gcagccagcc cagcagcatg cgtaccaccg tgtcccaggt aaacccggcc
     4501 agttcgccgg tggacaggcc ctgcaccaga ctctccagcg tggcctcggg agtcggcaga
     4561 aacaccttgc tcacccactg caggtggctg gccagccacc acagcgccag caggcaaacc
     4621 agtacggcca ccgactcggt agtggcgcgg ccgcgagtgc tgctctggag cacggcgctg
     4681 ctcatggcgc gactccggtg cgcggttcgc ccatgcggtg ggcgatggcg cgctgcagcc
     4741 gcagggtgag cgcgttgatg gcaaagccca ccacgccaat ccaggccagc caggccagca
     4801 tcaggtccgg tgccagactc tgctgcgcaa tcatcatggc gtagcccatg ccactggggt
     4861 tggcagcgat ctccacggtg acggccacca ccagcgcaaa cgacacaccc aggcgcagcg
     4921 caatgaacag ccgcggcacc atggccggta gcacgatttg c
//
LOCUS       DAOSJM010000168         4955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15165_length_4955_cov_3.439592, whole genome shotgun sequence.
ACCESSION   DAOSJM010000168 DAOSJM010000000
VERSION     DAOSJM010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15165_length_4955_cov_3.439592"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..1082)
                     /locus_tag="PKX82_07130"
     CDS             complement(30..1082)
                     /locus_tag="PKX82_07130"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOF52063.1"
                     /translation="MNTKATSSSSASDIDRELDQALASGPLRDIVIPPCPELLVALRM
                     EMGQADPDPLVIADIAGRDVAMAALLIRTANSPYYARSRPVTSVPEALGLLGVRASEK
                     LLTTFLVRFAIRINSPLIEHFWETSTRRAIAMGYIAKQLYGVDADVAHTCGLFCHVGI
                     PVMLQGVKGYSGTLAEALARQDRTFTETENAAHRTDHAVVGAIVARTWRLPPIISVAV
                     RLHHDFTVLKADSVPQEARTLVAMALVAEYLVGQHEGVREQKEWTRHGDECLAFLNIN
                     RDELDSWVANLHPMLERYCGLSSWASRPRLRIFCTVAVASSRVLNGPTRTRVKVPRGP
                     VTSWLNAARPAAVRVS"
     gene            complement(1144..3231)
                     /locus_tag="PKX82_07135"
     CDS             complement(1144..3231)
                     /locus_tag="PKX82_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF52064.1"
                     /translation="MSDDAFLDRVLGHASHLLRGLARNDIPGLFVIQDGTYLYVNPRF
                     AEMFGYTQAEVCDGTVGPRTLVAPEDWARVQAGIAERLSGRVLTGHYDFVGLKKDGTR
                     FEVELFGTTTEIDGKPAIIGLLVDISVRFRAERKLQDQLRFVEQLIDTIPGPLSYKDE
                     QGRYLGCNRAFEAMVGKSRDEFLGHTVHSITYRKVAEKMHTADQRLINNPGIEIYESQ
                     IPHIDGTTRDMMLYKATFNKADGSVGGLVTLMLDISERKLMEQRIWEEANYDSLTGLP
                     NRRMFRNRLDEEIKRSRRSGLSCALLLVDLDRFKEVNDTLGHEMGDHLLVEAAKRITA
                     CVRESDSVARLGGDEFMVILPEITDPAGIGQVATHIIDALVKPFQLRGHAAYVSASVG
                     VALFPEDAGDIDTLVSYADQAMYAAKEQGRSCFSYFTPALQERSRFRLEVANELRGAL
                     AANQLEVYYQPIIDLASGQVIKAEALLRWTHPTMGMVPPDRFIPIAEEVGLIHTIGDW
                     VFQQAASAARRWQVEFGRDIQISVNKSPRQLVTGRTQETWIDYLRDIGLAPGLVCIEI
                     TEGLLMDSRPEVASKLLLFRDAGIQVSLDDFGTGYSAMSYLKKFDIDFLKIDKSFVRD
                     LTTDTTDRAIAEAIIAMAHKLGLQVIAEGVETQEQRALLSDAGCNFAQGYLFARPMPL
                     AAFEDFLCAADVQ"
     gene            complement(3322..4281)
                     /locus_tag="PKX82_07140"
     CDS             complement(3322..4281)
                     /locus_tag="PKX82_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOF52065.1"
                     /translation="MLSRTADHLFWMSRYTERAENTARMLDVNYQTSLLPQSETAAQV
                     GWQGLLSISELLFTYKERYGEIQAREVMDFMVKDESNPSSILSCLGAARENARAVRGT
                     LTTEVWETQNQTWLEVKRMIKSGEFEQDPAQFFEWVKFRSHLSRGVTVGTMLMDEALF
                     FMRMGTFLERSDNTARLVDVKFHAVQSDFFGTGKEKDQEYDFYHWSAILRSVSGFEVY
                     RKVYRDVIRPERVAELLILRPDMPRSLHASLNEVVNNLALVASNPGSETLRRAGKLRS
                     ELQYGRIDEILATGLHAYLTQFLDRVNDLGAHISREFLVTGAA"
     gene            complement(4295..>4955)
                     /locus_tag="PKX82_07145"
     CDS             complement(4295..>4955)
                     /locus_tag="PKX82_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOF52066.1"
                     /translation="DNFVYMRTTRGPRRVDVIYRRVDDDFLDPQVFRPTSTLGCAGLM
                     SAYKAGNVTICNAVGTGVADDKSIYPYVPKMIEFYLGEKPILNNVPTYMCRNKDELSY
                     VLANLKDLVVKEVHGAGGYGMLVGPAATKAEIEDFRKAVEANPSGYIAQPTLSLSSCP
                     TFVESGIAPRHIDLRPYVLSGKTVQMVPGGLTRVALKDGSLVVNSSQGGGTKDTWILE
                     V"
BASE COUNT          959 a         1572 c         1472 g          952 t
ORIGIN      
        1 tttgccgatg ccggtaacgc gcgcagggtt caggacaccc tgaccgcagc cggcctggca
       61 gcattcagcc aggaagtcac cgggccgcgt ggaactttca cccgcgtgcg ggtgggtcca
      121 ttcaaaaccc gcgaagaagc gacggccacg gtgcagaaga tacgcaatct gggtctggag
      181 gcccagctac tcaacccgca ataacgctct aacatggggt gcaggttggc cacccagcta
      241 tcgagttcat cccggttgat gttgagaaag gccaggcact cgtctccatg gcgggtccat
      301 tccttttgct cacgcacccc ttcgtgctgg cccaccaggt actccgccac cagcgccatg
      361 gccacgagcg tgcgggcctc ctgcggaacg ctgtcagcct tgagcacggt gaagtcgtgg
      421 tgcaaccgca ccgccaccga gatgatgggg ggcaaccgcc aggttcgggc cacgatggcc
      481 cccaccaccg catggtcggt acggtgcgca gcgttttcgg tttcggtaaa cgtgcggtcc
      541 tggcgcgcca gcgcttcggc cagcgtgccg gaatagccct tgaccccctg cagcatcacc
      601 ggaatgccga catggcagaa caggccgcag gtatgcgcca catccgcatc cacgccatac
      661 agctgcttgg cgatgtagcc catggcgatc gcgcgccgtg tggaggtttc ccagaagtgt
      721 tctatcaagg gcgagttgat gcggatggca aagcgcacca gaaacgtggt gagcagcttt
      781 tctgaagcgc gcacccccag caaacccagt gcctcgggca ccgaggtgac aggccgcgaa
      841 cgtgcgtaat acgggctatt ggcggtacgg atcaacaatg ccgccatggc gacgtcccgc
      901 ccggcgatgt ctgcgatgac cagcgggtcc gggtcggcct ggcccatttc catacgcagg
      961 gccaccagca attcggggca cgggggaatg acgatatcgc gcagggggcc agaggccagc
     1021 gcctggtcga gctcgcggtc tatatcgctg gcggagctgc tggaggttgc tttggtattc
     1081 atgccattcc ggttgaattg gggctatgca ccattattga cgcgacggga ccgggtgcag
     1141 gggtcactgc acatctgcgg cacataaaaa atcctcaaaa gcggccagcg gcatcggccg
     1201 cgcaaacaga tacccctgcg caaagttgca tccggcatcg gacagcaacg cacgctgctc
     1261 ctgcgtttcc acaccctctg caatgacctg caggccaagc ttgtgggcca tggcgatgat
     1321 ggcctcggca atcgcccggt cggtggtgtc ggtggtcaga tcccgcacaa aggatttgtc
     1381 gatcttcaga aagtcgatgt caaatttttt cagatacgac atggccgaat acccggtacc
     1441 aaaatcgtcc aggctgacct ggattccggc atcgcgaaac agcagcagct tgctggcaac
     1501 ttccgggcgg ctgtccatca gcaagccctc ggtaatttcg atgcacacca gcccaggcgc
     1561 aagtccaatg tcccgcaggt aatcgatcca ggtttcctgc gtgcgtcccg tcaccagctg
     1621 ccgcggcgat ttgttcacgg aaatctggat gtcgcggcca aactcgacct gccaccgcct
     1681 ggccgcactg gccgcctgct ggaagaccca gtcacctatc gtatggatca ggcccacttc
     1741 ctctgcaatc ggaatgaacc ggtccggcgg caccatgccc atggtcggat gcgtccagcg
     1801 caacagcgcc tctgccttga ttacctgacc actggccagg tcgatgatgg gctggtaata
     1861 cacctccagc tggttggctg ccagagcacc gcgcagttcg ttggccacct ccagccggaa
     1921 ccgcgaacgc tcctgcaacg cgggcgtgaa atagctgaag cacgagcgcc cctgttcctt
     1981 ggccgcgtac atcgcctggt cggcgtagga caccaaggta tcaatgtccc ccgcatcctc
     2041 ggggaacagg gcaacaccca cgctagccga cacataggcc gcatggcccc gcagctggaa
     2101 tggcttcacc agcgcgtcta tgatgtgagt ggccacctgg ccgatacccg ccggatcggt
     2161 gatctccggc aggatgacca tgaactcgtc accgcccaga cgcgccacgc tgtcggattc
     2221 gcgcacgcag gctgtgatcc gtttggcggc ctccaccagt agatggtcgc ccatctcgtg
     2281 acccagggtg tcgttgactt ccttgaaccg gtccaggtcc accagcaaca gggcacagct
     2341 caggccgctg cggcggctgc gcttgatttc ttcatcgagc cggttgcgga acatgcggcg
     2401 attgggcaga cccgtcaggg agtcgtagtt ggcttcctcc catatgcgct gctccatcag
     2461 cttgcgctcg gaaatgtcca gcatgagcgt caccagacca cccaccgaac cgtcggcctt
     2521 gttgaaggtg gccttgtaca gcatcatgtc gcgcgtcgtg ccatcgatat gcggaatctg
     2581 cgactcgtag atttcaatac cggggttgtt gatcagtcgc tggtcggctg tgtgcatctt
     2641 ttcggccacc ttgcggtagg tgatgctgtg caccgtgtgc cccagaaatt cgtcacggga
     2701 tttgcccacc atcgcttcaa acgccctgtt gcagcccagg taacgcccct gctcatcctt
     2761 gtaggacaga ggccccggaa tggtgtcgat cagttgctcg acaaaccgca actggtcctg
     2821 cagcttgcgc tcggcacgaa agcgcacact gatgtccacc agcagtccga tgatcgcggg
     2881 cttgccatcg atctcggtcg ttgtgccaaa cagctccacc tcaaaacgtg tgccgtcttt
     2941 cttgagaccc acaaaatcgt aatgccccgt aagcacgcgc ccactgagcc gctcggcaat
     3001 cccggcctgc acgcgcgccc agtcctcggg ggccacaagc gttctggggc ccaccgtacc
     3061 gtcgcaaacc tcggcctgcg tgtagccaaa catctcggca aagcgcggat tgacgtacag
     3121 gtaggtgccg tcctgaatga caaacaggcc aggaatatcg ttcctggcca gaccgcgcaa
     3181 caggtgactg gcgtgcccaa gcacgcggtc taagaacgcg tcgtcggaca tcagtggggg
     3241 ctggaattca tggtgtgggc aaatgatacc ccccatgcat ccggcacctg gaccgcaagg
     3301 tcacccccgt tacggggcca ttcaggcggc gccggtgacc aggaattcgc ggctgatatg
     3361 ggcacccagg tcgttcacac ggtccaggaa ctgcgtcagg taggcatgca gaccggtggc
     3421 cagaatttca tcgatgcgac cgtactgcag ttccgagcgc agcttgcccg cccgtcgcaa
     3481 tgtctcactg cccggattgc tggccaccag cgccaggttg ttcaccacct cgttgaggct
     3541 ggcatgcagt gagcgtggca tgtcggggcg caggatgagc agctcggcga cccgctcggg
     3601 gcggatcaca tcgcggtaca ccttgcggta cacctcgaag ccagagacgc tgcgcagaat
     3661 cgcgctccag tggtagaagt cgtactcctg gtccttctcc ttgccggtac caaaaaagtc
     3721 gctctgtacc gcgtggaatt tcacatcgac cagacgggcg gtgttgtcgg aacgttccag
     3781 gaaggttccc atacgcatga agaacagggc ttcatccatg agcatggtgc ccacggtgac
     3841 gccgcgtgag aggtgcgagc ggaacttcac ccactcgaaa aactgcgccg ggtcctgctc
     3901 gaactccccg ctcttgatca tgcgtttgac ctccagccag gtctggttct gcgtctccca
     3961 gacttcggtg gtcagggttc cccgcacggc gcgggcgttt tcacgggcag cacccaggca
     4021 ggacaggatg gaagacggat tgctctcgtc cttcaccatg aagtccatca cttcacgtgc
     4081 ctggatctcg ccatagcgct ccttgtaggt gaacagcagt tcgctgatgg acagcagtcc
     4141 ctgccagccg acctgggcgg cggtttcgga ctggggcagc agcgaggtct ggtaattcac
     4201 atccagcatg cgcgcggtgt tttcggcccg ctcggtgtag cgggacatcc agaagaggtg
     4261 gtcagcggtt cttgacagca tatttttctc tcgatcaaac ttccagaatc caggtgtcct
     4321 tggtgccgcc gccctggctg ctattcacca ccagcgaccc gtccttcaag gccacgcgcg
     4381 tcaggccacc gggcaccatc tgcaccgtct tgcccgacag cacatagggc cgcaaatcga
     4441 tatggcgcgg cgcaatgccg ctttccacaa acgtggggca gctcgacaaa ctgagcgtag
     4501 gctgggcaat gtagccactg gggttggcct ccaccgcttt gcggaagtct tcaatctcgg
     4561 ctttggtcgc agccggcccc accagcatgc cgtagccgcc ggcaccgtgc acctccttga
     4621 ccaccaggtc cttgaggttg gccagcacat aggacagctc gtccttgttg cggcacatgt
     4681 aggtgggcac gttgttgagg atgggctttt cgcccagata gaactcgatc atcttgggca
     4741 cgtaggggta gatggacttg tcgtccgcca cacccgtgcc caccgcgttg cagatggtca
     4801 cgttgccagc cttgtaggcg ctcatcagtc ccgcacagcc cagggtggat gtggggcgga
     4861 acacctgcgg atccagaaag tcatcgtcca cacggcgata gatcacatcc acgcggcgcg
     4921 ggccgcgggt ggtgcgcatg tagacaaagt tgtcc
//
LOCUS       DAOSJM010000169         4941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15222_length_4941_cov_4.575727, whole genome shotgun sequence.
ACCESSION   DAOSJM010000169 DAOSJM010000000
VERSION     DAOSJM010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15222_length_4941_cov_4.575727"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="PKX82_07150"
     CDS             <1..964
                     /locus_tag="PKX82_07150"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOF52067.1"
                     /translation="GAAHFYQSKGKHIITVKTEHKAVLDTCRELERQGFEVTYLDVQE
                     DGLLNLESLKAAIRPDTILASVMFVNNEIGVIQDIAAIGAICREKGVIFHVDAAQATG
                     RVPFDLSQLPVDLMSLTAHKTYGPKGIGALFVRRKPRIRLEAQMHGGGHERGMRSGTL
                     PTHQIVGMGEAYRIAKEEMAVENVRIQALHDRMLAGLKDIEQVFINGHVDKRVPHNLN
                     MSFNYVEGESLIMGIKGLAVSSGSACTSASLEPSYVLRALGRSDELAHSSLRMTIGRW
                     TTEADIDYAVDTIKKNVAKLRELSPLWDMYKDGIDISTIQWAAH"
     gene            987..1373
                     /gene="iscU"
                     /locus_tag="PKX82_07155"
     CDS             987..1373
                     /gene="iscU"
                     /locus_tag="PKX82_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOF52068.1"
                     /translation="MAYSEKVVDHYENPRNVGSFEKGDDTVGTGMVGAPACGDVMKLQ
                     IKVNPQTGVIEDARFKTYGCGSAIASSSLVTEWVKGKTLDEAAALKNSQIAEELALPP
                     VKIHCSILAEDAIKAAVDDYKKKHLN"
     gene            1375..1698
                     /gene="iscA"
                     /locus_tag="PKX82_07160"
     CDS             1375..1698
                     /gene="iscA"
                     /locus_tag="PKX82_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HOF52069.1"
                     /translation="MAVTLTEAAARHVTRYLTKRGKGVGVRLGVKTTGCSGLAYQLEY
                     VDELSPEDVVFEGHGVKVLVDPKSLAYIDGTQLDFVREGLNEGFRFNNPNERDKCGCG
                     ESFRV"
     gene            1695..2210
                     /gene="hscB"
                     /locus_tag="PKX82_07165"
     CDS             1695..2210
                     /gene="hscB"
                     /locus_tag="PKX82_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOF52070.1"
                     /translation="MNRQADDFELFGLAQQFAQDRMVLDARWKELQREAHPDKFAAQG
                     AASQRVAMQWSVRINEAYQRLKDPLRRAAYLCELHGAAINAHSNTAMPASFLMQQMEW
                     REALEDASSAAALEALSDEVQAARLQLLQQCEALLDGQQAYAQAVEPVRALMFIEKFT
                     QDVNQRFEQFE"
     gene            2219..4075
                     /gene="hscA"
                     /locus_tag="PKX82_07170"
     CDS             2219..4075
                     /gene="hscA"
                     /locus_tag="PKX82_07170"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOF52071.1"
                     /translation="MALLQISEPGQSPNPHERRIAVGIDLGTTHSLVAAVRNGSAECL
                     PDTQGRVLLPSVVRYIDAQRREIGFDALAHQTTDPGNTIASVKRLMGRGAADIAHAEK
                     LPYALVDAPGMAQIRTIAGTKSPVEVSAEILATLRFRAEDTFNDDIYGAVITVPAYFD
                     DAQRQATKDAAQLAGLNVLRLINEPTAAAIAYGLDNAAEGVYAIYDLGGGTFDISILR
                     LSAGVFEVLATGGDSALGGDDYDRALAEWLLAESDVLAQTPEDQALVMAQARLCKEAL
                     SSEDIVTAEVDLSSGSIDLSLNREQFEAATAALTARTLQAVRKVLRDAALGKDDVKGV
                     VMVGGSTRMPQVQRAVAEFFGQPPLTNLNPDEVVALGAAVQANQLAGNNPSGDLLLLD
                     VIPLSLGIETMGGLVERIVPRNQTIPTAMAQDFTTYKDGQTALALHVVQGERDLVADC
                     RSLARFELRGIPPMAAGAARIRVTFTVDADGLLNVSAKEQVSGVEARIDVKPSYGLSD
                     DQIAKMLSESFTTAESDMRARALVEARVDADRLLLATQSALDVDGDLLGAEERVAMDA
                     AMQGLRSVLASEDTALIESQSKALAQATEAFAAMRMNRGIANALAGKNIATI"
     gene            4095..4433
                     /gene="fdx"
                     /locus_tag="PKX82_07175"
     CDS             4095..4433
                     /gene="fdx"
                     /locus_tag="PKX82_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HOF52072.1"
                     /translation="MPTIKILPHPEYCPQGAQVSAPAGTSICEALLDNGINIEHACDM
                     SCACTTCHVVVREGFASLNELDETEEDLLDRAWGLQPNSRLSCQALLAQADLVVEIPK
                     YSINHAKENH"
     gene            4436..>4941
                     /gene="dnaQ"
                     /locus_tag="PKX82_07180"
     CDS             4436..>4941
                     /gene="dnaQ"
                     /locus_tag="PKX82_07180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HOF52073.1"
                     /translation="MRQIFLDTETTGLYPDQGDRLIEIGCVELVNRKLTGNNLHFYVN
                     PGRDSHEEALKVHGITSEFLRDKPKFSEVVDAILSYVQDAEIIIHNAAFDLGFLNKEL
                     ELLGRPRFAEHVSGVVDTLAMAKEMFPGKRNSLDALCDRLDVDNSGRTLHGALLDAVE
                     GSSGVVYI"
BASE COUNT         1065 a         1371 c         1515 g          990 t
ORIGIN      
        1 gggcgcagcc cacttttacc agtccaaggg caaacacatc atcaccgtca agaccgagca
       61 caaggccgtg ctcgacacct gccgtgaact ggaacgccag ggctttgagg tgacctacct
      121 cgacgtgcag gaagatggtc ttctgaatct ggagtccctc aaggctgcca ttcgtcccga
      181 taccattctg gccagcgtca tgttcgtgaa caatgaaatt ggcgtgattc aggacattgc
      241 cgccattggt gcgatctgcc gtgaaaaagg cgtgatcttc catgtggatg cagcccaggc
      301 caccggccgc gtgccgtttg atctgtccca gctgccagtg gacctgatga gcctgaccgc
      361 ccacaagacc tacggcccca aaggcattgg cgcgctgttt gtgcgtcgca agccccgtat
      421 tcgcctggaa gcccagatgc atggcggcgg tcatgagcgc ggtatgcgta gcggtacgct
      481 gcctacccac cagatcgtgg gcatgggcga ggcgtaccgt attgccaaag aagaaatggc
      541 ggtagaaaac gtccgaatcc aggcgctgca cgaccgcatg ctggcgggtc tcaaagacat
      601 tgagcaggtg tttatcaacg gccatgtgga caagcgcgta ccccacaact tgaacatgag
      661 tttcaactat gtggaaggcg agtccctgat catgggtatc aagggcctgg ctgtgagcag
      721 tggctcggcc tgtacctcgg catctctgga gcccagctac gtgttgcgcg ctttgggccg
      781 cagcgatgag ctcgcccaca gcagcctgcg catgaccatc ggccgctgga cgaccgaagc
      841 ggacattgat tacgccgtgg acaccatcaa gaagaacgtt gcgaaactgc gcgaactcag
      901 ccccctgtgg gacatgtaca aggatggtat cgatatcagc accatccagt gggcagcaca
      961 ctgacaagtt gaagaggtaa aacaaaatgg catattccga aaaagtcgtt gatcactacg
     1021 aaaacccccg taatgtgggc agctttgaaa aaggggatga cacggttggc accggcatgg
     1081 tgggcgcacc tgcctgcggc gatgtcatga agttgcagat caaggtcaat ccccagacgg
     1141 gagtgattga agacgcacgt ttcaaaacct atggctgcgg ctcggctatt gcgtccagtt
     1201 cactggtgac tgaatgggtc aagggcaaga cgctggacga agcagccgcc ctgaagaaca
     1261 gccagatcgc cgaagagctg gctttgccac ccgtgaaaat tcactgctct atcctggcag
     1321 aggacgccat caaagccgct gtagacgact acaagaagaa acacctgaac tgatatggcc
     1381 gttactctga ccgaggccgc tgcgcggcac gtcacccggt atttgaccaa gcgtggcaag
     1441 ggcgtgggcg ttcgtctggg ggtcaagacc accggttgct cgggcttggc ctaccagctg
     1501 gaatacgtgg acgaactttc gcccgaagac gtggtgttcg agggccacgg tgtgaaggtg
     1561 ctggtagacc ccaagagtct ggcctacatc gatggtaccc aactcgactt tgtacgcgag
     1621 ggattgaacg aaggtttccg tttcaataac cccaacgagc gggacaagtg cggttgtggt
     1681 gaatcgttcc gcgtgtgaat cgccaggctg acgatttcga gttgtttggt ctggcccaac
     1741 agtttgcgca ggaccggatg gttttggacg cacgctggaa ggaacttcaa cgtgaagcgc
     1801 accccgacaa atttgcggcg caaggtgcgg cctcgcagcg tgtggccatg caatggtcgg
     1861 tgcgtatcaa cgaggcgtac cagcgcttga aggacccgct aagacgtgca gcctacctgt
     1921 gtgagctgca tggggcggcg atcaatgcgc acagcaatac tgccatgccg gcatccttcc
     1981 tgatgcagca gatggagtgg cgtgaagcgc tggaagacgc atcgtctgcg gccgcactgg
     2041 aagcgctgag tgatgaggtg caagctgccc ggctgcaact gctgcaacaa tgtgaggctt
     2101 tgctggatgg tcagcaggcg tatgcacagg cagttgagcc agtgcgtgcc ctgatgttta
     2161 tcgaaaaatt cacccaggac gtgaaccagc gctttgagca atttgaatag ttattaccat
     2221 ggcgctttta caaatttctg agccgggcca gtcgcccaac ccccacgaac gccgcattgc
     2281 agtcggcatt gacctgggaa ccacccattc gctggtggct gctgtgcgca acggcagtgc
     2341 ggagtgcctg ccagacaccc aaggtcgggt gctacttccc agtgttgtgc gctacattga
     2401 tgcacagcgc cgcgagattg gctttgacgc cctggcgcat cagacaacag accccggcaa
     2461 cacgatcgct tcggtcaagc gcctgatggg tcgcggagcc gcagatattg cccatgccga
     2521 aaagctgccc tatgcattgg ttgatgcacc ggggatggcg cagattcgca ccattgcggg
     2581 aaccaagagc ccagtggaag tcagcgccga aattctcgcc acgctgcgct tccgggcaga
     2641 agataccttt aacgatgata tctatggtgc ggtgatcacc gtgcccgcct attttgatga
     2701 tgcccagcgg caggcgacca aggacgctgc gcaattggct ggtctgaatg tgttgcggtt
     2761 gatcaatgag ccgaccgcag cagcgatcgc gtatggcctg gacaatgcgg ccgaaggcgt
     2821 ctacgccatc tatgacctgg gtggcggaac ttttgatatt tccattcttc gcctgtccgc
     2881 cggagtgttt gaggtgttgg ccacgggcgg cgactccgca ttgggcggtg atgactacga
     2941 tcgtgcgttg gcagaatggt tgctggccga gtcggacgtt ctggcccaaa cacccgaaga
     3001 ccaggcgttg gtgatggcgc aggcgcggtt gtgcaaggaa gccttgtctt ctgaagacat
     3061 cgtgactgct gaagtggatc tgtcgtcggg ttccattgac ctctcgctca accgggagca
     3121 gttcgaggcg gcgactgccg cgctcaccgc acgcaccctg caagctgttc gcaaagttct
     3181 gcgtgatgcc gcgttaggta aagacgatgt caaaggcgtg gtgatggtcg gtggctccac
     3241 gcggatgccg caggtgcagc gggctgtggc tgagttcttt ggccagccgc ccttgaccaa
     3301 tttgaatccc gatgaagtcg tcgcactggg ggccgcagtt caggcgaatc agctggctgg
     3361 aaacaatccc tctggtgatt tgctgctgct cgacgtgatc ccgctgtctc tgggcattga
     3421 gaccatgggg gggctggtgg agcgcattgt tccgcgtaac cagaccattc ctacagcgat
     3481 ggcgcaggac ttcactacct acaaggacgg gcagactgcg ctcgcactcc atgtggtgca
     3541 aggcgaacgc gatctggtgg cagactgccg cagtctggcg cgttttgagc tgcgtggcat
     3601 tcctccgatg gcagctggcg cagcgcgcat tcgggtcacg ttcaccgtgg acgcggacgg
     3661 tttgctgaac gtgtcggcaa aagagcaggt cagtggcgtg gaagcgcgca ttgacgtcaa
     3721 gccgtcctac ggtctgagcg atgaccagat tgccaaaatg ctttcggaaa gctttacgac
     3781 cgccgagtcc gacatgcgcg cgcgcgcgct ggtcgaagca cgtgtggatg cagaccgtct
     3841 gctgctggca acccagagtg ccctcgacgt ggatggtgac ctgttagggg cagaagaacg
     3901 cgtggcgatg gatgctgcga tgcaagggct gcgtagcgtg ctggcaagcg aggacacggc
     3961 gctgattgag agccaatcca aggcactggc acaggctacg gaagcctttg cggccatgcg
     4021 catgaaccgc ggcattgcca atgcactggc cggcaagaac atagcaacga tttgaatcgg
     4081 gagaccgact gaccatgcct actatcaaga tacttccgca ccccgaatat tgcccccaag
     4141 gcgcgcaggt gtcggcgccc gcaggtacct ccatttgcga agcactgctg gacaacggaa
     4201 tcaatatcga acatgcctgc gatatgagct gcgcctgcac cacctgccac gtggtggtgc
     4261 gtgaaggctt tgcttcgctc aacgaactcg atgagaccga ggaagacctg ctggaccgcg
     4321 cctggggtct gcagcccaat tcccgtctca gttgccaggc cttgttggca caggctgatc
     4381 tggtggtgga aatacccaaa tactccatca accacgccaa agaaaaccac tgaccatgcg
     4441 ccagatattt ctggataccg agacgaccgg cctctacccg gaccagggtg accgcctgat
     4501 agaaattggc tgtgtcgaac tggtgaaccg caaactcacc ggcaacaacc tgcatttcta
     4561 tgtgaacccc ggtcgtgaca gtcatgagga agcgctgaag gtgcacggaa tcacctcgga
     4621 gttcttgcgc gacaagccga aattttccga ggtggtggac gccatcttgt cctatgtgca
     4681 ggatgccgag atcatcatcc acaacgcggc atttgacctg gggttcctga acaaggaact
     4741 ggagctgctg ggccggccgc gcttcgctga gcatgtgtcg ggtgtggtcg atacgctggc
     4801 catggccaag gaaatgtttc ctggcaagcg caacagcctt gacgccctgt gtgatcgatt
     4861 ggatgtagac aactccggac gaaccctcca cggcgccctg ctggatgccg tggagggttc
     4921 gtccggagtt gtctacatcc a
//
LOCUS       DAOSJM010000170         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15300_length_4920_cov_4.357657, whole genome shotgun sequence.
ACCESSION   DAOSJM010000170 DAOSJM010000000
VERSION     DAOSJM010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15300_length_4920_cov_4.357657"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKX82_07185"
     CDS             <1..306
                     /locus_tag="PKX82_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOF52074.1"
                     /translation="LSPRAALEQYWARSIELAPPQIMSLSHLARHNRVDTVLAEARAQ
                     MPPLVCPEPHDANGERVLCYPGDTGHSIAQRALPGPTRLIYRNRRFEPTEGLEALFQ"
     gene            complement(316..1827)
                     /locus_tag="PKX82_07190"
     CDS             complement(316..1827)
                     /locus_tag="PKX82_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOF52075.1"
                     /translation="MDLFANLSLGFGVAFTAQNLMYAFVGCLLGTLIGVLPGIGPLAT
                     IAMLLPATYALPPVAALIMLAGIYYGAQYGGSTTAILVNLPGESSSVVTVIDGYQMAR
                     NGRAGPALAAAGLGSFFAGCVGTLILAAFAAPLTELAFKFGPAEYFSLMILGLIGAVV
                     LASGSLLKAVAMIVLGLLMGMVGTDVNSGVARFSFDIPELTDGIGFIVIAMGVFGYGE
                     IISNLSKHESERKVFTASVTGLMPTREDFKNMVPAVLRGTALGSLLGILPGGGAVMAA
                     FAAYTIEKKTKLRPGEVPFGKGNIRGVAAPESANNAGSQTSFIPLLTLGIPPNAVMAL
                     MVGAMTIHNIQPGPQVMTNNPELFWGLIASMWIGNLMLVILNLPLIGIWIKLLSVPYR
                     WLYPSIVLFCAIGVYSTNNNTFDIWMVGLFGVIGYLFIKLGAEPAPLLLGFILGPMME
                     EYLRRALLLSRGDWSVFVTRPISAGLLAAAVLLLVVVLMPSVKAKREEAFVED"
     gene            complement(1837..2325)
                     /locus_tag="PKX82_07195"
     CDS             complement(1837..2325)
                     /locus_tag="PKX82_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOF52076.1"
                     /translation="MNIKSQKDFFSGLMFMCVGVAFAWGASSYTIGNGARMGPGYFPL
                     VLGILLAVLGGAITFKALVVETFDGDHIGAIAWKPLFFIILSNLIFGAAIGGLPSIGL
                     PPLGLVVGIFLLTFVAANAGDEFKLKEVAILATLLAILSYAAFILLLNLQFPVWPAFI
                     KA"
     gene            complement(2392..4179)
                     /locus_tag="PKX82_07200"
     CDS             complement(2392..4179)
                     /locus_tag="PKX82_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOF52077.1"
                     /translation="MNKQHNHNAASRSVHAGPLDWRKLVEWLQKDGIVSTEEAQRTVA
                     RCSQAESVQHPLVRLASVSMRRLADNKPLDIEALTQWLAAHVGLDYLRIDPLKVDVGK
                     VADAMGAAYAERHRILPVVVSPSEMTVATSEPFVTDWVDELERQSRRKVRRVVASPQE
                     IHRYTAEFYALAKSVRAANKAGANSGASFEQLVELGKTNKQLDANDQGVVQVVDWLWQ
                     YAFDQRASDIHLEPRREQGVIRFRIDGVLHPVYQMPMGVLTAMTARIKLLGRMDVIER
                     RRPQDGRIKTRNPRGDEIEMRISTLPTAFGEKMVMRIFDPDNAVKDLDALGFSSHDAQ
                     RWEELVTRPHGIILVTGPTGSGKTTTLYSTLKRVATEEVNVSTVEDPIEMIEPAFNQT
                     QVQPQLDFGFPQGLRALMRQDPDIIMVGEIRDLETAEMAVQAALTGHLVFSTLHTNDA
                     PSAVTRLLELGIPSYLINATLLGVLAQRLVRTLCAQCRVRDEVSTRDALGEMVKPWQI
                     NGGYKPYKPVGCVDCRMTGFMGRMGLYELLVVTEAFKSRVSTDSSIEVLRRQAIADGM
                     RPLRLAGALRVAEGVTVMEEVLVSTPPLQ"
     gene            complement(4176..4919)
                     /locus_tag="PKX82_07205"
     CDS             complement(4176..4919)
                     /locus_tag="PKX82_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOF52078.1"
                     /translation="MHTLPEVNLSEEGPIRHLHLGSEWIQGSMLVDAPNVLVHEYIQR
                     MMAWMLFVDPATVAQRQALQLGLGAGSLTKFCHKELRMKTTAIELNPQVLHACRGWFK
                     LPADNTRMQVVLGDAAEEIRKPQWLGAVDALQVDLYDEEAAAPVLDSESFYADCRATL
                     TDDGCMTVNLFGRTSSFNGSVNKIAAAFGRDAIWSFKATREGNTVVLAQRTPSRPKRS
                     LLMERAEVIKAQWGLPADKWVRVFKPVNA"
BASE COUNT         1080 a         1574 c         1359 g          907 t
ORIGIN      
        1 ctatcccccc gcgccgcgct ggagcagtac tgggcacgca gtattgagct tgcgccacca
       61 caaatcatga gcctgtcgca tctggcgcgg cacaaccggg tcgataccgt tctggcagaa
      121 gcccgtgcac agatgccacc cctggtctgt cccgaaccgc atgacgctaa cggagaacgt
      181 gtactttgct accccggtga tacggggcac tccattgcac agcgggccct gcctggtcct
      241 acccgcctga tctaccgcaa ccgccgtttc gaacccaccg aaggcttaga ggcactattt
      301 cagtaaggcg atccatcaat cctcaacaaa agcttcttcc cgcttggcct ttaccgaagg
      361 catcagaaca accaccagca gcagtacggc agctgcaagc agccctgctg aaatcggccg
      421 ggtgacaaac acgctccagt caccccgcga cagcagcaag gcgcggcgca ggtactcttc
      481 catcatcggc ccgaggatga atcccagcag caacggagct ggctcggcac cgagcttgat
      541 gaacaggtaa ccaatcacgc caaacagccc aaccatccag atgtcaaatg tgttgttgtt
      601 ggttgaatag accccaatgg cacaaaacag cacaatggac gggtacagcc agcggtaagg
      661 tacggacagc agcttgatcc agataccaat gagcggcaga ttcagaatca ccagcatcag
      721 attgccgatc cacatcgatg cgatcaggcc ccaaaagagt tcggggttgt tcgtcatgac
      781 ctgggggcca ggctggatgt tgtggatggt catcgccccg accatcagcg ccatcaccgc
      841 gttgggggga atccccaatg tcagcaacgg tatgaacgat gtctgtgaac cagcgttgtt
      901 ggccgactcc ggtgctgcaa cgccacgaat gttgcccttg ccaaacggta cctctccagg
      961 gcgaagcttg gttttcttct cgatggtata ggcagcaaag gccgccatga ccgcaccacc
     1021 gccaggcaaa atccccagga gggagcccag cgccgtaccg cgcagtacgg ctggcaccat
     1081 gttcttgaag tcttcccggg ttggcatgag gccagttacc gaggcggtaa acactttgcg
     1141 ctcgctctcg tgtttggaaa gattgctgat gatctcgcca tagccaaaca ctcccatggc
     1201 aatgacgatg aagccgatac catccgtcag ctccggaatg tcaaagctga accgggcaac
     1261 acccgagttc acatccgtgc ccaccatgcc catcaacagg cccaacacaa tcatggcaac
     1321 cgccttcagc aaagaccctg aagcaaggac cacagcacca atcaagccca ggatcatgag
     1381 cgaaaaatat tccgcaggac caaacttgaa agccagctct gtcagcggcg ctgcaaaggc
     1441 cgccagaatc agtgtcccca cacatccggc aaagaaggaa cccaatcccg ccgcagccag
     1501 tgccgggcca gcccgaccgt tgcgggccat ctggtaacca tcaataaccg tcactaccga
     1561 agaagattca ccgggcaggt tcaccagaat ggctgtcgtc gatccaccgt actgcgctcc
     1621 atagtaaata ccggccagca tgatgagcgc agccacagga ggcaaggcgt aggtggcagg
     1681 caacaacatg gcaatggtgg ccagggggcc aatgccgggc agcacaccaa tgagcgttcc
     1741 tagcaggcag ccgacaaacg catacatcag gttctgggcg gtgaaggcaa cgccaaagcc
     1801 cagggaaaga ttggcaaaca ggtccatggt gtttcctcag gctttgatga aggcaggcca
     1861 caccgggaac tgcaagttga gcagcagaat gaacgctgca tagctcaaga tcgccagaag
     1921 tgtggcgaga atagccactt ccttgagttt gaattcatct ccggcattgg cagccacaaa
     1981 cgtcaacaag aagatgccaa ccaccagccc caggggcggc aagccaatcg acggcagccc
     2041 accaatggcg gcaccaaaaa tcagattcga caggatgatg aaaaagagtg gtttccaggc
     2101 aatcgctccg atgtggtcac cgtcgaaggt ttccaccacc agcgccttga acgtgatggc
     2161 gccccccagc actgcgagca gtatgcccag caccagcggg aagtagcccg gtcccatgcg
     2221 cgcgccattg ccaatggtgt aggaactggc accccaggca aacgccacac caacgcacat
     2281 gaacatcagt ccggagaaaa agtctttctg actttttata ttcattacct gtctcctcgt
     2341 atcaaacggt gtggcgtatt ctgcactgaa atgccaggct gtcagaacgg tctactgcag
     2401 tggcggagtg gataccagca cctcttccat gaccgtcaca ccttcggcga cccgcaaggc
     2461 cccggccagg cgcaagggcc gcatgccatc agcgatggcc tggcgccgca ggacttcaat
     2521 actggagtca gtactgaccc gcgacttgaa tgcctcggtc accacgagca gttcatacag
     2581 acccatacgg cccatgaatc ccgtcatgcg gcagtccacg caaccgaccg gcttgtaggg
     2641 cttgtatcct ccgttgatct gccagggctt gaccatctca cccagagcat cacgggtaga
     2701 cacctcatcg cgcaccctgc actgggcgca cagcgtgcgc accagccgct gggccagcac
     2761 cccgagcaag gtggcattga tcagatagga aggaatgccc agttccagca ggcgcgtcac
     2821 cgctgaaggc gcgtcattgg tgtgcagcgt agaaaacacc agatgccccg tcagcgcggc
     2881 ctgcacggcc atttctgccg tttcgaggtc gcggatttca cccaccatga tgatgtcagg
     2941 gtcctggcgc atcagggcgc gcaggccctg cgggaatccg aaatccagct gcggctgcac
     3001 ctgcgtctgg ttgaacgcag gctcaatcat ttcgatcgga tcttccaccg tactcacgtt
     3061 gacctcttcg gtggccaccc gcttcagcgt ggaatacagc gtcgtggtct tacccgaacc
     3121 ggtgggcccc gtcaccagaa tgatgccgtg cgggcgcgtg acaagctctt cccagcgctg
     3181 cgcgtcgtgg ctggagaagc ccaacgcatc cagatccttc accgcgttat cgggatcaaa
     3241 aatccgcatc accatcttct cgccaaacgc cgtcggcaag gtagagattc gcatttcgat
     3301 ctcgtcaccg cgcggattgc gggtcttgat gcggccgtcc tgggggcgtc gccgctcgat
     3361 cacgtccatg cgtcccagca gcttgatgcg cgccgtcatg gcagtgagca ctcccatggg
     3421 catctgatac accgggtgca gtacgccgtc gatgcggaac cgaatgacac cctgctcacg
     3481 ccgcggctcc agatggatat cgctggcccg ctggtcaaac gcgtactgcc atagccagtc
     3541 caccacctgg accacgccct ggtcgttggc gtcaagctgc ttgttggtct tgcccagctc
     3601 caccagctgc tcaaagctgg cgccagagtt ggcaccggct ttgttggccg cgcgcaccga
     3661 cttggccagg gcatagaact cggcggtgta gcggtgtatt tcctggggac tggcgactac
     3721 gcggcgcacc ttgcgacgcg actggcgctc caactcatcg acccagtccg taacaaaagg
     3781 ttcggatgta gccactgtca tttcgctggg cgacaccacc accggcaaga tccggtggcg
     3841 ttctgcatag gccgcgccca tggcatcggc caccttgccc acatccacct tgagcggatc
     3901 aatacgcagg taatccagcc cgacatgggc tgccagccat tgtgtcaatg cctcgatatc
     3961 cagcggcttg ttgtctgcca ggcggcgcat ggacacgctg gccagccgaa ccagcgggtg
     4021 ctgcacactc tcggcctggg agcaacgcgc aaccgtgcgc tgggcctcct cggtcgacac
     4081 gattccatct ttttgcagcc actccaccag cttgcgccag tccagcggac cggcatggac
     4141 gcttctggac gccgcgttgt ggttgtgctg cttgttcatg cgttcaccgg tttgaaaacc
     4201 cgcacccatt tgtcggcggg caggccccat tgcgccttga tgacctccgc gcgctccatc
     4261 aggagggacc gcttgggacg ggatggtgtg cgctgggcca gcaccacagt gttgccttcg
     4321 cgcgtggcct tgaagctcca aatggcatcg cgcccaaacg ccgcggcgat cttgttcaca
     4381 ctgccattga aactggaggt ccggccgaac agattcaccg tcatgcagcc atcatccgtc
     4441 agtgtggcgc gacagtccgc ataaaacgac tcgctgtcga ggaccggtgc cgcggcttcc
     4501 tcgtcgtaca ggtcgacctg caaggcatcg acggcaccca gccactgcgg cttgcgaatt
     4561 tcctccgccg catcccccag caccacctgc atgcgggtgt tgtctgctgg cagcttgaac
     4621 cagccccggc aggcgtgcag cacctgcggg ttgagctcga tggctgtggt cttcatcctc
     4681 aactccttgt ggcagaactt ggtcagcgac cctgccccca gaccgagctg cagagcttgt
     4741 cgttgcgcca cagtagccgg atcgacaaac agcatccagg ccatcatgcg ctggatgtat
     4801 tcatgcacca gcacattcgg cgcatccacc agcatggagc cctgtatcca ttcgctgccc
     4861 aggtgcagat ggcggatcgg accttcttcg gagagattga cttcagggag tgtgtgtatt
//
LOCUS       DAOSJM010000171         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15373_length_4904_cov_4.134461, whole genome shotgun sequence.
ACCESSION   DAOSJM010000171 DAOSJM010000000
VERSION     DAOSJM010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15373_length_4904_cov_4.134461"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2458
                     /locus_tag="PKX82_07210"
     CDS             <1..2458
                     /locus_tag="PKX82_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOF52079.1"
                     /translation="TVVEPLRADWANAQAASIVLAQEAAELSGKAHDAKMAEARAEVH
                     KFHHQLCTTRVLDPACGSGNFLYVTLEHLKRLEGEVLNQLEAFGFHTDADLQDSLGLE
                     GETVTLEQLRGIELNERAAALAELVLWIGFLQWHIRTRGNKAVAEPVVHNYGNIECRD
                     AVLAWDDLEPAYDAHGVLLTRWDGKTFKTHPVTGAQVPDEAAQVPQWKYLNARKAAWP
                     QVDFVVGNPPFIGKLKLREALGDGYIEALRSVSPDVPDSADFVMYWWAHAAKLVATHQ
                     IRRMGLITTNSLTMVYNRRLVEWAQAAGTHLHFAIADHPWVDSANGAAVRIAMTVLGP
                     GEGEGTLFAVTSEETGEHGEISVSLTSKIGLIHADLSVGANVAKAEQLTANQGMSHMG
                     VILVGSGFIIQPGDPLLQLEPGPIRQYMNGRDLTQTSRGVFVIDLAGLTQEESRSKYP
                     NCYQRLYDTVRLERSVSRDPYLRDNWWLFRRTNMQLRSGLAGLKRYIGTTETAKHRTF
                     SFIDGAVLPDQKIRVVTSDDAFYLGVLSSTTHVAWALASGGTLEDRPVYNNTRCFETF
                     PFPGQDTGLTPEVRDRISNLAEQIDTHRKTVMANSGAADLTLTGLYNVLEALREGCEL
                     TAKEKAIHTQGLVGVLKDLHDELDTAVLQAYGWADLHAVASDTPELLERLVALNARRA
                     AEEQAGTVRWLRPEFQSPATPSHPETLQNQELPAQEPRGLQADLALNAPIEEAKPAKL
                     RKGASATASVAVQAWPATLPEQVRAVAAVLASANAALPLDALEARFKSRGAWKKSLPR
                     ILETLEALGRARREGDGWRS"
     gene            2494..2847
                     /locus_tag="PKX82_07215"
     CDS             2494..2847
                     /locus_tag="PKX82_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HOF52080.1"
                     /translation="MKATFVELPPFARLRADYMDDEAYRALQNELMQNPEAGDVIEGT
                     GGLRKVRQADPRRGKGKRGGLRVIYYWWLGGAQFWLFTVYDKDQADDLTADQRRSIKR
                     LLDTERQSRQVKDAT"
     gene            2844..3158
                     /locus_tag="PKX82_07220"
     CDS             2844..3158
                     /locus_tag="PKX82_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOF52081.1"
                     /translation="MSKRDIFSEIVEGFDALAAERKGKLTLRHVQVESKPVPVLEPQE
                     VLAVREKLHLSRQVFARYLRTNPRTLENWEQGRAKPNAQAAVLIRMVAKFPDMVQRLE
                     TV"
     gene            3192..3482
                     /locus_tag="PKX82_07225"
     CDS             3192..3482
                     /locus_tag="PKX82_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOF52082.1"
                     /translation="MNLQDPAVQEFCEAHRIRKLALFGSQVKGTAGADSDVDLLVEFE
                     PDSVPGLFGMAALEEELSHLLGGRKVDLRTARELSRHFREEVVRTAHVQYAR"
     gene            3489..3773
                     /locus_tag="PKX82_07230"
     CDS             3489..3773
                     /locus_tag="PKX82_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOF52083.1"
                     /translation="MLVAIWYATASVITFLVYAIDKSAARQRTWRTPERTLHLLALAG
                     GWPGALLAQQLLRHKTAKLPFRITFWITVLLNVAGSLWICSPAGRATLNL"
     gene            complement(3872..>4904)
                     /locus_tag="PKX82_07235"
     CDS             complement(3872..>4904)
                     /locus_tag="PKX82_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF52084.1"
                     /translation="AKAGTGGPQLLRTMVGAEEIAEVVSRATGIPVSKLMQGERDKLL
                     QMETALHKRVVGQDEAIVAVSDAIRRSRAGLSDPNRPLGSFLFLGPTGVGKTELCKAL
                     AGFLFDSEEHLIRVDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVLLL
                     DEVEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQMIQSMVGQDYE
                     DVKEAVTGELKNHFRPEFLNRIDETVVFHSLDASHIANIAKIQLATLTTRLAKMDLAL
                     EVSDAAVAELAKVGFDPVFGARPLKRAIQQRIENPLSKLLLEGKFAPKDTIHVGVDTI
                     QAPGQFSFS"
BASE COUNT         1047 a         1521 c         1457 g          879 t
ORIGIN      
        1 caccgtagtg gagccgctgc gcgccgactg ggccaacgca caggccgcct ccatcgtgct
       61 ggcgcaggag gcggcggaac tcagtggcaa ggcccacgac gccaagatgg ccgaagcccg
      121 cgccgaagtg cacaagttcc accaccagct ctgcaccacc cgcgtgctgg acccggcctg
      181 cggcagcggc aacttcctgt atgtcacgct ggagcacctc aagcgcctgg agggcgaagt
      241 gctcaaccag ctggaggcct ttggcttcca caccgatgcc gacctccagg acagcctggg
      301 cctggaaggc gaaacggtca cgctggagca actgcgcggc atcgagctca acgagcgcgc
      361 cgccgccctg gccgagctgg tgctgtggat cggcttcttg cagtggcaca tccgcacccg
      421 cggcaacaag gcggtggccg aacccgtggt gcacaactac ggcaacatcg aatgccgcga
      481 cgccgtgctg gcctgggacg acctggaacc cgcctacgac gcccatggcg tactgctcac
      541 ccgctgggac ggcaagacgt tcaagacgca cccggtcact ggcgcacagg tgcccgatga
      601 agcggcacag gtgccgcagt ggaaatacct gaatgcccgc aaggcagctt ggccacaggt
      661 ggactttgtg gtgggcaacc cgccgttcat tggaaaactc aagcttcgtg aagcgttggg
      721 agacggctat atcgaagcgt tgcgcagcgt ttcgcctgac gttccagaca gcgcagattt
      781 cgttatgtat tggtgggcac acgcggcgaa gctagtcgca acacaccaga tacgtcgcat
      841 gggactgatc accaccaaca gtctgaccat ggtctacaac cgcaggctgg tggagtgggc
      901 gcaggcggcc ggtacacatt tgcactttgc aattgcagat cacccttggg tggatagcgc
      961 caacggcgcc gcagtacgta ttgcgatgac cgtattggga ccaggtgaag gcgaaggaac
     1021 tctgttcgcc gtcaccagcg aggaaactgg tgaacatgga gaaatttcgg tttcgttgac
     1081 aagcaagatt ggattgatac acgctgattt gagtgtcggc gccaatgttg caaaagcaga
     1141 gcaactgaca gcaaatcaag gtatgtcaca tatgggtgtg atcctggttg gctcgggatt
     1201 catcatccag cccggtgacc ctctgcttca actagagcca ggtcctatca ggcaatacat
     1261 gaatggtcgg gatttgacac agacaagccg cggcgtattt gtcattgacc ttgcaggact
     1321 tacacaggaa gagtcacgct caaaatatcc aaattgctat caacggctat atgacaccgt
     1381 gcgccttgaa aggtcagtga gtcgcgatcc gtacctacgg gacaactggt ggcttttcag
     1441 acgaacgaac atgcagcttc gcagcggatt ggctggactc aagagataca tcggcaccac
     1501 agaaacagcc aagcaccgga ccttctcctt tatcgacgga gcagttcttc cagaccagaa
     1561 gatacgcgta gtgacttctg acgacgcttt ttatctaggt gttctttcga gcacaaccca
     1621 tgtggcttgg gcattggcta gtggcgggac ccttgaggat cgccctgtct acaacaatac
     1681 ccggtgtttc gagactttcc cattccccgg ccaagacact ggcctcacac ctgaagtgcg
     1741 cgaccgaatc tccaacctgg cagagcaaat cgacacacac cgcaagacgg taatggcgaa
     1801 ttcgggagca gcagacctca ccctgaccgg cctgtacaac gtcctcgaag ccctgcgcga
     1861 gggctgcgaa ctcacggcca aagagaaggc catccacacc cagggcctgg tgggtgtgct
     1921 caaggacctg cacgacgagc tggacaccgc cgtactgcag gcctacggct gggccgacct
     1981 gcacgctgtc gcatccgaca cgcccgagct gctggagcgt ctggtggcgc tcaacgcccg
     2041 ccgcgccgcc gaagaacagg cgggcaccgt gcgctggctg cgcccggagt ttcagagccc
     2101 ggcaacacca tcgcatccag aaacgctaca aaatcaggag ctacctgcgc aggaaccacg
     2161 ggggctacag gccgatttgg cactcaacgc tcccatagaa gaggccaaac ccgccaaact
     2221 gcgcaagggc gcatctgcta ctgcatcagt cgcagtgcaa gcctggcccg ccaccctgcc
     2281 cgagcaggtg cgcgccgtgg ccgccgtgct ggccagcgcc aacgctgcct tgccgctgga
     2341 cgcactggaa gcccgcttca aaagccgcgg tgcctggaaa aaaagcctgc cccgcattct
     2401 ggaaacgctg gaagccctgg gccgcgcgcg gcgcgagggg gatgggtgga gaagttaagc
     2461 caaatatgta cgtaacttgc gtataattcc gccatgaaag ccacgttcgt tgaacttcct
     2521 ccatttgccc gcctgcgagc ggactatatg gacgatgaag cctaccgcgc cctgcagaac
     2581 gagttgatgc agaacccgga ggcgggtgat gtcatcgaag gcacgggtgg cctgcgcaaa
     2641 gtcaggcaag cagacccgcg cagaggcaaa ggcaagcgcg gcgggctgcg cgtgatctac
     2701 tactggtggc taggcggtgc acagttttgg ctgtttacgg tatatgacaa ggaccaggcc
     2761 gacgatctga ctgcagatca acgtagaagt atcaagcggc tgctagacac agagcgacag
     2821 agccgccaag taaaggatgc aacatgagca aacgcgacat attttcagaa atcgtcgaag
     2881 ggttcgatgc cctggcggca gaacgcaaag gcaagctgac gctacgccat gtgcaggtgg
     2941 agtccaaacc tgttcccgtg ctggagccgc aggaagtgct ggcagtgcgt gaaaagctgc
     3001 acctgtcgcg ccaggtattt gcccgctatt tgcgaaccaa cccccgcact ctggaaaact
     3061 gggagcaagg ccgagccaaa cccaatgccc aggcagctgt gctcattcgc atggtggcga
     3121 agtttcccga catggtgcaa cgcctggaga ccgtttagtc cttcccaccc agaggcccta
     3181 tgcaccgcga aatgaatctt caggaccccg ccgttcaaga attctgcgaa gcccaccgga
     3241 ttcgtaaact ggcattgttt ggatcacaag tcaagggcac agcgggtgcc gacagcgacg
     3301 tggatttgct ggttgagttt gagccggaca gcgtgcctgg gctctttggt atggctgcgc
     3361 tggaagaaga actctcccac ctattaggcg gacgcaaggt ggacctacgc accgcccgtg
     3421 aactctcaag gcactttcgg gaagaagttg tccgcacagc ccatgtgcaa tatgcgcgct
     3481 gaagaccaat gttggtagct atctggtacg ccacggcgag cgtcattacc ttcctcgttt
     3541 acgccatcga caaatcagcg gcgagacaac gcacctggcg caccccggag cgcaccctgc
     3601 acctgctggc attggctggc ggttggcccg gcgcgctgct ggcacagcag ctattgcggc
     3661 acaaaaccgc caaactgccg ttccgcatca ccttctggat cacggtgctc ctgaacgtag
     3721 cgggctcgct gtggatctgc tcacccgcag gtcgcgccac cctcaaccta taaaaaagcc
     3781 agccaggatt tgctaccaaa tccatagctg gctgcgcata ttgcacgggg gctagcggcc
     3841 caaagggcca ctaaaccacc cgtatcaggc atcaggagaa gctgaactgc cctggcgcct
     3901 ggatcgtgtc gacccccacg tggatcgtgt ccttgggcgc gaacttgcct tcgagcagca
     3961 atttcgagag cgggttctcg atgcgctgct ggatggcgcg cttgagcggt cgggcaccaa
     4021 acaccgggtc gaagcccact ttggccagct cggccacggc agcgtcgctc acctccagcg
     4081 ccaggtccat cttggccagg cgcgtggtca gcgtggccag ctggatcttg gcaatgttgg
     4141 caatgtgcga tgcgtccagc gaatggaaca ccacggtctc atcgatccgg ttcaggaact
     4201 cggggcggaa gtggttcttc agctcaccgg tcaccgcttc cttcacgtcc tcgtagtcct
     4261 gccccaccat gctctggatc atctgcgagc cgatgttgct ggtcatcacg atcacggtgt
     4321 tcttgaaatc cacggtgcgg ccctggccat cggtcaggcg accatcgtcc agcacctgca
     4381 gcagcacgtt gaacacatcg gggtgggcct tttccacctc gtcgagcagc agcacgctgt
     4441 agggcttgcg gcgcacggcc tcggtcaggt agccgccctc ttcgtagccc acatagccgg
     4501 gaggcgcgcc aatcaggcgg gccaccgaat gcttctccat gaactcgctc atgtccacgc
     4561 ggatcaggtg ctcttcgctg tcaaacagga agcccgccag cgccttgcac agctcggtct
     4621 tgcccacgcc cgtggggccc aggaacagga agctgcccag cggccggttc ggatcagaca
     4681 agcccgcgcg gctgcggcgg atggcgtcgg acacggccac gatggcctcg tcctggccga
     4741 ccacacgctt gtgcagcgcg gtctccatct gcagcagctt gtcgcgctcg ccctgcatca
     4801 gcttgcttac cgggatgccg gtggcacgtg acaccacctc ggcaatctct tcggcgccca
     4861 ccatggtgcg cagcagctgc gggccgccgg tgccggcctt ggca
//
LOCUS       DAOSJM010000172         4903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15377_length_4903_cov_4.999587, whole genome shotgun sequence.
ACCESSION   DAOSJM010000172 DAOSJM010000000
VERSION     DAOSJM010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15377_length_4903_cov_4.999587"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..450)
                     /locus_tag="PKX82_07240"
     CDS             complement(46..450)
                     /locus_tag="PKX82_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOF52085.1"
                     /translation="MTITYTIGDLAREFDLTTRAIRFYEDLGLLQPERSGPAGRSRIY
                     SGRDRTRLKLTLRAKRLGLSLTEAKDIIDMYDSPRDTAAQLERFLQVLSVHQAQLDAQ
                     MKDLQANMDELKVHQREARALLKKAQGQAHAA"
     gene            555..1637
                     /locus_tag="PKX82_07245"
     CDS             555..1637
                     /locus_tag="PKX82_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52086.1"
                     /translation="MNDLEQQLHYPMGDALPELGQCITVAPGVKWIRMALPFALNHIN
                     LWLLRDEIHGQQGWSIVDCCIHKEEAKTQWESIFANELEGLPVLRVIVTHMHPDHIGL
                     AHWLCDRWNAPLWISAADYQIARFGCMGPTAFGGDRAADFFAKHGLNSPDIVAQIRAR
                     TNYFPSLVPSVPDQYCRMLDGGKLTIGPNVWRCISGYGHAPEHMALYCETLNVLIGGD
                     MMLPRISTNVSVYEQEPEANSLALFLDSIDRFKALPEDTLVLPSHGKPFTGMHTRIGQ
                     LHDHHRARLAEVMQACSSRACSAADIISVLFTRALDLHQTTFAIGESIAHLHALWFEG
                     KLARHLDADGVYRFIATAAVPGQQRH"
     gene            complement(1641..1931)
                     /locus_tag="PKX82_07250"
     CDS             complement(1641..1931)
                     /locus_tag="PKX82_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HOF52087.1"
                     /translation="MAAYLVVDTLLDNPELYEEYKRLARPLAERFGGEYLARGGEMTL
                     KETDLWSPTRLVLVRFPDAQSANSFYDSPEYQDVLKISKQSARRTVVVLEGV"
     gene            complement(1968..2900)
                     /locus_tag="PKX82_07255"
     CDS             complement(1968..2900)
                     /locus_tag="PKX82_07255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOF52088.1"
                     /translation="MHPLLRYTLDLFDAAPLPVPAPRAPVRRRRVPGGHISKPFEAAA
                     PVDTAQPAIENIAPSAFVHPKATREVRLGDAVVAYAFARGKRRTIGFVVGPDGLAVRA
                     PRWTPLSEVDAALQEKASWILRKLHETRERHMRQQGARIDWADGVTFPLLGNAVQVVL
                     DPGHHFAAQGSHLDVPQDEDSASLLGVALPHTASAEQIRDAVQAWLMRYAKALFIERL
                     DHFAPQLQVQWRHLSLSNAGTRWGSARADGSIRLNWRLVHFSPSVIDYVVVHELSHLR
                     VMDHSPRFWDTVRTVVPDYASLRGQLKDEAVPRW"
     gene            complement(2918..3655)
                     /locus_tag="PKX82_07260"
     CDS             complement(2918..3655)
                     /locus_tag="PKX82_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOF52089.1"
                     /translation="MSFLRSVVHMLWMLVTVIPWAIIMVVASTRVRGKPLWWMAVRWL
                     RWATDGARVILGVRPHVIGMENLPTGDTAPAILLVKHQSTFETFFMPTLMPHPLAYVF
                     KKELLYVPFFGWAMGRLDMIHIDRSQGSRSFRKVVAQGKALLAQGIWIIMFPEGTRVD
                     RGQKGSYKSGGTRLAIETGAPVIPVAVTSAKCWPRKAFIKTPGVVEISIGKPIPSAGR
                     QPDELMREVEAWIEAEMRRLDPEAYTD"
     gene            complement(3676..4224)
                     /gene="gmhB"
                     /locus_tag="PKX82_07265"
     CDS             complement(3676..4224)
                     /gene="gmhB"
                     /locus_tag="PKX82_07265"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HOF52090.1"
                     /translation="MKLCILDRDGTINEDSAEYVKSPGEWQPLPGALEAIAKLNHAGW
                     HVVVVTNQSGLGRGLFDVAALNAMHAKMHTMLAAVGGKVDAIFYCPHAPDEACHCRKP
                     EPGLFEQIGERYGLDLKGLPAVGDSQRDLVAAYAAGCEPHLVLTGKSEGLRDRPLPEG
                     YPPNTVVHEDLSAFADFLVARG"
     gene            complement(4233..>4903)
                     /locus_tag="PKX82_07270"
     CDS             complement(4233..>4903)
                     /locus_tag="PKX82_07270"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOF52091.1"
                     /translation="GIGNLPSGDKDPFALRRHALGVIRMLVEKDLALDVTALVQSAVP
                     AFGDRVTDASAALVDFMFERLAGSLREQGYSAQEVESVLALRPQRLGDVAKRLAAVRA
                     FAALPESPALAAANKRISNILKKTDDVDAHVSPVLLQEAAEKALYAAMQTVLPQAQSQ
                     WEAGDYTASLQSLAALRAPVDAFFEDVMVNAEALDLRLNRLGLLKSLHLAMNRVADLS
                     KLAA"
BASE COUNT          907 a         1572 c         1531 g          893 t
ORIGIN      
        1 agggtccatt gcaacccgac gcgcgcgctg tgcggaccca ctgcgtcagg ctgcatgggc
       61 ctgtccctgg gctttcttga gcagggcgcg cgcctcgcgc tggtgcacct tgagctcatc
      121 catattggct tgtaaatctt tcatttgggc gtcaagttgc gcctggtgca cgctgagcac
      181 ctgcaaaaac cgctccagct gcgcagcggt gtcgcgcggg ctgtcgtaca tgtcgatgat
      241 gtccttggcc tcggtcaggc tcaggcccag ccgcttggcg cgcagggtga gcttgagacg
      301 ggtgcggtca cggccgctgt agatgcggct gcgtccggca gggccgctgc gctcgggctg
      361 cagcaggccc agatcctcgt aaaagcggat ggcccgggtc gtcagatcga actcgcgggc
      421 gaggtcgcca atggtgtagg tgatggtcat aatcaattcg caacatgcgc gacagcacgc
      481 cgtgcgttcg ctgtctacaa tgcccaatcc agttgacgtt tacgtaaacg tcaattatga
      541 agcaaaaagg caccatgaac gatctggaac agcaactgca ttacccgatg ggtgacgccc
      601 tgcccgagct gggccagtgc atcacagtcg cgcctggcgt caagtggata cgcatggcgc
      661 tgccgttcgc gctcaaccac atcaacctct ggctgctgcg agacgaaatc cacggccagc
      721 agggctggag catcgtggac tgctgcatcc acaaggaaga agccaagaca cagtgggaat
      781 ccatttttgc caacgagctc gaaggcctgc cagtgctgcg cgtcatcgta acgcacatgc
      841 accctgacca catcggcctg gcgcactggc tgtgcgaccg ctggaatgcg cccctgtgga
      901 tcagtgcggc tgactaccag attgcccgct ttggctgcat gggccccacc gcctttgggg
      961 gcgaccgggc tgctgatttc tttgcaaaac acggactgaa ctccccggac atcgtggcgc
     1021 agatccgggc gcgtaccaat tactttccct cgctggtgcc atcggtgccg gaccagtact
     1081 gccgcatgct cgacggcggc aagctcacga tcggccccaa cgtctggcgc tgcatcagcg
     1141 gctacggcca tgcgcccgag cacatggctc tgtattgcga aacgctcaac gtcctgatcg
     1201 gcggcgacat gatgctgccg cgcatttcca ccaatgtcag cgtgtacgag caggagcccg
     1261 aagccaattc actggcactg ttcctggatt ccatcgaccg gttcaaggcc ttgccggaag
     1321 acacgctggt gctgccgtcc cacggcaaac cgtttaccgg catgcacacc cgcattggcc
     1381 agctgcacga ccaccaccgt gcccggctgg ccgaggtgat gcaggcctgt agcagcaggg
     1441 cctgcagcgc ggcggacatc atttccgtgc tgttcacgcg tgcactggac ctgcaccaga
     1501 ccacgttcgc catcggcgaa tcgattgccc acctgcatgc gctgtggttt gaaggaaaac
     1561 tcgcccgcca cctcgatgcc gatggcgtct accgcttcat cgccacagct gcagtgccag
     1621 ggcagcaacg gcactaggtt ctacacccct tcgagtacca ccaccgtccg gcgagcagac
     1681 tgcttgctga tcttgagcac gtcctggtat tcgggagagt cgtaaaaact attggcgctc
     1741 tgggcatcgg gaaaccgcac cagcaccagg cgtgtgggcg accagaggtc ggtttccttg
     1801 agggtcatct cgcccccgcg ggccaggtat tcgccaccga agcgctctgc cagcggtcgg
     1861 gccagccgct tgtattcttc atacagttcg gggttgtcca gcaaggtgtc aacgaccagg
     1921 taggctgcca tcgggttctc cagtggttgg gtgaaacccg gagcctatca ccagcgggga
     1981 acggcttcgt ccttgagctg gccacgcagg ctggcgtaat cgggcaccac ggtgcgcacg
     2041 gtatcccaga agcgggggct gtggtccatc acccgcaggt ggctgagttc gtgcaccacg
     2101 acgtaatcga tgaccgacgg actgaagtgg acgagccgcc agttcaggcg gatgctgccg
     2161 tctgcccgtg cactgcccca gcgggttcct gcattgctca ggctcagatg ccgccactgc
     2221 acctgcagct ggggcgcaaa gtggtccagc cgctcgatga acagcgcttt cgcgtaacgc
     2281 atcaaccagg cctgtaccgc atcgcggatc tgttctgctg acgcagtgtg gggcagcgcc
     2341 acccccaaga gggacgcgct gtcttcgtcc tgcggtacgt cgagatggga gccctgggct
     2401 gcgaaatggt ggccagggtc cagcaccacc tggacggcgt tgcccagcaa cggaaaggtg
     2461 acgccatcgg cccagtcaat acgggcgccc tgctggcgca tgtggcgttc acgggtctcg
     2521 tggagttttc gcaagatcca cgacgccttt tcctgcaggg ctgcgtccac ttcggacaaa
     2581 ggcgtccagc gcggcgcacg caccgccaga ccatcgggtc ccaccacaaa accgatggtg
     2641 cgccgcttgc cccgcgcaaa ggcataggcc accaccgcat cgccaagacg cacttcacgg
     2701 gtcgctttgg gatggacgaa tgccgatggc gctatgtttt cgatagctgg ttgcgcagta
     2761 tctacggggg ctgcagcctc aaaaggcttg gaaatatgac cgccgggcac gcgacgcctg
     2821 cgcaccggag ccctgggtgc tggcaccggc agtggtgccg catcgaacaa atccagcgtg
     2881 tagcgaagca gcgggtgcat ggtggctgcg ccggccctca gtctgtgtac gcctcggggt
     2941 ccaggcggcg catttcggcc tcgatccagg cttccacttc gcgcatcagt tcatcgggct
     3001 ggcggccggc gctagggatg ggttttccaa tggaaatttc caccacgcca ggggttttga
     3061 tgaaggcctt gcgtggccag catttggcgg aggtcactgc gaccggaatg accggtgcac
     3121 cggtctcgat ggccagccgc gtaccgcccg acttgtagct ccccttctgg ccccggtcga
     3181 cccgcgtacc ttcgggaaac atgatgatcc agatgccctg ggccagcagt gccttgccct
     3241 gggccaccac cttccggaac gaccttgagc cctggctgcg gtcaatatgg atcatgtcca
     3301 gccgccccat ggcccatccg aaaaacggca cgtagagcag ttctttcttg aacacatagg
     3361 ccagcggatg cggcatcagt gtgggcatga aaaaggtctc gaaggtggac tggtgcttga
     3421 ccagcaggat ggccggagcg gtgtcgccag tcggcaggtt ttccatgcca atcacatggg
     3481 gacggacgcc caggatcaca cgggccccgt ccgtggccca gcgcaaccag cggaccgcca
     3541 tccaccacag tggtttgcct cgcacacggg tggaggcgac caccatgatg atggcccagg
     3601 gaatgaccgt caccagcatc cacaacatgt ggacgaccga acgcaaaaac gacaatgtgc
     3661 gtgagggctg cgcgctcatc cgcgggccac cagaaagtcg gcaaatgccg acaggtcctc
     3721 atgcaccacg gtgttgggcg ggtagccttc cggcaaaggc cggtcacgca gcccttcgga
     3781 tttgcctgtc agcaccagat gcggctcgca ccctgcagcg tacgcggcca ccagatcccg
     3841 ctgggaatca cccaccgcag gcagaccctt gaggtccagt ccgtagcgtt cgccgatctg
     3901 ctcgaacagg cctggctccg gcttgcggca gtggcaggct tcatcgggtg catgggggca
     3961 ataaaaaatg gcatcgacct tgccgccgac ggcggccagc atggtgtgca tcttggcgtg
     4021 catcgcattg agtgcggcca catcgaacaa accgcgcccc aggcctgact ggttggtgac
     4081 caccaccaca tgccaaccgg catggttgag tttggcaatg gcttccaggg cgccgggcaa
     4141 gggctgccac tcgccgggag acttgacgta ctccgcactg tcttcgttga tggtgccgtc
     4201 gcggtcgaga atgcaaagtt tcatgggatg gattacgcgg ccagtttgga caaatcggcc
     4261 acgcggttca tcgcgaggtg caggctcttg agtaggccca gtcggttcag gcgcaggtcc
     4321 agcgcctccg cgttgaccat cacatcctca aagaacgcat cgaccggcgc gcgcaaggca
     4381 gccagcgact gcagcgacgc ggtgtagtcg cccgcctccc actggctttg ggcctgtggc
     4441 agcacggtct gcatggcagc gtacagcgcc ttctcggcgg cttcctgcag cagaacggga
     4501 ctgacatggg cgtccacatc gtcggtcttc ttgaggatgt tgctgatgcg cttgttggcg
     4561 gctgccagcg cagggctctc gggcagggct gcgaaggcgc gcaccgcggc caggcgcttg
     4621 gccacatcgc ccagccgctg cggacgcagg gccagcacgg actccacttc ctgcgcactg
     4681 tagccctgct cacgcaggga tccggccaga cgctcgaaca tgaagtccac cagcgcggcc
     4741 gaagcgtcgg ttaccctgtc accaaaggca gggaccgcgc tttgcaccag cgcggtcaca
     4801 tccagcgcga ggtctttctc caccagcatg cggatcacgc ccagcgcgtg gcggcgcagc
     4861 gcgaacgggt ccttgtcgcc gctgggcagg ttgccgatgc cga
//
LOCUS       DAOSJM010000173         4885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15443_length_4885_cov_4.817391, whole genome shotgun sequence.
ACCESSION   DAOSJM010000173 DAOSJM010000000
VERSION     DAOSJM010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15443_length_4885_cov_4.817391"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..2514)
                     /gene="pheT"
                     /locus_tag="PKX82_07275"
     CDS             complement(79..2514)
                     /gene="pheT"
                     /locus_tag="PKX82_07275"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOF52092.1"
                     /translation="MQFPESWLRAFCNPPISTAVLAETLTMVGLEVEELKPVAPPFTQ
                     IVVGEIKTAEQHPNADRLRVCTVDVGQTELLNIVCGAPNARVGIRVPCALVGAELPPG
                     EDGKPFVIKVGKLRGVESQGMLCSAKELQLADDHGGLLELAADAPVGQNIREHLNLDD
                     TLFTLKLTPNLAHCLSVYGVAREVSALTGSPLLTPVIAPVAVTNQDTVPVKISAPDLC
                     GRFSGRVVRNVNTKAKTPQWMVDRLARCGQRSVTALVDISNYVMFEFGRPSHIFDLDK
                     IHGTLDVRWGKAGEELKLLNGNTVTVDDKVGVIADDVQVESLAGIMGGDATAVSDDTK
                     NVYVEAAFWWPKSVAGRSRRYNFSTDAGHRFERGVDPELTVEHIERITQLILEICGTP
                     ETTCGPVDDLQPNVPKPAPVSLRVARAAKVIGMPVTQTQCVEVLQRLCLPLTQTDGVI
                     TVTPPAYRFDIQIEEDLIEEVVRIVGYNNLPNTPPLAPISAKVRTESQRSPFAVRRAL
                     AALGYTETINYSFVEDRWEHELAGNPDPIKLLNPIASQMNVMRTSLLGSLLQVLKFNL
                     ARKAPRVRVFEIGRVFLRDVSVPESDSTVQGFDQPMRIAALSSGNANAQQWGHKEQGV
                     DFFDAKGDVEALLAPQMAEFKPGNHPAMHPGRCAQVLVGGKVIGHVGELHPKWRQGYE
                     LPQAPMLFELELDPILARKLPEFVPVAKYQPVQRDIAVVVADSVTYADLMAAVWAAST
                     VGLLRDAQLFDVYRPKVAKEVPVEAGATDRSLAIRLTFNSTEATLTDDQIETAVKAVV
                     DQLQSTVGARQRV"
     gene            complement(2523..3596)
                     /gene="pheS"
                     /locus_tag="PKX82_07280"
     CDS             complement(2523..3596)
                     /gene="pheS"
                     /locus_tag="PKX82_07280"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOF52093.1"
                     /translation="MNDLQPIVDAAQAAFAQAATPAELENAKAQFLGKSGRMTELMKG
                     MAALSVEEKKTRGAAINLAKQAIEAALNAARQAMADAELAAQLQAEALDVTLPGRQRG
                     QGGLHPVSLAQERIEAIFGSMGFDVAQGPEIEADWFNFTALNTPEDHPARSMHDTFYV
                     EGGSATAPNLLRTHTSPMQIRYAVQHVKKYRALAGDSGDGLFGGAMPEIRVIAPGRTY
                     RVDSDATHSPMFHQVEGLWVGENISFKDLKSVYLNFITTFFETDALQLRFRPSYFPFT
                     EPSAEIDIMFESGPLKGRWLEVSGSGQVHPQVIRNMGLDPERYIGFAFGSGIDRLAML
                     RYGVNDLRLFFDGDVRFLSQFQN"
     gene            complement(3713..4072)
                     /gene="rplT"
                     /locus_tag="PKX82_07285"
     CDS             complement(3713..4072)
                     /gene="rplT"
                     /locus_tag="PKX82_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOF52094.1"
                     /translation="MPRVKRGVTARARHKKVLALAKGFRGRRGNVFRIAKEAVMKAGQ
                     YAYRDRRTKKRVFRQLWIARINAAARQCGMTYSQFANGLKKANIEIDRKVLSDIAIHD
                     MAAFAGIVEQVKAKLAA"
     gene            complement(4098..4301)
                     /gene="rpmI"
                     /locus_tag="PKX82_07290"
     CDS             complement(4098..4301)
                     /gene="rpmI"
                     /locus_tag="PKX82_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOF52095.1"
                     /translation="MPKMKTKSAAKKRFRVRPGGTVKRGQAFKRHILTKKTTKNKRHL
                     RGSAAVHETNMGHMAQMLPGRGI"
     gene            complement(4469..>4885)
                     /gene="infC"
                     /locus_tag="PKX82_07295"
     CDS             complement(4469..>4885)
                     /gene="infC"
                     /locus_tag="PKX82_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOF52096.1"
                     /translation="EIAATANPPVCRLMDYGKFKYQEQKKAAEAKAKQTVIEIKEIKF
                     RPGTDDGDYNIKMRNIKRFLAEGDKCKITLRFRGREITHQEIGLAMLQRMRDELGDTI
                     IVEQFPKLEGRQMIMMIAPGRKKPGGAPKPAAEAAA"
BASE COUNT          993 a         1451 c         1405 g         1036 t
ORIGIN      
        1 aaaagcagat cggccaactg cgctttggtc agtgccggag tttccaggct ttcaacagaa
       61 aaatccatag attcctcctc agacacgctg gcgcgcgccg acggttgact gcaattggtc
      121 caccactgcc ttgacagcgg tttcaatctg atcgtccgtc agcgtagcct cggtgctatt
      181 gaaagtcaga cgtatagcca gactgcggtc ggtggcaccg gcctcaacag gcacctcctt
      241 ggcgaccttg gggcggtaca catcaaagag ctgggcatcg cgcaacaggc caacagtgga
      301 agcggcccac actgcggcca tcagatcggc gtacgtcacg gagtcggcta ccaccacggc
      361 aatgtcacgc tgcacaggtt ggtacttggc cacgggtacg aattccggca gtttgcgcgc
      421 aagtatggga tcgagctcaa gttcgaacaa catcggagct tgcgggagct cgtagccctg
      481 gcgccatttc gggtgcagtt cacccacatg accaatcact ttgccaccca ctagcacctg
      541 ggcgcaacgg ccagggtgca ttgcagggtg attgccgggc ttgaattcgg ccatctgcgg
      601 ggccagcaag gcctcaacat cgcctttggc gtcgaagaaa tcaacgccct gctccttgtg
      661 accccactgc tgcgcattcg cgttgccgct ggacaatgca gcaatgcgca tgggctggtc
      721 aaagccctgc acggtactgt cggactcggg caccgacacg tcgcgcagga acacgcggcc
      781 gatctcgaat acgcgaacac gcggcgcctt gcgagccagg ttgaacttca gaacctgcag
      841 cagggaaccc agcagcgacg tgcgcatcac gttcatctgg cttgcaatcg ggttcagcaa
      901 cttgattgga tcggggttgc cggccagttc atgctcccag cggtcttcga cgaagctgta
      961 gttgatcgtc tccgtataac ccaacgcggc cagcgcacgg cgcacggcaa acggactacg
     1021 ctgcgactcg gtccgcacct tggccgaaat cggcgcaagc ggcggcgtgt tcggcaagtt
     1081 gttgtaaccg acgatgcgca ccacctcttc aatcagatct tcttcgatct ggatgtcgaa
     1141 acgatacgcg ggtggggtca ccgtgattac gccatcggtc tgcgtcaacg gcaggcacag
     1201 acgctgcaac acttccacgc actgcgtctg tgtgaccggc atgccgatca ccttggctgc
     1261 gcgcgccacg cgcaggctca ccggagcggg tttgggcaca ttgggctgca gatcgtcaac
     1321 aggcccgcag gtggtctcag gcgttccgca aatctccagg atcaactggg taatgcgctc
     1381 gatgtgctcc accgtcagtt cgggatccac accacgctcg aagcggtgcc ccgcatcggt
     1441 agagaagttg tagcgacgcg aacggcccgc aaccgacttg ggccaccaga acgcggcctc
     1501 gacgtagacg tttttcgtgt cgtctgacac ggcagtggca tcgccgccca tgattccggc
     1561 cagcgactcg acctggacat cgtcagcaat cacaccgacc ttgtcgtcca ccgtgacggt
     1621 gttgccattg agcagcttga gctcttcgcc cgctttgccc catcgcacgt ccagcgtgcc
     1681 gtggatcttg tccagatcga agatgtggga tgggcgaccg aactcgaaca tcacgtagtt
     1741 ggagatgtcc accagagcgg tgacgctgcg ctgaccgcag cgcgccaggc ggtcgaccat
     1801 ccactgcggc gtcttcgcct tggtgttgac gttgcgaacc acgcgaccgg agaagcggcc
     1861 gcacagatcg ggcgcgctga tcttgaccgg cactgtgtcc tggttcgtca cagcaaccgg
     1921 tgcaatgaca ggcgtcagca aaggcgaacc ggtcagtgca gacacctcac gcgcgacacc
     1981 gtacacgctc aggcaatggg ccagattggg cgtgagtttg agtgtgaaca gagtgtcgtc
     2041 caggttcagg tgttcacgga tgttctggcc cacaggtgca tccgcagcca actccagcaa
     2101 accaccgtga tcgtccgcca actgcaattc tttggcagag cacagcatgc cctggctttc
     2161 cacaccacgc agcttgccga ccttgatcac aaagggcttg ccgtcttcac cgggaggcag
     2221 ctcggccccg accagcgcgc aaggcacgcg gatacccaca cgcgcattgg gcgcaccgca
     2281 gacaatattc agcagttcgg tctgaccaac atccacagtg cagacacgca gacgatcagc
     2341 attggggtgt tgctcggcgg tcttgatttc acccacgacg atctgggtga acggaggcgc
     2401 aacgggtttg agctcctcca cttccagacc gaccatggtg agcgtctcgg caagcacagc
     2461 ggtcgagatg ggaggattgc agaaggcgcg gagccaggat tcagggaatt gcatagttct
     2521 aatcagttct ggaattgcga caggaaacgg acgtcgccat caaagaacag gcgcaggtcg
     2581 ttcacgccgt agcgcaacat ggccagacgg tcgatgccgg agccgaaagc aaagccgatg
     2641 tagcgctccg ggtccagacc catgttacgg atcacttgcg ggtgcacctg gcccgagccg
     2701 gagacttcca gccagcgccc cttgagcggg ccagattcga acatgatgtc gatctcggcg
     2761 cttggctcgg tgaacggaaa gtaactgggg cggaaacgca gttgcagtgc gtccgtctcg
     2821 aaaaatgtcg tgatgaagtt caggtagacc gacttcaaat ccttgaagct gatgttctcg
     2881 ccaacccaca acccctccac ttggtggaac atcggagaat gggtcgcatc actgtccacc
     2941 cggtaggtgc ggcccggcgc gatcacgcga atctcgggca tggcaccgcc aaacagcccg
     3001 tcgccggagt cacctgccag tgcacggtat ttcttgacgt gctgcaccgc atagcggatc
     3061 tgcatcgggc tggtgtgggt gcgcagtagg ttgggggctg tggcggaacc accttccacg
     3121 tagaaggtgt cgtgcatcga acgtgcagga tggtcttcgg gggtgttgag tgccgtgaaa
     3181 ttgaaccagt cggcctcaat ttccgggcct tgagcgacat cgaagcccat ggatccgaat
     3241 atggcttcaa tacgctcttg cgcaagtgac accggatgca agcccccctg cccgcgctga
     3301 cggccgggca gcgtcacgtc cagcgcttca gcttgcagct gggcggccag ttctgcatcg
     3361 gccatggctt gacgggctgc attcagtgcc gcctcaatcg cctgtttggc cagattgatg
     3421 gccgcaccac gggttttctt ttcttccaca ctcagggcgg ccataccctt catcaactcg
     3481 gtcatgcgcc cggacttgcc gaggaactgg gctttggcgt tttccagttc tgctggggta
     3541 gcggcttgtg caaaagcagc ttgcgcggcg tcgacaatgg gctggaggtc gttcatgggt
     3601 gcggtgtaaa actgtatcgg agagaattga aaaagggcta gtgctctttc aagccctagc
     3661 ccttgtttct ggtgcttggg tagctataaa aactatagcc acctatcgcg aatcaagcag
     3721 ccagcttggc cttgacttgt tccacgatgc cagcaaaagc agccatgtca tggatagcaa
     3781 tatcggacag aaccttgcgg tcgatctcga tgttggcttt cttcagacca ttggcaaact
     3841 ggctgtatgt cataccgcac tgacgtgcag cagcgttgat acgggcgatc cacaactgac
     3901 ggaacacacg tttcttggta cgacggtcac ggtaggcata ttgcccagcc ttcattaccg
     3961 cttctttggc gatacggaag acattaccgc ggcgaccacg gaaacctttt gcaagggcca
     4021 gaactttttt atggcgggcg cgagccgtta caccacgttt gacgcgaggc atgtgattac
     4081 tccttgttcg tcgttaatta aataccacgg ccggggagca tctgtgccat gtgacccata
     4141 ttggtctcat gaacagctgc agaaccgcgc agatggcgtt tatttttggt ggtcttcttg
     4201 gtcagaatgt gacgtttgaa ggcttgaccg cgcttgacgg tgccacctgg acggacgcgg
     4261 aagcgtttct tcgccgcgct cttggtcttc attttgggca tttaaatgct ccttttcatt
     4321 tgtgctcgtg aggcgtctgg aaactgcttc cagccttgtt ggccccgagc cacttgtgtg
     4381 gggagatttg catctctcca cttgcgtagc ggatttctca acccgccacc tttgctatca
     4441 gccggtgacc agaccgaccg aaagaaactt acgccgcagc ttccgctgcg ggcttgggtg
     4501 caccacccgg ctttttacgc cccggtgcaa tcatcatgat catctggcgc ccttccagct
     4561 tcggaaactg ctccacgata atcgtgtcac ccaactcgtc gcgcatacgc tgcaacatcg
     4621 ccagaccaat ttcctggtgg gtgatttcgc gaccccgaaa acgcagagtg atcttgcact
     4681 tgtcgccttc ggccaggaag cgcttgatat tgcgcatctt gatgttgtag tcgccgtcat
     4741 cggtacccgg acggaacttg atctctttga tctcaataac cgtctgcttg gctttcgctt
     4801 ccgccgcctt cttctgttcc tggtacttga acttgccgta atccatcaaa cgacagaccg
     4861 gcggattcgc cgtagcagca atttc
//
LOCUS       DAOSJM010000174         4869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15499_length_4869_cov_4.950353, whole genome shotgun sequence.
ACCESSION   DAOSJM010000174 DAOSJM010000000
VERSION     DAOSJM010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4869
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15499_length_4869_cov_4.950353"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKX82_07300"
     CDS             complement(<1..298)
                     /locus_tag="PKX82_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOF52097.1"
                     /translation="MTHNLYAQRRAALAAHIGPKGVAILPTAPEQQRNRDSDFLFRHD
                     SYFYYLSGFTEPKAWLVVTGDGKTSLFCQPKDLEREIWDGFRLGPDAAPGALGVD"
     gene            complement(337..1110)
                     /locus_tag="PKX82_07305"
     CDS             complement(337..1110)
                     /locus_tag="PKX82_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOF52098.1"
                     /translation="MLLAAGRGERMRPLTDTCPKPLLQVQDKPLMQWPLEALARGGFD
                     QVVINTAWLGEQIEQQFGSIFGLQPPSNMRNQLSNQEQSTASMHIQYSHEGRDFGGAL
                     ETAGGIARALPQLGEVFWVLAGDVYAPDFVFSQDAVTRFVQSDMLAHLWLVPNPEHNL
                     AGDFGLSAATGNLPGLALNLPKDTPEPRYTYSTIGLYRRGLFEAPWCDIAPGNPTGTK
                     AALAPLLRRAMDHGKVSAELYTGRWTDVGTPQRLAQLNA"
     gene            1487..1816
                     /locus_tag="PKX82_07310"
     CDS             1487..1816
                     /locus_tag="PKX82_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOF52099.1"
                     /translation="MGKKLYVGNLSYSVDDHSLNQNFSDFGTVSSAKVMMDRDSGRSK
                     GFGFVEMSSDAEAQAAISGMNGKNVDGRDMVVNEARPMEPRTGGGGGYGGGRSGGGGY
                     GGGGNRY"
     gene            complement(1874..2614)
                     /locus_tag="PKX82_07315"
     CDS             complement(1874..2614)
                     /locus_tag="PKX82_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52100.1"
                     /translation="MSHPPQPDPTHEILTSYYWSGDVLRSRSVGGVVLSGTVDIPAPP
                     ARLVADWERETALRLGLEPGDVEALSLPRARMRWPDYKRCVQTASDWMRTRDLHDVLA
                     ASDVALMACRGARYHHDAALYGGAAFCNLFLSADKGLDVHFPSIGLRIPLVRGTVLVF
                     DTGQPHAVIARHSAGFDVADFALAKDCTQLFLTWELPIEHADVAQALGVAFDTDPASA
                     LQLEEEQVWCHGARASVCPDSGRWCLAT"
     gene            2793..3341
                     /gene="phaP"
                     /locus_tag="PKX82_07320"
     CDS             2793..3341
                     /gene="phaP"
                     /locus_tag="PKX82_07320"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HOF52101.1"
                     /translation="MTTVNVEQIVAANKTAVADAQEIGTIALAGFEKLVALNMAAAKS
                     ALFDTSADFMAAFSTQNPTDALAAQASLVKPLAEKSLAYSRSVYAIASETSAELNKIA
                     EGKMAESQKAMSEAVATMAKNAPAGSETVVAMFTSAVNAGQNAIETAKSSAKKAMEMA
                     EKQTTSAVETALSSVKTPSRKK"
     gene            complement(3405..4526)
                     /locus_tag="PKX82_07325"
     CDS             complement(3405..4526)
                     /locus_tag="PKX82_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOF52102.1"
                     /translation="MSLLFTPFSLPSPRGGLTLPNRIVVAPMCQYSAVDGLAADWHLM
                     HWGNLLNSGAGLVTIEATAVLPEGRISPGCLGLWDDATEAAMTDTLQRARRLAPATAV
                     CIQLSHAGRKGSSAAPWHGGQLLSAADGGWETFGPSALPHLPTEAPPTVMSLEYMAQV
                     RDAFVSAAQRAQRMGIDAIELHAAHGYLLHQFLSPLANQRTDAYGGSFENRIRFVLDV
                     FTAVRAAYQGVLGLRLSATDWVDGGWDLEQSTALAHRLKAAGCDFIHVSSGGVSPQQK
                     ITLGVGYQVPFARQIRAACGMATMAVGLITEPQQAEDVLQAGDADLVALARAFLYQPR
                     WGWQAAAALGGTVAAKEPYWRCLPREAQAAFGKVSIGMR"
     gene            4624..>4869
                     /locus_tag="PKX82_07330"
     CDS             4624..>4869
                     /locus_tag="PKX82_07330"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452058.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOF52103.1"
                     /translation="MANTQQMASAIRALAMDAVQQANSGHPGAPMGMADMAVALWGEH
                     LSHNPGNPNWLNRDRFVLSNGHGSMLIYALLHLTGYKL"
BASE COUNT          966 a         1529 c         1477 g          897 t
ORIGIN      
        1 cgtccacgcc cagcgcgcca ggtgcggcat ccgggccgag ccggaagcca tcccagatct
       61 cgcgctccag gtccttgggc tggcaaaaca aactggtctt gccgtcacca gtgaccacca
      121 gccaggcctt gggctcagta aagccgctca ggtagtaaaa gtagctgtca tgccggaaca
      181 gaaagtcgct gtcgcggttg cgctgctgct cgggcgcggt gggcaaaatg gcaacgccct
      241 tggggccgat atgggcggcc agggcggcgc ggcgttgtgc gtacaggttg tgtgtcatag
      301 cgtggtgtcc atcgtggacg gtgtgcaaaa gtagtttcag gcgttgagtt gggccaggcg
      361 ttgcggtgtg ccgacatcgg tccagcggcc cgtgtagagc tcggcgctca ctttaccgtg
      421 gtccatcgcc cggcgtaaga gcggggccaa tgccgccttg gtgccggtgg ggttgcccgg
      481 cgcgatgtcg caccacggtg cttcaaacag gccacgccgg tacaggccga tggtgctgta
      541 ggtgtagcgg ggctcgggtg tgtccttggg caggttcagg gctagcccag gcagattgcc
      601 agtggcagcg ctcaatccaa agtcgcccgc caagttgtgc tccgggttgg gcaccagcca
      661 caggtgggcc agcatgtcgc tctgcacgaa gcgtgtcacc gcgtcctgcg agaacacaaa
      721 gtcgggcgca tacacgtcac ctgccagcac ccagaacacc tcgcccagct ggggcagcgc
      781 acgcgcaatg ccgccggcgg tttccagcgc gccgccaaaa tcacggcctt cgtgggagta
      841 ctggatgtgc atgcttgctg tggattgctc ctgatttgat agctggttgc gcatgttcga
      901 cgggggctgg aggccaaaaa tgcttccaaa ctgctgctcg atctgctcgc ccagccacgc
      961 cgtgttgatc accacctggt caaaaccgcc acgggccaac gcttccaggg gccattgcat
     1021 cagcggtttg tcctgcacct gcagcagggg tttggggcag gtgtcggtca gcgggcgcat
     1081 gcgctcgccg cggccggcgg ccagcagcat ggcggtggca gaaggcgggg tggaggtggt
     1141 gttcacggtg aagcaattcg aaaaagcagc caatcgagca tagcccatca tcaaacaacc
     1201 caccgtggtt gctcaatcga gcgtaaaata cacgcaccgc tgcaatttgc ttacctttgc
     1261 agtggttgta gtctgacagg ctctgcggca tcacccacgc tgcacccaca ccttcctggc
     1321 cgatagtctc ggcccttccg gtcgtcaaac tcccctgtgc ggcgagcttt acttccggtc
     1381 ctatttctca ctccgtttcc tcagatggtt ttttaaccaa gaccgtcatt ccccctgcgt
     1441 cgcgcggttc attgaaactg cggtcgcttt tttgaaagat ccctccatgg gcaagaaact
     1501 ttacgtcggc aacctctcct actcggtaga tgaccattcc ctcaatcaaa acttctctga
     1561 cttcggcacc gtttcgtctg ccaaggtcat gatggaccgc gacagcggcc gctccaaggg
     1621 cttcggcttt gtggaaatgt ccagcgacgc ggaagcgcag gcggctatca gcggcatgaa
     1681 cggcaaaaac gttgacggcc gcgacatggt ggtcaatgaa gcccgtccca tggagccacg
     1741 taccggtggc ggtggtggtt acggcggtgg ccgctccggc ggcggtggct atggtggtgg
     1801 cggcaaccgc tactaagacc tgcactgcag cgcaagagac cggccttttt ggccggtttt
     1861 ttttcgtccg gcgtcaggta gccaggcacc accggcccga gtccgggcat acgctggccc
     1921 gtgcaccatg gcaccagacc tgttcttcct ccagttgcaa tgcgctagcc gggtcggtgt
     1981 caaaagcgac tcccagcgcc tgcgcgacat cggcatgctc gatgggcagt tcccaggtca
     2041 ggaacagctg ggtgcaatcc ttggcaaggg caaagtcggc cacatcaaag ccggcgctgt
     2101 gacgcgcgat gacggcgtgg ggctggcctg tatcgaagac cagaaccgta ccccggacca
     2161 gggggatgcg aaggccgata gacgggaaat gcacgtccag tcccttgtcc gcgctcaaaa
     2221 acagattgca gaaggccgca ccgccgtaga gtgctgcatc gtggtggtac cgcgcgccac
     2281 ggcaggccat gagggccacg tcgctggcgg ccagcacatc gtgcaggtcg cgcgtgcgca
     2341 tccagtcaga cgccgtctgc acacaacgct tgtagtccgg ccagcgcatg cgtgcgcgcg
     2401 gcaaggacag ggcctcgacg tctccgggct ccagacccag gcgcaacgca gtttcccttt
     2461 cccaatccgc gaccagcctt gccggcggcg cagggatgtc gaccgtgcca gacagcacga
     2521 caccccccac actgcgactg cgcagaacat cgccactcca gtaataggag gtgaggattt
     2581 cgtgcgtggg gtccggttga ggggggtgac tcatcctgac cagtgtattc aagccagact
     2641 ttaggccgtc acatctagat tgctgcaatg cagcaaaaat tgcttgcatt cggcgcgaat
     2701 cgccctaaga tacgactcat gctgcactgc aacattcatt cgccaccggc ggatttgtag
     2761 cgcagaagtt cttaacctct ttggagaaac acatgactac agtgaatgtt gaacaaatcg
     2821 ttgccgccaa caagaccgcc gttgctgacg cgcaagagat cggtacgatc gccctggctg
     2881 gctttgagaa gctggtcgct ctgaacatgg ctgccgccaa gtcggccctg tttgatacca
     2941 gcgccgattt catggctgct ttcagcaccc agaatcctac ggatgcactg gccgcccagg
     3001 cttcgctggt gaagcccctg gctgaaaagt ccctggccta cagccgttcg gtgtacgcta
     3061 tcgcctctga aaccagcgcc gagctgaaca agattgccga aggcaagatg gcagaaagcc
     3121 agaaagccat gtccgaagcc gttgcaacca tggccaagaa tgctcctgcc ggcagcgaga
     3181 ccgtggtggc catgttcacc tctgctgtga atgcgggtca aaacgccatc gaaactgcca
     3241 agtcgtctgc caagaaggcg atggaaatgg ctgagaagca aaccacctcg gctgtggaaa
     3301 ctgccctgag ctcggtcaag actccttccc gcaagaagta atcttcggcg agggtcttca
     3361 aaaggcacgt cctgttatgg ggcgtgcctt ttgtcatttt gaattcagcg catcccgatc
     3421 gacaccttgc caaaagctgc ctgtgcctcg cgcggcaggc agcgccagta gggttctttt
     3481 gcagccacgg tgccacccag tgcggcggcc gcctgccagc cccaacgcgg ctggtacaga
     3541 aaagcccgcg ccagcgccac cagatccgca tcgcctgctt gcagcacgtc ttcggcctgc
     3601 tgcggctcgg tgatcaagcc caccgccatc gtcgccatac cgcaggcagc gcggatctgc
     3661 cgggcaaacg gcacctggta acccaccccc aatgtgatct tctgttgtgg cgacacgcca
     3721 ccgctggaca catggataaa gtcgcagccc gcagctttca gccggtgggc cagcgcggtg
     3781 ctctgttcca gatcccagcc gccgtccacc cagtcggtag cggacaaacg tagccccagc
     3841 accccctggt aggctgcgcg cacggcagta aacacgtcca atacaaagcg gatgcggttc
     3901 tcgaaactgc cgccataggc atcggtgcgc tggttcgcca gcggcgagag aaactggtgc
     3961 agcagatagc cgtgggcggc gtgcaattca atagcgtcga tacccatgcg ctgtgcccgt
     4021 tgcgcggctg aaacaaatgc atcgcgcacc tgtgccatgt attccagcga cattaccgtg
     4081 ggcggtgcct cggtgggcag gtgtggcagc gctgaggggc caaacgtttc ccagccgcca
     4141 tcggccgcgg acaacagttg tccaccgtgc caaggggcag cactggagcc tttgcgcccg
     4201 gcgtgcgaga gctggatgca gaccgcggtt gcaggagcca gccggcgtgc gcgttgcagg
     4261 gtgtcggtca tcgccgcctc ggtggcgtcg tcccacaaac ccaggcatcc cggcgagata
     4321 cggccttcgg gcaacacggc ggtcgcctcg atcgtgacca gaccggcgcc actgttgagc
     4381 aggttgcccc agtgcatcaa atgccagtcg gccgcgaggc catcgaccgc cgagtactgg
     4441 cacatgggcg caaccacaat gcggttgggc agagtcagcc cgccgcgcgg tgacgggagg
     4501 gaaaaaggag tgaaaagcag gctcatgaac acaagcatag ttcaaccgga attcccgccc
     4561 ggtaaaatac cgggtttacc ccctccagcc cctaactcct tttaccccgg agccgccccc
     4621 caaatggcca atacccaaca gatggcaagt gcaatccgcg cacttgcaat ggacgcagtg
     4681 caacaagcca actccggtca ccccggcgcc ccgatgggca tggcagatat ggcggtggcc
     4741 ttgtggggcg agcacctcag ccacaaccct ggtaacccca actggctcaa ccgcgaccgc
     4801 tttgtgctgt ccaacggcca cggctccatg ctgatttacg cgctgttgca cctgaccggc
     4861 tacaagctg
//
LOCUS       DAOSJM010000175         4862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15521_length_4862_cov_4.812773, whole genome shotgun sequence.
ACCESSION   DAOSJM010000175 DAOSJM010000000
VERSION     DAOSJM010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15521_length_4862_cov_4.812773"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PKX82_07335"
     CDS             complement(<1..328)
                     /locus_tag="PKX82_07335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOF52104.1"
                     /translation="MLSPVRQRQAHPPNFSLPEFRSALGMFATGVTIVTARTAQGSLV
                     GLTANSFNSVSMSPPLVLWSLARTAGSMAAFSAGSHYAINILSASQQDLARQFAAQGV
                     DRFANVE"
     gene            531..1256
                     /locus_tag="PKX82_07340"
     CDS             531..1256
                     /locus_tag="PKX82_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HOF52105.1"
                     /translation="MCESAGLRDMQARSVQGRAIYGRDIQPDDARLRVLVIGGIHGDE
                     MSSSALVFHWISLASVPLSDTQLPVHWRFIPAINPDGLFARPARRTNASGVDLNRNFP
                     TPNWARDARVYWENRTRKDPRRWPGNKPLSEPESRFLHEQMNTFKPNLIVSVHAPYGV
                     LDFDGPSVPPSRLGRLYLDQVGIFPGSLGNYGGIHKRVPVVTIELPHALRTPQDAEIR
                     QMWIDLMRWTDEKLGPRLVAASP"
     gene            1320..2780
                     /locus_tag="PKX82_07345"
     CDS             1320..2780
                     /locus_tag="PKX82_07345"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF52106.1"
                     /translation="MKIIDVAIVGAGFGGLCAAIRLRESGVDNVAILERSGEVGGTWR
                     DNVYPGCACDVPSHMYSFSFEPNTRWTRPYPQQPEIQDYILRLTDTYRLRPLIRFHSD
                     VRSMRWLAEQNCWELAVEGQPPLLARHVVLATGPLSKPAIPDLPGLGSFAGETFHSGH
                     WRHDVNLKGKRIAVVGTGASAIQFVPEIAPDAAHVDVFQRTAAWVMPRWDKPYGAARL
                     WLYRHVPGLQRLSRWRVYWFNEMVGMGFMGSRRVQNMLRKLSGHHLQRQVADPALRQA
                     LTPDFNPGCKRLLISNTWFPTLQRPNVQLVTQGVARIAPEGVVGADGALYPCDVIVWG
                     TGFKATEFVAPMQVYGESANGEPLELGKLWGAQPAATRLGITVAGFPNLFLLVGPNTG
                     LGHNSIIFMIECQVHYIVKALQSLRASGKNALRLRPDVQRDDYAVMQQKMKSTVWSSG
                     CKSWYQNAQGEIDTLWPGYTWEYWLQTRRFKASDYL"
     gene            complement(2797..3540)
                     /locus_tag="PKX82_07350"
     CDS             complement(2797..3540)
                     /locus_tag="PKX82_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOF52107.1"
                     /translation="MSEPIITFDKVNKWYGNNFHVLRDIDLTVTKGERIVVCGPSGSG
                     KSTLIRCINRLEEHQQGHLTVDGVELTDDVKAIDDVRKKVGMVFQQFNLFPHLTVLEN
                     LTLSPMWVGKLPKKEAEEKAMEQLRRVRIAEQASKYPLQLSGGQQQRVAIARALCLTP
                     KIMLFDEPTSALDPEMIKEVLDVMIEMAGQGITMICVTHEMGFAKAVADRVIFMDQGQ
                     IVEQNNPHDFFNHPQSERSRDFLSKILGH"
     gene            complement(3550..4650)
                     /locus_tag="PKX82_07355"
     CDS             complement(3550..4650)
                     /locus_tag="PKX82_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOF52108.1"
                     /translation="MTSKTFQPIPPRPAPVRTEGALAWCKINLFSGWQTTLGTLLVGG
                     VLLALLPRLVQWALIDAVWVADYQACHDASGACWGVVREKFRFILFGRYPFDEHWRAL
                     ICTVMLLALIMASCTRAFWKPWLGALWAVVLVVYFTLMRGGVLGLERVETDLWSGLPL
                     TILLASLSMVMAFPIALFIALGRSSAMPAIRSLCTVYVELIRGVPLISVLFMASFMFP
                     LLMPQGFNIDVLIRVLAGITLFAAAYMAEVIRGGLQAIPKGQIEAAATLGLSYWQTQR
                     KIVLPQALAMVVPGIMNNFISTFKDTSLVAIVSLYELTGAMGMAMNSDANWRPFRIEG
                     YLFIALIYFVFCYSMSRYSQWIEAQVNKSKLR"
     gene            complement(4660..>4862)
                     /locus_tag="PKX82_07360"
     CDS             complement(4660..>4862)
                     /locus_tag="PKX82_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOF52109.1"
                     /translation="LNLTKNSSLAVAIGYPDVVSIANTAINQTGRAVECIAIIMLVYL
                     STSLSTSLLMNWYNARAAIKER"
BASE COUNT          911 a         1486 c         1547 g          918 t
ORIGIN      
        1 actccacatt ggcaaaccgg tccacaccct gtgccgcaaa ctgccgtgcc aggtcctgct
       61 ggctggcact caggatgttg atggcgtagt gggagcccgc gctgaatgcc gccatcgagc
      121 ctgctgtgcg ggccagactc cacaacacca gcgggggcga catggaaacc gagttgaacg
      181 aattggccgt cagaccgacc agactgcctt gcgcggtccg cgcagtaacg atggtgacgc
      241 ccgtggcaaa catgcccaac gcggagcgga actccgggag cgagaaattg ggggggtgag
      301 cctggcgttg ccgcacgggt gaaagcatcg gacgaattta accgaatttc agggcagcag
      361 catgtcaccc gtgtgttcgt ccggctaacg cctgaccaac ctacaatggg cccatgttcc
      421 tgcgaatctg catttcagcg gtttttgcta tgctttccgt agctgctcat gcagatacga
      481 cgggggcttg caaggagttt cctttaaaac tcccgaatgt gcggccggtt ttgtgcgaat
      541 ccgcgggttt gcgcgacatg caggcgcgtt cggtgcaggg gcgagccatc tatggtcggg
      601 atatccagcc ggacgatgcc cgcttgcgcg tgctggtgat cggcggcatc cacggcgacg
      661 aaatgtcgtc cagtgccctg gtgttccact ggatctccct ggccagcgta ccgctgtccg
      721 acacccagct cccggtgcac tggcgcttca ttccggcgat caatcctgac gggctgtttg
      781 ccagacccgc acggcgcacc aatgccagcg gggtggatct caaccgcaat ttccccacgc
      841 ccaactgggc acgggacgct cgcgtctatt gggaaaaccg cacccgcaag gacccacggc
      901 gctggcccgg gaacaagccc ctgtcggagc cggagtcgcg tttcctgcat gaacagatga
      961 acacgttcaa gcccaacctg atcgtcagcg tgcacgcccc ctacggtgtg ctcgacttcg
     1021 atggcccctc ggttccacca agccggctcg ggcgcttgta tctggaccag gtagggattt
     1081 tcccggggtc actgggcaac tacggtggca tccacaagcg ggtaccggtg gtcaccatcg
     1141 agttgcccca tgccctgcgc acgccgcagg acgccgagat ccgccagatg tggatcgatt
     1201 tgatgcgctg gaccgacgaa aagctggggc cacggctggt ggctgcgtca ccctagttac
     1261 ccagtgtctg cggtgggttg ctgccctttc ccgcgggact gcgtggtacc gtttggggca
     1321 tgaaaatcat cgatgttgcc attgtgggtg ccgggtttgg cgggctgtgt gctgcgatac
     1381 ggttgcgtga gagtggcgtg gacaacgtgg ccattctgga gcgcagtggc gaagtgggag
     1441 gcacctggcg cgacaacgtc tacccggggt gcgcctgcga tgtgccctca cacatgtatt
     1501 ccttttcgtt tgagcccaat acgcgctgga cacgccccta tccgcagcag cccgaaattc
     1561 aggactacat cctgcggctg acggacacct accgcctgcg cccgctgatc cgctttcaca
     1621 gcgatgtgcg cagcatgcgg tggctggcag aacagaactg ctgggaactg gcggtggagg
     1681 gccagccccc tctgctggcg cgccatgtgg tgctggccac cgggccgctg agcaagccgg
     1741 ccattcctga tcttccgggt ctgggcagtt ttgccggcga aaccttccat tcaggccatt
     1801 ggcgccatga cgtgaacctc aagggcaagc gtattgccgt cgtaggtacc ggcgccagtg
     1861 ccattcagtt tgtgcccgag attgcgcccg atgccgcgca cgtggacgtc ttccagcgca
     1921 ccgctgcatg ggtaatgccc cgctgggaca aaccttacgg tgccgcccgg ctctggctgt
     1981 accgccatgt gccgggcctg cagcggctga gccgatggcg cgtctactgg ttcaacgaga
     2041 tggtgggcat gggttttatg ggctccagac gcgtgcagaa catgctgcgc aagctgtcgg
     2101 gtcaccatct gcaacgccag gtggcggacc cagccctgcg ccaggccctg accccggact
     2161 tcaacccggg ctgcaagcgc ctgctgatct ccaatacctg gtttcccacc ctgcagcggc
     2221 ccaacgtgca gttggtgacg cagggcgtgg cacgcattgc gccagagggt gtggtcggtg
     2281 ccgacggtgc gctgtacccc tgcgatgtga tcgtgtgggg cacaggcttc aaggccaccg
     2341 agtttgtggc gccgatgcag gtctatggcg aatcggccaa cggcgagccg ctggaattgg
     2401 gcaagctctg gggcgcgcag ccagccgcca cgcggctggg catcacggtg gcaggctttc
     2461 ccaacctgtt tctgctggtc gggcccaaca ccgggctggg ccacaactcc atcatcttca
     2521 tgatcgagtg ccaggtgcac tacatcgtca aagccctgca gtctttgcgc gccagcggca
     2581 agaatgcttt gcggctgcga cccgacgttc agcgcgacga ctatgccgtg atgcagcaga
     2641 agatgaagag cacggtgtgg tcctccggct gcaagagctg gtaccagaac gcccagggcg
     2701 agatcgacac cctgtggccc ggctacacgt gggaatactg gctccagacg cgaaggttca
     2761 aggccagcga ttacctctag aagcgcactc tggggctcag tgccccagga tcttggaaag
     2821 gaagtcccgg ctgcgttcgc tctgcgggtg gttgaagaaa tcgtgcgggt tgttctgctc
     2881 gacgatctgg ccctggtcca tgaagatcac gcggtcggcc acggccttgg caaagcccat
     2941 ctcgtgcgtg acgcagatca tggtgatgcc ctggccagcc atctcgatca tcacgtccag
     3001 cacctccttg atcatctcgg ggtccagcgc gctggtgggc tcgtcgaaca gcatgatctt
     3061 gggcgtgagg cacagggcgc gcgcgatcgc tacgcgctgc tgctgaccgc ccgagagttg
     3121 cagcgggtac ttgctggcct gctcggcaat gcgcacgcgc cggagctgct ccatggcttt
     3181 ttcttcggct tcctttttgg gcaatttgcc tacccacatg ggcgacagcg tcaggttttc
     3241 cagcacggtc aggtgcggga acaggttgaa ctgctggaac accatgccga ctttcttgcg
     3301 cacgtcgtca atggctttca catcgtcggt cagttccacg ccgtccaccg tcaggtggcc
     3361 ctgctggtgc tcttccagac ggttgatgca acggatcagg gtggacttgc ccgagcccga
     3421 ggggccgcag acgacgatgc gctcgccctt ggtgacggtg aggtcaatgt cgcgcagcac
     3481 gtgaaaattg ttgccgtacc acttgttgac tttgtcgaag gtgatgatgg gttctgacat
     3541 ggaaaatcct tagcggagct tgctcttgtt gacctgcgcc tcgatccact ggctgtagcg
     3601 cgacatggag tagcagaaca caaagtaaat gagggcgatg aacaggtagc cttcaatgcg
     3661 gaaggggcgc cagttggcat ccgagttcat ggccatgccc atggcgccgg tcagttcgta
     3721 caggctcacg atggccacca gtgaggtgtc cttgaacgtg gaaatgaagt tgttcatgat
     3781 gcccggcacc accatggcca gggcctgggg cagcacaatc ttgcgctggg tctgccagta
     3841 ggagaggccc agcgtggccg cggcttcaat ctgccctttg ggaatggcct gcaggccgcc
     3901 gcggatcacc tcggccatgt aggccgcggc aaacagcgtg atgccagcca gcacccgaat
     3961 cagcacgtcg atgttgaagc cctgcggcat cagcagcggg aacatgaatg acgccatgaa
     4021 cagtaccgag atcaggggca caccacggat cagctccaca tacacggtgc acaggctgcg
     4081 gatggccggc atggccgagc tgcgccccag cgcgatgaac aaggcaatgg gaaacgccat
     4141 gaccatggac agcgaggcca gcaggatcgt cagcggcagg ccactccaca ggtcggtctc
     4201 gacccgctcc agacccagca cgccgccacg catcagcgtg aaataaacca ccaacaccac
     4261 ggcccagagc gcacccagcc agggtttcca gaaggcgcgg gtgcaactgg ccatgatcag
     4321 cgccagcagc atcacggtac agatcagggc acgccagtgt tcgtcaaacg ggtagcggcc
     4381 aaacaggatg aagcggaatt tttcgcgcac cacaccccag caggcgccgc tggcatcgtg
     4441 gcaggcctgg tagtcggcta cccacacggc atcgatcagc gcccactgga ccaggcgtgg
     4501 cagcagcgcg agcagcaccc cgccgacgag gagggtgccc agcgtggtct gccagccgct
     4561 gaacaaattg atcttgcacc aggccagggc gccttcggtg cgcaccggtg ccgggcgcgg
     4621 gggaatgggt tgaaacgtct ttgaggtcat ggcgtgtcct taacgttcct tgatggccgc
     4681 gcgcgcgttg taccagttca tcagcagcga ggtcgacagc gatgtgctca ggtacaccag
     4741 catgatgatg gcaatgcact ccaccgcgcg gccggtctgg ttgatggcgg tgttggcaat
     4801 cgacactaca tcggggtagc caatggccac cgccagcgat gaattcttgg tgaggttgag
     4861 gt
//
LOCUS       DAOSJM010000176         4843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15599_length_4843_cov_3.695489, whole genome shotgun sequence.
ACCESSION   DAOSJM010000176 DAOSJM010000000
VERSION     DAOSJM010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15599_length_4843_cov_3.695489"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="PKX82_07365"
     CDS             complement(<1..1500)
                     /locus_tag="PKX82_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(methylthio)propionyl-CoA ligase"
                     /protein_id="HOF52110.1"
                     /translation="MLGLMQSHQLMISSLIEFAERHHGEAEVVSRRVEGDIHRYTYKD
                     VAVRSRKVANALEKLNLAFSDRVATLAWNGYRHLELYFGVSGSGKVLHTLNPRLHPDQ
                     IVWIANHAEDQVVCFDMTFLPIIKAVHSRCTTVKHWIALCDADKLPSDTGIPGVQSYE
                     AWIGAASSEYTWPEFDENSASSMCYTSGTTGNPKAALYSHRSTMLHAFAGALPDALNM
                     SARDAVLPVVPMFHVNAWGLPYSAAMTGAKLVFPGPAMDGKSIYDLIESEKVSFAAGV
                     PTVWQMMLSHMQAGNLKFSTLNRTVIGGSACPPAMITAFNDQYGVEVLHAWGMTEMSP
                     LGTVCTLKNKHITMSAEEKMKVRLKQGRGIFGVDLKIVDGAGESLPWDGKAYGDLLVK
                     GPWIIKEYFKGEGGAPLVDGWFPTGDVATIDPDGYMQITDRSKDVIKSGGEWIGSIDL
                     ENIAMAHPAVAMAACIAAFHPKWDERPLLVVMKKPGMDVTREELLAFYDG"
     gene            1788..2495
                     /locus_tag="PKX82_07370"
     CDS             1788..2495
                     /locus_tag="PKX82_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF52111.1"
                     /translation="MFPGTASPTARFLAAQTWFACLPLADQERVAGSLLHHTGRRGDV
                     MLLADSPVEGWYAVLSGLVKLQTQSVNGRSSTFLCAACGDWFGEGSAINTERRRYDVV
                     ALRDTELICLPDAEFHRLRASSLEFNQFLVSQLNLRVSQAMALIESARLRTPEQRVAL
                     SLSKLFWGRTRKLNLSQDDLAALAGVSRQTANQALHALAERGLVVLDFGRVDIPDDEA
                     LTRYIFSDTVTRQIQPA"
     gene            2600..4042
                     /locus_tag="PKX82_07375"
     CDS             2600..4042
                     /locus_tag="PKX82_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HOF52112.1"
                     /translation="MPWDNSFFHLGEDFHVPLAPVPLPDPYWVGRSALVARDMGLSTA
                     QMESHALLLAVSGNRKIAGTTPLASVYSGHQFGVWAGQLGDGRTLLLGEAAGREMQLK
                     GAGLTPFSRMGDGRAVLRSSIREFLCSEAMHGLGIPTTRALCVVGSDAPIFRETRETA
                     AVVTRVAPSFIRFGHFEHFCHHDQHAQLKVLADYCLDRLYPACREDVRFDGNPYAALL
                     AEVTARTAELIAQWQSVGFCHGVMNTDNMSILGLTIDYGPFQFMDAYDPAHVCNHSDT
                     QGRYAFQKQPHVAYWNLFCLGQALMPLIDVQENALAALEPFKDIYARAFARRMSAKLG
                     FAEPVDACKPLTEDILALLAADRVDFTIFWRRLSHWVRERRTDDDSVRDLFLNRSAID
                     AWLLSYSELLAQYGRGLEADLMVKTNPRYVLRNHLGELAIRAAQAKDFSVVANLLAVL
                     ERPFDEHPEHAAFADFPPDWAQGISVSCSS"
     gene            4068..4475
                     /gene="msrB"
                     /locus_tag="PKX82_07380"
     CDS             4068..4475
                     /gene="msrB"
                     /locus_tag="PKX82_07380"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOF52113.1"
                     /translation="MTYPVEKSDAEWKALLAQKGAESVAFEVTRHAATERPFSGKYET
                     HWADGSYHCMCCGAKLFNSDTKFDAHCGWPSFSLAEPGAIREITDRSHGMVRVETVCA
                     QCGAHLGHVFEDGPTPTGLRYCMNSASLHFTPE"
     gene            4472..>4843
                     /locus_tag="PKX82_07385"
     CDS             4472..>4843
                     /locus_tag="PKX82_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HOF52114.1"
                     /translation="MKILIDFFPLLLFFGAFKFYDIYVGTAVLMGATVVQMALIYGID
                     RKLQAMHKITLVLVLAFGTLTLVLHDDRFIKWKPTVLYAAMSIALAIAVWGLRKNFLK
                     MLLGSQLELKDAVWHRLNVAWI"
BASE COUNT          929 a         1473 c         1413 g         1028 t
ORIGIN      
        1 gccgtcgtag aaggccagca actcttcacg ggtcacgtcc atgccgggct tcttcatcac
       61 caccagcagc gggcgctcgt cccacttggg gtggaaggcg gcgatgcaag cggccatggc
      121 cacggcaggg tgggccatgg cgatgttctc caggtcgatg gagccgatcc attcaccgcc
      181 agacttgatc acgtccttgc tgcggtcggt gatctgcatg tagccatcag gatcaatggt
      241 cgccacgtca ccggtgggaa accagccatc caccagcggc gcaccgccct cccccttgaa
      301 gtattcctta atgatccagg ggcctttgac cagcaggtcc ccataggctt ttccatccca
      361 gggcaacgat tcgccggctc cgtcgacgat cttcaagtcg acgccaaaaa ttccacgacc
      421 ctgcttgagg cggaccttca ttttttcctc agcactcatg gtgatgtgct tgtttttcag
      481 cgtacaaacg gtgcccagcg gcgacatctc ggtcatgccc catgcgtgca gcacttcaac
      541 accgtattgg tcattgaagg ctgtaatcat ggctggcggg caggcggagc ccccaatcac
      601 cgtgcggttg agcgtagaga atttcaagtt gcctgcctgc atgtgcgaca gcatcatttg
      661 ccacaccgtg ggtacgccag cagcaaagct caccttctca ctctcgatca ggtcataaat
      721 ggatttgccg tccatggccg gtcccggaaa gaccagtttc gcgcccgtca tggctgccga
      781 atacggcagg ccccaggcgt tgacgtggaa catgggcacc accggcagca cggcgtcccg
      841 tgcgctcatg ttgagcgcat cgggcaaagc acccgcgaat gcatgcagca tggtcgagcg
      901 gtggctgtac aacgcagcct tggggttgcc ggtggttcca ctcgtgtagc acatgctcga
      961 cgccgagttc tcgtcgaact ccggccatgt gtactccgac gatgcggcgc caatccatgc
     1021 ttcgtagctc tgtactccgg ggatgccggt gtcggatggc agcttgtcgg catcacacag
     1081 ggcaatccag tgtttcaccg tggtgcaacg actgtgcaca gccttgatga tgggcaggaa
     1141 ggtcatgtca aagcacacga cctggtcttc tgcgtggttg gcaatccaga cgatctggtc
     1201 aggatgcagg cggggattga gggtgtgcaa aaccttgccg ctgccgctca cgccgaaata
     1261 cagttccagg tgccggtagc cgttccaggc cagggtggca acgcggtcgc tgaatgccag
     1321 attgagtttt tccagcgcgt tggccacctt gcgtgagcgc accgcgacat ccttgtaggt
     1381 gtaacggtga atatcgcctt cgacccggcg ggaaaccact tcagcctcgc catggtggcg
     1441 ctccgcaaat tcaatgagcg acgaaatcat caactgatgg ctctgcatca aacctagcat
     1501 ggaatctcct tcataaaaat tgtcttcact gtccatcgta gcggcgttca agagcgccac
     1561 tgcagttaac cttatttacc agcgctgatg atagtgattt caccgaagcg atttcaggca
     1621 gagctgcagc atgtgctcct gagcatcggg cgattgtgaa atcggcgggc aaacccggca
     1681 agaaatgtct atccggcgac aggttgtcgt cggctctaca atttctaggt atttacccga
     1741 gtgcatcgca tctttttcga cttcgcaata cggccctaat tccgcgtatg tttcccggca
     1801 ctgcctcacc gaccgcacga tttcttgctg cgcaaacctg gtttgcgtgc ctgccgcttg
     1861 ccgaccagga gcgtgtggca ggaagcctgt tgcaccacac cggccggcgt ggtgacgtga
     1921 tgttgttggc agattctccc gtggagggct ggtatgcagt actttccggt ttggtgaagt
     1981 tgcagacgca gtcggtcaat gggcgttctt cgaccttttt gtgtgcggcc tgcggcgact
     2041 ggtttggtga agggtctgca atcaacaccg agcgcaggcg ctacgacgtg gttgcgctgc
     2101 gagacaccga attgatatgt ctgcccgatg ccgaatttca ccggttgcgt gcgagcagcc
     2161 tggaattcaa ccagtttctg gtgagtcagc tcaacctgcg cgtcagccag gccatggccc
     2221 tgatcgaatc ggcccggctg cgtacacccg agcagcgtgt ggccctgtca ttgagcaagc
     2281 tcttctgggg gcgtacccgc aaactgaatc tgtcccagga tgatctggcc gctctggcgg
     2341 gtgtgtcgcg ccagaccgcc aaccaggcgc tgcacgcgtt ggccgaacgc ggtctggtgg
     2401 tactggactt tggccgggtg gatataccgg atgacgaagc gctcacccgc tacattttta
     2461 gtgacaccgt tacacgccag attcagccag cctgaccgca actgaacaga taaccctacg
     2521 gcccctggcc atcgccgtac tgccggacaa tcgaccgatg actatggcca cacacagcca
     2581 gggcgatatc gatcttggaa tgccctggga caacagtttt ttccacttgg gcgaagattt
     2641 tcatgtgccg ctggcgcctg tgccactacc cgatccctat tgggttggac gcagtgcgct
     2701 ggttgcgcgg gatatgggtc tgagcacagc ccaaatggag tcgcacgcgc tgctgctggc
     2761 cgtatcgggc aaccgcaaaa ttgccggcac cacaccactg gccagcgtct acagcggcca
     2821 ccagtttggt gtttgggctg gacagctggg tgacggccgg acgttgctgc tcggagaagc
     2881 cgcggggcgt gaaatgcagc tcaagggcgc cggactgaca cccttctcac gcatgggcga
     2941 tggccgggcg gtgctgcgca gcagcatccg ggagtttctc tgcagcgaag ccatgcacgg
     3001 tctgggcatc cctaccacgc gcgccctgtg tgtcgtcggt tccgatgccc ccatctttcg
     3061 cgaaacccgg gagacggccg cagtggtgac ccgcgtcgcc cccagtttca tacgctttgg
     3121 tcattttgag catttctgcc accacgacca gcacgcacaa ctcaaggtgc tggcggacta
     3181 ttgtctggac cgtttgtacc cggcgtgtcg cgaggacgtg cgttttgacg gcaacccgta
     3241 tgccgcgttg ctggcggaag tcactgcacg caccgcagaa ctgatcgcgc aatggcagtc
     3301 cgtgggtttt tgccatggcg tgatgaacac cgacaacatg agcatcctgg ggctgaccat
     3361 cgactacggc ccgttccagt tcatggatgc ctatgacccg gcccacgtct gcaaccactc
     3421 ggacacccag ggccggtatg ccttccagaa gcaaccccat gtggcctact ggaacctgtt
     3481 ctgcctgggg caggcgctga tgccactcat cgacgtgcag gagaacgccc ttgccgcact
     3541 ggaacccttc aaggacatct acgcccgtgc gtttgcacgc cgcatgtcgg ccaaactcgg
     3601 ttttgccgag cccgtggatg cctgcaagcc gctgacggag gacattcttg cgttgctggc
     3661 ggcagaccgc gtggacttca ccatcttctg gcgccgcttg agccactggg ttcgcgagcg
     3721 ccgcaccgac gacgattccg tgcgggacct gtttctgaac cgatcggcaa ttgatgcctg
     3781 gttgctatct tattcagagc tgctcgcgca atacggacgg gggctagagg ccgatttgat
     3841 ggtaaaaacc aacccgcgct atgtgctgcg caaccacctg ggggagctgg ccatacgggc
     3901 cgcccaggcc aaagacttct cggtggtggc caatttgctg gccgtcctgg agcgtccctt
     3961 cgacgaacac cccgaacatg ccgccttcgc cgattttccg cccgactggg cacagggcat
     4021 ttccgtgagc tgcagctcct gaacccttca tcttctaagg aaaacccatg acctatcccg
     4081 tcgaaaaatc ggatgctgaa tggaaggccc tgctggcgca gaaaggcgcc gaatcggtgg
     4141 cttttgaagt aacgcgccac gccgccaccg agcgcccgtt cagcggcaag tacgaaaccc
     4201 attgggccga tggaagctac cactgcatgt gttgcggcgc caagctgttc aactccgata
     4261 cgaagtttga cgcccactgc ggctggccca gcttttccct cgcagagcct ggtgccatcc
     4321 gggaaatcac tgaccgcagc cacggcatgg tccgcgtgga aacggtttgt gcccagtgcg
     4381 gggcccactt ggggcacgta ttcgaggacg ggcccacacc aacgggcctg cgatactgca
     4441 tgaactccgc ctcgctacac ttcacccctg aatgaaaata ctgatcgact ttttcccgct
     4501 gctgctgttc ttcggcgcct tcaagttcta cgacatctac gtgggtaccg ccgtgttgat
     4561 gggggccacc gtggtccaga tggcgttgat ctatggcatt gaccgcaaac tgcaggccat
     4621 gcacaagatc accctggtgc tggtgctggc gtttggcacg ctgacactgg tactgcacga
     4681 tgaccgcttc atcaaatgga agcccacggt gctgtacgcg gccatgtcga tcgccctggc
     4741 cattgcggtg tggggcctac gcaagaattt tctcaaaatg ctgctgggca gccagctcga
     4801 actcaaggac gccgtgtggc accgcctgaa cgtggcctgg att
//
LOCUS       DAOSJM010000177         4830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15646_length_4830_cov_5.978639, whole genome shotgun sequence.
ACCESSION   DAOSJM010000177 DAOSJM010000000
VERSION     DAOSJM010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15646_length_4830_cov_5.978639"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..871
                     /locus_tag="PKX82_07390"
     CDS             74..871
                     /locus_tag="PKX82_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF52115.1"
                     /translation="MSTMPPAISDQPVLKLLNVESSYGPIKAIRGVSLQVRRGEIATV
                     LGSNGAGKTTILKTISGIIDPRKGSIEFKGEIITAKDPAFIVQQGLSHVPEGREVFPL
                     LSVHDNLLMGAYTRSDRDGVARDMETVFGYFPILKERSAQDAGLLSGGQQQMLAISRA
                     LMADPDLILLDEPSLGLSPKLTKEIFEIVVRINRERGTTILLVEQNANMALNASDYGY
                     VLENGRIVMEDTCARLREKDDIKEFYLGLKDDGVRGERRWKKKKTWR"
     gene            877..2733
                     /locus_tag="PKX82_07395"
     CDS             877..2733
                     /locus_tag="PKX82_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOF52116.1"
                     /translation="MSGLWDTNNMQPNHDIVVQGETIPAIFWNAVEKRGPGIWMRQKH
                     LGLWRSWTWNQTADAVREIAAGLVSLGFNPGDCASVLANTVVEWVWADLAILSCAGIS
                     NGVYPTDAASQLQYLCEDSRTTILFVEDDEQLDKALEVRDQLPLLRKIVVFDMEGLRG
                     LDEPDVISLDLLRSLGRTYLASHPNAVTDRVAACKPDDIAILVYTSGTTGKPKGAMHL
                     HSGIVFTLRGFNTLVAQDERDERMCFLPLCHIAERMGGEYFALYTGTKLNFVENPETI
                     PENVREIAPTVFTAVPRVWEKFYSGVMISLKEAGAIQQAAYAWGIGVGTTIANKVLAG
                     EPVGAWLKLKFTVAQWLALNNVRKLIGIHRARFLVTGAAPISPDLVRWYLALGVPMLE
                     VWGMTETCGASTGVPVAKMKPGSIGPAAAFNEVRLDPVTGEILVRGKNVFAGYLNLPE
                     KTAETIDKDGWLHTGDVGVVDADGYFRITDRMKDIIITAGGKNITPSELENELKFSPY
                     ITDAVVIGDKRAYLTVIVMIDQENVEKFAQDQDVPFSNYASLTKAPEVQALIQGELDR
                     VNKKFARVEQIKKFFLLDTQLTAEDEELTPTMKLKRKLVEKKYSPQIEAMYR"
     gene            2765..3928
                     /locus_tag="PKX82_07400"
     CDS             2765..3928
                     /locus_tag="PKX82_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF52117.1"
                     /translation="MKLNTALTLIALATAVNLHAAEQQGVSKTEILIGTIQDLSGPLA
                     GYGKQARNGMQLRVDEINEQGGVNGRKLKLLVEDSGYDPKKAVLAAQKLVNQDKIFIM
                     AGHLGTAHNNAAMPVQFEKRVVNFMPLTAAREMYEPFNPLKYALLSSYYDQMRTTLPK
                     MVKDKSAKKVCAIYQDDEFGLEVLRGAEAGLKTMGMDFVEKTSFKRGATDFSSQVAKM
                     KSAGCDMVVLGTIIRETIGTIGESRKNGFNPVFFGSQAAYTDLIHKLGGKAMDGMYAT
                     MSVQNPYLDEASQPIRFWANKYKTRFNEDPTVFSAYGYTMIDLFIQAAQKAGPNLTTD
                     SFIKTMDSLTVPPDMFGAPQLKYTSKQHLGSDLSRLSQIQDGKWKVVSEYVKP"
     gene            3979..>4830
                     /locus_tag="PKX82_07405"
     CDS             3979..>4830
                     /locus_tag="PKX82_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF52118.1"
                     /translation="MKMKTAIALASLALTATLAHSQQGVSKDEITLGSIQDLSGPIAG
                     FGKQARLGMMLAVDEINEQGGVNGRKLKLIVEDSAYDPKKAVLAAQKLVNQDKIFMMV
                     GHIGTAQNMAAMPVQFEKNVINFFPITAAREMYEPFHKLKYSYAATYYDQMRNAVPKL
                     VKEKGAKKACVMYQDDDFGLEVLKGGEAGLKTLNMEFAEKTSYKRGATDFSSQISKMQ
                     AGGCDMIVLGTIIRETIGAIGTARKLGYNPIFLGSSAAYTDLIHKLGGKAMDGLYATM
                     TVQNPYLD"
BASE COUNT         1102 a         1407 c         1430 g          891 t
ORIGIN      
        1 agaccgatcc gggcgtggtg gaagcctacc tgggctcggt ggacgatgtg tcgtcattgc
       61 gcagggaggc cgcatgagca ccatgcctcc tgccatcagc gaccagcccg tgctgaagct
      121 gctcaacgtc gagagctcct atggcccgat caaggcgatc cgcggcgtga gcctgcaggt
      181 acgccgcggc gagattgcca ctgtgctggg ctccaacggc gcgggcaaga ccaccattct
      241 caagaccatt tcgggcatca tcgatccgcg caaggggtcg atcgaattca agggcgagat
      301 catcaccgcc aaagacccgg cattcatcgt gcagcagggg ctgagccatg tgcccgaagg
      361 gcgcgaggtg ttcccgctgc tgagcgtgca cgacaacctg ctgatgggcg cctatacccg
      421 ctcggaccgc gatggcgtgg cgcgtgacat ggaaaccgtc tttggctact tcccgatcct
      481 gaaggagcgc tctgcacagg atgcgggcct gctgtctggc ggtcagcagc agatgctggc
      541 gatttcgcgc gcactgatgg ccgacccgga cctgatcctg ctggacgagc ccagcctggg
      601 cctgtcgccg aagctcacca aggagatctt cgagatcgtg gtgcgcatca accgcgagcg
      661 gggcaccacg attctgctgg tggagcaaaa cgccaacatg gcgctcaacg cctccgacta
      721 tggctatgtg ctggagaacg gccgcatcgt gatggaagac acctgtgccc gtctgcggga
      781 gaaggacgac atcaaggagt tctacctggg cctcaaggac gatggcgtac gcggcgagcg
      841 gcgctggaaa aagaagaaga cctggagata gacaacatga gcggactctg ggacacaaac
      901 aacatgcagc ccaaccacga catcgtggtg cagggcgaga ccattccggc catattctgg
      961 aatgcggtgg aaaaacgcgg ccccggcatc tggatgcggc aaaagcacct gggcctgtgg
     1021 cgcagctgga cctggaacca gactgccgat gcggtgcgcg aaattgccgc cggactggtg
     1081 agtctgggtt tcaacccggg cgactgcgct tcggtcctcg ccaacacggt ggtggaatgg
     1141 gtgtgggccg atctggcgat tctttcctgc gcaggaatat ccaacggtgt gtatccgacg
     1201 gatgcggcct cgcagctgca atacctgtgc gaagactcgc gcaccaccat tctctttgtg
     1261 gaagacgacg agcaactgga caaggcgctg gaagtgcgcg accagttgcc gctgctgcgc
     1321 aagattgtgg tgtttgacat ggaaggtttg cgcggcctgg atgaacccga cgtcatcagt
     1381 ctggacctgc tgcgaagcct gggacgtacc tacctggcat cgcaccccaa cgcggtgacc
     1441 gaccgggtgg ccgcctgcaa gcccgatgac atcgcgattc tggtctacac ctccggcacc
     1501 accggcaagc ccaagggggc catgcacctg cacagcggca tcgtgttcac tttgcgtggt
     1561 ttcaacacgc tggtagccca ggatgagcgc gacgagcgca tgtgcttcct gccgctgtgc
     1621 cacattgccg agcgcatggg cggcgaatat tttgcgctgt acaccggaac caagctgaac
     1681 tttgtggaga accccgagac cattcccgaa aacgtacgcg agattgcgcc aactgtcttc
     1741 actgcagtgc cccgcgtctg ggaaaagttt tactcgggtg tgatgatttc cctgaaagag
     1801 gccggtgcca tccagcaggc agcctatgcc tggggcattg gcgtgggaac gaccattgcc
     1861 aacaaggtgc ttgcgggcga gccggtcggg gcctggctca agttgaagtt cacggtagcg
     1921 cagtggctgg cactgaacaa cgtgcgcaag ctcatcggca tccaccgggc gcgttttctg
     1981 gtgacggggg cggcccccat ctccccggat ctggtgcgct ggtatctggc actgggcgtg
     2041 ccgatgctgg aagtgtgggg catgaccgag acctgcggtg catccaccgg tgtacccgtg
     2101 gcgaagatga agcccggctc cattggcccc gccgcggctt tcaacgaagt gcggctggac
     2161 ccggtgaccg gcgaaattct ggtacgtggc aagaacgtct ttgccggtta tctgaacctg
     2221 ccggaaaaga cggccgagac catcgacaag gacggatggc tgcacaccgg tgatgtgggg
     2281 gtggttgacg cggacggata tttccgcatc acggaccgca tgaaggacat catcatcacc
     2341 gccggcggca agaacatcac gcccagcgag ctggagaacg agctgaagtt ctcgccctac
     2401 atcaccgatg cggtggtcat tggtgacaag cgtgcttacc tgactgtgat cgtcatgatc
     2461 gaccaggaaa acgtcgagaa atttgcgcag gaccaggatg tcccgttcag caactacgcg
     2521 tctctgacca aagcgcccga agtccaggcc ctgatccagg gcgagctgga ccgcgtgaac
     2581 aagaagtttg cacgcgtgga acagatcaag aagtttttcc tgctggatac ccagctgacc
     2641 gctgaggacg aagaactcac gcccaccatg aagctcaaac gcaagctggt ggaaaagaag
     2701 tattcgcccc agattgaagc gatgtaccgc taaccccgat atcccctcaa actcccagga
     2761 ccccatgaaa ctcaataccg cactcacctt gatcgctctg gccacggccg tcaatttaca
     2821 tgctgccgag cagcaaggcg tcagcaaaac cgaaattctc attggcacca tccaggactt
     2881 gtcgggtccg ctggcgggct acggcaagca ggctcgcaat ggcatgcaac tgcgtgtcga
     2941 tgaaatcaac gagcagggtg gcgtcaatgg ccgcaagctc aagttgctgg tggaagactc
     3001 tgggtacgac cctaaaaagg ccgttctggc agcgcagaag ttggtgaatc aggacaagat
     3061 cttcatcatg gcaggccatc tgggtacggc acacaacaat gccgccatgc ccgtgcagtt
     3121 cgagaaacgg gtggtcaact tcatgccgct gacggccgcg cgcgaaatgt acgagccatt
     3181 caatccgctg aaatatgccc tgctgagctc gtactacgac cagatgcgca ccaccctgcc
     3241 caaaatggtg aaggacaaga gtgcaaaaaa agtctgcgcc atctaccagg acgacgaatt
     3301 cgggctggag gtactgcggg gagccgaagc gggtctcaag accatgggca tggactttgt
     3361 tgagaagact tccttcaagc gtggtgcaac ggacttctcg tcccaggtcg caaagatgaa
     3421 gtctgcgggt tgtgacatgg tggtactggg caccatcatt cgtgaaacca tagggaccat
     3481 tggcgaatcg cgcaagaacg ggttcaaccc cgtgttcttc ggctcacaag ctgcctatac
     3541 cgacctgatt cacaagctcg gtggcaaggc catggatggc atgtacgcca ccatgtcagt
     3601 gcaaaaccct tacctggacg aggcatcgca acccatccgc ttctgggcca acaagtacaa
     3661 aacccgcttc aatgaagacc ctaccgtgtt ctcggcctat ggctacacca tgatcgacct
     3721 gttcattcag gcggcgcaaa aggcgggacc caatttgacg acagactcgt ttatcaaaac
     3781 gatggacagc ctgacggtac cacccgatat gttcggtgca ccccagttga agtacacatc
     3841 aaaacagcac ttgggtagcg acctgtcgcg cctttcgcaa atccaggacg gcaagtggaa
     3901 agtggtgtcc gagtacgtca agccctgatc catccgttct tttcgacccc gcgtttctca
     3961 cactttgagg agttcaccat gaagatgaag actgcaattg ccttggccag tctggcactc
     4021 accgctacgc tcgcacattc ccagcagggc gtcagcaagg acgaaatcac cctggggtcc
     4081 attcaggatc tttccggacc gattgccggc ttcggcaagc aggcacgtct cgggatgatg
     4141 ctggcggtag atgaaatcaa cgagcagggc ggcgtcaacg gccgcaagct caaactcatc
     4201 gtcgaagact cagcttacga ccccaagaag gccgtgttgg ctgcgcaaaa gctggtcaac
     4261 caggacaaga ttttcatgat ggtggggcac atcggaaccg cccagaacat ggcggccatg
     4321 ccggtgcagt tcgagaagaa cgtgatcaac ttcttcccga tcaccgcggc acgcgaaatg
     4381 tacgagcctt tccacaagct caagtactcc tatgcagcca cgtactacga ccagatgcgc
     4441 aatgccgtgc ccaaactggt caaggaaaaa ggtgcgaaga aagcctgcgt gatgtaccag
     4501 gatgacgatt ttggtctgga agtgctcaag ggcggcgagg ccggtctcaa gacgctcaat
     4561 atggaatttg ccgaaaaaac gtcttacaaa cgcggagcga cagacttctc ctcgcaaatc
     4621 tccaagatgc aagccggtgg ctgcgacatg attgtgctgg gcaccatcat ccgcgaaacc
     4681 atcggtgcca ttggtactgc ccgcaagctg ggctacaacc ccatcttcct gggctccagt
     4741 gctgcgtaca ccgacctgat ccacaagctg ggcggcaaag ccatggacgg cctgtacgcg
     4801 accatgacgg tgcaaaaccc ctacctggac
//
LOCUS       DAOSJM010000178         4759 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15935_length_4759_cov_4.873087, whole genome shotgun sequence.
ACCESSION   DAOSJM010000178 DAOSJM010000000
VERSION     DAOSJM010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15935_length_4759_cov_4.873087"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PKX82_07410"
     CDS             <1..317
                     /locus_tag="PKX82_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOF52119.1"
                     /translation="KVGQEHGVDVKIEMVSSYNAIAFHDNCVDAVGRAAKKLGYSNMP
                     AVSGAGHDAVYMAKLAPSGMIFIPCKDGISHNEIEDAKPEHITAGCNVLLHAMLERAG
                     VV"
     gene            881..1120
                     /locus_tag="PKX82_07415"
     CDS             881..1120
                     /locus_tag="PKX82_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HOF52120.1"
                     /translation="MSTMNISLPDGLKEFVDTQVGSRGFGTSSEYLRELIRKDQDRQQ
                     LRGLLLAGAQSAPTAPVDAAYFDSLRKRIGGKKSK"
     gene            1117..1449
                     /locus_tag="PKX82_07420"
     CDS             1117..1449
                     /locus_tag="PKX82_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOF52121.1"
                     /translation="MKVKALIQRTLALGDIDQAITHYLEQDAAAAALGFVDALERAFT
                     HICKHSATGSPRYAHELGIPDLRSWPLSKYPYLVFYVERADHIDVWRVLHMKRDIPAW
                     MMDSTDEK"
     gene            1517..1726
                     /locus_tag="PKX82_07425"
     CDS             1517..1726
                     /locus_tag="PKX82_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOF52122.1"
                     /translation="MQAWSVQDAKAKFSEFLDACLAEGPQMVTKRGAAVAVSRAAFPE
                     ATLAVQRGPYRTGHSSARQGTAPQS"
     gene            1827..1937
                     /locus_tag="PKX82_07430"
                     /pseudo
     CDS             1827..1937
                     /locus_tag="PKX82_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
     gene            1986..2303
                     /locus_tag="PKX82_07435"
     CDS             1986..2303
                     /locus_tag="PKX82_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOF52123.1"
                     /translation="MTIAVRDIVAFSDARTNLSRLVDEVRLGGEKIITKNGEPAVAMI
                     DAKRLDHYHRLEQDRVHLLLLDEVEKGLVDVVAGRTGDALEAIAAMQSQRSKAKRTGK
                     KAS"
     gene            2300..2674
                     /locus_tag="PKX82_07440"
     CDS             2300..2674
                     /locus_tag="PKX82_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOF52124.1"
                     /translation="MIAVRTTENFTANLQEIAAFWDGREYPAGFDALLAELSDPVIPN
                     LMRHPRMGRHFRLRHPQSVDSLARVQKMDAMLKTMGTLREAAEVREYVMTDYLLLYLL
                     ADTTIHLLAIKHQKQLAFDFTH"
     gene            2682..3320
                     /locus_tag="PKX82_07445"
     CDS             2682..3320
                     /locus_tag="PKX82_07445"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HOF52125.1"
                     /translation="MPVVRRPTCPHCQRPQSTCICRFVTPTPTACEVLILQHPLEEHH
                     AKNSARLLHLSLPGSRMVVGEAFDNATLQALMPQPKYTVLLYPPTEYEGHAAPALLDA
                     TQLVNLQYVRLVVLDATWRKSRKMLHLSPGLQRLPRLTLDDVPASRYAIRKAHKPGQL
                     STLEATCAALAQLEGDAARWQPLLHAFDGLVARVRSDFAGAVPRFALTERCE"
     gene            3317..3958
                     /locus_tag="PKX82_07450"
     CDS             3317..3958
                     /locus_tag="PKX82_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOF52126.1"
                     /translation="MTLSTWWLFILMTFVVSATPGPNMLYVMSISARHGIRAAVMAML
                     GCMTALLAMMSISAAGLGALLHTFPTVFDALRLAGAAYLAYLGVKCWRAPVHDEVADA
                     AGGASRAAAPAVQTSPIFRRGFLVAASNPKAILFAAAFFPQFINPGEPQLVQFAILLT
                     TFSVIEVAWYFVYAVSGKRLSVYLQRASVMRLFNRLTGGVFIGFAALMATARE"
     gene            complement(4014..4643)
                     /locus_tag="PKX82_07455"
     CDS             complement(4014..4643)
                     /locus_tag="PKX82_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOF52127.1"
                     /translation="MTHVADAPKGLIRQTNEALILSAAERVFARAGFGGATMATIAEA
                     SGLPKANLHYYFGSKEVLYRAVLARILSDWLVPTHGITADAEPRAAIEQYIRAKMALS
                     AQRPDGSKVFANELLHGAPVVHTLLSTELRQMVLQKAAVVQSWVDAGRMAPVDPIHLF
                     FTIWAATQTYADFDVQVSAVLGRKTLTAKDHARATEHVVSLILRGCGLL"
BASE COUNT          969 a         1420 c         1410 g          960 t
ORIGIN      
        1 ccaaagtcgg ccaggagcac ggcgtggacg tgaagattga aatggtgtcc agctacaacg
       61 ccatcgcctt ccacgacaac tgcgtagacg ccgtgggccg cgccgccaag aagctgggct
      121 acagcaacat gcccgccgtc tcaggcgccg gccacgacgc cgtctacatg gccaagctgg
      181 cccccagcgg catgatcttc atcccctgca aagacggcat cagccacaac gagattgagg
      241 acgccaagcc ggagcacatc accgcggggt gcaatgttct tctgcatgcg atgttggagc
      301 gggcgggggt ggtttgactg atcttcggaa ggggattaag agtcatattg acctctagcc
      361 cccgtattca ttgcgcgaac agctcctgat ttagtagcac gtaggggcta tttcattttc
      421 agagttgctc tcaccacatt cgactcggct attcaatccg cactaccgta gacaatctgc
      481 agcacaaagc aaaagggagg aatccatgtc tttggttgcc gtcagtgact agcaacacaa
      541 agaacgcgct gcacaaactg gcgactattg gtttgatcaa tgaagacctg ctgaagactt
      601 ggcagcattt gcggaacaaa tctacgcatg cagacggatt gcaaatgggc gactcccagc
      661 ttcaaatact tttagacgag attcatgctt gcttggagct tttctatcga ctgattttga
      721 gtcatcttgg ctacacagat tctttcgtgc gctattcagc atgcgtagga ggcctgagtg
      781 agttacccgt tgcccggctt ccacaaccgc agtaggcaat ttgatccaaa gaaattccag
      841 tattttggca aattttgcca aaatcactaa aattgcttgc atgagcacca tgaacatttc
      901 cctgcccgac ggcctcaagg aattcgttga cacgcaagtc ggctcgcgtg gctttggcac
      961 cagcagcgaa tacttgcgcg agctaattcg caaggaccag gaccgtcaac agttgcgggg
     1021 cctactgctc gcaggtgcgc agtcggcacc cacagcgccc gtcgatgctg cttacttcga
     1081 ctccctgcgc aaacgcattg gcggcaagaa atccaagtga aggttaaagc gctgattcag
     1141 cgcaccctgg cattgggtga catagaccaa gccatcacgc actacctgga gcaggatgca
     1201 gcggctgcag cgctgggttt tgtggatgcg ctggagcgcg cctttaccca tatctgcaaa
     1261 cattccgcca caggttcacc ccgctatgca cacgaactgg gtattcctga tctgcgctcc
     1321 tggcctttga gcaagtatcc ctatctggtt ttctatgtgg agcgtgctga ccacatcgac
     1381 gtatggcgtg tactgcacat gaagcgtgac attcctgctt ggatgatgga tagcacagac
     1441 gaaaaataga acgcatcttg acaacccgcc ttcagcgtac aaactagtca gttacccatt
     1501 cagatggagg cgcttcatgc aagcatggtc agtacaggat gcgaaagcaa agttcagtga
     1561 gttcttggat gcgtgcctgg ccgaagggcc gcagatggtg accaaacggg gggcggcggt
     1621 tgcagtcagc cgcgcggcct tcccagaagc aactcttgct gtccaacgcg ggccataccg
     1681 aactggtcat tcctccgcgc ggcaaggcac agcgccgcag agttgaagcg gcgcagtaac
     1741 agcccatgta tttgccggac accaatgtcg tcaacgctgg gctcggttaa gcacgcacct
     1801 ccaacacgtt gtaggtgttc accatgattg cagccaccgc agacgtgcac ggtttgacgg
     1861 tggtgacgcg caacgtggcc gatttcaaat ccttcggcgt tgcgctgctg aacccctttg
     1921 ccaagacagc tggttagcat attgcacaca attttgtaca atacacagaa acccggagcc
     1981 agcccatgac catagccgtt cgcgatatcg tggcgttttc agatgcccgc accaacttgt
     2041 cccgcctggt agacgaggtg cgactgggtg gggaaaagat catcaccaag aatggtgaac
     2101 ccgccgtggc gatgattgac gccaaacggc tcgaccacta ccaccgtctg gagcaagacc
     2161 gtgtgcacct tctgctgttg gacgaagtag agaagggttt ggtcgatgta gtggcaggac
     2221 gcaccggtga cgcgctggaa gccatcgccg ccatgcagtc acagcgcagc aaagcaaaac
     2281 gcactggcaa gaaagcatct tgattgcagt ccgcaccacc gagaatttca ccgccaatct
     2341 gcaggagatt gcggctttct gggacggcag ggaatacccc gcaggctttg atgccctgct
     2401 tgctgaactg agcgacccgg tcatccccaa cctgatgcgt caccctcgca tgggtcgaca
     2461 cttccggttg cgacatccgc agtccgtaga cagtctggca cgcgtccaga agatggacgc
     2521 gatgctcaag acgatgggaa ccctacgaga agctgcagaa gtgcgggaat acgtgatgac
     2581 ggattacctg ctgctctatc tgctcgccga tacgacgatt cacctgctcg caatcaagca
     2641 ccagaagcaa ctggcgtttg acttcacaca ctgagcgcac gatgccagtg gtacgccgcc
     2701 ccacctgccc ccactgccag cgcccccaat ccacctgcat ctgccgcttc gtcacgccca
     2761 cgccaaccgc gtgtgaggtg ctgatcctcc agcatccgct ggaagagcac cacgccaaga
     2821 acagcgcccg cctgctgcac ctgagcctgc caggcagccg catggtggtg ggtgaagcgt
     2881 ttgacaacgc gacgctgcaa gcactgatgc cacagcccaa gtacacggtg ctgctgtacc
     2941 cgcccaccga atacgaaggc cacgcggcac ctgcgctgct ggacgctacg caactcgtca
     3001 atctgcagta tgtgcggctg gtggtgctgg acgccacctg gcgcaagagc cgcaagatgc
     3061 tgcacttgag ccctgggctg cagcgcctgc cgcggctgac gttggacgat gtgcctgcgt
     3121 cgcgctatgc gatccgcaag gcgcacaagc cagggcagct ctccacactg gaggccacct
     3181 gcgcggcgct ggcgcagctg gagggggacg ctgcgcgctg gcagccgctg ctccacgcct
     3241 ttgacgggtt ggtggcgcgc gtaagatcag acttcgctgg ggcggtgccc cgttttgccc
     3301 taacggagag atgtgaatga cgctgagtac ctggtggctg tttatcctga tgacgttcgt
     3361 ggtctcggcc acgccgggcc ccaacatgct gtacgtgatg agcatcagcg cgcgccacgg
     3421 catccgcgcg gcagtcatgg ccatgctggg gtgcatgacg gccctgctgg cgatgatgag
     3481 catctcggca gcgggtctgg gcgcattgct gcacaccttt cccaccgtgt ttgacgcact
     3541 gcgcctggcc ggtgcggcct acctggcgta tctgggggtc aagtgctggc gtgcaccggt
     3601 gcacgacgag gtggcggatg cggcaggtgg cgcatcccgt gccgctgcgc ccgcggtgca
     3661 gaccagcccg attttccggc ggggctttct ggtggcggcc agcaacccga aggcgattct
     3721 gtttgcggcg gcgttctttc cgcagttcat caacccgggc gagccccagc tggtgcagtt
     3781 tgcgattctg ctcaccacgt tcagcgtgat cgaggtggcc tggtattttg tgtatgcggt
     3841 cagcggcaag cgcctgtcgg tctacctgca gcgcgccagc gtgatgcgcc tgttcaaccg
     3901 cctcacgggc ggcgtgttca tcggctttgc ggcgctgatg gccactgcgc gcgagtaatt
     3961 ttgatgaaaa cgggctctag cccccggaaa tagtgcgcaa gcagctatta tttttatagc
     4021 aatccgcagc cccgcaggat cagggacacc acatgttcgg tggcccgggc gtggtctttg
     4081 gcggtcagtg ttttgcggcc cagcacggcg ctgacctgca cgtcaaagtc ggcataggtc
     4141 tgggtggcgg cccagatggt gaagaacaga tggatggggt ctaccggtgc catgcgcccg
     4201 gcgtccaccc agctttgcac cacggcggct ttctgcagca ccatctggcg cagttcggtc
     4261 gacagcaggg tgtgcaccac cggtgcgccg tgcagcagtt cgttggcaaa caccttggag
     4321 ccatcgggcc gctgtgcgga cagcgccatc ttggcgcgga tgtattgctc aattgcagca
     4381 cggggttcgg cgtcggccgt gatgccgtgc gtgggcacca gccagtcgct gaggatgcgc
     4441 gcgagcaccg cgcggtacag cacttccttg ctgccaaagt agtagtgcag gttggccttc
     4501 ggcagtccgc tggcctcggc gatggtcgcc atggtggcgc cgccgaagcc ggcgcgcgcg
     4561 aacaccctct ctgcggcaga aagtatcagg gcctcattcg tctggcgaat gaggcccttg
     4621 ggagcatcgg caacgtgagt catgccgccg attgtccaat cacttctgtg ccgcttccca
     4681 gatggcgtgc gaatacgctg ccttgcccgg gtctttcttg atcacgggtg agctgccgcc
     4741 ggccagcaaa accttgacg
//
LOCUS       DAOSJM010000179         4757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15947_length_4757_cov_4.086134, whole genome shotgun sequence.
ACCESSION   DAOSJM010000179 DAOSJM010000000
VERSION     DAOSJM010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15947_length_4757_cov_4.086134"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="PKX82_07460"
     CDS             <1..333
                     /locus_tag="PKX82_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF52128.1"
                     /translation="IDDFGTGYSSLNYLKKFKVYKVKIDRSFVRDIAGDPDDRAIVSA
                     IISMSRSLGMQTIAEGVETAEQLAYLKAQGCGEVQGYYFSKPVPAEAFVQLLQKSIPM
                     DGSLELTP"
     gene            complement(346..1812)
                     /locus_tag="PKX82_07465"
     CDS             complement(346..1812)
                     /locus_tag="PKX82_07465"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF52129.1"
                     /translation="MPAHQFLLPVVARDEVVALVALGTDDRSGLPEQILEQLRDLGGR
                     VGVALAAHAREAQLVFMARHDGLTGLPNREFLSERMEQELAHARRDGSQLALLFIDLD
                     RFENINDSLGHDAGDQLLCQVAGRLKSSVRDSDTIARLGGDEFVVLQTGLQSPRHAAK
                     MAEGILQVLAQPFVIAGAEHFIGGSIGMAVYPDDGQTAEELLKRADIAMYRAKNAGRG
                     CLVFFEESMNQELQERTFLEHELRQAIARNQLSVHYQPRIRLRDGKVSGAEALVRWHH
                     PELGWISPARFIPLAEDTGLIDQIDPWVLSHVCTQMAAWKAAGHALGTIAVNVSGRQL
                     RATNLVDQVRDALASSGLAAKSLELEVTESVLINDVEFVIALLNQLREIGVSIALDDF
                     GTDYSSMMYLKRLPISILKIDQSFIKDLSHDEGSRSIAQAIIALAHALHKSVVAEGVE
                     TQAQADLLRSWGCEEVQGYYFSRPLPADAFEKVLGRGK"
     gene            complement(1782..3149)
                     /locus_tag="PKX82_07470"
     CDS             complement(1782..3149)
                     /locus_tag="PKX82_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOF52130.1"
                     /translation="MKLHIQGTFLRSAVARRVFFLFVISAFIPVAILGVLSYRHVFNV
                     VSESGQRQLSQAAGAYARTVYDHLLGASFILNADAAQIRVFTNGSSPRPANFESAFLR
                     VYRITHGKAAMRWGSAFASPPPQIPDALESHLARGEVALLPSHAVGSIPTLWLAVRID
                     TTQPERGELLAELDPKYLWDDPETLPFQTSICVLDQRSALLTCSDADLGATAVNASLR
                     GESGKTVEIDGWLTARSALFLKARFAQPEWTIIAMRPGGLAFESLTQMAQTLIGIAVA
                     TLLFVALLSVSQIRRTLVPLERLMEGTRRIESEQFDVPVSIARKDEFGQLADSLNTMA
                     SRLGQNINAMQALSRIDQQILSHIDIAQIIDRVQQRMLEILPHALVSVVVPDELNANL
                     GTVYLRKKGEHQSQQFRQPLENLRLSSLLPHAAGLWLDAASTAPPTRPPCLVNVCQPT
                     SFCCR"
     gene            complement(3146..3316)
                     /locus_tag="PKX82_07475"
     CDS             complement(3146..3316)
                     /locus_tag="PKX82_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52131.1"
                     /translation="MARKDHKFNPINSDYDLYSTGKNGVSKTQLTQKDSLDDIVRGRD
                     GQFVGLAGDFSP"
     gene            complement(3347..3622)
                     /locus_tag="PKX82_07480"
     CDS             complement(3347..3622)
                     /locus_tag="PKX82_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOF52132.1"
                     /translation="MLLNISRNRRHFGFTLVEVLIAVSLVGVLAAIAIPKYNNYRDRM
                     DQSKAITDIRILQTLITDYFASGGTYPASLADVGNANLLDPWGRPYV"
     gene            complement(3759..>4757)
                     /locus_tag="PKX82_07485"
     CDS             complement(3759..>4757)
                     /locus_tag="PKX82_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOF52133.1"
                     /translation="SNVRQGSEGVATASAEIAQGNHDLSSRTEHQASALQQTASSMDH
                     LGSTVKQNAENARQANQLALNASTVAIKGGDVVSQVVETMKGINDSSRKIADIISVID
                     GIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAGRSAEAAKEIKTLINASVER
                     VERGTTLVDEAGTTMAEVVGSIKKVTDIVGEISAASNEQAQGVTQVGDAVTQMDHATQ
                     QNAALVEEMAAAASSLKSQAQDLVQVVAAFKLNDADGAGSLPKVSVRPAAPKILPSQD
                     THRRALGTNGSPAQRAAASAKAAPPKRRPTTAAPAALPKPSPKPAPASGDDDWETF"
BASE COUNT          904 a         1367 c         1453 g         1033 t
ORIGIN      
        1 atcgacgatt tcggaacggg gtactcgtct ctgaactacc tgaagaaatt caaggtctac
       61 aaggtcaaga tcgaccggtc atttgtgcgc gatatcgcag gagatccaga cgaccgggcc
      121 attgtgagtg ccatcatttc gatgtccaga agcctgggca tgcagaccat cgccgaaggc
      181 gttgagacag ctgaacagct tgcctatctc aaagcgcagg ggtgtgggga ggtacagggc
      241 tactatttca gcaagcccgt gcctgccgaa gcgttcgtgc aactgctgca gaaaagcata
      301 ccgatggatg ggtcacttga gttgacgccc tgaacggtca cccgtttact tgccgcgacc
      361 cagaactttt tcgaatgcgt cggccgggag agggcggctg aaatagtaac cctggacctc
      421 ctcgcaaccc caggaccgca gcaggtctgc ctgtgcctgg gtttccacgc cctctgccac
      481 taccgattta tgcagtgcat gggccagcgc aatgatggcc tgcgcgatgc tgcgcgagcc
      541 ttcgtcgtgg gagaggtcct tgatgaagga ctggtcaatt ttgaggatgc tgatgggcaa
      601 ccgtttcagg tacatcatgg acgagtagtc ggtgccaaag tcatccagag cgatggatac
      661 gccgatttcc cgcaactgat tgagcaatgc gataacgaac tcaacgtcgt tgataagcac
      721 gctttcggtg acttccagtt ccagtgattt cgcagccaga ccggagctgg caagtgcatc
      781 gcgcacctgg tccaccagat tggtagcccg gagctgacga cccgacacat tgactgcaat
      841 ggtgcccagg gcatggccag cggctttcca ggcggccatc tgggtgcaca catggctcag
      901 tacccagggg tcgatctggt caatcaggcc cgtatcttcc gccagcggta tgaagcgcgc
      961 tggcgatatc cagcccagtt cggggtggtg ccagcgcacc agtgcctcgg ccccggatac
     1021 cttgccatcg cgcaggcgga tacggggctg gtaatgaacc gagagctggt tgcgtgcaat
     1081 ggcctggcgc aactcgtgtt ccaggaatgt gcgctcctgc aactcctggt tcatgctttc
     1141 ctcgaagaag accagacaac cccgcccggc attttttgca cggtacatgg cgatgtctgc
     1201 gcgcttgagg agttcttcgg cggtctggcc atcgtcggga tagaccgcca tgccgatact
     1261 gccaccaatg aaatgttcgg caccggcgat cacgaacggc tgcgccagta cctgcaggat
     1321 gccttcggcc atttttgcgg catgtcgggg gctctgcaac ccggtttgca gtacgacaaa
     1381 ctcgtctcca ccgaggcggg cgatggtgtc gctgtcgcgc acactcgact tcagtcgacc
     1441 agccacctgg cacagcaatt ggtcgccagc atcatggccc agggagtcgt tgatattctc
     1501 gaatcggtcc aggtcaatga acagcagggc cagctggctg ccgtcgcggc gggcgtgggc
     1561 cagctcctgc tccatgcgtt cgctcagaaa ctcccggttg ggcaaaccgg tcagtccatc
     1621 atggcgtgcc atgaatacca gttgcgcttc gcgggcgtgg gcggcaagtg ccacacccac
     1681 acgaccgccc aggtcgcgaa gctgctccag aatctgttcc ggcaaaccgc ttctgtcgtc
     1741 cgttcccaaa gccaccagtg cgacgacttc gtcgcgtgct actaccggca acagaaactg
     1801 gtgggctggc acacgttcac caaacaggga ggccgtgtgg ggggcgctgt gctggctgca
     1861 tccagccata ggcctgctgc gtggggtaac agggaagaaa gccgcaggtt ttcaagaggc
     1921 tggcgaaatt gctgcgactg gtgctcccct tttttgcgca ggtacaccgt tcccagattg
     1981 gcattcaatt cgtcaggtac cacaacgctc accagggcat gtgggaggat ttccagcatc
     2041 cgctgttgta cgcggtcaat gatctgtgca atgtcaatgt gggacaggat ttgctggtca
     2101 atccgggaca gcgcctgcat ggcgttgatg ttttgtccca gccggcttgc catggtgttc
     2161 agcgaatctg ccagttgccc gaactcgtcc tttcgggcga tgctgaccgg aacgtcaaat
     2221 tgttcacttt caatgcgacg tgtgccctcc atgaggcgtt ccagtggcac cagcgtgcgc
     2281 cggatttgcg agacgctcag caatgccacg aaaagcagtg tggccaccgc gatgcctatc
     2341 aatgtctggg ccatctgcgt cagtgactcg aatgccagcc caccgggacg catggcaatg
     2401 atggtccact cgggttgtgc aaacctggct tttaaaaaca atgcactgcg tgcggtcaac
     2461 cagccgtcga tctcgacggt ttttccactt tctccgcgca gactcgcgtt cactgctgtc
     2521 gcgcccaggt cagcgtcaga gcaggtcagc agggcggacc gctggtccag cacacagatg
     2581 ctggtctgaa atggaagtgt ttcggggtca tcccacaggt atttggggtc aagctccgcc
     2641 aggagttcgc ccctttcggg ctgtgttgta tcgatgcgca ctgcgagcca cagggttggg
     2701 atgcttccca cagcgtgtga tggcaacagc gccacctcac ctctggccag atggctttcc
     2761 aaagcgtcgg gaatctgtgg cgggggggat gcgaaggcag aaccccagcg catggcggcc
     2821 ttcccgtggg tgatgcgata gacgcgcagg aatgcgcttt cgaagttcgc cgggcggggg
     2881 ctggagccat tggtgaaaac gcgaatctgg gcggcatctg cgttcaggat gaaacttgct
     2941 cccagcaggt ggtcatacac cgttcgtgcg taggcgccag cggcctggct caactggcgt
     3001 tgcccggact cgctgaccac attgaacacg tgccggtagc tcagtacccc gagaatggct
     3061 acagggataa atgccgaaat aacgaagaga aaaaatacac gacgtgcaac tgcactgcgc
     3121 agaaaggttc cctggatgtg cagtttcaag gcgaaaagtc cccagccaag ccgacaaact
     3181 ggccatcacg accccgcacg atgtcatcga ggctgtcttt ttgcgtcagc tgcgttttgg
     3241 acaccccgtt ttttcccgtg ctgtagaggt cgtagtcaga gttgatgggg ttgaatttgt
     3301 ggtccttgcg ggccatgcca ttcccctgga cactggtcag gtcgacttag acgtaggggc
     3361 ggccccatgg atcaagcaaa ttggcattgc caacatcggc cagtgatgcc gggtaggtgc
     3421 caccactggc gaagtagtcg gtaatcagtg tctgcagaat cctgatatcg gtgatggctt
     3481 tggattggtc catccggtca cggtagttgt tgtatttggg aatcgcgatg gctgccagca
     3541 cgccgaccaa cgagacggcg atcaatacct caaccagtgt gaacccgaaa tgtcgacggt
     3601 ttcgcgagat atttaaaagc atgtttccag cgtttccccg tgtgttggct tggcacccgc
     3661 agtgtcaaac cgatcagcca tatccgatgg tccaaatgta aggatatgtt accgcatggg
     3721 gtatgggcct caaggcaggc ccgttgtgtg ctcgctggct agaaagtctc ccaatcgtcg
     3781 tcaccggatg ccggggcggg tttgggggac ggcttgggca gagctgcagg tgctgcggtg
     3841 gtgggccgcc ttttcggcgg tgcggctttt gcgctggctg ctgcgcgctg cgctggcgat
     3901 ccattggtgc ccagtgcccg gcgatgggtg tcctgagaag ggaggatttt gggtgcggca
     3961 ggccgcacgg aaaccttggg caggctgcct gcgccatcgg catcgttgag tttgaaagca
     4021 gccacaacct gcaccaggtc ttgcgcctgg gatttgaggc tgctggcggc cgcggccatt
     4081 tcttccacca gcgccgcgtt ctgctgtgtg gcatggtcca tttgggtaac agcgtcaccc
     4141 acctgggtta cgccctgtgc ctgttcgttg ctggcggcac tgatttcgcc cacgatatcc
     4201 gtgaccttct tgatgctgcc caccacttcg gccatcgtcg taccggcttc gtccaccagc
     4261 gtggtgccgc gttccacccg ctcgacactg gcgttgatca gggtcttgat ttctttggct
     4321 gcttccgcgc tgcggccagc gaggctgcgc acttcggagg ccaccacggc aaagccccga
     4381 ccctgttctc cggcacgggc agcttccacc gcggcattga gcgccagaat attggtctgg
     4441 aaggcgatgc cgtcgatcac actgatgatg tcggcaatct tgcgcgacga gtcgttgatg
     4501 cctttcatgg tttccaccac ctggctgacg acgtctccgc ccttgatggc caccgtggag
     4561 gcgttcaatg ccagttgatt ggcctgccgt gcgttctccg cgttttgttt cacggtggag
     4621 cccaggtggt ccatggaaga ggcggtctgc tgcaaggcac tggcctggtg ttccgtgcgg
     4681 gacgacaaat cgtggttgcc ctgcgcaatt tcggcgctcg cggtggccac gccctcggac
     4741 ccttgccgca cattgga
//
LOCUS       DAOSJM010000180         4751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15971_length_4751_cov_4.214225, whole genome shotgun sequence.
ACCESSION   DAOSJM010000180 DAOSJM010000000
VERSION     DAOSJM010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15971_length_4751_cov_4.214225"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..1181
                     /locus_tag="PKX82_07490"
     CDS             12..1181
                     /locus_tag="PKX82_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HOF52134.1"
                     /translation="MSSFEPTLQAAHARLAAVNPDAYARTRNALEGAVTRLSPYLTHG
                     FLSLREVYETVHARHPLNVQHKLVFELGWRAYWRHVWEHLGDGIHQSIHTGLLPDDAY
                     QSDMPADVLEARTGIAAIDQAVRELYATGYVHNHARMWLASYLVHLRKVHWHAGAQWM
                     LGHLLDGDVASNHLSWQWVAGTGSSKPYVFNADNVAQYAPAHWHSPGTVIDTSYEAMD
                     AMARSYTPIKPIPSRISGACATAVPSLVSAPSGSVWTAPDAALVDGRDVWLLHPWSVI
                     CPTSASHAGVLCVGVGLQAAHAGQPWSARRWDFVTQALQSQAQHLWWTDAPALADALT
                     GARSVRWHPNPHMDPFMALLKSDVSQSIAPPALFAPVDRYCASFSKWWSRTHLLD"
     gene            complement(1228..1743)
                     /locus_tag="PKX82_07495"
     CDS             complement(1228..1743)
                     /locus_tag="PKX82_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOF52135.1"
                     /translation="MQADTTDAWQALKTNGSIVLFRHAIAPGGGDPAGWVAGDCRTQR
                     NLSAEGQAQARRIGDTLRQRGVEVGAVWHSEWCRTRDTALLAFPAMSTAKLRPVPAFN
                     SFFNSPGSENMQTAEARRLLLGWRGPGVLVVVTHQVNITALTGLVPQSGEGVVLQTDE
                     TSLRVQGTLLP"
     gene            complement(1844..2545)
                     /locus_tag="PKX82_07500"
     CDS             complement(1844..2545)
                     /locus_tag="PKX82_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF52136.1"
                     /translation="MTELLTTDYRALVIGARGAIGHAFVDALQQDPHCSHVEMVSRSH
                     GSGFDLLDTASLAAQAAASAGAGPYRLIVDATGALTIDGVGPEKSLAAVQPDVLAKAF
                     AVNATGPLMVLRHFAPLLEPGPSIYAKLSARVGSIADNHKGGWYGYRASKAALNMLLQ
                     TAAIELQRKQPELRVVALQPGTVRSPLSQPFAAGVPKLLEPAESVAGMLATLKNLTAR
                     QGAHYVDYQGNEIPW"
     gene            complement(2575..2859)
                     /locus_tag="PKX82_07505"
     CDS             complement(2575..2859)
                     /locus_tag="PKX82_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HOF52137.1"
                     /translation="MMQAVVGCAAVGTALQVKAQAMLAETDAQAVALGYKADATKVDK
                     TKQPKYAAGQMCTNCALFQGAAKAAAGGCPLFAGKQVAGKGWCSAWAKKG"
     gene            3028..4038
                     /locus_tag="PKX82_07510"
     CDS             3028..4038
                     /locus_tag="PKX82_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOF52138.1"
                     /translation="MPNTHASQSRSTAGHRIGAVSALSGVPVPTLRGWEIRYGTFSPR
                     MTEGRQRLYNDDDVLRATLLRRLTEQGHSISGIANHDAAKLSALLHHQDASQRRNAPH
                     SAEREVPSVAVVGLALAARLASNKFRQIFSGNGLQVTDVFPDLDAALVQPLQTKPEFL
                     LVRTHSLHSVVQAQLHRLIDRSSVQQVIVLYHFGQEKVVESMKRAGMVVRREPVSDYE
                     LAELFRASLLMDAPQAMAHTGLGATIPPRKYDDATLARMAGMSSSMLCECPRHVAEII
                     AQLVSFEQYSQECLNKTSEDARLHAYLHSVSGSARALFEHALQMLAEHEGLALQEVEC
                     IG"
     gene            4026..4373
                     /locus_tag="PKX82_07515"
     CDS             4026..4373
                     /locus_tag="PKX82_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52139.1"
                     /translation="MHWLKKLEGTRRSASGLEWRIWRKLPLITLVGTLVPALLWVALD
                     WTLDPDSSAVHARWLLTASYVAWGAVIFHWTMVLTLAIGCAIVVVMKGPGYVADAYPL
                     SHSDSPSVQDRKE"
     gene            complement(4477..4731)
                     /locus_tag="PKX82_07520"
     CDS             complement(4477..4731)
                     /locus_tag="PKX82_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HOF52140.1"
                     /translation="MGVAHAADDKAPTKQQSKMKTCNVEAKDKKGDERKAFMKECLSA
                     KPAAAAEGNSQQSKMKTCNTEAKGKKGDERKKFMSECLKG"
BASE COUNT          869 a         1476 c         1444 g          962 t
ORIGIN      
        1 cgcgcaccgc catgtccagc tttgaaccca cgctgcaagc ggcccatgcg cgcctggcgg
       61 cggtcaatcc cgacgcctat gcccgcactc gcaacgcgct ggagggcgcc gtcacccggc
      121 tgtcgcccta cctgacccat ggctttctga gtctgcgcga ggtgtatgaa accgtgcacg
      181 cgcgccaccc actgaacgtc cagcacaagc tggtgttcga gctgggttgg cgtgcctact
      241 ggcggcatgt gtgggaacac ctgggcgatg gaattcacca atccattcac accggactgc
      301 tgcccgacga tgcctaccag tccgatatgc ccgcagatgt gctggaggcc cgcaccggca
      361 tcgcagcaat cgaccaggcg gtgcgcgagt tgtatgcaac cggctatgtc cacaaccacg
      421 cccgcatgtg gcttgccagc tatttggtcc acctgcgcaa ggttcattgg cacgcgggcg
      481 cgcagtggat gctgggccac ctgctcgacg gcgatgtagc cagtaaccac ctgagctggc
      541 agtgggtggc gggcacgggg agcagcaaac cgtatgtgtt caacgcagac aacgtggccc
      601 aatatgcgcc tgcccactgg catagccccg gtacggtcat agacaccagc tacgaagcaa
      661 tggatgcaat ggcgcgtagc tacacaccta taaagccgat acccagccgc atctctgggg
      721 cgtgtgcaac tgccgtgcca tccctggtgt cggcaccctc tggctccgtc tggactgcgc
      781 cagacgcggc gctggtcgat ggccgtgatg tgtggttgtt gcatccgtgg tcggtgatct
      841 gtccaacctc cgcatcgcac gccggggtgc tatgcgtagg tgtcggtttg caggctgcgc
      901 atgcgggcca accctggagt gcacgccgct gggactttgt gacgcaagca ctgcagagcc
      961 aggcccagca cctctggtgg acggatgccc ccgcgctggc agacgcattg acgggtgcgc
     1021 gcagcgttcg ctggcacccc aacccgcaca tggacccgtt catggccctc ctgaaaagtg
     1081 acgtgtcgca atccattgca ccgccggccc tgtttgcacc ggtggatcgt tactgcgcca
     1141 gcttttcgaa atggtggagc cgcacccatt tgctggacta agcggcgctg cgcgcctttg
     1201 tcaggcgtga tgtggccgct tagcgggtta cggcaacagg gtgccctgga cacgcaagga
     1261 tgtttcgtcg gtttgcaaca ccacgccctc gccggactgc ggcaccagtc ccgtcagcgc
     1321 cgtgatattg acctggtgcg tcacaaccac caaaacaccc ggaccacgcc agcccagcaa
     1381 aagtcggcgg gcttccgcgg tctgcatgtt ttcgctgcca gggctgttga agaacgagtt
     1441 gaaggccggc acaggccgca acttggcggt agacatcgca gggaacgcca acagggctgt
     1501 gtctcgggtc cggcaccatt cggaatgcca gactgcgccg acttccacac cgcgctgccg
     1561 cagcgtgtcg ccgatgcgcc gtgcctgcgc ctggccttcc gcactgaggt tgcgctgtgt
     1621 tcggcaatca cccgctaccc atcccgcggg gtcgccaccc ccgggtgcta tcgcatggcg
     1681 gaacaaaacg atggagccgt tggtcttgag ggcctgccat gcgtcggttg tgtcagcctg
     1741 cacggacaac gggctccacg ccagtacaga gacggaccac cccagcaggc aggcgcttcg
     1801 ggagcgtagt gcaatggaca tgggtgcgtc gggtggggct ggttcaccag gggatttcgt
     1861 tgccctggta atcaacatag tgcgcgccct ggcgtgccgt cagatttttc agggttgcca
     1921 gcatccccgc aaccgattcc gctggctcca ggagcttggg aacgcctgcc gcaaagggtt
     1981 gcgacaaagg cgatcgcacc gtacccggct gcaatgcgac cacacgcagt tcaggctgct
     2041 tgcgttgcaa ctcaatggca gcggtttgca gcagcatgtt caacgctgct ttcgaggcgc
     2101 ggtagccata ccagccaccc ttgtggttgt cggcaatgct gcctacacgc gcggacagct
     2161 tggcgtagat gctcggcccc ggttccagca agggcgcgaa gtggcgcagc accatcaggg
     2221 gtcctgtggc attgaccgca aatgccttgg ccagtacgtc cggttgcacc gccgccaacg
     2281 acttttccgg cccgacgcca tcgatggtga gcgcgccagt ggcatccacg atcagacgat
     2341 agggccctgc gccagcgctc gcggccgcct gtgctgccag gctggctgtg tccagcaggt
     2401 cgaatccgct gccgtgcgaa cgggacacca tttcaacgtg actgcaatgc ggatcctgct
     2461 gcaaggcgtc cacaaaggca tgcccgatcg cacccctcgc accgatgacc agggcccggt
     2521 agtctgttgt gagaagttcg gtcatggcag tacgtacaaa agactgcggc gcagtcagcc
     2581 cttcttggcc caggcgctgc accagccctt gccagccact tgcttgccgg caaacagcgg
     2641 gcaaccgccc gcggctgctt ttgccgcgcc ctggaacagg gcgcaattgg tgcacatctg
     2701 tcctgcggca tatttgggct gcttggtctt gtccactttg gtggcatcgg ctttgtagcc
     2761 caacgcgacg gcttgcgcat cggtttcagc cagcatcgcc tgtgctttga cttgcagggc
     2821 agtgcccacg gcagcgcagc ccacgacggc ctgcatcatg aatacgcggc ggttgtgggt
     2881 cagggaagtt gttgaggtct tcataggtac tccaggttgg ttgcatggaa atgcatgccg
     2941 aatgtagcat ttcgacaaga tatatctact caacttaata aatatttgct ataaactatt
     3001 caatacctat ccaatcacca gatgatcatg cccaacacac acgcaagtca atccaggtcc
     3061 actgccgggc atcgcattgg tgcggtatcg gcattgagtg gcgtgcccgt gccgaccttg
     3121 cgcggctggg aaatccgcta tggcaccttc agcccccgca tgaccgaagg ccgacagcgg
     3181 ctgtacaacg atgacgatgt tctgcgcgcc acactgctgc ggcgcctcac ggagcagggg
     3241 cactccatca gcggtattgc caatcacgat gcagccaagc tcagcgcctt gttgcaccac
     3301 caggacgcat cccagcggcg caatgcgccc cacagcgcag aacgcgaggt cccgagcgtg
     3361 gcggtggtcg ggcttgcatt ggcggcgagg ctggcctcaa ataaattcag gcaaattttt
     3421 tccggcaacg gactgcaagt gaccgacgtt tttcccgacc tggatgcggc gctggtgcag
     3481 ccgttgcaga ccaagccaga gtttctgttg gtgcgcaccc attccttgca ctccgtggtg
     3541 caggcgcagt tgcaccgttt gatagaccgt agctctgtcc agcaagtcat tgtcctgtac
     3601 cacttcgggc aggaaaaggt cgtggaatcc atgaagcgcg ccggcatggt ggtgcgtcgc
     3661 gagccagtgt ccgactacga acttgccgaa ttgtttcgcg cgtccctctt gatggacgcg
     3721 ccccaggcga tggcgcacac cggtcttggc gccacgattc cgcctcgcaa atacgacgat
     3781 gcgacgcttg cgcgcatggc cggaatgtca agcagtatgt tgtgcgaatg tccccgccac
     3841 gtagcggaaa tcattgcgca gttggtcagc ttcgagcagt acagccagga atgcctgaac
     3901 aagacctccg aagatgcgcg cctgcatgcc tacctgcatt cagtgtccgg ttcggcacgc
     3961 gccctgtttg agcacgcact tcaaatgctg gccgagcacg aagggctggc actgcaggag
     4021 gtcgaatgca ttggctgaaa aaactggaag gcacccggcg ctctgcgagc ggcctggaat
     4081 ggcgtatctg gcgcaagttg ccgttgatca ccctggtagg aaccttggtg ccagcgctgt
     4141 tgtgggtcgc gctggattgg actctggacc cggactccag tgcggtgcat gcgcgctggc
     4201 tgctgacggc cagttatgtg gcctggggtg cggtgatctt ccactggacc atggtgctga
     4261 ccctggcgat tggctgcgct atcgtggtag tgatgaaggg tccgggttat gtggccgatg
     4321 cctacccgct atcccacagc gacagcccct cggtgcagga ccgcaaagag taatttcact
     4381 gcccatcgca gcatgcggac ctccgtggct tggggtgctg cgcgaacata aaaaaaccgg
     4441 cccgcaaagc ggccggtttt ttggagactg aaagaatcaa cctttcaggc actcgctcat
     4501 gaacttcttg cgctcgtcgc cttttttgcc cttggcttcg gtgttgcagg ttttcatctt
     4561 gctttgctgg gaattgcctt ctgcggcagc agccggcttg gcgctcaggc attccttcat
     4621 gaaggccttg cgctcatcac ctttcttgtc ctttgcctcg acattgcagg tcttcatctt
     4681 gctctgttgc ttggtgggtg ctttgtcgtc ggcagcgtgg gccacgccca ttgccagtgt
     4741 caaacccaag g
//
LOCUS       DAOSJM010000181         4749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_15981_length_4749_cov_3.554751, whole genome shotgun sequence.
ACCESSION   DAOSJM010000181 DAOSJM010000000
VERSION     DAOSJM010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15981_length_4749_cov_3.554751"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PKX82_07525"
     CDS             <1..185
                     /locus_tag="PKX82_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOF52141.1"
                     /translation="HVSKLIWNITETIEQLSAAWTLQPGDLIFTGTPEGVAAVVRGDV
                     LAGSVDGLQAIRVELI"
     gene            205..750
                     /locus_tag="PKX82_07530"
     CDS             205..750
                     /locus_tag="PKX82_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOF52142.1"
                     /translation="MLRHPIKHCRQCGTAVTYRVPDDGDTKERAVCPSCSTIHYENPL
                     NVVGTVPHWGDKVLLCKRNIEPRWGKWTLPAGFMELNETTAEGAARETDEEAGAQFEM
                     QGLFTLLNVARVGQVHLFYRARLTSDQFNPGHETIEARLFAEDEIPWDEIAFRTVKET
                     LERYFADRLTGDFGVHTVDIV"
     gene            756..1898
                     /locus_tag="PKX82_07535"
     CDS             756..1898
                     /locus_tag="PKX82_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOF52143.1"
                     /translation="MLPCYVVLDLETTGGNASQDRITEIAAVRMEDGQEVARWSTLVH
                     PGVPIPPFIQSLTGITDAMVADAPDFAEVAPELIRILDGAVLVAHNVRFDHGFVQNEL
                     ARLDMALKTKTLCTVRLSRKLYPQHKGHGLDALLLRHGLHTETRHRAMGDVEVVLAWL
                     RVVTAELGLDQVRHEAQVLLQGGTSLPPHLETSISDIPETVGVYLFYGEGTRPLYVGK
                     GVHLRTRVMSHFQSANKVAREMRLLQGVRRVEWRETAGELGAQLLKSRLVKELQPVYN
                     RKLRRHEMPALPSWPYSGPVAIREHDATSGRTDIHVFDRWCHLATVQDPAALDEALQA
                     RQSLVFDLDTYRILLKRLTQPAGRDPSVFSLNRTELTPDAWVDQPN"
     gene            2040..3758
                     /locus_tag="PKX82_07540"
     CDS             2040..3758
                     /locus_tag="PKX82_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52144.1"
                     /translation="MTLGRARGAAWIGQPLSLSVPVQVEPGQTAATLCAEADVFHAEN
                     KLESSRVTVAVEPTAQPETFNLRITSSALIDEPVVTVYLRAGCAQKTTRRYVLLADYP
                     SETLAPTAPTPVEVVPAKPVAVPAVEPATPAPASSNTTAAPAKAPAGTVTASKTGTTP
                     QAAKPAPAKATPAAPKPAISTTAKPASAPAAKTAESARPRLKLDPLENLAERIKTLEA
                     TTSSVPLEDMVRDSQRIQQLQNDVKTLLDQAAKNEASLLLMRERLERAEAERVSSLVI
                     WVLGALVLICLAAIAALWNRRTAAAPSAWQQSIQEAPYTVAQSAQETTQFKVEQPAAV
                     VTPVAAKDETAPNSTILMPVAPSTMDVDVNLMDMDSDFSRLIATPSAAHPPTQLMEYP
                     NSRFQDTRTLHRDFNSDSLTDVRQQAEFFEKLGKVDQAIDVLEQRIRLNSKEAPLLYL
                     DLLGIAHGHDLKTDFRQFRDEFEQLFNVSAPEFALFRDEGRSLESYASLMQHICKLWP
                     TAGVLDVIETCIVRDPWEKNADPFDIGAFRDLILLHGVASRRLRDDSAARGLGDADSQ
                     HVDIDM"
     gene            complement(4195..>4749)
                     /locus_tag="PKX82_07545"
     CDS             complement(4195..>4749)
                     /locus_tag="PKX82_07545"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOF52145.1"
                     /translation="LGKEQEDWLNSNLAKQTPGEGQVWNFLVQTTMFSPRRIPWNGDY
                     KLWNDGWDGYPAARDRLVAALQQYKVSGPVFIGGDLHENWACNVPGRDLDSNVRTIAS
                     EFVGTGITQSSYYPKGVDFIKPANKHAVYADAGRCGYGTVDISKARIEVRYRVVDNIA
                     KSDPAISTAATFVVESGNPEIHPV"
BASE COUNT         1041 a         1455 c         1330 g          923 t
ORIGIN      
        1 gccatgtgtc caagctgatc tggaacataa ccgaaaccat cgaacagctc tctgccgcct
       61 ggacactgca acccggcgac ctgatcttca ccggcacgcc cgagggtgta gccgccgtcg
      121 tgcgcggtga tgtgctggcg ggcagtgtgg atggtctgca agccattcgt gtcgagctga
      181 tctaaccgca caccaacttc actcatgctg cgtcatccca tcaaacattg ccgtcagtgc
      241 ggcacggccg taacctaccg tgtgcccgac gacggagaca ccaaggaacg cgccgtgtgt
      301 ccatcgtgct ccaccatcca ctacgagaac ccgctcaacg tggtgggcac cgtgccgcat
      361 tggggcgaca aggtgctgct gtgcaagcgc aacatcgaac cccgctgggg caagtggacg
      421 ctgcccgcgg ggttcatgga actgaacgaa accaccgccg agggcgcggc ccgcgagacc
      481 gacgaggagg ctggcgccca gtttgaaatg caggggctct tcaccctgct caatgtggcg
      541 cgcgtcggac aggtgcacct gttttaccgt gcgcgactga ccagtgacca gttcaacccg
      601 ggccacgaaa ccatcgaagc ccggcttttt gcagaagacg aaatcccctg ggatgaaatt
      661 gcgtttcgca ccgtcaagga gacgctggag cgttactttg cggaccgcct cacgggtgat
      721 tttggcgtgc acaccgtaga catcgtctaa cacgcatgct cccctgctat gtcgtgctgg
      781 acctggagac cacgggtggc aatgcatccc aggaccgcat caccgaaatc gccgctgtgc
      841 gcatggagga tggtcaggag gttgcccgct ggagcacgct ggtccatcct ggcgtaccta
      901 ttccgccttt catccagagc ctgaccggca tcaccgacgc catggtcgct gacgcgccag
      961 acttcgcgga agtggcaccc gagctgatcc ggatactcga tggcgccgtg ctggtggccc
     1021 acaacgtgcg ctttgaccac ggcttcgtgc agaacgaact ggcccggctg gacatggcat
     1081 tgaagaccaa aacactgtgc accgtccgcc tgtcgcgcaa gctatatccg caacacaagg
     1141 gccacggact ggacgcactg ctgcttcgcc atggactgca caccgaaacc cggcaccggg
     1201 ccatgggcga tgtggaggtc gtactggcct ggctgcgggt ggtgaccgca gagctgggcc
     1261 tggatcaggt gcgacacgag gcacaggtgc tgctgcaagg aggcacgagc ctgccaccgc
     1321 atctggaaac ttcgatctct gacataccgg agaccgtcgg tgtctacctg ttttacggtg
     1381 aaggcacacg gccgctgtac gtcggtaaag gcgtccatct gcgcacgcgg gtcatgtcgc
     1441 acttccagtc ggcgaacaaa gtcgccaggg aaatgcgcct cctgcaggga gttcgccggg
     1501 tggagtggcg tgagacggcg ggcgaactgg gtgcccaact gctgaaatcc cgtctggtca
     1561 aggagctgca acccgtctac aaccgcaagt tgcgccgcca cgaaatgcca gcgctgccat
     1621 cctggccata ctctggtcct gtggccatcc gtgaacacga tgcgacatcc ggacgcaccg
     1681 atattcatgt gtttgaccgc tggtgccatc tggccaccgt gcaggaccct gcagcgctgg
     1741 acgaagcact gcaagcgcgc caatcgctgg tgttcgatct ggacacctac cggattctgc
     1801 tcaaacgcct gacgcagcct gccgggcgtg atccatcggt tttcagtctg aaccgcacgg
     1861 aattgacccc agacgcctgg gtagatcagc caaactgatc tttgtcaatc aggggcaagg
     1921 atgttcccga aatccggatc gccgtgcgag aatgtaaaaa agtgttagag gcggttatga
     1981 gaatcaaggt tgtcgtactg gggggggtct tggcttgcgc tgcgttggca agcatggcca
     2041 tgacgttggg cagggcacgc ggtgccgctt ggattgggca accgctctct ttatcggtac
     2101 cggttcaggt agaacccggg cagactgcgg ctacgctgtg cgccgaagcc gatgtattcc
     2161 atgccgagaa caagctcgaa tccagccggg tgacggttgc cgtggaacct actgcgcagc
     2221 cggaaacatt caatctgcgc atcacgtcat ccgccctcat cgacgagccg gtcgtgacgg
     2281 tctacctccg ggcaggatgt gcccaaaaga cgacacgccg ctatgttctt ctggccgact
     2341 atccgtcaga aaccctggct ccgacagcac ctactccggt ggaagtggtg ccggcaaaac
     2401 cggtcgcggt tccagcagtg gaacctgcca cgcccgctcc tgccagcagc aacaccacgg
     2461 cggcacctgc aaaagcaccc gcgggcacag tgacagccag caagacgggt actacaccgc
     2521 aagcggccaa accagctcca gccaaagcca cacccgcagc ccctaaaccg gctatcagca
     2581 caacagcaaa gccagcatcc gcaccagcag caaaaactgc ggaatctgcc cgcccgcggc
     2641 tcaagctgga cccacttgaa aacctcgccg aacgcatcaa gacactggaa gccacaacat
     2701 catcggtccc tctggaggac atggttcgcg actcccaacg tattcagcaa ctgcaaaacg
     2761 atgtcaagac cctgctggat caggctgcca agaacgaagc cagtctgcta ctcatgcgtg
     2821 aacgactgga gcgggccgaa gccgaacgtg tgtcatccct ggtgatctgg gtactgggcg
     2881 cgctggtcct tatttgcctt gcagccatcg ctgcgctctg gaaccgccgc actgccgcag
     2941 cacccagcgc ctggcagcaa agcattcagg aagcccctta caccgttgcc cagtcggccc
     3001 aggaaaccac gcaattcaaa gtcgagcaac ccgcagccgt ggtcacccct gttgccgcca
     3061 aggacgaaac cgctcccaac tccacgattc tgatgcctgt cgcacccagc acgatggatg
     3121 tggatgtcaa tctgatggat atggactccg acttcagtcg gctcattgcc accccgtcag
     3181 ccgcacatcc cccaacgcaa ttgatggagt acccaaactc ccgatttcag gacacccgga
     3241 cgctccaccg cgatttcaat tcggacagtt tgaccgacgt acggcagcag gcagagttct
     3301 tcgagaaact cggtaaggta gaccaggcca tagacgtgct ggaacagcgc atccgcctca
     3361 acagcaaaga agcaccgctg ctgtacctcg acctgctggg cattgcacat ggtcacgatc
     3421 tcaaaacaga cttccgtcag ttccgggatg agtttgagca gctgttcaat gtcagcgccc
     3481 cggagtttgc cttgttccgt gacgaagggc gtagcctgga atcctatgca tccctgatgc
     3541 agcacatctg caaactctgg cccaccgccg gcgtgctcga tgtgattgag acctgcatag
     3601 tgcgcgatcc gtgggagaaa aatgcggacc catttgacat cggcgccttc cgcgacctga
     3661 tcctgctgca cggcgtggca tcaaggcgac tgcgagacga tagcgcagcc cggggactgg
     3721 gtgacgctga ctcccagcat gtcgatatcg acatgtagga aaagccccca gacaatcgat
     3781 ttgatcttta cgatttatgt gagttggcta tgcacgcagt cactctggag gagtgctttg
     3841 agcataggga gcgatttctc cgccagctcc ccacatggat cgacaaggat gcactgctgg
     3901 ggtatgcgct gaaaactgag ttcaccatcc gcagaagcat tcaccaccat gtcctgatcc
     3961 tgttggatgg ctcaaagctt tgcaacgcag aggtaatctg ccacatgctg ggctatcagg
     4021 cgggcaggag gcggttttgc attgcaagtc agtcagcatc ttcaatcctc tatccccaaa
     4081 actcgcgaaa gggggaacag caataaaaaa agggggaaca tgtcggttgt tcccccgcat
     4141 gttcccccac cttgggttga cgtactttgg tgttgtgtca tcaccagcgg ttacttacac
     4201 cggatggatt tcagggttac cggactcgac tacaaatgtg gctgcggttg atatcgcagg
     4261 atcagatttg gcgatgttgt ctacaacccg gtatctaact tcgatccgtg cttttgaaat
     4321 atcaacggtt ccgtacccac atctgcctgc atccgcgtaa acggcatgct tgttagcagg
     4381 tttgatgaaa tcaacaccct ttgggtaata ggaactctga gtgatacctg ttccaacgaa
     4441 ttcactggct atggtccgta cattgctatc caagtcgcgt cccggcacgt tgcaggccca
     4501 gttctcatgc agatccccac caataaatac gggacctgaa accttgtatt gctgcaatgc
     4561 cgcgacgagg cgatctcgtg cagcggggta accatcccag ccatcgttcc acaatttgta
     4621 gtccccgttc cagggaatgc ggcgtggcga aaacatggtc gtttgcacaa ggaaattcca
     4681 gacctgtcct tcgccagggg tttgcttcgc caaattggaa ttcagccagt cctcctgctc
     4741 cttcccgag
//
LOCUS       DAOSJM010000182         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16225_length_4694_cov_3.439534, whole genome shotgun sequence.
ACCESSION   DAOSJM010000182 DAOSJM010000000
VERSION     DAOSJM010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16225_length_4694_cov_3.439534"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PKX82_07550"
     CDS             complement(<1..531)
                     /locus_tag="PKX82_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOF52146.1"
                     /translation="MHDLAFVFAGFFVGLVVGLTGVGGGSLMTPILIFFFGVKPYMAV
                     GTDLLFAAFTKMGGTVKLARSRHIDWPVVLNLSAGSIPAALITLYILHTLGATSPEVQ
                     RLMTTTLGFALLMTAAATLYKALRGKAAPISVAKGQEAVAARPRHWSLPLLFGALIGT
                     LVTLTSVGAGAIGVTVL"
     gene            complement(637..1398)
                     /locus_tag="PKX82_07555"
     CDS             complement(637..1398)
                     /locus_tag="PKX82_07555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF52147.1"
                     /translation="MNTSSTVPPLVTDELSVLSSMVPLADQHIIELGCGSARLARQLL
                     ERYPGSQVTGLETDTRQHAKNLAAPQERLTFILAGAQGIPLADAAFDLALMLKSLHHV
                     PIPLMRQALAEVARVLRNGGHLYVSEPVYAGPLNDIVRVFNDEGPVRLAAQHAVDDAV
                     ASPAWVQVAERRFDMPVHFKDFADFERRMMYPTFADHHVDAHITAEARKRFEPHCTDD
                     GARFTRPMHVRLLQKQPHALRDSEKGVKLTHTRSR"
     gene            1474..2670
                     /locus_tag="PKX82_07560"
     CDS             1474..2670
                     /locus_tag="PKX82_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF52148.1"
                     /translation="MKKLLVAWLGTVALGAMAQGTPIKIGEINSYSSIPQFTTPYRQG
                     WQLAVEEINASGGLLGRKVEVIARDDAGKPEEALRHAVELTSSEKVDVLAGGFLSNVG
                     LALADHAQKNKRLFVASEPLTDALVWDKGNRYTFRLRPSTYMQAAMLVEEAAKLPAKR
                     WATIAPNYEYGQSAVASFKELLKTKRPDVEFVAEQWPVLGKLDAGTTLQAVMQAKPDA
                     IFNVTFGADLAKLAREGNQRNVFPKTPVVSLLSGEPEYLDVLKDETPRGWIVTGYPWD
                     QIDSREHASFATNYYKKFGENPKVGSVVGYATMQAIFAAITKAKSTDNEKLVAAMRGL
                     KFSTPFGPAEFRALDQQSTMGAFVGKLDQRGGKGTMVQWRYADGKNYQPTDAYVRNRR
                     PAAAMK"
     gene            2682..3419
                     /locus_tag="PKX82_07565"
     CDS             2682..3419
                     /locus_tag="PKX82_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52149.1"
                     /translation="MATPTWVKVVGFDDAERHSLNTLFRLSERMSPSYVLWAPECPSQ
                     PHVALIDLDSYEGGLEIASPRFNPNLKLICVGGKPPAHAWRTFTRPVDWSAMLRVLDG
                     LFAAKSDVDIDVETGEGAERTVPPGVKLSLLVGLARQDRLYLRARLALAGMTDVEDVD
                     TAADAAAKVSKRQYDLVVISLELIDADPWALVQTLKNMPTPVRSIIIATDAPSWRAME
                     MAEHLGCTGMLEIPFDPQQVVELLQKV"
     gene            complement(3407..>4694)
                     /locus_tag="PKX82_07570"
     CDS             complement(3407..>4694)
                     /locus_tag="PKX82_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52150.1"
                     /translation="LCRRGTLLRASTPGTLPAALLEDAGFCLNTPAEPQAPTEYRYDP
                     RWNLMHSRKSHGSATVSRCAVIGAGMAGASVAHVLALRGWAVSVLDACAEPAGGASGL
                     PVGLVVPHVSVDDSPRSRMSRNGTRLMLLHAQRLLVEGLDWGHTGVMERRGAPEGDLW
                     HPMAGWIKPARLVQEWLRHPAIAFVGNAPVRQLTHNEGVWTLHGDQGQLLAQAEHVVF
                     ANAYGCRELLHSMASTVELEGDVLAKSDTWQELHGLLSYGMCPADPQRAANGMWPTGP
                     VNGDGSFVPNIHTLQGLLWFAGSTFETQTAQVADIAEQHRANHARLASLLPEVGAALA
                     PDFAAGTVQAWSATRCATHDRLPLVGPLQTNATPSLWINAGMGSRGLSFSALCAELLA
                     SQMHGEPLPVEAQLARGLDSMRARRQRRTTTETQTF"
BASE COUNT          886 a         1443 c         1523 g          842 t
ORIGIN      
        1 cagcacggtg acaccaatgg cgcctgcgcc caccgaagtc agtgtgacca gcgtgccgat
       61 cagtgcgcca aacagcaggg gcaggctcca gtggcgcggg cgagcggcca cggcttcctg
      121 gcctttggcc acgctgatgg gcgctgcctt gccgcgcagt gccttgtaga gcgtggccgc
      181 tgcagtcatc agcagggcaa aacccagcgt agtggtcatc agccgctgca cttccgggct
      241 ggtggcgccc agggtatgca agatatagag cgtgatgagc gccgcgggaa tgctgccggc
      301 cgacaggttg aggaccaccg gccagtcgat atgcctggag cgcgccagct tgacggtgcc
      361 gcccattttg gtgaaggccg caaacagcag gtcggtgccg accgccatat agggcttcac
      421 gccaaagaag aagatcagga tcggcgtcat gagcgaaccg ccgcccacac cggtcaggcc
      481 gacgaccaga ccgacaaaga accccgcaaa gacaaacgcc aaatcatgca tggccgcgac
      541 tgtaggcggc atggcatata ttgaaaacta catttttgct atttccatat gtcgttcgga
      601 tatatgtagc gataaagcaa gcgcacccca cccagctcat ctggaccggg tgtgggtcag
      661 tttgacgcct ttttcactgt cacgcaaagc gtggggctgc ttctgcagca ggcgtacgtg
      721 catgggtcgg gtgaaccggg cgccgtcgtc cgtgcaatgg ggctcgaacc gcttgcgtgc
      781 ctcggccgtg atgtgcgcgt ccacatggtg gtcggcaaac gtggggtaca tcatgcggcg
      841 ttcaaagtcg gcaaagtcct tgaaatgcac tggcatgtca aagcgccgct ccgcaacctg
      901 tacccaggcg ggggatgcca ccgcgtcgtc cacggcatgc tgtgcggcca gacgcaccgg
      961 cccttcgtcg ttgaatacac ggacgatgtc gttcagcggg cctgcataga ccggctcgga
     1021 cacatacagg tggcccccat tgcgcaacac ccgcgccact tctgccagtg cctgccgcat
     1081 cagggggatg ggtacatggt gcagcgactt gagcatcagt gccaggtcaa aggcagcgtc
     1141 cgctaggggg atgccctgcg ccccggccag tatgaaagtc agccgctcct gcggcgccgc
     1201 caggttcttg gcgtgctgtc gcgtatcggt ctccagcccg gtaacctggc tgcccgggta
     1261 gcgctccagg agttgccggg ccagcctggc agagccacac cccagttcga tgatgtgctg
     1321 atcggccagc ggaaccatgc tggaaagtac cgatagttcg tctgttacca gggggggaac
     1381 ggtggaggag gtattcatgg tggcggtgtg caaggtctcg gcgatggttg acacagcata
     1441 aacgctgcta gagttcaagc ctgcggagaa attatgaaga aattgcttgt tgcatggttg
     1501 gggacggtgg cgttgggcgc gatggcccag ggcacgccta tcaagattgg agagatcaat
     1561 agctactcca gcatcccgca attcaccacg ccctatcggc aggggtggca actggccgta
     1621 gaggagatca atgccagtgg cggcctgctg gggcgcaagg tcgaagtcat cgcacgtgac
     1681 gacgctggca agcccgaaga ggctttgcgc cacgctgtcg agctgacctc cagcgagaaa
     1741 gtggacgtgc tggctggcgg attcctctcc aacgtcgggc tggcgctggc cgaccatgcc
     1801 cagaagaaca agcgcctgtt tgtggccagt gagccactga ccgatgctct ggtgtgggac
     1861 aagggcaacc gttatacctt ccggctgcgc cccagcacct atatgcaggc ggccatgctg
     1921 gtggaagaag ctgccaaact gccggccaag cgctgggcca cgattgcgcc caattacgag
     1981 tacggacaga gtgcagtggc cagcttcaag gagttgctca agaccaagcg tccggacgtg
     2041 gagtttgttg ccgagcagtg gccggtgctg ggcaaacttg atgctggcac caccttgcag
     2101 gcggtgatgc aggccaaacc ggatgccatt ttcaacgtga cattcggggc cgatctggcc
     2161 aagctggcgc gcgaaggcaa ccagcgcaat gtattcccca aaacgccggt ggtgtccctg
     2221 ctgtcgggtg agccggagta cctggatgtg ctcaaggacg agacgcccag gggctggatc
     2281 gtgaccggct acccctggga ccagattgac agccgcgaac atgcgtcgtt cgcgaccaat
     2341 tactacaaga aatttggcga aaaccccaag gtgggttcgg tggtgggcta cgccaccatg
     2401 caggccatct ttgcagccat taccaaggcg aaaagtactg acaacgagaa actggtggcc
     2461 gccatgcgcg ggctcaagtt cagtaccccc tttggccccg ccgagttccg tgcgctggac
     2521 cagcaatcga ccatgggcgc atttgtgggc aagctggacc agcgcggtgg caagggcacc
     2581 atggtgcagt ggcgctatgc cgatggcaaa aattaccagc ccacggacgc ctacgtccgc
     2641 aaccgccgcc ccgctgcggc catgaagtaa ggcgcagcaa catggcaacc cctacctggg
     2701 tcaaggttgt cggctttgat gacgcggagc ggcattcact caatacgctg tttcgactgt
     2761 ccgaacggat gtctccgtcc tatgtgctgt gggcaccgga gtgcccttcg cagccccatg
     2821 tggcgctgat tgatctggac agttacgaag gcgggctgga aatcgcatcc ccccgcttca
     2881 accccaatct caagctgatc tgcgtcggcg gcaagccacc agcccacgcc tggcgcacgt
     2941 ttacccgtcc agtggactgg agcgccatgc tccgggttct ggatggtctg tttgcggcca
     3001 agtccgatgt ggacatcgat gtcgagaccg gcgaaggcgc cgagcgaacc gtgccccctg
     3061 gggtcaagct cagcctgctg gtggggttgg cgcggcagga tcgcctgtac ctgcgcgcgc
     3121 gcctggcgct ggcgggcatg accgatgttg aggatgtgga tacggccgct gacgctgccg
     3181 cgaaggtgtc caagcggcaa tacgatctgg tggtgatcag cctggagttg atcgatgccg
     3241 acccctgggc actggtgcag acactcaaaa acatgcccac gccggtgcgc tccatcatca
     3301 tcgccaccga tgcaccttcg tggcgcgcca tggaaatggc cgagcacctg gggtgcaccg
     3361 gcatgctgga aattccgttc gacccgcagc aggtcgtgga actgcttcag aaagtctgag
     3421 tctccgtcgt cgtgcggcgc tgccggcgcg ctcgcatgga gtccaggccg cgcgccagtt
     3481 gggcttccac cggcaggggt tccccgtgca tctgcgaagc cagcagttcc gcgcagagcg
     3541 ccgaaaagct cagtccgcgt gagcccatcc ctgcgttgat ccacaagctt ggtgttgcgt
     3601 tggtctgcag agggcccacc agcggcaggc ggtcgtgcgt ggcgcaccgt gtggcagacc
     3661 acgcctgcac ggtccccgcg gcaaaatccg gtgccagcgc ggcaccgacc tcgggcagca
     3721 gcgacgccag ccgcgcatgg ttggcgcggt gttgttctgc aatatcggcc acctgggccg
     3781 tctgtgtttc aaaggtcgac ccggcaaacc acagcagacc ctgcagtgtg tgtatgttgg
     3841 gcacaaaact gccgtcgccg ttgaccggac ccgtgggcca catgccgttt gccgcccgct
     3901 gtggatcggc ggggcacatg ccgtaactca gcaagccgtg cagctcctgc catgtgtcgc
     3961 tttttgcgag gacatcacct tccagctcaa cagtggaggc catgctgtgc agcagttccc
     4021 ggcagccata ggcattggcg aagaccacgt gttctgcttg cgccagcagt tggccttggt
     4081 cgccgtgcag cgtccagaca ccttcgttat gggtcagctg gcgtactggc gcgtttccca
     4141 cgaacgcgat ggccgggtgc cgcaaccatt cctgcaccag tcgtgcgggc ttgatccagc
     4201 cggccatcgg atgccagagg tcgccttccg gtgcgcctcg gcgctccatc acgccggtgt
     4261 gtccccagtc cagtccttcg accagcagac gctgcgcatg cagcagcatc aggcgggtgc
     4321 cattgcggga catgcgtgag cgcggactgt cgtcgacgga tacatggggc accacgaggc
     4381 cgacgggcag gcccgacgcg cctccggcag gctcagcgca ggcatccagc acgctgaccg
     4441 cccagccgcg cagagccagc acatgggcca cgctggctcc ggccatacca gcgccaatga
     4501 ctgcgcaccg gctgacggtc gcgctgccat ggctcttgcg ggaatgcatc aggttccagc
     4561 gcgggtcgta acggtattcg gtgggagctt gcggttccgc tggcgtgttc agacagaagc
     4621 cggcgtcttc cagcagggcc gcgggcaagg tacccggggt gctggcgcgc agcagcgtgc
     4681 ctctgcggca cagt
//
LOCUS       DAOSJM010000183         4684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16260_length_4684_cov_4.954850, whole genome shotgun sequence.
ACCESSION   DAOSJM010000183 DAOSJM010000000
VERSION     DAOSJM010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16260_length_4684_cov_4.954850"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PKX82_07575"
     CDS             complement(<1..254)
                     /locus_tag="PKX82_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HOF52151.1"
                     /translation="MPETPVLASRLWKGGWYQFARPRPSPNFGPRPALAHIDLIVLHS
                     ISLPPGEYGGSGVLELFTNQLNWDSHPYFKTIEGLQVSAH"
     gene            complement(247..1725)
                     /locus_tag="PKX82_07580"
     CDS             complement(247..1725)
                     /locus_tag="PKX82_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOF52152.1"
                     /translation="MATNTLLSNIHILVIDDEPDLRTLYELTLLREGYQVTTAGTVEQ
                     AREALHAGRFDLVITDMCLPDGMGTELLKDLRNQQRSERCIVITAYGSAENAVESLKA
                     GAFDYLTKPVDLKQFRSVVAAAIHGTNTADRPKEVSSASDTRATRRTGRTTGGEAALS
                     LLAGDSPAMRFVKDRIVKVARSMAPVLVCGESGTGKELVANALHANSHRSEGPWVAVN
                     CGAIPENLLEAEFFGSKKGSYTGASQDREGFFKAANGGTLFLDEIGDLPIAMQAKLLR
                     AIQERCIRPIGGSQEEPVDVRIVSATHKDLPAEVAAGRFRQDLFYRLNVIDIHIPPLR
                     DRIEDLPALCQSLIQRIAAESGQSPPKLAEIALGQLSQLPLNGNVRELENLLHRAMAL
                     TDGDELQLDDCMSIATRPAPIEATTTPAPLSAPRAEDIPSDLQTHLDQQERDILVRTL
                     KETGFNRTLAATRLGLSLRQIRYRIARLKIDTPQSAEDEADA"
     gene            complement(1704..3449)
                     /locus_tag="PKX82_07585"
     CDS             complement(1704..3449)
                     /locus_tag="PKX82_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF52153.1"
                     /translation="MDDDATQHSWFGPPDIAAVLESTEESREYVRQWQGFMTARLALS
                     VVMVALQGSLYFTGFAQSRLLVAICVAYFVATLASRLLEKPHALGTSFNKSWLSLVGV
                     DVVAFSALQIVQGNNINYTPLFALPVLLSAVLGPLRLALGTAAGVTVLLLVNTTWTHL
                     TSLADATAYFVQSALSGVGYFVIALLANQLSTRLANAGQRARLNQAAANIQRHVNELV
                     IESLQDGVVIVDASGRVLAANPAARRLLGVAHAQPHLLDLKAHAGWAPLWNLTRVCIG
                     TGEGLEEDVTLRQGALGVQRIKVRTRLAAPQGGGADSLCVLFLQDLRALEARMRTEKL
                     ASMGRMSTAVAHEIRNPLAAISQANALLEEDLGDPRQIRLTDMIGQNAKRLGKIVDDI
                     LHAAQVGPHAKETSNLSLALNEHTHRICHEWAQQNGEAPRLVTLLSGPALLVQFEIEH
                     LRRVLVNLLDNARRYATSQAEAIQVSSRVLDERHAMLSVWSDGQPMEQSVERHLFEPF
                     FSSESRSSGLGLYICRELCERHGATIVYQRTSRIARGQSTDGNEFVITLAQASTPASA
                     NEATAKTSPWQPTLY"
     gene            complement(3677..4474)
                     /gene="ccsA"
                     /locus_tag="PKX82_07590"
     CDS             complement(3677..4474)
                     /gene="ccsA"
                     /locus_tag="PKX82_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOF52154.1"
                     /translation="MILASASPVQLILSVLAASAYAWPALRATRMSLRTSHLWVAVAW
                     LLHGLVLAMGLMGDPAHFGFAPALSVTAWLVAAVYAVESLVYPQLSTRWTLCALGAAA
                     VLLAYAFPGNTLPATASVWLPVHLAFAIACYGLFGMAVVHALFMNHAEKRIRMAEDSH
                     SGLPLLTLERLTYRFVAAGFALLSLTLLVGMLFGEVVYGHAWRWDHKTVFSLLSWVTF
                     AVLLAGRWLAGWRGKRAAMVLYIGTAFLLLAYVGSRFVMEIFLGRTA"
     gene            4552..>4684
                     /locus_tag="PKX82_07595"
     CDS             4552..>4684
                     /locus_tag="PKX82_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOF52155.1"
                     /translation="MASALSDKLSRLVKDLRGQGRITESNVTDMLREVRMALLEADVA
                     "
BASE COUNT          986 a         1370 c         1370 g          958 t
ORIGIN      
        1 aagtgcgccg acacttgcaa gccttcaatg gttttgaagt aagggtggct atcccagttc
       61 aactggttgg tgaacaactc cagcactccg ctgccgccat actcgccagg cggcaagctg
      121 atcgagtgca gcacgatgag gtcaatgtgc gccagggcag ggcgcggacc gaagttggga
      181 gaagggcgcg gtctggcgaa ttgataccac ccacccttcc agaggcggga tgccaagacg
      241 ggtgtttcag gcatcagcct cgtcctccgc agattgtggc gtatctattt tcaaccgggc
      301 gatgcggtat cgaatctgcc gcaggctcaa ccccaggcgc gttgccgcca aagtgcgatt
      361 gaagccggtt tccttcaggg tgcgaaccag aatatcccgc tcctgctggt ccagatgggt
      421 ctgcagatcc gatggaatgt cctcagcgcg gggagcggac agaggtgctg gagtcgttgt
      481 tgcttctatt ggcgccggcc gcgttgcgat cgacatgcag tcatccaact gcagctcgtc
      541 gccatcggtc aatgccatgg cgcggtgtag caggttttcc agttcgcgca cattgccgtt
      601 gagaggtagc tgggacaact gcccaagcgc aatctcagcc agtttgggcg gactttgtcc
      661 ggattcggcg gcaatgcgct ggatcagcga ctgacacaat gccggcagat cctcgatgcg
      721 atcccgcaag ggcggaatat gaatatcgat cacgttgagc cggtaaaaaa gatcctggcg
      781 gaaacgccct gcagcgacct ctgcgggcaa atctttgtgt gttgcactca cgatacgtac
      841 gtctactggc tcttcctggc tgccgcctat agggcgaatg caccgctcct ggattgcccg
      901 caataacttg gcctgcatgg cgatagggag atccccgatt tcgtccagaa agagcgttcc
      961 accattcgcc gccttgaaga agccctctcg atcctggctg gcaccggtgt aggagccttt
     1021 tttggatccg aagaactccg cttccagcag gttctcgggt atggctccgc aattgaccgc
     1081 cacccatgga ccttcgctgc ggtggctgtt ggcgtgcagt gcgttggcta ccaattcctt
     1141 gccggttccg gattccccgc acactagcac cggagccatg ctgcgagcca ccttgacaat
     1201 ccggtctttg acaaagcgca tcgcgggcga gtcgcctgcg agcaaagaaa gcgcggcctc
     1261 gcctcccgtg gtgcgccccg tacgacgtgt tgcccgggtg tcggacgcgg atgacacttc
     1321 cttgggcctg tccgccgtgt tagtgccgtg aatcgccgct gccacaacgg agcgaaattg
     1381 cttcaggtca accggctttg tcaggtagtc aaatgcacct gccttgagag attccacggc
     1441 attctctgcc gagccatagg cagtaatgac aatgcaacgt tcggagcgtt gctggttgcg
     1501 cagatccttg agaagttctg ttcccatgcc atcgggcagg cacatatctg taatcacgag
     1561 gtcaaatcgt ccggcatgga gggcctcccg tgcctgttcg acggtgccgg cggtggtgac
     1621 ctggtatccc tcacgcaaca gtgtcagttc atacaaggta cgcaggtctg gctcgtcatc
     1681 gatgaccaga atgtgtatgt tgcttaataa agtgttggtt gccatgggct ggttttggcg
     1741 gtggcttcgt tggcggatgc tggcgtactg gcttgtgcca aggtaatgac aaattcattg
     1801 ccatctgtgc tctgtccccg tgcgattctg gacgtccgtt ggtagacaat ggtcgcgcca
     1861 tgccgttcgc agagttccct gcaaatgtag agccccagac cgctggatcg gctttccgag
     1921 gaaaagaatg gctcaaacag atgccgctct acggactgct ccatgggctg cccgtcgctc
     1981 cagacggaca gcatcgcatg ccgttcatcc agtacacggc tggaaacctg aatggcctcg
     2041 gcttgggacg tcgcatagcg gcgggcgtta tcaagcaggt tgaccaaaac ccgtcgaagg
     2101 tgctcgattt caaattgaac cagcagtgcc ggtccagaca acagggtcac caatctggga
     2161 gcttccccat tttgctgagc ccattcgtgg cagatgcgat gggtgtgttc gttcaaagcc
     2221 agtgacaggt tcgacgtctc tttcgcatga ggccctacct gtgccgcgtg caggatgtca
     2281 tcgacaatct ttcccagccg tttggcattt tgtccgatca tgtcggtgag ccgaatctgt
     2341 ctagggtctc ccagatcctc ttccagcagg gcattggctt ggctgatggc cgccagcgga
     2401 ttgcgtatct cgtgcgctac ggccgtcgac atgcggccca tactggccaa cttttctgtg
     2461 cgcatgcggg cttcgagtgc ccgaaggtct tgcagaaaga gcacgcagag actatctgct
     2521 ccaccacctt gtggggcggc cagtcgggta cgtaccttga tacgctgaac gccaagcgca
     2581 ccctgacgca acgtaacgtc ttcctcaagg ccctcgcctg ttccgataca tacgcgtgtc
     2641 aggttccaca ggggcgccca acccgcatgt gctttcaggt ccagcagatg cggctgtgcg
     2701 tgggccaccc ccagtaagcg ccgggcagca ggattggctg ccagaacccg gccacttgca
     2761 tcaacaatca cgaccccgtc ctgaagcgat tcaatcacca gttcattgac gtggcgttga
     2821 atattggcgg cagcctgatt gaggcgggcg cgttgccctg cattggccag acgggtggac
     2881 agctgattgg ccagcagtgc gataacgaag tacccaacgc cactgagtgc agattgaaca
     2941 aagtatgcag ttgcatctgc gaggctggtc aaatgcgtcc atgttgtatt gaccagaagc
     3001 agcacggtga ctcccgccgc cgtacccagg gcgaggcgta gcggccccaa tacggcagaa
     3061 agaaggacgg gtaacgcaaa caagggggtg tagttgatgt tgttgccctg gacgatttgc
     3121 aatgcagaga acgccacaac atccacaccg accaggctga gccaggattt gttgaacgaa
     3181 gttcccagag cgtggggctt ttccagtagc ctggatgcca gcgtggccac aaagtaggcg
     3241 acacagatcg caaccagcag gcggctttgt gcaaaccctg tgaagtagag cgagccttgc
     3301 agggcaacca tcactacact gagtgcgagc ctggcggtca tgaagccctg ccactgacgc
     3361 acgtactctc tggactcttc cgtactctcc aggacggccg ctatatctgg ggggccaaac
     3421 caggagtgtt gcgtggcgtc gtcgtccatt caggactccg caatctttct gtgtgcgttg
     3481 caacagtaca caccccgcgc acctggcacc gcctctccgt ccggaatgtg aatatcacaa
     3541 tgcgcgcact gcaccatttg tgtcggcgtc gccttggcac gttcgcgttg ttggcgcgtc
     3601 gctatttggt ctgcggtacg tgcagacctc cacttccagg caacgaggaa gatcaccagg
     3661 aagatcagca ggtatttcac gcggtccgtc ccagaaaaat ttccatgacg aaccgtgagc
     3721 ccacataggc gagcaaaagg aatgccgtcc cgatatacaa caccatggct gcgcgcttgc
     3781 cacgccaccc agccagccag cgcccagcca aaagtacggc gaatgtcacc caggacaata
     3841 gggaaaatac cgttttatga tcccagcgcc acgcgtggcc gtagaccact tcaccaaaca
     3901 gcatacctac cagcagggtc aggctcagca acgcaaaacc tgcagctaca aagcggtagg
     3961 tcaatcgctc cagcgtcagc aggggcaggc cactgtggga gtcctctgcc atgcggatgc
     4021 gcttttcggc gtggttcatg aacagggcat gcaccactgc catgccaaac aggccgtagc
     4081 aggcaatcgc aaaggcgaga tggacaggta gccatacaga cgctgtggct ggcagtgtgt
     4141 tgcctggaaa agcgtaggcc agcagtaccg ccgccgcgcc gagtgcacac agggtccaac
     4201 gggtggatag ttgcgggtag acaaggcttt ctacggcata gaccgccgcg accagccatg
     4261 cggtgaccga cagtgccggg gcaaatccaa aatgggcggg atcgcccatc aatcccatgg
     4321 ccagtaccag tccatgcagc agccaggcga cggccaccca cagatgcgac gtacgcaagc
     4381 tcattcgggt tgcacgcaag gcaggccaag cgtaggcaga ggccgccaaa accgaaagaa
     4441 tcagttgaac cggggatgca ctggctaaaa tcatgggcac agtttagcgt tttgcactcc
     4501 gctggctccg gccagcgttc acacaaggtt ccgttcgccg gaatacgttc tatggcctcc
     4561 gctctttccg acaaactttc ccgactcgtc aaggacctcc gcggtcaggg gcgcattacc
     4621 gaaagcaatg tgaccgacat gctgcgtgag gtgcgcatgg ccttgctgga ggccgacgtg
     4681 gccc
//
LOCUS       DAOSJM010000184         4672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16308_length_4672_cov_4.327702, whole genome shotgun sequence.
ACCESSION   DAOSJM010000184 DAOSJM010000000
VERSION     DAOSJM010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16308_length_4672_cov_4.327702"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="PKX82_07600"
     CDS             <1..707
                     /locus_tag="PKX82_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52156.1"
                     /translation="SFCMCPGGTVVAATSEPGRVVTNGMSQYSRNERNANAGIVVGIE
                     PQDYPSDEAAFVQAFGETAGKRYAQEAAALKAHDPKAAHPLSGIALQRQLEAGAYTLG
                     GSTYEAPGQLVGDFIAAHPSAQWGSVTPSYKPGVKLGDLAPSLPAYAIEAIREALPAF
                     GKKIKGFDMADAVLTGVETRTSSPLKMPRGENLQSTNVVGLYPAGEGASYAGGILSAG
                     VDGIRVAEAVARDILA"
     gene            742..1299
                     /locus_tag="PKX82_07605"
     CDS             742..1299
                     /locus_tag="PKX82_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOF52157.1"
                     /translation="MKIDPAVAKIFAENVRAEVIVRPAVSNLDPAIVQKIAGKVKVFQ
                     DMSSDCLSRTLALAERMPFVAGDVVFNEGDIGSAFHVLIAGEVVVEKKRGDTTVELAR
                     LSAGECFGEMALAGNHLRSATVRALHNSVTLRFDRERVDAYPESAHIIYRNIARVLSA
                     RLDTSSELLTEMTVRNSETRPADLR"
     gene            1427..2485
                     /locus_tag="PKX82_07610"
     CDS             1427..2485
                     /locus_tag="PKX82_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOF52158.1"
                     /translation="MNAILPGATAPADSGSTHPPLELVCPAGSLPALKAAVDNGASCV
                     YLGLRDATNARNFAGLNFDEAAIANGIAYAHQRGVKVFMALNTYPQASNPEPWRSAMD
                     KAVDLGVDAVILADPGLMLYATQRHPALRLHLSVQGSATNYDAITFYREQFGIVRAVL
                     PRVLSLTQVQQVIEKTPVEIEVFGFGSLCVMVEGRCALSSYVTGEAPNTHGVCSPPKA
                     VRWQETPQGRESRLNGVLIDRYAPGENAGYPTLCKGRFDVGNDENYYAIEEPTSLNTL
                     ELLPQLMKMGVRAIKIEGRQRSPAYVAQVTKVWREAIDHCRDNPHRYAAKPIWMSSLD
                     QVAEGQQHTLGAYHRPWK"
     gene            2500..3399
                     /locus_tag="PKX82_07615"
     CDS             2500..3399
                     /locus_tag="PKX82_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOF52159.1"
                     /translation="MKISLGPLLYYWSRESVISFYDRMANSAVDIVYLGEAVCSRRHE
                     LRLSDWLDIAAKLRQAGKEVVLSTQVLLESGAEVATMHKITGNGDFLVEANDMGAVRC
                     LSGKVPFVAGPHLNIYNLPTLEWMASLGASRWVMPLEMQRDDLAHLLQKLPPGLQTEV
                     FAYGRMPLAFSARCFTARHYNLPKDDCRFSCIQHPDGLMLKTREHEEFLVLNGTQTQS
                     ARVYNLVDTLDDMRSLGVEVVRISPQATHTEDVVAVFDAARKQALTPQAALASTQALM
                     PDQGCNGYWHGQPGLDFVACATA"
     gene            3413..3916
                     /locus_tag="PKX82_07620"
     CDS             3413..3916
                     /locus_tag="PKX82_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOF52160.1"
                     /translation="MASTTPYTLPGTIGALLSRLPAYPGSLLLVTALNQVLVPLLPQD
                     VRAILRGKKLRIHVSDARVTLDFTCDGSRFSAMPSAGTTDLTISACAHDFLRLAQRQE
                     DPDTLFFNRRLSMEGDTELGLVVKNTLDGLELPVFDLQQWTPPQVLARIMAKHRAGTG
                     AHAEGRP"
     gene            3913..4293
                     /locus_tag="PKX82_07625"
     CDS             3913..4293
                     /locus_tag="PKX82_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOF52161.1"
                     /translation="MTLQASVRPVVYSCSGCSSAAQLANHVALQLDRKGLAEMSCIAG
                     VGGDVPHLMKLARSGRPIIGLDGCPSVCVQSSLARHGIACDQHYQLQQYGVKKRKHED
                     FDADQAAQVLARIEADLQTDMVRA"
     gene            4290..>4672
                     /locus_tag="PKX82_07630"
     CDS             4290..>4672
                     /locus_tag="PKX82_07630"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HOF52162.1"
                     /translation="MSAPTRRIIVGISGASGAIYGVRLLQALREMPGVESHLVVSDAG
                     WRNVQEEHGLQRAAIEALAHQVHEVHDIGAAIASGSFQCSGMVIAPCSMRTLAAVAHG
                     LSDNLITRAADVILKERRRLVLMVR"
BASE COUNT          892 a         1520 c         1427 g          833 t
ORIGIN      
        1 acagcttctg catgtgtccg ggtggcaccg tggtggccgc caccagcgag cccgggcgcg
       61 tcgtcaccaa cggcatgagc cagtattcgc gcaacgagcg caatgccaat gcgggcatcg
      121 tggtgggcat cgaaccgcag gactaccctt cggacgaagc cgcatttgta caggcattcg
      181 gtgaaaccgc gggcaaacgt tatgcgcagg aggccgccgc gctcaaggca cacgacccga
      241 aagccgccca ccccctgtcg ggcatcgcac tgcagcgaca gctggaagct ggcgcgtaca
      301 ccctgggcgg cagcacctac gaagcgccgg gccagttggt gggcgacttc attgcggcgc
      361 atccgtcggc acagtggggc tcggtcacgc cgtcttacaa acccggcgtc aagctgggtg
      421 acctggcgcc cagtctgccc gcgtatgcca tcgaagccat ccgcgaggcg cttcccgcct
      481 ttgggaagaa gatcaagggc tttgacatgg ccgatgccgt gctcacgggc gtggaaacac
      541 gcacctcgtc gccattgaaa atgccgcgcg gcgagaacct gcagagcacc aatgtggtgg
      601 gcctgtaccc tgccggcgag ggcgccagct acgccggtgg cattctgtca gccggggtgg
      661 atggcatccg cgtggccgaa gcagtggcgc gggacattct ggcataggca aaccacagag
      721 tggggtgtac catcttcctc catgaagatt gaccctgcag ttgccaagat atttgcggaa
      781 aacgtccgcg ccgaagtgat tgtgcggcct gcggtctcga atctcgatcc agccatcgtg
      841 caaaaaattg ccggcaaggt caaagtcttt caggacatgt cctcggactg cctgtcccgc
      901 accctggcac tggccgaacg catgcctttc gtcgccggcg atgtggtctt caacgaaggc
      961 gatatcggca gcgcgttcca cgtgctcatt gcgggcgagg tggtcgtcga gaaaaagcgc
     1021 ggcgacacca cggtcgagct ggcccgcctg agcgctggcg agtgctttgg cgaaatggcc
     1081 ctggcaggca accacctgcg cagcgctacg gtacgcgcac tgcacaacag cgtcaccctg
     1141 cgttttgacc gcgagcgtgt ggacgcctac cccgaaagcg cccacatcat ctaccgcaac
     1201 atcgccagag tgctctcggc ccggctggac acgtccagtg agctgctgac cgagatgacc
     1261 gtgcgcaata gcgaaacacg cccggccgac ctgcgttgac cggaagggcc gcgcccttat
     1321 acctgaataa aacagtcaat ctttcagcga gtctgttgac agagatcaga ctcttccctt
     1381 ctgcattgtt ctatcgtgcg ggtccagcac tctcactctg gatagcatga acgcaatact
     1441 ccccggcgct actgcgcccg ccgactccgg gtccacccac ccacctttgg aattggtgtg
     1501 cccggcaggc agcctgcccg ccctgaaggc cgctgtggac aacggcgcca gctgtgtcta
     1561 tctgggcctg cgcgacgcca ccaacgcccg caattttgcc ggtttgaact ttgacgaagc
     1621 cgccattgcc aatggcattg cgtatgcgca ccagcgcggc gtcaaagtct tcatggccct
     1681 gaatacctac ccacaggcct ccaaccccga accctggcgc agtgccatgg acaaggcggt
     1741 ggacctgggc gtcgatgccg tgatcctggc cgacccgggc ctgatgctgt atgccacgca
     1801 acggcatccc gcgctgcgcc tgcacctctc ggtacagggc tctgccacca actatgacgc
     1861 catcaccttc taccgcgagc agttcggcat cgtgcgcgcg gtgctgccgc gggtgctgtc
     1921 gctgacgcaa gtccagcaag tcattgaaaa gaccccggtg gaaattgaag tctttggctt
     1981 tggcagcctg tgcgtcatgg tcgagggccg ctgcgcactg tccagctatg tcaccggcga
     2041 agcccccaac acccatggtg tgtgctcgcc cccgaaagcc gtgcgctggc aggagacccc
     2101 gcagggccgc gagtcgcgcc tgaacggtgt gctgattgac cgctacgccc ccggagaaaa
     2161 cgcgggctac ccgacgctgt gcaaaggtcg ctttgatgtg ggcaacgacg aaaactacta
     2221 cgccatcgag gagcccacca gcctcaacac cctggagctg ttgccccaac tgatgaagat
     2281 gggcgtcagg gccatcaaga tcgaagggcg ccagcgcagt cctgcctatg tggcccaggt
     2341 gaccaaggta tggcgggagg ctatcgacca ctgcagggac aacccacacc gctatgccgc
     2401 caagccgatc tggatgagca gcctggacca ggttgccgaa ggccagcaac acacgctggg
     2461 cgcctaccac cgtccctgga aatgaatgcc atgcgtcctt tgaaaatttc tcttggcccg
     2521 ttgctgtact actggtcgcg cgagtccgtt atttcgtttt atgaccgcat ggccaactcc
     2581 gccgtcgaca tcgtttacct gggcgaagcg gtctgctcgc gtcgccacga attgcgtctg
     2641 tctgactggc tcgacatcgc tgccaagctg cgccaggctg gcaaggaggt ggtgctctcc
     2701 acccaggtgc tgctggagtc gggcgccgaa gtggccacca tgcacaagat caccggcaac
     2761 ggggactttc tggtggaggc caatgacatg ggcgctgtgc gctgcctcag tggcaaggtg
     2821 ccttttgtgg ccggcccgca cctgaacatt tacaacctgc ccacactgga atggatggct
     2881 tctctgggtg ccagccggtg ggtcatgccg cttgaaatgc aacgcgatga cctcgcccac
     2941 ctgctacaga agctcccgcc ggggctgcag accgaggtat ttgcctatgg ccgcatgcca
     3001 ctggcgttct cggcgcgctg cttcactgca cgccactaca acctgcccaa ggacgactgc
     3061 cgcttcagct gcattcagca cccggatggc ctgatgctca agacccgcga acacgaagag
     3121 ttcctggtac tcaacggcac acaaacccag tcggcccgcg tctacaacct ggtggacacg
     3181 ctggatgaca tgcgcagcct cggagtggag gtagtgcgca tcagccccca ggctacgcac
     3241 acggaagacg tggtcgccgt gttcgacgcc gcacgcaaac aagccctcac gcctcaggcg
     3301 gcgctggcca gcacccaggc cctgatgccc gaccagggct gcaacggtta ctggcacggc
     3361 cagccgggac tggattttgt ggcctgcgcc accgcatgaa agactcctcg caatggcatc
     3421 caccaccccc tacaccctgc ccggcaccat tggtgccctg ctgtcccgtc tgccggccta
     3481 cccgggttca ctgctcttgg taacagcact caaccaggtg ttggtgccgc tgttgccgca
     3541 agatgttcgc gccatcctgc gggggaaaaa attgcgcatc catgtgagcg atgcacgcgt
     3601 gacgttggac ttcacctgcg acggcagccg tttctcggcc atgccgtccg ctggcacgac
     3661 cgacctcacc atcagtgcct gcgcccacga ctttttgcgg ctggcccagc gccaggaaga
     3721 cccggacacc ctgttcttca accgccgcct gtcgatggaa ggtgacaccg aactcggatt
     3781 ggtggtcaag aacacgctgg atggactgga gctgccggta ttcgacctgc agcaatggac
     3841 accaccgcaa gtgctggcgc ggatcatggc caagcaccgc gcaggtacag gggcgcacgc
     3901 agagggacgt ccatgacgct acaggcgtcc gtgcggccgg tggtgtactc ctgttctggc
     3961 tgctccagcg cggcccagct ggccaaccat gtggccctgc aactggaccg caagggcctg
     4021 gcggaaatgt cctgcatcgc gggggtcggt ggtgatgtac cgcacctgat gaaactcgcc
     4081 cgctccgggc gccccatcat cgggctggac gggtgtccgt ccgtgtgcgt tcaaagcagc
     4141 ctggcccgcc atggcattgc ctgtgaccag cattaccagt tgcagcaata cggggtgaag
     4201 aaacgcaaac acgaagactt tgacgcagac caggcggcgc aagtgctggc gcgcatcgaa
     4261 gcggacttgc aaactgacat ggtccgcgca tgagtgcacc gaccagacgc atcattgtcg
     4321 gcatctctgg tgccagtggg gccatttacg gtgtgcgcct gctgcaggcg ctgcgggaga
     4381 tgccgggcgt ggaatcccat ctggtggtgt ccgacgcggg ctggcgcaat gtgcaggaag
     4441 agcatggcct gcagcgtgcg gccattgaag ccctggccca ccaggtccac gaagtgcacg
     4501 atattggcgc ggccatagcc agtggatcat tccagtgcag tggcatggtg attgcaccgt
     4561 gctccatgcg caccctggca gccgtggccc acgggctgtc cgacaacctg atcacccgcg
     4621 cggccgatgt gatcctgaaa gaacggcggc ggctggtgct gatggtgcgc ga
//
LOCUS       DAOSJM010000185         4635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16447_length_4635_cov_4.340175, whole genome shotgun sequence.
ACCESSION   DAOSJM010000185 DAOSJM010000000
VERSION     DAOSJM010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16447_length_4635_cov_4.340175"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="PKX82_07635"
     CDS             <1..568
                     /locus_tag="PKX82_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF52163.1"
                     /translation="WVLHQRARLGFWLFAGLGASLVVGFAWMRAAQHAQGFALEWADL
                     ALIGAIISAAIGYVYGAQITPTLGAERVICWVCVLALPVTVPGTLLMWPDTPAPVSAW
                     LALLYVGVFSMWAGFFAWYRGLAIGGALKVSQVQLVQPFISILAAVPLLGEPLETMTL
                     GFTLAVVATVFAGKRFAAGSPSPRAALT"
     gene            579..917
                     /locus_tag="PKX82_07640"
     CDS             579..917
                     /locus_tag="PKX82_07640"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOF52164.1"
                     /translation="MPHPAFAQLPAPPYYAVIFSSLRTSQGDAEYGAMTDRMVELAQH
                     QPGYLGAESTRGTDGFGITVSYWSSLDAIAHWRSHAEHRVAQETGKAQWYEHYELRVA
                     LVEKAYGKQP"
     gene            956..2185
                     /locus_tag="PKX82_07645"
     CDS             956..2185
                     /locus_tag="PKX82_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOF52165.1"
                     /translation="MPTTPTHWTLAARASKMNPSVIREILKVTEKPGIISFAGGLPSS
                     KTFPVAEFDAACAKVLRDDPAGALQYAASEGYGPLRELVASQLSAQSAAAGVPWNVDP
                     AQVLITTGSQQGLDLVAKILIDAGSKVLVESPTYLGAVMAFTPMEPNVVSVASDDDGI
                     DIADLAAKSADARFLYVLPNFQNPTGRSMSEARRAALSAEALRSGLPIMEDNPYGDLW
                     FDQAPPAPLTARNPEGGIYLGSFSKVLAPGLRMGFLVAPKAVYPKLLQAKQAADLHSP
                     SFNQRIIFEVMQNGFLDRHVPTIRALYKTQRDAMLQALSAHFPDAATQPDTSLTWNTP
                     AGGMFLWARLPAGMSAVELLPHAVDKGVAFVPGAPFYADHGDPRTMRLSFVTPSVEEI
                     QRGVAALAAAIAERRRA"
     gene            2178..3125
                     /locus_tag="PKX82_07650"
     CDS             2178..3125
                     /locus_tag="PKX82_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOF52166.1"
                     /translation="MHSRPFKQVDVFTDRPYLGNPLAVVLDGTGLSDAEMQRFARWTN
                     LSETTFVLPPTPDAAAQGADYQVRIFTPAYEMPFAGHPTLGSCHAWLEHDGSPRNRDE
                     IVQQCKVGLVRIRRTGKRLAFAAPPLQRHDPTREVASKVAAALGLSKTHVLAMQHLTN
                     GPKHFGVLVDTIEHVLELQPNFAALQTLMPDMRVTGIGVVALASESDAPALITRSNRE
                     ARAFGASAATDTAIPAEAEVRFFFTGNRLVSEDMVTGSFNASVAQWLMTDSLAPRDYV
                     AAQGTCIGCEGRVHLSMDDAGQVWVGGDAVTCISGMVNL"
     gene            3122..4471
                     /locus_tag="PKX82_07655"
     CDS             3122..4471
                     /locus_tag="PKX82_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOF52167.1"
                     /translation="MTAPQTPSAPPPRFLTGSLLRHIVVMAGTGAIGLVAIFAVDLIN
                     LLYISMLGEQAIAAAVGFAGVVGFFHMSIYIGLLIGITACVSRAVGAHQTDTAHALGT
                     SSLVITAAIGVALTLVTLVFLQPILQGLGAEGRVLELAYRFVLITTPSTILLGLGMAC
                     SALLRSVGDARRSMMVTLVPAIVTAVLDPVFIFVFGLGLDGAAIVVVIARMVLLAVGL
                     WGAWKVHKLLARFQRSRLRRDAKTLFTVAGPAILTNLATPVGAAFVTHSIAGFGASAV
                     AGQATIERLTPVAFGLIYSLSGAVGPVLAQNLGAKRYDRVREGLRASLWFMVVSVAAA
                     WLILALVQGPLIRAFSLDGVAAAMVHAFCSWIAASFFFMGALFVANAAFNNLGRPLWS
                     TGFNWGRATLGTIPFAWWGAQYGPVEVLAGQAAGAAIFGTLALVCAFWLTKTLEHSR"
BASE COUNT          723 a         1620 c         1465 g          827 t
ORIGIN      
        1 ctgggtgctg caccagcgcg cgcggctggg gttctggctg tttgcgggcc tgggtgccag
       61 tctggtggtc ggcttcgcct ggatgcgcgc cgcgcagcac gcacagggct ttgcactgga
      121 atgggccgac ctggcgctga tcggggccat catttcggcc gccatcgggt atgtatacgg
      181 cgcacagatt acgcccacac tgggtgccga gcgggtgatc tgctgggtgt gcgtgctggc
      241 actgccggtc accgtgccgg gaaccctcct gatgtggccc gacacgccgg ctccggtcag
      301 cgcctggctg gccctgctgt acgtgggcgt gttttctatg tgggctggtt tttttgcctg
      361 gtaccgcggg ctggccattg gcggcgcgct caaggtcagc caggtacaac tggtccaacc
      421 cttcatcagc atcctggctg ccgtgcccct gctgggcgaa ccgctggaaa ccatgacgct
      481 ggggttcacc ctggcggtgg tggccacggt attcgccggc aagcgctttg ccgcaggcag
      541 cccttccccg cgcgcggcgc tgacgtgaca atagacccat gccccaccct gcttttgccc
      601 agttgcccgc cccgccttac tacgcggtga ttttttcttc cttgcgcact tcgcagggcg
      661 acgccgaata cggggccatg accgaccgta tggtggagct ggcgcaacac cagcccggct
      721 acctgggcgc agaaagcacc cgcggcaccg atggctttgg catcaccgtg tcgtactgga
      781 gcagtctgga cgccattgcc cactggcgct cccatgccga gcaccgggta gcgcaggaaa
      841 ccggcaaggc acagtggtac gaacactatg aactgcgtgt cgccctggtc gaaaaggcct
      901 acggcaaaca accctgacaa tttccagcca agaagcaagc caagagaaaa cgcccatgcc
      961 caccacaccc acccactgga ccctggccgc acgtgcctcc aagatgaacc cctcggtgat
     1021 ccgcgagatc ctcaaggtca ccgagaagcc cggcatcatc agctttgctg gcggcctgcc
     1081 atcgagcaag accttccctg tggccgagtt cgatgcggcc tgcgccaagg tgctgcgcga
     1141 cgacccggct ggcgccctgc agtacgccgc cagcgagggc tatggcccgc tgcgcgaact
     1201 ggtcgcttcg cagctcagcg cccagagcgc tgccgcaggc gtaccgtgga acgtagaccc
     1261 ggcgcaggtg ctcatcacca ccggctcgca acagggcctg gacctggtcg ccaaaatcct
     1321 gatcgacgcc ggtagcaagg tgctggtgga atcgcccacc tacctgggtg cggtgatggc
     1381 cttcaccccc atggaaccca acgtggtgag cgtggcctcg gacgacgacg gcatcgacat
     1441 tgccgacctg gccgccaaat ctgcggatgc gcgcttcctg tacgtgctgc ccaacttcca
     1501 gaacccgacc ggacgcagca tgtccgaagc ccgccgcgct gcactgagcg cagaggcact
     1561 gcgcagcggc ctgcccatca tggaagacaa cccctatggc gacctgtggt tcgaccaggc
     1621 accgccagcc ccgctgacgg cgcgcaaccc tgaaggcggc atttacctgg gctcgttctc
     1681 caaggtgctg gcgccaggcc tgcgcatggg ctttctggtc gcacccaagg cggtgtaccc
     1741 caagctgctg caggccaagc aggccgccga cctgcatagc cccagcttca accagcgcat
     1801 catttttgaa gtgatgcaaa acggtttcct ggaccgccac gtgcccacca tccgcgcgct
     1861 gtacaagacc cagcgcgatg ccatgctgca ggcactcagc gcccacttcc ccgatgccgc
     1921 cacgcagccc gacacctccc tgacatggaa caccccggcc ggcggcatgt tcctgtgggc
     1981 ccggctaccc gcaggcatga gcgcggtgga actgctgccg catgccgtag acaaaggcgt
     2041 ggcgtttgtg cccggtgccc cgttctatgc ggaccatggc gacccgcgca ccatgcgcct
     2101 gtcgtttgtg acccccagcg tggaagaaat ccagcgcggc gtggccgcac tggccgccgc
     2161 cattgcggag cgccgccgtg catagccgtc cgttcaaaca ggttgatgtc ttcaccgacc
     2221 ggccctacct gggcaacccg ctggccgtgg tgctggacgg cacgggcctg tccgacgccg
     2281 agatgcagcg gtttgcccgc tggaccaacc tgagcgaaac cacctttgtg ctgccgccca
     2341 cgcccgacgc cgcagcccag ggggccgact accaggtgcg catcttcaca ccggcctacg
     2401 aaatgccgtt tgccggccac cccacgctgg gcagctgcca cgcctggctg gaacacgacg
     2461 ggtccccccg caaccgcgat gaaatcgtcc agcaatgcaa ggtggggctg gtccgcatcc
     2521 gccgcaccgg caaacggctg gcctttgccg cgccacccct gcaacgccat gacccgacac
     2581 gcgaagtcgc cagcaaggta gccgctgcac tggggctgtc caagacccat gtgctggcga
     2641 tgcagcacct gaccaacggc cccaaacatt ttggtgtcct ggtcgacacc atcgagcacg
     2701 tgctggaact ccaacccaat ttcgccgcac tgcaaaccct gatgcccgac atgcgggtca
     2761 ccggcattgg cgtggtggca ctggcgtccg aatcggatgc cccagcgctg atcacccggt
     2821 ccaaccggga ggcccgtgcc ttcggtgcgt ccgcggcgac cgacaccgcc attcctgccg
     2881 aagcggaggt gcgcttcttc tttaccggca accggctggt gtccgaagac atggtcactg
     2941 gcagcttcaa tgccagcgtg gcgcagtggc tgatgaccga cagccttgcg ccgcgcgact
     3001 acgtggccgc ccagggcacc tgcatcgggt gcgagggccg ggtccacctg tcgatggacg
     3061 atgcaggcca ggtctgggtc ggcggcgatg cagtgacctg catctcgggt atggtcaacc
     3121 tatgaccgcc ccccagacgc cttctgcacc acccccacgc tttctcaccg gctccctgct
     3181 ccggcacatc gtcgtcatgg cgggcaccgg cgccatcggg ctggtggcca tctttgcggt
     3241 ggacctgatc aacctgctct acatctccat gctgggcgag caggccattg cggcggcggt
     3301 gggctttgcc ggcgtcgtgg ggttcttcca catgtcgatc tacatcggcc tgctgattgg
     3361 catcaccgcc tgcgtttcgc gcgcggtcgg cgcccaccag accgacaccg cccacgcgct
     3421 gggcacctcc agcctggtca ttaccgcggc tatcggtgtc gcgctgacgc tggtgacgct
     3481 ggtgtttctg cagccgatcc tgcagggtct gggggctgaa ggccgggtgc tggagctggc
     3541 ctaccggttt gtactgatca ccacgccatc gaccattctt ctggggctgg gcatggcctg
     3601 ctcggccctg ctgcgctcgg taggcgacgc gcgccgctcc atgatggtga cgctggtgcc
     3661 cgccatcgtc accgcggtgc tggacccggt gttcatcttt gtcttcggcc tggggctcga
     3721 cggtgcggcc atcgtcgtgg tcattgcacg catggtgctg ctggcagtgg gcttgtgggg
     3781 cgcctggaag gtgcacaagc tgctggcccg cttccagcgc agccgcctgc ggcgcgatgc
     3841 caagacgctg ttcaccgtgg ccgggccggc cattctgacc aacctggcca cgccggtcgg
     3901 cgctgcgttt gtcacccaca gcattgcggg ctttggtgcc tccgccgtgg cgggccaggc
     3961 caccatcgag cggctgaccc ccgtggcatt cggtctgatc tattccctca gcggtgccgt
     4021 cggcccggtg ctggcgcaaa acctcggcgc caaacggtat gaccgggtgc gcgaaggcct
     4081 gcgcgccagc ctgtggttca tggtggtgtc cgtggccgca gcctggctga tcctggcgct
     4141 ggtgcagggc ccgctgatcc gcgcgttctc gctggacggc gtggcggccg ccatggtgca
     4201 cgccttttgc agctggatcg ccgccagctt cttctttatg ggcgcactgt ttgtggccaa
     4261 tgccgcgttc aacaacctgg ggcgcccgct gtggtccacc ggcttcaact ggggccgcgc
     4321 cacgctgggc accattccgt ttgcctggtg gggcgcccag tacggcccgg tcgaagtgct
     4381 ggccggccag gccgccgggg ccgccatctt tggcacgctg gcgctggttt gcgccttctg
     4441 gctgaccaag accctggagc attcccgatg aacctgccca ccctgcccga gatcgaagcc
     4501 gctgcccagg tggtgtaccg cgagtttgcc gccacaccgc aataccactg gggcctgctg
     4561 gccgaaaagc tgggcactgc ctgctgggtc aagcacgaga accacacgcc ggtgggcgcc
     4621 ttcaagatcc gtggc
//
LOCUS       DAOSJM010000186         4630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16472_length_4630_cov_4.408962, whole genome shotgun sequence.
ACCESSION   DAOSJM010000186 DAOSJM010000000
VERSION     DAOSJM010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16472_length_4630_cov_4.408962"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..418)
                     /gene="ybgC"
                     /locus_tag="PKX82_07660"
     CDS             complement(20..418)
                     /gene="ybgC"
                     /locus_tag="PKX82_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HOF52168.1"
                     /translation="MSDRSNPLTVRVYWEDTDAGGVVFYANYLKFFERARTEWLRALG
                     VEQTTLKADTGAIFVVAEVNLRYLAAAKLDDLLIITVKVLDQGAASLELEQQAWRGDT
                     LLAEGRVRVGCVDSTTLRPRRMPKQVLDAL"
     gene            complement(411..803)
                     /locus_tag="PKX82_07665"
     CDS             complement(411..803)
                     /locus_tag="PKX82_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HOF52169.1"
                     /translation="MTGWVWPDREVIIAVHEMQLAEHGGLAGVRDVGLLDSALSRAPN
                     LAAYGDADAAALAAAYGYGLSRNHPFIDGNKRTGFVAAELFLNLNGFELNATDADCVI
                     TMLGVAAGEVSEDAFAAWLRAHTEKRHV"
     gene            complement(800..1024)
                     /locus_tag="PKX82_07670"
     CDS             complement(800..1024)
                     /locus_tag="PKX82_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOF52170.1"
                     /translation="MHVLKLTQIGNSVGAVFPRELLNQLQLEKGDALYVTESPDGLRI
                     TKHNPEFAEQMQAARDIMKRRRDVLHELAK"
     gene            complement(1058..1843)
                     /locus_tag="PKX82_07675"
     CDS             complement(1058..1843)
                     /locus_tag="PKX82_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOF52171.1"
                     /translation="MAEPVSITSRDNPLLKELRKVAQDNTAYRKVGRFWVEGDHLCSA
                     ALQRGVKPAAAVYSESFWQNPKSRPLAPVEYARAAIKNIVIPDGLFRELSGLESPASV
                     GFVIDLPDDVSLQPLVPTVILDRVQDAGNVGSILRSAGAFGFRQVLALKGTAALWSPK
                     VLRAGMGAHFGLTLLEGLTIEDLQALQVPIVVTSSHQGDLIQNLKLPHPCAWAMGHEG
                     QGVCAELMQRAAVHVRIGQPGGEESLNVAAAAAICLHASSIQR"
     gene            complement(1852..2496)
                     /gene="rnhB"
                     /locus_tag="PKX82_07680"
     CDS             complement(1852..2496)
                     /gene="rnhB"
                     /locus_tag="PKX82_07680"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOF52172.1"
                     /translation="MPSKKSSRVKAPPTEQVAFVFDLPGLVAGVDEAGRGPLAGPVVA
                     AAVILDDLKPIKGLNDSKKLTAKQRDKLYDEIRAKALCCSIAQASVEEIDDINILQAT
                     MLAMKRAVEGLRLKPAKVLVDGNRLPVLDVLAEAIVKGDATVPAISAASILAKVTRDR
                     WCAELDAQYPQYGFAGHKGYGTAEHLAALQKHGACPEHRKTFSPVTQVLAQAFV"
     gene            complement(2468..3643)
                     /gene="lpxB"
                     /locus_tag="PKX82_07685"
     CDS             complement(2468..3643)
                     /gene="lpxB"
                     /locus_tag="PKX82_07685"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOF52173.1"
                     /translation="MSSGVPGGKAPTIAMVAGEASGDLLAGLLLGGLKDRWPELQSHG
                     IGGPQMAKHGFAAWWPHHKLSVHGFSWELLRRYREIVGIRKQLKERLLAQRPDVFIGV
                     DAPDFNLDLEADLKAAGIKTVHFVSPSVWAWRAKRLEKMRRSVDHVLCIFPFEPELLA
                     AHGIEATYVGHPIASIIPMVPNKASARSVLGLEPSDTVVALLPGSRPGEIRHLALRFF
                     KAAALVKAVYPATKFIVPAVPGLREEIEAIAQRSGMAPHLQIVTGQSHIVLAACDVTL
                     IASGTATLEAALFKRPMVIAYHMGWVSWQLMQRKRLQPWVGLPNILCRDFVVPELLQS
                     AATPEALAHGVLNWLDSAHRNPAKIAELQETFTTLHQTLQRDTATLATHAIEKVLQG"
     gene            complement(3652..4446)
                     /gene="lpxA"
                     /locus_tag="PKX82_07690"
     CDS             complement(3652..4446)
                     /gene="lpxA"
                     /locus_tag="PKX82_07690"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOF52174.1"
                     /translation="MSNIHSTAIVDPAAQIDPSVTIGPYTVIGPHVRIAAGTTVGPHC
                     VIEGHTTIGRDNRIFQFNSLGAIPQDKKYAGEPCELVIGDRNTIREFCTFNIGSPGDA
                     GVTRVGNDNWIMAYVHLAHDCHVGNNTIFANNSQLAGHVQVDDWVILGGFTVVHQFVR
                     VGAHSMTAMCSLLFADLPPFVMCQGQPAAARSMNFEGLRRRGWTPERISGVKAIHKAL
                     YRDDLTLEQAKTRIAELAVAQPVTEPDVTMMLSFLDKTTAQRGIVR"
     gene            complement(4460..>4630)
                     /locus_tag="PKX82_07695"
     CDS             complement(4460..>4630)
                     /locus_tag="PKX82_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabZ"
                     /protein_id="HOF52175.1"
                     /translation="IDGARFKRPVEPGDQLILNVEMLRNKAGIFKFKASATVGTELAC
                     EAELTCAMRTIA"
BASE COUNT          880 a         1425 c         1466 g          859 t
ORIGIN      
        1 gagggcctag cgttttccct tacagggcgt ccaaaacttg ctttggcatg cgccgcggac
       61 gcagggttgt gctgtccacg cagcctaccc gtactctacc ctcggccagc aaggtgtcac
      121 cgcgccaggc ctgctgctcc agctccagcg acgcagcgcc ctggtccagc accttgacgg
      181 tgatgatgag caggtcgtcc agtttggctg ccgccagata gcgcagattg acctcagcca
      241 ccacaaagat ggcaccggtg tcggctttca gggtggtttg ctccacgccc agggcgcgca
      301 gccactcggt acgggcgcgc tcgaagaact tgaggtaatt ggcgtagaag accacgccac
      361 cggcatcggt gtcttcccag tagacgcgga ccgtgagggg attgctgcga tcagacatgg
      421 cgtttttcgg tgtgggcgcg cagccaggcg gcgaaggcgt cttcggacac ttcgcctgcg
      481 gccacaccca gcatggtgat gacgcagtct gcgtcggtgg cattcagctc aaagccattc
      541 aaattgagga acaattcagc cgccacaaag cccgtgcgct tgtttccgtc gataaacgga
      601 tgattgcggg acaagccata gccgtaggcg gcagccaggg ccgccgcatc ggcgtctcca
      661 tacgccgcca gattgggcgc ccgggacaag gccgaatcaa gcaggccaac atcacgcaca
      721 cccgccaacc cgccgtgctc ggccaactgc atctcgtgca ccgcgatgat gacctcgcgg
      781 tcaggccaga cccagccggt cacttggcca gctcatgcaa cacgtcccgt ctgcgcttca
      841 tgatgtcgcg cgcagcctgc atctgctcgg caaattcggg gttgtgtttg gtgatgcgta
      901 gcccatcggg cgactcggtg acgtacaggg cgtcgccttt ttccagttgc aactggttca
      961 gcagttcccg ggggaacact gctccgaccg aattcccaat ttgcgtgagt ttgaggacat
     1021 gcatggcatg gctccagatg ttattacagg tgtaatacta acgctgaatg ctgctggcat
     1081 gcaagcagat agccgcggcg gcggccacgt tgagggattc ctcgccgcca ggctggccga
     1141 tgcgcacatg caccgcagcg cgctgcatca gctcggcgca gacgccctgc ccttcgtggc
     1201 ccatggccca ggcgcaggga tgcggcaatt tcaggttctg gatcaggtcg ccctgatggg
     1261 agctggtcac cacgataggc acctgcaagg cttgcaggtc ttcaatcgtc aggccttcca
     1321 gcaaggtcag cccgaaatgc gcgcccatgc cggcgcgtag caccttcggg ctccacaagg
     1381 cggcggtgcc cttgagtgcc agcacctgcc ggaatccaaa agcccccgcg ctgcgcaaga
     1441 tggagcccac gttgccggca tcctgcaccc ggtccaggat gacggtgggc accagcggct
     1501 gcaaggacac gtcgtcaggc aggtcgatga caaaaccgac gctggcgggc gactccaggc
     1561 cgctcaattc acggaacaga ccatccggaa tcactatgtt tttgatagct gctcgcgcat
     1621 attccacggg ggctagaggc ctggacttgg ggttttgcca aaaagactct gaatacacgg
     1681 ccgcagcggg cttcacaccg cgttgcaggg cggcactgca cagatggtcg ccctccaccc
     1741 agaagcggcc gaccttgcgg taggcggtgt tgtcctgggc gactttgcgc agctccttga
     1801 gcagcgggtt gtcgcgggag gtgatggaga cgggttcggc catgggcaat gtcagacaaa
     1861 cgcctgggcc aagacttggg tcactgggct gaaggtcttg cgatgctcgg ggcaggcgcc
     1921 gtgcttctgc agcgcagcca ggtgctctgc agtgccatag cccttgtggc cagcgaagcc
     1981 atattgcggg tactgggcgt ccagctcggc gcaccagcgg tcgcgggtca ccttggccag
     2041 gatggaggcg gccgagatgg cgggcacggt ggcgtcgccc ttcacgatag cttcggccag
     2101 cacatccagc accggcaggc ggttgccatc aaccagcact ttggcgggct tgaggcgcaa
     2161 gccctccacc gcgcgcttca tggcgagcat ggtggcctgc aggatgttga tgtcgtcaat
     2221 ttcttccaca ctggcctggg cgatggagca gcacagggcc ttggcgcgga tttcgtcgta
     2281 cagcttgtct ctttgcttgg cggtgagctt cttggagtcg ttcagtccct tgatgggctt
     2341 gaggtcgtcc agaatcacag ctgccgccac cactggcccc gccagcgggc cgcgcccagc
     2401 ctcatccaca ccggccacca gacccggcag atcaaagaca aacgccactt gttcagttgg
     2461 cggggcttta accctggagg actttttcga tggcatgggt ggcaagggtg gcggtgtcgc
     2521 gctgcagcgt ctggtgcagc gtggtgaagg tttcctgaag ctcggctatt ttcgcagggt
     2581 tgcggtgtgc agagtccagc cagttcagca caccatgggc cagcgcctcg ggcgtggcgg
     2641 cgctttgcag caattcgggc accacgaaat cacggcacag gatgttgggc aggcccaccc
     2701 agggctgcag gcgtttgcgc tgcatgagtt gccacgacac ccagcccatg tggtacgcaa
     2761 tgaccatggg gcgcttgaag agcgcagctt ccagcgtggc ggtgccgctc gcaatcaggg
     2821 tgacgtcgca ggcggccagc acgatgtggg actggccagt gacaatctgc aggtgcggcg
     2881 ccatgccgct gcgctgcgcg atggcttcaa tctcctcgcg caggcccggc accgcaggca
     2941 ctatgaattt ggtagcaggg tatacagcct tgacgagggc tgcagcctta aagaaccgca
     3001 aggccaggtg gcgaatctcg ccaggacggc taccaggcag cagtgccacc acggtgtccg
     3061 aaggctccag gcccagcaca ctgcgggctg aggctttgtt gggcaccatg ggaatgatgc
     3121 tggcaatggg gtgaccgacg taggtggctt caatgccgtg ggctgccagc aattccggct
     3181 caaacgggaa gatgcacagc acatggtcca cactgcggcg catcttctcc agccgcttag
     3241 cgcgccaggc ccacaccgag ggcgacacaa aatgcacagt cttgatacca gccgccttga
     3301 ggtccgcttc cagatcaagg ttgaagtccg gcgcatcgac gccaatgaac acatcgggcc
     3361 gctgggccaa caaccgttcc ttgagctgtt tgcggatgcc cacaatctcg cggtagcggc
     3421 gcagcaactc ccagctgaag ccatgcaccg aaagcttgtg gtgcggccac caggcggcaa
     3481 aaccatgttt ggccatctgc gggccaccaa tgccgtggga ttgcagctcc ggccagcggt
     3541 ccttgaggcc gcccaggagc agccctgcca gcaggtcgcc cgatgcttcg cccgcgacca
     3601 tggcaatggt cggtgctttc cccccgggca ccccgctgga catgctcggg cctagcgaac
     3661 gatgccgcgc tgcgcggtgg tcttgtccag aaaggacagc atcatggtga catccggctc
     3721 agtgaccggc tgcgctacgg ccagttcggc aatacgcgtc ttggcctgct ccagcgtgag
     3781 gtcatcacgg tacagcgcct tgtggatcgc cttgacaccg gagatgcgct cgggcgtcca
     3841 gccgcgacgg cgcagacctt caaagttcat ggagcgcgca gcggcaggtt gcccctggca
     3901 catgacaaac gggggtagat cggcaaacag cagcgagcac atggcggtca tgctgtgggc
     3961 acccacgcgc acgaactggt gcaccacggt aaagccgccc agaatgaccc aatcgtccac
     4021 ctgcacatgg cccgccagct gcgagttgtt ggcgaagatg gtgttgttgc cgacgtggca
     4081 atcgtgtgcc aggtgcacgt aagccataat ccagttgtcg ttgccgacac gggtcacacc
     4141 agcgtcgccc ggggaaccaa tattgaaggt acaaaactcg cgaatggtgt tgcgatcgcc
     4201 gatgaccagc tcgcagggct cgcctgcgta cttcttgtct tgcggaatgg cgcccagcga
     4261 gttgaactgg aagatgcggt tgtcgcgccc aatggtcgta tggccttcaa tcacgcagtg
     4321 cgggcctacg gtagtgccag cggcaatcct gacatggggg ccaatgaccg tatacggtcc
     4381 aatggtgacc gacggatcaa tctgggccgc aggatcgacg atggccgtgg agtggatatt
     4441 gctcacagcg cgtctcgggt caggcaatgg tgcgcatggc gcaggtcaat tctgcttcgc
     4501 atgccaactc cgtaccgaca gtagcgctgg ccttgaactt gaaaataccg gccttgttgc
     4561 gcaacatttc cacattcagg atgagctggt cacctggctc cacgggacgc ttgaatcggg
     4621 caccatcaat
//
LOCUS       DAOSJM010000187         4625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16495_length_4625_cov_4.562582, whole genome shotgun sequence.
ACCESSION   DAOSJM010000187 DAOSJM010000000
VERSION     DAOSJM010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16495_length_4625_cov_4.562582"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PKX82_07700"
     CDS             complement(<1..964)
                     /locus_tag="PKX82_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOF52176.1"
                     /translation="MSGHTPLPATPIELPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     AAMEAERRIMLVAQKAAAKDDPQVSDMFDVGCVSTILQMLKLPDGTVKVLVEGHQRAA
                     VNKIDEGESHFTANVTPIEVLADESDKGKTKASEVEALRRAVMQQFDQYVKLNKKIPP
                     EILTSISSIDDAGRLADTIAAHLPLKLDAKQAVLSLSEVKDRLENLFEQIEREVDILN
                     VDKKIRGRVKRQMEKNQRDFYLNEQVKAIQKELGEGEDGADIEEIEKKIKAAKMPKEA
                     LKKAEAELKKLKLMSPMSAEATVVRNYIDVLTGLPWTAKTKIKHN"
     gene            complement(1107..2372)
                     /gene="clpX"
                     /locus_tag="PKX82_07705"
     CDS             complement(1107..2372)
                     /gene="clpX"
                     /locus_tag="PKX82_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOF52177.1"
                     /translation="MAEKKGSSSEKTLYCSFCGKSQHEVKKLIAGPSVFVCDECIELC
                     NEIIRDELPATTEGKDARGDLPTPAEIKANLDNYVIGQEPAKRTLAVAVYNHYKRLRH
                     KEKAKKDDVELAKSNILLIGPTGSGKTLLAQTLARMLDVPFVMADATTLTEAGYVGED
                     VENIVLKLLQSCNYEVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLI
                     EGTMASVPPQGGRKHPNQDFVQIDTTNILFICGGAFAGLEKVIENRTQSSGIGFGATV
                     QSKQQRSLTEVFKDVEPEDLIKFGLIPELVGRMPVVATLAELTEDALIQILTEPKNAL
                     VKQYAKLLAMEGAELEIRPSALKAIAKRALARKTGARGLRSILEQSLIDTMYELPNVA
                     NVEKVVVDESTIEENKPPLLVYREAAKKA"
     gene            complement(2478..3107)
                     /gene="clpP"
                     /locus_tag="PKX82_07710"
     CDS             complement(2478..3107)
                     /gene="clpP"
                     /locus_tag="PKX82_07710"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOF52178.1"
                     /translation="MNVKYGAPGAMDIQGLGMVPMVIEQSGRGERSYDICSRLLKERV
                     IFLVGPVNDQTANLVVAQLLFLESENPDKDISFYINSPGGSVSAGMAIFDTMNFIKPD
                     VSTLCTGMAASMGAFLLAAGAKGKRFSLPNSKVMIHQPSGGSQGQATEIEIAAREILK
                     TREQLNKILADRTGQPLDRIAKDTERDYYMTADEAKEYGLVDQVISKRP"
     gene            complement(3242..4552)
                     /gene="tig"
                     /locus_tag="PKX82_07715"
     CDS             complement(3242..4552)
                     /gene="tig"
                     /locus_tag="PKX82_07715"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOF52179.1"
                     /translation="MAVTVETLEKLERKITLTLPVGVIQTEVDSRLRKLARTVKMDGF
                     RPGKVPMNVVAQRYGYSVHYEVMNDKVGEAFAAAANEAKLRVAGQPRISENETAPEGQ
                     MAFDAVFEVFPEVKIGDLTTAEVEKINTEVSDAAIDKTVEILRKQRRTFAQRALEAAA
                     QDGDRVTVDFEGKIDGEPFAGGKAEDFQFIVGDGQMLKEFEDATRGMKSGESKTFQLA
                     FPADYHGKDVAGKTADFLVTVKKIEAAHLPEVDGTLAKSLGIAEATVEGLRADIRKNL
                     EREVKYRLLGRNKNAVMDALIAKSELDLPKSSVQAELDRMVEGARADLKQRGIKDADK
                     APIPDDIFRPQAERRVRLGLVVAELVRSNNLQATPEQIKAHIDELAASYEKPADVVRW
                     YYGDNRRMAEVEAIVIENNVTDFVLSKAKVSEKSVSFDDLMGQN"
BASE COUNT          924 a         1349 c         1244 g         1108 t
ORIGIN      
        1 ggttgtgctt gatcttggtt tttgcggtcc agggcagccc cgtcaacaca tcaatgtagt
       61 tgcgcaccac ggtggcttcc gccgacatag gcgacatcag cttgagcttc ttcagttcgg
      121 cctcggcctt cttcaatgcc tctttaggca tcttggcggc cttgatcttc ttctcgattt
      181 cctcaatgtc cgcaccgtct tcaccttcgc caagctcctt ctggatcgcc ttgacctgtt
      241 cgttgaggta gaaatcgcgt tggttttttt ccatctgccg ctttacgcga ccacggatct
      301 tcttgtctac attgagaatg tccacttcgc gttcgatctg ctcgaacaga ttctccagcc
      361 gatccttcac ctcggacaag ctgaggacgg cttgcttcgc atcgagtttc agcggaaggt
      421 gggccgcgat ggtgtctgcc agccgcccgg catcgtcgat gctggaaatg gaagtcagaa
      481 tctcgggcgg aatcttcttg ttcagtttga cgtactggtc aaactgttgc atgacagcac
      541 ggcgcagagc ttcgacttca ctggccttgg tcttgccctt gtcagactcg tccgccagaa
      601 cttcgatggg ggtcacgttg gccgtaaagt gcgattcgcc ttcgtcgatc ttgttcaccg
      661 ccgcgcgctg atggccttcc accagaactt tcacggtgcc atcgggcagc ttgagcattt
      721 gcaggatggt cgacacgcaa ccgacatcga acatgtcaga cacctgagga tcgtccttgg
      781 ccgcagcttt ctgagccacc agcatgatgc ggcgctccgc ttccatggcg gcctcaagcg
      841 ccttgatgct tttgggtcga cccacaaaca gtggaatgac catatgggga aacaccacca
      901 catcacgcaa gggcaacagg ggcaattcga tgggggtagc aggtaggggg gtgtggccag
      961 acatgggata cctttttgtt acctgttgaa tatggtccgt agcgcctgaa attcaagcta
     1021 ccaggaatta tcaaacgggt cgttccagga aatattcagt taaacctacc caaaaccatg
     1081 catatccggg atttgggtag gcttggttag gcctttttgg ccgcttcgcg gtaaaccagc
     1141 agaggaggct tgttttcttc aatcgtggat tcgtccacga cgactttctc aacgttggcc
     1201 acgttcggca gttcgtacat ggtatcgatc agggactgct ccagaatcga acgcagtcca
     1261 cgggcgccgg ttttgcgcgc cagcgcacgt ttggcaatcg ccttgagtgc ggacggacga
     1321 atttccagct cggcaccttc catggccagc agcttggcat actgcttgac caaagcgttc
     1381 ttgggctcag tcagaatctg aatcaaggcg tcttccgtca gctcggccaa ggtcgccacg
     1441 acgggcatcc ggcccaccag ctccgggatc agaccaaact taatcagatc ttccggctca
     1501 acgtccttga acacttccgt gaggctgcgc tgctgcttgc tttgtaccgt ggcaccaaaa
     1561 ccaataccag acgattgcgt acggttttca atgacctttt ccaagcccgc aaatgcaccg
     1621 ccacaaataa acagaatgtt agtggtgtcg atctggacga agtcctgatt ggggtgcttg
     1681 cgcccgccct ggggcggcac gctggccatg gtgccttcaa tcagtttcag caaggcttgc
     1741 tgcacgccct cgcccgacac atcccgcgtg atggaaggat tgtcggattt gcgcgaaatc
     1801 ttgtcgattt cgtcgatata cacaataccg cgctgggcac gctccacctc gtaattgcag
     1861 ctctgcaaca acttcagaac gatgttttcc acatcttcac ccacataacc ggcttcggtc
     1921 agggtggtgg cgtcggccat cacaaaaggc acatccagca tgcgtgccag tgtttgcgcc
     1981 agcagggtct tgcccgagcc tgtgggcccg atcagcagga tgttgctctt ggcaagttcc
     2041 acatcgtcct tcttggcctt ttccttgtgc cgaagacgtt tgtagtggtt gtataccgcg
     2101 acggccaacg tacgcttggc gggctcttgc ccgataacgt aattgtccag attcgccttg
     2161 atttctgcag gtgtcggcag gtctccccgc gcatccttgc cttctgttgt ggctggcaac
     2221 tcgtcacgaa tgatctcgtt gcagagctca atacactcat cacacacaaa aaccgatggg
     2281 ccagcgatca gctttttgac ttcatgctgg cttttgccgc aaaaagagca atacaaagtt
     2341 ttttcgctgg aagagccttt tttctcggcc atgtagagag tgcctttacg ctgatatagc
     2401 ttaatgataa ccaaataaaa acggtgcccc tttttctggc ggcaccgtca tgggttgggt
     2461 tttcccggac ccggtactta cggacgcttg gagatcacct gatccaccag gccatattcc
     2521 ttggcttcgt cagcggtcat gtagtagtcg cgctcggtgt ccttggcaat gcggtccagg
     2581 ggctggccag tgcggtcagc cagaattttg ttgagctgct cgcgggtctt gaggatctcg
     2641 cgggcggcaa tttcaatttc ggtcgcctgg ccttggctac cgcccgaagg ctggtgaatc
     2701 atgaccttgg agttgggcaa agagaaacgc ttgcccttgg cacccgctgc cagcaggaag
     2761 gcccccatgc tggcggccat cccggtgcac agcgtggaaa cgtcgggctt gatgaaattc
     2821 atggtgtcaa aaatcgccat gcctgcgctc accgagccgc cggggctgtt gatgtagaaa
     2881 gaaatgtctt tgtcgggatt ttcgctttcc aggaacagca actgggccac caccaagttg
     2941 gccgtctggt cattgaccgg gcccaccagg aagatcactc gctccttgag cagacgggaa
     3001 cagatgtcat aggagcgctc gccccgaccc gactgctcaa tcaccatggg aaccatgccc
     3061 aggccctgga tatccattgc gcctggcgca ccgtatttca cattcatagc gtctccatta
     3121 aacatagtgg gaatgtaccc aaaaaccgtg ccagaaatgc aaatggggct tgacggcaag
     3181 accacaagcc ccgcccaagc cccatttatc gttcaagtca gtgacctgaa gccgcccaat
     3241 ctcagttctg acccatcaga tcgtcaaagg aaacagactt ctcgctgacc ttggctttgg
     3301 acagcacgaa gtcagtgaca ttgttttcaa tcacgatggc ttccacttcg gccatgcggc
     3361 ggttgtcgcc gtaataccag cgcaccacat cggcgggctt ctcgtagctg gcagccagct
     3421 catcaatgtg ggccttgatc tgctcgggcg tcgcttgcag gttgttggaa cgcaccaact
     3481 cagcgactac caagcccagg cgcacgcggc gctcagcctg ggggcggaag atatcgtcag
     3541 gaatcggtgc cttgtcagcg tccttgatgc cacgctgttt gaggtcggcg cgcgcgcctt
     3601 ccaccatgcg gtccagttca gcctgcacgc tggacttggg cagatccagt tcggacttgg
     3661 caatcagcgc gtccatcacg gcgttcttat tgcgacccag caggcggtac ttgacttcac
     3721 gctcgaggtt cttgcggata tcggcacgca ggccttcgac tgttgcttca gcaatgccca
     3781 gggacttggc caatgtgcca tccacttcag gcaggtgcgc tgcttcgatt ttcttgacgg
     3841 tcaccaggaa gtcggcagtc ttgccggcta cgtccttgcc gtggtaatca gcggggaatg
     3901 ccagctggaa agtcttgctt tcgccagact tcatgccgcg ggtggcgtct tcgaattcct
     3961 tgagcatctg gccatcgccc acgatgaact ggaagtcttc ggccttgccg ccagcgaacg
     4021 gttcgccgtc gatcttgccc tcaaagtcga cggtcacgcg gtcaccatcc tgggccgcag
     4081 cttccaaagc gcgctgcgca aaggtgcggc gctgtttgcg cagaatttcc accgtcttat
     4141 cgatggcagc atcggacact tcggtgttga tcttctcgac ttcagccgtg gtcaggtcac
     4201 cgatcttgac ttcgggaaac acttcaaaca cggcatcgaa agccatctgg ccttcgggcg
     4261 cggtttcatt ctcggagata cggggctgac cagccacacg cagcttggct tcgttggcag
     4321 cagcagcaaa cgcctcgccc accttgtcgt tcatgacttc atagtgcacg gaatagccat
     4381 agcgctgggc caccacgttc atgggcacct tgccaggacg gaaaccgtcc atcttcacgg
     4441 tacgggccag cttgcgcaga cgtgagtcca cttcggtctg aatcacgccc acaggcagtg
     4501 tcagcgtaat cttgcgttcc agcttttcaa gggtttctac agtaactgcc atgattcact
     4561 cctaaattat tggtgcgcgg ggggggactc gaacccccac actgttgcca gcgtcaggac
     4621 ctaaa
//
LOCUS       DAOSJM010000188         4570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16714_length_4570_cov_4.930454, whole genome shotgun sequence.
ACCESSION   DAOSJM010000188 DAOSJM010000000
VERSION     DAOSJM010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16714_length_4570_cov_4.930454"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /gene="pcaF"
                     /locus_tag="PKX82_07720"
     CDS             <1..1193
                     /gene="pcaF"
                     /locus_tag="PKX82_07720"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOF52180.1"
                     /translation="FICDAIRTPFGRYGGALSSIRTDDLGALPIKALMERNPGVDWAA
                     VTDVIYGCANQAGEDNRNVAHMSSLLAGLPMEVPGATINRLCGSGMDAVGTAARAIRA
                     GEATLMIAGGVESMSRAPFVMPKAESAFSRNNAVYDTTIGWRFINPLMKKLYGVDSMP
                     ETAENVATDFKIEREAQDQMALRSQLNAVRAQQGGFFEAEIVPVTIPQKKGDAVVVSQ
                     DEHPRATSLESLTKLKGVVRPDGTVTAGNASGVNDGACALLLANEAAARQYGLTPRAR
                     VVGMATAGLAPRIMGFGPAPATRKVLALTGLTLAQMDVIELNEAFAAQGLAVLRDLGL
                     ADDDARVNPNGGAIALGHPLGASGARLVTTAINQLHRTGGRYALCTMCIGVGQGIAIV
                     VERV"
     gene            complement(1210..2427)
                     /locus_tag="PKX82_07725"
     CDS             complement(1210..2427)
                     /locus_tag="PKX82_07725"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF52181.1"
                     /translation="MDGSDLPALEPSLIAEKVRSEMARVFVERLSDSVLSAPLGLAIT
                     GWLLQSVAGWTPALTWICGMAVVEVMIQSVGNRFQRALAQGNSTRGWVRAQCVASGLL
                     GLGWGSAVWFVWVDGQMLYYLAVLCALVGVSGICMVTMSPQPLATRLFSLGMLAPPLV
                     QLLWVDNPLAMQVGFGWLMMAAVQAWTARDLHRELARETKSALHNKDLLQQLSRTTTE
                     LHRASAQMEEKNTELAAALGQLEELVSHDQLTGAFSRRYIFEQLDRLAAISQRHGAPV
                     TAVMFDLDHFKAINDTYGHPTGDRALKEVVRAVADQLRDGDMLARVGGEEFLVVLPLT
                     GLGDALQLAERLRQTLALTTVAVEGGGVIFLPASFGLAELLPDEGHAEWFNRVDAALY
                     QAKEKGRNTLVAA"
     gene            complement(2441..3574)
                     /locus_tag="PKX82_07730"
     CDS             complement(2441..3574)
                     /locus_tag="PKX82_07730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF52182.1"
                     /translation="MADTTNLELDDEAIARHAAIERIRVFNERSITAARLAFVGIALM
                     AWVLATQAGWGRAIVWVAAMSLVEVVILLAGVGCRRALERKVHRHLWLRVQLVLAAVS
                     GMVWGSAVWFVWTPGQFIPFLATLTILIGVAGVSIITMSSYAVAAVAFFGGIYLVPLF
                     HEFVHPKQASHFLQAGLLVIMVVQIGYARSLGRMEKRELEQYVRNGALVVRLHDLVTH
                     DQLTGAFSRRYMLERLEQQVSLHERHGSPASLIMFDLDHFKAINDHYGHPAGDRALRE
                     VVRAVNTQLRDGDMLARVGGEEFLVLLPMTGMQDAHALAERLRQTLESTHIVEGSKKV
                     FLPASFGVAELLPKENFSEWLRRVDAALYQAKDQGRNSLFDAE"
     gene            complement(3543..>4570)
                     /locus_tag="PKX82_07735"
     CDS             complement(3543..>4570)
                     /locus_tag="PKX82_07735"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466223.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HOF52183.1"
                     /translation="SQCGALRQVRALGTTGSPLGEEAQRWGTDQFRKLRAQADAEWSA
                     AGPSQGARAPMGGSDPHAVGERGGDIWWCNISGGTDFAGAFIGGNRELPLVPGEMQCR
                     MLGAAVESWNEQGQPVVDEVGELVCTQPIPSMPLYLWGDENNQRYLSSYFDMYPAGHG
                     RKPGGGDDSAEAGPVWRHGDWLRIGSHGGCVIFGRSDATINRHGLRMGTSELYSAVEA
                     LPEVVDSMVVDLEYLGRESYMPLFVVLREGMVLDAALKDKIHNAIRTALSPRFVPNGT
                     FQVAEIPRTLSGKKQELPIKKLLLGQPLEKVVNKDAMANPGCLQWYVEFAQVRNHHGG
                     HHESGAG"
BASE COUNT          877 a         1585 c         1334 g          774 t
ORIGIN      
        1 cattcatctg tgacgccatc cgcaccccct ttggccgcta cggcggcgcc ctgtcttcca
       61 tccggaccga tgatctcggc gcccttccga tcaaggcgct gatggagcgc aaccccggcg
      121 tggactgggc tgctgtgacc gacgtgatct acggctgcgc caaccaggcc ggcgaagaca
      181 accgcaatgt ggcgcacatg tcatcgctgc tggcgggttt gccgatggaa gtgcccggtg
      241 ccaccatcaa ccgactgtgc ggctcgggta tggatgccgt tggcactgca gcccgcgcca
      301 tccgtgcagg agaagctaca ttaatgatag cgggcggcgt ggaaagcatg agccgcgcgc
      361 ccttcgtcat gcccaaagcc gaatcggcct tcagccgcaa caacgcggta tacgacacca
      421 ccatcggctg gcgctttatc aacccgctga tgaagaagct gtacggtgtg gactccatgc
      481 ccgagacggc agagaacgtg gctaccgact tcaagattga acgcgaagcc caggaccaga
      541 tggcactgcg ctcccagctc aatgcggtgc gcgcacagca aggtggcttc ttcgaagccg
      601 aaatcgtgcc ggtcaccatc ccccagaaaa agggggatgc ggttgtggtg tcacaggacg
      661 agcaccctcg cgccacctcc ctcgaatcac tgaccaagct caaaggtgtg gtgcgccccg
      721 acggcaccgt gaccgcgggc aatgcctcgg gcgtgaacga tggtgcgtgc gccctgctgc
      781 tggccaatga agccgctgcc cgccaatacg gcctgacgcc gcgcgcccgc gtggtcggca
      841 tggccaccgc cggtctggct ccgcgcatca tgggctttgg cccggcgccc gccacccgca
      901 aggtgctggc cctgaccggg ctgacgctgg cgcagatgga tgtgatcgaa ctcaacgaag
      961 cctttgccgc ccagggcctg gccgtgctgc gcgatctcgg cttggccgac gacgatgccc
     1021 gtgtgaaccc caatggcggt gcgattgcgc tgggccaccc gctgggggcc agcggcgcgc
     1081 gtctggtgac gaccgccatc aaccagctgc accgcaccgg tggccgctac gcgctgtgca
     1141 ccatgtgcat tggcgtgggc cagggcattg cgatcgtggt cgagcgggtg taaccccccc
     1201 ctgcgtcact caggccgcaa ccagcgtgtt gcggcctttt tctttggcct ggtacagcgc
     1261 cgcatcgacg cgattgaacc actccgcatg cccctcgtcg ggcagcaact cggccaaacc
     1321 aaacgacgca ggaagaaaga tgacacctcc gccttccacc gccaccgtgg tgagcgccag
     1381 cgtctggcgc agccgctcgg ccagctgcaa cgcatcaccc aagccggtca ggggcagtac
     1441 caccaggaac tcttctccgc ccacgcgggc cagcatgtcg ccgtcgcgca actggtccgc
     1501 aacagcgcgg accacttctt tcaaagcccg gtctccggtg gggtggccat aggtgtcgtt
     1561 gatggccttg aagtggtcca ggtcaaacat cacggcggtc accggagcgc catggcgctg
     1621 gctgatcgca gcaaggcgat caagctgttc aaagatatac cgacggctga acgcaccggt
     1681 cagctggtca tggctcacca gctcttccag ctggccaagg gcagccgcca gttcggtgtt
     1741 cttctcttcc atttgtgcac tcgcacggtg aagttcagta gtggttctgg agagttgttg
     1801 caacagatcc ttgttatgca gggcgctttt tgtctcgcgc gcaagttccc ggtgcaggtc
     1861 ccgtgccgtc caggcctgta ccgcggccat catcagccag ccaaagccca cctgcatcgc
     1921 aagcgggtta tccacccaga gaagttgcac gagcgggggc gccagcatgc ccagcgagaa
     1981 cagccgggtc gccaggggct ggggtgacat ggtcaccatg cagatacccg aaacgcccac
     2041 cagcgcacag aggaccgcca ggtagtacag catctgtccg tctacccaga caaaccacac
     2101 ggcacttccc caacccagtc ccagcaagcc ggaagcaacg cattgcgccc taacccagcc
     2161 tcgggtgctg tttccctgcg caagcgcgcg ctgaaaccgg ttgcccaccg actggatcat
     2221 gacctccacc accgccatgc cgcaaatcca cgtcagggca ggtgtccagc ctgctacgga
     2281 ctggaggagc caccccgtga tggcaagccc cagcggcgcc gaaagcacac tgtcggacag
     2341 gcgctcgaca aacacacgcg ccatctccga tcgcaccttt tcggcaatca gcgatggttc
     2401 cagcgctggc agatccgacc cgtccatggt tctatgcccc ctactccgca tcaaacagcg
     2461 agttgcgccc ctggtccttg gcctggtaca gcgcagcatc cacgcggcgc agccattcgg
     2521 agaagttttc tttcggcagc aactcggcca cgccaaagga cgccggaaga aaaacttttt
     2581 tggagccctc cacaatgtgg gtggactcca gcgtctggcg cagccgctcg gccagtgcat
     2641 gcgcgtcctg catgccagtc atcggcagca gcaccagaaa ctcttctccg cccacacgcg
     2701 ccagcatgtc gccatcgcgc agctgtgtgt tgactgcacg caccacttcc cgcagcgccc
     2761 ggtccccggc cgggtggccg tagtggtcgt tgatggcctt gaaatggtcg aggtcgaaca
     2821 tgatgagcga cgccggtgaa ccgtgccgct cgtggagcga tacctgctgc tccagccgct
     2881 ccagcatgta gcggcggctg aacgcaccgg tcagctggtc gtgggtgacg aggtcgtgca
     2941 gacggacgac caaagccccg ttgcggacgt attgctccag ctcccgcttc tccatcctgc
     3001 cgagactgcg tgcatagccg atctgcacca ccataatcac cagcagaccc gcctgcagaa
     3061 aatgggaggc ctgtttcgga tggacaaatt catggaacag cggcaccaga tagatgccac
     3121 cgaaaaaggc aaccgccgcc accgcgtagg acgacatggt gatgatgctg acaccggcca
     3181 ccccaatcag gatggtcagc gtcgccagga aaggaatgaa ctgcccggga gtccagacaa
     3241 accacacggc cgacccccag accatcccgc tgaccgcggc cagcaccagc tgcacgcgca
     3301 gccagagatg gcgatgcact ttgcgctcca gagcgcgccg gcaccccaca ccggccagca
     3361 gaatcaccac ctccaccagg ctcatggccg caacccagac gatggcccgt ccccaccccg
     3421 cctgcgtcgc caggacccag gccatcagcg caatccctac aaacgccagg cgcgccgcag
     3481 tgatggagcg ctcattgaaa acgcggatgc gctcaatcgc cgcatggcgc gctatcgcct
     3541 cgtcatccag ctccagattc gtggtgtccg ccatggtggt tcctgacctg cgcaaactct
     3601 acataccatt gtaggcaacc agggttggcc atggcatcct tattgaccac cttttcgagc
     3661 ggctgcccca atagcagctt cttgatgggc aattcctgct ttttccccga caaagtgcgc
     3721 ggaatttccg ccacctgaaa ggtgccgttg ggaacgaagc gcggcgacag cgcggtgcgg
     3781 atggcgttgt ggatcttgtc cttcagtgca gcatcgagca ccatgccctc gcgcagcacg
     3841 acgaacagcg gcatatagct ttctcgcccc aggtattcca gatccaccac catggagtcg
     3901 acgacctccg gcaaggcctc taccgcgctg tacaactcgc tggtgcccat gcgcaggccg
     3961 tggcggttga tggtggcatc gctgcggcca aaaatgacac agccgccgtg cgatccgatg
     4021 cgcagccagt cgccgtgccg ccacaccggg ccggcctctg ctgaatcatc tccgccgccg
     4081 ggtttgcggc cgtggcctgc ggggtacatg tcaaagtagc tcgacagata ccgctggttg
     4141 ttctcgtccc cccacaggta cagcggcatg ctcggaatcg gctgggtgca gaccagttcg
     4201 cccacctcgt ccaccaccgg ttggccctgc tcattccagg actcgaccgc tgcgcccagc
     4261 atgcggcact gcatctcgcc cggcaccagt ggcagctcgc ggttaccacc aataaaagca
     4321 ccagcaaagt cggtgccacc ggagatattg caccaccaaa tgtcgccccc acgctccccc
     4381 actgcgtgtg ggtcgctgcc ccccatgggg gccctcgcgc cttgggacgg cccggcggcg
     4441 ctccattcgg catcagcctg cgctctgagt tttcggaact ggtctgtgcc ccaacgctgg
     4501 gcctcttcac ccaacggtga accggtggtg cccagcgcgc gcacctgccg caacgcccca
     4561 cactgggaca
//
LOCUS       DAOSJM010000189         4546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16831_length_4546_cov_3.961924, whole genome shotgun sequence.
ACCESSION   DAOSJM010000189 DAOSJM010000000
VERSION     DAOSJM010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16831_length_4546_cov_3.961924"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="PKX82_07740"
     CDS             <1..958
                     /locus_tag="PKX82_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOF52184.1"
                     /translation="KPMVPASGPKFGFVNVLYYFGGMVAISAMSLFMTLGFQSMGAGG
                     LLAIGVAYLFACLKVADHFKARGLAVPAGILATLAVVLVPLVVWCVQSLLGLWPPGGS
                     SNFSDYHTFINWRWTTLEFATLAAGVVMLWRYRLPFMVMPIAVTVWYMSMDIANALMQ
                     NHGFDWQFTRDVSLVFGIGTIALAMWVDVRSRRSATPEWRQDFAFWLYLFGAIMFWGS
                     LSMRDSSSEFGKFMYALLNVALVLGGAAIGRRVFTVLGALGVAGYLGYLSHRVFQNSM
                     LFPFVLTLIGLGVVWLGVWWQRHEEAINARFARFVPEGLRPR"
     gene            complement(966..1712)
                     /locus_tag="PKX82_07745"
     CDS             complement(966..1712)
                     /locus_tag="PKX82_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52185.1"
                     /translation="MPFATRTRGLATALTAWVLSGLGPALAEEPIVLHYYTRPPFMER
                     DAGNHILGLTATPAADAFNKAGISFVWKETPAKRQLAVIASNTGRDCGLGWYRTAERD
                     AIGKFSVPIRSDRATVGVARRDFQPGSTRLADIVSNPSVRIFQKDGLTYGQEIPGILA
                     RAKSNVQVLTRDQVTLAQMVGLQRADFMFALPEEAELLVQSPALARYGLKTITFSDIK
                     GGDTRHILCSKRVDEDTLARLNQALVSVRP"
     gene            complement(1718..2311)
                     /locus_tag="PKX82_07750"
     CDS             complement(1718..2311)
                     /locus_tag="PKX82_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52186.1"
                     /translation="MSTIRYTTVPNLLPENKMRLYPTKVINIIGGPGCDKSLISSAII
                     LYLHLQNKTVETIPDYAKSLVWQQNYEVLKNQYFIAQRQFEMLSLLDGQVQFLITECS
                     LPQVMYYNENYAENICDIAKTRAQILEWYGQYDNVNIFVERGEKKYVHTGRFQDEEQA
                     RVIDRGLRDVMEREKLPYTVLQPNVQEINAFTATLME"
     gene            2429..3535
                     /locus_tag="PKX82_07755"
     CDS             2429..3535
                     /locus_tag="PKX82_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF52187.1"
                     /translation="MKLWTSVSAALLLALLGTAAHAQILIGQSAGFSGPVAAGVKETT
                     DGARLYLNAVNAKGGINGQKIELVSMDDKFDPKLAAENARVLIEEKEVVAMFLTRGTP
                     HTEAMLPLLDKHGVSLVGPSTGAMVLHQPVKKHVFNVRATYQREAEKAIAHLATIGIT
                     RIAVVHSDDSFGADGVTGAQRGLDAAKQAAVAVEKYDRAKPDFSKIAPKIAKTDAQAV
                     MVIGSGAAVVDCIKAVKAAGGTPQFVTLSNNASGGFVKSLGDLARGIIVTQVLPQSMG
                     YAVVKEINELARAQSITDVSPAMLEGFVSAKVLVEALRRAGPKLNRDSIQSALEGLRK
                     FDVGGLEVSYSRDDHTGLDFADLSIISDTGKFRR"
     gene            complement(3562..4377)
                     /locus_tag="PKX82_07760"
     CDS             complement(3562..4377)
                     /locus_tag="PKX82_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOF52188.1"
                     /translation="MSQTSATALIAEDEPLLAQALQAELARAWPVLQIVATVGDGASA
                     VAQALALQPDVLFFDIRMPGLSGLEAAAELADRWDTAARPFPALVFVTAYDQYAVQAF
                     EAQAVDYVLKPVQADRLAKTVSKLQLALARPAQAAINMEANLEKAMAQLRALMAAPAS
                     AMVAPLTVIQASVGNAIRMVPIDEVLYFEAADKYVRVVTASHEYLIRTPLKDLLPQLD
                     STTFWQVHRGTLVHAAAIDAVHRDDAGKLHLTLRGRTEKLPVSRLYAHLFKAM"
BASE COUNT          773 a         1291 c         1543 g          939 t
ORIGIN      
        1 caagcccatg gtgcccgcca gcgggccgaa gtttggcttt gtgaacgtgc tgtattactt
       61 tggcggcatg gtggccatca gcgccatgag cctgttcatg acgctggggt tccagtccat
      121 gggcgcgggc gggctgctgg ccattggggt ggcctacctg tttgcctgcc tgaaggtggc
      181 agaccacttc aaggcgcgtg gcctggcggt gcctgcgggc attctggcca cgctggccgt
      241 ggtgctggtg ccgctggtgg tgtggtgcgt gcagagcctg ctgggcctgt ggccgcccgg
      301 gggcagcagc aatttttcgg actaccacac gtttatcaac tggcgctgga ccacgctgga
      361 atttgccacg ctggccgccg gtgtggtgat gctgtggcgc taccggctgc cgttcatggt
      421 gatgccgatt gcggtgacgg tctggtacat gagcatggac attgccaatg cgctcatgca
      481 gaaccacggg tttgactggc agttcacgcg cgatgtgtcg ctggtgtttg gcatcggcac
      541 catcgcattg gcgatgtggg tggatgtgcg cagccgccgc tctgccacgc cagagtggcg
      601 gcaggacttt gccttctggc tctacctgtt tggcgccatc atgttctggg gttccctgag
      661 catgcgggac tccagcagcg agtttggcaa gttcatgtat gcgctgctca acgtggcgct
      721 ggtgctgggc ggtgcggcca tcgggcggcg ggtgtttacc gtgctggggg cgctgggcgt
      781 ggcgggctac ctggggtact tgtcgcaccg ggtgtttcag aacagcatgc tgttcccgtt
      841 tgtgctcacg ctgattggcc tgggggtggt atggctgggc gtctggtggc agcgccatga
      901 ggaagccatc aacgcgcgct ttgcccgctt tgtgcccgaa ggcctgaggc cgcgttaagg
      961 tcgcgttaag gtcgtactga gaccagtgcc tggttgagcc gggccagggt gtcttcatcg
     1021 acacgcttgc tgcacaggat gtggcgggtg tcgccgccct tgatgtcgct gaaggtgatg
     1081 gtcttgaggc catagcgggc cagcgcgggg ctttggacca gcaactcggc ttcttcaggc
     1141 agggcaaaca tgaagtcggc acgttgcagc cccaccattt gcgccagcgt gacctggtca
     1201 cgggtgagga cctgcacgtt gcttttggcg cgggccagaa tgccggggat ttcctggccg
     1261 taggtcaggc cgtccttttg aaaaatccgc accgaggggt tcgagacaat atcggccagc
     1321 cgcgtgctgc cgggctgaaa gtcacgccgt gccacgccca cggtggcccg gtcggaccgg
     1381 atgggtacgc tgaatttgcc gatggcgtcg cgttctgcgg tgcggtacca gcccaggccg
     1441 cagtcgcgcc cggtgttgct ggcgatgacg gcgagttgcc gcttggcagg ggtttctttc
     1501 cacacgaagg aaatgccagc cttgttgaag gcgtcagcgg cgggggtggc ggtcaggccc
     1561 aggatgtggt tgccggcgtc cctttccatg aacggcggac gcgtgtagta gtgcagcacg
     1621 atgggctctt cggccagtgc cgggcccaag ccggacagaa cccaggcagt aagtgcggtg
     1681 gctagcccgc gtgttcgtgt ggcaaagggc atgccaccta ttccatcagt gtggcggtga
     1741 aagcgttgat ttcctgcaca ttgggctgca gcacggtgta gggcagcttt tcacgttcca
     1801 tcacatcacg cagtccgcgg tcgatcacgc gggcctgttc ctcatcctgg aagcggcccg
     1861 tgtgcacata ctttttctcg ccacgctcga caaagatgtt gacgttgtcg tactgcccgt
     1921 accactccag aatctgggcg cgggtcttgg caatgtcgca gatgttctcg gcgtagtttt
     1981 cgttgtagta catcacctgg ggcagggagc actcggtaat caggaactgc acctggccat
     2041 cgagcaggct gagcatttca aactggcgct gggcgatgaa gtactggttc ttcagcacct
     2101 catagttttg ctgccacacc agtgacttgg cgtagtcggg gatggtctcc acggtcttgt
     2161 tctgcaggtg caggtacagg atgatggccg acgagatgag ggacttgtcg cagcctggcc
     2221 cgccaatgat gttgatgacc ttggtggggt acagccgcat cttgttttcc ggcagcaggt
     2281 tgggaactgt tgtgtaacga attgttgaca tgacggggcc ttgcgaaatt cttgcgattt
     2341 tgcatgctag cctgcccccc agtcttgcgc aagtaaaccg gtgcagaccg gcatttcgca
     2401 ccttatacga attcatggag acacatcaat gaaactctgg acctctgttt cggcggcgtt
     2461 gctgctggcc cttctgggca ctgcagccca cgcacagata ctgattgggc agtccgcggg
     2521 ctttagcggg ccggtggcgg ctggcgtgaa ggagaccacc gatggcgcca ggctctacct
     2581 caatgcggtc aatgccaagg ggggcatcaa cgggcaaaag atcgagctgg tctcgatgga
     2641 tgacaagttc gatcccaaac tggcggcaga aaacgcgcgc gtgctgatcg aggagaagga
     2701 ggtggtggcg atgttcctca cccgcggcac gccccatacc gaggccatgc tgcccctgct
     2761 ggacaaacac ggtgtctccc tggtggggcc ctccactggc gccatggtgc tgcaccagcc
     2821 ggtgaaaaaa catgtgttca atgtgcgcgc cacctaccag cgcgaagcag aaaaggcgat
     2881 tgcccacctg gccaccatcg gcatcacccg gattgcggtg gtgcattcgg atgacagctt
     2941 tggcgccgac ggggtgaccg gtgcgcagag gggactggac gctgcaaagc aggccgctgt
     3001 ggcggtggag aaatatgacc gggccaagcc agacttttcc aaaatcgcgc ccaagattgc
     3061 caagaccgat gcccaggcgg tgatggtcat tggctcgggc gccgcggtgg tggattgcat
     3121 caaggccgtc aaggccgcgg gcggaacccc ccagtttgtg acactgtcca acaacgcgtc
     3181 gggtggattc gtcaagagtc tgggggatct ggcgcgcggc atcatcgtga cgcaggtgtt
     3241 gccgcagtcc atgggctatg ccgtggtgaa ggaaatcaat gagctggccc gggcgcaaag
     3301 cattaccgac gtgagcccgg ccatgctgga agggtttgta tcggccaagg tgctggtgga
     3361 agccttgcgc cgcgccggac ccaaactcaa ccgggacagc attcagtcgg cgctggaggg
     3421 gttgcgcaaa tttgacgttg gcggcctgga ggtcagctac agccgcgatg accacaccgg
     3481 cctggacttt gccgatcttt ccatcatcag cgacacgggc aagttccggc gctaggcgca
     3541 cccgttgccg ccgtggcgga tctacatcgc cttgaacagg tgggcgtaga ggcggctgac
     3601 cggcagcttt tcggtgcggc cgcgcagggt gaggtgcagt ttcccggcat cgtcgcggtg
     3661 cacggcgtcg atggcggcgg cgtgcaccag cgtgccgcgg tggacctgcc agaacgtggt
     3721 ggagtccagt tgtggcaaga ggtctttgag cggagtgcgt atcaggtatt catggctggc
     3781 cgtgaccacg cgcacgtatt tgtcggccgc ctcaaagtag agcacctcgt cgatgggcac
     3841 catgcggatg gcattgccca cactggcctg gatcacggtc aacggtgcca ccatcgcact
     3901 ggcaggtgcg gccatcagtg cacgcaactg cgccatcgcc ttctccagat ttgcttccat
     3961 attgatagcg gcctgcgcag gtctggcgag ggctagctgc agttttgaca cggtttttgc
     4021 cagccggtcg gcctgcaccg gcttgagcac atagtccacc gcctgggctt caaaggcctg
     4081 caccgcgtac tggtcgtagg cggtcacaaa caccagcgcg ggaaaagggc gggccgccgt
     4141 gtcccaccgg tcggccagct ctgcggcggc ctccagtccg cttagtccgg gcatgcggat
     4201 gtcaaaaaac agcacatcgg gttgcagtgc cagggcttgc gccacggccg aggcaccgtc
     4261 gcccacggtg gccacgatct gcagcacagg ccaggcccgc gccagttcgg cctgcagggc
     4321 ctgggcgagc aggggttcgt cttcggcaat cagggcggtg gcggaggtct ggctcatgtt
     4381 tttaagggaa ttgtgatgct agcccgcgta ccgcctgcgg aaccagctat caacttaata
     4441 gcagattcag tgccgtacag ggtggccagg cgctcgcgta cctggcgcag gccaaagcct
     4501 ttgccttcaa gcggcgtggc ccgggagtcc aggcccttgc cggtgt
//
LOCUS       DAOSJM010000190         4517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16959_length_4517_cov_4.205513, whole genome shotgun sequence.
ACCESSION   DAOSJM010000190 DAOSJM010000000
VERSION     DAOSJM010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16959_length_4517_cov_4.205513"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PKX82_07765"
     CDS             complement(<1..808)
                     /locus_tag="PKX82_07765"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HOF52189.1"
                     /translation="MEIFDYDNILLLPRKCRVESRSECDAGVELGGRKFRIPVVPANM
                     KTVVDEATCVWLAQNGYFYVMHRFDLDNVQFVKDMKAKGLYASISLGVKKPDYATVDT
                     LAALGLTPEYVTIDIAHGHADSVKDMIVYLKGKMPSTFIIAGNVATPEAVIDLENWGA
                     DATKVGVGPGKVCITKLKTGFGTGGWQLSALKWCARVATKPIIADGGIRSHGDIAKSI
                     RFGASMVMIGSLFAGHEESPGKTVEVDGALFKEYYGSASDFNKGEYKHVEG"
     gene            1204..3606
                     /locus_tag="PKX82_07770"
     CDS             1204..3606
                     /locus_tag="PKX82_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOF52190.1"
                     /translation="MSLFSATDHASQSPLLATLNPEQSAAVTLPAEHALILAGAGSGK
                     TRVLTTRIAWLLQNGYVSPGGILAVTFTNKAAKEMMTRLSAMLPVNVRGMWIGTFHGL
                     CNRFLRAHYKPAGLPQAFQILDTQDQLSAIKRLCKQFKIDDERFPPKQLMWFIAGCKE
                     EGLRPKDVVASDPDTRKKVEVYQLYEDQCQREGVVDFGELMLRSYELLRDNDPIREHY
                     QRRFRHILIDEFQDTNKLQYAWIKMIAGLGQGASPDGAGGAVLAVGDDDQSIYAFRGA
                     RVGNMADFVREFRVQHQIKLEQNYRSYSNILDSANALISHNSKRLGKNLRTDQGPGEP
                     VRVYEAPSDFAEAQWMVDEMKQLVRDDVERKEIAVLYRSNAQSRVIETALFNAGMPYR
                     VYGGLRFFERAEIKHALAYLRLLENPNDDTSFMRVVNFPARGIGARSIEQLQDVARAT
                     GCSLHDAVSALSGRAGTLIASFVAKLDVLREQTVGMNLKNIIQLMLDHSGLLDHYRND
                     REGEDRLENLDELVNAAESFVMQEGFGREAPGMVPLVSAPEALTQSPASQGLTGDALT
                     AANAEFGDTGETLSPLQAFLTHAALEAGDNMAAAGQDAIQLMTVHSSKGLEFDCVFIT
                     GMEEGLFPHENSMSDHEGLEEERRLMYVAITRARKRLYLSHSQTRMLHGQTRYNLKSR
                     FFDELPEVALKYLTPKQPPAGAARSWGATFAPNSGAGHAYSTGARGQFDSYSAVQKEV
                     PRKEEPTGLRKGQKVFHAKFGEGTVLTLEGAGDDARAQINFPRHGTKWLALSVAKLTP
                     VP"
     gene            3644..>4517
                     /locus_tag="PKX82_07775"
     CDS             3644..>4517
                     /locus_tag="PKX82_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52191.1"
                     /translation="MQGVPTQNARVVDVGVWEPSSVYLKLLVGLVLGAITAFLISLYL
                     FAPGQRVQALSAVALAVLMLVAWIQVRRGDVRCALRVVTHGVWLVATGTVIASGSLRT
                     PIIYAYPVIILAMGLLIGAGIARRTTLATVVVIFLLGVGETTGLTAASVTPAPAMFGV
                     IQITVCLLALLLARAVVKAYQARLGELKILGQNLTLRTQHLEESRLQLQQAQAVARVG
                     SWVMDLSTGEFTLSAETQRIFGMTQRPGGDTASMLECTFHEDRAQLERALVDARRGVG
                     FDFEQRIVVADAVHW"
BASE COUNT          862 a         1341 c         1426 g          888 t
ORIGIN      
        1 tgccttccac gtgcttgtac tcgcccttgt tgaagtcgct ggccgagccg tagtactcct
       61 tgaacagcgc gccgtccact tccaccgttt tgcccggtga ttcctcatgg ccggcaaaca
      121 gcgagccgat catcaccatg ctggcgccaa agcggatgct cttggcaatg tcgccatggc
      181 tgcggatgcc gccgtcggca atgatgggct tggtggccac gcgcgcgcac cacttgagcg
      241 ccgagagctg ccaaccgccg gtgccaaagc cggtcttgag cttggtgatg caaacttttc
      301 ccgggcccac gccgactttg gtggcgtctg cgccccagtt ttccaggtcg atcaccgcct
      361 cgggcgtggc cacgttgccg gcaatgatga aggtcgatgg catcttgccc ttgaggtaga
      421 cgatcatgtc cttcacgctg tcggcgtggc cgtgcgcgat atcgatggtc acgtactcgg
      481 gtgtcaggcc cagtgctgcc agcgtgtcca ccgtcgcgta gtcgggcttt ttgacgccca
      541 gggagatcga cgcatacagg cccttggcct tcatgtcctt gacgaactgc acgttgtcca
      601 ggtcaaagcg gtgcatcacg tagaagtagc cgttctgtgc cagccagacg caggtggctt
      661 catccaccac ggttttcatg ttggcgggca ccaccgggat gcggaatttg cggccgccga
      721 gctccacgcc cgcgtcgcat tcggatcggc tttccacgcg gcatttgcgt ggcagcagaa
      781 ggatgttgtc gtagtcaaaa atttccatgg cccaagctcc tgttcctgtt tcaagaaagt
      841 agtttcaaac aaacgcgggg cgcttggact ggtaccggcc ataaaaaaac cgggtgccaa
      901 ttgcttgggc ccggtgcttg attgtataca ccttgcggat tgttgtggtg gatcagggtg
      961 gcagtgcgct ttgctgcgtg tcccccgccc gctttgcggg ctcctccttt acctacgcaa
     1021 aacgcactgc caccctgatc cgcttacgta attgggttgc gtgcctacgc aaaaagcgcc
     1081 gataacgaca gagagtttgg gctgggggtt ctgcgtaggt aaaggaggag cccgaagggc
     1141 gggggacacg gagcagagca cgcagcccga acgctccgcg acgatgcttt ctacaatggc
     1201 tccatgtctc ttttttccgc caccgaccac gcgtcacagt ccccgctgct tgccaccctc
     1261 aaccccgagc aaagtgctgc cgttacgctt cccgcagagc acgcgctgat cctggctggc
     1321 gcgggctcgg gcaagacgcg ggtgctgacc acccgcatcg cctggctgct gcaaaacggc
     1381 tatgtttcgc ccggtggcat tctggcggtg acgtttacca acaaggccgc caaggagatg
     1441 atgacgcggc tcagcgccat gctgccggtg aatgtgcgcg gcatgtggat tggcaccttc
     1501 cacggcctgt gcaaccgctt cctgcgggca cactacaagc ctgcagggtt gccccaagct
     1561 tttcaaattc tggataccca ggatcaactc tccgccatca agcggctgtg caagcagttc
     1621 aaaatcgacg acgagcgctt tccgcccaag caactgatgt ggttcattgc gggttgcaaa
     1681 gaggaggggc tgcgtcccaa ggatgtggtc gccagcgacc cggacacccg caagaaggtc
     1741 gaggtgtacc agctctacga agaccagtgc cagcgcgaag gcgtggtgga ctttggtgaa
     1801 ctgatgctgc gcagctatga gctgctgcgc gacaacgacc ccattcgcga gcactaccag
     1861 cgccggtttc gccatattct gattgacgag tttcaggaca ccaacaagct gcagtacgcc
     1921 tggatcaaga tgatcgcggg actggggcag ggcgcgagcc ccgatggtgc tggcggtgcc
     1981 gtgctggcgg tgggcgacga tgaccagagc atctatgcct tccgtggtgc gcgtgtgggc
     2041 aacatggccg attttgtgcg cgagttccgg gtgcagcacc agatcaagct ggagcagaac
     2101 taccgcagtt acagcaatat cctggacagc gccaacgcgc ttatcagcca caacagcaag
     2161 cgcctgggca agaatctgcg caccgaccag ggccccggcg agcctgtgcg cgtgtacgag
     2221 gcacccagtg actttgccga agcgcagtgg atggtcgacg agatgaagca actggtgcgc
     2281 gacgatgtcg agcgcaagga aattgccgtg ctttaccgct ccaacgcaca gagccgtgtg
     2341 attgagactg cgctgttcaa tgcgggcatg ccgtaccggg tgtacggcgg cctgcgcttc
     2401 ttcgagcggg ccgagatcaa gcacgcgctg gcgtacctgc gcctgctgga gaaccccaac
     2461 gacgacacca gcttcatgcg ggtagtcaac tttccggcgc gtggaattgg cgcacgcagc
     2521 atcgagcagc tgcaggacgt ggcccgtgcc acgggttgtt cgctgcacga tgcggtgagc
     2581 gcgctgtcgg gccgggccgg cacgctgatc gccagctttg tagccaagct cgatgtgctg
     2641 cgcgagcaga ccgtgggcat gaacctcaag aacatcatcc agttgatgct ggaccacagc
     2701 ggtctgctgg accactaccg caatgaccgt gagggcgagg accgcctcga gaatctggac
     2761 gaactggtca acgctgccga gagctttgtg atgcaggaag gcttcgggcg cgaggcaccg
     2821 ggcatggtgc cgctggtgag cgcccccgag gcgctcaccc agagcccagc cagccagggg
     2881 ctgacaggag atgccctgac ggcagccaat gccgagtttg gagatacggg ggagaccctc
     2941 tcgcccctgc aggccttctt gacccacgca gcgctggaag ccggggacaa catggccgcg
     3001 gccggtcagg acgccatcca gctcatgaca gtgcactcct ccaagggact ggagttcgac
     3061 tgcgtgttca ttaccggcat ggaggagggc ctgttcccgc atgagaactc catgagcgac
     3121 catgaaggcc tggaggaaga gcgccgcctg atgtatgtgg ccatcacccg cgcgcgcaag
     3181 cggctgtacc tgagccattc gcaaacccgc atgctgcatg gccagacgcg ctacaacctc
     3241 aagagccgat tctttgacga attgcccgag gtcgcgctga agtacctcac acccaaacag
     3301 ccgcccgcgg gagctgcgcg cagctggggt gctacatttg ctcctaattc aggagctggt
     3361 cacgcatatt ccacgggggc tagaggccaa tttgattcat attctgcggt ccagaaagaa
     3421 gtgccccgaa aagaggagcc cacgggcttg cgcaagggcc agaaggtatt ccatgccaaa
     3481 ttcggcgagg gcaccgtgct cacgctcgaa ggcgcaggcg acgatgcacg tgcgcagatc
     3541 aacttcccgc gccacgggac caagtggctg gcgctgagcg tggccaagct cacgccagtg
     3601 ccataaatac acaaaaaatg agagacacag tattgtgaaa gacatgcagg gagttcctac
     3661 ccagaatgcc cgggtggttg acgtcggtgt ttgggagccg tcttcggtct atctgaagct
     3721 gttggtgggt ctggttctgg gcgccatcac cgctttcctg atcagtctgt acctgtttgc
     3781 cccagggcag cgtgtacagg cattgagtgc ggttgcgctg gccgtgttga tgctcgttgc
     3841 gtggatacag gtgcgacgcg gtgacgtccg gtgtgccctg cgggtagtga cccatggtgt
     3901 gtggctggtt gccaccggta ccgtcatcgc cagcggcagt ttgcgcaccc ccatcatcta
     3961 tgcctatcct gtcatcattc tggcgatggg gttgctgatt ggtgctggca ttgcccggcg
     4021 caccacactg gcgacggtgg tagtgatctt tctgctgggg gtaggagaaa ccaccggact
     4081 gactgcagca agtgtgaccc cggcgcccgc catgttcggg gtgatccaga ttactgtctg
     4141 tctgctggcc ctgttgctgg cccgggcggt cgtgaaggcg taccaggcac ggctcgggga
     4201 gctgaaaatc cttggtcaga acctgaccct gcgtacccag cacctggaag aaagccggct
     4261 gcagttgcaa caggcccagg cagttgctcg ggtcggcagc tgggtcatgg atctgtccac
     4321 cggcgagttc acgctgtctg ccgagacgca acgtattttt ggcatgaccc agcggccagg
     4381 gggcgacacc gcgtccatgc tcgaatgcac attccatgag gaccgggcgc aattggagcg
     4441 cgcacttgtc gacgcgcgca gaggtgtcgg tttcgatttt gagcagcgca ttgtggtggc
     4501 agatgccgtg cactggg
//
LOCUS       DAOSJM010000191         4514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_16975_length_4514_cov_4.385064, whole genome shotgun sequence.
ACCESSION   DAOSJM010000191 DAOSJM010000000
VERSION     DAOSJM010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16975_length_4514_cov_4.385064"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PKX82_07780"
     CDS             <1..570
                     /locus_tag="PKX82_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HOF52192.1"
                     /translation="PYAFQGAGHVPAQTPQPYRPENVGNDASARPFERSNAMPLDSGL
                     QSGSMIGSALMGSQSWGIPAGFDSEGFLKAAKTNFVTLQAAWDRSDIASLRAMMTDSM
                     LGEIQAQLTEREKLTGSTPNFTEVVMIEARLLGIEEMANEYMASVEFSGMIREEPSAG
                     PNPFREVWNMTKARNGGSGWLVAGVQALQ"
     gene            618..1145
                     /locus_tag="PKX82_07785"
     CDS             618..1145
                     /locus_tag="PKX82_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52193.1"
                     /translation="MATQSPFSLLEGLFQSFNLPMTPPAWAVDEAHRKTVLLLNHVLQ
                     QEPQAMERLARQKGRVVLSQWREFSFKVLVTPAGLLDLASADAASDLTLVVTEQSPLA
                     IAQSVMQGDKPAVRIEGDVQLAAEINWLADHVRWDLEEDLARVLGDAPAHMVVQAGRT
                     LAQGLGQFVGKRTPA"
     gene            1142..2707
                     /gene="ubiB"
                     /locus_tag="PKX82_07790"
     CDS             1142..2707
                     /gene="ubiB"
                     /locus_tag="PKX82_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HOF52194.1"
                     /translation="MTRIYRGIFIVWVALRYGLDELVLTSFQKPWLRLVARIVSVGRN
                     LEAPRGQRLREALERLGPIFVKFGQVLSTRRDLLPLDIADELAKLQDRVPPFSSAVAV
                     ATIERELRKPLNQIFVSFEQIPVASASIAQVHFAVLRDRKGQEREVAVKVLRPGMAKV
                     IDKDLSLMRMMAKWLERLSADGKRLKPREVVAEFDKYLHDELDLVREASSAAQLRRNM
                     DGLNLVLIPEMLWDYCSTEVLVMERMHGVPISQVDRLRAAGVDIPKLARDGVTIFFTQ
                     VFRDGFFHADMHPGNIQVSLAPETFGRYISLDFGIVGTLTEVDKEYLAQNFVAFFRRD
                     YKRVAELHIESGWVPSSTRVDELEAAVRAVCEPYFDRPLKEISLGMVLMRLFQASRRF
                     HVEIQPQLVLLQKTLLNIEGLGRELDPELDLWATAKPFLEQWMLQQVGPQRLIEELKD
                     HAPRYARLLPELPGLIQAYLRRNGSDNAVLVQELLLEQRRTNRLLQTLVSAVIGFALG
                     MVVMQIVIRVGVF"
     gene            2760..3047
                     /locus_tag="PKX82_07795"
     CDS             2760..3047
                     /locus_tag="PKX82_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOF52195.1"
                     /translation="MPIYAYKCESCGFAKDVLQKISDAPLEQCPSCSQPTFKKQLTAA
                     GFQLKGSGWYATDFKGGATSTSATATATEGAAASPKADAAPSTPAAPAAST"
     gene            3062..3703
                     /locus_tag="PKX82_07800"
     CDS             3062..3703
                     /locus_tag="PKX82_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOF52196.1"
                     /translation="MKKYLLTGLMVWLPLAITIWVLLWLVGMLDAVFGGFLSVLAAVT
                     PAAFAPVIERLHHIPGLGVLLVFSVMLVTGALVANVAGRWWIKQWDRLFTNIPIVKSI
                     YNSVKKVSDTLFSSNGNAFRTAMLIQYPRAGSWTIAFQTGVPSGEVSSHLGSDFVSVY
                     VPTTPNPTSGFFLMVPRADVIELDMSVDQALTYVISMGSVAPSQHLPKAVPPK"
     gene            3750..>4514
                     /gene="aspS"
                     /locus_tag="PKX82_07805"
     CDS             3750..>4514
                     /gene="aspS"
                     /locus_tag="PKX82_07805"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOF52197.1"
                     /translation="MAMRSHYCGLVTEALMGETVSLCGWVNRRRDHGGVIFVDLRDRE
                     GYVQVVCDPDRADMFAVAEDVRNEFCVQVKGVVRARPEGTANDNLKSGKIEVLCHELI
                     VLNASVTPPFQLDDDNLSETTRLTHRVLDLRRPYMQNNLMLRYRVSMEVRKFLDANGF
                     VDIETPMLTKSTPEGARDYLVPSRVHDGHFFALPQSPQLFKQLLMVAGFDRYYQITKC
                     FRDEDLRADRQPEFTQIDIETSFMEEQDIRDMFQGMI"
BASE COUNT          861 a         1356 c         1368 g          929 t
ORIGIN      
        1 ccctatgcgt ttcagggcgc gggccatgtg cctgcccaga ctccacaacc ttaccgcccg
       61 gaaaatgtgg ggaacgatgc ctctgcgcgt cctttcgagc gcagcaatgc catgccgttg
      121 gacagtggtc tccagtcggg ttccatgatc ggttcggccc tgatgggatc gcaaagctgg
      181 ggcattcctg cgggatttga cagcgaaggt ttcctgaaag ctgcaaagac caatttcgtg
      241 acgcttcaag ccgcctggga tcgctcggac atcgcctccc tgcgtgccat gatgaccgac
      301 agcatgctgg gggagataca agcccagttg accgagcggg agaagctcac tggcagtacg
      361 cccaatttca ccgaggtggt gatgatcgaa gcgcgcttgc tggggatcga agaaatggcc
      421 aatgaataca tggccagcgt cgagttttcc ggaatgatcc gggaagagcc ctcagcgggt
      481 cccaacccct tccgcgaggt ctggaacatg accaaggcac gcaatggcgg cagcggctgg
      541 ctggtggcag gagtgcaggc gctgcaataa gcctgcaggc gcggaatagc gcaccatctg
      601 gggaataatg ggggactatg gcaacacagt cccctttttc cttgctggaa ggtcttttcc
      661 agagtttcaa tctccccatg actccgcctg cctgggccgt ggatgaggcg catcgcaaga
      721 cggtgttgct gctcaaccat gtattgcagc aggagcctca agccatggag cgcctggccc
      781 ggcagaaggg ccgcgtggta ctcagccagt ggcgggaatt cagtttcaaa gtactggtca
      841 ccccggcagg tttgctggac cttgcgtcgg cagacgcggc ctcggatctg acgctggtgg
      901 tgacggagca atcgccgttg gctattgccc aatcggtgat gcagggtgac aaacccgccg
      961 tgcgcattga gggtgatgta caactcgcgg ccgaaatcaa ttggctggcc gaccatgtgc
     1021 gctgggacct cgaagaagac ctggcccgcg tattgggcga tgcgccggcc cacatggtgg
     1081 tgcaggctgg ccgtaccctg gcacagggcc tgggtcaatt tgttggcaag cgcacccccg
     1141 catgacacgg atctaccgcg gcatctttat cgtctgggtg gccctgcgtt acgggctgga
     1201 tgagctggtt ctcaccagtt ttcagaagcc ctggctgcgg ctggtggcgc gcatcgtttc
     1261 cgtaggacgc aacctggagg cgccgcgcgg tcagcgcctg cgtgaggcac tggagcggct
     1321 ggggcccatt ttcgtcaagt tcggccaggt gctgtcgacc cgacgggatc tcttgcctct
     1381 ggacatagcc gacgaactgg cgaagctgca ggatcgcgta ccgccattca gctccgcagt
     1441 ggccgtggcc accatcgagc gcgaattgcg caagccactg aaccagatct tcgtttcttt
     1501 tgaacagatt ccagtggcca gcgcgtcgat tgcacaagtg cacttcgcgg tcctgcgtga
     1561 ccgcaagggc caggaacgtg aggtggcagt caaggtgctg cgccccggca tggccaaggt
     1621 catcgacaag gatttgagcc tgatgcgcat gatggccaaa tggctggagc ggctgtcagc
     1681 cgatggcaag cggctcaagc cacgtgaggt ggtcgcggag ttcgacaaat atctgcatga
     1741 cgagctggac ctggtgcgtg aagcgtccag cgccgcccag ttgcgccgca acatggatgg
     1801 cctgaatctc gtgctgattc ccgaaatgtt gtgggattac tgctctaccg aagtactggt
     1861 gatggagcgc atgcatggag tgcctatcag ccaggtagac cggttgcgtg cggccggcgt
     1921 ggatatcccc aagctggcac gcgatggcgt gaccatcttc ttcacccagg tttttcggga
     1981 cggtttcttc cacgcagaca tgcacccggg caacatccag gtcagtctgg ccccggaaac
     2041 ctttgggcgc tatatctcgc tggacttcgg catcgtcggc acgctgaccg aggtggacaa
     2101 agagtatttg gcgcagaatt ttgtggcttt tttccggcgc gactacaagc gcgtggcaga
     2161 actgcacatc gaatccggtt gggtcccttc cagcacacgg gtcgacgaac tggaggctgc
     2221 ggtgcgtgcc gtctgcgagc cctatttcga ccggccgctg aaggaaattt cgctgggcat
     2281 ggtgctgatg cgcctgttcc aggcgtcccg tcgcttccat gtcgagatcc agccgcaact
     2341 ggtgctgttg caaaaaacac tgctcaacat cgaaggcctg gggcgcgagc tggacccgga
     2401 actggacctc tgggcgactg ccaagccttt cctggagcaa tggatgctcc agcaggtcgg
     2461 gccccagagg ctcatcgagg agctcaagga ccatgcgccg cggtacgccc ggctgttgcc
     2521 cgagttaccg ggactcatcc aggcctacct gcgccgcaac ggttcagaca atgctgtgct
     2581 ggtgcaagag cttctgctgg agcagaggcg taccaatcgc ctgctgcaga cactggtgtc
     2641 ggccgtgatc ggcttcgccc tgggcatggt tgtcatgcag attgtgataa gggtaggggt
     2701 tttctagtac gcaccttata attgaaggct ttgcaacctg acgccagagc cctttcacca
     2761 tgcccatcta cgcctataaa tgcgagtcct gcgggtttgc caaggacgta ttgcagaaaa
     2821 tttctgacgc cccgctggag cagtgcccca gctgcagcca gcctaccttc aaaaagcagt
     2881 tgaccgctgc cggattccag ctcaagggtt ccggctggta cgccacggac ttcaagggtg
     2941 gggccacctc gacgtctgcc accgcgacgg caactgaagg cgccgctgcc tcgcccaagg
     3001 ccgacgcggc accttccact ccggcagcgc ctgctgcctc tacctgatcg ttgccgtgcc
     3061 catcaaaaaa tacctgctta ccgggctgat ggtctggctg ccgctggcca tcaccatctg
     3121 ggtgttgttg tggctggtcg gcatgctcga cgccgtattc ggcggttttc tgtccgttct
     3181 ggctgcggtc accccggccg cttttgcccc tgtgatcgaa cgcctgcacc acattccggg
     3241 tctgggcgtg ctgctggtgt tttcggtcat gctggttacc ggtgcgctgg ttgccaacgt
     3301 agcgggccgc tggtggatca agcaatggga ccggctgttt accaacatcc ccatcgtcaa
     3361 atccatctac aacagtgtca agaaggtttc tgacacgctg ttctccagca atggcaatgc
     3421 gtttcgtacc gccatgctga tccagtatcc gcgtgcgggc agctggacca ttgcgttcca
     3481 gacgggcgtg cccagcggag aggtttcgtc ccacctggga agcgacttcg tgagcgtcta
     3541 tgtgcccacc acacccaatc ccaccagcgg tttctttttg atggtgccca gagccgacgt
     3601 gatcgaactg gacatgagcg tggaccaggc gctcacttat gtcatctcca tgggctcggt
     3661 cgccccgtca cagcatctgc ccaaagcagt gcctcccaag taaccaatcc acaacccgct
     3721 gcctgaaggc ggcgtccaga aagcaagtta tggccatgcg ctcccactat tgcggtcttg
     3781 tgaccgaagc cctgatgggc gaaaccgttt ccctgtgcgg ctgggtcaac cgtcgccgtg
     3841 accatggtgg cgtgatcttt gtcgatctgc gtgaccgcga aggctatgtg caggtggtct
     3901 gcgaccccga ccgtgctgac atgtttgcag tggccgaaga cgttcgcaac gagttctgcg
     3961 tgcaggtcaa aggtgtggtg cgtgcacgac ccgaaggcac tgccaacgac aacctcaaga
     4021 gtggcaagat cgaggtgctg tgccacgaac tgatcgtgct caatgcctcc gtcacacctc
     4081 cgttccagct ggacgacgac aacctgtcgg aaaccacccg cctgacgcac cgcgtgctgg
     4141 acctgcgccg cccgtacatg cagaacaacc tgatgctgcg ttaccgcgtg tccatggaag
     4201 tgcgcaagtt cctcgatgcc aatggcttcg tggacatcga aacgcccatg ctgaccaagt
     4261 ccacgcccga aggcgcgcgc gactacctgg tacccagccg cgtgcacgat ggccacttct
     4321 tcgcgctgcc ccagtcgccc cagctgttca agcagctgct gatggtggcg ggctttgacc
     4381 gttactacca gatcaccaag tgcttccgcg acgaagacct gcgcgctgac cgccagcccg
     4441 aattcaccca gatcgatatt gaaacttctt tcatggaaga gcaggacatc cgcgacatgt
     4501 tccagggcat gatc
//
LOCUS       DAOSJM010000192         4506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17020_length_4506_cov_4.373175, whole genome shotgun sequence.
ACCESSION   DAOSJM010000192 DAOSJM010000000
VERSION     DAOSJM010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17020_length_4506_cov_4.373175"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="PKX82_07810"
     CDS             <1..461
                     /locus_tag="PKX82_07810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOF52198.1"
                     /translation="IHAQGEGFIRTLADRLERGAIFLLDYGFGESEYYHPQRHMGTVM
                     CHQAHKSDGNPLVDVGLKDITAHVNFTGIAVAAQEAGLEVLGYTNQAHFLINCGLAQA
                     MEQAPLQARVAAHKLITEHEMGEFFKVIGLCKGPHWDAVGFARGDRTHRL"
     gene            532..969
                     /gene="trxC"
                     /locus_tag="PKX82_07815"
     CDS             532..969
                     /gene="trxC"
                     /locus_tag="PKX82_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HOF52199.1"
                     /translation="MTESLHIVCPHCHTTNRVKTADMQNAPDCGGCHKPLFEAHSTAL
                     DEAAFDRHIGRSQIPVLVDFWAPWCGPCRQMAPAFEQAAAQLEPHVRVAKVNTEEAQN
                     LAARFNIRSIPTLALFVNGREVARQPGAMGAADIVRWTQSHLR"
     gene            1014..1382
                     /locus_tag="PKX82_07820"
     CDS             1014..1382
                     /locus_tag="PKX82_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HOF52200.1"
                     /translation="MQTTTRTETIERTQRPWGWYETVSEVPGNKIKRICVLPGQQLSL
                     QKHRQRSEHWVVVQGTARVTLDERVFDLHPGEHCDIAMGQVHRIANVTDLPVEVVEVQ
                     FGSYLGEDDIVRLQDDYGRR"
     gene            1425..2423
                     /locus_tag="PKX82_07825"
     CDS             1425..2423
                     /locus_tag="PKX82_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOF52201.1"
                     /translation="MTALSTRSALSPEPFTGLPKGAAPVACVDIGGTKVAVSIADEHG
                     VRGKVSEPTAKEGRNDALALQIIRLIGQSCAAAGVPVAAISAVGVASCGPFVIHQGMV
                     ELAAPNICGGMAGKARGLPNDWPTALLEAPLRAAFPNVRVENDGVGALEAERRWGALQ
                     GMDHCAYVTWSTGIGMGLCVDGHILRGKNGNAGHAGHTFVSDNDDALCGCGSIGDVEA
                     LVAGNAIPRRFGSLGYTDAAMLFVASGAGDARAAAIIDDLCRVMGRTLFNMIATLDLQ
                     RISIGGSVFWHHRDLLLPKVMGHVQGKLGALTHGVEIVPAGLGERVGDYAALALVA"
     gene            2580..3830
                     /locus_tag="PKX82_07830"
     CDS             2580..3830
                     /locus_tag="PKX82_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF52202.1"
                     /translation="MLKLSKLATAVALVVAGSAAMAGEVEVLHWWTSGGEAKSVAELK
                     TIMQGKGHTWKDFAVAGGGGDNAMTVLKSRVVSGNPPAAAQIKGPALQEWAAEGVLAN
                     LDATAQAEKWDSLLPKVVADGMKYKGNYIAVPVNVHRLNWTWANAEVLKKSGVKAMPT
                     TWDEFFAASEKIKKAGFIPVATGGNSWNDFTTFETVALGVGGVKFYKDALVNLDQKAL
                     TSDAMKKSLEAFRKLKSYTDAGAVGRDWNLATAMVIQGKAGFQFMGDWAKGEFVAAGK
                     VPGKDYICAAAPGTAKSYSYNIDSFAMFKLKDPAAQKAQADLAAAIMGTQFQEVFNLN
                     KGSIPVRLKMDMAKFDDCAKLSAKDFVETAKNGNLVPSVSQSMAVSPAAEGALKDAVS
                     KFWNDDKLSVADAIKNIAKAGKTK"
     gene            3976..>4506
                     /locus_tag="PKX82_07835"
     CDS             3976..>4506
                     /locus_tag="PKX82_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOF52203.1"
                     /translation="MKTFENLLPKLVIAPGFVLGFGFIYGFMIWNGVLSVTGSRMLPN
                     YDEFVGLEQYIRLWDMDRWYVALKNLGIFSTLYVGGSMALGMVLAIFLDQKVRAEGFL
                     RTVYLYPMALSFIVTGTAWKWILNPSLGLEKLMHDLGWSSFSFDWLVQSDTAIYCVVI
                     AGIWQSAGFAMALFLAG"
BASE COUNT          888 a         1291 c         1413 g          914 t
ORIGIN      
        1 aaatccacgc ccagggcgag ggtttcatcc gcaccctggc cgaccggctg gagcgcggcg
       61 ccatcttctt gctggactat ggttttggcg agagtgagta ctaccacccg cagcgccaca
      121 tgggcaccgt gatgtgccac caggcgcata agagcgatgg caatccgctg gtggatgtgg
      181 ggctgaaaga catcaccgca cacgtgaatt tcaccggcat tgcggtagcg gcgcaggaag
      241 ccgggctgga ggtgctgggc tataccaacc aggcgcactt tctgatcaat tgcggcctgg
      301 cccaggccat ggagcaggcg cccctgcagg cccgcgtggc cgcgcacaag ctaatcaccg
      361 agcatgagat gggcgagttc ttcaaggtca tcggcttgtg caagggcccg cactgggacg
      421 ccgtgggctt tgcccgaggc gaccgcaccc atcggctctg actccccttg cctttccggc
      481 ccttgaagtc gggcaatgga tacctatatg ggtaccaaac aaggctctac catgaccgaa
      541 tcactgcaca ttgtctgccc ccattgccac accaccaacc gcgtgaaaac tgcggatatg
      601 cagaacgccc ccgactgcgg tggttgccac aagccgctgt ttgaggcgca ctccactgcg
      661 ctggacgaag cggcgtttga ccgccacatt ggccgcagcc agattccggt gctggtggat
      721 ttttgggcac cgtggtgcgg cccgtgccgc cagatggcac cggcgttcga gcaggccgca
      781 gcccagttgg aaccccatgt gcgagtggcc aaggtcaata ccgaagaggc gcaaaacctg
      841 gccgcgcgct tcaacatccg cagcattccg acgctggcgc tgttcgtgaa tggccgcgaa
      901 gtggcgcgcc agcccggtgc catgggcgca gccgacatcg tgcgctggac gcagtcgcat
      961 ctgcggtaac cggcaggtaa cgcgaaaggc gaatggcggg tagcattccc gccatgcaaa
     1021 ccaccacccg caccgaaacc atcgaacgca cccagcgtcc ctggggctgg tacgagaccg
     1081 tgtccgaggt gccgggcaac aagatcaagc ggatctgtgt actccccggc cagcagctca
     1141 gcctgcagaa gcaccgccag cgctcggagc actgggtggt ggtgcagggc accgcgcggg
     1201 tcacgctgga cgagcgcgta ttcgatctgc acccaggtga acactgcgat atcgcgatgg
     1261 gacaggtgca ccgcattgcc aatgtgaccg acctccccgt cgaagtggtg gaggtgcagt
     1321 ttggcagtta cctcggcgaa gatgacatcg tacgtttgca ggatgactac ggccgccgtt
     1381 aagctcgggc cttgttctct ttccccattt gactgcgcac cgccatgacc gctttatcca
     1441 cccgttccgc cctttctcca gaacccttta ccggactccc caagggggct gcccccgttg
     1501 cctgcgtgga cattggcggg accaaggtgg cggtgagcat tgccgatgag cacggcgtac
     1561 gcggcaaggt ttccgagccc acggccaaag agggccgcaa tgacgccctg gccctgcaga
     1621 tcatccgctt gattggccag agctgtgcgg ccgcaggtgt gcccgtggcg gccatttctg
     1681 cggtgggcgt ggcctcgtgt gggccttttg tgatccatca gggcatggtc gaactggctg
     1741 cgcccaacat ctgcggcggc atggccggca aagcccgcgg cttacccaat gactggccca
     1801 ccgcgctgct ggaggcgccg cttcgcgcgg ccttccccaa cgtgcgggtg gaaaacgatg
     1861 gcgtcggcgc actggaagcc gagcgccgct ggggcgcgct gcagggcatg gaccactgtg
     1921 cctacgtgac ctggagcact ggcataggca tgggtctgtg tgtggacggt catatcctgc
     1981 gcggcaagaa cggcaatgct ggccatgcag gccacacctt tgtcagcgac aacgacgacg
     2041 cgctgtgcgg ctgcggcagc attggcgatg tggaggcgct ggtggctggc aatgccatcc
     2101 cccggcgttt cggttcgttg ggttacaccg atgctgctat gctttttgta gcttccggtg
     2161 caggtgatgc gcgggctgca gccattattg atgacctgtg ccgggtaatg gggcggaccc
     2221 tgttcaacat gatcgccacg ctggacctgc aacgcatcag catcggcggc agcgtcttct
     2281 ggcaccaccg cgacctgctg ctgcccaagg tgatgggcca tgtgcagggc aagctggggg
     2341 cattgaccca cggcgtggaa attgtccccg ctggcctggg tgagcgggtg ggggactacg
     2401 ccgcgctggc gctggtggcc tgacgtccga taccctgggt ccacctaggg tatgccccag
     2461 tgttcataaa gctttaatta atgaataatt aacgcatcct ggcctgcatg gtgctgactg
     2521 gggttcaacg ccgtccggag ttctgtttcg gggctgggac gcaatatttg ggagacatga
     2581 tgttgaagct ttctaaactc gccaccgctg tggcattggt agttgccggc tctgctgcaa
     2641 tggctggcga agtggaagtt ctgcactggt ggacttcggg cggtgaagcc aagtccgttg
     2701 cagaactcaa gaccatcatg cagggcaagg gccacacttg gaaagacttt gccgtggccg
     2761 gtggcggcgg cgataacgcc atgaccgtac tcaagagccg cgttgtgtcg ggcaacccac
     2821 ctgctgcagc ccagatcaaa ggacctgctc tgcaagagtg ggccgctgaa ggcgtattgg
     2881 ccaatctgga tgccacggcg caagctgaaa aatgggatag tttgcttccc aaggtggtag
     2941 cagatggcat gaaatacaag ggcaattaca ttgctgtccc agtcaatgtg caccgtctca
     3001 actggacgtg ggccaatgca gaggtgttga agaagtcggg tgtcaaggct atgccaacga
     3061 catgggatga gttctttgcc gcatctgaaa aaatcaagaa ggctggcttt attccagttg
     3121 ccactggtgg caattcctgg aatgatttca ctacttttga gacagttgca ttgggcgtgg
     3181 gcggagtcaa gttttacaag gatgcattgg taaatctgga ccaaaaggca ttgaccagtg
     3241 atgccatgaa aaagtcgttg gaggcattcc gaaagctcaa gagttacaca gatgcgggtg
     3301 cagtaggacg agactggaac ctggccactg cgatggtcat acaaggcaag gccggattcc
     3361 agttcatggg agactgggcc aagggtgagt tcgtagctgc cggcaaagtg cctggcaaag
     3421 attacatctg cgccgcggct cccggaactg cgaagtccta tagctacaac atcgactcat
     3481 ttgccatgtt caagctgaaa gaccccgctg cccagaaggc gcaggctgat ctcgctgcgg
     3541 ccatcatggg tacccagttc caggaagtgt tcaacctgaa caagggttcc atccccgtgc
     3601 gcctgaaaat ggacatggcc aagtttgacg actgtgccaa gctctccgcc aaagactttg
     3661 ttgaaaccgc caagaatgga aatctggtac cttccgtttc acaaagcatg gcggtaagcc
     3721 ctgcggcaga gggtgctctc aaagatgccg tcagcaagtt ctggaacgac gacaagcttt
     3781 ctgtggcaga tgcaattaag aatattgcaa aagctggcaa gaccaagtaa gcgtttcgtt
     3841 cgcccgcaga ggggagggga tgcgtaccgc cgttcgtgtc ccccgccctg cgggctcctc
     3901 ctttacctca ctctggcacg caccccaccc cttccgctgg agcggggtgc tttggggtaa
     3961 caccgcgtca cgcccatgaa aacatttgaa aacctgttac ccaagctggt gattgcgccg
     4021 ggctttgtgc tcgggtttgg cttcatctac ggcttcatga tctggaacgg cgtactgtcc
     4081 gtgaccgggt cgcgcatgct gcccaattac gacgagttcg tggggctgga gcagtacatc
     4141 cgcctgtggg acatggaccg ctggtatgtg gccctcaaga acctgggcat tttcagcacc
     4201 ttgtatgtcg gcggctcgat ggcgctgggt atggtgctgg cgatttttct ggaccagaaa
     4261 gtgcgtgccg aaggtttcct gcgcacggtg tacctgtacc ccatggcgct gtcgttcatc
     4321 gtgaccggca cggcctggaa atggattctc aatcccagcc tggggttgga aaagctgatg
     4381 catgacctgg gctggagcag cttcagcttt gactggctgg tgcagtcgga cacggccatt
     4441 tattgcgtgg tgattgccgg catctggcag tcggccggtt ttgccatggc gctgtttctg
     4501 gcgggc
//
LOCUS       DAOSJM010000193         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17227_length_4465_cov_4.577778, whole genome shotgun sequence.
ACCESSION   DAOSJM010000193 DAOSJM010000000
VERSION     DAOSJM010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17227_length_4465_cov_4.577778"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1395
                     /locus_tag="PKX82_07840"
     CDS             52..1395
                     /locus_tag="PKX82_07840"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOF52204.1"
                     /translation="MKRRTVLQHGSVVLALGLPHIAFGATILNVRVWPAPEYSRVTIE
                     SDTELHARQTFVPSPPRLAVDIDGITLNPALKELVAKVKADDPNIAGIRVGQFTPTTV
                     RLVIDLKLPIRPQVFALTPVAAYQHRLVFDLYPEKEVDPLEALIADRLKGTAPAPAAV
                     ASAPKTAADPLGDLIAQQTHKGSAAPPPGATSSVAPSAGSTGAGGTERLIIIALDPGH
                     GGEDPGAIGPAGTREKDVVLRLAHLLRDRINTTTVKGNPMRAYLTRDADFFVPLQVRV
                     QKARRVQADLFVSLHADAFFTPDPQGASVFALSQGGASSSAARWMAAKENKADLIGGL
                     NVRAKDATVQRALLDMSTTAQINDSLKLGKTLLGEISRVGKLHKPRVEQASFAVLKAP
                     DIPSVLVEAAFISNPTEEAKLNSDEYQNRLADALMRGIVGYFSRNPPLARNRTTS"
     gene            1411..2676
                     /locus_tag="PKX82_07845"
     CDS             1411..2676
                     /locus_tag="PKX82_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HOF52205.1"
                     /translation="MDFSSPVWQTRATLIGLCVAIGIGLLIGAERERRKTSTQDRSAA
                     GIRTFTVAALLGAVGALVGGVALVGVAVLLVGAGALIAYQRTRSTDPGMTTEFALVLT
                     CLLGALAIRDALLAAGIGAILALLLAARNRIHHFVRSVLSEQELHDIILFSATALIVL
                     PLAPDRYMGPFGAINPHALSRLVVVVMAISAAGHVAMRWLGPKYGLPIAGFASGFVSS
                     AATIHAMGQKASSNPAIMGAAVSGAVLSSVSTIVQMAVVIAMVQPGLLKPMATPLLLG
                     GAVAALYALVILARSTREQDPAQAMAPEHAFNLKTSVAFAALLSVVLVLSAGLNASLG
                     DAGMLLGAAASGFADAHATAASAASLLHANKIGLHQAMTAILVGLTTNTATKAFLAVH
                     SGGRTYARRILPGLAAMLLALWLGFWLAL"
     gene            complement(2683..3327)
                     /locus_tag="PKX82_07850"
     CDS             complement(2683..3327)
                     /locus_tag="PKX82_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOF52206.1"
                     /translation="MEILAALIDFILHVDKHLELFVQNYGLWVYALLFAIVFVETGLV
                     VMPFLPGDSLLFVVGALAGAGMLSYPVAVAVLLVAAVLGDQCNYSIGRYFGPKVFQWE
                     DSRFFNRQAFYQAHNFYEKYGGVTIILARFMPFIRTFAPFVAGVAEMSRVQFTLYNVV
                     GAVVWVLGICTAGYFLGSVPWVKLNLDKIIWAMILVPGLVILAGAWKARHKKPA"
     gene            3363..>4465
                     /gene="mutL"
                     /locus_tag="PKX82_07855"
     CDS             3363..>4465
                     /gene="mutL"
                     /locus_tag="PKX82_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOF52207.1"
                     /translation="MNAVFNPTPRRPIRELPDELISQIAAGEVVERPASVVRELVDNA
                     LDAGATQVTVRLLAGGVRLISVEDNGQGILRDELPIAFKRHATSKIGNLHDLESVSTM
                     GFRGEALAAINSIADCAILSRASGLNDAYLLDGRTGELKPVARSTGTTVEVKELFYST
                     PARRKFLKTDATELAHCIEAVRRHALARPDVGFAIWHEGKLVEQWRKSDSLDQRLADV
                     LGADFLEQSIPVEWHSSATGTPVRVWGRAGLPDAARSRADQQFCYVNGRFVRDKVLTH
                     AARSAYEDVLHGHKQPVYALYLAIDPTRVDVNVHPTKIEVRFRDSREVHQAVRHAVED
                     ALAAPRAGTAAQDAPHDASKPSPLGLYAPKVPLA"
BASE COUNT          824 a         1534 c         1377 g          730 t
ORIGIN      
        1 ggcccacacc gccaccggtg ccgccctgct ggctgcggcg ggaggcgcca catgaaacgc
       61 cgcaccgtac tccagcatgg cagcgtggtg ctggcgctgg gcctgcccca catcgccttt
      121 ggcgcgacca tcctcaatgt gagggtatgg cccgcgcccg agtattcgcg ggtcaccatc
      181 gaatccgaca ccgaactcca tgccaggcag acctttgttc ccagcccgcc gcgtctggcg
      241 gtggacattg atggcatcac gctgaaccct gcactcaagg aactggtggc caaggtcaag
      301 gccgacgacc ccaacatcgc cggcatccgc gtgggccagt tcacgcccac cacggtgcgt
      361 ctggtcatcg acctcaagct gccgatccga ccccaggtgt tcgccctgac accggtggca
      421 gcctaccagc accggctggt gtttgacctg tatcccgaga aagaagtcga cccgctcgaa
      481 gcactgattg ccgaccgcct caagggcacg gcgcccgccc ccgcagccgt cgcgtccgcc
      541 cccaaaaccg cggccgatcc gctgggcgac ctgattgcgc aacagaccca caaggggtct
      601 gccgctccac ccccaggcgc tacatcttct gtagcaccta gcgcaggttc cacgggggct
      661 ggaggcacag aacgcctgat catcatcgcc ctggacccag gccacggcgg cgaagacccg
      721 ggcgccatcg gaccggcggg cacccgcgag aaagatgtgg tgctgcgcct ggcccacctg
      781 ctgcgcgacc gcatcaacac caccaccgtc aagggcaacc cgatgcgtgc ctacctgacg
      841 cgcgacgccg acttctttgt gcccctgcag gtgcgggtgc agaaggcccg ccgggtgcag
      901 gccgacctgt ttgtgagcct gcatgccgat gccttcttca cgcccgaccc gcagggcgcc
      961 agcgtgtttg ccctgagcca gggcggcgcg tcgagcagtg ccgcgcgctg gatggcagcc
     1021 aaggaaaaca aggccgacct gattggcggc ctcaatgtgc gcgccaagga cgctacggtg
     1081 cagcgcgccc tgctcgacat gagcaccacc gcgcagatca acgacagcct caaactcggc
     1141 aaaaccctgc tgggtgaaat cagccgcgtg ggcaagctgc acaagccgcg ggtggagcag
     1201 gcctcttttg cggtgctcaa ggcaccggac attcccagcg tgctggtgga ggctgccttc
     1261 atcagcaacc cgaccgagga agccaagctc aacagcgatg aataccagaa ccgtctggcc
     1321 gatgcgctga tgcgtggcat cgtcgggtac ttctcccgca atcccccgct ggcgcgcaac
     1381 cgcacgacca gctagaccgg accgccacgc atggactttt cctccccggt gtggcagaca
     1441 cgtgcaaccc tgatcgggct gtgcgtggcc atcggcattg gcctgctcat tggcgccgaa
     1501 cgggaacgcc gcaagacctc cacccaggac cgcagtgccg caggcatccg caccttcacg
     1561 gtggccgcgc tgctgggggc ggtgggcgcc ctggtggggg gcgtggccct ggtgggcgtg
     1621 gccgtgctgc tggtgggtgc cggcgccctg attgcctacc agcgcacccg cagcactgat
     1681 ccgggcatga ccaccgagtt cgcgctggta ctgacatgcc tgctgggtgc cctggccata
     1741 cgcgacgccc tgctggccgc cggcatcggc gccatcctgg cactgctgct ggccgcacgc
     1801 aaccgcatcc accactttgt gcgcagcgtg ctgtccgagc aggagttgca cgacatcatc
     1861 ctgttttcag ccaccgcgct gatcgtgctg cccctggcac ccgaccgcta catgggcccc
     1921 tttggcgcca tcaaccccca cgccctgtcc aggctggtgg tggtggtcat ggccatcagc
     1981 gcggcgggcc atgtggcgat gcgctggctc ggtcccaaat acgggttgcc cattgcaggg
     2041 tttgcgtccg gttttgtgtc gagtgccgcc accatccacg ccatgggcca gaaagccagc
     2101 agcaacccgg cgatcatggg ggctgcggtg tcgggcgcgg tgctgtcctc ggtgtccacc
     2161 atcgtgcaga tggccgtggt aattgccatg gtgcaaccgg gattgctcaa acccatggcc
     2221 acaccgctgc tgctgggcgg cgccgtggcg gcgctgtacg ccctggtcat tctggcccgc
     2281 agcacacggg agcaggatcc ggcccaggcc atggcaccgg aacacgcctt caacctcaag
     2341 acgtcggtgg cttttgccgc actgcttagc gtggtgctgg tcctgtctgc cgggctcaat
     2401 gcctcgctgg gcgacgccgg catgctgctg ggtgcggcag cctctggttt tgccgatgcc
     2461 cacgccactg cagcctcggc ggcgtccctg ctgcatgcca acaagatcgg tctgcaccaa
     2521 gccatgaccg cgattctggt cggcctgacc accaacacgg ccaccaaggc cttcctggcg
     2581 gtgcactccg gtggccgcac ttacgcgcgc cgcatccttc cgggcctcgc ggccatgctg
     2641 ctggcactat ggctggggtt ctggctggcg ctgtagcggc gctcaggcgg gctttttgtg
     2701 gcgggctttc caggcgccag cgagaatcac taaaccgggg acaaggatca tggcccaaat
     2761 gattttgtcg aggttcagct tcacccaggg cacgctgccc agaaagtagc ccgcagtgca
     2821 aatgcccagt acccagacca cggcacccac aacgttgtag agcgtgaact gcacgcgact
     2881 catttcggca acaccagcca cgaacggagc gaaggtgcgg atgaacggca taaagcgagc
     2941 cagaatgatg gtgacgccgc cgtacttttc atagaaattg tgggcttggt agaacgcctg
     3001 ccggttgaaa aagcgcgaat cctcccactg gaacaccttg gggccgaaat accgcccgat
     3061 ggagtaattg cactggtcgc ccagcacagc cgccaccaga agcacagcca cagctaccgg
     3121 atagctcaac atcccggcac ccgccagcgc gcccactacg aatagcagcg aatcgcctgg
     3181 cagaaagggc atgacgacca gaccggtctc cacaaaaacg atggcaaaca gcagcgcata
     3241 gacccacaga ccgtagttct gcacgaacag ctccaggtgt ttgtcgacgt gcaggatgaa
     3301 gtcaatgagc gcagcaagga tttccatgga ggcagattat ccctgcaccc taaaatggtc
     3361 tcgtgaacgc agtcttcaac cccactcccc ggcggcccat ccgcgaactt cccgacgaac
     3421 tcatcagcca gattgccgct ggcgaggtgg tcgaacgccc ggcctcggtg gtgcgcgaac
     3481 tggtggacaa cgcgctggac gcgggtgcca cgcaggtgac ggtacggctg ctggccggtg
     3541 gcgtgcgcct gatcagcgtg gaagacaacg gccagggcat cctgcgtgac gagttgccca
     3601 tcgccttcaa gcgccatgcc accagcaaga ttggcaacct gcacgacctg gaatcggtgt
     3661 ccaccatggg tttccgcggt gaggccctgg ccgctatcaa ttcaatagct gactgcgcaa
     3721 tactgtcgag ggctagcggc ctgaatgatg cctatctgct ggacggacgc acgggtgaac
     3781 tcaagccggt ggcgcgcagc accggcacca cggtcgaagt gaaggagctg ttttacagca
     3841 cccccgcacg ccgcaaattc ctcaagaccg acgccaccga gctggcccac tgcattgaag
     3901 ccgtgcgccg ccatgccttg gcccgacccg atgtgggctt tgcgatctgg cacgaaggca
     3961 agctggtcga acagtggcga aagagtgaca gcctggacca gcgcctggcc gatgtgctgg
     4021 gcgccgattt tctggaacag agcattccgg tggaatggca ttcgtctgcc accggcacac
     4081 cagtgcgcgt ctggggccgc gccggcctgc cggacgccgc ccgctcccgc gccgaccagc
     4141 agttctgcta cgtcaacggc cgcttcgtgc gcgacaaggt gctcacccac gccgcccgca
     4201 gcgcctacga agacgtgttg cacggccaca aacaaccggt gtatgcgctg tacctggcta
     4261 tcgacccgac ccgggtggac gtgaacgtgc accccaccaa gatcgaggtg cgctttcgcg
     4321 acagccgcga agtgcaccag gcggtgcgcc acgccgtgga agacgcgctg gcggcaccgc
     4381 gtgcaggcac cgcggcgcag gatgcgccgc acgatgcctc caaaccttct ccactcgggc
     4441 tttatgcacc caaagtgcct ctagc
//
LOCUS       DAOSJM010000194         4409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17499_length_4409_cov_4.019982, whole genome shotgun sequence.
ACCESSION   DAOSJM010000194 DAOSJM010000000
VERSION     DAOSJM010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17499_length_4409_cov_4.019982"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1140
                     /gene="dusB"
                     /locus_tag="PKX82_07860"
     CDS             91..1140
                     /gene="dusB"
                     /locus_tag="PKX82_07860"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOF52208.1"
                     /translation="MHIGHFAMANPLFVAPMAGVTDRPFRQLCKRLGAGYAVSEMVTS
                     RRDLWNTLKTARRANHDGEPGPIAVQIAGTEAQMMADAAVYNIERGAQIIDINMGCPA
                     KKVCNKWAGSALMQDEALALSIVEAVVAACAPRNVPVTLKMRTGWCQAHRNAVTLARA
                     FEAAGVQMLAVHGRTREQGYKGEAEYDTIAAVKAAVQIPVVANGDITSPEKARDVLQA
                     TGADAIMIGRAAQGRPWIFREIAHFLATGAHLAPPLVNEVKRLLVEHLHDHYSLYDEF
                     IGVRSARKHIGWYVRELPGGEEFRSAMNALHDSVAQVAAVEQYFDGLNEKMDRIPVIG
                     DLANTRGRDMTECVV"
     gene            1137..1373
                     /locus_tag="PKX82_07865"
     CDS             1137..1373
                     /locus_tag="PKX82_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF52209.1"
                     /translation="MSKKLLEESVRTSLEGYFRDLRGTEPGGMHDMLVRAVEKPLLEV
                     VMAQADHNQSKAAQWLGLNRNTLRKKLLEHRLID"
     gene            1401..2999
                     /gene="purH"
                     /locus_tag="PKX82_07870"
     CDS             1401..2999
                     /gene="purH"
                     /locus_tag="PKX82_07870"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOF52210.1"
                     /translation="MNALLSVSDKTGIVELAQALHAQGIKLLSTGGTAKLLAEKGLPV
                     TEVADMTGFPEMLDGRVKTLHPRVHGGLLARRDVPAHMAALKEHAIGTIDFLVVNLYP
                     FEATVAKAGCTLEDAIENIDIGGPAMVRSAAKNWKDVAVLTDASQYATVIAELKASGQ
                     VSRKTKFALGVAAFNRISQYDGAISDYLSAVQVGEDAPADAEAPKLERFPGQSNGRFI
                     KVQDLRYGENSHQSAALYRDLYPAPGSLVTAKQLQGKELSYNNIADADAAWECVKSFK
                     TPACVIVKHANPCGVAVGANALESYSKAFQTDPTSAFGGIIALNTELDEAAAQQISKQ
                     FVEVLMAPSFTAGALDVFKSKVNVRILQIALPKGGSSDWDNGRNAMDMKRIGSGILLQ
                     TADNHELTLADLKVVTTKQPTQDELNDLLFAWNVAKFVKSNAIVFCKGGMTMGVGAGQ
                     MSRLDSARIASIKAGHANLSLAGTVVASDAFFPFRDGLDVVVDAGATCIIQPGGSMRD
                     DEVIAAANERGVAMVFSGVRHFRH"
     gene            3109..3516
                     /locus_tag="PKX82_07875"
     CDS             3109..3516
                     /locus_tag="PKX82_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52211.1"
                     /translation="MTTPTKRAAPARAKPAAKSTVPIKASSEPFLRFYHSKELRARTL
                     KVLSTLEACDDPTEHCGVLTDLILELVDSGMDYYFLRSLKATGAGFVAQQSASLGLVG
                     VKKVMGTVIRNVLGRMSGPQLLSVCATLRELME"
     gene            complement(3595..>4409)
                     /locus_tag="PKX82_07880"
     CDS             complement(3595..>4409)
                     /locus_tag="PKX82_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF52212.1"
                     /translation="GHATSAGVPAEVVQHTLVLEYNNVAQLEEAFALHGKELACLIIE
                     PIAGNMNFVRASVPFMKRCRELCTEYGAMFVFDEVMTGFRVALGSAQSVYAKALPGFE
                     PDVTVMGKVIGGGMPLAAFGGKRAVMEQLAPLGPVYQAGTLSGNPVATACGLATLREI
                     RKPGFYEELSRKTQSFISGLQGAADKAGVDFCGDSQGGMFGFFLFKDLPQNYATVMTT
                     DNQRFNTLFHGLLDRGVYIAPALYEAGFMSAAHTDADIAEAVAAAADVFKLL"
BASE COUNT          886 a         1347 c         1366 g          810 t
ORIGIN      
        1 atttcaattg ctgaaatttt aggcagctag aatggtgccc gacccgcgtc accgctatcc
       61 aaccccaccc caattttctc aaacctccgc atgcacatcg gccacttcgc tatggcgaat
      121 cccctgtttg ttgcacccat ggcgggcgtg acggaccggc cttttcgcca gctctgcaaa
      181 cgcctgggcg caggctatgc ggtaagtgaa atggtgactt cgcggcgtga cctgtggaac
      241 accctcaaaa cggcacgccg tgccaaccat gacggtgagc ccggaccgat tgcggttcag
      301 attgcgggga ccgaagcgca gatgatggcc gatgccgctg tctacaacat cgagcgcggc
      361 gcgcaaatca tcgacatcaa catgggctgc ccggccaaga aggtctgcaa caagtgggcc
      421 ggctcggccc tgatgcagga cgaggcactg gccctgtcca tcgttgaagc ggtggtggca
      481 gcttgcgctc cgcgcaacgt gcccgtcacg ctgaagatgc gcaccggctg gtgccaggcc
      541 cacaggaatg cggtcacctt ggcgcgtgcg tttgaggcgg ctggcgtgca gatgctggct
      601 gtgcatggcc ggacccgcga gcaggggtac aaaggcgaag ccgagtacga caccattgcg
      661 gcggtcaagg ctgcggtgca gattccggtg gtggccaatg gtgatatcac atctcccgaa
      721 aaggcacgcg acgtattgca ggccaccggg gcggacgcca tcatgattgg ccgcgccgcg
      781 caaggaaggc cgtggatttt tcgcgagatc gcgcactttc tggcgaccgg cgcacacctg
      841 gcgccaccgc tggtgaacga ggtcaagcgc ctgctggtgg aacacctgca cgaccactac
      901 agcctgtacg acgaattcat tggtgtgcgc tcggcacgca aacacattgg ctggtacgtg
      961 cgggagttgc cgggaggcga ggaatttcgc agcgcgatga atgccttgca tgacagcgtg
     1021 gctcaggtgg cggcggtgga acagtatttt gatggtttga acgagaagat ggaccgcatc
     1081 ccggtaatcg gtgatttggc caacacaaga ggacgagaca tgacggaatg tgttgtatga
     1141 gcaagaagct cctggaagaa agcgtacgta ccagcctgga aggctacttt cgcgatctgc
     1201 gcggcaccga gcccggcggc atgcatgaca tgctggtgcg cgcggtggaa aagccgctgt
     1261 tggaagtggt gatggcgcag gccgaccaca accagtccaa ggccgcgcag tggctgggtt
     1321 tgaaccgcaa cacgctgcgc aaaaagctgc tggaacatcg cctgattgat tgattgattt
     1381 tttaacttag ctactccacc atgaacgcac tactttctgt ctctgacaaa accggcatcg
     1441 tcgaacttgc gcaggcgctg catgcacaag gcatcaagct gctctccacc ggaggcactg
     1501 ccaaactgct ggccgagaaa ggtttgccgg tgaccgaggt ggccgacatg accggcttcc
     1561 ccgagatgct cgacggccgt gtcaagaccc tgcacccgcg cgtgcacggt ggcttgctgg
     1621 cccgccgcga tgtaccagcc cacatggctg cgctcaagga gcacgccatc ggcaccatcg
     1681 acttcctggt ggttaacctg tatccgttcg aggccaccgt agccaaggct ggctgcacgc
     1741 tggaagacgc catcgaaaac atcgacatcg gtggcccggc catggtgcgc agtgctgcca
     1801 agaattggaa ggatgtggcg gtgctgaccg acgcgtcgca atacgccacc gtgattgccg
     1861 aactcaaggc cagcggccag gtgtcgcgca agaccaagtt cgccttgggt gtggccgcgt
     1921 tcaaccgcat cagccagtac gacggcgcca tcagcgatta cctttccgcc gtgcaggtgg
     1981 gtgaggatgc tccggccgac gccgaagccc ccaagctgga acggttcccc ggccagagca
     2041 atggccgctt catcaaggtg caggacctgc gctatggcga gaacagccac cagagcgccg
     2101 ccctgtaccg cgacctgtac cccgcccctg gctcgctggt caccgccaag cagctgcagg
     2161 gcaaggaact ctcctacaac aacattgccg acgccgatgc agcgtgggag tgtgtgaaga
     2221 gcttcaagac gccggcctgc gtcatcgtca agcatgccaa cccctgtggc gtggccgtgg
     2281 gcgccaatgc actggagtcc tacagcaagg ccttccagac cgaccccacc agtgcgtttg
     2341 gcggcatcat cgcgctgaac accgagctgg acgaagctgc agcccagcag atctccaagc
     2401 agtttgtcga agtgctgatg gcaccgagct tcacagctgg cgcgctggac gtgttcaaga
     2461 gcaaggtcaa tgtgcgcatt ctccagatcg cgttgcccaa gggcggctcc agcgattggg
     2521 acaatggccg caacgccatg gacatgaagc gcatcggctc cggcatcctg ctgcagaccg
     2581 ctgacaacca cgagctgaca ctggccgacc tcaaggtggt caccaccaag cagcccacgc
     2641 aagacgagct gaacgacctg ctgttcgcct ggaatgtggc caagttcgtc aagtccaacg
     2701 ccatcgtctt ctgcaagggc ggcatgacca tgggcgtggg cgccggccag atgagccgcc
     2761 tggactccgc gcgcattgca tccatcaagg ccggccacgc caatctgagc ctggccggta
     2821 ccgtggtagc cagcgatgcc ttcttcccgt tccgcgacgg tctggatgtc gtcgtcgatg
     2881 ccggtgccac ctgcatcatc cagcccggcg gctccatgcg cgacgacgag gtgatcgccg
     2941 ccgccaacga gcgtggcgtg gccatggtgt tcagcggcgt gcgccacttc aggcattgag
     3001 tttttgagcc tttttggcct ctagcccctg cggagcctgc gcatgcagct actgaattgg
     3061 tagcggtttg tgtgggtttt gtttttgtgg cggcgtatca tggctcacat gaccactccc
     3121 accaaacgcg cggcgccggc tcgcgccaag ccggctgcca aatccacggt gccgatcaaa
     3181 gcatcttcgg agccttttct tcgcttctac cactcgaaag aactgcgcgc caggacgctg
     3241 aaagtactgt cgaccctgga ggcatgtgac gaccctacgg agcactgcgg tgtgctgacc
     3301 gacctgatcc tggaactggt ggatagcggg atggactact acttcctgcg ctcgctcaag
     3361 gccaccgggg cgggattcgt ggcccagcaa tctgcgtccc tggggttggt cggcgtgaag
     3421 aaggtcatgg gcacggtgat tcgcaatgtg ctgggacgca tgagcgggcc gcaattgctc
     3481 tcggtctgcg ccaccttgcg cgagctcatg gagtgaacgg gcgcccaaag caaaagggct
     3541 gaatgtcaaa ttggcatcca gcccccgcac aatatgcgtg agtagctatt atatttatag
     3601 caacttgaaa acgtctgccg ccgccgctac ggcttcagca atgtcggcat cggtgtgcgc
     3661 agcactcatg aagcccgctt cgtaaagagc cggggcaatg tagacgccgc ggtccagcag
     3721 accgtggaac agcgtgttga aacgctggtt gtcggtggtc atgacggtgg cgtagttctg
     3781 cggcaggtcc ttgaacagga agaagccgaa catgccgccc tgcgagtcgc cacagaagtc
     3841 cacaccggct ttgtccgccg cgccttgcag gccgctgatg aacgattggg tcttgcggga
     3901 cagttcctcg taaaacccgg gtttgcggat ctcgcgcagg gtggccagac cacaggcggt
     3961 agccaccgga ttgcccgaca aggtgccggc ctggtacacc gggcccagcg gcgcgagttg
     4021 ctccatcacc gcgcgcttgc caccaaaggc cgccagcggc atgccgccgc caatcacttt
     4081 gcccatgacg gtgacatcgg gctcaaagcc ggggagggcc ttggcgtaca cgctttgtgc
     4141 gctacccagc gcgacgcgaa agccagtcat gacttcgtca aacacaaaca tggccccgta
     4201 ttcggtacac aactcgcggc agcgcttcat gaagggaacc gaggcacgca caaagttcat
     4261 gttgccggcg atgggctcga tgatcaggca ggccagttcc ttgccgtgca aggcaaaggc
     4321 ttcttccagt tgcgcgacgt tgttgtattc cagcaccagg gtgtgctgca ccacctcggc
     4381 cggtacgcct gcgctggtgg cgtggccaa
//
LOCUS       DAOSJM010000195         4407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17507_length_4407_cov_5.167969, whole genome shotgun sequence.
ACCESSION   DAOSJM010000195 DAOSJM010000000
VERSION     DAOSJM010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17507_length_4407_cov_5.167969"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="PKX82_07885"
     CDS             <1..1921
                     /locus_tag="PKX82_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF52213.1"
                     /translation="VSTMALSELFFTLYSDVADIFNLLGHVYKVIAYAFVYKSIFVDN
                     IQAPYQRLNAANRMLEQQIEERTRTQSQLELAASVFSHAREGILITDNQGNIVEVNAM
                     FTQITGYSREEAIGRNPRILLSSGRHEKAFFAARAQALQKHGHWVGEVWNRRKNGEDF
                     AEMLTVSVVRDPAGAVLNYVALFTDITPLKKHQDELEHIAHYDALTGMPNRVLLADRL
                     QQAMLQSQRRNSALAVAYLDLDGFKPVNDQHGHEVGDQLLVTLSQRMKASLRESDTLA
                     RIGGDEFVALLVDIGERRDCELLLSRLLQAAATPVRVDGALLQLSASIGVTLYPQDNA
                     DADQLVRHADQAMYLAKQAGKNRFHLFDVAQDAAIQSQRESLEHIRVALDRQEFVLYY
                     QPKVNMRTGAIIGAEALIRWQHPERGLLAPAAFLPVIEGQPISIEIGEWVIQTALAQM
                     ALWRGAGLDIGVSVNIGALQLQQPGFVARLGTLLAAHPTVPAQSLELEILETSALEDM
                     AQISEVMHACQAIGVRFALDDFGTGYSSLTYLKRLPAELLKIDQSFVRDMLTDVDDLA
                     IVKGVIGLASAFRRSVIAEGVETVAHGQTLLALGCDLAQGYGIARPMPAADLPGWARQ
                     WRPDVGWLDAADRGV"
     gene            complement(1926..3890)
                     /locus_tag="PKX82_07890"
     CDS             complement(1926..3890)
                     /locus_tag="PKX82_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52214.1"
                     /translation="MALITLLDAQLAFGHVPLLDHADFSLETQERVGLIGRNGAGKSS
                     MLKILGGMEKPDDGTLQVQSGTRISYVAQEPQLDADATIFIAVRAGLERVIGLIEQYC
                     AGEGDLDTMQSEIETLDGWNWEQRVNETLQRLHLNPEAIVSTLSGGTKKRVALAQALV
                     AQPDVLLLDEPTNHLDLDSIEWLEGLLKEFKGSIITITHDRSFLDNVATRIVELDRGK
                     LMSYPGNFAAYLTQKEEQLAQEAVINAKADKLLAQEEVWIRKGVEARRTRATARIVRL
                     DELRSQHAARRNAVGSVNMDVSSGDKSGKLVSEMVHVTKTFGDRVIVRDFSTTVLRGD
                     KIGLLGPNGAGKTTLLKLILGEIKPDPAPANAPPPEPGHSPWGTVRLGSNVTVAYFDQ
                     MRNALDLDATLEDFISPGSEWIEIGNQRKHVKSYLGDFLFSPARANSPVRSLSGGERN
                     RLLLARLFARPANVLVLDEPTNDLDIDTLELLEDLLQNYDGTVFLVSHDRTFLDNVVT
                     STIAFEGDAGWREYEGGVSDWLIQTKRANEINAALGKTTAKPFNYEREQLQKQEQAVA
                     QVSAATPAAPPPAKAKKLSFKEQRELDGLPDRIAALEAEQKDIASALADGSLFSSDNT
                     RALALTARNAAIDDELMAALERWEVLGRPA"
     gene            3940..>4407
                     /locus_tag="PKX82_07895"
     CDS             3940..>4407
                     /locus_tag="PKX82_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOF52215.1"
                     /translation="MPTLPVSLFDGLPGLSASDWGVVAVFAVVYLGMFLGGLPRLKLD
                     RSGVALLGAIAVIAITGESVEEAARTVDLPTIVLLFAFMVVSAQMRLGGFYGAVTQKV
                     ADMPLSRNGLLAALIVVAGALSAVFSNDVICLAMTPVVARLCLQRRLPPVPFLV"
BASE COUNT          723 a         1347 c         1448 g          889 t
ORIGIN      
        1 ggtgtccacc atggcattga gcgagctgtt ttttacgctg tattccgacg tcgcagacat
       61 cttcaacctg ctgggccatg tgtacaaggt gatcgcttac gcgtttgtct acaagagtat
      121 ttttgtggac aacatccagg cgccttacca gcggctcaat gccgccaacc ggatgctgga
      181 gcagcagatc gaagaacgca cccggacgca atcgcagctg gaactggccg ccagcgtgtt
      241 cagccacgca cgcgagggca tcctcatcac ggacaaccag ggcaatattg ttgaggtcaa
      301 cgcgatgttc acccagatca cggggtatag ccgggaagag gctattgggc gcaacccccg
      361 catcctgctg agttcgggtc ggcatgaaaa ggcttttttt gcagcccgtg cccaggcact
      421 gcaaaagcat ggccactggg tgggcgaagt ctggaaccgc cgcaagaacg gtgaggactt
      481 tgcggagatg ctcaccgtca gcgtggtgcg cgacccggca ggcgccgttc tgaattacgt
      541 ggcgctgttc accgacatca cgcccctcaa gaagcaccag gacgagctgg agcatattgc
      601 ccattacgac gccttgaccg gcatgcccaa ccgcgtgctg ctggccgacc gcctgcagca
      661 ggccatgctg cagagccagc gcaggaacag tgcattggcg gtggcctacc tggaccttga
      721 cgggttcaag cccgtcaatg accagcacgg acatgaagtg ggggaccagt tgctggtgac
      781 actgtcccag cgcatgaagg ccagtctgcg cgagagcgac acgctggcac gcatcggtgg
      841 cgatgaattt gtggccttgc tggtagatat cggggagcgc agggactgcg agctgctgct
      901 gtcgcggctg ctgcaggccg ctgccacgcc cgtgcgggtg gatggcgcac tgctgcagct
      961 gtcggccagc attggtgtca cgctgtaccc gcaggacaac gcggatgccg accagctggt
     1021 gcgccatgcc gaccaggcaa tgtatctggc caagcaggcg ggcaagaacc ggttccacct
     1081 gttcgatgtg gcgcaggatg ctgccatcca gagccagcgc gagagcctgg aacacatccg
     1141 tgtggcgctg gaccggcagg agttcgtcct ctattaccag cccaaggtca atatgcgcac
     1201 cggggccatc atcggcgccg aggccctgat ccgctggcag caccccgagc gcggtttgct
     1261 ggcgccggcg gcatttctgc cggtcataga ggggcagccg atcagcatcg aaatcggcga
     1321 atgggtgatt cagacggcac tggcccagat ggccctgtgg cgtggtgcgg gtttggacat
     1381 cggcgtcagc gtcaacatcg gtgcgctgca gttgcagcaa cccggatttg tggcgcgtct
     1441 gggcacgctg ctggcggccc atcccacggt gccagcgcag agcctggagc tggaaattct
     1501 ggaaaccagc gccctggagg acatggccca gatctcggaa gtgatgcatg cctgccaggc
     1561 catcggtgtg cgctttgcgc tcgacgattt tggcaccggc tattcctcgt tgacctacct
     1621 gaagcggctg cccgccgaat tgctcaagat cgaccagagc tttgtgcgcg acatgctgac
     1681 cgacgtggac gatctggcca tcgtcaaagg ggtgatcggg ctggcttctg ccttccggcg
     1741 cagcgttatt gcggagggcg tggaaaccgt ggcccatggc cagaccctgc tggctctggg
     1801 ttgcgatctg gcacaggggt acggcattgc caggcccatg cccgccgccg acctgccggg
     1861 ctgggcacgc cagtggcgac cggatgtagg ctggctggac gccgctgacc gcggcgtcta
     1921 ggcactcagg ccgggcggcc cagcacttcc cagcgctcca gcgctgccat cagttcgtcg
     1981 tcaatggcag cattgcgcgc tgtcagcgcc agcgcacggg tgttgtcgct ggagaacagg
     2041 ctgccatcgg ccagcgcgct ggcaatgtct ttctgctcgg cttccagtgc ggcaatgcgg
     2101 tcgggcaggc catcgagttc gcgctgttct ttgaagctca gtttcttggc cttggccggt
     2161 ggcggtgctg ccggagtggc ggccgacacc tgggctacgg cctgctcctg tttttgtagc
     2221 tgctcacgct catagttaaa gggcttggcc gtggttttgc ccaatgccgc attgatttcg
     2281 ttggcccgct tggtctggat caaccagtcg ctcacgccgc cttcgtattc gcgccaacca
     2341 gcatcgcctt caaaggcaat cgtgctggtg accacgttgt ccaggaaggt ccggtcgtgg
     2401 ctgaccagga acaccgtgcc gtcgtagttt tgcagcagat cttccagcag ttccagcgtg
     2461 tcgatgtcca ggtcgttggt cggctcgtcc agcaccagca cgttggcggg gcgcgcgaac
     2521 aggcgcgcca gcagcaggcg gttgcgctcg ccaccggaga gcgaacgcac cggcgagttg
     2581 gcgcgcgccg gcgagaacag gaagtcgccc aggtagctct tgacgtgctt cctctggttg
     2641 ccaatctcga tccactccga gccggggctg atgaagtctt ccagcgtggc gtccaggtcc
     2701 agcgcgttgc gcatctggtc gaagtaggcc actgtcacat tgctgcccaa gcgcactgtg
     2761 ccccaggggc tgtggcccgg ttcgggtggc ggtgcgttgg cgggtgccgg gtcgggcttg
     2821 atctcgccca gaatcagttt gagcagcgtg gtcttgcctg cgccgttggg gcccagcagg
     2881 ccaatcttgt cgccacgcag tacggtggtg gaaaagtcgc gcacgatcac gcggtcgccg
     2941 aaggtcttgg tcacatgcac catctcggag accagcttgc cgctcttgtc gccgctggac
     3001 acgtccatgt tcacgctgcc cacggcgttg cgccgcgcgg catgctggga gcgcagttcg
     3061 tccaggcgca cgatgcgggc ggtggcgcgg gtgcggcggg cctccacgcc cttgcgaatc
     3121 cacacctctt cctgtgccag cagcttgtcg gccttggcgt tgatcacggc ttcctgcgcc
     3181 agctgctctt ccttctgggt caggtaggcg gcaaagttgc cggggtagct catgagcttg
     3241 ccgcggtcca gttcgacgat gcgcgtggcc acgttgtcca ggaaggaccg gtcgtgcgtg
     3301 atggtgatga tggagccctt gaattccttg agcaggcctt ccagccactc gatcgagtcc
     3361 aggtccaggt ggttggtcgg ctcgtcgagc agcagcacat cgggctgggc caccagcgcc
     3421 tgcgccagcg ccacgcgctt cttggtaccg ccggacaggg tgctgacaat cgcctcgggg
     3481 ttcaggtgca ggcgctgcag ggtctcattg acgcgttgct cccagttcca gccgtccagg
     3541 gtttcaatct cgctctgcat ggtgtcgagg tcaccttccc ctgcgcaata ttgctcaatc
     3601 aggccgataa ccctctccaa gcctgcgcgg acagctatga aaatagtagc gtctgcatcg
     3661 agttgcggtt cctgtgccac gtaggaaatg cgggtgccgc tttgcacttg cagggtgccg
     3721 tcgtcgggct tttccatgcc gcccagaatt ttgagcatgg aggacttgcc cgcgccattg
     3781 cggccaatca gacccacgcg ctcctgcgtt tccagagaaa agtcagcgtg gtcgagcagg
     3841 gggacgtgcc caaatgccag ctgggcgtcg agtagggtga taagtgccat agggttccgg
     3901 tgaagccccc gattatccct tgcaactatg atttgccgca tgcccaccct gcctgtatcc
     3961 ctctttgacg gcctgccggg cctgagcgcg tccgactggg gtgtggtggc cgtgttcgcc
     4021 gtggtgtatt tgggcatgtt cctcggcggg ctgccgcgcc tcaaactgga ccgctcgggc
     4081 gtggcgctgc tgggcgccat tgcggtgatc gccatcaccg gagagtcagt cgaggaagcg
     4141 gcccgcaccg tagacctgcc caccatcgtg ttgctgtttg ccttcatggt ggtgtccgcg
     4201 cagatgcgtc tcggtggctt ctacggcgca gtgacgcaga aagtggccga catgccgctg
     4261 tcacgcaacg gcctgttggc cgcgctgatc gtggtggcag gcgcgttgtc agccgtgttt
     4321 tccaacgatg tgatctgcct ggccatgaca ccggtggtgg cgcgcctgtg cctgcagcgc
     4381 aggctgccgc cggtgccgtt tctggtg
//
LOCUS       DAOSJM010000196         4391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17590_length_4391_cov_4.011993, whole genome shotgun sequence.
ACCESSION   DAOSJM010000196 DAOSJM010000000
VERSION     DAOSJM010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17590_length_4391_cov_4.011993"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKX82_07900"
     CDS             <1..314
                     /locus_tag="PKX82_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOF52216.1"
                     /translation="TGDASLKNLSGPITIADYAGKSASMGVSQFAIFLALISVSLGVL
                     NLLPLPVLDGGHLMYYLWESVTGRPPSAVWEERLQKFGLAVLMMMMSVALFNDISRLL
                     G"
     gene            361..2658
                     /gene="bamA"
                     /locus_tag="PKX82_07905"
     CDS             361..2658
                     /gene="bamA"
                     /locus_tag="PKX82_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOF52217.1"
                     /translation="MLLNREFLRSALCTVAFGVCAQVAWALEPFQVRDIRVEGLQRVE
                     AGTIFASLPVKVGDTYSDDKAAASIRSLFALGLFTDVRIEAVGGVLVVIVEERPSIAE
                     VDFSGTKEFDKDVLKKALRDIGLTDGRPFDKALVDRAEQELKRQYINRSMYATEVITT
                     VTPIERNRVNLSFAVIEGDTAKISDIRIVGNKAFADSTLLGMFDQDTGGWLSWYTKSN
                     RYSRTKLNADIEILRSHYLTRGYLEFKVDSTQVAISPDKQSIAITINITEGERFIVSR
                     VKLAGNYLGKDEEFQSLISIKAGEPYNVDQVAQTTKAFTDYFGNFGFAFARAEARTSI
                     DRATNRVEITLQADPSRRAYVRRINVAGNDRTRDEVIRREFRQLEAAWYDGDKIRQSR
                     NRVDRLGFFREVTLDTQEVPGSPDQVDLTVNVVEKPTGSISLGAGLSSADGFGLTFGF
                     KQENAFGSGSSLGVELNTSKYNRTAVFNTTNPYFTEDGVSRTIDLYQRTSKPYTDIDS
                     YSIETSGASVRFGVPFTDVDTVYLGVGVERTTIVTGTYLPTVYQDFAKEFGSTANAFP
                     LTVGWSRDTRDSALVPSTGKVMRTTGEWSVAGDLRYARGTVQYQQFFPVSRKVTAAIN
                     GELSLGAGTDGLSYPVFKNYYSGGLGSIRGFEQGSLTTGTQRAQGLIATGGTKKITLN
                     TEVLSPLPGGGNDRTLRMFGFFDAGGVFGADESIAVADFRTSVGVGISWISPVGPLRL
                     AFAKPISHFDGDKMQTLQFQIGTTF"
     gene            2658..3167
                     /locus_tag="PKX82_07910"
     CDS             2658..3167
                     /locus_tag="PKX82_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOF52218.1"
                     /translation="MKTLKTSLCAGLLMAACALSAVAQESKIGYVNTQRITSESGPAK
                     TATAKLEQEFSKRQKELSDAGASLKSFSDKFERDAPTMTESQRVSKQKEFAEMNRDFQ
                     RKQREFQEDLNGRRNEELQQVLDKANKAVKVVAEAEKYDLIIQEVVYNNGKHDITDKV
                     LKILNAGAK"
     gene            3192..4166
                     /gene="lpxD"
                     /locus_tag="PKX82_07915"
     CDS             3192..4166
                     /gene="lpxD"
                     /locus_tag="PKX82_07915"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOF52219.1"
                     /translation="MELSLSEVVGLLGGDVYGQTDMVLTGIAPLETAEQGDVSFLSNP
                     KYQQQLQSSRAGCVIVGPAMREAAMARGTCIVTDQPYLYFARLTQLWKRKRNAAILPS
                     IHPSAVVDPSATVHSSASVGPLCVVERGARIGAGTVLKSRVTVGEDCSIGERCILHSG
                     VVVGADGFGFAPNGETWVKIEQLGAVQIGNDVEIGANTCIDRGALQDTVIEDGVKLDN
                     LIQIGHNVRVGRNTAMAGCAGVAGSATIGPNCTVGGGAIVLGHLTLGANVHISAATVV
                     SRSILKPGNYTGMFPIDDNASWERNAATLKQLHSLRDRIKTLEKETKK"
     gene            4163..>4391
                     /gene="fabZ"
                     /locus_tag="PKX82_07920"
     CDS             4163..>4391
                     /gene="fabZ"
                     /locus_tag="PKX82_07920"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOF52220.1"
                     /translation="MMDIHKILKQLPHRYPFLLVDRVLELDKGKTIKALKNVTINEPF
                     FEGHFPHRPVMPGVLMLEALAQAAALLAFDAL"
BASE COUNT          937 a         1196 c         1283 g          975 t
ORIGIN      
        1 tgaccggaga cgcctcactg aaaaatctga gtggccccat caccatcgca gactacgccg
       61 gtaaatctgc gtccatggga gttagccaat ttgccatctt tttggcgctg atcagtgtca
      121 gtctgggcgt tctcaacctt ctgccgctac ccgttctgga tggaggacac ctgatgtatt
      181 atctttggga gtcagtgacc gggcgacctc cgtctgcagt ctgggaagag cggctgcaga
      241 agtttggtct cgctgtgttg atgatgatga tgtcggtcgc gctttttaac gacatcagtc
      301 gcctgctggg atgaccgttg gtgcatccct acgtgatttg gaccatttca ttggacaacc
      361 atgctattga atcgtgaatt cttgcgcagt gccttgtgca ctgtggcttt tggtgtttgt
      421 gcgcaggttg cctgggctct tgaacccttt caagtgcgcg atattcgcgt cgaagggctg
      481 cagcgcgtcg aggcgggaac catctttgcc tccttgccag tcaaggttgg ggacacctat
      541 agcgacgaca aagccgcagc atccatccgc tccctgtttg ccctgggtct tttcacagac
      601 gtccgcattg aagcagtcgg cggagtgctg gtggtgattg tggaagagcg tcccagcatt
      661 gcggaagtgg acttttctgg caccaaggaa ttcgacaagg atgtcctcaa gaaggcgctg
      721 cgcgacattg gcctgaccga tggacggcct tttgacaagg cattggtgga tcgcgccgag
      781 caggagctca agcgccagta catcaaccgc agcatgtatg caaccgaggt gatcactacg
      841 gtgacgccga ttgagcgcaa ccgcgtcaac ctcagttttg cagtcatcga gggcgatact
      901 gccaagatca gtgacatacg cattgtcggc aacaaggcat ttgccgactc cacattgctg
      961 ggtatgtttg accaggatac cggtggctgg ctgagctggt acaccaagtc caaccggtat
     1021 tcccgcacca agctcaatgc cgacatcgag atattgcggt cccattatct gacacggggc
     1081 tacctggagt tcaaggtgga ttccacacag gtggccattt cgccagacaa acaaagtatc
     1141 gccatcacca tcaacatcac ggaaggggag cgctttatcg tttcccgcgt caaactggcg
     1201 ggcaactacc ttggcaagga cgaggaattc cagtcactga tatccatcaa ggccggtgag
     1261 ccctacaacg tggatcaggt cgcgcaaacg accaaggcct ttaccgacta tttcggcaac
     1321 ttcggttttg cttttgccag ggcggaagcg cgtacctcca ttgaccgtgc aaccaaccgg
     1381 gtggaaatta cactgcaggc cgatccttcc cgtcgcgcct atgtacgccg tatcaacgtg
     1441 gcaggcaatg accgcacgcg cgatgaagtg atccgccgtg agttccgaca actcgaagct
     1501 gcctggtacg acggtgacaa gattcgccag tcgcgcaatc gcgtcgaccg gctgggattc
     1561 ttccgggaag tgaccttgga tacccaagag gtgccgggtt ctcccgacca ggtggacctg
     1621 acggtcaacg tggtggaaaa acccactggc agtatcagtc tgggtgcggg tctttccagt
     1681 gccgatggct tcggtctgac attcggcttc aagcaggaaa acgcgttcgg ttctggcagt
     1741 tcgctgggtg tggagttgaa taccagcaag tacaaccgta cggccgtgtt caatacgacc
     1801 aacccctatt tcaccgaaga tggtgtctcg cgcaccattg atctgtacca acgtacgtcc
     1861 aagccctaca cggatattga cagctattcc attgagacat ctggtgccag cgtccgcttt
     1921 ggtgttccgt ttacggacgt ggatactgtg tacttggggg ttggggtaga gcgaacaacg
     1981 attgtcactg gcacctattt acctacggtt taccaagatt ttgcgaagga gttcggctcc
     2041 actgccaatg cctttccgtt gactgtgggt tggtcgagag acacccgcga cagtgctctg
     2101 gtgcccagta ccggcaaggt gatgcgcact acgggtgagt ggagtgtggc cggtgatttg
     2161 cggtatgcgc gcggcacagt ccagtaccaa cagttttttc cggtctccag aaaggtgacg
     2221 gccgctatca atggtgagct gtctctgggg gcgggtactg atggcctgtc gtatccggtg
     2281 ttcaagaatt actattcagg tggtttgggc tccatccgtg gattcgagca aggcagtctg
     2341 acgacaggta cacagcgggc acagggcctc attgctaccg gtggcaccaa gaagatcaca
     2401 ctcaatactg aggtgctatc gcctttgcca ggtggcggca atgaccgtac gctgcgcatg
     2461 ttcgggttct tcgatgcggg tggcgtgttc ggtgcggatg aatctatcgc ggtggcggac
     2521 ttcaggacct cggtgggcgt tggcatcagc tggatttcgc ccgttggtcc cctgcgcctg
     2581 gcttttgcca aacctatcag ccatttcgat ggcgataaaa tgcagacatt gcaatttcag
     2641 attggaacga ctttctaatg aagactctca agacatccct gtgtgcgggt ctgttaatgg
     2701 cagcctgtgc actgagcgca gtggcgcagg aatccaagat tggctacgtc aacacccagc
     2761 gtattacgtc ggagtctggc ccggccaaga ctgccacggc caagctggaa caggagttct
     2821 ccaagcgcca gaaagagttg tctgacgcag gtgcttccct gaagtccttt agcgacaagt
     2881 tcgaacggga tgcgcctacg atgaccgaaa gtcagcgcgt ttccaagcaa aaagaatttg
     2941 ccgaaatgaa ccgcgacttt cagcgcaagc agcgtgagtt tcaggaagac ttgaacggcc
     3001 gccgcaatga agaattgcag caggttctgg acaaggccaa caaggccgtc aaggtggtag
     3061 ccgaagccga aaagtatgac ctgatcatcc aggaagttgt gtacaacaac ggcaagcacg
     3121 acatcactga caaggttctc aagatcctca acgcgggtgc caagtaagtc ttggttctcc
     3181 aggtgagtgg catggaactc tccctgtccg aagtggtcgg gctgttgggg ggagatgtct
     3241 atgggcagac tgacatggtg ctcaccggca tcgctccgct ggagacagcg gaacaaggtg
     3301 acgtcagttt cctgagcaac cccaaatacc agcagcagtt gcagtcctcc cgtgccggat
     3361 gcgtgattgt ggggcccgcc atgcgggagg ccgccatggc gcgcggcacc tgcatcgtca
     3421 ccgaccagcc ttatctctat ttcgcgcgcc tgacacagct gtggaagcgc aagcgcaatg
     3481 cggccatcct gccgtccatt catccttctg ccgtggttga ccccagtgcc acggtgcact
     3541 ccagtgcctc cgtcgggccg ctctgcgtgg tcgaacgcgg cgcacgcatc ggtgccggca
     3601 cagtattgaa atcgcgtgtc accgtgggcg aagactgctc cattggtgag cgttgcatcc
     3661 tccattcggg cgtcgtcgtc ggtgcggatg ggtttggctt tgctccgaac ggtgagacgt
     3721 gggtaaaaat tgaacaactg ggggcggtcc agatcggcaa cgatgtggaa atcggcgcca
     3781 acacctgtat tgaccggggt gcgctgcagg acaccgtcat tgaggatggt gtcaagctcg
     3841 acaacctgat ccagatcggc cacaacgtac gcgtcgggcg caatactgcc atggcgggat
     3901 gtgcgggcgt ggctggcagt gccaccattg gacccaattg caccgtgggt ggtggcgcca
     3961 tcgtcctggg acatctgacc ctgggggcca atgtgcatat ttcggcggct accgtggtca
     4021 gtcgctctat cctgaagcct ggcaactaca ccggcatgtt cccgattgat gacaacgcgt
     4081 cctgggagcg caacgccgcc acactcaagc aattgcacag tctgcgcgac cgaatcaaaa
     4141 ccttggaaaa agagacaaag aaatgatgga catccacaaa attctcaaac agcttcccca
     4201 ccgctatccc ttcttgctgg tggaccgtgt cctagagctg gacaaaggca agaccatcaa
     4261 agcgctcaag aatgtcacca tcaacgagcc gttctttgaa ggccattttc cgcaccgtcc
     4321 ggtcatgccg ggcgtgttga tgctggaagc gctggcgcag gccgctgcct tgctggcgtt
     4381 tgatgcgctg a
//
LOCUS       DAOSJM010000197         4378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17671_length_4378_cov_3.338654, whole genome shotgun sequence.
ACCESSION   DAOSJM010000197 DAOSJM010000000
VERSION     DAOSJM010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17671_length_4378_cov_3.338654"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKX82_07925"
     CDS             <1..463
                     /locus_tag="PKX82_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOF52221.1"
                     /translation="FKNLTNTFITHIPYRGAGPALNDTVAGQVPMTFDNMPSAMPFIT
                     SNRLIPVVVAAPQRLAQLPNVPTFKEVGLEPVNRMAFYGIYGPKGMPKEIVDKINAAV
                     RKVLEDPAVKKRIEDTGSLIIGNTPEQFGAQIKAEYEVYKKVVDSAKLRLE"
     gene            460..1374
                     /gene="xerD"
                     /locus_tag="PKX82_07930"
     CDS             460..1374
                     /gene="xerD"
                     /locus_tag="PKX82_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOF52222.1"
                     /translation="MTSAALASQQAVDTFIDALWLEEGLSRNTLAAYRRDLTLYANWL
                     AGQSRTLDTTSEHDLQSYFAARHAATKATTANRRLTVFKRYFRWALREGAIQVDPTLK
                     LQSAKQALRVPKTLTESQVEALLHAPDVSGALGVRDRTMLELMYASGLRVSELVTLKT
                     FHVSLNDHVLKILGKGNKERLVPFGEVAGQWLRQYLGSARPELLAGKQTEDMFVTARG
                     SKAGSAMSRVMFWMVVKKYAQAAGIHAPMSPHTLRHAFATHLLNHGADLRSVQMLLGH
                     ADISTTTIYTHVARERLAALHAQHHPRG"
     gene            complement(1392..2654)
                     /locus_tag="PKX82_07935"
     CDS             complement(1392..2654)
                     /locus_tag="PKX82_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOF52223.1"
                     /translation="MSTPNPLTTLPTGQALAPIAIARQAILDEGRNVFGYELFDRSVV
                     SNEHTASSDAQLLFNVLSLSDSEALPGKKTIFINCTHDSLAGGHLDLVAPDRVVLEIP
                     PLPISKADQIPDYLPSLQEIQRRGFRMAFNYSILTRSYESWLPLASFIKFDLSILKPD
                     AVGSFIKLAQAKSQARLIAEKVETLKQYELVAGLGIKLFQGYWFAQPVMVEGQTIRPA
                     QATILQLINLVRKQASTNEIEEVLKRDPTLSFNLLRFINSAGFGMRTEVTSFKHAVML
                     LGLNRLFKWAALLMTTSRSGDTPPAVGTTAVIRGRLMELLATEILSVDDCDNAFVVGI
                     FSLLDTMLGIPMEQALATVMLPASVTDALLHKTGPLAPFLELTIACETGDDVAFANAA
                     NTLGLNSNQINWAHLQALAWAETLTQVD"
     gene            complement(2684..3475)
                     /locus_tag="PKX82_07940"
     CDS             complement(2684..3475)
                     /locus_tag="PKX82_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOF52224.1"
                     /translation="MELRAGAFAAFCLNDPADKVRATLALWTDKTDIPIDVQARFAPP
                     PEPGRPARPALVHPQEVARRSPFTPDGHAALIHSITHIEFNAINLALDAMWRFEGMPV
                     AYYLDWLQVAQEEAKHFTLLRNHLLEAGYDYGDLAAHDGLWTMCENTRHDVIARMALV
                     PRTLEARGLDATPIIQAKLRKVATPAALAAITILDLILEEEVGHVAIGNRWYHWLCAR
                     DSVDPEAFYTRAALEHDAPRLKPPFNILARRRAGFSESDIAALPV"
     gene            3514..4038
                     /locus_tag="PKX82_07945"
     CDS             3514..4038
                     /locus_tag="PKX82_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOF52225.1"
                     /translation="MAIYELDTIAPRLADSAWVADSAQVMGNVELGEDASVWFGAVIR
                     GDTEVIRIGQRSNIQDGSVLHADIGKPLTIGDDVTVGHKVMLHGCTIGDGSLVGIGAV
                     VLNGARIGKGCIVGAGSLVTEGKEFPDGSMIMGSPAKVVRTLTPEQQQGLVMSAVHYV
                     DNARRFKVGLHKLA"
     gene            4051..>4378
                     /locus_tag="PKX82_07950"
     CDS             4051..>4378
                     /locus_tag="PKX82_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOF52226.1"
                     /translation="MSELHKFLFEGLPVRGMVVRLTDAWTEILRRRASNTSSGPYAAP
                     VQEMLGEMTAAAALMQSNIKFDGSLVLQIFGDGPVKVAVVEVQSDLSLRATATVTEAV
                     SGDSRLS"
BASE COUNT          864 a         1216 c         1427 g          871 t
ORIGIN      
        1 gttcaagaac ctgaccaaca cctttatcac ccacattccc taccgcggtg caggccctgc
       61 gctgaacgac accgtggcgg ggcaggtgcc catgacgttt gacaacatgc catcggccat
      121 gcccttcatt acatcgaacc ggttgattcc ggtggtggtg gcagcacccc agcgcctggc
      181 gcagttgccc aatgtgccga ccttcaagga agtggggctg gagccggtga accgcatggc
      241 cttctatggc atttacgggc ccaagggcat gcccaaggag attgttgaca agatcaatgc
      301 cgctgtgcgc aaggtgctgg aagaccccgc cgtgaagaag cgcattgaag acaccgggtc
      361 actgatcatt ggcaatacac ccgagcagtt tggggcgcag atcaaggcgg agtacgaggt
      421 gtacaagaag gtggtcgata gcgccaagct gcgtctggaa tgacctcggc tgccctggcc
      481 agccagcagg ccgtcgatac ttttattgac gccctctggc tggaagaggg cttgtcccgt
      541 aacacactgg cggcttatcg gcgtgatttg accctgtatg ccaactggct ggcagggcag
      601 tcacggactc tcgataccac cagcgagcat gacctgcaat cctattttgc cgcgcgccat
      661 gcggcgacca aggccaccac cgccaatcgg cggctgaccg tgttcaagcg ctactttcgg
      721 tgggccctgc gcgaaggcgc aattcaggtg gaccccacgc tcaagctgca gtccgccaag
      781 caggccttgc gcgtacccaa gacgttgaca gaatcgcagg tggaagccct gctgcatgcg
      841 ccggatgtgt ccggggcgct gggggtgcgc gaccgcacca tgctggaact catgtacgcc
      901 agtggtctgc gcgtcagcga attggtcacg ctcaagacgt tccatgtcag cctgaacgat
      961 cacgtgctca agattctggg caaaggcaac aaggagcgcc tggtgccctt tggagaagtc
     1021 gccggacagt ggctgcgcca atacctgggc agtgccagac ccgagctgct ggcgggcaag
     1081 cagaccgaag acatgttcgt gacagcccgt ggcagcaagg caggcagtgc catgtcccgg
     1141 gtgatgttct ggatggtggt aaaaaagtat gcgcaggcag cggggatcca tgcgccgatg
     1201 tcaccacata ccttgcgcca tgcgtttgcc acccatctgc tcaaccacgg cgccgatttg
     1261 cgctcggttc agatgctgct gggccatgcc gacatttcga cgaccaccat ttacacccat
     1321 gtggcgcgtg agcgcctggc agcgctgcat gcgcagcacc atccgcgggg ctgaacccgc
     1381 ggacgaatgg gtcaatcgac ctgggtgagt gtttcggccc aggccagagc ctgcaggtgc
     1441 gcccagttga tctggttgct gttgagcccc agggtgttgg cggcattggc aaacgcgacg
     1501 tcatcgccgg tttcgcaggc gatggtcagt tccaggaagg gtgccagtgg cccggtcttg
     1561 tgcagcaggg catcggtcac ggaggcaggc agcatcacgg tggccagtgc ctgctccatc
     1621 ggtattccga gcatggtgtc cagcagcgaa aagatgccga cgacgaaagc gttgtcgcag
     1681 tcatcgaccg acaggatttc ggtggcgagc aattccatca ggcggccacg gatgaccgca
     1741 gtggtgccga ccgcgggggg ggtgtcgccc gagcgcgagg tggtcatcag cagggccgcc
     1801 cacttgaaca ggcgattcag gcccagcaac atcaccgcgt gcttgaacga ggtgacttcg
     1861 gtacgcatgc caaagcccgc cgagttgatg aagcgcaaca ggttgaacga gagcgtgggg
     1921 tcgcgcttga ggacttcttc aatctcgttg gtgctggcct gtttgcgtac caggttgatc
     1981 agttgcagga tggtggcctg cgccgggcgg atggtctggc cttcgaccat gacaggctgg
     2041 gcaaaccagt agccttggaa gagcttgatg cccagcccgg ccaccagttc gtactgcttg
     2101 agcgtctcca ccttttctgc aatcagccgg gcttgcgatt tggcctgtgc cagtttgatg
     2161 aaagatccca cggcatccgg tttgaggatg gagagatcaa acttgatgaa agaggccagt
     2221 ggcaaccagg attcgtacga ccgggtcagt atcgaatagt tgaacgccat ccggaagcca
     2281 cggcgctgga tttcctgcag gctgggcagg tagtccggaa tctggtctgc cttgctgatg
     2341 ggcagtggcg gaatttccag taccacccga tctggtgcca ccagatccag atgaccgcca
     2401 gccaggctgt catgggtgca gttgatgaag atggtttttt tgccgggcaa cgcttcgctg
     2461 tcgctgagcg agagcacatt gaacagcagt tgtgcgtcgc ttgaagcggt gtgctcattg
     2521 gacaccaccg agcggtcgaa cagctcgtac ccgaatacat tgcgtccctc gtccaggatg
     2581 gcctgacggg cgatggcaat gggtgccaag gcctggcccg tgggcagtgt ggtcaaagga
     2641 ttgggggtag acatggtgct ggcagggtgt atgtgggggg attctagacc ggcagggcag
     2701 cgatgtccga ttcgctgaac ccggcccggc gacgcgcaag aatattgaaa ggcggcttga
     2761 gccggggggc gtcgtgctcc agtgccgcac gggtgtagaa ggcttctggg tccacgctgt
     2821 cccgggcaca aagccagtgg taccagcggt taccaatcgc cacatgaccg acttcctcct
     2881 ccagaatgag atccagtatg gttatggcag ccagggctgc tggtgtcgcc acttttctga
     2941 gcttggcctg gatgataggt gtagcgtcca ggccgcgcgc ttccagggtg cgcggaacaa
     3001 gggccatgcg ggcgatgacg tcgtggcggg tgttttcaca catggtccac agtccatcgt
     3061 gggccgccaa gtcgccgtag tcgtaacccg cctccagaag gtggttgcgc agcagcgtga
     3121 agtgcttggc ctcctcctgg gccacctgca gccagtcgag gtaatacgct actggcatgc
     3181 cttcaaaccg ccacatggcg tcgagtgcga ggttgatggc gttgaactca atatgggtga
     3241 tggagtggat cagtgcggca tgaccatccg gagtgaatgg tgaacgccgt gccacctctt
     3301 gcgggtggac cagcgcgggc cgggctggac gcccgggttc tggaggcggg gcgaaccggg
     3361 cttggacgtc gatgggtata tcggttttgt ccgtccacaa ggccagcgtg gcacgcacct
     3421 tgtccgcagg atcattcagg caaaaagcag caaacgcacc ggctcgtagt tccatcccta
     3481 caattctaga cattgaaata gcaagggcag aacatggcaa tctacgaact cgacaccatt
     3541 gcaccccgct tggcggattc ggcctgggtg gctgacagcg cacaggtcat gggcaatgtg
     3601 gaactggggg aggacgcaag cgtctggttc ggcgcggtta ttcgcgggga taccgaggtc
     3661 attcgcatcg gccagcgatc gaacatccag gatggcagcg tgcttcatgc ggacattggc
     3721 aagcccctga ccattgggga cgatgtcacc gtgggtcaca aggtcatgtt gcatggatgc
     3781 actatcggcg acggttccct ggtgggtatt ggcgctgtgg ttctcaatgg tgcgcgcata
     3841 ggcaagggat gcatcgtcgg tgctggttcg ctggtgaccg agggcaagga gtttcctgac
     3901 ggctccatga tcatgggcag ccctgccaag gtggtcagga cactgacgcc agagcagcaa
     3961 cagggtctcg taatgagtgc ggtgcactac gtcgacaacg cacgccggtt caaagtcggc
     4021 ctccataaac tggcctgaag ggactgtagc gtgtctgaat tacacaaatt cctgttcgag
     4081 ggtttgcccg tgcgtggcat ggtggtgcgc ctgacggacg cctggacgga gatcctgcga
     4141 cgccgtgcat cgaataccag ttcgggtcct tatgcagcac ccgtgcagga gatgctgggt
     4201 gagatgactg ctgctgccgc cctgatgcaa tcgaatatca agttcgacgg ttcgctggta
     4261 ttgcagattt ttggtgacgg tccagtcaag gtcgcagtgg tggaggtgca atccgatctg
     4321 tccttgcgtg cgaccgctac cgttaccgag gccgtttccg gagacagccg gttgagcc
//
LOCUS       DAOSJM010000198         4344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17852_length_4344_cov_4.734204, whole genome shotgun sequence.
ACCESSION   DAOSJM010000198 DAOSJM010000000
VERSION     DAOSJM010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17852_length_4344_cov_4.734204"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PKX82_07955"
     CDS             complement(<1..1221)
                     /locus_tag="PKX82_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF52227.1"
                     /translation="MRHPPFTPQIRLYQNWLREQRGLTFDSYDALWRWSTTELDAFWQ
                     SIWDYFELRSPTPHRAALTVNRMPGAQWFPGAQVNYARQVLRHVDAAHAAGFPALISH
                     NEQSLAEGAQAQEISWPELRRRVASLALHLKAQGLQPGDRVAAYLPNIPEAMVAFLAV
                     VSLGGVWSICAPDMGTNAVLDRFTQIKPTVLIACDGVRFGGKDFDRRAVVSELCDALP
                     SVRHVLLHDNLGLPGIAIKSGAVCAAFSGACAQNDAEVTAFEPMWLPFDHPLWIVYSS
                     GTTGLPKPIVHGHGGTVVVALANKVLHNDVGCSYQPNSWGERFHWYSSTGWVMWNAQM
                     SGLLNGSTCCLFDGSPGGSTVDAAGNKVAPDWTTLWRFGANVGATFFGAGAAFFANCM
                     KAGIDLSQCGELRQV"
     gene            1319..2050
                     /locus_tag="PKX82_07960"
     CDS             1319..2050
                     /locus_tag="PKX82_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOF52228.1"
                     /translation="MASRPLRKTQRKTSSAAPAVEVRLDRKAAILLAAEKLFAQSGYH
                     AVTIRQIAEEADVPLALVSYYYGQKHELFEAIFAHWNATIEERMQELRDAETGPANAA
                     RLERIIRAFVGPVLRMRASAEGEYYALLVARELFYATEVTDRVLRVYFDPLANAFIDA
                     LHRIFAQATRGQMAWCYQFALGALMHHLSDTRVQRLSNHQSHPGDPTAQTMLVHFIAG
                     GMRAAIAQCDPRPKVTPLKQRRSLP"
     gene            2047..2985
                     /locus_tag="PKX82_07965"
     CDS             2047..2985
                     /locus_tag="PKX82_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF52229.1"
                     /translation="MKRRTALSSLAAAGLATTLPAWAQSTKIVFGYTAVTDFTSVFVA
                     AEEGYFKKRNLDVELKFIPLNSTIPAAIQSDSLQMGGPTPSVFLQSVDGGLDHVVIAG
                     GGVTSKTITGFGLVARAGSGIKTPQDCIGKKIGVPGLGAFLHVTFRAWLKDNKVDHKK
                     VNFVEASFPQHGDLLRGGSVDAVVTADPFMGRITDSGVGYVVSYYSTFLPEGNPTIIH
                     TAKREWAEKNAVAARAFRDAVVEGAAFMAKPANNAKVRAAIGKYIKLPPEVLAKAQIS
                     PPGPVVTEKQLTYWVGLMKDQEMLRTEPAVSRLIFK"
     gene            2996..3769
                     /locus_tag="PKX82_07970"
     CDS             2996..3769
                     /locus_tag="PKX82_07970"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF52230.1"
                     /translation="MSESPLLRFDQVGIHLGGREILSPTSFEVARGEFVCIIGPSGCG
                     KTTLLRAAAGFLQPSGGAALRHGKPITGPSREVAFVFQDYGRALLPWRTVAGNISLAL
                     EAAGVPAQQRPDRIAKVLDTVGLGAHATKFPAQLSGGMQQRVQIARCLAQEPELMMMD
                     EPFGALDAMTRESLQDEVSRLVRSTGLTVLFVTHDLEEAIYLSDRVIALRANPTADKP
                     SLAAMVEVKIARPRDQLLTKEHPEFLRLRRELYGFLGHH"
     gene            3769..>4344
                     /locus_tag="PKX82_07975"
     CDS             3769..>4344
                     /locus_tag="PKX82_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF52231.1"
                     /translation="MRPWVFPLLLLALLEAAARWLVPGSDALAPPSQALLAFGKALSA
                     SGDDSVLQATAFTLSSAALGLLLGAALGVTLGLVLGLSRRAAAMGFLSIEVLRPVPSV
                     ALIPLAMLVFGFGYRMEISVVAFATFWPLLILTQAAARQVEPQLLEVAHALGLSAWAR
                     TTQIVLPAMVPRLFIALRLGVSFALVVAVTVE"
BASE COUNT          780 a         1448 c         1334 g          782 t
ORIGIN      
        1 cacctgccgc aactccccac actgggacag gtcgatgccg gctttcatgc agttggcaaa
       61 gaacgccgca ccggcaccaa aaaacgtggc gcccacattc gctccaaagc gccacagcgt
      121 ggtccagtcc ggtgcaacct tgttgccggc agcatccacc gtgctgccgc ccgggctgcc
      181 gtcaaacagg caacaggtac tgccgttgag caagccgctc atctgtgcat tccacatcac
      241 ccagccggtg gagctgtacc agtggaagcg ttcaccccag gaattcggct ggtagctgca
      301 ccccacatcg ttgtgcagca ccttgttggc cagcgccacc accaccgtac cgccatggcc
      361 atggacaatc ggcttgggca aaccggtagt gccactggaa tagacaatcc acagggggtg
      421 gtcaaacggc agccacatcg gttcaaatgc ggtgacttca gcatcatttt gggcgcaagc
      481 ccccgagaat gctgcacaaa cagctcctga tttgatagca attccaggca accccaggtt
      541 gtcatgcagc agcacatggc gcacgctggg cagcgcgtcg cacaattcgg acaccacggc
      601 gcggcggtca aagtccttgc ctccaaagcg cacgccgtcg caggcgatca gcaccgtggg
      661 cttgatctgg gtgaaccggt ccagcaccgc gttggtcccc atgtccggcg cacaaatgct
      721 ccacacgccg cccaggctga ccaccgccag aaacgccacc atggcctcgg gaatattggg
      781 caggtaggca gctacccggt caccgggttg cagcccctgg gccttgaggt gcaacgccag
      841 cgatgccacc cggcgacgca gttcaggcca gctgatttcc tgcgcctgtg ctccctcagc
      901 caggctctgt tcgttgtggc tgatcagtgc cggaaatccg gccgcatggg ccgcgtcgac
      961 atggcgcagc acctggcgcg catagttcac ctgcgctccg ggaaaccact gcgcccccgg
     1021 catgcggttg accgtaagcg ccgcacggtg cggcgtgggt gagcgcagtt cgaagtaatc
     1081 ccagatgctt tgccagaagg catcgagctc ggtggtggac cagcgccaca acgcgtcgta
     1141 ggaatcaaac gtcaggccac gctgctcgcg cagccagttc tggtagagac ggatctgggg
     1201 agtaaaggga ggatggcgca tcctgcaagc gtagcaagac ttgccttgtg ccggtgggct
     1261 ttgcgctcag ggtttgtacg aatgttcaat tattttggac aagcgttcaa tgcattgcat
     1321 ggcatccaga cccctgcgca agacgcaacg caaaacctcc agcgccgcac cggcggtaga
     1381 ggtgcggctc gaccgcaaag ccgctatttt gctggccgcc gaaaagctgt ttgcacagtc
     1441 gggttaccac gccgtgacca tccgccagat tgccgaggaa gccgatgtac ctctggccct
     1501 ggtgagctac tactatggcc agaagcatga gctgttcgag gctatcttcg cccactggaa
     1561 tgccaccatc gaagagcgga tgcaggagct gcgcgatgcg gaaaccggcc cggccaacgc
     1621 cgcgcggctg gagcgcatca tccgggcatt cgtagggccg gttctgcgca tgcgggccag
     1681 tgccgaaggc gaatactacg cactgctcgt ggcacgcgaa ctgttctacg ccaccgaagt
     1741 caccgaccgg gtgctgcggg tctatttcga cccgctggcc aatgccttca tcgacgcact
     1801 gcaccgcatc tttgcgcagg cgacgcgcgg gcagatggcc tggtgttacc agtttgcact
     1861 cggcgccctg atgcaccacc tcagcgacac ccgggtgcag cgcctgtcca accaccagag
     1921 ccacccgggc gaccccacgg cgcaaaccat gctggtccat ttcattgctg gcggcatgcg
     1981 tgcggccatt gcgcagtgcg acccccgccc caaagtcacc ccactcaaac aacgaaggag
     2041 cctcccatga aacgccgtac cgccctctcc agcctggccg ctgctggctt ggccaccacg
     2101 ctgcccgcgt gggcccagtc caccaaaatc gtctttggct acaccgcggt gaccgatttc
     2161 acctcggtgt ttgtcgctgc ggaggaaggc tatttcaaga aacgcaatct cgatgtggaa
     2221 ctcaagttca ttccgttgaa ctccaccatt cctgcagcca tccagtcgga ttcgctgcag
     2281 atgggcgggc ccacaccgtc cgtgtttctg cagtccgtcg atggcggtct ggaccatgtg
     2341 gtgattgctg gcggtggcgt gacgtccaag accatcaccg gctttggtct ggtggcgcgc
     2401 gcgggctcgg gcatcaagac gccacaggac tgcatcggca agaagattgg cgtacccggc
     2461 ctgggtgcct tcctgcacgt gaccttccgc gcctggctga aggacaacaa ggtggaccac
     2521 aaaaaagtca actttgtgga agcctccttc ccgcagcacg gtgacctgct gcgcggcggc
     2581 tcggtcgatg ccgtggtgac cgccgacccg ttcatgggcc gcatcaccga cagcggtgtg
     2641 ggctatgtgg tgtcctacta ctcgacgttc ctgcccgaag gaaacccgac catcatccac
     2701 accgccaagc gggaatgggc cgagaaaaat gcagtggcag cccgtgcgtt ccgcgacgcc
     2761 gtggtcgaag gcgctgcctt catggccaag ccggccaaca acgccaaggt acgcgccgcc
     2821 atcggcaaat acatcaagct gccgcccgaa gtgctggcca aggcgcagat cagcccccct
     2881 ggcccggtgg tgaccgagaa gcagctgacc tactgggtgg gcctgatgaa agaccaggag
     2941 atgctccgga ccgagcccgc agtgtccaga ctgatcttca agtgagccgg cccctgtgag
     3001 tgaatcgccc ctgctgcggt tcgaccaggt gggcatccac ctgggcggcc gcgaaatact
     3061 ctcgcccacc tcgttcgagg tggcacgcgg cgaattcgtc tgcatcattg gcccttccgg
     3121 ttgcggcaag accacgctcc tgcgcgcagc agccggattt cttcagccca gtggcggtgc
     3181 cgcattgcgc cacggcaagc ccatcaccgg cccgtcgcgc gaagtggcgt ttgtgtttca
     3241 ggactatggc cgtgcgctgc tgccctggcg caccgtggcg ggcaacatca gcctggcgct
     3301 tgaagcagcc ggcgtgcctg cgcagcagcg tccggaccgc atcgccaagg tgctcgacac
     3361 cgtgggcttg ggcgcgcacg ccaccaaatt tccggcacag ctctccggcg gcatgcagca
     3421 gcgggtgcag atcgcgcgct gcctcgcgca ggagccggag ctgatgatga tggacgagcc
     3481 ctttggtgcg ctcgatgcca tgacccgtga gagcctgcag gatgaagtgt cgcgcctggt
     3541 gcgcagcacc ggattgaccg tgctgtttgt cacccacgac ctcgaagaag ccatttacct
     3601 cagcgaccgc gtcatcgcgc tgcgcgccaa ccccaccgcg gacaagccca gcctggccgc
     3661 catggtggag gtcaagattg cgcgcccgcg cgaccagttg ctgaccaagg agcaccccga
     3721 attcctgcgc ctgcgtcgcg agctgtacgg atttctggga caccactgat gcgtccctgg
     3781 gtttttcccc tgctgttgct cgcgctgctg gaagctgcag cacgctggct ggtgcccggc
     3841 agcgatgcgc tggcaccgcc ctcccaagcc ctgctggcct tcggcaaggc cctgagcgcc
     3901 agtggtgacg actcggtgct gcaggccacg gccttcaccc tctccagtgc ggcactgggc
     3961 ctgttgctgg gcgccgcgct gggtgtcact ctgggcctgg tgctgggcct gtcgcgccgc
     4021 gccgcggcca tggggttcct cagcatcgag gtgctgcgcc ccgtgccctc ggtggccctg
     4081 attccgctgg cgatgctggt gtttggcttt ggctaccgca tggaaatcag cgtggtggcc
     4141 tttgccacgt tctggcccct gctcatcctg acccaggccg ctgcacgcca ggtggagccg
     4201 cagctgctgg aagtggccca cgcgctgggc ctgagcgcct gggcacgcac cacgcaaatc
     4261 gtgctaccgg ccatggtgcc gcggctgttc attgcgctgc gcctgggcgt gtcgtttgcg
     4321 ttggtcgtcg ctgtcaccgt ggag
//
LOCUS       DAOSJM010000199         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17864_length_4341_cov_3.752450, whole genome shotgun sequence.
ACCESSION   DAOSJM010000199 DAOSJM010000000
VERSION     DAOSJM010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17864_length_4341_cov_3.752450"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PKX82_07980"
     CDS             <1..1077
                     /locus_tag="PKX82_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOF52232.1"
                     /translation="KAVKGRVNQVLTFQGLDRMQTQEAGPGDIVLINGIEDIGIGVTV
                     TDPVNPAPLPMLKVDEPTLTMNFCVNTSPLAGREGKYVTSRQIWDRLQKELQHNVALR
                     VNETDEEGVFEVMGRGELHLTILLENMRREGYEMAVSKPRVMFKEINGEKCEPIELVT
                     ADIEEQHQGGVMQALGERKGELVNMEPDGRGRVRLEYRIPARGLIGFSNEFLNLTRGS
                     GLIANIFDCYEPHKGDIGGRKNGVLISMDAGEIFTYALGKLDDRGRMFVKPNDPVYEG
                     MIVGIHSRDNDLVVNATRTKQLTNFRVSGKEDAIKITPPIECTLEYGVEFIEDDELVE
                     ITPKSIRLRKRYLSESERKRAGRS"
     gene            1154..2023
                     /locus_tag="PKX82_07985"
     CDS             1154..2023
                     /locus_tag="PKX82_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF52233.1"
                     /translation="MLTHTRAVFLMVAVALMWSTAGVVTRHLEFARSFEVTFWRALFT
                     VLTLLVILPFFQGRAVFAKIRHASLPFWLSGVCWSVMFTAYMVALTQTSVGNVLVTMA
                     IGPLLTAVVARIFIGHRVAQRTWIAILVAGVGIVYMFAAQLTEGSILGTLVALCVPVA
                     GAANWTITQNAHDQGQDVDLVPAVLVGAVISTLLTFPLAFPFQASAHDVGLLAMLGAG
                     QLAIPCVLSVLCARILKAPEMSLLALLEVIFGILLAWVGANEVPTQAVLAGGALVIGA
                     LLVNEIIGWRQRA"
     gene            2020..3117
                     /locus_tag="PKX82_07990"
     CDS             2020..3117
                     /locus_tag="PKX82_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOF52234.1"
                     /translation="MSDTVVCEVNGSVGFITLNRAKALNALSLEMVRSITEALLGWKA
                     NSNVHAVAIRGTNKAGNFGGFCAGGDIRFFHQAALAGNPELEDFFTEEYFLNHLIHNY
                     PKPYIAFMDGIVMGGGMGISQGASHRLVTSNTKMAMPETNIGLFPDVGGGYFLSRCPG
                     RTGEWLALTGDVIDGAAAIALGLADRMVDGARLQDAWDALATIDAGNPTQLAQWDATY
                     SIAIDAYFTGARGQIDQYFSLESVTAIVKALEASDDAWALKTAETLRKRSPLMLHVVL
                     EQVRRARAMTLADDLRMERDLVRHCFTTAHLGRRGPSSETVEGIRALAVDKDHSPQWN
                     PARIEDVSAEMVAPFFVSPWPAYAHPLADLA"
     gene            complement(3138..3614)
                     /gene="smpB"
                     /locus_tag="PKX82_07995"
     CDS             complement(3138..3614)
                     /gene="smpB"
                     /locus_tag="PKX82_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOF52235.1"
                     /translation="MAKKKPETSPRIADNKKAAFNYFFEERFEAGIVLEGWEVKSLRE
                     GKVQLTDGYVVIRNGELFIIGLQINPLHTASSHVSPDKVRTKKLLMHKEEIKRLIGKV
                     EQKGYTLVPINLHWKSGKVKCEIALAKGKAEHDKRDTIKDREGKREVERAMKDRNR"
     gene            3704..4147
                     /locus_tag="PKX82_08000"
     CDS             3704..4147
                     /locus_tag="PKX82_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HOF52236.1"
                     /translation="MKTVHKSVLIWYSPQEMYALVTDVNQYPQFLPWCDHANVVEQDT
                     SGMTAEVGIAFAGVRQTFITRNTHTEASRVDMQLVKGPFSKLDGQWHFHPLGDGSQRA
                     CKVELTLNYGFDNATLAKLVGPVFDKIAASMVDAFVKRAQQVYGD"
     gene            4137..>4341
                     /locus_tag="PKX82_08005"
     CDS             4137..>4341
                     /locus_tag="PKX82_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOF52237.1"
                     /translation="MATEANLCITVVYATAPRVIREVEIQLTHGATVADAIRASGVLE
                     GVCADEADALTVGIWGRKAGLTHV"
BASE COUNT          836 a         1226 c         1362 g          917 t
ORIGIN      
        1 aaggcggtca aaggccgcgt caaccaggtg ctgactttcc agggtctcga tcgtatgcag
       61 acgcaggaag ctggtcccgg cgatatcgtg ctgatcaacg gcattgaaga catcggcatc
      121 ggcgtcacag tgactgatcc cgtcaacccc gcaccgctgc caatgctcaa ggtagacgag
      181 cctacgctga ccatgaactt ctgcgtgaac accagcccgc tggctggccg cgaaggcaag
      241 tacgtgacca gccgccagat ctgggaccgc ctgcaaaaag aactgcagca caacgtggcg
      301 ctgcgcgtca acgagactga tgaagaaggc gtgtttgaag tgatgggccg tggtgaactg
      361 cacctgacca tcctgctgga aaacatgcgc cgcgaaggtt acgagatggc tgtttccaag
      421 ccacgcgtga tgttcaagga aatcaatggc gagaagtgcg agcctatcga gctggtcact
      481 gccgacatcg aagaacagca ccagggcggc gtgatgcagg cgctgggcga gcgcaagggc
      541 gagctggtga atatggagcc ggacggccgt ggccgtgttc gcctggagta ccgcattcct
      601 gcgcgtggtc tgattggttt ctccaatgaa ttcctgaacc tgacccgcgg ttctggcctg
      661 attgccaata ttttcgactg ctacgagccg cacaaaggcg acatcggcgg ccgcaagaac
      721 ggcgtgctga tttccatgga tgcgggtgaa attttcactt acgcactggg caaactggac
      781 gaccgcggcc gcatgttcgt gaagcccaac gaccccgtgt acgaaggcat gattgtgggt
      841 attcacagcc gtgacaacga tctggtggtg aacgcaaccc gcaccaagca gctgacaaac
      901 ttccgcgttt ccggcaagga agatgcgatc aagatcacgc cgccgattga atgcacgctg
      961 gagtacggtg tggagttcat tgaggacgac gagctggtcg agatcacacc caagtcgatc
     1021 cgtttgcgca agcgctacct gtctgaaagc gaacgcaagc gcgccggacg tagctgatgg
     1081 cccccacgct ccgccgctgc gcgggtcgct gccccccgag ggggctcatg cgcctagggg
     1141 cggcccggcg gctgtgctga cccacacgcg ggctgtattc ctgatggtgg cggtggccct
     1201 gatgtggtcc accgctggtg tggtcacgcg ccatctggag ttcgcccgca gctttgaagt
     1261 gaccttctgg cgggcgctgt ttaccgtgct caccttgctg gtgatcttgc ccttcttcca
     1321 gggcagggcg gtatttgcca agatccggca cgccagcctt ccgttctggc tctccggtgt
     1381 gtgctggagc gtcatgttca ctgcctacat ggtggcgctc acgcaaacca gcgtgggtaa
     1441 cgtgctcgtc acaatggcga tcgggccttt gctgaccgcc gtggtagccc ggatcttcat
     1501 cggacaccgt gtggcccaac gtacctggat cgccatcctg gtcgcgggcg tgggtatcgt
     1561 ttacatgttt gccgcccagc tgactgaggg gtccatactc ggcaccctgg tggccctgtg
     1621 cgttccggtt gccggggctg ccaattggac aatcacccaa aacgcgcatg accaggggca
     1681 ggatgtggac ctggtacccg cagtactggt aggtgcagtc atctctacgc tgctgacatt
     1741 tcccctggct tttccatttc aggccagtgc acacgatgtc gggctgctgg ccatgctggg
     1801 ggcagggcaa ttggccattc cctgtgtgct cagcgtcctg tgcgcgcgga ttctcaaggc
     1861 cccagagatg tcattgctgg cgctgctgga ggtgatattt ggaatactgc tggcgtgggt
     1921 gggcgccaat gaagtcccga cccaggcagt attggcggga ggcgcgctgg tgattggtgc
     1981 gctattggtc aatgaaatca ttggatggag gcaacgcgca tgagtgatac ggttgtatgt
     2041 gaagtcaatg gaagcgtagg gttcattacc ctgaaccggg ccaaagcgct gaacgcgctg
     2101 agcctggaga tggtgcgcag catcaccgag gccctgctgg gctggaaggc gaattccaac
     2161 gtgcacgccg tggccatccg gggtaccaac aaggccggaa attttggcgg attctgcgcg
     2221 ggtggtgata tccgtttctt ccaccaggcg gcactggccg gcaaccccga actcgaagat
     2281 ttctttaccg aggaatattt cctcaaccac ctgatccaca actaccccaa accctatatc
     2341 gctttcatgg acggtatcgt gatgggcggc ggcatgggta tcagccaggg cgcatcgcat
     2401 cggctggtga ccagcaacac caagatggcg atgccggaga ccaatatcgg cctgtttccg
     2461 gacgtgggcg gcggctattt cctgagccga tgcccgggcc gcaccggtga gtggctggcc
     2521 ctcacgggcg atgtgatcga tggggctgcg gccatcgccc tgggtcttgc ggaccggatg
     2581 gtggacggtg cgcgtctgca ggacgcgtgg gatgcgctgg cgaccatcga cgcgggcaat
     2641 ccgactcaac tggcgcagtg ggatgctaca tattccatag ctattgacgc atatttcacg
     2701 ggggctagag gccagattga ccaatatttc tccctggaat cggtcacagc catcgtcaag
     2761 gcgctggaag catctgacga tgcttgggcg ctcaaaactg ctgaaactct gcgcaagcgc
     2821 tcgccgttga tgctgcatgt ggtgctggag caggtgcgca gggcgcgcgc catgacgctg
     2881 gccgacgatc tgcgtatgga acgtgatctg gtgcgccact gctttacgac cgcccatctg
     2941 ggccgcaggg ggccatcgag cgaaacggta gaaggaatac gggccctggc ggtcgacaaa
     3001 gaccacagtc cccagtggaa tcccgcgcgg attgaggacg tcagtgcgga gatggtggcg
     3061 ccattctttg tcagcccctg gccggcctac gcgcacccgc tggctgacct ggcctgacgc
     3121 cctgatgggt gcccaaccta gcggttgcgg tccttcatgg cgcgctcgac ttcgcgcttg
     3181 ccttcgcggt ctttgatggt gtcgcgtttg tcatgctctg ccttgccttt ggccagggca
     3241 atctcacact tcaccttgcc gcttttccag tgcagattga tgggcacgag cgtgtagccc
     3301 ttttgctcca ctttgccaat gagccgcttg atctcttcct tgtgcatcag cagctttttg
     3361 gtacggacct tgtcgggact gacatggctg gacgcggtgt gcagtgggtt gatctgcagt
     3421 ccgatgatga agagttcacc attgcgaatc acgacgtaac cgtcggtcag ttgcaccttt
     3481 ccctcgcgca gggatttgac ctcccagccc tccagcacga tgcccgcctc gaagcgctcc
     3541 tcaaaaaagt agttgaacgc ggctttcttg ttatcggcga tgcggggcga tgtttcgggt
     3601 ttcttctttg ccatgggtgt gagatgggga tgacagacag gattgaaagg gctggtgaac
     3661 acgcatcagg ccttgcttac aatcccgcgc agacctccat tctatgaaaa ccgtccacaa
     3721 gtccgttctg atctggtaca gcccacagga aatgtatgcg ttggtgaccg atgtcaacca
     3781 gtatccccag ttccttccct ggtgcgacca cgccaatgtg gtcgagcagg atacgtctgg
     3841 catgactgca gaagtgggca ttgcatttgc cggcgtgcgc cagaccttta tcacccgcaa
     3901 tacacataca gaagccagcc gtgttgacat gcaactcgtg aaagggcctt tctccaagct
     3961 ggatggccag tggcactttc acccactagg agatggctcg caacgtgcct gcaaggtgga
     4021 gcttacgctg aactatggct tcgacaacgc cacgctcgcc aagctggtgg ggccggtttt
     4081 tgacaagatc gcggcaagca tggtggatgc ctttgtcaag cgggctcagc aggtttatgg
     4141 cgactgaggc aaacctttgc attacggtgg tgtatgcaac ggcaccacgc gtgatcaggg
     4201 aagtcgaaat tcagctgacc cacggggcga cggttgctga cgcgatccgg gccagtggcg
     4261 tgttggaggg ggtgtgtgcc gacgaggccg atgccctgac ggtgggaatc tggggacgta
     4321 aggcgggctt gacgcatgtg c
//
LOCUS       DAOSJM010000200         4334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17898_length_4334_cov_4.583314, whole genome shotgun sequence.
ACCESSION   DAOSJM010000200 DAOSJM010000000
VERSION     DAOSJM010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17898_length_4334_cov_4.583314"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PKX82_08010"
     CDS             complement(<1..999)
                     /locus_tag="PKX82_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOF52238.1"
                     /translation="MDRTLSAPSRRVAALLIAAPASGQGKTTITSALARLHTRQGRKV
                     RVFKCGPDFLDPYWHSLASGAPVYQMDLWMTGEMDCRARLHAAAQEADLIIVEGVMGL
                     FDGEPSAADLAQRFGLHVLAVVDASAMAGTFGALAFGLQHYRPDMPWAGVLANRVASE
                     RHAGMLARSVRDPAHFMGAVMRNAAMTLPERHLGLTVASEVTDAMERLDAAADALATT
                     PLGQMTLEELQRWAVDFVAPEAEGGWAGCSAPCPPPALRAPPLPAQNSLPTRFPTLQG
                     KTIAVARDAAFCFIYAANLDTLRAIGAELVFFSPLTDATLPACDALWIPGGYPELHA"
     gene            complement(984..2840)
                     /locus_tag="PKX82_08015"
     CDS             complement(984..2840)
                     /locus_tag="PKX82_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOF52239.1"
                     /translation="MRKRAPVRLCASALAVFAAFPALAQSQAVATLAEVVVTATRVEQ
                     SLTHVLADVTVIDADVIERSGAVSLADVLVHAPGIEMVRNGGPLGTTSLYLRGGNTNH
                     TVVMIDGIRLDTQNGSGGATCQSIPAQQIERIEILRGPAAAIYGSDAIAGVIQVFTKN
                     GQSGIQPSVGLGLGTYGTRTATAGLLGAHEGWRYALSLGRETSDGYNIQPAANPDNDG
                     YTRETATLRLGRNLVAGHKLEGTWVYTDTDAGYDSTTTKSNINYAKDNRTVQKLNALG
                     LNWNAKWSDVWSSTVGVTQALDHYQTLPSPGAVPSYDTETTIQTALWNNTLRWRDAIW
                     TVGLEGRRDALTNSGTTPKNTKRNQDALALGYGSQMDAHSWQANVRLDHDSDFGDHTT
                     ETLAYGYKWSPQWRLSASMGTGFRSPTLYQRFSDYGFAGLKPEESFNRELGVTYRYET
                     QTLSVVAYNNQITNLIAFGAAGACVSSFGCYANTAKATLQGVTLSGESRWGSTVWSAS
                     LDLQDPVDADNGNRLARRAQTVLKLGAETSWQGWNWKADVLLSGDRFDDAPNKTLLPG
                     YGLLNVGASRSIAPQTELLVRLDNLGDKAYETAKGYANPGRTLFVGMKWTGL"
     regulatory      complement(2933..3169)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3206..3430
                     /locus_tag="PKX82_08020"
     CDS             3206..3430
                     /locus_tag="PKX82_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HOF52240.1"
                     /translation="MSNQTQIDQIAERVERLLVRHAELQRTNALLSSQVEALTQERDS
                     LKSRLGAARARVDALLERLPDPTAAHKEPS"
     gene            3427..3768
                     /locus_tag="PKX82_08025"
     CDS             3427..3768
                     /locus_tag="PKX82_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOF52241.1"
                     /translation="MKQLEVQIMGQSYLLGCPEGGDTRLLEAVERVDTAMCKIRDAGK
                     VRARDRIAVLAALNLAFDLSDRPAVAVAASAAAPAGAAIATEPVGDPDLLLTSLLERL
                     DEALGEDGRLL"
     gene            4021..>4334
                     /locus_tag="PKX82_08030"
     CDS             4021..>4334
                     /locus_tag="PKX82_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOF52242.1"
                     /translation="MLDPVLILELAALGLCTGYLAGLLGIGGGMLIGPFMTLIMSTQG
                     VSADLAVKMAIATSMATIIFTSVSSVRAHHKRGAVRWDIVKGLAPGIVLGGAIGSMGI
                     FAV"
BASE COUNT          838 a         1415 c         1264 g          817 t
ORIGIN      
        1 cgcatgcagt tccgggtagc cgccaggaat ccacagtgca tcacacgcgg gcaaggtcgc
       61 gtcagtcaat ggcgagaaaa acaccagctc tgcaccgatc gcacgcaggg tatcgaggtt
      121 ggccgcgtag ataaagcaaa acgccgcatc ccgcgccacc gcaatcgttt tgccctgcaa
      181 cgtaggaaag cgggtgggca ggctgttctg cgcaggtaaa ggaggagccc gcaacgcggg
      241 cgggggacac ggagcagaac agcctgccca accgccttcc gcctcaggcg caacaaagtc
      301 cacagcccag cgctgcaact cttccagcgt catctgcccc agcggcgtag tggccaaagc
      361 atcggccgcc gcatccagcc gctccatcgc gtccgtcact tcgctggcca ccgtcaggcc
      421 cagatgccgc tctggcaggg tcatggcggc gttgcgcatc acagcaccca tgaaatgcgc
      481 cgggtcgcgc acgctgcgcg ccagcatgcc ggcatggcgc tcgctggcca cgcggttggc
      541 caacacaccg gcccagggca tgtccggccg gtaatgctgc aaaccaaagg ccagtgcgcc
      601 aaacgtgccc gccatggcgg acgcgtccac caccgccagc acatgcaggc caaaacgctg
      661 ggccaggtcg gctgcgctgg gctcaccgtc aaacaggccc atgacccctt ccacaatgat
      721 caggtcggcc tcctgcgcag cagcatgcag gcgtgcgcgg cagtccatct cgcccgtcat
      781 ccacaggtcc atctggtaca ccggcgcgcc actggccagg ctgtgccagt acgggtccag
      841 aaaatccggc ccgcacttga acacccgcac cttgcgcccc tggcgcgtgt gcaggcgcgc
      901 cagcgcggag gtgatggtgg tcttgccctg cccggaggcg ggcgcggcga tcaggagtgc
      961 ggcgaccctt cgtgagggcg cgctcaaagt ccggtccatt tcattcctac aaacagggtg
     1021 cgtccagggt tggcatagcc cttggcggtt tcgtaagcct tgtcgcccag attgtccaac
     1081 cggaccagca gctcggtttg tggcgcgatg gaccggctcg cccccacatt cagcaggcca
     1141 taccctggca acagggtttt atttggcgca tcgtcaaagc gatcacccga cagcaacacg
     1201 tcggctttcc agttccagcc ctgccatgag gtctcagcac ccagcttcaa caccgtctga
     1261 gcccggcgag ccaagcggtt accgttgtcg gcatcgaccg gatcctgcaa atccagggat
     1321 gcactccaaa cggtagaacc ccatcggctc tcgccgctga gcgtcacccc ttgcaacgtg
     1381 gctttggccg tgttggcata acaaccaaac gaactaacac atgcacccgc agcaccaaag
     1441 gcaatcaaat tggttatctg gttgttgtaa gccaccaccg acaaggtctg tgtctcataa
     1501 cgataggtca cccccagttc ccggttaaag gattcctcag gcttcaaacc ggcgaaacca
     1561 tagtcactga accgctggta cagggtcgga gagcggaagc ccgtgcccat cgatgccgac
     1621 agccgccact gcggcgacca tttgtagcca taggccaggg tctcggtcgt gtggtcgcca
     1681 aaatcactgt catgatcaag gcggacattg gcttgccagc tgtgggcatc catctggctg
     1741 ccgtagccca gtgccagggc gtcctgattg cgcttcgtgt ttttgggggt agtgcctgag
     1801 ttggtcaagg catcgcggcg cccctccagc cccaccgtcc agatggcatc gcgccagcgc
     1861 aacgtgttgt tccacaacgc ggtttggatg gtggtttcgg tgtcatagct tggcacggcc
     1921 ccaggactgg gcaaggtctg atagtgatcc agtgcctgcg tcacgccaac cgtcgaagac
     1981 cagacatccg accacttggc attccagttc agccccaacg cattgagttt ttgtacggtg
     2041 cggttgtctt tggcgtaatt gatattgctc ttggttgtgg tgctgtcgta accagcatcc
     2101 gtatcggtgt acacccaggt accctccagc ttgtgcccgg cgaccagatt gcgcccgagg
     2161 cgaagagttg cggtttctct tgtatagcca tcgttgtcgg ggttggctgc tggctgtatg
     2221 ttgtagccat cgctggtctc gcgtcccaat gacaacgcgt acctccatcc ttcatgcgcc
     2281 cccaacaaac ctgcggtggc ggtacgggtg ccgtaggtcc ccaagcccaa acccacactg
     2341 ggctgaatcc cagactgacc attcttcgta aacacctgga tcacacccgc gatggcgtcc
     2401 gagccataaa ttgctgcggc ggggccgcgc aggatttcga tgcgctcgat ctgctgggca
     2461 ggaatcgact ggcaggtcgc cccgccactg ccgttctggg tatcgaggcg gatgccgtcg
     2521 atcatgacca cagtgtgatt cgtgttgcca cctcgaagat acaggctggt tgtccccagg
     2581 ggaccaccat tgcgcaccat ttcaatgcca ggcgcatgca ccaacacatc cgccagcgaa
     2641 actgccccac tgcgttcgat gacatcggca tcgatcaccg tcacatcggc cagcacatgg
     2701 gtcagcgact gctcgacccg cgtcgccgtc accaccacct ccgccaaagt tgctacagcc
     2761 tggctttgcg ccagagccgg aaaagcggca aacacggcca aagcagacgc gcacaggcgc
     2821 acgggtgcgc gtttgcgcat tgaaaaagca aaagatttca tagtaggaaa agaaccaaca
     2881 aattaccctc actgtcttcc ccgacagtgc atgggcgatt ggccgcacgc aagcgtgcaa
     2941 ccaccgtatt ggccggtatc cgggctggtg gatgcaacct ccatcgcctt cccaagcgcg
     3001 tgttcagaac gctcagtggc cttgatggaa gcggagcaca ggggctcgtc cacttaccgt
     3061 tgcgggggca gcgcaggtta ggttggctgg tgacagcgtc tccctcctgc ttcccgttga
     3121 actgcggtgt gtgaaccaca ccgcgagcac caacccgcgc attttacgtt gtccgggctt
     3181 gcgtaaaccc tacaatgcag gatctatgtc gaaccagacc caaatcgacc aaatcgccga
     3241 gcgcgtggag cgcctgctgg tgcgccatgc cgagttgcag cgcaccaacg cgctcctgag
     3301 cagccaggtc gaggcactca cccaggagcg tgactccctg aaatcccggt tgggggccgc
     3361 acgggcacgc gtggatgcct tgctggaacg gctacccgac cccaccgctg cacacaaaga
     3421 accatcatga aacagcttga agttcagatc atgggccaga gctacctgct tggctgtcca
     3481 gagggtggtg atacccgttt gctggaagcc gtggagcgcg tggacaccgc catgtgcaag
     3541 attcgcgatg cgggcaaggt acgtgcacgc gaccgcattg cggtgctggc cgcactgaac
     3601 ctggcttttg acctgtccga tcggcctgcc gtggcagtcg ccgccagtgc tgcggcaccc
     3661 gccggtgcgg ccatcgccac agagccggtg ggcgatcccg acctgctgct gacctcgctg
     3721 ctggagcgtc tggacgaggc tctgggcgaa gacggccggc tgctttaatc tatcccgcag
     3781 caacccgcgc agcggtggag cgtgggggct acaatccttc tgtctgcagt gcatgccggg
     3841 ctttatattt ccttgaacca atgctcttag agcccgggcc tggaacattg tcctgccagc
     3901 gtgatcatct cgcgtcagat gaacccaagg cttgcactga cctagcccac ctgaaccccg
     3961 gttcaggatg ccggtccggt ggcactgcag acaccttatt ctccccacac cctctccccc
     4021 atgcttgatc ccgtcctgat cctcgaactt gcggccctgg gcctgtgcac cggttacctt
     4081 gcaggcctgc tgggcattgg cgggggcatg ctgatcggcc cattcatgac gctgatcatg
     4141 tcgacacagg gcgtgtcagc cgatctggca gtcaagatgg cgattgccac ctccatggcc
     4201 accatcatct tcacctcggt ttccagcgtg cgcgcccacc acaagcgcgg cgcggtgcgc
     4261 tgggacattg tcaagggact ggcgcccggc atcgtcctgg gcggcgccat cggcagcatg
     4321 ggtatctttg cggt
//
LOCUS       DAOSJM010000201         4333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17901_length_4333_cov_4.177653, whole genome shotgun sequence.
ACCESSION   DAOSJM010000201 DAOSJM010000000
VERSION     DAOSJM010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17901_length_4333_cov_4.177653"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="PKX82_08035"
     CDS             complement(<1..753)
                     /locus_tag="PKX82_08035"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOF52243.1"
                     /translation="MKLHPTVETVTQRIRARSEGTRQAYLAAINVAAQRKPGADRMGC
                     ANVAHAFAAMPDADKARVTGLDKVKVVAEKGPNIGIVTAYNDLLSAHAPLQHYPDLIK
                     TEARQHGATAQVAGGVPAMCDGVTQGTAGMELSLFSRDTIAMSTAISLSHDVFDAALM
                     LGVCDKIVPGLLIGALHFGHLPTVFVPAGPMTSGLSNNEKSKVREQAAQGLVGRTELL
                     RAESAAYHGQGTCTFYGTANSNQMLLEAMGLHV"
     gene            924..1400
                     /locus_tag="PKX82_08040"
     CDS             924..1400
                     /locus_tag="PKX82_08040"
                     /inference="COORDINATES: protein motif:HMM:NF022766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HOF52244.1"
                     /translation="MKLAIVTLMVALSGGMAFGQTPQGRSLGQNLYLPIYSHIWHGEM
                     DKNGQPMKTLVSVSVSIRNTDPAKSIRIVSAQYFDTDGKKLRDYVTAPKTIGPMGTYE
                     LFVPRSDDTGGSGANFVITWKSDKPASPPIVEGFHANLPVGRSIAFTSSARALPEE"
     gene            complement(1428..3125)
                     /locus_tag="PKX82_08045"
     CDS             complement(1428..3125)
                     /locus_tag="PKX82_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HOF52245.1"
                     /translation="MLTILLLLTAAVAAVALARRLKLPSMLAYLAIGIALGPHGLALL
                     SESEAIAELAEFGIVFLMFSIGLEFSIKRLHAMRKLVFGFGASQMVVTALCTGLVTWI
                     GYGQGWKSGVAIGLAVAMSSTAIVARMLSDSFELHSRSGRQTMGVLLFQDLAVVPCLI
                     LLPALAAKDDHLLSSLAIAAVQAAVVLTLLVWLGQRYLGRVIHAIALHRSDELLVLST
                     LWIVVGLSYLTSMSGLSMALGAFVGGMLISETIFRHQVEADIRPFRDILLGLFFVTVG
                     MMLDLRYVLANPHLLLLAVLLLIGGKAAVMLVVARVARTPWVVSLRTAAQLAQAGEFG
                     LVLVELGYQLKLITLDVFQITLPAMLISMFVAPFLIARAAKLSVDLGRGDWAHSVTAI
                     QNVAVHSMDLQNHVLICGFGRVGGRIGEFLTLEDIPFIALDVDPQRIARANAVGLNVV
                     FGNADRAEVLKAAGVSRARAVVVSYPDVHSAERVVRVVRASRPNVPIVVRTSDAKDVG
                     RLKAAGATEVIPEVQESGLLVAAETLVQAGVPMEKALIHVRSARAARYQSLKDFYGK"
     gene            complement(3252..3725)
                     /locus_tag="PKX82_08050"
     CDS             complement(3252..3725)
                     /locus_tag="PKX82_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOF52246.1"
                     /translation="MSSQDDDNIGVVLGVIFGVIALVIALVVGISQYSVNQFAAKPAA
                     AAAADGASVKVEGGVVKFYFASGKADLAAGGNEALTDIVHAVHAGKKILISGFHDATG
                     DATANAELAKQRALKVRDALAALGVAQDQVELKKPEQTLATGSDAEARRVEVSVQ"
     gene            3846..>4333
                     /gene="pdxH"
                     /locus_tag="PKX82_08055"
     CDS             3846..>4333
                     /gene="pdxH"
                     /locus_tag="PKX82_08055"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOF52247.1"
                     /translation="MTSIADLRKSYERAELDEGASHPDPLNQFDQWIKEAIHSEVPEP
                     NAMTVATVGSDLRPSTRVVLIKGYDAQGIVWYTNYESRKGRELAGNPFAALQFHWVEL
                     ERVVRIEGRVEKVSAEESDAYFQSRPLDSRVGAWASPQSQVIPGRTVLVTNAAKYAAQ
                     FLL"
BASE COUNT          957 a         1400 c         1238 g          738 t
ORIGIN      
        1 cacatgcagg cccatggctt cgagcagcat ctggttactg ttggcggtgc catagaaagt
       61 gcaggtgccc tgcccgtggt aggccgccga ctcggctcgc aatagctcgg tgcgccccac
      121 caggccctgc gccgcctgct cgcgcacctt ggacttttcg ttgttcgaca gccccgacgt
      181 cataggaccc gccggcacaa acacggtcgg caggtgtccg aagtgcaagg cgccaatcag
      241 cagtcccggg acaatcttgt cgcagacacc cagcatcagt gctgcatcaa acacatcgtg
      301 gctcagggaa atggcggtgg acatggcaat cgtgtcgcgg ctgaacaggc tcagttccat
      361 gcccgccgtg ccctgtgtca caccatcgca catggccggt acgccacccg ccacctgggc
      421 cgtggcgcca tgctggcggg cctcggtctt gatgaggtcg gggtaatgct gcagaggcgc
      481 gtgggccgag agcagatcgt tgtacgcggt gacgatgccg atgttggggc ccttctcggc
      541 aacgaccttg actttgtcca gccccgtgac gcgtgccttg tcggcgtccg gcatggcggc
      601 aaaggcatgc gccacattgg cacaccccat gcggtccgcc ccaggcttgc gctgtgctgc
      661 gacattgatc gccgccagat acgcttgccg cgttccctcg ctgcgcgcgc ggatgcgttg
      721 tgtcaccgtt tcaactgtgg gatggagttt cataaaattt ggtaactaaa ttacatcctg
      781 aatggtactc ctgaatgggc attcaggcac gtgtgggggt taccagatgg ttaccaacac
      841 agacgccacg gcggtccgga acaggccttg cgccaaccga agtaacatca cccggtgacc
      901 tgcaattcgc agcggagatg tctatgaaac tggcaatcgt gaccctgatg gtggccctgt
      961 ccggcgggat ggcgttcggc cagactccgc agggcaggtc tctgggccag aacctgtacc
     1021 tgccgatcta ctcgcacatc tggcatggcg agatggacaa gaacgggcag ccgatgaaaa
     1081 ccctggtttc ggtctcggtg agcatccgca atacggaccc cgcgaaatcc atccgcatcg
     1141 tttctgcgca gtatttcgac accgatggca aaaaactccg ggactatgtg actgcaccca
     1201 aaaccattgg ccccatgggc acctatgagc tgtttgttcc gcgaagcgac gataccggag
     1261 ggtccggcgc caatttcgtg atcacctgga aatcggacaa acccgccagc ccgccgatcg
     1321 tcgagggctt ccacgccaat ctgccagtgg gcagatccat tgcgttcacc agctcggcgc
     1381 gcgctttgcc ggaggagtga gggccacggg tctccgcggc ccggacctta tttcccgtaa
     1441 aagtctttga gggactggta gcgggccgcc ctggccgagc gcacatggat cagcgctttc
     1501 tccatgggaa cacccgcctg caccagggtt tcggccgcaa cgagcaggcc actttcctgc
     1561 acttcgggaa tgacctcggt agcacctgca gctttcagtc ggcccacatc cttggcatca
     1621 ctcgtccgca caacgatggg gacattcggg cggcttgcac gcaccacgcg gaccacccgc
     1681 tcggccgagt gcacatccgg gtagctgacc accacggcac gcgcacgcga gacacctgcc
     1741 gctttcagaa cctcggcgcg gtctgcattg ccgaacacca cattgagtcc cactgcattg
     1801 gcccgcgcaa tgcgctgggg atcgacatcc agggcaataa agggaatgtc ttccagcgtc
     1861 agaaactccc cgatacgccc gccgactcga ccaaagccgc aaatcagcac atggttctgc
     1921 agatccatgc tgtgcacagc gacattctga atcgccgtca cgctgtgtgc ccagtcccca
     1981 cgccccaaat cgaccgacag cttggccgcc cgggctatga ggaagggcgc cacaaacatc
     2041 gaaatcagca tggccggcag cgtgatctgg aagacatcga gcgtgatcag ctttaactgg
     2101 tagcccagtt ccaccagcac cagcccgaac tcacccgcct gggccagctg cgcagcggta
     2161 cgcaaggaca ccacccaggg cgtcctggcc acacgcgcca ccaccagcat gacggccgcc
     2221 ttcccgccaa tcagcagcaa aaccgccagc aggagcaggt gcgggttggc cagcacatag
     2281 cgcaggtcca gcatcatgcc cacggtgacg aagaacaacc ccagcaaaat gtcccggaac
     2341 ggacggatat cggcttccac ctggtggcga aaaatagtct ccgatatcag catgcccccc
     2401 acaaaggcac ccagtgccat ggacaggccg ctcatggacg tgaggtacga caggcccacc
     2461 acgatccaca acgtcgagag gaccagcaac tcatcggacc ggtgcagggc gatcgcatgg
     2521 atgacacggc caaggtacct ctgccccagc cagaccagca gggtgagcac gacggctgcc
     2581 tgcaccgctg cgatcgccag cgacgaaagc aaatggtcat ctttggccgc cagtgcgggc
     2641 aacagaatca ggcagggcac caccgcaaga tcctgaaaca acagcacccc catggtctgg
     2701 cggcccgagc gcgagtgcag ttcaaaactg tccgagagca tgcgggcgac gatggccgtc
     2761 gacgacatgg ccacggccag cccgatggca acgccacttt tccagccctg cccatagcca
     2821 atccaggtca ccaggccagt gcacaacgca gtcaccacca tctgcgaagc accaaaaccg
     2881 aataccagct tgcgcatggc atggagacgc ttgatgctga actccagacc gatggagaac
     2941 atcaaaaaga caatgccgaa ttctgcgagt tctgcaatgg cttcggactc tgacagcagc
     3001 gccaggccat gcggtccaag cgcaatgccg atcgccaggt aagccagcat gctcggcagt
     3061 ttgagcctgc gtgccaacgc gacagcagca accgcggcgg tcagtaacag gagaatggtg
     3121 agcaagttat cgtgcacgcc ggaatcatag ctatcgagcc aaacggatcg cccttgcgga
     3181 cgacgcaaaa acaaagggcc cgatcgtgtc gggcccttgg tgctggtata ccggccaggc
     3241 cggtggtgga gttactgtac cgaaacttcc acccggcggg cttcggcatc agaaccggtt
     3301 gccagggttt gctcaggctt cttcaattcc acctggtcct gggccacgcc cagggcagcc
     3361 agggcgtcac gcaccttgag tgcgcgctgc ttggccagtt cggcattggc agtggcgtca
     3421 ccggtcgcgt catggaagcc actgatcaga atcttcttgc ccgcatggac agcgtgcacg
     3481 atgtcagtca gggcttcatt gccaccagcg gccagatccg ccttgccaga agcaaaatag
     3541 aacttcacca cgccgccttc cactttcaca ctggcgccat cggctgcagc agccgcagcg
     3601 ggtttggctg cgaactggtt caccgaatac tggctgatgc ccaccacgag ggcaatcacc
     3661 aaggcaatca cgccaaaaat cacacctaga acaacaccaa tgttgtcgtc atcctgagaa
     3721 gacatcgctt gaactcccta aaaaatagaa cctgaattgt aaagcgacac aggtgcagtc
     3781 accgtccgtc aaatcgacaa gcgccaaagg cagtttctcc cgagagacat gcctatactt
     3841 gccccatgac ctccatcgca gaccttcgca agagctacga acgcgccgaa ctggacgaag
     3901 gcgcctcgca ccccgacccc ctgaaccagt ttgaccagtg gatcaaggaa gccatccaca
     3961 gcgaggtacc cgaacccaac gccatgaccg tggccacggt tggcagcgac ttgcgcccct
     4021 ccacccgtgt ggtgctgatc aagggctacg acgcgcaggg catcgtctgg tacaccaact
     4081 acgagagccg caagggccgc gaactggccg gcaacccgtt tgccgcactg caattccatt
     4141 gggtggagct ggagcgggtg gtccggattg aaggcagggt ggaaaaggtc agcgcagaag
     4201 aaagcgacgc ctatttccag agccggccgc tggattcacg ggtgggtgcc tgggcttcgc
     4261 cgcaaagcca ggtcattccg ggcagaaccg tgttggtaac caacgccgcc aaatacgccg
     4321 cacagtttct gct
//
LOCUS       DAOSJM010000202         4330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17916_length_4330_cov_4.402339, whole genome shotgun sequence.
ACCESSION   DAOSJM010000202 DAOSJM010000000
VERSION     DAOSJM010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17916_length_4330_cov_4.402339"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /gene="rpoD"
                     /locus_tag="PKX82_08060"
     CDS             <1..1595
                     /gene="rpoD"
                     /locus_tag="PKX82_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOF52248.1"
                     /translation="VDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEGGLMAM
                     MEAISASPATIAEILRLGEEIREGKVVITTIVDGFSNPNEADDYVAEEDFDEFDEADD
                     DDGKGGSKALTKKLEELKKQALERFDKLRELFEKVHKVYDKEGYGTPNYTKAQKALSE
                     ELMTIRFTAKAIEKLCDMVRAQVDDVRKKERELRRIIVDKCGYPQESFIADFSGRDKN
                     GNKVASQLLNLKWIEKQAASGKPWAAVMGRNIPPVQDLQQKLSDLQARVVVPLDQLKD
                     INKRMNEGESSSRAAKKEMIEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDK
                     FEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQEFGF
                     EPDASVLAEKMEIPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDSANTAPMEA
                     AMQAGLRDVVKDILDSLTPREAKVLRMRFGIEMTSDHTLEEVGKQFDVTRERIRQIEA
                     KALRKLKHPSRSDKLRSFTDTL"
     gene            complement(1671..1952)
                     /locus_tag="PKX82_08065"
     CDS             complement(1671..1952)
                     /locus_tag="PKX82_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HOF52249.1"
                     /translation="MEFNFEQVHNYYERLVFEEVARRSHSYPEFTSDMLADVACVALN
                     RLPARYVRHDVDMMFYLTEQERHAIELSLEEGLQFAFRFVRDRATKSRI"
     gene            2229..2540
                     /locus_tag="PKX82_08070"
     CDS             2229..2540
                     /locus_tag="PKX82_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOF52250.1"
                     /translation="MSSKETAAIGQLLGLLESRYAMRVLWALRDGHAQTFRLLQDSVG
                     GITPNTLNTRIKELREAGLLTHGSDGYCVTTAGADLLKRLGDLQAFATKWSASQAKKV
                     K"
     gene            2692..3045
                     /locus_tag="PKX82_08075"
     CDS             2692..3045
                     /locus_tag="PKX82_08075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF52251.1"
                     /translation="MQTTASGLQYLDTVEGTGPVATKGQQVTVHYTGWLYNDGAQGAK
                     FDSSKDRNDPFVFNLGAGMVIRGWDEGVAGMKVGGARTLIIPAALGYGARGAGGVIPP
                     NATLKFDVELLGVAG"
     gene            3152..3715
                     /gene="grpE"
                     /locus_tag="PKX82_08080"
     CDS             3152..3715
                     /gene="grpE"
                     /locus_tag="PKX82_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOF52252.1"
                     /translation="MSDNQSNPQTPAGNAGFQSTEDMVAAMASHEADALPKVQAELAA
                     LQAKSAELADQYLRAKAEAENARRRAEDEISKARKFAVEAFAESLLPVADSLEAGLII
                     KDATAEQIREGAQATLRQLLAALERNKVLAINPSAGTKFDPHQHQAISVVPSEQEANT
                     VVSVLQKGYSIADRVLRPALVTVSAPK"
     gene            3835..>4330
                     /locus_tag="PKX82_08085"
     CDS             3835..>4330
                     /locus_tag="PKX82_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOF52253.1"
                     /translation="MGRIIGIDLGTTNSCVAIMEGNTPKVIENAEGARTTPSIIAYQE
                     DGEVLVGASAKRQSVTNPKNTLYAVKRLIGRKFSEKEVQKDIDLMPYKIAAADNGDAW
                     VEVRGNRMAPQQVSADILRKMKKTAEDYLGEEVTDAVITVPAYFNDAQRQATKDAGRI
                     AGLDV"
BASE COUNT         1050 a         1319 c         1225 g          736 t
ORIGIN      
        1 ccgtggacag cgaattcggc cgcaccaccg acccggtgcg catgtacatg cgcgaaatgg
       61 gcacggtgga gctgctgacc cgcgaaggcg agattgagat cgccaagcgc atcgagggcg
      121 gcctgatggc catgatggaa gcgatttccg catcccccgc caccattgcc gagatcctgc
      181 gcctgggcga ggaaatccgc gaaggcaagg ttgtcatcac caccatcgtg gacggcttct
      241 ccaaccccaa cgaggccgac gactatgtgg ccgaagaaga ctttgacgag ttcgatgaag
      301 ccgacgatga cgacggcaag ggcggctcca aggctctgac caagaagctc gaagagctga
      361 aaaagcaggc gctggagcgc tttgacaagc tgcgtgagct gttcgaaaaa gttcacaagg
      421 tctacgacaa ggaaggctac ggtacgccca actacaccaa ggcacaaaaa gccctgagtg
      481 aagagctgat gaccatccgc tttaccgcca aggccatcga gaagctgtgc gacatggtgc
      541 gcgcccaggt ggacgatgtg cgcaagaagg agcgtgaact gcgccgtatc atcgtcgaca
      601 agtgcggcta cccacaggag agcttcattg ccgacttcag cggccgcgac aagaacggca
      661 acaaggtggc cagccagctg ctcaacctga agtggatcga gaagcaggct gcatccggca
      721 agccctgggc ggcggtgatg ggccgcaaca ttccgccggt gcaggatctg cagcaaaagc
      781 tgtccgacct gcaggcccgc gtggtggtgc cactggacca gttgaaagac atcaacaagc
      841 gcatgaacga aggcgaatcg tcttcacgtg cggccaagaa ggagatgatc gaggccaacc
      901 tgcgcctggt gatctcgatt gccaagaagt acaccaaccg cggcctgcag ttcctggacc
      961 tgatccagga gggcaacatc ggcttgatga aggcggtcga caagttcgaa taccgccgcg
     1021 gctacaagtt ctcgacgtat gcgacctggt ggatccgcca ggccatcacc cgctccatcg
     1081 cggaccaggc acgcaccatc cgtattccgg tgcacatgat cgaaaccatc aacaagatga
     1141 accggatcag ccgtcagcac ctgcaggagt tcggtttcga gcccgatgca tcggtgttgg
     1201 ccgaaaagat ggaaattccg gaagacaaga tccgcaagat catgaagatc gccaaagagc
     1261 cgatctccat ggaaacaccg atcggtgacg atgacgacag ccacctgggc gacttcatcg
     1321 aagacagcgc caacaccgcg cccatggaag ccgcgatgca ggccggcctg cgcgatgtgg
     1381 tcaaggacat cctggacagc ctcaccccgc gcgaagccaa ggtgctacgc atgcgtttcg
     1441 gtatcgagat gacctccgac cacacgctgg aagaggtggg caagcagttc gacgtgaccc
     1501 gcgagcgtat ccgccagatc gaagccaagg cgctgcgcaa actcaagcac cccagccgct
     1561 cggacaagct gcgcagcttc accgacacgc tgtaagcaca gcgcgcaaaa aaccaaggcg
     1621 cgtggtacct gctccagcag gcaccacgcg cctttttgtt attcagcgaa tcagatgcgc
     1681 gacttggtcg cccggtcccg cacaaacctg aacgcaaact gcagcccttc ttccagcgac
     1741 agctcaatgg catggcgctc ctgctcggtc aggtagaaca tcatgtccac gtcatgtcgc
     1801 acgtaccggg cgggcaaacg gttcaaggcg acacaggcaa cgtccgccag catgtcgctg
     1861 gtgaattcgg gataggaatg actgcgccgc gccacctcct cgaacacgag tcgctcgtaa
     1921 tagttgtgaa cctgctcaaa attgaactcc atgcattcct ccattgcaca cgcacctgcg
     1981 cgcggcgatt gcggctacac cttagcatca ggccgtccca ctgacaagtg gagcaccaag
     2041 tgcccatgat cggcgaaatt gtcccccaat ttgtgcatcg gcgacacact gacccccaca
     2101 ccagccccat ttgtaagctt gtgcaagcta tgaaacttgt agcattgcga aaactgcgat
     2161 atagtttccg tccattgatc gtgccgccag cctgtccggc cgatcccgaa ttgaggagag
     2221 gtaaattaat gagctctaaa gaaaccgccg ccattggtca actgctgggt ttgctggaat
     2281 cccgctatgc catgcgcgtg ctgtgggccc tgcgtgacgg acatgcacag acgtttcgtc
     2341 ttctgcagga cagcgtaggc ggcatcaccc cgaacacact gaacacccgt atcaaagagc
     2401 tgcgcgaagc aggattgctg acgcacggga gtgacggcta ttgcgtgacg acggccggtg
     2461 cagacctgct caaacgcctg ggcgacttgc aggcctttgc gaccaagtgg tccgccagcc
     2521 aggccaagaa agttaaatag gcactttcat cgggattcac atcccgaagc cacccgcaag
     2581 acaggcacag cctgtctttt ttttgcctgc ggcataatta cgggttttgg gtcaacagcc
     2641 cccgcagtac gggcacgatc cgctacaaca ttcatagcaa aaggacattc catgcaaacc
     2701 accgcaagcg gcctccagta cctcgacacc gtggaaggca caggccccgt agccaccaag
     2761 gggcagcaag tcacggtgca ctacaccgga tggctctaca acgacggcgc ccagggtgcc
     2821 aagttcgact ccagcaagga ccgcaacgat ccctttgtgt tcaatctggg ggccggcatg
     2881 gtgatccgtg gctgggacga aggtgtggca ggcatgaagg tcggtggagc ccgtacgctc
     2941 attattcctg ccgccctggg atacggcgcg cgcggtgcgg gtggtgtcat tccccccaat
     3001 gccacgctga agttcgacgt tgagctgctg ggtgtagcgg gctgatcttt caccgctgct
     3061 accaggccca gccccacaac ggtgtgggcc ttgaatttgt tgccagcggc cccagatgga
     3121 ggccagcaac tgaaaccccg ggtacatccg aatgtctgac aaccaatcca atccacaaac
     3181 acctgctggc aacgccggct ttcagagcac cgaagatatg gtcgccgcca tggcgtcaca
     3241 cgaagcagac gcattgccca aagtccaggc cgaactcgct gcactgcaag ccaagagtgc
     3301 cgaactcgca gatcagtacc ttcgcgccaa agcggaagca gaaaacgccc gccgccgtgc
     3361 cgaagacgaa atttccaagg cccgaaaatt cgccgtggaa gcttttgccg agagcctgct
     3421 ccccgtggcg gacagcctgg aagccggtct gatcatcaag gatgccactg ccgaacaaat
     3481 tcgcgagggc gcacaggcca cattgcgcca gttgcttgcc gcactggagc gcaacaaggt
     3541 ccttgccatc aacccgtcgg caggcaccaa atttgaccca catcaacacc aggccatcag
     3601 cgttgtacct tcggagcagg aagccaacac cgtcgtgagc gttctgcaaa aaggctattc
     3661 gattgccgac cgggtactgc gcccggcact ggtgaccgtc tcggcaccca aataaagtaa
     3721 gaaaggttgc gccactccgg tcaacctggt tttctgcatc cgggcccttg aaatagccct
     3781 ataggcccac aagttatcca catccaagtt ttttcagaat atcaggagta aatcatggga
     3841 agaatcatcg gcattgacct cggcaccacc aacagctgtg tcgccatcat ggagggcaat
     3901 acgcccaagg ttatcgagaa cgcagaaggt gcgcgcacta cgccgtccat cattgcgtac
     3961 caggaagacg gtgaggtgct ggtcggtgcg tctgccaagc gccagtcggt gaccaacccc
     4021 aagaacacgc tgtacgcagt caagcgcctg atcggccgca agttttccga aaaggaagtg
     4081 cagaaggaca tcgacctgat gccctacaag attgccgctg ccgacaacgg cgatgcctgg
     4141 gtggaagtgc gtggcaaccg catggcaccg cagcaggtca gcgccgacat cctgcgcaag
     4201 atgaagaaga ccgccgaaga ctatctcggc gaagaagtga ccgacgccgt gatcacggta
     4261 cctgcctact tcaatgatgc acagcgccag gcaaccaagg atgcaggccg tatcgccggt
     4321 ctggacgtca
//
LOCUS       DAOSJM010000203         4328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_17928_length_4328_cov_3.513457, whole genome shotgun sequence.
ACCESSION   DAOSJM010000203 DAOSJM010000000
VERSION     DAOSJM010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17928_length_4328_cov_3.513457"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PKX82_08090"
     CDS             complement(<1..818)
                     /locus_tag="PKX82_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF52254.1"
                     /translation="MKAAAPLLNVQGLRIAFGGREVVRGISYTVAAGEKLALVGESGS
                     GKTVSALSLLGLVQGASVQGQALFTGAQGQPGAVDLLTLPERALQVIRGQDIAMIFQE
                     PMTALNPLFSIGEQIAEVLQQKMGLAPAESARIAIDLIANTGIEDPERRARAYPHQLS
                     GGQRQRAMIAMALACKPKLLLADEPTTALDVTVRKQILELLAQLQRKHGMAVILITHD
                     LNLVRQFADRVAVMEDGCIVEQGAVAEVFARPQHAYTRRLIESRPTRNVLEPVP"
     gene            complement(815..1843)
                     /locus_tag="PKX82_08095"
     CDS             complement(815..1843)
                     /locus_tag="PKX82_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF52255.1"
                     /translation="MAQLSPGRRAWQRFRRNRTGFVSLIVFCVLVGLSLVAELVSNDR
                     PLVVVYQGQMYMPMFKDYPETTFSGDFATPTDYLDPFIQKKLAEPGNWAVFAPNRYGP
                     KTLNYFAPAPNPAPPGRANWLGTDDRGRDLLAQLIYGFRVSVLFALALTMTGVGLGVI
                     TGAIQGFFGGKTDLAFQRFIEIWGAMPELYLLIIFSAVLAPSIALLLILLSLFGWMGL
                     SDYVRAEFLRNRQMDYVRAARALGVSNWKIITRHLLPNSLTPVVTFLPFRMSGAILAL
                     TSLDFLGLGVPPGTPSLGELLSQGKNNIDAWWISLSTFGVLVMTLLLLTFMGDALRDA
                     LDPRKADA"
     gene            complement(1929..2957)
                     /locus_tag="PKX82_08100"
     CDS             complement(1929..2957)
                     /locus_tag="PKX82_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOF52256.1"
                     /translation="MLLYSLKRLLLMVPTLLGVLFLTFVVIQFVPGGPVEQYLAEAKA
                     GAGSGAEGGGLSYRGAQGVDPKRIEQAKALYGFDKPAPERFWQMLKQFAQFDLGKSFF
                     QNRDVSALIWEKLPVSMSLGLWTFFISYLVAVPLGVAKAVRAGSRFDLVTTLLVLVGY
                     AIPGFVLGVALLVIFGGQLQWFPLRGLTSANWDTLSWGARITDYLWHIALPVTSMVLG
                     SFAVTAMLTKNAFLEEIRKQYVVTARAKGLSERQVLWKHVMRNALIPIITGFPAAFVG
                     AFFTGSLLIETLFSLDGLGLLSYESVIRRDYPVVMGTLYLFTLIGLVTKLLSDLCYVW
                     ADPRIKYD"
     gene            complement(2961..>4328)
                     /locus_tag="PKX82_08105"
     CDS             complement(2961..>4328)
                     /locus_tag="PKX82_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOF52257.1"
                     /translation="PGIRSQLEDVAGVDVVDSTTVRYRFKKPNRELPLTVGGLPVFSH
                     RWGESDAAGGGKPKPFDQVVMDIPIGSGPYRMGPVNFGKDITYVRDASYWAKDLNVRR
                     GSANFDRITVKIYKDNTARLEALKAGEFDLMRFFSAGDWARRVTGRKFDSGELVKAEF
                     KHRLPTGFQSYVLNTRREQLKDVRVRQALGLALDYEWMNRQMFYGAYQRVQGLFGNTD
                     CQANGLPDAAELALMEPFRAQLPASTFGPVATPPRTDGDSSLRDNLRRAQQLLKDAGW
                     EYRDGALRNAKGEPFVLEYLDSNEGGARVVTPWARNLEKIGVQLKFRPVDFALYQQRL
                     QKFEFDITSLAYQGTHNPGQEFADLFGSKAADQEDSGNHSGVKNVAVDALITRITSAN
                     TKAQLLPACRALDRVIGHLHLLVPQWSAGTHRMAYNAWRLAKPDTMPPYAAGEGWAID
                     TWWAK"
BASE COUNT          903 a         1403 c         1261 g          761 t
ORIGIN      
        1 tcgggcaccg gctccaacac gttgcgcgtt gggcggctct cgatgagacg gcgggtgtag
       61 gcatgctgtg gccgggcaaa tacctcggcc acggcaccct gctccacgat gcagccgtct
      121 tccatgaccg ccacgcggtc cgcaaactgg cgcaccagat tcaggtcgtg cgtgatcagg
      181 atcaccgcca tgccgtgctt gcgctgcagc tgcgccagca gttccagaat ctgcttgcgc
      241 accgtcacgt ccagcgccgt ggtcggctca tctgcgagca ggagcttggg tttgcaggcc
      301 agtgccatgg cgatcatggc ccgctgacgc tggccaccag acagctggtg cgggtaggca
      361 cgggcgcggc gctcaggatc ttcgatgccg gtatttgcta tcaaatcaat agctattcgc
      421 gcagattcgg cgggggctag acccattttt tgctgaagaa cctcggcaat ctgctcgcca
      481 atgctgaaca aggggttgag cgccgtcatg ggctcctgga agatcatcgc gatgtcctgc
      541 ccgcgaataa cctgcaaggc acgctccggc agggtcaata gatctacagc gcccggttgg
      601 ccttgcgctc ccgtaaatag ggcctgcccc tgcacgctgg ccccctgcac caagcccagc
      661 aggctgaggg cggagaccgt cttaccagag cccgattccc ccaccagtgc cagcttttca
      721 cctgcggcca cggtatagct gataccccgg actacttcac ggccgccaaa agctatccgc
      781 aagccctgga cattgagcaa aggcgctgcg gccttcatgc gtcggccttt cgggggtcca
      841 gcgcatcccg cagcgcatcg cccatgaagg tcagcagcag cagcgtcatg accagcacac
      901 caaaggtgga cagagagatc caccaggcat cgatattgtt cttgccttgc gacagcaact
      961 cgcccagcga gggcgtgcca ggcggtacgc ccaggccgag aaagtccagc gaggtcagcg
     1021 ccagaatcgc gccgctcatg cgaaacggca gaaaggtgac caccggtgtc aggctgttgg
     1081 gcagcaggtg gcgggtgatg atcttccagt tggagacacc cagtgcccgt gccgcgcgca
     1141 catagtccat ctgccggtta cgcagaaact ccgcccgcac atagtccgac agacccatcc
     1201 agccaaacag gctcagcagt atcaatagca gggcaatgct gggcgccagc accgcactga
     1261 aaataatcaa caggtacagc tcgggcatgg ccccccatat ctcgatgaag cgctggaatg
     1321 ccagatcggt cttgccgcca aaaaagccct gaatggcgcc ggtgatcacc ccgagcccca
     1381 cgccggtcat ggtcagcgcc agcgcaaaca acacgctgac ccgaaagcca taaattagtt
     1441 gggccagcag atcccgcccc cggtcgtcgg tgcccagcca gttggcacgt cccggtggcg
     1501 cgggattggg cgctggagca aagtaattca aggtcttggg accgtagcgg ttgggcgcaa
     1561 acacggccca gttgcccggc tcggccagct tcttctggat gaacgggtcc agatagtcgg
     1621 tgggcgtggc aaagtcaccg ctaaacgtgg tctccgggta atccttgaac atgggcatgt
     1681 acatctgccc ctggtacacc acgaccagtg gccggtcatt cgacaccagt tctgccacca
     1741 ggctcaagcc caccagcaca caaaatacga tcagactgac aaagcccgta cggtttcgcc
     1801 taaagcgctg ccaagccctt ctacccggag agagttgcgc catgtcaggg ccgccgcaga
     1861 gccggcttgg ccgggctgct ggtggcgccc ccccgagggg ggaggcagcc gcaggctgct
     1921 tcggggggtt agtcatattt aatacgcggg tccgcccata catagcaaag gtccgaaagc
     1981 agcttggtca ccaggccaat caaggtaaac aaatacagcg tccccatgac gacgggatag
     2041 tcccgccgga tcacgctctc gtaactcaag agccccagac cgtcaagcga gaacaaggtc
     2101 tcgatcaaca gcgaaccagt gaagaaagca ccgacaaaag cggccggaaa gccggtgatg
     2161 atgggaatca aggcattgcg catcacatgc ttccacagca cctgccgctc ggacagccct
     2221 ttggcgcggg cggtcaccac atactgcttg cggatctctt ccagaaaagc atttttggtc
     2281 agcatggccg tcaccgcaaa actgcccagc accatggacg tgacgggtag cgcgatatgc
     2341 cacaggtagt cggtgatgcg cgcgccccag ctcaaggtgt cccagttggc agaagtgagc
     2401 ccgcgcagtg gaaaccactg caactgaccg ccaaaaatca ccagcagcgc cacgcccagc
     2461 acaaagcccg gaatcgcata gcccaccagt accagcagcg ttgtcaccag gtcaaatcgc
     2521 gaaccggcac gcaccgcttt ggccacaccc agcggtacgg ccaccaggta gctgatgaag
     2581 aaggtccaca gcccgaggct catggacacc ggcaacttct cccagatcag tgcggagaca
     2641 tcgcggttct gaaaaaagct tttgcccagg tcaaactgcg caaactgctt gagcatctgc
     2701 cagaagcgct ccggagccgg cttgtcgaaa ccgtacagtg ctttggcttg ttcaatgcgc
     2761 ttggggtcta caccctgcgc gccccggtaa ctcaggccac cgccttcggc gccactgccc
     2821 gcgccggcct tggcctccgc caggtactgc tcgaccggac ctcccggcac aaactggatg
     2881 acgacaaacg tcaggaacaa cacccccaac agggtgggca ccatcagcag caagcgtttc
     2941 agggaataca gcagcatggc ctattttgcc caccaggtgt caatcgccca accttcgccg
     3001 gcggcatacg gcggcatggt gtcgggtttt gccaaacgcc aagcgttgta ggccatgcgg
     3061 tgcgtgcccg cagaccattg gggcaccagc aggtgcagat ggccaatgac gcgatcaagc
     3121 gcgcggcagg ccgggagcag ttgcgccttg gtgttggcgc tggtaatgcg cgtgatcaag
     3181 gcatccaccg ccacattctt gacaccgctg tggttgccgg aatcctcctg gtccgccgcc
     3241 ttgctgccaa acagatcggc aaattcctgc cccgggttgt gggtgccctg gtaggccaaa
     3301 gaggtgatgt cgaattcaaa tttctgcagg cgctgctggt acagcgcaaa gtccaccgga
     3361 cggaacttga gttgtacgcc gatcttttcc agattgcggg cccagggcgt caccaccctt
     3421 gcgccaccct cgttgctgtc caggtactcc agcacaaaag gctcaccttt ggcgttgcgc
     3481 aaagcaccgt cacggtactc ccagccggcg tctttgagca gttgctgcgc gcggcgcagg
     3541 ttgtcgcgca ggctgctgtc gccatcggtc cgcggcggcg tggccaccgg cccaaacgtg
     3601 gacgcaggca actgcgccct gaaaggctcc atcagcgcca gctcggcagc atccggcagg
     3661 ccattggcct ggcaatcggt gttgccaaac aggccttgca cccgctggta ggctccgtaa
     3721 aacatctgac ggttcatcca ctcgtaatcc agcgcaagcc ccaatgcctg gcgcacccgc
     3781 acatccttga gctgctcgcg ccgggtgttg agcacatagc tttgaaagcc cgtgggcagg
     3841 cggtgtttga actcagcctt gaccaattcg cccgagtcaa acttgcgccc ggtcacgcgt
     3901 cgcgcccagt cacctgcgga aaagaaacgc atcagatcaa attcaccggc cttcaaggct
     3961 tccagccggg cggtgttgtc cttgtagatc ttgacggtga tgcggtcgaa gttggcgctg
     4021 ccgcggcgta cattgaggtc tttggcccag tagctggcat cgcgcacata ggtgatgtcc
     4081 ttgccaaagt tgaccggacc catcctgtac ggcccgctgc caatcggaat atccatcacc
     4141 acctggtcaa acggcttggg cttgccgccc cccgctgcgt cgctttcgcc ccagcggtgg
     4201 ctgaacaccg gcagcccccc cactgtcagc ggcaactccc ggttgggctt cttgaaccgg
     4261 tagcggacgg tggtggaatc gaccacgtcc acaccggcca catcttcgag ctggctgcga
     4321 atacccgg
//
LOCUS       DAOSJM010000204         4280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18202_length_4280_cov_4.077870, whole genome shotgun sequence.
ACCESSION   DAOSJM010000204 DAOSJM010000000
VERSION     DAOSJM010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18202_length_4280_cov_4.077870"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKX82_08110"
     CDS             complement(<1..381)
                     /locus_tag="PKX82_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOF52258.1"
                     /translation="MSQNQFDKKSQAWSALFSEPMSELVKRYTSSVFFDKRMWQADIT
                     GSLAHADMLAAQGIINAADHASIQSGMATIWSEIESGAFEWKLDLEDVHLNIEARLTQ
                     LVGDAGKRLHTGRSRNDQVATDVRL"
     gene            421..1458
                     /locus_tag="PKX82_08115"
     CDS             421..1458
                     /locus_tag="PKX82_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOF52259.1"
                     /translation="MKDTQILSAFHELPSGTEELPPAPALVFDACHMGVVLRAVLFVE
                     LSVAAVVMFFTQSLLDWVTGLSLVTGAALPATLAWLLTACAAKKLLARVAAGVQYAVA
                     VTLGALAGMYGCGLLWFSGLVQQAPWVASAFSGALISAVLVAALVMRAKGRGPANTAA
                     RLEELQSRIRPHFLFNTLNSAIALVRAEPAKAEALLEDLSDLFRHALIDQGESVTLAD
                     EVALARRYLDIEQVRFGDRLRVQWVLDPNAQGALLPPLLLQPLVENAVRHGVEPSPGG
                     ADVKISTERRGSVVVVKVTNTVPAGPGVAGNGVALKNVRERLRLLHDVQGQFRSGLKN
                     GVYQVRMEVPA"
     gene            1455..2213
                     /locus_tag="PKX82_08120"
     CDS             1455..2213
                     /locus_tag="PKX82_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOF52260.1"
                     /translation="MTVAMTPLRALVVDDETLARSRMRTLLGDCRNPEVQVVAEAANA
                     VQAMDQLQRQVFDLVLLDIHMPGTDGLALAQSLRALPQPPAIIFVTAHAEHAVQAFEL
                     EAVDYLTKPVRLERLQASLQKVERFVQSRLGLEADSTTNEFLLIQDRNRTERVPLHQV
                     VYFKAELKYITVRTLERSFILDGSLGELEDKYGDNFVRIHRNALVARRAIRALEKHYD
                     PVEGDGWAARLHGVDELLAVSRRQLASVRDILAH"
     gene            complement(2226..>4280)
                     /gene="ppc"
                     /locus_tag="PKX82_08125"
     CDS             complement(2226..>4280)
                     /gene="ppc"
                     /locus_tag="PKX82_08125"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOF52261.1"
                     /translation="GNHPVHSFLRMGQWIGGDRDGNPNVSAQTLEYALRRQAEVALRH
                     YLTEVHYLGSEPSISAMLSDVSPEMQALADHSPDRNEHRKDEPYRKALTGVYARLAAT
                     LKDLTDTEAARHAVAPQNAYLKAEDFIADLRTIEASLQANHGSALVSQRLHPLIRAVE
                     VFGFHLATVDLRQSSDKHEEVVAELLATARIAPNYSSLDEAAKRSLLTGLLNDARALR
                     VHGVSYSAHTEGELAIFAMAKVMLERFGKQSIRHYIISHTETVSDLLEVLLLQKEVGL
                     MRGTLDSDATCDLIVVPLFETIEDLRNAAPIMREFYALPGAAKLVQRSGAEQDIMLGY
                     SDSNKDGGIFTSNWELYRAEIALVDLFDQLANSHNIQLRMFHGRGGTVGRGGGPSYQA
                     ILAQPPGTVRGQIRLTEQGEVIGSKYANPEIGRRNLETLVAATLEATLLQPTKSATKA
                     FLDAAAELSQNSMAAYRALVYDTPGFTEYFFSSTPIREIAELNIGSRPASRKASQKIE
                     DLRAIPWGFSWGQCRLTLPGWFGFGAAVNTFVHSGTPQEAKERTALLQKMYKQWPFFR
                     TLLSNMDMVMAKSDLALASLYAELVSDARLRKKIFAAIEAEWHATAQALVTITGEKQR
                     LANNAALQRSIRHRFPYIDPLHHLQVELVRRYREGKADERVQRGIHISINGIAAGLRN
                     TG"
BASE COUNT          701 a         1232 c         1489 g          858 t
ORIGIN      
        1 caggcgcacg tcggtggcca cctggtcatt gcggctgcgg ccggtgtgca ggcgtttgcc
       61 cgcgtccccc accaattggg tcaggcgcgc ttcgatgttg aggtgcacgt cttcgaggtc
      121 gagcttccat tcaaacgcac cggactcgat ctcggaccag atggtagcca tgccactttg
      181 gatggatgcg tggtctgcgg cgttgatgat gccttgcgct gccagcatgt cggcgtgggc
      241 gagggaaccg gtgatgtcgg cctgccacat gcgcttgtca aagaacacgc tggaggtgta
      301 gcgcttgacc agttcgctca tgggctcgga gaacagcgcg gaccacgcct gggatttttt
      361 gtcgaattgg ttttgggaca tgggctgcag gggagtgaat ggagtcaaaa tccgggaaac
      421 atgaaagaca cccaaatatt atcggcgttc cacgaactgc cctcgggaac tgaggaactg
      481 ccgcctgcgc ccgccctggt gtttgatgcc tgccatatgg gcgtggtgct gcgcgcggtg
      541 ctgtttgtgg agctgtcggt agccgctgtg gtgatgtttt tcacccagag cctgctggac
      601 tgggtgacgg gtctgtcgct ggtcacgggg gcggccctgc cggccacgct ggcctggctg
      661 ctgacggctt gtgctgccaa gaagctgctg gcccgtgtcg cggcaggggt gcaatacgcg
      721 gtggccgtga cgctgggtgc gttggcgggc atgtacgggt gtggcctgtt gtggttctcg
      781 ggcttggtgc agcaggcacc ctgggtggcc agtgcattca gtggtgcatt gatttcggcg
      841 gtgctggtgg cggccctggt catgcgtgcc aaggggcggg gacccgccaa tacggcggcg
      901 cggctggaag agttgcagtc gcgcatacgc ccgcactttc tgttcaacac actcaacagc
      961 gccatcgctc tggtgcgcgc cgaacctgcc aaggccgaag ccttgctgga agacctgagc
     1021 gatctgtttc gccatgccct gatcgaccag ggggagtcgg tcacgctggc cgacgaagtg
     1081 gctctggcca ggcggtacct ggatattgag caggtgcgct ttggcgaccg gttgcgggtg
     1141 cagtgggtgc tggatcccaa cgcacaaggc gccttgttgc cgccgctgct gctgcagccg
     1201 ctggtggaaa acgccgtgcg ccatggggtc gagcccagcc cgggcggcgc agacgtgaaa
     1261 atttctaccg aacgccgcgg atcggtggtg gtggtcaagg tgaccaatac cgtgcctgcc
     1321 gggcctgggg tggcgggcaa cggcgtggcc ttgaagaacg tgcgcgagcg cctgcggctg
     1381 ctgcacgacg tacagggaca gttccgcagc gggctgaaga atggtgtgta ccaggtgcgt
     1441 atggaggttc ctgcatgacg gttgcgatga cgcccttgcg ggcactggtg gtggatgatg
     1501 aaaccctggc ccgttcccgc atgcgcaccc tgctggggga ttgccgcaat cctgaggtgc
     1561 aggtcgtagc cgaggcggcc aacgccgtgc aggccatgga ccagctgcag cggcaggtgt
     1621 tcgacctggt gctgctcgat atccacatgc cggggaccga tgggctcgcg ctggcgcaat
     1681 cgctgcgcgc cttgccacag ccgcccgcaa tcatctttgt gaccgcccac gccgaacatg
     1741 cggtgcaggc gtttgagctg gaagcggtgg actacctgac caaaccggtg cggctggagc
     1801 ggttgcaggc ttcgctacaa aaagtagagc gcttcgtgca atctcgactg gggctggagg
     1861 ccgattcgac cacaaatgaa tttctgttga tccaggaccg caaccgtacc gaacgcgtgc
     1921 cgctgcacca ggtggtgtat ttcaaggccg agctcaagta catcacggtg cgcacactgg
     1981 aacgcagttt tatcctggac ggctcgctgg gcgagctgga agacaagtac ggcgacaact
     2041 ttgtgcggat ccaccgcaac gcgctcgtgg cgcgccgtgc gatccgggct ctggaaaaac
     2101 actacgaccc ggtggaaggc gacggctggg ccgcacgcct gcacggtgtg gatgagttgc
     2161 tggcagtgtc acgccgccag ctcgccagcg tccgggacat tctggcccac tgagcggtaa
     2221 ggctgttagc cagtattgcg cagacccgcg gcgatgccgt tgatcgagat gtggataccg
     2281 cgctgcaccc gttcatcggc tttaccttcg cggtagcgtc gcaccagttc cacctgcagg
     2341 tggtgcagcg gatcgatgta gggaaagcgg tggcggatgg agcgctgcag tgcggcgtta
     2401 ttggccaggc gctgcttctc tccggtgatg gtgaccagcg cttgtgcggt ggcatgccac
     2461 tcggcttcaa tcgccgcaaa aatcttcttg cgcaggcgcg catcgctcac cagttcggca
     2521 tagagcgaag ccagtgccag atcgcttttg gccatcacca tgtccatgtt ggagagcagg
     2581 gtgcggaaga acggccactg cttgtacatc ttttgcagca aggcggtgcg ctctttggct
     2641 tcctgtggcg tgccactgtg cacaaaggtg ttgaccgccg caccaaagcc aaaccagccc
     2701 ggcagggtca ggcggcactg gccccagctg aagccccagg gaatcgcccg caggtcttca
     2761 atcttttgcg aagccttgcg cgatgcgggg cgcgagccga tgttgagttc ggcaatctcg
     2821 cggatcgggg tggaactgaa gaagtattcg gtaaagcccg gggtgtcgta gaccagcgcg
     2881 cggtaggccg ccatgctgtt ctgtgacaac tcggccgcag catccagaaa cgccttggtg
     2941 gcggacttgg tgggttgcag cagggtggct tcgagggtgg ctgccaccag ggtttccagg
     3001 ttgcgccggc cgatttctgg gttggcgtat ttggagccaa tcacctcgcc ctgctcggtc
     3061 agccggatct gcccgcgcac ggtgcctggg ggctgggcca gaatggcctg gtagctcggg
     3121 ccgccgccgc ggcccacggt gccgccacgg ccgtggaaca tgcgcagctg gatgttgtgg
     3181 gaattggcca gctggtcaaa caggtccacc agcgcaattt cggcgcggta cagctcccag
     3241 ttgctggtga agatgccacc gtccttgttg ctgtcggagt agcccagcat gatgtcctgt
     3301 tcggcgccgc tgcgctgcac cagcttggcc gcgccgggca gggcatagaa ctcgcgcatg
     3361 atgggggccg cgttgcgcag gtcttcaatg gtctcgaaca gcggcaccac gatgagatcg
     3421 caggtcgcat cgctgtcgag ggtgccgcgc atcagcccca cttctttctg cagcaacagc
     3481 acttccagca ggtcactgac cgtttctgtg tggctgatga tgtagtggcg gatggactgc
     3541 ttgccaaaac gctccagcat gactttggcc atggcaaata tggccagttc gccttctgtg
     3601 tgggcgctgt agctgacacc gtgcacgcgg agtgcgcggg cgtcgttgag caggccggtc
     3661 agcagactgc gcttggcagc ttcgtcgagg ctgctgtagt tcggggcaat gcgggcggtg
     3721 gccagcagtt cggccaccac ttcttcgtgt ttgtccgagc tctggcgcaa gtccacggtg
     3781 gccaggtgaa aaccaaacac ctccacggcg cggatcagcg ggtgcagtcg ctggctgacc
     3841 agcgcagaac cgtggttggc ctggagcgag gcttcaatcg tgcgcaggtc ggcaatgaaa
     3901 tcctccgcct tcaggtaggc gttttgcggc gcgacggcat ggcgtgcagc ttccgtgtcc
     3961 gtcaggtcct tgagcgtggc ggccagtcgg gcgtagacgc ccgtcagcgc cttgcggtag
     4021 ggctcgtcct tgcggtgttc gttgcggtca ggggagtggt ctgccagcgc ctgcatctcg
     4081 gggctgacgt cggacaacat cgcggagatg gacggttcgc tgcccaggta gtgcacttcg
     4141 gtcaggtagt ggcgcagtgc cacttcggcc tggcggcgca gggcgtattc cagggtctgg
     4201 gcactgacgt tggggttgcc atcgcggtcg ccgccgatcc actggcccat gcgcagaaag
     4261 ctgtgcaccg ggtggttgcc
//
LOCUS       DAOSJM010000205         4272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18234_length_4272_cov_4.327247, whole genome shotgun sequence.
ACCESSION   DAOSJM010000205 DAOSJM010000000
VERSION     DAOSJM010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18234_length_4272_cov_4.327247"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="PKX82_08130"
     CDS             <1..355
                     /locus_tag="PKX82_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229102.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOF52262.1"
                     /translation="GYMQITDRSKDVIKSGGEWISSIDVENIAMAHPAVAMAACIGMK
                     HPKWDERPVIAVVKKPGIEVTREELIAFYEGKTAKWQIPDDVVFVDAIPLGATGKMQK
                     IKLREMLQDYKLPGI"
     gene            462..1697
                     /locus_tag="PKX82_08135"
     CDS             462..1697
                     /locus_tag="PKX82_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOF52263.1"
                     /translation="MKFAVQLVAAAAVLACAQGAFAQQGQTVKMVRIDAQTGLLGPVG
                     VNQLKSYQFFAEKFSGAGNPAGVKFEVTGIDNKLSPTESLNALKAAIDGGARYILQGN
                     GSSVALALSDAVTKHNERNPGKEVLYLNDSAVDPDLTNSKCSYWHFRFDADTSMKMEA
                     MSSFMETQKDIKKVYILGQNYSHGVQVAKFAKETLGRKRPDIQIVGEDLHPLAQVRDF
                     APYIAKIKASGADTVITGNWGSDLSLLVKAANEGGYTGKFFTYYTGVTGTPSALGTNG
                     AGRVYQIAYNHYNMGGQMDKWMTEFKAKYNDDFYTGSIIRIYQTLGAAMAKAKSTDPV
                     KVAAALEGIKVSSFNGEVEMRKTDHQLQQPLYMTVWQKADKKYPYSPENTGMTLAPLK
                     EYPNYVSSTPTSCQMKRPS"
     gene            1780..2814
                     /locus_tag="PKX82_08140"
     CDS             1780..2814
                     /locus_tag="PKX82_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF52264.1"
                     /translation="MEFFIISMLNGLSYGLLLFMLSSGLTLIFSMMGVLNFAHASFYM
                     VGAYVGYSVSQIVGFWPALVIAPLVVGALGALFERIALRKVHKFGHVPELLVTFGLSY
                     VLLELVQLIWGRLALEFRPPEILQGPAFTLINHSINGLSVLWGVAPAEMCSAADAAVR
                     VVCSPFPATRGFMMLVALVMLGSVWLLLTRTRIGLVIQAALTHPHMVESLGHNVPMVF
                     MMVFGAGAALAGLAGVIGGSTFLTEPAMAATVGSIIFVVVVVGGMGSLSGAFLASILI
                     GVIQTFAIAFDYSVMSLAQQMGVPLSAGASSNSLVKLTLSQIAPILPYLFLVLILIFR
                     PKGLLGTREG"
     gene            2818..4107
                     /locus_tag="PKX82_08145"
     CDS             2818..4107
                     /locus_tag="PKX82_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF52265.1"
                     /translation="MKNFYEFKPMNVGRLVIWTLFAVPLLLAPLVFKSGLSITVLSQM
                     GIAIIACLSYNMLLGQGGMLSFGHAVYTGLGSYLAIHALNAIADGSLPIPVSTVPLVG
                     GLAGMGFAVLFGYVTTRKSGTTFAMITMGLAELVFAMSLMIPEFFGGEGGVSGNRVTG
                     QPFMGISFGPPLQVYYLIAVYTFVCVALMFAFTGTPLGRMLNAVRDNPERVEFIGYNP
                     QRIRYTAFIIAGFFAGISGGLAALNFEIVTAEVVGAARSGAYLLFTFLGGATFFFGPI
                     IGAILMVLAFVLLSEFTKAWLLYLGLVFLFMVMYAPGGIASLIMMNLRVAKFGHLKSL
                     WVSYLGLAGTACVALTGAAVMVEMIYHRQMDSAMGSELHFMGLALDAAGASSWFGAFF
                     VLATGLGLFELCRRQFVEQWGHIQEDIEKQIQRQEAL"
     gene            4104..>4272
                     /locus_tag="PKX82_08150"
     CDS             4104..>4272
                     /locus_tag="PKX82_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52266.1"
                     /translation="MSNTHATGTPHALELRNLRKSFGKSEIIRGVNLAVQPGERVAII
                     GPNGAGKSTLFN"
BASE COUNT          836 a         1197 c         1248 g          991 t
ORIGIN      
        1 tggctacatg cagatcaccg accgcagcaa ggatgtgatc aagtcgggcg gcgaatggat
       61 cagttccatt gatgtggaaa acattgcaat ggcacacccg gccgtcgcca tggccgcttg
      121 catcggcatg aagcatccca aatgggatga gcgtcctgtg atcgcggtgg tcaaaaagcc
      181 ggggatcgag gtgacgcgcg aagaactgat tgctttctat gaaggcaaaa ccgccaagtg
      241 gcaaattccg gatgacgtgg tttttgtgga tgccattcct ctgggtgcca ccggaaaaat
      301 gcaaaaaatc aagctgcgcg aaatgctgca ggactacaag ctgccgggaa tttgaacaag
      361 cgtgcagtca cattagtgat agctcttggg ggaaaccctt gcgctgcagc gcacaaatac
      421 ttgtacgcta cgtcagcttc gattttttta ggagacaaca gatgaaattt gcagtccaac
      481 tcgttgcggc agccgccgtt cttgcatgtg cccagggtgc attcgcccaa cagggtcaaa
      541 ccgtcaaaat ggtccgcatt gatgcgcaga cgggtcttct tggaccagta ggggtcaacc
      601 agctcaagag ctaccagttt tttgctgaaa aattcagcgg tgcaggcaac cccgcgggcg
      661 tgaagttcga agtgaccggc attgacaaca agctgagccc cacagaaagc ctgaacgccc
      721 tgaaggcggc catcgatggc ggcgcacgct acatcctgca aggtaacggc tcttccgttg
      781 cactggcact gtccgatgct gttaccaaac acaatgagcg gaaccccggc aaggaagtcc
      841 tgtacctgaa tgattcggct gtggatcctg atttgaccaa cagcaaatgc agctactggc
      901 acttccgttt cgacgcagac acctccatga aaatggaagc catgtcgagc ttcatggaaa
      961 cccagaagga catcaaaaag gtttacatcc tgggtcagaa ctattcccac ggtgtacagg
     1021 ttgccaagtt cgccaaggaa actctgggcc gcaagcgtcc tgacatccag atcgtcggcg
     1081 aagacctgca cccactggcg caagtgcgtg acttcgctcc gtatatcgcc aagatcaagg
     1141 cgtccggtgc tgacaccgtg attaccggca actggggctc cgacctgtct ctgctggtca
     1201 aggccgccaa tgaaggcggc tacacaggca agttcttcac gtactacacc ggtgtgacgg
     1261 gtactcccag tgctctgggc accaacggcg ctggccgcgt gtaccagatt gcctacaacc
     1321 actacaacat gggtggtcag atggacaagt ggatgactga gttcaaggcc aagtacaacg
     1381 acgacttcta caccggctcc atcatccgca tttaccagac cctgggtgct gccatggcca
     1441 aagccaagtc gaccgatcca gtcaaggttg ccgcggctct ggaaggtatc aaggtcagca
     1501 gcttcaatgg tgaagtggaa atgcgcaaga cggatcacca gctgcagcaa cccctgtaca
     1561 tgaccgtgtg gcaaaaggct gacaagaaat acccctacag ccccgagaac accggcatga
     1621 cgctggctcc cctgaaggag taccccaact atgtttccag cacgcccacc agctgccaga
     1681 tgaagcgccc atcctgattc agggcgcggt ttccaggagc ccgaaagcgc acagcttcgg
     1741 gctcctttta tttgtctgtg ttaggaaggt atttcggcaa tggaattctt tatcatctcg
     1801 atgctcaatg gcctgagtta cgggcttctg ctgttcatgc tgagctcggg tttgacgctg
     1861 attttcagca tgatgggcgt actgaacttc gcccacgctt cgttttacat ggtcggtgcc
     1921 tacgttggat actcggtatc ccagatcgtg ggcttctggc ccgccctggt cattgcaccg
     1981 ctggtggtgg gtgctttggg tgccttgttt gaacgcatcg ccttgcgcaa ggtccacaag
     2041 ttcggacacg ttcccgagtt gctggtgacg ttcgggcttt cctatgtgct gctggagctg
     2101 gtccagctga tttggggccg tcttgcgctg gaattccgtc caccggaaat tctgcagggt
     2161 cccgccttta ccctgatcaa ccattccatt aatggactca gtgtcctgtg gggcgtagca
     2221 cccgcagaaa tgtgcagcgc tgccgatgct gcggtacgcg tggtgtgctc cccgtttccc
     2281 gcgacccgtg gtttcatgat gctggtggca ttggtgatgc tggggtcggt ctggctgttg
     2341 ctgacgcgca cccgcatcgg tctggtcatt caggcggctc tgacgcatcc gcacatggtg
     2401 gagtcgctcg ggcacaacgt ccccatggtc ttcatgatgg tgttcggtgc aggcgctgcg
     2461 ctcgcaggcc ttgctggcgt cattggcgga agcacattcc tgaccgagcc cgcgatggct
     2521 gcaacggtcg gctccatcat cttcgtggtg gtggtggtgg gaggcatggg ttcgctgtcc
     2581 ggcgcattcc tggcatcgat cctgatcggc gtgatccaga catttgcgat tgcgtttgac
     2641 tattccgtga tgtcgctggc gcagcagatg ggcgtgccac tgagcgcggg cgccagcagc
     2701 aactcgttgg tgaagctcac gctgtcgcag atcgccccaa tcctgcccta ccttttcctt
     2761 gttcttattc tgatcttccg gcccaagggc ctgcttggta cccgggaagg ttaatccatg
     2821 aaaaatttct acgaattcaa gcccatgaat gtggggcggc tggtgatctg gacgctgttt
     2881 gcagtgccgc tcctgctggc tccgctggtt ttcaagagtg ggctctcgat tacggtcctg
     2941 tcgcagatgg gcattgccat cattgcctgt ctgtcctaca acatgctgtt ggggcagggc
     3001 ggcatgttga gtttcggtca cgcggtgtac acggggctgg ggtcgtacct tgccatccat
     3061 gcgctcaatg ccattgcaga cggttcgctg cccattccgg tgtccaccgt accgctggta
     3121 ggtggtcttg ccggcatggg ttttgccgtc ctgttcggct acgtgaccac acgcaaatcc
     3181 ggcaccacct ttgccatgat caccatgggt ctggccgagc tggtatttgc catgtccctg
     3241 atgattcccg agtttttcgg gggtgagggc ggcgtttcag gcaaccgggt gacagggcag
     3301 cccttcatgg gcatctcctt cgggccaccc ctgcaggtgt actacctgat tgcggtgtac
     3361 acctttgtct gcgtggccct gatgttcgca ttcaccggta ccccgctggg gcgcatgctc
     3421 aacgcggtgc gggacaaccc ggagcgggtg gagttcatcg gctacaaccc gcagcgcatc
     3481 cgttacaccg cattcatcat cgcgggcttc ttcgctggta tttccggtgg tctggcagcg
     3541 ctgaactttg aaattgtgac tgccgaagtg gtgggcgccg cccgttcagg tgcgtacctg
     3601 ctgtttacct tcctgggtgg cgctacgttc ttcttcgggc ccatcatcgg cgccattctg
     3661 atggtgctgg cgtttgtgct gctcagcgag ttcaccaaag cctggctgct gtatctgggt
     3721 ctggtgttcc ttttcatggt catgtacgcc cccggtggta ttgccagtct gatcatgatg
     3781 aacctgcggg tcgccaaatt cggccacctc aagtctttgt gggtcagcta cctcggcctg
     3841 gctggcacgg cctgtgtggc gctgaccggc gcagctgtca tggtggagat gatttaccac
     3901 cgccagatgg attccgcgat ggggtccgag ctgcacttca tgggcttagc tctggatgcg
     3961 gccggtgcaa gcagctggtt tggagcattc ttcgtactgg cgaccggttt gggattattt
     4021 gaactgtgca ggcggcaatt cgtggagcaa tggggccata tccaggaaga catcgagaaa
     4081 cagatccagc gtcaggaggc cctatgagca acacacatgc aaccggcacg ccccatgctc
     4141 tggaactgcg caacctgcgc aagagcttcg gcaaatcgga gatcatccgg ggtgtcaatc
     4201 tggctgtgca acccggtgaa cgggtggcca tcatcgggcc caatggagcc ggtaaatcca
     4261 cgctgttcaa cc
//
LOCUS       DAOSJM010000206         4261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18310_length_4261_cov_3.457680, whole genome shotgun sequence.
ACCESSION   DAOSJM010000206 DAOSJM010000000
VERSION     DAOSJM010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18310_length_4261_cov_3.457680"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PKX82_08155"
     CDS             complement(<1..1079)
                     /locus_tag="PKX82_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOF52267.1"
                     /translation="MTSKKNIEILRVTHLRGPNIWTYRPVIEAWVNIGELEDHPSNTL
                     PGFYERLVALLPGLVEHHCSPGVRGGFLQRVREGTWTAHILEHVTLELQNLAGMRTGF
                     GKARQTSTRGVYKIAFRTRQEDVGRAALNAARDLVLAAIHDTPFDIAAVVADLRDKVD
                     SLCLGPSTAHIVDAATDRRIPHIRLTEGNLVQLGYGIAQRRIWTAETDQTSAIAEEIA
                     SDKDLTKSLLKACGVPVPEGELVRSAEKAWEAAQDIGVPVVVKPYDGNHGRGVSLDLR
                     TQSDVEAAYQLAHRKGGGSAVIVEKYIPGNEHRLLVVGRRVVAAASGESLSVIGDGLS
                     TIDQLADAQINTDPRRGTTEDAPLNV"
     gene            1239..3503
                     /locus_tag="PKX82_08160"
     CDS             1239..3503
                     /locus_tag="PKX82_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF52268.1"
                     /translation="MTTEVTAVRPPAGDLSAIARDQVQKQLHPQENVLAALEVDLDSQ
                     LRFVRGLVVVTSQRVLSRVNGDAQWEQWPIAEGMQLQLHDHGGVGHLNLQDRNGLLKT
                     WRFTLGQNLQAIRVVDQFQAQLESTVSGRPMVQALRNVCPTCKAPLEPDQEECPICTK
                     VVHTPPSTWTLFRLWRFAKPYRGQLSLGFVLLLLSTAAAQVPPYLTVPLVDEVLIPFQ
                     NGKHIETSTIAWYLSGLLGAGLLAWVLSWGKTYILALASERIAADLRTTTYEHLLRLS
                     LEYFGGKRTGDLLSRVGSESDRIAVYLSLHLTDFASDVVMIAMTAVILFSMDPYLALI
                     TLVPLPFIGWMIHFVRFRLRTGFEKIDRVWGEVTNVLADTIPGIRVVKAFAQEHREAK
                     RFHDANKHNLVVNDRINKIWSLFSPSVSLLTEIGLLVVWGFGIWQVAQGEITVGMLLA
                     AIAYIGRFYGRLDSMSRIVSMTQKSASAAKRIFDILDHVSSVPEPVNPVPMGKVEGRI
                     EVREAGFRYGNREVNKGINLSIAPGEMIGLVGHSGSGKSALVNLICRFYDVSEGAILV
                     DGVDIRSYAIADFRRNIGLVLQEPFLFFGTIAENIAYGKPDATRGEIIAAARAAHAHE
                     FILRLPQGYDSMVGERGQGLSGGERQRISIARALLIDPRILILDEATSSVDSETEKEI
                     QKALENLVQGRTTIAIAHRLSTLHRADRLVVLDRGRVVEEGTHDALMAQEGAYYALYQ
                     AQARNMDVDMDDAR"
     gene            3500..3991
                     /locus_tag="PKX82_08165"
     CDS             3500..3991
                     /locus_tag="PKX82_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HOF52269.1"
                     /translation="MSTLKTVTTTRFALERNAFGKLILTDAAGERFEGVVPVRAFPIQ
                     SPEDGIALVSTDGHEVGWVDGLEELPGPVQDLIRQELTSREFMPVLERIVDVSSFSTP
                     CTWTVVTDRGETDFVLRGDEDIRRIGKAGSLMIADTHGIQYFVRDQYALDAHSKKILD
                     RFM"
     gene            complement(4022..>4261)
                     /locus_tag="PKX82_08170"
     CDS             complement(4022..>4261)
                     /locus_tag="PKX82_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52270.1"
                     /translation="EPVADGVDHDHVGPLERGPDGQGTRTLNYLFVEAAFSAPQSPQQ
                     EFFVRLHHRCAVYDLLNNYGANGEDFLTVGYRYRF"
BASE COUNT          768 a         1287 c         1352 g          854 t
ORIGIN      
        1 acattcagcg gggcgtcttc ggtggttcca cggcggggat cggtgttgat ctgggcatcg
       61 gccagctggt caatggtgga gaggccatcg ccaatgaccg agagggattc gccgctggcg
      121 gcagcgacca cgcgtcggcc cactaccagc aggcggtgct cgttaccggg gatgtatttc
      181 tcgacaatga cggcacttcc gccacccttg cggtgggcca gctggtaggc agcctcgaca
      241 tcactctggg tgcgcaggtc cagtgacacg ccccgaccat ggttaccgtc gtaaggtttg
      301 accacaacag gcacaccaat gtcctgcgcg gcttcccagg ctttttctgc gctgcgaacc
      361 agttcgccct ccggaaccgg aacgccgcag gccttgagca gcgatttggt caggtccttg
      421 tcgctggcaa tttcctcggc aatggcgctg gtctggtcgg tttccgccgt ccagatgcgt
      481 cgctgggcaa tgccgtaccc cagttgcacc agattgcctt cggtcagccg gatatgggga
      541 atgcgacggt cggtggcggc gtccacgata tgggcggtac tgggccccag gcacagggag
      601 tccaccttgt cgcgcaggtc tgccaccaca gcagcaatgt caaacggcgt gtcgtggatc
      661 gcggccagta ccaggtcgcg agcggcattc agggctgcac ggcctacatc ttcctgccgg
      721 gtgcgaaatg caatcttgta aacaccgcgc gtggaggtct ggcgggcctt gccgaaacct
      781 gtgcgcatgc cggcgaggtt ttgcagctcc agcgtgacat gttccaggat gtgggctgtc
      841 caggtgcctt cgcgcaccct ttgcaggaag ccgccgcgca cgcccgggct gcaatggtgc
      901 tcgaccaggc ccggaagcag ggctaccagc cgctcataga agccgggcag cgtgttggac
      961 ggatggtctt cgagttcgcc aatgttgacc caagcctcga tcacggggcg gtaagtccag
     1021 atattggggc cacgcaggtg tgttacccgg agaatttcaa tatttttctt gctcgtcatg
     1081 gctctttgtg gattgggcag tccaccaacc tggcgctgtc cgcgttatgt attctctctc
     1141 acctggcctc gaattgactg ccgaccaggc atcctggggg tagtatcgtc aagttatcac
     1201 gattgccgat cgcacgtaaa tcctaactgt tgttgttaat gaccaccgaa gtaaccgccg
     1261 tccgcccccc cgcaggagac ctgtccgcca ttgcgcgtga ccaggtgcag aaacagctcc
     1321 acccccagga aaacgttttg gccgcgctgg aggttgacct tgacagccaa ttgcgctttg
     1381 tccgggggct ggtggtggtg accagccagc gcgtgctcag ccgtgtcaac ggcgatgccc
     1441 agtgggagca gtggcccatt gccgagggca tgcaactgca gctgcacgac catggcggcg
     1501 ttggccacct gaatctgcag gaccgcaacg gtctgctcaa gacctggcgg ttcacgctgg
     1561 ggcaaaacct ccaggcgatc cgtgtggtgg accagttcca ggcgcaactg gaaagcacgg
     1621 tctcggggcg gcccatggtg caggcgctca gaaacgtctg tcccacctgc aaggccccgc
     1681 tggagccgga ccaggaggag tgcccgatct gtaccaaggt ggtccatacc ccgccctcta
     1741 cctggacgct gtttcgcctg tggcgctttg ccaaacccta ccgtggccag ttgtcgctgg
     1801 gctttgtact gcttctgctg agcacggccg cggcgcaggt gccgccgtac ctgacggtgc
     1861 cgctggtgga cgaggtgctg attccattcc agaacggcaa acacattgaa accagcacca
     1921 tcgcctggta cctgtccggg ctgttgggtg ccggcttgct ggcatgggtg ctgagctggg
     1981 gcaagaccta cattctggcg ctggcgtccg agcgcattgc tgccgatctg cgcaccacga
     2041 cttacgaaca cctgttgcgg ctctcgctgg agtattttgg cggcaagcgc accggtgatc
     2101 tgctgtcccg cgtcggcagc gagagtgacc gcattgcggt gtacctgtcg ctgcacctga
     2161 cggactttgc cagcgatgtg gtcatgatcg ccatgacggc ggtcatcctg ttttccatgg
     2221 acccgtatct ggcgctcatc acattggtgc cccttccatt catcggctgg atgatccatt
     2281 ttgtgcgctt ccgcctgcgc accggcttcg agaagatcga ccgcgtgtgg ggtgaggtga
     2341 ccaatgtgct ggccgacacc attcctggca tccgggtggt caaggccttt gcccaggagc
     2401 accgtgaggc caagcgcttc catgacgcca acaagcacaa cctggtggtg aacgaccgta
     2461 tcaacaagat ctggtcgctg ttctcaccca gcgtgtcgct gctgaccgaa atcggtctgc
     2521 tggtggtgtg gggctttggc atctggcagg tggcccaggg cgagatcacc gtgggtatgc
     2581 tgctggcagc cattgcctac attggccgtt tctacggccg gctcgattcc atgagccgca
     2641 tcgtttccat gacgcagaaa tcggcctcgg cggccaagcg tatttttgac attctggacc
     2701 acgtttccag cgttccagag ccggtcaatc cggtgcccat gggcaaggtc gaggggcgca
     2761 ttgaagtgcg ggaagcaggt ttccgctatg gcaaccggga agtgaacaaa ggcatcaacc
     2821 tgagcattgc ccccggggaa atgatcggcc tggtcgggca cagtggctcg ggcaagagcg
     2881 cgctggttaa cctgatctgc cgcttctacg acgtatccga aggggccatt ctggtcgatg
     2941 gcgtcgacat ccgctcctac gccattgcgg atttccgccg gaacatcggc ctggtgctgc
     3001 aggagccttt cctgtttttt ggcaccattg ccgagaacat cgcctacggc aagcctgacg
     3061 ccacgcgcgg cgagatcatt gctgcggcgc gtgcggcgca tgcccacgag ttcatcctgc
     3121 ggctgccgca gggctacgac tcgatggtgg gggagcgcgg acagggactc tctggcggcg
     3181 agcgccagcg catttccatc gcccgtgcgc tgctgatcga cccccgcatc ctgatcctcg
     3241 atgaagccac atcgtcggtg gattccgaga ccgaaaagga aatccagaaa gcgctggaaa
     3301 acctggtgca aggccgcacg accatcgcca ttgcccaccg cctgtccacc ctgcaccgcg
     3361 ccgaccggct ggtggtactg gaccgtggcc gggtggtcga ggagggcacg cacgatgccc
     3421 tgatggccca ggagggcgcc tactacgcgc tgtaccaggc gcaggcccga aatatggacg
     3481 ttgacatgga cgacgcacga tgagcacctt gaaaaccgtt accaccacac gttttgcgct
     3541 ggaacgcaac gcctttggca aactgatcct gaccgatgcc gctggtgagc ggtttgaggg
     3601 tgtggtgccg gtgcgggcgt ttcccatcca gtcgcctgaa gatggcattg cactagtgag
     3661 taccgacgga catgaagtag gctgggtgga tggcctggag gaactcccgg gaccggttca
     3721 ggacctgatc cgccaggagt tgacatcgcg ggaattcatg ccagtgctgg agaggatcgt
     3781 ggatgtgtcg agcttctcca cgccctgcac ctggaccgtt gtcaccgacc ggggcgaaac
     3841 cgatttcgtg ctgcgggggg atgaagacat ccgccgcatc ggcaaagcgg gcagcctgat
     3901 gattgccgat acccacggca tccagtactt tgtacgcgac cagtatgcgc tggatgcgca
     3961 cagcaagaag attctcgacc ggtttatgta ggccctggcc gacccggcca gggtggtggg
     4021 gtcagaaccg gtaccggtat cccaccgtca aaaagtcttc accgttggca ccatagttgt
     4081 tcagcaggtc gtagaccgcg cagcggtggt gcagccgcac aaagaactcc tgttggggcg
     4141 actggggtgc agagaatgcg gcctccacaa acaggtagtt cagggtgcga gttccctgtc
     4201 cgtcggggcc gcgctccagc gggccgacgt ggtcgtggtc aaccccatcc gcgacgggct
     4261 c
//
LOCUS       DAOSJM010000207         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18386_length_4246_cov_3.438797, whole genome shotgun sequence.
ACCESSION   DAOSJM010000207 DAOSJM010000000
VERSION     DAOSJM010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18386_length_4246_cov_3.438797"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="PKX82_08175"
     CDS             <1..122
                     /locus_tag="PKX82_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HOF52271.1"
                     /translation="IPEISGNRLMVSIRLMRHDSDDRLHASGDDGAFELTLCS"
     gene            129..344
                     /locus_tag="PKX82_08180"
     CDS             129..344
                     /locus_tag="PKX82_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOF52272.1"
                     /translation="MGSVTNPTHRVRIVRCPACGGDSIYSSTNTYRPFCSERCKNMDL
                     GAWASESFRLPTEAPPDDQPFGDAKLQ"
     gene            complement(375..860)
                     /locus_tag="PKX82_08185"
     CDS             complement(375..860)
                     /locus_tag="PKX82_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOF52273.1"
                     /translation="MPAQPIVADPHTERVGGSQRSAVDVAVGVLVRPDGAFLLTSRPV
                     GKPYAGYWEFPGGKLEAGESVEQALRRELIEELGITIGPVHPWKMEMVDYPHALVRLH
                     FCKVFAWSGELQMREAQTHAWQQLPVTVQPVLPGTVPVLDWFAQERHHQGATYLIAAG
                     A"
     gene            complement(743..1735)
                     /locus_tag="PKX82_08190"
     CDS             complement(743..1735)
                     /locus_tag="PKX82_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF52274.1"
                     /translation="MDERLVQFLARAEAFMARVEGSLPHGLEQPDWNASIAFRYRRRS
                     SGQGVISAVRHVGSMALSDLKEIDAQKEKIQRNTAQFVSGAAANNVLLTGARGTGKSS
                     LIRACLHTYASQGLRLIEVDKADLVDLPDIVELVGQRPEKFIVFCDDLSFDEGESGYK
                     ALKSVLDGSVAAATPNVLIYATSNRRHLLPEYMSENLTYTHTQDGEVHPGEAVEEKIS
                     LSERFGLWVSFYPFNQEEYLQIVAQWLAALGVSQERMVAARPEALVWALERGSRSGRV
                     ALQFSRDFAGRHQSLPCLRSPSLPTRIPNAWAVRSAAPSTWLWVCWCGRMVHSC"
     gene            complement(1755..2984)
                     /gene="argJ"
                     /locus_tag="PKX82_08195"
     CDS             complement(1755..2984)
                     /gene="argJ"
                     /locus_tag="PKX82_08195"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOF52275.1"
                     /translation="MPVNLSLPDAATLFPIAGVRIGVTEAGVRKVGRKDVTVVLMDAG
                     ASVSGVFTSNRFCAAPVQICREHLTAGQGIRAMLINTGNANAGTGAEGLARARSTCEA
                     LAQKLDISVDQILPFSTGVIMEPLPGDRIIAGLDAAIADAQPGNWLRAAEGIMTTDTV
                     PKAFGKQVQIGGKTISITGISKGAGMIRPNMATMLGFIATDACVGQSVMKQLSMELAE
                     GSFNRVTVDGDTSTNDSLVVIASNKAGNAAVDSLDSADGKVLKAAMLDVARQLAQAIV
                     RDGEGATKFITVRVEGGRNAEECRKVAYAIAHSPLVKTAFYASDPNLGRILAAVGYAG
                     IDDLDQGGIELHLDDVHVATQGGRNPAYREEDGQRVMKQSEITVRVGLGRGAASDTVW
                     TCDFSHEYVTINADYRS"
     gene            complement(2995..>4246)
                     /locus_tag="PKX82_08200"
     CDS             complement(2995..>4246)
                     /locus_tag="PKX82_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOF52276.1"
                     /translation="KMITIATNMAGRGTDIVLGGNVEKSIQAVEVDDTLDETAKQLKI
                     EELRAQWAKDHEQIKALGGLRIIATERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDD
                     ALMRIFAGDRVRAIMDRLKMPEGEAIEAGIVTRSIESAQRKVEARNFDMRKQLLEYDD
                     VSNDQRKVIYQQRNAILDAQDLSAQISSLREGCFQDLVRQYVPAESVEEQWDIAALQK
                     ILADDWQIDLPLQKQVSEASAITDDDLLTTVIEAANASFQSKLDQVGAEHFTQFERMV
                     LLQSIDTHWRDHLSALDYLRQGIHLRGYAQKQPKQEYKREAFELFGQLLDSVKNEVTK
                     VLMTVKIQSNEQLDQAAEDMEARGESIANVTYSAPTETGEVETMVDKSTVQANASDVP
                     RVGRNDPCPCGSGKKYKQCHGKLA"
BASE COUNT          854 a         1308 c         1185 g          899 t
ORIGIN      
        1 tgatcccgga aatcagtggc aaccgcctca tggtgtccat ccggttgatg cgccacgact
       61 ccgatgaccg cctgcatgcc agtggcgatg atggtgcgtt cgaattgact ctgtgctctt
      121 gaactgaagt gggttcagtg accaatccaa cccacagagt ccggatcgtg cgttgtcccg
      181 cgtgtggtgg cgacagcatc tactccagta ccaataccta tcgcccattc tgcagtgaac
      241 gctgcaagaa catggacctg ggtgcatggg ccagcgagtc gttcaggttg ccaactgagg
      301 caccacctga tgaccagccg tttggcgatg ccaagctgca ataaataagg aaatctggct
      361 ctagcccccg tcgatcaagc gccagcagct attaaatatg tagcaccttg gtgatggcgc
      421 tcctgtgcaa accaatccag cacaggcacc gtaccaggca ataccggctg caccgtgacc
      481 ggcagttgct gccaggcatg cgtttgtgcc tcgcgcatct gcaactcgcc ggaccaggca
      541 aagaccttgc agaaatgcaa acgcaccagt gcatggggat agtccaccat ctccatcttc
      601 cagggatgga ccggtccaat cgtgataccc agttcctcga tcagttcgcg ccgaagtgcc
      661 tgctccaccg attcaccggc ctccagtttt ccgccaggga actcccaata gccagcgtac
      721 ggcttgccaa cggggcgtga ggtcagcagg aatgcaccat ccggccgcac cagcacaccc
      781 acagccacgt cgacggcgct gcgctgcgaa ccgcccacgc gttcggtatg cgggtcggca
      841 acgatgggct gcgcaggcat ggcaaggact ggtggcgccc tgcgaagtcg cgcgaaaatt
      901 gcaaggccac ccgcccactg cgcgaacccc gctccagagc ccacaccagt gcttcaggcc
      961 tggcagcaac catccgctcc tgcgatacgc ccagcgcagc aagccattgc gccacgatct
     1021 ggagatactc ttcctggttg aaggggtaaa aactcaccca caagccgaag cgctcggaca
     1081 acgaaatttt ttcttccacc gcttcaccgg ggtggacctc accgtcctgg gtgtgggtat
     1141 aggtcaggtt ttccgacatg tactcgggca gcaggtgacg ccggttgctg gtggcataga
     1201 tcagcacatt gggcgtggcg gcagccacag aaccgtccag caccgatttc agtgccttgt
     1261 agccggactc gccttcgtca aagctcaagt catcgcagaa cacgatgaac ttctcggggc
     1321 gctgccctac cagttccacg atatctggca ggtctaccag atccgccttg tcaacctcta
     1381 tcagccgcag gccttgcgat gcataggtgt gcaggcaggc gcggatgagc gaggatttgc
     1441 cggttccacg cgccccggtg agaagcacgt tgttggccgc agcaccactg acaaactggg
     1501 ccgtattgcg ctggattttt tctttctggg cgtcgatctc tttgagatcg gacaatgcca
     1561 tcgatcccac gtggcgcacc gcagagatca caccctgacc ggacgaacgc ctgcggtaac
     1621 gaaaagcaat ggaggcattc cagtcgggct gctccagacc atgtggaagg gatccttcca
     1681 cgcgcgccat gaaggcttcg gcacgtgcca gaaactgaac cagccgttcg tccatggatg
     1741 ctcccgattc tgaatcagga gcgatagtcg gcattgatcg tgacgtattc gtggctgaag
     1801 tcacaagtcc acaccgtgtc agaggctgca ccacggccaa gtcccacacg cacagtgatt
     1861 tcgctttgct tcatgacacg ctggccgtct tcttcccggt aggcaggatt gcgtccaccc
     1921 tgcgtggcca catgcacatc gtcgagatgc aattcaatac cgccctgatc gaggtcatca
     1981 atgccagcgt agcccaccgc ggccagaatg cgtccgaggt tggggtcgct ggcataaaac
     2041 gcagttttga ccagcggaga gtgcgcaatg gcataggcca ccttgcgaca ctcttctgcg
     2101 ttacgtccgc cctcgacacg tactgttatg aatttggtgg caccctcgcc atcgcgcacg
     2161 atggcttgcg ccagctggcg ggcaacgtcg agcatcgccg ctttgagcac tttgccatct
     2221 gcactgtcga gagaatcaac ggcagcattg cctgccttgt tggaagcgat gaccaccagg
     2281 gagtcattgg tggaggtgtc gccgtccacc gtcacccggt tgaaggatcc ctccgccaat
     2341 tccattgaca gctgcttcat cactgactgg cccacgcagg cgtcggtggc aatgaatccc
     2401 agcatggtcg ccatgttggg gcggatcatg cccgcgccct tgctgatgcc ggtgatgctg
     2461 atggttttcc caccgatctg cacctgcttg ccgaaggcct tgggaaccgt gtcggtagtc
     2521 atgatgcctt ccgcagcacg caaccagtta ccgggctgcg catccgcaat ggcggcatcc
     2581 agccccgcga tgatgcggtc accaggcagc ggctccatga tgacgccggt cgaaaacggc
     2641 agtatctggt ctaccgatat gtccagtttc tgggcaagcg cttcgcaggt gcttcgtgca
     2701 cgggccagac cctccgcacc ggtaccagca ttggcattgc ccgtgttgat caacatggcg
     2761 cgaataccct gtccagcggt gagatgctcc cggcaaatct gcacaggtgc cgcacaaaac
     2821 cggttgctgg taaagacgcc cgacacgctg gcgcccgcgt ccatcagcac gacggtgaca
     2881 tccttgcggc caactttgcg cacaccggct tcggtaaccc caatacgcac accggcgatc
     2941 gggaacagcg tcgctgcatc aggaagagaa aggtttacag gcatagtgca gtcttcaggc
     3001 caatttgccg tggcattgtt tgtatttctt gccgctgccg caagggcacg gatcgttacg
     3061 gccgacacgg ggcacgtcgc tggcgttggc ttgcaccgtg gacttgtcca ccatggtttc
     3121 tacttcacct gtttcggtag gcgcactgta ggtcacgttg gcgatgcttt ctccccgggc
     3181 ttccatatct tcggcggcct gatccagctg ttcattcgac tggattttta ctgtcatgag
     3241 caccttggtc acttcattct tgaccgaatc cagtaactga ccaaacagct caaacgcttc
     3301 acgcttgtac tcctgcttgg gctgcttctg ggcgtaaccg cgcagatgga tgccctggcg
     3361 caggtaatcc agtgcactca ggtgatcacg ccaatgggta tcaatgcttt ggagcaagac
     3421 catgcgctcg aactgcgtga aatgctcggc tcccacctgg tccagtttgg actggaacga
     3481 cgcgttggct gcctcaatca cagttgtgag caagtcgtca tcggtgatgg cgctggcttc
     3541 gctcacctgc ttttgcaggg gcaggtcgat ttgccagtca tcggccaata tcttctgcag
     3601 ggctgcaatg tcccattgct cttcgacgga ctcagcgggc acatactggc gcaccagatc
     3661 ctggaagcaa ccttcacgca gagaggaaat ctgcgcactc aggtcctgcg catccagaat
     3721 ggcattgcgc tgctggtaga tcaccttgcg ctggtcattg gatacatcgt catactccag
     3781 cagctgcttg cgcatgtcga agttgcgcgc ttcgaccttg cgctgggcgc tctcgatgct
     3841 gcgggtcacg atacctgcct caatggcctc gccttcgggc atcttcaggc ggtccatgat
     3901 ggcccggaca cggtcgccag cgaagatgcg catcagtgca tcgtccaggc tcaggtaaaa
     3961 gcgcgaggag ccgggatcgc cctggcgtcc ggaacggcca cgcaactggt tgtcaatacg
     4021 gcgggactca tggcgttcgg tcgcaatgat gcgcaaacca ccaagggcct tgatctgctc
     4081 gtggtctttt gcccactgcg cacgcaattc ttcaattttt agctgcttcg cagtctcatc
     4141 cagcgtgtca tccacctcga ctgcctggat ggacttctca acgttgccac caagcacaat
     4201 gtcggtaccg cgaccggcca tattggtggc aatggtaatc atcttg
//
LOCUS       DAOSJM010000208         4221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18522_length_4221_cov_4.991359, whole genome shotgun sequence.
ACCESSION   DAOSJM010000208 DAOSJM010000000
VERSION     DAOSJM010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18522_length_4221_cov_4.991359"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="PKX82_08205"
     CDS             complement(<1..496)
                     /locus_tag="PKX82_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF52277.1"
                     /translation="MSWYHPADVGLAVRSKMADLGYGARLFGRLLASLGPVLRRPGLL
                     RDQVHFMGNYSLAIIAVSGVFVGFVMGLQYYYGLQRFGAAESVGMLISLSLVREFGPV
                     LTALLFAGRAGTSLTAEIGLMKAGEQLSAMEMMAVDPVSRILAPRFWGAVIAMPLLAA
                     VFSAV"
     gene            complement(493..1332)
                     /locus_tag="PKX82_08210"
     CDS             complement(493..1332)
                     /locus_tag="PKX82_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF52278.1"
                     /translation="MADYSTDPLVELKDLSFGYGERRILDGVTLSIPRGKVTALMGAS
                     GGGKTTVLRLIGGQYRAQAGQVLLHGAQYGKDVQDMGKANAQALYAARRRMGMLFQFG
                     ALFTDLSVFDNVAFPLREHTNLSPALVRDVVLMKLNAVGLRGARDLMPSEVSGGMARR
                     VALARAIALDPELVMYDEPFAGLDPISLGTAARLIRQLNDTMGLTSIVVSHDLEETFH
                     IADKVIVLANGKIAAQGTPQEVRESTDPLVHQFVNALPEGPVRFHYPASSVDADFSMG
                     AGR"
     gene            complement(1391..2857)
                     /locus_tag="PKX82_08215"
     CDS             complement(1391..2857)
                     /locus_tag="PKX82_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOF52279.1"
                     /translation="MGKITGFMEFERIEEGYKPVPERLKHYKEFVVGLDDAQSNKQAA
                     RCMDCGTPFCNSGCPVNNIIPDFNDLVYRGDWKAAIEVLHSTNNFPEFTGRICPAPCE
                     AACVVNVNGDAVGIKSIEHAIIDKAWSEGWVKPLPAKVKTGKKVAVVGSGPAGMAAAQ
                     QLARAGHDVTLFEKNDRIGGLLRYGIPDFKMEKSHIDRRADQLKAEGVTIRTGVMVGE
                     LPKDSKVTNWAKESISPAQLQKDFDAVLLTGGSEQSRDLPVPGRDLDGIHFAMEFLPQ
                     QNKVNAGDKLKNQLRADGKHVIVIGGGDTGSDCVGTSNRHGAVSVTQFEVMPQPPEQE
                     DKPLVWPYWPLKLRTSSSHDEGCTREFAISTKEFVGKNGKVTGLKTVHVELKDGKLVE
                     IAGTEKEYKADLVLLAMGFVNPVATILDGFGVDKDGRGNAKASTEFTGGYATNVAKVF
                     AAGDMRRGQSLVVWAIREGRQAARSVDEFLMGSSDLPR"
     gene            complement(2870..>4221)
                     /locus_tag="PKX82_08220"
     CDS             complement(2870..>4221)
                     /locus_tag="PKX82_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOF52280.1"
                     /translation="SRIRVQADGQMKTGRDVAIGALLGADEFGFATAPLVVEGCIMMR
                     KCHLNTCPVGVATQDPVLRQKFSGKPEHVVNYFFFIAEEVRQIMAQLGIRKFDDLIGR
                     ADLLDTRKSIEHWKAQGLDFSRLFAQPQVPADVPRFQTLEQEHGLEKALDNVLIAKSR
                     AAIDKGERVQFMEVARNVNRSVGAMLSGAVTKVHPEGLPDDTIRIQLEGTGGQSFGAF
                     LAKGITMYLIGDANDYTGKGLSGGRIVVRPSIDFRGEAIRNTIVGNTVMYGATTGEAY
                     FSGVAGERFAVRLSGATAVVEGTGDHGCEYMTGGTVAVLGKTGRNFAAGMSGGVAYVY
                     DEDGQFAKRCNTAMVSLEKVLTTSEQEAAMDKAIWHRGESDEAQLKKLLEDHNRWTGS
                     KRARELLDNWDASRAKFVKVFPNEYKRALGEIHARKSAVAPVKPAQAATKKVATAAK"
BASE COUNT          861 a         1345 c         1201 g          814 t
ORIGIN      
        1 cgaccgcgct gaacaccgcc gccagcaagg gcatggcaat gaccgcaccc cagaaacggg
       61 gcgccagaat acgcgagaca ggatcaaccg ccatcatttc catggcactg agctgctcac
      121 ccgccttcat cagaccgatc tcagcggtga gcgacgtacc tgcccgaccg gcaaacaaca
      181 gggccgtcag cacggggcca aactcgcgca ccaggctcag gctgatgagc atgccaaccg
      241 actcggccgc accgaaacgc tgcaagccat agtagtactg cagacccatc acaaaaccga
      301 cgaacacacc ggacaccgca atgatggcca gcgaatagtt gcccatgaaa tgcacctggt
      361 cgcgtagcaa gccaggacgg cgcaacaccg gccccagcga agccagcaaa cgaccaaaca
      421 gccgggctcc atagcccaga tcggccatct tggaacgcac agccaggcca acgtcagcag
      481 ggtggtacca gctcatcgtc ctgcccccat ggaaaagtct gcatccacac tggaggcagg
      541 gtaatggaac cgcaccggac cttcgggcaa ggcgttgacg aactggtgca ccagcggatc
      601 ggtgctttcg cgcacctcct gcggcgtacc ctgggcggca atcttgccat tggccagaac
      661 aatcaccttg tcggcaatat ggaaggtttc ctccagatcg tgcgagacga cgatgctggt
      721 cagccccatg gtgtcattga gctggcgaat caggcgtgca gccgtcccca gggagatcgg
      781 atccagcccg gcgaagggtt cgtcgtacat caccagttca ggatccagcg caatggctcg
      841 ggccagggcc acacggcgtg ccataccacc agacacctcg gacggcatca ggtcgcgcgc
      901 accacgcagc cccacggcat tgagtttcat gagaacaacg tcgcgcacca gcgccgggga
      961 caaattggtg tgctcacgca aaggaaatgc cacattgtcg aacacgctga gatcggtgaa
     1021 cagggcccca aactggaaca gcatgcccat gcggcgacgc gcagcgtaga gggcctgtgc
     1081 attggctttg cccatatcct gcacatcttt gccgtactgc gcaccatgca acagcacctg
     1141 ccctgcttgc gcacggtact gtccaccaat caggcgcaac acggtcgttt ttccaccccc
     1201 cgacgcaccc atcaacgcgg tcaccttgcc gcgcggaatc gacagggtca cgccatcgag
     1261 gatccgccgt tcgccgtagc caaaggacag atctttcagt tcaacgagcg ggtcagtgga
     1321 atagtcggcc atacaaacaa caaggccggg gaaatacccg gcctcggaat gataagcggg
     1381 aaaaccctga tcagcgtggc aggtcgctag accccatcag gaactcgtct accgagcgcg
     1441 ctgcctgacg gccttcgcgg atagcccaca ccaccagact ttgaccacgg cgcatatcgc
     1501 cagcagcaaa caccttggcc acattggtgg catagccgcc ggtgaactcg gtcgatgcct
     1561 tggcgttgcc gcggccatcc ttgtccacgc caaagccgtc cagaatggtc gcaacggggt
     1621 tcacaaagcc catggccagc agcaccaagt cggccttgta ttccttttcg gtaccggcaa
     1681 tttccaccag cttaccgtcc ttgagctcga catgcacggt tttcagccca gtgactttgc
     1741 cgttcttgcc gacaaattcc ttggtggaaa tggcaaattc gcgggtgcag ccctcgtcgt
     1801 ggctggaact ggtacgcagt ttcagcggcc agtaaggcca gaccaggggc ttgtcttcct
     1861 gctcgggagg ctggggcatc acctcgaact gggtcacgct gaccgcacca tggcggttac
     1921 tggtacccac gcagtccgag ccggtgtcac caccgccaat gacgatcaca tgcttgccgt
     1981 ccgcgcgcaa ctggttcttg agcttgtcgc ccgcgttgac cttgttctgc tggggcagaa
     2041 actccatggc aaagtgaatg ccatcaaggt cacggccagg caccggcaag tcgcgcgact
     2101 gctcagaacc gccagtcaac agcaccgcgt caaagtcttt ttgcagctgt gcgggggaga
     2161 tgctctcctt ggcccagttg gtcaccttgg aatccttggg caactcaccg accatcacac
     2221 cggtgcggat ggtgacaccc tcagccttga gctggtcggc gcggcggtcg atgtgcgact
     2281 tctccatctt gaagtcgggg ataccgtagc gcagcaggcc accgatacgg tcgttcttct
     2341 cgaacagggt cacatcgtgg ccagcccggg ccagttgctg cgcagcagcc atgccagcag
     2401 ggccggaacc gaccacagca acctttttgc cggttttcac cttggctggc aggggtttga
     2461 cccagccttc ggaccaggct ttgtcaatga tggcgtgttc gatggacttg atgcccaccg
     2521 catcaccatt gacattcacc acgcaggcag cttcgcaggg tgcggggcag atgcggccgg
     2581 tgaactcggg aaagttgttg gtgctatgca gcacctcaat cgcagccttc cagtcgcccc
     2641 ggtacaccag gtcgttgaaa tccggaatga tattgttgac cgggcagccg ctgttgcaaa
     2701 acggtgtacc gcagtccatg cagcgtgcag cctgtttgtt ggactgtgcg tcgtccagtc
     2761 cgaccacgaa ctctttgtag tgcttcagac gttctggaac gggcttgtag ccctcttcga
     2821 tgcgttcaaa ctccatgaag ccagtaattt ttcccatgat tctgttcctt tacttggctg
     2881 cagttgctac ttttttggta gctgcttgcg caggcttgac gggggctaca gccgattttc
     2941 gtgcatgaat ctcacccaga gcacgcttgt actcgttggg gaagaccttc acgaacttgg
     3001 cgcgcgaggc atcccagttg tccagcagtt cacgcgcacg cttgctgcca gtccagcgat
     3061 tgtggtcttc aagcaacttt ttgagctgcg cttcatcgct ctcgccacgg tgccaaatgg
     3121 ccttgtccat cgcggcttcc tgctcggagg ttgtcaacac cttctccagg ctcaccatgg
     3181 cagtattgca gcgcttggcg aactggccgt cctcgtcgta cacataggcc acgccaccgc
     3241 tcatacccgc cgcaaagttg cggccggttt tgccgagcac ggcgactgtt ccaccagtca
     3301 tgtattcgca accgtggtcg cccgtacctt ccaccaccgc agtggcgccg gacaggcgca
     3361 cagcgaagcg ctcaccagct acaccgctga agtaggcctc accggtcgtc gcaccgtaca
     3421 tcacggtgtt gccgacgatg gtgttgcgga tggcttcgcc gcggaagtcg atgctcggac
     3481 gcaccacaat gcggccacct gacagtccct tgccggtgta gtcgttggcg tcgccaatca
     3541 ggtacatggt gatgcccttg gccaggaagg caccaaagga ttgaccacca gtaccttcga
     3601 gctggatgcg gatggtgtca tccggcaaac cttcgggatg caccttggtc accgcaccgg
     3661 aaagcatggc acccacggaa cggttgacgt tgcgggccac ttccatgaac tgcacgcgct
     3721 cgcccttgtc gatggcagct cggctcttgg cgatcagaac gttgtccagc gccttttcca
     3781 gaccatgctc ctgctccagc gtctggaagc ggggcacatc ggcgggcact tgcggctggg
     3841 caaacaggcg gctgaagtcc agaccctggg ctttccagtg ctcaatgctc ttgcgggtat
     3901 caagcaggtc tgcgcggcca atcaggtcgt cgaacttgcg gatacccagc tgggccatga
     3961 tttggcggac ttcttcggcg atgaagaaga agtagttgac cacatgctcg ggcttgccgg
     4021 agaatttctg gcgcaacacc gggtcctgtg tcgccacgcc caccgggcag gtgttcaggt
     4081 ggcacttgcg catcatgatg cagccttcca ccaccagcgg tgcagtggca aagccgaatt
     4141 cgtcagctcc caacagcgcg ccgatagcga cgtcgcggcc ggtcttcatc tggccgtcgg
     4201 cctgcacgcg gatgcggctg c
//
LOCUS       DAOSJM010000209         4188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18718_length_4188_cov_4.071135, whole genome shotgun sequence.
ACCESSION   DAOSJM010000209 DAOSJM010000000
VERSION     DAOSJM010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18718_length_4188_cov_4.071135"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..790
                     /locus_tag="PKX82_08225"
     CDS             <1..790
                     /locus_tag="PKX82_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOF52281.1"
                     /translation="QRMDAGEVIDAKGQQGKAAAAFTPERRLYYYRTVEQEVPIPFEA
                     EVLHQDEHLLVVDKPHFLPVIPSGKYVQETVLVRLKHRLGLPDLSPIHRIDRDTAGLV
                     LFSVNPASRNAYQSLFRHRQVDKTYECIATWNPALPWPVRRESRIGPAEHFMQQTEVP
                     GAPNALTHIAPLEVHGSLARYTLKPVSGQRHQLRVHMAALGLPIVNDGIYPTLTPEGA
                     DYARPLQLLARAIAFTDPVSGAPRQLTSARSLLPLTDLLAVRPA"
     gene            complement(770..1810)
                     /locus_tag="PKX82_08230"
     CDS             complement(770..1810)
                     /locus_tag="PKX82_08230"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOF52282.1"
                     /translation="MTGGVVGRNLRIDFLKLLCAQCIVLHHFSVYGPVGDALAAAAPA
                     VSAWLYDYGRMAVQVFLVLGGYLAVGSLERMCATGSRPLVAVVWKRYLRLVLPLLAAL
                     LLVTLVAALTRLGLQDDFIPDAPSLSQVLSHVLLLQDVQGIEALSVGVWYVAIDFQLY
                     VLMAFLVWGGQALAAVPHATRVLVGALMLLSLFHANRNPDWDAWAVYFFGAYGMGAVA
                     RWAQRSPHRALMLAGLVAVVALALVMEFRERLVLALVTALALGTMPRTARVWPAGLQR
                     WVALLGQSSYALFLVHFSVLMLVNLVFAQWSPAGPWATGLALLAGVALSTGLAVVFAR
                     RVETPLSRWADR"
     gene            complement(1807..2982)
                     /locus_tag="PKX82_08235"
     CDS             complement(1807..2982)
                     /locus_tag="PKX82_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52283.1"
                     /translation="MSTTMTLANDGLQRTSPQPQGILIRQLACADGQEATRAVLEALD
                     AQDLMACVEAQWSAGAGGGGAFNLWLRPAQAAQWAPGLDTLDLASTLQLDTEASEKDL
                     LREICVAMLMGPVAFEFPSLEELVSAVRIRRNIVQAARKTTLAFHTSEAERPEDCWTY
                     DEDRGFVIKPGASLIDAMTKATQPEVSGALYSFSCYRATEYVIVLGIGQELVQCNPPL
                     YQQLQTLWEQRPIRSGEFHDVFLREQGSMEEPLPPRYYVPGDRTWFRNPDGVSAEASG
                     FEGSWVMYMGGGLFTNFWKRDQPYTLEQKCLEIYHWRHGLYFDAEGEERIDEDKVAVL
                     VQETLRNPAEAARIRALTERYREARGIYTDAGGCIDTTREFARWVCPGTADMVLPLP"
     gene            complement(2979..4082)
                     /locus_tag="PKX82_08240"
     CDS             complement(2979..4082)
                     /locus_tag="PKX82_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HOF52284.1"
                     /translation="MTFQRLANSIVTRLILPGFLLLGIALPGVSAGQVAHRWISVPRI
                     SGHLGPADLGVVINTADPYSVEVGEYYVRKRGIPPEQVLRVELPVRNALSVAEFGALY
                     AQIRDSMGPQVQALALVWTQPFAVGCNAITSAITLGLEPEACRNGCAVSRPSRYFNAP
                     TARPFTDLGLRPSMLLASRSVESARALIDRGVASDGTLGKLGGPAANAVFVTTRDTAR
                     SVRSPLFPPAGRVSKLGVQVVLRQQGDSTPLRRVILYQTGVSREAAIDSQQWLPGALA
                     DHLTSYGGQLTNVQGQMSVLEWLESGATASYGTVSEPCNHLQKFPHPQVLLLNYVQGA
                     TALEAYWRSVAWPAQGVFVGEPLAAPFHPLNPP"
BASE COUNT          845 a         1447 c         1249 g          647 t
ORIGIN      
        1 ccagcgcatg gacgcgggcg aggtgatcga tgccaaaggc cagcagggga aggctgctgc
       61 cgcgttcacg ccagagcggc gtctgtacta ctaccgcacc gtagagcagg aagtgcccat
      121 cccctttgag gccgaagtgc tccaccagga cgagcatctg ctggtggtgg acaagccgca
      181 ctttctgccg gtgattccct caggcaagta cgtgcaggag accgtgctgg tgcgcctcaa
      241 acaccgcctg ggtctgccgg acctgtcccc catccaccgc attgaccgcg ataccgccgg
      301 actggtgctg ttctcggtca accctgcttc ccgcaacgcc taccagtccc tgttccggca
      361 tcgccaagtc gacaagacct acgagtgcat tgccacgtgg aatcctgcgc tgccctggcc
      421 cgtgcgacgc gaaagccgca tcggtccggc agagcatttc atgcagcaaa ccgaggttcc
      481 gggcgcgccc aatgccctga cccacatcgc accgctggaa gtgcacggca gcctggcccg
      541 ttacaccctc aagccagtca gtggccagcg ccaccagttg cgggtgcaca tggcagcgct
      601 gggcttgccc atcgtgaacg atgggattta ccccaccctc acccccgagg gcgcggacta
      661 cgccaggcca ctgcagctgc tggcgcgtgc cattgcgttt accgatccgg tgagcggtgc
      721 accccgccag ctcaccagcg cgcgcagcct gttgccactg accgacctgc tagcggtccg
      781 cccagcgtga caaaggcgtt tccacgcggc gtgcaaacac caccgccagc ccggtactga
      841 gcgcaacacc cgccagcaaa gccagccccg ttgcccacgg ccccgccgga ctccactgtg
      901 caaacaccag attgaccagc atcagcaccg aaaagtgcac cagaaacagt gcatacgacg
      961 actgccccag cagcgccacc cagcgttgca gccctgcggg ccaaacgcgg gcggtgcgtg
     1021 gcatggtccc cagagccagt gccgtgacca gtgccagcac cagccgctcg cgaaactcca
     1081 tcaccagggc cagtgcgact acggccacca gtcccgccag catcagcgca cggtgcggcg
     1141 agcgctgggc ccagcgggcg acagcgccca tgccataggc accaaaaaaa tacaccgccc
     1201 aggcgtccca gtccgggttg cggttggcat gaaaaagcga gaggagcatc agcgcgccta
     1261 ccaggacccg ggtggcgtgg ggcacggcag ccagtgcctg accaccccac accaggaagg
     1321 ccatcaacac atacaactgg aaatcaatcg ccacatacca gacgcccacg gacaacgcct
     1381 caatgccctg cacatcctgc aacagcagta catggctcag cacctgcgaa agcgaaggcg
     1441 cgtcgggaat gaaatcgtcc tgcaacccta gccgggtcag tgcagcaacc agggtgacga
     1501 gcagcagcgc agccagcagc ggcaggacca ggcgcaggta gcgcttccac accactgcca
     1561 ccagtggcct cgaaccggta gcacacatgc gctccaggct gcccaccgcc aggtagccac
     1621 ccagtaccag aaacacctga accgccatgc ggccgtagtc gtacagccag gcggacacgg
     1681 ccggggcagc agccgccagc gcatccccga ccggcccgta gaccgagaag tggtgcaaca
     1741 caatgcactg ggcacacagc agcttgagaa agtcaatgcg cagattgcgc cccaccaccc
     1801 cacccgtcat ggcaagggaa gcaccatgtc cgccgtaccc ggacacaccc agcgtgcgaa
     1861 ctcccgcgtg gtgtcgatac agccaccggc atcggtgtaa atgccacgcg cctcgcggta
     1921 gcgctcggtc agcgcacgga tacgggcggc ctcggcaggg ttgcgcagcg tctcctgcac
     1981 cagcaccgcg accttgtcct cgtcaatgcg ctcctcgcct tcggcgtcga agtagaggcc
     2041 atggcgccag tggtagattt ccaggcactt ctgctccagg gtatagggct ggtcgcgttt
     2101 ccagaagttg gtaaacaagc cgccgcccat gtacatcacc cacgagccct caaaacccga
     2161 ggcttcggcc gacacgccat cgggattgcg aaaccaggtg cggtcgcccg gcacgtagta
     2221 gcgtggcggc agcggctctt ccatcgaacc ctgctcacgc aggaatacat cgtgaaattc
     2281 gccagaccgg atcgggcgct gttcccacag cgtctgcagc tgctggtaca gcggggggtt
     2341 gcattgcacg agctcctggc caatgccgag cacgataacg tactcggtgg cgcggtaaca
     2401 ggagaacgaa tacaacgccc cggacacttc gggttgcgtg gccttggtca tcgcgtcgat
     2461 cagcgaggct cctggcttga tgacaaagcc gcggtcttcg tcgtaggtcc agcagtcttc
     2521 cggtcgttcg gcctcgctgg tgtgaaatgc cagcgtggtc ttgcgcgcgg cttgcacgat
     2581 attgcggcga atgcgcacgg cagacaccag ctcctccagg ctggggaact caaacgccac
     2641 cggccccatg agcatggcga cacagatttc ccgcagcagg tccttttcgc tggcctcggt
     2701 gtcgagctgc aaggtgctgg cgagatccag cgtatccagc ccaggagccc attgcgcggc
     2761 ttgggccgga cgcagccaca ggttgaatgc accaccaccc cctgcacctg cagaccattg
     2821 cgcttcaaca caggccatca ggtcctgtgc atccagtgct tccagcacgg cccgcgtggc
     2881 ctcctgcccg tctgcacacg ccagctggcg gatcagaatg ccctggggtt gcggggaggt
     2941 cctctggaga ccgtcgttgg caagcgtcat cgttgtgctc acggtggatt caatggatgg
     3001 aacggtgcag ccagtggctc acccacaaat acgccctgcg ccggccaggc cacgctgcgc
     3061 cagtaggcct ccagcgccgt tgcgccctgc acatagttca gcagcaggac ctggggatgc
     3121 ggaaatttct gcaggtggtt acacggttcg ctcacagtcc cgtagctggc ggtggcaccg
     3181 gactccagcc actccagcac actcatctgg ccctgcacgt tcgtcagttg cccaccatac
     3241 gaagtgagat ggtcggccag agcacccggc agccactgct ggctgtctat ggcggcctct
     3301 cgcgacacgc ccgtctggta caggatcacc cgtcgcagcg gcgtggaatc cccttgctgc
     3361 cgcagtacca cctgcacacc gagcttgctc acccggccgg ccggtggaaa caggggcgat
     3421 cgcacactgc gtgcggtatc ccgggtggtg acaaagaccg cgttggcggc cgggccgccg
     3481 agcttgccca gcgtaccatc cgacgccacg ccacggtcaa tcagcgccct ggcactctcc
     3541 accgagcggg aagccagcag catggacggg cgcagcccca gatcggtgaa cggccgcgcc
     3601 gtgggcgcat tgaaatagcg cgaaggcctg ctcaccgcac agccattgcg gcaggcctcg
     3661 ggctccagcc ccagcgtaat ggcagacgtg atcgcgttgc agcccacggc aaatggctgg
     3721 gtccagacca gcgccagggc ctgcacctgg ggccccatgg aatcgcgaat ctgggcatac
     3781 aaggccccga actccgccac actgagcgca ttgcgcaccg gcagttctac ccgcaacacc
     3841 tgctctggcg gaatgccacg cttgcgcaca tagtattcac cgacttccac ggaatacggg
     3901 tctgccgtgt tgatgaccac gcccagatcc gcagggccca gatgcccaga aatgcgaggc
     3961 accgatatcc agcggtgggc tacttgcccg gccgataccc ctggcagtgc aatgcccaaa
     4021 agaaggaaac ccggcaggat gagccgggtg acgatgctgt tggcaaggcg ttgaaacgtc
     4081 attgccttca tgatagacga caaggccgtc tatcgcggga aggctcaggc agtgatttcc
     4141 ctagcgctga tctggtactt gaagtcgtcc ggcgcgtagg agtccagc
//
LOCUS       DAOSJM010000210         4187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18725_length_4187_cov_3.770571, whole genome shotgun sequence.
ACCESSION   DAOSJM010000210 DAOSJM010000000
VERSION     DAOSJM010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18725_length_4187_cov_3.770571"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="PKX82_08245"
     CDS             complement(<1..1191)
                     /locus_tag="PKX82_08245"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOF52285.1"
                     /translation="MTKFVFVTGGVVSSLGKGIASASLAAILESRGLTVTLIKLDPYL
                     NVDPGTMSPLQHGEVFVTDDGAETDLDLGHYERFIETRMRKTNNFTTGQIYKSVLEKE
                     RRGDYLGKTVQVIPHVTNEIQEFVKRGARFGEPDAVDVAIVEIGGTVGDIESLPFLEA
                     VRQMSLKLGPNNSAFVHLSYLPWIAAAGELKTKPTQHTAKQLREIGIQADVLLCRADR
                     PIPDEERAKISLFSNVPEWGVISMWDVDTIYKVPRMLHEQGLDGLICDKLRLNTPPAS
                     LKRWDELVYETEHPQGEVTIAMVGKYVDLSDSYKSVNEALRHAGMKNHVRVKIEHVDS
                     ETITSDSVSQLAKYDAILVPGGFGKRGIEGKISTARFARENKVPYLGICLGMQVATIE
                     FARHV"
     gene            1262..2485
                     /gene="coaBC"
                     /locus_tag="PKX82_08250"
     CDS             1262..2485
                     /gene="coaBC"
                     /locus_tag="PKX82_08250"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOF52286.1"
                     /translation="MNDLAGKHIVLGLTGGIACYKAAELCRALIKEGATVQVVMTEAA
                     AKFITPVTMQALSNRSVYDSQWDAREGNNMAHINLSREADAILIAPCSADFMAKLLHG
                     RADDLLSLMCLARPIDKVPLLIAPAMNREMWAHPATQRNLAQLKADGTTVFDVGTGDQ
                     ACGETGDGRMLEPEELLQDLVSFFLPKVLAGQHVLVSAGPTYEAIDPVRGITNLSSGK
                     MGFAIARAAHEAGARVTLVAGPVSLPTPRGVERVNVRSAQNMLEASAEYAQDATVFIA
                     TAAVADWRPAQASDQKIKKDGSGATPQLDFVENPDILATVAKTPRAAAGALYCVGFAA
                     ESHDLLVHATAKRARKGVPLLVGNIGPATFGQDDNALLLVDAHGAQEIPRNSKLVLAR
                     HLVTEIARRLRATQP"
     gene            2491..2937
                     /gene="dut"
                     /locus_tag="PKX82_08255"
     CDS             2491..2937
                     /gene="dut"
                     /locus_tag="PKX82_08255"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOF52287.1"
                     /translation="MKIDVKILDQRLADNLPTYATPGSAGLDLRACLDAPLTLAPNAW
                     QLVPTGMAIYLHDPAYAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSAWNRSS
                     TEFTIEPMERIAQLVIVPVVQAQFNIVSEFPASQRGEGGYGSTGKA"
     gene            complement(2934..3467)
                     /locus_tag="PKX82_08260"
     CDS             complement(2934..3467)
                     /locus_tag="PKX82_08260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOF52288.1"
                     /translation="MEITQQCVVALTWTLKDTLGEILDVLDEPIEFLISGQDLLPAIE
                     EALQGHGAGATVQLQIEPEQAFGDFNDQLIFLEPRGLFPAELEEGLTMEGSALPAGCN
                     PDAPKDTLYTVTDIYPEHVVLDGNHPLAGIAIRITIKVESVREATEEEISRGSAGSGF
                     FRIQPFHESGPGSDTLH"
     gene            3485..>4187
                     /locus_tag="PKX82_08265"
     CDS             3485..>4187
                     /locus_tag="PKX82_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF52289.1"
                     /translation="MNIQEPLQLLGGLSPQVFMQRHWEKKPLLIRQAIPGFQPILDRT
                     ELLDMAAMDDVESRLVVQAVPGGQKAWKFRHGPFPRKSLPPFKQRAWTVLVQGMDLHD
                     ERVHALMNQFRFVPDARLDDLMISYATDGGGVGPHFDSYDVFLLQAHGRRRWRIGRQK
                     DMSLLPDMPLKILANFEPEMEFVLEPGDMLYLPPRWAHDGIAEGECMTYSIGFRSPNM
                     GEVAREVLQRMAEQAD"
BASE COUNT          809 a         1213 c         1284 g          881 t
ORIGIN      
        1 tacgtggcgt gcaaattcga tggtagcaac ctgcatgccc aagcaaattc ccagataggg
       61 caccttgttt tcgcgggcaa aacgcgctgt gctgatcttg ccttcaatgc cgcgcttgcc
      121 aaagccgccg ggcaccagaa tggcatcgta cttggcaagt tggctcacgc tgtcggaggt
      181 gatggtttcc gagtccacgt gctcaatctt gacgcgcaca tggttcttca tgcctgcgtg
      241 gcgcagagcc tcattgaccg acttgtagct gtcagagagg tccacatact tgcccaccat
      301 ggcaatggtg acttcacctt gtgggtgctc ggtttcatac accagctcat cccagcgctt
      361 caggcttgct ggaggggtat tcagacgcag cttgtcgcag atcagaccat ccagaccctg
      421 ctcatgcagc atgcgcggca ccttgtagat ggtgtccacg tcccacatgg aaatgacgcc
      481 ccattcgggc acattggaga acagtgaaat tttggcgcgc tcttcgtcgg ggatcgggcg
      541 gtcggcacgg cacagcagca cgtcggcctg gatgccgatc tcgcgcagct gcttggcggt
      601 gtgctgggtc ggcttggtct tgagttcacc ggctgcagca atccagggca ggtagctcaa
      661 gtgcacgaat gcactgttgt tggggccgag cttgagactc atctggcgta cggcttcgag
      721 gaatggcagg gactcgatgt cgcccacggt gccaccgatt tccacgatgg ccacatccac
      781 cgcatcgggt tcgccaaagc gtgcaccgcg tttgacgaac tcctgtatct cgttggtgac
      841 gtgggggatg acctggacgg tcttgcccag gtagtcgccc cggcgctcct tctcgagcac
      901 gctcttgtag atctggccgg tggtgaagtt gttggttttg cgcatgcgcg tttcaatgaa
      961 acgctcgtaa tggcccaagt ccaggtcggt ttctgcgccg tcgtctgtca caaatacttc
     1021 gccgtgctgc aggggcgaca tggtgccggg gtctacgttg aggtaggggt ccagcttgat
     1081 gagggtgact gtcaggcccc gcgattccag gatcgcagcg agggaggctg aggcgattcc
     1141 cttgccaagg gaggacacca caccgccggt gacgaagaca aatttggtca tgtcaggggg
     1201 agctagttac gctcggggag gtggtaaagc gagattatag aggtctgcta cattgccacc
     1261 catgaatgac ctcgctggta aacatattgt cctgggcttg acgggtggca ttgcctgcta
     1321 caaggcggct gaattgtgtc gggctctgat caaggaaggt gccacggtgc aggtggtgat
     1381 gaccgaggct gcggcaaagt tcattactcc ggtgaccatg caggcattga gcaaccgttc
     1441 cgtgtacgac tcgcagtggg acgcgcgcga aggcaacaac atggcgcata tcaaccttag
     1501 ccgcgaggcc gatgccatcc tgattgcccc ttgtagcgca gatttcatgg ccaagctgct
     1561 gcatggccgt gctgacgatc tgctgagtct catgtgtctg gcccgaccca tcgacaaggt
     1621 gccgttgctg atcgcccccg ccatgaaccg cgaaatgtgg gcgcatccag cgacgcagcg
     1681 caatctggcc cagctcaagg ctgacggcac cacagttttt gacgtgggaa ccggcgatca
     1741 ggcctgtggc gaaaccggtg acgggcgcat gctggagccc gaggagctgc tgcaggacct
     1801 ggtgagcttc tttctgccca aggttcttgc agggcagcat gttttggtca gtgccggacc
     1861 gacctatgaa gcgattgacc cggtacgcgg catcaccaat ctgtcgagcg gaaaaatggg
     1921 ctttgccatt gcccgtgcag cccacgaagc cggtgcacgc gtcacgctgg ttgctggccc
     1981 ggtcagtttg cctaccccac gtggtgtgga acgggtcaat gtgcgttcag cacaaaatat
     2041 gcttgaagcc tcggcggaat atgcgcagga cgctactgta tttatagcaa cggctgcggt
     2101 ggcggactgg cgtcctgcgc aggcttcgga ccagaagatc aagaaagacg gctctggcgc
     2161 cacacctcaa ctggattttg tggaaaaccc ggatatcctg gccacggtgg cgaaaactcc
     2221 ccgggctgct gctggtgcgt tgtactgtgt gggcttcgcc gcagaaagcc acgatttgtt
     2281 ggtccatgcc acggccaagc gcgcgcgcaa gggtgttccc ctgctggtgg gcaatattgg
     2341 tccagccacg ttcgggcagg acgacaatgc cctgctgctg gtggatgccc acggcgcaca
     2401 ggaaatccct cgcaactcca aactggtgct ggcccggcat ctcgtgaccg agatcgcgcg
     2461 gcgcctgcgc gctacacaac cctgataccc atgaaaatcg acgtcaaaat tctcgatcag
     2521 cgtctcgctg acaaccttcc tacgtatgcc acaccaggca gtgccggtct ggatctgcgt
     2581 gcctgcctgg atgcaccgct gaccctggcg cccaacgcgt ggcagctggt gcccaccgga
     2641 atggccatct acctgcacga cccggcttat gccgctctga tcctgccgcg ctcggggttg
     2701 ggtcacaaac acggcatcgt gctgggcaat ctggtcgggt tgattgacag cgactaccag
     2761 gggcagctga tggtgagcgc ctggaatcgc agttccaccg aattcacgat cgaacccatg
     2821 gagcgcattg cgcaactggt catcgtcccg gtggtacagg cgcagttcaa tatcgtcagc
     2881 gagtttccgg cgtcccagcg cggcgaaggc ggctacggct ctaccggaaa agcctagtgc
     2941 aacgtatcgc tgccggggcc gctttcgtgg aacggctgga tgcggaagaa tcccgacccg
     3001 gcggaaccgc gactgatttc ctcttcggtg gcttcccgga ctgactccac cttgatcgtg
     3061 atgcggatgg caatgcccgc cagcgggtgg tttccatcca gcaccacatg ttctgggtag
     3121 atgtcggtca cggtgtacag cgtgtctttg ggcgcatccg ggttgcagcc cgcaggcagg
     3181 gcagagccct ccatggtcaa cccttcttcc aattccgcag ggaacaatcc ccgcggctcc
     3241 aggaaaatca actggtcgtt gaaatcgcca aatgcctgtt cgggctcaat ctgcaactga
     3301 accgttgcgc cagccccatg cccctgcaag gcttcttcga tggctggcag caaatcctgc
     3361 ccgctgatca gaaattcgat gggttcgtcc agaacgtcca gtatctcgcc caaagtatct
     3421 ttcagcgtcc aggtaagggc aacgacgcat tgttgagtga tttccatagt gagagaattg
     3481 tcccatgaac atccaagaac cattgcaact gctcggcggt ttgtctcccc aggtcttcat
     3541 gcaacgccat tgggagaaga agccattgct gattcgccag gccattcctg gcttccagcc
     3601 gatcctcgac cgcacggaat tgctcgatat ggcggccatg gacgacgtgg aatcccgtct
     3661 ggtggtgcag gcggtgcctg gcgggcagaa agcctggaag ttccgccatg gcccgtttcc
     3721 ccgcaaatca ctgccgccat tcaaacaacg ggcgtggacg gtgctggtgc aggggatgga
     3781 cctgcacgac gagcgggtcc atgccctgat gaaccagttc cggtttgtac cggacgcgcg
     3841 cctggacgac ctgatgatca gctacgccac tgatggtggt ggcgtaggcc cgcactttga
     3901 cagctacgac gtatttttgc tgcaggccca tgggcgccgg cgctggcgca tcgggcggca
     3961 aaaggacatg agtctgctgc cggacatgcc cctcaaaatt ctcgccaact tcgagccgga
     4021 gatggagttt gtgctggagc ctggggacat gctgtatctg ccaccgcgtt gggcgcatga
     4081 cggtattgcc gaaggcgagt gcatgaccta ttccatcggc tttcgctccc ccaatatggg
     4141 tgaagtggcg cgggaagtgc tgcagcgcat ggcggagcag gctgatg
//
LOCUS       DAOSJM010000211         4167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18841_length_4167_cov_4.062743, whole genome shotgun sequence.
ACCESSION   DAOSJM010000211 DAOSJM010000000
VERSION     DAOSJM010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18841_length_4167_cov_4.062743"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKX82_08270"
     CDS             complement(<1..643)
                     /locus_tag="PKX82_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HOF52290.1"
                     /translation="MSEPLRIVVIAPDLIVNDQQDTHALEQAERSRSLRIGLLENGFN
                     LVASLPADVYLTERLAQLAPDMIIVDAESEARDALEHVVMATRDARRPIVMFTNDPDT
                     SNARAAVAAGVSAYIVAGLQPERIRPILDVALARFQQEQALRKELENTRSELQELSTE
                     LKDRKVIDRAKALLMERQNLSENDAYTRLRKAAMDKNLKLVEVAQRMLDVADLL"
     gene            complement(674..1420)
                     /locus_tag="PKX82_08275"
     CDS             complement(674..1420)
                     /locus_tag="PKX82_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF52291.1"
                     /translation="MLNDHDIADLERATLDAVAPHAVEALPGWLLPFDVSTIGRATSA
                     VPLSHALQDADIVHTIADRYATRGLTPAFRVADVPALANIHRELRRMGYRPEQPTQVQ
                     TGSARHMLQVLAPGQAVLTTQPTSAWSDVYLAPGFDPVDGAHRVQALSRSTCVVYASL
                     ADAQGPAAAGTASFGHGWAGIHGMRTALRCRGRGLARQILASLAQEAIARGFDRVFLQ
                     VDEGSTAALALYQRVGFVSAWRYHYWRMPR"
     gene            1446..2519
                     /gene="tsaD"
                     /locus_tag="PKX82_08280"
     CDS             1446..2519
                     /gene="tsaD"
                     /locus_tag="PKX82_08280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOF52292.1"
                     /translation="MVQNMLVLGIESSCDETGVALVRTRAQGVPELLSHALYSQIEMH
                     QAFGGVVPELASRDHIRRVIPLAQEVLGTAGCTLAEVDVVAYTRGPGLAGALLVGAGV
                     ACALGAALDKPVLGVHHLEGHLLSPFLSEDPPEFPFVALLVSGGHTQLMRVDGVGHYE
                     ILGETIDDAAGEAFDKSAKLMGLPYPGGPHLAKLALEGNPAAYALPRPLLHSGNLDFS
                     FAGLKTAVLVQSQRMVAAAGVARFEDLPREKQADLAASTQAAIVEVLLKKSLAALKET
                     GLQRLVVAGGVGANRDLRAQLDAACAKRKVRVHYPELHLCTDNGAMIAMAAAMRLQSG
                     SQQASRTYAFDVKPRWPLDAIAQ"
     gene            complement(2540..2917)
                     /locus_tag="PKX82_08285"
     CDS             complement(2540..2917)
                     /locus_tag="PKX82_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOF52293.1"
                     /translation="MFFAATPAHLRRNVYANAGRSLERYLDDALQGARQPQTSYTQDE
                     TSFTLSLDMPGIAKDQLSIAIEGNIVRISSKEGAQRNYRTAYELPQELDTALSEAKLE
                     NGVLTLKLAKKVPVKNVTELTVH"
     gene            3010..>4167
                     /locus_tag="PKX82_08290"
     CDS             3010..>4167
                     /locus_tag="PKX82_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOF52294.1"
                     /translation="MYKFACRVLKTASLALCAATFSASTVLAQGAGPGIKLAMVEALS
                     GPNANAGEAVFRNLLWAVERVNARGGVKVASGTLPLVLERYDSKGQVDEALSALRSAI
                     DNGARFVLQGNHSGIAAALIDTINKHNEREPNKRVIFLNYSAVDPILTNEKCSFWHFR
                     FDAHADMRMAALMDVIKDDKALKSVYLIGQDYSFGQAVLREAKRQLGAQRPDVQIVAE
                     ELHPMARVKDFLPYAAKIKASGAQAVITGNWGNDLTLLVKAARESGFDGKFYTFYGNA
                     LGAPAALGDAGVGRVVAVADWLPNVQTAGSEAFYKSFRVRYPLPVDDYVHMRMQLMVE
                     ALAQGLERVGKQPGAVTANAIDMVAVASQLEKAQVTMGGQSGSMRAGDHQFQ"
BASE COUNT          705 a         1175 c         1452 g          835 t
ORIGIN      
        1 cgagcaggtc cgccacgtcc agcatgcgct gggccacttc taccagtttg aggtttttgt
       61 ccatggcggc tttgcgcagc cgggtgtagg cgtcgttttc gctgaggttc tggcgctcca
      121 tcagcagggc cttggcgcgg tcgatgacct tgcggtcctt gagctcggtg ctgagttcct
      181 gcagctcgct gcgggtgttt tccagttcct tgcgcaaggc ctgttcctgc tggaagcggg
      241 ccagggccac gtccagaatg gggcggatgc gttcgggctg caggccggcc acgatgtagg
      301 ccgacacgcc ggctgccacg gccgcgcggg cattgctggt gtctgggtcg ttggtgaaca
      361 tgacgatggg gcgccgcgcg tcgcgggtgg ccatgaccac atgctccagc gcatcgcggg
      421 cttcgctttc ggcgtccaca atgatcatgt cgggcgccaa ctgggccaga cgttcggtga
      481 ggtacacatc ggcgggcagc gaggccacca ggttgaatcc gttttcgagc aggccaatgc
      541 gcaggctgcg tgagcgttcg gcctgctcca gcgcgtgcgt gtcctgctgg tcgttgacga
      601 tcaggtccgg ggcgatcaca acaatgcgca ggggttcaga catacatggg ttgacgcaat
      661 tttcgcgcca accctagcgt ggcatgcgcc agtagtggta gcgccatgcg ctgacaaagc
      721 cgacacgctg gtacagggcc agtgctgcgg tgctgccttc atctacctgc aggaatacgc
      781 ggtcgaaccc gcgcgcgatg gcctcctggg ccaggctggc aagaatctgc cttgccagac
      841 cacgcccacg gcagcgcagg gcggtgcgca tgccgtgaat acccgcccag ccatgaccga
      901 acgaggccgt gccagcggcc gccggacctt gcgcgtccgc cagactggca tagaccacac
      961 aggtgctgcg gctcagggcc tggacacggt gggcaccgtc taccgggtca aatccagggg
     1021 ccaggtagac gtctgaccac gcggaggtgg gttgcgtggt caaaacggcc tgtcccggcg
     1081 ccagcacttg cagcatgtgc cgtgccgaac ccgtttgcac ctgcgtgggt tgctcgggcc
     1141 ggtagcccat gcgccggagt tcacggtgga tgttggccag ggccggcacg tcggccacac
     1201 gaaaggccgg ggtgaggccg cgtgtggcat accggtcggc aatggtgtgc acgatgtccg
     1261 cgtcctgcag cgcatggctc agcggcaccg cgctggtggc ccggccaatg gtggagacgt
     1321 caaatggcag cagccatccg ggcaatgcct cgacggcatg gggcgctacg gcgtccaggg
     1381 tggcacgttc cagatcggcg atgtcgtggt cgttcagcat ggctgtactc taaactctgc
     1441 gccctatggt gcaaaacatg ctggtgttgg gaattgagtc gtcgtgcgat gaaaccgggg
     1501 tcgcgctggt gcgcacgcgt gcgcagggcg tcccggaatt gctgtcgcac gcgttgtaca
     1561 gccagatcga gatgcaccag gcatttggcg gcgtagtgcc cgagctggcc agccgcgacc
     1621 atattcgccg ggtgattccg ctggcgcagg aggtcctggg tactgcgggg tgcactctgg
     1681 ccgaggtgga tgtggtggcc tacacccgtg gtccggggtt ggcgggggcc ttgctggtgg
     1741 gggcgggtgt ggcctgtgcg ctgggcgcgg ctttggacaa gccggtgctg ggcgtgcacc
     1801 acctggaggg ccatctgctg tcgccgtttc tcagcgagga cccacctgaa tttccgtttg
     1861 tggcgttgct ggtgtcgggt ggacacacgc agctgatgcg ggtagatggg gtagggcact
     1921 acgaaattct gggtgaaacg attgacgacg ccgcgggtga ggcgtttgac aaatccgcca
     1981 agctcatggg cctgccgtac ccgggtggtc cccatctggc caagctggcg ctggaaggca
     2041 acccggccgc ctatgcactg ccgcgtccgc tgctacacag tggcaatctg gatttttcgt
     2101 ttgcagggct gaagaccgcg gtgctggtgc agtcgcagcg catggtggcc gcggcggggg
     2161 tggcgcggtt tgaagacctg ccgcgtgaga agcaggccga tctggctgca tccacgcagg
     2221 cggcgattgt ggaagtgctg ttgaagaagt cgctggccgc gctcaaggaa accggcctgc
     2281 aacggctggt ggttgccggt ggcgtggggg ccaaccgcga tctgcgtgcc cagctggatg
     2341 ctgcgtgcgc caaacgcaag gtgcgggtgc attacccgga gttgcatctg tgcaccgaca
     2401 acggcgcgat gattgccatg gcggcagcga tgcgcctgca aagtggttcg cagcaggcca
     2461 gcaggacgta cgcttttgac gtcaagccac gctggcctct ggatgcgatt gcgcaatagg
     2521 gtcagcgcag taacgcgact taatggacgg tgagttcggt gacgttcttc accggaactt
     2581 tcttcgccag cttcagggtc agtacaccgt tttccagctt ggcttcgctc agcgcagtgt
     2641 ccagttcctg gggcagttcg taggccgtgc ggtagttgcg ctgtgcgcct tccttgctgc
     2701 tgatgcgcac gatgttgccc tcgatggcaa tgctcaattg gtccttggca atgccgggca
     2761 tgtccagtga cagggtgaat gaggtctcgt cctgcgtgta gctggtttgt ggctggcgcg
     2821 cgccctgcag tgcgtcgtcc agatagcgct ccagcgagcg gcctgcgttg gcgtaaacat
     2881 tgcgacgaag atgggcgggt gtggcagcaa aaaacatggt gaactcctag tacactgcgc
     2941 ggcgctcacc gtgagcgtcg catgcctcca agctgcgacc gcttccttcc atttcaagag
     3001 aaattctcca tgtataaatt tgcttgccgg gtcttgaaaa cggcttcact ggcgttatgt
     3061 gctgccactt tttctgcgtc gacggtgctg gcgcagggtg ctgggcccgg tatcaagctg
     3121 gccatggtcg aagccctcag tgggcccaat gccaatgcgg gggaggcagt gtttcgcaac
     3181 ctgctgtggg cggtggagcg tgtgaatgcg aggggagggg tcaaggtcgc ttccggtact
     3241 ttgccgttgg tgctggagcg ctatgacagc aagggccagg ttgatgaggc actgtcggca
     3301 ctgcgctcgg ccatcgacaa cggtgcgcgt tttgtgttgc aaggcaatca ctccggtatt
     3361 gcggcagcct tgatcgacac catcaacaag cacaacgaac gcgagcccaa caagcgcgtg
     3421 atcttcctga actattctgc ggtcgatccc attctgacca acgagaaatg cagtttttgg
     3481 cacttccggt ttgacgccca cgcggatatg cgcatggcgg ccctgatgga tgtgatcaag
     3541 gacgacaagg ctttgaagag cgtctacctc attggccagg actacagctt tggccaggcg
     3601 gtcttgcgcg aggccaagcg gcagttgggc gcgcagcgtc ccgatgtaca gatcgtcgcg
     3661 gaagagttgc acccgatggc gcgggtgaaa gactttctgc cttatgcggc aaaaatcaag
     3721 gccagtggcg cgcaggcggt catcaccggc aactggggca atgacctgac gctgctggtg
     3781 aaggctgcgc gtgagagcgg gttcgatggc aagttctaca ccttctacgg caacgcactg
     3841 ggtgcaccgg ctgcgctggg tgatgctggc gtgggccggg tggtggcagt agccgactgg
     3901 ctccccaatg tgcagacggc gggtagcgaa gcgttttaca aatcgttccg tgtgcgctat
     3961 cccttgccag tcgatgacta tgtgcatatg cgcatgcagt tgatggtgga ggcgctggct
     4021 cagggtctgg aacgcgtggg caagcagcca ggcgctgtta cggccaacgc gatcgacatg
     4081 gtggcggtgg ccagccagct ggaaaaggcg caagtcacga tgggtgggca aagcggctcc
     4141 atgcgggctg gagaccacca gttccag
//
LOCUS       DAOSJM010000212         4162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18878_length_4162_cov_3.959094, whole genome shotgun sequence.
ACCESSION   DAOSJM010000212 DAOSJM010000000
VERSION     DAOSJM010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18878_length_4162_cov_3.959094"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PKX82_08295"
     CDS             <1..926
                     /locus_tag="PKX82_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF52295.1"
                     /translation="IKSRLFQWAQTVGWRRFCRSQKLPVSGGVPAWLDALVWPVLHTL
                     VAKPLLAQFGGRLRAAISGGAALSKPIAQCFLGLGLPILQGYGMTETSPVVAANGLDD
                     NDPTTIGRVLPGVEVRIGENQELQVRGPSVMRGYWKREADTAAAFVDGWLRTGDQAGI
                     ENGRIRILGRIKEIIVTSTGEKIAPADLELAITSDPLFEQAYTFGDNRPFIGSIVVLG
                     RAGWERMCAELGLPHQAPESLQAPAARQVALQRIRELTHGFSHYAQPKGVVLSLEPWT
                     PDNTLLTPTLKLKRNNLSARFAQDIEGLYRR"
     gene            1098..1817
                     /locus_tag="PKX82_08300"
     CDS             1098..1817
                     /locus_tag="PKX82_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOF52296.1"
                     /translation="MSATGLSVVVVDDKVVGGIYPEILQGIQRKDTCTFSIKAVPRAR
                     LEYLFESGAADVLIPASKTPERDAVGVFVPLIYNRATLVSLDTVRKPITTAQELIDSP
                     DLKVVLVRGFAYGAAYDDLVRELSRQGRVQYEVDPLSVARVLKNGSAHVTIMAPSILA
                     GAVQGDDRVRDMADKLRFEPIRELPWGDSGAYVSRTSLGEQDQKELVALLEHAARSGA
                     VWRGFQKYYSPKVLKESIRPR"
     gene            complement(1838..2506)
                     /gene="ureG"
                     /locus_tag="PKX82_08305"
     CDS             complement(1838..2506)
                     /gene="ureG"
                     /locus_tag="PKX82_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HOF52297.1"
                     /translation="MSTTPSPLHHIPHRTKNLPPLRVGIGGPVGSGKTTLLEMLCKAM
                     RDQYDLVAITNDIYTKEDQRLLTVSGALPADRIMGVETGGCPHTAIREDCSINLEAID
                     RMLVDHPDADIVFVESGGDNLAATFSPELSDLTIYVIDVAAGEKIPRKGGPGITKSDL
                     FVINKTDLAPYVGANLAVMEQDTIRMRTTPKGLKPFVMTNLKDLTGLREVIAFIETKG
                     MLKA"
     gene            complement(2566..3243)
                     /locus_tag="PKX82_08310"
     CDS             complement(2566..3243)
                     /locus_tag="PKX82_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HOF52298.1"
                     /translation="MLDASLMQLMWLASPALPIGGFSYSECLEAAVESERAATESDAS
                     AWLVDQLHLNLARADLPAVAQAIPAWRDGNTERIAQLNAWVLQTRESSELRAQTEQMG
                     KSLLDWLRNHTTATPAQIALLAGQEPTYPMAFALAASATQATVRECLLAYAFGWAENM
                     TQAAIKSVPLGQSAGQRILSALTAEIPAAIDHALGVTDDTRQAFSPMLAILSSQHEVQ
                     YSRLFRS"
     gene            complement(3264..3812)
                     /gene="ureE"
                     /locus_tag="PKX82_08315"
     CDS             complement(3264..3812)
                     /gene="ureE"
                     /locus_tag="PKX82_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HOF52299.1"
                     /translation="MLQVSKLLAQGADLSPAILKRSPSMELDWDVRQKSRFDAFDSLG
                     RQIGVFLPRGTVVRGGDVLVAADGSLINVVAAPQSVLKITHCSNHGSLYDLIRAAYHL
                     GNRHVPIELKPDHLKIEPDHVLADMLRAMHLIVNAVDEPFEPENGAYATGGHHGGHSH
                     GHDHAHGDDHGHVHGPGCGHKH"
     gene            complement(3824..>4162)
                     /gene="ureC"
                     /locus_tag="PKX82_08320"
     CDS             complement(3824..>4162)
                     /gene="ureC"
                     /locus_tag="PKX82_08320"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656514.1"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HOF52300.1"
                     /translation="AAMGDPNASIPTPQPVHYRPMFGAFGGALAKGSFTFVSQAGLNA
                     GIKDRFGLTKNVSAVKNIRAVRKQHMVHNSYLPVMEIDAQTYTVRADGQLLTCEPAVK
                     LPLAQRYFLF"
BASE COUNT          729 a         1215 c         1389 g          829 t
ORIGIN      
        1 ccatcaagtc gcgcttgttc cagtgggcgc aaacggtggg ctggcggcgg ttttgccgca
       61 gccagaagct gccagtgtct ggtggagttc ccgcctggct ggatgcgttg gtctggccgg
      121 tcttgcacac actggtggcc aagccgctgc tggcccagtt tggcgggcgc ctgcgcgccg
      181 cgatcagcgg cggcgctgca ctgtccaaac cgattgccca atgctttctg ggcctgggcc
      241 tgccgatcct gcagggctat ggcatgacgg aaacctcacc cgtggtcgca gccaatgggc
      301 tggacgacaa cgaccccacc accattggcc gcgtgctgcc aggtgtggag gtgcgcattg
      361 gcgagaacca ggagctgcag gtgcgcgggc ccagcgtgat gcggggttac tggaagcgtg
      421 aggccgatac ggcggcggcg tttgtggacg gctggttgcg caccggcgac caggcgggca
      481 tcgagaacgg gcgtatccgc atcctgggcc gcatcaagga aatcatcgtc acctccaccg
      541 gcgaaaagat tgcccccgcc gacctggagc tggccatcac cagcgaccct ttgttcgagc
      601 aggcctatac gtttggcgac aaccggcctt tcatcggcag catcgtggtg ctgggccgcg
      661 ccggctggga gcgcatgtgc gcagaactgg gcctgccaca tcaggcgcct gaaagcctgc
      721 aggcgcccgc tgcgcggcag gtggcactgc agcgcatccg cgagctgacc catgggtttt
      781 cgcactatgc gcagcccaaa ggggtggtac tgagtctgga gccgtggacc cctgacaaca
      841 cgctgctgac accgacgctc aagctcaaac gcaacaatct gtcggcccgc ttcgcgcagg
      901 acattgaggg gttgtaccga cgctaacaga gctttttggc agggcaaatc cggagagaca
      961 tggatacctc catgggttag catggttcca gtatgaagtc tgtacgattc agtagaaccc
     1021 ttcggtcact tgcgctggtc gtcgcggcgt tacccacaac tgccttggct ctttgttcgc
     1081 gacccatcaa cacacccatg tcggctaccg gcctgagtgt cgtggtggtg gatgacaagg
     1141 tggtgggcgg gatttatccg gaaattcttc aaggaatcca gcgaaaagac acttgcactt
     1201 tctccatcaa agcagtacca cgcgcccgac tggagtacct gtttgaaagt ggggctgccg
     1261 atgtcttgat ccccgccagc aagacgcccg agcgcgatgc ggtaggtgtt tttgttccgc
     1321 tgatttacaa ccgtgcaacg ctggtctcgc tggacactgt ccgcaaaccc atcaccaccg
     1381 cacaggaatt gatcgacagt cctgatctca aggtggtctt ggtgcgggga ttcgcgtacg
     1441 gggctgcata tgacgatctg gtgcgcgagt tgagccgcca ggggcgggtg cagtacgagg
     1501 tggacccgct ttccgtcgcc agggtgctga agaatggtag cgcccacgtc accatcatgg
     1561 cgccgtcgat tctggcgggg gccgttcagg gcgatgaccg tgtgcgggat atggcagaca
     1621 agctgcgctt tgaaccgatt cgcgaattgc cctgggggga cagcggtgcc tacgtctctc
     1681 gcacgtcact gggtgagcag gaccagaaag agcttgttgc cctgctggag catgcagcgc
     1741 gctctggggc cgtctggagg ggattccaga aatactattc cccgaaggtt ctcaaggaaa
     1801 gtattcgtcc gaggtagtgg ccggggcggt tggcggctca ggctttcagc atgcctttgg
     1861 tttcgatgaa ggcaatcacc tcccgcaacc cggtcaggtc tttcaggttg gtcatcacaa
     1921 acggcttcaa tcctttgggc gtggtgcgca tgcggatggt gtcctgctcc atcaccgcca
     1981 gattggcgcc cacatagggg gccaggtcgg tcttgttgat cacgaagaga tcgctcttgg
     2041 tgatgccggg accgcccttg cgcggaatct tctcgcccgc agccacgtcg atcacataga
     2101 tcgtcaggtc cgagagttcg gggctgaagg tggcggccag gttgtcgcca ccgctttcca
     2161 caaacacgat gtccgcgtcg gggtggtcca ccagcatgcg gtcgatggct tctagattga
     2221 tggagcagtc ttcgcggatg gcggtgtgcg ggcagccgcc ggtttccacc cccatgatgc
     2281 ggtcggccgg caacgcgccg cttaccgtta gcaggcgctg gtcttccttg gtgtagatgt
     2341 cgttggtgat ggcgaccagg tcgtactggt cgcgcatggc cttgcacagc atttccagca
     2401 gcgtagtctt gccggagccc acagggccgc cgatgcccac gcgcaaaggg ggcaggttct
     2461 tggtgcggtg ggggatatgg tgcaggggtg agggtgtggt ggacatggta atttgctatg
     2521 aaaagaagag ctgtttgcgc aatgaatacg agggctggag gccaattacg atctgaaaag
     2581 tcgtgaatac tgcacttcat gctggctgct caggatggcc agcatgggcg aaaacgcctg
     2641 gcgcgtgtcg tcggtcacgc ccagcgcgtg gtcgatggct gcgggaatct cggcggtcag
     2701 cgcggacagg atgcgctggc ccgcactctg gcccaatggc acggatttga tggcggcttg
     2761 cgtcatgttt tcggcccagc caaaggcgta ggccagcagg cattcgcgca ccgtggcctg
     2821 tgtggcgctt gcggccagcg caaaagccat ggggtaagtg ggctcctggc ccgccagcag
     2881 cgcaatctgc gccggcgtgg cggtggtgtg gttgcgcagc cagtccagca ggctcttgcc
     2941 catctgttcg gtttgtgcgc gcagttcgct gctctcgcgg gtttgcagca cccaggcgtt
     3001 gagctgggca atgcgttcgg tgttgccgtc gcgccaggcg ggtatggcct gcgccacggc
     3061 gggcaggtcc gcacgcgcca ggttcaggtg cagttggtcc accagccacg cagaggcatc
     3121 actctcggtg gcggcgcgct ccgattccac cgcggcttcc aggcattccg aataggaaaa
     3181 cccgccaatc ggtagcgccg gcgaggccag ccacatcagt tgcatcaggc tggcgtcgag
     3241 catggctttt tgcgccaacc gctttagtgc ttgtgcccac acccggggcc gtgcacatgc
     3301 ccgtggtcgt ccccgtgagc atggtcgtgt ccatgggaat ggccgccgtg gtgaccgccg
     3361 gtggcatagg caccgttctc gggttcgaag ggttcgtcca ctgcgttcac gatcaggtgc
     3421 atggcgcgca gcatgtcggc cagcacatgg tcgggctcga tcttcaggtg gtcgggcttg
     3481 agttctatcg gcacatggcg gttgcccagg tggtaggccg cgcggatcag gtcatacagt
     3541 gagccgtggt tgctgcagtg ggtgatcttc agcaccgatt gcggtgcagc caccacgttg
     3601 atcagcgagc catcggccgc caccagcaca tcgcccccgc gtaccacggt gccgcgcggc
     3661 aggaacacgc caatctggcg ccccaacgag tcgaacgcat cgaagcggct tttctggcgc
     3721 acatcccagt ccagctccat gctgggggag cgcttcagga tggcgggcga gagatcggcg
     3781 ccttgcgcca gcagtttgga gacttgcaac atggagaggt ggatcagaac aaaaagtagc
     3841 gttgggccag cggcaacttt accgcgggct cgcaggtcag caactggcca tcggcgcgca
     3901 cggtataggt ctgtgcgtcg atctccatga ccggcaggta gctgttgtgc accatgtgct
     3961 gcttgcgcac cgcgcggatg ttcttcaccg cgctcacatt cttggtcaga ccaaagcggt
     4021 ccttgatgcc ggcattcaat ccggcctgcg acacaaaagt gaatgagccc ttggccagtg
     4081 cgccgccaaa cgcgccaaac atcgggcggt agtgcacggg ctgcggtgtg gggatggagg
     4141 cattggggtc gcccatggcg gc
//
LOCUS       DAOSJM010000213         4149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_18958_length_4149_cov_5.227650, whole genome shotgun sequence.
ACCESSION   DAOSJM010000213 DAOSJM010000000
VERSION     DAOSJM010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18958_length_4149_cov_5.227650"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKX82_08325"
     CDS             <1..512
                     /locus_tag="PKX82_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOF52301.1"
                     /translation="KIELTFAKALRAILRQDPDIIMIGEIRDFETAQIAIQASLTGHL
                     VLATLHTNDAASAVTRLADMGIEPFLLSSSLLGVLAQRLVRKLCPECRGKGCPDCGMT
                     GYHGRTGVFELLVADEAIRGLIHRSAAEAEIRAAAIAGGMTLMREDGERLIQSGVTSR
                     EELVRVTRD"
     gene            657..974
                     /locus_tag="PKX82_08330"
     CDS             657..974
                     /locus_tag="PKX82_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HOF52302.1"
                     /translation="MIYKFKSQAAAEVIMLAANGDQMLTLVGKEPLPQGVITVAQIPD
                     AIAALEAAVATHAAAEFRQADHPHGELAATGDGVRLGARAAPFIELLRTSLQARNDVV
                     WGV"
     gene            1048..1347
                     /locus_tag="PKX82_08335"
     CDS             1048..1347
                     /locus_tag="PKX82_08335"
                     /inference="COORDINATES: protein motif:HMM:NF022160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HOF52303.1"
                     /translation="MKHYLLVLAMAASPLLANAADVKIDIGNVRIQAPGVTVTFGTRD
                     NRGYYWDGEQYRDPRYWKDHHGPRGEKYYTGRGNNGVPHQGGGFCPPGQAKKGNC"
     gene            1436..>1668
                     /locus_tag="PKX82_08340"
     CDS             1436..>1668
                     /locus_tag="PKX82_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52304.1"
                     /translation="MALQYQLKEGNYHLYDLSTPASKVTGEHRLRLMTDTVAIAFDAR
                     TGELREHGSPTRIHSWANNTRRRLRAEGALDRA"
     assembly_gap    1669..1768
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1778..2746)
                     /locus_tag="PKX82_08345"
     CDS             complement(1778..2746)
                     /locus_tag="PKX82_08345"
                     /inference="COORDINATES: protein motif:HMM:NF002334.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HOF52305.1"
                     /translation="MRLRPTSLGLGLVVAVACLVAWQRYAPQDATPTSAALPAAGSAQ
                     APFVRSMQDTLPDGNLQAILNGTVNGHFTPQVYAELRRLFDYYLSAVGEQSIPAITQQ
                     IRDELQRRLPPPQAQKAQRLLDLYIAFKRELVDLESKPALAGSGVQAIRNRQWAMQEL
                     RGRYFSQEESQGMFGFEDAYDTDAIARLEISQNPALNAAQKQQKLAALDAAMPSVLRN
                     ERDASSAVWRADQQAQEMRAKGASEDDIYRMRAKEFDAQAAARLAEVDREEADWKNRI
                     ARYREERTRLLAAQANASESERQSALAQLQQNLFSEAERPRLVAYE"
     gene            complement(2752..3615)
                     /locus_tag="PKX82_08350"
     CDS             complement(2752..3615)
                     /locus_tag="PKX82_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HOF52306.1"
                     /translation="MAAGYTQTTYPIVLVHGLFGFDNIGPVEYWYGIPSALRADGAKV
                     YTVQVSAANSTEVRGEQLLTQVKQILAATGATKVNLIGHSHGGPTTRYVASVRPDLVA
                     SVTSIGGVNKGSAVADLILGVAPPGSLTNAVVVSVTNGLATVINFLSGGGGLTQNSLA
                     AAQSLSTAGSAKFNAAHPYGVPTTACGEGAYTARGVSYFSWSGAKPYTNILDPIDPAM
                     ALTGLAFGGAKNDGLVASCSTHLGRVIRDDYALNHLDEVNQTVGLVNLFETNPVTLFR
                     QHANRLKNLGL"
     gene            3941..>4149
                     /locus_tag="PKX82_08355"
     CDS             3941..>4149
                     /locus_tag="PKX82_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspF"
                     /protein_id="HOF52307.1"
                     /translation="MPAYSFEALSANGETRKGIIDADSARAARGLLRAQALVPLAVEA
                     VMAGNAGADGASGTSSTTLWSKRVF"
BASE COUNT          744 a         1263 c         1296 g          746 t
ORIGIN      
        1 ccaagatcga actgaccttt gccaaggccc tgcgcgccat cctgcgccag gaccccgaca
       61 tcatcatgat cggcgagatc cgcgatttcg agaccgcgca gatcgccatt caagcctcgc
      121 tcactggcca tctggtgctg gccacgctgc acaccaacga cgctgccagc gcggtcaccc
      181 gcctggccga catgggcatt gaacctttcc tgctgagctc gtccctgctg ggcgtactgg
      241 cgcagcggct ggtgcgcaag ctgtgccccg agtgccgtgg caaaggctgc ccggactgcg
      301 gcatgaccgg ctaccacggc cgcaccgggg tgtttgagct attggtggcc gacgaagcca
      361 ttcgcggact gatccaccgt agcgcggccg aagccgagat tcgcgcagcg gccatcgccg
      421 gtggcatgac actgatgcgc gaggacggcg agcgcttgat ccagagtggc gtcacgtcgc
      481 gcgaagagct ggtgcgcgta acgcgggact gagctaccta cacacgacca tccccgagtg
      541 ggggcccatg gtgcgaaaag aagcctctgg tctgccggga tatgttggct gcaccacaga
      601 cagcagtgca ctgcaaggaa tatggtgcag ccttcagcaa cacgcggggt tccaccatga
      661 tttacaaatt caagtcccag gctgcggcag aggtcatcat gcttgcagcc aacggggacc
      721 agatgctgac ccttgtgggc aaggagccct tgccccaggg cgtcatcacc gtggcgcaga
      781 taccagacgc cattgcggca ctcgaagccg cagtggcaac ccatgcagca gccgagttcc
      841 gtcaggcgga ccatccgcat ggggagcttg ctgcaacagg cgacggtgtc agactgggtg
      901 cccgcgcggc tccgttcatt gaattgctgc gcaccagtct gcaggccagg aatgatgtgg
      961 tctggggggt gtgagtgaca tgccgcagat actccactgc gcaggcttca ctacaatccg
     1021 tccaggttat caacaaggac tgtccagatg aaacactacc tcctggttct cgccatggcg
     1081 gcgtcaccgc tgcttgccaa cgcggcggat gtaaaaatcg atatcggcaa tgtccgtatc
     1141 caggcaccgg gcgtcaccgt cacgttcggt acccgtgaca accggggcta ctactgggac
     1201 ggcgagcagt accgcgaccc ccggtactgg aaggaccacc acggtccgcg gggcgagaag
     1261 tactacaccg gccgcggcaa caacggcgtt ccgcaccagg gcggcggctt ctgtcctccg
     1321 gggcaggcca agaagggcaa ctgctgaaag tattcgaatg gccggttttc cggccttttt
     1381 ttcgctttag ttttcctggc cgccgcccag aatcagtgcc atcgaggaga acacgatggc
     1441 actgcaatac caactcaaag aaggcaacta ccacctctac gacctgagca ctccggccag
     1501 caaggtcact ggcgaacacc gcctgcgact gatgaccgat acggtggcga ttgcctttga
     1561 tgcccgcacc ggcgagttgc gcgagcatgg cagccccacc cgcatccact cctgggccaa
     1621 caacacccgc cgccgcctgc gcgccgaagg tgcattggac cgtgccaann nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnngg cccgcagcta ttcgtaagcc accagacgcg
     1801 gacgttctgc ttcgctgaac aggttttgct ggagctgcgc cagcgccgac tggcgctccg
     1861 actcggacgc attggcctgg gcggcgagta gtctggtgcg ctcttcgcgg taacgcgcga
     1921 tgcggttttt ccagtccgct tcctcgcggt cgacctcggc cagccgggcc gcggcctgtg
     1981 cgtcaaattc tttggcacgc atgcggtaga tatcgtcttc gctggcgcct ttggcgcgca
     2041 tctcctgggc ctgctggtcg gcacgccaca cggcactgct cgcgtcgcgt tcgttgcgca
     2101 gaacggaggg catggcggcg tccagtgcgg ccagcttctg ttgcttctgg gctgcgttca
     2161 aagccgggtt ctggctgatt tcgaggcgcg caatcgcgtc ggtgtcgtag gcatcttcaa
     2221 aaccgaacat gccctgcgat tcttcctggc tgaaatagcg tccgcgcagc tcctgcatgg
     2281 cccattggcg gttgcggatg gcctgcacgc cgctgcctgc cagtgcgggc ttgctttcca
     2341 ggtccaccag ctcgcgcttg aaagcgatat acaggtccag cagacgctgt gccttctgcg
     2401 cctggggggg cggcaggcgc cgctgtaatt catcgcggat ctgctgcgtg atggctggaa
     2461 tactttgctc gcccacggcg ctcaggtagt agtcaaacag ccggcgcagc tctgcgtaga
     2521 cctgcggggt gaaatgtccg ttcacggtgc cattcaggat ggcttgcaga ttgccgtcgg
     2581 gcagggtgtc ctgcatggag cggacaaagg gagcctgcgc gctgcctgca gcgggcagtg
     2641 cagcgctggt gggggtggca tcctggggcg catagcgttg ccatgcaacc aggcacgcca
     2701 ccgcaaccac cagccccaga cccagggatg tggggcgcag gcgcatgggg ctcacagacc
     2761 caggtttttc agacggttgg cgtgctggcg gaacagcgtc accgggttgg tttcaaacag
     2821 gttgaccagg ccgaccgtct ggttcacttc gtcgaggtgg ttcagtgcgt agtcgtctcg
     2881 gatcacacgg cccaggtggg tcgagcagct ggccaccaga ccgtcgttct tggcgccacc
     2941 aaaggccagt ccggtcaggg ccatggcggg gtcaatggga tccagaatgt tggtgtacgg
     3001 tttggcgcca ctccacgaga agtaagacac gccacgcgcg gtataggcgc cttcaccgca
     3061 agccgtagtg ggcacgccgt aggggtgcgc cgcattgaac ttggcggagc cggccgtgct
     3121 cagggactgt gcagcggcca gcgaattctg ggtcaggcca ccaccaccgg agaggaagtt
     3181 gatgacggtc gccagaccgt tggtcaccga aaccaccacc gcattggtca gtgatccggg
     3241 aggtgccacg cccagaatca ggtctgccac ggccgagccc ttgttcacgc cgccaatgga
     3301 ggtgaccgac gccaccagat cggggcgcac cgaggccacg tagcgggtgg tggggccgcc
     3361 atggctatgg ccaatcaggt tgaccttggt ggcgccggtg gccgccagga tctgcttgac
     3421 ctgggtcagc agctgctcgc cacggacttc ggtgctattg gcagccgaga cctgtacggt
     3481 gtacaccttg gcaccgtccg cgcgcagggc actcggaata ccgtaccagt attccaccgg
     3541 cccgatgttg tcaaagccaa acaggccatg caccagcacg atggggtagg tggtctgggt
     3601 ataacccgcc gccatacccg atgccgggag caatgacagg gccagcaggc aggcccccaa
     3661 ccagttcttc agaatctttt tcacaatgtc tcctagtgga gcctcaaggc tctcaaatgt
     3721 tgaaatgaat cggtctgaga aggcagtgcg acctcgttgg taggggcgtt gtcgctgggt
     3781 gacgcggtgc cggtctgttg tgtgaaccgt tcccgtccaa aaaagcacgg tcgtgcgttt
     3841 tgatgcatgg taggcgcttg tgtggtgttt gtcatggtgg aaaacccgag atgccgcgcc
     3901 gattcagggg gctggtcctt tcacccgcta atatcgcccc atgcccgcgt attccttcga
     3961 agccctgagc gccaacggcg aaacccgcaa gggaatcatc gacgccgaca gcgcccgtgc
     4021 cgcccgcggg ctgttgcggg cccaggcact ggtgcccctg gccgtggaag cggtcatggc
     4081 cggtaatgcg ggcgctgacg gcgcatccgg caccagcagc accaccctgt ggagcaaacg
     4141 cgtgttcag
//
LOCUS       DAOSJM010000214         4124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19101_length_4124_cov_4.318752, whole genome shotgun sequence.
ACCESSION   DAOSJM010000214 DAOSJM010000000
VERSION     DAOSJM010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19101_length_4124_cov_4.318752"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PKX82_08360"
     CDS             <1..906
                     /locus_tag="PKX82_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOF52308.1"
                     /translation="VVVQALLLTHNLDISSARQIATRGSLSGALIGGLVFGAGMIMTR
                     GCASRLLVLSANGNLRALLSGLIFAVAAQASLSGALSPLRATISAWWTVDAGPSRDLL
                     ALTGLGPWGGLVAGLVWMAAAMYFSWRSPKHSAWVWLGAVGTGLMVAAAWWFSYFLST
                     QSFEPIQVQGLTFSGPSAEWLMRVLASPTPRIGFEFGLLPGVFAGSFLGALIGRDLKL
                     EGFKDGYSMTRYIAGAILMGFGSMLAGGCAVGAGMTGGSIFALTAWITLIGMWAGAGL
                     MDKLLDSQPLEQQIFDSPPVAAKAP"
     gene            complement(924..1544)
                     /locus_tag="PKX82_08365"
     CDS             complement(924..1544)
                     /locus_tag="PKX82_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOF52309.1"
                     /translation="MTTGVEMKQVGKWAGAAVAVLFCSSVWAQADEARAKKIVSGVCS
                     LCHGMEGESSSEIFPKLAGQHWEYVAKQLDNFKSGKRESTAMSQMVAKLTPDEMVALG
                     KYFEKKKSAPVPSNEPSLAAVGQYIYTSGNKFSGVPACAGCHGPNAQGTSSLPRLAGQ
                     YATYTESQLNQFNRRQRTNDNAVMHTIVSKMTPLEMAAVAEYLSSK"
     gene            complement(1544..2827)
                     /locus_tag="PKX82_08370"
     CDS             complement(1544..2827)
                     /locus_tag="PKX82_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOF52310.1"
                     /translation="MKRRQFVQGVGAAGSLGALGLVTGCATTGGANGPKVVVVGGGYG
                     GATAAKYVRMWSDYGIDVTLVEPNSSFISCPISNLVLGGSKTMADITTPYDNLSRRHG
                     VRLVQDMVTRIDPEKRVVMLAGGGELPYDRLILSPGVDFMWENLPGMAKAGAKDKVLH
                     SWKAGAQTVALRKQLEAMPDGGVYALSIPLAPYRCPPGPYERACQVAHYFSKAKPRSK
                     VLILDANDDVTSKGPLFKKAWKERYEGIVEYRPKHKVVDVDAATNTLKFEFNDDVKAS
                     VLNVIPDMRAGDIAVKSGLATANKRWCEVDFLTFESVAVKNVHVLGDSIQIAPAMPKS
                     GHMANQHGKTCAAAVVALLTGKQPNSLPIYNNTCYSFVSDEDVVHVASVHRYDAEKKT
                     MLTVPGSGGVSAAANELEGRYALAWARNIWADTLA"
     gene            complement(2843..3151)
                     /locus_tag="PKX82_08375"
     CDS             complement(2843..3151)
                     /locus_tag="PKX82_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I cytochrome c"
                     /protein_id="HOF52311.1"
                     /translation="MKWNLTTLAVALASVCATTQAQNLDPLQVRSWAAACANCHGTNG
                     VAQSGNESLAGGNKDELLKKMLDFKTGKKPATIMHQLSKGYSDEQLAALAGYFAAQKK
                     "
     gene            complement(3300..3644)
                     /locus_tag="PKX82_08380"
     CDS             complement(3300..3644)
                     /locus_tag="PKX82_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOF52312.1"
                     /translation="MGKQSSPNVAGAQSDGDQIFQLAAELFRVLSAPMRLRIIKSLCE
                     HEKNVSELLSEIDTTQPNMSQHLNTLYVSGVLAKRREGVQIYYRISDVRVAELCRVVC
                     KQVAVDAELSPA"
     gene            complement(3781..3855)
                     /locus_tag="PKX82_08385"
     tRNA            complement(3781..3855)
                     /locus_tag="PKX82_08385"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3821..3823),aa:Val,seq:cac)
     gene            complement(3882..>4124)
                     /locus_tag="PKX82_08390"
     CDS             complement(3882..>4124)
                     /locus_tag="PKX82_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HOF52313.1"
                     /translation="AELLADVYINMTRGQDALLMEAAPVEEKGVVSVQVDLSRIDLPV
                     LEANAQELGLHEEVLGQIDKSSGGKTVWKKSLENAI"
BASE COUNT          788 a         1223 c         1163 g          950 t
ORIGIN      
        1 gtcgtggtgc aggcactgct actcacccac aacctggaca tcagttcagc gcgccagatt
       61 gccacccgtg gaagcttgtc gggtgcactg attggcggcc tggtttttgg cgccggaatg
      121 atcatgaccc gggggtgcgc cagccgcctg ctggtgctgt cggccaatgg caacctgcgc
      181 gccctgctct ctggactcat ttttgctgtg gccgcgcaag cctcgctgtc gggcgctttg
      241 tccccgctgc gtgccaccat cagcgcatgg tggacggtgg atgccggacc gtcgcgcgat
      301 cttctggccc tcaccggcct gggaccgtgg ggaggactgg ttgccgggct ggtatggatg
      361 gctgcagcca tgtatttctc ctggcgcagc cccaaacaca gcgcttgggt ctggctgggt
      421 gccgtaggca ccggtctgat ggtggcagca gcctggtggt tcagctactt tctgtccacg
      481 caatcgtttg agcccatcca ggtacagggc ttgacgttca gcgggccatc cgcggaatgg
      541 ctgatgcggg tactggcttc gccgacaccc aggatcggtt tcgagttcgg actgctaccc
      601 ggcgtgtttg cgggttcgtt tttgggtgct ctgatcggtc gggatctgaa actcgaaggt
      661 ttcaaggacg gttacagcat gacacgctac attgctggcg ccatactgat gggctttggc
      721 tccatgctgg cgggcggctg tgcggtgggt gcggggatga ccggcggatc gatctttgcg
      781 ctgaccgcct ggatcacgct gattggaatg tgggccggtg ccggcctgat ggacaaattg
      841 ctcgacagcc agccactgga acagcagatt ttcgattccc ccccagtggc cgccaaggct
      901 ccgtgaagag cctctggcgt cacctacttg ctgctgaggt attcggcgac agccgccatt
      961 tccagagggg tcatcttgga aacgatggtg tgcatgaccg cattgtcatt ggtacgctgg
     1021 cgcctgttga actggttcaa ctggctctcc gtataggtag cgtactgtcc tgccaacctg
     1081 ggcagactgc tggtaccctg cgcattgggg ccatggcaac ccgcgcaggc cggcacaccg
     1141 ctgaacttgt ttccgctggt gtaaatgtat tgccccacag cagccagact gggttcattg
     1201 gatggtacag gtgctgactt cttcttctcg aaatacttgc ccagagcgac catctcgtcg
     1261 ggcgtcaact tggcgaccat ctgggacatg gcggtgctct cccgcttgcc cgatttgaag
     1321 ttatcgagtt gcttggccac gtactcccaa tgctggccag ccagcttggg aaatatctcg
     1381 ctcgacgact caccctccat gccgtggcac agagagcaca cgccggagac aattttcttt
     1441 gcgcgggcct catccgcttg cgcccatacc gaactgcaga acaggacggc caccgccgcc
     1501 ccagcccatt tacccacttg cttcatctcg actcctgtcg tcatcaggcc agggtatctg
     1561 cccaaatatt gcgtgcccat gccagcgcat aacggccttc aagttcgttg gcagcagccg
     1621 aaacaccacc cgagcccggc actgtcagca tggtcttttt ctccgcgtca tagcgatgca
     1681 cgctggcgac atggaccacg tcttcgtcac tgacaaagct gtagcaagtg ttgttgtaaa
     1741 tgggcaggct gttgggttgc ttgccggtaa gcagcgccac gacggcggcc gcacaagtct
     1801 tgccatgctg gttggccatg tgcccggatt tgggcatggc gggggcgatc tggatggaat
     1861 cccccagcac atgtacattc ttgacggcca cggactcgaa cgtcaggaag tccacctcac
     1921 accaccgctt gttggctgtt gccagcccgg acttgacggc aatatctccg gcacgcatgt
     1981 caggaatgac gttgagaacg gatgccttca cgtcgtcgtt gaattcaaac ttcagggtgt
     2041 tggtagcggc atccacatcc accaccttgt gcttgggacg gtattcaacg ataccttcgt
     2101 aacgctcttt ccaggccttt ttgaacaaag gtcccttgga ggtgacatca tcgttggcgt
     2161 caagaatcag taccttgctc ctgggtttgg ctttgctgaa gtagtgggcc acctggcagg
     2221 cacgctcgta cggcccaggg gggcagcggt acggagccag cgggatcgag agcgcatata
     2281 cgccaccatc aggcatggct tcgagttgct tacgcagtgc cacggtctgg gcgccagctt
     2341 tccagctgtg cagcaccttg tctttggcac cggccttggc catgccaggc aagttttccc
     2401 acatgaagtc cacacccgga gaaagaatca ggcggtcata gggcaattcg ccgccgccag
     2461 ccagcatgac cacccgcttt tcaggatcga tccgggtcac catgtcctgc accagacgca
     2521 caccatggcg tcggctcagg ttgtcatagg gtgtggtgat atccgccatg gtcttgctgc
     2581 cacccagcac cagattggag atggggcagg aaatgaaact gctgttgggc tcgaccaggg
     2641 tcacatcaat gccgtaatcc gaccacatgc gcacgtattt ggcagctgtt gcaccgccat
     2701 agccaccacc caccaccacg accttggggc catttgcgcc accggtcgtt gcacagcccg
     2761 ttaccagtcc cagcgcaccc agggagcctg cagcgcccac accttgaaca aattgacgac
     2821 gtttcatcat gatcgtgcct ccttattttt tctgtgctgc aaagtaaccg gccagcgctg
     2881 ccagttgctc gtcggaatag cccttggaga gctggtgcat gatggttgcc ggctttttgc
     2941 ctgtcttgaa atccagcatc ttcttgagca gctcatcctt gttgccaccg gccagggact
     3001 cgttcccgga ttgcgccacg ccattggttc catggcaatt ggcacaagct gcagcccagc
     3061 ttcgcacctg caaggggtcc aggttttgtg cctgcgttgt ggcacacaca gatgccagcg
     3121 caacggccag cgtggtgagg ttccatttca ttgtcggctc tcctaggttt gaaatcatcc
     3181 aacaagacgg gatggaatca atccatcact tccgtcttat atttagtgtc aaatgtatgc
     3241 cgttccccgg agctcgctgt agggtgtaaa ccctgacggg tgttgtcaac cccgtgtaat
     3301 caagctggtg atagctcagc gtctacggcg acctgtttgc acacgacgcg acagagttcc
     3361 gccacccgaa catcggaaat gcgatagtag atttgcacac cttcgcgacg cttggcaagg
     3421 acaccagaaa catacaatgt attgaggtgc tgggacatgt tgggctgggt ggtgtcgatt
     3481 tcagacagca gctcactcac atttttctcg tgctcgcaaa ggctcttgat gatgcgaagc
     3541 cgcataggag cggacagcac gcggaacaac tcagctgcga gttgaaaaat ttgatcgcca
     3601 tcgctctgcg cgcctgccac gttcggtgat gactgcttgc ccattgtcga gttccccttg
     3661 cctgcatatg cccgagcatt gtaatgttga ccctggaatt gcgtgcaatc ccgcggtgtt
     3721 ctggggacac aaaaaaagga cctggtgcaa aacaccaggt cctttttgat accaataaat
     3781 tggtgggcag tgcaagtttc gaacttgcga cccctgcagt gtgaatgcag tgctctaccc
     3841 ctgagctaac cgccctgaat tctgttcagg aaagcctgtg attatatagc attttccagc
     3901 gacttcttcc ataccgtttt accgccactg gatttatcga tctggcccag cacttcctca
     3961 tgcagcccca gctcttgcgc attggcctcc agcaccggca ggtcgatgcg cgacagatcc
     4021 acctgaacgg acacgacccc cttctcctcg accggagcgg cttccatcag tagtgcatcc
     4081 tgtccgcgcg tcatgttgat atagacgtca gccagcaact cggc
//
LOCUS       DAOSJM010000215         4122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19111_length_4122_cov_4.228424, whole genome shotgun sequence.
ACCESSION   DAOSJM010000215 DAOSJM010000000
VERSION     DAOSJM010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19111_length_4122_cov_4.228424"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="PKX82_08395"
     CDS             <1..785
                     /locus_tag="PKX82_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52314.1"
                     /translation="RRMHQAAGLMGLNQGRFPRLTAFVSHGDPQSELPLLWKLCSAIV
                     DMGHSVTVLDGTTPETGENPGLEQVLDFTAWREVEPDAHAWNILPSMHGLHALTTFSN
                     PATRCLSELGRAFAHDGILIMYSNAQRMSTLLASSGVRPLLSISPAKTSLLTSYLALK
                     RLLIKGHLEPAVANVVQYQDSGLETSETVANNLRDCAKYFLDFDVNILPIVTPSGEDR
                     PGMDVQRVALSMIENAIPLDTGWTTPSLRADVPAATRAARSH"
     gene            785..1501
                     /locus_tag="PKX82_08400"
     CDS             785..1501
                     /locus_tag="PKX82_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HOF52315.1"
                     /translation="MYTAKGQLDRNALIKQYQPLVRRLAHHMMAKLPPSVEVDDLIQV
                     GLIGLSEALTRYEASQGVQFETFATQRIRGAMLDELRENDWMSRGSRKSQKDIEAAMR
                     RLEHKLGRSPAESEIAAELGMSLPDYQSLLGKVRGTQLVYLEDMTGRSEDDDTFLDRH
                     VADTDADPLNMLRDQRLRESLVTAIKTLPEREQYIMSMYYEQDMNLKEIAAVLDVTES
                     RVCQLHSQSIARLRAKMRAH"
     gene            complement(1511..1870)
                     /locus_tag="PKX82_08405"
     CDS             complement(1511..1870)
                     /locus_tag="PKX82_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52316.1"
                     /translation="MTHLDLVEQQFNEVSVVLAEVNPIQLEMASTAMQRLASDLVELL
                     RTAQGDEAMRLTVVQRLQALSQGTMLLRSQLHRQAALVEQALKVVMPGGPVSTYADGG
                     PYGSAVRQSGAFKVLAA"
     gene            complement(1928..2254)
                     /gene="flgM"
                     /locus_tag="PKX82_08410"
     CDS             complement(1928..2254)
                     /gene="flgM"
                     /locus_tag="PKX82_08410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOF52317.1"
                     /translation="MKIPAPSDPSSSTSVAKSTNAPKPGQGAPATASSTAAQSTRSAG
                     VAVTVSTLARALEKPEATQASDIDTVKVATVKAAIQDGTYVVNPEAIADKLLSNAQEM
                     LNRTTG"
     gene            complement(2349..3086)
                     /gene="flgA"
                     /locus_tag="PKX82_08415"
     CDS             complement(2349..3086)
                     /gene="flgA"
                     /locus_tag="PKX82_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOF52318.1"
                     /translation="MPSTLVRIQNPIRQTGSRWLLAVAFLFAGWAQAQQADYPSMAQD
                     WARKAAVAALPPGGQALRLDVSVGPLDSRLKLAPCGNVEAYLPPGSRLWGRSRVGLRC
                     VDGMARWNVSLPATIKAFGPAWVIKSQVMAGVAMGAQDVVEAEVDWAEEPQAVLREPG
                     SWVGQMATRQLGTGQTLRQGMVKAPQVFQAGAQVRVVAQGAGFQVTGEAQALSAGVVG
                     QMARVRMDSGRVATGIVLDVRTVKIEL"
     gene            3280..3717
                     /gene="flgB"
                     /locus_tag="PKX82_08420"
     CDS             3280..3717
                     /gene="flgB"
                     /locus_tag="PKX82_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOF52319.1"
                     /translation="MFANLTNALDFQAKALVIRADRQRAIASNIANADTPGYAGRDVN
                     FKEAMNAALGGNSLTLSRSAPTGGTSEGATNPRHIPLESTTGTLGRDNLDYTTQSQPA
                     MDGNSVDMDRERANFVDNSVRYEATLRFINGSSRTILSAIQGQ"
     gene            3721..>4122
                     /gene="flgC"
                     /locus_tag="PKX82_08425"
     CDS             3721..>4122
                     /gene="flgC"
                     /locus_tag="PKX82_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOF52320.1"
                     /translation="MSMFSIFNVSGSAVSAQSQRLNVVASNLANADAVAGPDGQAYKS
                     RQVVFETVRLGSDASSGVKVSAIKESNEPFKRVHNPTHPSADAEGYVNQSNVNPVEEM
                     VNMISASRSYQNNVEVMNTAKTLLLKTLQMGQ"
BASE COUNT          856 a         1367 c         1156 g          743 t
ORIGIN      
        1 atcgacgtat gcaccaggct gccggcctca tggggctcaa ccagggacgg ttcccgcggt
       61 tgaccgcatt cgtgagccac ggggacccgc agtccgaact cccgctgctg tggaagctgt
      121 gttctgccat agtggatatg ggccacagtg tcaccgtgct ggacggaacc actccggaaa
      181 ccggtgagaa ccccggactg gaacaggttc tcgatttcac tgcatggcgc gaagtcgaac
      241 cggatgcgca tgcctggaat atcctgcctt cgatgcacgg actccatgca ctcaccacct
      301 tttccaatcc agccacccgt tgcctgagcg agctgggacg tgcgtttgcg cacgacggca
      361 tcctcatcat gtacagcaat gcacagcgca tgtcgaccct gttggcgtcc agcggcgtgc
      421 ggccgctgct gtccatctct ccggccaaga cctccctgct caccagctat ctggccctca
      481 aacgcctgct gatcaagggc cacctggaac cggcggtggc gaatgtggtt caataccaag
      541 acagcggact tgaaacgtcc gagactgtgg ccaataatct gcgtgactgc gccaagtatt
      601 ttctggattt cgacgtgaac atcttgccca tcgtgacccc atccggcgaa gaccgccccg
      661 ggatggatgt gcagcgcgtg gccctgtcca tgatcgaaaa tgccattccc ctggacaccg
      721 gatggaccac gccatccttg cgtgccgatg tgccagctgc aacccgggcc gccaggagcc
      781 actgatgtat accgccaaag gccagttaga tcgcaatgcg ttgatcaagc agtaccagcc
      841 actggtgcgg cggctggcgc accacatgat ggccaagctg cccccgagcg ttgaagtgga
      901 tgatctgatc caggtcggac tgatcggact gtcagaagca ttgacccggt acgaagcctc
      961 ccagggagtg cagtttgaaa cgtttgccac gcagcgcatc cgcggcgcca tgctcgacga
     1021 gctgcgggaa aacgactgga tgtcccgcgg gtcccgcaag agccagaagg acattgaagc
     1081 cgccatgcgc cggctggagc acaagctggg ccgcagcccg gcagagtcag aaatagcggc
     1141 cgagctgggc atgagcctgc ctgattacca gtcactcctg ggaaaagtcc ggggcacaca
     1201 actggtctat ctggaagaca tgaccgggcg cagcgaggac gacgacacct tcctggaccg
     1261 ccatgtggcc gacacggacg cagacccgtt gaacatgctg cgcgaccagc ggctgcgcga
     1321 atccctggtc acagccatca agaccctgcc cgaacgcgag cagtacatca tgagcatgta
     1381 ctacgagcag gacatgaacc tcaaggaaat tgcagccgtt ctcgatgtga ccgagtcccg
     1441 cgtctgccaa ctccacagcc agtccatcgc ccgcctgcgc gccaagatgc gcgcgcattg
     1501 aatccgggta tcaggctgcc agcaccttga aggcgccgct ctggcgcacg gccgaaccgt
     1561 aaggcccacc atcggcatag gtggataccg gaccgccggg catgaccacc ttgagtgcct
     1621 gctccaccag ggccgcttgg cggtgcaact gggagcgcaa cagcatggtg ccttgggaca
     1681 aggcttgcag gcgctgcacg actgtcagcc gcatggcctc gtcaccctgc gcggtacgca
     1741 gcaactccac caggtccgag gccagacgct gcattgccgt actggccatt tccagctgga
     1801 tcggattgac ctccgccaaa acgacagaaa cctcgttgaa ctgctgctca acgaggtcaa
     1861 gatgtgtcaa gaatgaattt tgtgtcattt cagggccttt gcacatgcaa gcatgcgcag
     1921 gccccactca acccgtagtg cggttgagca tttcctgcgc gttggaaaga agtttgtccg
     1981 caatcgcttc cgggttgacc acataggtgc catcctggat agccgccttc accgtggcga
     2041 ccttcaccgt atcgatgtcc gacgcctgtg tagcttcggg tttctccagc gcacgcgcca
     2101 gagtggacac tgtgaccgcc acaccggccg accgtgtgct ttgcgctgcc gtagaactgg
     2161 cggtcgcggg agcaccttgc cctggcttgg gcgcattggt gctcttggcg accgaagtcg
     2221 aactggacgg gtctgatggt gccggaattt tcatcgtgtt ctccaaaact acgatacgcg
     2281 tagcctcgta cgattgctat cggcctgaat ctaccggact ttaggctttt ttgcattgca
     2341 gcagccattt aaagttctat tttgactgtg cggacatcca gtacgatacc ggtcgcgacg
     2401 cgtccgctgt ccattcgcac gcgcgccatc tgccccacca caccggccga cagcgcctgc
     2461 gcctcgcccg tcacctggaa tcctgcgccc tgcgccacga cgcgcacctg ggctccggcc
     2521 tgaaatacct gcggtgcctt gaccatgccc tgccgcagtg tctggccggt tcccagctgg
     2581 cgggtcgcca tctggcccac ccagcttccg ggctcccgca ataccgcctg gggctcttcg
     2641 gcccaatcca cctcggcctc gaccacatcc tgagccccca tggcaacgcc agccatcacc
     2701 tgggacttga tcacccaagc aggaccaaac gccttgatgg ttgcgggcag ggacacattc
     2761 cagcgtgcca tgccgtccac acaccgcaga ccgacccgac tgcggcccca caggcgcgag
     2821 cctggcggca aataggcttc cacattgccg cagggcgcga gcttgaggcg actgtccagt
     2881 ggccccacac tcacatccag gcgcaaagcc tgcccgccgg gtggcaaagc agccaccgca
     2941 gccttgcgcg cccaatcctg tgccatcgac gggtaatccg cctgctgggc ctgcgcccag
     3001 cctgcaaaca ggaatgcaac cgccagcagc cagcggctac cggtctgtcg gatggggttc
     3061 tggatacgca caagcgttga aggcatcagt gttctccggg ccaagggccc tgtctgttgc
     3121 caagactata ggtaacgccg gtcgcaccaa acccctcaaa tggcagccat tccccccact
     3181 attcgacggc taaaaaatta aaggttttcc cataatcgtt ccacgtcaca tggttggggc
     3241 tgcacctgac cacctgacca actattcaga ggtgctccca tgtttgccaa tttgaccaat
     3301 gcgctggact ttcaagccaa ggccctggta atccgcgcag accggcagcg cgcgattgcc
     3361 agcaacattg ccaatgccga cacgccaggc tacgccggcc gcgacgtcaa cttcaaggag
     3421 gcgatgaacg ccgccctggg aggcaattcg ctgaccctct cccgttctgc acccactggc
     3481 ggcaccagcg aaggcgccac caacccgcgc cacattccgc tggaaagcac caccggaacc
     3541 ctgggccggg acaacctcga ctacaccacc cagagccagc ccgccatgga tggaaatagc
     3601 gtggatatgg accgggagcg cgccaacttt gtcgacaact cggtgcgcta cgaagccact
     3661 ctccgattca ttaacggcag ttcccgcacc atcttgagtg ctattcaggg gcaataaacc
     3721 atgtccatgt tttccatctt caacgtctcc ggcagcgccg tcagtgcgca gtcgcagcgt
     3781 ctcaatgtgg tggccagcaa cctggccaac gccgatgcgg tggccggacc cgacggccag
     3841 gcctacaaga gccggcaggt ggttttcgaa acggtacggc tggggtccga cgcttcctcg
     3901 ggcgtcaagg tcagcgcgat caaggaaagc aacgagccct tcaagcgcgt gcacaacccc
     3961 acccacccgt cggcagatgc cgaaggctat gtgaaccagt ccaacgtcaa tccggtggag
     4021 gagatggtca acatgatttc cgcctcacgc tcctaccaga acaacgtgga agtcatgaac
     4081 accgccaaaa ccctgctgct caagacgctg caaatgggcc aa
//
LOCUS       DAOSJM010000216         4114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19155_length_4114_cov_4.096083, whole genome shotgun sequence.
ACCESSION   DAOSJM010000216 DAOSJM010000000
VERSION     DAOSJM010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19155_length_4114_cov_4.096083"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PKX82_08430"
     CDS             complement(<1..1302)
                     /locus_tag="PKX82_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOF52321.1"
                     /translation="MNPTAHPSNPTGTFEVLGACPHDCPDTCSMVTTVRDGIAIKVHG
                     NAAHPATHGALCTKVSRYPERTYHPERILKPLRRTGPKGSGQFETVEWDTALADIATR
                     LKAIAARDPQAILPYSYAGTMGQVQGESMATRFFNCLGASQLDYTICAAAGGEGLVQT
                     LGGKVGMRVQFFAESRLILIWGSNSIGSNLHFWRHVQTAKRNGAKVICIDPRRTETAE
                     KCHEHIALMPGTDAALALALMHELIQNDWLDHDYIARYTSGWEPLRARALEWPPERAA
                     ATCGISVEQIRSLARDYGMAGVRGEPAAIRMNYGLQRVHGGGNAVRAIASLPALTGAW
                     RHRAGGVLLSSSGTYAVNKAALQRPDLRTHRPARTINMVTIGDDLLRSASASFGPAIE
                     ALVVYNSNPVTVAPESSKVVAGFARQDLFTVVLEHFQTDTAD"
     gene            1340..2536
                     /locus_tag="PKX82_08435"
     CDS             1340..2536
                     /locus_tag="PKX82_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOF52322.1"
                     /translation="MNLIEAIVASAPGIAAIRRDLHAHPELCFKEVRTADVVATKLAE
                     WGVPVHRGLGTTGVVGIVKAGTSTRAIGLRADMDALPMQEFNTFAHASQHPGKMHACG
                     HDGHTAMLLAAAQYLANHRNFDGTVYLIFQPAEEGGGGAREMIRDGLFEKFPVEAVFG
                     MHNWPGSEVGHFAVSAGPVMASSNEFTITIRGKGGHAAMPHNAVDPVPVACQMVQAFQ
                     TIISRNTKPIEAGVISVTMIHAGEATNVIPDSCELQGTVRTFSIELLDTIERRMREVA
                     DHTAAAYGASCEFVFARNYPPTINSAREAEFARKVMVGIVGADHVTAQEPTMGAEDFS
                     YMLMAKPGCYSFIANGDGAHREMGHGGGPCMLHNPSYDFNDTLIPLGATYWVRLAESW
                     LGGGAA"
     gene            2533..3141
                     /locus_tag="PKX82_08440"
     CDS             2533..3141
                     /locus_tag="PKX82_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52323.1"
                     /translation="MTTWIDLTPRDRSQELPAAIEREVSPGQYRYVFEHEGEQVNDPS
                     HDVTGQLFVSPRKYGFSIRGFQGGKTAWARDFANGTMLLSSPDGQSHVLSPRFPARIV
                     FLGADGAILQDSGGERRVARESSEFVTVRLTVSATVDLNGESQELARVQIMRMLDVAL
                     AQRMLPAGSDIRLVDHTIEQATVQPDRRTPSSDTDFSQLLDL"
     gene            complement(3149..3916)
                     /locus_tag="PKX82_08445"
     CDS             complement(3149..3916)
                     /locus_tag="PKX82_08445"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOF52324.1"
                     /translation="MTFLALDIGNTRLKWAQYDAPRPDAKLLHHGAEFLEHIDRLAEG
                     PWSNMQTPTHVLGCVVAGDAIKRRVQDQMEWWDVAPQWVVASESEAGLRNGYDHPARL
                     GADRWVAMIGAYHRMLGQGPAKPMVVVMVGTAVTVEAVDASGKFLGGFILPGHGIMLR
                     ALESGTAGLHVPTGEVREFPTNTSDALTSGGTYAIAGAVECMVQHVRAHCGAEPKCIM
                     TGGAGWKMAPSMTRPFELVDNLIFDGLLQMAKDRFPA"
     gene            complement(3923..>4114)
                     /locus_tag="PKX82_08450"
     CDS             complement(3923..>4114)
                     /locus_tag="PKX82_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52325.1"
                     /translation="PDGGKLVLLCRSGVRSIAAAKRATELGLEAYNILEGFEGDPDAH
                     AHRGNKGGWRMRGLPWRQG"
BASE COUNT          755 a         1247 c         1317 g          795 t
ORIGIN      
        1 gtctgccgtg tccgtctgga aatgctccag cacgacggtg aacagatcct ggcgcgcaaa
       61 gccagccacg actttggacg attcaggcgc cacagtgacc gggttgctgt tgtacaccac
      121 cagtgcctca atggcgggcc caaaggatgc actggcagac cgcagcagat cgtcgccaat
      181 ggtgaccatg ttgatggtgc gtgccgggcg gtgggtgcgc aggtcaggcc tttgcagggc
      241 ggccttgttg accgcatagg ttccagagct ggacagcaat acaccgccag cccggtgccg
      301 ccacgcccct gtcaacgccg gcaggctggc gatggcgcgt accgcattgc cgccgccatg
      361 cacccgttgc agaccgtagt tcatgcgaat ggccgcaggc tcaccccgaa caccggccat
      421 gccgtagtcc ctggccagcg aacggatctg ttccacagaa atgccgcaag tggctgcggc
      481 acgctctggc ggccactcca atgcccgtgc gcgtagcggt tcccagcccg aggtgtagcg
      541 ggcaatgtag tcgtggtcca gccagtcgtt ctggatcaat tcgtgcatga gggccaaagc
      601 cagcgcagca tcggtacccg gcatcagggc gatgtgttca tggcattttt cggcggtctc
      661 ggtgcgcctg gggtcgatgc agatgacctt ggcaccattg cgtttggccg tctggacatg
      721 gcgccagaaa tgcaggttgc tgccaatcga gttgctgccc cagatcagga tcagtctgga
      781 ttcggcgaaa aactgcaccc gcatacccac cttgccgccc agcgtctgaa ccagtccttc
      841 gccccccgct gcggcacaaa tggtgtagtc cagttgcgat gcccccaggc agttgaaaaa
      901 ccgggtcgcc atgctctcgc cctgtacctg tcccatggtc ccggcatagc tgtagggaag
      961 gatggcctgg ggatcgcgcg cggcgatggc cttcagccgt gtggcaatgt cggccagcgc
     1021 ggtgtcccac tcaaccgttt caaactgccc tgaccccttg gggccagtgc gtcgcagggg
     1081 tttcagaatc cgttccgggt ggtaggtgcg ctccgggtag cgcgacacct tggtacacag
     1141 cgcgccgtgg gtggccggat gggctgcatt gccatgcacc ttgatggcta tgccgtcgcg
     1201 tacggtggtc accatggagc aggtgtccgg gcagtcatgg gggcaggcgc ccagtacttc
     1261 gaaagtgcca gtagggttgg atgggtgcgc ggttggattc atgctggcaa tggtaaaccc
     1321 gcagcgccat aatgtccgga tgaacctgat tgaagcgatt gttgccagtg cgcccggtat
     1381 cgcggccatc cgccgtgatc tgcatgcgca tccggagttg tgtttcaagg aggtacgcac
     1441 cgcggatgtg gtggcgacca agctggcgga atggggcgtc ccggtgcacc gcgggctggg
     1501 caccacgggc gtagtcggca tcgtgaaagc gggcacatcc acccgcgcca ttggcttgcg
     1561 ggccgacatg gatgccttgc ccatgcagga attcaacacg ttcgcgcacg ccagccaaca
     1621 ccccggaaaa atgcatgcgt gtgggcacga tggacatacc gccatgctgc tcgcggcggc
     1681 gcagtacctt gccaaccacc ggaactttga cggtacggtg tacctgatct ttcagcctgc
     1741 ggaagaggga ggcggtggcg cccgcgaaat gatccgtgac ggtttgtttg aaaaatttcc
     1801 ggtcgaggcg gtatttggca tgcacaactg gccaggttcc gaggtcggcc atttcgcggt
     1861 gagtgctggg ccagtgatgg cgtcgagcaa cgagttcacc atcaccatcc gcggcaaggg
     1921 cggccatgcc gccatgccgc acaacgcggt cgacccggta ccggtggcgt gccagatggt
     1981 gcaggcgttt cagaccatca tcagccgcaa caccaaaccc atcgaggcgg gcgtcatttc
     2041 agtcaccatg atccacgcag gggaggcgac caacgtcatc cctgacagct gcgagttgca
     2101 gggcacggtg cgcacctttt ccattgaact gctggacacg attgagcggc gcatgcggga
     2161 agtggccgac catacagcgg ctgcttatgg cgcgagttgt gagtttgtct ttgcgcgcaa
     2221 ttacccgccc accatcaatt cggccaggga agcggaattt gcacgcaagg tcatggtggg
     2281 catcgtcggt gcagaccatg tgactgcgca ggagccgacc atgggcgccg aagatttttc
     2341 ctatatgttg atggccaagc ccggttgtta cagcttcatc gccaatggcg atggtgccca
     2401 ccgcgagatg ggccatggag gaggcccctg catgctgcac aaccccagct atgacttcaa
     2461 cgacacattg attcctctgg gcgctactta ttgggtgcgc ctggcggaat cctggctggg
     2521 tggaggagca gcatgacaac ctggattgat ttgacccccc gcgaccggag ccaggagctt
     2581 cccgcggcca ttgaacgcga agtatcaccg gggcagtacc gctatgtgtt tgagcacgaa
     2641 ggcgagcagg tcaatgaccc gtcccacgat gtgaccgggc agctgtttgt ctctccgcgg
     2701 aagtatggtt tttcgattcg cggcttccag ggcggcaaga ccgcctgggc acgggacttt
     2761 gccaacggca ctatgctgtt gtccagcccg gatggccagt cgcatgtgct gtcgccgcgg
     2821 tttcctgcgc gcatcgtctt ccttggagcg gacggagcga ttctgcagga ttcgggtggg
     2881 gagcgccgtg tggcgaggga atcatcagaa ttcgtgacag tgcgcctgac cgtgagcgcc
     2941 acggtcgatc tgaatggtga atcccaggag ttggcacgtg tccagatcat gcgcatgctg
     3001 gatgtggcat tggcgcagcg catgttgcct gccgggtccg acatacggct ggtggatcac
     3061 acgatcgagc aggcaacggt acaacccgat cggcgaacgc cgtccagcga caccgatttt
     3121 tcgcagctgc tggacctgta gcgctccttc aggcagggaa cctgtccttg gccatttgaa
     3181 gcagaccgtc gaaaatcagg ttgtcgacca gctcgaacgg acgggtcatg ctgggggcca
     3241 tcttccagcc ggcaccgcca gtcatgatgc atttgggttc tgcgccgcag tgcgcgcgaa
     3301 catgctgcac catgcactcc acagcaccgg caatggcgta ggtgccgcca ctggtcaatg
     3361 catcactggt attggtgggg aactcccgaa cctctccggt cggtacgtgc agaccggcgg
     3421 ttccagactc cagtgcgcgc agcatgatgc cgtggcccgg caggatgaag ccacccagga
     3481 atttgccaga ggcatccacg gcttccaccg tgactgcggt gcccaccatg acgacgacca
     3541 tcggcttggc cggaccttgc cccagcatgc ggtggtaggc gccaatcatg gcgacccagc
     3601 ggtctgcccc gagccgggcg gggtggtcat atccgttgcg caggccggcc tcggactcgg
     3661 atgccacgac ccattgcgga gccacatccc accactccat ctggtcttgc acacgccgtt
     3721 tgatggcatc gcctgcgact acacagccca gcacgtgcgt aggcgtctgc atattgctcc
     3781 aggggccttc ggccaggcgg tcaatgtgct ccaggaactc ggcgccatgg tgcaatagtt
     3841 tggcatcggg gcggggggcg tcgtactggg cccacttgag gcgcgtattg ccaatatcca
     3901 gtgcaagaaa agtcatgttg ttctagcctt ggcgccaggg gagtccacgc atccgccagc
     3961 cacccttgtt gccgcggtgt gcatgggcat cgggatcgcc ttcaaagcct tccaggatgt
     4021 tgtaggcctc caggcccagc tcggtcgccc gcttggctgc tgcgatcgag cgcacaccgc
     4081 tgcggcacag caggacaagc ttcccgccgt cggg
//
LOCUS       DAOSJM010000217         4076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19362_length_4076_cov_3.644864, whole genome shotgun sequence.
ACCESSION   DAOSJM010000217 DAOSJM010000000
VERSION     DAOSJM010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19362_length_4076_cov_3.644864"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..346
                     /locus_tag="PKX82_08455"
     CDS             161..346
                     /locus_tag="PKX82_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52326.1"
                     /translation="MNKLLAVLVATTFATMSFSALAGSHGGAMKEKEKAEACKKMDPK
                     TADEKMKAECKALAEKK"
     gene            complement(353..1093)
                     /gene="queC"
                     /locus_tag="PKX82_08460"
     CDS             complement(353..1093)
                     /gene="queC"
                     /locus_tag="PKX82_08460"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOF52327.1"
                     /translation="MHTTALVLFSGGQDSTTCLAHALSRYERVETVAFDYGQRHHVEL
                     DARLNVLREIQAQFPQWAPKLGEDHLLDLAVLGKVSETSLTRDTAFKMEASGLPNTFV
                     PGRNLLFLTLAAALAYRRGLQVLVTGVCETDYSGYPDCRDDTMKAMQLALSLGMDQRF
                     LIETPLMWIDKAATWELAWELGEKSGVPNGGQALVDLIVEHTHTCYLGDRVHRQVWGY
                     GCGTCPACELRANGFKKKRTRGPAVLEK"
     gene            complement(1153..1578)
                     /locus_tag="PKX82_08465"
     CDS             complement(1153..1578)
                     /locus_tag="PKX82_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52328.1"
                     /translation="MIPVAPVNPSVQAHPSIEPTPGVVNLVNPALQRQQPTEGEPVYT
                     SVADPLKNKVQEKAPHDWTIHRPTPEKVEDPPPKPLSQVLMDHLRTMWTASASAIQIE
                     QVKEQVTPAEPAVPTQVPGDLAREILTYQPTRIKKTENV"
     gene            complement(1860..3305)
                     /locus_tag="PKX82_08470"
     CDS             complement(1860..3305)
                     /locus_tag="PKX82_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOF52329.1"
                     /translation="MRKLIVPATFTALVLSMQGVCAQTPEQRSTLTDQREVAVTIYND
                     NLALVKDQRRIQLKTGATALAFRDVSARMRPETALLRSVSAPGALSVLEQNFDFDLLT
                     PQKLLEKYVGQTVNVVRTNPATGNETTESATVLSANNGVVLQIGQRIETGIPGRLVFG
                     SVPANLRDRPTLVMSLDNTAAAVQDVELSYLTAGLAWKADYVVELNAADDRLDMSGWV
                     TLTNTSGTSYRNARLQLVAGDVNQVAPELAQRGRAVLMAAPAMAKASSDVAQESLFEY
                     HLYTLGRPTTIAENQTKQVALLSASGVPARKELLLRGNDYYYQSSVGELGQKLKVAVY
                     VEFENKESARLGMPLPKGVIRVYKKDASGNAQFIGEDRIDHTPKGEKVRLKLGDAFDV
                     TADKKQTDFKKLGGTGKYNAVFESAYEMVLKNAKKEAVVVTVQEPMPGDWQILSASHP
                     HTKGASNTAVWSVPVPAEGRIVLTYRALVRY"
     gene            3397..3645
                     /locus_tag="PKX82_08475"
     CDS             3397..3645
                     /locus_tag="PKX82_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HOF52330.1"
                     /translation="MTDTTSRPPLPDHLCVDPRSPHHVAAVFEHDIGIRFNDRERMDV
                     EEYCISEGWVKVPAGKTVDRRGKPLLIKLKGKVEAFYL"
     gene            3657..>4076
                     /locus_tag="PKX82_08480"
     CDS             3657..>4076
                     /locus_tag="PKX82_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF52331.1"
                     /translation="MTARAGSALPKVLWPLLFGNFVIGTGVMVVPGTLNDISSSLDIS
                     VAVAGQLITAAAVVMCLGAPLFASIVAGWDRRRLLTLSLLWYAVFLGLSALMPSFGSL
                     LLVRVLAVVSPAIFTPQAAACVGLLVLPEQRGRAITFV"
BASE COUNT          750 a         1226 c         1206 g          894 t
ORIGIN      
        1 gcataggtgg aactggcggt tgccagcacc atgagcgcta ccaccaatgg ctcggcaatc
       61 tgtttgaata aacctgtcaa cgtaaacgcc ctcatgtttg ttgtacattg gctcgccacc
      121 tattccgttg gcagcatttc tttattattg gagttcacct atgaacaaac ttttggccgt
      181 cttggtagca accacattcg caaccatgtc attcagcgcg ctcgctggat cccatggtgg
      241 tgccatgaaa gagaaagaaa aagcggaagc atgcaaaaaa atggacccca aaaccgctga
      301 cgaaaaaatg aaagccgaat gcaaagcatt ggcagaaaaa aagtaagcta atttactttt
      361 ccagaacagc gggcccacgg gtccgctttt ttttgaatcc attggcccgc aattcacagg
      421 ccgggcaagt accgcagcca tatccccaaa cttggcgatg cacccggtcg cccaggtagc
      481 aggtatgggt gtgttccacg atcaggtcca ccagcgcctg tccgccgttg ggcacacccg
      541 acttttcgcc cagctcccac gccagctccc aggttgcagc cttgtcgatc cacatcagcg
      601 gagtttcgat caggaaacgc tggtccatcc ccagcgagag tgccagctgc atggccttca
      661 tggtgtcatc gcggcagtcg gggtagccgg agtagtctgt ttcgcacacc ccggtcacga
      721 gcacttggag accgcggcgg taggccagtg cggcggccag ggtcaggaac agcaggttac
      781 gcccgggcac aaaggtgttg ggcaggccac tggcctccat cttgaaagcc gtgtcgcgcg
      841 tcagcgaggt ttcactcacc ttgcccaaca ccgccagatc cagcagatgg tcttcgccca
      901 gcttgggcgc ccactgcgga aactgcgcct ggatttcgcg cagcacattc aaacgggcat
      961 ccagctccac atggtggcgt tgaccatagt caaaagcgac ggtttcaacg cgctcatagc
     1021 gcgacaaggc gtgcgcaagg caggtggtgg aatcctgtcc accggagaaa agtacaaggg
     1081 ctgtggtgtg catggccgct atggtagggc aaccctgcag aatggcgatc aagcgaacgc
     1141 cggtgaccgg actcagacgt tctcggtctt cttgattctg gttggctggt aagtcaggat
     1201 ttccctggcc agatcaccgg gaacctgggt tggcacagcg ggctcggccg gcgtcacctg
     1261 ttccttcacc tgttcgatct ggatcgcgct ggcgctggcc gtccacatgg tgcgcaaatg
     1321 gtccatcagc acttgcgaaa ggggcttggg cggtgggtcc tccacctttt ccggcgtagg
     1381 ccgatggatg gtccagtcgt gcggggcttt ttcctgcacc ttgttcttca gcggatcggc
     1441 cacgctggtg tacaccggct caccttcggt gggctgctgc cgttgcagcg ccgggttcac
     1501 caggttcacc acgcccggtg tgggctcgat ggacggatgc gcctggacgg acgggttgac
     1561 cggagcaacc gggatcaccc tgtttgcccc agacgaagcc aaatctgcgc cggcggggcg
     1621 cacattcggg gttcgatcaa tggatggcaa ctgcattttg gttaaggcgg tgaggcccct
     1681 ccggtgactt ttagatgctg acgtacgttt cttaacgaca gatttaggcc ggtatttagg
     1741 cctattttta aaaatttaga aaaatttttc gttcgccata ccaaattcac ctgaatttgc
     1801 accaatctgg tgcaaattct cttgcatcct cccccgccct gccagttacc cctgcttctt
     1861 caatagcgga ccagtgcgcg gtacgtcagg actatccggc cctcggcggg gaccggcaca
     1921 ctccagacgg ctgtgttcga ggcgcctttg gtatgggggt ggctggcaga caggatttgc
     1981 cagtcgccgg gcatcggctc ctgcacagtc actacaacgg cttctttctt ggcgttcttc
     2041 agcaccattt cgtaggcact ttcgaacacc gcgttgtatt tgccggtacc gcccagcttc
     2101 ttgaagtcgg tctgcttctt gtcggcggtc acatcaaagg catcacccag cttgaggcgt
     2161 accttctcgc ccttgggtgt gtgatctatc cggtcttcac caatgaactg ggcgttgcct
     2221 gacgcatcct ttttgtagac acggatgacg cccttgggca ggggcattcc gaggcgcgcg
     2281 ctttccttgt tctcaaactc tacatacacg gccaccttga gcttttgccc cagttcgccc
     2341 acgctgctct ggtagtagta atcgttgccg cgcaacagca gctccttgcg ggcaggcacg
     2401 ccgctggccg acagcagggc gacctgtttg gtctggttct cggcaatcgt agtgggacgg
     2461 cccaaggtgt acaggtggta ttcaaacagc gactcctgcg ccacgtcgct cgatgccttg
     2521 gccatggccg gtgcggccat cagcaccgca cgcccgcgct gcgccaactc gggtgccacc
     2581 tggttcacgt cgcccgccac cagttgcaac cgggcattgc ggtaactggt tccgctggtg
     2641 ttggtcagcg tgacccagcc cgacatgtcc agccggtcat cggctgcgtt gagctccacc
     2701 acatagtccg ccttccaggc cagtccagcg gtcaggtagc tgagctccac atcctgcacc
     2761 gcagcagccg tgttatccag ggacatcacc agggtgggcc gatcgcgcag gttggccggt
     2821 acgctgccaa acaccaggcg gccgggaata ccggtctcga tgcgctggcc gatttgcagc
     2881 accacgccgt tgttggccga cagcaccgtg gcggactcgg tggtctcatt gcccgtagcc
     2941 gggttggtac ggaccacgtt gaccgtctga cccacatatt tctccagcag cttctggggg
     3001 gtgagcaggt caaagtcaaa gttctgctcc agcacactca gcgcaccggg tgcgctgacg
     3061 ctgcgcaaca gcgcggtttc aggccgcatg cgggcactca catcccggaa tgccagcgcc
     3121 gtggcaccag tcttgagctg gatccggcgc tggtctttca cgagcgccag gttgtcgttg
     3181 tagatggtga cggccacttc gcgctggtct gtcagcgtgc tgcgctgctc cggggtttgg
     3241 gcgcagacac cctgcattga cagtacaagg gccgtgaagg tcgcgggaac aataagtttg
     3301 cgcatggcgt gggctttctg gaacggtgag ggtgcgatta ttgtgcgcca ccggtccatt
     3361 agacttgggg tctcgctttg ccgatacaaa taccccatga ccgataccac ttcccgcccc
     3421 ccacttcccg accacctctg tgtggatcca cgcagccccc accatgtggc ggccgtgttt
     3481 gagcacgata tcggcatccg cttcaatgac agggagcgca tggacgtcga ggaatactgc
     3541 atcagcgaag gctgggtcaa ggttccggca ggaaagacag tggaccgcag aggcaaaccg
     3601 ctgctgatca agctcaaggg taaggtcgaa gccttctacc tctaggtccc tctgccttga
     3661 ccgcccgcgc tggcagcgcg ctgcccaaag ttctctggcc cctgctcttt ggcaactttg
     3721 tcattggcac gggggtgatg gtggtgcccg gcaccctcaa tgacatcagc agttcgctgg
     3781 atatatcggt tgcggtggcc ggccagctga ttaccgccgc agcggtggtg atgtgtctgg
     3841 gcgctcccct gtttgccagc attgtggccg ggtgggaccg gcggcgcctg ttgacactgt
     3901 ccctgctgtg gtacgcggtt tttctgggtc tgtcggccct gatgcccagc tttggttcgc
     3961 tactgctggt gcgcgtgctg gccgtggttt caccagccat cttcacaccg caggcagcag
     4021 cgtgcgtggg gctgctggtg ttgccggaac aacgggggcg ggccatcacc tttgtc
//
LOCUS       DAOSJM010000218         4071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19398_length_4071_cov_3.892928, whole genome shotgun sequence.
ACCESSION   DAOSJM010000218 DAOSJM010000000
VERSION     DAOSJM010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19398_length_4071_cov_3.892928"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="PKX82_08485"
     CDS             complement(<1..1171)
                     /locus_tag="PKX82_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOF52332.1"
                     /translation="MATHSRSLFPGVTGTLTAATLGWVAGTALQLQQPDLGSPVVYMA
                     FMALALVGYAYTATKNIANLPGFLAAGLTVGALAFGATGLRSALFLQHALDPALEGRD
                     VMVVGVVADMPQSNEAGLRFRLEVESAQLDGVALQLPAKVDVAWYGGVYTTGSELVGL
                     QRQPLDVRAGERWSMTLRLKAPHGSQNPHGFDYELWLWEQGVGATGYVRAGVRDTPPQ
                     RITQTWKHPVALARQALRERIFASVGQRQSAGLIAALVVGDQRAIDRVDWDVFRATGV
                     AHLMSISGLHITMFAWGAALLVGWLWRRSGRLCLALPAPTAALCGGLLLATAYAVFSG
                     WGVPAQRTCTMLATVSLLRLTGARWPWPQVWLLACAVVVAVDPWALLQPGFWLSFV"
     gene            1251..>4071
                     /locus_tag="PKX82_08490"
     CDS             1251..>4071
                     /locus_tag="PKX82_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOF52333.1"
                     /translation="MSIHAALNHVTHYTYDRLVNLGPQVIRLRPAPHCRSKVISYSLK
                     VEPSTHFVNWQQDPFSNYQARLVFPEKTTEFKVTVDLVVEMAVYNPFDFFLEPEAEEF
                     PFSYEANLKQELAPYLIADPVTPMLQGFLDKIDRTKRRSVIFLVDLNTMVHKAVNYTI
                     RMEPGVQTPEETLSLASGSCRDSAWLMVQLLRNCGLAARFVSGYLIQLTPDVKSLDGP
                     SGTEVDFTDLHAWCEVYLPGAGWIGLDATSGLLAGEGHIPLACTPQPSGAAPIEGGVD
                     ESEVEFSHHMQVTRIYESPRVTKPYTPEQWADVMALGDQVDAELVAGDVRLTMGGEPT
                     FVAVNDRDAAEWNTDALGPTKRGFATALVQKLRDEYGQGGFLHFGQGKWYPGEQLPRW
                     ALNIYWRADKQPVWRNPALFTDERDPTHYTSEDAGYFARTLAAKLGLTDQYIQTAYED
                     TWYYLWRERRLPVNVDPFNSKLDDEMERARLRRVFEQKLDTPVGYVLPIKPAIAGGGP
                     RLAGPGWVTGPWFFRDERMYLMPGDSPMGLRLPLDSLPWVSKGDFPYLIEQDPYAPRD
                     ALPAHDAFARRYTPNYASPSINSSAAPTAPAGNGGGVDGMAYLAGAVPRREATRKLQS
                     ATGNAVNTVTANAQSTTQAEPANFAREPNRNESAHWITRTALCVEVRDPRRASGPKAE
                     AVGTKSGVMYVFMPPLERLEDYLDLLAAIEATAESLKMKIVLEGYPPPRDPRLKLLQV
                     TPDPGVIEVNIHPVTNWKELVYNTEFLYNAAFESRLSAEKFMTDGRHTGTGGGNHFVM
                     GGATPSDSPFLRKPELLASLLLYWHNHPSLSYLFSGMFVGPTSQAPRVDEARNDQLYE
                     LEIAIEQIYKNRELYGQSMPPWLVDRTLRNILIDVTGNTHRSEFSIDKMYSPDSSTGR
                     LGLLELRAFEMPPHPHMSSVQQLLLR"
BASE COUNT          803 a         1435 c         1178 g          655 t
ORIGIN      
        1 cgacgaaact cagccagaaa cccggctgca acaatgccca cgggtccacg gctaccacca
       61 cggcgcaggc cagcaaccag acttgcggcc agggccaacg cgcaccggtc aagcgcagca
      121 gtgagacggt ggccagcatg gtgcaggtgc gctgtgcggg cacgccccag ccggaaaaca
      181 cggcgtaggc cgtggccagc agcaggccac cgcagagcgc tgcggtcggt gccggcaatg
      241 cgagacacag gcggccggag cgtcgccaca accagcccac cagcaaggct gcaccccagg
      301 caaacatggt gatatggagc ccagagatac tcatcagatg ggccacaccg gtagcccgga
      361 acacatccca atccacccgg tcgatggcgc gctggtcgcc caccaccagc gcggcaatca
      421 gcccggcgga ttgccgctgc ccaacgctgg caaaaatacg ttcccgcaga gcctgccgtg
      481 ccagcgccac cgggtgtttc caggtttgtg tgatgcgctg cggtggcgtg tccctgaccc
      541 cggcccgcac atagccggtc gcccccacgc cctgctccca cagccacagt tcgtagtcaa
      601 acccatgcgg attctggcta ccgtgtggcg ctttcaggcg cagggtcatg gaccagcgct
      661 cgccggcgcg cacatcgagc ggctggcgtt gcagacccac cagttcgctc ccggtcgtat
      721 agacaccacc gtaccaagca acgtcgacct tggcgggcag ttgcagtgcc acaccgtcca
      781 gctgtgccga ttcaacctcc agccggaaac gcaagcccgc ctcgttggac tgcggcatgt
      841 ctgccaccac ccccaccacc atcacatcgc gcccctccag cgccggatcc agagcatgct
      901 gcaggaaaag cgcgctgcgc aagccggttg caccgaacgc cagtgccccc acggtcagtc
      961 ctgcggccag aaatccgggc agatttgcta tattttttgt agctgtatac gcatatccga
     1021 cgagggccaa ggccataaaa gccatataaa ccactggcga gccaaggtcc ggctgctgca
     1081 actgcaaggc agtacccgcc acccagccca gggtggcggc ggtcagggtg ccagtcacac
     1141 cggggaataa cgacctggaa tgcgttgcca tctgcatgtg tccatgctaa ccgggcatgg
     1201 gactggcgcg ggaattgctt tatgctgcac tagcactcta aagtcagcct atgtctatcc
     1261 acgccgcgct caaccacgtc acccactaca cctacgaccg gcttgtcaac cttggccccc
     1321 aggtgatccg cctgcgtcct gcgccccact gccgcagcaa ggtgatttcc tattcgctca
     1381 aggtcgaacc cagcacccat tttgtcaact ggcaacagga cccgttttcc aactaccagg
     1441 cgcgcctggt gtttcccgag aagaccaccg agttcaaggt cacggtcgat ctggtggtcg
     1501 aaatggcggt gtacaacccc ttcgacttct ttctggagcc tgaagccgaa gagttcccct
     1561 tcagctacga agccaacctc aagcaggaac tggcccccta cctgatcgcc gacccggtca
     1621 cacccatgtt gcagggtttt ctggacaaga tcgaccgcac caagcgccgc agcgtcatct
     1681 tcctggtgga cctgaacacc atggtgcaca aggcggtcaa ctacaccatc cgcatggagc
     1741 ccggcgtgca gacgccggaa gaaaccctct cactggccag cggctcctgc cgtgattcag
     1801 cctggctgat ggtgcaactg ctgcgcaact gcgggctggc tgcgcgcttt gtttccggct
     1861 acctgatcca gctgacaccc gatgtgaagt cgctcgacgg ccccagcggc accgaggtcg
     1921 acttcaccga cctgcacgcc tggtgcgagg tctatctgcc cggcgctggc tggattggcc
     1981 tggacgccac cagtggcctg ctggcgggcg aaggccatat tccgctggcc tgcacaccgc
     2041 agccctccgg tgcagcgccg atcgaaggcg gcgtggacga gtccgaggtg gaattcagcc
     2101 accacatgca ggtgacccgc atttacgaat ccccccgcgt caccaagcct tatacgcccg
     2161 agcaatgggc cgatgtcatg gcgctgggcg accaggtgga cgccgaactg gtggcgggcg
     2221 acgtgcgcct gaccatgggc ggtgagccca cgttcgtggc cgtcaacgac cgtgacgcgg
     2281 ccgaatggaa caccgacgcc ctgggcccga ccaagcgcgg ttttgccacc gcactggtgc
     2341 aaaagctgcg cgatgaatac ggtcagggtg gattcctgca ctttggccag ggcaagtggt
     2401 accccggcga gcaactgcca cgctgggcac tcaacatcta ctggcgtgcc gacaagcagc
     2461 cggtgtggcg caacccggca ctgttcaccg acgagcgcga ccccacccac tacaccagcg
     2521 aagacgccgg ttactttgca cgcaccctgg ccgccaagct gggccttacc gaccagtaca
     2581 tccagactgc gtatgaagac acctggtact acctgtggcg cgagcgtcgc ctgcccgtca
     2641 acgtagaccc gttcaattcc aaactcgacg acgagatgga acgcgcccgc ctgcgccgcg
     2701 tgttcgagca aaagctcgac acccccgtgg gctacgtgct gcccatcaag cccgccatcg
     2761 ccggcggtgg cccgcgcctg gccggcccgg ggtgggtcac cggcccctgg ttcttccgcg
     2821 atgagcgcat gtacctcatg cccggcgatt cgcccatggg cctgcgcctg ccgctggact
     2881 cgctgccctg ggtcagcaag ggggactttc cgtacctcat cgagcaggac ccctacgccc
     2941 cgcgcgatgc attgcctgcg catgatgctt ttgcacggcg ctacacaccc aactacgcca
     3001 gcccttccat caactccagc gctgcaccca ctgcacccgc aggcaatggt ggtggtgtcg
     3061 atggcatggc ctacctggcc ggtgccgtgc cacggcgtga agccacccgc aaactccaga
     3121 gcgccaccgg caacgccgtc aacaccgtaa ccgccaacgc gcaaagcacc acgcaggccg
     3181 agccggccaa ctttgcccgc gaacccaacc gcaatgaatc cgcccactgg atcacccgca
     3241 ccgcgctgtg tgtggaagtg cgcgacccgc gccgcgccag cggtcccaag gccgaggccg
     3301 tgggcaccaa atccggcgtc atgtatgtgt tcatgccacc gctggaacgt ctggaggact
     3361 acctggacct gctggccgcc atcgaggcca ccgccgagag cctgaagatg aagatcgtgc
     3421 tggaaggcta cccgccccca cgcgacccgc gcctcaagct gctgcaagtc acccccgacc
     3481 ccggcgtgat cgaagtgaac attcacccgg tgacgaactg gaaagaactg gtctacaaca
     3541 ccgagttttt gtacaacgcc gcgtttgagt cgcgcctgtc ggccgagaag ttcatgaccg
     3601 acggccgcca cacaggcacg ggtggcggca accactttgt gatgggcgga gccacgccgt
     3661 ctgacagccc cttcctgcgc aagccggaat tgctggcgag cctgctgctg tactggcaca
     3721 accacccgtc tctgagctat ttgttcagcg gcatgtttgt gggcccgacc agccaggccc
     3781 cgcgcgtgga cgaagcgcgc aacgaccagc tctatgagct ggaaattgcc atcgagcaga
     3841 tctacaagaa ccgcgaactc tacggccaga gcatgccgcc ctggttggtg gaccgcacgc
     3901 tgcgcaacat cctgatcgat gtgaccggca acacgcaccg cagcgagttc tccatcgaca
     3961 agatgtattc gcccgactcg tccactggcc gcctgggcct gctggagctg cgcgccttcg
     4021 agatgccacc gcacccgcac atgagcagcg tgcagcagct gctgctacgt g
//
LOCUS       DAOSJM010000219         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19558_length_4046_cov_5.362315, whole genome shotgun sequence.
ACCESSION   DAOSJM010000219 DAOSJM010000000
VERSION     DAOSJM010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19558_length_4046_cov_5.362315"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1441)
                     /locus_tag="PKX82_08495"
     CDS             complement(<1..1441)
                     /locus_tag="PKX82_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HOF52334.1"
                     /translation="MTTTHNYRPLHFGVTRGVLTAGEGGVQYLRAEQPLEPNAECMTD
                     RLVHWATTAPDRIWMAQRARNADGTKGDWRRISYAQALAGARSIAQAIVNRGLSAERP
                     VLILSENDLDHALLSLGCLLAGVPWCPASPAYSTISTDYSKLRHVYNTLTPGLVFASD
                     ASRYAKAIQSVVAPDVEVAFSNGSMEGRASTSFADLSATVATSAVDAAVAATGPDTIA
                     KFLFTSGSTKLPKAVINTQRMWCTNQQQMRQSMPVLAEQPPVLVDWLPWNHTFGGNHN
                     FGLVVYNGGTMYIDDGKPVPALIGETLRNLREIAPTVYFNVPTGFEAIASAMKTDLAL
                     RKNFLSQARMFFYAGAALAQPIWDSLFESEEAEIGQRIVMGTGLGMTESGPFGIFVTN
                     PDVRSGDLGLPAPGLELKLVPSEGKLEVRYRGPNITQGYWRAPEETAAHFDSEGFFCT
                     GDAVKWIDETNIHLGLKFDGRIAEDFKLAT"
     gene            complement(1469..2251)
                     /locus_tag="PKX82_08500"
     CDS             complement(1469..2251)
                     /locus_tag="PKX82_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOF52335.1"
                     /translation="MPSKDIQLEICGDNQEVAVIRLTRGKKRNALNDALIISIRNIFE
                     NLPDTVRAAMIDGEGEHFCAGLDLSELKERDAGQGLHHSRMWHAALERVQYGPVPVIA
                     ALHGAVVGGGLELASACHIRVADESTFYALPEGTRGIFVGGGGAVRIPRLIGTARMTD
                     MMLTGRVYNAQDGERIGMAQYLVPTGTAFDKAFELAQRIATNAPLTNYALMHALPRIA
                     EQPADQGFFTEAMMAAIAQSAPEAKERVKAFLDGKAAKVKKA"
     gene            complement(2402..2725)
                     /locus_tag="PKX82_08505"
     CDS             complement(2402..2725)
                     /locus_tag="PKX82_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HOF52336.1"
                     /translation="MASSPSKEIYILGLTHQGKTFRPSDWAERLAGVMSQFRPGGPQP
                     GSHLGYSPWCIPRSFGPVKCVIVHSDLRDYDVMAWDFCINFAKDNDLQTSDTPPVVPE
                     RPANP"
     gene            complement(2852..3577)
                     /locus_tag="PKX82_08510"
     CDS             complement(2852..3577)
                     /locus_tag="PKX82_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52337.1"
                     /translation="MTAFFRPIACSLLAAMSLATGLPAWAADPVTDAMQQAYGPYRAA
                     LFKTNSGSAAESQQAVTQAQTSWAQIVKQFGSKPAAPYDRDTRFAASLSEVTAVYAKA
                     AEEIGRNQLAAAHETLERARDVMAEMRQRNNIVVFSDYMNAYHAQMEHVLVEGAKLFD
                     QPKGLLQLTAQVGVLEYLAAQLGTQAPASQASDPEFVAALKVVQQSVAELKAALLAQD
                     PVAVKAAIGKIKGPYSKMFLKFG"
     gene            3760..>4046
                     /locus_tag="PKX82_08515"
     CDS             3760..>4046
                     /locus_tag="PKX82_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF52338.1"
                     /translation="MTAATPATTPSSPHVMNTYGRLPIAMSHGQGCRVWDTHGREYLD
                     ALGGIAVNTLGHNHPKLVPALQDQIAKLIHTSNYYHVPNQEVLAQMLVERSG"
BASE COUNT          783 a         1240 c         1215 g          808 t
ORIGIN      
        1 cggtggcgag cttgaagtcc tccgcaatgc ggccatcgaa cttcaggccc agatggatgt
       61 tggtttcgtc aatccatttg accgcatcac ccgtgcagaa aaaaccttcg ctgtcaaagt
      121 gtgcggcggt ctcctcgggt gcacgccagt agccttgggt gatgttgggg ccgcgatagc
      181 gcacctccaa cttgccctca ctgggtacca gcttgagttc gaggccgggg gccggcagac
      241 ccagatcgcc agagcggaca tcggggttgg ttacaaaaat tccaaagggt ccagactcgg
      301 tcatgcccag gccggtaccc atcacgatgc gctggccaat ctcggcctct tcgctctcga
      361 aaaggctgtc ccaaatcggc tgcgccagag cagcaccggc gtagaagaac atgcgcgcct
      421 gggaaagaaa gttcttccgc agcgccagat ccgtcttcat cgcgctggcg atcgcttcaa
      481 agcccgtagg cacattgaaa tacaccgtgg gcgcaatctc acgcagattg cgcaacgttt
      541 ccccgatcag cgcgggcacc ggcttgccat cgtcgatgta catcgtgccg ccgttgtaga
      601 ccaccaggcc gaaattgtgg ttgccgccaa aggtgtggtt ccagggcagc cagtccacca
      661 gcaccggggg ctgctcggcc agcaccggca tgctctggcg catttgctgc tggttggtac
      721 accacatgcg ctgggtattg atcaccgcct tgggcagttt ggtggatccg ctggtaaaca
      781 gaaacttggc aatcgtgtcc ggcccggtgg ctgcaacggc tgcatccact gcggacgttg
      841 ccacagtggc agagaggtca gcaaacgatg tgctcgcacg cccttccatg ctgccgttgg
      901 aaaaggccac ttccacgtcg ggtgcaacca ccgactggat cgccttggcg tagcggctgg
      961 cgtcgctggc aaacaccaga cctggggtca gcgtgttgta gacatggcgc agcttggagt
     1021 aatcggtgct gatggtggaa tacgcgggcg acgccggaca ccagggcaca ccggcgagca
     1081 gacaacccaa agacagcagg gcgtggtcca gatcgttctc gctcaggatc agcacaggcc
     1141 gctctgcact caagccgcga ttcacaatcg cctgggcaat gctgcgcgcg ccggccagcg
     1201 cctgcgcgta gctgatgcga cgccagtccc ccttggttcc gtcggcattg cgggcacgct
     1261 gggccatcca gatgcggtcc ggcgcagtgg tggcccagtg caccagccgg tcggtcatgc
     1321 attcggcgtt tggctccagt ggctgctcgg cgcgaaggta ttgcaccccg ccctcgccgg
     1381 cggtgaggac accccgggtc accccgaagt gcaatggccg gtaattgtgt gttgttgtca
     1441 tttgtcttgt ctcctgcgct gatcgttgtc aggctttttt gactttggcg gccttgccat
     1501 ccagaaaggc cttcacgcgc tccttggctt ccggtgcact ttgggcaatg gcggccatca
     1561 tggcttcggt gaagaagccc tggtcagcgg gctgctcggc aatgcgtggc agcgcgtgca
     1621 tcagcgcgta gttggtcagc ggcgcgttgg tggcgatgcg ctgggccagc tcgaacgcct
     1681 tgtcaaaggc agttccggtg ggcaccaggt actgcgccat gccaatgcgc tcgccgtcct
     1741 gcgcgttgta cacgcggcca gtcaacatca tgtcggtcat gcgggccgtg ccaatcaggc
     1801 gtggaatgcg caccgcaccg ccgccgccca caaaaatgcc gcgggtgcct tccggcaagg
     1861 cgtaaaaggt ggattcgtcg gccacacgga tgtggcaggc gctggccagt tccaggccgc
     1921 cacccaccac tgcgccgtgc agtgcggcaa tcaccggcac ggggccatac tgcacccgct
     1981 ccagcgcggc atgccacatg cgggaatggt gcaggccctg gcccgcatcg cgctccttga
     2041 gctcggacag atccagacca gcgcagaagt gctcgccctc gccatcaatc atcgcggcac
     2101 gcacggtatc gggcaggttt tcaaagatgt tgcggatgga aataatgagc gcatcgttga
     2161 gcgcattgcg cttttttccg cgcgtcaggc gaataacggc aacctcctgg ttgtcgccgc
     2221 atatttcgag ttggatgtct ttggatggca tagcgggttt cctcaatttg gtcgaattat
     2281 ggttataaaa aataaccaat tcaacccacc cagccgctta ttttctaggt gtttacccgg
     2341 ccatcacgcg cgcgcgagca cgccgctcca gccactggag cagacactaa ccaaaggaaa
     2401 atcaggggtt ggccggacgc tcgggaacca caggcggcgt atcgctcgtc tgcaagtcgt
     2461 tgtctttggc gaagttgatg cagaagtccc acgccataac atcgtagtcg cgcagatcgc
     2521 tgtggacgat gacgcacttc actggaccaa acgaccgggg aatgcaccat ggcgaataac
     2581 ccagatggct gcctggctga ggcccacccg ggcgaaactg actcatcacg ccggcaaggc
     2641 gttcggccca gtcgctgggg cgaaaggtct tgccctggtg tgtcagcccc aggatgtaga
     2701 tctctttgga aggtgaggag gccatcagtt ggaagttagt cgcagcgtct ggtcggaagc
     2761 agcctgttgg gctgctgcgc cgcagcatgg gccggattct atctgagaca agcctccaat
     2821 ttactgacac ccgcggcggt ggggatactc ctcagccgaa cttcagaaac atcttgctgt
     2881 aaggcccctt gatctttccg atggctgcct ttacagctac tgggtcctgt gccagcaggg
     2941 ctgccttcaa ctcagccaca gattgctgca caactttcag ggcagccaca aactccgggt
     3001 cggaagcctg tgacgccggt gcctgtgtac ccaactgtgc tgccaggtat tccagcacac
     3061 cgacctgcgc ggtcaactgc agcaatccct tgggttggtc gaacagcttt gcgccttcta
     3121 ccagcacgtg ctccatctgt gcgtggtagg cattcatgta gtcactgaac acaacgatgt
     3181 tgttacgctg ccgcatttct gccatgacgt cgcgcgcacg ctccagggtt tcgtgggcgg
     3241 cggccaactg gttcctgcct atctcttcgg ccgccttggc gtacaccgca gtcacttcgg
     3301 acagcgaggc cgcgaaccgg gtgtcgcggt cgtagggagc agcaggcttg ctgccaaact
     3361 gcttcacgat ctgtgcccag gatgtctggg cctgggtgac tgcctgttgc gactcggcgg
     3421 ctgatccact gttggttttg aacagtgctg cacggtaggg gccatacgct tgctgcatgg
     3481 catcggtcac cggatcggcc gcccaagcag gcaggccagt agccagcgac atggctgcca
     3541 acaaggaaca cgcaatgggg cgaaagaatg ctgtcatgga agggaatttc tatataagtg
     3601 actggcgaag tttaatcttc aaaccacccc gtcgacaagc cgccagacct gaacacggag
     3661 ggtgcgccgg cgatggccct aaaattacct ttcaacggcc caacctgcaa agccattgcg
     3721 ttgggccgac ttgtttttgt gcaacccctg gagcttcaca tgaccgccgc cacgccagcc
     3781 accacgccgt cttcacccca cgtgatgaac acctatggcc gcctgcccat tgccatgtcg
     3841 cacggtcagg gttgccgcgt gtgggacacc catggcaggg agtatttgga tgcattgggt
     3901 ggcattgcgg tcaacaccct gggccacaac caccccaaac tggtgccggc actgcaggac
     3961 cagatcgcca agctgatcca cacctccaac tactaccacg tgcccaatca ggaagtgctg
     4021 gcccagatgc tggtggaacg ctccgg
//
LOCUS       DAOSJM010000220         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19561_length_4046_cov_3.979955, whole genome shotgun sequence.
ACCESSION   DAOSJM010000220 DAOSJM010000000
VERSION     DAOSJM010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19561_length_4046_cov_3.979955"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>349)
                     /locus_tag="PKX82_08520"
     CDS             complement(<1..>349)
                     /locus_tag="PKX82_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52339.1"
                     /translation="KKNKIAFFHGRGSFVKVAEGGYDIKVAGAAEEVITGKQIIIATG
                     SNARALPGTPFDEVNVLSNDGALNIGAVPKKLGLIGSGVIGLEMGSVWKRLGSEVTIL
                     EGLPVFLGAVDEGV"
     assembly_gap    350..359
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<360..696)
                     /locus_tag="PKX82_08525"
     CDS             complement(<360..696)
                     /locus_tag="PKX82_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52340.1"
                     /translation="MSKQFDVIVIGGGPGGYIAAIRAAQLGFNVACIDEWKNAAGGPA
                     PGGTCTNVGCIPSKALLQSSEHFNHANHHFAEHGIEVKGVSMDVAKMIARKNSVVKQN
                     NDGILYLLKK"
     gene            complement(706..1965)
                     /gene="odhB"
                     /locus_tag="PKX82_08530"
     CDS             complement(706..1965)
                     /gene="odhB"
                     /locus_tag="PKX82_08530"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOF52341.1"
                     /translation="MAIVEVKVPQLSESVAEATLLQWKKKVGDAVAVDEILIEVETDK
                     VVMEVPAPSAGVLVELVVADGATVAAEQLIARIDTEGKAGAAAPVAPIVAASAALAAA
                     PAAAAAMPASASKAGVAMPAAAKLMADNGMAAGSVAGTGKDGRVTKGDVLQAASAPKV
                     VAVSPIPTGVKAASLPQVAAPTVNLGDRPEQRVPMSRLRARVAERLLQSQSSNAILTT
                     FNEINMAPVMDMRKRMQDKFEKEHGVKLGFMSFFVKAAVHALKKFPVLNASVDGNDIV
                     YHGYFDIGIAVGSPRGLVVPILRNADQMSFADIEKKIAEFGQKAKDGKLGIEEMTGGT
                     FSISNGGTFGSMMSTPIINPPQSAILGVHATKDRAMVENGQVVVRPMNYFAMSYDHRI
                     IDGREAVLGLVAMKDALEDPARLLFDI"
     gene            complement(1978..>4046)
                     /locus_tag="PKX82_08535"
     CDS             complement(1978..>4046)
                     /locus_tag="PKX82_08535"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOF52342.1"
                     /translation="MAHRGRLNVLVNTLGKMPGDLFAEFDHTAPEELPAGDVKYHQGF
                     SSDVSTLGGPVHLSLAFNPSHLEIVNPVVEGSVRARMDRRADPLGKQVLPVLVHGDAA
                     FGGQGVNQETLCLAQTRGYTTGGTVHIIINNQIGFTTSDPRDMRSSAFCTDIVKMVES
                     PVLHVNGDDPEAVVLATQLALEFRMEFRQDVVVDITCFRKLGHNEQDTPALTQPLMYK
                     KIAAHPGTRKLYADKLATQGLGETLGEDMVKAYRAAMDAGKHTVDPVLTNFKSKYAVD
                     WSPFLGKKWTDAGDTAIPLTEWKRLSEKLTAIPASVNPHSLVKKVYDDRAAMGRGDVN
                     VDWGMGESMAFASLVASGYPVRLSGEDSGRGTFTHRHAVVHDQAREKWDAGTYVPLQN
                     VAENQAPFVVIDSILSEEAVLGFEYGYASNDPNTLVIWEAQFGDFANGAQVVIDQFIA
                     SGEVKWGRVNGITLMLPHGYEGQGPEHSSARVERFMQLAADTNMQIVQPTTASQIFHV
                     LRRQMVRNLRKPLVIFTPKSLLRNKDATSPLSEFTKGGFQTVIPESKALKADKVKRIV
                     ACSGKVYYDLVKKRDEKGADDVAILRVEQLYPFPHKAFATELKKYPNATEVVWCQDEP
                     QNQGAWFFVQHYLHENMVEGQRLGYSGRAASASPAVGYSHLHQEQQKALVEGAFGKLK
                     GFVLSK"
BASE COUNT          769 a         1248 c         1181 g          838 t
ORIGIN      
        1 gacgccttcg tccacggcac ccagaaacac tggcagacct tcgaggatgg tgacttcaga
       61 acccagacgc ttccacaccg agcccatttc cagaccgatc acgccggagc cgatgaggcc
      121 cagcttcttg ggcaccgcgc cgatgttcaa agcgccatcg ttggacagca cgttgacttc
      181 atcgaacggg gtaccgggca aagcgcgcgc gttggaaccg gtggcaatga tgatctgctt
      241 gccggtgatg acttcttcgg cggcgccagc caccttgatg tcgtagccgc cttcagcgac
      301 tttcacaaac gagccacggc catggaagaa agcaatcttg ttcttcttgn nnnnnnnnnt
      361 cttcttgagc agataaagga taccgtcgtt gttctgcttg accaccgagt tcttgcgggc
      421 aatcatcttg gccacatcca tgctcacacc tttcacctca atgccatgct cggcaaagtg
      481 gtggttggca tggttgaagt gctcggacga ctgcagcagc gccttggaag gaatgcagcc
      541 cacgttggtg caggtaccgc cgggcgcagg tccgccagcg gcgttcttcc actcgtcgat
      601 acaggccacg ttgaagccca gctgggcagc gcgaatggca gcgatatagc cgccggggcc
      661 gccgccaatg acgatcacgt caaattgttt gctcatcgtt ggtccttaga tgtcaaacag
      721 caggcgtgcc ggatcttcca gtgcatcctt catggccacc agacccagaa cggcttcgcg
      781 gccgtcgatg atgcggtggt cgtaggacat ggcgaagtag ttcatggggc gaaccaccac
      841 ctggccgttt tccaccatcg cgcggtcttt ggtcgcgtgt acgcccagaa tcgcgctttg
      901 cggcgggttg atgatggggg tggacatcat cgagccgaaa gtgccgccat tggagatgga
      961 gaacgtacca ccggtcattt cttcaatgcc cagtttgccg tccttggcct tctggccgaa
     1021 ttcagcaatc ttcttctcga tgtccgcaaa gctcatctgg tcagcattac gcagaattgg
     1081 caccaccagg ccacggggcg agcccacggc gataccgata tcaaagtagc cgtggtagac
     1141 gatgtcgttg ccgtccacgg acgcgttcag gaccgggaac ttcttcaggg cgtgcactgc
     1201 ggccttgacg aagaagctca tgaagcccag cttgacgccg tgctccttct caaacttgtc
     1261 ctgcatgcgc ttgcgcatgt ccatgaccgg agccatgttg atttcgttga aggtggtcag
     1321 gatggcgttg ctgctttgcg actggagcag acgctcggct acacgggcac gcagacggct
     1381 catgggcacg cgctgctcag ggcgatcacc cagattgact gtgggcgcag ccacttgagg
     1441 caaggatgcc gctttaaccc ccgtggggat tggggaaact gctactactt taggagcaga
     1501 tgcagcctgc agcacatccc ccttggtcac gcggccatct ttaccggttc ctgcaacgga
     1561 acctgcagcc atgccgttgt ctgccatcag cttggcagcc gcgggcattg ccacgccagc
     1621 cttggaagca ctggccggca tcgctgctgc cgcggccggt gctgcggcca gcgctgcgga
     1681 tgcagcaacg atgggcgcca ctggtgccgc agcgcccgct ttgccttcgg tgtcgatccg
     1741 ggcaatcagt tgctcagcag ccacggtggc gccatctgcc acgaccagtt cgacaagaac
     1801 acccgccgat ggggcgggca cttccatgac cactttgtcg gtttcgactt cgatcaggat
     1861 ctcgtcgacc gccacagcgt cgccgacctt cttcttccac tgcaacaggg ttgcttcggc
     1921 cacagattca gacagctgcg gtacttttac ttctacgata gccataacaa ttcttcctta
     1981 tttggacagg acaaagcctt tgagttttcc gaaagcacct tccaccagag ccttttgctg
     2041 ctcctggtgc aagtgcgaat agcccactgc aggcgatgcc gatgcggcac ggcccgagta
     2101 acccaaacgc tgtccttcga ccatgttctc gtgcaggtaa tgctgcacaa agaaccacgc
     2161 gccctggttt tgcggctcgt cctggcacca caccacttcc gtggcgttgg ggtacttctt
     2221 caactcggtc gcaaacgcct tgtgcgggaa cggatacaac tgctcgacgc gcagaatggc
     2281 gacgtcgtcg gcacccttct cgtcccgctt cttgaccagg tcgtaataca ccttgccgga
     2341 gcaggccaca atgcgcttga ccttgtctgc cttgagcgcc ttgctctcgg gaatcacggt
     2401 ctggaagcca cctttggtga actccgacag cggagacgtc gcatccttgt tgcgcaacag
     2461 ggacttgggc gtgaagatca ccagcggctt gcgcaggtta cgcaccatct ggcgacgcaa
     2521 cacgtggaag atctggctgg ccgtggtcgg ctgcacgatc tgcatgttgg tgtccgccgc
     2581 cagctgcatg aaacgctcga cgcgggccga actgtgttcc gggccctggc cttcatagcc
     2641 gtggggcagc atcagcgtga tgccattcac gcggccccac ttcacttcac ccgaagcaat
     2701 gaactggtca atcaccacct gggcgccgtt ggcgaagtcg ccaaactgcg cttcccagat
     2761 caccagggtg ttgggatcgt tggaggcgta gccgtattca aaaccgagta cggcttcttc
     2821 tgacaggatg gagtcgatga cgacaaacgg tgcctggttc tccgccacgt tttgcaacgg
     2881 aacataggta ccggcatccc atttttcgcg cgcctggtca tggaccacgg cgtggcggtg
     2941 ggtgaaggtg ccgcgaccac tgtcctcacc cgacaggcgc accgggtagc cactggccac
     3001 cagggaggca aaggccatgc tctcgcccat gccccagtcc acattgacgt caccacggcc
     3061 catggcggcg cggtcgtcat agaccttttt caccagggag tgcggattca cgctggcagg
     3121 aatggctgtg agcttctcgg acaaccgttt ccactcggtc agcggaatgg cggtatcacc
     3181 cgcgtcagtc cacttcttgc cgaggaacgg agaccagtcc accgcgtact tgctcttgaa
     3241 gttggtcagt accggatcaa ccgtgtgctt gccagcatcc atggcggcac ggtaggcctt
     3301 gaccatgtct tcgcccaacg tctcgcccag cccctgggtg gccagcttgt cagcgtacag
     3361 cttgcgggta ccagggtgcg cagcaatctt cttgtacatc agcggctgcg tcagagccgg
     3421 cgtatcctgc tcgttgtggc ccagtttgcg gaagcaggtg atgtccacca ccacgtcctg
     3481 gcggaactcc atgcggaatt ccagagccag ctgggtcgcc aggaccacag cttcggggtc
     3541 atctccattc acgtgcagaa cgggagactc caccatcttc acgatatcgg tacagaacgc
     3601 actggagcgc atgtcacgcg ggtccgaggt ggtgaagccg atctggttgt tgatgatgat
     3661 gtgcaccgtg ccgccggtgg tgtagccacg ggtctgggcc aggcacaggg tttcctggtt
     3721 gacgccttgt ccgccaaatg ctgcatcgcc gtgcaccagc accggcaaca cctgcttgcc
     3781 cagcgggtcg gcgcggcggt ccatgcgggc gcgcaccgaa ccttccacca ccgggttgac
     3841 gatttccagg tgagacgggt tgaacgccag tgagaggtgc accggaccac ccagggtgga
     3901 tacgtccgaa ctgaaaccct ggtggtactt cacgtcgccg gctggcaact cttctggagc
     3961 cgtatggtca aactcggcaa acaggtcacc cggcatcttg cccagggtgt tgaccagcac
     4021 gttcagacgg ccacggtggg ccatgc
//
LOCUS       DAOSJM010000221         4042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19594_length_4042_cov_4.415099, whole genome shotgun sequence.
ACCESSION   DAOSJM010000221 DAOSJM010000000
VERSION     DAOSJM010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19594_length_4042_cov_4.415099"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1209
                     /gene="purF"
                     /locus_tag="PKX82_08540"
                     /pseudo
     CDS             1..1209
                     /gene="purF"
                     /locus_tag="PKX82_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835511.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
     gene            1213..2427
                     /locus_tag="PKX82_08545"
     CDS             1213..2427
                     /locus_tag="PKX82_08545"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HOF52343.1"
                     /translation="MSDKQLPAGLHPATLAVREAVPPSQYGENSEALYLTSGYIQPTA
                     EVSAARFAGEEDGYTYGRSGNPTVTSFERRLAAMEGAEACMATSSGMSAIMLMCFSLL
                     KSGDHVVFSQSMFGSTIKLIGSEFARFGVESTIVPQTDLAAWKAAVRPNTRLLFAETP
                     TNPLTELCDIAALAEIAHQAGALLAVDNCFATPVLQQPLRLGADIVMHSGTKYLDGQG
                     RVMAGALCASEAMVRDKFLPVQKNSGMVLSPFNAWVVLKGLETLDLRMKAQSAQTLQL
                     AQWLESHPGVARVYYPGLVSHPQHALAQQQMGGLGGAVLSFDVKASSPEQARARAFHV
                     LDSMQVLSLCTNLGDTKTLCTHPASTSHGKLAEAQRQAAGIGQGLIRVAVGLEHLDDI
                     KNDLLRGLDTLA"
     gene            2424..3827
                     /gene="gltX"
                     /locus_tag="PKX82_08550"
     CDS             2424..3827
                     /gene="gltX"
                     /locus_tag="PKX82_08550"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOF52344.1"
                     /translation="MTQKIRTRIAPSPTGFLHLGTARTALYSWAYARHFGGEFVLRIE
                     DTDVARSTQDSVDQILESMRWLGLEYDEGPIYQMQRLDRYKAVVDQLMAEGKAYYCYS
                     TPEELDAVREAKKARGEKALYDGRWRPAPGKTLPPVPAGVKPVVRFCNPQEGDVTWND
                     LVKGPITISNREIDDLIIVRTDGIPTYNFAVVVDDWDMQISHVFRGDEHINNTPWQIN
                     IFAALGAPLPLFGHCPVILGDDGQKLSKRRGAVSVTAYEEGGYLPEAMLNYLARLGWS
                     HGDEELFTRAQLVEWFDGTHLNKSPAQWDPVKLLWVNAQYIKQADNTRLAGLVAQQLT
                     RQGITLDASVVQTHLPQVCALLKDRCDTTVALATWAAKFYADVTPDAAELAQHVTDAV
                     KPALQALAARLATCTWDKASIAAAIKEVLAEVGLKMPQLAMPVRVLVLGTPQTPSVDA
                     VLELLGREKVLARLGRA"
     gene            3904..3979
                     /locus_tag="PKX82_08555"
     tRNA            3904..3979
                     /locus_tag="PKX82_08555"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3937..3939,aa:Ala,seq:ggc)
BASE COUNT          825 a         1222 c         1229 g          766 t
ORIGIN      
        1 gctggtgcac aacggcaacc tgaccaatgc gcacgccctc aaggccgagc ttttcaatac
       61 cgaccaccgc cacatcaaca ccgaaagcga ttcggaagtg ttgctcaatg tgctggcgca
      121 cgagattgaa atcactacgc gtggtgccac gctgcaggtc gaaaatctgt tcgctgcggt
      181 ggccaatgtg caccgccgcg tcagggggtc gtacgcggtg attgcgctga ttgccggcca
      241 tggcgtactg gctttccgcg acccctttgg cattcgcccc ttgtgcatgg gaaagagtgc
      301 ggacggcacg gtcatgctgg ccagcgagtc ggtgacgctc gaaggcactt cgcaccagtt
      361 cgaccgcaac atcgaccccg gtgaagccat cttcatcgac ctgcagggca atgtgcacgc
      421 caagcagtgc gccgatgctc ccaccctcaa gccctgtatt tttgagtttg tgtacctggc
      481 ccgtccggac tcggtcatgg atggcatctc ggtgtaccag gcccggctga atctgggtga
      541 gaccctggcc aagcggtgat ttccaccgtg ccaccgaacg aaattgacgt cgtgatcccg
      601 attcccgaat ccagccgccc cagcgcggcg caactggcgc aactgctggg tttgccgtac
      661 cgcgaaggtt ttgtgaagaa ccgctatgtg ggccgcacct tcatcatgcc gggtcaggcc
      721 gtgcgcaaga aatccgtgcg ccagaagctc aacgtgattg ccagcgaatt caagggccgc
      781 aatgtgttgc tggtggatga ctccatcgtg cgcggaacga ccagcaagga aatcgtgcag
      841 atggcgcgcg atgccggcgc ccgcaaggtg tatatggcca gcgctgcacc gcccgtgcgt
      901 taccccaatg tgtatggcat cgacatgccg accagcaacg aactggtggc gcacaaccgc
      961 accgtggagc agatccgcga aatcatcggc tgcgatgcgc tgatctacca ggacgtggac
     1021 ggcatgaaga aggcgattgg ctcgctgagt accaagctgg cgggttttga tgcctcatgt
     1081 tttgatggtg tgtacgtcac cggcgatatc accagtgatg atattgaccg tttgaactcc
     1141 aaccgtgtgg gtggtgatga gtcgcaggaa gacacctcgc gcctggcgct gcccaaccac
     1201 agcgagtaag ccatgagtga caagcaacta cccgctggtc tgcacccggc caccctggca
     1261 gtccgcgaag ctgtgccacc cagtcagtac ggcgaaaact ccgaagcgct gtacctgacc
     1321 agcggctata tccagccgac ggcggaggtg tcggccgccc gttttgcggg cgaggaagat
     1381 ggctacacct atggccgcag tggaaacccc acggtcacca gcttcgagcg acgactggca
     1441 gccatggaag gcgccgaggc ttgtatggcc acgtcaagcg gcatgtcggc catcatgctg
     1501 atgtgcttca gcctgctcaa gtccggcgac catgtggtgt tctcgcagtc gatgtttggc
     1561 tccaccatca agctcattgg cagcgagttc gcacgctttg gtgtggagtc caccatcgtc
     1621 ccgcagaccg atctggctgc ctggaaggcg gcggtgcgac cgaatacgcg tctgttgttt
     1681 gccgagacgc ccaccaaccc gctgacggag ttgtgcgata tcgctgcgct ggccgagatt
     1741 gcccaccagg ccggtgcgct gctggccgtg gacaactgct ttgccacgcc ggtgctgcaa
     1801 caacccctgc gtctgggtgc tgacatcgtg atgcattccg gcaccaaata cctggacgga
     1861 caggggcgtg tgatggctgg tgcactgtgc gccagtgaag ccatggtgcg cgacaagttc
     1921 ctgccggtgc agaagaactc cggcatggtg ctgtcccctt tcaatgcatg ggtcgtgctg
     1981 aaaggcttgg aaacgctgga cctgcgcatg aaagcccaga gcgcacaaac cttgcagctg
     2041 gcacagtggc tggaatcgca tccgggcgtg gcgcgtgtct actacccagg gcttgtcagc
     2101 catccgcaac atgcactggc ccagcagcag atgggcggac tgggcggagc cgtgctgtcg
     2161 tttgacgtga aggcgtcctc cccagagcag gcacgtgcac gtgccttcca tgtgctggac
     2221 tcgatgcagg tgctttcgtt gtgcaccaac ctgggcgaca ccaaaacgct gtgtacccat
     2281 ccggcgagta catcgcacgg caaactggcc gaggcgcagc gccaggccgc cggcatcggg
     2341 caaggcctga tccgtgtggc ggtggggttg gaacacctcg acgacatcaa gaacgaccta
     2401 ttgcgcggcc tggatacatt ggcatgacac agaaaattcg cacccgtatt gcaccatcac
     2461 ccactggctt cctgcacctg ggcaccgccc gcaccgcctt gtattcctgg gcctatgccc
     2521 gccattttgg tggcgaattt gtgctgcgca tcgaagacac cgacgtggcc cgctccacac
     2581 aggattcggt cgaccagatt ctggaatcca tgcgctggct ggggctggag tacgacgaag
     2641 gcccgatcta ccagatgcag cgtctggacc gttacaaggc agtggtcgat cagctgatgg
     2701 ctgagggtaa ggcctactac tgctacagca cgcccgaaga actggacgcc gtgcgcgaag
     2761 ccaagaaggc ccgtggcgaa aaagccttgt acgacggccg ctggcgtccc gcgcccggca
     2821 agactttgcc gcccgtgccc gcgggtgtga agcccgtggt gcgcttctgc aatccgcagg
     2881 agggcgatgt gacctggaac gatctggtca agggccccat caccatcagc aaccgcgaga
     2941 tcgatgacct catcatcgtg cgcaccgatg gcattcccac ctacaacttt gcggtggtgg
     3001 tggacgattg ggacatgcag atcagccacg tgttccgcgg tgacgagcac atcaacaaca
     3061 cgccgtggca aatcaacatc tttgctgcgc tgggcgcacc cctgccgctg ttcggccatt
     3121 gcccggtcat cctgggcgac gatggacaaa agctgtccaa gcgccgtggt gccgtgagcg
     3181 tgaccgccta cgaagaaggc ggctacctgc ccgaggccat gctcaactac ctggcccgcc
     3241 taggctggag ccacggcgac gaagaactct tcacccgcgc ccagttggtg gaatggttcg
     3301 atggcacgca cctgaacaag agccccgcgc agtgggatcc ggtcaagttg ctatgggtga
     3361 atgcccagta catcaaacag gcggacaaca cgcgtctggc cggtctggtg gcgcaacaac
     3421 tgacacgcca gggaatcact ctggacgcct cggtggtgca aacgcatttg ccgcaggtct
     3481 gcgccttgct caaggaccgt tgcgacacca ccgtggcact ggccacatgg gctgccaagt
     3541 tctacgccga cgtcaccccc gacgctgccg aactggccca gcacgtgacc gacgcggtca
     3601 aacccgcctt gcaggccctg gccgccaggc ttgcgacctg cacatgggac aaggccagca
     3661 ttgccgcggc catcaaggaa gtgctcgccg aagtcggttt gaagatgcca caactggcca
     3721 tgccggtgcg cgtactggta ttgggcacac cccagacgcc atcggtggat gcggtgctgg
     3781 aattgctcgg tagagaaaaa gtattggcgc gacttggcag ggcatgattt ttaaggctat
     3841 aatttgaggc tcggaaattg agaatgtttt aaaaagcatt ctcgcgatta ctgaaaagta
     3901 attgggggta tagctcagct gggagagcgc ttgcatggca tgcaagaggt cagcagttcg
     3961 atcctgctta cctccaccaa gatttctgag aacggattag tccaaggttt tgaccccatc
     4021 gtctagaggc ctaggacatc ac
//
LOCUS       DAOSJM010000222         4040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19609_length_4040_cov_3.520703, whole genome shotgun sequence.
ACCESSION   DAOSJM010000222 DAOSJM010000000
VERSION     DAOSJM010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19609_length_4040_cov_3.520703"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PKX82_08560"
     CDS             <1..734
                     /locus_tag="PKX82_08560"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOF52345.1"
                     /translation="KGWMNLTQKVQWDVDGIEGLDPRSWYLVNSNHQSWVDILVLQYL
                     LNRRIPLLKFFIKQQLIWVPVMGLAWWALDFPFMRRHSEEFLRKHPEMRGKDQETTRK
                     ACAKFALIPTSVMNFLEGTRFTVAKHAKQQSPYTHLLKPKAGGMALALNAMGDKFRAI
                     LDVTIVYPDGVPTFGEFLSGKLRRVVVRVRRLPIPEELLHGDYGQDAAFREAFSAWVQ
                     QLWRDKDAQISALLLASRPAGNKSP"
     gene            complement(761..1705)
                     /locus_tag="PKX82_08565"
     CDS             complement(761..1705)
                     /locus_tag="PKX82_08565"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF52346.1"
                     /translation="MFELRHLRHALGLAEHRNFARAAEAMHISQSALSRSIQSLEESL
                     EVILFDRSHGGVEPTIFGQLLLKNARDLDLAARDLQREIELAKGLSSGELKIGVGPWG
                     AAVLAGAVVGAMSLQYPHLTVNLVICPWKEMPARLNTREIDLAIINSSDVPVDDEIEV
                     QPLLKHPSLLVCRANHPLSKFAAPTARDVFAFPLAAPNLPSGAIEQVLSHLPAELRAV
                     VLKRGVMRITCDSSSVLKSVIFHSDALTVMNAFMVLEELRSGKLITIPGINLGVGGQF
                     GVVRLRRRTLSVAAQRFIDLLFERDAELALEEEAYLGK"
     gene            1846..3270
                     /locus_tag="PKX82_08570"
     CDS             1846..3270
                     /locus_tag="PKX82_08570"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HOF52347.1"
                     /translation="MGAGLAGLTPGLSLAQEEKKVHPAQLDMLKGQEGVGNPREAHAY
                     AIGMQAFEYGFPLVYMASLSYTFNKDPMGQQVPLNAFISPKDFVATSNFRNGGSFNTD
                     TVYAGAFIDLRKGPMVLTTQDPEGHYFSVQLSDLYTNNFAYISKRSGGPIFGNFLLVP
                     PGWTGKIPKGKFDRVLQCEQKWLFALVRLRIDQDDAAEVTWAKAKFKQAQFTPMDDFL
                     AGRTFTPNDNNVFDVTQFKGNPLRPFAILNHMLTENPPPATDEILLQGFKTVHIGPGM
                     DLSKNDPDTLRGLARAARDGLPALRSHVEHPWARSVNGWFYFPTNIGQAKTTDYIMRS
                     AWQSLWGIVAHPPRECTYLWASQDQNGERLNSNGKYELYMRKDQFPKVEAFWSFTMYQ
                     DFNLVVNDANRWAIRENMKGLKFDADGGLRIYIQTERPSEDKVSNWLPAPKSGNFNIT
                     MRNYMPSQEIVEQRYDPPVMKRVS"
     gene            3438..>4040
                     /locus_tag="PKX82_08575"
     CDS             3438..>4040
                     /locus_tag="PKX82_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52348.1"
                     /translation="MKKISYMLLAACTVLAAGCSKNTGVGGAAGEATPATIQANTQLA
                     KDLKLGDQQDFEDAKRGFIARPNGKVLSADGTVLHDYDAYQFIEGQAPDTVNPSLWRH
                     ALLNAQMGLFKVTDGVYQLRGFDIANITLIEGKSGWIVVDALTSRESASAAMAFARKH
                     LSDKPVTALIFTHSHADHFGGALGIITPEEVAKRKVPVLAS"
BASE COUNT          938 a         1204 c         1103 g          795 t
ORIGIN      
        1 acaagggctg gatgaacctc acgcaaaagg tgcagtggga tgtggatggg attgaagggc
       61 tggacccacg cagctggtac ctcgtcaaca gcaaccacca gtcctgggtg gatattctgg
      121 tgctgcaata cctgctcaac cggcgcattc ccctgctcaa gtttttcatc aaacagcagc
      181 tgatctgggt gccggtcatg ggcctggcct ggtgggcgct ggactttccg ttcatgcgcc
      241 ggcacagcga ggaattcctg cgcaagcacc ccgagatgcg cggcaaagac caggaaacca
      301 cccgcaaagc ctgcgccaag ttcgccctga ttcccaccag cgtgatgaac ttcctggagg
      361 gcacgcggtt taccgttgcc aagcatgcca agcagcaatc accctacacc cacctgctca
      421 agcccaaggc tggcggcatg gcgctggccc tgaacgcgat gggcgacaaa tttcgcgcca
      481 ttctggacgt gaccatcgtg taccccgatg gggtgcccac cttcggagaa ttcctgagcg
      541 gcaagctgcg tcgtgtggtg gtgcgtgtgc gccgcctgcc tatccccgaa gaactgctgc
      601 acggtgacta cggacaggat gccgcatttc gcgaagcgtt ttccgcctgg gtgcaacaac
      661 tctggcgtga caaggatgcg cagatcagcg cactgcttct tgcctcccgg ccggccggca
      721 acaaaagtcc gtaatcccct gggtctcatc tgcggaaacg ctacttgccc agatatgctt
      781 cctcttccag cgcaagttcg gcatcacgct caaataacag gtcaataaaa cgctgtgccg
      841 ctacagacaa tgtgcgtcgg cgaagccgca ccactccaaa ctgccccccc acacccaaat
      901 tgataccagg aatggttatc agtttgccac tgcgcagttc ttcaagcacc atgaacgcat
      961 tcatcacggt cagcgcgtca ctgtggaaga taacgctctt cagcacagaa gaactgtcac
     1021 acgtaatgcg catcacaccc cgtttcagca ctacagcccg cagttcggct ggcaaatgcg
     1081 acaaaacctg ttcaatggca cccgacggca aattgggcgc ggccaatgga aatgcgaaca
     1141 catcgcgggc ggttggagcg gcgaacttgc tcagggggtg attggcacgg cacaccagca
     1201 aggacgggtg cttcagcaat ggctggactt ctatctcatc atccacaggt acatcactac
     1261 tattgatgat ggccagatcg atttcccggg tgttcaggcg cgctggcatt tctttccacg
     1321 gacaaatcac caggttcacg gtgaggtgcg gatattgcaa actcatggca cccaccaccg
     1381 caccagccag caccgctgcg ccccagggcc ccacaccaat cttgagttca ccactcgaca
     1441 gccccttcgc cagttcgatc tcacgttgca ggtcgcgtgc agccaaatcc aggtcacgtg
     1501 cgtttttgag caacagttgt ccaaaaatgg tgggctccac gccgccatgt gaacggtcaa
     1561 acaggatgac ctccagcgac tcttccagcg actgaatgct gcgactgagt gcggattggg
     1621 aaatgtgcat tgcctcggcc gcgcgggcaa agttgcgatg ctccgccaga cccaatgcat
     1681 gtcttagatg ccgtaattcg aacacgatgc actccgctca ttgagttgat gtaaacaatg
     1741 cattggacga attatgagtc gaactccaga atgcacctca tccaaaaaac aacaacggag
     1801 acaacaatgg aacgtcgcga actgtttagg cattcaatga tggcaatggg ggcaggactg
     1861 gcaggtctga cgcccggttt gagcctggcc caggaagaaa aaaaggtgca ccccgcgcaa
     1921 ctggacatgc tcaaggggca ggaaggtgtg gggaacccac gcgaggcaca tgcctatgcc
     1981 atcggcatgc aggcgtttga atacggtttc ccgctggtct acatggccag tctgagctac
     2041 accttcaaca aggatccaat ggggcagcag gtaccgctga atgcctttat cagccccaag
     2101 gactttgtcg cgacttcaaa cttccgcaac ggcggctcgt tcaacaccga cacggtctat
     2161 gccggtgcct tcattgattt gcgcaaaggc cccatggtgc tgaccaccca ggaccccgaa
     2221 ggccattact tctcggtgca gttgtcggac ctgtacacca ataactttgc ctacatcagc
     2281 aagcgttctg gtggacccat ttttggcaac ttcctgctgg tccctccggg ctggacgggc
     2341 aaaatcccca agggcaaatt cgaccgcgta ttgcagtgcg agcaaaagtg gctgtttgcc
     2401 ctggtgcgcc tgcgtatcga tcaggacgat gccgccgaag tgacctgggc caaggccaaa
     2461 ttcaagcagg cccagttcac gccgatggat gactttttgg caggccgcac gtttacgccg
     2521 aacgacaaca acgtgttcga cgtcacgcag ttcaagggca acccgctacg ccccttcgcc
     2581 atcttgaacc acatgctgac ggagaacccg ccgcccgcaa cggacgaaat cctgctgcag
     2641 ggcttcaaga cggtccacat cgggccaggc atggatcttt ccaaaaacga ccccgacacc
     2701 ctgcgcggcc tggcccgtgc cgcccgcgac ggcctgcccg cgctgcgctc gcacgtagag
     2761 cacccgtggg cgcggtcggt caatggctgg ttttacttcc ccaccaatat cgggcaggcc
     2821 aagaccaccg actacatcat gcgttcggca tggcagtcgc tgtggggcat cgtggcgcac
     2881 cctccgcgtg aatgcaccta cctgtgggct tcgcaggacc aaaatggcga gcggctcaat
     2941 agcaacggca agtatgagct gtatatgcgc aaagaccagt ttccaaaagt cgaggcgttc
     3001 tggtccttca ccatgtacca ggatttcaac ttggtggtga acgacgccaa ccgctgggcg
     3061 attcgtgaaa acatgaaggg cctcaagttc gatgcagatg gtggcctgcg tatttatatc
     3121 cagaccgagc gcccgtccga ggacaaggtc agcaactggt tgcctgcacc caagagcggc
     3181 aatttcaaca tcacgatgcg caactacatg ccatcacagg agattgtgga acagcggtac
     3241 gacccaccgg tcatgaagcg cgtctcctga tttgacggag cccgcccagc gcttgaaaaa
     3301 gcgggctggg ctcctgctgg gttcttttgc ggtacagact tgaggagtgc aagtttttga
     3361 gtcaaatctc accccagccc tctatttata tgcataagca gctataacaa taatagcaat
     3421 ttcccgagga gaaacccatg aagaagatca gttacatgct tttggcggcg tgcactgtgc
     3481 tggctgccgg ttgcagcaaa aacaccggcg tcggcggtgc agcgggggaa gccacacctg
     3541 caactatcca agccaatacc cagttggcca aggacctgaa gctgggtgac caacaagact
     3601 ttgaagacgc caagcgcggc tttattgccc gacccaatgg caaagtgctg tccgcagacg
     3661 gaaccgtact gcacgactac gacgcatacc agttcattga agggcaagcc ccggacacgg
     3721 tcaatccgag cttatggcgt cacgcgctgc tgaatgcaca gatgggtctt ttcaaagtca
     3781 cggacggcgt ctaccaattg cgtggttttg acattgccaa catcaccctg attgagggca
     3841 aatctggctg gatcgtggtg gacgcactca ccagtcgcga gagtgccagc gccgccatgg
     3901 cttttgcgcg caaacacttg agcgacaagc cggtcacggc gcttattttt acgcacagcc
     3961 atgccgatca ttttgggggg gcgctaggca tcattacgcc ggaagaagtg gccaaacgca
     4021 aagttccggt gctggcatcg
//
LOCUS       DAOSJM010000223         4036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19633_length_4036_cov_3.868877, whole genome shotgun sequence.
ACCESSION   DAOSJM010000223 DAOSJM010000000
VERSION     DAOSJM010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19633_length_4036_cov_3.868877"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PKX82_08580"
     CDS             <1..620
                     /locus_tag="PKX82_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOF52349.1"
                     /translation="VPLIKGYSTEMSLEVTSLAVQVHGGMGFIEETGAAQYYRDAKIL
                     TIYEGTTAIQANDLVGRKTSRDGGQTAKGIAGQIETTEAALIQSGTADALAMAKRLKA
                     ARLAFIDVVDFVAGNTKASPNAVFAGSVPYLMLAGNLMAGWQLARALLVAQEQLAAGT
                     DATFMQAKITTARFYADHILSKVPGMRDSIVDGADSVTAMPLDAF"
     gene            721..1686
                     /locus_tag="PKX82_08585"
     CDS             721..1686
                     /locus_tag="PKX82_08585"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HOF52350.1"
                     /translation="MSKLPAPLDRLPFPVIGSPLFIISNPKLVIAQCIAGVVGSMPAL
                     NARPAEQLEEWLIEITEALAAYNKAHPDKPAAPFAINQIVHKSNDRLEHDMAMCVKYK
                     VPIIITSLGAREEINAAAHSYGGVVLHDVINNKHAHKAIEKGADGLIAVSAGAGGHAG
                     VKSPFALVQELRQWFDGPLALSGSISTGSAILAAQAMGADFAYMGSAFIATEEARASD
                     AYKQAIVDNDSDEIVYSNLFTGVHGNYLAPSIRNAGLDPANLPESDPSKMNFGGASAK
                     AWKDIWGCGQGIGAISKVQSTADYVAQLKREYAEARSQLLARNYA"
     gene            complement(1693..>4036)
                     /locus_tag="PKX82_08590"
     CDS             complement(1693..>4036)
                     /locus_tag="PKX82_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOF52351.1"
                     /translation="EQHTMRATPIANAPATTPEQRAKALRLVRTLMGKTLGYFEDRED
                     AQINQIRARLFELADSKLPLLQSQNLRTAAMLLDRQAVKFKRLYRDAIQQCLQEEIQG
                     IWPEMKQQGSQSSEHPASHALNGMTLSLIDVDEVHRILLLDQLAEKFNTRYEPVLTPL
                     TQKLCALLGQETPSLSSNPFRPGVLLLAFQRAWEKGAFDPATTEHLIQSLDPAHTMDL
                     APLYTELNSTLAQAGIQAHTVHRIRKSVSSSYAPLTGSAPLGEANSSGHGPLHSQPAP
                     LTGNAGLSPELPRSAWGALAPAGQHIATQVKAFLHRLGVGAASDSQVVQSHAAAQQTP
                     DPALLNYLGEMQAGSEAQSSRFAFLQPQNAEHGHHNVLRQIRDQEEIRQAPELDRTTV
                     DALAEVFDYVFADQAIPMQMKVVIGRLQIPVLRAAMIDRDFFLRTDHPARQLVDTLAS
                     SSVTWTPDKGEQDPLYMRIEQTVQRVLTEFEDDLTLFSILLAEFTEFLFESEQQVQLQ
                     ITPVVTQESDKEALDAALAQADEAVHARISALPDTLPLAPFLTPFLTTQWREVLARAW
                     IQEAQEPGRWESALRTMDRLIWSTQPKTTTEDRRDLVALLPELVRTLNAELDAIEWSG
                     EPRATFTRRLISTHMLAIRMKAQPPEDTQNAALEARAGQEALQALDQRRSTQLSAHNL
                     SEMDEFDASAQLLTRGVWFEASLNGAAAYRCRLSWVSPMRTRFLFTNRDGFDAFVRNE
                     NEVATMLRQGQLTQLEQAPIVGRALDHLMAANDSELTLAA"
BASE COUNT          731 a         1221 c         1293 g          791 t
ORIGIN      
        1 tggtgccgct gatcaagggc tacagcaccg agatgagcct ggaagtgacc tcgctggcag
       61 tgcaggtgca cggtggcatg ggcttcatcg aggaaaccgg cgcggcccag tactaccgcg
      121 acgccaagat cctgaccatc tacgaaggca ccaccgccat ccaggccaac gacctggtcg
      181 gtcgcaagac ctcgcgcgat ggcggccaga ccgcgaaagg cattgccgga cagatcgaga
      241 ccaccgaggc cgcgctcatc cagagcggca ccgccgatgc gctggccatg gccaagcgcc
      301 tgaaggctgc acgcctggcg tttatcgacg tggtcgattt tgtggccggc aataccaagg
      361 ccagcccgaa cgccgtgttt gccggcagcg tgccctacct gatgctggct ggcaacctga
      421 tggcgggctg gcagctggcg cgcgcactgc tggtagccca ggaacaactg gcggccggga
      481 ccgacgctac cttcatgcag gccaagatca ccacggcccg cttctatgcg gaccacatcc
      541 tgagcaaggt gcctggtatg cgagacagca tcgtcgacgg tgcggacagc gtgaccgcca
      601 tgccgctgga tgcgttctga ttacttcagc cttaatttgc tattgaattt gtagctaccc
      661 gcgcaataga tgcgggggct acagcccttt ttttctataa atccccattg aggagacacc
      721 atgtccaaac ttcccgcgcc gctggaccgc ttgccttttc ccgtgatcgg ctcgcccctg
      781 ttcatcatca gcaaccccaa gctggttatc gcccagtgca ttgccggggt ggtgggttcc
      841 atgccagcgc tcaatgcgcg tccggccgag cagctcgaag agtggctgat tgaaattacc
      901 gaggcactgg cggcttacaa caaagcccat cctgacaagc cagcagcgcc gtttgccatc
      961 aaccagatcg tgcacaagag caatgaccgg ctggaacacg acatggccat gtgcgtcaag
     1021 tacaaggtgc caatcatcat cacctcgctg ggcgcccgtg aagagatcaa tgcggccgca
     1081 cacagctacg gcggcgtggt gttgcacgat gtcatcaaca acaagcacgc ccacaaggcg
     1141 atcgagaagg gcgccgatgg cctgatcgcg gtgtcagcag gtgcaggtgg ccacgcgggc
     1201 gtgaagagcc cgtttgccct ggtgcaggaa ctgcgccagt ggttcgatgg cccgctggca
     1261 ctcagcggct ccatttccac cggttcggcc attctcgctg cgcaggccat gggcgcagac
     1321 ttcgcctaca tgggttcagc tttcatcgcc accgaagaag cacgtgccag tgatgcctac
     1381 aagcaggcca tcgtggacaa cgactctgat gaaatcgttt acagcaacct gttcaccggt
     1441 gtgcatggca actacctcgc acccagcatc cgcaatgcgg gtctcgaccc ggccaatttg
     1501 cccgaaagtg accccagcaa gatgaatttt ggtggtgctt cggccaaggc ctggaaagac
     1561 atctggggct gtggtcaggg cattggcgcc atttccaagg tgcagtccac cgccgactat
     1621 gtggcgcagc tcaagcgcga atatgcagag gcccgttcgc agctgctggc ccgcaactac
     1681 gcctgaggca actcaggctg ccagcgtcag ttcggagtcg ttggcggcca tcaggtggtc
     1741 cagggcgcgg cccacaatgg gggcctgctc caactgcgtg agctggccct ggcgcagcat
     1801 ggtggccact tcattttcgt tgcgcacgaa tgcgtcgaaa ccgtcccggt tggtgaacaa
     1861 aaaacgcgtg cgcatggggc tgacccagct caaacggcag cggtaggccg ctgctccgtt
     1921 caggctggct tcgaaccata cgccccgcgt cagcagttgg gcactggcat cgaactcgtc
     1981 catctctgac aggttgtgcg cactgagttg ggtggaccgg cgctggtcga gcgcctgtaa
     2041 tgcctcctgc ccggcgcgcg cttcgagtgc cgcattctgg gtgtcctcgg gcggctgcgc
     2101 tttcatgcgg atggccagca tgtgggtgct gatcaaccgg cgtgtgaaag tggcacgggg
     2161 ttcgccgctc cattcgatcg catccagctc ggcattgagc gtacgcacca gttcgggaag
     2221 aagtgccacc agatcccggc ggtcttcggt cgtggtcttg ggctgggtac tccagatcag
     2281 ccggtccatc gtccgcagcg cggactccca gcggccaggt tcttgcgctt cctgtatcca
     2341 cgccctggcc aggacctcgc gccactgcgt ggtcagaaac ggggtgagaa atggcgccag
     2401 cggcagggtg tctggcaggg cgctgatgcg cgcatgcacg gcctcgtcgg cctgcgccag
     2461 cgccgcgtcc agtgcttcct tgtcactttc ctgcgtgacc accggggtga tctgcaactg
     2521 gacctgctgt tcactctcga agaggaactc ggtgaactct gccagcagga tgctgaacag
     2581 cgtgaggtcg tcctcgaact ccgtcaggac gcgctggact gtctgctcga tccgcatata
     2641 cagcgggtcc tgctcgccct tgtccggcgt ccaggtgacc gacgaggacg ccagcgtgtc
     2701 cacaagctgg cgggcagggt ggtcggtgcg caggaaaaag tcgcggtcga tcatggctgc
     2761 gcgcagtacg ggaatctgca ggcgcccaat gaccactttc atctgcatcg ggatggcctg
     2821 gtcggcaaac acgtagtcga aaacctcggc cagtgcgtcg accgtggtgc ggtccagttc
     2881 cggtgcctgg cgaatctctt cctggtcccg aatctggcgc agcacattgt ggtggccgtg
     2941 ctccgcgttc tgtggttgca aaaaagcgaa acgcgaggac tgcgcttctg atccggcctg
     3001 catttctccc aggtagttca gcagtgctgg gtcgggtgtt tgctgggccg cggcatggct
     3061 ttgcacgacc tgcgagtccg aagctgcgcc aacgcccagc cgatgcagga aggccttgac
     3121 ctgggtcgca atatgctggc ctgcaggtgc cagtgccccc caggcacttc tgggaagctc
     3181 gggcgacaga cccgcattgc cggtcagtgg cgctggctgg gaatgcaggg ggccatgccc
     3241 gctgctattc gcttcgccca gcggtgccga tccggtcaat ggtgcatacg aggacgagac
     3301 ggatttgcgg atgcggtgca cggtgtgtgc ctggatcccg gcttgcgcca gggtggaatt
     3361 cagttcggtg tacagcggtg ccagatccat ggtgtgcgct ggatcgagcg actggatgag
     3421 gtgctcggtg gtcgccggat cgaacgctcc tttttcccag gcccgctgaa aggccagcag
     3481 caacacacca gggcggaatg ggttgctcga cagcgaaggt gtttcctgtc ccagcagggc
     3541 acacagcttc tgggtcagcg gggtgagtac cggttcatag cgggtattga acttttctgc
     3601 cagctggtcg agcagcagga tgcgatggac ttcgtccaca tcgatcagac tcaaggtcat
     3661 gccgttgagg gcatggctgg cagggtgttc ggaggactgg ctaccctgct gtttcatctc
     3721 gggccagatg ccctgaattt cttcctggag gcattgctgg atcgcgtcgc ggtacagacg
     3781 cttgaatttc acggcctgcc ggtccagcag catggcggcg gtgcgcagat tctgggactg
     3841 caacaggggc agtttgctat cggccaactc gaacagacgg gcccggatct ggttgatctg
     3901 ggcgtcttcc cgatcctcga agtagcccag tgtcttgccc atgagggtgc gcaccagccg
     3961 cagtgccttc gcacgctgtt cgggcgtggt ggcgggggca ttggcaatgg gcgtggcacg
     4021 catggtgtgc tgttcg
//
LOCUS       DAOSJM010000224         3986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_19959_length_3986_cov_4.327398, whole genome shotgun sequence.
ACCESSION   DAOSJM010000224 DAOSJM010000000
VERSION     DAOSJM010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19959_length_3986_cov_4.327398"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..427)
                     /locus_tag="PKX82_08595"
     CDS             complement(47..427)
                     /locus_tag="PKX82_08595"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HOF52352.1"
                     /translation="MKQYRVFHHPSGRAEAVKQGWSWPASLFSVFWAPFKGLWVLGVG
                     GLLALAGLGYLAASFTDANTAASVMNLVLTAAGILFGVKGNAWREAGLIKRGCRLVGT
                     VMAEDAKGATANANANANIVAKKT"
     gene            970..1878
                     /locus_tag="PKX82_08600"
     CDS             970..1878
                     /locus_tag="PKX82_08600"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF52353.1"
                     /translation="MDLNLFRVFDAIYSHGGISGAARALHLTQPAISHALRRLREVFN
                     DPLFQRSGNRMVPTDLTRTVIADIRGHLQGLFGSIHATGEIIPAELQTEFRLAMPDVA
                     EATALPNLIRRLHQIAPDVRVVCREVARENFERELAKGSLDFVFDRRFAASPQLQCVH
                     MGQQEMAIVAAEQRFPEQQARISSKDYLRARHAVVTHLEGHDPIDSILAESGASRRVV
                     LRCAHYLSACRVVAQSDLLLTMPRRYAEELATLMPLRVLDMPIPIAPVQVYLYWHRVR
                     DDDRVHRWMREEMIALSEKGSVRAKS"
     gene            2134..3582
                     /locus_tag="PKX82_08605"
     CDS             2134..3582
                     /locus_tag="PKX82_08605"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HOF52354.1"
                     /translation="MNKKIWIALFVLIIGFLAAGAFVWKRYIRAPWDDEYAYTKGLQA
                     VVYAFPYVLNSSVRWAWSQPQKEGEPVVAPTDAINHFFHSPRLTGPQYRDGGTPNNDT
                     AYSTTWAYVDKEPLVISVPEMGNTPGTSKPRYYSLQLAAFDSDNFAYIGTRTTGNGAG
                     HYAITPKGWQGRLPSNVKLLAEAPTPWFLILGRTLVTSNEDFPAVAALMHQFDLRTLG
                     DFENNVQRRPPAPALTPVPHYKKEQLALVSNFWSIANSAMTANPPGQEDARLMRFFRD
                     IEVGPGRDLSGLGEGMRRGLDRAVLRGLTLLPEVNQTNFGTKLVNGWKYPPMDYGRAG
                     VNGAWLTRASLQSLGGIVANDPEEAVYIVAHADSGGRLLNGKSHYRIHFAKGQIPPVK
                     AFWSMSLYDNTNNLVDNSLNRYSLGDRSPGLVFNPDGSLTLYVGHELPEGAAKSNWLP
                     APADEFYLVLRAYLPGKPIVDQIWEPPAVVRQ"
     gene            complement(3793..>3986)
                     /locus_tag="PKX82_08610"
     CDS             complement(3793..>3986)
                     /locus_tag="PKX82_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOF52355.1"
                     /translation="LQAWFAAGASTASVVWFSLLGVGARWLAPWFARPTAWRVLDGLI
                     GVTMAVLAVAMARHAWGGI"
BASE COUNT          807 a         1190 c         1164 g          825 t
ORIGIN      
        1 gaacctggcg cgtcggacgg ttctgtcccc cagcagacgt cgtcggtcag gtctttttcg
       61 caacgatatt ggcgttcgcg ttcgcgttcg cggtcgcgcc cttggcgtct tcggccataa
      121 cggtccccac cagcctgcag ccccgtttga tcaggcccgc ctcgcgccag gcgttgccct
      181 tgacgccgaa gagaatcccc gctgcggtca acaccagatt catgacggat gcagccgtgt
      241 tggcgtccgt gaaggaagcc gccaggtagc ccaggcccgc caaagcgagc aggccgccca
      301 cgcccagcac ccacaagccc ttgaatggcg cccagaagac gctgaacaga ctcgccggcc
      361 aagaccatcc ctgtttgacg gcctcggccc ttcccgaggg gtgatgaaag acacggtact
      421 gcttcatggt ggccctttgt ggcagtcctg ccagctgccc gatggtacgg cggctcgccg
      481 gaacgcctgg aaagctccct cagacagtgg acgaggcgcc agaggtgatc atttgggcgt
      541 tctgggtagt gctccgcggg gaccaccaaa aagttcaccg atgatcatct gtggtgtgcc
      601 ttgggcagga ctttggcatt gaaagaaggg agcggctttc gctctggagc gattcgacgg
      661 tgaccgccac gggccgtcag cttcaggctg caagccggtg tagcgtttgc gaacagccca
      721 cgccaaacgg gccactgacg atcaagggaa gatgagcaaa atcaaaacag aacagaaaca
      781 agatcatttc tgttgcatac agaatgacgg caacaatgat cagcgaagtt tgcagggaat
      841 tccggagcgc tcgaacccca ttgttgaact atccaatttt ccgaccgacg tcacttcacg
      901 cttttgttca tgccacggcg cactccatcc ccaatacaaa tcggatccag ctacgcattc
      961 gacccgaggt tggatctgaa cctgtttcgt gtgttcgatg ccatctattc acatggcggt
     1021 atctccggcg cggcacgggc attgcacctg acgcaacctg cgatcagcca tgcgctgcgg
     1081 cgcttgcgcg aagtgttcaa cgatccactg tttcagcgca gcggcaatcg catggtgcca
     1141 acggacctca cgcgcacggt gattgccgat atacgcgggc atttgcaggg gctcttcggt
     1201 tctattcacg cgaccgggga gatcattccg gctgagttac agacggagtt tcgactggcc
     1261 atgcccgatg ttgcggaggc caccgccttg cccaatctga ttcgccgtct gcaccagatc
     1321 gcaccggacg ttcgcgtcgt gtgtcgggaa gtggcgcgtg agaacttcga gcgcgaactg
     1381 gccaagggca gcctcgattt tgtctttgac agaaggtttg ccgcttcgcc gcaactgcaa
     1441 tgcgtgcaca tggggcagca ggaaatggcc atcgtggcag ccgaacagcg tttccccgaa
     1501 caacaggcac gcatcagctc gaaggactat ttgcgtgcac gccatgccgt ggtgacgcat
     1561 ttggagggac acgaccccat agacagcatc ctggcggaaa gcggggccag ccgccgtgtt
     1621 gtgctgcgct gtgcccacta cctttccgct tgtcgcgttg tggcgcaaag tgatttgctg
     1681 ttgaccatgc cacgccgtta tgccgaagaa ctggcgacgc tgatgcccct gcgggtgttg
     1741 gatatgccca ttcccatcgc gccggtgcag gtgtatctgt actggcaccg tgtgcgggat
     1801 gatgaccgcg tacaccgctg gatgcgggaa gagatgattg ccttgtcaga aaaaggcagc
     1861 gttcgcgcaa agtcctgagt cgagcacacc tgcgggctgg gggatggcgc acttaccagt
     1921 gccaatggaa gagcgatcga tgcgatgaca ccgcgtgtcc atgaatttcg ttcataactt
     1981 gacatcaata catgtcactt gcctgcatga gtggccagcg cctagcatgc ggttcatgtt
     2041 gcaagcgcgt tgtctctttc gcacattcac gccgcagatc aatgggctgc ataccgcttc
     2101 ttcatttcat tgccaggggt accttcgatg ggtttgaaca agaaaatctg gatcgcactg
     2161 tttgttttga tcattgggtt cctggcagcc ggcgcgtttg tctggaaacg ctatattcgc
     2221 gcgccctggg atgacgagta cgcctatacc aagggattgc aggccgttgt ttatgcattt
     2281 ccctacgtgt tgaacagttc ggtgcgctgg gcctggtcac aaccgcagaa agagggtgaa
     2341 cctgtcgttg caccgaccga tgccatcaac cactttttcc attcgcctcg tttgaccggt
     2401 ccgcaatacc gtgatggcgg tacgcccaac aatgacacgg cctattcaac cacctgggcg
     2461 tacgttgaca aggagccgct ggtcatcagc gttcctgaga tgggaaatac tccaggaacc
     2521 agcaagccgc gctattacag cctgcaactg gccgcctttg attccgacaa ctttgcctat
     2581 atcggcaccc gcaccacggg caatggcgca gggcactatg ccattactcc caagggttgg
     2641 cagggacgct tgccatccaa tgtgaagctc ctggccgaag cgccgacacc ctggttcctc
     2701 atattgggcc gcaccttggt caccagcaat gaagatttcc cggctgtggc agcgctgatg
     2761 catcaatttg acctgcgcac cctgggtgat ttcgagaaca acgtgcagcg tcgtccacct
     2821 gcacctgcgc tgacaccggt gccgcattac aagaaagaac aactcgcgct ggtcagcaat
     2881 ttctggtcca ttgcaaatag cgcgatgacg gcgaatcctc cggggcaaga ggatgcacga
     2941 ttgatgcgtt tctttcgcga catcgaagtc ggacccgggc gcgatctttc aggattgggc
     3001 gaaggcatga gacgtggact ggatcgcgcc gtgctgcgcg ggctgaccct gctgccggag
     3061 gtcaaccaga ccaattttgg aaccaagctg gtcaacggat ggaagtaccc tccaatggat
     3121 tacggccgcg ccggtgtcaa tggcgcctgg ctcacgcgcg cctcactgca atcgcttggc
     3181 ggtatcgtgg ccaatgaccc ggaagaggct gtgtacatcg tggcccatgc cgattccggc
     3241 gggcggcttc tcaatggcaa atcgcattac cgcattcact ttgccaaagg ccagattcct
     3301 ccggtgaagg ccttctggtc gatgagcctg tatgacaaca ccaataacct ggtggacaac
     3361 tcgctgaacc gctattccct gggtgatcgc agtcctggcc tggtattcaa ccccgatggc
     3421 agtctgacgc tctacgtggg tcatgagctg cctgaagggg ctgcgaaaag caattggctt
     3481 ccagccccag cggacgagtt ctacctggtc ctgcgtgcgt acctgccagg caaaccgatt
     3541 gttgaccaga tctgggaacc gcctgcggtg gtgcggcaat agctatctgg cgaagcagcg
     3601 tctgcgttgt ttgtaagcct tgccggggcg ccgacacgtc gcgcaacgat gggcgcaggc
     3661 tggaccaaga attcagagat ttggtttctc ttttacggac cttgatgtcg ggcagcaagt
     3721 gctgccttgc ggtccatcga atcgatgctg tggccagccg caatttggtc cctgtcggga
     3781 ttcgacaagc cttcagatcc cgccccaagc atggcgcgcc atcgccaccg ccagcacggc
     3841 catcgtcacg ccgatcagtc cgtccagcac gcgccaggca gtgggccgtg cgaaccaggg
     3901 cgccagccag cgcgcgccca cacccagcag gctgaaccag accacgctgg cggtgctggc
     3961 gccggcggca aaccaggcct gcagcg
//
LOCUS       DAOSJM010000225         3909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_20442_length_3909_cov_3.941879, whole genome shotgun sequence.
ACCESSION   DAOSJM010000225 DAOSJM010000000
VERSION     DAOSJM010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20442_length_3909_cov_3.941879"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PKX82_08615"
     CDS             complement(<1..412)
                     /locus_tag="PKX82_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HOF52356.1"
                     /translation="MMKLWTLGLALVLALSGINAEAAKRLGGGKSVGKQSSNVTQQAA
                     PAAPGAPAQSAAAAPKPAPAAAPAAAPKRPWGAMLGGLAAGLGLAWLANSLGMGEAFG
                     NILMFGLLALGVMMLVGWFMRNRRPAAPLQNAGPY"
     gene            complement(434..1165)
                     /gene="ubiE"
                     /locus_tag="PKX82_08620"
     CDS             complement(434..1165)
                     /gene="ubiE"
                     /locus_tag="PKX82_08620"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOF52357.1"
                     /translation="MSTTHFGFKSVDETEKARHVRTVFDSVAPKYDLMNDLMSAGLHR
                     AWKAYTVMVANLKEGDRALDIAGGTGDLALAFSKKVGKSGQVVHTDINEAMLSTGRNR
                     LLDLGVALPTMVCDAEKLPFPDNYFDVVSVAFGLRNMTHKDVALAEMNRVLKPRGKLL
                     VLEFSKVAAPLEKVYDWYSFKVLPRLGKLVAGDDSSYRYLAESIRMHPGQQELKAMMH
                     KGGFGHVDYHNLTGGIAALHVGIKC"
     gene            complement(1246..1659)
                     /locus_tag="PKX82_08625"
     CDS             complement(1246..1659)
                     /locus_tag="PKX82_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOF52358.1"
                     /translation="MAGLTTGAPAPTNITVHAQSRALEISFSDGATFRIPFELMRVYS
                     PSAEVQGHGPGQETLQTGKRLVDLTGLEPVGNYAVQPGFSDGHDSGIFSWDYLYFLGS
                     QQDQLWQDYEAKLSAAGVDRDTPMPEKSGHSCASH"
     gene            complement(1678..3909)
                     /locus_tag="PKX82_08630"
                     /pseudo
     CDS             complement(1678..3909)
                     /locus_tag="PKX82_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837116.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
BASE COUNT          705 a         1248 c         1190 g          766 t
ORIGIN      
        1 catagggccc cgcgttttgc aagggggcag caggacgacg attacgcatg aaccagccca
       61 ccagcatcat gacgcccaaa gccagcaagc cgaacatcag gatattgccg aatgcctccc
      121 ccatacccag ggagttggcc agccaggcca gccccagacc cgcggccaga ccacccagca
      181 tggcgcccca cgggcgcttg ggggcagcgg cgggtgcggc tgccggagca ggcttgggag
      241 cggctgccgc gctttgcgcc ggagcaccgg gcgctgccgg tgccgcctgc tgcgtcacat
      301 tggagctttg tttgcccact gacttgccgc cacccagccg tttggccgcc tctgcgttga
      361 tgcccgacag agccagaacc aacgccaatc ccaatgtcca cagtttcatg atgtctccga
      421 aaaacgaata atctcagcac ttgattccaa catgcaatgc ggcaatccca ccagtgaggt
      481 tgtgatagtc cacatgaccg aatccacctt tgtgcatcat ggcctttagt tcttgctgac
      541 ccgggtgcat gcgaatcgac tcggccaggt accggtagct ggagtcgtcg cccgccacca
      601 gcttgcccag ccgcggcagc accttgaagg aataccagtc gtacaccttc tccagcggag
      661 cggccacctt gctgaactcc agcaccagca gcttgccgcg cggtttgagt acccggttca
      721 tctcggccag ggccacgtcc ttgtgcgtca tgttgcgcag gccgaaggcc acgctgacca
      781 cgtcgaagta attgtccggg aaagggagtt tctccgcatc acacaccatc gtgggcaagg
      841 ccacgcccaa gtccagcagg cgattgcgcc cggtggaaag catggcttcg ttgatatcgg
      901 tgtgcaccac ctgaccggat ttgccgacct ttttggagaa agccagcgcc aggtcaccgg
      961 tgccaccggc gatatcgagt gcgcggtcgc cttccttcag gttggccacc atcacggtgt
     1021 aggccttcca ggcgcggtgc aatcccgccg acatcaggtc gttcatcagg tcgtacttgg
     1081 gcgcgaccga gtcaaacacg gtgcgcacat ggcgcgcctt ctcggtctca tcaaccgact
     1141 tgaatccaaa atgggtggtg ctcatggttt gattctaagc aaaatcggca accagccccc
     1201 agaaaatatg cgcaacacgc tatcaaaaag atagcgactg caagatcaat ggctggcgca
     1261 gctgtgaccg gatttttccg gcatgggcgt atcccggtcc acgccggccg cagacagttt
     1321 ggcttcgtag tcctgccaga gctggtcctg ctgcgagccc agaaagtaca ggtaatccca
     1381 ggaaaaaata cccgaatcgt gtccgtccga aaatccgggc tgcacggcat agttccccac
     1441 aggctccagc ccggtgaggt cgaccagacg cttgccggtt tgcagcgttt cctggcctgg
     1501 tccgtggccc tgcacttcgg cagacggcga gtacacccgc atcaactcga acgggatacg
     1561 aaatgtcgcc ccatcggaaa agctgatctc cagcgcgcgg gactgcgcat gcactgtgat
     1621 gttcgtcggt gcaggagcac ctgtagtcaa gcctgccatg cgtttgtcct ctgttaccta
     1681 gaccagtacc cgctctatgc cacccgcatt ggcggtctgt acatactccg gcagccattg
     1741 cttgcccaga atctggttcg ccatctcgac cacgatgtag tcggcttcca gcagcccatt
     1801 gttcaggtcg ttgccatagc gggtcaaccc ctgcaggcag ctgggacagc tagtcagaat
     1861 cttgacattg ccctgttctg ctaccgcacc actggcgcgc agttcggctt cgcccttgcg
     1921 gatttcctct tccttgcgaa agcggacctg ggtcgaaata tcggggcggg tcacacccag
     1981 cgtgccagac tcgccacagc agcgttcgct cttgagcacc ttgtcgccga caagggcctt
     2041 gacggtcttc atcgaatccc ccagcttcat ggggttgtga cagggctcgt ggtacaggaa
     2101 accaccctgg cccgcaggca aagtgatacc tttttccagc aggaactcgt ggatgtcaat
     2161 gatgcggctg ccggggaaga tctcttcgaa cttgtaaccc tgcaactggt cgtagcaggt
     2221 accacagctc accaccaccg tcttgatatc caggtagttc agcgtgttgg ctacgcggtg
     2281 gaacagcacc cggttgtcgg tgatcatctt ctcggccttg tcaaactggc cgctgccacg
     2341 ctgcgggtag ccgcagcaca ggtagcccgg cggcagcacg gtctgcacgc cggcatgcca
     2401 cagcatggcc tgcgtggcca gcccgacctg gctgaacaga cgctccgatc cgcagccggg
     2461 gaaatagaac acggcttccg tctcggcggt cgtggtcttc gggttgcgga tgatcggtac
     2521 gtagtccggg ttctcaatgt ccagcaaggc acgcgcggtc ttcttgggca ggccaccggg
     2581 cattttcttg ttgatgaagt ggatcacctg ctccttgatc ggcgcagtgc ccaccgtggc
     2641 cggtggcttg ttcgtctgtt tccgcgcagc caggcgcagc acctggttgg ccagacgctg
     2701 ggccttgaag cccacgccca ccatggcggt acgcatcagc ttgatggtct cgggattggt
     2761 ggcgttgagg aagaacatgg ccgcggcatt gcccggccgg aacgacttct gccccatctt
     2821 gcgcagcagg ttgcgcatgt tcatggtcac atcgccgaaa tcgatcttca ccgggcaggg
     2881 cgacaggcac ttgtggcaga ccgtgcagtg gtcggccacg tcctcgaact cttcccagtg
     2941 cttgaggctg acgccgcggc gggtctgctc ttcgtacagg aaggcctcga tcagcagcga
     3001 ggtggccagg atcttgttgc gcgggctgta cagcaggttg gcgcgcggca cgtgggtggc
     3061 gcacaccggt ttgcacttgc cgcagcgcag gcagtccttg atgctgtcgg cgatggcgcc
     3121 gatgtcgctc tgctgcatga tcagcgactc gtgccccatc agcccgaagc tgggcgtgta
     3181 ggcgtggctc aagtcggcgg cgtgcacttg cgcgtcggca ctgcccaggc cggccagggc
     3241 cgcgccacgc agcagcttgc ccttgttgaa gcggccttcg gggtcgatgc gggccttgta
     3301 gtcggtgaag gccgccatct cggcgtcgct caggaactcg agcttggtga tgccgatgcc
     3361 atgctcaccc gagatcacgc cgcccaggct gcgcgccagc accatgatgc gggccaccgc
     3421 ctcgtgggcc gtctgcagca tctcgtagtt gtcgctgttg acggggatgt tggtgtgcac
     3481 attgccatca ccggcatgca tgtgcagggc cacccaggtg cggccgcgca gcacctcggc
     3541 gtggatgcgc ttgaactcgg ccacgatggg cgcaaaggcc gcgccggcaa acagcgattc
     3601 aaagggcttg aggacctgca gcttccagct ggcgcgcagg ctgtggtcct gcagctggtc
     3661 gaagtagctg cggccgcctt cgccaccatc gggcgcgggc accgcgtcca gccgcatcag
     3721 ccagttcagc cattggccgc gtacctcgcg cagcgcggcc agggcctgct gcacgcggtc
     3781 ttccaggagc tcggcgctgg ggatctcgtt ggcgtcgtcg gtcttgccca gcggcaggct
     3841 gccggtggcc aagaaggcct ccagccggtc cagcaactgc agcttgttgc gcaggctcag
     3901 ctagatgtt
//
LOCUS       DAOSJM010000226         3901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_20494_length_3901_cov_4.747270, whole genome shotgun sequence.
ACCESSION   DAOSJM010000226 DAOSJM010000000
VERSION     DAOSJM010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20494_length_3901_cov_4.747270"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1709
                     /locus_tag="PKX82_08635"
     CDS             <1..1709
                     /locus_tag="PKX82_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52359.1"
                     /translation="AGSMSWAPQALPDRWRDTRPNTDGSAWYRMQFERDALGQALQAV
                     FIPHASVNAEVYLNGVLLGTGGSFEEPLSRTWNRPRMYLIAPEVLQTGRNTLDVRLKA
                     HLYTQASLYPPRIGPEKEIRDLYEQAYFLRITLNQIASILIAVVGLLMLNMWWRRRQD
                     TAYGYFAASALVWAVQSTNLYVLDPPLDTAHWEILVNASFQIFSALLLISMLRYVRAE
                     WKPLNLLLWSSLLASPLAMVLAPPESFLRVTAALHMCTLIGSVGSLVLLFRSAWRDRN
                     KDARLLLATMGLIVLFAAHDWLLHSQHLWFSGALKWLPGELYLLQYSAPVVFLAIGWI
                     MTVRFIQVLNEFEALSVELDQRVQAKHAQLEQSFERLRALERERAVQDERERIHSDLH
                     DDVGAKLLSLVYRAGSPEAADLARSALQDLRDVVSRVGSGSFQLEDVLADIRAECEQR
                     LSGASIALEWHQTEGIAPIELTQPHALNLGRIVRETISNAIRHAQASAVTVHITPDGE
                     GLHIEICDNGIGGAVDGHVPGRGLRNMQARAQLIGAQLERFSVLPQGCGVRLTIPSFT
                     GT"
     gene            1917..3260
                     /gene="gdhA"
                     /locus_tag="PKX82_08640"
     CDS             1917..3260
                     /gene="gdhA"
                     /locus_tag="PKX82_08640"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOF52360.1"
                     /translation="MKYPSVRAFLQHVEQRNPGQPEFLQAVTEVMESLWPFIEKHPRY
                     AEQGLLDRLVEPERIVMFRVSWVDDHGTVQVNRGYRIQHSMAIGPYKGGLRFHPSVTL
                     SVLKFLAFEQTFKNALTTLPMGGGKGGSDFDPKGKSPAEVMRFCQAFASELFRHIGAD
                     TDVPAGDIGVGGREVGYIAGMYKKLSNRADCVFTGKGLSFGGSLIRPEATGYGTVYFA
                     DEMLKTRGKSFEGLRVSVSGSGNVAQYAVEKAMQLGAKVITVSDSSGTIIDEDGFTPA
                     KLAILMEVKNHLYGRVSDYAARTGVKFEAGVRPWHVKVDVALPCATQNELDEKDAKTL
                     IRNGVLCVAEGANMPSTIEAAKAFEAAGVLYAPGKASNAGGVATSGLEMSQNAMRLSW
                     TRDEVDARLLQIMQGIHAACLQYGKRADGSVSYIDGANVAGFVKVADAMLAQGVI"
     gene            3348..>3901
                     /locus_tag="PKX82_08645"
     CDS             3348..>3901
                     /locus_tag="PKX82_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52361.1"
                     /translation="MIRLSELKLPLSAVPVDERRASDAPAETDADRIPVPHPTEALQQ
                     LAATALGIARQDIARLQVFKRSFDARKAELLVVYIVDIALADPSGEAALLAKFAGKPH
                     ITATPDMGYHPVGHAPADLTERPVVVGFGPCGMFAALVLAQMGFKPIVLERGKTVRER
                     TKDTWGLWRKKTLNPESNVQFGEGG"
BASE COUNT          730 a         1245 c         1181 g          745 t
ORIGIN      
        1 ctgccggttc catgagctgg gcacctcaag ccctgcctga ccgctggcgc gatacccgcc
       61 ccaataccga tggttcggcc tggtaccgga tgcagtttga gcgcgatgct ctgggtcagg
      121 cattgcaggc cgtgttcatc ccccatgcca gcgtgaatgc ggaggtctac ctcaatggcg
      181 tgttgctggg cactggcggc agttttgagg agcccctctc ccgcacctgg aaccgccccc
      241 ggatgtacct cattgcgccg gaggtattgc agaccggccg caatacgctg gatgtccgtc
      301 tcaaggccca cctttacacc caggccagcc tgtacccacc cagaatcggt ccggaaaagg
      361 aaatccggga cctgtacgaa caggcctatt tcctgcgcat caccttgaac cagattgcca
      421 gcatcctgat tgccgtcgtg ggcctgctga tgctcaacat gtggtggcgc agacgccagg
      481 acacggccta tggctacttt gcggcgtccg ccctggtctg ggcggtgcag tccaccaacc
      541 tgtatgtgct tgacccgccg ttggacacgg cgcattggga aattctggtc aatgcgagct
      601 ttcagatctt ttctgcactg ctgctgatct cgatgctgcg ctacgtccgg gcagagtgga
      661 agcccctcaa cctgctgttg tggtcgtccc tgctcgcatc cccgctggcc atggtgctcg
      721 ctccgcccga gtccttcctg cgcgtcaccg cagcattgca catgtgcacc ctgatcggct
      781 cggtaggctc tctggtcctg ctgtttcgct ccgcctggag ggaccgcaac aaggacgcgc
      841 gccttctgtt ggccaccatg gggctgatcg tcctgtttgc agcacacgac tggctgctgc
      901 acagccagca cctctggttc tccggtgcat tgaagtggtt gccgggggag ttgtatttgc
      961 tgcagtacag cgcgccggtc gtttttctgg ccatcggctg gatcatgacg gtgcgcttca
     1021 tccaggtact caatgagttt gaggccttga gcgtggaact ggatcaacgc gtgcaggcca
     1081 aacatgcaca actggagcaa agcttcgaac gcttgcgtgc actggaacgt gagcgggccg
     1141 tgcaggatga gcgcgaacgc atccacagcg acctgcacga cgatgtgggc gccaaactgc
     1201 tgtcgctggt gtaccgtgcc ggctcgcccg aggcggccga cctcgcccgt tccgcactgc
     1261 aggacctgcg ggatgtggtg tcccgggtgg gctccggcag cttccagctg gaagatgtgc
     1321 tggccgacat ccgcgcggaa tgcgagcagc gtctgtccgg ggcatcaatc gcactggaat
     1381 ggcaccagac cgaagggatt gcgccgatcg agctgacaca accgcacgca ttgaatctgg
     1441 gccgcatcgt gcgggagacc atctccaacg ccattcgcca tgcccaggcc agcgccgtta
     1501 ccgtacacat cacgcccgat ggtgaaggac tgcacatcga gatctgcgac aacggtatcg
     1561 ggggcgcggt ggacggccat gtgccaggac gaggcttgcg caacatgcag gcgcgcgcgc
     1621 aactgatcgg cgcgcaactg gaacgctttt cggtgctgcc gcagggttgc ggcgtgcgcc
     1681 tgacgatccc gtccttcacg ggaacctaaa atcagggctc tgcttcacta ctatcagaca
     1741 catgccgcat attctgctgg ctggcattac gtgctgggtt tttatccaaa tgcacaaaaa
     1801 cgggatcttt ttcattcaac acaccatttt cgtgcgttac gcacctattt cgtgcatttt
     1861 ccaatgcgaa atgcatgttg cacataatgt catgcacttt tatggagcat gcagcaatga
     1921 aatacccgtc ggtccgcgcc tttttgcaac acgtcgagca acgcaacccc ggccagcctg
     1981 agttcctcca ggcggtcacg gaggtgatgg agagcctgtg gcccttcatc gaaaagcacc
     2041 cccgttatgc cgagcaaggc ctgctggacc gtctggtgga gcccgaacgc atcgtcatgt
     2101 tccgcgtatc gtgggtggat gaccacggca cggtgcaggt caaccgcggc taccgcatcc
     2161 agcacagcat ggccattggc ccctacaagg gcggcctgcg cttccacccc tcggtcaccc
     2221 tgtcggtact gaagttcctg gctttcgagc agactttcaa gaacgcgctg actaccctgc
     2281 ccatgggcgg tggcaagggc ggctcggact tcgaccccaa gggcaagagc cccgctgaag
     2341 tgatgcgctt ctgccaggct ttcgccagcg agcttttccg ccatattggc gccgacacgg
     2401 acgtccccgc tggcgacatc ggcgtgggtg gccgtgaagt gggctacatc gctggcatgt
     2461 acaaaaagct cagcaaccgc gccgactgcg tgttcacggg gaagggcctg tccttcggcg
     2521 gcagcctgat ccgccccgaa gccaccggct acggcacggt gtatttcgcc gatgaaatgc
     2581 tcaaaacccg gggcaagtcc ttcgaaggcc tgcgcgtgtc ggtgtccggc tctggcaacg
     2641 tggcgcaata cgccgtagag aaggccatgc agttgggcgc caaggtcatc accgtgtccg
     2701 attcgagcgg caccatcatc gacgaagacg gcttcacacc cgcgaagctg gccattctga
     2761 tggaggtgaa gaaccacctc tatggccgcg tgagcgacta cgctgcccgc acgggcgtca
     2821 agttcgaggc cggtgtgcgt ccctggcatg tcaaggtgga tgtggccctg ccctgcgcca
     2881 cgcagaacga gctggacgag aaggatgcaa aaaccctcat ccgaaatggc gtgctgtgcg
     2941 tggccgaagg cgccaacatg ccctcgacca tcgaagccgc caaggccttc gaggcagcgg
     3001 gcgtgctgta cgcaccgggc aaggccagca acgctggcgg cgtggccacc tcgggcctgg
     3061 agatgagcca gaacgccatg cgtctgtcct ggacccgtga cgaagtcgat gcgcgcctgc
     3121 tgcaaatcat gcagggcatt cacgcggctt gcctgcaata cggcaagcgt gccgatggca
     3181 gcgtgagcta catcgacgga gccaatgtgg ccggcttcgt gaaggtggcc gacgccatgc
     3241 tggcacaagg cgtgatctaa ccccagagct ttgctccccg gccccgcagc atggcagtgc
     3301 tgcggggccg tatcattggt gccctgcttg ataaccacga gcccgccatg atccgactct
     3361 ccgaacttaa actcccgctc tctgcagtcc ccgtcgacga acgccgcgca tccgatgcgc
     3421 ccgccgaaac cgatgccgac cgcattcccg ttccacatcc gacagaagca ctccaacagc
     3481 tggcagcaac tgctcttggc attgccaggc aggacatcgc caggctccag gtcttcaagc
     3541 gcagcttcga cgcgcgcaag gccgagctgc tggtggtcta catcgtggac atcgcgctgg
     3601 cagatccttc aggcgaagct gccctgctgg cgaaatttgc cggaaaaccc cacatcaccg
     3661 ccacgcccga tatgggctac cacccggtcg ggcatgcacc ggcggacctg accgagcgcc
     3721 ctgtcgtggt gggctttggc ccctgcggca tgtttgccgc actggtgctg gcgcagatgg
     3781 ggttcaagcc catcgtgctg gaacgtggca aaaccgtgcg cgagcgcacc aaggacacct
     3841 ggggcctgtg gcgcaagaag acgctcaacc ccgagagcaa cgtgcagttc ggcgagggcg
     3901 g
//
LOCUS       DAOSJM010000227         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_20532_length_3897_cov_3.682978, whole genome shotgun sequence.
ACCESSION   DAOSJM010000227 DAOSJM010000000
VERSION     DAOSJM010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20532_length_3897_cov_3.682978"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="PKX82_08650"
     CDS             complement(<1..592)
                     /locus_tag="PKX82_08650"
                     /inference="COORDINATES: protein motif:HMM:NF019077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HOF52362.1"
                     /translation="MDKRDFLWTSAALVAGACFSQTVLAQRSLPPETVNNFNGVTLAA
                     AWRQAQEGAEAKDFVGLLRADWDAAQVQVQAALAVPSRAHGLLGVADGGFVAVASRPG
                     SWMVRCDAQGSAVQWLRMDSEAEGRTLDGHVCASADGQWLYTAETHPHSGQGWVSARD
                     PVSLRKVAQWSTFGIGPHQLLLDASGALMVANGGLLR"
     gene            complement(612..1766)
                     /locus_tag="PKX82_08655"
     CDS             complement(612..1766)
                     /locus_tag="PKX82_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HOF52363.1"
                     /translation="MAPLRSGSRRRRLWELEHVCHCPLIGVSLPMDALRRLVNKALGG
                     GVVANDYEVHAGAVSECARRCKLSELMQAELEARYAREVQVFRSAKSALAVAELWIKY
                     VKAGDVAGAFWAALSHPRCDGPLQEAMCQDLHMFQHQAGAGVRLEIARFNALAEENAV
                     LGRELGKVQERITRVMAQKTTDIDRLNGELVLVRAEAIAKDTRIAYLAQDLEALQASI
                     PQLEDRQRLQKRVDQLTQRQQELEVHNADLRRKLATAERAAVERSGLEWPVAEAAALQ
                     DVHPVVMHLHEKVVLCVGGRSGNISNYRDVVERVGGRFSHHDGGIEDSASVLDANLAA
                     ADLVICQTGCISHNAYWRVKDFCKRTGKRCVFVENPSASSLERGLAQVSE"
     gene            complement(2108..2398)
                     /locus_tag="PKX82_08660"
     CDS             complement(2108..2398)
                     /locus_tag="PKX82_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52364.1"
                     /translation="MHSTPALHHNEEYTMPSAEALLAGTLALMTGHVQASCCAHRELM
                     AAKIVSNLSALADVPVLSPAFKAMLQNLRLRWQNQGPATERSLWHRSPDAVQ"
     gene            2504..2689
                     /locus_tag="PKX82_08665"
     CDS             2504..2689
                     /locus_tag="PKX82_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52365.1"
                     /translation="MRAASTQALPGVGALACGGNRVVAGADRAAHLYAAPDASPAIHA
                     LSTPWDNHAVLLKDATT"
     gene            2810..3025
                     /locus_tag="PKX82_08670"
     CDS             2810..3025
                     /locus_tag="PKX82_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOF52366.1"
                     /translation="MIVCVCHRVSDREITRHAQEGKNFDDIQFELGVASQCGRCEECA
                     RNVIDQCHATQCATRPVWSPISMGMSM"
     gene            3237..>3897
                     /locus_tag="PKX82_08675"
     CDS             3237..>3897
                     /locus_tag="PKX82_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOF52367.1"
                     /translation="MLLMGSFGVHAQTTNTTDVKSLNPVTIVEKAEESQGKDSLRATT
                     TTIGKGKQELRDIPQSITVVTEKLVDDRNLDTLKDVLHNTAGVTFQAAEGGEEDIRLR
                     GFSLATSGDIFIDGMRDAAFYERDTFNNDRIELLRGSASMLFGRGSTGGAVNQVSKQP
                     RAIDESELTTTLGSYNYRRVTGDFNIRTGEDAGLRITAMATKADSNGAGSSIDKRGMA
                     AA"
BASE COUNT          798 a         1254 c         1069 g          776 t
ORIGIN      
        1 cgcgcagcag cccgccattg gccaccatca aagcacccga agcatccagc agcagctggt
       61 gcggcccaat accaaaagtg gaccattggg ccaccttgcg caggctcacg ggatctcgcg
      121 cggacaccca accctggcct gaatgggggt gggtctcggc ggtgtacagc cattgcccgt
      181 ccgcactggc gcacacatgg ccatccagcg tgcgaccttc ggcttcactg tccatgcgca
      241 gccactggac ggcgctgcct tgcgcatcgc agcgcaccat ccagctgcct ggccggcttg
      301 ccaccgcgac aaaaccacca tccgcaactc ccagcagtcc gtgcgcacgg ctcggcactg
      361 ccagtgccgc ttggacttga acctgcgcgg catcccaatc agcgcgcagc aggccgacaa
      421 agtctttcgc ttctgcgcct tcctgtgctt ggcgccatgc agcggcgagg gttactccgt
      481 tgaagttgtt aacggtctcg ggtggcaagc tgcgctgcgc aagaaccgtt tgcgaaaaac
      541 aggcaccagc caccagcgcg gcactggtcc acaaaaaatc acgtttatcc atgttgatcc
      601 tgaaaacacc gttactctga aacctgcgca agcccacgct ccagcgaaga tgcgctgggg
      661 ttctcgacaa acacgcaacg tttgcccgta cgcttgcaaa agtccttgac gcgccaatag
      721 gcgttgtggc tgatacagcc ggtctggcaa atcaccaaat cggcggcggc caggttggcg
      781 tccagcacgg aggcgctgtc ttcgattccc ccatcgtggt gggaaaaccg accaccgaca
      841 cgctccacca catcacggta gttggagata ttgccactgc gcccccccac gcacagcacc
      901 actttctcgt gcagatgcat caccacaggg tgtacgtcct gaagtgccgc tgcttctgcc
      961 acaggccact ccagaccaga gcgctcgacc gccgcccgct ctgcagtggc cagcttgcga
     1021 cgcaggtcgg cattgtgtac ctccagttcc tgctgacgct gggtcagctg atccacccgc
     1081 ttctgcaggc gctgccggtc ttccagttgg ggaatggagg cctgcagtgc ttccaggtcc
     1141 tgggccaggt aggcgatgcg cgtgtccttg gcaatggcct ccgcacgcac cagcaccaac
     1201 tcgccattca gacggtcgat gtcggtcgtc ttctgggcca tgacgcgggt gatgcgctcc
     1261 tgcaccttgc ccagttcgcg tcccagtacc gcgttctctt cggccagcgc gttgaagcgg
     1321 gcaatttcca ggcgcacacc cgcccccgcc tggtgctgga acatgtgcag gtcctggcac
     1381 atggcttcct gcagcggtcc atcacaccgc ggatgcgaca gtgccgccca gaaggcgccc
     1441 gctacatcgc ccgccttcac atacttgatc cacaactccg ccacagccaa tgcacttttg
     1501 gccgatctga atacctgcac ttcacgcgca taccgggcct ccagttccgc ctgcatcagc
     1561 tcggagagct tgcagcggcg tgcacattcc gaaacagcac ccgcgtgcac ctcgtagtca
     1621 ttggccacta cgcccccgcc cagcgccttg ttgaccaggc gtcgcaatgc atccatcggc
     1681 aggctgacgc caatcagggg acagtggcac acatgttcga gttcccacaa ccgccggcgc
     1741 cgtgagcccg aacggagcgg tgccacatgg tcgtggtggc agtggatctc cggggtctgc
     1801 cattttctgc gcgtggcatg ctctccgggc gctatattat tcatagctgc ttgcgcagta
     1861 tttgtaaggg ctagaggcat atttgactcc taatatgttg attccacagc attcccggca
     1921 agcgttggct gggcggccag acgcgtgcgc catgccagca ccgggcgctt gccgcttcga
     1981 cgctgtgatt tgccttcgtg accctcgggc tgcggggcgc aacaggcgca ccatgtgcgg
     2041 tagcggccgt gtttgccagc tggaagggcg gacggttggg cgtcgagcag agcttgcacg
     2101 gccgtattca ttgcacggca tccgggctgc gatgccaaag gctgcgctct gtggctggcc
     2161 cctggttttg ccagcgcagg cgcaggttct gcagcatggc tttaaaagca ggcgacaaca
     2221 cagggacatc ggccagcgca ctcaaattgg aaacaatctt ggccgccatc agttcgcggt
     2281 gcgcacagca actggcctgc acatggcccg tcatcaacgc cagtgtgccg gccagcaggg
     2341 cctcggcact gggcatggtg tactcctcgt tgtggtgtag cgccggggtg ctatgcatgt
     2401 tctctccttg gttctttctg gtactgaact tgaatgcgta cgcaggtcag tatatgcgaa
     2461 tcattctcat ttgcaaaaac attctattgg cagggatttt tatttgagag cggcatccac
     2521 ccaggcactt cctggcgtgg gcgccctggc ctgcggtggc aatcgcgtgg tggcgggcgc
     2581 agaccgtgct gcccatctgt acgccgcacc agatgcatca ccggccatcc acgcactgtc
     2641 cacaccgtgg gacaaccacg cggtgttgct taaggacgca acgacctgaa ttgaccgggc
     2701 gtacgtgtcc gaaagcactc atttattgca ggattttttt gaatcacaat gcaaatgcga
     2761 ctaactctca tttagaatac aaacatcgat gaattgggag aacgttctta tgatcgtttg
     2821 cgtttgccac cgtgtttctg accgtgaaat cacccgccac gcgcaagaag gcaagaactt
     2881 tgatgacatc cagtttgaac tcggcgtagc ctcccaatgc gggcgttgcg aagagtgcgc
     2941 cagaaacgtc atagaccagt gccacgccac ccaatgcgca accagacccg tctggagccc
     3001 catcagcatg ggaatgtcca tgtaattcat cgcctttttt ttgtcccttg ttgggagtga
     3061 ttcccgtttc cattttttta accgttagca agccaaagtc aggaatccgc agccgcggac
     3121 cactgccata gccagccacg ccaacccgca caccctgcgt tactgctatg acacaccaca
     3181 cttcacaaac tcaaaagaca aacccagcca gtcctgtgct gttgcccctt ggcgccatgc
     3241 tgctcatggg ctcattcggt gtgcacgcgc aaaccaccaa cacaaccgac gtcaagtcac
     3301 tcaatcccgt caccattgtt gaaaaagccg aagagtccca aggcaaggac agtctgcgcg
     3361 ccaccaccac caccatcggc aaaggcaaac aggaactgcg cgacattcct cagtccatca
     3421 ccgtggtcac cgagaagctg gtagacgacc gtaatctgga taccctgaaa gatgtgctgc
     3481 acaacactgc gggtgtcacc tttcaggcag ccgagggtgg cgaagaagat attcgattgc
     3541 gcggtttctc tctggcaacc tcgggcgata tttttatcga cggcatgcgt gatgcggctt
     3601 tctacgagcg tgacaccttc aacaacgacc gtatagaact gctgcgcggc tcagcttcca
     3661 tgctgtttgg ccgcgggtcc actggcgggg ccgtgaacca ggtcagcaaa cagccacgcg
     3721 ccattgatga aagcgaactc acgaccacgc tgggcagcta caactaccgc cgcgtaactg
     3781 gtgacttcaa catccgcacc ggcgaagacg cgggcttgcg catcactgcc atggccacca
     3841 aagccgacag caatggtgct ggcagcagca tcgacaaaag aggtatggcc gccgcct
//
LOCUS       DAOSJM010000228         3856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_20793_length_3856_cov_3.643515, whole genome shotgun sequence.
ACCESSION   DAOSJM010000228 DAOSJM010000000
VERSION     DAOSJM010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20793_length_3856_cov_3.643515"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PKX82_08680"
     CDS             <1..288
                     /locus_tag="PKX82_08680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF52368.1"
                     /translation="ENDLFGRWGGEEFIALMPDTGMAAAVDAAERVRKAMHTLQFAGT
                     RGPFGVTVSIGVAQLETNETADSLTHRVDKALYAAKHAGRDLVLSYAPQQE"
     gene            522..>3856
                     /locus_tag="PKX82_08685"
     CDS             522..>3856
                     /locus_tag="PKX82_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF52369.1"
                     /translation="MHWNFLQSRSLKVKITLFALVIFVASFWSLAYYASRMLRSDMEQ
                     LLGAQQYEAASFLATSVQQQLTERLTALETVAGEVRGSLLAGTTGFTGALESRPVLQR
                     LFNGGVVVVLPDGTLAAPLVPAYRPLQTAADFDRSFFQRALALAGGAGTSYAGVNLQG
                     IPFVATVFPVKDNSGGVMGALVGLTDLGTANFLDAIAQSDRAGHGAYLLVVDPQRRTI
                     LASSDKTRVMQVVQPGASTLADRFIDGYEGSGIAVNPSGSEVLSSAKGVPGVGWVVTV
                     SLPADRAFAPIGAMQQRMFFGAALLTLLAGVLTWWMLRRQLAPMLSTVRTLTTLSNTG
                     QSPKQLPVASQDEIGSLVASFNHLLDTMAQRDALHRQILDTSSVAIFLVDMHGRITQA
                     NQPMARMFGRALESLEGAEYVSLVHPREREEGREKMLALLGSKIAVVDVDRLYWREDG
                     TEFWGRLTGRRFVDASGQDRGLVGVIADITERKKMQHYELFRSRILEMLTVSQPLDSV
                     LVAMVQGVEQILTSSLCSILVLDEQGKHIHQGYAPSLPDAYNAALEGLAIGIGAGSCG
                     AAAFTGERVVVVDIATHPYWAPYRDLAASAGLAACWSQPFRDSSGRVIGTFAIYHRTP
                     HAPTATDITIIEQSAHLASIAIERSQDARKLQESEAHFRLLTEDVSDVVWKTDKDFVF
                     TYISPADERLRGYRADEVVGRKVSDLFNQDGIATVRRAIQQREADAGSPSPLAASFEA
                     QQRCKDGRWVWTEILAIAERDAGGIVTGYHGITRDITARRQSEESLQLAASVFSHAQE
                     GILITNLDGIIIDVNAAFTRITGYSREEVLGSNPRMLSSGRHGDDFYAALYRELQDKG
                     QWQGEIWNRRKNGEMYAQTQTISTVNDAQGRALHYVALFSDITDQKMHQSQLEHIAHF
                     DALTNLPNRVLLGDRLHQGMAQSARRGEILAVAFLDLDGFKAINDQFGHDAGDHLLVA
                     LAARMKSALRDGDTLARIGGDEFVAVIADIADTAASVPLLHRLLAAAAQPVQVGAHTL
                     QVSASVGVTFFPQSHEVDPDQLLRQADQAMYQAKLAGKNRYHVFDAAHDSSIRGHHES
                     IEHIRRALAQKEFVLHYQPKVNMR"
BASE COUNT          736 a         1090 c         1251 g          779 t
ORIGIN      
        1 gagaatgacc tgtttgggcg ttggggtggc gaggagttca ttgcactgat gccagatacg
       61 ggcatggcag cggcggtaga cgcggcagag cgggttcgca aggcgatgca taccttgcag
      121 tttgcgggta cccgagggcc tttcggtgtc acagtctcca tcggtgtggc acaactggaa
      181 acgaatgaaa cagccgacag tctgacccat cgggttgaca aggccctcta tgccgccaag
      241 catgctggac gggaccttgt gctgtcctac gcgccgcagc aggagtaggg ttatgccaca
      301 atgcattgat gcagcttcag cacacacttc ggaaaccgca tcgtttgcaa tacttggtga
      361 aaagcaggct tgttttgcca cggggacagg cctatgcttt cgctgtcgag cgcatggcat
      421 cggcttcact gttgcccgac gcccaagagg gaatttctgc atttcttgag gaatttcctc
      481 cgaatcaccg gtaggacttc ctgaaactga ctggcaaccg catgcactgg aattttcttc
      541 aatcgcgctc gctcaaggtc aagataacgc tgttcgcgct ggtgatcttt gtggccagtt
      601 tctggtcgct cgcctattac gcgagccgca tgctgcgctc ggacatggaa cagctactgg
      661 gagcgcagca gtatgaagcc gcgtccttcc tggctaccag tgtgcaacag caactcactg
      721 agcggttgac ggctctggaa accgtggcgg gagaggttcg gggcagtctg ctggccggga
      781 cgaccggatt cacgggtgcc ctggagagtc gcccggtact tcagcggctc ttcaatggcg
      841 gggtagttgt tgtattgccc gacggcacgt tggctgcacc gttggtccct gcctaccgtc
      901 cactgcagac ggctgcggac tttgaccggt ctttttttca gcgtgcactg gcgctggcag
      961 ggggggctgg cacttcctat gcgggggtga acctccaggg catccccttc gtcgccacgg
     1021 tgtttcccgt gaaggacaac agtggcgggg tcatgggagc gctggtgggt ttgacggatc
     1081 tgggcacggc caacttcctg gatgcgattg cccaaagtga ccgcgccggt cacggcgcct
     1141 acctgctggt ggtggacccg caacgccgga cgattctggc ttcttcggac aagacgcggg
     1201 tcatgcaggt ggtgcagccg ggtgccagta cattggcaga ccggttcatc gatggttacg
     1261 aggggtcggg tattgcagtc aaccccagtg ggagtgaagt gctttcctct gccaagggcg
     1321 ttcccggtgt gggttgggtg gtgacggttt ccctgcctgc tgatcgggcc tttgcgccga
     1381 taggtgccat gcagcagcgc atgttcttcg gagcggcact cctgacgctg ctggctggtg
     1441 tgctgacgtg gtggatgttg cgacggcaac tcgcgcccat gctgtcgaca gtccgtacgc
     1501 tgacgaccct gtccaatact gggcaatcgc ctaaacaact gccagtggcc agccaggatg
     1561 aaatcggaag tctggttgcc agcttcaacc acctgctcga caccatggcg cagcgcgatg
     1621 ccctgcaccg gcagattctg gatacctcca gtgtggccat tttcctggtg gacatgcatg
     1681 gccgtattac ccaggccaac cagcccatgg cccggatgtt tggccgggct ctggagtcgc
     1741 tggagggcgc cgagtatgtg tcgctggtac accccagaga gcgcgaagag ggccgggaaa
     1801 aaatgcttgc gctgctgggc agcaaaatcg cagtggtgga tgtggaccgg ttgtactggc
     1861 gggaagacgg gacggaattc tggggacgtc tgaccggacg gcgctttgtc gatgccagcg
     1921 gacaagatcg tggtttggtg ggcgtgattg cagacatcac cgagcgcaaa aaaatgcagc
     1981 actacgagct gtttcgtagc cgcattctgg aaatgctcac ggtcagtcag ccgctggaca
     2041 gcgttctggt ggccatggtg cagggcgtgg aacagattct cacctcgtcc ctgtgcagca
     2101 tcctggtgct ggatgaacag ggaaaacaca tccaccaggg atatgcacca agccttcccg
     2161 atgcgtacaa cgcagcgctg gaggggctgg ccattggaat cggcgcggga tcgtgtggcg
     2221 ctgcggcctt taccggtgag cgcgtggtgg tggtggatat cgccacccat ccgtactggg
     2281 caccctatcg ggatctggcg gccagtgcgg ggctggccgc gtgctggtcg caaccatttc
     2341 gtgactcgtc tggccgggtg attggtactt ttgccatcta ccaccgaacc ccccatgcac
     2401 cgacggcgac ggacatcacc atcatcgaac agtcggccca tctggccagc atcgccatcg
     2461 agcgcagcca ggatgccagg aaactgcagg agagcgaagc ccatttccgt ctgttgacgg
     2521 aggatgtttc ggacgtggtg tggaagacgg acaaagactt cgtgttcacc tacatcagtc
     2581 ctgcagacga acgcttgcgt gggtaccggg ccgatgaggt ggtagggcgc aaagtgtctg
     2641 atttgttcaa tcaggacggt atcgccactg tcaggcgggc gattcagcag cgcgaagccg
     2701 atgcaggtag tcccagtcca ttggcggcat cgttcgaagc gcagcagcgg tgcaaggatg
     2761 gccgctgggt ctggaccgaa atactggcca ttgccgagcg ggatgccggt ggcatcgtca
     2821 ccgggtacca cggcatcaca cgcgatatca cagcgcgcag gcaatctgag gaatcgctgc
     2881 aattggcggc cagtgttttc agtcatgcgc aggaaggcat tctgatcacc aatctggacg
     2941 gcatcatcat tgacgtgaat gcggcgttca cccgaattac cggctacagc cgggaagaag
     3001 tgctggggag caacccgcgc atgctgagtt cgggacggca tggggatgat ttttatgccg
     3061 ctctctaccg ggagttgcag gacaagggcc agtggcaggg tgagatatgg aaccgccgca
     3121 agaacggcga gatgtatgcc cagacccaaa ccatcagcac ggtgaacgat gcgcagggca
     3181 gggcgctgca ctatgtggcc ctgttctcgg acatcaccga ccagaaaatg caccagagcc
     3241 agctggagca catcgcgcac tttgatgcgc tgaccaacct gcccaaccgg gttttgctgg
     3301 gagaccgctt gcaccagggc atggcacagt ccgcgcgccg tggcgagatc ctggcggtgg
     3361 cttttctgga cctcgacggt ttcaaggcca tcaacgacca gtttggccac gatgccggtg
     3421 accacctgct cgtggccctg gctgcccgta tgaagtctgc cctgcgtgac ggcgatacgc
     3481 tggcgcgcat cggtggggac gagttcgtcg cggtcattgc cgatattgcg gacacggcgg
     3541 ccagtgtgcc gctgctgcac cggttgctgg ctgctgccgc gcagcctgtg caggtgggcg
     3601 cacatacctt gcaggtgtcc gccagcgtgg gcgtgacgtt tttcccgcag tcccacgagg
     3661 tcgaccccga ccagctgctg cgccaggccg accaggccat gtaccaggcc aagctggcgg
     3721 gcaaaaaccg ctaccacgtg ttcgatgctg cgcacgacag cagcatccgt ggccaccacg
     3781 agagcattga gcacattcgc cgtgcgctgg cgcagaagga atttgtgctg cactaccagc
     3841 ccaaggtcaa catgcg
//
LOCUS       DAOSJM010000229         3847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_20859_length_3847_cov_3.934335, whole genome shotgun sequence.
ACCESSION   DAOSJM010000229 DAOSJM010000000
VERSION     DAOSJM010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20859_length_3847_cov_3.934335"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PKX82_08690"
     CDS             complement(<1..973)
                     /locus_tag="PKX82_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOF52370.1"
                     /translation="MTQPRAWWLLWAGVLGALALTVVGICVGSAGFENLLQPLLHPEL
                     DPGRSAMAQQIVWEIRLPRTLGAWAAGALLGLAGAVAQGLFRNPLADPYLLGSASGAS
                     LGVALALAALGGGAGMLGGNMMNGSVPVSVFSSSVWVRLGLTGAAFCGAVLAVLLTLV
                     LSRGVGHTLRLLLAGVVVGVVLGAMTHLVLLFSPDSLQAMQAFMLGSTAFVGWTSCVL
                     MAGVWCVCVLVAWLLARVLDGLSLGDATAVSLGLPIVPMRAALVAVLALATGTAVAQT
                     GLIAFVGLAAPHLVRSVVKTTHGHLMLLSSLVGGVLLMAADTLARGLI"
     gene            complement(970..1827)
                     /locus_tag="PKX82_08695"
     CDS             complement(970..1827)
                     /locus_tag="PKX82_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOF52371.1"
                     /translation="MAALALVFGVVGGSHALQIVDDRGVTVTLPASPQRIVSLLPSLA
                     ETVCALDRCQRLVGVDRYSVYPESLRKLPQVGGGLDPNIEAIVALRPDVVLMSRSSRA
                     GARLEALGLKVVALEPKTHADVQRVILKVGQLLEVPDAQRIWRAIDAGVSAAAQSLPA
                     HVRGVRVYFEVNQGPYAASEASFIGETLTRLGARNVVPGALGPFPKLNPEFVVRANPD
                     LIMIGQRSVDALQTRPGWQSIRALREKRVCDFPADQADVLVRPGPRMAEAARLMARCL
                     ESKAPGAKP"
     gene            complement(1965..2558)
                     /gene="cobU"
                     /locus_tag="PKX82_08700"
     CDS             complement(1965..2558)
                     /gene="cobU"
                     /locus_tag="PKX82_08700"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOF52372.1"
                     /translation="MSTLSIAKSELILGGQKSGKSRRAELLARQWLAQSADHRAVLIA
                     TAQPWDEEMRQRIARHQADRAVRVPGMRTVEEPLALAEAIQAHSTAQTLVVVDCLTLW
                     LTNLLMPADAAYCEENRPPAPVEPAQKAPLLIAIAQAAGPVVLVGNEIGLGVIPMGRE
                     VRAFVDALGRLNQDVAAACQRVTLMAAGLPLTLKETT"
     gene            complement(2555..3406)
                     /locus_tag="PKX82_08705"
     CDS             complement(2555..3406)
                     /locus_tag="PKX82_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF52373.1"
                     /translation="MTQDLLDFPLGPQRIVCMTEETTEWLYLLGQEQRIVGISGYTVR
                     PRRARDEKPRVSAFTSAKIDKILALQPDCVLGFSDMQADIAAELVRRGVQVTIFNQRS
                     VAEIFSMLFQLAAMVGEAEHGAQRIAQMQADLRAMQADVAAKVAAGARRPKVFFEEWD
                     MPHISAIRWVSELLGIAGGDDCFPELARESLGKNRIIADGAEIVRRNPDIILGSWCGK
                     KFRPENVAARTGWSAVNAVRHQQLFEIKSPDILQPGPAALTDGVQKMHQIILRWMDAD
                     LQGAFHA"
     gene            complement(3403..>3847)
                     /locus_tag="PKX82_08710"
     CDS             complement(3403..>3847)
                     /locus_tag="PKX82_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HOF52374.1"
                     /translation="HAQTIAANTALRDSLAAHIASGKPVWAECGGMMALFDTLVTVDG
                     ERHAQWGLLPGEVTMHKRLAALGPQQLRLSSGTLRGHTFHYSTTATPLQARLRTARPD
                     SDPMPDAGEAVWQQGAVRASYFHAWFPSCPEAVVELFTAPQESVA"
BASE COUNT          706 a         1384 c         1186 g          571 t
ORIGIN      
        1 caatcaggcc acgcgccagg gtgtcggccg ccatcagcag cacgccgccc accaggctgg
       61 acagcagcat caggtggcca tgcgtggtct tgaccaccga gcgcaccagg tgcggtgcag
      121 ccagtcccac aaacgcaatc agtccggtct gggccacggc ggtaccggtg gccaacgcca
      181 gcactgccac cagggcggca cgcatgggga cgatgggcag ccccaggctg acagcggtgg
      241 catcgcccag gctcagacca tcgagcacgc gcgccagcag ccaggccacc agcacgcaca
      301 cacaccacac accggccatc agcacacacg aggtccagcc gacgaaagcc gtggagccca
      361 gcatgaaggc ctgcatggcc tgcagcgagt cgggcgaaaa cagcaacacc agatgcgtca
      421 tggcgcccag caccacgccc accaccacac cagccagcag caggcgcagt gtgtggccca
      481 caccgcgcga cagcaccagc gtcagcagca ccgccagcac cgcaccgcaa aaagctgcac
      541 cggtcaggcc caggcgcacc cacacgctgc tggaaaacac gctcaccggc acgctgccat
      601 tcatcatgtt gccacccagc atgccggcac cgccgcccag cgcagccaat gccagcgcca
      661 cacccagcga cgcacccgac gcactgccca gcaggtacgg gtcagccagc ggattgcgaa
      721 acagaccttg cgcgaccgca ccggccagcc ccagcaaagc cccggcggcc caggccccca
      781 gcgtgcgcgg caggcggatt tcccagacga tctgctgcgc catggccgag cggcccgggt
      841 ccagctccgg atggagcaag ggctgcagca ggttctcgaa cccggcacta cccacgcaga
      901 tgcccacgac ggtgagcgcc agcgcgccca gcacgccggc ccacagtagc caccatgcgc
      961 gcggctgcgt catggtttgg cccccggtgc cttgctttcc aggcagcggg ccatcaagcg
     1021 tgcggcctcg gccatgcgtg gaccggggcg caccagcaca tcggcctggt cggccgggaa
     1081 gtcacacacc cgcttttccc gcagtgcacg gatgctctgc cagcccggac gcgtctgcag
     1141 ggcatcgacg ctgcgctggc caatcatgat caggtccggg ttggcccgca ccacgaattc
     1201 cgggttgagt ttggggaaag ggccgagcgc acccggcacg acattcctcg cccccaggcg
     1261 ggtcagtgtt tcgccaatga acgacgcctc gctggccgca tacggcccct ggttcacctc
     1321 gaaatacacc cgcacgccac gcacatgcgc cggcagcgac tgggccgcgg cagacacccc
     1381 ggcgtcaatg gcgcgccaga tgcgctgggc atcgggcact tcgagcaact ggccgacctt
     1441 gaggatcacg cgctgtacat cagcatgggt cttgggctcc agcgcgacca ctttgaggcc
     1501 cagcgcctcc agccgggcgc cagcacgcga cgacctggac atcagcacca catcgggccg
     1561 caacgccacg atggcttcga tgttcgggtc cagcccacca cccacctggg gcagcttgcg
     1621 cagactttcc ggatacaccg agtagcgatc cacccccacc agccgctggc atcggtccag
     1681 cgcgcatacg gtttctgcca acgagggcag caggctgacg atgcgctgcg gcgaggctgg
     1741 cagcgtgacg gtgacgccgc ggtcatcgac gatctgcaag gcgtgcgaac cgcccacaac
     1801 gccgaacacc aatgccagcg ccgccagaag gcgggacgcc caggcgcttt gctccgtgtc
     1861 ccccgcccgc tgcacgggct cctcctttac ctgcgcaaaa cgcctgggcg ccccgcctcg
     1921 cggcaggtgc cataaccggc aaaagaatcc accgtaggtc ttgttcacgt tgtttctttc
     1981 aaggtcaagg gcagtccggc agccatcagc gtcactcgct ggcaggctgc ggccacatcc
     2041 tggttcagtc gccccagcgc atccacaaag gcccgcacct cgcgccccat ggggatgacg
     2101 cccaagccaa tctcgttgcc caccagaacc accgggcctg cggcctgcgc gattgctatc
     2161 aaaagaggag ctttctgcgc aggttccacg ggggctggag gcctattttc ttcacaatac
     2221 gccgcgtcag caggcatcag caggttggtc agccacagcg tcaggcaatc caccaccacc
     2281 agcgtctggg cggtgctgtg cgcctggatg gcctcggcca gcgccagcgg ctcttccacc
     2341 gtccgcatgc cgggtacgcg cacagcgcgg tcggcctggt ggcgggcaat gcgctggcgc
     2401 atttcctcat cccagggctg ggccgtggcg atcaacacag cgcggtggtc cgccgactgc
     2461 gccagccact ggcgtgccag cagctcggct cggcgcgact tgccgctctt ctgtccgccc
     2521 agaatcagct cgcttttggc aatgctcagt gtgctcatgc gtggaaggct ccctgcaaat
     2581 cagcgtccat ccaacgcagg atgatctggt gcatcttctg gacaccgtcg gtcagcgcgg
     2641 ccggaccggg ctgcaggatg tcgggcgact tgatctcgaa taactgttgg tgacgcacgg
     2701 cgttgacagc actccagcct gtacgcgcgg ccacattctc gggccggaac ttcttgccgc
     2761 accaggaacc cagaatgatg tccgggttgc gccgcacgat ctcggcacca tcggcaatga
     2821 tgcggttctt tcccagcgac tcccgggcca gctcagggaa gcagtcatcg cctccggcga
     2881 tgcccagcag ctcggacacc cagcggatgg cgctgatgtg gggcatatcc cattcttcaa
     2941 aaaacacttt gggccggcgc gcgccagccg ccactttggc ggccacgtca gcctgcatgg
     3001 cgcggaggtc agcctgcatc tgcgcaatgc gctgtgcgcc atgctctgcc tcgcccacca
     3061 tggcggcgag ctggaacagc atggaaaaga tctcggccac gctgcgctgg ttgaagatag
     3121 tgacctgcac accgcggcgc accagttccg ctgcaatgtc ggcctgcatg tcggagaaac
     3181 ccagcacaca gtcgggctgc aatgccagaa ttttgtcgat cttcgcgctg gtaaacgcgc
     3241 tgacccgcgg cttctcgtcc cgcgcccggc gcggccgcac ggtgtagccc gagataccta
     3301 cgatgcgctg ctcctgcccc agcaggtaga gccattcagt ggtctcctcg gtcatgcaga
     3361 cgatgcgctg cggaccgagc gggaagtcca gcaggtcctg cgtcatgcca cactttcttg
     3421 tggcgcggtg aacagttcca ccaccgcctc ggggcacgac ggaaaccacg catggaaata
     3481 actcgcgcgc acggcaccct gttgccatac cgcctcgcca gcgtcgggca tggggtcgct
     3541 gtccgggcgc gcggtgcgca ggcgggcctg caacggcgtg gccgtcgtgg aatagtggaa
     3601 ggtgtggcca cgcagcgtgc cgctggacag gcgcaactgt tgtggcccca gcgcggccag
     3661 gcgcttgtgc atggtgacct cgccgggcaa cagaccccac tgcgcatggc gctcgccatc
     3721 cacggtgacc agggtgtcaa acagcgccat catgccgccg cactcggccc acaccggttt
     3781 gcctgaggca atgtgcgcgg ccaggctgtc gcgcagcgcg gtgttggctg caatggtttg
     3841 cgcatgc
//
LOCUS       DAOSJM010000230         3839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_20904_length_3839_cov_5.086152, whole genome shotgun sequence.
ACCESSION   DAOSJM010000230 DAOSJM010000000
VERSION     DAOSJM010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20904_length_3839_cov_5.086152"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PKX82_08715"
     CDS             <1..208
                     /locus_tag="PKX82_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOF52375.1"
                     /translation="LGMGLLQWRQRLRVVKAMALLERGQTVETIALDLGYGSASAFIA
                     MFKRLAGTTPDEYRKTAAGGIKGN"
     gene            291..1958
                     /locus_tag="PKX82_08720"
     CDS             291..1958
                     /locus_tag="PKX82_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOF52376.1"
                     /translation="MSQTHTSSGASARALLGDRVLMVAVGVSALASIGLGLQFIESGL
                     ALGFTAVLALLAVGIYVTAKGSVASRYVLTFVLVGFVALHIQLARGMIEFHFGVFVVL
                     AFLLVYLDWKLIIFGAAAFAVHHIAFDRLQAAGLGFFCLTEANFGLIVVHAVYVVVQS
                     AVEVVLAINMNRAARQGDELGVLVSSVNRADGISLDVRGVATETEGANALKATLSRMD
                     SAVSTVRSGAQSIEVACSEIASGNQDLSNRTEQTAANLQRTSSSMSALTGTVRQSADS
                     ARQANQLAMNASTVAAKGGDVVAQVVETMQGINESSRKISDIISVIDGIAFQTNILAL
                     NAAVEAARAGEQGRGFAVVASEVRSLAGRSAEAAKEIKALIGASVERVEQGTALVGQA
                     GATMTEVVTSIRQVTDIMGEISAATGEQAAGVAQVSQAVTEMDQATQQNAALVEQMAA
                     AASSLKSQAEELVQTVAVFGAAEGAHQTARQFRSAPVQTATPRVAPAAKPLNLSGSRP
                     APKAAPKAGVPKVGMSTKPVAPPQPPAVPKIQPKPAPAGGDEEWETF"
     gene            1955..2980
                     /locus_tag="PKX82_08725"
     CDS             1955..2980
                     /locus_tag="PKX82_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52377.1"
                     /translation="MTARPEHHRPRGFANTDPEVVIGNFPWYEMVWRSLRGDFKPLSA
                     PAEGYAAFAKAWSTPVNHARLAVRQTAPVVTWLGHVSMLLQVAGLNILIDPTLADCAG
                     PFGRVGAPRRVPAPLTPEQLPLIDVVLISHNHYDHLCDATLSRLVAAGQSPHFYVPLG
                     LGPWFMSRGMDRVTELDWWHQAPLKEGVTLHFTPSQHWSRRTPWDTNASLWGGFMVEW
                     ARQTEGEGGEPWRFLFPGDTGYSNDFKTIRERLGAVDFLALPIGAYLPRDFMKPMHVN
                     PADAVQLMLDLDAKQAMGVHWGTFLLTQEAFDHPPRDLAAALHDRGLPQERVWLMKHG
                     ETRAIPL"
     gene            complement(2999..3403)
                     /locus_tag="PKX82_08730"
     CDS             complement(2999..3403)
                     /locus_tag="PKX82_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HOF52378.1"
                     /translation="MTAVQALLGFAAWTLLLVTGVFLYRGLRFVTGTPINHWPRGAKP
                     ADDAPLVKRLEDAHANSLENLPIFAVIVLAAAALGKLDAIAALAPWVFYARVGQSLAH
                     LSGVGVLQVLVRATFWSVQLALFFWMLAVLLA"
     gene            complement(3445..>3839)
                     /locus_tag="PKX82_08735"
     CDS             complement(3445..>3839)
                     /locus_tag="PKX82_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOF52379.1"
                     /translation="FGVELFVKGDNVWFSEVSPRPHDTGMVTMITQWQNEFELHARAI
                     LGLPVSTALKSPGASAVIYGGVEAQGIVFDGVDDAMRVPNSEIRLFGKPESFVKRRMG
                     VALVHDADVEVARTQAKLAANKVKPRTA"
BASE COUNT          679 a         1186 c         1271 g          703 t
ORIGIN      
        1 cctgggcatg gggctgttgc agtggcgcca gcggctgcgc gtggtcaagg ccatggcgct
       61 gctcgagcgc ggtcagacgg tagagacgat tgcactggat ctgggctacg gcagtgcatc
      121 ggccttcatc gccatgttca agcggctggc agggaccacc cctgacgagt accgaaagac
      181 ggctgcaggc ggcataaagg ggaactgagg agtcgcattc ggtgctatcc ttttgtatac
      241 attttaggtt gcacgccttg gtgtgtggct attaacagga gacctgagcc atgagtcaaa
      301 cacatacatc ttccggcgcc tcggcgcgcg cgctgctcgg cgatcgggtc ctgatggtgg
      361 cggtgggcgt cagtgccctg gccagcattg gcctggggct gcagttcatc gagtccgggc
      421 tggcgctggg ctttaccgcc gttttggcgc tgctggcggt gggtatctac gtcaccgcca
      481 agggcagcgt ggccagccgc tatgtcctga ccttcgttct ggtgggattt gtggcactgc
      541 acattcagct ggcgcgcggc atgatcgaat tccacttcgg tgtgttcgtg gtgctggctt
      601 tcctgctggt gtacctggac tggaagctca tcattttcgg ggcggccgcc tttgcggtgc
      661 accacatcgc atttgaccgc ctgcaggcag cgggtttggg gttcttctgc ctgacggagg
      721 ccaacttcgg gctgatcgtg gtgcacgccg tttatgtggt ggtgcagtcc gctgtagagg
      781 tggtgctggc gatcaatatg aaccgcgctg cccgccaggg cgatgaactc ggtgtactgg
      841 tgtccagcgt taaccgtgcc gatggcattt cgctggatgt gcgtggtgtg gccaccgaga
      901 ccgaaggggc caatgcgctc aaggccaccc tgagccgcat ggactcagct gtgtccaccg
      961 tgcgttccgg ggcgcagagc atcgaagtgg cctgctccga aattgccagc ggcaaccagg
     1021 acctcagcaa ccgcaccgag cagactgcgg ccaacctgca gcgcaccagc agcagcatgt
     1081 ccgcactcac cggcacggta cgccagtcgg ctgacagcgc gcgccaggcc aaccagctcg
     1141 ccatgaatgc cagcacggta gccgccaagg gcggcgacgt ggtggcccag gtggtggaga
     1201 ccatgcaggg catcaatgaa tccagccgca agatctcgga catcatcagc gtgatcgatg
     1261 gcattgcttt ccagaccaac atcctggcgc tgaatgcggc ggtcgaagcc gcgcgtgcgg
     1321 gcgaacaggg ccgtggcttt gccgtggtgg ccagtgaagt gcgctcgctg gccggccgtt
     1381 ctgccgaagc cgccaaagaa atcaaggccc tcatcggcgc cagcgtcgag cgcgtggagc
     1441 agggcacggc gctggtcggg caggcgggtg cgacgatgac cgaagtggtc accagcatcc
     1501 ggcaggtgac cgacatcatg ggtgaaatca gtgccgccac cggagagcag gctgcgggcg
     1561 ttgcccaggt cagccaggct gtgaccgaga tggaccaggc cacccagcaa aacgctgcac
     1621 tggtggagca gatggccgcc gcagccagca gtctcaagtc ccaggccgaa gagctggtgc
     1681 agactgtggc agtgtttggc gcagcggaag gcgcgcacca gactgcacgc cagttcagaa
     1741 gtgcgccagt gcaaaccgcg acaccacgtg tggccccagc ggcaaagcct ctcaatctct
     1801 ccggcagccg cccggctccg aaggctgcgc ccaaagccgg cgtgcccaag gtcggaatga
     1861 gcacgaaacc tgttgcgccg ccacagccgc cggctgtgcc caagatccag cccaagcccg
     1921 ccccggctgg cggtgatgag gagtgggaaa ccttttgacg gcacgtcctg aacaccatcg
     1981 cccaaggggg tttgccaaca ccgatcccga ggtcgtcatt ggcaatttcc cctggtacga
     2041 gatggtgtgg cgctcgctgc ggggcgactt caagccgctc tcggctccgg ccgaaggcta
     2101 cgcggcgttt gccaaagcgt ggtccacacc ggtcaaccat gcgcgccttg ctgttcgcca
     2161 gactgcaccg gtggtgacct ggctcggcca tgtgtccatg ctgctgcaag tcgcgggttt
     2221 gaacatcctg atcgacccga cgctggcgga ctgcgcggga ccattcggac gtgtgggtgc
     2281 gccgcgccgc gtgccggcac cgctcacgcc cgagcaattg ccgctgattg acgtggtact
     2341 gatttcgcac aaccactacg accacctgtg tgacgccact ctgtcgcggc tggtggcggc
     2401 tgggcagtca ccgcatttct atgtgccgct gggattgggg ccgtggttca tgtcacgcgg
     2461 catggaccgg gtgaccgagc tggactggtg gcaccaggcg ccgctgaagg agggggtgac
     2521 cctgcatttc acgccatcgc agcactggag ccggcgcacg ccgtgggaca cgaatgcgtc
     2581 gctgtggggt ggcttcatgg tggagtgggc gcggcagacg gagggcgagg ggggagaacc
     2641 ctggcgcttt ctttttcccg gagacaccgg ctacagcaac gatttcaaga ccatccgcga
     2701 gcgtttgggg gctgtggatt ttctggcgct acccattggc gcctacctgc cgcgcgactt
     2761 catgaagccc atgcatgtga accccgcgga cgcggtgcag ctcatgctcg acctggatgc
     2821 caaacaggcc atgggtgtgc actggggtac gtttctgctg acgcaggaag cgtttgacca
     2881 tccgccgcgc gatctggccg ctgccctgca cgaccgcggc ttgccgcagg agcgggtgtg
     2941 gctgatgaag cacggggaaa cgcgcgcgat ccccctgtag tcttctcttg cgcggccttc
     3001 aggccagcag caccgccagc atccagaaga acagcgcgag ctgcacgctc cagaaggtcg
     3061 cccgcaccag cacctgcaga acgcccacgc cggacaggtg cgccaggctc tggccaacgc
     3121 gggcatagaa cacccacggt gccagcgccg caatggcatc gagcttgccc agtgccgctg
     3181 cagccagaac gatgactgca aaaatcggca gattctccag actgttggcg tgggcgtctt
     3241 ccaggcgctt gaccagtggc gcatcgtccg cgggcttggc gccgcgcggc cagtggttga
     3301 tgggggtgcc ggtgacgaac cgcagaccgc ggtacaaaaa tacacctgta acgagcagca
     3361 gcgtccaggc agcgaatccg agcagggctt gtacagcggt catgggtttg tctccagcag
     3421 gttatgtttt ctatatacgg aacgttatgc agtccgtggc ttgactttgt tggcggccag
     3481 tttggcctgc gtgcgggcca cttccacatc ggcatcgtgc accagtgcca cgcccatgcg
     3541 gcgcttcaca aagctttcgg gtttgccaaa caggcggatc tcgctgttgg gcacgcgcat
     3601 ggcgtcatcc acgccgtcaa acacgatgcc ctgggcctcc acgccgccat agatcacggc
     3661 gctggcaccc gggctcttga gcgcggtgct gaccggcaga cccagaatgg cgcgcgcgtg
     3721 cagttcaaac tcgttctgcc actgcgtgat catggtcacc atgccggtgt cgtgcgggcg
     3781 tgggctcact tcgctgaacc acacgttgtc tcccttgacg aacagttcca caccgaaca
//
LOCUS       DAOSJM010000231         3836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_20924_length_3836_cov_3.780746, whole genome shotgun sequence.
ACCESSION   DAOSJM010000231 DAOSJM010000000
VERSION     DAOSJM010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20924_length_3836_cov_3.780746"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PKX82_08740"
     CDS             <1..271
                     /locus_tag="PKX82_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOF52380.1"
                     /translation="DEPTAALGVAETARVEQIIKGLKQQGIPLIIISHNLRQVFDLVD
                     RIWVFRQGRIICSRLTRQTSPEEIVGLITGAIDPARLQAEEAAAC"
     gene            265..705
                     /locus_tag="PKX82_08745"
     CDS             265..705
                     /locus_tag="PKX82_08745"
                     /inference="COORDINATES: protein motif:HMM:NF016885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOF52381.1"
                     /translation="MLKGIDPILTPELLMRLCAMGHGEWVAVVDANFTADFLSQGKPV
                     VRLPGLSLERVSRAVLSVFPLADDVQQPAAYMHACNHPMDHRTSAQQAIVDLVSEHGL
                     AQARVEPVERFAFYDKIKGASLIVQSGEATAYGNAMFCKGVILN"
     gene            723..1946
                     /locus_tag="PKX82_08750"
     CDS             723..1946
                     /locus_tag="PKX82_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOF52382.1"
                     /translation="MNPQPAFIRDPRQMRGSNHTGMRQFNERIVLQAIRHNGAIAKAD
                     IARLTQLSTQTVAIIVARLIDDGLLIKQDRVRGKIGQPSVPLSINPDGAYSLGLQVGR
                     RSLEVLVSDFLGQPRHRLQFHYPYPDPDVVLPNIQKGLADMQALMGDNWTRTVGIGLT
                     APLSMHRWSNLMGGAAGKALERWENIDLLAKVQEFTDLPVEFAKDTTAACIAEMLQGH
                     GRLVDNFLYVFVGTFVGGGLVMSGHIMAGQRSNAGAIGSLPMGMGSASDKPPQLLQLA
                     SGWQLEQTLEQAGFDAQLVHQDAIMDPAYAAFVTPWLTQAGQALAMTAVSATALMDLD
                     AVIIDGSLGRTLIDALIRATRERMQHYNLDGLQPPTVMAGTVGAHARALGGSLLPLHT
                     QFFPIKDIFLKEDLA"
     gene            1943..2614
                     /locus_tag="PKX82_08755"
     CDS             1943..2614
                     /locus_tag="PKX82_08755"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOF52383.1"
                     /translation="MKLYLDSAQVSTWALPPGCPPVCGVTTNPTLVHQAGLRVSLDSY
                     EALIRAAADHGMAEVMLQLPSPDATQARAWQERLHQLSTNAGIALTIKLPCHPDWLPC
                     IAEMQRAQQAFLLTGLSNAVQLLWAQSLGATWVAPYVGRLATDGRDVWPLMEACVAGQ
                     DHGPGLLAASIKSADVLARLMACGAAAATLPPASLVAWSGDALTLAAIAQFERDTAAS
                     LHQAR"
     gene            complement(2622..3644)
                     /gene="waaF"
                     /locus_tag="PKX82_08760"
     CDS             complement(2622..3644)
                     /gene="waaF"
                     /locus_tag="PKX82_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOF52384.1"
                     /translation="MASTSPVGTESFVIAPQWIGDAVMTEPLLRRLHARGEQLTVGAL
                     PWVAPVYRAMPQVSKVIEFPFAHGGLQWSARRALAQQLRGRFDTAYICPNSLKSALLP
                     WWAHIARRVGYRGESRTLLLTQALPNPTKGARPPMVAFYSALSGEAGLETDRPSLHME
                     PAAADAALTALSLQRGAYYVFAPGAEYGPAKRWPAAHFAALAQQLPLPVVLLGSSKES
                     GLCREIATAANATRPGQCLDFSGKTSLDQALAVIAAARAMVSNDSGLMHVAAAFGVPQ
                     VALFGSSSPLHTPPLSDRAHVIWLKNDTSYQPALDCAPCFARTCPLGHTRCLVDITPE
                     QVLRYL"
     gene            complement(3616..3822)
                     /locus_tag="PKX82_08765"
     CDS             complement(3616..3822)
                     /locus_tag="PKX82_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HOF52385.1"
                     /translation="MSTSVIELLAQDLNHQGGVFCPSPLAKMETWNTHPKVYLDVAHT
                     GEAKCPYCGTVYKLKDGEHFAGGH"
BASE COUNT          727 a         1265 c         1199 g          645 t
ORIGIN      
        1 ggacgagccc accgccgcat tgggcgtggc cgagaccgcc cgcgtggagc agatcatcaa
       61 gggactcaag cagcagggca tcccgctgat catcatcagc cacaacctgc gccaggtgtt
      121 tgatctggtg gaccgcatct gggtattccg ccagggtcgc atcatctgca gccgcctgac
      181 ccggcaaacc agccccgaag aaatcgtggg cctgatcacc ggcgccattg atccggccag
      241 actgcaggcc gaggaggcag cagcatgctg aaaggaatcg acccgatcct gacgcccgag
      301 ttgctgatgc gcctgtgcgc catgggccat ggcgaatggg tggccgtggt ggacgccaac
      361 ttcactgccg atttcctgag ccagggcaag cccgtggtgc gactacccgg cctgagtctg
      421 gagcgcgtgt cgcgggccgt cctttccgtg tttccgctgg cggacgacgt gcaacaacct
      481 gcagcctaca tgcacgcatg caaccacccg atggaccacc gcacatccgc ccagcaggcc
      541 atcgtggatc tggtgtcgga gcacggcctg gcgcaagcac gcgtggagcc ggtggagcgc
      601 tttgcgtttt atgacaagat caagggtgca agcctgattg tgcagagcgg cgaggccacg
      661 gcgtatggca atgccatgtt ctgcaaaggc gtcatcctga actaacccta cggccatccc
      721 tcttgaaccc ccaacctgct tttatccggg acccgcgcca gatgcggggg tccaaccaca
      781 ccgggatgcg ccagttcaac gagcgcatcg tgttgcaggc catccgccac aacggcgcca
      841 tcgccaaggc cgatatcgcc cgcctgacac agctctccac ccagacggtg gccatcatcg
      901 tcgcgcgcct gatcgatgac ggcctgctga tcaagcagga ccgggtgcgc ggaaaaatcg
      961 gacaaccgtc cgtacccctg tcgatcaatc ccgatggggc ctacagcctg ggcctgcagg
     1021 tggggcgtcg cagcctggaa gtgctggtgt cggactttct ggggcagccc cgccaccgcc
     1081 tgcaattcca ttacccctac cccgacccgg acgtggttct gcccaacatc cagaagggtc
     1141 tggcagacat gcaggcgctg atgggcgaca actggacacg caccgtgggc atcggactga
     1201 ctgcgccgct gtcgatgcac cgctggtcca acctgatggg cggcgcggct ggaaaagcgc
     1261 tggaacgctg ggagaacatt gacctgctgg ccaaggtgca ggaattcacg gacctgcccg
     1321 tcgagtttgc caaagacacc acggccgcct gcattgccga gatgctgcaa gggcatgggc
     1381 gcctggtgga caacttcctc tacgtgtttg tcggcacctt tgtgggcggc gggctggtca
     1441 tgtccggaca catcatggcc gggcagcggt ccaatgcggg cgccatcggg tcacttccca
     1501 tgggcatggg cagtgcatcg gacaagcccc cccaattgct gcaactggcc tccggctggc
     1561 aactcgaaca gacactggaa caggcgggat tcgacgccca gctggtgcac caggacgcca
     1621 tcatggaccc ggcctacgcc gcctttgtca cgccctggct gacccaggcc gggcaggcgc
     1681 tggccatgac tgccgtcagc gcgactgcac tgatggatct ggacgcagtg atcatcgacg
     1741 gctccctggg tcgcacgctc attgatgcgc tgatccgcgc aacacgtgaa cgcatgcagc
     1801 actacaacct ggacggtttg caaccaccca ccgtcatggc gggaacggtg ggtgcccatg
     1861 cacgggcact gggtggctcg ctgctgcccc tgcacaccca atttttcccc atcaaggaca
     1921 tctttctcaa ggaagatctg gcatgaagct gtatctcgat tctgcgcagg tcagcacctg
     1981 ggcactgcca ccgggctgcc cgcccgtgtg tggtgtcacc accaatccca cattggtcca
     2041 ccaggcgggg ttgcgcgtct ccctggattc ctacgaagcc ctgattcgtg ccgcggccga
     2101 ccacggcatg gctgaggtga tgctgcaatt gcccagcccc gatgccacac aggcacgcgc
     2161 ctggcaggag cgcctgcacc agctgtcgac caacgcaggg attgcactca ccatcaaact
     2221 gccctgccac cccgattggc tgccctgcat cgccgagatg caacgggcac agcaggcctt
     2281 cctgctcacc ggattgtcca acgcggtaca gctgctgtgg gcgcagtccc tgggagctac
     2341 ctgggttgcg ccgtatgtgg ggcgccttgc aacagatgga cgggacgtat ggcccctgat
     2401 ggaagcctgc gtggcggggc aggaccacgg cccgggcctg ctggcggcga gcatcaaaag
     2461 tgccgacgta cttgcgcgat tgatggcctg tggcgccgcc gcagcgacgt tgccgcctgc
     2521 cagcctggtg gcctggtcgg gcgacgcact gacgctcgct gcaattgccc aattcgaacg
     2581 cgacactgca gccagcctgc accaggcgcg ctaacccgcg atcacaaata ccgcaggacc
     2641 tgttccggtg tgatgtccac caggcaccgc gtatggccca gcgggcaggt gcgtgcaaag
     2701 cacggagcgc aatccagcgc aggctggtag ctggtgtcat tcttgagcca aatcacatgg
     2761 gcccggtcgc tcaaaggcgg ggtgtgcagt gggctggacg aaccaaacag cgccacttgc
     2821 ggtacgccaa aagccgcggc cacatgcatc agcccggaat cgttgctcac catggcacgc
     2881 gctgcggcaa tcacggcgag cgcctggtcc agtgaggtct tgccggagaa atccagacac
     2941 tgcccaggac gcgtggcatt cgctgcggtg gcgatctcgc ggcacagtcc ggactccttg
     3001 gacgacccca gcaacaccac cggcaaaggc agctgctgcg ccagtgcggc aaagtgggcc
     3061 gccggccagc gcttggccgg accgtactcg gcccccggcg cgaacacgta ataagcaccg
     3121 cgctgcagcg acagagccgt caacgcagcg tctgccgctg ctggttccat gtgcagggag
     3181 ggacggtccg tctccagccc ggcctcgcca ctcaacgccg aataaaacgc caccatgggc
     3241 ggccgggcac ctttggtggg attgggcaac gcttgtgtga ggagcagcgt gcgcgactca
     3301 ccccggtacc ccacccggcg ggcaatatgg gcccaccagg gcaacagtgc actcttgagg
     3361 gaattgggac aaatgtaggc agtatcaaag cggccgcgca actgctgcgc cagggcacgt
     3421 cgcgcgctcc actgcagccc gccgtgggca aagggaaact cgatgacctt ggacacctgc
     3481 ggcatggccc ggtacaccgg tgccacccag ggcaatgcac ccaccgtcag ctgttcaccg
     3541 cgcgcgtgca gacggcgcaa cagcggttcc gtcatcaccg cgtccccaat ccattggggc
     3601 gcgatgacga aagactcagt gcccaccggc gaagtgctcg ccatccttca gtttgtacac
     3661 cgtgccgcaa taggggcact tggcttcgcc ggtgtgggcg acatccaggt acaccttggg
     3721 gtgggtgttc caggtttcca ttttggccag cggactgggg cagaacacgc caccttggtg
     3781 gttgaggtct tgggccagca attcaatgac agaggtactc atatgcgttc tcggtg
//
LOCUS       DAOSJM010000232         3829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_20973_length_3829_cov_5.293058, whole genome shotgun sequence.
ACCESSION   DAOSJM010000232 DAOSJM010000000
VERSION     DAOSJM010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20973_length_3829_cov_5.293058"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1261
                     /locus_tag="PKX82_08770"
     CDS             209..1261
                     /locus_tag="PKX82_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOF52386.1"
                     /translation="MTRLRLRSLLLCLCALLCSVAPASQAADVLRVLAWPGYADPDIV
                     KTFEQRTGSKVEVIYIDSDEALWQRVHKNKSGDFDVFAVNTAELQRYIQTSLVRPIDT
                     RALPNLTRQLPRFRDLKGIPGIVHSGGVFAIPYTYSEMGLIYHREQIKTPPNSIKILW
                     DPKYRGKVLLYNAGSHSFSLAAQALADTTPFDIKATEWPAAVQQLIALRRNAMAFYLQ
                     PDESVDLFKRGKAALMFANYGSQQVKLLQQAGLNVGYAIPQEGALAWLDCWVVTQGAR
                     NTALAHSWINYMLEATPGAVLVSRQGLSNTTSESPFLRSQDRLIWLEPVEDVVRRTQL
                     WERVMSGDRASKVLAP"
     gene            1258..2994
                     /locus_tag="PKX82_08775"
     CDS             1258..2994
                     /locus_tag="PKX82_08775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOF52387.1"
                     /translation="MKFGITARLAVVLALIGMAVSGLTGFYAYSVSRDLLIQSAQGEL
                     MTSTQVVARRISQTRQELSRNLMLLAAQPEALVALKAQCARSSSSARLALEFRELMRV
                     NPSYFQLRLISAAEGGPECVRVDRDANALLEISGDDLQEKGHFMYVTEALKLKPGQTY
                     LSQITINHERGAHAGQEKPTVILSTPVVDAQGKAVGVIVINVDLNGMFSLLAADLPGD
                     HRLYLSNHRGDFLIHPDTTRTFGFDRGQRVLLQDEFPQTVDVVAGKDKSVLLETQANA
                     EGTPPLIAAFIASNVVVPGMEERLLLGLAQPRDVVLQQADRLGLGVLQIVLVVSLACV
                     ALALLVARAIARPINAMNAAVQKFSNDTQEMALPVDRQDEIGMLARSFARMQTVIRRQ
                     MHDLQDNQEELEHLAQHDMLTDLPNRRMLMDRLDNAIARAKRSEVGFALLFVDVDRFK
                     SFNDRYGHAAGDAVLKAVADRLRHGIRAVDTAARLGGDEFVLLLDQFVHRDQIAGFTE
                     KLLALLKAPIDFQGQTLQAEFSIGISQFPDDGSTAEDIVANADRAMYRTKTSGRNGYR
                     FTSDDTTQSSLL"
     gene            3025..3120
                     /gene="ffs"
                     /locus_tag="PKX82_08780"
     ncRNA           3025..3120
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PKX82_08780"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(3128..3370)
                     /locus_tag="PKX82_08785"
     CDS             complement(3128..3370)
                     /locus_tag="PKX82_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOF52388.1"
                     /translation="MKSDLARVDAHVVAKDEYDELPEADDAMLARAQVNRGGRPVAAN
                     PRKLLSIRLPADVIDRWKATGPGWQTRMAERLSSVR"
     gene            complement(3360..3635)
                     /locus_tag="PKX82_08790"
     CDS             complement(3360..3635)
                     /locus_tag="PKX82_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOF52389.1"
                     /translation="MRITFDPAKRDKALAERGLDFLDAEIVFEGVTLEFEDTRKEYGE
                     QRIICYGLLAGRMVVVGYTPRGADRHIFSMRKANEREQTRIAQLLEV"
BASE COUNT          794 a         1193 c         1100 g          742 t
ORIGIN      
        1 aacaggtagg cgtggtgcag gcgctgggtg gtcagcgcat tggtgagggc ttgcaccaca
       61 tggtcctgcc ccaccatttc ggtgaaattg cgcggacggt acttgcgggc gagcacgagg
      121 taagacatgc acaggattct agtgcgctaa ctctgggcgt ctcggttaga ctgatacaaa
      181 ttgatacaac accctgtcgg acaccttgat gacccgcttg cgcctgcgta gcctcctgct
      241 ctgcctttgc gccctgctgt gctcggtggc ccctgcatcc caggcagcgg acgtgttgcg
      301 tgtactggca tggcctggtt atgccgaccc ggatatcgtc aagaccttcg agcagcgcac
      361 gggcagcaag gtggaagtca tctacatcga ctccgacgaa gccctgtggc agcgggtcca
      421 caaaaacaag tcgggggatt ttgacgtttt tgccgtcaac actgcagaac tgcagcgcta
      481 tatccagacc tcgctggtgc gccccatcga cacccgggcc cttcccaatt tgacccgcca
      541 gcttccgcgc tttcgcgacc tgaaggggat tccgggcatc gtccacagtg gcggtgtgtt
      601 tgccattccc tacacctatt cggagatggg cctgatctac caccgcgagc agatcaagac
      661 gcccccgaac tccatcaaga tactgtggga tccgaaatac cgggggaaag tgctgctgta
      721 caacgcagga tcgcacagct tctccctggc agcacaggcg ctggcggaca ccacgccgtt
      781 tgacatcaaa gcgaccgagt ggcccgccgc agtgcagcaa ctcattgcgc tgcgccgcaa
      841 tgccatggcg ttctatctgc agccggatga gtcggtagac ctgttcaagc ggggcaaagc
      901 cgcgctgatg tttgccaact acggatcgca gcaggtcaaa ctcctacagc aggctggact
      961 gaatgtgggc tacgccatcc cgcaggaggg ggcactggca tggctcgact gctgggtggt
     1021 cacccagggg gcgcgcaaca ccgcgctggc acacagctgg atcaactaca tgctggaggc
     1081 cacaccgggc gctgttctgg tgagtcgcca gggtttgtcc aacaccacct cggaatctcc
     1141 ctttcttcgg tctcaggacc gcctgatctg gctggaaccg gttgaggacg tggtgcgccg
     1201 cacccagctg tgggagcggg tgatgtcggg tgaccgggcc agcaaggtac ttgcaccgtg
     1261 aaattcggca ttaccgcacg cctggctgtg gtgttggcac tgataggaat ggccgtgtcc
     1321 ggcctgaccg gtttttacgc ctacagtgtc agccgcgacc tgctgatcca gtcagcgcag
     1381 ggagagttga tgacatccac ccaggtcgtg gccaggcgta tcagccagac ccgacaggaa
     1441 ctgtcccgca acctgatgct gctggcagca caacccgaag cgctggtggc cctcaaggcg
     1501 caatgtgcgc gctccagttc cagcgcaaga ctcgcgctgg aattcaggga gctgatgcgc
     1561 gtcaacccct cttattttca gctcaggctt atttctgcgg cagaaggtgg cccggagtgt
     1621 gttcgtgtgg accgcgacgc caacgcgctg ctggaaattt cgggggacga cctgcaggag
     1681 aaggggcact tcatgtatgt caccgaagcc ctcaagctca agccgggaca aacctatttg
     1741 tcccagatca ccatcaacca tgagcggggc gcccacgcgg gccaggaaaa acccaccgtg
     1801 atcctgtcca ccccggtggt ggatgcccag ggtaaggcgg tcggtgtcat cgtcatcaac
     1861 gtggatctca atggcatgtt cagtctgctg gctgccgatc tgcctgggga ccacagactt
     1921 tacctgtcca accaccgggg tgattttctg atccatcccg acaccacacg cacatttgga
     1981 tttgaccgtg gccagcgcgt gctgctacag gatgaattcc cgcaaaccgt ggacgtcgtt
     2041 gccggcaagg acaaatccgt actgctggaa acgcaagcga acgctgaagg cacgcccccc
     2101 ctgattgcag cctttatcgc cagcaacgtg gtcgtccccg gcatggaaga aaggctgctg
     2161 ctcgggctgg cacaaccccg cgatgtcgtg ctgcagcaag ccgaccgcct gggcctcggc
     2221 gtgctgcaaa tcgttctggt cgtgagtctg gcctgtgtgg cgctggcact gctggtagcg
     2281 cgcgccatcg cccgccccat caacgccatg aatgcagcgg tccagaagtt ttccaacgac
     2341 acccaggaaa tggccttgcc cgtcgatcgg caggacgaaa ttggcatgct ggcgcgcagc
     2401 tttgcacgca tgcaaaccgt catccgccgg cagatgcatg acctgcagga caaccaggaa
     2461 gaactggaac accttgcgca gcacgacatg ctgaccgatc tgcccaaccg gcgcatgctc
     2521 atggaccgcc tggacaatgc gattgcccgg gccaaacgga gcgaagtcgg ttttgcattg
     2581 ctgttcgtcg acgtggaccg gttcaaatcc ttcaacgacc gttacggtca tgccgcgggc
     2641 gatgccgtac tgaaggccgt tgccgatcgg ctgcggcacg gcatccgtgc cgtcgacacg
     2701 gcagcacgcc tgggtgggga cgaatttgta ttgctgctgg accagttcgt tcacagggac
     2761 cagattgccg gatttaccga aaaactgctc gccctcttga aagcccccat cgacttccag
     2821 gggcagacac tccaggcgga attcagcatt ggcatcagcc agttccctga cgatggaagc
     2881 acggcagagg acatcgtggc caacgccgac cgggccatgt accgcaccaa gaccagtggt
     2941 cgcaacggct accgtttcac ttccgacgac accacccagt ccagcctgct ctagatcgca
     3001 ctgcgattgg gtacaatccg cagtgacggg ccctctcgca tggtgaagcg gccaaccggg
     3061 tcaggtgggg aaccaagcag ccctaactgt ggagccagtg ccgggatcag gctcgtcaac
     3121 taatatttta ccgaacggaa ctcagccgtt ctgccatcct tgtttgccag cccgggccag
     3181 tggccttcca ccggtcaatc acgtccgctg gtagccggat agacaacagc ttgcgcggat
     3241 tcgccgccac aggacgcccg ccccggttta cctgtgccct ggcaagcatt gcatcatccg
     3301 cttcgggaag ttcgtcgtac tcatccttcg ccacgacgtg tgcatccacc cgggccagat
     3361 cagacttcaa ggagctgggc aatgcgggtt tgttcgcgct cattggcttt cctcatactg
     3421 aagatgtggc gatccgcgcc acgcggtgtg tatcccacaa caaccattcg cccggccagc
     3481 aacccgtagc aaataatgcg ctgctcgccg tactctttgc gcgtgtcttc gaattcaagg
     3541 gtaacgccct caaagacgat ctcggcgtcc aaaaagtcca gccctcgttc cgccaaagcc
     3601 ttgtcacgct tggctggatc gaatgtgata cgcataagtt attgtagata caaaaactta
     3661 gtactacaag aagacaaatt agtgttcacc atcaagcaca agtaaccggg ctcaataccc
     3721 gcccatccct caatcgggaa gccagcatca ccccgaagtc tgcaaatacg tacacgcgcc
     3781 gtggcccgca cgccacccac tggcaaagct ggcctgcaac aggtagccg
//
LOCUS       DAOSJM010000233         3817 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21054_length_3817_cov_4.535619, whole genome shotgun sequence.
ACCESSION   DAOSJM010000233 DAOSJM010000000
VERSION     DAOSJM010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21054_length_3817_cov_4.535619"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PKX82_08795"
     CDS             <1..471
                     /locus_tag="PKX82_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HOF52390.1"
                     /translation="PHAERRAVSSNAEGARLAAGNPAWAGIASERAGSEFGLHIAAHA
                     IQDEAFNRTRFVVVCLPSVLPAPEASGKDCTSLVVSVPNRPGAVHDMLVPLKMHGVSM
                     TRFESRPARSGQWEYYFYIDLQGHPSQPHVAAALKDLQSLCAFYKVLGTYPLAD"
     gene            493..1368
                     /locus_tag="PKX82_08800"
     CDS             493..1368
                     /locus_tag="PKX82_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOF52391.1"
                     /translation="MFEQLGLIGCGLMGGSFALALKRAGLVKRVVGYSKSPSTTERAR
                     QMGVIDIEAPSALLAVSGADIVLIAVPVAATEATFKAIKHLVTPNMLIMDVGSTKRDV
                     IDAARRALREHVGLFVPAHPIAGKELSGVEHADADLYAGRQVILTPIERTQTIQLQKA
                     VDLWTALGCRVLKMSPEQHDSAFAAVSHLPHLIAFALMNSIAGQPQGKDYLSLAGPGF
                     RDFTRIAASDPKVWRDIMVSNREELLAQSKIFQRNLQALELMITSGNSEALEGLIQQA
                     SDTRANWSMASKLNK"
     gene            1368..3374
                     /locus_tag="PKX82_08805"
     CDS             1368..3374
                     /locus_tag="PKX82_08805"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HOF52392.1"
                     /translation="MFSTAFLDLPALAGAHGEVSLPGSKSISNRVLLLAAMCSGTTII
                     HDLLDSDDTRVMLVALKALGCTIEQTGATVRITGVAGRLPTEKHPLFLGNAGTAMRPL
                     TAALAVLGGDFELSGIPRMHERPIGDLVDALRQLGCQIDYLGNDGFPPLRIGQAHLNL
                     GAPIQVRGDVSSQFLTALLMALPLVAQRDIVIDVVGELISRPYIEITLNLLERFGVQV
                     LREGWQRFTIPAGSQLRSPGELHVEADASSASYFIALGAIATGASTQNGIKILGVGAD
                     SIQGDIRFMEAAQAMGARIESGPNWLRIQRGDWPLKAIDLDCNHIPDAAMTLAVMALY
                     ADGTTTLRNIASWRVKETDRIAAMATELRKLGATVVEGADLIQVTPPATPSDWKAASI
                     HTYDDHRVAMCFSLAAFNPAKLPVRIEDPKCVAKTFPDYFEALFSVAQAQPDAVPVIC
                     VDGPTASGKGTLAAVLAEQLGYHFLDSGSLYRITGLAATRAGIALDVANEQAIVALLQ
                     SLPIHFEGSHIWLANEDVSEAIRTEEAGMNASRVSAFPAVRAALIDLQRGFRKLPGLV
                     ADGRDMGTMIFPQAPLKVFLTASAACRAERRYKQLISKGISATLDSLRADLEARDARD
                     MSRSVAPLKPAQDAKLLDNSDLTVETSVELVLDWWQSKQPFSSR"
     gene            3506..>3817
                     /locus_tag="PKX82_08810"
     CDS             3506..>3817
                     /locus_tag="PKX82_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOF52393.1"
                     /translation="MSESFAALFEESLQRSEMRTGEVITAEVVRIEHSFVVVNAGLKS
                     EAYVPIEEFKSDKGELEVQVGDFVSVAIDSVENGYGDTILSRDKAKRLASWMSLEKAL
                     ES"
BASE COUNT          722 a         1274 c         1136 g          685 t
ORIGIN      
        1 ccccacgccg agcgccgcgc cgtctccagc aatgctgaag gcgcccgctt ggccgccggc
       61 aacccggcct gggctggcat tgccagcgag cgcgcgggtt ccgagtttgg cctgcatatt
      121 gcggcgcacg cgatccagga cgaagccttc aaccgcaccc gctttgtggt ggtgtgcctg
      181 cccagtgtgc tgcccgcacc cgaggcgtca ggcaaggact gcaccagcct ggtggtgtcg
      241 gtgcctaacc ggcccggcgc ggtgcacgac atgctggttc cgttaaaaat gcacggagtc
      301 tccatgacgc ggttcgagtc gcgtcccgca cgctccggcc aatgggagta ctatttttat
      361 atcgacctgc agggccaccc ctcgcagccc catgtggcgg ccgccctgaa agacctgcag
      421 agtctgtgcg ccttttacaa ggtgctgggt acgtacccgt tggccgattg acacgcttgc
      481 acaggaactc atatgtttga acagctaggt ttgatcggct gcggcctgat gggcggttcg
      541 tttgccttgg ccctcaagcg cgcgggtctg gtcaagcggg tggtgggcta cagcaagtca
      601 cccagtacca ccgagcgggc acgccagatg ggcgtgattg acatcgaagc accctccgca
      661 ctgctggccg tctccggggc cgacatcgtg ctgatcgcgg tgccggtcgc cgccaccgaa
      721 gccaccttca aggccatcaa gcacctggtc acgcccaaca tgctgatcat ggacgtgggc
      781 tccaccaaac gcgacgtgat cgacgcggcc cgccgtgcgt tgcgcgagca tgtcggcctg
      841 ttcgtacccg cccacccgat cgcgggcaag gaactctcgg gcgtggagca cgccgacgcc
      901 gacctgtacg ccgggcgcca ggtcatcctg acgccgattg agcgcaccca gaccatccag
      961 ttgcagaaag cggtcgacct ctggaccgcg ctgggctgcc gcgtcctgaa aatgtcgccc
     1021 gaacagcatg actctgcgtt cgcggccgtc agccacctgc cccacctgat tgcgtttgcc
     1081 ctgatgaact cgatcgcagg ccaaccgcaa ggcaaggact acctgtcgct ggcgggtccg
     1141 ggctttcgcg atttcacccg catcgccgcc agtgacccca aggtttggcg cgacatcatg
     1201 gtctccaacc gcgaagagct gctggcacag agcaagatat tccagcgcaa cctccaggcg
     1261 ctggaactca tgattaccag tggcaactcc gaggcgctcg aagggctgat ccagcaggcc
     1321 agcgacaccc gtgccaactg gagcatggcc tccaaactca acaaataatg ttttccactg
     1381 catttctgga cctgccggca ctggcaggtg cccatggcga ggtcagcctc ccgggctcca
     1441 aaagcatttc caaccgcgtg ctcctgctgg ccgccatgtg cagcggcacc accatcatcc
     1501 acgacctgct ggactccgat gacacccgcg tcatgctggt ggcattgaag gcgctgggat
     1561 gcaccatcga gcagacgggc gcaaccgtgc gtatcaccgg cgtggccggg cgcctgccca
     1621 ctgagaaaca cccgctcttt ctgggcaacg caggcaccgc catgcgcccc ctgacagcag
     1681 cactcgccgt gctcggcggt gactttgaac tcagcggcat cccccgcatg cacgagcgcc
     1741 ccatcggtga tctggtggat gcgctgcgcc agctgggttg ccagattgac tacctgggca
     1801 acgatggctt tccgcccctg cgcatcggcc aggcccacct gaacctgggc gcacccatcc
     1861 aggtgcgcgg tgatgtgtcc agccagttct tgaccgcgct gctgatggcc ctgcccctgg
     1921 tggcgcagcg tgacatcgtg attgacgtgg tgggcgaact gatctcccgc ccgtatatcg
     1981 agatcacgct gaacctgctg gagcgctttg gtgtgcaggt gctgcgtgag ggttggcaac
     2041 gcttcaccat ccccgcaggc agccagttgc gttcgcccgg cgaactgcat gtcgaggccg
     2101 acgcctcatc tgctagctat ttcatagcgc ttggcgcaat agcgacgggg gctagcactc
     2161 aaaatggcat aaaaatcctg ggcgtcggtg ccgattccat ccagggcgac atccgcttca
     2221 tggaagcagc ccaggccatg ggtgcccgca tcgagagcgg ccccaactgg ctgcgcatcc
     2281 agcgcggcga ctggccgctc aaagcgattg acctcgattg caaccacatc cccgacgcgg
     2341 ccatgacgct ggccgtgatg gcgctgtacg ccgatggcac caccaccctg cgcaacatcg
     2401 cgagctggcg ggtcaaggaa accgaccgca tcgccgccat ggccaccgag ctgcgcaagc
     2461 tcggcgccac cgtggtggaa ggcgccgact tgatccaggt gactcccccg gcaacgccca
     2521 gcgactggaa ggccgccagc atccacacct acgacgacca ccgcgtggcc atgtgcttct
     2581 cgctggccgc attcaacccg gccaaattgc cggtgcgcat cgaagaccca aaatgcgtcg
     2641 ccaagacctt tcccgattat tttgaagcgc tgttctcggt ggcgcaggcc cagcccgacg
     2701 ccgttccggt gatctgtgtg gacggcccca cggcatccgg caaaggaacg ctggcggccg
     2761 tactggccga gcaactgggc taccacttcc tcgattccgg gtcgctctac cgcatcaccg
     2821 gactggcggc cacacgtgcc ggcattgcgc tggacgtggc caacgagcag gccatcgtcg
     2881 cattgctgca aagcctgccc attcactttg aaggtagcca catctggctc gccaacgaag
     2941 acgtgagcga ggccatccgc accgaagagg cgggcatgaa tgcctcccgg gtgtcggcat
     3001 tcccggccgt ccgggccgcc ctgatcgacc tgcaacgcgg atttcgcaag ttgcccggcc
     3061 tggtcgccga cggccgcgat atgggcacca tgatcttccc gcaggccccg ctcaaggtct
     3121 ttctgactgc cagcgccgcc tgcagggcgg agcgccgcta taagcagttg atttcaaagg
     3181 gaatttcagc tacactcgac tctcttcgcg cggatctgga agcccgcgat gcccgggaca
     3241 tgtctcgcag cgtcgcacct ttgaaacctg cgcaggatgc caagttgctc gacaactcgg
     3301 atttgacggt cgaaacgtcc gttgaactcg tactggactg gtggcaaagc aaacagcctt
     3361 tcagctcgcg ctgaagggtt ggacagaagc aaggcctcac ggctttgcgt ggtccccacg
     3421 gctctcttcg cgcgacagcg caccggttca caccaagggc aatggggttc gataacttaa
     3481 ccccgcggtg caaaccgcaa cttaaatgtc agaatctttc gcagccctat ttgaagaatc
     3541 cctgcaacgc tcggaaatgc gtacaggtga agtgatcacc gctgaagtcg ttcgtatcga
     3601 gcacagcttc gtcgtggtga acgccggact gaagtccgaa gcctacgtgc caatcgaaga
     3661 gttcaagagc gacaagggcg aactcgaagt gcaagttggc gacttcgtgt ccgtggccat
     3721 cgactccgtg gaaaacggct atggcgacac catcctgtcc cgcgacaaag ccaagcgtct
     3781 ggcttcctgg atgagcctgg aaaaggccct ggaatcc
//
LOCUS       DAOSJM010000234         3801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21161_length_3801_cov_3.031767, whole genome shotgun sequence.
ACCESSION   DAOSJM010000234 DAOSJM010000000
VERSION     DAOSJM010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21161_length_3801_cov_3.031767"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="ugpQ"
                     /locus_tag="PKX82_08815"
     CDS             <1..715
                     /gene="ugpQ"
                     /locus_tag="PKX82_08815"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HOF52394.1"
                     /translation="KLAPENTLAAFRLGAQYGFRMFECDVKLSSDGVPFLLHDATLQR
                     TTNARRKIGPGNSAIAGDHPWGTLAQLDAGGWHSRSFAGEPLPTLDAVAAYCIANGYL
                     LNIEIKPTPGSERHTGEIVAQHVARLWQGQPVPPLLTSFQVEALQGAMETQPELPRGL
                     LLDTLWTGWLETALTLGCQAIVCNHALWDRSSVSQAQSAGFRTLSYTVNDEWAAQRLI
                     DLGADGIITDRVDLFSPAG"
     gene            complement(731..1984)
                     /locus_tag="PKX82_08820"
     CDS             complement(731..1984)
                     /locus_tag="PKX82_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52395.1"
                     /translation="MSASGYFDSPWPAEDGGPQRLQMASGGGEIALEPGDKLGCVTRN
                     TLLSTMTVLGAPGEVFLLTHSALRARLGLPTTSCVERIDPLTLQTLERSPRLPGGPMW
                     PGGMAVHRNGQLVVVYGRYAHTLSRNCSLLKSRELPVNAPYNSFVVLDNGLVVTKNLS
                     ETENAYLTVLDPDSLAPVCSETECPEPSVARLSAVGNTVYVVGVRTIFRFHWNDLTMR
                     LERDCDWFFDYTHQTRQTFGWDVVLDGQNAWFMDNGKHRYLYTMLGAGVSKTSNRLIR
                     VSMQDSTDTSVRTVSGLPAGSVTNPPLFDIDRSIVVAYDSANAYLAAWRFDTALRAFT
                     LLWTKKKFGCASHMVLFRQTGQIAVNDYRERGEEVVVLDIETGAELGRVRTGGITQGV
                     VFPSVGWGRDFYWSSMGRLARVFVA"
     gene            complement(1981..3756)
                     /locus_tag="PKX82_08825"
     CDS             complement(1981..3756)
                     /locus_tag="PKX82_08825"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HOF52396.1"
                     /translation="MSRIPFALWLLLSVVGSALPAGAAEPKRPNIVVMVADDWGFSDV
                     GSFGSEIATPNLDALARKGMRFSNFHVAAECSPTRAMLMTGVDSHRTGVGAMRESVPR
                     SHYGKPGYLTVLNQNVVTVSSLLQDGGYRTYAVGKWHVGKEPYNLPNARGFDRSLVQG
                     DSGSDNWETDKRYMALTDKVYWFENGKEVAMPKDYYSSEYYVSRTIDYLRQDATSAKP
                     FYAYLAFQANHIPVQAPREFIDKYRGVYKDGWTALRKARRDRAAALGLVPKDAPMVTM
                     PTTTDWDALSPEQKQYEVRRMEVYAGMADAMDHHVGRLVAYLKESGQYDNTVFVFLSD
                     NGSEASDPYAITSARLWLATEYTNDLEKLGDKGAYGTIGPSWASASASPLSTYKFYSG
                     EGGVRTPLIVSGVPGMRSDAVYPKFAHVKDIAPTLLDLAGVQHPGTQYKGQSVEAMTG
                     RSLMPVLKSTDVRAHPVDEPVGYELSGNMAVYRGDLKLTRNMAPVGDGQWHLYDIVKD
                     PGETQDLQSSQPDAFQRMRADYDAYAQANGVLPMPEGYEPRNQVLINSLVNVYLPRAR
                     IPAAVALVVLVGLVVYRRRRHRVQP"
BASE COUNT          742 a         1225 c         1118 g          716 t
ORIGIN      
        1 caagctggcg cccgaaaata cgcttgcagc gttccggctg ggagcgcagt acggctttcg
       61 catgttcgag tgcgacgtca agctcagcag cgatggggtt ccctttttgc tgcacgacgc
      121 caccttgcaa cgcacgacaa atgcacgcag gaaaatcggc cccggcaaca gtgcgattgc
      181 cggtgaccac ccgtggggca cgctggcgca actggacgcc ggtggctggc attcgcgcag
      241 ctttgccgga gagcccttgc ccactctgga cgctgtggcg gcctactgca ttgccaatgg
      301 atacctgctc aacatcgaga tcaagccgac acccggtagc gaacgccaca ccggggaaat
      361 cgtggcgcag catgtggccc ggttgtggca gggtcagccc gtccccccct tgctgacatc
      421 cttccaggtg gaagcgctgc aaggtgcaat ggagacacaa ccggaattgc cccgtgggct
      481 gctgctcgat acgctgtgga ccggctggct ggaaaccgct ctgacactgg gctgccaggc
      541 gatcgtctgc aaccacgcat tgtgggatcg cagcagtgtg tcccaggcac agagcgccgg
      601 tttcaggacc ctgagctaca ccgtcaatga cgagtgggcg gcccagcggc tgatcgatct
      661 gggcgccgat ggcatcatca ccgaccgggt cgacctgttc agccccgcag gctgatgctt
      721 ggctggcgca ttacgcgaca aaaacccgcg ccaggcgtcc catggacgac cagtaaaaat
      781 cgcggcccca accaacactc ggaaacacca cgccctgggt aatgcccccc gtgcggacac
      841 gccctagttc cgcccccgtc tcgatatcca gcacgaccac ctcttcgccg cgctcgcggt
      901 agtcattgac tgcaatttga cccgtttggc ggaacaaaac catgtggctt gcacaaccaa
      961 atttcttctt ggtccacaac agcgtgaagg cacgcaacgc ggtatcgaac cgccaggccg
     1021 ccaggtaggc attggcggag tcgtaggcca cgacaatact gcggtcgatg tcaaacaggg
     1081 gcggattggt cacactgccg gcgggcaagc cgctgactgt cctcaccgac gtatcggtgg
     1141 aatcctgcat cgatacccga atcagccgat tggaggtttt actcaccccg gcaccgagca
     1201 tggtgtaaag atagcggtgc ttgccgttgt ccatgaacca ggcgttctgg ccatcgagca
     1261 ccacatccca tccaaacgtc tgccgggtct ggtgggtata gtcgaaaaac cagtcgcagt
     1321 cccgttccag ccgcatcgtc aggtcgttcc agtgaaagcg aaaaatggtt cgcacaccca
     1381 ccacgtacac ggtgttgccc acggcgctca gtcgggctac cgacggttct ggacattcgg
     1441 tttcgctgca gaccggtgca agggaatccg ggtcaagcac cgtcaggtac gcgttttcgg
     1501 tctccgacag gttcttggtc acgaccagac cgttgtccag caccacgaac gagttgtagg
     1561 gggcattgac cggcagctcg cgtgacttca acaggctgca attgcggctc agggtgtggg
     1621 cgtaacgccc gtacaccacc accagttgcc cattgcggtg taccgccatt ccgccgggcc
     1681 acatggggcc accgggcaac cggggtgagc gctccagtgt ctggagggtc agcggatcta
     1741 tgcgttccac gcacgaagtg gtgggcaaac ccaggcgggc ccgcaaggcc gagtgggtca
     1801 gcagaaacac ctctcccggt gcccccagca ccgtcatggt ggacagcagc gtgttgcgcg
     1861 tgacacaacc caatttatca cctggctcca gcgcaatctc acctccaccg ctggccatct
     1921 gcaatcgctg cggcccaccg tcctcggcag gccagggact gtcgaaatat ccgcttgcgc
     1981 tcatggctgc acgcgatgcc tgcgacggcg gtagacaacc agccccacca gcacaaccag
     2041 cgccacggcc gcaggaatcc gcgcccgggg cagatacaca ttcaccaggg aattgatcag
     2101 cacctgattg cgcggctcgt agccttcggg catgggcagc accccattgg cctgggcgta
     2161 ggcgtcgtag tccgcacgca tgcgttgaaa cgcgtcgggt tggctgctct gcaggtcctg
     2221 tgtttcgccg gggtccttga cgatgtcgta gaggtgccac tggccatcgc ccactggagc
     2281 catattgcgt gtgagcttca ggtcgccacg gtacacggcc atattgcccg agagttcata
     2341 ccccacgggc tcatccaccg gatgcgcccg cacatcggtc gacttcagca caggcatcag
     2401 gctgcgaccc gtcatggcct ccaccgactg tcccttgtac tgcgttccag gatgctgcac
     2461 gcccgccaga tccagcaggg tcggcgcaat gtccttgacg tgggcaaact tcgggtacac
     2521 cgcatcggac cgcatgccgg gcacgcccga gacaatcagg ggcgtgcgca cccctccttc
     2581 gcccgaatag aacttgtagg tcgacagcgg cgatgcagac gcactggccc agctcggccc
     2641 aatggttccg taggccccct tgtcaccgag cttttcaaga tcgttggtgt attcagtcgc
     2701 cagccacagt ctggctgacg taatcgcata gggatcggaa gcttccgaac cgttgtcgga
     2761 gaggaatacg aacaccgtgt tgtcatactg cccgctctct ttgagatacg ccaccagccg
     2821 tcctacatgg tggtccatgg catcggccat gccggcatac acctccatgc ggcgcacttc
     2881 gtactgcttt tgctcaggcg acagcgcatc ccagtcggtc gtcgtgggca tggtcaccat
     2941 cggagcatcc ttgggcacca ggcccagcgc agcagcccgg tcccgccggg ctttgcgcag
     3001 cgcagtccag ccgtccttgt agacgccgcg gtacttgtcg atgaattcgc gtggcgcctg
     3061 caccggaata tggttggcct ggaatgccag ataggcatag aagggtttgg ccgacgtcgc
     3121 atcctgacgc agatagtcga tggttcggga cacgtaatac tcggaagagt agtagtcctt
     3181 gggcatggcc acttccttgc cattctcaaa ccagtacacc ttgtccgtca gcgccatgta
     3241 gcgtttgtca gtctcccagt tgtccgagcc gctgtcgccc tgcaccagcg agcggtcaaa
     3301 gccgcgggcg ttgggcaggt tgtacggctc cttgccgacg tgccacttgc ccactgcata
     3361 ggtacggtag ccgccatcct gcaacaggga actcaccgtg accacattct ggttcagcac
     3421 cgtgaggtag ccgggttttc cgtaatgcga ccggggcaca ctctcgcgca tggctcccac
     3481 accggtgcgg tggctgtcca cgccggtcat cagcatggca cgcgtgggtg aacactctgc
     3541 ggctacgtgg aaattcgaga accgcatgcc cttacgcgcc agggcatcca ggttgggggt
     3601 ggcaatctcg ctgccgaatg accccacatc actaaagccc cagtcgtcgg cgaccatcac
     3661 cacaatattg ggacgtttgg gttctgcggc tccggcaggc aatgcactcc caacaacgga
     3721 tagcagtaac cacagggcaa agggaattct ggacatgctg gtttctcgct gaagcttcta
     3781 ccgtcaggcc gatcgcgcct g
//
LOCUS       DAOSJM010000235         3798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21174_length_3798_cov_3.726423, whole genome shotgun sequence.
ACCESSION   DAOSJM010000235 DAOSJM010000000
VERSION     DAOSJM010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21174_length_3798_cov_3.726423"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKX82_08830"
     CDS             <1..218
                     /locus_tag="PKX82_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HOF52397.1"
                     /translation="NSGWPMAAMALNLGVALRKPGVYVLNPQGKPASAAHTALALRFA
                     SKALLALVVFAQTAMILVAFLSRGISP"
     gene            215..1258
                     /locus_tag="PKX82_08835"
     CDS             215..1258
                     /locus_tag="PKX82_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF52398.1"
                     /translation="MSAPLRTHGGPDAMGPARYDFSTNSNACGPCPQALQAVQQADAT
                     RYPDASYTELRATLAQFHGVEPGRIVLAGSASEFIFRITAWVRQHGGQTVHLPVHAYG
                     DYAHAAQAWGLTTMSDADAADLVWACEPSSPLGLAHRGWPAWLVSQGVPGAGGDAQLV
                     LDCAYAPLRLQGAPSLSAQQRDRVWQLFSPNKALGLTGVRASYAIAPLAAQAAAEKLD
                     ALAPSWVIGAHGVAMLQAWTRSDTQAWLHTSLRTLQDWKVRQEQVLQSLGWTVLTSDA
                     TFFCAQLPAGVDVRALCTQLRAQGIQLRDATSFGLPGWVRLGTLPPQAQDALVEALEM
                     AHSRAMPLMESSQ"
     gene            1255..2856
                     /locus_tag="PKX82_08840"
     CDS             1255..2856
                     /locus_tag="PKX82_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOF52399.1"
                     /translation="MTAKCIMVLGTTSGAGKSWLTTALCRYYARQGLKVAPFKAQNMS
                     NNARVVASENGQGEIGSAQYFQALAARAIPDVRMNPLLLKPERDTHSQVVLMGQVDAE
                     LTAMPWRGRSLKVWPQIAAALDALRAENDVVVIEGAGSPAEINLSSSDIVNMRVASHC
                     HAACLLVTDIDRGGAFAHLYGTWALLPEDERRLIKGFVLNKFRGDAALLAPAPQMLQD
                     LTGIPTVATLPMWWQHGLPEEDGVFDDRSVTKGAVNMTVAVIAYPRISNLDEFQPLKN
                     IPGLKLQWVRSPSELAGLQPSDWIILPGSKHTSGDLEWLRSQGLDAAVARHAQQGGAV
                     LGVCGGLQMLGEALIDTHGIDGNAPGLGLLPLVTVFAEDKTVRHTQTTFSQGFHSSAQ
                     GGEAGGLILPNAQKSPPGFASPRLDGDTGADSVPVSPWAALAGVSVAGYEIHHGQTQQ
                     HVAMAAAGNVAHAVLPDGLGWCSAQGNVMGLYLHGLFEDPNALQALFGAQLQGAVPTL
                     DTVFEGLADYIGAHFAPGVLQDLLV"
     gene            2884..>3798
                     /gene="cobT"
                     /locus_tag="PKX82_08845"
     CDS             2884..>3798
                     /gene="cobT"
                     /locus_tag="PKX82_08845"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOF52400.1"
                     /translation="MTTFLPTLADISDAALTQALQHTLDNKTKPQGSLGRLEDLGLKI
                     GQILGTTAPVLEQPQMVVFAGDHGLTARGISAFPNDVTWQMVENFLAGGAAVAVLARQ
                     NGLALTVVDCGVKHDFLAGLPAGAERPGLQVRKAKGAEHGTADASAQPAMTDAQRDEA
                     LHHGMELVKGLPGNALLLGEMGIGNTSAASLLLSRLAGLDIAVCTGAGTGLDAPAVQR
                     KTAVLREVLERHVDATAPLDALAALGGFEIATMVGAVLQAAVERRVIVVDGFIATSAV
                     VVAHALQPLVLQRCVFAHRSGERGHEFML"
BASE COUNT          610 a         1149 c         1332 g          707 t
ORIGIN      
        1 ccaacagcgg ctggcccatg gcggccatgg ccctgaacct gggcgtggcc ttgcgcaaac
       61 ccggtgttta tgtgctcaac ccgcagggca agcctgccag cgctgcgcac accgcgttgg
      121 ccctgcgttt tgcatcaaaa gcactgctag ccctcgtggt atttgcgcag acagctatga
      181 ttttggtagc atttttgagt aggggaatct caccatgagc gcgccactgc gcacccacgg
      241 cgggccggac gcaatggggc ctgcgcgtta cgacttctcc accaacagca atgcctgtgg
      301 accctgcccg caggcgctgc aggcggtgca gcaggcggac gccacgcgtt atcccgacgc
      361 cagttacacg gaattgcgcg ccacgttggc gcagttccat ggtgttgaac ctgggcgcat
      421 cgtgctggcg ggcagcgcca gcgagttcat ctttcgcatc acggcgtggg tgcggcagca
      481 cggtggccag acggtgcacc tgccggtcca tgcctacggt gactacgccc acgctgcgca
      541 ggcctggggc ctcacaacaa tgtctgatgc agacgctgcc gatctggtgt gggcctgcga
      601 gccttccagt cctttgggcc tggcgcaccg gggctggccg gcgtggctgg tttcgcaagg
      661 tgtgcctggc gccggtggtg atgctcagct ggtgctggac tgcgcttatg cgcccctgcg
      721 tttgcagggt gcgcccagcc tgtcggcgca gcagcgtgac cgggtgtggc agctgttttc
      781 gcccaacaag gcgctggggc tcaccggggt gcgtgcgtcc tacgccattg cgccgctggc
      841 agcgcaggcc gccgcggaga aactggatgc gctggctccg tcctgggtca tcggcgcgca
      901 tggtgtggcc atgttgcagg cctggacacg gagcgacacg caggcctggc tgcacaccag
      961 cctgcggacc ctgcaggact ggaaagtgcg gcaggagcag gtgctgcaat cgctgggctg
     1021 gaccgtgctc actagtgacg ccacgttctt ctgcgcccaa ctgcccgcgg gtgtcgacgt
     1081 tcgcgcgctg tgtacccagt tgcgtgcgca aggcattcag ctgcgcgatg ctacgtcgtt
     1141 cggcctgcca ggctgggtgc gtctggggac cctgccaccg caggcgcagg acgccctggt
     1201 cgaagcccta gagatggccc attcacgcgc catgccactg atggagtcga gccaatgacc
     1261 gccaaatgca tcatggtgct gggcaccacc agcggagctg gcaaaagttg gctgaccacc
     1321 gccttgtgcc gctactacgc acgtcagggg ctgaaggttg cgccctttaa agcgcagaac
     1381 atgagcaaca acgccagggt ggtggcctca gaaaatggcc agggagaaat cggaagtgcg
     1441 cagtatttcc aggcgctggc ggccagggcc attcctgatg tgcgcatgaa tccgctgctc
     1501 ctgaagcccg aacgcgatac ccatagccag gtggtgctga tggggcaggt ggatgctgag
     1561 ctcaccgcca tgccctggcg cggacgcagc ctcaaggtct ggccgcagat tgccgccgcg
     1621 ctggacgcgt tgcgcgccga gaacgatgtg gtggtgattg agggcgcggg gtcgcccgcc
     1681 gagatcaacc tgtccagcag cgacatcgtg aacatgcgcg tggccagcca ctgccatgcc
     1741 gcttgtttgc tggtgaccga tattgaccgg ggcggggcgt tcgcccacct gtacggcacc
     1801 tgggcgctgt tgcccgagga cgaacgcagg ctgatcaagg gctttgtgct caacaagttc
     1861 cgcggggatg cagccctgct ggcgccagcg ccgcagatgc tgcaggacct gaccgggatt
     1921 cccaccgtgg ccacgctgcc gatgtggtgg cagcacgggt tgcccgaaga agacggtgtg
     1981 ttcgatgacc gcagtgtgac caaaggtgca gtcaacatga cggtggccgt cattgcctac
     2041 ccgcgcatca gcaacctcga cgagttccag ccgctcaaga acattccggg cctcaagctg
     2101 cagtgggtgc gcagcccgtc cgagctggcg gggctccagc cgtcggactg gatcatcctg
     2161 cccggctcca agcacaccag tggcgatctg gagtggctgc gttcgcaggg gctggatgcg
     2221 gccgtggccc ggcatgccca gcagggtggg gcggtgttgg gcgtgtgtgg cgggctgcag
     2281 atgctgggcg aggcgctgat cgacacgcac gggattgacg gcaatgcgcc gggactgggg
     2341 ctgttgccgc ttgtcaccgt gtttgcagaa gacaagacgg tacggcatac gcagaccact
     2401 ttttcgcagg gtttccattc gtctgcgcag ggaggcgaag ccggggggct catactgcca
     2461 aacgcgcaga aatcgccccc cggcttcgcc tccccgcgct tggatggtga cactggtgcg
     2521 gattcagtcc ccgtttcgcc ttgggctgcg ctggcgggtg tatccgtcgc cggctatgaa
     2581 atccaccacg gccagacgca gcagcatgtg gccatggcag cggcgggtaa cgtggcgcac
     2641 gcggtgctgc ctgacgggct gggttggtgc agtgcgcagg gcaacgtgat gggcttgtac
     2701 ctgcatgggc tgttcgagga ccccaacgca ctgcaggcgc tgtttggtgc gcagttgcag
     2761 ggggctgtgc ccacgctcga tacggttttt gaaggtctgg cggactacat cggtgcgcac
     2821 ttcgcgcccg gtgtgctgca ggacctgctg gtttgacttc ctttttttga aagcgatatt
     2881 tccatgacca cctttttgcc aacccttgcc gatatttccg atgccgcact gacgcaggcg
     2941 ttgcagcaca cgctggacaa caagaccaag ccgcagggct cgctcggtcg tctggaagac
     3001 ctggggctga agatcggtca gattctgggc accacggcgc cggtgctgga gcagccgcag
     3061 atggtggtgt ttgccgggga ccatggcctg acggcgcgcg gtatttcggc gtttccgaac
     3121 gacgtgacct ggcagatggt ggagaacttt ctggccggtg gcgccgcggt ggcggtgctg
     3181 gcgcggcaaa acgggctggc gctcacggtg gtggactgtg gtgtgaagca cgactttctg
     3241 gcgggtctgc ctgccggtgc cgagcggccg ggcctgcagg tacgcaaggc caagggtgcc
     3301 gagcacggca ccgcggatgc gtcggcacaa ccggccatga cggacgccca gcgtgacgaa
     3361 gccctgcacc acggcatgga actggtcaaa ggcctgccgg gcaatgcgct gctgttgggt
     3421 gagatgggca ttggcaacac ctcggcagcg tcgctgctgt tgtcgcgtct ggctgggctg
     3481 gacattgcgg tgtgcaccgg tgcaggcacc ggtctcgatg cgcctgcggt gcagcgcaag
     3541 accgccgtgc tgcgcgaagt gctggagcgc cacgtggatg cgactgcacc actggacgca
     3601 ctggccgcgc tgggaggatt cgagattgcc accatggtgg gtgcggtgct gcaggcagcg
     3661 gttgagcgcc gtgtgatcgt ggtggacggg ttcatcgcca ccagcgccgt agtggtggcc
     3721 catgccctgc agccgctggt gctgcagcgt tgcgtgtttg cccaccgttc tggcgagcgg
     3781 ggccatgagt tcatgttg
//
LOCUS       DAOSJM010000236         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21186_length_3795_cov_4.247861, whole genome shotgun sequence.
ACCESSION   DAOSJM010000236 DAOSJM010000000
VERSION     DAOSJM010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21186_length_3795_cov_4.247861"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="PKX82_08850"
     CDS             complement(<1..1451)
                     /locus_tag="PKX82_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOF52401.1"
                     /translation="MNVFKLLSLCMAVYLAGCATAPPPQTATSPDGDDALTLSASREL
                     PRTAAPQPRRSVAPPPSTTASDRVPLTPITAHQTASRSVVALAPPVDLWDRIRRGYAM
                     PDLDTDLVRDREQWYATRPDYIFRMTERSKKYLFHIVEELELRGMPTELALLPFIESA
                     FNPQAVSGAKAAGMWQFMPATGKYFELKQNAFRDDRRDVLASTRAALDYLQKLYGMFG
                     DWHLALAAYNWGEGSVGRAIAKNQRAGTGTAYTDLNMPMETRFYVPKLQAVKNIVTNP
                     AAFNSKLPLIENHPYFQTVTIRRDIDVALVACLAEVPLDDFKALNPSVNRPVILAAGT
                     PQILLPWDNAEVFQGNLESYSGGRLASWTVWVAPSTLRPADAARRVGMTESDLRAVNN
                     IPPRVVIRAGSTLLVPRSVHVAHDVTEKVADNGQVTLAPEVVLKRSTVRAGKGESVAT
                     IARKYRVSTDTVAQWNKVSASATFKAGQQVVVFL"
     gene            complement(1448..2218)
                     /gene="gloB"
                     /locus_tag="PKX82_08855"
     CDS             complement(1448..2218)
                     /gene="gloB"
                     /locus_tag="PKX82_08855"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOF52402.1"
                     /translation="MKLIPLPAFDDNYIWMLHDGQQALVVDPGDAQPVFDALQHEGVQ
                     LQAILVTHHHGDHTGGVAALRASTGATVYGPAREDIPGPLTALADGQAVTALGLQFEV
                     IDVPGHTAGHIAYVCAAMDGQPLLFCGDTLFSAGCGRLFEGTPAQMLASLNRLAALPP
                     DTRVCCTHEYTLSNLKFALEVDPDNADLIAYSAHCHALRAQGQPTLPSSIALERKINP
                     FLRSSEPALIAAARRFDAASVEQNGAFATLRQWKNQYR"
     gene            2217..3011
                     /locus_tag="PKX82_08860"
     CDS             2217..3011
                     /locus_tag="PKX82_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF52403.1"
                     /translation="MSDQIIDLHDFFQTPAGQYLLEWERAHVDAAVADIFGYHALQLG
                     VPELDGLRANRMPHQWLATDHITADLNTTGPHTAFMTDFSALPFSASSIDLVLLPHSL
                     EFSADPHATLREVERVLVPEGRVVICGLNPYSLWGLRQQRAHIYNRFAGGGKLFLPDC
                     GEFIGYWRLRDWLQLLGFEVEVGTFGCYRPAVDHERWLQRYEWMDRAGPRWWPILGAA
                     YFLVAVKRVRGMRLLGAAWKAAPVRGAKPVSVINRATDFQIEESKE"
     gene            3008..3460
                     /gene="rnhA"
                     /locus_tag="PKX82_08865"
     CDS             3008..3460
                     /gene="rnhA"
                     /locus_tag="PKX82_08865"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOF52404.1"
                     /translation="MNAVVIYTDGACKGNPGPGGWGALLRSSDGTEKELFGGELETTN
                     NRMEMTAVIQALAILKRPCAVTLHVDSQYVLKGMTEWLAGWKAKGWKTAAKQPVKNVD
                     LWQALDKLVSQAGHTIDWRWVKGHAGDPGNERADALANRGVELALRQR"
     gene            3477..>3795
                     /locus_tag="PKX82_08870"
     CDS             3477..>3795
                     /locus_tag="PKX82_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HOF52405.1"
                     /translation="MASWAFYPAVAAIGIAVAGGAAWWYQSQVKGPNEVVSSNGATSG
                     AAAPAPKTGASGAGAPRVAGVEVAKVEKMTLQDDAQSVGTLRSRQSVMLRPEVAGRIS
                     QIGF"
BASE COUNT          672 a         1067 c         1306 g          750 t
ORIGIN      
        1 aggaagacca ccacctgttg ccccgccttg aacgtggcgc tggcactgac cttgttccac
       61 tgtgcgacgg tgtccgtgct gacccggtat ttgcgcgcaa tggtcgccac gctctcaccc
      121 ttgcccgcgc gcacggtgct gcgcttcaga accacttcgg gggccagggt gacctgtccg
      181 ttgtcggcca ctttctcggt gacatcgtgt gccacatgga ctgagcgggg aaccaatagg
      241 gtggagcctg cccggatgac cacccgtggc ggaatattgt tgacagcacg cagatccgat
      301 tccgtcatgc ccacgcgcct ggcagcatcg gcagggcgca gggtactggg ggccacccag
      361 accgtccagc ttgccaggcg tccaccgctg tagctttcca gattgccctg gaagacttcc
      421 gcgttatccc acggcaggag aatctggggg gtgcccgcag ccaggatcac cggacggttg
      481 acggaagggt tgagtgcctt gaagtcgtcc aggggcactt cggccaggca cgcgaccagt
      541 gccacatcga tgtcgcgccg gatcgtgact gtctggaaat aggggtggtt ctcaatcagg
      601 ggcagcttgg agttgaaggc cgccgggttg gtgacgatgt ttttgacggc ctgcagtttg
      661 ggcacataga aacgtgtttc catgggcatg ttgaggtcgg tgtaggccgt acccgtgcct
      721 gcgcgctggt tcttggcgat cgcgcgcccc acactgcctt cgccccagtt gtaggcggcc
      781 agggccagat gccagtcgcc aaacatgcca tagagttttt gcagatagtc cagtgccgcg
      841 cgggtggaag ccagcacgtc gcgccggtcg tcacgaaagg cgttttgctt gagttcaaag
      901 tacttgccgg tggcaggcat gaattgccac atgcccgcgg ccttggcgcc cgacacggcc
      961 tggggattga acgcgctctc gatgaatggc agcaaggcca gttcggtcgg catgccgcgc
     1021 aactccagtt cttcaacaat gtggaacagg tacttcttgg agcgctcggt catgcggaag
     1081 atgtagtcgg gccgcgtggc gtaccactgc tcgcggtcac gcaccaggtc ggtatccaga
     1141 tcgggcatgg cgtagccgcg gcggatgcgg tcccacaggt cgactggcgg cgccagcgcg
     1201 acgacgctgc gcgatgcggt ctggtgcgct gtgatgggcg tcagcggcac cctgtcggac
     1261 gcggtggtcg atggcggcgg ggccacgctg cgacggggtt gcggcgcagc cgtgcggggc
     1321 agttcgcggc tggcagacag ggtgagggca tcatccccat cgggcgacgt tgccgtctgg
     1381 gggggcggcg cagtcgcaca gcctgccagg tacaccgcca tgcacaggct gagtagtttg
     1441 aaaacattca tcggtattgg ttcttccatt ggcgcaatgt ggcaaatgcg ccgttctgtt
     1501 ccacgctggc cgcatcaaag cggcgcgctg ccgcaatcag ggcgggttcg ctggagcgca
     1561 gaaaaggatt gatttttctt tccagcgcaa tgctgctggg cagggtgggt tggccttggg
     1621 cgcgcagggc atggcagtgg gcgctgtagg cgatcaggtc ggcattgtcc gggtcgacct
     1681 caagcgcaaa cttgaggttg ctcagggtgt actcgtgggt gcagcacacc cgcgtgtccg
     1741 gggggagcgc cgcaaggcgg ttgagtgacg ccagcatctg tgccggcgtg ccctcaaaga
     1801 ggcgtccgca gccggcgctg aacagggtgt caccacaaaa cagcagcggt tgcccgtcca
     1861 tggcggcaca gacgtaggca atgtgccccg cagtgtgccc cggaacatcg atcacctcga
     1921 actgcaggcc cagtgccgtg acggcttgcc cgtcagccag ggctgtcaag ggtccgggaa
     1981 tgtcttctct ggccgggcca tagaccgtgg cacctgtgga tgcacgcagt gctgccacgc
     2041 cgccggtgtg atcgccgtgg tggtgggtga ctagaatggc ctgcagttgc acgccttcgt
     2101 gctggagtgc gtcgaacaca ggttgcgcat caccggggtc caccaccagc gcttgctgac
     2161 cgtcgtgcaa catccagatg tagttgtcgt caaaagcggg tagcggtatt aacttcatga
     2221 gcgaccagat tatagatttg catgattttt ttcagacccc tgcagggcag tacctgctgg
     2281 agtgggagcg tgcgcatgtg gatgccgccg tggccgacat atttggctac cacgcgctgc
     2341 agctgggggt tcccgagctc gacggtttgc gtgccaaccg catgccccac caatggttgg
     2401 ctacggacca catcacggcc gatttgaaca cgaccggccc gcataccgcc ttcatgacgg
     2461 atttttctgc cttgccgttt tccgccagca gcattgacct ggtgcttttg ccccattcgc
     2521 tggagttcag tgccgacccg catgcgacgc tgcgcgaagt ggaacgggtg ctggtgcccg
     2581 aggggcgggt ggtgatttgc ggcctcaacc cctacagttt gtggggcctt cggcagcagc
     2641 gtgcgcacat ctacaaccgt tttgcgggtg ggggcaagct gtttctgccc gattgtggtg
     2701 agttcatcgg gtactggcgt ttgcgggact ggctgcaact tctgggtttt gaggttgagg
     2761 tgggaacctt cggctgctat cgcccggctg tggaccatga gcggtggctc caacgctacg
     2821 agtggatgga ccgtgcgggg ccgcgctggt ggcccattct gggcgccgcg tattttttgg
     2881 tggcagtgaa gcgcgtgcgg ggcatgcgcc tgctgggggc ggcctggaaa gcggcgcctg
     2941 tgcggggagc caaaccggta tcggtgatca accgggctac ggattttcag atagaggaga
     3001 gtaaagagtg aatgcggttg tgatttacac cgacggggct tgcaagggaa accccggacc
     3061 aggtggttgg ggggcgcttt tgcgttcgtc cgacggtacc gaaaaggagc tgtttggtgg
     3121 tgagctggaa acgaccaaca accgcatgga aatgaccgcg gtgatccagg ccttggccat
     3181 actgaagcgc ccctgcgccg tcacgctgca tgtggacagc cagtatgtgc tgaagggcat
     3241 gacagagtgg ctggccggct ggaaggccaa gggctggaag accgctgcca aacagccggt
     3301 gaaaaacgtc gatttgtggc aggcgttgga caaactggtg tcacaggccg gccatacaat
     3361 cgattggcgt tgggtcaaag gccacgcagg ggatcccggc aacgagcgtg ccgacgcgct
     3421 ggccaaccgc ggtgtggagt tggcgctgcg acaaagataa caaggacgat acatcgatgg
     3481 catcctgggc tttttatcct gccgttgcgg cgatcggcat tgccgttgca ggtggtgcag
     3541 cgtggtggta ccagtcgcag gtcaagggac ccaacgaagt ggtttccagc aacggtgcaa
     3601 cgtctggcgc tgcagcgcct gctccaaaaa caggagcgtc tggagcaggt gcaccgcggg
     3661 ttgcgggcgt agaagtcgcc aaggttgaaa aaatgacgct gcaggacgat gcccagtcag
     3721 tgggcaccct gcgctcgcgc cagagcgtga tgctccggcc ggaagtggct ggccgcatca
     3781 gccagatcgg cttta
//
LOCUS       DAOSJM010000237         3789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21229_length_3789_cov_3.902517, whole genome shotgun sequence.
ACCESSION   DAOSJM010000237 DAOSJM010000000
VERSION     DAOSJM010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21229_length_3789_cov_3.902517"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PKX82_08875"
     CDS             <1..856
                     /locus_tag="PKX82_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOF52406.1"
                     /translation="VRLLSMCTHGMLKLLRIDTTDNRAVTEAEISASLEEGVDAGIIE
                     EHEHQMVRNVFHLDDRTLASMMLPRDDIAWLDASATVTECLAQAKASAATGLHSWYPV
                     CKGSLDDVVGIVSVAVLLQRGERYPYGVGALVEPAVFVPETLTGMELIEQFRIKSARM
                     VFVVDEYGVVQGLMTPHDVLEAITGELKPDAQTQAWAVEEADGSWSLDGLMPIGELKA
                     RLDIDEDLPQEDRGRYNTLAGLLMVVTGDLPSQGDQIECAGWQFEVTTLEGRRVDKVR
                     ARRLPPAE"
     gene            complement(858..1628)
                     /locus_tag="PKX82_08880"
     CDS             complement(858..1628)
                     /locus_tag="PKX82_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOF52407.1"
                     /translation="MFVLSKILALLTQPIGWVVVLLALALFWFKRRPRRARGLLALSI
                     VLLLLLGWVPLPDMLLRHLEGQYAEVAPNADLRGYTGVVVLGGGTESGLVSQAHVHPL
                     VNDAAERMTAAVALLQRHPHLKLVYTGGEGALLGTGPSEAARAQGFFESLGITGPRMR
                     YEAVSRNTYENAVLTARLEGVDITQRWLLVTSAWHMPRSMGTFVKAGWNVTAYPVDFR
                     TAPVLPWTDYALTAGIHHWQIALHEYLGLLSYRITGRL"
     gene            1781..2359
                     /locus_tag="PKX82_08885"
     CDS             1781..2359
                     /locus_tag="PKX82_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52408.1"
                     /translation="MQFLRNPLAIALAALSLSMGGTAVAQTTVTAQATVGTTTGSTAG
                     TTTAPTTQQVPRNFSTDLAKASLAKQGITNPTKAQLDAERKSINAQRADGKGWGVIAQ
                     SLGLNLGQVVSDANRARRDARKAESDRADKGEKGKKSREHGKAADQTTGTTKYGAKPS
                     GSSTSGKSGGDHGGGGHGGGNGGGGGGGKGGK"
     gene            complement(2422..2802)
                     /locus_tag="PKX82_08890"
     CDS             complement(2422..2802)
                     /locus_tag="PKX82_08890"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOF52409.1"
                     /translation="MKPFVRKLLSRRLWRTLLVLLWMVVSYLALTPSPKQGPDLGWDK
                     LNHTFAFVALAFAAWLGFAQAQRSQRLWMLVLLAYGGGIEILQLYVPGRSCEWGDLLA
                     DAVGITLGASLGIFITKSASSPRQ"
     gene            2907..>3789
                     /locus_tag="PKX82_08895"
     CDS             2907..>3789
                     /locus_tag="PKX82_08895"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HOF52410.1"
                     /translation="MKRFFLVLALCAGFAWADDLEDGRAAYQAKNYVTAKAKFLAAAK
                     TGDVRAQNGLGWLHDKGLGVAQDTREAMKWYRLAADQGNVFAQSRLAVAYARGDGVPQ
                     NNTESVRWNRMAAEQGFADAQANLGWAYDQGLGIPQDFAEAVKWYRLAAEQGHPIAQS
                     NLGQMYGNGQGVQQDYKEAVKWYTLAAAQGFADAQANLGWVHAQGYSVPVDYREAAKW
                     YRLAADQGHAAAQANLGWLYDKGLGVSQDYQEAVQWYRRAADLGYALAQYGLGVAYQY
                     GRGVQANPVVAYALYSLS"
BASE COUNT          768 a         1172 c         1238 g          611 t
ORIGIN      
        1 tgtgcgcctg ctctcgatgt gtacgcatgg catgctcaag ctgctgcgca ttgacaccac
       61 cgacaaccgc gcagtgaccg aggccgagat ttctgccagc ctcgaagaag gcgtggacgc
      121 cggcatcatc gaagagcacg aacaccagat ggtgcgcaac gtgttccatc tggatgaccg
      181 cacgctggca tccatgatgc tgccgcgtga cgacatcgcc tggctcgatg cgtctgccac
      241 cgtgaccgaa tgcctggccc aggccaaagc cagcgctgcc accggtctgc attcctggta
      301 cccggtgtgc aagggttcgc tggacgatgt ggtgggcatt gtcagcgtgg ccgtgctgct
      361 gcagcgtggc gaacgctatc cctatggcgt tggcgcgctg gtcgaacccg ccgtctttgt
      421 gcccgaaaca ctgactggca tggagctgat cgaacagttc cgcatcaagt cggcccggat
      481 ggtgtttgtg gtggacgaat acggggtggt gcagggtctg atgaccccgc acgatgtgct
      541 ggaagccatt accggcgaac tcaagccgga cgcccagacg caggcctggg ctgtggaaga
      601 ggcagatgga tcctggtcac tcgacgggct gatgccgatt ggcgagctca aggcgcgcct
      661 ggacattgat gaagacctgc cacaagaaga ccgtgggcgt tacaacacat tggctggcct
      721 gctgatggtg gttacgggtg atttgccatc gcagggggac cagatcgaat gcgccggctg
      781 gcaattcgaa gtcaccacac tcgaaggccg gcgtgtcgac aaggtgcggg cgcggcgcct
      841 gccgccagcg gagtgaccta cagccgtccg gtgatccggt aggacagcag ccccaggtat
      901 tcgtgcagtg caatctgcca gtggtggatg cctgcagtca gagcgtaatc ggtccagggt
      961 aacaccggtg cagtgcggaa atccaccggg tacgccgtca cgttccagcc cgctttcaca
     1021 aacgtgccca tggagcgcgg catgtgccac gccgatgtca ccagcagcca gcgctgggtg
     1081 atgtcgactc cttcgagtcg ggcggtcagt acggcgttct cgtaggtgtt gcgggagacg
     1141 gcctcgtagc gcatccgggg tcccgtgata cccaggctct cgaaaaagcc ctgcgcgcgc
     1201 gccgcttcac tcgggccggt gccaagcagg gcgccttcgc cgccggtgta caccagcttc
     1261 aggtgcggat ggcgctgcaa gagcgccaca gcggccgtca tgcgctcggc cgcatcgttg
     1321 accaatgggt gcacatgcgc ctgcgacacc agcccggact cggtgccgcc gcccagcacg
     1381 accacgccgg tgtagccgcg caggtcggcg ttaggggcca cttccgcgta ctggccttcc
     1441 aggtggcgca gcagcatgtc gggcagtggc acccagccca gcaacagcaa tagcacgatg
     1501 gacaacgcca gcaggccacg cgcccgccgg ggcctgcgtt tgaaccagaa cagcgccagc
     1561 gccagcaaca ccaccaccca cccaatgggc tgcgtcaaca gggcaagaat cttggaaaga
     1621 acaaacattg ggggtagcgg cggcaaaggc aggcatgcta ccacccgccc cctgtttgca
     1681 tacccccagg ggaaacatcg cgttacatct tcgtggggct ggttggcggt ggccagacct
     1741 agaatgaaag cgtcatcttt gatttttctg gagcacctct atgcaatttc tgcgaaaccc
     1801 cctggccatt gcgctggctg ccctgtcgtt gagcatgggt ggcactgccg tggcacagac
     1861 cacggtcact gcccaagcca ctgtgggcac caccaccggt tccactgctg gaacaaccac
     1921 ggcgccgacc acacagcagg ttcccagaaa cttctccacc gatctggcca aggccagcct
     1981 ggccaagcag ggcatcacca accccaccaa agcgcaactc gatgccgagc gcaagtccat
     2041 caatgcccag cgcgccgacg gcaagggctg gggcgtgatc gcccagtccc tgggtctgaa
     2101 cctgggccag gtggtcagtg acgccaaccg tgcgcggcgc gacgcccgca aggccgagag
     2161 cgatcgcgct gacaagggcg aaaaaggcaa aaagagccgc gagcacggca aggcggcgga
     2221 ccagaccacc ggcaccacca agtacggcgc aaaaccctcc ggtagcagca ccagcggcaa
     2281 gtcgggcggt gaccacggtg gaggcggcca cggcggtggc aatggaggtg gtgggggcgg
     2341 aggcaagggt ggcaaataag ccattgcact cctaagcatt cccgggaaaa gcctgctatc
     2401 tcattgatag caggcttttg tttattgacg ggggctagag gctgattttg ttataaatat
     2461 ccccagagac gcgcccaagg tgatacccac cgcgtccgcc agcaggtccc cccattcgca
     2521 cgaacggccg ggcacataca gctgcaggat ctcgatgcct ccgccatagg ccagcagcac
     2581 cagcatccac aggcgctgac tgcgctgcgc ctgcgcaaaa cccagccacg cggcaaacgc
     2641 cagtgccaca aaggcgaatg tgtggttgag cttgtcccac cccaggtcgg ggccttgttt
     2701 gggcgagggg gtcagcgcca gatagctcac caccatccac agcaggacca gcagagtgcg
     2761 ccagaggcgc ctggaaagaa gcttgcgaac aaatggtttc atcggaagga aggagtgcag
     2821 ttgcgccgag gttagccgaa gaccggagcg tctatgcata atcaccgcca caaaagacag
     2881 ctgctgccgc agaacaaagg gatggcatga aacgattttt tctggtgttg gccttgtgtg
     2941 ccggttttgc gtgggccgac gatctggaag acgggcgtgc tgcctaccag gccaaaaact
     3001 acgtgaccgc caaggcaaag tttcttgccg ccgccaaaac cggggatgtg cgcgcgcaga
     3061 acggtctggg ctggctgcac gacaaggggc tgggtgtggc gcaggacacc cgggaagcga
     3121 tgaagtggta ccgcctggcc gctgaccagg gcaacgtctt tgcgcaaagc cgcctcgccg
     3181 tggcctacgc ccgcggtgat ggcgttccgc agaacaacac cgagtcggtg cgctggaacc
     3241 gcatggccgc agagcagggc tttgcggatg cgcaagccaa cctgggctgg gcttacgacc
     3301 aggggctggg cattccccag gactttgcgg aggccgtgaa gtggtaccgg ctggcggccg
     3361 agcagggcca tcccattgcg caatccaacc tgggccagat gtatggcaat ggccagggtg
     3421 tgcagcaaga ctacaaggaa gcggtgaagt ggtacacgct ggccgcagcg cagggctttg
     3481 cggatgcgca ggccaacctg ggctgggtgc acgcccaagg ctacagcgtg ccggtggact
     3541 accgggaggc cgccaaatgg taccggctgg ccgccgacca gggtcatgcc gcagcgcagg
     3601 ccaacctggg ctggctgtat gacaagggcc tgggtgtttc gcaggactac caggaggccg
     3661 tgcagtggta ccgccgcgcg gcagacctgg gctatgccct ggcccagtat ggcctgggcg
     3721 tggcctacca gtacgggcgc ggcgtgcagg ccaacccggt cgtcgcctat gcgctctaca
     3781 gcctgtcca
//
LOCUS       DAOSJM010000238         3787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21244_length_3787_cov_4.351018, whole genome shotgun sequence.
ACCESSION   DAOSJM010000238 DAOSJM010000000
VERSION     DAOSJM010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21244_length_3787_cov_4.351018"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="PKX82_08900"
     CDS             <1..878
                     /locus_tag="PKX82_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOF52411.1"
                     /translation="IDPQRNAAAGPALVVSPNPFYQIYEGAAYLSGAQPYFVASDPKR
                     NFAQDWDSVPDEVWARTQLLFVCSPGNPAGAVMPLGEWKKLFDLSDRFGFVIASDECY
                     SEIYFRDEPPLGGLEAAARLGRSDFRNLLAFTSLSKRSNVPGMRSGFCAGDANLVKQF
                     LLYRTYHGSAMSTVVQAASVAAWNDEQHVIDNRAKYREKFAQVTPMLSPVLDVALPDA
                     GFYLWAGVQGSDTDFARDLYALYNVTVLPGSFLAREAHGSNPGSGRVRMALVAETAEC
                     VEAAERIVQFVQSRI"
     gene            897..1721
                     /gene="dapD"
                     /locus_tag="PKX82_08905"
     CDS             897..1721
                     /gene="dapD"
                     /locus_tag="PKX82_08905"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOF52412.1"
                     /translation="MSQPLQQIIEAAWEDRANISVASAPAEVRDAVEHVLNELDAGRQ
                     RVATREGVGQWTTHQWIKKAVLLSFRLKDNEIMQAGSLGFYDKVATKFSNLSAEEMKA
                     TGVRVVPPAVARRGSYQAKGVILMPSYCNIGSYVDEGSMVDAWATVGSCAQIGKNVHL
                     SGGVGIGGVLEPMQAGPTIIEDNCFIGARSEIVEGVIIEENSVISMGVYIGQSTKIYD
                     RATGEVFYGRVPSGSVVVSGNLPSADGKYSLYCAVIVKRVDAQTRAKTSLNDLLRD"
     gene            1750..2910
                     /locus_tag="PKX82_08910"
     CDS             1750..2910
                     /locus_tag="PKX82_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOF52413.1"
                     /translation="MATNSTPGTMERILRLMSDKKASDVYLSAHSPALIRINGQCVPI
                     NSQLLPADAPKNLLAEVLPAERIAELEAKGELNMGFPVDGVGRFRVSAMRQRGSYAAV
                     IRYITVDIPPLANLSVPMMLGDLIMEKRGLILMVGATGAGKSTTLASMLDFRNEHLAG
                     HILTIEDPIEFLFKNKKAVVNQREIGTDTQSLEIALKNALRQAPDVILIGEIRDRETM
                     SAAIAYAQSGHLCLATMHANNSYQALNRILSFYPVEVRSTMLGDLAAALKAVVSQRLL
                     RTTTGARTPAVEVLLNTKLISELVEKGDFSGIKEAMEKSMAEGSQSFEHDIARLIVDG
                     TVDRKEGLAHADSPTNLMWRLQNDFTKAAQPVAAQEEDEQASFTEITLDVKH"
     gene            2996..>3787
                     /gene="dapE"
                     /locus_tag="PKX82_08915"
     CDS             2996..>3787
                     /gene="dapE"
                     /locus_tag="PKX82_08915"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HOF52414.1"
                     /translation="MTETLYLAEQLIARASVTPLDGGCQELIAKRLTACGFVCESIVS
                     GPEDFRVTNLWAKRAVARVDIAQVAINSIANTLPVKTLVFAGHTDVVPTGPLDQWTSP
                     PFTPSHRDGKLYGRGAADMKTSLAAFVVAVEEFIAAHPQPQLNIGFLLTSDEEGPAND
                     GTVVVCNTLKARGEVLDYCIVGEPTSVERTGDMIKNGRRGTMSGKLTVKGVQGHIAYP
                     HMARNPIHLVAPALAELVGVQWDDGNAFFPPTTWQVSNIHGGTGAS"
BASE COUNT          767 a         1179 c         1152 g          689 t
ORIGIN      
        1 tgattgaccc ccaacgcaat gccgccgcgg gccctgcact ggtggtcagt cccaacccgt
       61 tctaccaaat ctacgaaggt gcggcctacc tctctggcgc gcagccgtac tttgtggcgt
      121 ccgacccgaa gcgcaacttc gcccaggact gggacagcgt gcctgacgaa gtctgggccc
      181 gcacccagct gctgtttgtg tgctcgcctg gcaatcccgc cggcgcagtc atgcccctgg
      241 gcgagtggaa gaagctgttt gatctcagcg accgctttgg tttcgtgatt gcatcggacg
      301 agtgctatag cgagatctat ttccgcgacg agccgccatt gggcggtctg gaagccgctg
      361 cccgcctggg ccgcagcgat ttcaggaacc tgctggcatt taccagcctg tccaagcgca
      421 gcaatgtgcc gggcatgcgc agcggctttt gcgcgggcga tgccaatctg gtcaagcagt
      481 tcctgctgta ccgcacctac catggcagcg ccatgagcac cgtggtgcag gccgccagcg
      541 tggccgcctg gaacgacgag cagcacgtca tcgacaaccg ggccaagtac cgtgaaaaat
      601 tcgcccaggt gacgcccatg ctgtcaccag ttctggacgt ggccctgcct gatgcgggct
      661 tctatttgtg ggccggcgtc cagggcagcg acaccgactt cgcccgtgac ctgtacgctc
      721 tttacaatgt caccgtactg cccggcagtt ttcttgcgcg cgaagcgcat ggaagcaacc
      781 ccggctccgg gcgcgtgcgc atggcattgg tagccgagac cgcagagtgt gtggaagctg
      841 ccgagcgcat tgtccagttt gtgcaatccc gaatctgatt aaccgaaaag acctccatga
      901 gccaaccatt gcaacaaatc atcgaagccg cgtgggaaga ccgcgccaac atctccgtag
      961 cctctgcccc tgccgaagtg cgcgatgccg tggaacatgt gttgaacgaa ctcgacgccg
     1021 gccgccagcg cgtggccacg cgtgagggtg ttggccagtg gaccacgcac cagtggatca
     1081 agaaggccgt gctgctgtcc ttccgcctga aagacaacga aatcatgcaa gccggcagcc
     1141 tgggctttta cgacaaggtc gccaccaagt tctccaacct gagcgcagaa gaaatgaagg
     1201 ccaccggtgt gcgcgtcgtg ccgccggccg tggcgcgccg tggcagttac caggccaagg
     1261 gcgtgatcct gatgccctcg tactgcaaca tcggctctta tgtggacgaa ggctccatgg
     1321 tggacgcatg ggctaccgtg ggctcctgcg cccagatcgg caagaacgtg cacctgtctg
     1381 gtggtgtcgg tattggcggc gtgctcgaac ccatgcaggc aggccccacc atcatcgagg
     1441 acaactgctt cattggcgca cgctcggaaa tcgtggaagg cgtcatcatc gaagaaaact
     1501 cggtgatctc catgggcgtc tacatcggcc agagcaccaa gatctatgac cgcgccaccg
     1561 gcgaggtgtt ctatggccgc gtgccatccg gctcggtcgt ggtgtcgggc aacctgccca
     1621 gcgcagacgg caagtacagc ctgtactgcg ccgtcatcgt caagcgcgtg gacgcacaga
     1681 cccgtgccaa gacctcgctg aacgatttgc tgcgcgactg aatcaccccc actctggagt
     1741 ctgaacacga tggccaccaa cagcacaccg ggcacgatgg agcggattct ccgcctcatg
     1801 agtgacaaga aggcatccga tgtctacctg tcggcgcact cacctgcgct gatccggatc
     1861 aatggccagt gcgttccgat caacagccag ctgctgccgg ccgatgcccc gaagaacctg
     1921 ctggccgaag tcctgccggc agagcggatt gccgagctgg aggccaaagg tgagctcaac
     1981 atgggctttc cggtggacgg tgtggggcgt tttcgcgtca gcgccatgcg ccagcgcggc
     2041 tcctatgctg cggtgatccg ctacatcacc gtggatattc ctccactggc caatctgtcg
     2101 gttcccatga tgctgggcga cctgatcatg gagaaacgcg gtctgatcct gatggtgggt
     2161 gccaccggcg ccggcaagag caccacgctg gcgtccatgc tcgacttccg caatgagcat
     2221 ctggcaggtc acatcctgac cattgaagac ccgatcgaat tcctgttcaa gaacaagaag
     2281 gcggtggtca accagcggga aatcggcacc gacacgcagt cgctggagat tgcactgaag
     2341 aatgcgctgc gccaggcgcc cgatgtgatt ctgattggcg aaatccgtga ccgtgagacc
     2401 atgtccgcag ccattgccta tgcgcagtcc ggccatttgt gcctggccac catgcacgcc
     2461 aacaacagct accaggccct gaaccggatc ctgagcttct acccggtgga agttcgctcc
     2521 accatgctgg gcgatctggc ggctgcgctc aaggcggtgg tttctcagcg cttgctgcgc
     2581 accacgactg gtgcgcgtac gccggcggtg gaagttctgc tcaacaccaa gctcatttcc
     2641 gagctggtag aaaaaggcga cttctcgggc atcaaggaag ccatggagaa atccatggcg
     2701 gaaggctcgc agtccttcga gcacgacatc gcccgcctga tcgtcgacgg cacggtggac
     2761 cgcaaggaag gcctggccca tgcagactcg cccaccaacc tgatgtggcg cctgcaaaac
     2821 gacttcacca aggcggccca acccgttgcg gcgcaggaag aggacgaaca ggcctcgttc
     2881 acagaaatca cactggacgt gaagcattga tgatcgccgc gcagaactgc ttcaagcaag
     2941 atcgcgcccc ctcggggggc agtgcagcca acgcagtggc cagcgtgggg gcggtatgac
     3001 cgagactttg tacctggcgg agcagctgat tgcgcgcgcc tctgtcacgc cgctcgatgg
     3061 tggatgccag gagctgattg caaagcggct gactgcctgc ggttttgtct gcgaaagcat
     3121 cgtgagtggc ccggaggact ttcgggtcac caatttatgg gcaaaacggg ctgtagcccg
     3181 cgtcgatatt gcgcaagtag ctattaattc aatagcaaat acattgccag tcaaaacact
     3241 ggtctttgcc ggccacaccg atgtggtgcc caccggaccg ctggaccagt ggaccagccc
     3301 gccgtttacc cccagccacc gcgacggcaa gctctatggc cgcggtgcgg cggacatgaa
     3361 aacctcgctg gccgcttttg tggtggcggt agaagaattt atcgccgccc atccacagcc
     3421 gcaactcaac atcggctttc tgctgaccag cgacgaagaa ggcccggcca acgatggcac
     3481 ggtggtggtg tgcaacacgc tgaaggcgcg cggcgaagtg ctggactatt gcatcgtggg
     3541 agaacccacc tcggtcgagc gcactggcga catgatcaaa aacggccggc gcggcaccat
     3601 gagcggcaag ctcacagtca agggggtgca agggcacatt gcctacccgc acatggcccg
     3661 caaccccatc cacctggtgg ctcctgcact ggcggagttg gtgggagtgc agtgggacga
     3721 tggcaatgcg ttttttccac ccaccacctg gcaggtcagc aacatccatg gcggcacggg
     3781 ggcgagc
//
LOCUS       DAOSJM010000239         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21267_length_3784_cov_3.366854, whole genome shotgun sequence.
ACCESSION   DAOSJM010000239 DAOSJM010000000
VERSION     DAOSJM010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21267_length_3784_cov_3.366854"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PKX82_08920"
     CDS             <1..449
                     /locus_tag="PKX82_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52415.1"
                     /translation="LGLAFQGPSVMAVLLCAVDLVWRRTGSGRFREGPSFWSGAWLAA
                     GVLLGWALLLDTLALLPVSLYAWGFNAAVPALLVLLALLPWVLQGGSARQHSMSVLVV
                     IVLGVYVALHLPTGHVWDALLDPWLWLYLNVRAVRAGFAGRRGVLP"
     gene            complement(466..1488)
                     /locus_tag="PKX82_08925"
     CDS             complement(466..1488)
                     /locus_tag="PKX82_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOF52416.1"
                     /translation="MLNDILQSRHFRRLAYGSVLLLIITVIGTLGYHEIGSPNATWVD
                     SFYMTFITIATIGYGEIVDLSQHPMGRLFTVFISVLGIGTLSYLFSMVVALLIDADLN
                     AGLRKKRMEKEIAGLSDHYVICGIGRVGSNVATELLKTKRPFVVIESDREALDNWLEH
                     HPKTLHLHDDAADDDALHKAGAGRAAGVFAVTGDDSHNLMVALSVKMSDPKVRVVVRL
                     HDIRNTKKARRAGADEIVSPDFTGGMRIASAMVRPHVVNFMDKMLHSDEGLRVEEVIV
                     PNGFDPTPLATLIPKSRDYLLMAIQENGKWLFNPADDHPVQGGATLVVMANPEGRAHI
                     EKLVRA"
     gene            complement(1518..3296)
                     /locus_tag="PKX82_08930"
     CDS             complement(1518..3296)
                     /locus_tag="PKX82_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52417.1"
                     /translation="MTPPRSTIAVPWLWLVLVSAAALLYVLGLGSFYAPTNGDEMVYI
                     HIARMTAESGHWLPLQSEIVDTRNTKPPLLFWQAIAAGNWGHNWSLVALRLPSIAYTF
                     VTTGLLAFFAHRISGQWRTACVAAVLYLLFFSTFRYGRVYLTSAPETFWLALPMLWML
                     WQPLRNTPPGVQQAPAAPQLGFVAYTLFGMAMGLGAAYKSFALVAPAAAALWCAILVN
                     SMPLRWPVVIRTTLGVTWSTLMGLGIFALWFVLDPDPASVWQEFVVAENAGKMSGGQG
                     YWHAALFGAYPMWTQLLAYPENGGLLFFAVLGFLLLGLPRAFKSQTYVQMTPAMRILL
                     VWLVVWLVIFTIPSQRSARYVIPAMPALAIVMALLWDRIPRVWFWVTAAITVPALVML
                     ARIAWVMGDMEIASRSSNAITLIAVCAGLTGAAVTFFYKSWARTGALVTCLSVYATFG
                     LMVAPLEDARLQYSPQVQSAMRGARIAVPNGFTGQFERFRFVFPDSRLVPYDAEGRNT
                     GALHPELPPGERLQYLLREFDAVVWLQENGADTAPTCVPACRVIGQRWHVKSRHKGGE
                     ITLDNLWQPQQWLFRREWLVVGAQKP"
     gene            complement(3293..>3784)
                     /locus_tag="PKX82_08935"
     CDS             complement(3293..>3784)
                     /locus_tag="PKX82_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOF52418.1"
                     /translation="MSAPNTVQRATLWALGLLERYRYIKFGIVGASGTVVNLLVLHFG
                     HEYLFNQIEAGYNKPYFSLALAITLATLNNFTWNRLWTWADRVRTLEAGEVHPVSLRL
                     LGMEFGQYVTASAFGSALQYVLTLLLSNTMDYRLANIIAILAASVSNFLANDRWTFKR
                     HKR"
BASE COUNT          720 a         1138 c         1230 g          696 t
ORIGIN      
        1 ggctggggct ggctttccag gggcccagtg tgatggcggt gctgctgtgc gctgtggacc
       61 tggtctggcg ccggactggt tctgggcgtt tccgggaagg gccaagcttc tggagtggtg
      121 catggctggc ggcgggcgtg ttgctgggct gggcactgtt gctggatacc ctggcgttac
      181 tccccgtgtc gctgtacgcg tgggggttca acgctgcagt gccggcgttg ctggtgttgc
      241 tggccctgct gccctgggtg ttgcagggcg ggtcggcgcg ccagcattcc atgtcggtgc
      301 tggtggtcat tgttctcggt gtgtatgtgg ctctgcacct tcccacgggc catgtctggg
      361 atgccctgct ggacccctgg ctctggctgt atctgaacgt gcgcgctgtg cgagcaggct
      421 tcgccgggcg gcgtggcgtg ttgccctgac gcgaagcggg acgggtcagg ccctgaccag
      481 cttctcgatg tgggcgcgcc cttcagggtt ggccatcacc accagcgtgg cgccgccctg
      541 cacggggtgg tcgtccgccg gattgaacag ccatttgccg ttttcctgaa tcgccatgag
      601 cagatagtcg cgggacttgg gaatcagcgt ggccagtggc gtggggtcga aaccattggg
      661 cacaatcact tcctccacgc gcagcccttc atcgctgtgc agcatcttgt ccatgaagtt
      721 gacgacatgg ggacgcacca tggcagaggc aatgcgcatg ccaccggtga agtcggggga
      781 gacgatctcg tcggcgccag cgcggcgcgc tttcttggtg ttgcgaatgt cgtgcaggcg
      841 caccaccacg cggactttgg gatcgctcat tttgaccgac agcgccacca tcaggttgtg
      901 gctgtcatca ccggtgacgg caaatacgcc ggcggcgcgc ccggctccgg ccttgtgcag
      961 ggcgtcgtca tcggcggcat cgtcgtgcag gtgcagggtt ttggggtggt gctccagcca
     1021 gttatcgagc gcttcgcggt cactttcaat gacgacgaaa gggcgtttgg tcttgagcag
     1081 ctccgtggcg acattggagc ccacgcggcc aatgccacag atgacgtaat ggtccgagag
     1141 tccggcaatt tctttttcca tgcgtttctt tctcaatcct gcgttcaggt cagcatcaat
     1201 cagcagcgcc accaccatgg agaacaggta gctcagggta ccaattccca gcacggaaat
     1261 aaacactgtg aacagccgcc ccatcgggtg ctgcgacagg tccacgatct cgccatagcc
     1321 aatggtggca atggtgatga aggtcatgta gaagctgtcc acccacgtcg cgttggggga
     1381 gccgatctcg tggtagccca gggtgccgat gacagtgatg atcagcaaca gcaccgagcc
     1441 gtacgcgagt ctccggaagt gtctgctttg aagaatgtcg ttgagcacgt ccggtagttc
     1501 ctgcgtctgc gcttaagtta aggtttctgt gccccgacca ccagccactc ccgccggaac
     1561 agccactgct ggggctgcca gaggttgtcc agagtgattt cgccaccctt gtgccggctc
     1621 ttgacgtgcc accgctggcc aatgacccgg caggctggta cgcaggtcgg ggctgtatcc
     1681 gccccattct cctgcaacca gacaaccgca tcaaattcac gcaacagata ttgcagtcgc
     1741 tcgccaggcg gcagctcagg atgcagtgca ccggtgttgc ggccttctgc gtcgtagggc
     1801 accagccggc tgtcgggaaa cacgaagcgg aagcgctcga actggccggt gaatccattg
     1861 ggcactgcaa tgcgtgcgcc ccgcatcgca ctttgcactt gcgggctgta ttgcaagcgc
     1921 gcatcttcca gtggtgccac catcaggccg aaggtggcgt acaccgagag gcaggttacc
     1981 agagcacctg tgcgagccca tgatttatag aaaaaagtga ctgcagcccc cgtcaatcct
     2041 gcgcagacag ctatcaaagt aatagcattc gaggagcgtg aggcaatttc catgtcaccc
     2101 atcacccagg caatgcgggc cagcatcacc agcgcaggca ccgtgatggc cgctgtgacc
     2161 caaaaccaga cacggggaat gcggtcccaa agcaaggcca tgacaatcgc cagcgccggc
     2221 atggccggga tcacgtagcg ggccgagcgc tggctgggga tggtgaagat gaccagccac
     2281 acgaccagcc agaccagcag gatgcgcatg gccggtgtca tctgcacata ggtctggctt
     2341 ttgaaagcgc gtggcagccc gagcagcagg aaccccagta ccgcaaagaa cagcagtccg
     2401 ccattttccg ggtaggccag caactgggtc cacatggggt aggcaccgaa cagcgcggca
     2461 tgccagtagc cctgcccccc cgacatcttg ccggcgttct cagccaccac gaactcctgc
     2521 cagacggagg cagggtccgg gtcgagcaca aaccacagcg caaaaatgcc caggcccatc
     2581 aacgtgctcc aggtcacgcc cagcgtggtg cggatcacca cgggccagcg caggggcatg
     2641 ctgttcacca gaatggcgca ccacaatgcc gccgctgccg gcgcaaccag cgcaaaggat
     2701 ttgtaggccg cacccaggcc catggccatg ccgaacagcg tgtaggccac gaagccaagc
     2761 tgcggcgcag ccggtgcctg ctgcactcct ggaggtgtgt tgcgcaacgg ttgccagagc
     2821 atccagagca tcggcagtgc cagccagaag gtttctggtg ccgaggtcag gtacacccgg
     2881 ccgtagcgga aagtggagaa aaacagcagg tacagcacag cggcgacaca ggcggtgcgc
     2941 cattgcccgc tgatgcggtg ggcaaagaac gccagcaggc cggtggtgac aaaggtgtaa
     3001 gcgatgctgg gcaggcgcaa cgccaccagg ctccagttgt gcccccaatt gcctgcagca
     3061 attgcctgcc agaacagcag cggtggcttg gtgttgcggg tatccacgat ctcggactgc
     3121 aacggcagcc agtggcccga ctccgccgtc atgcgggcga tgtggatgta gaccatctca
     3181 tcgccgttgg tgggcgcgta gaagctgccc aggcccagca catagagcaa tgcagccgca
     3241 ctcaccagca ccagccagag ccagggcacc gcgatggtgg agcgtggggg cgtcatctct
     3301 tgtggcgctt gaaagtccag cggtcgttgg ccaggaagtt gctcacgctg gccgcgagga
     3361 tggcaatgat gttggccagc cggtagtcca tggtgttgga gagcagcagt gtcaggacgt
     3421 actgcagcgc actgccaaag gccgaggccg tgacgtactg gccgaactcc atgcccagca
     3481 ggcgcaaact gaccgggtgg acctcgccag cttccagggt acgcacccgg tcggcccacg
     3541 tccacagccg gttccaggtg aagttgttca gcgtggctag ggtgatggcc agcgccaggg
     3601 agaagtaggg tttgttgtag ccggcttcga tctggttgaa caggtactcg tgcccgaagt
     3661 gcagcaccag caggttcacc acggtgccgc tggcccccac gatgccgaac ttgatatagc
     3721 ggtagcgctc cagcaagccc agcgcccaca gcgtggcgcg ctgcacggta ttgggggcac
     3781 tcat
//
LOCUS       DAOSJM010000240         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21309_length_3779_cov_4.401719, whole genome shotgun sequence.
ACCESSION   DAOSJM010000240 DAOSJM010000000
VERSION     DAOSJM010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21309_length_3779_cov_4.401719"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..693
                     /locus_tag="PKX82_08940"
     CDS             310..693
                     /locus_tag="PKX82_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52419.1"
                     /translation="MLKKSLIATALVISTFGAFAQAPATPATPATPATPAVAATAAKP
                     ADAAKPADVAKPADVAKQKREEVRKQKEEARKAKHEAAKAKRAEAKAKLDAAKAKPAD
                     AKPAEVKPADMKAAAPAKAEMPAKQ"
     gene            complement(799..1785)
                     /locus_tag="PKX82_08945"
     CDS             complement(799..1785)
                     /locus_tag="PKX82_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HOF52420.1"
                     /translation="MKKIFVLGGTGFVGGHVCEKLVRAGWHVTVPTRRMVNARPVQHL
                     PGLTVLQLDVHNEAALAQAMAGHDAVVNLVAILHGDAAAFDKTHVILPQKVQRACRTA
                     AVQHLVHISALGANDERPGSAPSEYLRSKGRGEAVLLHPAEPHGALNITILRPSVIFG
                     ADDKFLNLFAKLQAVFPFMPLAGATARFQPVWVEDVAEAVVRSLQVPRSAASPLILEA
                     CGPEIYTLQQLVQLAARLGGVNDGRGRPVIALPDWLGRLQARMMELAPGAPLMSRDNL
                     DSMKVDNVATGTLPGLRALGIQPALLQPIAADYLSRGGKSHGLLGVRSRNRT"
     gene            complement(1824..2321)
                     /locus_tag="PKX82_08950"
     CDS             complement(1824..2321)
                     /locus_tag="PKX82_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52421.1"
                     /translation="MKHKASLWDGDYGTSRHITDVHWEGDITQEELISVADQISSDWQ
                     TIDLELDPAHPCHGNTYRLQKSWPNLLDEAYALRTLEAEDELEGTFVWVHCCKRVYDD
                     AGELEDEGGRTYRQVFNTVMFCLEFVAFDDIAAQCDEYFGPDGWDEYDLYLDDDLPEP
                     IAYCQ"
     gene            complement(2382..3287)
                     /locus_tag="PKX82_08955"
     CDS             complement(2382..3287)
                     /locus_tag="PKX82_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF52422.1"
                     /translation="MAAPGRRRTAHCLTDTGQPVGAFLWMSGALVSFCLMAVGARELA
                     GGISTFQILLIRSLIGLAMVTLLLVRTRRLGLFQTHRFRLHLGRNVFHFFGQYGWFLG
                     IGLLPLAQVFALEFTTPLWTLVVASLFLRESFTTRKVVAIALGFAGVCLILNPGRDIV
                     SPAALYVIGAAVCYAVAHVSTKALSTTDDALTILFYMCSVQLPLGLLFSWGNWVVPSP
                     MQWVWLVLIGVTALTAHFCITHAMKLADASVVVTLDFLRLPLIAGVGVAFYNEAFQPM
                     LLIGALLMLAGNLLNVYRPKSAAPG"
     gene            complement(3248..3694)
                     /locus_tag="PKX82_08960"
     CDS             complement(3248..3694)
                     /locus_tag="PKX82_08960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOF52423.1"
                     /translation="MKLVVPENKTLMHTMTTAIRWGDMDAMGHVNNTVYLRYMESARI
                     QLMSEGGFDTNPQGEGFVIANIFCNFIRQLEYPGDVVVRSYVGATGRSSFDLYHELLR
                     TDGGDTVYANGGATMVWVDFPKQQSLPMPERLRQWLHQAGGVPHTA"
BASE COUNT          795 a         1192 c         1149 g          643 t
ORIGIN      
        1 gaggctggcc acgttgtcca gcgctgcgcg cacaggctcg cctccggcgg tggtgatggg
       61 ggcatcggtc tccgggcggc gagacagggc tttgacggtg tggccgcgtg ccacgagttc
      121 gcgcagcaca tgcccaccca gaaagccgct ggcgccagtg agaaaaacga tagccatgaa
      181 aaagctcctt cggtggggtg gtgcgtacag cacggcactt tacattcgtt tacacggcct
      241 gcgtaaacgc agggacatca gcgcgagtta taggtacatg gcaacatcgc catgacctga
      301 aatactgaaa tgctcaaaaa atcgcttatc gctactgctc tggttatttc cacctttggt
      361 gcttttgccc aggcgccggc gactcctgcg acacccgcta cacctgctac cccagcggtt
      421 gctgcaacgg cagccaagcc tgctgatgct gcaaagcccg cagatgtagc aaagcccgca
      481 gatgtagcaa agcaaaaacg cgaagaagtt cgcaagcaga aagaagaagc tcgcaaggcc
      541 aagcacgaag ccgccaaagc caagcgcgct gaagccaaag ccaagctcga cgctgccaag
      601 gccaagcctg cggatgcaaa gcctgctgaa gtcaagcctg ccgacatgaa ggcagcagca
      661 cctgccaagg ctgaaatgcc tgccaagcag taatcactgc gccgctgcct gacggcagcg
      721 aaaacaaaac agccaccgcg ttccggtggc ttttttgctt tggcgacggc aacttctgga
      781 tcgcgtatgc cagccaggtc acgtgcgatt gcggctgcgg acgcccagca agccatggga
      841 cttcccgcca cggctgaggt aatccgctgc aatgggctgc agcagcgcgg gctggatgcc
      901 cagtgcgcgc aaaccgggca acgtgcctgt ggccacgttg tccaccttca tcgagtcgag
      961 attgtcccgg ctcatcagcg gtgcgccagg tgccagctcc atcatgcggg cctgcaggcg
     1021 ccccagccag tccgggagcg caatcaccgg gcggccccgc ccgtcattca ccccacccaa
     1081 acgcgcggcc aactgcacca gctgctggag ggtgtaaatc tccggaccgc aggcctccag
     1141 aatcagcggg gaagccgcag aacggggcac ctgcaggcta cgcaccacgg cttcggccac
     1201 gtcttctacc cagaccggct gaaaccgcgc agtagcgccg gccaatggca tgaacggaaa
     1261 cacggcctgc agtttggcga acaggttgag aaacttgtcg tccgcgccaa agatgacact
     1321 ggggcgcagg atggtgatgt tcagcgcacc atgcggctcg gcgggatgca acagaacggc
     1381 ttcgccccgg cctttgctgc gcagatattc cgatggggcc gatccggggc gctcatcatt
     1441 cgcgcccagt gcgctgatgt gcaccaggtg ctgcacggct gccgtgcggc atgccctctg
     1501 caccttctgg ggaagaatga catgggtctt gtcaaacgcg gctgcatccc catgcaggat
     1561 ggccaccagg ttcaccaccg catcgtgccc ggccatggcc tgcgcaagcg cggcctcgtt
     1621 atgcacatcc agttgcagca ctgtcagccc gggcagatgc tgcaccggtc gggcattgac
     1681 cattcgccgc gttggcacgg tgacatgcca gccagcgcgc accagttttt cacatacgtg
     1741 accgcccaca aacccggtac cgcccagaac aaagattttt ttcatgcggt cactgtagcg
     1801 ggtcacggga atccgcgcac ggcctactgg caataggcga tcggctcggg caggtcgtca
     1861 tcgaggtaca ggtcgtattc gtcccagccg tccgggccaa agtactcatc gcattgcgcg
     1921 gcgatgtcgt caaacgcgac gaactccagg cagaacatga cggtgttgaa cacctggcgg
     1981 taggtgcggc cgccctcatc ttcgagttcg cccgcgtcgt cgtacacgcg cttgcagcag
     2041 tgcacccaga caaaggtgcc ttcgagctcg tcttcggctt ccagcgtgcg cagcgcatag
     2101 gcctcgtcca gcaggttggg ccaggacttc tgcaaacggt aggtgttgcc gtggcaaggg
     2161 tgcgccgggt ccagttccag gtcgatggtc tgccagtcgg acgaaatctg gtcggccacg
     2221 gagatcagct cctcctgtgt aatgtcgccc tcccaatgca catcggtgat gtggcgggaa
     2281 gtgccgtagt cgccatccca gaggctggct ttgtgtttca tggcttcagg gtgcatggcg
     2341 atctccgtgg ttgcggttgc aactctaccg caaccgcggg atcaacccgg cgcagcggac
     2401 ttgggccggt agacgttgag caggttaccc gccagcatca gcagcgctcc gatgagcagc
     2461 atgggctgga acgcctcgtt gtaaaaggcc acgcccacgc cggcaatcag gggcaagcgc
     2521 agaaagtcca gtgtcaccac cacactggca tcggccagct tcatggcgtg ggtgatgcag
     2581 aagtgggcgg tcagcgccgt cacgccaatc aggaccagcc agacccactg catgggagaa
     2641 ggcaccaccc agttgcccca gctgaaaagc agccccagcg gtagctgaac cgagcacatg
     2701 tagaacagga tggtcagtgc atcgtcggtc gtagacagcg ccttggtgga cacgtgcgcc
     2761 acggcgtaac acacggcagc cccgatcacg tacagcgcgg ccgggctgac gatgtcgcgc
     2821 cccgggttca ggatgaggca cacgcccgca aaccccaatg caatggccac gaccttgcgg
     2881 gtggtgaagg actcccgcag aaacagcgag gccaccacca gcgtccacag cggcgtggtg
     2941 aactccagtg caaatacctg cgccagtggc aacaagccga tacccaggaa ccagccgtac
     3001 tggccaaaga aatggaacac gttgcgcccc agatggagcc tgaagcgatg ggtttgaaag
     3061 agcccaaggc gcctggtgcg gaccagcagc agcgtgacca tggccagccc gatcaggctg
     3121 cggatcagca ggatctggaa ggtgctgatg ccgccggcca gttcgcgggc gcccactgcc
     3181 atcaggcaga aggacaccag ggcgcccgac atccagagaa atgcacccac cggttggccc
     3241 gtatccgtca ggcagtgtgc ggtacgccgc cggcctggtg cagccattgg cgcagccgct
     3301 cgggcatggg cagcgactgc tgcttgggaa agtccaccca caccatggtg gcgccgccat
     3361 tggcatacac cgtatcgcca ccatcggtgc gcagcagctc gtggtacaga tcgaacgaag
     3421 aacgacctgt ggcgcccaca tagctcctga ccaccacatc accgggatac tccagctgcc
     3481 ggatgaagtt gcagaagatg ttggcaatca cgaagccttc gccctgcggg ttggtatcaa
     3541 acccgccttc gctcatcagc tgaatgcggg ccgactccat gtagcgcagg tacaccgtgt
     3601 tgttcacgtg ccccatggcg tccatatcac cccagcggat ggctgtggtc atggtgtgca
     3661 tgagcgtctt gttttcgggt actaccagtt tcattttgca aaaggctccg ttttaaacca
     3721 ctacaggctc gggctccagc aggatgccaa agcgttcgta cacgctggtc tggatggcc
//
LOCUS       DAOSJM010000241         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21758_length_3719_cov_4.108624, whole genome shotgun sequence.
ACCESSION   DAOSJM010000241 DAOSJM010000000
VERSION     DAOSJM010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21758_length_3719_cov_4.108624"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKX82_08965"
     CDS             <1..432
                     /locus_tag="PKX82_08965"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HOF52424.1"
                     /translation="PQARALNMQNQAAFPPAVTASSRNVVMPSPDMLYSVCTYDVSEG
                     PVRVTANPALPSYWSIALYASNSDNFYVLNDRKAGGKPVDLWLVSEGANRTDHAVPAG
                     SQVVVTPTRKGFLLMRVLTGNYEAEKATLEPARRTLACTKI"
     gene            441..1340
                     /locus_tag="PKX82_08970"
     CDS             441..1340
                     /locus_tag="PKX82_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOF52425.1"
                     /translation="MLAQLLRNLLLAQVLIGAALGYWLGQPEPLPLATMCLVAMGLPF
                     ATMILVDTVSALRSRAAEPWSLWWRSLFGEYVAGIVIFLFRQPWTRKVPTVLPATSQT
                     PQIPVVLIHGYMCNHRIWDDVSDVLRQKGHTVIAVNLEPVYASIDRYAPIVEAAVNDL
                     CRITGAQQVALVGHSMGGLAIRAWMRAHGTTRVARALTLGTPHQGTQMAKGVHSPNGK
                     QMAWKSRWLADLAATETDATRSLFRIAITPQDNIVFPQREQRMPGVPVQVFEGLGHLQ
                     MCLDPNVIHWVTQQLSDLPHARH"
     gene            1327..>1723
                     /locus_tag="PKX82_08975"
     CDS             1327..>1723
                     /locus_tag="PKX82_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52426.1"
                     /translation="MRAIESAALPSNLRSYASIFWHRRAGLKDPAAMPRIERSINAVQ
                     LNATWLNAYRRCVGLEAATASGLPPLALQIAVAPLHLSILADAQFPFKALGLVHVSQQ
                     VSQMAAIAPDQRLTLRAYTTQAQWARRGMT"
     assembly_gap    1724..1733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1734..2266
                     /locus_tag="PKX82_08980"
     CDS             <1734..2266
                     /locus_tag="PKX82_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOF52427.1"
                     /translation="FGLVTEAWADGQLVWRGETTALASGPSPLQATTNAKSAEAADQS
                     GHPWQQILALDAPEPLGRRYAAIAGDLNPIHQHALLARPFGFRQAIIHGTWTLARALA
                     AAQLPQTSPYTLNAQFRKPVSLPSSILVWSRRNDQQDEIKVTDKDGQTPHVTAQITRE
                     PTPHLYGRHPAVPSST"
     gene            2277..3098
                     /locus_tag="PKX82_08985"
     CDS             2277..3098
                     /locus_tag="PKX82_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF52428.1"
                     /translation="MTRIKRLKASLTQFPDIPAPSGWLLASEPRVFWEYVAGLTMFAT
                     VKRMAPRGDGHPVLVIPGLGASDTSTAMLRKLLDELGYKTYPWALGRNRGLKEHVTAA
                     MQARLQHIHDKHQQKVSIIGQSLGGVFARELAKLAPDAVRQVITLGSPFTGHPLASTG
                     THLYEWLSGDNLEDMDFNQHLEIRIKPPVPTTSVYSKLDGVVAWQCSIEKDQPGGENI
                     HLRGNTHCGMAFSPNALYLVAERLAQPDEDWKAFEPRWLGKVLYGTKDHRKAAAH"
     gene            3120..>3719
                     /locus_tag="PKX82_08990"
     CDS             3120..>3719
                     /locus_tag="PKX82_08990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOF52429.1"
                     /translation="MTDLVVRKLLIDLTAPFPARWNGGDAFRSAFFNALSMSFPLGEQ
                     YFIDSVRAGIKALPQADQDRLATEVQGFIGQEATHRRLHGLFNGNLEKLGFFNQLERR
                     ATKRLKDNAHRDVRVHVAATAATEHLTAIFAEWMLSHPEALQGTEPRLQTLWLWHSAE
                     ESEHRSTAFDVYTALGGSQEWRLRLFRYITVTFLSDITRQ"
BASE COUNT          726 a         1279 c         1058 g          646 t
ORIGIN      
        1 ccccaggcgc gggcactgaa tatgcaaaac caggccgcgt tcccgccggc cgtcaccgcc
       61 agctcgcgca atgtggtcat gcccagcccg gacatgctgt attcagtctg cacctacgac
      121 gtatcggaag gtccggtccg cgtgacggcc aacccggcgt tgccctctta ctggtccatc
      181 gccctgtacg cgtccaacag cgacaacttc tatgtcctca acgaccgcaa ggcaggaggc
      241 aaaccggtgg acctctggct ggtgtccgaa ggggccaacc gcaccgacca cgccgtaccc
      301 gcaggcagcc aagtcgtggt cacgcccacc cgcaaaggct ttctgctcat gcgtgtgctg
      361 accggcaact acgaagccga aaaagccacc ctcgaacccg cacgccgtac cttggcctgt
      421 accaagattt aggaaacccc atgctcgctc aactactgcg caacctgctg ctggcccaag
      481 tcctgatcgg tgccgcgctc ggctactggc tgggccagcc agagccgttg ccactggcca
      541 ccatgtgtct ggtcgcgatg ggcctgccat ttgccaccat gatcctcgtc gacaccgtca
      601 gcgccctgcg gtcacgtgcg gcagagccct ggtcgctttg gtggcggtcg ctgtttggcg
      661 aatatgtcgc gggcatcgtc atcttcctgt ttcgccagcc ctggacacgc aaggtaccca
      721 cggtattgcc agctaccagc caaacgccgc agataccggt ggtgctgatc catggctaca
      781 tgtgcaacca ccgcatctgg gacgatgtgt ctgacgtact gcgccagaag ggtcacaccg
      841 tcatcgccgt caacctcgaa ccagtgtatg cgtccattga ccgctacgcg cccatcgtag
      901 aagcagctgt aaacgacctg tgcagaatca ccggcgcgca gcaggtggcg ctggtgggcc
      961 acagcatggg cgggctggcc atccgcgcct ggatgcgcgc gcatggcacc acccgggtgg
     1021 cacgtgccct cacattgggc acgccccacc agggaacgca gatggcaaaa ggtgtgcatt
     1081 cgcccaatgg caagcagatg gcctggaaaa gccgctggct ggcagacctt gccgccacgg
     1141 aaaccgacgc cacccgctcc ctgttccgca ttgccatcac cccacaggac aacatcgtct
     1201 ttccccagcg cgaacagcgg atgccgggcg tgccggtgca ggtgtttgag ggtttgggcc
     1261 atctgcagat gtgcctggat ccgaacgtca tccattgggt cacccaacaa ctgagcgacc
     1321 tgccccatgc gcgccattga gtccgccgcc ctgccctcca acctgcgctc gtacgccagt
     1381 atcttctggc accgccgcgc cgggctgaaa gaccccgctg ccatgccgcg catcgaacgc
     1441 agcatcaacg cggtgcaact gaacgccacc tggctcaacg cctaccgccg ctgtgtgggc
     1501 ctggaggccg caaccgcttc cgggctaccg ccattggcgt tgcagattgc ggttgcccca
     1561 ctgcatcttt ccatcctcgc cgatgcgcag tttcccttca aggccctggg tctggtgcac
     1621 gtgtcacagc aggtcagcca aatggccgcc attgcaccag accagcgatt gacattgcgg
     1681 gcctacacca cgcaggcgca gtgggcacgc cgtggcatga cctnnnnnnn nnncctttgg
     1741 cctggtgacg gaggcttggg ccgacgggca actggtctgg cgcggtgaaa ccaccgcgct
     1801 ggcatccggt ccatcccccc tccaggcaac gaccaacgcg aaatcagcgg aggccgcaga
     1861 ccagtccggc catccctggc aacagattct ggcgctggac gcgcccgaac ccctggggcg
     1921 ccgctacgca gccattgcgg gcgatctcaa ccccatccac cagcatgcgc tgctggcgcg
     1981 tccctttggc tttcgacagg ccatcatcca tggcacctgg acactggcac gcgccttggc
     2041 ggcggcacaa ctcccgcaga cttcgccgta caccctgaat gcgcagtttc gcaaaccggt
     2101 cagcctgcct tccagcatcc tggtgtggtc gcgcagaaac gaccagcagg acgaaatcaa
     2161 ggtcaccgac aaggacggcc aaacgcccca tgtcactgca cagatcacgc gtgaaccgac
     2221 accccacctc tacggacggc acccagccgt tccctcttcg acctgaagga attgatatga
     2281 cacgcatcaa gcgactcaaa gcctcgttga cacagtttcc cgatattccg gctccctcgg
     2341 gctggctgct ggcatccgag ccacgggtct tctgggagta cgtagccggg cttaccatgt
     2401 ttgccaccgt caagcgcatg gcgccgcgcg gagacgggca ccccgtactc gtgatccccg
     2461 gcctcggtgc atccgataca tcgaccgcta tgctgcgcaa gttgctggat gagctgggct
     2521 acaagaccta tccctgggca ctgggccgca accgtggcct caaggaacac gtgacagcgg
     2581 ccatgcaggc gcgcctgcag cacatccatg acaagcacca gcagaaggtc agcatcattg
     2641 gccagagtct gggcggcgtg ttcgcgcgcg aactggccaa gttggcgccc gacgccgtgc
     2701 gccaggtcat tacgttgggt tcaccgttca ccggccaccc gctggcatcg accggcacac
     2761 acctgtacga atggctgtcg ggcgacaacc tggaagacat ggatttcaac cagcatctgg
     2821 aaatccgcat caagcctccg gtacccacca cctcggtcta cagcaagctc gacggtgtgg
     2881 tggcatggca atgttccatc gaaaaagacc agcccggagg tgaaaatatc catctgcggg
     2941 gcaacaccca ctgtggcatg gcattcagcc ccaacgccct gtacctggtg gcagagcgcc
     3001 tggcacaacc cgacgaagac tggaaggcat ttgagcccag gtggctggga aaagtcctgt
     3061 atggcacgaa agaccaccgc aaggctgcgg cccactgatt tgcatctgga gacattgcca
     3121 tgactgacct ggtagttcgc aaacttctga ttgacctgac cgccccgttc ccggcacgct
     3181 ggaacggtgg cgatgccttc cgctccgcct tcttcaacgc gctgtcgatg agtttcccgc
     3241 tgggtgagca gtacttcatt gattcggtgc gcgccggcat caaggcactc ccgcaggcag
     3301 accaggatcg tctggccacc gaggtacagg ggtttatcgg ccaggaggcc acccaccgca
     3361 ggctgcacgg cctcttcaac gggaacctgg agaagctggg ttttttcaac cagctggagc
     3421 gccgtgccac taagcgcctg aaagacaacg cgcaccgcga tgtccgcgtc catgtagcgg
     3481 cgaccgccgc cacagaacac ctcaccgcca tctttgccga gtggatgctc agccaccccg
     3541 aggcactgca gggcacggag ccccgcttgc aaacgctgtg gctgtggcat tcggcagaag
     3601 agtccgagca ccgcagcacc gcattcgatg tgtacaccgc tctgggcggc tcgcaggaat
     3661 ggcgcctccg gctgttccgg tacatcaccg tgaccttcct ttcggacatc acacgccag
//
LOCUS       DAOSJM010000242         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21767_length_3718_cov_3.907999, whole genome shotgun sequence.
ACCESSION   DAOSJM010000242 DAOSJM010000000
VERSION     DAOSJM010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21767_length_3718_cov_3.907999"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..942
                     /locus_tag="PKX82_08995"
     CDS             67..942
                     /locus_tag="PKX82_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF52430.1"
                     /translation="MLTRNAPENIALSAHSVRASLGNGPAKTAVLHDVSLDIVAGQWT
                     SIVGPNGAGKSTLLKVLAGVLHHGGTVRLLGRDLHRIAHRERARQLAWLGQNEASADD
                     LTVWDVAMLGRLPHQDWLSAPSAADHAAVEQALRATQAWDWRCRALGQLSGGERQRVL
                     LARALAVQAQVLLMDEPLANLDPPHQADWLAVVQALVAQGTTVVSVLHEISMALHADQ
                     VVVMAQGRVTHHGASGDPATHEALVNVFDRRIAIHAIEGQWVALPKTNPSREDRNGCN
                     THPGLLGVGSLLPLR"
     gene            950..1519
                     /gene="cobO"
                     /locus_tag="PKX82_09000"
     CDS             950..1519
                     /gene="cobO"
                     /locus_tag="PKX82_09000"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOF52431.1"
                     /translation="MHIETPPKEKPYEKAEGERRGIVIVNTGDGKGKSTAAFGLALRA
                     HGRGKKVKIFQFMKVPTARFGEHRMFEQIGIPIEGLGDGFSWKSQDLEHSAQLARDGW
                     AKAKAAILSGDYFMVTLDEITYPLIYGWMPLDDVLETLKTRPSHVHVVLTGRRCPPEI
                     IEIADTVSDIQMVKHAFKAGIPAQRGIED"
     gene            1680..2732
                     /gene="bioB"
                     /locus_tag="PKX82_09005"
     CDS             1680..2732
                     /gene="bioB"
                     /locus_tag="PKX82_09005"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HOF52432.1"
                     /translation="MSATLTPCATDARAEQPVHFHAPHKSPKARPAGDQRVWSVEEVL
                     ALLEMPFNDLLFQAQTVHRAHFDPNLVELATLLSVKTGGCPEDCGYCPQSVHFDTGVE
                     AGKLMGVDAVREAAERAKAAGATRFCMGAAWRAPKDRDVEAVAELVKAVKATGLEACA
                     TLGMLNDGHAETLRDAGLDYYNHNLDSAPDFYGDIITTRDYQDRLDTLARVRNAGVSV
                     CCGGIVGLGESRLQRAGLIAELANLAPYPESVPINHLVKVAGTPLADQPDLDPLEFVR
                     TIAVARITMPLARVRLSAGRQSMGDAVQALCFAAGANSIFYGDRLLTTANPDGDADMA
                     LLARLGLRAGQPVPRP"
     gene            2738..3706
                     /gene="cbiB"
                     /locus_tag="PKX82_09010"
     CDS             2738..3706
                     /gene="cbiB"
                     /locus_tag="PKX82_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOF52433.1"
                     /translation="MAFALALLVALAIDRWWGEPRARVHPVVWIGNYLGWVATWVQHG
                     TQPQAAKQDLPAFWRGAVAWCVGAAWVAALAWVLQIALLALPWWAAGIGLGVLLKPLL
                     AWAMLRDEVRAVESALGKSLQAGRERLRWLVSRDVAQLSATEVRESAIETLAENLNDS
                     VVAPIFWFVLLGLPGVALYRFANTADAMWGYPGVYKGRNWAWAGKWAARADDVLSWLP
                     ARITAVLLLLAAGWNGLSRAGTLAQEAGKTPSPNSGWPMAAMALNLGVALRKPGVYVL
                     NPQGKPASAAHTALALLFASKALLALVVCAQTAMILVAFFSKRITP"
BASE COUNT          630 a         1180 c         1278 g          630 t
ORIGIN      
        1 gggcggcggc tacctgctgt ggctgatgca ccgcggccag gcgcacgaag gagggcgggc
       61 atgaccatgc tgacccgaaa tgctcctgaa aatatagcgc tgtccgccca ttccgtgcgg
      121 gccagccttg gaaatggtcc tgcaaagacg gcggtgctcc acgatgtctc actggacatt
      181 gttgcggggc agtggaccag catcgtcggc cccaatggcg cgggtaagtc tacgctgctc
      241 aaggtgctgg ccggggtgct gcaccacggc ggaaccgtgc gcctgctggg gcgggacctg
      301 caccgcattg cgcaccgcga acgcgcgcgc cagctcgcct ggctggggca aaacgaagcg
      361 tctgccgatg atttgacggt gtgggacgtg gcgatgttgg gccgcctgcc gcaccaggac
      421 tggctgagcg caccgagtgc agccgaccat gcggccgtgg aacaggccct gcgcgctacg
      481 caggcctggg actggcgttg tcgcgcgctg ggtcagctca gcggcggcga gcgccagcgc
      541 gtgctgctgg cacgcgcgct ggccgtgcag gcgcaggtgc tgctgatgga cgagccgctg
      601 gccaacctgg acccgccaca ccaggccgac tggctggcgg tggtgcaagc cctggtggcg
      661 cagggcacga ccgtagtcag cgtgttgcac gagatcagca tggcgttgca tgcggaccag
      721 gtggtcgtca tggcgcaggg gcgcgtgacc caccacggtg ccagtggcga tccggcgacc
      781 catgaggcgc tggtcaatgt attcgaccga cgcattgcca tccacgccat tgaagggcag
      841 tgggtggcat tgccgaagac caacccttcg agggaggacc ggaatgggtg taacacccac
      901 cccggcctcc tcggagtcgg ttcactcttg ccgcttcgtt agaaaaccta tgcacatcga
      961 aacacccccc aaagagaaac cctacgaaaa ggccgagggc gagcgccgcg gcattgtcat
     1021 cgtcaacacc ggagacggca agggcaaaag cacggccgct ttcggtctgg ccctgcgcgc
     1081 ccacggccgc ggcaagaagg tcaagatttt ccagttcatg aaagtgccca ccgcccgctt
     1141 tggcgagcac cgcatgttcg agcagattgg cattcccatc gaaggactgg gcgacggctt
     1201 cagctggaag agccaggacc tggagcattc ggcgcaactg gcgcgcgacg gctgggccaa
     1261 ggccaaggcc gccatcctga gtggcgacta cttcatggtg acgctggacg aaatcaccta
     1321 cccgctgatc tacggctgga tgccgctgga cgatgtactc gaaaccctca agacccggcc
     1381 cagccatgtg catgtggtac tgactggccg gcgctgcccg cccgagatca tcgagattgc
     1441 ggacaccgtc agcgacatcc agatggtcaa acacgcgttc aaggccggca ttccggcgca
     1501 gcgcggcatc gaagattgac catcgaaagc caaagtgggt gcaaatcatg ccgttttgtt
     1561 caagttgcga cctcaggctc atggcgatgc acgtcagcct ggcggtgcgg ccacagaatg
     1621 cgcccggtca cgccgtatgg gcgggcccca cgttttgaat cattaaggag attgatccca
     1681 tgtctgccac tttgacccct tgcgccacgg acgcccgcgc ggagcagccc gtgcatttcc
     1741 atgcgcccca taaatcgccc aaagcccgcc ctgcaggtga ccagcgggtc tggtccgtag
     1801 aggaagtgct cgcgctgctg gagatgccgt tcaatgatct gctgttccag gcgcagacgg
     1861 tgcaccgtgc ccactttgat cccaatctgg ttgagctggc cacgctgttg tcggtcaaga
     1921 ccggtggctg cccggaggac tgcggctatt gcccgcagtc ggtgcacttt gacaccggtg
     1981 ttgaggctgg caagctcatg ggggtggacg cggtgcgcga agccgccgag cgcgccaagg
     2041 cggcgggagc cacgcgcttt tgtatgggtg ccgcctggcg tgcaccgaag gaccgcgatg
     2101 tggaggcggt ggccgagctg gtcaaggccg tcaaggccac cgggctggag gcctgcgcca
     2161 cgctgggcat gttgaacgac ggccacgccg aaaccctgcg cgacgccgga ctcgattact
     2221 acaaccacaa cctcgacagc gcgccggatt tctacggcga catcatcacc acgcgcgact
     2281 accaggaccg gctcgatacg ctggcacggg tacgcaatgc gggcgtcagc gtctgctgcg
     2341 gcggcattgt gggcctgggc gaatcgcgcc tgcagcgcgc cggcctgatc gccgagctgg
     2401 ccaatctggc gccgtacccc gagtcagtgc cgatcaacca cctggtcaag gtggcgggca
     2461 caccgctggc ggaccagccc gatctggacc cgctggaatt tgtgcgcacc atcgccgtgg
     2521 cgcgcatcac catgccgctg gcacgcgtgc gcctgtcagc tggtcggcaa agcatgggcg
     2581 acgcggtgca ggcgctgtgt tttgccgccg gtgccaactc catcttctac ggtgacaggc
     2641 tgctcaccac ggccaacccc gatggcgatg ccgatatggc cctgctggca cgcctgggcc
     2701 tgcgcgccgg gcagccggtg ccgcgcccct gacgacgatg gccttcgcac tcgcgctcct
     2761 cgtggccctg gcgatcgatc gctggtgggg cgagccgcgg gcgcgggtgc atccggtggt
     2821 gtggatcggc aactacctgg gctgggtcgc cacctgggtg caacatggca cgcaaccgca
     2881 agctgcgaag caggacctgc ccgcgttctg gcgcggcgcg gtggcctggt gcgtcggggc
     2941 cgcctgggtg gccgctctgg cgtgggtgct gcagattgcg ctgctggcct tgccctggtg
     3001 ggcagcgggc atcgggttgg gcgtgctgct caagccgctg ctggcctggg ccatgctgcg
     3061 ggatgaagtg cgtgccgtgg agtcggcatt gggtaaatcc ctgcaggccg ggcgcgagcg
     3121 cctgcgctgg ctggtgagcc gcgacgtggc acagctcagt gcgactgaag tgcgcgagag
     3181 cgccattgag accctggccg agaacctcaa cgactcggtg gtggcgccga ttttctggtt
     3241 cgtgctgctg ggcctgccgg gcgtggcgct gtaccggttt gccaacacgg ccgatgccat
     3301 gtggggctat ccaggcgtgt acaagggacg caactgggcc tgggccggca aatgggctgc
     3361 acgcgcagac gatgtgctga gctggctgcc cgcgcgcatc acggccgtgt tgctgttgct
     3421 ggcggccggc tggaacggcc tgtcgcgggc aggtacattg gcgcaggagg cgggcaagac
     3481 accgtccccc aacagcggct ggccgatggc cgccatggcg ctgaacctgg gcgtggcctt
     3541 gcgcaaaccc ggtgtctatg tgctcaatcc gcagggcaag cctgccagcg ctgcgcacac
     3601 cgcgctggcc ctgctttttg catcaaaagc gctgctagcc ctcgtggtat gtgcgcagac
     3661 agctatgatt ttggtagcat ttttcagcaa gagaatcaca ccatgagcgc gcccctgc
//
LOCUS       DAOSJM010000243         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21906_length_3701_cov_5.655513, whole genome shotgun sequence.
ACCESSION   DAOSJM010000243 DAOSJM010000000
VERSION     DAOSJM010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21906_length_3701_cov_5.655513"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..419
                     /locus_tag="PKX82_09015"
     CDS             72..419
                     /locus_tag="PKX82_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52434.1"
                     /translation="MQFLFDFVGQVMRSVLRLVLLLAAGVFALSLLTAVLVAVLLTAL
                     WSLLTGRKPAMFTTFTRFRQASRQFRQGPWSPQGGAAGGGAPPADVVDVQAHEVDERT
                     VRAHLPLDPGARN"
     gene            complement(424..1449)
                     /locus_tag="PKX82_09020"
     CDS             complement(424..1449)
                     /locus_tag="PKX82_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOF52435.1"
                     /translation="MTTPHTILIVAEAADTLSLMQETFKESCLVQVASSDQQALEMAS
                     TNPVPALIFLQADMPGMDGFALIQQLRSEPGTAAIPVLAISAADAFLKDQDDFLEQEV
                     ARRTRDILAVQDATILAMAALAEIRDADTGNHTLRIQHYVRALARKLSRHPRFASGLT
                     PQNITLLFKSTPLHDIGKVGIPDRILLKPGKLTPEEFEIMKTHTTVGRQVIDKAEQAL
                     QQSVPLFAYAKEIAYSHHEKWDGTGYPERLAGEQIPLSARLMALADVYDALISRRVYK
                     EPTGHEQAVEIIRQTTGRHFDPDVVDAFLEIQDTFKTIAIAYSDEKSDLQKKADYIAM
                     VNSLQPD"
     gene            complement(1478..2335)
                     /locus_tag="PKX82_09025"
     CDS             complement(1478..2335)
                     /locus_tag="PKX82_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF52436.1"
                     /translation="MTDRFSRAFPFLFVVLWSTGFIGARLGLPHIEPLTFLLIRYLVV
                     IGCMVVLALAFRAPWPQTPSAWLHIGIAGLLMHGVYLGGVFIAISKGLPAGVASLVVG
                     VQPLLTAVGAGWLLGETVLKRQWLGLLLGLAGVALVVFNKIGGTFGLAALVPAVAALI
                     GITAGTLYQKRFCPSFDWRTGSVAQFVPTAVVTGLLAWCFETGTIDWTGELVFALGWL
                     VLVLSLGAVSLLNWLIRNSNAVNIASLFYLVPPCTAVFAWLLFGETFTGLALLGMVLT
                     VWGVYLARK"
     gene            complement(2332..>3701)
                     /gene="priA"
                     /locus_tag="PKX82_09030"
     CDS             complement(2332..>3701)
                     /gene="priA"
                     /locus_tag="PKX82_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOF52437.1"
                     /translation="EHDPSYKSSEGARYSARDLAVYRGRLESAKVLLGSATPSLESWH
                     LSRPAALGGRYLRLLMPSRVGASGVPSQPADAAATTQQAMRAQSGTPTGLPLVRRVDM
                     NHQPRNAVFSLPLIAAIKERVARGEQCMVFLNRRGYAPVLHCADCGWKSECPHCSAFR
                     VFHKIDRTLRCHHCGFTERVPRACPSCGNPDITPIGRGTEQLEEHLAELLGDHVRIAR
                     IDADSTRLKGALEEQLAAVHAGDVDVLVGTQMIAKGHDFRRITLVAAVNPDGALFSSD
                     FRAPERLFSLLMQAAGRSGRDAALGAQAEVWIQTFQPAHPLYAALKAHDYPAFAAQQL
                     KEREQAALPPFSAQALVRADARTQEAAQAFLNLARNGALTDMAQWEGWSEVLEQITLY
                     PAVPMTIQRIANIERAQMLIESPSRSALQQFLTGWHSVLHTVRPQPECKGLIRWAIDV
                     DPLAI"
BASE COUNT          662 a         1147 c         1213 g          679 t
ORIGIN      
        1 cgccgtggcg ctgatgaccg gaaagtagac gcgacttttc tggggccaag cgccgtgtgc
       61 acaatgtgcg catgcaattc ttatttgatt tcgtgggcca ggtgatgcgc agcgtgctgc
      121 ggctggtgct gcttctggct gccggcgtat tcgcgttgag cctgctgacg gcggttctgg
      181 tggccgtgtt gctgaccgca ctgtggtccc tgctcactgg ccgcaagccg gccatgttca
      241 ccaccttcac ccgttttcgc caggcttccc ggcaatttcg ccagggaccc tggtcacccc
      301 agggtggggc tgcaggtggt ggcgcaccgc cagccgatgt ggtggatgtg caggcccatg
      361 aggtggatga acgcaccgtg cgtgcgcatc tgccgctgga tcccggggcc aggaactagc
      421 cgctcagtcc ggctgcaggg aattcaccat ggcgatgtag tcggccttct tctgaaggtc
      481 tgacttctcg tcggagtacg cgatggcgat ggtcttgaat gtgtcctgga tttcaagaaa
      541 ggcgtcgaca acatcgggat caaaatgacg tcctgtggtc tgccggatga tttccaccgc
      601 ctgttcgtgt cccgtgggtt ccttgtagac gcgcctgctg atcagcgcat cgtacacatc
      661 ggccaatgcc attaaccgcg cagacagggg gatttgctcg cccgccagtc tctccgggta
      721 gcctgtgcca tcccattttt catggtgcga gtaggcgatt tccttggcat aggcaaagag
      781 cgggacggac tgctgcagtg cctgctccgc cttgtcaatt acctgacgcc ccaccgtggt
      841 gtgcgtcttc atgatctcga attcctcggg tgtcagcttg cccggtttga gaagaatgcg
      901 atccggtatt cccactttgc caatgtcgtg caggggagtt gacttgaaca ggagggtaat
      961 gttctgcggg gtcagtccgc tggcgaagcg cggatgcctg gagagttttc tggccagtgc
     1021 ccggacgtag tgctgaatgc gcagcgtgtg gtttcccgta tcggcgtccc ggatctcggc
     1081 caatgccgcc atggccagga tggtggcatc ctggacagcc aggatatcgc gcgtgcgcct
     1141 ggccacttcc tgctccagaa aatcgtcctg gtccttgaga aaggcgtcgg cggcggaaat
     1201 ggcaaggacc ggaatcgccg cagtaccagg ttcgctcctg agctgctgga taagcgcaaa
     1261 gccgtccatt cctggcatat cagcctgcag gaagatgagg gccggcacgg ggttggtgct
     1321 ggccatttcc agcgcctgct ggtcgctgga tgcgacctgg accaggcagg actccttgaa
     1381 ggtctcctgc atcagggaca acgtgtccgc tgcttcggca acaatcagaa tggtgtgtgg
     1441 ggtcgtcata tcacggtcga tgcatccata gcgcatatta cttccgcgcc agatacaccc
     1501 cccacaccgt gagcaccatg cccaagagcg cgaggcccgt aaaggtttcg ccaaacagca
     1561 gccaggcaaa cacggcggtg cacggcggca ccaggtagaa caggctggcg atgttgaccg
     1621 cgttgctgtt gcggatcagc cagttcagca agctcaccgc acccagcgag agcaccagca
     1681 ccagccagcc cagggcaaag acaagttcac cggtccagtc gatcgtgccg gtctcgaagc
     1741 accaggccag cagtccggtc acgacagcgg taggcacaaa ctgcgccacc gagccggtgc
     1801 gccagtcaaa cgacgggcaa aagcgtttct ggtacagcgt gccggcggtg ataccgatca
     1861 gcgcggcgac ggccggcacc agtgcggcga ggccaaaggt gccgccaatc ttgttgaaca
     1921 ccaccagcgc cacgccggcc aggcccagca gcagacccag ccactggcgc ttgagcactg
     1981 tttcgcccag cagccatcct gcgcccacgg cggtcagcaa tggctgcaca cccaccacca
     2041 ggctggccac acccgcgggc agccccttgg agatggcgat gaacacaccg cccaggtaca
     2101 cgccgtgcat caagaggccc gcgatgccga tatgcagcca ggcgctgggt gtctgcggcc
     2161 agggcgcgcg aaacgccaac gccagcacca ccatgcagcc gatgaccacc aggtagcgaa
     2221 tcagcagaaa ggtcagcggc tcaatgtgcg gcagtcccag ccgggcgccg ataaagccgg
     2281 tggaccacag cacgacaaac agaaacggaa aggcacggga aaaacgatcg gtcatatggc
     2341 aagggggtct acatcaatag cccagcgtat caggcctttg cattcgggtt gtgggcgcac
     2401 ggtgtgcaat acgctgtgcc agccggtcag aaactgctgt agtgcgctgc gcgagggact
     2461 ctcgatgagc atctgcgcac gctcgatgtt ggcgatgcgc tggatggtca tgggcaccgc
     2521 cgggtacagc gtgatctgct ccagcacctc gctccagccc tcccactgcg ccatgtcggt
     2581 gagtgcgccg ttgcgtgcca ggttcaggaa ggcctgcgca gcctcctggg tgcgggcgtc
     2641 tgcgcgcacc agcgcctggg cgctgaaggg aggcagcgcg gcctgctcgc gctccttgag
     2701 ttgttgtgct gcaaaggcgg ggtaatcgtg ggccttgagt gcggcgtaca gcgggtgtgc
     2761 gggctggaaa gtctggatcc acacctcggc ctgtgcgccc agtgctgcgt cacgtcccga
     2821 gcggcccgcg gcctgcatca gcaggctgaa caggcgctcc ggcgcccgga agtcgctgga
     2881 gaacagcgcg ccgtccggat tgaccgcggc caccagcgtg atgcggcgaa agtcgtggcc
     2941 cttggcaatc atctgcgtgc ccaccagcac atctacgtcg cccgcatgta cggcggccag
     3001 ttgctcttcc agagcacctt tgaggcgggt ggaatcggca tcaatgcggg caatgcgcac
     3061 gtggtcaccc agcaactcgg ccaggtgttc ttcgagctgt tcggtacccc ggccgatggg
     3121 cgtgatgtcg gggttgccac agctggggca ggcgcgcggc acccgttcgg taaaaccgca
     3181 gtggtggcag cgcagcgtgc ggtcaatctt gtggaacacg cggaacgcac tgcagtgcgg
     3241 gcattcgctc ttccagccac agtccgcgca gtgcaatacc ggtgcatagc cgcgccggtt
     3301 cagaaagacc atgcactgct cgccgcgcgc cacgcgctcc ttgatggccg cgatcagcgg
     3361 cagcgaaaac accgcattgc ggggctggtg gttcatgtcc acccggcgca ccagcggcag
     3421 gccggtgggt gtcccggatt gggcgcgcat ggcttgctgg gttgttgctg ccgcgtcagc
     3481 gggttgggac ggtacgccac tggcgccaac gcggctgggc atcagcaggc gcaggtagcg
     3541 ccctcccagt gccgcggggc ggctcaggtg ccagctctcc agcgaggggg tggccgatcc
     3601 cagcagcacc ttggcgctct cgagccggcc gcggtacacc gccaggtcgc gggccgagta
     3661 gcgtgcgccc tcgctgcttt tgtagctggg gtcgtgttct t
//
LOCUS       DAOSJM010000244         3699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21924_length_3699_cov_3.963227, whole genome shotgun sequence.
ACCESSION   DAOSJM010000244 DAOSJM010000000
VERSION     DAOSJM010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21924_length_3699_cov_3.963227"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PKX82_09035"
     CDS             <1..752
                     /locus_tag="PKX82_09035"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOF52438.1"
                     /translation="PSTGLATGEGIYDVDGFMVPFFYAQPEGKKNLPVILVIQEIFGV
                     HEYIADTCRRFAQAGYLAIAPELYARQGDPSKYGEMAKLLAEVVSKVPDEQVMGDLDG
                     AVKWAGENGGNLKKVGITGFCWGGRITWLYAEHSKNVKAGVAWYGRLVGAPSALTPKH
                     PVQLAATLKAPVLGLYGGQDGGIPLTTVNEMKDALAAAKGNAAAKASEFVVYPDAPHA
                     FHADYRPSYRKDAAQDGFKRALEWFKAKGVA"
     gene            880..1683
                     /locus_tag="PKX82_09040"
     CDS             880..1683
                     /locus_tag="PKX82_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOF52439.1"
                     /translation="MTLIAILLGTLAAGIGSVWLAAALSFGALGRYTQHMLSLAAGAL
                     MATAFMHLLPEAFESQAGAHDLFMTLLIGVVFFFLLDKAELWHHGHEHHHAEEAPHDH
                     AHHHGHPHADTVTRVSGSWAVLTGDSVHCFGDGVLIASAFMADMRLGVIASLAVLAHE
                     VPHHMGDLMVLRSGSTNKRAALVKVSLAGAVTALGGLIGFWLVDQLQDFLPYFLVVAS
                     SSFIYVALADLIPQLQKHLSARETAAQVAWLVSGIGLVMLVSGLAHSGH"
     gene            complement(1697..2566)
                     /locus_tag="PKX82_09045"
     CDS             complement(1697..2566)
                     /locus_tag="PKX82_09045"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HOF52440.1"
                     /translation="MTYTTLISVAQLQALQASDAPAVVFDCSCDLMQPEQGPAQYAES
                     HIAGALYAHLDNNLSAKGDRAVTGAASGGRHPLPSREAFARWLGSMGLQNTDQVVVYD
                     RQGANYCGRLWWMLKWLGHANVAVLDGGFQAWQAAGGAVTSGAEAPRAGTTFAMGDPL
                     VTLVGADTVLHNLNKGTQHIIDARGAPRFRGEVEPIDPVAGHIPGALNRVFSSNLGPD
                     GLFKPAAVLRAEFEALLGQRQPQDVVHHCGSGVSAVPNILAMEIAGLGRTALFAGSWS
                     EWCSDPTRPVAQG"
     gene            2821..3171
                     /locus_tag="PKX82_09050"
     CDS             2821..3171
                     /locus_tag="PKX82_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52441.1"
                     /translation="MNLSQKSEADILSIANPLMDNLMDASTHVDYERHVRDFTDRLKA
                     QLPKERLESICREYQSTKGFFAGREFVKLFRRPDSVAIVWKQGFTKQVGEYVAEMVLV
                     EQDGKYLVDHVMVF"
     gene            complement(3226..>3699)
                     /locus_tag="PKX82_09055"
     CDS             complement(3226..>3699)
                     /locus_tag="PKX82_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF52442.1"
                     /translation="IDQNQLFAGVVGLLSGMGAALAYLQVTALGKAGEPEERTVFYFS
                     VGTTVVGSVGMLFTDITPWSAVRWQDAVWVIPIGVLASLGQWCMTRAYRKGATLVVAS
                     MQYSGIVFAVMFSLILFGDQIAPMGWIGIAVIVASGVLATVLRSRAMPNTPAEEH"
BASE COUNT          718 a         1107 c         1155 g          719 t
ORIGIN      
        1 caccgtccac tggcctggcc acgggggagg gcatctacga tgtggatggc tttatggtgc
       61 cattctttta cgcgcagcct gaaggcaaga agaacctgcc ggtcattctg gtgatccagg
      121 aaatttttgg cgtccacgaa tacatcgctg atacctgccg ccgctttgcc caggccggct
      181 acctggcgat cgcccccgag ttgtatgccc gtcagggtga cccatccaag tacggcgaaa
      241 tggccaagct gctggccgag gtcgtctcca aggtgccgga tgaacaggtc atgggcgatc
      301 tggatggtgc cgtcaaatgg gctggcgaaa acggcggcaa cctcaagaaa gtgggtatca
      361 ccggattctg ctggggtggc cgcattacct ggctgtatgc cgagcacagc aagaacgtca
      421 aggcgggtgt ggcctggtat ggccgcctgg tgggcgcacc ctcggccctg acgcccaagc
      481 atccggtaca actggccgcc acgttgaagg ctcctgtgct ggggctgtac ggcgggcagg
      541 acggcggtat tccgctgacc accgtgaatg agatgaaaga cgcgctggcg gctgccaagg
      601 gcaacgctgc ggcaaaagcc tcggagtttg tggtgtaccc cgatgcgccg catgccttcc
      661 atgccgacta ccgtcccagc taccgcaagg acgcagcgca agacggcttc aaacgtgccc
      721 tggaatggtt caaggccaag ggcgtggcct gatcccgggc cggtccgccg cactgcacag
      781 gccgccgcaa ggcggccttt ttattgtcag gcacaattcg tgcatggcaa ttgtgaatga
      841 aatcggcctc tggcccccga ggaaactgcg taagtagcta tgactttgat agcaatcttg
      901 ttaggaacgc tcgctgccgg cattggcagt gtctggctgg ctgccgcttt gagctttggt
      961 gcgctgggtc gttacacgca gcacatgctg agcctggccg ctggcgcgct gatggcgacc
     1021 gccttcatgc acctgttgcc cgaagccttc gagagccagg cgggcgcgca cgacctgttc
     1081 atgacgctgc tgatcggtgt ggtgttcttc ttcctgctgg acaaggccga gctgtggcac
     1141 cacggccatg agcaccacca tgccgaagaa gcaccgcacg accatgccca ccaccacgga
     1201 cacccgcacg cggacacggt aacgcgggtg tcgggaagct gggcggtgtt gacgggtgac
     1261 agcgtgcact gctttggtga cggtgtgctg atcgcttcgg cctttatggc agatatgcgt
     1321 ctgggcgtca ttgcctcgct ggccgtgctg gcccacgaag ttccgcacca catgggcgac
     1381 ctgatggtgc tgcgttcggg ctccaccaac aaacgcgcgg cgctggtcaa ggtgtcgctg
     1441 gccggcgcgg tcacggcgct gggcggattg atcggcttct ggctggtgga ccagctgcag
     1501 gactttctgc cgtattttct ggtggtggca tccagcagct ttatctatgt ggcactggca
     1561 gacctgatcc cccagttgca aaagcacctc agtgcgcgag agaccgcagc ccaggtggcc
     1621 tggctggtgt caggcattgg gctggtgatg ctggtcagcg gcttggccca cagcggccac
     1681 tgaactttcc ttcagactag ccctgtgcca ccgggcgtgt ggggtcactg caccactcac
     1741 tccaactgcc ggcaaacagg gcagtgcgac ccagaccggc aatttccatc gccagaatat
     1801 tgggcactgc gctgacgcca ctgccgcagt ggtgcaccac atcttggggc tggcgctggc
     1861 ccagcagtgc ctcaaactcg gcgcgcaaca cggcagcggg tttgaacagg ccgtccgggc
     1921 ccagattgct gctgaagacg cggttcaacg caccgggaat atggccagcc acggggtcga
     1981 tgggctccac ctcgccgcga aagcgtggcg cgccgcgggc atcgatgatg tgctgcgttc
     2041 ccttgttgag gttgtgtagc acggtgtctg cacccaccag tgtcaccagc gggtcaccca
     2101 tcgcaaaagt ggtgcccgca cgcggcgctt cggcaccgct ggtcacggcg cccccagcgg
     2161 cctgccaggc ctgaaatccg ccgtccagca ccgccacatt cgcatggccc agccacttga
     2221 gcatccacca caggcggccg cagtagttgg caccctggcg gtcgtagacc acgacctgat
     2281 ccgtgttctg caggcccatg ctgcccagcc agcgggcaaa agcctcgcgc gaaggcaggg
     2341 gatggcgtcc gccggatgca gcaccggtga ctgcgcgatc gcctttggcg ctcaggttgt
     2401 tgtcgaggtg tgcatacagg gccccagcaa tgtggctttc ggcgtactgc gctggccctt
     2461 gctcgggctg catcaggtcg caactgcaat cgaaaacaac ggccggcgca tcgctcgcct
     2521 ggagtgcttg cagctgggca acagaaatca gggtggtgta ggtcatggtg ttgcgattat
     2581 ctattcgctt ttgtgccgtt gcttgcgctc gctgggctat gtggtgtgga gccggtggcc
     2641 tcatcagtcc gctgcgcgtc cagcaaatcg gctaccggct acacgccaca tagccccggg
     2701 ggtgggtatg ctcacgcaat caaagatatc gtggttgacg agcgttatgc agcgggagtg
     2761 gatagacaga aagtgcatca aaatggcagg tgcaacccct tgcgaaaacg gtgctctcac
     2821 atgaacctat ctcaaaaatc agaagcagat attttgtcga tcgccaatcc actgatggac
     2881 aacttaatgg acgcttctac tcacgtcgat tacgaacggc acgtacggga ctttacagac
     2941 aggctcaaag cgcaattgcc caaagagcga ttggagagca tttgtcggga gtaccagtcc
     3001 accaaggggt tttttgccgg cagagagttc gtgaagctgt ttcgtcgccc cgactccgtt
     3061 gccatcgtat ggaagcaagg ctttaccaag caggtcggtg aatatgttgc agagatggtc
     3121 ttggttgagc aggacggcaa atacctcgtt gaccatgtga tggtttttta gcctttgaac
     3181 tgcctctttg gcgtcaggtg aaagtgagct tccagtctga aacggtcaat gttcttccgc
     3241 tggcgtgttg ggcatcgcac gagagcgtaa aaccgtggcc agaacgccgc tggccacaat
     3301 gacagcgatg ccgatccagc ccatgggtgc gatctggtcg ccgaacagaa tcaggctgaa
     3361 catcaccgca aacacaatgc ccgaatactg catgctggcc accaccaggg tggcgccctt
     3421 gcggtaggcg cgggtcatgc accactggcc cagggatgcc agcacgccaa tgggaatcac
     3481 ccacacggca tcctgccagc gcacggcgga ccagggtgtg atgtcggtga acagcatgcc
     3541 caccgaaccc acgacagtag tgcccacgga aaaatagaac acggtgcgct cttcgggctc
     3601 acccgccttg cccagcgccg tgacctgcag ataggccagt gcggcgccca tgccggagag
     3661 cagccccacc acgccggcaa acagctggtt ctggtcaat
//
LOCUS       DAOSJM010000245         3696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21948_length_3696_cov_4.744301, whole genome shotgun sequence.
ACCESSION   DAOSJM010000245 DAOSJM010000000
VERSION     DAOSJM010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21948_length_3696_cov_4.744301"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="PKX82_09060"
     CDS             complement(<1..457)
                     /locus_tag="PKX82_09060"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOF52443.1"
                     /translation="MGNRLTQIATRTGDNGTTGLGDNTRVSKNSLRVHAMGEVDELNS
                     HIGVLLCEDMPAEVRTQLVEIQHQLFNLGGELSIPGFELLKPEAVLALDEALEQHNAT
                     LPRLQEFILPAGTRAAALAHVCRTVARRAERAVVALGNEEALKETPRQYL"
     gene            507..1955
                     /locus_tag="PKX82_09065"
     CDS             507..1955
                     /locus_tag="PKX82_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOF52444.1"
                     /translation="MNAPAHHSHVQPPAQPDLLPEVHARNVPQALLDALKARFGDQYS
                     AAQAVREQHGRDESSYSVPPPSAVVFAQSTQDVADAVKLAAQYKVPVIPFGVGSSLEG
                     HLLAVQGGISLDVGRMNQVLSINAEDLTVTVQPGVTRMQLNAAVKSTGLFFPIDPGAD
                     ATIGGMSATRASGTNAVRYGTMRENVLSLEVVTASGDIIRTGTRAKKSSAGYDLTRLL
                     VGSEGTLGVITEVTVKLYPLPEAVMAATCSFGSLADAVNATIQIIQMGVPIARCELLD
                     STTVKVVNQHSKLNLPENAMLLFEFHGSPAGVKEQVETVQSITDEFGGAAFAWAETPE
                     ERTRLWTARHNAYFAGVQSVPGCKAITTDACVPISNLAAALLDSVTEAQEAGLPYFMV
                     GHVGDGNFHMGYLIDPDKPEERVLAEQLNTQLVARALKLGGTCTGEHGVGLHKMEFLV
                     TETGNGAVDMMRTIKRALDPDNIMNPGKIFML"
     gene            2036..2596
                     /locus_tag="PKX82_09070"
     CDS             2036..2596
                     /locus_tag="PKX82_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOF52445.1"
                     /translation="MIFWIILGVAVVYLVTLYNSLVTVKNGVSKAWANIDVLLKQRHD
                     ELPKLVDTCKQYMQHEQATLEKVIAARSQVSAAREAHNVGALGTAEGALRSGLGQLFA
                     LAENYPDLKANEQFLHLQSRISGLENAIADRREFYNESVNINNVAIEQFPGVLLARWF
                     SFKAFELLKFAAEELQDVDIGQRFKG"
     gene            2603..3508
                     /locus_tag="PKX82_09075"
     CDS             2603..3508
                     /locus_tag="PKX82_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52446.1"
                     /translation="MRVWALRALRSQNLSGLLVAAYIGLFTAALQLRGSPMARASLVA
                     AVVVGIVAWAGALRRARAVAHLATSRIASAAQGYVEVMGRASVDPDNLILSPMSGVQC
                     LWYRYRVFSRDNAKEEWRQTDSGVSSATFEIRDETGAARVDPDHAEVLGAEVRTNYPD
                     TDTKLVEELLFGGRVLYVLGEFSTIGGAHSVLSVRDDVSTLLAEWKTDAAGLKRRFDL
                     DGNGEVDLREWELARRLATKTVEQQHRAIRKEPGVHMLRAPPDGRLFLISALSPQTLR
                     RRFVLWSFAHLGLALAAAVLLVRWH"
BASE COUNT          676 a         1167 c         1197 g          656 t
ORIGIN      
        1 tgaggtactg gcgcggtgtt tccttgaggg cctcttcatt gcccagggcc accaccgcgc
       61 gttcggcgcg ccgggcgacg gtgcggcaca catgggccag cgcagcggca cgggtgccgg
      121 ccggcaggat gaattcctgc agtcggggca gagttgcgtt gtgttgttcc agcgcttcgt
      181 ccagcgccag caccgcctcg ggcttgagca gctcaaagcc gggaatggac agctcacccc
      241 ccaggttgaa cagctggtgc tggatctcca ccagctgcgt acgcacctcg gccggcatgt
      301 cttcgcacag cagcacgccg atatgcgagt tgagttcgtc cacttcgccc atggcgtgca
      361 cgcgcagact gttcttggaa acacgcgtgt tgtcccccag tcctgtggtg ccgttatctc
      421 cggtgcgggt ggcaatttgt gtcaggcgat ttcccatgga cagtcccttg gatgaaaatg
      481 cgattattcc ctgtttggag ctccccatga acgcccccgc ccaccactcc cacgtgcagc
      541 cccccgcgca acccgatttg ctgcccgaag tccacgcccg caatgtgcca caagccctgc
      601 tcgacgcact gaaggcgcgc tttggtgacc agtattctgc ggcgcaggcg gtgcgcgagc
      661 aacatggccg cgacgagtct tcctacagcg tgccgccgcc gtccgcggtg gtgtttgcgc
      721 agagcaccca ggacgtggcc gacgccgtca aactggcagc gcaatacaag gtgccggtga
      781 ttccctttgg cgtgggctcc tcgctggaag gccacctgct ggcggtacag ggcggcatca
      841 gcctggatgt gggccgcatg aaccaggtgc tgtccatcaa cgccgaagac ctgacggtca
      901 cggtgcaacc cggcgtgacg cgcatgcagc tcaatgcggc ggtcaagtcc actggcctgt
      961 tcttcccgat cgatcccggc gccgacgcga ccattggcgg catgagcgcc acgcgtgcca
     1021 gtggcaccaa cgccgtgcgc tacggcacca tgcgcgagaa cgtgctatcg ctggaagtgg
     1081 tgaccgccag cggcgacatc atccgcaccg gtacccgcgc caagaaatcg tcggcgggct
     1141 acgacctgac ccgcctgctg gtgggcagcg agggcacgct gggtgtgatc accgaagtca
     1201 ccgtcaagct gtatccgttg cccgaagccg tgatggccgc cacctgctcg tttggcagcc
     1261 tggccgatgc ggtgaacgcc accatccaga tcatccagat gggcgtaccc atcgcccgtt
     1321 gcgagttgct ggactcgacc accgtgaaag tggtcaacca gcactccaaa ctgaacctgc
     1381 ccgaaaacgc catgctgctg tttgaattcc acggctcacc cgctggcgtc aaagagcagg
     1441 tggaaaccgt gcagtccatc accgacgaat ttggcggcgc cgcctttgcc tgggccgaaa
     1501 cgcccgaaga gcgcacccgc ctgtggaccg cacggcacaa cgcctacttt gccggcgtgc
     1561 agtcggtgcc gggctgcaag gccatcacca ccgatgcctg cgtacccatc tcgaatctgg
     1621 ccgcagcgct gctggactcg gtgaccgaag cccaggaggc cggcctgccc tacttcatgg
     1681 tcggccacgt gggcgacggc aatttccaca tgggctacct gattgacccc gacaaacccg
     1741 aggaaagggt gctggccgag caactcaaca cgcagctggt ggcgcgtgca ctgaaactgg
     1801 gcggcacctg caccggtgaa cacggtgtgg gcctgcacaa gatggagttt ctggtgaccg
     1861 aaacgggcaa tggtgcggtg gacatgatgc gtaccatcaa gcgcgcactg gacccggaca
     1921 acatcatgaa tccgggcaaa atctttatgc tataaattca atagctgcct gcgtatattc
     1981 cgcgggggct ggaggccaat ttgactacat attcaaaggg agaaacagcg tgggcatgat
     2041 tttctggatc atcttgggcg tagcggtggt gtaccttgtc acgctgtaca acagcctggt
     2101 caccgtaaag aatggcgtgt ccaaagcctg ggccaatatc gatgtgctgc tcaagcagcg
     2161 ccatgacgag ttgcccaagc tggtggacac ctgcaagcag tacatgcagc acgaacaggc
     2221 cacgctggag aaagtgattg cagcccgctc gcaggtatcc gccgcgcgcg aggcccacaa
     2281 cgtgggggcg ctgggcacgg ccgagggcgc gctgcgcagt ggcctgggcc agctgtttgc
     2341 gctggccgaa aactaccccg acctcaaggc caacgagcag ttcctgcacc tgcagtcgcg
     2401 catcagcgga ctggagaatg cgattgccga ccggcgcgag ttctacaacg agagcgtgaa
     2461 catcaacaac gtggcgatcg agcagtttcc gggcgtgctg ctggcgcgct ggttcagctt
     2521 caaggcgttt gagctgctga agtttgccgc ggaagagctg caggacgtgg acatcggcca
     2581 gcgcttcaag ggctaggcac cgatgcgggt gtgggcactg cgggcactgc gcagccagaa
     2641 cctgtctggc ctgctggtgg cggcgtatat cggcctgttc acggcggcgc tgcaactgcg
     2701 cgggagcccc atggcccggg ccagcctggt ggcggccgtg gtggtgggta tcgtcgcgtg
     2761 ggccggtgcc ttgcgccggg cccgggcggt ggcgcacctg gccacgtcgc gcatcgcatc
     2821 ggcagcgcaa ggctacgtgg aggtgatggg ccgcgccagc gtggacccgg acaacctgat
     2881 cctcagcccc atgagtggtg tgcaatgcct ctggtaccgc taccgggtgt tttccagaga
     2941 caacgccaaa gaggagtggc gccagaccga cagcggggtg agcagcgcca ccttcgagat
     3001 ccgggatgaa accggcgcgg cccgggtgga ccccgaccac gccgaagtgc tgggcgccga
     3061 agtgcgcacc aactaccccg acaccgatac caaactggtc gaagaactgc tgttcggcgg
     3121 ccgcgtgctg tatgtgctgg gcgagttctc caccatcggc ggagcccatt ccgtgctgag
     3181 cgtgcgcgac gatgtcagca ccctgctggc ggaatggaaa accgacgctg ccggactcaa
     3241 gcgccgcttt gacctcgatg gcaatggaga ggtcgacctg cgcgagtggg aactggcgcg
     3301 gcggctggcc accaagaccg tggagcagca acaccgcgcg atccgcaagg agccgggggt
     3361 gcacatgctg cgtgcgccac cggatggccg gctgttcctg atttctgcgc tctcaccgca
     3421 aacattgcgt cggcgctttg tgctctggag ctttgcacac ctcggcctgg cgctggccgc
     3481 ggcggtgctg ctggtgcgct ggcactaggc ggccacgcca ggggccttac atttccgcgt
     3541 tttcggcgat ggcgttggcc aggtactcga tctttttctg catgtcggcg tcggtatccg
     3601 cgtacttctg cgcaatggcc gcaaattccg gactgacctc gatgaaggca tccaccacat
     3661 cggggtcgaa gtgggaggcg cgctcgctga agatca
//
LOCUS       DAOSJM010000246         3695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_21955_length_3695_cov_5.004121, whole genome shotgun sequence.
ACCESSION   DAOSJM010000246 DAOSJM010000000
VERSION     DAOSJM010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21955_length_3695_cov_5.004121"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1257
                     /gene="urtC"
                     /locus_tag="PKX82_09080"
     CDS             151..1257
                     /gene="urtC"
                     /locus_tag="PKX82_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HOF52447.1"
                     /translation="MIPTLILPSKAPLLTRRGWSAWLAALVVLCALAPVLNLWVPADS
                     VFHLSDFAVGLIAKIMCYAICALAMDLIWGYTGILSLGHGLFFALGGYAMGMYLMRQI
                     GLDGNYKSTLPDFMVFLDWKTLPWHWTFSDSFIATLFLVVAVPGLVALVFGYFAFRSR
                     IKGVYFSIITQAMTFAAMLLFFRNETGFGGNNGFTDFKRLLDIPIATPSMRMTLFVLT
                     ASTLLGFFLLARWLVNSKFGRVLQAVRDAESRVMFSGYSPLGYKLTIWTLSAMMCGVA
                     GALYVPQVGIINPSEMSPANSIEIAIWAAVGGRATLIGPIVGAFLVNGAKSWLTVTYP
                     EFWLYFLGALFIGVTLYLPDGVVGLVKKLKGGAK"
     gene            1340..2221
                     /gene="urtD"
                     /locus_tag="PKX82_09085"
     CDS             1340..2221
                     /gene="urtD"
                     /locus_tag="PKX82_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HOF52448.1"
                     /translation="MTPDLLEQGAKRIEARMAALAANTGQTESGGRNAGFSRVLTQGE
                     VDVTHGRILYLEDVHVSFDGFKAINGLSLDIAPGELRCIIGPNGAGKTTMMDIITGKT
                     RPNSGTVFFGSTIDLLRHSEPEIAQLGIGRKFQKPTVFEQLTVFENLELALHTDKGVK
                     SSMFFRLDSAQGDRLAEVLHTIHLADSVGRQAGNLSHGQKQWLEIGMLLMQEPKLLLL
                     DEPVAGMTDEETERTAELFLSLKGKHSLMVVEHDMSFIRTISEIVTVLCDGSVLAQGT
                     LDQVQSDERVIEVYLGR"
     gene            2224..2916
                     /gene="urtE"
                     /locus_tag="PKX82_09090"
     CDS             2224..2916
                     /gene="urtE"
                     /locus_tag="PKX82_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HOF52449.1"
                     /translation="MLNVKNINQYYGGSHILRDVSLQAELGKVTVILGRNGVGKTTLL
                     KSMMGLVPIKTGSIELDSKPIQQATPYERARAGVGFVPQGREIFGRLTVEENLRMGLA
                     YKSASTPIPPELFDLFPVLKQMLGRRGGDLSGGQQQQLAIARALAAKPRVLILDEPTE
                     GIQPSIIKDIGRVIRMLADRGDMAIVLVEQYYDFAQELADKYLVMERGAVVASGLGKD
                     MEANGVRKLVAI"
     gene            complement(2923..>3695)
                     /locus_tag="PKX82_09095"
     CDS             complement(2923..>3695)
                     /locus_tag="PKX82_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HOF52450.1"
                     /translation="RHQHTGPLRVLQSLYPEEDAICHNVLVHPPGGLVGGDRLDIRVR
                     AGSGAHGVITTPGATRFYRSDGATATQNSHLTLEPDARMEWLPLEALCYSGCLAENRL
                     TLDLAPGAELLGWDVTALGLPHADQPFVQGQFQQHIEVPGVWLERGNIAASDTRLLDG
                     PTGLAGQRCLASLFFVAGTPLTRTRREQALDSVREVIAQHALAPACGATSPHPQVVVV
                     RVMAPVVEPAMDLLRSLRNVWRTALWGIAPSNPRIWSM"
BASE COUNT          675 a         1120 c         1183 g          717 t
ORIGIN      
        1 gatcgccgta ctggtgttca tcatcatctt tatccagaag cgtccccaag gcatctttgc
       61 catgaagggc cggagcgcag aagcatgagc gccgcgccgg gccgtcccca agcaagctcg
      121 caccgcagcc cgcagggcga aggtatttca atgatcccta ccttgattct tccttcgaaa
      181 gcccctttgc tcacacgcag aggctggagc gcctggctgg ccgcgctggt ggtgttgtgt
      241 gccctggcgc cagtgctcaa cctgtgggta cctgccgaca gcgtgttcca cctgagcgac
      301 tttgcggtgg gactgatcgc caagatcatg tgctacgcca tctgcgcact ggccatggac
      361 ctgatctggg gctacaccgg aatcctgtcg ctgggccacg gcctgttctt tgcattgggc
      421 ggctacgcca tgggcatgta cctgatgcgc cagattggcc tggatggcaa ctacaaaagc
      481 accttgcccg acttcatggt gtttctggac tggaaaaccc tgccctggca ctggaccttc
      541 tcggacagct tcatcgccac gctgttcctg gtggtggccg tgccgggcct ggtggcgctg
      601 gtgtttggct actttgcttt ccgctctcgc atcaagggcg tgtacttctc catcatcacg
      661 caggccatga cctttgccgc catgctgctg ttcttccgca acgaaaccgg gttcggcggc
      721 aacaatggat ttaccgattt caaacgcctg ctcgacattc cgattgccac acccagcatg
      781 cgcatgacgc tgtttgtgct cacggccagc accttgctgg ggttctttct gctggcacgc
      841 tggctggtca attcgaagtt cggccgcgta ctgcaggcag tgcgcgatgc cgagtcgcgg
      901 gtcatgttca gcggctactc gcccctgggc tacaagctca ccatctggac cctctcggcc
      961 atgatgtgcg gcgtagccgg cgcgctgtat gtaccgcagg tgggcatcat caatcccagc
     1021 gaaatgagcc cggccaactc gatcgagatt gccatctggg ctgcggtcgg tgggcgtgcc
     1081 acgctgatcg ggcccatcgt gggcgccttt ctggtcaatg gcgccaagag ctggctgacc
     1141 gtgacttatc cggaattctg gttgtacttt ctgggggcgc tgttcatcgg cgtgacgctg
     1201 tacctgcctg atggggttgt ggggctggtc aagaagttaa agggaggtgc gaaatgaacg
     1261 acgccgggcc gccccaagtc gtgagggccc ccatgggggg cagcgcagca catgcaatgg
     1321 caagcgtggg ggctacttta tgactcccga tctattggag caaggcgcca agcgcatcga
     1381 ggcccgaatg gccgcgctgg ctgccaacac cgggcaaacc gagtcgggtg gtcgcaacgc
     1441 gggcttctcc cgcgtgctga cccagggcga agtagatgtg acccacgggc gcatcctcta
     1501 cctggaggat gtgcacgtca gctttgacgg cttcaaggcc atcaatggcc tgtcgctgga
     1561 cattgcaccg ggtgagttgc gctgcatcat tggtcccaac ggtgcaggca agaccaccat
     1621 gatggacatc atcacgggca agacacggcc caattccggc accgtgtttt tcggcagcac
     1681 cattgatctg ctgcgccaca gcgagcccga gattgcgcag ctgggcattg ggcgcaagtt
     1741 ccagaaaccc acggtgttcg agcagctcac ggtgttcgag aacctggagt tggcgttgca
     1801 taccgacaag ggggtgaagt cctccatgtt ttttcggctg gattcggcgc agggcgaccg
     1861 gctggcggag gtgctgcaca ccatccacct ggctgacagc gtgggccgcc aggcgggtaa
     1921 cctcagccat gggcagaagc agtggctcga aatcggcatg ctgctgatgc aggagcccaa
     1981 actcttgttg ctggacgagc ccgtggcggg catgaccgat gaggagaccg agcgcacggc
     2041 agagctgttc ctgtcactca agggcaagca ttccctgatg gtggtggagc atgacatgag
     2101 cttcatccgc accatcagcg aaatcgtcac cgtgctgtgc gatggttcgg tgctggcaca
     2161 gggcacgctg gaccaggtgc aaagcgatga gcgggtcatc gaagtctatc tgggacgctg
     2221 atcatgctca atgtaaaaaa catcaaccag tactacggcg gctcgcatat cctgcgcgac
     2281 gtcagcctgc aggccgagct tggcaaggtc acggtcatcc tggggcgcaa cggcgttggc
     2341 aagaccacgt tgctcaagtc gatgatgggt ctggtgccga tcaagacggg ctccatcgaa
     2401 ctcgatagca agcccatcca gcaggccacg ccgtacgagc gcgcgcgcgc cggtgtgggt
     2461 tttgtgccgc agggccgcga gattttcggc cgtctgacgg tggaagaaaa cttgcgcatg
     2521 ggcctggcct acaaatccgc cagcacgcca atacctcccg aactgtttga cctgtttccg
     2581 gtgctcaagc agatgctggg gcgccggggg ggtgacctgt ccggtgggca gcagcagcag
     2641 ttggccatcg cgcgtgcact tgccgccaag ccccgggtat tgattctcga cgaacccacc
     2701 gaaggcatcc agcccagcat catcaaggac atcggtcgcg tgatccgcat gctggcggat
     2761 cggggtgaca tggcgatcgt gctggtggag cagtactacg actttgcgca ggaactggcc
     2821 gacaagtacc tggtgatgga acgcggcgcg gtcgtggcca gcggcttggg caaggacatg
     2881 gaagccaacg gcgtgcgcaa gctggttgcc atctagtccc gcttacatcg accagatccg
     2941 gggattggac ggggcaatgc cccacaacgc cgtgcgccac acgttgcgca gcgagcgcag
     3001 caaatccatc gcgggttcca ctaccggtgc catcacgcgc accaccacca cttgcggatg
     3061 gggactggtg gcgccacagg cgggtgccaa cgcatgctgg gcgatcacct cgcgcacgct
     3121 gtccagcgcc tgttcccggc gggtgcgcgt gagcggtgta cctgccacga agaaaagcga
     3181 ggccaggcag cgctggccgg ccagccccgt agggccatcg agcaggcggg tgtcactggc
     3241 ggcaatgttg ccgcgttcca gccagacgcc aggcacttca atgtgttgct ggaactggcc
     3301 ttgcacaaac ggctgatctg cgtggggcaa ccccagggcc gtgacatccc agccgagcaa
     3361 ttctgcgcct ggggccaggt ccagggtcag ccgattttcg gccaggcagc cgctgtagca
     3421 cagcgcctct agaggcagcc attccatgcg tgcgtcgggt tccagcgtga ggtgcgaatt
     3481 ctgcgttgcg gtcgcgccgt ccgagcggta gaagcgtgtg gcgcccggcg tggtgatgac
     3541 gccgtgggca cccgagcctg cacgtaccct gatatccagt ctgtccccac ccaccagacc
     3601 tccgggcggg tgcaccagca cgttgtggca aatcgcatcc tcttcagggt acagactctg
     3661 cagcacgcgc agtgggcccg tatgctggtg gcggg
//
LOCUS       DAOSJM010000247         3678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_22104_length_3678_cov_3.642285, whole genome shotgun sequence.
ACCESSION   DAOSJM010000247 DAOSJM010000000
VERSION     DAOSJM010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22104_length_3678_cov_3.642285"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKX82_09100"
     CDS             <1..328
                     /locus_tag="PKX82_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HOF52451.1"
                     /translation="YLLPDMNFDPSESVFVPFYGVLAATIPSLSRFARLSRAKVVPVI
                     TRMTAGGYDVEILPAWQDFPTADAAADTALMNLRLQGYIDTMPSQYYWVHKRFKDRPS
                     GEAAPY"
     gene            complement(329..1249)
                     /locus_tag="PKX82_09105"
     CDS             complement(329..1249)
                     /locus_tag="PKX82_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF52452.1"
                     /translation="MMETWQQALPHGISLSCRAAGQRGRPVLVFLHGFPEGAFVWDAL
                     LTHFSRPENGGYRCVAPNLRGFEQSTAPAEVSAYRPKHLVQDIAALIDIEAGGDNAIA
                     ALIAHDWGGAVAWNLANQMPQRMEKLVIFNSPHPGTFLRELKNSPSQQEASAYMNFLV
                     QPGAEQHLAANDFARMWAFFTDMGNQRKADNWLTEDVKQQYRNVWSHGLQGGCNYYRA
                     SPLRPATAADPAASVVEIPASMLTIVVPTLVVWGMNDTALPPTLIEGLEAYVPDLTVR
                     KILDATHWIIHEQPELAIQLVQDYLSQRKG"
     gene            complement(1246..1956)
                     /gene="yihA"
                     /locus_tag="PKX82_09110"
     CDS             complement(1246..1956)
                     /gene="yihA"
                     /locus_tag="PKX82_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOF52453.1"
                     /translation="MTNSTNPPSKPPIAAASTPGEKSPATVALGWLHTARFLTTAPQL
                     HFLPELDVPEIAFVGRSNAGKSTCINTLTQQKQLAFASKKPGRTQHINLFALGKQGIT
                     DAVLTDLPGYGYAAVPKQDKIRWQQVMANYLVSRKNLQGIVLMCDPRHGMTELDEILL
                     DVIRPRVAEGLKFLVVLTKADKLTRSEGAKALSITKLQAGGGEVMLFSAPKRTGIEDV
                     AQLLWRWAHPEPSQPPAP"
     gene            2027..2656
                     /locus_tag="PKX82_09115"
     CDS             2027..2656
                     /locus_tag="PKX82_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOF52454.1"
                     /translation="MKLIAPLFIAAVVAVTSVSAFAAEAIPTVAKPDLAKGEASYGAV
                     CAACHGVDGNSGTPAYPKLSQQHPEYLVKQLQEFKSDKRSNAIMKGFASMLSDDDMKN
                     ISYWLATKKAKPGTAKDKELVTLGERIYRGGIADRQVPACAGCHSPNGAGIPAQYPRL
                     SGQHADYAVAQLTAFRDGVRKNSLQMTQIAAKMNDREIKAVADYVAGLR"
     gene            2767..>3678
                     /locus_tag="PKX82_09120"
     CDS             2767..>3678
                     /locus_tag="PKX82_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HOF52455.1"
                     /translation="MTVSTQGLELSSGSRAVKAAVELLSSMRFAISLLTLICIASVIG
                     TVLKQHEPLGNYINQFGPFWAEVFARASLYNVYSAWWFLAILGFLVTSTSLCVARNTP
                     KIIADLKSYKENIREQSLKAFGHRAETELKESPEVAARRLGSLLATGGWKVKLQRRET
                     AAGAGWMLAAKAGAANKLGYIAAHSAIVLVCLGGLLDGDLIVRAQMLFNGKTPYNGGG
                     MIADVAKEHRLGDRNPTFRGNLLVAEGTQSDTAILTQSDGILLQELPFAVELKKFVVE
                     YYSTGMPKLFASDIVIHDKETGDKIPAR"
BASE COUNT          720 a         1020 c         1115 g          823 t
ORIGIN      
        1 gtatcttttg ccggacatga attttgaccc ttccgagtcg gtattcgtcc ctttttatgg
       61 ggtcttggcg gcgacgattc catccttgtc ccgctttgca cgcctgtccc gggccaaggt
      121 ggtgccggtc atcacccgca tgaccgcagg agggtatgac gtggaaatac tccccgcatg
      181 gcaagatttt ccaacggccg atgcggcggc agataccgcc ttgatgaacc tgcgcctgca
      241 ggggtatatc gatactatgc cgtcccagta ttactgggtg cacaaacgtt tcaaggatcg
      301 gccttcgggc gaggctgcgc cctattagct agcctttgcg ctgggagaga tagtcctgga
      361 ccagctggat tgccagctct ggctgttcgt ggatgatcca gtgcgtggca tccagaatct
      421 tgcgcaccgt cagatccgga acatacgctt ccagtccctc gatgagcgta ggtggaagcg
      481 ccgtgtcgtt catgccccaa accaccaggg tggggaccac aatggtcagc atggacgccg
      541 gtatctcgac caccgatgcg gctggatcgg ccgccgtagc ggggcgtaac ggagatgcac
      601 ggtagtagtt gcagccaccc tgcaggccgt gtgaccagac attccggtac tgttgtttga
      661 cgtcttcggt gagccagttg tccgccttgc gctggttacc catgtcggta aagaaggccc
      721 acattctcgc gaagtcattg gcggccaggt gctgctctgc acctggttgc accaggaaat
      781 tcatgtaggc gctggcttcc tgctgcgatg ggctgttttt cagttcacgc aggaacgtgc
      841 ccgggtgcgg ggagttgaaa atcaccagct tttccatgcg ctggggcatc tggttggcca
      901 gattccacgc gactgcacca ccccagtcgt gcgcaatcag ggcagcaatt gcgttgtctc
      961 cccctgcctc aatatcaatc aacgcagcaa tatcctgcac caggtgcttg ggacggtagg
     1021 ctgaaacttc ggcgggcgca gtcgattgct caaaaccacg cagattggga gccacgcacc
     1081 ggtagccgcc gttttcaggt cgggagaaat gcgtgagcaa tgcatcccag acgaaggcgc
     1141 cttccggaaa gccgtgcaaa aacaccagaa caggtctgcc acgctggccc gctgcgcgac
     1201 agctcaggct gatcccgtgc ggcagcgctt gttgccaagt ctccatcatg gagcgggagg
     1261 ctgcgacggt tcggggtgtg cccaacgcca cagtagctgt gcgacgtcct cgatgccggt
     1321 gcgcttgggc gcggagaaca gcattacttc gccaccgccg gcctgcagtt tggtaatcga
     1381 cagcgctttg gctccttcgc tgcgggtgag tttgtcggct ttggtcagga ccaccagaaa
     1441 cttcaggcct tccgccacgc gcgggcggat gacatccagc aggatttcat cgagttcggt
     1501 catgccgtgg cgggggtcgc acatcaggac gatgccctgc aggtttttgc ggctgacaag
     1561 gtagttggcc atgacctgtt gccagcggat cttgtcctgt ttaggtacgg ccgcgtagcc
     1621 gtaccccggc aaatccgtga ggacggcatc ggtgatgccc tgttttccca aggcaaacag
     1681 gttgatgtgc tgggttctgc cgggtttttt ggacgcaaag gccaattgct tctgctgcgt
     1741 gagcgtgttg atgcaggtgg atttgccggc gttggagcgc ccgacaaagg caatttcagg
     1801 cacatccagc tccggcagga aatgcagctg gggggctgtt gtcaggaatc ttgccgtgtg
     1861 gagccatccc aatgcaactg tcgcaggcga cttttcgccc ggtgtggacg cagctgcaat
     1921 gggtggcttg gagggtgggt ttgtgctgtt agtcattaaa ttggaagtta aacccgaagc
     1981 gccattgtag aatggcacag ttttgctcac cagacatgac cgccctatga agttgatcgc
     2041 cccactgttt attgccgctg tcgttgcggt gacctccgtc tctgccttcg cagctgaggc
     2101 catacccacg gttgccaagc cagaccttgc caaaggtgag gccagctatg gcgcggtttg
     2161 tgcagcatgc cacggtgtcg atggcaattc cggcacaccg gcctatccca aactgtcgca
     2221 acagcaccct gaatacctgg tcaagcagtt gcaggagttc aaatccgaca agcgcagcaa
     2281 cgcgatcatg aaggggtttg cgtcgatgtt gtcggatgac gacatgaaga acatctctta
     2341 ttggttagcc accaagaagg ccaagcctgg tactgcaaaa gacaaggaat tggtcacgct
     2401 gggcgagcgc atctaccgcg gtggcattgc agaccgccag gtgcctgcct gtgcaggttg
     2461 ccacagccct aatggtgctg gtattcctgc acagtacccc cgtttgagtg ggcagcatgc
     2521 cgactatgca gtggcccagt tgacggcttt ccgtgacggc gttcgtaaaa acagcctgca
     2581 gatgacccag attgcagcca agatgaatga ccgtgaaatc aaggcggttg ccgactacgt
     2641 cgcaggtctt cgttaatctg cgggagttgc cggggttcct cgggaaccaa ttgccggcaa
     2701 cccaccctaa cagggcaggc gcatcgttgg atggcctgct ttttttttga ctgacaccag
     2761 caatccatga cagtttccac ccagggtctt gagttaagta gtggttcgcg tgccgtaaag
     2821 gcagcagtgg agctgctgtc ttccatgcgg tttgccattt cgctgctgac gctgatctgc
     2881 atcgcgtcgg tgatcggcac ggtactcaag cagcacgaac ccctgggtaa ctacatcaac
     2941 cagtttggcc ccttctgggc tgaggtgttc gcgcgcgcca gtctttacaa cgtgtatagc
     3001 gcctggtggt tcctggccat tcttggcttt ttagtaacca gcacttcttt gtgcgtggca
     3061 cgcaacaccc cgaagatcat tgccgatctg aagtcttaca aagagaatat tcgcgagcaa
     3121 agtctcaagg cgtttggtca ccgcgctgaa actgaactga aagagtcacc ggaagtggct
     3181 gcgcgccgtc tgggcagctt gctggctacg ggaggctgga aggtcaagct gcagcggcgc
     3241 gagactgccg ctggcgccgg gtggatgctt gcggccaagg ccggggctgc caacaagctt
     3301 ggctatattg ctgcacacag cgccattgtg ctggtctgtc tgggcgggtt gctggacgga
     3361 gacctgatcg tccgggcgca gatgcttttc aacgggaaaa cgccttacaa cggcggcggc
     3421 atgatcgctg atgtggccaa ggagcatcgt ctcggcgatc gcaaccccac tttccgcggc
     3481 aatttgttgg tcgccgaggg aacgcagtcc gacacggcca ttcttacgca gtccgacggt
     3541 attcttctgc aggagttgcc gtttgccgtg gagttgaaga agtttgtcgt cgagtactac
     3601 tcgacgggca tgccgaaact gtttgccagc gatatcgtga tccatgacaa ggagaccggg
     3661 gacaagattc ctgcacgg
//
LOCUS       DAOSJM010000248         3673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_22146_length_3673_cov_4.759536, whole genome shotgun sequence.
ACCESSION   DAOSJM010000248 DAOSJM010000000
VERSION     DAOSJM010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22146_length_3673_cov_4.759536"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PKX82_09125"
     CDS             complement(<1..591)
                     /locus_tag="PKX82_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOF52456.1"
                     /translation="MAATLAVSGAALADITIGVSLSLTGPGSGLGIPMQNQFKLFPST
                     IAGEKVNLIVLDDASDPGKGAQNARRFTTDDKVDVIIGSCLTPVAAAMADVATGAKTV
                     QLAGSPVGLPPGKDEWVFRLPQSNTVMGHAVVEHMVKQGVKTIGFLGYTDVYGEQWLK
                     ELTPQAEKAGIKIVAVERFARTDTGVTAQALKLVAAN"
     gene            complement(674..1093)
                     /locus_tag="PKX82_09130"
     CDS             complement(674..1093)
                     /locus_tag="PKX82_09130"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOF52457.1"
                     /translation="MSKTVVYEVEVMFGDCDPAGIVFFPNFSKWMDAASLNFFVKCGV
                     PTWRELVKTTGIIGTPLLEINTKFMRPATYGETLQIHTSVQEWRDKVFVHRHVVKRGD
                     DVLCEGTEVRAFCVRLPENPDRIKSIPVPADIKALCS"
     gene            complement(1103..1867)
                     /locus_tag="PKX82_09135"
     CDS             complement(1103..1867)
                     /locus_tag="PKX82_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF52458.1"
                     /translation="MNVQGQAALVTGGGSGLGEATARELARLGAKVAILDVNLAAAQK
                     VASDIGGIAMQCDVTSPDSVQSAITEAQAAHGPARILMQIAGIGSAKRVVGKDGNAAP
                     LEDFAKVINVNLIGTYNVARLFAAACAKLDPMEDGERGAMVFTASVAAFDGQVGQQAY
                     SASKAGVAGMTLPMARDLAQHGIRVCTIAPGLFATPLMRTLPEPVQQSLAASIPFPQR
                     LGKPEEFAQLAVHIVTNGHLNGEVIRLDGALRMAPR"
     gene            complement(1934..3184)
                     /locus_tag="PKX82_09140"
     CDS             complement(1934..3184)
                     /locus_tag="PKX82_09140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOF52459.1"
                     /translation="MASKGFFNAFDDIWMLDGMRTPMVDYCGALGHISPTDLGIKAAK
                     AALAKAGVSPEHINTVLTGNMAPGDYEQFMLPRHIGMYAGVPQSVPALMTQRICGTGF
                     ELFRQAGEQIQSGCADVALVVGSENMTRNPIAAFTHRTGFKLGSPVEFKDFLWEALND
                     SACGINMIQTAENLAKKYGITREEVDVFASASFAKAVAAQESGFLAGEISPVVTEKFE
                     LEGYATRGIKLQGKITEVDKDTHARISPVEVLAKLRAVHAGGVQTGGNSSALVDAAAA
                     AIVASGAYARAEGKKPLARVVAAAVVGVPPEIMGIGPAPAIKLLLERAGLQLDAIGRF
                     EINEAQGAQTLSVGRELGLDLSKLNVNGGAIALGHPLAATGVRLTLTLARELQRSGLR
                     YGVSSACIGGGQGIALLLENPLAS"
     gene            complement(3217..>3673)
                     /locus_tag="PKX82_09145"
     CDS             complement(3217..>3673)
                     /locus_tag="PKX82_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906700.1"
                     /note="catalyzes the conversion of ferulic acid to
                     feruloyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HOF52460.1"
                     /translation="DGRIAEDFKLATGTFVSVGPLRGKIIAAGAPCIQDVVITGLNMH
                     EVGAMVFPNVLGCRNLSGLPADAGMDQVLTSAPVVAHFQKLLNTLAATATGSANRTAK
                     LCLLVEPPSLDRGEVTDKGSINQRAVLACRAAMVDALHEGTLPHTLQPQ"
BASE COUNT          700 a         1161 c         1111 g          701 t
ORIGIN      
        1 gttggcggcc accagcttca gcgcctgtgc agtcacgcct gtgtccgtac gggcgaaacg
       61 ctcaacagcc acaatcttga tacccgcttt ttcagcctgg ggcgtgagct ccttgagcca
      121 ctgctcacca tagacgtcgg tgtagcccag gaaaccaatg gtcttgacac cctgcttgac
      181 catgtgctcc accacggcat ggcccatgac agtgttggac tggggcagac ggaacaccca
      241 ctcgtccttg cccggaggca gacccacggg cgagcccgcc aattgcaccg tcttggcacc
      301 ggttgccacg tcggccatgg ctgcagccac aggcgtcagg cacgagccga tgatgacatc
      361 caccttgtca tcggtggtga agcggcgtgc attctgtgcg cctttgccgg ggtcggacgc
      421 gtcgtccaac acaatcagat tcactttttc gcccgcaatg gtgctgggga acagcttgaa
      481 ctggttctgc atggggatgc ccagacccga gccgggcccg gtcagcgaca gactcacgcc
      541 gatggtgatg tctgccagtg cagcacccga tacggccagt gtggcggcca ttgcgacgag
      601 ggatttaacg agtttcgtgc aaagtctcct ttagtagtag ttggaacggg aacaggaatt
      661 aaatcagggt gagtcaagaa cacaaggcct tgatgtcggc cggcacggga atggatttga
      721 tgcggtcagg attctcgggc aggcgtacgc aaaacgcacg cacttcggtg ccttcgcaca
      781 gcacatcgtc gccgcgtttg accacgtggc ggtgcacaaa taccttgtcg cgccactcct
      841 gcacgctggt gtggatttgc agggtttcgc cataggtggc gggtcgcatg aatttggtgt
      901 tgatctccag cagcggcgta ccgatgatgc cggtggtctt caccagctcg cgccaggtgg
      961 gcacaccaca tttcacgaag aaattcagcg aagctgcatc catccatttg gaaaaattgg
     1021 gaaagaaaac gatgcctgca ggatcgcagt caccaaacat cacttccact tcgtacacaa
     1081 cggttttact catgctcatc tctcagcggg gggccatgcg cagtgcgcca tccagacgga
     1141 tcacctcgcc attgaggtgg ccgttggtca cgatgtggac agcaagctgc gcaaactctt
     1201 cgggcttgcc cagacgctgc ggaaacggaa tgctcgccgc cagagactgc tgcaccggtt
     1261 cgggcaaagt gcgcatcagc ggtgtggcaa agagccccgg agcgatggtg cacacgcgga
     1321 taccgtgctg tgccagatcg cgtgccatgg gcagggtcat gccggccacg cctgccttgg
     1381 acgcactgta ggcctgctga ccgacctggc cgtcaaacgc agccaccgaa gcagtgaaca
     1441 ccatggcacc gcgctcacca tcttccatcg ggtccagctt ggcgcaggca gcagcaaaca
     1501 ggcgcgccac gttgtaggtg ccaatcaggt tgacgttgat caccttggca aagtcttcca
     1561 gtggtgcggc attgccatcc ttgcccacca cgcgcttggc actgccgatg ccggcaatct
     1621 gcatgaggat gcgggccggg ccgtgggcag cttgcgcctc agtgatcgcg ctttgcacgc
     1681 tgtccgggct ggtcacatcg cactgcatgg caatgccacc aatgtcgctg gccaccttct
     1741 gcgcggccgc cagattcacg tcgagtatgg cgaccttggc acccaaccgg gccaattccc
     1801 gtgcggtggc ctcgcccaga ccggaaccac cgccggttac caatgctgct tgtccttgaa
     1861 cattcatcgc tctttccttt tgctattatt ttgatagcta tctacgcaat tgatacgggg
     1921 gctagggccc gatttagctc gcaagcgggt tctccagcaa gagggcaatc ccttgcccgc
     1981 caccgatgca ggcactggac acaccgtagc gcagcccact gcgctgcagc tcgcgcgcca
     2041 gcgtgagcgt caaacgcacg ccagtcgcag ccaaggggtg acccagggcg atcgcgccgc
     2101 cattgacgtt gagcttgctc agatccagac ccagttcgcg gccgaccgac agggtttggg
     2161 cgccctgcgc ctcgttgatc tcaaagcgcc cgatcgcgtc gagttgcaga cccgcgcgct
     2221 ccagcaacaa cttgatggcc ggcgcaggac cgatacccat gatttcgggg ggcacgccca
     2281 ccacagcagc agctaccaca cgggccagcg gcttcttgcc ttcggcacgt gcataggcac
     2341 cggaggccac aatggccgcc gcagcggcat ccaccagcgc cgagctgttg ccaccggtct
     2401 gcacgccacc ggcgtgcact gcacgcagct tggccagcac ttccaccggc gagatgcggg
     2461 cgtgggtgtc cttgtcgact tcggtgatct tgccctgcag cttgatgcca cgggtcgcat
     2521 agccttccag ttcaaacttc tcggtcacca ccggggaaat ttcacccgcc agaaaaccac
     2581 tctcctgcgc cgccaccgcc ttggcgaacg aggcggaggc aaacacatcc acctcttcgc
     2641 gggtgatgcc gtatttcttg gccaggttct ctgccgtctg gatcatgttg atgccgcagg
     2701 cggagtcgtt cagggcctcc cacaggaagt ccttgaactc cacgggtgag cccagcttga
     2761 agccggtgcg gtgggtgaag gccgcaatcg ggttgcgggt catgttctcc gaacccacca
     2821 ccaacgccac gtcggcacaa ccgctctgga tctgctctcc cgcctggcgg aaaagctcga
     2881 agccggtgcc gcagatacgc tgcgtcatca gcgcgggcac cgactgtggc acaccggcat
     2941 acatgccgat gtggcgcggc agcatgaact gctcatagtc gccgggggcc atattgccgg
     3001 tcagcaccgt gttgatgtgc tcgggcgaca cgccggcctt ggccagtgcc gctttggctg
     3061 ccttgatgcc cagatcggtg ggggaaatgt ggcccagagc gccgcagtag tccaccatgg
     3121 gggtacgcat gccgtcgagc atccagatgt catcgaatgc gttgaaaaaa cctttggaag
     3181 ccacgtcaat gtccttctgc ttgcgtgaat cgggtgttat tggggttgga gcgtatgggg
     3241 cagcgtgcct tcgtgcagcg catccaccat ggcagcccgg caggccagta cggcgcgctg
     3301 gttgatggag cccttgtcgg tcacttcgcc ccggtccagc gagggcggct ccaccagcag
     3361 gcacagcttg gcagtgcggt tggcactgcc ggtggccgtc gcagccagcg tgttcagcag
     3421 cttctggaaa tgcgcgacca cgggtgcgga agtcagcacc tggtccatac ccgcatccgc
     3481 cggcagaccg ctcagattgc ggcaacccag cacattgggg aacaccatgg ctccgacttc
     3541 gtgcatgttc aggccggtaa tcaccacatc ctgaatgcag ggagcgccag cggcaatgat
     3601 cttgccgcgc agcgggccga cgctgacaaa ggtgccggtg gcgagcttga agtcctcggc
     3661 aatgcggcca tcg
//
LOCUS       DAOSJM010000249         3655 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_22276_length_3655_cov_4.039167, whole genome shotgun sequence.
ACCESSION   DAOSJM010000249 DAOSJM010000000
VERSION     DAOSJM010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22276_length_3655_cov_4.039167"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..1453)
                     /gene="flhF"
                     /locus_tag="PKX82_09150"
     CDS             complement(8..1453)
                     /gene="flhF"
                     /locus_tag="PKX82_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOF52461.1"
                     /translation="MNIQRFTAATSREALAKARMAFGDSTLILSNRPTEHGVEVVATA
                     EDTLASLGSGTSQFDEQASSGSPKRSSAPSFKDVASKVEEDADQLAMSTLSFQDYVRE
                     RMARKRHEAEQSHAQERAPEPKAVARPVAEVVRRSAPTLDIPVRKPAPVVERKVTATA
                     KPAQAMPQSIVNELHAMKDLIEDRFNTLTWLGQARQNPVQANMMLKMIRAGYSPTLSR
                     AILERMPEDMDPKGALRWVMDILERNLHTDEGATALHDEGGVFALIGATGVGKTTTAA
                     KLAGMCASVHGPGSVGLITLDTYRIGAHEQLRAYGKMLGVVAHLAHDKAALQDLLGLL
                     SNKKMVLIDTTGIAPRDPRKRDLMDLLDLPEIKRLLVLNAGGHGDTLDDAVNCFKTGN
                     AQHTLLSKTDEAVKLGPAIDASIRHQLILRGITTGQRVPEDWEPAVASNLVRMSMRSA
                     NTSAFDPNMSDLGFFFSQPSDRAMGRGHLNA"
     gene            complement(1450..3528)
                     /gene="flhA"
                     /locus_tag="PKX82_09155"
     CDS             complement(1450..3528)
                     /gene="flhA"
                     /locus_tag="PKX82_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOF52462.1"
                     /translation="MNPQLKNLQQWFGTNSAHVRGAAAPMLVMAILAMMVLPLPPLVL
                     DVFFTINIAVALMVMMVAAYMIRPLDFAAFPSVLLLTTLMRLSLNVASTRVVLLEGHT
                     GPGAAGAVIEAFGHFLIGGNFAVGLIVFAILVVINFVVVTKGSERIAEVSARFTLDAM
                     PGKQMAVDADLNAGLIDEKEAKRRRAEIGEEAEFFGSMDGASKFVRGDAIAGILILLI
                     NIFGGFAIGILQHDLTASQAANTYILLAVGDALVAQIPGLLISVAAAMVVSRVGKDKD
                     IGKQIVGQMFVSPKVLGITAVIMFILGVIPNMPHFVFLSISAVLGYGAWMLAHQPVVE
                     EAPAVAPAAAHDGEASWDDLQPVDQLGLELGYRLIALVDKARQGDLLNRIKGVRRKFA
                     QEVGFLPPPVHVRDNLELKPSAYRITLRGVIVGEGEAFPGMYLAINPGGITTPLIGTP
                     TTDPAFGLPAHWIDEKQKEAAQLAGFTVVDSETVMATHLSHLMQVQASKLLSRTETQQ
                     LVEHVAKLAPKLIEEVVPKMVSVAVFQKVLQLLLDEAVHIRDIRTIIESIAEHATTTT
                     DPAELARRVRVALSPAIVQQIYGPTRELNVIAIDPSLERLLVQALGNASGPALDPGVA
                     DILTRTAAETALKQEEIGIPACLLVPDQIRGSIARLVRRVAPRLQVLAHSEIPETHSI
                     RIGPILRGAA"
     gene            complement(3525..>3655)
                     /locus_tag="PKX82_09160"
     CDS             complement(3525..>3655)
                     /locus_tag="PKX82_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar biosynthetic protein FlhB"
                     /protein_id="HOF52463.1"
                     /translation="LAYVYRLRAAMRGDGPMPGDVPQPAVPPELDPFTKTAKAATP"
BASE COUNT          663 a         1061 c         1208 g          723 t
ORIGIN      
        1 acgtcgatca tgcattcaga tgcccccgtc ccatggcacg gtcggagggt tggctgaaga
       61 aaaatccaag gtcgctcata ttcgggtcga aggcagaggt gttggccgag cgcatggaca
      121 tgcgcaccag gttggacgca accgcgggtt cccaatcttc cggcacccgc tgcccggtgg
      181 tgattccgcg cagaatcaac tggtggcgga tgctggcatc gatggccggc cccaatttga
      241 ccgcttcgtc ggttttggag agcagggtgt gctgtgcgtt gccagtcttg aagcagttga
      301 cggcgtcatc cagggtgtcg ccatgtccgc cagcgttcag taccagcagg cgcttgattt
      361 cgggcaggtc cagcaggtcc atcagatcac gcttgcgcgg atcccgcggg gcaatgccgg
      421 tggtatcaat caataccatt ttcttgttgg acaacagacc cagcaggtcc tgcaacgcgg
      481 ccttgtcgtg ggccagatgg gctaccacgc ccagcatctt gccgtaggcg cggagttgtt
      541 cgtgcgcacc gatgcggtag gtgtcgagcg tgatcaggcc cacgctgcct gggccgtgca
      601 cgctggcaca catgcctgcc agcttggccg cagtcgtggt cttgcccaca ccggtagcac
      661 cgatcagggc aaacacaccc ccttcgtcgt gcaatgcggt ggcaccttcg tcggtgtgca
      721 ggttgcgttc cagaatatcc atgacccagc gcaacgcacc tttggggtcc atgtcttcgg
      781 gcatgcgctc caggatggcg cgggacagcg tgggcgagta acccgcgcgg atcatcttga
      841 gcatcatgtt ggcctgcacc gggttctggc gtgcctgtcc gagccacgtc agggtgttga
      901 agcggtcttc aatcaggtcc ttcatggcgt gcaattcgtt gacgatgctc tggggcatgg
      961 cctgcgcagg cttggcggtt gcggtcacct tgcgctccac cacgggtgcg ggtttgcgca
     1021 ccgggatgtc cagggtgggg gcgcttctgc gcaccacttc ggcaacgggt cgggcaacgg
     1081 ccttgggttc gggcgcgcgc tcctgcgcat ggctctgctc tgcctcgtgg cgtttgcgcg
     1141 ccatacgttc gcgcacatag tcctgaaacg acagggtgct catggccagc tggtcggcgt
     1201 cttcctccac cttgctggcg acatccttga agctcggagc actggagcgc ttgggcgagc
     1261 cagaagaggc ctgctcgtcg aactgactgg tgccactgcc caggctggcc agcgtgtctt
     1321 cagccgtggc cacgacttca acgccgtgtt ctgtcgggcg gttggaaaga atcagcgtgc
     1381 tgtctccaaa ggccatgcgg gctttggcga gtgcttcgcg ggatgttgct gcggtaaagc
     1441 gctggatgtt catgctgcac ctctaagaat ggggccaatg cggatggaat gggtttcggg
     1501 tatttcgctg tgtgccagca cctgcaggcg cggggcgacg cggcgtacca gccgggcgat
     1561 cgagccgcgg atctggtcag gcaccagcag gcaggcagga atgccaattt cttcctgctt
     1621 gagtgcggtc tcggcagcgg tccgggtcag gatgtcggcc acgccggggt ccagtgccgg
     1681 tccggatgcg ttgccgagag cctgcaccag caggcgttcc aggctggggt caatggcaat
     1741 cacattgagt tcacgggtcg ggccgtagat ctgctgcacg atggctggcg acagtgccac
     1801 ccggacccgg cgtgcgagtt ctgcgggatc ggtggtggtg gtggcgtgct cggcaatgga
     1861 ttcgatgatg gtgcggatgt cgcggatgtg gaccgcctcg tccagcagca gctggagcac
     1921 tttctggaag accgcgacgg aaaccatctt gggaaccact tcctctatga gtttgggggc
     1981 cagtttggcg acgtgttcca cgagttgttg ggtctctgta cggctgagca atttggaggc
     2041 ttgaacttgc atcaagtgtg acaaatgggt cgccatgacg gtctcggaat caaccaccgt
     2101 aaatccggcc aattgcgcgg cttccttctg tttttcgtcg atccagtgcg cgggcagacc
     2161 aaaggccggg tcggtggtgg gggtgccgat cagtggcgta gtgatgccac ccgggttgat
     2221 ggccaggtac atgccgggga atgcttcgcc ttcccctacg atgacgccac gcagggtgat
     2281 gcggtaggcg ctgggtttga gttccaggtt gtcacgcacg tggacaggcg gtggcaggaa
     2341 acccacttcc tgagcgaatt tgcggcgcac acccttgatg cggttgagca gatcaccctg
     2401 gcgggccttg tcgaccagcg cgatcaggcg gtagcccagc tccagaccca gttgatcgac
     2461 cggctgcaag tcatcccagg aggcctcgcc atcgtgtgca gcggcagggg ccacggcggg
     2521 agcttcttcc accaccggct ggtgtgccag catccaggct ccgtagccca gcacggccga
     2581 gatggacagg aagacaaagt ggggcatgtt gggaatcaca cccaaaatga acatgatgac
     2641 cgccgtgatg cccagcactt tgggcgatac gaacatctgg ccaacgatct gcttgccaat
     2701 gtccttgtcc ttgccgacac gggacaccac catcgctgcc gccaccgaga tcagcaggcc
     2761 gggaatctgg gccaccagcg cgtcaccgac ggccaggaga atgtaggtgt tggcggcctg
     2821 cgatgcggtc aggtcgtgct gcaggatgcc aatggcaaaa ccgccaaaaa tattgattag
     2881 caggatcagg atgccggcaa tcgcatcgcc ccgcacaaat ttggacgcgc cgtccatgga
     2941 gccaaagaat tcggcttctt cgccaatttc tgcccggcga cgtttggctt ccttctcgtc
     3001 gatcagaccg gcgttgaggt cggcgtccac cgccatctgt ttgcctggca tggcgtccag
     3061 ggtgaagcgg gcagacactt ccgcgatacg ttcggagccc ttggtgacca ccacaaagtt
     3121 gatcaccacc agaatcgcaa acacgatcag acccacggca aagttgccgc caatcaggaa
     3181 gtgaccaaag gcttcgatca ccgcacccgc tgcgcccgga ccggtatggc cttcgagcaa
     3241 tacgacgcgg gtggatgcga cgttgaggga caggcgcatc agcgtggtga gcagcagcac
     3301 cgacgggaat gcggcgaagt ccagcgggcg aatcatgtag gcggccacca tcatcaccat
     3361 cagggcgact gcaatattga tggtgaagaa gacgtccaga accagcgggg gcagcggcag
     3421 caccatcatg gccaggatgg ccatgaccag catgggggcc gctgcaccac gcacgtgggc
     3481 ggaattggtt ccgaaccact gctgcaggtt tttcagttgc gggttcatgg agtggctgct
     3541 ttcgcggttt tggtgaaggg atcaagttct ggcgggactg cgggctgcgg cacgtcgccc
     3601 ggcatggggc cgtcaccccg catggccgcg cgcaggcggt agacataggc cagca
//
LOCUS       DAOSJM010000250         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_22422_length_3636_cov_3.600670, whole genome shotgun sequence.
ACCESSION   DAOSJM010000250 DAOSJM010000000
VERSION     DAOSJM010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22422_length_3636_cov_3.600670"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PKX82_09165"
     CDS             complement(<1..961)
                     /locus_tag="PKX82_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HOF52464.1"
                     /translation="MIKKILIALLALVVVLAAAVGINTARKGSRQMQVEPLAPVAVDE
                     AAVAARLAEAVRLKTISSRDDANLNADQFKAFHALLQTRFPKTHAALKREVVGDLSLL
                     YTWEGADPQAKPILLMAHQDVVPVAPGTEGDWAVAPFAGEVKDGFVWGRGSWDDKGNL
                     LSQLEAVEMLVASGYKPPRTIYLAFGADEEVGGLRGAAKVSALLKSRGVQLDFVLDEG
                     LLVLEGVMPGIAKPTALIGVAEKGYMSVVLKMSATPGHSSMPPKKGTSAIAMMSAALN
                     RLEDEQLPGGIRGVASEMFDTLAPEMGGFSRVAVSSLWLFGPVV"
     gene            complement(1092..1391)
                     /locus_tag="PKX82_09170"
     CDS             complement(1092..1391)
                     /locus_tag="PKX82_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52465.1"
                     /translation="MHLDYLSFESSDDGEGTGSWDAMASVRPAMLAQVLAETQTVLAW
                     AEANSPGPRGPLDDGGVWDADQHTQTEGDWTTVTLTLTGPCAWGEAMLTHFGQDA"
     gene            1524..1865
                     /locus_tag="PKX82_09175"
                     /pseudo
     CDS             1524..1865
                     /locus_tag="PKX82_09175"
                     /inference="COORDINATES: protein motif:HMM:NF023338.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3429 domain-containing protein"
     gene            1935..2591
                     /locus_tag="PKX82_09180"
     CDS             1935..2591
                     /locus_tag="PKX82_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOF52466.1"
                     /translation="MQLNTDLSQRCVVDSNTLPWSESPSPLVQRRLLERNGGEVARAT
                     SVVRYAAGARFDAHTHALGEEILVLEGTLSDALGDYGPGTYLKNPPGSAHAPFSERGC
                     TLFVKLRHLDPQDPERVVVSTRQAPWRQGLVPGLTVLPLWEFGTTHTAMVRWAPGTYF
                     NPHRHYGGEEIFVVEGVFSDEHGNYPQGSWIRSPHLSQHQPFSREGCLILVKTGHLLQ
                     "
     gene            complement(2713..>3636)
                     /locus_tag="PKX82_09185"
     CDS             complement(2713..>3636)
                     /locus_tag="PKX82_09185"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HOF52467.1"
                     /translation="LPDSTVKLGIMDEERRTSVNLKACIAAAIDRVAFINTGFLDRTG
                     DEMHTAMLAGPMMRKGDMKSSAWIAAYERNNVLVGLQCGLRGKAQIGKGMWAMPDLMA
                     EMMKQKIGHPKAGANTAWVPSPTGATLHALHYHQVLVSDIQKDLEKTKYAKVRNELLN
                     GLLTVPVTATPNWTDAEKQAELDNNAQGILGYVVRWVDQGVGCSKVPDINDIGLMEDR
                     ATLRISSQHMANWLHHGVVTKAQVEETFKRMAAKVDKQNAGDKAYTPMANNEKGAAYQ
                     AACDLVFKGLEQPSGYTEPLLHAWRLKVKAA"
BASE COUNT          593 a         1135 c         1169 g          739 t
ORIGIN      
        1 gcaccacggg gccaaacagc cacaggctcg agaccgccac acgcgagaag ccacccatct
       61 cgggggccag cgtgtcaaac atttcactgg ccacaccgcg gatgccgccg ggcagctgct
      121 cgtcttccag gcggttcagt gctgcgctca tcatggcaat ggcgctggtg ccctttttgg
      181 gtggcatgga cgagtggcca ggcgtggccg acatcttgag cacgaccgac atgtagccct
      241 tctcggccac accaatcagc gcggtgggtt tggcaatgcc aggcatcacg ccctccagca
      301 ccagcaggcc ttcgtccagc acaaagtcca gttgcacgcc acggctcttg agcagggcgg
      361 acaccttggc ggcgccacgc aggccaccga cttcctcgtc tgcgccgaag gccaggtaga
      421 tggtgcgggg gggcttgtag ccgctggcca ccagcatctc caccgcttcg agttgcgaca
      481 gcaggttgcc cttgtcgtcc cacgagccgc ggccccagac aaagccgtct ttcacttcac
      541 cggcaaaagg ggccaccgcc cagtcgccct cggtgccggg cgcgacgggc accacgtcct
      601 ggtgggccat cagcagaatg ggtttggctt gggggtcagc gccctcccag gtgtagagca
      661 ggctcaaatc acccaccacc tcgcgcttga gtgccgcgtg ggtcttgggg aaccgggttt
      721 gcagcagggc gtgaaaagcc ttgaactggt cggcgttgag gttggcgtcg tcacgcgacg
      781 aaatggtctt gaggcgcact gcctcggcca ggcgggccgc tacggcggct tcgtccaccg
      841 ctaccggcgc cagcggttcc acctgcatct ggcgcgagcc tttgcgcgcg gtgttgatgc
      901 cgaccgctgc ggccagcacc actaccaggg ccagcagggc tatgagtatt ttcttgatca
      961 ccagatgtct cctcgtgttg tggcgagcat cttacgatac gtgcggaggg tgcattctgc
     1021 ggtggatttg aaggcaaatt ggcctctagc ccccgcggaa tatgcgcgag tagctcctaa
     1081 atcaggagcg tctatgcgtc ctgcccaaaa tgcgtcagca tggcctcgcc ccatgcgcag
     1141 ggtccggtga gtgtcagcgt gaccgtggtc cagtcgcctt cggtctgggt gtgctggtcg
     1201 gcatcccaca cgcccccgtc gtccagcgga ccgcggggtc cggggctgtt ggcttctgcc
     1261 caggccagca ccgtctgggt ttcggccagt acctgcgcca gcatcgccgg gcgcacgctg
     1321 gccatggcgt cccagctgcc ggtgccttcg ccgtcgtcgc tggattcaaa actcaggtag
     1381 tccaggtgca tgcgctggat tatctgcaga tcccagtctg gcccgctgcg ctgaaagtcc
     1441 ccaagaccat tcaacctatg ccgaaacggt gaaaattgaa cgcacccaat ttggaggagt
     1501 tatcccatgc aagaagacgt cgtatgagtc atgtgaatcg aagtacgccc agccgtggcg
     1561 cacaggcatt gggctacgga ggattgatac cgtttgtggg gctggcgctg gcggtatggt
     1621 ccatgcctgc gcatcaggct gttgcggcgt ccgcgctgct ggcctactgc gccaccatcg
     1681 tgagttttct gggtgccatc cactggggct tgaccatgcg tgatgccact ggccaaagca
     1741 cccgtatgct ggtgtggggt gtgtttccga gcttgctcgc atgggttgcg ctactgctac
     1801 ccgttgccac cgggatggtg cttttggcgg taattttgtg ggcctgtttt gcggttgacc
     1861 gcaggaccta cccccgccag cttgagctgt atcgccaccg cagtgcgcct gaccggagca
     1921 gcctggtgaa cgacctgcaa ctgaacaccg acctgtcaca gcgctgcgtg gtggattcca
     1981 acacgctgcc ctggagcgaa tcaccatcac cgctggtgca acgcaggctg ctggagcgca
     2041 acgggggaga agtggcgcgc gcaacctcgg tggtgcgtta cgccgcgggt gcccggtttg
     2101 atgcacatac gcatgcgctc ggcgaagaaa tactggtact ggaaggtacc ttgagtgatg
     2161 cgctgggcga ctacggccct ggcacgtacc tcaaaaaccc acccggctcc gcgcacgcac
     2221 ctttctctga gcgtggctgc accttgtttg tcaagctgcg acatttggac ccacaagacc
     2281 cagagcgcgt ggtggtttct acacgccaag ccccatggcg ccagggcctc gtgccaggcc
     2341 tgaccgtgtt gcctctgtgg gagtttggca ccacacatac cgccatggtg cgctgggcgc
     2401 caggcactta cttcaacccg caccggcatt acggtgggga ggagattttt gtggtggagg
     2461 gcgttttttc agatgaacat ggcaactacc cgcaaggtag ctggatacgc agtccccacc
     2521 tcagccagca ccagcccttc agccgcgagg gttgcctgat tctggtcaaa acagggcatt
     2581 tgctgcagta gcgcggcagg tcagccgtgt gagtgcccat gcaaaggggc tacaagccaa
     2641 attggcctgt agcccccgta ttcattgcgc aagcagctac tgaaacagta gcgcctgcgc
     2701 ccggctgtcg gcttacgccg ctttgacctt cagacgccag gcgtgcagca gcggctcggt
     2761 gtagccgctg ggttgctcca ggcccttgaa caccaaatcg caggcagcct ggtaggcggc
     2821 gcccttttcg ttgttggcca tgggggtgta ggccttgtcg ccagcgtttt gcttgtccac
     2881 cttggcggcc atgcgcttga aggtctcttc gacctgggcc ttggtcacca caccgtggtg
     2941 cagccagttg gccatgtgct ggctggagat gcgcagcgtg gcgcggtctt ccatcaggcc
     3001 gatgtcgttg atgtcgggca ccttggagca gcccacgccc tggtcgaccc agcgcaccac
     3061 gtagcccagg atgccctggg cgttgttgtc gagctcggcc tgcttttcgg cgtcggtcca
     3121 gttgggtgtg gccgtcacgg gcacggtcag cagaccgttg agcagttcgt tgcgcacctt
     3181 ggcgtacttg gtcttttcca ggtccttctg gatgtcggac accagcacct ggtggtagtg
     3241 cagggcgtgc agggtggcgc cggtggggct gggcacccag gcggtgttgg caccggcctt
     3301 ggggtggcca atcttctgct tcatcatctc ggccatcagg tcgggcatgg cccacatgcc
     3361 cttgccgatc tgcgccttgc cgcgcaggcc gcactgcagg cccaccagca cgttgttgcg
     3421 ctcgtaggcg gcgatccagg cgctgctctt catgtcgccc ttgcgcatca tcgggccggc
     3481 cagcatggcg gtgtgcatct cgtcgccggt gcggtcgagg aagccggtgt tgatgaaggc
     3541 cacgcggtcg atcgcagcgg caatgcaggc cttcaggttg acgctggtgc ggcgctcttc
     3601 gtccatgatg ccgagcttga cggtgctgtc gggcag
//
LOCUS       DAOSJM010000251         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_22434_length_3634_cov_5.080749, whole genome shotgun sequence.
ACCESSION   DAOSJM010000251 DAOSJM010000000
VERSION     DAOSJM010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22434_length_3634_cov_5.080749"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..3302
                     /locus_tag="PKX82_09190"
     CDS             180..3302
                     /locus_tag="PKX82_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF52468.1"
                     /translation="MPLLQRLLPNSLVGRVFTLYLASLVLFVGVGLGLFYRYQFTQQI
                     EDELLAGEMMMNVAAQTVGDSAVIGDYDTITKTLERAIARSNFAQAQFIDTTGGVITA
                     SNTVRHALVPPDWLRAQVREQLYDINHNIVVGGKDYGVLRLKFADEAIAGKLWQVALF
                     AMALSLAALVLGVVGIRIPLKRWLGNFDRVRARETEILSGALNINALIDSDAPEEIRH
                     TFDILHRAAERLLAQREEASVTLNAITDGVLTTDASYRLVYCNPAAEQMLGVHGQNII
                     GQDAKSLLPMAFADEDNPADWKVRKLEVLTPGGGRAILDTTLSTIYSASHEVAGHVLA
                     FRDVTQQHQLDQQLRNELQMRQRALESLRGVLDTFQTPVDAASGPLAADDLDALSVRV
                     VNLINERELSRRALDNQKFALDQHAIVSMTDLQGNITYANERFCDISGYSRAELLGTN
                     HRIVHSGTHPPEVFAELWESISHGRVWHGEICNRKKNGSLYWVDATMVPLLGVDGLPE
                     QYIAIRTDITVRKTFEAQLAEQLRFVEVLLEATPTAIYLKDLQGHYLRFNKAFADLFG
                     IERGAWIGKTVFDLVPGEAAQMIHAKDLELFRTGTVQTYEAGFTNLTTGEVREGLYWK
                     APLTDAQGKNTALVGTVLDITEKNRFEQALRDAKRNAEAANQAKSDFLANMSHEIRTP
                     MNGVIGMTDLALGTSLNAIQREYLSIVKSSAQSLMVILNDILDFSKIEAGKLNIEAVE
                     FPLDEWIAETLKTLVARADKKGLVLHRHLAPDLPGRVLGDPVRIRQVLTNLCDNAIKF
                     TAQGGVYVDVRCTAGDGGYSELQFSVRDTGIGIPADKQQGIFEAFTQADASTTRQFGG
                     TGLGLTICARLVELMDGRIWVESVPGHGSTFHFTVGVQSVSAPAPAVVATVAEPTGAQ
                     GPSLQVLLVEDHPINQMLATTLLKKWGHAVVLAKNGQEGVNLFPGQAWDLVLMDMQMP
                     VMGGLEATRLIRASERPGQRTPIVAMTANAMESDRQACLEAGMDDHLAKPFNARDLLA
                     VMARYTTRQS"
     gene            complement(3373..>3634)
                     /locus_tag="PKX82_09195"
     CDS             complement(3373..>3634)
                     /locus_tag="PKX82_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52469.1"
                     /translation="HGRLLGGPEDTVPMLLFFSVARSASTSQAERATEATSETASIES
                     TAGVDLVTETGEKMPTDKLLDVSPLALRMSSPFNAGYWLAAA"
BASE COUNT          738 a         1059 c         1132 g          705 t
ORIGIN      
        1 gccgctgcct ttgtggcgat ggccaaagat cccaagggac aggaaatcct gcaccaggcg
       61 tcccgggaag tgggcttggc tgcggacgcc tacttcattc ctgcatccgg tgcggattac
      121 gccgcttacc gccgcttcta ccagtcggcg cccgcctccc tgcattaaga ccgactgcga
      181 tgcccttgct gcagcgcctg ctacccaatt cccttgtggg aagggtgttc acgctgtatc
      241 tcgcttcgct ggtgcttttt gtgggtgtcg gactggggct cttttaccgt taccagttca
      301 cccagcagat cgaagacgaa ttgctggcgg gcgaaatgat gatgaatgtg gccgcccaga
      361 ccgtgggcga cagcgccgtc attggggatt acgacaccat taccaagacg ctggagcgtg
      421 cgatagcccg ttccaacttt gcacaggcgc agtttattga caccactggc ggtgtgatta
      481 ccgcttccaa tacggtccgg cacgcactgg ttccgccaga ttggctgcgt gcccaggtgc
      541 gtgagcagct ctatgacatc aaccacaaca tcgtggtggg tggcaaggat tacggcgtgt
      601 tgcggctgaa gtttgccgac gaggcgattg ccggcaagct ctggcaggtg gcgctgttcg
      661 ccatggcact gtcactggct gccctggtgc tcggggttgt cggcatccgc attcccctca
      721 aacgctggct tggcaatttc gatcgggtgc gggcccggga gaccgaaatc ctgtctggcg
      781 cactgaacat caatgccctg attgacagcg atgccccgga agaaatccgc cacacttttg
      841 acattcttca ccgtgcggca gaacgtctgc tcgcgcagcg cgaggaggca tcggtcacgc
      901 tgaacgcgat caccgacggc gtactgacaa ccgacgcttc gtatcggctg gtgtactgca
      961 atccggctgc ggaacagatg ctcggcgtgc atggacagaa catcatcggt caggatgcca
     1021 agagcctgct gccgatggcc ttcgccgacg aggacaaccc ggcggactgg aaggtacgca
     1081 agctcgaagt actgacgccc ggcggcgggc gcgccattct ggataccacg ctgtcgacca
     1141 tttactccgc cagccacgag gtggcggggc atgtgcttgc gttcagggat gtcacacagc
     1201 agcaccagct ggaccagcag ctgcgcaacg agttgcagat gcgccagcgg gcattggagt
     1261 ccctgcgcgg ggttctggat acatttcaga caccggtaga tgctgcatcc ggtccgctgg
     1321 cggcggacga tctcgatgcc ttgagcgtgc gggtggtcaa cctgatcaat gaacgcgagc
     1381 tcagtcgccg ggcactggac aaccagaagt ttgcactgga ccagcatgcc attgtcagca
     1441 tgacggacct gcagggcaac atcacctatg ccaacgaacg cttctgcgat atcagcggct
     1501 attcgcgtgc ggaattgctg gggaccaatc accgcattgt ccattcaggg acccatccac
     1561 ccgaggtatt tgcggagttg tgggaatcga tctcccacgg ccgggtctgg catggcgaga
     1621 tttgcaatcg caagaaaaac ggatcgctgt actgggtcga tgccaccatg gtcccgttgt
     1681 tgggagtcga tggcttgccg gagcagtaca tcgcaatccg aacagacatc accgtgcgca
     1741 agacgttcga ggcccagctg gccgagcaac tccggtttgt ggaagtcctg ctggaagcga
     1801 ctccaaccgc gatctacctc aaggacctgc aggggcacta cttgcgtttc aacaaggcct
     1861 ttgcagacct cttcgggatt gagcggggag cctggattgg caagaccgtg tttgacctgg
     1921 tgccaggcga ggcggcacag atgatccacg ccaaggatct ggaattgttc cgtaccggca
     1981 cggtacaaac gtatgaggcg gggtttacca acctcaccac cggggaggtc cgagagggcc
     2041 tgtactggaa ggcgccactg accgatgcgc aagggaaaaa caccgccctg gtggggacgg
     2101 ttctcgatat taccgagaag aaccggtttg agcaggccct gcgtgatgcc aagcgcaacg
     2161 ccgaagcagc caaccaggcc aaaagcgatt tcctggccaa tatgagccac gaaatcagga
     2221 cgccgatgaa cggagtcatc ggcatgaccg atcttgcctt gggtacatcg ctgaacgcca
     2281 tccagcgcga gtacctgtcg atcgtgaaaa gctcggccca gtccctgatg gtcatcctca
     2341 acgatatcct ggacttttcc aagattgagg caggcaagct caacatcgag gcggtggagt
     2401 ttccgttgga tgagtggatt gccgagaccc tcaaaaccct ggtcgcccgt gcggacaaaa
     2461 aggggctggt attgcatcgt catctggcgc cagatttgcc cggccgggta ctgggcgatc
     2521 ccgtgcgcat acgccaggtg ctcaccaacc tgtgcgacaa cgcgatcaaa ttcacggcac
     2581 aaggcggtgt gtatgtcgat gtgcgctgca ccgccggaga tggcgggtac agtgagctgc
     2641 agttttcggt gcgtgatacc ggcatcggca tcccggcaga caagcagcaa ggtatttttg
     2701 aagccttcac ccaggcggat gcatccacca cccggcagtt cggcggcacc ggactgggcc
     2761 tgaccatttg tgcccgtctg gtggagctga tggatgggcg tatctgggtg gaaagcgtcc
     2821 cgggccacgg cagtacgttc cactttacgg tgggtgtcca gagcgtgagt gcacctgctc
     2881 ccgctgtggt ggccacggtg gccgagccca cgggcgcgca ggggccatcg ctgcaggtgc
     2941 tcctggttga ggaccatccc atcaaccaga tgctggccac aaccctgctc aaaaaatggg
     3001 gccatgcggt ggtccttgca aagaatggtc aggaaggggt gaacctcttt ccgggccagg
     3061 cctgggatct ggtcctgatg gacatgcaga tgccggtgat gggcgggctg gaagcaacgc
     3121 gcctgattcg ggccagtgaa cgtccaggtc agcgcacgcc tatcgtcgcc atgacggcca
     3181 acgccatgga gtcggaccgc caggcttgcc tggaagccgg tatggacgac catctggcca
     3241 agccgttcaa cgccagggac ctgctggccg tcatggcccg ctacacgacg cggcaaagct
     3301 agcgggcaat ccgccaagaa ccaaaaaaaa gcccgtcacg catgacgggc ttttggagca
     3361 aggcgggtag cctcacgcgg ctgcgagcca ataccctgcg ttaaaggggc tgctcatgcg
     3421 caacgccagg ggtgatacgt ccaacaactt gtcggtaggc atcttctcgc cggtctcggt
     3481 gaccagatcg acgcccgcag tgctttcgat ggatgcggtt tcgctggtag cctcggtcgc
     3541 ccgttctgct tggctggtgg atgcagaacg ggctacagaa aagaatagaa gcatcggaac
     3601 ggtatcttcc ggtcccccca gtagtcggcc gtgt
//
LOCUS       DAOSJM010000252         3616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_22569_length_3616_cov_3.565291, whole genome shotgun sequence.
ACCESSION   DAOSJM010000252 DAOSJM010000000
VERSION     DAOSJM010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22569_length_3616_cov_3.565291"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PKX82_09200"
     CDS             <1..463
                     /locus_tag="PKX82_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOF52470.1"
                     /translation="GDTAFRAQRDALEHAQLALKKLAAQAHGEALTQLMTAWEKRDAS
                     LVPTVQELGSRTTPVVRTSWLQAVSESKVAAKSVTPQEALLRLEMAAEVPTPAELLSD
                     RRALQLQLLTRRNDPTPAQTWGQDTAVVLAGAHGTQEARRLQNALKVLLKK"
     gene            510..2150
                     /locus_tag="PKX82_09205"
     CDS             510..2150
                     /locus_tag="PKX82_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOF52471.1"
                     /translation="MDSVDDLLRRLEQLNAIGAALSNERNITQLLETILVAAKNITHA
                     DGGTLYRVSDDGQALRFEILRTTSLGISLGGSSGQHVPFPDLPLRNAQGQPNDSLVAA
                     YSAIHNQTVNIADAYTEVNFDFSGTRQFDARTGYRSRSFLSVPMDDHDGQVIGVLQLI
                     NACKPGTDEVVPFSAADQSLVESLASQAAIAITSRNLFTQMEELFESFISVINLAIDE
                     KSHYTGGHCERVPSLTMMLADAVNATQTGPLADFVMNDRDRYELKIAGLLHDCGKVTT
                     PVHVVDKATKLQTLFDRIDLLDTRFEVLKRDQEIATLRAQLALRPVQDVAGETNLQAQ
                     CRQKITALEEDRDFLRQVNLGAESMPEADQQRVRHIGATNVWRNPDGVQTEFLTADEV
                     ENLTVRSGTLTASERETINYHIVATIKMLQQMPWPRHLRHVPEYAGGHHERMDGKGYP
                     KGLTREQMSVQARIMGIADIFEALTAKDRPYKQGMTLSQAMAIMHKFSRNGHIDPDLF
                     DVFVKTGVYRRYAELFLDPWQIDEVNPAAWLPKPAQSA"
     gene            complement(2107..2892)
                     /locus_tag="PKX82_09210"
     CDS             complement(2107..2892)
                     /locus_tag="PKX82_09210"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOF52472.1"
                     /translation="MHIDAPPQWGCVDFISDLHLQATDTATLDAWSQYLHGTTADAVF
                     ILGDLFEVWVGDDAIADANSFEARCTQVIADAAARRPVYIMHGNRDFLMGQQLMAACG
                     AQLLSDPCVLAFAGERWVLTHGDALCLDDLPYQAFRTSVRSAAWQQAFLAKPLAERQQ
                     IARALRDQSEARKAVETEYADIDTPAAIALLREANAHHMIHGHTHKPGHHTLGPNLER
                     TVLSDWDLLARTPRAEVLRIAASTTARERIRRIAPALATTRQD"
     gene            complement(2910..3416)
                     /locus_tag="PKX82_09215"
     CDS             complement(2910..3416)
                     /locus_tag="PKX82_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF52473.1"
                     /translation="MSKPQVELHVANYGVITLELDAEKAPKSVANFLAYVNKGHYNNT
                     IFHRVIPGFMVQGGGMEPGMAQKTCDAPIENEANNGLKNANYTVAMARTGDPHSATAQ
                     FFINVADNGFLNHTSPSQQGWGYAVFGKVISGTDVVDKIKAVKTGRKGFHDDVPKEDV
                     VIEKAVAL"
     gene            complement(3420..>3616)
                     /locus_tag="PKX82_09220"
     CDS             complement(3420..>3616)
                     /locus_tag="PKX82_09220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOF52474.1"
                     /translation="FVNVVDNSGLNAPQPDGNGYAVFGKVVQGMDTVDKIRGVATTNK
                     GMYQNVPATPIVINSATLVK"
BASE COUNT          726 a         1008 c         1128 g          754 t
ORIGIN      
        1 gggtgacacg gccttccgcg cccagcgtga tgcgctggaa catgcccagc tggcgctgaa
       61 gaagctggcg gcacaagccc acggcgaagc actgacgcaa ctgatgactg cctgggaaaa
      121 gcgcgatgcc agcctggtcc cgaccgttca ggaactgggc agcaggacca cgccagttgt
      181 gcggacatcc tggttgcagg cggtgtccga gtccaaggtg gctgcaaaaa gcgtcacccc
      241 acaggaagcg cttttgcgac tggaaatggc agcagaagtg ccgactccgg cagaattgct
      301 atcggatcgc cgggctttgc agcttcagtt gctgacccgc cgtaacgacc cgactccggc
      361 gcagacctgg ggacaggaca cggccgtggt attggctggt gcccatggta cgcaagaggc
      421 acgccggttg cagaatgctc tgaaagtgtt gctgaaaaag taagttggct accgtggcac
      481 ggtgccatgg tggatacagg agaggtgcct tggactctgt ggatgacctt ctgcggcggc
      541 tggagcaact caacgccatt ggcgcggcgc tttccaatga gcgcaacatc acgcagcttc
      601 tggaaaccat tctggtggct gcgaagaaca tcacccatgc cgatggcggc accctgtacc
      661 gcgtgtcaga cgacggtcag gccctgcgct ttgagattct gagaaccact tcgttgggta
      721 tttccctggg gggcagttcc gggcagcacg tgcctttccc tgacctgccg ttgcgtaacg
      781 cgcaggggca gcccaacgat tcgctggtgg ctgcctattc ggcgatccac aaccagacag
      841 tcaacattgc cgatgcctac accgaggtca attttgactt ttccgggaca cgtcagttcg
      901 atgcgcgtac gggataccgc tcaaggtctt tcctgtccgt gcctatggat gaccacgatg
      961 gccaggtgat tggcgtgctc cagctgatca acgcctgcaa gcctggcacc gatgaagtcg
     1021 tgccattttc tgcagcggat cagagcctgg tggagtcgct cgcatcgcag gctgccatcg
     1081 ccatcaccag tcgcaacctg ttcacccaga tggaagagct gtttgagtcg ttcatcagcg
     1141 ttatcaatct ggctatcgac gagaagtccc attacaccgg aggccattgc gagcgcgtgc
     1201 cttccctgac gatgatgctg gccgacgcgg tgaatgcaac gcaaaccgga ccgctggcgg
     1261 atttcgtcat gaatgaccgg gaccgctatg aactcaagat cgccggcttg ttgcacgatt
     1321 gtggaaaggt gaccacaccg gtgcatgtgg tggacaaggc gaccaagctg cagaccctgt
     1381 ttgaccggat cgatttgctg gacacgcgtt ttgaagtgct caagcgggac caggaaattg
     1441 cgacgctgcg ggcacagctt gccttgcgcc cggtgcagga tgtggccggg gagaccaacc
     1501 tgcaggcgca gtgtcgccag aaaatcactg cgctggagga ggaccgtgat ttcctgcgcc
     1561 aggtcaacct cggggcggag tccatgccag aggcagacca gcagcgggtt cggcacattg
     1621 gtgcgaccaa tgtgtggcgc aatccggatg gcgtgcaaac tgaatttctg acggctgatg
     1681 aggtggaaaa cctgaccgtg cgttcgggca cattgacggc cagtgaacgt gaaaccatca
     1741 attaccacat tgttgccacc atcaagatgc tgcagcagat gccgtggccc aggcatttga
     1801 ggcatgtgcc cgaatacgcc ggcgggcacc acgagcgcat ggatggcaag ggttacccga
     1861 aggggctcac gcgcgagcag atgtccgttc aagcccgcat catgggtatt gcggatattt
     1921 ttgaggcatt gactgccaag gatcgtccgt acaagcaggg catgaccttg tcgcaggcca
     1981 tggccatcat gcacaagttc agcagaaatg ggcacattga cccggacctg ttcgatgtgt
     2041 ttgtcaagac cggcgtttat cggcgttatg ccgaactgtt tctcgatccg tggcagatcg
     2101 acgaggtcaa tcctgccgcg tggttgccaa agccggcgca atccgcctga tacgttcgcg
     2161 cgcagtggtc gatgcggcaa tccgcagcac ttcggcacgc ggagtccggg ctagcaggtc
     2221 ccaatcactc agtacagtgc gttccaggtt gggcccaagg gtgtggtgac cgggtttgtg
     2281 ggtgtgccca tggatcatgt ggtgcgcatt ggcttccctg agcagggcga tggctgccgg
     2341 ggtatcgatg tcggcatact cggtttccac cgctttgcgg gcttcgcttt ggtcgcgcaa
     2401 agcgcgcgcg atctgctggc gttccgccag tggcttggcc agaaatgcct gctgccatgc
     2461 tgcactgcgc acgctggtgc gaaacgcctg atagggtaag tcgtccaggc acagcgcatc
     2521 tccgtgggtc agtacccagc gctcgccggc aaaggccagc acgcaggggt ctgacagcaa
     2581 ctgcgcgccg caagcggcca tcaactgctg gcccatcagg aaatcccgat tgccgtgcat
     2641 gatgtacacc gggcgccggg cagccgcgtc ggcaatgacc tgggtgcagc gcgcttcaaa
     2701 gctgttggca tcggcgatgg catcgtcacc cacccagact tcaaacaggt cacccaggat
     2761 gaatacggca tcggccgtcg tgccgtgcag gtactgggac caggcatcca aggtcgcggt
     2821 atcggtggcc tgcaaatgca ggtcagaaat gaaatcgacg cacccccatt gcggtggtgc
     2881 gtcgatgtgc atcacaggat tctgaagtat cagagggcta cggctttttc aatcacgacg
     2941 tcttccttgg gcacgtcgtc atgaaagccc ttgcggccgg tttttacggc tttgatcttg
     3001 tccaccacgt cggtgccgga gatgactttg ccaaacacgg cataacccca tccctgctgc
     3061 gatggcgaag tgtggttcag gaagccgttg tcagccacat tgatgaaaaa ctgggcagtg
     3121 gcagaatgcg ggtcgccggt gcgggccatg gccaccgtgt agttggcgtt cttgaggcca
     3181 ttgttggctt cgttctcgat gggtgcatcg caggtcttct gggccatgcc tggctccatg
     3241 ccaccaccct ggaccatgaa tccggggatg acgcggtgga agatggtgtt gttgtagtgg
     3301 cccttgttca cgtaggcaag aaagttggcg acggatttgg gtgctttttc agcgtccagt
     3361 tccagcgtga tgacaccgta gttagcgacg tgaagttcta cttggggttt gctcatgatt
     3421 tatttcacca aagtggcaga gttgataacg ataggggtgg caggcacgtt ttggtacatg
     3481 cccttgttgg tggtggcaac gccgcgaatc ttgtccacgg tgtccatgcc ttgcacaacc
     3541 ttgccgaata cggcgtagcc attgccgtca ggctgaggcg cgttgagtcc ggaattgtcc
     3601 accacattca caaaga
//
LOCUS       DAOSJM010000253         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_22839_length_3580_cov_4.638014, whole genome shotgun sequence.
ACCESSION   DAOSJM010000253 DAOSJM010000000
VERSION     DAOSJM010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22839_length_3580_cov_4.638014"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PKX82_09225"
     CDS             <1..309
                     /locus_tag="PKX82_09225"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOF52475.1"
                     /translation="DPTASYVARLLHKGPDAFLKKIGEEATEVVMAAKDTDHGGDATK
                     IVYEVADLWFHCMIALAHYGLTPADVIAELERREGTSGIEEKALRKAVARDASEGAPT
                     "
     gene            306..713
                     /locus_tag="PKX82_09230"
     CDS             306..713
                     /locus_tag="PKX82_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52476.1"
                     /translation="MSQDFTTPVSPDPATLQKLQSLNTIGTISYVMHLIVAIGAVIPG
                     GQFGPVLLIAALVLDMVKRSDAEGTWHASHFRWRIRTVIIAGVLYLVTAPLWILFLIP
                     GWIAWTAISIWFLYRIVTGFLAMNKGQEMEIPA"
     gene            710..1084
                     /locus_tag="PKX82_09235"
     CDS             710..1084
                     /locus_tag="PKX82_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOF52477.1"
                     /translation="MSADNHVHDDNCLFCKIAARKIPSKTVYEDEEFYAFHDIAPWAP
                     VHFLIIPKAHIVSMAHVGPEDAPLLGRMMALVPKLAAQEGCNPYPDGGFRLVTNTGKE
                     GGQEVHHLHFHVMGGSRPWLRG"
     gene            1121..1360
                     /gene="tatA"
                     /locus_tag="PKX82_09240"
     CDS             1121..1360
                     /gene="tatA"
                     /locus_tag="PKX82_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HOF52478.1"
                     /translation="MGSFSIWHWLIVLLIVVMVFGTKKLKNMGSDLGGAVKGFKDGMK
                     EGSTTAEEKSAAPAQVATNATAEKTTIDVEAKQKS"
     gene            1365..1817
                     /gene="tatB"
                     /locus_tag="PKX82_09245"
     CDS             1365..1817
                     /gene="tatB"
                     /locus_tag="PKX82_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOF52479.1"
                     /translation="MIDLGISKIALIGAVALIVIGPEKLPRVARTVGTLLGKAQRYVA
                     DVKAEVSRSMELEELKKMRDTVENAAKDVENTIQSETSDFEKSWSEATQDALPAAEPE
                     VPQYRHPKKKWRLKQGATPQWYKARNGVRTKALSGAARVARYRPQRLS"
     gene            1839..2633
                     /gene="tatC"
                     /locus_tag="PKX82_09250"
     CDS             1839..2633
                     /gene="tatC"
                     /locus_tag="PKX82_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOF52480.1"
                     /translation="MASEDKTPPDELAGTEQPFVQHLVELRDRLIKAAIAVGVAGAVL
                     ALFPGPAALYDILAAPLVATLPAGGKLIATSVISPFLVPLKIMLMTAFLLALPVVLWQ
                     VWAFVAPGLYSHEKKLVLPLVVSSTVLFFVGVAFCYFFVFGQVFKFIQGFAPTSIAAT
                     PDIESYLDFVLSMFLAFGMAFEVPIVVIVLARMNIVTIEKLKAFRSYFIVLAFVISAI
                     VTPPDVVSQLALAIPMCILYEIGIWAAQIFIKHTQAPPEEAPSSGA"
     gene            complement(2695..>3580)
                     /locus_tag="PKX82_09255"
     CDS             complement(2695..>3580)
                     /locus_tag="PKX82_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOF52481.1"
                     /translation="PWFRFFYGDQGNEAQSGLGSGVIVSPSGYILTNNHVIEGADEIE
                     VFLNDSRHAVAKVIGTDPDSDLAVLKIELDKLPTIVLGSSDNLEVGDQVLAIGNPFGV
                     GQTVTSGIVSAMGRNQLGINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSR
                     SGGSMGIGFAIPVSTAKQVLDGLVKDGQVTRGWIGVEPSDLSPELAETFDVKAKEGVI
                     ITGVLQNGPAAKAGIRPGDVIVSIGEKPVKDVTQLLSMVSGLKPGTATRFVIARKNQN
                     MELDVSPGVRPKPRPQPQ"
BASE COUNT          691 a         1016 c         1094 g          779 t
ORIGIN      
        1 gaccccacgg ccagctacgt cgcgcgcctg ctgcacaagg ggcccgatgc ctttctgaag
       61 aaaatcggcg aggaagccac cgaggtggtg atggccgcca aggacaccga ccacggaggc
      121 gatgccacca agatcgttta cgaagtggcc gacctgtggt ttcactgcat gattgcgctg
      181 gcccactatg gcctgacgcc ggccgatgtg atcgccgaac tggagcgccg cgaaggcacc
      241 agtggcattg aagaaaaagc gctacgcaag gcggttgcgc gtgacgcgag cgaaggagca
      301 cccacatgag ccaggatttc acgacgccgg tatcaccaga cccggccact ttgcagaagc
      361 tgcagtcgct caacaccatt ggcaccatca gttatgtgat gcacctgatc gtcgcaattg
      421 gcgcagtgat tcctggcggg cagtttggcc cggtgttgct gattgcggcc ttggtcctgg
      481 atatggtcaa gcgcagtgat gctgaaggca cctggcatgc atcgcatttc cgctggcgca
      541 ttcgcacggt gatcatcgcc ggtgtgctgt atctggtgac cgcaccgctg tggattctgt
      601 ttttgattcc cgggtggatt gcctggacag ctatttccat ctggtttctt taccgcatcg
      661 tcactggctt ccttgccatg aacaaaggcc aggagatgga gattcccgca tgagtgctga
      721 caaccacgtt cacgacgaca actgcctgtt ctgcaagatt gcagccagga agattccctc
      781 gaagaccgtc tacgaagacg aagagtttta cgcctttcac gacatcgcgc cgtgggcacc
      841 ggtccacttc ttgattattc ccaaagcgca catcgtgtcc atggcccatg tgggtccgga
      901 ggatgcccct ttgctggggc gcatgatggc gctggtgccc aagctggctg cccaggaggg
      961 ttgtaaccct tatccggatg ggggcttccg gttggtcacc aacaccggca aggaaggtgg
     1021 acaggaggtt caccacctgc attttcatgt catgggtggt tcccgcccat ggttgcgcgg
     1081 ctgagtcact agaatacaaa acattcgtta ggagtaattc atgggttcat tttccatttg
     1141 gcattggctg atcgttctgt tgatcgtggt gatggtgttc ggcaccaaga agctcaagaa
     1201 catgggttcg gatctgggtg gtgccgtcaa gggcttcaag gacggcatga aagaaggctc
     1261 caccacggcg gaagagaagt cggcggcacc tgcccaggtt gctaccaacg cgactgccga
     1321 aaaaaccacg atcgacgtgg aagccaagca gaagtcataa ctgagtgatc gacctcggca
     1381 tatcgaaaat agcgctgatc ggtgcggtgg cgttgatcgt catcgggccc gagaagctgc
     1441 cgcgcgtggc gcgtaccgtg ggcactttgc tgggcaaggc gcagcgctac gtggccgacg
     1501 tcaaggccga agtcagccgc tcgatggagc tcgaagagct caagaagatg cgtgacaccg
     1561 tcgagaacgc tgcgaaggac gtggaaaaca cgatccagtc ggaaaccagt gatttcgaaa
     1621 aatcctggtc cgaagcaaca caggatgcct tgcctgcagc cgagccggag gtgccgcagt
     1681 accggcatcc caagaagaaa tggcgcctca agcagggcgc cacgccgcag tggtacaaag
     1741 cccgcaatgg cgtacgcacc aaagccctgt ccggcgctgc ccgtgtggca cggtaccgcc
     1801 cgcaacggct cagctagaac acagagtttt caccacccat ggcttctgaa gataaaacgc
     1861 cgcccgatga actggcgggt actgagcaac cctttgtgca gcacctggtg gagctgcgcg
     1921 accggttgat caaggcggct attgcggtgg gtgtggctgg cgcggtgctg gccctgtttc
     1981 ccggcccggc tgcgctgtat gacattctgg ctgcaccgtt ggtggccaca ctgccagccg
     2041 gtggcaagct gattgccacc tcggtgatct cgccgtttct ggtgccactc aagatcatgc
     2101 tgatgacggc ctttctgctg gcgttgccag tggtactctg gcaggtctgg gcctttgtcg
     2161 cgccggggct ttattcgcat gaaaagaagc tggtgctgcc gctggtggtg tccagcacgg
     2221 tgctgttttt tgtgggcgtg gcgttttgct acttcttcgt gtttgggcag gtgttcaagt
     2281 tcatccaggg ttttgccccc acgagcattg ccgctacacc cgacatcgag tcctacctgg
     2341 attttgtgct gtccatgttc ctggcttttg gcatggcgtt tgaagtgccg atcgtggtga
     2401 tcgtgctggc ccgcatgaat attgtgacga tcgaaaagct caaggcgttc cgcagctatt
     2461 tcatcgtgct ggcgtttgtg atctcagcca tcgtgacgcc gcccgatgtg gtgtcgcagc
     2521 tggcgttggc gattccgatg tgcatcttgt atgagattgg catctgggcc gcccaaatat
     2581 tcatcaagca cacccaggca ccgcctgaag aggctccctc ttcgggggct tgagagttaa
     2641 ataggccgct agcccccgtt tttattgggg gtttcgctac tgaaattgta gcaattactg
     2701 tggttgtggc cggggcttgg gacgcacacc cgggctcaca tccagttcca tgttctggtt
     2761 cttgcgggca atcacaaagc gtgtggccgt gcccggcttg aggcccgaga ccatggacag
     2821 caactgggtc acatccttga cgggcttctc cccaatgctc acgatcacat cgccagggcg
     2881 aatgcccgct ttggctgccg ggccgttttg cagcacgccg gtgatgatca cgccttcctt
     2941 ggctttcaca tcaaaggttt cggccagttc cggggaaagg tcgctgggct ccacgccaat
     3001 ccagccgcgg gtgacctggc cgtccttcac caggccatcg agcacctgct tggcagtcga
     3061 caccggaatg gcaaagccaa tgcccatgct gccacccgag cgggaataga tggcggtatt
     3121 gatacccagc agattgccat tggtgtccac cagtgcgccc cccgagttgc cggggttgat
     3181 ggcggcgtcg gtctggatga agttctcaaa cgtattgata cccagctggt tacggcccat
     3241 ggcactcaca atgccgctgg tgacggtctg gcccacgcca aacgggttgc cgatggccag
     3301 cacctggtcg cccacttcca ggttgtcgga gctgcccagc acgatggtgg gcagcttgtc
     3361 gagttcgatc ttgagtacgg ccaggtcgct gtcggggtcg gtgccgatca ccttggccac
     3421 ggcgtggcgg ctgtcgttga ggaagacctc aatctcgtca gcgccttcaa tcacatggtt
     3481 gttggtcagg atgtagccgc tggggctaac gatcacgccg ctgcccaggc cactttgcgc
     3541 ctcgttgccc tggtcgccat agaaaaagcg gaaccagggg
//
LOCUS       DAOSJM010000254         3563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_22973_length_3563_cov_5.804447, whole genome shotgun sequence.
ACCESSION   DAOSJM010000254 DAOSJM010000000
VERSION     DAOSJM010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22973_length_3563_cov_5.804447"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PKX82_09260"
     CDS             complement(<1..1214)
                     /locus_tag="PKX82_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52482.1"
                     /translation="MADTQQKIAIQAAIHAFTTKSLHDAATALFACLGYKSDRTIAVD
                     SVEAFCHQFDPSDLLAHPRAQKASWKSIELLFQLTDEELSRNATLFKDTAIRASLLQS
                     YVFFAVELQPGDYARGKLAAITRQINRVFPMPVMVLFKVAGNLSIAVINRRQNKRDES
                     KDVLGKVTLIQNISTVKPHPGHLDILASFSTTELLARKKPIGNFDQLHAAWEDVFNVE
                     LLNKRFYEELSNWYFWARKQVSFPADLEPDVDTRNATSVIRLLTRLIFCWFLKEKDLI
                     PDHLFDQNAVASALNSVGEDESTYYHAILQNLFFATLNQPMNADGEPVRQFAADGDFQ
                     ENRNQHGVKTLYRYKKSFRDPTSALGLFEDIPFLNGGLFACLDTEDDTGKVQYADGFS
                     RNPKKQPVVPNRL"
     gene            complement(1217..1627)
                     /locus_tag="PKX82_09265"
     CDS             complement(1217..1627)
                     /locus_tag="PKX82_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOF52483.1"
                     /translation="MTLRYLLDTNIVSYYLRRSSNALEERVNDALKHQTIAISALTRA
                     ELRFGQEGMGIEDRRRGLIDHFLLRLPNIAWTNQAADHYGEIKTLHKRQGTPIGELDT
                     QIAAHALAEDLILVTHNTRHFEKVPGLQLEDWMN"
     gene            complement(1624..1881)
                     /locus_tag="PKX82_09270"
     CDS             complement(1624..1881)
                     /locus_tag="PKX82_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOF52484.1"
                     /translation="MLATAKVFTTGNSQAIRLPKAFRVDTREMWITKNEGTGEIILKP
                     KPAPDELEAFLAELKTMPVSDEFIPPRDDAPRPSPFEDWPE"
     gene            complement(1917..>3563)
                     /locus_tag="PKX82_09275"
     CDS             complement(1917..>3563)
                     /locus_tag="PKX82_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HOF52485.1"
                     /translation="KDQYQGETNVRNFSQENREKFLIGMMKVNFLKRLESSVKSFAIT
                     MERTVNKIEDLQARLGAYKKLRDGKPEDLQASLFLMEAEEDDELREAFEVGTLKYRME
                     HMDVDRWLADLTADKQQLSMLAESADGVGPERDAKLEALKTIISTKVTQPSTNNRGEA
                     NRKVIVFCAFADTAAYLYEQLEDWAMQTLKVNIALVSGGTRPNRATLGAPEFNQILTN
                     FAPRAKQRAKMRSMPQGSEIDILIATDCISEGQNLQDCDLLVNYDIHWNPVRIIQRFG
                     RIDRIGSLNAQIQLVNFWPTPDLNKYINLKNRVEARMALVDIAATNEDNILTNSELEE
                     LIQQDLKYRDKQLMRLKDEVLDLEDFNESVALNAFTLDDFRMDLSNYVEVNRTRLQDA
                     PYGLYGVVPSTTASAEAASVKPGVIYCLRQQHQQTQGVEAVNPLNPYFLVYIQADGTV
                     RYNFTAPKQVLEIFRALCQGKTAPYADLCKLFDAQTSHGQDMSAYSSLLDQAVAAIVA
                     QFGRKNAANLFSGRGGKLMDAGKTVKSNNDFELITWLVIK"
BASE COUNT          654 a          922 c         1119 g          868 t
ORIGIN      
        1 aggcggttgg ggaccaccgg ctgtttcttg ggattgcgtg aaaaaccgtc ggcatactgc
       61 accttgccgg tgtcgtcttc cgtgtcaagg caggcgaaca ggccaccgtt caggaatgga
      121 atgtcctcga acaagccaag cgcactagtg gggtcgcgga aagacttttt gtagcggtag
      181 agggtcttga cgccatgctg gttgcggttt tcctgaaaat cgccatccgc cgcgaactgc
      241 ctgacaggtt cgccatcggc attcatgggc tggttgagcg tggcgaaaaa caggttttgc
      301 agaatggcgt ggtaataggt gctctcatct tcaccgacag aattcaaggc gctggcgacg
      361 gcattttggt caaacaaatg atcaggaatg aggtcttttt ctttcaggaa ccagcagaag
      421 atcagccggg tcaataggcg gatcacgctt gtggcgttgc gggtgtcaac gtccggctcc
      481 agatcggcgg ggaagctgac ttgcttgcgt gcccagaaat accagttgga tagttcttcg
      541 taaaaacgtt tgttgagtag ttcaacgttg aatacgtctt cccacgcggc gtggagttgg
      601 tcgaagttgc cgatgggctt cttgcgggca agcagttccg tggtcgagaa cgaagccaga
      661 atgtccaggt gacccggatg cggtttgact gttgagatgt tttggatcag cgtgaccttg
      721 cccaagacat ctttgctttc atcgcgcttg ttttgcctgc ggttgatgac ggcaatggac
      781 aaattacccg ccaccttgaa cagcaccatg accggcatgg ggaaaacgcg gttgatctgg
      841 cgggtgatag cggccagttt gccccgtgcg taatcacccg gctggagctc cactgcaaag
      901 aacacatagg actgaagcag ggacgctctg atggcggtgt ctttgaacag cgtggcgttg
      961 cgcgaaagtt cctcgtcagt gagctgaaag agaagctcaa ttgacttcca gctggctttt
     1021 tgcgcgcggg ggtgggcaag caagtcgctc gggtcaaact gatgacaaaa agcttccacc
     1081 gagtcaacgg caatcgttcg gtcagatttg tagccaagac aggcaaacaa cgcggtcgcc
     1141 gcgtcatgca acgatttggt tgtgaatgca tgaatggcgg cttgaatcgc tattttttgc
     1201 tgcgtgtcgg ccatgctcaa ttcatccagt cttcaagttg caatcccgga accttttcaa
     1261 agtggcgggt gttgtgggtg accaggatca ggtcttcggc cagcgcgtgg gcggcgatct
     1321 gggtgtccag ctcgccaatc ggcgttccct ggcgcttgtg cagggttttg atctcgccgt
     1381 agtgatcggc ggcctggttg gtccatgcga tgttgggcaa gcgcaagagg aaatgatcaa
     1441 tcaggcctct tcgccgatct tcgatgccca tgccctcttg gccaaagcgc agttccgccc
     1501 gtgtcagtgc agaaatggca atggtctggt gtttcagtgc atcgttgacc cgctcctcca
     1561 gcgcatttga gctgcgccgc aggtagtagc tgacgatgtt ggtgtccagc aggtagcgga
     1621 gcgtcattct ggccagtcct caaaggggct tggacggggc gcatcgtcgc ggggggggat
     1681 gaattcatcc gagaccggca tcgtcttcaa ctccgcaaga aaggcctcca gctcatccgg
     1741 cgccggtttg ggcttcagga tgatttcacc ggttccttcg ttcttggtga tccacatttc
     1801 acgggtgtcc accctgaacg cctttggtaa acgaatggct tggctgttgc ctgtagtaaa
     1861 cactttggcg gttgcaagca tggctggcct cctgaatagt agatatgaaa acgtatctac
     1921 tttatcacca gccaggtgat cagctcaaag tcgttgttgc ttttcaccgt tttgccggca
     1981 tccatgagct tgccgccccg gcccgagaac aagttggcgg catttttgcg accgaactgc
     2041 gccacgatgg cggccacggc ctggtccagc aggcttgaat acgcgctcat gtcttgcccg
     2101 tggctggttt gggcatcgaa cagcttgcac aagtcagcgt agggcgcggt cttgccctgg
     2161 cacagggcgc ggaaaatttc cagcacttgt ttgggcgcgg tgaagttgta gcgcacggtg
     2221 ccgtcggcct ggatgtagac cagaaagtag gggttgagcg ggttcaccgc ttcaacccct
     2281 tgggtttgct ggtgctgctg gcgcaggcag tagatgacgc ctggcttgac ggaggcggct
     2341 tcggcgctgg cggtcgtact gggcaccacg ccatacaggc cataaggcgc gtcctgcagc
     2401 cgggtgcggt tgacttccac atagttgctc aggtccatgc ggaagtcgtc cagcgtgaac
     2461 gcattcaggg ccacggattc gttgaagtct tccaggtcca gcacctcgtc tttcaggcgc
     2521 ataagctgct tgtcgcggta cttcaggtct tgctggatca gttcttccag ctcgctgttg
     2581 gtgaggatgt tgtcttcgtt ggtggcggca atgtccacca gcgccatgcg cgcctcgacg
     2641 cggtttttca ggttgatgta cttgttcaag tcgggcgtgg gccagaagtt gacgagctga
     2701 atctgggcgt tgaggctgcc gatgcggtcg atccggccga agcgctggat gatccgcacc
     2761 gggttccagt gaatgtcgta gttgaccagc aagtcgcagt cttgcaggtt ctggccctcg
     2821 gagatgcagt cggtggcgat caggatgtcg atttcgctgc cctgcggcat gctgcgcatt
     2881 ttggcgcgct gcttggcgcg cggggcgaag ttggtgagga tctggttgaa ctcaggcgcg
     2941 cccagggtgg cccggttcgg gcgtgtgccg ccagagacga gggcgatgtt gaccttgagc
     3001 gtctgcatgg cccagtcctc aagctgctca tatagatagg cggcggtgtc ggcgaaggcg
     3061 cagaagacga tgaccttgcg attggcttcg ccccggttgt tggtgctggg ctgggtgact
     3121 ttggtgctga tgatggtttt gagcgcttcc agcttggcgt cgcgctcggg gccaacacca
     3181 tcggcgcttt cggccagcat ggacagttgc tgcttgtctg cggttaggtc ggccagccag
     3241 cggtctacgt ccatgtgttc catgcggtat ttgagggtac ccacctcgaa cgcttcgcgc
     3301 aattcgtcgt cttcttcggc ctccatcaga aacagactgg cctgcagatc ttcgggcttg
     3361 ccgtcccgca gcttcttgta ggcgccaagg cgcgcctgca ggtcttcaat cttgttgacg
     3421 gtgcgttcca tggtgatggc aaaagacttg acggaactct ccagccgttt gagaaagttg
     3481 accttcatca ttccgatcag gaacttctca cggttctcct ggctgaagtt gcggacgttg
     3541 gtttcgccct ggtattggtc ttt
//
LOCUS       DAOSJM010000255         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23104_length_3546_cov_4.663420, whole genome shotgun sequence.
ACCESSION   DAOSJM010000255 DAOSJM010000000
VERSION     DAOSJM010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23104_length_3546_cov_4.663420"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PKX82_09280"
     CDS             <1..356
                     /locus_tag="PKX82_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF52486.1"
                     /translation="EQLIQTVLNIAHNACQALTERIAAGDANLTFRTRVARQVTFGKQ
                     RYRLALELHVIDNGPGVPDSIKDRIFYPLVSGREGGSGLGLTLAQTFVQQHHGLIEVD
                     SVPGRTDFKILIPLP"
     gene            385..1893
                     /gene="ntrC"
                     /locus_tag="PKX82_09285"
     CDS             385..1893
                     /gene="ntrC"
                     /locus_tag="PKX82_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HOF52487.1"
                     /translation="MKPIWIVDDDQSIRFVLEKALLREHLPTRSFSNPRDVLTALSTA
                     SDEDGPQILVSDIRMPGGSGLDLLEKVKAKHPGLPVIIMTAFSDLDSAVSAFQGGAFE
                     YLPKPFDLPKAVELIRRAVEESQREEVAEERMAETPEMLGQAPAMQDVFRAIGRLSQS
                     NVTVMITGESGSGKELVARALHKHSPRANGPFVAINTAAIPKDLLESELFGHERGAFT
                     GAQTMRRGRFEQADGGTLFLDEIGDMPFELQTRLLRVLSDGHFYRVGGHSAVKSNVRV
                     IAATHQDLEQRVKEGVFREDLFHRLNVIRLRLPALRERKEDVSMLTRHFLQQSAKQLG
                     VEPKRISDAALAWLESFGFPGNVRQLENICHWLTVMAPAQVIEPKDLPPEVGVSNHEG
                     GAQVLAPVVMESVAATTVALPVVAPTAPSTAPAVAPGGAMENWESALEAEAVALLDSG
                     SPEVWDTLTRRFESRLILAALGSTKGRRIEAAQKLGIGRNTITRKIQELGLE"
     gene            1961..3391
                     /locus_tag="PKX82_09290"
     CDS             1961..3391
                     /locus_tag="PKX82_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF52488.1"
                     /translation="MGSAFVVSWIGALVCFGAGVLLGRVLYGRGRTASSNLDDNTSLA
                     PVFQPQDDEALRKRNAIFAKVFNLVPETLTITRIADGRYVEVNHNWQPMIGYAREDAI
                     GHTSAELNVWAEPEQRQRIVETIRRDGEIHGVDATFKHKAGHLLYAKVSASIFDSDGE
                     KYMMLAVQDVTAEREAQQQIVELNQQLEQRVQQRTLKLEQANAELATTLQTLRYAKDE
                     LVKTDKLAALGSLVAGVAHELNTPIGNSLTVATTLDHRVKTLDGQLTTGLRRSDLDGF
                     VADAKFAMDVLVRNLMRAGDLIRSFKQVAADRASAQRRVFQLQELVSETLITLNPSIR
                     RCRCEVKLEIDQGIELDSYPGPLGQVLDNLINNALIHGFADGGEGCIWISGRSAGAAV
                     VTLSVRDNGVGIPLENQSRIFDPFFTTRLGLGGSGLGLHIVYNIVTDILGGSIEVQSV
                     PGAGATFLLHLPLRTPEPDANAQPAG"
     gene            complement(3376..>3546)
                     /locus_tag="PKX82_09295"
     CDS             complement(3376..>3546)
                     /locus_tag="PKX82_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOF52489.1"
                     /translation="DFGFKRNRGLRIDHILVSNALKPLVASCEIDKTPRKNERPSDHA
                     PVVVEIADQPAG"
BASE COUNT          709 a         1019 c         1107 g          711 t
ORIGIN      
        1 acgagcaact gatccagacc gttctcaaca tcgcgcacaa cgcctgtcag gcgctgactg
       61 agcgtatcgc cgcgggtgat gccaacctca catttcgcac gcgcgtggcc cgacaggtga
      121 catttggcaa gcagcgctac cggttggcat tggaattgca tgtcattgac aacggacctg
      181 gtgtgccaga ttcgatcaag gaccgcattt tctatccgct ggtgtcgggc agggaaggtg
      241 gttccgggct ggggctgaca ttggcgcaaa cttttgttca gcagcaccac ggattgatcg
      301 aggttgacag cgtgccaggc cgtacggatt tcaaaatttt gattccatta ccgtaacgtg
      361 caagtaccaa gggaccacca gaacatgaag ccgatttgga tcgtagatga tgaccagtcc
      421 atccgcttcg tgctggagaa ggcgctgctg cgcgaacacc tgcccacacg cagtttctcc
      481 aatccacgcg atgtgctgac tgcgctgagc acagcatccg atgaagacgg cccccagatt
      541 ctggtgagcg acatccgcat gccaggtggc tccggtctgg acttgctgga aaaggtcaag
      601 gccaagcatc ctggtctgcc ggtcatcatc atgacggcgt tctccgatct ggatagtgcg
      661 gtgagtgcat tccagggcgg agccttcgag tacttgccca agccgtttga tttgcccaag
      721 gcggtggaac tgatccgccg cgccgtggaa gaaagccagc gcgaggaagt cgccgaagag
      781 cgcatggccg aaacccccga gatgctcggt caggcgcccg ccatgcagga cgtgttccgt
      841 gccatcgggc gactttcgca aagcaatgtg acggtgatga ttaccggcga gtccggttcc
      901 ggcaaggaac ttgtggctcg ggcactgcac aagcactcgc cccgcgccaa tgggcctttt
      961 gttgctatca atactgcagc aatcccgaag gatttgctgg aaagcgagtt gtttgggcac
     1021 gaacgtggtg catttaccgg cgcgcagacc atgcgccgcg gccgctttga gcaggccgat
     1081 ggcggcacgc tatttctcga cgaaatcggc gacatgcctt ttgagttgca gacccgcttg
     1141 ctgcgtgtgc tgagcgacgg gcatttttac cgggtgggtg gtcacagcgc tgtcaaatcg
     1201 aatgtgcggg tgattgccgc cacccaccag gacctggagc agcgcgtcaa ggaaggtgtg
     1261 ttccgggagg acttgttcca ccgcctgaac gtcattcggc tgcgcctgcc ggccctgcgc
     1321 gaacgcaagg aagatgtgtc gatgctgacg cgtcacttcc tgcaacaaag cgccaaacag
     1381 ttgggtgtgg aacccaagcg catctcggat gccgcactgg cctggctgga gtcttttggc
     1441 tttccgggca atgtgcggca actggagaac atctgccatt ggctgaccgt catggcgccc
     1501 gcgcaggtga tcgagcccaa ggacctgcca cccgaagtgg gcgtatccaa ccacgaaggg
     1561 ggcgcacagg tcctggcgcc cgtggtgatg gagtccgttg cagccaccac ggtggcgctg
     1621 ccagtggttg ctccgacagc gccatccact gcgcccgcag tcgcgcccgg tggtgccatg
     1681 gagaactggg agtcggcact ggaggctgaa gcggtggctt tgctggattc cggaagtccc
     1741 gaggtgtggg ataccctgac gcgccgcttc gaatcgcgtt tgatcctggc cgctctgggc
     1801 agtaccaagg gccgtcgtat tgaggctgcc caaaaactgg gtatcggacg caacaccatc
     1861 acgcgcaaga tccaggaact cggtctcgaa taggccttca gtccattgtc agcatcggct
     1921 ggacttcgac acttcataat ggcgacagaa ggagtggctg atgggcagtg cattcgtcgt
     1981 ctcgtggatc ggcgcattgg tatgttttgg cgcgggcgtg ctgctggggc gtgtgttgta
     2041 cgggcgtggc cgtacggcgt catccaacct tgatgacaac acgtctctgg cgccggtgtt
     2101 tcagccgcag gacgatgagg ctctgcgcaa gcgcaatgcc atctttgcga aggtgttcaa
     2161 cctggttccg gaaaccctga cgatcacgcg tattgctgac gggcgttacg tggaggtcaa
     2221 tcacaattgg cagccaatga ttgggtatgc gagggaagac gcgatcgggc acacgtcagc
     2281 agaactcaat gtctgggccg aacccgaaca acgccagcgc atcgtagaaa cgatccgtcg
     2341 cgatggagag attcatggcg tcgatgcgac gttcaagcac aaggccgggc atctgctgta
     2401 tgcgaaagtt tcggccagca tctttgactc cgatggcgag aaatacatga tgctggcagt
     2461 gcaggatgtc accgccgagc gcgaagccca gcagcagatt gtcgaactga accagcaact
     2521 ggagcagcgt gtccagcagc gcaccctcaa gctggagcag gccaatgccg agctggccac
     2581 cacattgcag accttgcgct acgccaagga tgagctggtc aagaccgaca agctggccgc
     2641 actgggctct ttggtcgcag gtgtggccca tgaactcaac accccgattg gcaacagcct
     2701 gaccgtggcc accacgctgg accaccgtgt caagacgttg gatggtcagc tgaccacggg
     2761 cctgcgccgc tccgacctgg atgggtttgt ggcagatgcc aagtttgcga tggatgtgct
     2821 ggtgcgcaat ctgatgcgcg ccggcgacct gatccgcagt ttcaagcagg tggctgccga
     2881 ccgtgccagc gcgcagcggc gcgtgttcca gttgcaggaa ctggtgtcag agacgttgat
     2941 cacattgaac ccctccatcc ggcgttgccg atgtgaagtc aagctggaga ttgaccaggg
     3001 cattgaactc gacagctatc cgggtccgct cggacaggtg ctggacaacc tcatcaacaa
     3061 tgccctgatc catggctttg ctgatggcgg ggaagggtgt atctggatat ccggccgatc
     3121 cgcaggcgcg gccgttgtca cgctctcggt gcgcgacaac ggtgtaggca taccgctgga
     3181 aaaccagagc cggatattcg atccattctt caccacccgc ctggggctgg gcggctcggg
     3241 tctggggctg catatcgttt acaacatcgt gacagatatt ctcggcggca gcattgaggt
     3301 gcaaagcgtg cccggcgctg gggccacttt cctgctgcac cttcccctgc gtacgcccga
     3361 gcccgacgca aacgctcagc ccgcaggctg atcggcaatc tccaccacca ctggcgcgtg
     3421 gtcgctggga cgctcgtttt tccgtggagt cttgtcgatc tcgcacgaag ccaccaacgg
     3481 tttgagtgca ttgctcacca gaatgtggtc gatgcgcagg ccacggttgc gtttgaatcc
     3541 gaagtc
//
LOCUS       DAOSJM010000256         3545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23116_length_3545_cov_3.731805, whole genome shotgun sequence.
ACCESSION   DAOSJM010000256 DAOSJM010000000
VERSION     DAOSJM010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23116_length_3545_cov_3.731805"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PKX82_09300"
     CDS             complement(<1..1079)
                     /locus_tag="PKX82_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52490.1"
                     /translation="MTDITPTRERLAKPALEYPFAQAPETGKTLEVARGVFWIRMPLP
                     MTLDHINIWALEDGDGWTIVDTGMRTDDTLAAWRALFASATDKRPLQRVIGTHMHPDH
                     IGMSGWLTRKFHCRLWMTQLEYLNCRVLTGDTGREAPEDAVAFYQKAGWGQGAIENYQ
                     ARFGNFGKHIHALPESYRRIQDGEDIQIGGSSWRVIVGRGHSPEHACLYSSEKKVLIS
                     GDQVLPRISSNVSVYPTEPDANPMQDWLETLKKIKEEVPDDVLVLPSHNECFRGLHAR
                     LDYLAMAQERTLDRLRKTLKEPRRAIDVFVALFGRSIDETDGGLLNLATGESIACLNY
                     LLHLGEITRQADHEGGGSSDEQRGGA"
     gene            1270..2085
                     /locus_tag="PKX82_09305"
     CDS             1270..2085
                     /locus_tag="PKX82_09305"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HOF52491.1"
                     /translation="MDPDHYVGQSLDLGFRNLFGGHILGQALVAAGNTCEDRDVHSLH
                     AYFIRGGDPRIPIHYNVDRIRDGASFSVRRVTASQDGKAILILSSSFQVDEAGFDHQI
                     AMPVVPPPEAVPLAVTSTRPVPELIPTSSEHLVAHEFAIEMRPVEPVDSQFPDRKEPF
                     QHIWFRAAGPVPGNAALQKCLLAYASDFSLLSTCLRPHGVTYYQPEMVTASLDHAMWF
                     YRDFRMDDWHLYVCDSPSAGHARGLNRGNIFSHDGRLVACVSQEGLIRSTQFR"
     gene            2223..2843
                     /locus_tag="PKX82_09310"
     CDS             2223..2843
                     /locus_tag="PKX82_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006750228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOF52492.1"
                     /translation="MYPGERLNSITHLVGAALAIAGLSILLTISILQKDVLKVVSFGI
                     YGFTLVLLFGMSTIYHSIKNRRAKTILQKMDHNSIYLMIAGSYTPIALVTLQGPWGWT
                     LFGLSWGLALFGIAQEMTLGKRTRRLSMVLYILMGWLVLVAIKPLTQAMPVAGLAWLV
                     AGGVIYSAGIFFYLHDERRKHFHGIWHLFVLGGSFCHFLCFLVYVA"
     gene            2876..3121
                     /locus_tag="PKX82_09315"
     CDS             2876..3121
                     /locus_tag="PKX82_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52493.1"
                     /translation="MPATELTVRSAGMVAGKELLVPTGTHGSTFPHIQDWVTSKLKAK
                     TPVKDVSTSVLVKGIKQWAAYEEKTASRRTLTVFKIT"
BASE COUNT          739 a         1021 c          975 g          810 t
ORIGIN      
        1 gcaccacccc gttgttcatc gctgctgccg ccaccctcgt ggtcggcctg acgggtgatt
       61 tcgccaaggt gcaagaggta gttcaggcag gcaatcgact cccccgtggc aaggttcaac
      121 aacccgccat cggtttcgtc gatgcttcgc ccgaagagcg caacaaaaac atcaatggcc
      181 cttctgggct ccttgagcgt cttgcgcaga cggtccaacg tgcgttcctg cgccatggcc
      241 agataatcca gtcgcgcatg caggccccgg aagcactcgt tgtgggaagg tagtaccagc
      301 acgtcgtcag gcacttcctc cttgattttt ttcagagtct ccagccagtc ctgcatgggg
      361 ttggcatccg gctcggtcgg gtagacggat acgttcgatg agatcctggg cagcacctgg
      421 tcgccggaaa tcaatacctt tttttcggag gaatagagac aggcgtgctc cggggagtgc
      481 cctctgccaa cgatgacgcg ccaggagctg cctccgatct ggatgtcttc cccatcctgt
      541 atgcggcgat agctttctgg cagggcgtgg atgtgcttgc caaaatttcc aaagcgggcc
      601 tggtagttct ctatcgcccc ctgaccccaa cccgcttttt ggtagaacgc gacggcatcc
      661 tcgggggcct cccggccggt atcgccggtc aacacgcggc agttcaaata ttccagctgg
      721 gtcatccaca agcggcagtg gaatttgcgc gtcaaccacc ctgacatccc gatgtggtct
      781 ggatgcatgt gcgtgccaat gacgcgttgg agtggacgct tgtccgtggc gctcgcgaac
      841 aaagctcgcc aggctgcgag cgtatcgtcc gtgcgcattc cggtatccac gatagtccag
      901 ccatcaccat cctccagtgc ccagatgttg atgtgatcca gtgtcatcgg caatggcatg
      961 cggatccaga acacgcctct ggcgacttcg agtgtctttc cggtttccgg cgcctgggca
     1021 aaagggtatt ccagcgctgg ctttgccaga cgctcccgcg taggtgtgat gtcagtcata
     1081 gtgcgactcc agtgtattcg cgttgcccgc gcacgccttc atgacacaaa cgcgcttctt
     1141 acaattggcg catcagttcc catatattga atccagatgc aactacaaga cacgaacaca
     1201 cccagtgttc ctcattccgc gcagggagcc gcccttcagc aactgcttgg cctgttcgaa
     1261 ttggaccgca tggaccctga ccattacgtg ggacagagtc tggacctggg ctttcgcaac
     1321 ctgttcggcg ggcacattct ggggcaggcg cttgttgcag cgggcaacac ctgtgaggat
     1381 cgtgatgtgc actcgctgca tgcctatttc attcgcggcg gagatccccg cattcccatt
     1441 cattacaacg tagaccgcat ccgtgatggt gccagcttca gtgttcgcag agtgaccgca
     1501 tcccaggatg gcaaggccat tctgatcctc tcctcttcat tccaggtcga tgaggccggc
     1561 tttgaccacc agattgcaat gcctgtcgta cctccaccgg aagcggtccc ccttgcagtg
     1621 accagtacgc gtcccgtgcc ggagctgata ccaacgtcga gcgagcattt ggtcgcccac
     1681 gaatttgcca ttgagatgcg ccctgtcgaa cccgttgact cgcagttccc ggacaggaaa
     1741 gagccgttcc agcacatctg gttccgcgcc gccggtcccg ttccggggaa tgcagcgctg
     1801 caaaagtgcc ttcttgcgta tgcctccgac ttcagtctgc tgagcacctg cctgcgaccc
     1861 catggggtaa cttactacca gccggagatg gtgacagcca gtctggacca cgccatgtgg
     1921 ttctatcgtg atttccggat ggatgactgg cacctctatg tctgtgacag tcccagcgca
     1981 ggccatgcac gcggcctgaa ccgcggaaac atcttcagcc acgacggtcg tctggtggca
     2041 tgcgtgagcc aggaggggct gatccgctcc acccaattcc gctgaacgcg aactgtcttt
     2101 agagaagcct cccagtgccg tgcaccactg gccaatggtg taatgcgctg tccctgatcc
     2161 agaccctttg catcgttctt gatgctggac ggcgggtgta cacaaactcc aaagaggcga
     2221 ccatgtatcc tggagaaaga ctgaacagca tcacgcacct cgtcggggct gcgcttgcca
     2281 ttgcaggcct gtccattttg ctgaccatct ctatcctgca aaaagacgtg ctgaaagtcg
     2341 taagctttgg catttatggg tttacgctgg tgctgctgtt cggcatgtcc accatctatc
     2401 acagcatcaa aaatcgtcgg gccaagacca ttctccagaa aatggaccac aactccatct
     2461 atctgatgat cgcgggcagt tataccccca tcgcgttagt gaccttgcag ggtccctggg
     2521 gctggaccct gtttggtttg agttggggac tggccctctt tggtatcgcc caggaaatga
     2581 ctttggggaa gcgcacccgg cgcctctcga tggtgctgta catcctcatg ggatggctgg
     2641 tactggttgc catcaaaccg ctgacacagg cgatgcctgt cgctggactt gcctggctgg
     2701 tggcaggtgg tgtcatctac agtgctggaa ttttctttta tctccacgac gagcggcgca
     2761 aacactttca tgggatctgg catcttttcg tattgggggg tagcttttgc cattttctgt
     2821 gctttcttgt ctatgttgcc tagacaattc accacgccaa gaggggagta cccatatgcc
     2881 tgcaactgag ctcacggttc gatcagccgg catggtggcg ggcaaggaat tgctcgttcc
     2941 aacgggtacg catggaagca catttccgca cattcaggat tgggtgacca gcaagcttaa
     3001 agcgaaaact ccggtgaaag atgtcagcac atccgtcctg gtcaagggta tcaagcaatg
     3061 ggcagcatat gaagaaaaga cagcctccag gagaacactg actgttttca aaattaccta
     3121 gtgtgcgagg gctactttcc actgagtctg acgaatatgt tcacaacaac tttcaccggc
     3181 ggtcgcatca ggcaggccta ctacgatccc gtttcgcgcc agttggattt acattgggac
     3241 accaaggcca tactcgccta caaacacgtt ccgcaagaag tgtatagacg tctgtgtagt
     3301 gcgcccaatc cagccagcta ctgggaggag cggattgcgg atgagtatcc gaaagggacc
     3361 ccaacaactg ccagattcgc tccagatgga gcgagaaagc tcagtgggtt gtttggtgtg
     3421 gatgactgac tgattggata atcgacatac aggtcgctgc gctgaaatca atggcctggc
     3481 gagtcaagta aaatagcggc atgagcaaac cttcgagcaa gacaacgata ctggacgacg
     3541 gatta
//
LOCUS       DAOSJM010000257         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23321_length_3518_cov_4.359226, whole genome shotgun sequence.
ACCESSION   DAOSJM010000257 DAOSJM010000000
VERSION     DAOSJM010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23321_length_3518_cov_4.359226"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /gene="malQ"
                     /locus_tag="PKX82_09320"
     CDS             <1..1113
                     /gene="malQ"
                     /locus_tag="PKX82_09320"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOF52494.1"
                     /translation="DRTALAEFTRLHAAWLDDYALFMVLEDRHGAPWTQWPAALAKRA
                     PEALHLLQKEEAHAIGFWHFVQWRFDVQWQRLRRDAHARGIHIVGDVPIFVAHHSADV
                     WAHADQFLLDGHGDPLVVAGVPPDYFSATGQRWGNPLYHWDTMARNGYHWWKQRMSHL
                     FSQVDVARLDHFRGFETYWEIPATESTAQGGQWRNGPGNAFFAALRKELGALPIIAED
                     LGLITEAVSRLRLDCGFPGMRVMQFAFGDTAHNPYLPHNFEPGTVAYTGTHDNDTTIG
                     WWQQAMESERHAARQYLGPQTDQEIHWAMIQSLSQSVARTLVIPFQDVLGLDGRHRMN
                     TPGHPQGCWTWRFHWSEVNDLPAARLAAMSRAHGRT"
     gene            1167..2456
                     /gene="glgC"
                     /locus_tag="PKX82_09325"
     CDS             1167..2456
                     /gene="glgC"
                     /locus_tag="PKX82_09325"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOF52495.1"
                     /translation="MDKNAPHQLARRTIALVLAGGRGSRLHALTDRRAKPAVYFGGKF
                     RIVDFALSNCMNSGVRRIGVVTQYKSHSLLRHLQRGWSFLRNEFNEFIDLLPAQQRLD
                     ESSWYQGTADAVFQNLDILRAHKPEYILILAGDHIYKMDYANMIADHVAQGARCTVAC
                     IEVPINEAKVFGVMSTDAQRRIVRFDEKPSQPTPMPGRPDMALASMGVYVFDADYLYA
                     ALKADAQSTSSSRDFGKDLIPAIVARGEAVAHPFGQSCIKSSPQAPAYWRDVGTIDAY
                     WAANLDLTNTIPELDMYDRDWPIWTYQEQLPPAKFVFDDDGRRGMVVDSLVSGGCIIS
                     GASARRSVLFSKVRLHSYSTVEEAVLLPEVDVGRNCHLRKVVIDTGCQIPAGMSIGFD
                     AAEDARRFFRTERGIVLVTREMLEKLPATAATGKSRA"
     gene            2453..>3518
                     /locus_tag="PKX82_09330"
     CDS             2453..>3518
                     /locus_tag="PKX82_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOF52496.1"
                     /translation="MKILYVCTELFPLLKTGGLADVGAALPAALRSLGNEVRLLLPAF
                     PGIAQGVRIEGPALPLCTEGGPDILRTLQPAPRLLPGRVEATGQPVYLLDAPALYQRK
                     GGPYLDAHGREWTDNAERFALLCWAAAGLGLGEDPEWQPDVIHAHDWHTGLTPVYLRL
                     RARGMIAPACVFTVHNLAYQGVFPASVLPALGLPESLFTLDGIEFYGQLSFMKAGLQF
                     ADAITTVSPRYAREIMTPEQGCGLDGVLRYRADHVTGILNGVDYAVWDPSTDPLIDSH
                     FDARKMPGKSQAKCALQKSIGLEVRADAMLCVAVTRLSEQKGLHLVPAVLDDLVSRGG
                     QLLVLGSGDAVGSIKAVEARA"
BASE COUNT          650 a         1138 c         1052 g          678 t
ORIGIN      
        1 gacaggactg cactggccga gttcacccga ctacacgctg catggctgga cgactacgcc
       61 ctgttcatgg tgctggaaga ccgccatggc gcgccgtgga cccaatggcc tgcagcactg
      121 gcgaagcgcg ccccagaagc actgcatttg ctgcaaaagg aagaagcgca cgcgattggc
      181 ttctggcact ttgtccagtg gcgttttgat gtgcagtggc agcgtctgcg ccgcgatgcc
      241 catgcccgag gcattcacat cgtcggtgac gtgccgattt tcgtggccca ccacagtgcc
      301 gatgtctggg cgcacgccga ccagttcctg ctggatgggc acggtgatcc cctcgtcgtg
      361 gccggtgtgc caccggacta cttcagtgcc accggccagc gctggggcaa ccccctgtac
      421 cactgggata caatggcccg caacggctac cactggtgga aacagcggat gtctcatctg
      481 ttcagccagg tggacgtggc caggctcgac cactttcgcg gattcgaaac ctactgggaa
      541 attccggcca cagaatccac ggcccagggc ggccagtggc gcaacggtcc tggcaacgcc
      601 ttctttgccg ccttgcgcaa agagctgggt gccttgccca tcattgcgga ggacctgggc
      661 ctcatcaccg aagcggtgtc ccgattgcgc ctggactgcg gctttccggg catgcgggtc
      721 atgcaattcg cttttggcga cacggcccac aatccctacc tgccgcacaa tttcgaaccc
      781 ggcactgtgg cctataccgg aacccacgat aacgacacca ccatcggctg gtggcaacag
      841 gccatggagt ccgagcggca cgccgcacgc cagtacctgg gaccgcaaac cgaccaggaa
      901 atccactggg ccatgatcca gtccttgtca caatccgtcg cccgcaccct ggtgattcca
      961 ttccaggatg tgctgggtct ggatggtcgg caccggatga atacaccggg gcatccgcaa
     1021 ggatgctgga cctggcgatt ccactggtcc gaagtgaacg atctgcccgc agcgcgcctg
     1081 gccgccatgt cccgggcaca tgggcgcacg taatgccatc tgcttctgct tacccacacc
     1141 acccttgacg accacggaga gaacccatgg acaagaatgc accccatcaa ctcgcacgcc
     1201 gcaccattgc cctggtgctt gcaggtggcc ggggttcccg actgcatgca ctcaccgacc
     1261 ggcgcgccaa acccgcggtg tatttcggtg gaaagtttcg tatcgtcgat ttcgcgctgt
     1321 ccaattgcat gaactccggc gtacgccgca tcggcgtggt cacccagtac aagtcccaca
     1381 gcctgttgcg gcatctgcag cgcggctggt ctttcctgcg caacgagttc aatgaattta
     1441 tcgatctgct gcccgcccag caacgtctcg acgaatcatc ctggtaccag ggcaccgccg
     1501 atgcggtgtt tcagaacctc gacatcctgc gcgcccacaa gccagagtac atcctgatcc
     1561 tggccggcga ccatatctac aaaatggatt acgccaacat gattgccgac catgtggccc
     1621 agggcgcccg atgcactgtg gcctgcatcg aagttccgat caatgaggcc aaggtattcg
     1681 gggtcatgtc caccgacgca cagcggcgca tcgtgcgctt tgacgaaaaa ccgtcccagc
     1741 ccacccccat gccaggtcga cctgatatgg cgctggccag catgggcgtg tatgtatttg
     1801 acgccgacta cctgtacgcg gctctgaagg ccgatgccca gtccaccagt tcgagtcggg
     1861 attttggcaa agacctgatc cccgccatcg tggcacgcgg cgaagcggtg gcccatccct
     1921 ttggtcagtc ctgcatcaag agtagccccc aggctcctgc ctactggcgt gacgtaggca
     1981 cgattgacgc ctactgggcg gccaacctgg acctgacgaa caccattccc gaactggaca
     2041 tgtacgaccg cgattggccc atctggacct accaggagca actccccccg gccaaatttg
     2101 tgtttgatga tgacggacga cgcggcatgg tcgtggactc cctggtgtcg ggcgggtgca
     2161 tcatctccgg tgcatctgcg cgccgttcgg tgcttttttc caaggtgcgg ctgcattcct
     2221 acagcacggt ggaagaagcc gtgctgctgc cagaggtaga cgttgggcgc aactgccact
     2281 tgcgcaaggt cgtcattgac accggttgcc agattccggc gggcatgtcg attggctttg
     2341 atgctgctga agatgcgcgc cgcttctttc gtaccgagcg cggcattgtt cttgttacgc
     2401 gcgagatgct ggagaagctg cccgccactg cagcgacagg aaagtcccgc gcatgaaaat
     2461 tctgtatgtc tgcaccgagc tgtttccact gctcaaaaca gggggactcg ccgatgtggg
     2521 cgctgccttg ccagcggcct tgcgttcgct gggcaatgag gtccgcctgc tgttgccggc
     2581 gttccccggc attgcccaag gtgtccggat agaggggcca gccctgccac tgtgtaccga
     2641 gggcggacct gacatcctgc gcacactgca accggcgcca cggttgctgc cgggccgcgt
     2701 ggaagcgacc ggacagcccg tctacctgct cgatgccccg gcgctgtacc agcgcaaagg
     2761 cggcccctac ctcgacgcac acggccgcga atggaccgac aacgcggaac gctttgcgct
     2821 gctttgctgg gcggctgccg gactgggact cggggaagat ccggaatggc aacccgacgt
     2881 catccacgcc catgactggc ataccggtct cacaccggtt tacctgcggc tacgggccag
     2941 gggcatgatc gcgccggcct gtgtcttcac tgtccacaac ctggcctacc agggcgtgtt
     3001 cccggctagc gtgctacccg cactcggcct gccagagtcg ctgttcaccc tggatggcat
     3061 cgagttttat ggccagctct cgttcatgaa ggccggtctg caattcgccg atgccatcac
     3121 gaccgtcagc ccccgctatg cccgcgagat catgaccccg gagcagggct gtggcctgga
     3181 cggcgtgctg cgttaccgcg ccgaccatgt cacgggcatt ctgaatggcg tggattacgc
     3241 cgtctgggat ccctctaccg acccgctgat cgactcccac ttcgatgcca gaaagatgcc
     3301 cggcaagagc caggcgaaat gcgccctgca gaaatcgata gggctggagg tacgcgccga
     3361 tgcgatgttg tgcgtggcgg tcacccggct gagcgagcaa aaaggcctgc acctggttcc
     3421 agcggtgctg gatgatctgg tgtccagggg tgggcaattg ctggtgctgg gcagtggcga
     3481 tgcggtgggc tccatcaaag ccgttgaggc tcgcgcat
//
LOCUS       DAOSJM010000258         3518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23322_length_3518_cov_3.797863, whole genome shotgun sequence.
ACCESSION   DAOSJM010000258 DAOSJM010000000
VERSION     DAOSJM010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23322_length_3518_cov_3.797863"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PKX82_09335"
     CDS             complement(<1..374)
                     /locus_tag="PKX82_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOF52497.1"
                     /translation="MTLQATVSMSWVNTVLGAAERQGLEREPLLAAAGIPPADLALQR
                     WPIDHITRLWRTAAALTRDPGFGLKAGALVGPASFNVVSYILQSAPTLREAFGEVQQF
                     QRLISDGGRFQLIAGESASWIV"
     gene            486..2093
                     /locus_tag="PKX82_09340"
     CDS             486..2093
                     /locus_tag="PKX82_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52498.1"
                     /translation="MATQQQSDVLVIGGGLAGLVTTYECLQAGKRVTLVDRDSRERLG
                     GLALWAFGGMALVGTPLQARMKIPDTPELALRDWIRFGELGEDDVLPRQWARYYVEHS
                     RAQVYEWLMGKGIQFMPAVNWVERGMQGNGNSVPRYHIVWGTSRRLATRMIELMLQAG
                     EGGRLTLLSGHRVTALESSAGAVSGALAVNEATGQEVRLNAPVVVLAMGGINGSHEET
                     RANWPRHRPLPATLLNGAHPFADGKLHHLVAGLGGRITHAGEMWNYAAGFPHPFPHFE
                     GHGLSTIPCKSALWLNHRGERIGPEPLVTGFDTHELCQRVAAQEKPWTWHLLNWRIAA
                     KEFAISGAEHNQRIRDMQFPMFLKETLLGNHRLVKQMAAESRHFLVDDTLAGLAARMN
                     ALTASHDIDPAVLQATADRFDANFASGNKLHNDDQIRRIQHARQWGPDKLRTCKPAPL
                     QMPGAGPFIAIQMQLITRKSLGGLQTDLHSRVLDAAGQPLSGLYCVGEAAGFGGGGAS
                     GKRSLEGTFLPGCILTARTAARSIVGG"
     gene            2114..2977
                     /locus_tag="PKX82_09345"
     CDS             2114..2977
                     /locus_tag="PKX82_09345"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HOF52499.1"
                     /translation="MQGDIVSGVLLPLILGFIMFSLGLGLTPVDFRRILAQPRALLVG
                     VVCHFVLLPLVCYLMLKTLGITGVFAVGFMILAACPTGTTSNLLTYLARGDVALALSF
                     TAIASVLTIFTLPLLVTWSLGHFLGAARAVDVPVGMMMGQVAMVLGIPVSLGMFVRHR
                     WNDWALAFEPRATRIATGFFVLIVVLAVVKNWALLRDNFATLAPFALVLNITMLCIGF
                     AVAWAVRLSRAQSVTLGIETAVQNAALALVIASSVLKDDTLAIPGALYGVLMYAGGLA
                     FALAMRRFTRY"
     gene            2989..>3518
                     /locus_tag="PKX82_09350"
     CDS             2989..>3518
                     /locus_tag="PKX82_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOF52500.1"
                     /translation="MPNGAQALMKTLVDAGVEVCFTNPGTSEMHFVAALDSEPKMRAV
                     LALFEGVATGAADGYARMADKPAATLLHLGCGLGNGLANLHNARKGKVPVVNIVGDHA
                     TYHTQFDAQLQSDIETVARNVSPGFMRTSKITADLGRDTAEAVAAARGLPGQVATLIL
                     PADVSWGEGGVPSAPIA"
BASE COUNT          607 a         1085 c         1150 g          676 t
ORIGIN      
        1 tacacgatcc agctggcaga ttcgcccgca atcagctgga agcgcccgcc gtccgagatg
       61 aggcgctgga actgctgcac ctcgccgaac gcttcccgca gggtgggggc ggactgcagg
      121 atgtagctca ccacattgaa gcttgccggc cccaccagtg ccccggcctt gaggccaaag
      181 ccaggatccc tggtgagtgc ggcggcggtg cgccacagcc gggtgatgtg gtcgatgggc
      241 cagcgctgca gcgccagatc ggcgggcggt atgccggccg ctgccagcag gggctcgcgc
      301 tccagcccct ggcgctcggc tgcaccgagc accgtattca cccagctcat cgacactgtg
      361 gcctgtagag tcaatttgaa tggcctataa agtcaatcat gttcgcatca taagtcgcta
      421 tcgttggcat cgcacaaggc cgccgggcac agacaaagag cggccattac aacgaggaga
      481 cggacttggc aacacaacag caatccgatg tgctggtcat cggcggcggc ctggccggcc
      541 tggtgaccac gtacgagtgt ctgcaggctg gcaagcgcgt cacattggtg gaccgcgata
      601 gccgcgaacg cctgggtggc ctggccctgt gggcttttgg cggcatggcc ctggtgggca
      661 cgccactgca ggcgcgcatg aagattcccg acacgccgga gctggcgctg cgcgactgga
      721 tccgctttgg tgaactgggg gaggacgatg tgttaccccg ccagtgggcc cggtattacg
      781 tggagcactc gcgcgcgcag gtgtacgaat ggctcatggg caaaggcatc caatttatgc
      841 ccgccgtgaa ctgggtggag cggggcatgc agggcaacgg caattcggtg ccgcgctacc
      901 acatcgtgtg gggcacctcg cgccgcctgg ccacccgcat gatcgaactc atgctccagg
      961 caggagaggg cgggcggctg accttgctga gtggtcaccg ggtcaccgcg ctggagagca
     1021 gtgccggtgc ggtgagtggt gcgctcgcag tcaatgaagc caccggacag gaagtgcgcc
     1081 tgaacgcacc tgtggtggta ttggccatgg gcggtatcaa cggcagccac gaagaaacgc
     1141 gtgccaattg gcccaggcac cggcccctgc cggccaccct gctcaatggc gcgcacccgt
     1201 ttgcggacgg caagttgcac catctggttg caggacttgg agggagaatc acccatgcgg
     1261 gcgaaatgtg gaattacgcc gcaggttttc cccacccgtt cccgcatttc gagggacatg
     1321 ggctttccac cattccctgc aagtcggcgc tgtggctcaa ccaccgcggc gagcgcattg
     1381 ggcccgagcc gttggtgacg ggctttgaca cccatgagct ctgccagcgc gtggccgcgc
     1441 aggaaaaacc gtggacctgg catttgctca actggcgcat tgccgccaag gaatttgcca
     1501 tctccggggc cgagcacaac cagcgcatcc gcgacatgca gtttccgatg tttctcaagg
     1561 aaacgctgct gggcaaccat cgcctggtca agcaaatggc tgccgagagc cgccatttcc
     1621 tggtagatga caccctggcg ggacttgccg ccaggatgaa tgcgctgact gcctcgcacg
     1681 acatcgaccc cgctgtgctg caggccacgg cggaccgctt tgacgcgaat tttgcgagcg
     1741 gcaacaagct gcacaacgac gaccagatcc gccgcatcca gcatgcgcgg cagtggggcc
     1801 cggacaagct gcgcacctgt aagcccgcac cgctgcaaat gcccggtgcc ggaccgttca
     1861 ttgccattca gatgcagctc atcacccgaa aaagtctggg cggcttgcag actgatctgc
     1921 acagccgtgt gctcgacgcc gcgggccagc cgctgagtgg cctgtactgc gtgggtgagg
     1981 cggcaggttt tggtggtggc ggggccagtg gcaaacgctc actggaaggt acattcctgc
     2041 cgggctgcat actcactgcg cgcaccgcag cacgctccat cgtgggcggc taagaaaaaa
     2101 acgaggagat tgcatgcaag gtgacattgt ttcgggcgtg ctgctgccgc tcattctggg
     2161 tttcatcatg ttttcgctgg ggctggggct gacgcccgtg gactttcgtc gcatcctggc
     2221 gcagccgcgt gccctgctgg tgggggtggt gtgccacttt gtgctgctgc cgctggtgtg
     2281 ttacctcatg cttaagacac tgggcattac gggtgtgttt gccgtgggct tcatgattct
     2341 ggcagcgtgc cccacgggta ccacttccaa cttgctcacc tacctcgccc ggggtgatgt
     2401 ggcgctggcc ctgagcttta ccgccattgc cagtgtgctc accattttta ccctgccact
     2461 gctggtgacc tggtcgctgg ggcattttct gggcgcagcg cgtgcggtgg atgtgccagt
     2521 cggcatgatg atggggcagg tggccatggt gctggggata ccggtgagct tgggcatgtt
     2581 tgtgcgccac cgctggaacg actgggccct ggcctttgag ccccgtgcca cgcgcattgc
     2641 caccggcttc tttgtgctca tcgtggtgct ggccgtggtg aaaaactggg ccttgctgcg
     2701 tgacaacttt gccacgctgg ccccgtttgc gctggtgctc aacatcacca tgctgtgcat
     2761 cggttttgcc gtggcctggg ccgtgcgcct gtcgcgtgcc cagtcggtca cgctgggcat
     2821 agaaaccgcc gtgcaaaacg ccgcgctggc cctggtgatt gccagttccg tgctcaagga
     2881 cgataccctg gccattcccg gtgcactgta tggggtgctt atgtacgcag gtggcctggc
     2941 gtttgcactc gccatgcggc gttttacccg ttactgaagg agagttccat gcccaacggc
     3001 gcacaagccc tgatgaagac cctggtcgat gccggtgtcg aagtctgctt taccaacccc
     3061 ggaaccagcg aaatgcactt tgtggcggcg ctcgacagcg agcccaagat gcgcgcagtg
     3121 ctggccctgt ttgaaggcgt cgccacgggt gctgccgacg gctacgcccg tatggccgac
     3181 aagcctgctg caaccctgct gcatttgggc tgcggcctgg gcaacggcct ggcgaacttg
     3241 cacaatgccc gcaaaggcaa ggtgccggtg gtcaacatcg tgggcgacca cgccacctat
     3301 cacacgcaat tcgacgccca gttgcagtcc gacattgaga ctgtggcgcg caatgtgtcg
     3361 ccagggttta tgcgcacctc caaaatcacg gccgatctgg gacgcgacac cgccgaggct
     3421 gtggcggctg ccagaggcct gccgggtcag gtggccacgc tcatcctgcc ggccgatgtg
     3481 tcctggggcg agggcggcgt gccgtctgca ccgattgc
//
LOCUS       DAOSJM010000259         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23384_length_3510_cov_4.628944, whole genome shotgun sequence.
ACCESSION   DAOSJM010000259 DAOSJM010000000
VERSION     DAOSJM010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23384_length_3510_cov_4.628944"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PKX82_09355"
     CDS             <1..358
                     /locus_tag="PKX82_09355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOF52501.1"
                     /translation="GLAAFLGHLYPVFFRFVGGKGVATALGVLVGLSGWLGLAVMATW
                     LLVAFFSRYSSLASLLAAICAPVYYLLGDGTAWYMDARVLVSVVVMSALLIYRHAENI
                     SRLIKGTESRLGKKKT"
     gene            complement(362..1318)
                     /locus_tag="PKX82_09360"
     CDS             complement(362..1318)
                     /locus_tag="PKX82_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOF52502.1"
                     /translation="MTTLRVRDENEYEDLLAEWSDLESGLGMILGNPESVQQFAHRVL
                     QYDRWMQGLLQRDPDVGLYLLFQLAGNSPVGYSASHALVCAVLCHLIASELLLPPRER
                     DSLVRAALTMNIAMTRVQDELATQVEKPSPPQQEVIRTHPVKGAMMLGTLGVQDENWL
                     DVVSRHHDDSEPGVDLRAMSAGRRLTHILHVVDRYAAMISPRVSRAGRSATDSARTIL
                     SNRTDPVGQALVRSVGLCPPGTYVRLDNDELGVVLRRSSKANQPYVAIVGTASGEVFA
                     APRLHGTAQGYPRIKAALSTTAVREPLNHYVILRLNAHPAQG"
     gene            complement(1326..1973)
                     /locus_tag="PKX82_09365"
     CDS             complement(1326..1973)
                     /locus_tag="PKX82_09365"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HOF52503.1"
                     /translation="MSTRHLLSLLALHCVFGAAVAQTVGLAGMLGGKALVIVDGSPPK
                     TVAPGETFAGVKIVSTQGDQAVVEIAGKRHTLRVGDAPASVGGGGGDAGGNRVVLPAG
                     SGGHFFTQGQINGRTVQLVVDTGASVVSLSTADAQRIGLNYKNGQPAQLSTANGVIPA
                     WRVKLGSVRVGDVVVYDVDAVVSSGAMPYVLLGNSFLSRFQMTRTNDQMVLDKRF"
     gene            complement(1977..2462)
                     /locus_tag="PKX82_09370"
     CDS             complement(1977..2462)
                     /locus_tag="PKX82_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOF52504.1"
                     /translation="MPSFDTVCEANLVKVKNGVENTTKEITTRFDFKGTSAKIEIKDK
                     EITLIGDAEFQLDQIEDVLRNKLTKQEVDVRFLDKGDVQKMGGGKVKVVIKVRNGIEA
                     ELSKKIQRLLKDSKLKVQASIQEEKVRVTGAKRDDLQAAMALIRKEVTDIPLSFDNFR
                     D"
     gene            2498..>3510
                     /gene="murB"
                     /locus_tag="PKX82_09375"
     CDS             2498..>3510
                     /gene="murB"
                     /locus_tag="PKX82_09375"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOF52505.1"
                     /translation="MLVEKNVPLQALNTFRIVAKAHTLVRIKDPSDIAAVLADPEIGP
                     APKFVLGGGSNIVLTGDVKPVVVKVEVKGMRLVQETAKAWILEAGAGENWHDFVAWTL
                     DQGYPGLENMALIPGTVGASPVQNIGAYGVELQDRFESLDAVDLQTGQAFTLNAAQCA
                     FGYRDSVFKHGGPTSMGLKDRAVIVRVRFALPKVWKPVLGYADIEKKMTEQACAHPTA
                     RQLFDWVCAIRRAKLPDPDVIGNAGSFFKNPTVTPEQCADIIARDPKVVHYRMDDGSI
                     KLAAGWLIDSCGWKGKSVGQAGVYEKQALVLVNRGAGSDGQGATGGEVMTLAKAIQTS
                     VYER"
BASE COUNT          620 a         1062 c         1110 g          718 t
ORIGIN      
        1 tggactggcg gctttcctgg ggcatctgta cccggtgttc ttccgctttg tgggcggcaa
       61 gggcgtggcc acggcgctgg gcgtgctggt gggactcagc ggctggctgg ggctggccgt
      121 catggcaacc tggctgctgg tggcgttctt ctcccgctat tcgtcgctgg cttcgctgct
      181 ggcggccatc tgcgccccgg tgtactacct gctgggtgac ggcaccgcgt ggtacatgga
      241 tgcgcgcgtc ctcgtgagtg tggtggtgat gtcggctttg ctgatctacc ggcatgcgga
      301 aaatatttcc cgcctgatca agggcacgga atcgcgcctg ggcaagaaaa agacctgacg
      361 ctcagccctg tgccgggtgc gcgttgagcc gcaatatcac gtagtggttg agtggttcgc
      421 gcaccgccgt ggtagacagt gcagccttga tgcgcgggta accctgggcg gttccatgca
      481 accggggcgc cgcaaacact tcgcctgacg ccgtgccgac gatggcgacg tagggctggt
      541 tggctttgct gctgcgccgc aggaccacgc ccaactcatc gttgtcgagt cgcacatagg
      601 tgcctggcgg acataagcca accgagcgca ccagcgcctg gccgacaggg tcggtccggt
      661 tcgacaggat ggtgcgtgcc gaatcggtgg cgctgcggcc cgcacgcgat acccggggac
      721 tgatcatggc ggcatagcgg tccaccacat gcaggatgtg tgtcaggcgc cgaccagccg
      781 acatggcgcg caggtccacg ccaggttccg agtcgtcatg gtgcctggag accacatcca
      841 gccagttttc gtcctgcact cccagcgtgc ccagcatcat cgcgcccttg acgggatggg
      901 tgcggatcac ctcttgctgt ggtggagagg gtttttccac ctgggttgcc agttcgtcct
      961 ggacgcgggt catggcgatg ttcatggtca gtgcggcccg caccaggctg tcacgctcgc
     1021 gcggtggaag cagcagttcg gaggcaatca ggtggcacag cacggcgcag accagtgcgt
     1081 gcgatgcgct gtaacccacc ggcgaattgc cggccagctg gaacagcagg tacaggccga
     1141 cgtccgggtc gcgctgcagc aggccttgca tccagcggtc gtattgcagc acgcggtggg
     1201 caaattgctg gacactttcg gggttaccca gaatcatgcc cagacccgac tccagatcgg
     1261 accactcggc cagcaggtct tcgtactcgt tttcgtcccg aacgcgcagg gtggtcatgg
     1321 caaattcaga atcgcttgtc cagcaccatc tggtcattgg tgcgggtcat ctggaagcgg
     1381 gacaggaaac tgttgcccag cagtacatac ggcatggcgc ctgaggacac cacggcgtcc
     1441 acgtcataca ccacgacatc gcccacccgc accgacccga gcttgacccg ccaggcggga
     1501 atcacgccat tggcggtgct cagttgggcc ggctgcccgt ttttgtaatt cagtccgatg
     1561 cgctgggcat cggctgtgct gagcgatacc accgatgccc cggtgtcgac caccagttgc
     1621 accgtccgtc cgttgatctg tccctgggtg aaaaaatgcc cgccactgcc agcgggcagc
     1681 accacccggt tgccaccggc atcgccaccg ccgccaccca cactggctgg ggcgtcgccc
     1741 acgcgcagcg tatggcgctt gcctgcaatc tccacgaccg cctggtcgcc ctgggtggac
     1801 acgatcttca cgccggcgaa cgtttccccc ggggccacgg tcttgggcgg gctgccgtcc
     1861 acaatcacca gtgccttgcc cccgagcatg cctgccagtc ccacggtctg cgccaccgct
     1921 gcaccgaata cacagtgcaa ggcaagaagg gatagcaggt ggcgtgtgga catcaatcag
     1981 tcgcggaagt tgtcgaaact cagggggatg tcggtcactt ccttgcggat gagggccatc
     2041 gcggcctgca ggtcgtcgcg cttggcgccg gtcacgcgta ctttctcttc ctggatggac
     2101 gcctgtacct tgagtttgct gtccttgagc aggcgctgga ttttcttgga cagttcggcc
     2161 tcgatgccat tgcgcacctt gatcacgacc ttaaccttgc cgccacccat tttctgcacg
     2221 tcgcctttgt ccagaaagcg cacatcgact tcctgtttgg tcagcttgtt gcgcagcacg
     2281 tcctcaatct ggtcgagctg gaactcggca tcaccgatca gggtgatttc cttgtccttg
     2341 atttcaatct tggcggatgt tcccttgaag tcaaagcggg tggtgatttc cttggtggtg
     2401 ttttccaccc cgtttttcac cttgaccagg ttggcttcgc agacggtatc aaatgaaggc
     2461 atacaggtat tcaaataggc agtgagacaa tcctgccatg ttagtcgaga aaaacgtccc
     2521 gcttcaagcc ctcaatacct tccgcatcgt tgccaaagca catactttgg tacggatcaa
     2581 ggatccatcg gatattgcag ccgtgctggc cgatcccgaa atcgggccgg cgcccaaatt
     2641 cgtgctgggt ggtggcagca acatcgtgct gaccggcgat gtcaaacctg tggtggtcaa
     2701 agtcgaggtc aagggcatgc ggctggtgca ggaaactgcc aaagcctgga ttctggaagc
     2761 tggcgccggc gagaactggc atgactttgt ggcctggacg ctggaccagg gctacccggg
     2821 tctggagaac atggccttga ttccgggcac agtcggagcc tcgccggtgc agaacatcgg
     2881 tgcctacggc gtcgagttgc aggaccggtt tgaatcgctg gatgcggtgg atctgcagac
     2941 cggtcaggcc tttaccctca atgccgcgca gtgtgccttt ggctaccgtg attcggtgtt
     3001 caagcatggt ggccccacgt ccatgggcct gaaagaccgc gccgtgatcg tgcgggtgcg
     3061 ttttgccttg cccaaagtct ggaagccggt gctgggctat gccgatatcg aaaagaaaat
     3121 gaccgagcag gcctgcgccc atccgaccgc gcggcagctg ttcgactggg tctgcgccat
     3181 ccgccgcgcc aaattgcccg atccggatgt gattgggaat gcaggcagtt ttttcaagaa
     3241 ccccacggtg acgcccgagc agtgtgccga catcattgcc cgcgacccca aggtcgtgca
     3301 ctaccgcatg gacgatggct ccatcaaact ggccgctggc tggctgattg attcttgtgg
     3361 ctggaaaggc aagtcggtag ggcaggccgg cgtatatgaa aagcaggcgc tggtgctggt
     3421 caaccgcggc gccggctcgg acggccaggg cgcgaccggt ggcgaggtca tgacgctggc
     3481 aaaggccatc cagaccagcg tgtacgaacg
//
LOCUS       DAOSJM010000260         3506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23407_length_3506_cov_5.445958, whole genome shotgun sequence.
ACCESSION   DAOSJM010000260 DAOSJM010000000
VERSION     DAOSJM010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23407_length_3506_cov_5.445958"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1892)
                     /gene="secD"
                     /locus_tag="PKX82_09380"
     CDS             complement(12..1892)
                     /gene="secD"
                     /locus_tag="PKX82_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOF52506.1"
                     /translation="MNRYPVWKYAIILVALLVSGLYALPNVFGEAPAVQVSAAKATMK
                     LDASALARVEDALKAAGIAADAVTLDGTSIKARFSNTDAQLKAKDAIQKALNPDAADA
                     GYVVALNLLSRSPAWLTALHASPMYLGLDLRGGVHFMLQVDMQAALSKKAESLAGDIR
                     SVLREKNVRHSGISRNGQTIEIRFRDTQALEAARRVVADQFGDLTTVESPDGTEFKMV
                     ASIKPVAAKSVQDQALKQNITTLHNRINELGVAEPVIQQQGQDRIVVQLPGVQDTAKA
                     KDILGRTATLEIRMVEESAEARAAETGTGPVPFGSERYLERNGQAVIVKKQVILTGDN
                     LTDAQPGFDNQTQEAAVHLTLDAKGARIFRDVTRESIGKRMAILLFEKGKGEVVTAPV
                     IRSEIGGGRVQISGRMTTVEAADTALLLRAGSLAAPMEIIEERTIGPSLGAENIAKGF
                     NSVTWGFVAVAIFMCAYYMLFGVISSIALAFNLLLLVAVLSMLQATLTLPGMAAMALV
                     LGMAIDANVLINERVREELRNGASPQAAIHAGYDRAWATIIDSNVTTLIAGLALLAFG
                     SGPVRGFAVVHCLGILTSMFSGVFFSRGVVNLWYGRQKKLKSVSIGTVWRPDSSNQVA
                     TN"
     gene            complement(1911..2243)
                     /gene="yajC"
                     /locus_tag="PKX82_09385"
     CDS             complement(1911..2243)
                     /gene="yajC"
                     /locus_tag="PKX82_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOF52507.1"
                     /translation="MFISSAFAQTAPAAAAGGDMQSSLMSMLPLVLMFVVLYFVMIRP
                     QMKKQKEHKAMIDALAKGDEVVTAGGVLGKVSKMGDIYVGLEVATGVEVQVQRSAVVQ
                     VLPKGSIK"
     gene            complement(2381..>3506)
                     /locus_tag="PKX82_09390"
     CDS             complement(2381..>3506)
                     /locus_tag="PKX82_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOF52508.1"
                     /translation="MASADLILGCDPIVVAGKETLLRMRPGRTHVALNAVSTPTAAFV
                     KNANWVNPSEQCLVDIAHAVGAEHVGAFDADAVAVKVLGDSIYTNPMMLGFAWQKGWI
                     PLQLESLMRAIELNAVAVAQNKLAFEWGRRAAQDVDAVKRALNPGQVIAFSPRQKQSL
                     DDLVAHRVAFLREYQNAAYAQQYLDFVSKVRAAEQGMGVAEKLPLTEAVARYLFKLMA
                     YKDEYEVARLYTDPAFQAKVNAMFEGDFKLHYHLAPPMLARTNDKGELQKTSFGPWMR
                     LGFRLLAPMKVLRGSALDVFGKTEERRQERALIQEYRAAIEGMLPLLSPANRDVAAAF
                     ARVPEQIRGFGHVKARHLVAARTQWDLLQTQFHAPKQQAA"
BASE COUNT          746 a         1135 c          957 g          668 t
ORIGIN      
        1 gccttttccc tttagtttgt cgctacctga ttactggaat cggggcgcca aaccgtaccg
       61 atggatacgc tcttgagctt tttttgtcga ccgtaccaca aatttaccac gccacgggag
      121 aagaacacgc ccgagaacat actggtcagg atgcccagac aatgcaccac ggcgaaccca
      181 cgtaccggac cagaaccaaa agccagcagt gccagacccg cgatcagcgt ggtcacattg
      241 gagtcgatga tggtcgccca ggcgcggtca tatccagcat ggatagccgc ctggggcgat
      301 gcaccattgc gcaactcctc acgcacccgt tcgttgatca gcacgttggc atcaatcgcc
      361 ataccaagaa ccagcgccat ggccgccatg ccaggcagtg tcaacgtggc ctgcagcata
      421 gaaagcacag ccaccagcag caacaggttg aatgccagcg caatactgga aatcacgcca
      481 aacagcatgt aatacgcaca catgaagatg gccaccgcca caaaacccca agtcacgctg
      541 ttgaagccct tggcaatgtt ctcggcaccc aggctgggac caatggtgcg ctcttcgatg
      601 atttccatgg gcgcggccag tgagccggcg cgcagcaaca gggccgtatc cgcagcctct
      661 accgtagtca tgcggccgga gatctgcacc cggccacctc caatttcaga gcgaatcact
      721 ggcgcagtca ccacttcgcc cttgcccttt tcaaagagca gaatcgccat gcgcttgccg
      781 atgctctcgc gggtcacatc gcgaaagatg cgtgcgccct tggcatcgag cgtcaggtgc
      841 actgcggctt cctgagtctg attgtcaaag ccaggttgcg catcggtcag gttgtcaccg
      901 gtcaggatca cctgtttctt gacgatcaca gcctggccat tgcgctccag atagcgctcg
      961 gagccaaaag gcacaggacc ggtaccggtc tccgcggcgc gggcctcggc actctcttcc
     1021 accatgcgga tttccagcgt ggccgtgcgt cccagaatat ccttggcttt ggccgtatcc
     1081 tgtacacccg gcaactgcac cacgatgcga tcctggccct gctgctggat cacaggctcg
     1141 gccacaccca attcattgat gcgattgtgc aaggtggtga tgttctgctt gagcgcctgg
     1201 tcctgaacgg acttggccgc cacaggcttg atgctggcta ccatcttgaa ctcggtgcca
     1261 tcgggagact ccaccgtggt cagatcacca aactggtcag ccaccacgcg ccgcgccgct
     1321 tccagcgcct gtgtatcgcg gaaccggatt tcaatggtct ggccattgcg tgaaatgccg
     1381 ctatgccgga cattcttttc ccgcaacacg gagcggatgt cgcccgccag tgactcggcc
     1441 ttcttgctca gcgccgcctg catgtccacc tgcagcatga agtgcacccc accgcgcaaa
     1501 tccagcccca ggtacatggg cgaagcatgc agagcggtca gccaggcggg agagcgcgac
     1561 aggaggttga gtgccaccac atagccagca tccgcagcat caggattcaa cgccttctgg
     1621 atggcgtcct tggctttgag ctgcgcatcc gtattggaaa aacgcgcctt gatggacgtg
     1681 ccatcgagag tcaccgcatc cgcggcaata cccgctgcct tgagcgcatc ctctacccgc
     1741 gccagtgcgg atgcatccag cttcatggtc gccttggcag cggacacctg caccgccggg
     1801 gcctcgccaa acacattggg cagggcatac agaccactga ccagaagcgc gaccagaatg
     1861 atcgcgtatt tccacaccgg gtaacggttc atgatgaaaa caggatgggg ttacttgatg
     1921 ctgcccttgg gcaaaacttg cacgactgcg ctgcgctgga cttgcacttc aacgcctgta
     1981 gccacttcca gaccgacata gatatcgccc attttgctca ccttgccgag cacgccgcca
     2041 gcggtcacca cctcatcgcc cttggccagg gcgtcgatca tggctttgtg ttccttttgc
     2101 ttcttcatct ggggacgaat catcacaaag tacagcacca caaacatcag gaccaggggc
     2161 aacatgctca tcagggagga ttgcatatca ccaccagcgg cagcagcggg agcggtttgg
     2221 gcgaaagcgg aagaaataaa cactggacac tccacaaatg ggtcagaaat aaaacacttg
     2281 tccccgtggc agcggcgcgc accaacgcgc cgcaccgagg cggacagcct gacattgtaa
     2341 ggggcgggcc tgcaaaagcc caacccgcac aggcaaggcc ttatgcagcc tgttgtttag
     2401 gcgcgtggaa ttgcgtctgc agcaggtccc actgcgtgcg cgcagcgacc agatggcgcg
     2461 ccttgacatg gccaaagccc cgaatctgct cgggcacccg ggcaaaagcc gccgcgacat
     2521 cccggtttgc cggactgagc agcggcagca tgccctcaat ggcagcgcgg tattcctgaa
     2581 tcagtgcgcg ctcctggcga cgctcctcgg tcttgccaaa gacatccagc gcacttcccc
     2641 gcaatacctt catgggtgcg agcagcctga atcccagtcg catccagggt ccaaagctgg
     2701 ttttctgaag ctcgcccttg tcgttggtgc gtgcaagcat aggtggcgcc aggtggtaat
     2761 gcagcttgaa gtcgccttca aacatggcat tgaccttggc ctggaacgcc gggtccgtat
     2821 acagacgggc cacttcatat tcatccttgt aagccataag cttgaacaga tagcgcgcta
     2881 ccgcctcggt cagcggcaac ttttcagcga cgcccatccc ttgctctgcc gcacgcacct
     2941 tgctcacaaa gtccagatac tgctgcgcgt acgccgcatt ctggtattcc cgcaaaaacg
     3001 ccacccggtg cgctaccaag tcgtccaacg actgcttctg ccgcgggctg aacgcaatga
     3061 cctgtcccgg attgagcgcg cgcttcacgg catccacgtc ctgggccgca cgccgccccc
     3121 actcaaacgc cagcttgttc tgcgccacgg ccaccgcgtt gagttcgatg gcgcgcatca
     3181 gggattccag ctgcaacgga atccagcctt tttgccaggc aaaacccagc atcatggggt
     3241 tggtgtaaat gctgtcaccc agcaccttca cggccacggc atcggcgtca aaagcaccca
     3301 catgctcggc acccaccgcg tgcgcaatgt cgaccagaca ctgctcgctg gggttgaccc
     3361 aattggcatt cttcacaaag gccgcagtgg gtgtactcac ggcgttgagc gccacatggg
     3421 tgcgaccggg gcgcatgcgc agcagggttt ccttgcctgc caccacgatc gggtcgcagc
     3481 ccaagatcag atcggctgag gccatg
//
LOCUS       DAOSJM010000261         3483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23584_length_3483_cov_4.662777, whole genome shotgun sequence.
ACCESSION   DAOSJM010000261 DAOSJM010000000
VERSION     DAOSJM010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23584_length_3483_cov_4.662777"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="PKX82_09395"
     CDS             <1..759
                     /locus_tag="PKX82_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOF52509.1"
                     /translation="RVADAALMMQVLSRPDARDSMSLPPAQVAWDDWEGAIDHLRGLR
                     IGLLIDAGCGLAVDPEVRDAVLHAATLLDDAGAIVEPMEPFFTRQMLDGMDHAWRMRS
                     HLDLAALPAHRRDTVLPYIHTWADSVKDMSGTAAFVAMSQFHATRVATVRACNPYDFV
                     ISPTAPNVAFAAELPSPTNDPLRPLEHIAFTVPFNMSEQPAASINCGYTEAGLPIGLQ
                     IAGRRFDDLGVLRISRAFELLRDAQRPWPEPPNA"
     gene            complement(893..1342)
                     /locus_tag="PKX82_09400"
     CDS             complement(893..1342)
                     /locus_tag="PKX82_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOF52510.1"
                     /translation="MKILLPVDGSELSLEAVRFAVRLLKNGLQGTVVLANVQEPATLY
                     ELIVAHDPEVIDRVSAEAGLHTLASAQALLDAAAVGYECEVAKGDPAHTIIDIAERFA
                     CDMVVMGARGTSALRSAMMGSVSNEVLHASSVPVVIVKAAEEASSSD"
     gene            complement(1358..1846)
                     /gene="coaD"
                     /locus_tag="PKX82_09405"
     CDS             complement(1358..1846)
                     /gene="coaD"
                     /locus_tag="PKX82_09405"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOF52511.1"
                     /translation="MSNPTIAVFPGTFDPITLGHQDLIRRSASLFGTVIVAVAVAHHK
                     KTMFTLDERLAMVREVFRALPNVQVESFTGLVRDFAVNHGAQVMVRGVRSVTDFDYEA
                     QLAGMNRALAPDVDTVFLTPDARYQYISSTLVREIATLKGDVAQFVAPGVHAQLMVKL
                     RS"
     gene            complement(1878..2525)
                     /gene="rsmD"
                     /locus_tag="PKX82_09410"
     CDS             complement(1878..2525)
                     /gene="rsmD"
                     /locus_tag="PKX82_09410"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOF52512.1"
                     /translation="MKAKVSPSGAAGKRPVRPRGIQSHEIRIIGGQWKRTKLKVADKP
                     GLRPTPDRVRETLFNWLGQDLSGQRCLDVFAGTGALGFEAASRGAAEVVLVEQDAALV
                     AQLQQVRTQLLALQPLEPVAPPVLLVQRGEGVSALRQAAPASRDLIFLDPPFDAPLFE
                     PALAACARALTPRGLVYLEAPVQWTDAQLAPLGLAVQRHLKAGAVHAHLLRLAVA"
     gene            2640..3338
                     /locus_tag="PKX82_09415"
     CDS             2640..3338
                     /locus_tag="PKX82_09415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF52513.1"
                     /translation="MRGLAVFLWFELAASAVALLAAVLNAYVGVALLVVVVASLAAKI
                     LRLQRQQGSLQREIATLVRNEQRLKSQAYTDPLTGLANRLLLTDRFRQTAERNKRNRT
                     EFAVLMVDLNSFKVINDTLGHAAGDHVLKTVGRRLVAAVRASDTVARLGGDEFVLIVE
                     SFNHPDELLPIGRKLIDAISEGIALPAGEVVNVGASVGFALYPHHGTDINDLLHVADK
                     GMYDCKISGLMELH"
BASE COUNT          644 a         1176 c         1055 g          608 t
ORIGIN      
        1 cgcgtggccg atgctgcgct gatgatgcag gtgctaagcc gccccgatgc ccgcgacagc
       61 atgagcctgc caccggcgca ggtggcctgg gacgactggg aaggcgccat cgaccacctg
      121 cggggcctgc gcattggctt gctgatcgac gcgggctgcg gcctggccgt ggaccctgaa
      181 gtacgcgacg ccgtgctgca tgcggccacg ctgctcgacg acgcaggcgc gatcgtcgaa
      241 cccatggagc ccttcttcac acgccagatg ctcgacggca tggaccacgc ctggcgcatg
      301 cgttcgcacc tcgacctggc ggccttgccc gcgcaccggc gcgacacggt gctgccctac
      361 atccacacct gggccgacag tgtgaaagac atgtcaggca ccgcagcctt tgtcgccatg
      421 agccagttcc acgccacacg cgtggccacg gtcagggcgt gcaaccccta tgacttcgtg
      481 atctcgccca cagcaccgaa cgtggcattc gcggccgaac tgccctcgcc caccaacgat
      541 ccgctgcgcc cgctggagca cattgcgttc accgtgccct tcaacatgtc ggaacagcct
      601 gcggccagca tcaactgcgg ttacacggaa gctggcctgc ccatcggcct gcaaattgcc
      661 gggcgccggt ttgacgatct gggcgtgctg cggatcagcc gcgcctttga gttgctgcgc
      721 gatgcacagc ggccatggcc tgagccgccg aatgcatgaa gacacttcgg gactcagggc
      781 ggcgcagcac tcagctccgc ggctgtcccc cgcttcgctc ctcctttatg ccgctacgct
      841 gaatgccagc gtcgtcctga gtcacacagt ctctattggc atacgcaggg tttcagtcac
      901 tgctgctggc ttcttccgcg gccttgacga tgaccaccgg cacgctgctg gcgtgcagca
      961 cttcattgga aaccgagccc atcatcgcgc tgcgcagggc gctggtaccg cgggccccca
     1021 tcaccaccat gtcgcacgca aagcgctctg caatgtcaat gatggtgtgc gccggatccc
     1081 cctttgccac ttcacactcg tagcccaccg cggcagcgtc aagcagggcc tgcgcgcttg
     1141 ccagcgtatg caagcccgct tctgcactca cccggtcgat gacctcggga tcgtgggcca
     1201 cgatcagctc atacagtgtg gcgggctcct gcacattggc cagcaccacc gtgccttgca
     1261 gaccattttt gagcaggcgc acggcaaagc gcaccgcctc cagagacagt tccgaaccat
     1321 cgaccggcaa taaaattttc atggtgttct cctgggttca gctccgcaat ttgaccatca
     1381 gctgcgcatg cactccgggc gccacgaatt gcgctacatc gcccttgagc gttgcaatct
     1441 cgcgcaccag ggtactcgag atgtactggt agcgcgcatc gggcgtgagg aaaacggtgt
     1501 ccacatccgg ggccagcgca cggttcatgc cggccagctg cgcttcgtaa tcgaaatccg
     1561 tcacagagcg cacaccgcgc accatcacct gggcaccgtg gttgaccgca aaatcccgca
     1621 ccagtccggt gaagctttca acctgcacat tgggcagcgc acgaaagact tcgcgcacca
     1681 tcgccagccg ctcatccagt gtgaacatcg tcttcttgtg gtgcgccacc gccaccgcga
     1741 cgatgacggt gccaaacagg ctggcactgc ggcggatcag atcctggtgc cccagggtga
     1801 tggggtcaaa cgtgccagga aacacggcga tggtggggtt ggacatacca ggctccggcg
     1861 gaaagaagtg ttacaaatta tgcgacggcc aggcgcagca aatgggcatg caccgcaccg
     1921 gccttgaggt ggcgctgcac cgccagcccc aggggggcaa gctgcgcatc ggtccattgc
     1981 accggcgctt ctaggtacac cagcccccgc ggcgtcaggg cccgcgcaca ggctgccagt
     2041 gccggctcaa acagcggcgc atcgaatggt gggtccagaa agatcagatc gcggctggct
     2101 ggtgctgcct gccggagggc agaaacacct tcaccgcgct gcaccagcag aaccggtggc
     2161 gctaccggct ccagcggctg cagtgccagc agctgggtgc gcacctgctg caactgggct
     2221 accagcgcgg catcctgctc gaccagcacc acttcggcgg cgccgcgtga agcggcctca
     2281 aaacccagcg cccccgtgcc agcaaagaca tccaggcaac gctggccgct gaggtcctgc
     2341 cccagccagt tgaacagcgt ctcgcgcacc cggtccggcg taggccgcag accgggcttg
     2401 tcagccacct tgagtttggt gcgtttccat tggccgccga tgatgcggat ttcgtgggac
     2461 tggatgccgc gcgggcgtac gggacgtttg ccggcagcgc cggagggaga aactttggct
     2521 ttcatgggta cagcttaacc caaggacagg gtgcatatct cgtaacaaat tcacccgatt
     2581 gggggatgga cacaccaagc acctgcaacc atgattcgct atctgcaaca aagggttcca
     2641 tgcgcggtct ggcggttttc ctgtggtttg aactggctgc cagtgcggtg gccctgctgg
     2701 ctgctgtcct caacgcctat gtcggcgtcg cattgctggt ggtggttgtc gccagcctgg
     2761 ccgccaaaat cctgcgcctg cagcgccagc aagggtctct gcagcgggag attgccacgc
     2821 tggtgcgcaa cgagcagcgc ctcaagagtc aggcatacac cgatccgctc acgggcctgg
     2881 ccaaccgcct gctgctcacc gaccggttcc ggcagacagc cgagcgcaac aagcgcaacc
     2941 gcaccgaatt tgccgtgctg atggtggacc tgaacagctt caaggtcatc aacgacacgc
     3001 tgggccatgc cgcaggcgac cacgtactca aaaccgttgg cagacgcctg gtggcggcgg
     3061 tgcgcgcctc cgatacggtg gcccgactgg ggggtgatga atttgtgctg atcgtcgaat
     3121 cgtttaacca cccggatgaa ctgctcccca tcgggcgcaa gctgatagac gccatctcgg
     3181 aaggcattgc attgccggcg ggcgaggtcg tcaatgtggg tgccagcgtg ggctttgcgc
     3241 tgtaccccca ccatggcacc gatatcaacg acctgctgca cgtcgccgac aagggcatgt
     3301 acgactgcaa gatctccggg ctgatggagc tgcactaagc ccctgcggca cgcacggtgc
     3361 tcagggcgcc cccagcacca cggcggccat cgtctgcggc tgcaccacgc gcgcaaacgc
     3421 attgcgcaca tccttcaccg tcagcttcgc cacctgcgcc gcccaggtgt ccaggtaatc
     3481 cag
//
LOCUS       DAOSJM010000262         3479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23617_length_3479_cov_4.748832, whole genome shotgun sequence.
ACCESSION   DAOSJM010000262 DAOSJM010000000
VERSION     DAOSJM010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23617_length_3479_cov_4.748832"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKX82_09420"
     CDS             <1..418
                     /locus_tag="PKX82_09420"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HOF52514.1"
                     /translation="YNMSDYFQHWLDMEHKLEESGHTLPKIYCVNWFRKGDDGKFVWP
                     GYGDNMRVLKWMIDRLEGTAQGTATGFGVAPQYSEINWTGLDFSQAQFDSVTSLDKAA
                     WKQEFALHSELFTQLAYHLPKELEETKAKLEASLTA"
     gene            complement(479..934)
                     /locus_tag="PKX82_09425"
     CDS             complement(479..934)
                     /locus_tag="PKX82_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52515.1"
                     /translation="MARFTQPLFASQAGAESLGAPSATYGGQSKDTEGSRTLAGMLLA
                     AVMAALLVVADQVIDTWADGHLLAGWVALWTVAFAALALLAPPLRQGTSAMAALYARW
                     ARAAALRRQEEAMWDYAQRDPRVMAELQMAWMREHDSALPFDPLRAASR"
     gene            1064..2026
                     /locus_tag="PKX82_09430"
     CDS             1064..2026
                     /locus_tag="PKX82_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF52516.1"
                     /translation="MPTLNLRTFDLNLLKVFDAVMAERSLTRAAHQLSLTQPAVSNAL
                     RRLREALGDDLLMRKGRGLEPTVRALELWPAVQEALQKLQTSLAPSVFDPATATATTS
                     FVLTMADATAAELMPGLVDVITREAPGVTLRVVPLTTRDPRRLLDEGQADLAIGHFPA
                     VLADLTARAQSGEAVSFLHHRLFAGDYVCVMRKNHPLAQGPLTLDQYCAARHMLVSFS
                     GRAFGFIDEALALLHRKRPVVLTVNQFFTAGKVVAHSDLLTVLPRHFVNVTGFVDQLE
                     QRELPFQVPPIQVDALWHQRQNGSSAHAWLRAQVVALADKAFPA"
     gene            2034..2855
                     /locus_tag="PKX82_09435"
     CDS             2034..2855
                     /locus_tag="PKX82_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOF52517.1"
                     /translation="MKIQLLSDLHLEAHPHWRATPAAGADVLVLAGDVGSYQPGSLLT
                     DSDFGLGQFSAASGWPTPVIFVPGNHEYDGMDFDAAHERLRATCARLGLHWLERETLT
                     LHGVRFVGTTLWTDFDALAPNADHSTTALAQQLKARDKAYRAANYYLKKTLTTRRGAP
                     WLAESVREQALVCQHWLRDALAAPFDGPTVAVTHFAPSLRSADPRYGQVPGTAGFCNA
                     LDDLLPHAKVWLHGHLHAPSDYVHQGCRVVANPLGYARKNEQAAFLPGILIEIGA"
     gene            complement(2911..>3479)
                     /locus_tag="PKX82_09440"
     CDS             complement(2911..>3479)
                     /locus_tag="PKX82_09440"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HOF52518.1"
                     /translation="SVVRQQGADDAAWKTLRFMVFDVPAHGGPFSERITALQAAVARI
                     GQPWVQAVAQSRGTTGHALQERLATTVQSGGEGLMLHHGAARYAAQRSQDLRKLKPFD
                     DAEAQVVGHVAGRGKHAGRLGALWVEIPAQEGQAPRRFKLGTGLSDAQRDTPPPVGTW
                     VTYRYRGQTDSGLPRFASFLRIASDRAL"
BASE COUNT          646 a         1152 c         1066 g          615 t
ORIGIN      
        1 ctacaacatg agcgactact tccagcactg gctggacatg gagcacaagc tcgaagaatc
       61 cggccacacc ctgcccaaaa tctactgcgt caactggttc cgcaagggcg acgacggcaa
      121 gtttgtctgg cccggttatg gcgacaatat gcgtgtactg aagtggatga ttgaccgcct
      181 ggaaggcacc gcccaaggca cagctaccgg ctttggcgtc gctccccagt actccgaaat
      241 caactggaca ggtctggact tcagccaggc ccagtttgac tccgtcacca gcctggacaa
      301 agccgcatgg aagcaggaat ttgccctgca cagcgaactg ttcacccagc tggcctacca
      361 tttgcccaaa gaactcgaag agacaaaagc aaaactggaa gcaagcctca ccgcctgatt
      421 tccgacagac aacaaaaaag ccaccgcatg cggtggcttt ttttatgggc agaaacaatt
      481 aacgtgaagc tgcgcgcaac gggtcgaagg gcaaggcgct gtcatgctca cgcatccagg
      541 ccatctgcag ttcagccatc acgcggggat cgcgctgggc gtaatcccac atggcctctt
      601 cctggcggcg cagggcagcg gcacgtgccc agcgcgcgta caacgcagcc atggcactgg
      661 tcccctggcg caatggtggc gccagcaacg ccagcgcagc aaaggccacg gtccacaggg
      721 ccacccagcc ggccagcagg tgaccgtcag cccaggtgtc aatgacttgg tccgctacca
      781 ccagcaaggc agccatgacc gctgccagca gcatgccagc cagcgtacgg gagccttcgg
      841 tatccttgct ctggccgccg taggtcgccg acggggcgcc cagagactcc gcaccggcct
      901 gcgatgcgaa gagaggttgg gtaaaacgtg ccatggtgga ctccttggac aggttgatgg
      961 aaatcggatt tggggtcata tgggatcgaa tactagggtt tttaccaata tgattcaact
     1021 ttatgttggt gattcaatac attcatgaga ctaatacttg agcatgccaa ccctgaacct
     1081 gcgtactttt gacctgaacc tgctcaaggt gtttgatgcc gtcatggccg agcgcagctt
     1141 gacccgggcg gcgcaccagt tgtcactcac ccagcccgcg gtgagcaatg cgctgcgccg
     1201 tttgcgcgag gccctgggcg acgatctgct catgcgcaag gggcgtggcc tggaacccac
     1261 cgtccgcgcg ctggagttgt ggcccgccgt acaggaagcg ttgcagaagt tgcaaacgtc
     1321 gctcgcaccc agcgtgtttg accccgccac cgccaccgcc accacgtcct tcgtgctgac
     1381 catggccgat gccactgcag ccgaactcat gcctggtctg gtggatgtca tcacccgcga
     1441 ggcgccaggc gtcaccctgc gtgtcgtccc gctgaccacc cgcgacccgc gccgcctgct
     1501 cgacgagggg caggccgacc tggccattgg ccactttccg gccgtgctgg ccgacctgac
     1561 cgcgcgcgcc cagtccggcg aagctgtgtc ttttctgcac caccggctgt ttgccggaga
     1621 ctatgtctgc gtcatgcgca aaaaccaccc gctcgcccag ggcccgctga cgctggacca
     1681 gtactgcgcc gcgcgccaca tgctggtgag cttttcgggg cgggcgtttg gctttatcga
     1741 cgaggccctg gcgctgttgc accgcaagcg cccggtggtg ctgacggtga accagttctt
     1801 caccgccggc aaggtggtcg cccattcgga cctgctcacg gtcttgccgc ggcactttgt
     1861 caacgtgacc ggattcgtgg accagctgga gcagcgcgaa ctgcccttcc aagtaccgcc
     1921 gatacaggtc gatgcacttt ggcaccagcg ccagaatggc tccagtgccc atgcatggct
     1981 acgtgcgcag gtggtggcgc tggcagacaa agcctttccg gcatgatgcg gtcatgaaga
     2041 ttcagctctt gtccgacctc cacctggaag cccacccgca ttggcgcgcg acgccggcgg
     2101 caggcgccga cgtgctggtg ctcgctggcg atgtcgggtc gtaccagccg ggctcgctgt
     2161 tgacggacag cgatttcggg ttggggcagt tctctgcggc atccggctgg cccacaccgg
     2221 tgatttttgt accgggcaac catgagtatg acggcatgga ctttgatgcg gcccacgaac
     2281 ggctgcgcgc cacctgtgcc cgcctggggt tgcactggct ggagcgcgag acgctcacgc
     2341 tgcacggtgt gcggtttgtc ggcacgacgc tgtggaccga cttcgatgcc ctggccccca
     2401 acgccgacca ctccacgacg gcactggcgc agcaactcaa ggcgcgggac aaggcctacc
     2461 gcgccgccaa ttattattta aagaaaacat tgaccacgcg ccgcggcgcg ccgtggctgg
     2521 cagaaagtgt gcgcgagcag gcgctggtat gccagcactg gctgcgcgac gcactggccg
     2581 cgcccttcga tggccctacg gtggcagtca cccactttgc ccccagcctg cgcagcgcgg
     2641 acccgcgcta cgggcaggta cccggcaccg ccgggttttg caacgcactg gacgacctgt
     2701 tgccgcacgc gaaggtgtgg ctgcatgggc acctgcacgc gcccagcgac tatgtgcacc
     2761 agggctgccg cgtcgtggcc aacccgctgg gctacgcgcg caaaaacgag caagccgcat
     2821 ttctgccggg cattttgata gaaatagggg cctagccccc gtggaatatg cgcaaacagc
     2881 tatctatttg atagcaaacc aactttggcc ctacaaggca cggtcactgg caatccgcag
     2941 aaagctggca aaccggggca ggccggagtc ggtctggccg cggtagcggt aggtcaccca
     3001 ggtccccacg ggcggagggg tgtcccgctg ggcatcactc aggccggtgc ccagtttgaa
     3061 gcggcgcggc gcctggccct cctgcgcggg aatttccacc cagagcgcac ccagccggcc
     3121 cgcgtgtttg ccgcggcccg ccacatggcc cactacctgc gcttcggcgt catcaaacgg
     3181 tttgagtttg cgcagatcct ggctgcgctg ggctgcatag cgtgcggcgc cgtggtgcag
     3241 catcaacccc tcgccgccac tctgcacggt cgtcgccaga cgctcctgca atgcgtgccc
     3301 ggtggtgccc cggctttgag ccaccgcctg cacccacggc tgaccgatcc gggccacggc
     3361 ggcctgcagt gcggtgatgc gctcgctaaa gggtccgccg tgcgcgggca cgtcaaacac
     3421 catgaagcgc agcgtcttcc atgcggcatc gtcggcgccc tgctgccgca ccaccgaga
//
LOCUS       DAOSJM010000263         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23652_length_3475_cov_3.961404, whole genome shotgun sequence.
ACCESSION   DAOSJM010000263 DAOSJM010000000
VERSION     DAOSJM010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23652_length_3475_cov_3.961404"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PKX82_09445"
     CDS             complement(<1..805)
                     /locus_tag="PKX82_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOF52519.1"
                     /translation="MNFELKPLGLAAVASEKCDALVVLIPEGFKPGKDDLSLLAGQSI
                     KDKDLETKPGKLLQLYRPVQASAVRLVLAGVGEGKAKQVRTALTAALASLKGPSLKKL
                     VVCFATPAREDAVRATVHAIADATYVYTTTKSKSEGRTLQKVLVTATNASEVHATFLR
                     ASATVPGVELAKEWANRPANHATPIELAKAAHELAKLPKVSCSVLGPKEVTKLGMGSF
                     LAVAQGSEEPLRFIVLRYDGAAKTQAPTVLVGKGITFDSGGVSIKPAAEM"
     gene            829..1944
                     /gene="lptF"
                     /locus_tag="PKX82_09450"
     CDS             829..1944
                     /gene="lptF"
                     /locus_tag="PKX82_09450"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOF52520.1"
                     /translation="MLFHSSVRKELARSFGATLVVLVTVVMTMTLIRTVGEASRGTFN
                     PTDVMIIMGYTVLSDMPTILSLSLFISILSVLTRMFGDSEMVIWFGSGRGLASLVKPL
                     LRFAWPILVVILALALFILPWAFGRIEDLRDKYEKRGDLARIEPGQFQESANGDRVFF
                     IEKDSQGRQAGNNVFIATNEQGKETITSAQSGRVEVIGTDKFLILENGQRLEKTVGKP
                     ELTMSVFQRYGARVGADDQSARSYVPSSSMTTLQLVQDPTNRHLAELSWRIGLGLAAF
                     NFVLIGLATAGVNPRVGRTANIGFAFMAFVVYFNFLVLGKNWVETGQVGFAPFLIALH
                     GGALALAALWLTKRHNNWTLRLPRRNRPTASQESSTL"
     gene            1941..3047
                     /gene="lptG"
                     /locus_tag="PKX82_09455"
     CDS             1941..3047
                     /gene="lptG"
                     /locus_tag="PKX82_09455"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOF52521.1"
                     /translation="MRTIRKLFYKEMVLAVALVTLGFIALFFFFDFVEELQAVGRSSA
                     LGYKIPQALVYVALMAPNHVYELLPITVLIGTIFVMARLAQSSEFTILRTSGLGPWRA
                     LRTLLVLGMGFVLFTFAVGDYIAPLADRTAQLLKAQFQGKVTVGKTGAWLKEKQAYGH
                     YAVNVGALAPDASMRDVRIFEFDNKGYLVSMTLGKTARFGENEEWNLEQVDRTEFTSA
                     DSSASHAQRLTTDSFRWPTQISAEMVAAAVLRPERMGTIDLFQYMRHLNANGQSAQKY
                     EIQFWKKVFYPLSCLVMVVLALPFAYLHFRSGSIATYVFGGVMAGISFVLLNNVLGDL
                     GTIQGWQPWFTAALPGLLYSLLSLTAFAWLVIRR"
     gene            3044..3424
                     /locus_tag="PKX82_09460"
     CDS             3044..3424
                     /locus_tag="PKX82_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOF52522.1"
                     /translation="MSTTSQKRGIVLFAHGSRDPLWHKPMEAVARCIQESQADAQVLC
                     AYLELSTPDLPTAVATLVERGIGAITIVPMFLGVGKHAREDLPQLVAQLKQAHSDVQF
                     SLQPAVGENPRLISLLADIALDKN"
BASE COUNT          667 a         1062 c          994 g          752 t
ORIGIN      
        1 ccatttcggc cgcaggcttg atggacacac cgccgctgtc aaaggtgatt cccttaccaa
       61 ccagcaccgt gggagcctgg gtttttgccg ccccgtcgta gcgcaacaca atgaaccgca
      121 gtggttcctc ggagccctgc gccacggcca ggaaggagcc catgcccagc ttggtgactt
      181 ctttgggccc cagcacgctg cagctcacct tgggtagctt ggccaattcg tgcgccgctt
      241 tggccaactc gatgggcgtg gcatggttgg caggccggtt cgcccattcc ttggccaact
      301 ccactcccgg caccgttgca ctggcgcgca ggaaagtcgc gtgcacttcc gatgcgttgg
      361 tcgccgttac cagcaccttt tgcagggttc gcccttcgga cttcgatttg gtcgtggtgt
      421 acacataggt cgcatcggca atggcgtgca ccgttgcccg caccgcgtct tcacgcgctg
      481 gcgtggcaaa acacacgacc agtttcttca aggaagggcc cttgaggctg gccagcgccg
      541 ccgttagcgc cgttcgcacc tgctttgctt tgccctcccc cacgccagcc agaaccagcc
      601 gcacggcgct ggcctgtact ggacggtaca gctggagcag ttttccgggt ttggtttcca
      661 gatccttgtc cttgatcgac tggcccgcca acagggacaa atcgtccttg ccgggcttga
      721 atccctcggg aatcaacacc accaaggcat cacatttttc gctggcaact gcggcaaggc
      781 ccaggggctt gagttcaaag ttcataattc aggttttcct tcgctgcaat gttattccat
      841 tcctctgtcc gcaaagaact cgcccgcagt tttggcgcca ccctggtggt gctggtgacg
      901 gtggtgatga ccatgacgct gattcgcact gtcggcgaag cctctcgcgg cacgttcaac
      961 ccgaccgacg tgatgatcat catgggctat accgtcctct cggacatgcc caccatcctg
     1021 tcgctgagcc tgttcatttc catcctgagc gtgctgacgc gcatgttcgg cgacagtgaa
     1081 atggtgatct ggtttggcag cggccgtggt ctggcgtccc tggtcaaacc gctgctgcgc
     1141 ttcgcctggc ccattctggt cgtcattctg gcgctggcgc tgtttatcct tccctgggca
     1201 ttcggccgca ttgaagacct gcgcgacaag tatgaaaaac gcggcgatct ggcgcgcatc
     1261 gaacccggcc agttccagga atccgccaat ggcgaccgcg tcttttttat cgagaaagat
     1321 tcacaggggc gccaggccgg caacaacgtg ttcattgcca ccaatgaaca gggcaaggaa
     1381 accatcacct ccgcgcaaag tgggcgggtg gaagtcatcg gcacggacaa atttctgatt
     1441 ctggaaaacg gccagcgact ggaaaagacc gtgggcaaac ccgaactgac catgagcgtg
     1501 ttccagcgct atggcgcacg tgtgggtgca gacgaccagt cggcacgcag ctacgttcct
     1561 tcaagctcca tgaccacact gcaactggtg caggacccca ccaatcgtca cctcgcagaa
     1621 ctgtcgtggc gcatcggtct ggggctggct gcattcaatt ttgtcctcat cggtcttgcc
     1681 acggcagggg tcaacccgcg ggtggggcgc accgccaaca tcggctttgc gtttatggct
     1741 ttcgtggtgt atttcaactt tctggtgctg ggcaagaact gggtggaaac cggacaggta
     1801 ggcttcgccc ccttcctgat cgccctgcac ggcggtgctc tggcactggc ggcgctgtgg
     1861 ctgaccaagc ggcataacaa ctggaccttg cggttgcccc gacgcaaccg gccaacggcc
     1921 agccaggaga gcagcacact atgagaacca ttcgcaagct gttctacaag gaaatggtgc
     1981 tggcggttgc gctggtcacg ctggggttta ttgccctttt cttctttttt gattttgtgg
     2041 aagagcttca ggccgtcggc cgaagcagcg cactgggcta caagattcca caggccctgg
     2101 tctacgtggc cctgatggca cccaaccatg tgtacgaact cctgcccatc acggtgctga
     2161 ttggcacgat tttcgtcatg gcccggttgg cacaaagctc ggagttcacc atcctgcgca
     2221 ccagtggact gggcccctgg cgcgcactgc gcacgctgct ggtactgggt atgggtttcg
     2281 tactcttcac ctttgcggtg ggcgactaca ttgcgccgct ggccgacagg actgcgcagc
     2341 tgctcaaggc gcagttccag ggcaaggtca cggtcggcaa gaccggggcc tggctgaagg
     2401 agaaacaggc ctatgggcac tatgcggtca acgtaggcgc actcgcgcca gatgccagca
     2461 tgcgcgatgt acgcattttc gagttcgaca acaagggata cctggtctcc atgaccctgg
     2521 gcaagacagc acgttttggc gaaaacgaag aatggaacct cgaacaggtc gaccgcaccg
     2581 aattcaccag cgcggattcc agtgcctccc acgcccagcg cctgactacc gacagcttcc
     2641 gctggccgac acagatcagt gccgaaatgg tggcggctgc cgtcctgcgc cccgagcgca
     2701 tgggcaccat cgatcttttt cagtacatgc gacacctcaa cgccaacggt cagtcggcgc
     2761 agaagtacga aatccagttc tggaaaaaag tgttttaccc cctgagctgc ctggtgatgg
     2821 tggtgctggc cctgcccttt gcctatctgc atttccgctc gggtagcatc gctacctatg
     2881 tgtttggcgg ggtgatggcg ggcatcagct ttgtgctgct caacaacgtg ctgggggacc
     2941 tgggtacgat tcagggatgg caaccgtggt ttacggctgc actgccaggg ctgctttatt
     3001 ccctactatc cctcaccgcc tttgcctggc tggtcatacg acgatgagta ccacatccca
     3061 aaaacgcggc attgtgctat ttgcccacgg ctcgcgcgat cccctttggc acaagcccat
     3121 ggaggccgtt gccaggtgca tccaggaatc acaggccgat gcacaggtgt tgtgcgccta
     3181 tctggaactc agtacgccgg atttgcccac cgcagtggca acgctggtag agcggggaat
     3241 tggcgccatc accatcgtcc ccatgtttct gggtgtcggc aaacatgccc gtgaagacct
     3301 gccacagctg gtggcgcaac tgaaacaagc ccattccgac gtgcagtttt ccctccagcc
     3361 cgctgtcgga gagaaccccc ggttgatctc gctactggca gacattgctc tggacaagaa
     3421 ctgaaaacca gcgcaaaccc atagatgatt gaggcataat gaatttcatc tttag
//
LOCUS       DAOSJM010000264         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_23864_length_3452_cov_3.488961, whole genome shotgun sequence.
ACCESSION   DAOSJM010000264 DAOSJM010000000
VERSION     DAOSJM010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23864_length_3452_cov_3.488961"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKX82_09465"
     CDS             <1..599
                     /locus_tag="PKX82_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF52523.1"
                     /translation="AADFIGALLDAAGVDKAALVGHSWGSLIALEAAARLKERVSHLV
                     LVGTAFPMRVSQMLLDASQSDPIKALTMVNVFSRSTLAPPPSALGPGTWVFGGGMALG
                     KRVLASNTAVNVFYRGFKACDSYANGENAMAQISCPILFILGEQDQMTQAKAAQPLVK
                     VAQSTGKAVTVTTVPVGHHQMTEAPDATLFAMRDFLQK"
     gene            complement(609..1769)
                     /locus_tag="PKX82_09470"
     CDS             complement(609..1769)
                     /locus_tag="PKX82_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HOF52524.1"
                     /translation="MDAAITLRERFREDRLGLTASSLTFTTTIALVPFITVALAVFTA
                     FPMFAKFQDVLQKWLIESLVPDNIARQVLGSLNQFAGKASKLGAAGLAVLLSTALALV
                     FTIDRTLNSIWRVRTPRPFGQRVLLYWAAITLGPLVLGASLSMTSYALSASRGLVGVM
                     PGGVKLLLEFLQFVLVAGGMAAMFRYVPNTHVRWGHAWMGGLFVSGCMEVAKKLLALY
                     LSQVPTYSVVYGAFATVPILLVWIYVAWIIVLLGAALTAYVPSLLAGLPRRRGGPGWQ
                     FQLALEVLQQLDAARKHSDKGRTIPQLCIALQTDSQRLEPILEALVALDWIGQLSETQ
                     GREAASRFVLLVDPQQTPLQPLVQSLLLPHGPATQNLWKNDRWPACSLCEVL"
     gene            1857..2249
                     /locus_tag="PKX82_09475"
     CDS             1857..2249
                     /locus_tag="PKX82_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HOF52525.1"
                     /translation="MHSSIKFTRWLAVGSVLGLIALGLAWELFLAPLRPGGSWLALKV
                     LPLCIPLPGLLKNRMYTYRWVSLVVWLYFTEGVVRAWSDRAPGNYLAMLELALCLSLF
                     TACTLHVRLRQRHAKAAGLTEPPVSAAP"
     gene            2246..>3452
                     /locus_tag="PKX82_09480"
     CDS             2246..>3452
                     /locus_tag="PKX82_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOF52526.1"
                     /translation="MTANHPLLATLRAIVGDANVLTEGDLSAWELDWRKRERGHALAV
                     VRPASTDEVAQVIKACAAAGVSMVPQGGNTGMVVGSTPDASGTQVVLSLTRMHRIRKL
                     DAANLTVTVDAGCVLQSIQEACEAQGFLFPLSLAAEGSCTIGGNLGTNAGGTQVVRYG
                     NARELCLGLEVVTAQGDVWNGLTGLRKDNTGYDLRHLFIGSEGTLGVITGAVMKLYPM
                     PAAQLTAFAAVPSLDAAVALLGLAHKHLHAGLTGFEVMGQFALSLVVKHFPQQRVPFW
                     QESPYCVVMEASDNETEAHARAVFERLLEAAMEQGCVTDAVVAENMAQAKALWHIRES
                     IPLAQAQEGLNIKHDISIAVSSIPEFVRQTDAALEAAFPGARLVNYGHLGDGNLHYNV
                     QCPEGSESLA"
BASE COUNT          663 a         1129 c         1060 g          600 t
ORIGIN      
        1 aggccgccga cttcattggc gcgctgctcg acgctgcggg cgtggacaaa gccgcactgg
       61 tcggccacag ctggggctcc ctcattgccc tggaagccgc cgcacgcctg aaagagcgcg
      121 tcagccacct ggtgctggtg ggtaccgcat tcccgatgcg ggtatcgcaa atgctgctgg
      181 acgcatccca gtccgacccc atcaaggcgt tgaccatggt caatgtgttt tcccgcagca
      241 cgctggctcc gccgccttcg gcactcggcc cgggcacctg ggtgtttggc ggcggtatgg
      301 cgctgggcaa gcgcgtgctg gccagcaata cggcagtcaa cgtgttctac cgcggcttca
      361 aggcatgcga cagctatgcc aacggggaga atgccatggc gcagatctcc tgccccatac
      421 tgtttattct gggggaacag gaccagatga cgcaggccaa ggccgcgcag ccattggtca
      481 aggtagcgca aagcacagga aaagctgtca ccgtgaccac cgtaccggta ggccaccacc
      541 agatgaccga ggcgcccgac gccactctgt ttgccatgcg cgacttcctg cagaaatagc
      601 tattattttt atagcacctc gcacagactg cacgcgggcc agcggtcgtt tttccataaa
      661 ttttgggtcg ctgggccatg tggaagcagg agagactgca ccaggggctg caatggcgtc
      721 tgctgtggat cgaccagcag cacgaagcgc gaggccgcct ccctgccctg ggtttcgctg
      781 agctgcccga tccagtccag tgctaccagc gcttccagga tgggctccag ccgctgcgag
      841 tcggtctgca aagcgatgca cagctgggga atggtgcgac ccttgtcgct gtgcttgcgc
      901 gctgcatcca gctgttgcag tacctccagc gccagctgga attgccaccc cggcccgcca
      961 cgccgccggg gcagacccgc cagcagactg ggcacatagg cagtgagtgc cgcccccagc
     1021 agcacgatga tccacgcaac ataaatccag accagcagaa tcggcaccgt ggcaaatgcg
     1081 ccgtacacca ccgagtaggt cggaacctgg ctcaggtaga gggccagcag cttcttggcc
     1141 acctccatgc agcccgaaac aaacaagccc cccatccacg catggcccca gcgcacatgc
     1201 gtgttcggaa cataacggaa catggccgcc atgcccccgg cgaccagcac aaactgcagg
     1261 aattccagca gcagcttgac gccgccaggc atgacgccca ccagccccct ggatgctgaa
     1321 agcgcatacg aagtcatgct caggctggca cccagcacca ggggtcccag agtgatggca
     1381 gcccagtaca gcagcacacg ttgcccaaac ggccgggggg tgcgcacccg ccagatgctg
     1441 ttgagggtac gatcgatcgt gaacaccagg gccaacgcgg tactgagcaa cacagccaga
     1501 ccggccgcgc cgagtttgct ggccttgccg gcaaactggt tgagcgaacc cagcacctgg
     1561 cgcgcaatat tgtccggcac caggctctcg atcagccatt tctgcagcac gtcctgaaac
     1621 ttggcaaaca tggggaaggc tgtgaatacc gcaagcgcga ccgtaatgaa tggcaccaac
     1681 gcgatggtcg tcgtgaaggt caggctgctg gcagtcaggc ccagccggtc ctcgcgaaag
     1741 cgctcgcgca gggtgatggc ggcatccatc cagggaacac gtcccacttc ttgcagccag
     1801 gaattgaatc gcgaaaagaa ttgcttgaag cccatgtcgc tatgatgcca ccgactatgc
     1861 actcctctat caaattcacc cgctggcttg ccgtgggcag tgttctcgga ctgatcgccc
     1921 tgggcttggc ctgggaactg tttctggccc cgctgcgccc cggcggctcc tggctggcac
     1981 tcaaggtgct gcccctgtgc attccgctgc ccgggctgct gaagaaccgc atgtacacct
     2041 accgctgggt cagtctggtg gtgtggctct atttcaccga aggcgtcgtg cgcgcctgga
     2101 gtgaccgcgc acctggcaac tacctggcca tgctggagct ggcgttgtgc ctgtccctgt
     2161 tcaccgcctg cacgctgcat gtgcggttgc gccagcgcca tgccaaagct gctggtctga
     2221 cggaaccccc agtgagtgcc gccccatgac tgccaaccac cccctgcttg ccaccctgcg
     2281 cgccatcgtc ggcgacgcga atgttttgac cgaaggcgac ctcagcgcct gggagctcga
     2341 ctggcgcaaa cgcgaacgcg gccacgccct ggccgtggtg cgccccgctt ccaccgatga
     2401 agtagcccag gtcatcaaag cctgcgctgc cgccggcgtg agcatggtgc cgcaaggcgg
     2461 caacaccggc atggtggtgg gatccacgcc ggatgccagc ggaacccagg tggtgttgag
     2521 cctcacccgc atgcaccgca tccgcaagct tgatgcggcc aacctgaccg tcacggtgga
     2581 cgcgggctgt gttctgcaat ccattcagga agcctgtgaa gcacaaggat tcctgtttcc
     2641 cctgagcctg gccgctgaag gcagctgcac gattggcggc aacctcggca ccaacgccgg
     2701 tggcacccag gtcgtgcgct acggcaatgc gcgcgagctg tgcctgggcc tggaagtggt
     2761 gacggcgcaa ggtgacgtgt ggaacggcct gaccggtctg cgcaaggaca ataccggcta
     2821 cgacctgcgc cacttgttca ttggctccga aggcaccctg ggggtcatca ccggtgccgt
     2881 catgaagctc tacccgatgc ccgccgcgca gctcacggcg tttgccgccg tgccgtcgct
     2941 cgatgctgca gtcgcactgc tcgggctggc acacaagcac ctgcacgccg ggctgaccgg
     3001 ctttgaagtc atgggccagt ttgcgctgag cctggtcgtc aaacacttcc cgcaacagcg
     3061 ggtgcccttc tggcaggagt cgccttactg cgtggtgatg gaggcctcgg acaacgaaac
     3121 cgaagcccat gcccgtgcgg tgtttgagcg tctgctcgaa gcggccatgg aacagggctg
     3181 cgtcaccgat gcggtggtcg cggaaaacat ggcgcaggcc aaagccttgt ggcatatccg
     3241 cgaaagcatt ccgctggcgc aggcgcagga aggcctgaac atcaagcacg acatctcgat
     3301 tgcggtgtcc agcattccgg agttcgtgcg ccagaccgat gcagcgctgg aagccgcttt
     3361 tcccggtgcc aggctggtca actacggcca cctgggtgat ggcaacctgc actacaacgt
     3421 gcagtgcccg gagggcagtg aatcactggc ct
//
LOCUS       DAOSJM010000265         3435 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_24011_length_3435_cov_3.671893, whole genome shotgun sequence.
ACCESSION   DAOSJM010000265 DAOSJM010000000
VERSION     DAOSJM010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24011_length_3435_cov_3.671893"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            729..1670
                     /locus_tag="PKX82_09485"
     CDS             729..1670
                     /locus_tag="PKX82_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOF52527.1"
                     /translation="MRQLKGVPKTHVYRIIRSGEVRVNKGRASAETRLNAGDVVRLPP
                     VRVSERAEQKSQSMAAQAGAYVPAKDFPILFEDDSFLAINKPAGVAVHGGSGVSFGVI
                     EQLRMARPQAKFLELVHRLDRETSGILLVAKKRSALKNLQDQFRERETGKTYLALVLG
                     TWPANKKVLDKPLHKYLLPGKGGEDGERRVKVVSKDDPDGMPSLTLVKVREASPSYSL
                     LEVTIKTGRTHQIRVHLASEGMPIVGDDKYGDFEVNKALARATGPGALKRMFLHAWRL
                     QCNHPATGERLELRADLPPELMQFLLEAMPATPPAPQ"
     gene            1693..2370
                     /locus_tag="PKX82_09490"
     CDS             1693..2370
                     /locus_tag="PKX82_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOF52528.1"
                     /translation="MTTSPKPRKRQFDLIAFDWDGTLFDSTAIITRSIQLAVGDVGGT
                     VPSDHEASYVIGLGLMQALAHAAPDVPQDKYPELGERYKFHYMRHQNDISLFKGVLPM
                     LDDLRARGHLLAVATGKSRKGLDEVLHAVELKGVFHASRTADETAGKPHPRMLHELMA
                     ELGVAPERVLMIGDTTHDLQLALNAGCASVGVSYGAHEPAAFDALSPRFIAHSVAELH
                     AWLLDHA"
     gene            2382..2762
                     /locus_tag="PKX82_09495"
     CDS             2382..2762
                     /locus_tag="PKX82_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOF52529.1"
                     /translation="MGTEFASQAIALCNSQALVNSGTAVPFDVVYCGQTCYAFAVRYA
                     DQVQAYLNRCTHVPMEMDYQPNQFFDLTGHWLICATHGAMYSPTTGACRSGPCRGGLV
                     KIAVSETDGVVHWHTAHNLKPVDF"
     gene            2779..>3435
                     /locus_tag="PKX82_09500"
     CDS             2779..>3435
                     /locus_tag="PKX82_09500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOF52530.1"
                     /translation="MPQAPVDSAQAAINSGANANAAGAPGWERSTLEKLAMAAIDEQR
                     AARRWRNGVRLAWLVFLAALVWLGMDRSGTSADVSKPHTAVVEIKGEIASGSEASAEL
                     IVAAMRGAFEDKGAQAVVLLINSPGGSPVQAGIINDEIHRLKALHNKPVYAVVEETCA
                     SAAYYIAAGADKIFVDKASIVGSIGVLMDGFGFTGLMDKLGVERRLLTAGENKAMLDP
                     F"
BASE COUNT          691 a         1010 c         1088 g          646 t
ORIGIN      
        1 gccttgtaga tattgccctt gcgctgttcg cgcccttcga tttcgatttc gtagtcgagg
       61 agtttttgtc cgtcgacaat ggccaggcgg cgttcttcag cctgcgtggc gttgatcagc
      121 atccgtttca tggagtgcgt ccttcatcag tagtacatgc ccaaagcggg cttatgacac
      181 ctgaactgac gagctgaaac gaggaggaat tgccggagaa cttcgactca aacgaggcgt
      241 cgaagcgtgg gcgcgtggag gggagcaagg ggatgcgttt gtgaatttgc tattgaatta
      301 atagctggtt gcgcccgatt gacgggggct acaggccgat ccatgccaat aaacggcacg
      361 aaaacgcgcc gcaggcaaaa gtggatcagg ctcaacaaca caaacatctt gcttcattcc
      421 gttgacagac ggtgggtctg caaactttgg gtattccgta tcagtgtttc aattcgtcgg
      481 cttgaccgta ctgcaggcag gctctcacag gcatttggcg tgtgatgggc gtgcgctaca
      541 acccggcggc atcgaccttc cagcgcgtct gtgtatggcg tgtggactaa actccagaca
      601 aatcaaccac ttacagcgca tcgctagtga aacacattat aggggccaat cccgctccac
      661 cgagtcccca agtgaaaacc ctaagcgtcg aggaagacgg ggcaggtcag cgcctggaca
      721 attacctgat gcgccagctc aagggcgtgc ccaagaccca cgtctaccgc atcatccgca
      781 gcggcgaggt gcgggtgaac aagggccggg ccagcgcaga aacccggctg aacgccggtg
      841 acgtggttcg cctgccgccg gtgcgggtgt ccgagcgggc cgagcagaaa agccagtcca
      901 tggcggcgca ggcgggtgcg tatgtgccgg caaaagactt ccccatcctg tttgaagatg
      961 acagtttctt ggccatcaac aagcctgccg gcgtggcggt gcatgggggc tcaggcgtga
     1021 gttttggcgt gatcgagcaa ttgcgcatgg cgcggccgca ggccaagttt ctggagctgg
     1081 tgcaccggct ggaccgggaa acctccggaa tattgctggt tgccaagaag cgcagcgccc
     1141 tgaaaaatct tcaggaccag ttccgggaac gggaaaccgg caagacctac ctggcgctgg
     1201 tgctgggcac ctggccggcc aacaagaagg tactggacaa gccgttgcac aaatacctgt
     1261 tgccgggcaa ggggggagaa gacggcgagc gccgcgtcaa ggtggtgtcc aaagatgatc
     1321 cggatggcat gccgtcgctg acgctggtca aggtgcgcga ggcatcgccc agctactcgc
     1381 tgctggaggt caccatcaag acggggcgca cgcaccagat ccgcgtgcat ctggcgtccg
     1441 agggcatgcc catcgtaggg gacgataaat atggggactt tgaggtcaac aaggcgctgg
     1501 cccgtgccac cggtcccggc gcactcaaac gcatgttcct gcacgcctgg cgcctgcagt
     1561 gcaaccatcc cgcaaccggt gagcggctgg agttgcgcgc cgatttgcct cccgagctga
     1621 tgcaattcct gcttgaggcc atgccggcca caccgccggc accccagtaa aaccagatac
     1681 gactcttttt gcatgactac ttctcccaaa ccgcgcaaac gccagttcga cctgattgcc
     1741 ttcgactggg acggcaccct gtttgactcc accgccatca tcacccgcag catccagctg
     1801 gcggtaggcg atgtgggtgg cacagtaccc agtgatcatg aggcctccta cgtgattggc
     1861 ctgggcctga tgcaggcgct ggcccatgct gcaccggacg tgccgcagga caaatacccc
     1921 gagctgggcg aacggtacaa gtttcactac atgcggcacc agaacgacat cagcctgttc
     1981 aagggcgtgt tacccatgct ggacgatttg cgcgcgcgcg ggcacttgct ggcggtggcg
     2041 acgggcaaga gccgcaaggg cctggacgaa gtgctgcatg ccgtggagct caagggcgtt
     2101 ttccatgcct cgcgcactgc cgatgagaca gcaggcaagc cgcatccgcg catgctgcac
     2161 gaactgatgg ctgaactggg cgtggcaccc gagcgcgtgc tgatgatcgg cgataccacc
     2221 cacgatctgc aactggccct caacgccggc tgcgccagcg taggcgtgag ttatggcgca
     2281 cacgagccag cggcattcga tgcgctgtcg ccccgtttta ttgcccattc cgtagccgag
     2341 ctgcacgcct ggctgctgga ccacgcctga aggagcccga agtggggacc gaattcgcat
     2401 cgcaggccat tgcactgtgc aactcgcagg ccctcgtcaa cagcggtacc gctgtgcctt
     2461 ttgacgtggt gtattgcggt cagacctgtt acgcctttgc ggtgcgctac gcagaccagg
     2521 tacaggccta cctcaaccgc tgtacccatg tgccgatgga aatggattac cagcccaacc
     2581 agttctttga cctgaccggg cactggctga tttgtgccac ccacggtgcc atgtacagcc
     2641 cgaccaccgg ggcctgccgt tccggcccgt gccgcggtgg actggtcaag attgccgtct
     2701 cggaaaccga tggcgtggtg cactggcata ctgcacataa cctgaaacct gtagattttt
     2761 gacatgaacg actcaaatat gccccaagcc cccgtggata gtgcgcaagc tgctatcaat
     2821 agcggagcaa atgccaatgc tgccggtgcg ccgggatggg agcgctccac gctggagaag
     2881 ctggccatgg cagccatcga cgagcagcgt gcggcgcggc gctggcgcaa cggcgtgcgt
     2941 ctggcgtggt tggtgtttct ggctgcgctg gtctggctgg gcatggaccg cagcgggacc
     3001 tcggccgatg tgtccaagcc gcacacggcg gtggtggaaa tcaagggtga aatcgcttcg
     3061 ggctcagagg ccagtgcgga actgatcgtg gccgccatgc gcggtgcgtt tgaggacaag
     3121 ggtgcgcagg ccgtggtgct gctgatcaac tcccctggtg gtagcccggt acaggccggc
     3181 atcatcaatg acgaaatcca ccgcctcaag gccctgcaca acaagccggt gtatgcggtg
     3241 gtggaagaga cctgcgcctc ggcggcctat tacatcgctg cgggtgccga caagatcttc
     3301 gtggacaagg ccagcatcgt gggcagcatc ggcgtgctga tggacggttt cggcttcacc
     3361 gggctgatgg acaagctggg tgtggaacgg cggctgctca ccgctggcga gaacaaggcc
     3421 atgctggacc ccttc
//
LOCUS       DAOSJM010000266         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_24162_length_3418_cov_5.157895, whole genome shotgun sequence.
ACCESSION   DAOSJM010000266 DAOSJM010000000
VERSION     DAOSJM010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24162_length_3418_cov_5.157895"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="PKX82_09505"
     CDS             <1..815
                     /locus_tag="PKX82_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52531.1"
                     /translation="LEPVPDAAIVLRGKGIRVAYPVPRPGLRGWFSKGRFVAVDGVDL
                     ELPAGQTLGVMGESGSGKSTLALAALGLQSHEGELEVLGSHWGQGAALDRALRKGVQV
                     VFQDPFSSLSPRMTVEEIVGEGLRVHAPELSQSQRRIQVQETLSAVGLMESQFPGLLQ
                     RYPHQFSGGQRQRIAIARALIVDPRLLVLDEPTSALDVTMAQQVLRLLQRLQRDRGLA
                     FLLITHDVEVIRAMAHRVAVMQQGHIVESGLVDAVLERPQHAYTRTLLAAAE"
     gene            962..1573
                     /gene="rimP"
                     /locus_tag="PKX82_09510"
     CDS             962..1573
                     /gene="rimP"
                     /locus_tag="PKX82_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOF52532.1"
                     /translation="MALQEIVEQTVTGLGYDLVEIERSAGGLLRITIDLPWVKPAEGS
                     VAPEQFITVEDCEKVTRQLQFALEVDGAEYKRLEVSSPGIDRPLRHVQDFERFVGQVV
                     DLTLKAPIGAAASGSVNANRKKFRGTLERVVAGDGSVGWQIVWSDAPEVKPGQKVSKK
                     RVPPPLQAMGFALDELREARLAPIVSFKGRGASGDVSGGGDAA"
     gene            1630..3126
                     /gene="nusA"
                     /locus_tag="PKX82_09515"
     CDS             1630..3126
                     /gene="nusA"
                     /locus_tag="PKX82_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOF52533.1"
                     /translation="MNRELLMLVEAISREKNVERDVVLGAVEAALAQATKKLYSGDVD
                     IRVALDRDSGNYETFRRWHVVPDEAGLQLPDQEILLFEAKEQIPDIEVDEYIEEAIES
                     VPIGRIGAMAAKQVILQKIRDAEREMLLNDFMSRGEKIFVGTVKRMDKGDIIVESGRV
                     EGRLRRSEMIAKENLRTGDRVRAMIMEVDLTLRGAPIVLSRSAPEFMIELFRQEVPEI
                     EQGLLEIKSCARDSGSRAKIAVLSHDKRVDPIGTCVGVRGTRVNAVTNELAGERVDIV
                     LWSEDPAQFVIGALAPANVSSIVVDEERHAMDVVVDEENLAIAIGRGGQNVRLASELT
                     GWKINIMDAAESAQKLAVETDSGRKLFMEKLDVDEEIADILIAEGFTSLEEVAYVPLQ
                     EMLEIESFDEDTVHELRNRAKDALLTMEIAHEESVEEVSQDLRDLQGLTPELIGKLAD
                     KGVHTLDDLADLAVDELTEITGQSADEAKTLIMKAREHWFTNDAASQE"
     gene            3151..>3418
                     /locus_tag="PKX82_09520"
     CDS             3151..>3418
                     /locus_tag="PKX82_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HOF52534.1"
                     /translation="MSSTTVAEFANELKKSTDTLLEQLKSAGVAKSAASDVLTDADKH
                     SLLNYLQSSHGTAGADRKKITLVKKQTTEIKQADSTGKARTIQVE"
BASE COUNT          712 a          922 c         1111 g          673 t
ORIGIN      
        1 tgttggagcc ggtgcccgat gcggccatcg tgttgcgcgg caagggcatt cgcgtggcct
       61 atccggtgcc gcgtcctggt ctgcgcggct ggttttccaa agggcgtttc gttgccgtcg
      121 atggtgtgga tctggagttg cccgcagggc agacgctggg tgtgatgggg gagtccggtt
      181 cgggcaagtc cacgctggcg ttggctgcgc tgggactgca gtcccatgag ggtgaactgg
      241 aggttctggg gagccactgg gggcagggcg ctgcattgga ccgcgcgctg cgcaagggcg
      301 ttcaggtggt ttttcaggac cccttttcat cactgtcgcc ccgtatgacc gttgaagaga
      361 tcgtgggaga aggcctgcgg gtgcatgcgc cggagttgag ccagagccaa cgccggattc
      421 aggtgcagga aaccttgagt gctgtggggc tgatggagag tcagtttcca ggcctgttgc
      481 agcgctatcc ccaccagttt tcgggcggcc agcgccagcg cattgcgatt gcacgagccc
      541 tcatcgtcga tccgcggttg ctggtgctgg atgagcccac cagcgccctg gatgtcacca
      601 tggcccagca ggtcctgcgg ctgctgcagc gcctgcagcg ggacaggggt ctggcgttcc
      661 tgttgatcac ccacgatgtg gaagtgattc gggccatggc ccatcgcgtg gccgtcatgc
      721 agcaaggcca tattgtggag agcggactcg tggatgccgt gctggagcgt ccgcagcatg
      781 cgtacacccg cacgctgctg gcggcagctg aataattcct taaaaatcaa ggcacttagc
      841 tcagaattgc agtacaatcc aaacctcacg accaaacggg cgggtaacta ccgcccgttt
      901 tttttggtcg attgtttttg catgtggtga attgaacgta gcctagaggg actgatcgaa
      961 cgtggcattg caggaaattg ttgaacaaac cgttaccggt ctgggatatg acctggtaga
     1021 gattgaacgc tctgccgggg gattgctgcg catcacgatt gacctcccct gggtgaagcc
     1081 cgcagaagga tctgtggcgc ccgagcagtt tattaccgtg gaagactgcg agaaggtgac
     1141 gcgccaattg cagtttgcac tggaagtcga tggcgcggaa tacaagcggc tggaggtctc
     1201 ctcgcccggt attgaccgtc ccttgcgcca tgtacaggat tttgagcgtt tcgtcggcca
     1261 agtggtggat ctgacgctca aggcgcccat tggagcggcc gctagcggct ccgtcaacgc
     1321 caaccgcaag aagttccgcg gcacgctgga acgcgtggtg gctggggacg gcagcgtggg
     1381 ctggcagatc gtctggagcg atgcaccgga ggtcaagccc ggtcaaaaag tgagcaagaa
     1441 gcgtgtgccc ccgccgctgc aggcgatggg ctttgcgctg gacgagttgc gcgaagcgcg
     1501 gctggcgccg attgtgagtt tcaagggccg gggtgcatcg ggtgatgtgt ccggtggtgg
     1561 tgatgcggcc taggtccgta ccgcagattt tgtttttgta ttgataagag tgttcaggag
     1621 agtcatggca tgaatcgcga actgttgatg ctggtagaag ctatttcccg tgaaaagaac
     1681 gtggaacgtg acgtggtgct gggtgccgta gaggcagccc tggcccaggc aaccaaaaag
     1741 ctgtactccg gtgacgtgga tatccgcgtg gcactggatc gcgatagcgg caattacgag
     1801 accttccgcc gctggcacgt ggtccccgat gaggctggcc tgcagttgcc tgaccaggaa
     1861 atcctgctgt tcgaagccaa ggaacaaatt cctgacatcg aagtcgacga atacattgaa
     1921 gaggccatcg agtcagtgcc gattggccgt attggcgcca tggcagccaa gcaggtcatt
     1981 ctgcaaaaaa tccgtgatgc cgagcgtgaa atgctgctca acgacttcat gtcgcgtggc
     2041 gagaagattt ttgtcggtac cgtcaagcgc atggacaagg gcgacatcat tgtcgaatcc
     2101 ggtcgtgttg aaggccgcct gcgccgcagc gagatgatcg ccaaggaaaa cctgcgtacc
     2161 ggcgaccgtg tgcgcgccat gatcatggaa gtggacctga cgctgcgtgg cgcgcccatc
     2221 gtgttgtcgc gttccgcccc cgagttcatg attgagctgt tccgccagga agtgcccgag
     2281 atcgagcaag gtctgctgga gatcaagtcc tgcgcccgtg actccggttc gcgcgccaag
     2341 atcgctgtgc tgtcgcacga caagcgcgtg gaccccatcg gcacctgcgt cggtgtgcgc
     2401 ggtacccgcg tcaacgccgt gaccaacgaa ctcgcaggtg agcgcgtcga tatcgtgttg
     2461 tggagcgagg atcctgccca gttcgtgatt ggtgcgctgg ctccggccaa tgtatctagc
     2521 atcgtggtgg acgaagagcg ccacgccatg gatgtggtgg tggacgagga gaacctcgca
     2581 atcgccatcg gccgcggtgg tcagaacgtg cgcctcgcgt ccgagctgac cggctggaag
     2641 atcaacatca tggatgccgc cgaatctgcg cagaagctgg ccgtcgagac cgattccggc
     2701 cgcaagctgt tcatggaaaa gctcgatgtg gacgaggaaa tcgccgacat cctgattgcc
     2761 gaagggttta ccagcctgga agaagtggcc tatgtgccgc tgcaggaaat gctggaaatc
     2821 gagagcttcg acgaagacac ggtgcatgaa ctgcgcaacc gtgccaagga tgcgttgctc
     2881 acgatggaaa ttgcccacga agaaagtgtg gaagaggtct cccaggacct gcgtgatctg
     2941 caaggattga cgcccgaact gatcggcaaa ctggcagaca aaggcgtgca tacgctggac
     3001 gatctggcgg acctcgccgt tgacgaactc acagaaatta cgggccagtc tgcggacgaa
     3061 gccaagaccc tcatcatgaa ggcgcgcgaa cattggttta ccaatgacgc cgcttctcaa
     3121 gagtaacggt catgaaaggt atcagcacaa atgtccagta cgaccgtagc cgagtttgcc
     3181 aatgaactca agaaatccac cgacacgctg cttgagcagc tgaagtcggc cggggttgcc
     3241 aaatcggcag cctccgatgt cctcacagac gccgacaagc acagcctgtt gaactacctg
     3301 caaagcagcc acggcactgc cggggcggat cgcaagaaga tcacgctggt caagaaacag
     3361 accaccgaaa tcaaacaggc cgactccacc ggcaaggcac gcaccatcca ggtcgagg
//
LOCUS       DAOSJM010000267         3381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_24506_length_3381_cov_3.786530, whole genome shotgun sequence.
ACCESSION   DAOSJM010000267 DAOSJM010000000
VERSION     DAOSJM010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24506_length_3381_cov_3.786530"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PKX82_09525"
     CDS             <1..213
                     /locus_tag="PKX82_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HOF52535.1"
                     /translation="FEAVSWLDDPTSVTSLALIPLRETVEGGVTPAFGMLVLASMDAQ
                     RFDSGMGTDFLARIGQLASAALSRLR"
     gene            218..1186
                     /locus_tag="PKX82_09530"
     CDS             218..1186
                     /locus_tag="PKX82_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOF52536.1"
                     /translation="MDADAGLVERYLEYARVEKRLAARTVELYALDLQRLSGLAAQAQ
                     VPLLEVRNAHIRRWVAQMHSAGRSGRGIALILSGWRGFYTWLGRDGLVTSNPVQDVRS
                     PKAPKPLPKALGVDDAVQFADFQSDDDPWLEARDTAMVELLYGSGLRVGELVGLDVVA
                     SSTARGWVDLQAAQAHVLGKGSKRRIVPVGSTALQALERWLALRGPTGTSRAVPVPEA
                     HADAAAALFTGRNGTRLTPQSVWQRLKRRSQMAGLSTPVHPHMLRHSFASHVLQSSGD
                     LRAVQELLGHANITTTQVYTRLDFQHLAKAYDAAHPRARAKGSKGG"
     gene            complement(1187..2332)
                     /locus_tag="PKX82_09535"
     CDS             complement(1187..2332)
                     /locus_tag="PKX82_09535"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF52537.1"
                     /translation="MTEPETPEDQAQLERIRIFFGHAKGNLMSLLLGGAIIDAVLHSG
                     GTSLLAILCWDVVLVLAVIAAWMLESHVLRTGVSRDNAQGFYKLRVRMGALISFVYGV
                     SVFLLPTQDTHAEHTFLFMIMSTVVTTVALGYAVMPSYYLTINVAAMAPSIVLFLFLY
                     ATARDSYYLLMVFVSLVWQFVILRKAHLVSRTVIDSISINLRLQQEIEEHKRTREAIS
                     YMAMHDDLTGLANRRYFEQVFERTLNQAGRSQSCFGLLSIDLNDFKPVNDTHGHATGD
                     HLLKIVAERLRKATRAGDFCARIGGDEFAVLCSPIKDPTDLDTITRNLKQALSSPFAP
                     DDIVVPTSASIGWAAYPADGDNLTRLSACADQRMYADKQEQKAASRA"
     gene            2404..2652
                     /locus_tag="PKX82_09540"
     CDS             2404..2652
                     /locus_tag="PKX82_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HOF52538.1"
                     /translation="MHTPQNPTSQPRNPLHGKTLEAIVTELVAHFGWAELGERIPVRC
                     FTHDPSVGSSLKFLRKTPWAREKVESLYLYMLRAQRRG"
     gene            2975..3211
                     /locus_tag="PKX82_09545"
     CDS             2975..3211
                     /locus_tag="PKX82_09545"
                     /inference="COORDINATES: protein motif:HMM:NF018181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DfrB family trimethoprim-resistant dihydrofolate
                     reductase"
                     /protein_id="HOF52539.1"
                     /translation="MEQSSVSVGISHDYQIQPPLPATFGLGERVRKKSGAAWQGHIVG
                     WYSTKLTPEGYAVESEAHPGSVQIYPVSALERVA"
BASE COUNT          648 a         1012 c         1083 g          638 t
ORIGIN      
        1 tttgaagcgg tcagctggct ggacgacccg acctccgtca cgtcgctggc gctgattcca
       61 ctgcgggaaa ccgtcgaagg cggtgtgacg cctgcgtttg gcatgctcgt gctggcctcc
      121 atggatgcgc agcgttttga cagcggcatg ggcaccgact tcctggcgcg cattggccaa
      181 ctggccagtg ccgcgctttc ccgactgcgc taaaccgatg gacgcagatg ccggtctggt
      241 cgagcggtat ctggagtatg cgcgtgtcga aaagcgcctg gcggcgcgca cggtcgagtt
      301 gtatgcgctt gacctgcagc ggctcagcgg cctggccgcc caagcgcagg tgcccctgct
      361 ggaggtgcgc aacgcccaca tccggcgctg ggtggcgcaa atgcacagcg cgggccgcag
      421 cgggcgcggc attgcgctga ttctttccgg ctggcgcggg ttctacacct ggctgggccg
      481 ggatggtctg gtgaccagca acccggtgca ggacgtgcgc tcgcccaagg cacccaaacc
      541 cctgcccaag gcactgggcg tggatgacgc ggtgcagttt gccgactttc agagtgacga
      601 cgatccctgg ctcgaagccc gggacacggc catggtcgag ctgctgtacg gcagtggctt
      661 gcgggtgggc gagctggtgg ggctggatgt ggtcgccagc agcaccgccc gcggctgggt
      721 ggacctgcag gcggcgcagg cccatgtgct gggaaagggt tccaagcgcc ggatcgtgcc
      781 ggtgggtagt accgcactcc aggcgctgga gcgctggctg gcgctgcggg ggccaaccgg
      841 cacctcgcgt gcggtgcccg tgccggaagc acatgccgat gcagccgctg ccctgttcac
      901 cgggcgcaat ggcacgcggt tgacgccgca gtccgtgtgg cagcgcctca agcgccgcag
      961 ccagatggcg gggctgtcca caccggtgca tccgcacatg ctgcgccatt cgtttgccag
     1021 ccacgtgctg caaagcagtg gcgacctgcg cgcggtgcag gagctgctgg gccacgccaa
     1081 tatcaccacc acgcaggtct acacccggct ggacttccag cacctggcca aggcctacga
     1141 tgcggcgcat ccgcgcgcac gcgccaaggg cagcaagggc gggtaatcag gcgcggctgg
     1201 cggccttctg ttcctgtttg tcggcgtaca tgcgctggtc tgcacaggca gacagtcgcg
     1261 tcaggttgtc gccatcggcc ggataggcgg cccagccgat actggcgctg gtcggtacca
     1321 cgatgtcatc gggcgcaaat ggactggaca gcgcctgttt caggttgcgg gtgatggtat
     1381 ccagatcggt ggggtccttg atcggactgc acagcactgc gaactcatcc ccgccgatgc
     1441 gggcacagaa atccccggca cgggtggcct tgcgcagccg ctcggccaca attttcagca
     1501 gatggtcgcc tgtggcatgg ccatgcgtgt cattgaccgg cttgaagtcg ttgaggtcga
     1561 tggacagaag gccaaagcag gactgtgatc gtccggcctg gttgagcgtg cgctcaaaca
     1621 cctgctcaaa atagcgccgg ttggccagcc ccgtcaggtc gtcatgcatg gccatgtagc
     1681 tgatggcctc gcgggtgcgc ttgtgttcct caatctcctg ctgcagccgc aggttgatcg
     1741 agatgctatc aatcactgtg cgtgacacca ggtgggcctt gcgcagaatg acgaactgcc
     1801 acaccaaaga cacgaaaacc atcagcaggt agtagctgtc gcgcgcggtg gcatacagga
     1861 acagaaacag cacgatgctt ggcgccatgg cagccacatt gatcgtcagg tagtaggacg
     1921 gcatgaccgc ataccccagt gccacggtgg ttacgaccgt ggacatgatc atgaacagaa
     1981 acgtgtgttc ggcatgggtg tcttgcgtcg gcagcaggaa tacggacacg ccatagacga
     2041 aggaaatcag tgctcccatc ctgacgcgca gcttgtagaa gccctgcgcg ttgtcacggg
     2101 aaactcccgt gcgcaacaca tggctttcga gcatccatgc cgcaatcacg gcaagcacca
     2161 ggaccacatc ccagcagagt attgccagaa gggatgtgcc gcccgagtgc agcacggcgt
     2221 cgatgatggc gccgccgagc aacaggctca tgaggttgcc cttggcgtgg ccaaagaaga
     2281 tgcggatgcg ctccagctgg gcctggtctt cgggtgtctc tggttcagtc atgtatccca
     2341 cacaaccttt ttccttgttt gcgacactat aaaccgccgg gagcccaatg ccacaatagc
     2401 gccatgcaca cgccccagaa tcccaccagc caaccccgca acccgttgca tggcaagacg
     2461 ctggaggcca tcgtgaccga gctggtggcg cactttggct gggctgaact gggtgagcgc
     2521 atcccggtgc gctgcttcac ccatgacccc agcgtcgggt cgagcctgaa atttctgcgc
     2581 aagacgcctt gggcacgtga gaaggtggaa agtctgtacc tgtatatgtt gcgcgcgcag
     2641 cggcgcgggt gactgcccga aaacttcccg tcagggctgg cagaccggag acccgttctc
     2701 tggcccacgc tcgccgtcag ctgttggggt tgtcctgcat gtgctggtca acaccttgtc
     2761 tggtgcacag actcggtggc aacggcgaat ggggccgacg cccgggcatc cgatctgcca
     2821 gcccagtggc gtggtggtta cggacataag atgcaaaatg gcttggcggg cggctgcaat
     2881 gcagcggaac ccgacattca tggaactcac ccaattgaac aaatgggaac tggcagatct
     2941 acaagttgta gcggctttca caaaaaggtt gaatatggaa cagagtagcg tttccgtcgg
     3001 aatatcacac gattatcaga ttcaaccgcc attgccagcg acgtttgggt tgggagaacg
     3061 cgttcgcaaa aagtcgggtg ccgcgtggca aggccatatc gtcggctggt acagcacgaa
     3121 actcacgcca gaaggctacg ccgtcgagtc cgaagcgcat ccaggctcgg tacagattta
     3181 cccggtctca gcgcttgagc gcgtggccta atcctttaat cgagaggact tcacccgata
     3241 cgccgggcgc tgcctctcat ctcagacatt gaagtcgcca atttcatgtt ttcaattact
     3301 gctgtctgga aacttggatg acggcaatca gtctgaagtg acctttgctt tgctgcgaaa
     3361 gcggtctctt ccatgtagct a
//
LOCUS       DAOSJM010000268         3371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_24614_length_3371_cov_4.123643, whole genome shotgun sequence.
ACCESSION   DAOSJM010000268 DAOSJM010000000
VERSION     DAOSJM010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24614_length_3371_cov_4.123643"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /gene="prs"
                     /locus_tag="PKX82_09550"
     CDS             complement(<1..419)
                     /gene="prs"
                     /locus_tag="PKX82_09550"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOF52540.1"
                     /translation="MQAPTPPDFMVFTGNANPGMAAEIARHLGISLGSATVGRFSDGE
                     VTVEINQNVRARDVFVVQSTCAPTNENLMELLIMVDALKRASAERISAVIPYFGYARQ
                     DRRPRSSRVPITAKVVANMLQAVGVARVLTMDLHADQ"
     gene            complement(496..572)
                     /locus_tag="PKX82_09555"
     tRNA            complement(496..572)
                     /locus_tag="PKX82_09555"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(536..538),aa:Gln,seq:ttg)
     gene            complement(630..1505)
                     /gene="ispE"
                     /locus_tag="PKX82_09560"
     CDS             complement(630..1505)
                     /gene="ispE"
                     /locus_tag="PKX82_09560"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOF52541.1"
                     /translation="MQSLYDIPAPAKLNLFLHITGRRDDGYHLLQSVFMLIDWSDTLH
                     FERRTDGQISREDLTTPLPPEDLCTRAARALQQATGCAEGAHIGVAKSVPAQAGMGGG
                     SSDAASTLLALNRLWNCNLSLEALQRIGLSLGADVPFFLCGHHAWVEGIGEKITPMAL
                     PKADLLVVKPDAGLDTRMIFSDPTLKRDSEPAIISGFAANAFGNTFRNIFEYGRNDLQ
                     AVAQRLCPGVNQALTWLESQGLNGRMTGSGSAVFAHLPHGVLWDSANLSESFQVKQCS
                     SLDAHPLVGWAHSDG"
     gene            complement(1539..2045)
                     /locus_tag="PKX82_09565"
     CDS             complement(1539..2045)
                     /locus_tag="PKX82_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HOF52542.1"
                     /translation="MTRVQRTWLQLLLLLAIVFIAGCASVPGARAPNDSETAHWQGRL
                     ALKVYSTPVQAMTANFELQGSAQAGSLVLTTVLGSTLARMQWDATSATLQSSNGGVQQ
                     FESLSALVRHATGTDLPVTSLFAWLQGQPAVAQEWEADLSEIPHGRLNARRLSATNAA
                     ELKIILDQ"
     gene            complement(2045..3208)
                     /locus_tag="PKX82_09570"
     CDS             complement(2045..3208)
                     /locus_tag="PKX82_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOF52543.1"
                     /translation="MRLLAGNTDGALNAARRGTTFNAQSDEIATLALNLWDAKAPAAE
                     DMVQKYMQGKAQPELRLEYARRLAVAKRVPEAYSQTQQVTRELPGLADGWLVLGSLEF
                     QNRQFDAATTSLKKYVELRRSSPAEAAQGTERGVVQAYLLLSQMAEQGQRFDEALAYL
                     QNIDSPAEAVRIQGRKASLLARQGKMAEARALLRDMPEKQPEDARTKVMAEVQLLRDY
                     KQHNEAYAVLVEAAKRSPEDVELMYEQAMAAEKIGKPAEMESLLRQVIATKPDYHHAY
                     NALGYSLADRNTRLTEARALIGKALELAPQDPYIMDSLGWLEFRSGNLQEALRILNDA
                     YKARPDAEIAAHLGEVLWNLNRRDDATSLWAEGKALNPDNETLLETMRRLTKP"
     gene            3207..>3371
                     /locus_tag="PKX82_09575"
     CDS             3207..>3371
                     /locus_tag="PKX82_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor Sua5"
                     /protein_id="HOF52544.1"
                     /translation="MVPTAAHAAAGLGQDVDLILDGGATPLGLESTVIGFDGDRPVLL
                     VLGGSQGAREI"
BASE COUNT          626 a          975 c         1045 g          725 t
ORIGIN      
        1 atctggtctg cgtgcaggtc catggtcagc acgcgggcca cgcccacagc ctgcagcatg
       61 ttggcgacca ctttggcagt aatcggcacg cgactggagc ggggacggcg gtcctggcgg
      121 gcgtagccga aataggggat gacggcgctg atgcgttcgg ccgatgcacg cttgagcgca
      181 tcgaccatga tcagcaattc catcaggttc tcgttggtgg gcgcgcaggt actctgcacc
      241 acgaacacgt cacgggcacg cacgttctgg ttgatttcaa cggtgacttc accatccgag
      301 aagcggccta cagtggccga ccccagggaa atgccgagat ggcgggcaat ttccgcagcc
      361 atgccaggat tggcattgcc ggtgaaaacc atgaaatccg ggggggtagg tgcctgcatg
      421 cgggttccag cgaagaaatt gggtttatgt cgtcacaatg cgcgcagtgt tgccggacca
      481 gcccgaacaa caatttggca ggggaggaag gattcgaacc ttcgcatgct ggaatcaaaa
      541 tccagtgcct taaccaactt ggcgactccc ctacacaggt tgacagctaa aagctgccca
      601 ccgctaaacc gtttagctaa accatttatt tagccgtcgc tgtgtgccca ccccaccaga
      661 ggatgagcat ccaaactgct gcattgcttg acctggaatg actcggatag gttggccgaa
      721 tcccagagca caccatgcgg caagtgcgca aacaccgcac ttccagagcc tgtcatccgt
      781 ccattcagtc cctggctttc aagccacgta agtgcttgat tgacaccggg acaaagtctc
      841 tgggcaacgg cttgcaagtc atttcggcca tactcaaaaa tgtttctgaa tgtatttccg
      901 aaagcgttcg cagcgaagcc tgagattata gcaggctctg agtcgcgttt caacgtcggg
      961 tcagagaaaa tcatccgggt gtccaatccg gcgtctggtt tgaccaccag aaggtcggct
     1021 ttgggaagtg ccatgggagt gattttttcc ccgatgcctt ccacccacgc gtggtggcca
     1081 cacaggaaga aagggacgtc tgcgcccaat gacagtccaa tgcgctgcag ggcttcaagg
     1141 gacaaattgc agttccacaa ccggttgagc gccagcagcg tggatgctgc atccgatgag
     1201 ccgccaccca tgccggcctg tgccgggacc gatttggcca cgccgatatg cgcgccttct
     1261 gcgcagcctg tggcctgctg cagggcgcgt gctgcgcgtg tgcacaggtc ttctggcggg
     1321 aggggtgtgg tcaggtcttc gcggctgatc tggccgtcgg tgcgtcgctc aaaatgcagc
     1381 gtatcgctcc aatcgatcag catgaagacc gattgcagca aatggtagcc gtcatcgcgc
     1441 cgcccggtga tgtgcaggaa caggttcagc ttggccgggg caggaatgtc gtaaagcgac
     1501 tgcatggcaa gattatcggg tgcgcaggtg gttgccgttt actggtcgag gatgatcttg
     1561 agctctgccg cattggtggc cgacaggcgc cgggcgttga gccggccatg ggggatttcc
     1621 gaaagatcgg cttcccattc ctgcgcgact gccggctggc cttgcagcca ggcgaacaga
     1681 ctggtcacgg ggaggtctgt acccgtggca tggcggacca atgcagagag ggattcaaat
     1741 tgctgcacgc ccccattgct ggattgcagt gtggcggaag tagcatccca ttgcatacgc
     1801 gccagtgtgc tgcccagtac ggtcgtcagc accagactgc ccgcctgggc actgccctgg
     1861 agttcgaaat tggccgtcat ggcctgtacc ggtgtgctgt agaccttgag ggccaaccgg
     1921 ccttgccaat gggcggtttc ggagtcattt ggggctctgg cccccggaac gcttgcgcaa
     1981 ccagctataa aaacaatagc aagcagcagc aagagctgca gccatgtgcg ctgaacccga
     2041 gtcatcacgg cttggtcagc cggcgcatgg tttccagcag ggtttcattg tcggggttca
     2101 atgccttgcc ttcggcccag aggctggttg cgtcatcgcg gcggttcagg ttccagagca
     2161 cctcacccag atgggccgca atttctgcat cggggcgcgc cttgtaggcg tcattaagga
     2221 tgcgcagagc ttcctgaaga ttgccgctgc ggaactccag ccagcccagg ctgtccatga
     2281 tgtaggggtc ttggggtgcc aattccagcg ccttgccgat cagtgcacgt gcctccgtca
     2341 gtcgcgtatt gcgatccgcc agcgaatacc ccagtgcgtt gtaggcatgg tggtagtccg
     2401 gcttggtggc aatcacctgg cgcagcaggc tttccatttc ggctggtttg ccaatctttt
     2461 ctgcagccat ggcctgttcg tacatcagct ccacatcctc gggcgagcgt tttgccgctt
     2521 cgaccagcac ggcgtaggcc tcgttgtgct gtttgtagtc tctcagcaat tgcacctcgg
     2581 ccatcacctt ggtccgtgcg tcttcgggtt gtttttccgg catatcgcgc agcagtgccc
     2641 gcgcttctgc catcttgccc tggcgtgcca ggagcgatgc tttgcgaccc tggatgcgca
     2701 ctgcttccgc cgggctgtca atgttttgca ggtaggccag tgcctcgtca aagcgctgcc
     2761 cctgttctgc catctgggac agcagcagat aggcctgaac tacgccgcgc tctgtgcctt
     2821 gtgccgcttc tgcgggggaa gacctgcgca attccacata ttttttgaga gaggtggttg
     2881 ctgcgtcgaa ttgccgattc tggaactcca gcgaccccag taccagccag ccgtctgcga
     2941 ggccgggcaa ttcacgtgtg acctgctggg tctggctgta ggcttccggc acacgcttgg
     3001 caactgcgag gcgtcgtgcg tactccagac gcaactcggg ctgtgccttg ccctgcatgt
     3061 acttttgcac catgtcttcg gctgcgggag ccttggcatc ccacagattc agggccagtg
     3121 tggcgatttc atcggactgc gcattgaaag tggtgccccg gcgtgcggca ttgagcgcgc
     3181 cgtcggtgtt gcccgcaaga aggcgcatgg tgcctactgc ggcccatgcg gcagccgggc
     3241 tgggccaaga cgtcgatctc attctcgatg ggggcgccac gccgctcgga ctggaatcca
     3301 ccgtgatcgg atttgacggg gacaggccgg tgctgctggt cctgggggga agccaggggg
     3361 cccgggagat c
//
LOCUS       DAOSJM010000269         3365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_24669_length_3365_cov_3.766767, whole genome shotgun sequence.
ACCESSION   DAOSJM010000269 DAOSJM010000000
VERSION     DAOSJM010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24669_length_3365_cov_3.766767"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PKX82_09580"
     CDS             complement(<1..603)
                     /locus_tag="PKX82_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOF52545.1"
                     /translation="MENVQAALWVVMLLVALALLFDFMNGFHDAANSIATVVSTGVLK
                     PGQAVLFAAFFNFIAIFVFHLSVAATVGKGIVQPGVVDTHVVFGALVGAITWNVITWY
                     YGIPSSSSHALIGGIVGAVIAKAGAGALVSAGILKTVAFIFVSPLLGFTLGSVMMVIV
                     AWTFRRARPSRIDKWFRRLQLVSAGAYSLGHGGNDAQKTIG"
     gene            complement(624..1271)
                     /locus_tag="PKX82_09585"
     CDS             complement(624..1271)
                     /locus_tag="PKX82_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HOF52546.1"
                     /translation="MLFGKLLPREGNFFEMFNQHADRIVEAAQAFSKLVANYSDLDLR
                     AKYNQEVDDAERAADRVTHDVNKAIHITFITPIDREQIHSLINTMDDVADLIQDSAET
                     MALYDVRHMTEEITRLTDLSVKCCERLRDAVKLLSHIADQSTAEAALKTCEEIDRLES
                     DADRVMRTAMSKLFREEPDVREVIKLKAIYELLETITDKCEDVANVIEGIILENS"
     gene            complement(1454..>3365)
                     /locus_tag="PKX82_09590"
     CDS             complement(1454..>3365)
                     /locus_tag="PKX82_09590"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HOF52547.1"
                     /translation="LRARGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPYVPNKRF
                     GGVCLGTKIAPIFYNTMEDAGALPIELDVNQMNMGDVVELRPYDGKAFKDGKEIASFQ
                     VKSDVLFDEVRAGGRIPLIIGRGLTAKAREALGLKPSTLFRLPQNPVDTKKGFSLAQK
                     MVGRACGLPEGQGVRPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSF
                     CHTAAYPKPVDVKMHHELPDFISTRGGVALRPGDGVIHSWLNRLLLPDTVGTGGDSHT
                     RFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQNGITLRDLVNAIPLYAI
                     KAGLLTVEKKGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVHLNKEPI
                     IEYINSNITMMKWMIANGYSDARTLARRIAAQEAWLKDPQLLKGDADADYAAVIEIDL
                     ADIHEPILACPNDPDDVKTLAEVAGTKIDEVFIGSCMTNIGHFRAASKLMEGKRDIPV
                     KLWIAPPTKMDAKQLSDEGHYGVLGGAGARMEMPGCSLCMGNQAQVKAGATVFSTSTR
                     NFPNRLGKDSNVYLGSAELAAICSKLGRIPTKEEYLADMGVLTAASDKVYQYLNFDKI
                     SDYTESAATVKA"
BASE COUNT          689 a         1000 c          989 g          687 t
ORIGIN      
        1 gccaatcgtc ttctgggcgt cattgccgcc gtgcccgagg ctgtatgcac ctgcggaaac
       61 aagctgcaga cgtcgaaacc acttgtcaat tcgggagggt cttgcgcgcc ggaaggtcca
      121 cgccacgata accatcatga ccgacccgag cgtaaaaccc agcaagggcg acacaaagat
      181 gaaggccacc gtcttgagaa tacctgctga aaccagtgcc cccgcgcctg ccttcgcgat
      241 cacggccccc acgattcctc cgatcaaggc atgtgacgaa ctgctgggta tgccgtagta
      301 ccaggtaatc acgttccagg tgatggctcc caccagtgca ccaaacacga cgtgggtatc
      361 cactacaccg ggctgcacaa tgcctttgcc gaccgtagcg gccacactca ggtgaaagac
      421 aaagatcgcg atgaagttga agaaggccgc aaacaacacg gcctgcccag gtttcaatac
      481 cccggtggac accaccgtgg cgatggagtt ggcggcatca tgaaaaccat tcatgaagtc
      541 aaacaacaaa gccagagcca ccagcaacat caccacccag agggctgcct gtacgttttc
      601 cataatgtct tacctggtcg gactcaggag ttctcgagga tgatgccctc gatcacattg
      661 gccacgtctt cgcacttgtc ggtaatggtc tccagcaact cgtaaatcgc cttgagcttg
      721 atcacttcgc gcacatccgg ctcttcgcga aacagcttgc tcatggcagt gcgcatcacg
      781 cgatccgcat ccgactccag gcggtcgatt tcttcgcagg tcttgagcgc cgcttcggcc
      841 gtggactggt ctgcaatgtg gctcaacagc ttgaccgcat cccgcagacg ctcacagcac
      901 ttgacgctca ggtcggtcag tcgggtgatc tcttcggtca tgtgccgcac gtcgtacagc
      961 gccatggttt cagccgagtc ctgaatcaga tcagccacat cgtccatggt gttgatcagc
     1021 gagtggatct gctcgcgatc aatgggggtg atgaaagtga tgtggatagc cttgttgaca
     1081 tcgtgcgtca cgcggtcagc agcgcgctct gcatcgtcca cttcctggtt gtatttggcg
     1141 cgcaggtcaa ggtcactgta gttggccacg agcttcgaga aggcttgcgc tgcctcgacg
     1201 atgcggtccg catgctggtt gaacatctca aaaaaattgc cttctcgcgg caacagcttt
     1261 ccaaacagca tggcgaactc ctatccttca aatgtgtcaa gaatatgacc ttggtatgac
     1321 taaggggaag tttaaccgcc caaccctgtc agcaagctgt gcacacacag gatccatcca
     1381 aataagccca tgaaaaaagg ccctgcagct tgcgccgcag ggccttgatg gggctttcag
     1441 gccagctacc ggattaggcc ttgacggtcg cagcagactc tgtgtagtcg ctgatcttgt
     1501 cgaagttcag gtactggtac accttgtcgc tggcagcggt caacacgccc atgtcggcca
     1561 ggtactcttc cttggtcgga atgcggccca gcttggagca gatggcggcc agttcggcgg
     1621 agcccaggta cacattgctg tccttgccca gacggttggg gaagttgcga gtcgacgtgg
     1681 agaacacggt agcgccggct ttcacctgcg cctggttgcc catgcacaaa ctgcaacccg
     1741 gcatttccat gcgggcacca gctccaccca gcacgccata gtggccttcg tcgctcaact
     1801 gcttggcgtc catcttggtc ggcggtgcaa tccacagctt gaccgggatg tcgcgcttac
     1861 cttccatcag cttggatgcg gcgcggaagt gaccgatgtt ggtcatgcag gaaccgatga
     1921 acacttcgtc gatcttggtg ccggcgactt cggccaatgt cttcacatcg tccggatcgt
     1981 tggggcatgc caggataggc tcgtggatgt cggccaggtc gatttcgatg acggcagcgt
     2041 agtccgcgtc ggcatcgccc ttgagcaact ggggatcctt cagccaggct tcctgggctg
     2101 caatgcggcg ggccaaggtg cgagcatcgg agtaaccatt ggcaatcatc cacttcatca
     2161 tggtgatgtt gctgttgatg tactcaatga tcggctcctt gttcaggtgc acggtgcaac
     2221 cggcggcgga acgctcggca gacgcgtcgg acaactcaaa tgcctgctcc accttcaggt
     2281 cgggcagacc ttcgatttcg aggatacggc ccgagaagat gttcttcttg ccctttttct
     2341 cgacggtcag caggccggcc ttgatcgcgt acagcggaat ggcgttgacc aggtcacgca
     2401 gggtgatgcc gttttgcatc ttgcccttga atcggaccag cacggactca ggcatgtcca
     2461 gtggcatcac gccggtagcg gcagcgaacg ccaccagacc agaacccgcg gggaagctga
     2521 taccgatggg gaaacgggtg tggctgtcgc caccggtgcc gacggtgtca ggcaacagca
     2581 gacggttgag ccaggagtgg atcacgccgt caccgggacg cagggccacg ccgccgcggg
     2641 tgctgatgaa gtcaggcagc tcgtggtgca tcttcacgtc cacaggcttg gggtaagcag
     2701 ctgtgtggca gaacgattgc atcaccagat cggcgctgaa gcccaggcac gccaggtctt
     2761 tcagctcgtc gcgggtcatc ggtccggtgg tgtcttgcga gcccaccgag gtcatcttgg
     2821 gttcgcagta ggtaccaggg cgcacgccct ggccttcggg cagaccgcag gcgcggccaa
     2881 ccatcttctg cgccagactg aaacctttct tggtatccac cgggttctgc ggcaggcgga
     2941 acagggtgga aggcttcaga cccagggctt cacgggcctt ggcagtcagg ccacggccga
     3001 tgatcagcgg gatacggcca ccggcgcgca cttcgtcaaa cagcacatcg ctcttgacct
     3061 ggaaggacgc gatctccttg ccgtccttga acgccttgcc gtcgtaggga cgcagttcca
     3121 ccacgtcgcc catgttcatc tggttcacat ccagttcgat gggcagagcg ccggcatctt
     3181 ccatggtgtt gtaaaagatg ggggcgatct tggtgcccag gcagacgccg ccgaaacgct
     3241 tgttcggcac atagggaatg tcttcgccag tgaaccacag cacagagttg gtggcggact
     3301 tgcgggaaga gccggtaccg accacgtcgc cgacataggc gaccaggttg ccacgggcac
     3361 gcagg
//
LOCUS       DAOSJM010000270         3360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_24711_length_3360_cov_5.678669, whole genome shotgun sequence.
ACCESSION   DAOSJM010000270 DAOSJM010000000
VERSION     DAOSJM010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24711_length_3360_cov_5.678669"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..2662
                     /locus_tag="PKX82_09595"
     CDS             392..2662
                     /locus_tag="PKX82_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF52548.1"
                     /translation="MGYAALAGLWIVLSDYIALGSATDAQTLSRFGFFKGLAFVTVTS
                     VTLYLLLRRWYGVQQRLHQRAKEALQSLEESEQRLVDAQSLAHVGSWHVVFGRTEAED
                     RWIISDELRHMFGKAIDFRMTTESSFVQMLPRDQVYARECWAAAKRGEGPFEWEYRIL
                     VDGRTRWLRVVARFQFDADGRAVSASGTDQDITERKEVEQELLIAATAFETQEAMFIT
                     DAHRTIVKTNKAFTEITGFSAEEAIGSNPSLLSSGRHNDIFYQTMWAEIDRNNCWRGE
                     IWNRRKNGEVYPEWLSITKVTSDDGVVTNYVAAFSDISQRKQAEETIYSLAFYDTLTG
                     LPNRKLLRDRLQQSLLLGMRTGHCGAVLFIDLDDFKSLNDTQGHAAGDALLIEVAERI
                     RKCVHTDDTIARLGSDEFVVVLDSLSTDGDLAAEQAKLVAERVHARIREPLTLAGQAY
                     RCKSCVGVSLFGAQATTVDELLKQADSAMSQGKQSGRDKVHFFDAQMQAALEERVRLE
                     YWLQQAVPTQLMLHFQAQTDARGRIFGAEALIRWQHPEKGLISPAAFIPLAESSGLIL
                     PIGQWVIASACRQLKVWERDARTRHLVLSVNVSARQFQQPGFVQEVEQALQETGADPG
                     LLKLELTESMLAENVDAMVAKMQQLRDRGVHFSLDDFGTGFSSLNYLKRLPIDQLKID
                     QSFVKDVLEDANDAAIVRSVIALGQSLGLAVIAEGVETQGQKDFLAVHGCAQFQGYLF
                     GRPLPLADFERQLQTA"
     gene            complement(2689..>3360)
                     /locus_tag="PKX82_09600"
     CDS             complement(2689..>3360)
                     /locus_tag="PKX82_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOF52549.1"
                     /translation="DAHGCELIGGDTTQGPLNLCITVFGEVPTGRALLRSGARPGDDI
                     YVSGTLGDASLALQCLLGKTALPDGMLARARERLEQPTPRVALGLALRGIATAAADVS
                     DGLLGDLGHILTQSRVGATIDTEIATTLIAACAYSTGATGQFGLEIPEAMQLQCVLAG
                     GDDYELVFTAPAHRRAAVVAAAEASATPVTRLGRIEAAVGLRLVDGQGQPVANTFASF
                     DHFAA"
BASE COUNT          612 a          969 c         1136 g          643 t
ORIGIN      
        1 gctgggcgtt ggccagcccc tggagcctgc cagctacgtg atcgtgcgcg aagtggctgt
       61 caccgattgg ggttacggag ggctgacgca ggcagcgcgg cgcagtggac cctccagcgg
      121 cggccgctaa attccctacc gaaacgcgcc ctggggatcc cggcagatgg gctggtcgaa
      181 ggatggaaat aatttcacaa aaatgttgcc ggtagggttt gtcggagagg catactctgc
      241 gctacttcgt ttcttcgggt ctgtggttgc gggtgcagtt gcaggggtcg cccgattcca
      301 tttcaaagac gagagtctcc agcacagtcg atgcccttgt ccacacggcc gcaagccgat
      361 ccaacgcata tcaccccccc tgcggattgc cgtgggctac gccgcactcg ccgggctgtg
      421 gattgtcctg tctgactaca tcgccctcgg gagcgccacg gatgcgcaga cgctgagccg
      481 gttcgggttt ttcaaggggc tggcttttgt tacggtgacc agtgtcacgc tgtacctgct
      541 gcttcgccga tggtacggtg tgcagcagcg cctgcaccag cgcgcaaagg aggctctgca
      601 atcattggaa gagagcgagc agcgcctggt ggatgcgcaa agcctggcac acgtgggtag
      661 ctggcacgtc gtgtttggca ggaccgaagc cgaggaccgc tggatcattt ccgatgagct
      721 gcggcatatg tttgggaagg ccatcgactt ccgcatgacg accgagagca gctttgtcca
      781 gatgctgccc agagaccagg tctatgcccg ggagtgttgg gcggcggcaa agcggggaga
      841 gggacccttt gaatgggagt accgcatcct cgtcgacggg cgcacccgct ggttgcgcgt
      901 ggtggcccgt tttcaattcg atgccgacgg ccgggccgtc tcggcgtcgg gcaccgacca
      961 ggacatcacc gagcgcaaag aggtcgagca ggagctgctc attgcggcga cggcctttga
     1021 aacccaggag gcgatgttca ttaccgatgc ccatcggacc atcgtcaaaa ccaacaaggc
     1081 tttcaccgag atcaccggct tcagtgccga agaggccatt ggcagcaatc catcgctgct
     1141 gagctctggc cggcacaacg atattttcta ccagacgatg tgggcggaaa tcgacaggaa
     1201 taattgctgg cgcggtgaga tctggaaccg gcgcaagaac ggggaggtgt acccggagtg
     1261 gctctcgatt accaaggtca ccagcgacga tggtgtggtg accaattacg tggcagcctt
     1321 ctccgatatt tcgcagcgca agcaggcgga ggagaccatc tacagcctgg ctttctatga
     1381 caccctgacg ggtctgccca accgcaagct gctgcgcgac cggttgcagc aatcgctgct
     1441 gctgggcatg cgtaccggcc actgtggcgc cgtgctgttc atcgatctgg atgatttcaa
     1501 gtccttgaac gatacccagg gccatgcggc gggcgatgcg ctgctgatcg aggtggccga
     1561 gcgcatccgc aagtgtgtgc acacggatga caccatcgcc cggttgggca gcgatgaatt
     1621 tgtcgtggtg ctcgacagtc tgagtaccga cggggatctg gcggccgaac aggccaagct
     1681 ggtggctgag cgggtgcacg caaggattcg ggagccgctg accctggcgg gacaggcgta
     1741 tcgctgcaag tcctgcgttg gggtgagttt gtttggagcg caggccacca ctgtggacga
     1801 gctcctcaag caggccgact ccgccatgtc gcagggcaag cagtccgggc gagacaaggt
     1861 gcacttcttc gatgcccaga tgcaggccgc actggaagaa cgggtgcgcc tcgaatactg
     1921 gctgcagcag gcggtgccga cccagttgat gctgcacttc caggcgcaga cggatgcacg
     1981 cgggcgtatc tttggtgcgg aagcgctgat ccgctggcag catccggaga aagggctgat
     2041 ttctccggcc gcctttattc cgctggcgga atcgagcggg ctgatcctgc ccatcgggca
     2101 atgggtgatc gcttcggcgt gtcgccagct caaagtgtgg gagcgcgatg ccaggacccg
     2161 gcatctggtg ctgtcggtca acgtcagcgc acgccagttt cagcaaccgg ggtttgtcca
     2221 ggaggtggag caggcgttgc aggagacggg tgccgacccg gggcttttga agctggagtt
     2281 gaccgagagc atgctggccg agaacgtgga cgccatggtg gccaagatgc agcagttgcg
     2341 ggaccgcgga gtgcatttct cgctggatga ctttggcacc gggttttcat ccctgaacta
     2401 cctcaagcga ctgcctattg atcagctcaa gatcgatcag tcttttgtga aggatgtgct
     2461 ggaggatgcc aatgacgcgg ccatcgtgcg ctctgtcatc gcactggggc agagtctggg
     2521 gctggcggtg attgccgagg gggtggaaac gcaggggcaa aaggactttc tggcggtgca
     2581 cggctgcgcg cagttccagg gttacctgtt tggccggccg cttcccctgg ctgacttcga
     2641 gcgacagctg cagaccgcct gaacgccctg tgcctggggg ctgacggttc aggcagcgaa
     2701 gtggtcgaag gaggcaaagg tgttggccac cggctggccc tgcccatcca ccaggcgcag
     2761 acccacagcg gcttcgatac ggcccaggcg ggtgactggc gtggcgctgg cctcggcggc
     2821 tgcgaccacc gcagcgcgcc gatgtgcggg tgcggtaaag accagttcgt agtcatcacc
     2881 gcccgccaga acgcattgca actgcattgc ttctggaatt tctaagccaa attggcctgt
     2941 agcccccgtg gagtatgcac aagcagctat taaagttgta gcaatttccg tgtcgatggt
     3001 ggcgcccacc cggctttgcg tcaggatgtg gcccaggtct ccgagcagtc cgtcgctcac
     3061 atccgccgca gcggtggcaa tgccgcgcaa ggccagtccc agggcgacgc gcggtgtggg
     3121 ctgctccagc cgttcgcggg cccgggccag catcccgtcg ggtagcgccg ttttgcccag
     3181 caggcattgc agtgccaggc tggcgtcgcc cagtgtgccg ctcacataga tgtcatcgcc
     3241 ggggcgggca ccactgcgca gcagggccct gccggtgggg acttcgccga acacggtgat
     3301 gcagaggttc agcgggccct gggtggtgtc accgccaatc agttcgcagc catgggcgtc
//
LOCUS       DAOSJM010000271         3338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_24912_length_3338_cov_3.820591, whole genome shotgun sequence.
ACCESSION   DAOSJM010000271 DAOSJM010000000
VERSION     DAOSJM010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24912_length_3338_cov_3.820591"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PKX82_09605"
     CDS             complement(<1..1177)
                     /locus_tag="PKX82_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOF52550.1"
                     /translation="MKALDRKLLRDLRLMWSQAITIALVVASGVTGFITSFTAYDALS
                     WSRDLYYTESRFADVFATLKRAPLSLERQLEAIPGAAHVETSITQIVPVDIPNVSDPI
                     VGRLIGLDVGEPQRLNRVALRAGRALETQHNGALEVLVSEGFAVARGLQPGDKLYALI
                     NGKREAMLIVGVALSPEYIFAGMGGSPDQRGFGVFWLDRHALAAAYNMEGAFNQVSVR
                     LAPQASEGQVIDSLNRVLAPYGGISAHGRDEQMSHMILNSEIKEQRVLGTVLPSIFLA
                     VAAFLLNVVLGRQIATQREQIAALKALGYDNRMIGAHYIKLVLVIVTVGVVVGLGMGA
                     LMGHWFTGIYADVFRFPELRYRVRPVLAFAAVSVSMAAALLATWNAIRATVQMAPAEA
                     "
     gene            complement(1174..1923)
                     /locus_tag="PKX82_09610"
     CDS             complement(1174..1923)
                     /locus_tag="PKX82_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF52551.1"
                     /translation="MQDTVTKAGAADPAVAVPVFFAERLTKTYQMGEVQVHALVDVDL
                     TINRGEFIVLLGPSGSGKSTLLNILGGLDRPSTGVSRFADHDLSAASDAELTRYRREH
                     VGFVFQFYNLIPSLTVRENVELVTDIAVNPMPATEALEMVGLADRLDHFPSQLSGGEQ
                     QRVAIARAIVKRPEVLLCDEPTGALDYVTGKLVLEVIERINRELGTTTIVITHNAAIA
                     GMADRILSLGGGRIQRVETNAHKLQPSELSW"
     gene            complement(1939..3006)
                     /locus_tag="PKX82_09615"
     CDS             complement(1939..3006)
                     /locus_tag="PKX82_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOF52552.1"
                     /translation="MKQVTLGQSGLSVTSICLGTMTFGEQVNEPLAHTILSHSLERGV
                     NFLDTAEMYAVPPKAETFNATEKIIGNWLEKNPGARQKLVIATKVAGPSRGMPWVRGG
                     SSDLTAADIIAACEGSLQRLKTDVIDLYQLHWPTRNVPMFGAMYYDPSRDNPALTSMH
                     AQLEALGTLVKAGKVRAIGLSNETPYGVHEFVRLAEQHNLPRVATVQNVYNLVSRALE
                     NGLDETMHHLGVSLLAYSPLAFGLLTGKYDVSGVDGPDAPKEGRIAKYESVRKQRWGR
                     KEAFAAAVRYNTLARENGMTPSQMALAFCYTKWQVASTIIGVTSVAQLDEDINAWGTT
                     LAPEVLKQIDAIRAEMRDPAC"
     gene            complement(3071..>3338)
                     /locus_tag="PKX82_09620"
     CDS             complement(3071..>3338)
                     /locus_tag="PKX82_09620"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOF52553.1"
                     /translation="MCAALVDAGGLELDMGTAFDDMTALSHHFSDKVSPEAQANRMHL
                     LTVMEDAGFEHIPHEWWHYALPGADHYPLVPSTHLGHLNPMLPL"
BASE COUNT          613 a         1085 c         1031 g          609 t
ORIGIN      
        1 tggcctcggc gggtgccatc tgcaccgtgg cgcggatggc gttccaggtc gccagcaggg
       61 ctgcagccat tgacaccgac accgcagcaa acgccagcac cgggcgcacg cggtagcgca
      121 gttccggaaa acggaacaca tccgcataga tgccggtaaa ccagtgcccc atcagcgccc
      181 ccatacccag ccccaccacc accccgaccg tcacgatgac cagcaccagc ttgatgtagt
      241 gcgccccaat catgcggttg tcataaccca gcgccttgag cgcggcaatc tgctcgcgct
      301 gggtggcaat ctgccggccc agcaccacgt tgagcagaaa tgccgctacc gcaagaaaga
      361 tgctcggtag caccgtaccc agcacccgct gctccttgat ctcggagttc aggatcatgt
      421 gcgacatctg ctcgtcacgc ccgtgcgcac tgatgccacc atagggtgcc aacacgcgat
      481 tgaggctgtc gatcacctgc ccttcgctgg cctgcggcgc cagccgcaca ctcacctggt
      541 tgaaggcgcc ttccatgttg taggccgccg ccagtgcatg ccggtccaac cagaacacgc
      601 caaagccgcg ctggtcgggc gatccgccca tgccggcaaa gatgtactcc ggcgacaggg
      661 caacacccac aatcagcatc gcttcgcgct tgccgttgat cagcgcatag agcttgtccc
      721 ccggctgcag gccacgcgcg acggcaaagc cttcggacac cagcacctcc agcgccccgt
      781 tgtgctgcgt ctccagcgca cgcccggccc gcagtgcgac ccggttgagg cgctgcggct
      841 cccccacgtc cagaccaatg aggcggccca cgatggggtc cgacacattg ggaatatcca
      901 ccggcacgat ctgcgtgatc gatgtttcca catgcgctgc gccgggaatg gcttcgagct
      961 ggcgctccag cgacagcggt gcccgcttga gcgtggcaaa cacgtcagca aagcgcgact
     1021 cggtgtagta caggtcacgc gaccaggaca gggcgtcgta ggccgtgaag ctggtgataa
     1081 atcccgtcac cccgctggcc accaccagtg cgatggtgat ggcctgactc cacatcaggc
     1141 gcagatcgcg cagcagtttg cggtcgaggg ccttcaccag gacagttcgc ttggttggag
     1201 cttgtgggcg ttggtttcca cccgctggat acgcccgccg cccagggaca ggatgcggtc
     1261 tgccatgccg gcaatggccg cgttgtgggt gatgacgatg gtggtggtgc ccagctcacg
     1321 gttgatgcgc tcgatgactt cgagcaccag cttgccggtg acatagtcca gcgcgccggt
     1381 gggctcatca cacaacagca cctcgggccg cttcacgatg gcccgtgcga tcgccacacg
     1441 ctgctgctcg ccgcccgaca gctgcgacgg aaagtggtcc agccggtcgg ccagccccac
     1501 catctccagc gcttcggtgg cgggcatggg attgacggcg atgtcggtga ccagctccac
     1561 gttttcgcgt accgtcaggc tcggaatcag gttgtagaac tggaacacaa agcccacgtg
     1621 ctcacgccgg tagcgcgtga gttctgcatc actggccgcg gagaggtcat ggtcggcaaa
     1681 gcgcgaaacg ccagtgctgg gccgatcgag cccgcccagg atgttgagca gtgtggactt
     1741 gccactaccc gaaggcccca acagcacgat gaactcaccg cggttgatgg tcaggtccac
     1801 atccaccagg gcgtgcactt gcacctcccc catctggtag gtcttggtca accgctcggc
     1861 aaaaaagaca gggacagcaa ccgctgggtc agcggcgccc gctttggtca ccgtgtcttg
     1921 catgggtgca tcactccatc agcatgccgg gtcgcgcatc tcggcgcgga tggcatcaat
     1981 ctgcttgagc acctcgggcg ccagcgtggt gccccaggcg ttgatgtctt cgtcgagctg
     2041 cgccaccgag gtcacgccaa tgatggtgct ggccacctgc cacttggtgt agcaaaacgc
     2101 cagggccatc tgcgaaggcg tcatgccgtt ctcacgggcc agcgtgttgt agcgcacggc
     2161 agcggcaaac gcctccttgc ggccccagcg ctgcttgcgt accgactcgt acttggcaat
     2221 gcgaccttcc ttcggtgcat cgggtccatc tacaccgctg acgtcgtatt tgccggtcaa
     2281 caggccaaaa gccagcggcg aataggccag cagagagacg cccaggtggt gcatggtttc
     2341 gtccagccca ttttccagcg cacggctgac caggttgtag acgttctgca ccgtggcgac
     2401 acgcggcagg ttgtgctgct cggccaggcg cacgaactcg tgcacaccgt agggcgtttc
     2461 attggacagg ccaatggcac gcaccttacc agccttgacc agggtgccca gcgcttcaag
     2521 ctgggcgtgc atgctggtca gggccgggtt gtcccggctc gggtcgtagt acatggcacc
     2581 gaacatgggc acgttgcggg tgggccagtg cagctggtac aggtcgatca catcggtttt
     2641 caggcgctgc aggctgccct cgcaggcagc gatgatgtcg gccgcggtca ggtcactgct
     2701 gccgccgcgc acccagggca tgccacgcga gggacccgcc accttggtgg caatcaccaa
     2761 tttctggcgc gcgccggggt tcttctccag ccagttgccg atgatttttt cggtggcatt
     2821 gaaagtctcg gccttgggcg gcaccgcgta catctcggcg gtatcgagga aattgacgcc
     2881 gcgctccagc gaatggctca gaatggtgtg ggccagcggc tcattcactt gttcaccaaa
     2941 ggtcatggtg cccaggcaga tggaggtgac ggagaggccg ctctggccca gggtgacttg
     3001 tttcatgttc aaaaggcttg tggtgggttt cagggtctat tttgcgccgt gtaaatgtca
     3061 caccgacatg tcacagcggc agcatcgggt tcaggtgccc cagatgggta ctgggcacca
     3121 gcgggtagtg atctgcgccc ggcagggcgt agtgccacca ttcgtgcgga atatgctcaa
     3181 aacccgcatc ttccatgaca gtcagcaggt gcatgcggtt ggcctgtgcc tcgggcgaca
     3241 ccttgtcact gaagtggtgt gacagcgccg tcatgtcgtc aaaggcggta cccatgtcca
     3301 actccagccc gccggcatcc accagcgccg cgcacatc
//
LOCUS       DAOSJM010000272         3333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_24960_length_3333_cov_5.541794, whole genome shotgun sequence.
ACCESSION   DAOSJM010000272 DAOSJM010000000
VERSION     DAOSJM010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24960_length_3333_cov_5.541794"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /gene="carB"
                     /locus_tag="PKX82_09625"
     CDS             complement(<1..1180)
                     /gene="carB"
                     /locus_tag="PKX82_09625"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HOF52554.1"
                     /translation="MPKRTDLKSILIIGAGPIIIGQACEFDYSGVQACKALREEGYKV
                     ILINSNPATIMTDPATADVTYIEPITWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     WHNGVLEKYKVELIGAKPEAIDKAEDRLKFKDAMTKIGLGSARSGIAHSMDEAWAVQK
                     TVGFPTVIRPSFTLGGTGGGIAYNPEEFETICKRGLEASPTNELLIEESLLGWKEYEM
                     EVVRDTADNCIIVCSIENLDPMGVHTGDSITVAPAQTLTDKEYQILRNASLAVLREIG
                     VDTGGSNVQFSINPKDGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGATPASFEPSIDYVVTKIPRFAFEKFPAADSRLTTQMKSVGEVMAMGRTFQ
                     E"
     gene            complement(1219..1887)
                     /gene="leuE"
                     /locus_tag="PKX82_09630"
     CDS             complement(1219..1887)
                     /gene="leuE"
                     /locus_tag="PKX82_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HOF52555.1"
                     /translation="MSFYGVTDIWTYLIGAIGIILLPGPNSLYVLSVATARGVRAGYQ
                     GAFGVFVGDTILLCLTALGAASLLRTHPALFMVVKYAGAAYLTWVGINLIRGAIAKWR
                     SGTPVDVDADAAVSQMVANLQQPFKRALVISLLNPKAILFLLSFFVQFIDPTYDTPAV
                     PFLILGGIIMGLSALYLSALIFAGARLAQGFSRRKRLSSSLSGAVGGLFMWFGAKLAT
                     ASLN"
     gene            complement(1884..3050)
                     /gene="carA"
                     /locus_tag="PKX82_09635"
     CDS             complement(1884..3050)
                     /gene="carA"
                     /locus_tag="PKX82_09635"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HOF52556.1"
                     /translation="MLLSLKGTTPPAILALADGTVYIGNSIGAAGSTVGEVVFNTSMT
                     GYQEILTDPSYCQQIVTLTYPHIGSYGINPEDVESGKVHAAGLIIKDLPLVASNFRST
                     QTLSQYLEAEGTVAIANIDTRQLTRQLRTQGAQNGCILALAAGETVTKEAIEKAVAAA
                     KGAPNMSGLDLAKVVSSTSSYGWTESEWVLGTGYGQQTTPKFHVVAYDFGVKKNILRM
                     LAERGCKVTVVPAQTPASEVLKHQPNGIFLSNGPGDPEPCDYAIAATKELIETGIPTF
                     GICLGHQIMALASGAKTFKMKFGHHGANHPVKDLDSGRVSITSQNHGFAVDERSLPAN
                     LRPTHISLFDNTLQGLERTDKPAFCFQGHPEASPGPHDIGYLFDRFIGLMEKRA"
     gene            complement(3202..>3333)
                     /locus_tag="PKX82_09640"
     CDS             complement(3202..>3333)
                     /locus_tag="PKX82_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011656070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoyl-CoA thioesterase"
                     /protein_id="HOF52557.1"
                     /translation="SLELEQQAWRGDTLLAEGRVRVGCVDGVTLRPRRIPKQVLDAL"
BASE COUNT          653 a          961 c         1044 g          675 t
ORIGIN      
        1 actcctggaa ggtgcggccc attgccataa cctcccccac cgacttcatc tgggtggtca
       61 ggcggctgtc ggcagcgggg aatttttcaa acgcaaaacg aggaatcttg gtgaccacgt
      121 agtcgatgct gggctcgaac gaagcggggg ttgcaccacc ggtaatgtcg ttgcgcaact
      181 cgtccagcgt aaagcccacg gccaatttgg ccgccacctt ggcaatcggg aaacccgtgg
      241 ccttggaagc cagcgcggag gaacgcgaca cgcgcgggtt catctcgatc accaccatgc
      301 ggccgtcttt gggattgatg gagaactgca cattggaacc accggtatcc acgccgattt
      361 cgcgaagcac agccaaagag gcgttacgca gaatctggta ttccttgtca gttagggtct
      421 gcgcaggggc cacggtaatg gagtcgccgg tgtgcacacc catgggatcc aggttctcga
      481 tggagcagac gatgatgcag ttgtcggcgg tgtcgcgcac cacttccatc tcgtactctt
      541 tccagccgag cagcgactct tcaatcagca actcgttggt gggcgaggct tccaggccgc
      601 gcttgcagat ggtctcgaat tcttccgggt tgtaggctat gccgccgccg gtgccgccca
      661 gtgtaaaact ggggcgaatg acggtgggga agcccacggt cttttgcacc gcccaggctt
      721 cgtccatgct gtgggcaatg ccggagcgtg cagaacccag accgatcttg gtcatggcgt
      781 ccttgaactt caggcggtct tcggctttgt cgatggcttc gggcttggcg ccgatcagct
      841 cgaccttgta tttttccagc acaccgttgt gccacaggtc cagcgcgcag ttcagcgcgg
      901 tctggccacc catggtcggc aggatggcgt cagggcgctc tttggcaatg atcttctcga
      961 cggtctgcca ggtgatgggc tcgatgtagg tcacgtccgc cgtggcgggg tcggtcatga
     1021 tcgtggcggg gttgctgttg atcaggatga ccttgtagcc ctcttcccgc agggccttac
     1081 aggcctgtac gccggagtag tcgaactcgc aggcttgccc aatgatgatg gggccagcgc
     1141 cgatgatgag gatgcttttg aggtcggtac gttttggcat ttttggtgct ctttactttt
     1201 caatgaattc aggttttatt agttcaaact ggcggtggcc agcttggccc caaaccacat
     1261 aaacaggccg cccacagcgc cagagagact gctggacagg cgcttgcgcc ggctaaagcc
     1321 ttgcgccagg cgggcccccg caaaaatcag ggcagacagg tacagggcac tcagacccat
     1381 gatgattccg cccaggatca ggaaaggcac tgcgggcgtg tcgtaggtag ggtcgatgaa
     1441 ctgcacaaag aacgacagca gaaacagaat cgccttcggg ttcagcagac tgatgaccag
     1501 cgcgcgcttg aacggctgct gcaggttggc caccatctgc gacacggctg catccgcatc
     1561 cacgtctacc ggcgtgcccg agcgccactt ggcaatggca ccgcgaatca ggttgatgcc
     1621 tacccacgtc agatacgctg cacccgcgta cttcaccacc atgaacaaag cgggatgcgt
     1681 acgcagcaaa ctcgcagcgc ccagcgccgt gagacacagc aaaatagtgt cccccacaaa
     1741 cacgccaaac gcgccctggt agccggcgcg cacgccgcgt gcggtcgcta ccgacaggac
     1801 atacagcgag ttcgggccgg gcagcaaaat aatgccgatc gcaccgatca ggtaggtcca
     1861 gatatcggta acgccgtaga agctcatgcg cgcttttcca taagaccgat gaaacggtca
     1921 aacaagtagc cgatgtcgtg cgggccgggg gaagcttccg ggtgaccctg gaagcagaac
     1981 gcgggcttgt cggtacgctc caggccctgc agcgtgttgt caaacaggct gatgtgcgtg
     2041 gggcgcaggt tggcgggcag gctgcgctca tccaccgcaa aaccatggtt ctggctggtg
     2101 atggacacgc gaccgctgtc gaggtcttta accggatggt tggcgccgtg gtgaccaaat
     2161 ttcatcttga aggtcttggc acccgaggcc agggccatga tctggtggcc cagacagata
     2221 ccgaaggtgg gaatgccggt ttcgatcagt tctttggtcg cagcaatggc atagtcgcag
     2281 ggctccgggt cacccgggcc attggacagg aagatgccgt tgggctgatg cttgagcacc
     2341 tcagaggcag gcgtttgcgc cggcaccacg gtgaccttgc agccccgctc ggccagcatg
     2401 cgcaggatgt ttttcttgac gccaaagtcg taggccacca catggaactt gggtgtggtc
     2461 tgctggccat agcctgtgcc aaggacccac tcggactcgg tccagccgta gctgctggtg
     2521 ctggagacca ctttggccag gtccaggcca gacatgttgg gtgcgccctt ggcggcggca
     2581 acagccttct caatggcttc cttggtgaca gtttcaccag cggccagcgc cagaatgcag
     2641 ccgttctgcg cgccctgggt gcgcagctgg cgggtcaact gacgcgtgtc gatgttggca
     2701 atggcgaccg tgccttcagc ctccagatac tgcgagagtg tctgtgtaga gcggaagttg
     2761 gacgccacca aaggcaggtc cttgatgatc aggccggcgg cgtggacctt gccggactcc
     2821 acgtcttcgg ggttgatgcc gtagctgccg atgtggggat aggtcaacgt gacgatctgc
     2881 tggcaatagc tgggatcggt gaggatctcc tggtagccgg tcatggacgt gttgaacacg
     2941 acctcaccga ctgtggagcc ggcggctcca atggaattgc caatgtagac cgtgccgtct
     3001 gcgagcgcca ggatggcggg tggggtagtt cccttgagag acaaaagcac tggtttctcc
     3061 gaatgggtta cgggtacgcc caaacgcgct caagaaggac tcctggcgga tgcttttacg
     3121 agggaatgtt gcttaaactg cgcttgggac tgctgtttac tggaaagcct cccattatag
     3181 cggagggctt cgcgttttcc cttacagggc gtccaaaact tgctttggga tgcgccgcgg
     3241 ccgcaaggtc acgccatcca cgcagcccac ccgcaccctg ccctccgcca gcaaagtgtc
     3301 gcctcgccaa gcctgctgtt ccagttccag gga
//
LOCUS       DAOSJM010000273         3326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25019_length_3326_cov_4.435341, whole genome shotgun sequence.
ACCESSION   DAOSJM010000273 DAOSJM010000000
VERSION     DAOSJM010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25019_length_3326_cov_4.435341"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /gene="pgk"
                     /locus_tag="PKX82_09645"
     CDS             complement(<1..255)
                     /gene="pgk"
                     /locus_tag="PKX82_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOF52558.1"
                     /translation="MQVIRFSDLVAQGKVADKRVFIRADLNVPQDDAGNITEDTRIRA
                     SIPCIEMALKAGAAVMVTSHLGRPTEGDFKPEDSLAPVAKR"
     gene            complement(320..667)
                     /locus_tag="PKX82_09650"
     CDS             complement(320..667)
                     /locus_tag="PKX82_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOF52559.1"
                     /translation="MSPTDGWTLGVLVGMGVVTVVTRCFFFMSNRSWQLPHWAQRGLQ
                     YAPIAALSAVVLPEVVMTQGQLITTWQDARLFAAVAGAAYFFVRKGQGQAVLGTIVSG
                     MVVYLPLHIGLGW"
     gene            complement(664..1407)
                     /locus_tag="PKX82_09655"
     CDS             complement(664..1407)
                     /locus_tag="PKX82_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOF52560.1"
                     /translation="MQRWRAWWRHPEFQLGLREMAAVSPGLAAWGLMTGVTMVKSGMS
                     TFEAVAMSLLVFAGSSQLAAIPLIAAGAPVWVIWATAFCVNLRFVVFSAHMRPYLMHL
                     PLRWRLFNGYLTADLSYVMFTKRFHHPATDVGGQRDQLAYLGGGSFFNWTSWQAASLL
                     GIALANTIPTQWGLGFAGILALLGVGCSLASSTLRRVSAGVAGMAAVAAFALPFKLNI
                     VVAIAAAVALCLMLEQLQPPAPPLAKGKA"
     gene            complement(1407..2396)
                     /gene="fmt"
                     /locus_tag="PKX82_09660"
     CDS             complement(1407..2396)
                     /gene="fmt"
                     /locus_tag="PKX82_09660"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOF52561.1"
                     /translation="MQPLRVIFAGTPEFARLALERLHAAGHTIALVLTQPDRPAGRGM
                     KLQASAVKQFALEHTIPVAQPRSLRLDGKYPDDAVAARKAIAEADADVMVVAAYGLIL
                     PQWVLDDMVAPHADGRGRYGCLNIHGSLLPRWRGAAPIHRAIEAGDSHTGVTIMQMDE
                     GLDTGDMLLKESLPILEADTTATLHDKVAELGARMVVQALDFAAAGQLQPVKQPADGI
                     TYAHKIEKHEAAIDWQQDAETIVRRVRAFNPFPGASTVLDGESIKVWSASVQAGSPQA
                     VFGTILAVAPEGIAVAAMNSIVILKELQRAGSKRMPVADFVRGFPLQAGQVLA"
     gene            complement(2397..2906)
                     /gene="def"
                     /locus_tag="PKX82_09665"
     CDS             complement(2397..2906)
                     /gene="def"
                     /locus_tag="PKX82_09665"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOF52562.1"
                     /translation="MALLPILCYPDPKLHKVAKPVAVVDERVQTLVRDMLETMYEAKG
                     IGLAATQIDVHERVIVIDVSEERDQPLVLINPEIVWTSPTKHLNEEGCLSVPGIYDGV
                     ERFDAVHVKALDGQGQSRTIEAVELLAVCIQHEMDHLMGKVFVEYLSPLKRNRIKTKM
                     LKALREESR"
     gene            3067..>3326
                     /locus_tag="PKX82_09670"
     CDS             3067..>3326
                     /locus_tag="PKX82_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOF52563.1"
                     /translation="MAKSKLALAALTSLASALTVGPALAQQYPVTSAQKATANEVAAK
                     GVPLSELAPNAPETYTIKRGDTLWDISKLYLKSPWRWPELWG"
BASE COUNT          699 a         1061 c          956 g          610 t
ORIGIN      
        1 gcgcttggcc acgggggcca gggagtcttc gggtttgaag tcgccttcgg tagggcgacc
       61 caagtgcgag gtcaccatga ccgccgcacc ggccttcagc gccatttcaa tgcagggaat
      121 actggcgcgg atgcgggtgt cttcggtgat gtttccggcg tcatcctgcg gaacattgag
      181 atcggcacgg atgaaaacgc gtttgtcggc aactttgcct tgggcaacca ggtcagagaa
      241 acggatcact tgcatgaaaa aacctcaggt agttgaaata ggcccgcagt gcggtcacac
      301 cacgcattgt aaaagtgcgc taccagccca ggccgatatg cagcggcagg tacaccacca
      361 tccctgaaac aatggtgccc agcaccgcct gaccttgccc cttgcggaca aaaaaatagg
      421 ccgctcccgc aaccgcagca aacaggcgcg catcctgcca ggtcgtaatg agttgcccct
      481 gggtcatgac cacctcgggc agtaccaccg ccgacagtgc ggcaatcggc gcgtactgca
      541 agccgcgctg ggcccagtgg ggaagctgcc aggaccgatt gctcatgaag aagaagcaac
      601 gcgtcaccac cgtcaccaca cccatcccca cgagcacccc cagcgtccag ccgtccgtag
      661 gactcatgct ttgcctttgg caagaggagg tgctggcggc tgcagttgct ccagcatcag
      721 gcacagcgcc acggctgccg caatagccac cacaatattg agcttgaagg gcaaggcaaa
      781 cgccgccacg gcggccatgc ccgccacccc agcggacacc cgccgcaagg tgctactagc
      841 cagcgagcaa ccgaccccca gcagcgccag aatgccggca aaccccaagc cccattgtgt
      901 tgggatggtg ttggccagcg caatacccaa caggctggca gcctgccagc tggtccagtt
      961 gaaaaaactg ccaccgccca ggtaagccag ctggtcgcgc tggccaccga catccgtcgc
     1021 gggatggtgg aagcgtttgg taaacatcac gtagctgagg tcggctgtca agtagccatt
     1081 gaacagccgc cagcgcagcg gcaggtgcat caggtacggt cgcatgtggg cactgaacac
     1141 cacaaaacgc aagttgacgc aaaaggcagt ggcccagatc acccagactg gcgcacctgc
     1201 ggcgatcaag ggtatggcag ccagctgcga actgccggca aacaccagca ggctcatggc
     1261 cacggcctcg aacgtgctca tgccggactt gaccatggtc acgccggtca tcagccccca
     1321 ggctgccaaa cctggcgata cggccgccat ctcgcgcagc cccaattgaa actcggggtg
     1381 gcgccaccag gcccgccagc gctgcatcac gccagcacct ggcctgcctg caacggaaag
     1441 ccgcggacaa agtccgccac gggcatgcgc ttgctgccgg cccgctggag ctctttcaaa
     1501 attactatcg aattcatagc ggccaccgca ataccctcgg gggctacagc cagaattgtt
     1561 ccaaaaacag cctgcggaga ccccgcttgc accgatgcac tccagacctt gatggattcg
     1621 ccatccagca ccgtcgaggc acccggaaag gggttgaacg cccgcaccct gcgcacgatt
     1681 gtttcggcat cctgttgcca gtcgatcgcc gcctcatgct tctcaatttt gtgggcgtac
     1741 gtgattccgt ctgctggttg tttgacgggc tgtaactggc cagcagctgc gaaatccagt
     1801 gcctgaacga ccatgcgcgc gccaagctca gccaccttgt cgtgcagggt ggcggtggtg
     1861 tcggcttcca gaattggcag gctctccttg agcagcatgt cgcccgtgtc cagcccctcg
     1921 tccatctgca tgatggtgac accggtatgg ctgtcgcccg cttcaatcgc gcgatggatg
     1981 ggcgctgcgc cacgccagcg gggcagcagg gagccgtgga tgttcaggca gccataacgc
     2041 ccacggccat cggcgtgcgg ggcgaccata tcgtccagca cccactgcgg caggatcagc
     2101 ccataggcag cgaccaccat cacatccgca tcggcctccg caatcgcctt gcgggcggct
     2161 actgcgtcat ctggatactt tccgtccagt cgcaggctgc gcggctgtgc cactggaatg
     2221 gtgtgctcca gcgcaaactg cttgaccgca gaggcctgca gtttcatgcc ccgccccgcg
     2281 ggccggtcgg gctgggtcaa caccagggca atggtgtgtc cagcagcatg caatcgctca
     2341 agagccagtc gggcgaattc cggtgtgccg gcgaaaatga ctcttagagg ttgcacttaa
     2401 cgcgattcct cacgcagggc tttgagcatt ttggttttga tgcggttgcg cttcaggggg
     2461 gaaaggtatt ccacaaagac cttgcccatc agatggtcca tctcgtgctg gatgcacact
     2521 gcaagcagtt cgacggcctc gatggtccgg ctctggccct ggccatccag cgccttcaca
     2581 tgcactgcgt cgaaacgttc cacgccgtca taaatcccag gcaccgagag gcagccctct
     2641 tcattcagat gcttggtcgg actggtccag acgatttccg ggttgatcag taccagcggc
     2701 tggtcacgct cttcggatac gtcgatcacg atcacccgct catggacgtc aatctgggtt
     2761 gctgccaggc caatcccttt ggcttcatac atggtttcca gcatgtcgcg caccagcgtc
     2821 tggacgcgct cgtccactac ggccacgggc tttgccactt tgtgcagttt ggggtcggga
     2881 tagcaaagaa tcggtagtaa tgccatggaa agctcttcaa agatgtggct attttcgcca
     2941 cttttggcgg ctctggtaca gcctgttacc caaaaacgtt gccgaatcaa gggcttgagc
     3001 gcaaaatcgc gcgtaaatta tcaagttatg ccaacccgac tgcactccga tatgacgaca
     3061 cacttcatgg cgaaatcaaa attggcactg gccgcactga ccagtctggc aagtgccttg
     3121 acggtaggcc ccgccctggc acagcagtat cctgtcacgt cagcccaaaa ggccacagcc
     3181 aacgaggtgg ccgccaaagg cgtgccgctg agcgaactgg ccccgaatgc gcctgagacg
     3241 tacaccatca agcgtggcga tacgctgtgg gacatttcca agctctacct caagagcccc
     3301 tggcgctggc ctgagttgtg gggcat
//
LOCUS       DAOSJM010000274         3325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25037_length_3325_cov_4.888991, whole genome shotgun sequence.
ACCESSION   DAOSJM010000274 DAOSJM010000000
VERSION     DAOSJM010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25037_length_3325_cov_4.888991"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /gene="der"
                     /locus_tag="PKX82_09675"
     CDS             <1..998
                     /gene="der"
                     /locus_tag="PKX82_09675"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOF52564.1"
                     /translation="VANKAEGMTQGVQLGEFFELGLGEVFPVSAAHGQGIRSLVEVSL
                     DPLNLPDVDDEGAEQDPTVIKLAVAGRPNVGKSTLINTWLGEERLVAFDMPGTTRDAI
                     SVPFERNGQKFELVDTAGLRRKGKVFEAIEKFSVVKTLQAIESASVVLLLIDATQGVT
                     DQDAHIAGYILESGRAVVIAVNKWDAVDEYQRELVKRSMETRMGFLKFAAIHWISAQK
                     RQGLGPVWDSIAQAHRAANCKMPTPVLTRLLLEAVQFQTPKKTGMYRPKLRYAHQGGM
                     NPPIIVVHGNSLEHVTEAYKRFLEGRFRKEFDLVGTPLRIEMKTSQNPYSDKDDD"
     gene            1038..1304
                     /gene="hfq"
                     /locus_tag="PKX82_09680"
     CDS             1038..1304
                     /gene="hfq"
                     /locus_tag="PKX82_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOF52565.1"
                     /translation="MNTENIVNNKGQLLQDPFLNALRKEHVPVSIYLVNGIKLQGQIE
                     SFDQYVVLLRNTVTQMVYKHAISTIVPGRAVSFAVNGAEDAPAA"
     gene            1360..2532
                     /gene="hflX"
                     /locus_tag="PKX82_09685"
     CDS             1360..2532
                     /gene="hflX"
                     /locus_tag="PKX82_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOF52566.1"
                     /translation="MTAIPKLPDAPTILVGVDFGLPNFDGELEELGLLAQTAGMTPVA
                     RITCKRRAPDAALFVGSGKADEIKLLAQQTGATEILFDQALSPGQQRNLERHLELPVN
                     DRTFLILEIFAQRARSHEGKLQVELARLQYLSTRLVRRWSHLERQTGGAGVRGGPGEK
                     QIELDRRMISETIKRTKERLAKVKRQRQTQRRQRERRDAFNISLIGYTNAGKSTLFNA
                     LVKARAYAADQLFATLDTTTRQLYLGEAGRSVSLSDTVGFIRDLPHGLVDAFQATLQE
                     AIDADLLLHVVDASNPEFVEQIAQVQSVLKEIGAADIPQLLVFNKLDALEPERRPVRM
                     SDEFEIDGIQTPRVFVSARTAEGLPLLRQRLSSIVSASGEPAADPVDSPENNGAGQ"
     gene            2571..>3325
                     /locus_tag="PKX82_09690"
     CDS             2571..>3325
                     /locus_tag="PKX82_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflK"
                     /protein_id="HOF52567.1"
                     /translation="MKLPMYAVKQPSWLQRLLGVFNLNDSRWGRGDDKPSDAPKQDGA
                     ADDNEQPVRPPPENAPQGGRKGANAGPPDLDELWRDFNRKLGGLFGGSQPPRGNAGGG
                     GNSGGFQPDMKSAGLGAGLILAVVALIWLGTGFFIVQEGQQAVITQFGKYKSTVNAGF
                     NWRLPYPIQRHELVFVTQIRSVDVGRDTVLKATGLKESAMLTEDENILDIKFAVQYRL
                     SDARAFLFESKNPSDAVVQAAETAVREVLGKMK"
BASE COUNT          718 a          884 c         1017 g          706 t
ORIGIN      
        1 tggtggccaa caaggctgaa ggtatgaccc aaggcgtgca actgggcgaa ttctttgagc
       61 ttggccttgg cgaagtgttt ccggtctctg ccgcgcatgg acagggtatt cgctcgttgg
      121 tggaggtctc gctcgatccg ctaaatctgc ccgacgttga cgatgaaggc gcagagcagg
      181 acccaaccgt catcaaactc gccgtggcag gccgtcccaa tgtaggtaag tccaccctga
      241 tcaacacctg gctgggtgaa gagcggctgg tcgcctttga catgccaggg actacacggg
      301 atgccatttc ggttccgttt gaacgcaatg gtcagaagtt tgaattggtg gataccgctg
      361 gattgcgccg caagggcaaa gtgttcgaag cgattgagaa gttttcggtc gtcaagacac
      421 tgcaggctat cgagtctgcc agtgttgttc tgcttctgat cgatgctaca cagggtgtga
      481 cagaccagga tgcgcatatc gctgggtaca tcctggagtc cggccgtgcg gtggtgattg
      541 ccgtcaacaa atgggatgca gtggacgaat accagcgcga actggtcaag cggtcgatgg
      601 aaacccgcat gggattcctg aagtttgcag ccattcactg gatttctgcc cagaagcgac
      661 aaggcttggg ccctgtgtgg gactcgattg cgcaagccca tcgtgcggcg aactgcaaga
      721 tgccgacgcc cgtgctgacc cgcttgctac ttgaagcggt ccagttccag acgcccaaga
      781 aaaccggcat gtaccgcccc aagctgcgct acgcacacca ggggggaatg aatcctccca
      841 tcatcgtggt gcatggtaac tcgctggaac atgtcaccga agcctacaaa cgatttctgg
      901 aaggacgttt ccgcaaggaa ttcgatctgg tgggtacgcc cttgcgtatc gaaatgaaga
      961 cctcacaaaa cccctattcg gacaaggacg acgactgact gcaggtgggt gtggtatcgt
     1021 ctcttcttct taaattcctg aacacggaga atatcgtgaa caacaaaggg cagctcttgc
     1081 aagacccatt cctcaacgcg ttgcggaagg aacatgtacc ggtttccatt tatctggtca
     1141 acggcatcaa acttcaaggc cagatcgaat catttgatca gtacgtcgtg ttgttgcgca
     1201 ataccgtgac ccagatggtc tacaaacatg ccatttccac catcgtcccc ggacgtgcgg
     1261 tgagtttcgc tgtgaacggt gctgaagacg ctcctgcggc ttgagtaatg gccgcaattg
     1321 cggcagcatt tctgaaaacc tggttttcat ttccctgatt tgactgcaat acccaaactc
     1381 cctgatgccc cgacgattct ggtgggcgtc gattttggct tgcccaattt cgacggtgaa
     1441 ttggaggaat tggggttgct ggcccagact gcaggcatga ctccggttgc ccggatcacc
     1501 tgcaagcgcc gtgcgccgga tgccgcattg tttgtcgggt ctggcaaggc cgatgagatc
     1561 aagttgctgg cacagcagac cggtgccacg gaaatcctgt tcgaccaggc gctgagcccg
     1621 gggcagcagc gcaatctgga acggcatctc gaactgccgg tcaacgaccg tactttcctg
     1681 attctggaga tattcgccca gcgtgcccga agccacgaag gcaaactgca ggtcgaactt
     1741 gcacgcctgc agtacctgag tacgcgtctg gtgcggcgat ggtctcacct ggagcgtcag
     1801 acggggggcg ccggggtccg tggcggcccg ggtgagaagc agatcgaatt ggatcgccgg
     1861 atgatcagcg agacgatcaa gcggaccaag gaacgcctgg ccaaggtcaa gcgccagcgg
     1921 caaacccagc ggcggcagcg cgagcggcgg gatgctttca acatctccct gatagggtac
     1981 accaacgcgg gcaaatccac gctgttcaac gccctggtca aagcccgagc ctacgcggcc
     2041 gaccagctgt ttgccacgct ggataccact acgcgccagc tttatctggg tgaggcaggg
     2101 cgatcggtat cactgtcgga tacagtgggt tttatccgtg atctgcccca tggtctggtg
     2161 gatgcgttcc aggcgacctt gcaggaggcc atcgatgccg atctgctgtt gcatgtggtg
     2221 gatgcatcca atccggagtt tgtggagcag attgcccagg tgcagagcgt gttgaaggaa
     2281 atcggtgccg cggatattcc acagttgctg gtattcaaca aactcgacgc cttggagccg
     2341 gagcgtcggc ccgtgagaat gtccgatgag tttgagattg atggtatcca gacgccccgg
     2401 gtatttgtca gcgcccgcac cgcggaaggt ttgccgctgt tgcgtcagcg gctctcttcc
     2461 atcgtgagcg caagtggtga accagcagct gatccggtcg attcccctga aaacaatggg
     2521 gccggccagt gattgggcac aatcagtgca ttgaggatcg agataaagat atgaaattac
     2581 ccatgtacgc agtcaagcaa ccgtcctggt tgcagcgact gcttggcgtt tttaacctga
     2641 atgactcgcg ttggggccgt ggtgacgaca aaccgtccga cgccccgaag caggatggtg
     2701 cggcggacga caacgagcag cccgtgaggc cacctccaga aaatgcgcca caaggcgggc
     2761 gcaaaggcgc caatgccggt ccaccggacc tggatgagtt gtggcgtgat ttcaaccgca
     2821 agctgggcgg gctgtttggt ggctcacagc caccgcgagg caatgctggc ggtggtggta
     2881 actctggcgg ctttcagccc gacatgaaga gtgcgggctt gggtgcaggg ttgatcctgg
     2941 cggtggttgc attgatctgg ctggggaccg gcttcttcat tgttcaggaa ggacagcaag
     3001 ctgtcattac ccagttcggc aagtacaagt ccacggtcaa tgccggtttc aactggagat
     3061 tgccgtaccc gatccagcgg catgagctgg ttttcgtcac acagattcgt tctgtggacg
     3121 taggtcgcga cacggtgctg aaagccacgg ggctgaagga atcagcaatg ttgaccgaag
     3181 acgaaaacat tctcgatatc aagttcgcgg tgcaataccg tttgagcgat gcgcgggctt
     3241 tcctgtttga aagcaagaac ccgtcggatg cggtggtcca ggccgccgag actgcggtac
     3301 gcgaagtgct gggcaagatg aagat
//
LOCUS       DAOSJM010000275         3304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25256_length_3304_cov_3.045860, whole genome shotgun sequence.
ACCESSION   DAOSJM010000275 DAOSJM010000000
VERSION     DAOSJM010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25256_length_3304_cov_3.045860"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PKX82_09695"
     CDS             <1..880
                     /locus_tag="PKX82_09695"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HOF52568.1"
                     /translation="TTVFDALGVGEADTESLRGSTWDGVLNTLDTCTAQGLLRADHHI
                     HVRCELPAPNTVELFEPFHAHPRLSLISLMDHTPGQRQWEDIEQARTYYTGKKGWSQE
                     KFERQVAHAHVLQAEYARPHRQYFLDYCRAHGIALASHDDTTVAHVQQAHEEGASMSE
                     FPTTVAAAQEARARGLRTVMGGPNVVRGGSHSGNVAAAELGRLGLLDILSSDYVPGSM
                     LSGAMRLVNDGIMSLPQAVATVTRTPALATGLDDRGALSTGLRADLIQVRMATLPDGT
                     QHPVVRAAWREGQRVL"
     gene            883..1677
                     /locus_tag="PKX82_09700"
     CDS             883..1677
                     /locus_tag="PKX82_09700"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOF52569.1"
                     /translation="MSVNMVPVSGGRLRVVTLNTWKCEGAYALRLQAMVEQLQPLHAD
                     VLVLQESFASADGAVSTARSLAQALHLEYAWIPDRPKLRLCDGQWMESHSGLAVLTRW
                     PILENRNIVLPSSEADGGRSAQLCRLVVPGGPLWLANVHLTHLANGATLRHQQLAHLL
                     QSTATLTMGDPLLVCGDFNVPLEDSEMWVFLGRPWGLSDTWPAMNGPKVTHITEQGVP
                     VNLDHVLQRTTTAWPVLKTEVVLQQASTRLGLCASDHFGVLVDLAS"
     gene            1756..2616
                     /gene="phnC"
                     /locus_tag="PKX82_09705"
     CDS             1756..2616
                     /gene="phnC"
                     /locus_tag="PKX82_09705"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOF52570.1"
                     /translation="METALRVHKLNKHFANGKHALNDINLTVRKGEMVALIGASGSGK
                     STLMRHMAGLIAADGQSDSLIEIDGSCVQQGGRVHRDIRRVRAKVGFVFQQFNLVDRL
                     PVMVNVLVGMLHRMPWWRSLFRVFSTHERAQALEALQRVGIAECFAQRASTLSGGQQQ
                     RAAIARALVQGAKVVLADEPIASLDPESSRKVMEILSRINKEDQCTVIVSLHQVDMAL
                     KYCPRVIALHQGRVVFDGASSLLTPPMLRELYGVHADEILADSQRPVPAPAHVPVDEP
                     SWSVPVAQAA"
     gene            2646..>3304
                     /gene="phnD"
                     /locus_tag="PKX82_09710"
     CDS             2646..>3304
                     /gene="phnD"
                     /locus_tag="PKX82_09710"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF52571.1"
                     /translation="MFKKLVATSVATLALGLSGTTVFAQDLNFGIIATESSQQLKQQW
                     QPLLDELNRQTGMKVNAFFAPDYAGVIEGMRFNKVHFAWFGNKSAMEAVDRANGEVFA
                     KVVNVDGTEGYYSHLIVHRDSPIKTLDDVLKNGKNLNFGNGDPNSTSGFLVPSYYVFA
                     KNGVDAKTHFKLTRSANHETNALAVANKQVDVATNNSETLAKLDKTLPEKIKDIRVIW
                     TS"
BASE COUNT          634 a         1015 c         1027 g          628 t
ORIGIN      
        1 caccacggtg tttgacgcgc tgggggtggg ggaggcggac accgagagtc tgcgcggcag
       61 tacctgggat ggcgtgctga acacgctgga tacctgtacc gcgcaagggc ttctgcgtgc
      121 cgaccaccac atccatgtgc gctgcgaatt gccagcaccc aacacggtgg agctgttcga
      181 gccgtttcac gcgcacccgc gcctgtcgct gatctccctg atggaccaca ccccggggca
      241 gcgccagtgg gaggacattg agcaggcccg tacctattac accggcaaga agggctggag
      301 ccaggagaag ttcgagcgcc aggtggccca tgcccatgtg ctgcaggcgg aatatgcccg
      361 gccccatcgg cagtattttt tggactactg tcgcgcgcac ggcattgcgc tggccagtca
      421 tgacgacacc acggtagcgc acgttcagca ggcccatgaa gagggcgcca gcatgtccga
      481 gtttcccact accgtggcgg cagcgcagga agcgcgcgcc cgtggcctgc gcacggtgat
      541 gggcgggccc aacgtggtgc gcggtggttc ccactccggc aatgtggccg cggctgaact
      601 aggccgtctg ggactgctcg acatcctgtc atccgactac gtgcctggca gcatgctcag
      661 tggcgccatg cggctggtga atgacggcat catgagcctg ccacaggcgg tagccaccgt
      721 gacgcgtacg cctgccctgg ccaccggtct ggacgaccgt ggtgcgctct caacgggttt
      781 gcgtgccgac ctgattcagg tgcgcatggc cactttgccg gatggtacgc agcacccggt
      841 ggtgcgtgcc gcctggcgcg aaggccagcg ggttctctga cgatgtctgt gaacatggtg
      901 ccagtctctg gtgggcgcct gcgggtggtg acactcaata cctggaagtg tgagggagct
      961 tacgccctgc gcctgcaggc catggtggaa cagttgcagc cgctccatgc cgatgtgctg
     1021 gtgttgcagg aatcatttgc cagtgcggat ggggcggtca gcaccgcccg gtcactggcc
     1081 caggccctgc acctggaata tgcatggata ccggaccgcc ccaagttgcg gctgtgtgac
     1141 ggacagtgga tggagagcca ttccggcctg gccgtgctca cgcgctggcc cattctggag
     1201 aaccggaaca tcgtcttgcc gtccagcgaa gccgatggcg ggcgctctgc gcagctatgc
     1261 cgcctggttg ttccaggcgg gccgctgtgg ctggccaatg tgcatctgac ccacctggcc
     1321 aacggtgcca cactgcgcca ccagcaactg gcgcacctgt tgcaaagcac ggcaacgctg
     1381 acgatgggag atcctctgct ggtgtgcgga gacttcaacg tgccgctgga agactccgaa
     1441 atgtgggtgt tcctgggccg cccctggggg ctcagtgaca cctggcctgc catgaacggc
     1501 ccgaaggtga cccacatcac ggagcagggc gtgccggtga acctggacca tgtgctgcag
     1561 cggacaacga ccgcctggcc ggtgctgaaa accgaagtcg tcctgcagca ggccagcacc
     1621 cgcctggggc tgtgcgccag cgaccatttt ggcgtgctgg tggacctggc gtcataaagg
     1681 cttccccgga ttttcatcaa accgtcaccc tggtcgcaga gactaaaacc tgtctagaca
     1741 aatcacgaga cccagatgga aacagcactg cgcgtacaca agctcaacaa gcattttgcc
     1801 aatggaaagc acgccctgaa cgacatcaat ctgaccgtcc gcaagggtga gatggtggct
     1861 ttgatcggcg cctctggatc cggcaagtcc acgctgatgc gccacatggc ggggttgatt
     1921 gcagcagacg ggcagtcgga ctccctgatc gaaattgacg gctcctgtgt gcaacagggt
     1981 gggcgcgtgc accgcgacat ccgtcgggtg cgtgccaagg tgggctttgt ttttcagcag
     2041 ttcaatctgg tggaccggtt gccggtgatg gtgaatgtgc tggttggcat gctgcaccgc
     2101 atgccctggt ggcgcagcct gtttcgcgtg ttcagcacgc acgaacgtgc gcaggcactg
     2161 gaggcgctgc aacgtgtggg cattgccgaa tgctttgccc agcgtgcatc gaccctgtcg
     2221 ggcggtcagc agcaacgggc tgccattgcg cgcgctctgg tgcagggcgc caaagtggtg
     2281 ctggcggatg agcctattgc gtccctggac cccgagtctt cccgcaaggt catggaaatc
     2341 ctgtcacgta tcaacaagga agaccaatgc actgtgatcg tatcgctgca ccaggtcgac
     2401 atggcgctga aatactgccc ccgtgtgatt gccctgcacc aggggcgggt ggtgtttgat
     2461 ggtgcttcca gcctgttgac accccccatg ctgcgcgagc tgtatggcgt gcatgccgac
     2521 gaaattctgg cggactccca acgccctgta cctgcaccgg cccatgtgcc ggtggacgag
     2581 ccctcttggt ctgtgcctgt ggcacaagct gcctgatttc cccctttacc tctacaggag
     2641 ttttcatgtt taaaaagcta gttgcgacct cggtcgccac cctggcgctt ggcctgtccg
     2701 gcaccaccgt gtttgcccag gacctcaatt tcggcatcat cgccacggaa tcttcgcagc
     2761 aactcaaaca gcagtggcaa cccttgctgg acgagttgaa ccgccagacc ggcatgaagg
     2821 tcaacgcatt cttcgcgccc gactatgccg gcgtgattga aggcatgcgt ttcaacaagg
     2881 tccattttgc gtggttcgga aacaagtccg ccatggaagc ggtagaccgt gccaacggtg
     2941 aagtgtttgc caaagtggtc aatgtggatg gcaccgaagg ctactactcc cacctgatcg
     3001 tgcaccgcga cagccctatc aagaccctgg acgatgtact caagaacggc aagaacctga
     3061 actttggcaa tggcgacccc aactccacct ccggcttcct ggtacccagc tactacgtgt
     3121 ttgccaagaa cggcgtcgat gccaagaccc acttcaagct gacccgctcg gccaaccacg
     3181 aaaccaacgc cctggcagtc gccaacaagc aggtggatgt ggccaccaac aacagcgaaa
     3241 ccctggccaa gctggacaag accctgccag agaaaatcaa ggacatccgt gtcatctgga
     3301 cgtc
//
LOCUS       DAOSJM010000276         3303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25260_length_3303_cov_4.356835, whole genome shotgun sequence.
ACCESSION   DAOSJM010000276 DAOSJM010000000
VERSION     DAOSJM010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25260_length_3303_cov_4.356835"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="PKX82_09715"
     CDS             <1..659
                     /locus_tag="PKX82_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52572.1"
                     /translation="VIVTHYHADHIYGLQAFKAKGARIIAHQAAREYLNSDTARLRLI
                     ASRQELAPWIDESTRLVPADEWITGPSELVVGGVRLVLQPVGPSHTPEDIVVYLPEQK
                     VLFAGDLVFRSRIPFVGQADSGHWIKALDTLLAFDAKVIVPGHGPLSSEARKDMELTR
                     DYLAYLRSSMGQAARNMEPFEDAYKAADWMKFEHLPLFRVANRMNAYNTYLLMEKEAP
                     "
     gene            656..1147
                     /locus_tag="PKX82_09720"
     CDS             656..1147
                     /locus_tag="PKX82_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52573.1"
                     /translation="MNVNLDGVRRRSLLAIGGGVAACLAVPSAWSASSALPYPQSLAD
                     ELARALKVGQPLVVMVSLDGCPFCRVARDSYLAPMMRQEGVPVVQIDMRTAVAVRDFR
                     QFAMTHDALARAWGIKVAPTVLFFGPGGQEVAERLVGGYIPDFYGAYLDQRMQTARAA
                     IKP"
     gene            1144..1368
                     /locus_tag="PKX82_09725"
     CDS             1144..1368
                     /locus_tag="PKX82_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52574.1"
                     /translation="MIRRIPVLWLAVVAGWLAIAPLVPEPHLVEKTRMLLSGTLVRPI
                     DIFDLLLHSVPLMLLAWRLVVMAQDSRDKP"
     gene            1379..1993
                     /locus_tag="PKX82_09730"
     CDS             1379..1993
                     /locus_tag="PKX82_09730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF52575.1"
                     /translation="MHSPLNFWDQRFAEPGFKYGTEPNAFLREQAVRLPPAGRVLVPG
                     DGEGRNGVWLAAQGHQVASVDSSAVGLQKARDLASQHGVELETVVADLAVWEPAPASV
                     DGVVLIYTHLPGSIRQSVHRKLALALKPGACLILEAFHPAQLQYASGGPKDLDMLYTQ
                     ELLGADFGGLLQSVMAWDGEVTLSEGPGHQGLAHVTRWVGVRSE"
     gene            2067..2447
                     /locus_tag="PKX82_09735"
     CDS             2067..2447
                     /locus_tag="PKX82_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOF52576.1"
                     /translation="MKTAHDLVAAAKSRVQEISIADAEQAIRDADVLVDVREAEEFAA
                     GHIGGAVHMSRGLLEFKFSGNPALQARDTKVVLYCKTSGRAALAACALHDMGYLNVKS
                     IAGGFDAWAAAGKPVAKPEAPSFD"
     gene            complement(2451..2852)
                     /locus_tag="PKX82_09740"
     CDS             complement(2451..2852)
                     /locus_tag="PKX82_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52577.1"
                     /translation="MELSKTLKVLGVIAIVFGALTIFSGGRALFGGDEARAAVGHAVG
                     FVLWFNFLAGFAYVLTGVGILARKPWATATALALALSTGGVALAFGLHVINGGAYEIR
                     TVGALALRLGFWMVAAKIAGPLWAHRRTLQP"
     gene            complement(2891..>3303)
                     /locus_tag="PKX82_09745"
     CDS             complement(2891..>3303)
                     /locus_tag="PKX82_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52578.1"
                     /translation="LIPFLDVTDNVALLPMLAGVPNAQARLRALELLTALDVQHRAKA
                     QPSQLSGGEQQRVSIARALANRPPVILADEPTAPLDSERALGVMRILNQMAQHAQTAI
                     IVVTHDEKIIPTFKRIYHIRDGHTFEEAGEGRDL"
BASE COUNT          593 a          990 c         1061 g          659 t
ORIGIN      
        1 atgtgatcgt gacccactac catgcggacc acatctacgg tctgcaggcc ttcaaggcga
       61 agggcgcgcg catcatcgcg caccaggcgg cccgtgagta cctgaactcc gatacggcgc
      121 ggctgcgact gattgcgtcc cgacaggagc tcgcgccgtg gattgatgag tccacccgtc
      181 tggtgcctgc cgatgaatgg atcactggcc ccagcgagct ggtcgtgggc ggagtgcgtt
      241 tggtgctgca acccgtgggg ccgtcgcata cgccagagga catcgtggtg tacctgccgg
      301 agcagaaagt tctgtttgcc ggcgatctgg tgtttcgcag ccgcattcca tttgtgggcc
      361 aggccgacag cggccactgg atcaaggcgc tggataccct gctggcattc gatgcgaaag
      421 tgattgttcc ggggcatggc ccgctgtcgt ccgaagcacg caaggacatg gaactgaccc
      481 gcgactatct ggcctacctg cgcagttcca tggggcaggc agccaggaac atggagcctt
      541 ttgaggatgc ctacaaggcc gcggactgga tgaagttcga acacttgccg ctgtttcgtg
      601 tcgccaaccg catgaacgct tacaacacct acctcctgat ggagaaagag gccccatgaa
      661 tgtgaacctg gacggtgttc gccgtcgctc cctgctggcc atcggtggcg gtgtcgctgc
      721 ctgcctggcg gtaccttcgg cctggtcggc cagcagcgca ttgccctatc cccaatcgct
      781 ggcggatgag ttggcgcgtg ccctcaaggt gggccaaccg ctggtggtga tggtcagtct
      841 cgacggctgt ccgttttgcc gcgtggcacg cgacagttac ctggccccca tgatgcggca
      901 ggagggcgtg cccgtggtgc agatcgatat gcgcacagca gttgccgtgc gggatttcag
      961 gcagtttgcg atgacgcacg atgcactggc ccgtgcgtgg ggaatcaagg tcgcgccgac
     1021 ggttctgttc ttcggaccgg gtggtcaaga ggtcgcggag cgcctggtgg ggggctacat
     1081 cccggatttt tatggtgcct atctggacca gcgcatgcag acggctcgcg cagccatcaa
     1141 gccatgatcc ggcgcatccc tgtgctgtgg ctggcggtcg ttgcaggttg gttggcaatc
     1201 gcacccctcg tgccggaacc ccatctggtg gaaaaaacac gcatgctttt gtcgggcact
     1261 ttggtgcgcc ccatcgatat ctttgacctg ttgctgcact cggtgccact catgctgctg
     1321 gcatggcgtc tggtcgtgat ggcacaggat agccgcgaca aaccctgaga atcgtgcgat
     1381 gcattcaccc ctgaacttct gggaccagcg ttttgcggag cctggcttca agtacggcac
     1441 cgagcccaat gcattcctgc gtgaacaggc cgtgcgcctg ccacccgcgg gtcgtgtgtt
     1501 ggtgccggga gatggggaag ggcgcaatgg tgtctggctg gctgcacagg ggcaccaggt
     1561 ggcatctgtc gacagttcgg ccgtgggctt gcagaaggcc cgcgatctgg ccagccagca
     1621 tggcgtggaa ctggagaccg tggtggcaga cctggccgtc tgggagccgg cgccagccag
     1681 tgtagatggc gtggtgctga tctataccca tctgcccggt tcgatccgcc agagtgtgca
     1741 ccgcaagctt gcgctggcgc tcaagcccgg cgcctgcctg atcctcgaag ccttccatcc
     1801 ggcccagctg caatacgcca gtggcggccc caaagatttg gacatgctgt atacccagga
     1861 actgctcggc gcagattttg gtggtttgct ccagtccgtc atggcctggg acggtgaagt
     1921 gacgctttcc gaaggtcccg gtcaccaggg gctggcccat gtgacccgct gggttggcgt
     1981 gcgttcagag tgaaattggc ctctagcccc cgtatctatt gcgcgagtag ctattatttt
     2041 tgtagtaatt gaatttggag aaatgtatga agactgccca tgacctcgtt gccgccgcta
     2101 aatcccgcgt gcaggaaata tccatcgccg atgccgagca ggcgatccgc gatgccgatg
     2161 tgctggtgga cgtgcgcgag gccgaagagt ttgccgcagg ccacattggt ggagccgtcc
     2221 acatgtcgcg cggcctgctg gagttcaagt tcagtgggaa cccggcactg caggcgcgtg
     2281 ataccaaggt ggtgctgtat tgcaaaacca gtggccgggc cgcactggcg gcgtgcgccc
     2341 tgcacgacat gggctacctg aatgtgaaat ccatcgccgg tggcttcgac gcctgggctg
     2401 ccgcgggcaa gcccgtcgcc aagcccgaag ccccctcgtt cgattgaggc ctagggttga
     2461 agggtacgcc ggtgagccca caggggcccg gcaatttttg ctgccaccat ccagaacccc
     2521 aaacgcagcg ccagcgcacc gacggtacgg atttcatagg cgcccccgtt gatcacgtgc
     2581 agcccaaaag caagagcgac gccacctgtg gatagcgcca gcgccagagc cgtggcggtt
     2641 gcccagggct ttcgcgccag aatcccgacg cccgtcagca cgtaggcaaa tcccgccaga
     2701 aagttgaacc acagtacaaa gccgaccgca tggcctacgg cggcacgtgc ttcgtccccg
     2761 ccaaacagcg cccgaccacc gctgaagatg gttaatgcac caaagacaat cgcgatgaca
     2821 ccgagtacct tgagcgtttt cgatagttcc atagccagtc ctttcaagtg gggtagcccg
     2881 gcttgcgccg ttacaggtcg cgtccttctc ccgcctcctc aaaggtgtgc ccatcacgga
     2941 tgtggtaaat ccgcttgaag gtcgggatga tcttttcgtc gtgggtgacc acgatgatgg
     3001 cggtctgcgc atgctgtgcc atctggttca ggatgcgcat gacgcccaaa gcccgttcgc
     3061 tgtccagtgg ggctgtgggc tcgtccgcca gaatcaccgg tgggcggttg gccagagccc
     3121 gtgcgatgga aacgcgctgt tgctcgcctc ctgacaactg cgatggctgt gccttggcgc
     3181 ggtgctgtac atccagcgca gtcagcagct ccagtgcacg caaccgcgcc tgggcattgg
     3241 ggacgccagc cagcatgggc agcagcgcga cgttgtcggt gacatccaga aacgggatca
     3301 ggt
//
LOCUS       DAOSJM010000277         3296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25315_length_3296_cov_4.136378, whole genome shotgun sequence.
ACCESSION   DAOSJM010000277 DAOSJM010000000
VERSION     DAOSJM010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25315_length_3296_cov_4.136378"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..406
                     /locus_tag="PKX82_09750"
     CDS             209..406
                     /locus_tag="PKX82_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOF52579.1"
                     /translation="MNQNFTVTGMTCGHCEKAVTRALHQVDPQAEVKIDRTQNLVQVE
                     STQPREALAAAITEEGYAVAA"
     gene            390..845
                     /gene="cueR"
                     /locus_tag="PKX82_09755"
     CDS             390..845
                     /gene="cueR"
                     /locus_tag="PKX82_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003072094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HOF52580.1"
                     /translation="MPLPHEPLKHDSTSDFPVSIGEAARRSGVSAKMLRHYESLGLLG
                     RVHRTDSGYRQYSEADVHTLRFIKRCRDLGFSMAEIAELVNLWQNRRRASASVKRIAQ
                     KHMDELAARIEALQTMQRTLQGLLHHCHGDERPDCPILDDLSGATTLPA"
     gene            842..1492
                     /locus_tag="PKX82_09760"
     CDS             842..1492
                     /locus_tag="PKX82_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF52581.1"
                     /translation="MTDPVSLYPALAQVAPTLESLGQTVGPMTVPAGTVLFDENSPCR
                     GFPLVLEGEVKVSRHSGDGRSLELYRVVPGELCLVSSACLFRSTPLSAQGITTRPTTL
                     LLIPPPIFNQWIATPAFRDNVLGLFAERMADLTGLIDAVAFHKLDQRLAAALLGRGQD
                     LALTHQTLADELGTVREIITRLLRRFEREGWVELSRERIHISNSAALRALASALTL"
     gene            1563..1754
                     /locus_tag="PKX82_09765"
     CDS             1563..1754
                     /locus_tag="PKX82_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOF52582.1"
                     /translation="MTHNVGNIERIIRIVAGLVLIALAATGTVGWWGWLGIVPLATGA
                     LGWCPPYAIFGINTCSMKK"
     gene            1970..2593
                     /locus_tag="PKX82_09770"
     CDS             1970..2593
                     /locus_tag="PKX82_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52583.1"
                     /translation="MLKLSTRSSASRKGLSALAASGIVVLAALTAPAHANDIGTLLGG
                     GVGAAAGAVLGQSVGGRQGAVIGGAIGGATGAAVSTHGRGHNGAVIGAAVGGAAGAAV
                     GQSAGGRNGAVLGAGAGGAAGAAIGRGVVSQPHHPQPVYQASGPVVHHYPVAYGPVYQ
                     PVYQRPPVHGYGPGWDRHDGRRDRWERQQRHHSHGRGHGWGHDGRGH"
     gene            complement(2604..>3296)
                     /locus_tag="PKX82_09775"
     CDS             complement(2604..>3296)
                     /locus_tag="PKX82_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOF52584.1"
                     /translation="VLPGTVVKRKAEEQIQHIEATTGRKPGKKESREIREDARLALLP
                     LAFTKQSTVLVWIDPQAKTLVLSAGSQARADEVMTCLIKAMDGLSVQLINTQMSPAAA
                     MSVWLSTKEAPQSFSVDRECELKAADESKAVVRYTRHPLDTDEVSQHIANGKVPTRLA
                     MTWNDHVSFVLTEALQLKKVAVLDVVFEGAGAAGDGKEDEFDTDVAIATGELRKLIPD
                     LLDALGGEVVPA"
BASE COUNT          576 a         1081 c         1012 g          627 t
ORIGIN      
        1 tgcccgctcc acccgggata cacaggaggc acaggtcatg ccaccaatgc cgatgtccag
       61 cgaggaggag gaaggagtat ttgtattcat ggtgtgtagc gtaatccttc ccatgatggc
      121 aaggtcaagc caaggggcac ttgaccttcc cacgatagaa aggttcagac tatgatttcc
      181 tgtccaacca agtcaaagga tccccatcat gaaccagaat ttcaccgtca ccggcatgac
      241 ctgcggccac tgcgagaaag ccgtcacccg cgcgctgcat caagtggatc cccaggccga
      301 ggtgaagatc gaccgcaccc agaacctggt gcaagtggaa tccacccagc cgcgcgaggc
      361 gctggccgcc gccatcaccg aagagggcta tgccgttgcc gcatgagccg ctgaagcacg
      421 acagcaccag cgactttcct gtttccatcg gggaagccgc acggcgctcg ggtgtttcgg
      481 caaagatgct gcgccactac gagtcgctgg gcctgctggg ccgcgtccac cgcaccgaca
      541 gtggctaccg ccagtacagc gaggccgatg tacatacgct gcgcttcatc aagcgctgcc
      601 gggatctggg cttttccatg gccgaaattg ccgagctggt caacctgtgg caaaaccgcc
      661 gccgcgccag cgccagcgtc aagcgcatcg cacaaaagca tatggacgaa ctggcagccc
      721 gtattgaggc tttgcagacc atgcagcgca ccttgcaggg cctgctgcac cactgccacg
      781 gcgacgagcg cccggactgc cccattctgg atgacctttc cggcgccact acactgcccg
      841 catgaccgat cccgtttcgc tgtaccctgc gctggcacaa gtcgctccaa ccctggaaag
      901 cctggggcag accgttggcc ccatgacggt accggcgggg acggtgctct tcgacgagaa
      961 ctccccgtgc cgcggctttc ccctggtgct cgaaggggag gtcaaagtct cgcgccattc
     1021 cggcgatgga cgctcgctgg agttgtaccg cgtcgtgccc ggcgagttgt gcctggtctc
     1081 cagtgcctgc ctgttccgca gcaccccgct gtcggcgcag gggattacca cccggcccac
     1141 caccttgctg ctgattccgc cacccatctt caaccagtgg attgccaccc ctgcctttcg
     1201 cgacaacgtg ctgggcctgt ttgccgaacg catggccgac ctcaccggcc tgatcgatgc
     1261 cgtggcgttt cacaagctgg accagcggct ggcagcggcc ctgctcggcc gggggcagga
     1321 cctggcactg acccaccaga cactggccga tgagctgggc acggtgcgcg agatcatcac
     1381 ccgcctgctg cgccgctttg agcgcgaagg ctgggtggag ctgtcacgcg agcgcatcca
     1441 catcagcaac agtgcagcgc tgcgcgcgct ggcctccgcg ttgactctgt gacccaagtc
     1501 acatacgctg ggtccgcgcg gggttccaat acgcccactg attcattttt aaggagtttt
     1561 ccatgacaca caacgtcggc aatatcgaac gcatcatccg catcgttgcc ggtctggtgc
     1621 tgatcgcact ggccgctaca ggcactgttg gctggtgggg ttggctgggc atcgttccgc
     1681 tggccaccgg tgcgctgggc tggtgccctc cctatgccat ttttggcatc aatacctgtt
     1741 cgatgaaaaa gtaactgccc ggcactcgct attgttttaa tagcttgttg cgcatatatg
     1801 acggggccta gggccccgtt ttgcattgaa tcatcgcagt caattccggc aggatccttc
     1861 tttacagatg taaaagcctg ttaacccggt catcgccgca cggcaggccg cgttagttga
     1921 tgacaaaccg ccacgtctgg cggtacgggc ccaggcccac ccaccatcca tgttgaaact
     1981 ttccacgcgc tcctctgctt cccgcaaggg tctctccgcg ctggcggcca gcggcattgt
     2041 cgttctggct gcattgactg cccccgccca tgccaacgac atcggaaccc tgctcggcgg
     2101 tggcgtcggt gcagctgcag gtgcggtact gggacagtcg gtgggcggac gccagggtgc
     2161 cgtgattggc ggcgccattg gcggcgcgac gggtgccgcg gtcagcaccc atggccgggg
     2221 gcacaacggt gccgtgatcg gcgcagccgt gggtggcgcc gcaggggccg ccgttggcca
     2281 gtcggccggc ggacgcaacg gtgccgttct gggcgctggc gcagggggtg ccgcaggggc
     2341 agcaataggc cggggcgtcg tcagccagcc acatcacccc caaccggtct accaggcttc
     2401 aggcccggtg gtgcaccatt acccggttgc atacgggccg gtctaccagc ctgtctacca
     2461 acgcccacca gtgcacggtt atggtccagg atgggaccgc catgacggtc gccgtgaccg
     2521 ctgggaacgc cagcaacgcc accacagcca tggtcgcgga catggctggg gccacgacgg
     2581 gcgcggccac tgatccctga gggtcaagca gggacgactt cgccacccag ggcgtcaagc
     2641 agatcgggga tgagtttgcg caactcgccc gtggcaatgg ccacgtcggt gtcaaattca
     2701 tcttccttgc cgtcgcccgc tgcaccggct ccttcgaaca ccacgtccag cactgccact
     2761 ttcttgagtt gcagcgcttc ggtcagcacg aaggacacat ggtcgttcca ggtcatggcc
     2821 aggcgggtgg gtacttttcc attggcaatg tgctggctca cttcgtccgt atccagcggg
     2881 tggcgggtat agcgcaccac cgccttggac tcatcggcgg ccttgagctc gcactcccgg
     2941 tccacgctga aactctgcgg cgcctctttg gtggacagcc agaccgacat ggcggcggca
     3001 ggtgacatct gggtattgat cagctgcacc gacaggccgt ccatggcctt gatcaggcag
     3061 gtcatcacct cgtcggcgcg ggcctggctg ccggcactga ggaccagggt tttcgcttgc
     3121 ggatcgatcc agaccagcac cgtggactgt ttggtaaacg ccagcggcag cagggccagg
     3181 cgggcgtctt cccggatttc gcggctttcc ttcttgcccg gcttgcgccc ggtggtggct
     3241 tcgatgtgct ggatctgttc ttcagccttg cgcttgacca cggtgccagg aagcac
//
LOCUS       DAOSJM010000278         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25326_length_3295_cov_4.362654, whole genome shotgun sequence.
ACCESSION   DAOSJM010000278 DAOSJM010000000
VERSION     DAOSJM010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25326_length_3295_cov_4.362654"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1069)
                     /locus_tag="PKX82_09780"
     CDS             complement(143..1069)
                     /locus_tag="PKX82_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52585.1"
                     /translation="MLAFLRTGAVTTAALVFALPAWAVDVVFKPVTDGVFAYIGDTEG
                     RTYDNEGLNANIGLVVTPAGAVLIDSGATFQTARKIHEAARKVTTQPIQWVINTGGQD
                     HRWLGNGYFKSQGIETIAHAAAEADMKARASDHMEGLKVLKERLEGTVPTLPSRFVTG
                     QDTRLELGGVVMELKHRHGGHTPGDTLVWLPQKNVMFTGDVVYVDRTLGLIPVSRSKT
                     WLESFAVIDEIKPRTIVPGHGNVTNLATAQAHTRDLLLALRAHMKKAIDDGVDLDAAV
                     KSFNGKPFAVLKHADVWIPQLANQTYLEMERE"
     gene            complement(1257..1679)
                     /gene="arsC"
                     /locus_tag="PKX82_09785"
     CDS             complement(1257..1679)
                     /gene="arsC"
                     /locus_tag="PKX82_09785"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HOF52586.1"
                     /translation="MADITIYHNPACGTSRNTLALIRNSGAEPEVIEYLKTPPTKARL
                     QELLAAMGIHVRALLREKGTPYAELDLGNPKWTDDELLDCMLAHPILINRPIVVTPLG
                     VRLCRPSEQVLDILPQPQQGAFTKEDGEVVINAQGQRT"
     gene            complement(1701..2756)
                     /gene="arsB"
                     /locus_tag="PKX82_09790"
     CDS             complement(1701..2756)
                     /gene="arsB"
                     /locus_tag="PKX82_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOF52587.1"
                     /translation="MSEITKKLSFLDRHLTIWIFGAMAFGVGLGYFVPGVEQLINRFQ
                     SGTTNIPIAIGLILMMYPPFAKVRYEELPNVFRDFKVLGMSFILSWVVGPLLMFALAV
                     IFMPDKPDYMVGLILIGIAPCVAMVIVWNDLAKGSTEYAAGLVAFNSIFQVLFYSVYA
                     WFFISVLPPYFGLSAAIIDISIGQIAVTVLTYLGIPCIAGFLTRYAMLKFVSKDWYHG
                     QFVPKIGPITLVALLFTIVVMFSLKGKLIVTIPMDVVRIAIPLLIYFVAMFLLTFAVS
                     KYLGVGYQKSATCSFTAASNNFELAIAVAIAVYGIDSGAAFTAVIGPLVEVPIMIGLV
                     NVSLWLKSRYFTTAEQA"
     gene            complement(2768..3097)
                     /locus_tag="PKX82_09795"
     CDS             complement(2768..3097)
                     /locus_tag="PKX82_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOF52588.1"
                     /translation="MTDLDVVRALSALAQQSRLQIFRQLVVAGNTGMTPTQISAALGL
                     PGTGLSFHLKELANAGLITQERDGRNLIYRAAFGPMNEVLRFLTVNCCEGQSCELTEV
                     AASCENC"
     gene            complement(3094..>3295)
                     /locus_tag="PKX82_09800"
     CDS             complement(3094..>3295)
                     /locus_tag="PKX82_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF52589.1"
                     /translation="EKAAEDVGFYYAANAVGRFLGTLLSGLLYQWGGLMYALVGSALM
                     LIACWLVTLALPVTKPQTRIST"
BASE COUNT          715 a          909 c          991 g          680 t
ORIGIN      
        1 agaacagatg gcgggtgggg gtgttcagca aatccatggg ttttcgattc ctggtggtgg
       61 gtttatccag accccaaatt atgagtgaaa agtggcattg gcccccgtga aatatgcaca
      121 atatgctata aaaataggag cgctattcgc gttccatctc caaataggtc tggttagcaa
      181 gttgcggaat ccacacgtcc gcgtgcttga gcacggcgaa gggcttgcca ttgaagctct
      241 ttaccgcagc gtccaggtcc accccgtcat caatagcctt cttcatgtgg gcgcgcaggg
      301 cgagcagcag atcgcgcgtg tgggcttgcg ccgtggccag gttggtcacg tttccatgtc
      361 cagggacgat ggtgcgtggt ttgatctcgt cgatgactgc aaacgactcc agccaggtct
      421 tgcttcggct caccgggatc agccccagag tgcggtccac atagaccaca tcgcctgtga
      481 acatgacgtt tttctgtggc aaccacacca gcgtgtcgcc cggggtatgc ccgccatggc
      541 ggtgtttgag ttccatgacc acgccgccca gttcaagccg ggtgtcctgg ccggtgacaa
      601 agcgcgatgg aagcgtaggc acagtgcctt ccaggcgctc cttgagcacc ttcaaaccct
      661 ccatgtgatc gctcgcgcgg gctttcatgt cggcttcggc agcggcgtgg gcaatggttt
      721 cgatgccctg gcttttgaaa tagccattgc caagccaccg atggtcctgc ccgccggtgt
      781 tgatgaccca ttggatgggc tgggtggtca cctttctggc cgcctcatgg attttgcgtg
      841 ccgtctgaaa ggtggcaccg ctgtcaatca gcaccgcgcc tgcgggcgtc accaccagcc
      901 cgatgttggc gttcaagcct tcgttgtcgt aggtgcgccc ttcggtgtcg ccaatgtagg
      961 cgaacacacc gtcggtgacc ggcttgaaaa cgacatccac ggcccaggcg ggcagggcga
     1021 ataccagggc ggcagtggtc actgcgcctg tgcgaaggaa tgcgagcatg tggtgttctc
     1081 cagaatagtg gttttccgat tgtgaagacg aaccgaagcg tcaaaagacg cacgctttgc
     1141 cgaaatttat atgaaattgg cctctagccc ccgttccgta tgcgcaagca gctataaaaa
     1201 taatagcatt tcagagtggg atgcgtggca caaagtcctg cacatgccct gcatcgtcaa
     1261 gtacgctggc cttgtgcatt gatgaccact tcgccgtctt ccttggtgaa cgcgccttgc
     1321 tgtggctgcg gcaggatgtc cagcacctgc tcggaggggc ggcacaggcg cacgccgagc
     1381 ggagtgacga cgatggggcg attgatcagg atcgggtggg ccagcatgca atccagcagc
     1441 tcgtcgtccg tccacttcgg gttgcccagg tccagctcgg cgtagggcgt gcctttttcg
     1501 cgcagcaggg cgcgcacgtg gatgcccatg gccgccagga gttcctgcag gcgggccttg
     1561 gtaggaggtg tcttcaggta ctcgatgact tcaggttctg cgccactgtt gcggatcagg
     1621 gccagggtgt tacgggaggt gccgcaggcc gggttgtggt aaatggtgat atcagccatg
     1681 gaacatgtct ggatgccgct tcaagcctgt tccgcggttg tgaaataccg gctcttgagc
     1741 cacaacgata cgttgaccag tccaatcatg atgggcacct ctaccagtgg gccaatgacg
     1801 gcggtaaatg ccgctccgga gtcgatgccg tagacggcaa tggccacggc aatcgccaac
     1861 tcgaaattgt tgctggctgc cgtaaaggag caggtggcgc ttttctggta gcccacgccc
     1921 aggtacttgg acaccgcgaa agtgagcagg aacatggcca cgaagtagat cagcaggggg
     1981 atggcgatcc ggaccacatc catcgggatt gtgacgatca gttttccctt caggctgaac
     2041 atcaccacga tggtgaacag caaggcgacc aaggtgatgg gcccgatttt gggaacaaac
     2101 tggccgtggt accaatcctt ggagacgaac ttgagcatgg catagcgtgt caggaagcct
     2161 gcgatacagg ggatgcccag gtaggtcagc acggtcaccg cgatttgccc gatggagatg
     2221 tcgatgatgg ccgcggacag tccgaagtag ggtggtagta ccgagatgaa aaaccaggca
     2281 tacacgctgt agaaaagcac ctggaaaatc gaattgaaag ccaccagtcc tgcggcgtat
     2341 tccgtcgaac ctttggccag atcattccag acgattacca tggcaacgca gggtgcaatg
     2401 ccgatgagga tgaggcccac catgtagtca ggtttgtcgg gcataaagat cactgccagt
     2461 gcaaacatga gcaagggccc gaccacccaa ctcaggataa acgacattcc cagtaccttg
     2521 aaatcccgga agacattcgg cagttcttcg taccgcactt tggcaaaggg cggatacatc
     2581 atcaggatca gaccgatggc aatcgggata ttggttgtcc cggactggaa gcggttgatc
     2641 agttgctcaa ccccgggcac aaaatagccc agccccacac cgaaagccat ggcaccgaaa
     2701 atccagatcg tcagatgccg gtctagaaaa gaaagttttt tggtaatttc gctcacagcg
     2761 ccctcgtcta gcaattttca caagatgccg ccacctcggt cagttcgcac gactggcctt
     2821 cgcagcagtt cacggtgaga aagcgaagca cctcgttcat ggggccaaag gcggcgcggt
     2881 aaatcaggtt gcgtccatcg cgctcctggg taatcagccc tgcattcgcc agctctttca
     2941 aatggaaaga caggccggtg ccgggcaagc ccagtgccgc agaaatttgt gtgggtgtca
     3001 tgcccgtatt tccagcgacc accagttgcc tgaagatctg caaacgggat tgctgcgcca
     3061 gggccgagag ggcgcgtaca acatcgaggt ctgtcatgtg gaaatccttg tctgtggttt
     3121 ggtgacggga agcgccaggg tcaccagcca gcaggcgatc agcatgaggg ctgagccgac
     3181 cagcgcgtac atcaacccgc cccattggta gagcaggccg gacagcagtg tgcccaggaa
     3241 acgccccacg gcattggcag cgtagtagaa cccgacgtct tccgccgcct tctcc
//
LOCUS       DAOSJM010000279         3293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25354_length_3293_cov_4.934527, whole genome shotgun sequence.
ACCESSION   DAOSJM010000279 DAOSJM010000000
VERSION     DAOSJM010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25354_length_3293_cov_4.934527"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /gene="prs"
                     /locus_tag="PKX82_09805"
     CDS             <1..437
                     /gene="prs"
                     /locus_tag="PKX82_09805"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOF52590.1"
                     /translation="ARALAKQLNCDLAIIDKRRPKANVSEVMHVIGEIDGRNCVIMDD
                     MIDTAGTLVKAAEVLKARGAKKVYAYCTHPIFSGPAIERISQGDALDEVVVTNTIPLS
                     AAAAQCQKIRQLSVAPLIAETIMRIAKGESVMSLFSDQENLF"
     gene            562..1224
                     /locus_tag="PKX82_09810"
     CDS             562..1224
                     /locus_tag="PKX82_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOF52591.1"
                     /translation="MKFVAFERAKQGTGASRRLRVTGRTPGIVYGGNTDTTLIELDHN
                     ALWHALKKEAFHASVLDMELGGKEHKVLLRDVQMHPFKQLVLHIDFQRVDENTKLHMK
                     VPLHFSGDELSPAVKTDGCIANHVVTEIDVLCLPKDLPEFVAVDLSGLKKGKSLHLAD
                     IVLPKGVTAVTHGKNNPVIVSVVAVAGAVEAAPAADAAAAAAPAKGGKAAPAAKAPAA
                     KK"
     gene            1312..1953
                     /gene="pth"
                     /locus_tag="PKX82_09815"
     CDS             1312..1953
                     /gene="pth"
                     /locus_tag="PKX82_09815"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOF52592.1"
                     /translation="MIKLFVGLGNPGPEYEATRHNVGFWWIDALARQLKVSMAMDRSY
                     YGLVARTVINGQTVWLLEPQTFMNLSGKSVAALARFFKIAPQEMLVVHDELDIAPGEA
                     KLKLGGSHAGHNGLRDIHAQMGTDDYWRLRIGVGHPGVKSEVVNWVLKKPMLDHRIAI
                     DQCIDRTLKALPHLMAGDMDKATMLIHTSKPPRPKPPKPEKPALPAEPERTEP"
     gene            1950..2360
                     /locus_tag="PKX82_09820"
     CDS             1950..2360
                     /locus_tag="PKX82_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52593.1"
                     /translation="MRNLFKFATFFIAICAISTGAIAQKTVYRCGTTYSQTPCDGAVP
                     VNADDARSKTDKLEADKATQRDMQRAKDMEKVRLKEEKEALAQGKAASKTATKDAAKP
                     KSEPDEGDKKKSRHKKKEPEFFTAKSAPDSKKKD"
     gene            complement(2374..2643)
                     /locus_tag="PKX82_09825"
     CDS             complement(2374..2643)
                     /locus_tag="PKX82_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HOF52594.1"
                     /translation="MALIITDECINCDVCEPECPNEAIFLGPEIYEIDPAKCTECVGH
                     FDEPQCVQVCPVACIPVNPQFVEDKETLWQKYRRLQVLAKPASAA"
     gene            2721..>3293
                     /locus_tag="PKX82_09830"
     CDS             2721..>3293
                     /locus_tag="PKX82_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOF52595.1"
                     /translation="MPTPHDPSLPLHAWGASALLAAYRNRSLSPVQVTQAVLDHIAQW
                     EPHLCASYLLRPEKALEQARSSEARWFAGEPSGALDGVPVTIKENIATRGDPTPLGTL
                     ATDTTPAAADAPPAARLREAGAVMVCKTTMPDYGMLSSGVSTFHPLTRNPWDLSKTPG
                     GSSAGGGAAAAAGYGPLHMGTDIGGSLRLPA"
BASE COUNT          724 a         1075 c          922 g          572 t
ORIGIN      
        1 gtgcccgtgc cctggccaag cagctcaatt gcgatctggc catcatcgac aaacgtcgcc
       61 caaaagccaa tgtgtccgaa gtgatgcacg tcatcggtga aatcgatggc cgcaactgcg
      121 tcatcatgga cgacatgatc gacaccgccg gcaccctggt gaaagccgcc gaagtgctga
      181 aggcccgtgg tgccaagaag gtctacgcct attgcacgca ccccattttc tcgggtccgg
      241 ccattgagcg catctcccag ggcgatgccc tcgatgaggt cgttgtgacc aacaccattc
      301 ccctgagcgc tgccgctgcg cagtgccaga aaattcgcca gctctccgtg gcaccgctga
      361 ttgccgaaac aatcatgcgc attgccaagg gcgagtcggt gatgagtttg ttctcggatc
      421 aggaaaacct cttttgaggt ttctgcagaa gcagttctga tctgggacga aaagcgggcg
      481 ccactgcata gtgcagcacc aaggcgcctt tattaaaccg gagtcacact ggtcgcggtg
      541 gacttcctat tggagtaagt tatgaaattt gtcgcttttg agcgcgccaa gcagggcacg
      601 ggtgcgagcc gccgtctccg cgtcaccggc cgtacgcccg gcatcgtgta cggtggcaac
      661 accgacacca ccctgatcga actcgatcac aacgccctgt ggcatgccct gaagaaagag
      721 gctttccacg cttccgtgct ggacatggaa ctgggcggca aggaacacaa ggttctgttg
      781 cgtgatgtgc aaatgcaccc cttcaaacaa ctggttctgc acatcgactt ccagcgcgtg
      841 gacgaaaaca ccaagctgca catgaaggtg ccattgcact tcagcggtga cgaactgtcc
      901 cccgcagtca agaccgatgg ttgcatcgcc aaccacgttg tgaccgaaat cgacgtgctg
      961 tgcctgccca aggacctgcc cgagttcgtg gcagtggacc tgagcggact gaagaaaggc
     1021 aagtccctgc acctggctga catcgttctg cccaagggcg tgaccgctgt gacccacggc
     1081 aagaacaacc ccgtgatcgt ctcggttgtg gcggttgctg gcgcggtaga agcggctcct
     1141 gctgccgatg ccgccgccgc tgcagcccct gccaagggtg gcaaggctgc tcccgctgcc
     1201 aaggctcccg ccgccaagaa ataatcggct cacgcctttt attgctacgg cccacttcgg
     1261 tgggccgttt ttcttggtgc tgcccatttt tgctctacca tttcgatcca catgatcaag
     1321 ctctttgttg gcctgggcaa tcccggccct gaatacgaag ccacccgcca caacgtcggg
     1381 ttctggtgga tcgacgcgct ggcacgccag ctcaaagtgt ccatggcgat ggaccggagt
     1441 tactacggac tggtggcgcg caccgtgatc aatggccaga ccgtctggtt gctggagcca
     1501 cagactttca tgaatctctc gggcaaatcg gtggccgccc ttgccaggtt tttcaagatt
     1561 gcaccgcagg aaatgctggt ggtgcatgac gagctggata tcgcccccgg cgaggccaag
     1621 ctcaaactgg gcggcagcca tgccggacac aatggcctgc gtgacatcca tgcgcaaatg
     1681 ggtaccgacg actactggcg cctgcgcatc ggcgtgggcc accccggcgt gaagtccgaa
     1741 gtggtgaact gggtgctcaa gaaacccatg ctggaccacc gcatcgccat tgaccagtgc
     1801 atcgaccgca cgctcaaagc gctgccgcac ctgatggccg gggacatgga caaggccacc
     1861 atgctgatcc ataccagcaa gcccccgcgc cccaagccgc ccaagcccga gaaacccgca
     1921 ctcccagccg aacccgagag gactgaacca tgagaaacct gttcaaattt gctacatttt
     1981 ttatagctat ctgcgcaata tccacggggg ctatagccca gaaaaccgta taccgctgcg
     2041 gcacaaccta cagccagaca ccgtgcgacg gcgccgtccc tgtgaatgcg gacgatgcac
     2101 gcagcaaaac agacaaactg gaggcagaca aagccaccca gcgcgacatg caacgcgcca
     2161 aagacatgga aaaggtgcgg ctgaaagagg aaaaagaggc tcttgcccaa ggcaaagctg
     2221 ccagcaaaac ggcaaccaaa gacgcagcca aacctaagtc cgaacccgac gaaggcgaca
     2281 agaagaagtc aaggcacaag aaaaaggaac ccgaattctt caccgccaag tccgcaccgg
     2341 acagcaagaa aaaagactag cgcaccaccc gcgtcaggca gcagacgcgg gtttggccag
     2401 cacctgcaga cgccggtatt tctgccacag cgtttccttg tcttcaacaa actgggggtt
     2461 caccggaatg caggccaccg ggcacacctg cacacactgg ggctcatcga aatggcccac
     2521 gcactcggtg catttggcag ggtcaatctc gtagatctcc ggccccagaa atatcgcctc
     2581 gttcgggcac tcgggctcgc acacatcaca gttgatgcat tcgtcggtaa tgatcagcgc
     2641 cacagtgttt tctccatgtc gcgattatcg ccgccagcgc cataatggtt tgcccccgcc
     2701 gacaaaccct acctgctgcc atgcctacac cccacgatcc ctctttgccc ctgcacgcgt
     2761 ggggcgccag cgccttgctg gccgcgtacc gcaaccgcag cctgtccccg gtccaagtga
     2821 cgcaagccgt cctggaccac atcgcacagt gggagccgca cctgtgtgcc agctacctcc
     2881 tgcgcccgga gaaggcactg gaacaggcgc gttcctccga ggcccgctgg ttcgctggcg
     2941 aacccagcgg cgcacttgat ggcgtgcccg tcaccatcaa ggaaaacatc gccacccggg
     3001 gcgaccccac accgctgggc acgctggcca ccgacaccac gccggcggca gccgatgcgc
     3061 cgccagcagc gcggctgcgc gaagcgggtg cagtgatggt gtgcaagacc accatgccgg
     3121 attacggcat gctgtcctca ggcgtctcca ccttccaccc gctgacccgc aacccgtggg
     3181 accttagcaa aaccccgggc ggctccagtg ccggtggcgg cgcagcagct gcagccgggt
     3241 acggcccact gcacatgggc accgacattg gcggctccct gcgcctgcca gcc
//
LOCUS       DAOSJM010000280         3283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25448_length_3283_cov_4.921004, whole genome shotgun sequence.
ACCESSION   DAOSJM010000280 DAOSJM010000000
VERSION     DAOSJM010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25448_length_3283_cov_4.921004"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="PKX82_09835"
     CDS             complement(<1..470)
                     /locus_tag="PKX82_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HOF52596.1"
                     /translation="MKAKTGKTAKAKPAAKAPAKPVAKKNVKPVAKPVTKASAKPVAK
                     KAVKAPVKVAPKAVAKPAAKPVAKAAPKAKATAKPVVKAVAKPVPKAAPKVVAKPVAK
                     TPVKAAVPVKPVVKSAAKPAVVASVPKEKVVTPMPQAMPKAAPAPVAPAQWQFP"
     gene            complement(709..1776)
                     /locus_tag="PKX82_09840"
     CDS             complement(709..1776)
                     /locus_tag="PKX82_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOF52597.1"
                     /translation="MSLIPATILTGFLGSGKTTLLKRVLAEAHGQKIAVIENEFGEEN
                     IDSDILVSDTNEQIIQMSNGCICCTIREDLRATLRDLAEKKRKGELNFERVVIETTGV
                     ADPGPVAQTFFMDDEIAETYLLDSILTLVDAKHAAQQLNDRQEARRQVGFADQIFISK
                     SDLVSKDELEDLMHRLKHMNPRAPQKAVHFGDVSLAEVFDLRGFNLNAKLDIDPDFLK
                     EDDHDHGHDHHDHAPGEACNHPSHAHEGEHGHGHHHHHDDDVKSFVFKSERAFDPAKL
                     EDFLGAIVNIYGPRMLRYKGVLFMKGTDRKVIFQGVHQLMGSDLGPAWGEGEQRISKM
                     VFIGIDLPKDILLQGLEQSLV"
     gene            complement(1829..>2599)
                     /locus_tag="PKX82_09845"
     CDS             complement(1829..>2599)
                     /locus_tag="PKX82_09845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF52598.1"
                     /translation="ALLAATGLTRLYERSDASSRTLEGLPEATGWLRGDGPTDLVLQE
                     HEWKLALSIAEGHKTGFYLDQRDSRKKFAEYTRRLGFQRVLNCYCYTGGFTVAALSGG
                     AVHVTSIDSSGPAIEKAAANVALNGFEASRADFMDADVNASLRKFAQEGRTFDAIVLD
                     PPKFAPTVAHAERAARAYKDINRLAFKLLEPGGVLFTYSCSGGISGDLFHKIVASAGC
                     DAGVDGFITERMGGAPDHPMTIYFPEGEYLKGLVVMRK"
     assembly_gap    2600..2609
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2610..3018)
                     /locus_tag="PKX82_09850"
     CDS             complement(<2610..3018)
                     /locus_tag="PKX82_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806779.1"
                     /note="SAM-dependent;catalyzes the methylation of cytosine
                     at position 1962 of the 23S rRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HOF52599.1"
                     /translation="MKTIRLRAGKERSLLRGHPWIFESAIAKGGGDSGETVRVDSAEG
                     QFLGWAAFSPSSKIRARVWSFDEKQRIDASFFIAACASSVRARARFDIKSDGMRLVHG
                     ESDGLPGLIVDRYGDTLVAQFTSAGVEKWKDVIA"
BASE COUNT          584 a          984 c          984 g          721 t
ORIGIN      
        1 ttggggaact gccactgggc cggcgccaca ggtgcgggcg cggctttggg catggcttgg
       61 ggcatggggg taactacttt ttctttgggt acggaggcaa ccacagccgg tttggctgca
      121 gatttgacaa cgggtttaac aggaacagca gccttgacgg gtgtcttggc taccggcttg
      181 gctaccactt tgggagcagc cttgggcaca ggtttcgcca cggccttgac tacgggtttt
      241 gctgtggctt tggccttggg agcggctttg gctaccggct tggcagcagg cttcgcgaca
      301 gccttgggtg ccacttttac tggtgccttt acggctttct tggccactgg ttttgccgat
      361 gctttggtta ccggcttggc aacaggcttc acatttttct tggccactgg tttggctggc
      421 gccttggctg ctggtttggc ttttgcggtc ttcccggtct tggctttcac ttcttgtctc
      481 ctggcagggc tggcctgcgc gtggtctttc gtgccttggt ccccgctgcg ggtctttgtg
      541 gaccgatctg gcgaggtttc tccggcgcgc gagtgtacag gcttccccat aggaaaaccc
      601 atcagttgca aaatcgagac aatcatttgg ctccctgcca gtaattagta agtctgggga
      661 cagttacaga cacgttcgtg tgacatccga acgggaatgc gtcgttcgtt acaccagcga
      721 ttgctccagc ccctgcaaaa gaatgtcctt gggcagatcg atgccgatga acaccatttt
      781 gctgatgcgt tgctcgcctt ccccccaggc aggccccaag tcgctgccca tcaactggtg
      841 cacgccctga aagatcacct tgcggtcggt gcccttcatg aacagtactc ccttgtagcg
      901 cagcatgcgg ggtccataga tattgacgat ggcccccaga aagtcttcca gtttggccgg
      961 atcgaatgcc cgctcggact tgaagacaaa gcttttgaca tcgtcatcgt gatggtgatg
     1021 atggccatgg ccgtgttccc cttcatgggc gtgcgacgga tggttgcagg cctccccggg
     1081 cgcatggtca tggtggtcat gcccatggtc atggtcgtct tccttcagga agtccgggtc
     1141 aatgtccagc ttggcgttca gattgaagcc gcgcaggtca aacacctcgg ccagcgacac
     1201 atcgccaaag tgcaccgcct tttgcggggc acgcggattc atgtgcttga gccggtgcat
     1261 caggtcttcg agctcgtctt tggacaccag gtccgacttg ctgatgaaga tctggtcggc
     1321 aaaacccacc tggcggcgcg cttcctggcg gtcgttgagc tgctgggcgg cgtgctttgc
     1381 gtccaccagc gtcagaatgg agtccagcag gtaggtctcg gcaatctcgt cgtccatgaa
     1441 gaaggtctgt gccaccgggc cggggtcggc cacaccggtg gtctcgatca ccacgcgctc
     1501 aaaattcagc tcacccttgc gtttcttctc ggccagatcg cgcagcgtgg cacgcaggtc
     1561 ttcgcggatg gtgcagcaga tgcagccgtt gctcatctgg atgatctgct cgttggtgtc
     1621 gctcaccagg atgtcgctgt cgatgttttc ctcgccaaac tcgttctcta tcacggcaat
     1681 cttctgcccg tgggcctcgg ccagcacgcg tttgaggagg gtggttttgc cggacccaag
     1741 aaatccggtc aggatggtgg cgggaatcag agacatgtgg gaacccaaaa aacagcagcc
     1801 cggagtgtag cgttctattg tggcggcgct atttgcgcat caccaccaac cctttgaggt
     1861 actcgccttc cggaaagtaa atggtcatgg gatggtctgg tgcgccgccc atgcgctcgg
     1921 tgatgaagcc atccactccc gcatcgcaac ccgcagacgc cacgatcttg tggaacaggt
     1981 cgccactgat gccgcccgag cagctgtagg taaacagcac gcccccaggc tccagcagct
     2041 tgaatgccag gcgattgata tccttgtagg cccgggccgc gcgctcagcg tgcgccacag
     2101 tgggtgcaaa cttcggagga tccagaacaa tggcgtcaaa ggtgcgcccc tcctgcgcaa
     2161 acttgcgcag cgaggcattg acgtccgcat ccatgaagtc ggcccggctg gcctcaaacc
     2221 cgttgagcgc cacgttggcg gcggctttct caatggctgg gccgctggaa tcgatggacg
     2281 tgacgtgcac cgcaccgccc gagagggccg cgacggtaaa gccaccggtg tagcaatagc
     2341 agttcagcac ccgctggaag cccagccggc gggtgtactc agcaaacttc ttgcggctgt
     2401 cgcgctggtc caggtaaaag ccggtcttgt ggccttcggc aatggagagt gccagtttcc
     2461 attcgtgctc ctgcagtacc aggtcggtgg gcccgtcgcc gcgcagccag cccgtcgcct
     2521 cgggcaagcc ctccagcgtg cggctgctgg cgtccgagcg ctcatacaag cgggtcaacc
     2581 cggtcgcggc cagcagggcn nnnnnnnnnc ggcaatcacg tccttccact tttccacgcc
     2641 agccgaagtg aactgggcca ccagtgtgtc gccatagcgg tccacaatca gccccggcaa
     2701 cccgtcggat tcgccatgca ccaggcgcat gccgtcgctt tttatgtcaa atcgggccct
     2761 agcccgcaca gaacttgcgc aagcagctat aaaaaagcta gcgtctatgc gctgcttctc
     2821 gtcaaaactc cagacccggg cacgaatttt ggaactggga ctgaacgccg cccagcccag
     2881 aaactgccct tcggctgaat cgacccgcac tgtctcgccg ctgtcaccgc cgcccttggc
     2941 gatggccgat tcaaaaatcc agggatggcc gcgaagcagc gagcgctctt tgcccgcacg
     3001 aagtcgaatg gttttcatgg gggctattgt cgcttgggga ggtggccgac ggctgggcgc
     3061 gggagacgct ggacggcatg gcactcggct ccgttcgtgt cccccgccct gcgggctcct
     3121 cctttacctc actgcgccaa gcgccatgcc gtcctgagtt cggggcgttg caggttgtca
     3181 aggggcattc acgcaggcat ccaccacagt gtcattggtc tacgcaaata gctacatgga
     3241 agagaccgct ttcgcagcaa agcaaaggtc acttcagact gat
//
LOCUS       DAOSJM010000281         3278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25506_length_3278_cov_3.359293, whole genome shotgun sequence.
ACCESSION   DAOSJM010000281 DAOSJM010000000
VERSION     DAOSJM010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25506_length_3278_cov_3.359293"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PKX82_09855"
     CDS             <1..473
                     /locus_tag="PKX82_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOF52600.1"
                     /translation="HDEENDRWVAVHHPFTAPKDGHEDYMVTAPEKCISKGYDMVLNG
                     WEMGGGSVRIHRADVQQKVFDALKITPEEAQQKFGFLLDALQYGAPPHGGLAFGLDRI
                     VTLMTGAESIRDVIAFPKTQRAQCLLTQAPSMVDEKQLRELHIRLRNVDLAKTA"
     gene            526..990
                     /gene="nudB"
                     /locus_tag="PKX82_09860"
     CDS             526..990
                     /gene="nudB"
                     /locus_tag="PKX82_09860"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HOF52601.1"
                     /translation="MDKAFKIPQSVLVVIYTPALDVLLIERADAPGFWQSVTGSKDRL
                     DEAWEETATREVMEETGIDAHALGHTLTDWALENVYSIYPQWRHRYAPDVVQNTERVF
                     GLCVPMGTSVTLSAREHTAYQWLPWQEAADRCFSASNAEAVLHVPVFSARVT"
     gene            1009..1740
                     /locus_tag="PKX82_09865"
     CDS             1009..1740
                     /locus_tag="PKX82_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOF52602.1"
                     /translation="MTILRVATYNIHKGVQGVGPRRRLEIHNLGHAVEQLDADIVCLQ
                     EVRKVHHREAEYFTRWPDMPQADFLAPEGYEAVYRTNAFTKHGEHGNAMLSRWPVVAH
                     QHEDMSDHRFEQRGLLHSEVTVHGQSIHVVVVHLGLIRSSRVRQLAQLQQFIAREVPA
                     GAPVVVAGDFNEWGTLVRRTLGSIGLVALEKDRMATFPSRMPLVQLDHIYARGLIPVG
                     LEVPHGRIWWRMSDHLPLIAEFRLP"
     gene            1737..2957
                     /gene="clsB"
                     /locus_tag="PKX82_09870"
     CDS             1737..2957
                     /gene="clsB"
                     /locus_tag="PKX82_09870"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HOF52603.1"
                     /translation="MKGQRMRAGHEVQLLEGGTEYFPALVAAIEQASLEVRMETYIFH
                     FAAAGEQVAQALVRAASRGVAVFLVMDGIGTPRVPPEWAQRFNQAGVQWHQFSPLGRL
                     GLLIPGRWRRMHRKLCVVDGNVAFCGGINILDDYIDPNYGQLESARLDYAVRVSGPLV
                     ADAQAALVQFWVRLQVTRQLERLQFRRARAALDMTPVAVPVRVPDGVATYGAVFEHAA
                     ASLVLRDNVLNRTRIEWAYRKAIASAHHEVLIANAYFLPGTQLRRALMDAARRGVRVR
                     LVLQGRYEYFLQFHAARQLYGELLAAGVEIHEYQAGFLHAKVAVVDGRWATVGSSNLD
                     PLSLQLAREANVVVENRAFAHALQTRLEQVMVRSGRKMDPQAHAERPLKQRILGGFAV
                     GLVRLMLFLTGRRY"
     gene            3061..>3278
                     /locus_tag="PKX82_09875"
     CDS             3061..>3278
                     /locus_tag="PKX82_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOF52604.1"
                     /translation="MNPSDVDEGTPVSVKIRERLTAARKRFHANDNISDFIEPGELER
                     LLDEVEGKMKHVLDSMVIDTENDHNSKDT"
BASE COUNT          625 a          950 c         1073 g          630 t
ORIGIN      
        1 agcacgacga agagaacgac cgctgggtcg ccgtgcacca ccccttcacc gcacccaagg
       61 acggccacga ggactacatg gtcaccgcgc ccgagaagtg catctccaag ggctacgaca
      121 tggtgttgaa cggctgggag atgggcggtg gctcggtgcg tattcaccgc gccgacgtgc
      181 agcaaaaagt gttcgatgcc ctcaagatca cgcccgaaga agcgcagcag aagttcggct
      241 tcctgctcga cgccctgcaa tacggtgcgc ccccgcacgg tggcctggcc ttcggcctgg
      301 accgtatcgt gacgctgatg accggcgctg agtccatccg cgacgtgatc gccttcccca
      361 agacccagcg cgcccagtgt ctgctgacac aggcgcccag catggtcgac gagaagcagc
      421 tgcgcgaatt gcacatccgc ctgcgcaacg tggatctggc caagaccgcc tgattgccgc
      481 ggcttgctgc acaatgaaag ggcgctctgg tagcgccctt ttttcatgga caaagcattc
      541 aagattcccc aatcggtgct ggtggtgatc tacaccccgg cgctggatgt gctgctgatc
      601 gagcgtgccg atgcgccggg attctggcaa tcagttaccg gatccaaaga ccggctggac
      661 gaggcctggg aggaaacggc cacgcgcgag gtgatggagg aaacaggtat cgatgcccat
      721 gcactgggcc ataccctgac cgactgggcg ctggaaaatg tgtactccat ctacccgcag
      781 tggcgccacc gctatgcccc cgatgtggtg cagaacaccg agcgggtgtt tggactgtgc
      841 gtgccaatgg gtacttcggt aaccctgagc gcgcgcgagc atacggcgta ccaatggttg
      901 ccctggcagg aggccgcaga ccggtgtttt tctgcatcga atgccgaggc agttctacac
      961 gtacctgtct tttcggccag agtcacctga cactggcatg atgtcaccat gactattttg
     1021 cgtgtcgcca cctacaacat ccacaagggc gtgcagggcg taggcccgcg gcgccggctg
     1081 gaaatccaca acctgggcca cgcggtggag caactggacg ccgacattgt gtgcctgcag
     1141 gaggtgcgca aggtccacca ccgggaggcg gagtacttca cccgctggcc agacatgccg
     1201 caggctgact ttctggctcc agagggttat gaggcggtgt accgcaccaa cgcatttacc
     1261 aaacacggtg aacatggcaa cgccatgctg tcgcgctggc cggtggtggc gcaccagcac
     1321 gaggacatgt cggaccaccg gtttgaacag cgtggcctgt tgcactccga agtgacagtg
     1381 cacgggcaat cgattcatgt ggtcgtggtg cacctcgggc tgatacgctc tagccgggtg
     1441 cgccagttgg cgcaattgca gcagttcatc gcacgggagg tgcctgcagg tgccccggtg
     1501 gtggtggcgg gtgatttcaa tgagtggggt acgctggtac gccgcacgct gggcagtatc
     1561 gggctggttg cactggagaa agaccgcatg gcgacctttc catcgcgcat gcccctggtg
     1621 cagctcgatc acatctacgc ccgagggttg atccctgtag ggttggaggt tccgcatggc
     1681 cgcatctggt ggcgtatgtc cgaccatctt ccgctgattg ccgaattcag actgccatga
     1741 agggacagcg catgcgtgcc ggccacgagg tgcagttgct cgaaggcggc accgagtatt
     1801 tcccggccct ggtggctgcg attgaacagg ccagtctgga agtgcggatg gagacctata
     1861 tcttccattt cgcagctgca ggtgagcagg tggcgcaggc gctggtacgc gcggccagcc
     1921 ggggcgtggc cgtatttctg gtcatggacg ggattggtac gccacgcgtg ccacccgagt
     1981 gggcgcagcg gttcaaccag gccggtgtgc aatggcacca gttctcgccg ttgggccggc
     2041 tgggattgct gattcccgga cgctggcggc gcatgcaccg caagctgtgt gtggtggacg
     2101 gcaatgtggc attttgcggc ggcatcaaca tccttgacga ctacatcgat cccaattacg
     2161 gacagctgga gtcggcccga cttgactatg ccgtgcgcgt gtcggggccg ctggtcgccg
     2221 atgcccaggc ggcgctggtg cagttctggg tgcgtttgca ggtcacacgc cagttggaga
     2281 ggctccagtt ccgcagggca cgggctgcgc tggacatgac gccggtggcc gtaccggtgc
     2341 gggttcctga cggtgtggcg acgtatgggg cggtattcga acatgcggcg gccagtctgg
     2401 tattgcgtga caacgtgctc aaccgcaccc gcatcgagtg ggcctaccgc aaggccattg
     2461 cctcggcgca ccatgaggta ctgattgcca atgcgtactt tcttccggga acgcagttgc
     2521 gccgtgcgtt gatggacgcc gcgcgcaggg gtgtgcgggt gcgcctggtg ctgcaggggc
     2581 ggtatgagta tttcctgcag ttccatgcgg cgcgccagct ttatggcgaa ctgctggctg
     2641 cgggggtgga aatccatgag taccaggcgg gttttctgca cgccaaggtg gccgtggtgg
     2701 acggccgttg ggcgacagtg ggatcctcca acctggaccc attgagcctg caactggccc
     2761 gcgaggcgaa tgtagtggtg gaaaaccggg ccttcgccca cgcactgcag acgcgtctgg
     2821 agcaggtgat ggtccgcagt ggccgcaaga tggacccaca ggcccatgca gagcgccccc
     2881 tgaaacagcg tattctggga gggtttgcgg tgggtctggt gcggttgatg ttgtttctga
     2941 cagggcgacg ctattaattt gatagcttcc tacgcaatgg tgacgggggc tacacgctaa
     3001 aaaccctact cagcaaaggc tggtaacatt cttcacttgt taatgaagcc ccagagcacc
     3061 atgaaccctt ctgacgtcga cgagggaacg cccgtttccg tgaaaatccg ggagcgcctg
     3121 actgcagcgc gcaaacgctt ccacgccaac gacaatattt ccgacttcat cgaacctggc
     3181 gagttggaga ggctgctcga cgaggttgag ggaaagatga agcatgtgct cgacagcatg
     3241 gtcatcgaca ccgaaaacga ccacaactcc aaagacac
//
LOCUS       DAOSJM010000282         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25621_length_3267_cov_3.475716, whole genome shotgun sequence.
ACCESSION   DAOSJM010000282 DAOSJM010000000
VERSION     DAOSJM010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25621_length_3267_cov_3.475716"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="PKX82_09880"
     CDS             <1..1206
                     /locus_tag="PKX82_09880"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HOF52605.1"
                     /translation="RGELTVTYLTDAHRACAQRISMDMRNAGCDSVHIDAVGNVVGYY
                     KKDSASRISDGGWEPKTHKILLTGSHYDTVRNGGKYDGRLGIYVPLAVVQTLAAQKRR
                     LPFNLEIVAFAEEEGQRYKATFLGSGALTGDFQSEWLDQLDADGISMRQAMQHAGLCI
                     DDISKLQRNPDDYQGFVEVHIEQGPVLNELDLPLGIVTSINASVRYTGEVIGMASHAG
                     TTPMDRRRDAAAAVAELQLYVEQRALADGDSVGTVGILQVPNGSINVVPGRCQFSLDL
                     RAPSDAQRDALARDVLAQLQTICERRGVHHTLTETMRASAAPSAPKWQQRWEDAVSAL
                     GVPLYRLPSGAGHDAMKLHTLLPQAMLFVRGQNSGISHNPLESTTSDDMQLAVEAFLH
                     LVNDLARPT"
     gene            1219..2454
                     /locus_tag="PKX82_09885"
     CDS             1219..2454
                     /locus_tag="PKX82_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HOF52606.1"
                     /translation="MTPYEQLDAWIDAHFDEQVGFLQELIRVPTDTPPGNNAPHAERA
                     AELLDAMGLKTEKHPVPAALVHAAGLQTITNLVVHRPYGKGPTIALNAHGDVVPPGEG
                     WTHDPYGGEIESGKIYGRAAAVSKCDFSTFSFAVRALESLGIPLHGGVDLLFTYDEEF
                     GGEVGPAWLLQNKLTKPDLMIAAGFSYQVVTAHNGCLQMEVTVHGKMAHAAIPDSGID
                     ALQGTVKILNALYAQNTLYKQVCSKVEGITHPYLNVGMIEGGTNTNVIPGRVSFKLDR
                     RMIPEENPVEVEATLRQVIAEAAASFPGIMVEVKRILLANALRPLPGNKPLVDALQKH
                     ASTVFGEPIPALGTPLYTDVRIFCEAGIPGVIYGAGPRTVLESHAKRADERLDLQDLR
                     RATKVIARALLDLLGTAKA"
     gene            complement(2451..>3267)
                     /locus_tag="PKX82_09890"
     CDS             complement(2451..>3267)
                     /locus_tag="PKX82_09890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF52607.1"
                     /translation="VAVDQWVTPNITPYLVGCVLCALVFYIRPVPSAWFYALSYLVFF
                     YALGETQDSAPQLLSNRLNGLAICIIGWVMSVMLWRKFTTISLQQNLLTRANTELQNK
                     QRDLERLTRQDGLTGLFNRNTFVELAKQELDRAQRQGSATSILLLDLDHFKLVNDTWG
                     HPAGDAVLRNVALVASATVRSTDLVGRLGGEEFVVLLPGTTTEAARKLAEKLRQRLQA
                     SPTPWESGSITATASIGLAGTTAAEKRPFDALYHDADKALYLAKLRGRNRVEP"
BASE COUNT          665 a         1066 c          984 g          552 t
ORIGIN      
        1 cgcggcgaac tcaccgtgac gtatctgacc gatgcccacc gcgcctgtgc gcagcgcatc
       61 agcatggaca tgcgcaacgc cggttgcgac agcgtgcaca tcgacgctgt cggcaatgtg
      121 gttggttact acaaaaaaga tagcgcatcg cgcatatccg acgggggctg ggagcctaaa
      181 acgcataaaa tcctgctcac cggcagccac tatgacacgg tgcgcaacgg cggcaagtac
      241 gacgggcgcc tgggcattta tgtgccgctg gccgtcgtgc aaaccctggc tgcgcaaaag
      301 cgccgcctgc ccttcaatct ggaaatcgtc gcctttgccg aagaagaagg ccagcgctac
      361 aaggccacct tcctgggctc gggcgcactg acaggtgact ttcagagcga gtggctggac
      421 cagctcgatg ccgatggcat ctccatgcgc caggccatgc agcacgcagg cctgtgcatc
      481 gacgacattt ccaagctcca gcgcaaccca gacgactacc agggttttgt cgaggtacac
      541 atcgagcagg gcccggtgct caacgagctt gacctgccgc tgggaatcgt gacttccatc
      601 aacgccagcg tacgttacac cggcgaagtc atcggcatgg ccagccacgc tggcaccacg
      661 cccatggacc gcagacgcga cgccgccgcg gcagtggcgg agttgcagct gtatgtggaa
      721 caacgcgcgc tggccgatgg cgattcggta gggaccgtgg gcatcctgca agtgcccaac
      781 ggttccatca acgtggttcc ggggcgctgc cagttttcgc tggacctgcg agcgcccagc
      841 gatgcgcaac gtgatgcact ggcccgcgat gtactggccc agttgcaaac catttgcgaa
      901 cgccgtggag tgcaccacac gctgaccgag accatgcgcg ccagcgcagc ccccagcgcg
      961 cccaagtggc agcaacgctg ggaagatgca gtctccgctc tgggtgtccc gctctaccgg
     1021 ctgcccagcg gcgccgggca tgatgcgatg aagctgcaca ccctcctgcc gcaggccatg
     1081 ctgtttgtac gcggccagaa ctccggcatc agccacaacc cgctggagtc gaccaccagc
     1141 gacgacatgc aactggcagt cgaggccttt cttcatctgg tcaacgatct cgctcgcccc
     1201 acctgaaacc acaccaccat gaccccctac gaacaactgg acgcctggat cgacgcacac
     1261 ttcgacgagc aggtgggctt cttgcaggaa ctcatccgcg tccccaccga cacgccaccg
     1321 ggcaacaacg caccgcatgc ggaacgcgca gccgagctgc tcgatgccat gggtctgaag
     1381 accgaaaaac acccggtacc cgcagcactg gtgcatgccg cgggcctgca aaccatcacc
     1441 aacttggtag tgcaccgtcc gtacggcaaa ggccccacga ttgcgctcaa cgcccatggc
     1501 gacgtagtac cgcccggcga aggctggacg cacgacccct acggtggcga gatcgaaagc
     1561 gggaagatct atggccgcgc agccgcagtt agcaagtgcg acttctccac cttttcgttt
     1621 gcggtgcgcg cgctcgaatc gctgggcata ccgctgcacg gcggtgtgga cctgctgttc
     1681 acctacgacg aagagtttgg tggcgaagtg ggcccggcct ggctgctgca gaacaagctc
     1741 acaaagccag acctgatgat cgccgccggt ttttcctacc aggtggtgac cgcgcacaac
     1801 ggctgcctgc agatggaagt caccgtgcac ggcaagatgg cgcatgccgc cattcccgac
     1861 tccggcatcg acgccctgca gggtacggtg aagattctca acgcgctgta tgcacagaac
     1921 accttgtaca aacaggtgtg ctccaaggtt gagggcatca cccaccccta tctgaacgtc
     1981 ggcatgatcg aaggtggtac caataccaat gtgattcccg gccgcgtatc cttcaagctt
     2041 gaccgccgca tgatcccgga agaaaacccg gtcgaagtgg aagccacttt gcgccaggtg
     2101 attgccgagg cggcagcgtc attcccgggc atcatggtgg aggtcaaacg cattttgctg
     2161 gccaacgcgc tgcggcccct tcctggcaac aagccgctgg tggacgcact gcaaaagcac
     2221 gccagcaccg tattcggcga acccattccc gcactgggca cacccttgta caccgatgtg
     2281 cgcatcttct gcgaagccgg cattcctggg gtgatctacg gcgccgggcc acgcaccgtg
     2341 ctggagtcgc atgccaagcg cgccgatgaa cgtctggacc tgcaggacct gcgccgtgcc
     2401 accaaggtca tcgcgcgcgc actgctggat ttgctgggga ccgctaaggc ttaaggttcc
     2461 acgcggttgc gtccgcgcag cttggcgagg tagagcgcct tgtcggcatc gtggtacagg
     2521 gcgtcaaacg gccgcttctc ggctgccgtg gtaccggcca ggccaatgct ggcagtggcc
     2581 gtgatcgagc ccgactccca cggcgtgggg ctggcctgca gacgctggcg cagcttttct
     2641 gccagcttgc gcgcggcctc agtggtggtt cccggaagca ggaccacaaa ctcttcaccg
     2701 cccagacgcc cgaccaggtc ggtgctgcgc accgtggcac tggccaccag tgccacattg
     2761 cgcaacacgg cgtcgccggc agggtggccc caggtgtcat tgaccagctt gaaatggtcc
     2821 aggtccagca gcaggatgga ggtagcactg ccctggcgct gggcccggtc cagctcctgc
     2881 ttggccagct cgacaaaggt attgcggttg aacaggccgg tcaggccatc ctgtcgggtc
     2941 aggcgctcca ggtcacgctg cttgttctgc aactcggtgt tggcacgggt caacaggttc
     3001 tgctgcaggg aaatggtcgt gaacttgcgc catagcatga ccgacatcac ccagcctatg
     3061 atgcaaatcg ccagcccatt gagccggttc gaaagcagtt gcggcgcact gtcctgcgtt
     3121 tcccccaagg catagaagaa caccagatag gacagggcgt aaaaccaggc ggacggaacg
     3181 ggacggatat agaacaccag ggcacacaac acgcagccaa ccaggtaggg cgtgatgttg
     3241 ggcgtcaccc actggtccac cgccaca
//
LOCUS       DAOSJM010000283         3265 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25645_length_3265_cov_3.865421, whole genome shotgun sequence.
ACCESSION   DAOSJM010000283 DAOSJM010000000
VERSION     DAOSJM010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25645_length_3265_cov_3.865421"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..618)
                     /locus_tag="PKX82_09895"
     CDS             complement(22..618)
                     /locus_tag="PKX82_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOF52608.1"
                     /translation="MSAPPATRAHPTSKTDLFVSFSLLALQGFGGVLAVVQRELVEKK
                     KWLTLEEFLEDWAVAQILPGPNVVNLSLMIGDRYFGMRGALAALAGMLTFPLVIALAV
                     AILFAGIADQPQVQGALRGMGAVAAGLITATGLKLIAALKENPMGAPICWAFAAITFV
                     AIGLLRIPLAWVLLVVGGAACAWTYRLLGQVAARSQQP"
     gene            complement(662..2074)
                     /locus_tag="PKX82_09900"
     CDS             complement(662..2074)
                     /locus_tag="PKX82_09900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOF52609.1"
                     /translation="MHALSPTDSAFMWLETRSQPMHVAGLNIYTPPKDSSHDYVSALL
                     ADWGKHLSAQAPFNQRPVMKMGLWYWDEDKDFELDYHLRHLALPQPGRIRELLAMVSR
                     LHGNLMDRNRPLWECYVIEGLPGGRFAIYTKIHHALIDGVTGARMMVESLSPTPQGYK
                     PPLWACEFDKHPTSRRNTRPRSLLQQITDLAKAGREILPGIGGGLWDMVRAQVEETAQ
                     AMPFQAPPTPFNVEIAGARRFASQSYSLARFKKIGAASGATVNDVTLAVCAGALRKYL
                     EMQKALPRKPLVAMVPVSLHGETDEGGNQVSLLLANLGTHIKDPLKRLQSIVKSTAEA
                     KKRLSSMPRLQKMAHGATSISGMGPAMVTGAARKHPLFNVVISNVPGPRDTLYLDGSR
                     LDEVYPVSIPTHYLALNITISGYGDNLGFGYIACRRSVPSLQRMLDFTDQSIEELEAA
                     VGLEAAPKKRAPRKASSKTA"
     gene            complement(2105..>3265)
                     /locus_tag="PKX82_09905"
     CDS             complement(2105..>3265)
                     /locus_tag="PKX82_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOF52610.1"
                     /translation="KAEKSKDFNDLGMPASEVTMAEALKARGYHTLHIGKWHLGSTPE
                     MRPNNQGFDETLFMESGLHLPVNSPDVENSRQDFDPIDKFLWPNMRFGVSYNGGKWFE
                     PAKYLADYYTDEAVTAIHKNRNRPFFLYLAHWGVHTPLQASKADYDALSSIPDHRRRV
                     YMAMVKSIDRSVGRVLQTLKEEGLDDNTIVIFASDNGAPNYIGLPDVNSPYRGWKLTM
                     FEGGLRVPYMAKWPGHIAPGTQYKAPITNIDILPTVVAAAGGTLPQDRPIDGVNLLPY
                     LGAKPAAQPARFLFWRDGPYRAMRDATWKLIVSERPKKEWLFNLASDPTEKTNLATEQ
                     PQKLAELKSLMDAHHAQMPPSMWPSFIEMVIEIDKTLNDKHLPTDEYVYWYN"
BASE COUNT          569 a          936 c         1084 g          676 t
ORIGIN      
        1 cggcagcggt cagtgtcagg ctcatggctg ctggctccgt gcggccacct ggcccagcag
       61 gcggtaggtc caggcgcagg ctgcaccgcc cacgaccaga agcacccagg ccaggggaat
      121 tcgcagcaat ccaatcgcta caaatgtaat agctgcgaac gcccagcaga tgggggctcc
      181 catcggattt tcctttaagg cagcaatcag cttgagcccg gtggcagtga tcaggccggc
      241 cgcgaccgcc cccatgccac gcagtgcgcc ctgcacctgg ggctggtcgg cgatgccggc
      301 aaacaggatg gccacggcca gtgcaatcac cagcgggaag gtcaacatgc cagccagtgc
      361 agccagtgcg ccgcgcatgc cgaagtagcg gtcgccgatc atcagcgaga ggttgaccac
      421 attggggcct ggcaggatct gggcgacggc ccagtcttcc agaaactctt ccagcgtcag
      481 ccatttcttt ttttccacca gctcacgctg tactacggcc agcacaccgc caaacccttg
      541 cagggccaac agggagaagg agacaaacag gtcggttttg gaggtggggt gggcgcgggt
      601 ggcaggtggt gcggacacgg gtaactcggt cggggaagtg caggggaggc tgcactgtac
      661 ctcaggcggt cttgctgctg gccttgcggg gtgcccgttt tttgggggcc gcctccaagc
      721 ctacggccgc ttccagttct tcaatggatt ggtcggtgaa gtccagcatg cgctgcagtg
      781 acggcaccga gcgccggcag gcaatgtagc caaaaccgag gttgtcgccg taaccgctga
      841 tggtgatgtt cagcgccagg taatgcgtgg gaatggacac ggggtagacc tcgtccaggc
      901 gggagccatc caggtacagg gtgtcgcgcg gaccgggcac attggagatg accacattga
      961 acagcgggtg cttccgtgcc gcgcccgtca ccatggcggg ccccatgccg gaaatggacg
     1021 ttgcgccatg cgccatcttc tgcagccgtg gcatgctgga gagccgtttc ttggcttcgg
     1081 cagtggactt gacaatgctt tgcaggcgtt tgagtgggtc cttgatatgg gttcccagat
     1141 tggccagcag caacgacacc tggttgccac cctcatccgt ctcaccgtgc agggacactg
     1201 gcaccatggc gaccagcggt ttcctgggca gggccttctg catttccagg tatttgcgca
     1261 gtgcgccggc gcacacggcc agcgtgacat cgttcacggt ggcacccgaa gccgcaccaa
     1321 ttttcttgaa acgggccagg gaatacgact gggacgcaaa ccggcgtgca ccggcaattt
     1381 cgacattgaa cggggtgggc ggtgcctgga acggcatggc ctgcgcggtt tcctccactt
     1441 gcgcacgcac catgtcccac aagccgccgc caatgcccgg caggatctcg cggccggcct
     1501 tggccaggtc ggtgatctgc tgcagcaggc tacgcggccg tgtgttgcgc ctggaggtgg
     1561 ggtgcttgtc aaattcgcag gcccacagcg ggggcttgta gccctgcggt gtgggcgaca
     1621 gactctccac catcatgcgg gcacccgtga cgccatcgat cagcgcgtgg tggatcttgg
     1681 tgtagatggc aaaacggccg cccggaagtc cttcgatcac atagcactcc caaagcgggc
     1741 ggttgcggtc catcaggttg ccgtgcaggc gtgacaccat ggccagcagt tcccggatcc
     1801 gtccgggttg tggcagcgcc aggtggcgca ggtggtagtc gagttcgaaa tccttgtctt
     1861 cgtcccagta ccacaggccc attttcatga ccgggcgctg gttgaacggt gcctgggcgg
     1921 agaggtgttt gccccaatcg gccagcaggg cgctcacata gtcgtggctg gaatccttgg
     1981 gcggggtgta gatgttcagc cctgccacat gcatcggctg gctgcgggtt tcgagccaca
     2041 tgaaagccga atcggtaggg ctgagtgcgt gcatgcgttg tctcctgata ttgcgcattg
     2101 taggtcagtt gtaccagtat acgtactcgt ctgtcggcag gtgcttgtcg ttcagggtct
     2161 tgtcaatctc gatgaccatt tcgataaagg agggccacat cgacggcggc atctgcgcgt
     2221 ggtgtgcgtc catcagcgac ttcagttctg ccagcttttg gggctgctcg gttgccagat
     2281 tggtcttctc ggtggggtcg ctggccagat tgaacagcca ttcttttttg ggacgctcgg
     2341 acacgatgag tttccaggtg gcatcgcgca tggcccggta gggaccatcg cgccagaaca
     2401 ggaaacgggc tggctgcgct gcaggcttgg ctcccaggta gggcagcagg ttgacaccgt
     2461 cgatggggcg atcctgcggc agggtgccgc ccgcagccgc gaccacggtg ggcaggatgt
     2521 cgatgttggt gatgggcgcc ttgtactgcg tgcctggggc aatatgtcca ggccatttgg
     2581 ccatgtaggg cacgcgcagg ccaccctcaa acatggtgag cttccagcca cggtaggggc
     2641 tgttcacatc cggcagcccg atgtagttgg gtgcaccatt gtcactggcg aaaatcacga
     2701 tggtgttgtc atcgaggcct tcttctttca gggtttgcag tacccggccc acactgcggt
     2761 cgatggactt gaccatggcc atgtagacgc ggcggcggtg gtcggggatg ctgctcagcg
     2821 catcgtaatc ggccttgctg gcctgcagcg gcgtgtgcac gccccagtgg gccaggtaca
     2881 ggaaaaatgg ccggttgcgg ttcttgtgga tggcggtgac cgcttcgtcg gtgtaatagt
     2941 ccgccaggta tttggcgggt tcgaaccatt tgcctccgtt gtagctcacg ccaaaacgca
     3001 tgttgggcca caggaacttg tcgatcgggt cgaagtcctg tctggagttc tccacgtcgg
     3061 gtgaattcac gggcaggtgc agtccgctct ccatgaacag ggtttcgtcg aagccctggt
     3121 tgttggggcg catttcgggg gtactgccca gatgccactt gccgatatgc agcgtgtggt
     3181 acccgcgtgc tttcagggct tcggccatgg ttacctcgga cgcgggcatg cccagatcgt
     3241 tgaagtcctt ggatttttcg gcctt
//
LOCUS       DAOSJM010000284         3264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25661_length_3264_cov_4.014023, whole genome shotgun sequence.
ACCESSION   DAOSJM010000284 DAOSJM010000000
VERSION     DAOSJM010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25661_length_3264_cov_4.014023"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..537)
                     /locus_tag="PKX82_09910"
     CDS             complement(127..537)
                     /locus_tag="PKX82_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOF52611.1"
                     /translation="MKFASVRLVTGDPASVDTLVSFYTRLTGVAATRPVPDFAELRLA
                     GLVLAISTERMIQQFNAGAARAAANRSAILEFEVDDVDALHQRLGEATLDTAMPPTTM
                     PWGNRSMLLRDPDGNVVNVFSRPTVNPQEAPQAA"
     gene            complement(582..1331)
                     /locus_tag="PKX82_09915"
     CDS             complement(582..1331)
                     /locus_tag="PKX82_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HOF52612.1"
                     /translation="MSRSARLFDLLHILRSHRRAVSGQALAQQLGISIRTLYRDIASL
                     QAQGAKIEGEPGVGYMLKPGFVLPPLMFTPEEVESLVLGARWVAQQTDAGLAAAARSA
                     LARIAAVLPPEQRHDLEASALLVATRQRDPSGQSADHEPLTVTVAPELLRKAVRTERK
                     LHITYHDAQGAQSRRTVWPFALAYFEHARVLMCWCELRQDFRYFRTDRIASAEMLDAR
                     YPQRKQALLKAWRLTLTPEKRFTILPETDSL"
     gene            1426..1992
                     /locus_tag="PKX82_09920"
     CDS             1426..1992
                     /locus_tag="PKX82_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HOF52613.1"
                     /translation="MLLCLPLPGLVWAQPTAPEPEDAPQAQPHYNVSAAQLQAGVAQR
                     FPMRFPVQGLMNLDVQVPQLRLLPAVNRLGAVMDVVAAGPALERTHQGTLDVEFALRY
                     EATDRTVRAHQLRFKRLLFPTLQPGVVALLNTYGPALAQQTLLEVVVHTLRPQDLALP
                     DALGMQPGSMTITTEGLTIGFVPKGRAG"
     gene            complement(2044..2349)
                     /locus_tag="PKX82_09925"
     CDS             complement(2044..2349)
                     /locus_tag="PKX82_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOF52614.1"
                     /translation="MTRIELAPEVLDDFDRFFEHIAQFDAGSAPQRIGEILDAIQILT
                     HSPLIGRPVKDGKRELVIGRATRGYVALYRYVAAIDTVFVLAVRAQREDGFKHENGL"
     gene            complement(2346..2615)
                     /locus_tag="PKX82_09930"
     CDS             complement(2346..2615)
                     /locus_tag="PKX82_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOF52615.1"
                     /translation="MSTTTIRIEDDLKARVAAAAQRAGKTAHAFILDAISQTVEQVEL
                     DNAFNAVADQRWAAIQAGGKTVAWDDAKAYLAARANGEPTRKPGA"
     gene            complement(2734..>3264)
                     /gene="alkB"
                     /locus_tag="PKX82_09935"
     CDS             complement(2734..>3264)
                     /gene="alkB"
                     /locus_tag="PKX82_09935"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HOF52616.1"
                     /translation="EAVIAQAPLRHMQTPGGYTMSVATTSCGQLGWVSDPHGYRYAAH
                     DPHTGQPWPAMPACFMELAESAAAQAGYADFVPDACLINQYAPGAKLSLHQDKDERDL
                     RAPIVSLSLGLPAVFLFGTPNRKDRTQRHRLVHGDVVVWGGPSRLAYHGVMPLAEGEH
                     GLLGRRRVNLTFRRAG"
BASE COUNT          558 a         1037 c         1090 g          579 t
ORIGIN      
        1 caaatagtgt tttcattgcg aactcttttg gtagaaatag ggcgggtggc ctattttgca
       61 cacagctcgc acccggtgct tcgcccacca ccctccagca cgccgagtca gggggtagcg
      121 ccaaactcat gcagcttggg gggcctcttg cgggttgacc gtggggcggg aaaacacgtt
      181 cactacattg ccatccgggt cgcgcagcag catggagcgg ttgccccagg gcatggtggt
      241 gggtggcatg gcagtgtcca gggtggcttc gcccaatcgc tggtgcaggg catccacatc
      301 gtccacttca aattcgagga tggctgaccg gttggccgca gcgcgggctg cccccgcgtt
      361 gaactgctgg atcatgcgct cggtggagat ggcgagtacc aggcccgcga ggcgcagctc
      421 cgcaaagtct ggcaccggcc gtgtggcggc cacacccgtc aggcgcgtat agaaggacac
      481 cagcgtgtcg acactggcag ggtcgccggt aacaaggcga acggaggcaa atttcatcag
      541 gggctcctgg gtgttaagaa aagaaggagc caagactagg gttagaggct gtcagtttct
      601 ggcagtatgg tgaagcgctt ctcgggcgtg agcgtgagcc gccaggcctt gagcagcgcc
      661 tgtttgcgct gggggtagcg ggcatccagc atctcggcgc tggcaatacg gtcggtgcgg
      721 aagtagcgaa aatcctgccg cagctcgcac cagcacatga gcacgcgcgc gtgctcgaag
      781 taggctaggg caaagggcca gacagtgcgc cgggactgcg caccctgggc atcgtggtag
      841 gtgatgtgca gcttgcgctc agtgcgcacg gccttgcgca ggagctcggg ggccacagtc
      901 acggtcagcg gttcgtggtc tgcgctctgg ccactcgggt ctctctgcct ggtggccacc
      961 agcaaggccg atgcctccag gtcatggcgc tgctcagggg gcagcaccgc cgcaatgcgt
     1021 gccaacgcgc tgcgtgccgc agcggccaaa ccagcatcgg tctgctgcgc cacccagcgc
     1081 gcacccaaca ccagcgattc gacttcttcg ggcgtgaaca tcagcggggg cagcacgaac
     1141 ccgggtttga gcatgtagcc cacgcccggc tccccttcga tctttgcgcc ctgtgcctgc
     1201 aggctggcaa tgtcgcggta cagcgtgcga atgctgatgc ccaactgctg cgccagcgcc
     1261 tggccactca ccgcgcgccg gtggctacgc aggatgtgga gcaggtcaaa aaggcgggcc
     1321 gaacgggaca tgcgacggat gatacggccg cactggcata ccatccccag gtaccgataa
     1381 cccaaccgct gagcaactga agtgcaccgc cgctttttac tccccatgct tctctgcttg
     1441 ccactgcccg gcttggtgtg ggcgcagccc accgcccccg agcctgaaga cgcgccccag
     1501 gcgcagccgc actacaacgt gtcggccgca cagttgcagg cgggcgtggc gcagcggttc
     1561 cccatgcgct ttccggtgca ggggctgatg aacctggatg tacaggtgcc gcagctgcgc
     1621 ctgctgccgg cggtcaaccg cctgggcgcg gtgatggacg tggtagccgc gggcccggcg
     1681 ctggagcgca cgcaccaggg cacgctggac gtggagtttg cgctgcgcta cgaggccacc
     1741 gaccgcacgg tgcgcgcgca ccagttgcgc ttcaagcgcc tgctgttccc caccctgcag
     1801 ccgggcgtgg tggcgctgct caacacctac ggcccggccc tggcccagca gacgctgctg
     1861 gaggtggtgg tgcacaccct gcgcccgcaa gacctggcgc tgcccgatgc gctgggcatg
     1921 caacccggca gcatgaccat caccactgaa gggctgacga ttggctttgt gcccaaaggg
     1981 cgggcgggct aatccgtacg gttgctatag attcaatagc tgcttgcgca tattccacgg
     2041 gggctacagg ccgttttcat gcttaaaccc atcctcacgc tgtgcccgca ccgccagcac
     2101 aaacaccgtg tcgattgccg ccacatagcg gtacagcgcc acatagccac gcgtggcgcg
     2161 gccaatgacg agttcgcgtt tgccgtcttt cacgggccgg ccgatcaggg ggctgtgggt
     2221 caggatctgg atggcgtcca gtatttcgcc aatgcgctgg ggcgcagagc cagcgtcgaa
     2281 ctgggcgatg tgctcaaaga agcggtcgaa gtcgtccaag acctccggcg ccaactcaat
     2341 gcgggtcatg cgccgggctt gcgggtgggc tcgccattcg cccgcgcggc gaggtaggct
     2401 tttgcgtcgt cccacgccac ggtcttgccg ccggcctgaa tggcggccca gcgctggtcg
     2461 gccactgcat taaaggcgtt gtccagttcc acctgctcta cggtttgcga gatggcatcc
     2521 agaatgaaag cgtgggcagt tttgccagcg cgctgtgcgg cagcggccac gcgggctttg
     2581 aggtcgtctt caatgcggat ggtggtggtg gacatgtgcg ctcccctgat ggatggtaaa
     2641 tagtagcact ttggtgctac atatgcctgc ccagcacctc tgctgagccg catttcccaa
     2701 aatacgcctc attttttgag ccgtattgtt gaattacccc gcccgccgaa acgtcaggtt
     2761 cacccgccgc cgccccagca acccatgttc cccctcggcc agcggcatca caccgtggta
     2821 cgccaggcgc gatgggccac cccacaccac cacgtcgcca tgcaccagcc gatggcgctg
     2881 ggtgcggtct ttgcggttgg gggtgccaaa cagaaacacc gcgggcaggc cgagtgacag
     2941 cgacacgatg ggcgcgcgca gatcgcgttc gtccttgtcc tggtgcagcg agagcttggc
     3001 gccgggggcg tactggttga tcaggcaggc gtcgggcaca aagtccgcat agccggcctg
     3061 cgctgcggcg ctttcagcca gctccatgaa gcacgcgggc atggcgggcc agggctggcc
     3121 cgtgtgcggg tcgtgcgcgg catagcggta gccatgcggg tcagacaccc agcccagctg
     3181 cccgcagctg gtggtggcca ccgacatggt gtagccgccg ggggtttgca tgtggcgcag
     3241 cggtgcctgg gcgatgacgg cctc
//
LOCUS       DAOSJM010000285         3255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25755_length_3255_cov_4.224687, whole genome shotgun sequence.
ACCESSION   DAOSJM010000285 DAOSJM010000000
VERSION     DAOSJM010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25755_length_3255_cov_4.224687"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PKX82_09940"
     CDS             complement(<1..833)
                     /locus_tag="PKX82_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOF52617.1"
                     /translation="MPNAIAPQTEIRLRTRPISVGHLRALEAVARHLNFRAAAEDLAL
                     TQSAVSRQVQALEDEVGVPLFLRHTRAVELTSAGNQLLRAAVPSLERIDSAVRLIRQS
                     SGRKSVAISTWASFASMWLIPRLEAFQAEYPDIDIRIDATDNPVDLETSDVDLAIRYT
                     RPGHVGNEAVRLFGEQLTPVASPWLLKSGPPLRSPKDLANYALLEASDSHRTQNLEWL
                     TWRRWLEDNWQPKLAPKRWMHFNYAHQIVQAALTGQGIALARMPLVADSLASGDLVEV
                     LP"
     gene            946..1380
                     /locus_tag="PKX82_09945"
     CDS             946..1380
                     /locus_tag="PKX82_09945"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HOF52618.1"
                     /translation="MQALSPALSPSLSQPLQASWALRSGQALSLRPRAAGVFRVTQGR
                     AWATLDVDRRSPASDTGDHFVMPGQDLVLRAGQRVVLEAWPVEGDDAIALQWMPAAQQ
                     GSGSRWHASVVEPLHDLGRGLALVVRASLRLVRGLAGYAAAP"
     gene            complement(1390..>3255)
                     /locus_tag="PKX82_09950"
     CDS             complement(1390..>3255)
                     /locus_tag="PKX82_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOF52619.1"
                     /translation="NQTVMHAIDNRSPVDIEYRILLSDGTVKWVHLASSFVSEDAQGQ
                     IRNGVLLDITARKLAEAQLMESEARWKLALESTGDGVWDWYIQTGDEFFSRRCKEIYG
                     FAEDEIPDRSEALDGRTHPDDLPQMLRDRDAHFSGETPSYTNEHRILCKDGSWKWVLT
                     RGMVISRDANGKPLRMIGTHTDISDRKEAEAMVWRQAHFDPLTGLPNRRTLRERLHNA
                     IRHNQQTGEPVAVLFIDLDHFKEVNDTLGHDIGDLLLVEAARRITQPLGPQDTVARMG
                     GDEFTVILGQVPDAPALDAMLQGMLDRLASAFHIGPEQVFVSASMGVALYPQDATLVE
                     DLLKHADQALYVAKGAGRNRFSFFTPELQQAAQTRLRLAADLRLALAAHQFEVVYQPI
                     VQLATGAIHKAEALLRWHHPQRGLVSPAQFIGIAESTGLIIDIGDWVFRQAAAQVQAW
                     RAAHHPDFQISVNKSPIQFQATSGTPWSTVLAQMGLPGSSIAVEITEGLLLDATNAAL
                     QQLQAHGDAGMQISLDDFGTGYSSLTYLQRFAIDYIKIDQTFVRNLHAGSTDLALCKA
                     IILMAHELGMQVVAEGVETEAQRDLLVVAGCVYGQGYLFARPMSAADFEKTCLRS"
BASE COUNT          548 a         1003 c         1085 g          619 t
ORIGIN      
        1 ggcagtactt ccaccaggtc gccgctggcc agactgtcgg ccaccagggg catgcgggcc
       61 agtgcgatgc cctggccggt aagtgcggcc tggacgatct ggtgcgcgta gttgaagtgc
      121 atccagcgtt tgggcgcgag cttgggctgc cagttgtctt ccagccagcg ccgccaggtc
      181 agccactcca ggttttgcgt gcggtgggag tcactggctt ccagtagcgc atagttggca
      241 aggtctttgg ggctgcgcag cggtggcccg cttttgagca gccaggggct ggccaccggg
      301 gtgagctgct cgccaaacag gcgcaccgcc tcgttgccca catggcccgg gcgggtgtag
      361 cgaatggcca ggtccacatc gctggtctcc aggtcgacgg gattgtcggt ggcatcaatg
      421 cggatatcga tgtcggggta ctcggcctga aatgcctcca gccgcgggat cagccacatc
      481 gacgcaaacg aggcccaggt ggaaatggcc acactcttgc gcccggaact ctggcgaatc
      541 agccgcaccg cgctgtcaat gcgttccagc gaaggcacag cggcgcgcag caactggttg
      601 cccgcactgg tcagctccac cgcacgggtg tggcgcagga aaaggggcac accgacctcg
      661 tcttccagtg cctgaacctg gcggctgacc gcactttggg tcagtgccag gtcttcggcg
      721 gcggcacgga agttgagatg gcgggcaacg gcttccagtg cgcgcaggtg ccccaccgag
      781 atcggtcggg tgcgcaggcg gatttcggtc tggggcgcaa tggcgttggg catggtgcgt
      841 ggcgattgat gcggtaatgg aatgaatagc atagcccgat ttcattggac tggggtggct
      901 tccgtagcga tcatgaagtg cccatccacc caacaggagt tcaccatgca agccctcagc
      961 cccgcgctat ccccatcact ttcccagccg ctccaagcct cctgggcgct gcgcagcggt
     1021 caggccttga gcctgcgccc ccgcgctgcg ggcgtgtttc gggtgaccca ggggcgcgcc
     1081 tgggccacac tggacgtgga tcgcaggagt cccgcgtcgg acacgggcga ccactttgtc
     1141 atgccggggc aggacctggt gttgcgcgcc gggcagcggg tggtgctgga ggcgtggccc
     1201 gtggaagggg atgatgcgat tgcgctgcag tggatgcctg ccgcccaaca gggttcgggc
     1261 agccgctggc acgcgagcgt agtggagccg ctgcatgatc tggggcgcgg cctggcgttg
     1321 gtggtgcgtg caagcttgcg gctggttcgt gggctggccg ggtatgccgc cgcaccctga
     1381 gaaacggcat caggaacgca agcaggtttt ctcaaaatcc gcagccgaca tgggccgggc
     1441 aaacagatag ccctgcccat agacgcagcc cgcaaccacc agcaggtccc gctgcgcctc
     1501 ggtttctacg ccctcggcca ccacctgcat gcccagctca tgggccatca ggatgatggc
     1561 cttgcacagc gccagatcgg tggagcctgc atgcaggttg cgcacgaagg tctggtcgat
     1621 cttgatgtag tcaatcgcaa agcgctgcag gtaggtcagt gatgaatagc cggtaccaaa
     1681 atcgtccagc gatatctgca tgcccgcatc gccatgcgcc tgcagttgct gcagggctgc
     1741 gttggtggca tcgagcaaca gcccctcggt aatctccaca gcaatgctgc ttccaggcag
     1801 ccccatctgc gccagcacgg tgctccaggg tgtgccggac gtggcctgga actggatggg
     1861 cgatttgttc acactgatct ggaagtcggg gtggtgtgcg gcacgccagg cctgcacctg
     1921 cgcggctgcc tggcgaaaga cccagtcgcc aatgtcgatg atcagcccgg tggattccgc
     1981 aatgccaatg aactgtgcgg gactgaccag cccacgctgc ggatggtgcc agcgcagcag
     2041 ggcttctgct ttgtggatgg cgccggtggc caactgcacg atgggctggt acaccacttc
     2101 gaactggtgg gcggctagcg ccagccgcag gtcggcggcc agtcgcagac gggtttgtgc
     2161 ggcctgctgc aattcggggg taaaaaaact gaagcggttg cgccccgccc ccttggccac
     2221 atagagcgcc tggtccgcgt gtttcaaaag gtcttccacc agggtggcat cctgcgggta
     2281 cagggccacg cccatgctgg ctgacacaaa cacctgttcg gggccgatgt ggaaggccga
     2341 cgccagccgg tccagcatgc cctgcaacat tgcgtccagc gctggcgcat ccggcacctg
     2401 gcccagaatc acggtgaact catctccccc catgcgggcc accgtgtcct gtggacccag
     2461 cggttgtgtg atgcggcgcg cagcttccac gagcagcagg tccccgatgt cgtggcccag
     2521 ggtgtcattg acttccttga agtggtccag gtcgatgaac agcacggcca cgggctctcc
     2581 ggtctgctgg ttgtgccgga tggcgttgtg caggcgttcg cgcaaagtcc ggcgattggg
     2641 caggccggtc agcgggtcaa agtgcgcctg gcgccagacc atggcctcgg cctccttgcg
     2701 gtccgagata tcggtgtggg tgccgatcat gcgcaggggc tttccgttgg cgtcgcggct
     2761 gatcaccatg ccacgggtca gcacccattt ccacgatccg tccttgcaca ggatgcggtg
     2821 ctcgttggtg tagctgggcg tctcgccgct gaaatgggcg tcgcggtcac gcagcatttg
     2881 cggcaagtcg tcggggtggg tgcggccgtc cagtgcttca gaacggtcag gaatctcatc
     2941 ctccgcaaag ccgtagattt ccttgcaccg tcgggaaaaa aattcgtcgc cggtctggat
     3001 gtaccagtcc cacacaccgt ctcccgtgct ctccagtgcc agcttccagc gtgcctcgct
     3061 ctccatcaac tgggcttcgg ccaatttgcg ggccgtgatg tcgagcaaga cgccattgcg
     3121 tatctggccc tgtgcgtctt ccgatacaaa cgatgacgcc agatgcaccc acttgacggt
     3181 gccgtcactc aacaggatgc ggtactcgat gtcaaccgga ctccggttgt cgatggcgtg
     3241 catcacggtt tggtt
//
LOCUS       DAOSJM010000286         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25961_length_3234_cov_6.106952, whole genome shotgun sequence.
ACCESSION   DAOSJM010000286 DAOSJM010000000
VERSION     DAOSJM010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25961_length_3234_cov_6.106952"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PKX82_09955"
     CDS             complement(<1..366)
                     /locus_tag="PKX82_09955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF52620.1"
                     /translation="MTYDAGAQMLEIAGTVFTLRDVGPSDCTAVLGLHRRVFGSTVDA
                     AWFDWKYRHGGGEAVGLWQGNELVAHCGGTPRTVLHHGRPARDLQIGDVMVAPEWRGV
                     LRRRGPFYHVSERLYSSRLG"
     gene            complement(379..1617)
                     /locus_tag="PKX82_09960"
     CDS             complement(379..1617)
                     /locus_tag="PKX82_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF52621.1"
                     /translation="MVAPASQAQAYAIEARSLGKCYGLYAHPSDRLKQLLWGRWRQFS
                     REFWALREVDLLIRPGEVVGLVGRNGAGKSTLLQMLCGTLPPTTGRLQVHGRVAALLE
                     LGAGFNPEFTGIENVFMNGAILGLKHHEVEERLDEILAFADIGEFVHQPVKTYSSGMF
                     VRLAFAVATSVEPDILVIDEALAVGDGAFARKSFDRIMRLKDSGKTILFCSHSMYQVE
                     ALCSRAIWIDKGKLRLQGSAAEVTSAYNASLASGGGFASQAASYAPPGQMRTAVPEGT
                     GRIVRAVARTEYEEGRQVHVHSSETDVAITIEFAIDPSLPVPGVAVGFSDATGLTVAS
                     AGSVNDGVTLRMDSDGRGQATLLFRRLPLLKGRYSVTCFLTTEDGVHPYDQVEQCLQL
                     EVTQHGLEQGVVTLPHEWQA"
     gene            complement(1628..2431)
                     /locus_tag="PKX82_09965"
     CDS             complement(1628..2431)
                     /locus_tag="PKX82_09965"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF52622.1"
                     /translation="MSILLSYLFPLRHAGLIGQLARREVHTRYRQSWLGTAWLVLTPL
                     LMLVVYTLVFRHVFQVRWSPPGEGNLAFALRLYAGLAVFNFFAECVNRAPLLIVEQPH
                     LVKKVVFPVEVLPWASVMAALAHLGIAAVALLGLRWLGMGTVPLSALALPLVWLPLLP
                     LCLGLGWGLSAVGTFIRDIGQILSMAMTALVFMSPVFFPVDALPGGLRDWMWLNPLAP
                     IMTQTRTVLLEGLWPDWSLCSLSFVASLAVAWAGAALFRRLRPGFSDVV"
     gene            complement(2503..>3234)
                     /locus_tag="PKX82_09970"
     CDS             complement(2503..>3234)
                     /locus_tag="PKX82_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF52623.1"
                     /translation="RPTLNLRRMPTSLQLISQFPLLQPLPRETLESLSGQMTLRSFAR
                     RAMVMSKDSPAQELGFLVDGRLQGVDFTVDGRSVGLYFVDPGDYFGELSVVDSQPLSE
                     YVMAAVKSTVAFLDSRTARQLILNHPELARAVMTRLAQRVRSVMAQRTLLSLPNPIQR
                     LCVLLLQLPRRAADGNLPQATVDQAPTHQELAIMINASRETVTRSFQLLFVNRIVERD
                     GHALRVLRPEMLKEIAEGRVDPPKA"
BASE COUNT          728 a         1100 c          892 g          514 t
ORIGIN      
        1 gcccagccgg ctggagtaaa gccgttcgga cacgtgataa aaaggcccgc ggcgcctcag
       61 gacaccccgc cactcgggcg ccaccatcac atcgccgatc tgcaggtcac gtgccggtcg
      121 gccatgatgc agcacggtgc gcggcgtgcc accgcagtgc gccaccagtt cattgccttg
      181 ccacagaccc accgcctcgc cgccgccatg gcggtacttc cagtcaaacc aggctgcatc
      241 cactgtcgaa ccaaaaacac ggcggtgcag gcccaagaca gctgtgcaat cggatgggcc
      301 gacatcccga agtgtaaaaa ccgttccagc gatctcgagc atctgggctc cggcgtcgta
      361 tgtcatgggc tgacggtttt aagcctgcca ctcatggggc aaggtcacca cgccctgctc
      421 caggccgtgt tgggtcactt ccagttgcag acactgttcc acctgatcgt atgggtgcac
      481 gccgtcctcg gtggtcagaa agcaggtcac cgaatagcgc cccttcagca aaggcagccg
      541 cctgaacaag agggtcgcct gtccgcgtcc atcgctgtcc atacgcagcg tcacaccgtc
      601 attgaccgag ccggcgctgg cgacggtcaa ccccgtcgca tcggagaaac ccaccgccac
      661 gccgggaacc ggcagggacg gatcaatggc gaactcgatg gtgattgcga catcggtctc
      721 gctcgagtgc acatgcacct gccggccctc ctcgtactcg gtgcgggcca cggcacgaac
      781 aattcgcccg gtaccctcag gaacagctgt gcgcatctgc ccaggcgggg cgtacgatgc
      841 ggcttgcgac gcaaagccgc ccccggaagc caggctggcg ttgtaggcgc tggttacttc
      901 cgctgccgag ccttgcaacc gcagtttgcc cttgtcgatc cagatcgcac gtgagcacaa
      961 ggcctccacc tggtacatcg aatgcgagca aaacaagatc gtcttgcctg aatccttcag
     1021 ccgcatgatc cggtcaaacg atttgcgcgc aaatgcaccg tcccccaccg ccagcgcttc
     1081 gtcaataacc agaatatcgg gctccacgct cgtggccacc gcaaaagcca atcggacgaa
     1141 catcccgctc gaataggtct tcacaggttg atggacaaac tccccgatat cggcaaatgc
     1201 cagtatttca tccagccgct cctccacctc gtgatgcttc agtccgagaa tggcgccatt
     1261 catgaagaca ttctcgatgc cggtgaactc cgggttgaac cccgccccca gttccagcag
     1321 ggccgccacc ctgccgtgca cctgcagacg gcccgtggtc ggtggcaggg tgccgcacag
     1381 catctgaagc aaggtcgact tgccggcgcc gttgcgcccc accagcccca ccacttcgcc
     1441 gggcctgatc agcagatcca cttcgcgcaa tgcccagaac tcgcggctga actgtcgcca
     1501 ccgtccccac agcaattgct tcagtcggtc actggggtgg gcatacaaac cgtagcactt
     1561 gcccagactg cgggcctcga tcgcataagc ctgcgcttgc gaggcggggg ccaccatgga
     1621 ggccgattca gacaacatcg gaaaacccgg gcctgagccg acggaaaagg gcggcgcccg
     1681 cccaagccac cgccagactg gcaacgaaac tcaaggagca gaggctccaa tccggccaca
     1741 gcccctccaa tagcaccgtg cgtgtctggg tcatgatcgg tgccagcggg ttcagccaca
     1801 tccagtcacg caggccaccc ggcaaggcat ccacgggaaa aaacaccggg ctcatgaaga
     1861 ccagcgccgt catcgccatc gacaatatct ggccgatatc acgtataaaa gtgcccactg
     1921 cgctcagacc ccagcccaag cccaggcaaa gtggcagcaa gggcaaccac accaggggca
     1981 aggccagcgc cgacaacggc accgtcccca tccccagcca ccgcaggcct agcagcgcta
     2041 ccgctgcgat gcccaaatgc gccagtgcag ccatcacact ggcccagggg aggacctcca
     2101 ccgggaacac cactttcttc accagatgcg gttgttccac gatcagcagc ggtgcccggt
     2161 tcacacactc ggcaaagaaa ttgaacacgg caaggcccgc atacaggcgc aacgcaaagg
     2221 ccagattccc ctcgcccggg gggctccaac ggacttgaaa cacatgccgg aacaccaggg
     2281 tatagaccac cagcatcaac agcggtgtca gcaccagcca ggccgtaccc agccaggact
     2341 ggcggtagcg cgtgtgcacc tcccgtcggg ccaactggcc aatgaggccg gcgtggcgca
     2401 acggaaacag gtacgacaaa aggatactca aaccaccaga cccacgaaaa tgaagaccaa
     2461 gggcaaatct gaaccacagt acctgagcgt gcggccagcg gctcaggctt tcggaggatc
     2521 gacgcggcct tccgcaattt ccttcagcat ctcgggtcgc aagacgcgca acgcatgccc
     2581 atcgcgttcc acgattctgt tgacgaacaa gagctggaaa gagcgcgtca ccgtctcgcg
     2641 actcgcattg atcataatcg cgagttcctg gtgcgtcgga gcctgatcga ccgtagcctg
     2701 cggcaagttc ccatctgcag cgcgcctcgg caattgtagt agcaacacgc aaagcctctg
     2761 aatgggattg ggcaggctga gcaatgtgcg ctgcgccatg accgaacgca cgcgttgcgc
     2821 cagccgtgtc atcaccgcgc gggcaagttc ggggtggttc aggatcagct ggcgtgccgt
     2881 tcgcgaatcc agaaaagcca ccgtcgattt gacggcggcc atgacgtatt ccgacagagg
     2941 ctggctgtcc accacggaca actcgccgaa atagtccccc ggatccacaa aatacaagcc
     3001 cacgctgcgt ccatccaccg tgaaatccac accttgtagc cgtccatcca ccaggaatcc
     3061 caactcctgg gcaggactgt cctttgacat caccatcgca cgacgcgcaa aagaccgcaa
     3121 agtcatctgg cctgaaagcg attccagcgt ctcccgcggc agcggttgca gcagcggaaa
     3181 ctgagaaatc aattgaagcg aagtcggcat gcgcctcaag tttagcgttg gcct
//
LOCUS       DAOSJM010000287         3233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_25976_length_3233_cov_4.098490, whole genome shotgun sequence.
ACCESSION   DAOSJM010000287 DAOSJM010000000
VERSION     DAOSJM010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25976_length_3233_cov_4.098490"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..706)
                     /locus_tag="PKX82_09975"
     CDS             complement(158..706)
                     /locus_tag="PKX82_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52624.1"
                     /translation="MLRGGALLSPLLGIVVILPWLWALPRLHTMPLQLQLSGACAATL
                     MLGWPLAVPVLAIVALLGGVIAPAEWDALVAQAVWQGVVPASLALAAGALLRRLAGPH
                     PFVYILGRAFAATVLCTFLAEYLSQLAGHEILNVGMSLSMVARWLVAWGDGFMTGLLA
                     AIFVAYRPQWLATWSDRLYLKS"
     gene            complement(775..1317)
                     /locus_tag="PKX82_09980"
     CDS             complement(775..1317)
                     /locus_tag="PKX82_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52625.1"
                     /translation="MPLPTSLPADATAESIVRHFQSLGFPGITEALLVRIRLKKGDRM
                     EVDAAFDVATEREAQPPVHEYFEIRLYGFFSQIRTLAEAKAAFATDFGRGLRIELPSI
                     YFDEAPVVVDDALATGTKYDAMLKLGNNTLGHAVGILLNDPSSSFFEYLGTHADYDWQ
                     KIMSNLETAASSFVPEEDLL"
     gene            1405..2400
                     /locus_tag="PKX82_09985"
     CDS             1405..2400
                     /locus_tag="PKX82_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52626.1"
                     /translation="MFSNSQQILVYLSHPLTQIVVFMAASALMIWRLQAIESKGFEGT
                     VLGTLIMPYCSGFANLVFAFVMSRQGGQGVTVLENCIVNNVTNLTLILGLTTLAFGAR
                     AASKSAKGKKKVVPPAHRIYKLELLFTLVALFLFTGTLWALAKDGTLNLYDGLVLVGL
                     FVFWQVLHVFEILKNNVRKNRGFHWSIVLDLLLIAAGAYGIYYSVNFLVDWVSTLESA
                     YVSAKDLGWLSGLLMVVPNAFIALYYGYVGRQDIVVSSQVGDGHICIPMCIGLFALSD
                     KITIPGFFQIGIYVIAGAAVLHFLCIAFLGRLPRALGYVLVAAYGVFVYTGVVQQ"
     gene            2465..>3233
                     /locus_tag="PKX82_09990"
     CDS             2465..>3233
                     /locus_tag="PKX82_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52627.1"
                     /translation="MCDTLYVRQNGVGWFAKNSDREPDEPQAVVWLPPAQDDPAQTVR
                     ATYMDIPQQPRRYGVLLSQPAWIWGGEIGVNTQGVAIGNEAVFSKQVDRKGQNLLGMD
                     LLRLGLERGGTAREALDVIVWHLERFGQGGPAGYRDKGFRYDNSFLIADASGAWVLET
                     AGRMWAARAVQSWAISNCYSLGTEFDLCSKGLQDEARRLRLWNGQGDFDFAKTFETWL
                     YPFVGGAHRRRALNCDAIAGGLVPGWASLAARLRDHGQ"
BASE COUNT          604 a          909 c         1061 g          659 t
ORIGIN      
        1 gcggcggctc tatcgggctg cggtccgggt tcggggctat cgggatgatc ttgcgattgg
       61 gggtgttgtt ggcgtccacg ggtgttcctt cgatgcgtca ataataccct tttgggtatt
      121 cctggcgcat gataaatatc aagtcagtgg agattctcta cgacttgagg taaagccggt
      181 ctgaccaggt cgcaagccac tgcggccggt aggctacaaa gatggccgca agcagcccgg
      241 tcatgaaacc atcgccccag gccaccagcc agcgggccac catcgacagg ctcatgccga
      301 cgttcaggat ttcgtgcccg gccagctggg acaggtattc cgccagaaag gtgcacagca
      361 ccgtggcggc aaaagcccgg cccagaatgt agacgaacgg gtgggggcct gccagacggc
      421 gcagcagcgc ccctgcagcc agcgccaggc tggcgggcac cacgccctgc cagaccgcct
      481 gagcgaccag cgcatcccac tcggcggggg caatcacgcc tcccagcagc gccacgatgg
      541 ccagtaccgg cacggccagt ggccacccca gcatcagtgt ggcagcgcag gcgccagaca
      601 gctgcaattg cagcggcatg gtgtgcagac ggggcagcgc ccacagccag ggcaggatca
      661 ccacaattcc cagcagcggg gagagcaggg cacccccgcg cagcatctgc caggggcgca
      721 gtgccagcgc cgcaaggagt gctgcggcga ggagggcgac ttcgaggctg gaggtcagag
      781 caaatcttct tccggaacaa atgaactggc agcggtttcc agattgctca tgatcttctg
      841 ccagtcgtag tccgcgtggg tacccaggta ttcgaaaaac gatgaactcg ggtcgttcag
      901 cagaatgccc actgcgtggc cgagggtgtt gttacccagc ttgagcatgg catcgtattt
      961 ggtgccagtg gccagcgcgt catcgacaac cactggcgcc tcgtcaaagt agatgctggg
     1021 aagctcgatg cgcaggccgc gtccgaaatc ggtggcaaat gcggctttgg cttcggcaag
     1081 cgtgcggatc tgggaaaaga agccatacag ccggatctcg aaatactcat gcaccggggg
     1141 ctgcgcctcg cgctcggtgg ccacgtcaaa ggcggcgtcc acctccatgc ggtcgccctt
     1201 cttgagccgg atgcgcacca gcagcgcttc ggtgatgccg gggaatccca gggactggaa
     1261 atggcgcacg atgctttcgg cggtggcgtc agcgggcaga ctggtcggaa gtggcatgaa
     1321 aacggatctt tcacaaaagg gcgcagtgta cctaacctgt attgcgctta tcctaggcgc
     1381 tttctcctgg cccccatcgc acacgtgttc agcaatagtc agcaaattct cgtttacctg
     1441 tcgcatccgc tgacacagat cgttgtgttt atggccgcct cggccctgat gatctggcgg
     1501 ttgcaggcga ttgagagcaa aggctttgag ggcaccgtgc taggcaccct gatcatgccg
     1561 tattgctccg ggtttgcgaa cctggtcttt gcgtttgtca tgagccggca gggtggccag
     1621 ggggtgacgg tgctggaaaa ctgcattgtc aacaatgtca ccaatctcac gctgattctg
     1681 ggcctgacca cgctggcttt tggagccaga gcggccagca aaagcgccaa aggcaagaag
     1741 aaagtcgtgc ccccggccca ccgtatttac aagctggaat tgctgttcac cctggtggca
     1801 cttttcctat tcaccggcac tttgtgggca ctggccaagg acggcacgct gaacctgtat
     1861 gacggattgg tgctggtggg tctgttcgtg ttctggcagg tcctgcatgt gttcgaaatt
     1921 ctcaagaaca atgtgcgcaa gaaccgcggg ttccactggt ccatcgtgct ggacctgctg
     1981 ctcattgcgg caggagcgta tggtatttac tacagcgtca atttcctggt ggattgggtg
     2041 tccacgctgg agagtgccta tgtcagcgca aaggacctgg gctggctcag tggtctgctg
     2101 atggtggtgc ccaatgcatt cattgcgctt tactacggct acgtgggccg gcaggacatt
     2161 gtggtgagct cgcaggtggg cgacgggcat atctgtattc ccatgtgcat tggtttgttt
     2221 gccctgtcgg acaagatcac gattccgggg tttttccaga ttggcattta cgtgatcgca
     2281 ggtgctgcgg tgctgcattt cctgtgcatt gcgttcttgg ggcgcttgcc gcgcgccctg
     2341 ggctacgtgc tggtagcggc ttacggagtc tttgtgtaca ccggcgtcgt gcagcaatag
     2401 acttctgtca gtcgggtgga ccccggacgg gctgtgtgga tgggttttag cagggagtcg
     2461 atctatgtgc gatacgcttt atgtccgcca aaacggtgtg ggctggtttg ccaagaacag
     2521 tgaccgcgag cccgatgaac cgcaggccgt ggtctggctg ccacccgcgc aggatgaccc
     2581 ggcgcagacc gtgcgggcca cctacatgga catcccgcaa caaccgcggc gctatggtgt
     2641 gctgctgtcg caaccggcat ggatttgggg cggcgagatc ggtgtgaata cccaaggcgt
     2701 agcgattggc aacgaggccg tgttcagcaa acaggtggac cgcaaaggcc agaatctgct
     2761 gggcatggat ctgctgcgcc tggggttgga gcgtggcggt accgcacgcg aggcacttga
     2821 cgtaatcgtc tggcatctgg agcgctttgg gcagggtggg cctgccggtt accgcgacaa
     2881 gggctttcgc tacgacaaca gtttcctgat tgccgatgcc agcggggcct gggtgctgga
     2941 aaccgcaggc cggatgtggg cggcacgcgc agtgcagtcg tgggcgattt ccaactgcta
     3001 ttcgctgggc accgagttcg acctgtgcag caagggactg caggacgaag cacggcgctt
     3061 gcggctgtgg aacgggcagg gcgactttga ctttgccaaa acctttgaga cctggctgta
     3121 cccgtttgtg ggcggcgcgc accggcgccg cgccttgaac tgcgatgcca ttgccggtgg
     3181 cctggtgcct ggctgggcca gcctggccgc acgtctgcgc gaccatgggc aac
//
LOCUS       DAOSJM010000288         3227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_26040_length_3227_cov_4.291614, whole genome shotgun sequence.
ACCESSION   DAOSJM010000288 DAOSJM010000000
VERSION     DAOSJM010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26040_length_3227_cov_4.291614"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..999)
                     /locus_tag="PKX82_09995"
     CDS             complement(223..999)
                     /locus_tag="PKX82_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOF52628.1"
                     /translation="MAKLVFITGASSGIGQALAWRYYLAGFSLALVARRSAEMQTWMV
                     QQNISADRCKIYSADVSDMDSIVSAGQLCLLSQGVPDVVIANAGISVGMDSAVRSDLD
                     VMARTFATNNLGLAATFHPFIDAMVQRGSGALVGIGSVAGIRGLPGHGAYCASKAAVI
                     NYCESLRGELRSSGIRVVTICPGYIDTPLTQKNRYSMPFLMQPHHFADKAFAAIAAGD
                     SYRVIPWQMGWVAKLLRLAPNVLFDKALSGRPRKRRISEP"
     gene            complement(1020..1634)
                     /gene="lptC"
                     /locus_tag="PKX82_10000"
     CDS             complement(1020..1634)
                     /gene="lptC"
                     /locus_tag="PKX82_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOF52629.1"
                     /translation="MKPVLREAWERFLLYLPLIFMGTLALLTYWLVRTTPETGGAAVA
                     RVESHDPDYFMEGFTIKTYDAKGHIRSEVVGDKARHYPDTQWLEIDAIRIRSFDEKGR
                     LTTATANRGLTNEDGSEVQLIGNALVVREADAAAGAKAIPRMEYRGEFLHAFLTTEKV
                     KSHKPVELLRGQDRFTADSLDFDNVDQILQLQGRVRGTLVPDSK"
     gene            complement(1631..2176)
                     /locus_tag="PKX82_10005"
     CDS             complement(1631..2176)
                     /locus_tag="PKX82_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOF52630.1"
                     /translation="MRPALNFDPELLLRAQGVQVAFLDVDGVLTDGGLLFSETGETLK
                     RFNTLDGHGLKMLQKVGITPVVITGRDSKPLRTRLTALGITHAHFGTEDKRPAAEQTL
                     RELGLGWESAAAMGDDWPDIPVMRRCAFACAPINAHAEVLAIAHHVTTTRGGEGAVRE
                     FCDILMVASGRYVNFLEEYTR"
     gene            complement(2176..3177)
                     /locus_tag="PKX82_10010"
     CDS             complement(2176..3177)
                     /locus_tag="PKX82_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOF52631.1"
                     /translation="MTEPAVHTSQSMAERAIALAQETFDIEAAAIQGLKARTGESFAQ
                     AVALVLKIKGRVVVMGMGKSGHIGRKIAATLASTGTPAMFVHPAEASHGDLGMITASD
                     LVLAISNSGESQEMAAILPVLKRLGAPLIAMTGNGASTMAKHADLWLDTAVSKEACPL
                     NLAPTASTTAQLALGDALAVALLDARGFRAEDFARSHPGGALGRKLLTHVSDVMRSGA
                     AVPKVAPHASFSELMREMSAKGLGAAAVVNSDDRVLGIFTDGDLRRLVEQGADLRSTT
                     AQQVMHGGPSTIGVHALAVDAADLMERKRITSVLVLDEEGRLCGAINSNDLMRAKVI"
BASE COUNT          638 a         1007 c          917 g          665 t
ORIGIN      
        1 ccccgctccg gtggcttcgg cggtggcggt ggcggtggcg gtggcggcgg cggcggtggc
       61 ggccgttccg gtggtggcgg ctacggcggt ggtggcggtg gccgctccgg tggcggcggt
      121 ggtggtggct acggcggtgg ccgcagcagc tactaagcac tcacctgcaa gtacaaaaaa
      181 aggaccctct gggtcctttt ttgttgctgt cattttcctc gatcagggtt cgctgatcct
      241 gcgcttgcga ggtctgccag acagggcttt gtcaaacaat acattaggag ccaagcgcaa
      301 cagtttggcc acccagccca tttgccaggg gatgacccgg taactgtcgc cggcggcaat
      361 cgcggcaaac gccttgtctg caaagtggtg gggctgcatc aggaagggca tggaatagcg
      421 gttcttctgc gtcaggggtg tatcaatgta gccagggcaa atagttacca cccgaatccc
      481 gctggagcgc agctcgccgc gcagactttc acagtaattg atgacggccg ctttgctggc
      541 gcaatacgca ccatggcccg gcaagccccg gattcccgcc acactgccaa tacccaccag
      601 tgccccgctg ccgcgctgca ccatggcgtc gatgaacgga tggaaagtcg cagccagacc
      661 caggttgttg gtagcaaagg tgcgcgccat gacatcaagg tcactgcgta cggcggaatc
      721 catacccaca ctgatgcccg cgttggcgat caccacatcc ggtacaccct ggctcagcaa
      781 acacaactgt ccggcagata cgatactgtc catgtcggac acatctgcgc tgtagatttt
      841 gcagcggtct gcgctgatgt tctgctgcac catccaggtt tgcatttctg cactgcgccg
      901 cgccaccaat gccagtgaaa accccgccag gtaatagcgc caggccaaag cctggccaat
      961 gccactggat gcacctgtaa tgaaaaccag cttggccatg gcctgcccct gtatgtgggt
     1021 tattttgaat cgggcaccag tgtgccgcgc acacgcccct gcaactgcag gatttggtcc
     1081 acattgtcga aatccagact gtctgcggta aaccggtcct gtccgcgcag caattcaact
     1141 ggcttgtggg atttgacttt ctcggtggtc aagaacgcat gcaagaattc accgcggtat
     1201 tccatccggg ggattgcttt ggcgcctgca gcggcgtccg cttcacgcac caccaaagca
     1261 tttccgatca gctgcacctc agacccatcc tcgttggtca atccccgatt ggcagtggct
     1321 gtagtcagtc gtcctttttc atcaaacgag cgaatccgga tagcgtctat ctccagccac
     1381 tgtgtatcag gataatggcg cgctttgtca ccgaccactt ccgagcggat gtgccctttg
     1441 gcatcgtagg tcttgatcgt gaacccctcc atgaagtagt cggggtcatg gctctccaca
     1501 cgcgcaactg ccgccccccc agtctcaggt gttgttcgca ccagccagta cgtcagcaat
     1561 gccaatgtgc ccatgaaaat caggggcagg tacaacagga aacgctccca ggcctcacgc
     1621 aacactggct tcaccgcgtg tactcttcca gaaagttgac gtagcgaccg ctggccacca
     1681 tcagaatgtc acagaattcg cgaactgcgc cttcgccacc gcgcgtggtt gtgacgtggt
     1741 gtgcgatggc cagaacttct gcatgcgcat tgatgggcgc gcatgcaaag gcacagcggc
     1801 gcatcaccgg aatatcgggc cagtcatctc ccatcgccgc tgcagactcc caacccaggc
     1861 cgagttcacg cagtgtctgc tctgcggccg ggcgtttgtc ttcggtaccg aagtgggcat
     1921 gggtaatgcc cagcgcagtg agtcgcgtac gcaagggctt gctgtcgcgc ccggtaatca
     1981 ccaccggggt aatgccgact ttctgcaaca tcttgagacc gtgcccatcc agcgtgttga
     2041 accgcttgag cgtttctccc gtctcggaaa acagcaatcc tccgtccgtc aacactccgt
     2101 ccacgtccag aaaggcgacc tgcacgccct gtgccctgag caggagttcc ggatcaaaat
     2161 tgagagctgg gcgcatcaga ttacctttgc tcgcatgagg tcgttgctgt tgatcgcgcc
     2221 gcagagtcgt ccttcttcat ccagtaccag aacgctggtg atccgcttgc gctccatcag
     2281 atccgcagca tctactgcca gcgcatgcac gccaatggtc gaaggaccgc cgtgcatgac
     2341 ttgctgcgcc gtggtggatc gcaggtccgc accctgctca accaggcgtc gcaggtcgcc
     2401 atccgtaaag atgcccagca cacggtcgtc gctgttcact accgctgctg cccccagccc
     2461 ttttgcactc atctcacgca tcagctcgct aaagctggca tgtggcgcca ccttgggaac
     2521 cgcagcaccg gaccgcatca catcactgac atgtgtcagg agcttgcgcc ccagtgcacc
     2581 tcctggatgc gagcgcgcga agtcctctgc gcggaagccg cgcgcatcca gcagcgccac
     2641 ggccagcgca tcacccagcg ccaactgcgc agtggtgctt gccgtgggcg caagattcag
     2701 gggacaggcc tctttggaca cagccgtgtc aagccacaga tcagcatgct ttgccatggt
     2761 ggaggcgcca ttgcccgtca tggcaatcag tggcgcaccc aatcgcttga gcacaggcag
     2821 gatggcggcc atttcctgcg actctccgct gttggaaatc gccagaacca ggtcggaggc
     2881 agtgatcatc cccagatcgc cgtggcttgc ctctgcagga tgcacaaaca tggcaggtgt
     2941 cccggtagat gccaaagtcg cagcaatctt gcggccaata tggccactct tgcccatgcc
     3001 catgaccacc acgcgcccct tgattttcaa taccagagcc accgcttgcg cgaaggattc
     3061 accagtgcgg gctttgagcc cctggatagc cgcagcttcg atgtcaaaag tctcttgagc
     3121 caacgcgatg gcacgctctg ccatggactg tgaagtgtgg actgcaggtt ctgtcatgcg
     3181 gacattttat agagccagca gtttgggacg gggttattgc taggatg
//
LOCUS       DAOSJM010000289         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_26140_length_3218_cov_4.761935, whole genome shotgun sequence.
ACCESSION   DAOSJM010000289 DAOSJM010000000
VERSION     DAOSJM010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26140_length_3218_cov_4.761935"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1195)
                     /locus_tag="PKX82_10015"
     CDS             complement(41..1195)
                     /locus_tag="PKX82_10015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52632.1"
                     /translation="MTETEPQPLRDTQGYQPRQLVLLGAGHGHVQVLTTLADHPLVGA
                     RITLVAPHPRQLYSGMVPGFVAGHYTLEDCVIPLEPLVRRAGIRWLHRSVKALDADSR
                     NVLLDDGSTLQYDWLSVNTGPVQNRSLIERDLPGAREHGLFVRPIETFGALWPKVAEM
                     GQQKALRIAVIGAGAAGIELAMAVRHRLPNAAVTLLAGPVPVGANYTPAVQARVLAAL
                     KARQITVLQDVALGLNGHEVQLGCGAGLACDVPIIATGAQPAPWLAGSGLAVDAQGFI
                     AVDACQRSTSHPQVFAVGDISTRMDRPLARSGVYAVRAGPALATNLAAAVAGQPLKEH
                     QPPARTLNLIACGDRYAVASWGDFSAQGRWVWWIKDWIDRRFIARFRKEA"
     gene            complement(1200..1877)
                     /locus_tag="PKX82_10020"
     CDS             complement(1200..1877)
                     /locus_tag="PKX82_10020"
                     /inference="COORDINATES: protein motif:HMM:NF022694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HOF52633.1"
                     /translation="MTAGAQALQPLAAGTGDALPAPWRVVGLPGNKVPLAQIDRVELD
                     GHRVLRLRSDKSYGTASHALPPNTPATTLQWKWRVDTAVAQADLRTKQGDDAAVKVCA
                     MFDLPLDKLGFVDRNLMRLARRTSGEYLPAATVCYVWDQGLPAGTVLHNAYTGRVRFM
                     VLNSADTPLGQWATHSRDLAADFVRLFGAEAETVPPLLAIVVGADTDNTHGNSLAYVD
                     ALALVKQ"
     gene            complement(1949..>3218)
                     /locus_tag="PKX82_10025"
     CDS             complement(1949..>3218)
                     /locus_tag="PKX82_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52634.1"
                     /translation="HDSVERYTGLGVEVLQGYARIVNPWTVEIALNDGGNEPQGEPKV
                     LTLTTRSIVIAAGARPTIPPFLATAAAQEVGYVTSDTLWDAFAQLDEVPKRLVVLGGG
                     PIGCELAQSFARLGSQVTQVEMAPRIMAREDAEVSELAAAALRADGVDLLTSHKALRV
                     EKHPAQAVQPEPVEGGPGASTSSARTGGGVLIVEHAGVEKRIAFDQLLCAVGRSARLT
                     GYGLEELGIPTAKTVQTNEYLQTIYPNIYAAGDVAGPYQFTHTAAHQAWYAAVNALFG
                     DFKKFKADYSVIPWATFIDPEVARVGLNEQDAKEQGLAYEVTKYGIDDLDRAIADSEA
                     HGFVKVLTVPGKDRILGVTIVGTHAGDLLAEYVLAMKHGLGLNKILGTIHTYPTLAEA
                     NKYAAGEWKRAHQPHQLLQWVRRFHDWRRS"
BASE COUNT          522 a         1072 c         1056 g          568 t
ORIGIN      
        1 cgccgaccct gcgggcccgc gcatgcgggt gcgctgagcg tcaagcttcc ttgcgaaagc
       61 gggcgataaa gcgccggtcg atccagtcct tgatccacca cacccagcgc ccctgggcgc
      121 tgaagtcgcc ccaggacgcc acggcatagc ggtcaccgca ggcaatcaga ttgagcgttc
      181 tggcgggtgg ctggtgttcc ttgagcggct gccctgccac cgcagcagcc aggttggtgg
      241 ccagtgcagg ccctgcgcgt acggcataca cgccgctgcg ggccagtggt cggtccatgc
      301 gggtgctgat gtcgcccact gcaaacacct gggggtggct ggtggagcgc tggcaggcat
      361 ccactgcgat gaatccctgc gcgtccacgg ccaggccact gcccgccagc cacggcgccg
      421 gttgtgcgcc cgtggcaatg atgggcacgt cgcaggccag acccgcgccg cagcccagtt
      481 gcacttcatg gccattgagc cccaacgcaa catcctgcag caccgtgatc tggcgtgcct
      541 tgagtgccgc cagcacccgc gcttgcacgg cgggcgtgta attggcgcca accggcaccg
      601 ggcccgccag cagggtgacg gctgcgttgg gcaggcggtg gcgcacggcc atcgccagtt
      661 caatacccgc agcgccagcg ccgatgacgg caatgcgcag ggccttctgc tgccccatct
      721 ccgccacctt gggccacagg gcgccaaagg tttcaatggg ccgcacaaac agcccgtgtt
      781 cgcgggcacc gggcaggtcg cgttcgatca gcgagcggtt ctgcaccggc ccggtgttga
      841 ccgagagcca gtcgtactgc agggtgctgc catcgtcgag cagcacgttg cggctgtcgg
      901 catcgagcgc cttgacgctg cggtgcagcc agcggatgcc agcgcggcgc accagcggct
      961 ccagtggaat cacgcagtct tccagggtgt agtgcccggc cacaaagccc ggcaccatgc
     1021 cggagtacag ctggcgcggg tgcggcgcca ccagcgtgat gcgggcgccc accagcgggt
     1081 ggtccgccag ggtggtaagc acctgcacat ggccgtggcc ggcacccagc agcaccagct
     1141 ggcgtggttg gtagccttgg gtgtctctga ggggctgcgg ttcagtttcc gtcatggcgc
     1201 tattgtttca cgagcgcaag cgcgtccaca taggccaggc tattgccatg cgtattgtcg
     1261 gtatcggccc ccaccacgat ggccagcagc ggcggcacgg tctcggcctc cgcaccgaac
     1321 aggcgcacaa agtcggcagc caggtcgcgg ctgtgggtgg cccactgacc cagcggggtg
     1381 tcggcggagt tcagcaccat gaagcgcacc cggcccgtgt aggcgttgtg cagcaccgtg
     1441 ccagcgggca gcccctggtc ccacacataa cagaccgtcg ccgcaggcag gtattcgccg
     1501 ctggttctgc gcgccaggcg catcaggttg cggtcgacaa accccagctt gtccagtgga
     1561 agatcaaaca tggcgcacac cttgaccgcg gcgtcatccc cttgcttggt gcgcaggtca
     1621 gcctgggcca ctgcggtgtc gacacgccac ttccattgca gcgtggtggc cggggtgttg
     1681 ggcggcaagg cgtggctggc agtgccgtac gatttgtcgg accgcaggcg aagcacgcgg
     1741 tggccatcca gctccacgcg gtcgatctgt gccagcggta ccttgttgcc cggcaacccc
     1801 accacacgcc agggtgcagg cagcgcatcg ccggtgcccg ccgccaacgg ctgcagcgcc
     1861 tgtgcacccg ccgtcaccgc caataatgca caaaaagtgc ctatagcccc cgtccatagt
     1921 gcgcgagtag ctatcaatag catagccatc agcttcggcg ccagtcgtgg aaccggcgca
     1981 cccattgcag cagctggtgg ggctggtggg cgcgcttcca ttcgccggca gcatatttat
     2041 tggcctcggc cagcgtgggg taggtgtgga tggtgcccag aattttgttg agccccaggc
     2101 cgtgtttcat ggccagtacg tactcggcca gcaggtcacc tgcgtgggtg ccgacgatgg
     2161 tcacgcccag aatcctgtct ttgcccggga cggtcagcac cttcacaaag ccgtgcgcct
     2221 cgctgtctgc aatcgcgcgg tccaggtcgt cgatgccgta cttcgtgacc tcgtaggcaa
     2281 gaccttgctc cttggcgtcc tgctcgttca ggcccacgcg cgccacctcg gggtcgatga
     2341 aggtggccca ggggatcacc gagtagtcgg ccttgaactt cttgaagtcg ccaaacagcg
     2401 cattcaccgc ggcataccag gcttggtgcg cggccgtgtg ggtgaactgg taggggccgg
     2461 ccacatcgcc cgcggcgtag atgttggggt aaatggtttg caggtattcg ttggtttgca
     2521 cggttttggc ggtggggatg ccgagctctt cgagcccgta accggtcaag cgcgcgctgc
     2581 ggcccacggc gcacagcagc tggtcgaacg caatgcgctt ttccacgcct gcgtgctcaa
     2641 cgatgagcac accccctccc gttcgcgctg agcttgtcga agcgccgggg ccaccttcga
     2701 ctggctcagg ctgcacggcc tgggctgggt gtttttctac tcgcaaggcc ttgtggctgg
     2761 tgagcaggtc cacgccatcg gcacgcagag cggcggcagc cagttcggag acttctgcgt
     2821 cctcccgcgc catgatgcgc ggtgccatct ctacctgcgt gacctgcgag cccagccgcg
     2881 caaagctctg cgccagctcg cagccaatcg ggccaccacc cagcaccacc agacgttttg
     2941 gaacctcatc cagttgggcg aatgcatccc acagcgtatc gctggtgaca taacccacct
     3001 cctgcgcggc agcggtggca agaaaagggg gaattgtggg ccgcgcgcca gcggcgatga
     3061 cgatgctgcg ggtggtcagc gtgagcactt ttggctcgcc ttgcggctcg ttgccgccat
     3121 cgttcagcgc aatctccacc gtccacgggt tgacgatcct ggcgtaaccc tgcagcactt
     3181 ccacaccgag tccggtgtag cgttcgacgc tgtcatgc
//
LOCUS       DAOSJM010000290         3186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_26455_length_3186_cov_4.144363, whole genome shotgun sequence.
ACCESSION   DAOSJM010000290 DAOSJM010000000
VERSION     DAOSJM010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26455_length_3186_cov_4.144363"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PKX82_10030"
     CDS             <1..539
                     /locus_tag="PKX82_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF52635.1"
                     /translation="GWPVPGYDIELRNEDGSPTPDGEPGDLYIHGPSSAMMYWGNRTK
                     TRETFQGGWTKSGDKYVRNADGTYTYSGRSDDMLKVSGIYVSPFEVEATLVQHPAVLE
                     AAVIGVPDAEGLTKTKAFVVLKPGAHATDAELKAFVKDKLAPYKYPRQIEFVTDLPKT
                     ATGKIQRFKLRALESAAE"
     gene            536..1366
                     /locus_tag="PKX82_10035"
     CDS             536..1366
                     /locus_tag="PKX82_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF52636.1"
                     /translation="MTPAVEFATITWRNQPVRLEYQWVGVRDAQAPVMVFLHEGLGSV
                     SMWRNFPDQLCQALGCRGLVYSRPGYGNSTPRAADDHWAPDFMHRQADEVLPAFLRAV
                     GVHVQKQPLWLFGHSDGGSIALLYATHHPKALAGAIVVAPHILVEEVSIASIAQARNA
                     YLETDLRQRLARHHADPDSAFWGWNDIWLDPGFRPWRIDEQLGSITCPLLAVQGVDDA
                     YGTLEQIRGIARKVPQCELLELQACGHSPHKDQPDRLIAAVTHFFQRAVTAAGAVSPP
                     "
     gene            complement(1368..1793)
                     /locus_tag="PKX82_10040"
     CDS             complement(1368..1793)
                     /locus_tag="PKX82_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOF52637.1"
                     /translation="MRIWKSPISVEGLTEVSKNTAVSHLGIEFLEIGDDYLTARVPVD
                     SRTCQPYRLLHGGVAVVLAETLGSCGAACACEPGCKVVGLDINANHLKGTTSGWVTGT
                     ARAVHVGRTTQVWQIDLRNDAGELTCVSRITMAVLQPKA"
     gene            2019..2549
                     /locus_tag="PKX82_10045"
     CDS             2019..2549
                     /locus_tag="PKX82_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOF52638.1"
                     /translation="MRLSTRGRFAINAMIDLALREHAHPVPLAELAVRHHISLSYLEQ
                     VFAKLRQHGLVDSTRGPGGGYTLGMAADAITVADIISAIEDAPGQGAALLEQDLQPGT
                     QDMTRDLWNSLHTTLMAHMKTITLRSLAVEQKAKGFQVEERQTGKKGVFAKRKPEPVR
                     TTAPNSVFALAQAMRG"
     gene            complement(2577..>3186)
                     /locus_tag="PKX82_10050"
     CDS             complement(2577..>3186)
                     /locus_tag="PKX82_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOF52639.1"
                     /translation="VGMAVDIVRAIFGQANIPVKLVPLPYARCMLETKAGKLGGCFDT
                     LPDAQMQRDYLFHAKPLFSDPLLIITRADSKDSAVKIQDLQGKRVLITHGYSYGDEFE
                     SNFLIQRVQSTSDLSNLRMLVAGRAEYSIVYRRILGHQLRGKARDLASQIKPVGQLPN
                     AQLYLSFSRSYPGIPDVIERFNEAHNRLLRDGTIAAITKKWE"
BASE COUNT          612 a          978 c          978 g          618 t
ORIGIN      
        1 cgggctggcc ggtgccaggc tacgacattg aactgcgcaa cgaagatggc agccccacgc
       61 ccgatggtga accgggagac ctgtacatcc acggcccttc cagcgccatg atgtactggg
      121 ggaaccgcac caaaacccgc gagacgtttc agggtggctg gaccaagagt ggtgacaaat
      181 acgtgcgcaa tgccgatggc acctacacct actcggggcg cagcgatgac atgctcaagg
      241 tcagcggcat ctatgtgtcg ccgttcgagg tggaagccac gctggtgcag caccctgccg
      301 tgctcgaggc ggcggtgatc ggcgtgcccg acgccgaggg tctgaccaag accaaggcct
      361 ttgtggtgct caagcctggc gcacatgcca ccgatgcgga gttgaaggct tttgtgaagg
      421 acaagctggc gccctacaag tacccgcgcc agattgaatt tgtcaccgac ctgcccaaga
      481 cggcgaccgg gaaaatccag cgcttcaagt tgcgcgcact ggagtctgcg gctgaatgac
      541 gccggcggtg gaattcgcca ccatcacctg gcgtaaccaa ccggtacgtc tggaatacca
      601 atgggtgggc gtgcgggatg cgcaggcgcc tgtcatggtg tttctccatg aaggcctggg
      661 ttcggtgtcg atgtggcgca acttccccga ccagctctgc caggccctcg gttgccgtgg
      721 gctggtgtat tcgcgtcctg gctacggcaa ctccacgcca cgtgccgccg atgaccactg
      781 ggcgcctgac ttcatgcacc gccaggccga tgaggtgctg cctgcatttt tgagggctgt
      841 cggggtgcac gtgcagaagc aacccctctg gctctttggg cacagcgatg gcggatcgat
      901 tgcgctgctg tatgccacgc accaccccaa ggcgctggcg ggcgctattg tggtggcgcc
      961 ccacattctg gtggaagagg tgtcgattgc cagcattgcc caggccagaa acgcctatct
     1021 ggaaacagac ctgcgccagc gcctggcccg gcaccatgcc gatccggact cggccttctg
     1081 gggctggaac gacatctggc tggacccggg gtttcgcccc tggcgcatcg atgaacagct
     1141 cggaagcatc acatgcccgc tactggcggt gcagggggtg gacgacgcgt atggaaccct
     1201 ggagcagatt cgcggcattg cccgcaaggt gccccagtgt gaactgctgg agctccaggc
     1261 ctgtggacac tccccgcaca aagatcagcc agacaggctg attgcggcag tcacccattt
     1321 ctttcaacgg gcagtcaccg ctgcaggagc ggtatcgcca ccctgattca ggctttgggc
     1381 tgcagcaccg ccatggtgat acgcgagacg caggtcagct cgccagcatc attgcgcaga
     1441 tcgatttgcc acacctgcgt ggtgcgcccc acgtgcaccg cccgtgccgt accggtcacc
     1501 cagccactgg tggtgccttt gaggtggttg gcgttgatgt ccagaccgac gactttgcag
     1561 ccgggttcgc aggcacaggc cgcgccgcag gagcccaggg tttcggccag caccacggcg
     1621 acgcctccgt gcagcagccg gtagggctgg caggtgcgcg agtccaccgg cactcgggcg
     1681 gtcaggtagt cgtctccaat ttccaggaac tcgatgccca gatggctgac ggcggtgttt
     1741 ttgctgactt cggtgaggcc ttcgaccgag atggggcttt tccagatacg cacggtaatt
     1801 ccttgtgaaa caacccacac tataagcaaa ttgtgcttcc agcccctgtc aaaccggcgt
     1861 catcagctat caagttagta gcaattctcg ttcccatcac acccggcaac ctcgcaatac
     1921 ttctgttttt acgaattgaa tgtgcactaa atgctggttt actatgtcct gtcaaagtcc
     1981 tacagtggct tcctcgctat tcgctttaag gaaacaccat gcgactcagt acccgcggcc
     2041 gatttgccat taacgcaatg attgaccttg cgctgcgcga gcacgcgcat ccggtgccgc
     2101 tggccgaatt ggccgtgcgc caccacatct ccctgtccta tctggagcag gtatttgcca
     2161 agctgcgcca gcacggtctg gtggacagca cgcgcggacc cggcggtggc tataccctgg
     2221 ggatggccgc cgacgccatc accgtggccg acatcatttc cgccatcgag gatgcaccgg
     2281 gccagggagc ggccctgctg gagcaggatc ttcagcccgg cacgcaggac atgacgcgcg
     2341 acctgtggaa cagcctgcac accaccctga tggcgcacat gaaaaccatc accctgcgca
     2401 gcctggcggt ggagcagaag gccaagggct ttcaggtcga agagcgccag accggcaaga
     2461 aaggtgtgtt tgccaagcgc aagccagagc cggtgcgaac aacggctccc aattcggtgt
     2521 ttgcgctggc gcaggctatg cgcggctgaa tccgctgggg cgcggtgtct gcgcccctat
     2581 tcccattttt tggtaatggc cgcgatggtt ccgtcgcgca acaggcggtt gtgcgcttcg
     2641 ttgaaacgct ctatgacgtc cggtataccc gggtagctgc gggagaagga aaggtagagc
     2701 tgggcgttgg gcaactgccc caccggcttg atctggctgg ctaaatccct ggctttcccg
     2761 cgaagctggt ggcccagaat ccggcgatag acgatggaat actctgcacg accagccacg
     2821 agcatgcgca gattgctgag gtccgatgtg ctttgcacac gctggatcag gaaattgctt
     2881 tcgaactcgt cgccatagct gtagccatgg gtgatcagca cccgcttgcc ttgcaggtcc
     2941 tgaatcttca cagcgctgtc tttggaatcc gcccgcgtga tgatcagcag cgggtctgag
     3001 aacagcggtt tggcgtggaa cagatagtca cgctgcattt gcgcatcggg cagcgtgtcg
     3061 aagcagcccc ccagcttgcc cgccttggtt tccagcatgc accgtgcata gggcagcgga
     3121 accagcttta caggaatatt ggcctgcccg aagatggcac ggacgatgtc caccgccatg
     3181 cccacc
//
LOCUS       DAOSJM010000291         3176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_26548_length_3176_cov_5.740147, whole genome shotgun sequence.
ACCESSION   DAOSJM010000291 DAOSJM010000000
VERSION     DAOSJM010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26548_length_3176_cov_5.740147"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PKX82_10055"
     CDS             <1..702
                     /locus_tag="PKX82_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HOF52640.1"
                     /translation="AHHMFVGESGISRVISRTCAAMNELKTDDVTKLRAAGVIDLPTL
                     QRYLNFHFSVTIDLFGADESSNAATFYTTGLKGRFEEGKRNDDHMLKGQTYKVLAVQG
                     NQLVEREVPMLNALNEVLRDDYIKDSIGGVERWNKVIEKAGIPFRLKTPHKAFHRNIG
                     TLAGVKVAPDGRVVSEAEWKANVDNWLPSGADRAFVASLMGRVVEPGKFANWIAPPVM
                     GINRQPVDFEYVRFN"
     gene            1029..2315
                     /gene="boxA"
                     /locus_tag="PKX82_10060"
     CDS             1029..2315
                     /gene="boxA"
                     /locus_tag="PKX82_10060"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HOF52641.1"
                     /translation="MTMPEAVIKQHLIDPEICIRCNTCEAICPVGAITHDSRNYVVKA
                     EVCNLCMACISPCPTGSIDNWRTMPLVRAYSIEDQFSWDELPAELSAEQLAEQGISEA
                     QVAQTEAAPAASADPTGETAFNSAAFGATVPPWSAAHAYTNLYGPKAAEKCVTATVVG
                     NVRVTEVGATQGSDYDTHHIMLDFGAMPFPVLEGQSIGIIPPGQDDSGRTHHARQYSI
                     ASPRNGERPGYNNLSLTVKRVLEDHQGKPVRGVGSNYMCDLKIGDKVQVIGPFGSSFL
                     MPNHPRSNIVMICTGTGSAPMRAMTEWRRRLRASGKFEGGKLMLFFGARTQQELPYFG
                     PLQNLPKDFIDINFAFSRTPNQPKRYVQDLMRERAADLAPLLNDANTYFYVCGLKSME
                     EGVVMALRDIATKANLSWDTVGAALKREGRLHLETY"
     gene            2315..3115
                     /locus_tag="PKX82_10065"
     CDS             2315..3115
                     /locus_tag="PKX82_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOF52642.1"
                     /translation="MSDYQTLKITQRSAGVAQVTMARPAVFNAFDEAMIGELDTVFAA
                     LVADDTVRVVVLAGEGKHFSAGADLQWMQRASTASREWNLEDARRFAGMLSRIETCPK
                     PTVARVQGAALGGGVGLACACDIAIAADNASFSVSEAKFGILPAVIGPYVTNAVGKRQ
                     AKRLALTTERIHAADALAMGLVQQAVTLDALDAAVDATVTQLLAGGPNAQREIKALFS
                     QLEVGAITPEVRELTAQTISRVRGTPEAREGFTAFLEKRPATWIPKTP"
BASE COUNT          658 a         1079 c          912 g          527 t
ORIGIN      
        1 gcgcaccaca tgttcgtggg cgagtccggc atcagccgcg tgatcagccg cacctgcgct
       61 gcgatgaacg agctcaagac cgacgatgtg accaagctgc gcgccgccgg cgtgatcgac
      121 ctgcccacgc tgcagcgcta cctgaacttc cacttcagcg tcaccatcga cctgtttggc
      181 gccgacgagt ccagcaacgc cgccaccttc tacaccaccg gcctcaaagg ccgtttcgaa
      241 gagggcaagc gcaacgacga ccacatgctc aagggccaga cctacaaggt actggccgtg
      301 cagggcaacc agctggtcga gcgcgaagtg ccgatgctca acgccctgaa cgaagtgctg
      361 cgcgacgact acatcaagga ctccatcggt ggcgtcgagc gctggaacaa ggtcatagag
      421 aaggccggca ttccgttccg cctgaaaacc ccgcacaagg ccttccaccg caacatcggc
      481 accctggccg gcgtgaaggt ggccccggat ggccgcgtgg tgtccgaagc cgaatggaaa
      541 gccaatgtgg acaactggct gccctccggc gccgaccgcg cttttgtagc cagcctgatg
      601 ggccgcgtag tggaacccgg caagtttgcc aactggattg cgcccccggt gatgggcatc
      661 aaccgccagc cagtggattt tgagtacgtc cggttcaatt gatcttgcgt aacgctgcgg
      721 cttttgctga gctcgcagcg gggcaggagt atgtacctcc gctcatgtct cccgcagagg
      781 gcgttgccct ctgctcctcc tttacttcgc tgcggcacac acccctgccc cgctgctgcg
      841 aacggtattg cttgcagaca actccacacg ctacgtaaga tctggtgggt tgggtgttct
      901 gcaaagtgag gtaaaggagg cggccgaagg ccgggggaca cgaacggcgc agaacaccca
      961 agccgccaga tcctgagcca atgcaggcta acctttgaac acaacgcagg ctgcaaaaac
     1021 agagagacat gaccatgcca gaagccgtca tcaagcaaca cctgatcgac cccgaaatct
     1081 gcattcgctg caacacctgc gaagctatct gcccagtcgg cgccatcacg catgactcgc
     1141 gtaactacgt ggtcaaggcc gaggtgtgca acctgtgcat ggcctgcatt tcaccctgcc
     1201 ccaccggctc catcgacaac tggcgcacca tgccgctggt acgcgcctac tcgatcgaag
     1261 accagttcag ctgggacgaa ctgccagccg aactcagcgc agaacagctg gccgaacaag
     1321 gcatctccga agcccaggtt gcccagacgg aagcagctcc agccgccagc gccgacccca
     1381 cgggcgagac ggcattcaac tcggcagcct ttggcgccac cgtgccgccc tggtcggcgg
     1441 cccatgccta caccaacctc tatggcccca aggctgcaga gaagtgtgtc actgccacgg
     1501 tggtgggcaa tgtgcgcgtc accgaagtcg gcgccacgca aggcagcgac tacgacacgc
     1561 accacatcat gctggacttt ggcgccatgc ccttcccggt ccttgaaggc cagtccatcg
     1621 gcatcatccc gcctggacag gatgactcgg gtcgtacgca ccatgcacgc cagtactcga
     1681 ttgccagccc ccgcaacggc gagcgccccg gctacaacaa cctgtcgctg accgtcaaac
     1741 gcgtgctgga agaccaccag ggcaagccgg tgcgcggtgt ggggtccaac tacatgtgcg
     1801 acctgaagat cggcgacaag gtgcaggtga tcggcccgtt tggcagcagc ttcctgatgc
     1861 ccaaccaccc gcgttccaac atcgtgatga tctgcaccgg caccggcagc gcccccatgc
     1921 gcgccatgac cgaatggcgc cgccgcctgc gcgcctcggg caagttcgaa ggcggcaagc
     1981 tcatgctgtt tttcggcgcc cgcacccagc aggaactgcc ctacttcggc ccgctgcaaa
     2041 acctgcccaa agatttcatc gacatcaact tcgcgttctc acgcaccccc aaccagccca
     2101 agcgctatgt gcaggacctg atgcgcgaac gcgccgccga tctggcgccg ttgctgaatg
     2161 acgccaacac ctacttttat gtgtgtggtt tgaagagcat ggaagaaggc gtggtcatgg
     2221 cgctgcgcga cattgccacc aaggccaacc tgtcgtggga caccgtaggc gcagccctca
     2281 aacgcgaagg caggctccat ttagagactt attgatgagc gactatcaga ccctcaaaat
     2341 cacacagcgc agcgccggcg tcgctcaggt caccatggcc cgccccgcgg tgttcaatgc
     2401 gtttgacgaa gccatgattg gcgaactgga tacggtcttt gctgctctgg tggccgacga
     2461 cacggtgcgc gtggtcgtgc tggcaggcga aggcaagcac ttcagcgccg gcgccgatct
     2521 gcaatggatg caacgtgcca gcaccgcttc gcgcgaatgg aatctggaag acgcacgccg
     2581 ctttgccggc atgctctcgc gcatcgaaac ctgccccaaa cccaccgtcg cccgcgtgca
     2641 aggtgcggcg ctgggcggtg gcgtaggtct ggcctgcgcc tgcgacattg ccattgctgc
     2701 agacaacgcc agcttctccg tgagcgaagc caagtttggc atcctgcccg ccgtgattgg
     2761 cccctatgtg accaacgccg tgggcaagcg ccaggccaaa cgcctggcac tcaccaccga
     2821 acgcatccat gcggcggatg cactggcaat gggtctggtg cagcaggcgg tgacgctcga
     2881 cgcactggac gcggcggtcg acgccaccgt cacccaactg ctggccgggg gccccaatgc
     2941 ccagcgcgaa atcaaggcgc ttttttccca gctggaagtc ggcgccatca cccccgaagt
     3001 acgcgaactc accgcacaaa ccatcagccg ggtgcgcggc acgccggaag cccgcgaagg
     3061 ctttaccgca ttcctggaaa aacgtcctgc aacctggatt cctaaaacac catgacccaa
     3121 gcattcatct gtgacgccat ccgcaccccc tttggccgct acggcggcgc cctgtc
//
LOCUS       DAOSJM010000292         3161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_26727_length_3161_cov_4.613007, whole genome shotgun sequence.
ACCESSION   DAOSJM010000292 DAOSJM010000000
VERSION     DAOSJM010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26727_length_3161_cov_4.613007"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PKX82_10070"
     CDS             complement(<1..190)
                     /locus_tag="PKX82_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52643.1"
                     /translation="MSKSKHAKVLILGSGPAGYTAAVYAARANLNPVLVTGMAQGGQL
                     MTTTEVDNWPADVHGVQGP"
     gene            315..989
                     /locus_tag="PKX82_10075"
     CDS             315..989
                     /locus_tag="PKX82_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF52644.1"
                     /translation="MALLSNLELIRRVPLFAQLSTSQAEALVGAVTKQRFKRGECIVT
                     QGQTSNALFILLTGRARVLMSDKRDREVILASMQAGDHVGEMSLIDGEPHSATVQAEI
                     MTDTLVLGRKDFLRCLSENSAMAHSVMRELVHRLRVADHKIGSLALMGVYARVANVML
                     ESAVADTGGGLLIPHKVSRQDVAKMVGASREMVSRVMKDFEEQGFLQTLDQGLIRVHD
                     RRQAPR"
     gene            1029..>3161
                     /locus_tag="PKX82_10080"
     CDS             1029..>3161
                     /locus_tag="PKX82_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOF52645.1"
                     /translation="MTYSLHTLQSSSSSAPTEPTGIGRFANEIALVAGFLLLALWVIA
                     LTTYSLGDPAWSTSGTGMAVANRVGRFGAWIADASYFLMGFSVWWCVAIGVRLWLSLL
                     ANRLRGDVLHPVVSVPGQDVSRAQLLRRMAFWCGLAMLLYGSTTLEWSRLYRLEAALP
                     GHSGGALGYLLGSVSVKWLGFAGSGLVAIVLGVVGAAMAFNFSWMHWAERIGSRVDAL
                     LESQREKRELAQDLQMGQQAMKEREDVVFEEREEIEVHHPQPVVIVEPVLAAPPRSER
                     VAKESQKPLFSEMPDSKLPQVALLDGAQLRQETVAPETLEMTSRMIEKRLKDFGVEVR
                     VVLAQPGPVITRYEIEPATGVKGSQIVGLAKDLARSLSLVSIRVVETIPGKNYMALEL
                     PNAKRQSIRLSEILGSQVYNEAKSMLTMGLGKDIIGNPIVADLAKMPHVLVAGTTGSG
                     KSVGINAMILSLLYKAEARDVRLLMIDPKMLEMSVYEGIPHLLCPVVTDMKQAANGLN
                     WCVAEMEKRYKLMSKMGVRNLAGFNAKIDEAKARGEFIYNPFSLTPESPEPIDRLPHI
                     VVVIDELADLMMVVGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPT
                     RIAFQVSSKIDSRTILDQMGAEALLGMGDMLYMPSGTGLPIRVHGAFVSDEEVHRVVS
                     YLKSQGEPNYIDGVLEGGTVDGDGDAALD"
BASE COUNT          590 a          864 c         1030 g          677 t
ORIGIN      
        1 ccgggccctg cacgccgtgc acatctgccg gccagttgtc cacttcggtg gtagtcatga
       61 gctggccacc ctgtgccatg ccggtcacca gcaccggatt gaggttggcg cgtgcggcat
      121 agacagcggc ggtgtagcca gcggggccag agcccagaat gagcaccttg gcgtgtttgg
      181 atttggacat gggtaacctt tgtggcaaaa atgggcttgg gtccgtgtac atttcgggtc
      241 aacgcggacc atttcaatgc aggctctttc ctgattgtaa gaaaccgcac actgtctgat
      301 cggagagctg cgcaatggca ttgctgagta acctggaatt gattcgaaga gttccgctgt
      361 ttgcacagct gagcacctcg caggcagaag cactggtggg cgctgttacc aagcagcgtt
      421 tcaagcgcgg cgaatgcatt gtcacgcagg ggcagacttc caacgcgtta ttcattctgc
      481 ttaccggccg tgcccgcgtc ctgatgtcgg acaagcgcga ccgcgaagtg attcttgctt
      541 ccatgcaggc cggcgaccat gtgggcgaga tgagtctgat tgatggtgag ccccattcgg
      601 ctacggtgca ggccgaaatc atgactgaca ccctggtgct cgggcgcaag gattttttgc
      661 gttgcctgtc tgaaaattcc gccatggcgc actcggtcat gcgtgaattg gtacaccgtt
      721 tgcgtgttgc cgaccacaaa atcggttcac tggcgttaat gggcgtatat gcccgtgtgg
      781 ccaatgtcat gctggagtct gccgttgcag ataccggtgg cggattgctg atcccgcaca
      841 aggtttcccg acaggatgtt gccaagatgg tcggcgcatc gcgggagatg gtcagccgtg
      901 tcatgaaaga ttttgaggag caggggttcc tgcaaaccct ggaccagggt ttgattcggg
      961 tgcatgaccg ccggcaggcg ccgcgctgag tcgctgctcg ggtatgcttg cccgtactgt
     1021 ttctgtccat gacctactct ctccatactc tccaatcctc ttcttcgtcg gcgccaactg
     1081 aacccacagg cattggccga ttcgccaacg aaattgcgct ggttgccggt tttttattgc
     1141 ttgccttatg ggtgattgcg ctcaccacct attccctggg tgatccagcc tggtcgactt
     1201 cggggacggg catggccgtg gccaaccggg tggggcgctt tggcgcctgg attgccgatg
     1261 ccagctactt tctgatgggt ttctccgtgt ggtggtgcgt ggccattggt gtacgcctgt
     1321 ggttgtcact gctggctaac cggcttcggg gggatgtact gcatccggtt gtctccgttc
     1381 ccgggcagga tgtgtcgcgc gcccagttac tgcggcgcat ggctttctgg tgcggtctgg
     1441 ccatgttgct gtacggcagc acgacactgg aatggtcccg tttgtaccgg ctggaagccg
     1501 ctctgccggg acacagtggt ggtgccttgg gttatctgct gggctcggtc agcgtcaagt
     1561 ggctgggttt tgccggttcc ggcctggtgg ccattgtgct gggcgtcgta ggcgctgcca
     1621 tggcgttcaa tttctcgtgg atgcattggg ccgagcgcat tggttcccgg gtcgatgcgt
     1681 tgctggagtc gcagcgtgag aagcgtgagt tggcgcagga cctgcaaatg ggccagcagg
     1741 ccatgaagga gcgcgaagac gtggtcttcg aggagcgcga ggaaatcgag gtccaccatc
     1801 cgcagcccgt ggtgattgtg gagcccgtgt tggctgcacc gccccgcagc gaacgggtgg
     1861 ccaaagaaag ccagaagccg ctgttctcgg aaatgccgga cagcaaactg ccccaggtgg
     1921 cgttgctcga tggtgcgcag ttgcgacagg agaccgtagc acccgaaacg ctggagatga
     1981 ccagccgcat gatcgagaag cgcctgaagg actttggcgt ggaagtgcgt gtagtcctgg
     2041 cgcagccagg tccggtgatt acccgctacg agattgagcc cgccacaggt gtgaagggtt
     2101 cgcagatcgt gggtctggcc aaggacctgg cacgatcctt gagcctggtg tcgatccgcg
     2161 tggtggaaac catccccggc aagaattaca tggcgctgga attgcccaat gccaagcgcc
     2221 agtcgatccg gctgtccgag atcctgggct cgcaggtgta caacgaagcc aagtccatgc
     2281 tgaccatggg gctgggcaaa gacatcattg gcaaccccat cgtggccgat ctggccaaga
     2341 tgccgcacgt gctggtggcg ggaaccactg gttcgggcaa gtcggtgggg atcaatgcca
     2401 tgatcctgtc gctgctctac aaggccgaag cccgcgatgt gcggctgctg atgatcgacc
     2461 ccaagatgct ggaaatgtcg gtgtacgaag gtattccgca cctgctgtgc ccggtggtga
     2521 ccgacatgaa gcaggccgcc aacggcctga actggtgcgt ggccgagatg gagaagcgct
     2581 acaagctcat gagcaagatg ggggtgcgca acctggcggg tttcaatgcc aagatcgacg
     2641 aagccaaggc acgtggagag ttcatttaca acccgttcag cctgacacca gaaagccccg
     2701 agccgatcga ccgcttgccg cacatcgtcg tggtgattga tgaactggcc gacctgatga
     2761 tggtggtcgg caagaagatt gaagaattga ttgcccgtct ggcacaaaaa gcccgcgccg
     2821 ccggcatcca cctgattctg gcgacccagc gccccagtgt ggacgtgatc accggcctga
     2881 tcaaggccaa cattcccacc cgcattgcgt ttcaggtgag cagcaagatt gacagccgca
     2941 cgattctgga ccagatgggc gccgaggccc tgctgggtat gggcgacatg ctctacatgc
     3001 ccagcggtac cggtttgccg attcgtgtgc acggcgcgtt tgtgagtgac gaggaggtgc
     3061 accgcgtggt gagctacctc aagagccagg gcgaacccaa ctacattgac ggcgtgctgg
     3121 aaggtggtac ggtcgatgga gacggcgatg ccgcgctgga c
//
LOCUS       DAOSJM010000293         3139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_26956_length_3139_cov_3.515240, whole genome shotgun sequence.
ACCESSION   DAOSJM010000293 DAOSJM010000000
VERSION     DAOSJM010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26956_length_3139_cov_3.515240"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..540
                     /locus_tag="PKX82_10085"
     CDS             4..540
                     /locus_tag="PKX82_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOF52646.1"
                     /translation="MNTIATEQLTYLRGYPQEALDKVAQMLRDNRVGTWLTNRYPQAH
                     GIRTDRALYDHVQELKSQYLRGAEPLSKVAFDSKLQVVQNALGTHTTVSRVQGSKLKS
                     KREIRIAALFKDAPPEFLRMIVVHELAHLRERAHDKAFYQLCTYMESNYHQLEFEVRV
                     YLTHLEHTGQRLWEGQTD"
     gene            560..1159
                     /locus_tag="PKX82_10090"
     CDS             560..1159
                     /locus_tag="PKX82_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52647.1"
                     /translation="MHALRSPASLDWHGLGRRRFLRLLTTIGATAVAGSLVTACGNAT
                     SGPLRLLVLSPSQATTLAALAQAVVPSQPGFPNVAQAQVVQRLDEELWLSDSSIQDDL
                     RAALGALEWMPLAYGHFTRFSRLATFEQQALIQRWMESRFDLVRAIATNLKLVAHFMY
                     FGHPSTWGAIGYDGPFQKLQPILSEQRKAYAQRIAGGAQ"
     gene            1156..2715
                     /locus_tag="PKX82_10095"
     CDS             1156..2715
                     /locus_tag="PKX82_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006415024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HOF52648.1"
                     /translation="MTGRIFQGKDLTAGQTVEADVCIIGSGCGGATLAKQLSAAGRSV
                     VILERGGYYTEADFDQDELNMNGKLSSNRSLKSSTDGGTLLLSGNNVGGASVHYWADT
                     YRTPDDRLAHWAQRYGLTGHGLAEMAPAWTELDGVLNVHRADEDHHNRMNQLLRAGAQ
                     KLGWAGSAVPQARRGCVKSGHCMQGCLYAAKQSQLVTHIPQAVALGARLYADVQARTL
                     QFQTGQQDKVQSLLCDVMDRATNRPSGKQLTVQAQQFVVAAGGYYSAPFLIANGLQSR
                     LPALGQHFGMNPTAWAFGLYDEDIVLWRNIPAAFGVEEFPLARFDAAKKYTEGGYLLM
                     ANQVQPGSLAGTMPLLGDEIGEWMGQAKRIGSTIIWLDDYEEELGTIGVGSDGSPKVE
                     YPYGPVTQAMLRDSLKKQVTMLFATGAKKVLLGSAQRILLDKPGDLSAIDSIAITAGG
                     LFMAAPHPFGGCRMGTDPKTSVVGMDHRMHGFSNLFVADPSVFPTGPSVDPSFTIMAF
                     SYVAAKHMLAA"
     gene            complement(2725..>3139)
                     /locus_tag="PKX82_10100"
     CDS             complement(2725..>3139)
                     /locus_tag="PKX82_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52649.1"
                     /translation="PDQEVCMAATGTSAPCLSLFEPLRVAEPTSGSQVIAPGSRVGDS
                     AWAKFEPVHHRALFDAAFRQPLHAERDALEAQLFVAASQDAPDWTAIAQQADAWRTRW
                     AQQASASEFRAPGRLGAWWRKRALRELAEGNACFP"
BASE COUNT          602 a         1038 c          966 g          533 t
ORIGIN      
        1 gctatgaata ccatagcaac tgagcaacta acctatctgc ggggctatcc gcaagaggcc
       61 ctggacaagg tcgcgcagat gctgcgcgat aaccgggtgg gcacctggtt gaccaaccgg
      121 tatccgcaag cccatggcat acgtaccgac cgcgccctgt acgaccacgt acaggaactc
      181 aagagccagt acctgcgcgg agcggagccc ctgagcaagg tggcttttga cagcaagctg
      241 caggtggtgc agaacgccct gggcacccac accacggtgt cgcgtgtgca gggctccaag
      301 ctcaagtcca agcgcgaaat ccgcattgct gcgctgttca aggacgcacc gcccgagttt
      361 ctgcggatga tcgtggtgca tgagctggcc cacctgcgcg agcgcgcgca cgacaaggcc
      421 ttctaccagc tgtgtaccta catggagtcg aactaccacc agctcgaatt tgaagtgcgg
      481 gtctacctca cgcacctgga gcatactggc cagcggctct gggaaggcca gaccgactga
      541 aacttgaagg acgacaacca tgcatgcttt gcgttccccg gcttctctgg actggcacgg
      601 cctgggtcgc cgccgtttcc tgcggctgct gaccaccata ggggcaaccg cagttgcagg
      661 ctcactggtt actgcctgcg gcaatgcaac cagcgggcct ctgcggctgc tggtgctttc
      721 tccgtcgcag gccaccacct tggctgccct ggcccaggcc gtggtaccca gccagcccgg
      781 ttttcccaac gtggcccaag cccaggtggt gcagcgcctg gacgaagagt tgtggctgtc
      841 cgactcctcg atccaggacg acttgcgcgc tgccctgggt gcactggaat ggatgccgct
      901 ggcgtatgga cacttcacac gcttctcccg attggccacc ttcgagcaac aggcgctgat
      961 ccagcgctgg atggagagcc ggtttgacct cgtgcgggcg atcgcgacca acctgaagct
     1021 ggtggcgcac ttcatgtatt tcggacaccc cagcacctgg ggtgccatcg gatacgacgg
     1081 gccgttccag aagctgcaac ccatcctgag tgagcagcgc aaagcctacg cacagcgcat
     1141 tgccggaggt gcccaatgac cggccgcatc ttccagggca aggacctgac tgcaggccag
     1201 accgttgagg ccgatgtttg catcatcggc tcgggctgcg gtggcgccac gctggccaaa
     1261 caactctctg ctgccgggcg cagcgtcgtc attctggagc ggggtggcta ctacaccgaa
     1321 gccgacttcg accaggacga gctcaacatg aacggcaagc tctccagcaa ccgcagcctc
     1381 aagtcgtcta ccgatggtgg cacgctgctg ctgtcgggca acaacgtggg cggtgcctcg
     1441 gtgcactact gggctgacac ctaccgcacc cccgatgacc ggctggcgca ctgggcgcag
     1501 cgctacgggc tgacgggcca cggtctggcc gaaatggctc ctgcctggac tgagctcgac
     1561 ggtgtgctca acgtgcaccg cgccgatgaa gatcaccaca accgcatgaa ccagctgctg
     1621 cgtgccggtg cgcaaaaact gggctgggct ggcagcgcgg taccgcaggc acgccgtggc
     1681 tgcgtcaagt ccgggcactg catgcagggc tgcctctatg ccgccaaaca gagccagctg
     1741 gtcacccaca ttccgcaggc cgttgcactc ggtgcacgcc tgtacgccga cgtgcaggcg
     1801 cgcaccctgc agtttcagac cggacagcag gataaggtgc aaagcctgct gtgcgatgtg
     1861 atggaccgtg ccaccaaccg cccgtccggc aaacagctga ccgtgcaggc ccaacagttt
     1921 gtggtcgccg cgggcggcta ttacagcgcg ccattcctga tcgcgaatgg gctgcaatcc
     1981 cggctgcccg cgctgggcca gcactttggc atgaacccca cggcgtgggc ctttggcctg
     2041 tacgacgaag acatcgtcct gtggcgcaac atacccgccg catttggcgt ggaagaattt
     2101 cccctggccc gctttgatgc cgcaaaaaaa tacaccgaag gcggttacct gctgatggcc
     2161 aaccaggtgc aacccggcag cctggccggc accatgccgc tgctcgggga cgaaatcggc
     2221 gaatggatgg ggcaggccaa acgcattggc agcaccatca tctggctcga tgactacgag
     2281 gaagaactcg gcaccatcgg ggtgggcagc gacggttcac ccaaagtgga gtacccgtac
     2341 ggcccggtga cgcaggcgat gctgcgggat tcactcaaga agcaggtcac catgctgttt
     2401 gccaccggtg ccaaaaaggt gctgctgggc agcgcccaac gcatcctgct ggacaagccg
     2461 ggcgaccttt cggcaattga cagcatcgcc atcaccgcgg gcggcctgtt catggcggca
     2521 ccccatccgt ttggcggttg ccgcatggga accgacccca agacgtcggt cgtcggcatg
     2581 gaccaccgga tgcatggttt ctcgaacctg tttgtagccg acccttcggt atttcccacg
     2641 gggccttcgg tggaccccag cttcaccatc atggccttca gctacgtggc ggccaaacac
     2701 atgctggcgg catagcgaag ctactcacgg gaaacaggcg ttcccttcgg ccagctcgcg
     2761 cagagcccgc ttgcgccacc aggcgcccag acggccaggg gcccgaaatt cggaagcgct
     2821 ggcctgctgt gcccaccgcg tgcgccaggc atcggcttgc tgtgcgatgg ccgtccagtc
     2881 gggtgcatcc tggctcgcag ccacaaacag ctgcgcttcc agcgcatcac gttcggcgtg
     2941 caagggctgg cgaaatgccg catcaaacaa cgcacggtgg tgcaccggct cgaacttcgc
     3001 ccaggcgctg tcccccaccc tgctacccgg cgcaatcacc tgcgagccgg acgtgggctc
     3061 cgccacacgc agaggctcaa acagcgacag gcagggtgcc gaagtgcctg tggcggccat
     3121 gcacacctcc tggtcgggc
//
LOCUS       DAOSJM010000294         3129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27048_length_3129_cov_3.753416, whole genome shotgun sequence.
ACCESSION   DAOSJM010000294 DAOSJM010000000
VERSION     DAOSJM010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27048_length_3129_cov_3.753416"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1232)
                     /locus_tag="PKX82_10105"
     CDS             complement(150..1232)
                     /locus_tag="PKX82_10105"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOF52650.1"
                     /translation="MSLVPYGLARRVLFAMDPEVAHDLTMAGLAATQGNPLKMAYGAG
                     RVDDPIELAGLRFPNRVGMAAGLDKNARCIDGLGAMGFGFVEVGTVTPKGQPGNPKPR
                     MFRLPEANALINRLGFNNDGLDAFVANVQRSALRRDKNPALLLGLNIGKNAATPIENA
                     TADYLTCLDGVYPHADYITVNISSPNTKNLRALQSDEALDALLGAIAERREALVREHG
                     KRKPIFVKIAPDLDEAQVSVIAATLKRYGTNARGQANNDWGVVATNTTLSRDAVKGMA
                     HAEETGGLSGAPVLALSNAVITQLRAALGKSFPIIGVGGVMSGEDAVSKIKAGADVVQ
                     IYTGLIYKGPELVREAALAIRNIKAK"
     gene            1278..1985
                     /gene="rpiA"
                     /locus_tag="PKX82_10110"
     CDS             1278..1985
                     /gene="rpiA"
                     /locus_tag="PKX82_10110"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOF52651.1"
                     /translation="MTNSQTPAPANVLSQDELKTLVGQAALQYVVPGEIVGVGTGSTV
                     NKFIDALATIKDQIKGAVSSSVASTERLQALGISVFDSNAVDRLSVYIDGADEIDGAG
                     NMVKGGGAALTREKIVAAQSAKFVCIADESKLVQTLGKFPLPVEVIPMATQRIIAQFA
                     AKGGTGKVRLKDGKPLVTDNGQFIVDVTGLQITDPLAFEAEVSQWPGVVTVGVFAYQR
                     AQVCLLGTASGVKTLTF"
     gene            complement(2007..2636)
                     /locus_tag="PKX82_10115"
     CDS             complement(2007..2636)
                     /locus_tag="PKX82_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HOF52652.1"
                     /translation="MKRHTLALLLTSAALLGASAVAQAQVVYRIVGPDGKVTFSDKPP
                     SADQGKVAATGVGAAGAASATNLPFELRQVVAKYPVVLYTSSDCAPCGAGRSLLTSRG
                     VPFNERTVTTAEDTAALQRLTGENSIPVLTIGSQRLKGYSDAEWSQYLDAAGYPRTST
                     LPAGFKNAPATPLVAVQKPAERKAEPAPAPVEAAPPVGPSPSNPAGITF"
     gene            complement(2667..>3129)
                     /locus_tag="PKX82_10120"
     CDS             complement(2667..>3129)
                     /locus_tag="PKX82_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOF52653.1"
                     /translation="RALFDKMLAAKNFQSGMQTLRQIEFALFDMLLHTSHDPAVDFLP
                     LLHTVRQEVAVMHPPAWSRTAHTFSHIFAGGYAAGYYSYKWAEVLSADAYAAFEETAA
                     ADGTPSAETGRKYRESILEAGGSRPAMESFKAFRGREPTLDALLRHQGMAA"
BASE COUNT          534 a          915 c         1052 g          628 t
ORIGIN      
        1 cgtagcgagc ccggcgatgt ctcgcgcatc aagcatgaac ttctcaaact cgacggatgc
       61 tctctggaag gacgctggga tgggcatgtc aatctatatt tgctacaaaa atagtagctg
      121 ttcgcgcaat accaacgggg gctagaggcc tatttggctt tgatattacg aatcgccaga
      181 gccgcctcgc gcaccagctc cggtcccttg tagatcaggc cggtgtagat ctgcaccacg
      241 tcggcgcccg ccttgatctt gctcaccgcg tcttcaccgc tcatgacgcc gcccacccca
      301 atgatgggga agctcttgcc cagtgccgcg cgcagctggg tgatgacggc attgctcagc
      361 gccagtaccg gcgcaccaga gaggccaccg gtttcctcgg cgtgggccat gcctttgacc
      421 gcatcgcggc tcagtgtggt gttggtggcc accacgcccc agtcgttgtt ggcctggccg
      481 cgcgcgttgg tgccgtagcg tttgagcgtg gcggcgatca cggacacctg ggcttcatcc
      541 aggtcgggcg cgattttcac aaagatgggt ttgcgcttgc cgtgctcgcg caccagcgcc
      601 tcgcggcgct cggcaatggc acctaagagt gcatccagcg cctcgtcgct ctgcagcgcg
      661 cgcaggttct tggtgttggg gctggagatg ttgaccgtga tgtagtccgc gtgcgggtag
      721 acgccgtcca ggcaggtcag gtaatcggcc gtcgcatttt ctatcggcgt ggccgcgttc
      781 ttgccgatgt tcaggcccag tagcagggcc gggttcttgt cgcggcgcag ggctgagcgc
      841 tgcacattgg ccacaaaggc gtcgagcccg tcgttgttga agcccaggcg gttgatgagt
      901 gcgttggctt ctggcaggcg gaacatgcgc ggtttggggt tgccaggctg ccctttgggg
      961 gtgacagtgc ccacttcgac aaagccaaat cccatggcgc ccaggccgtc gatgcagcgg
     1021 gcgttcttgt ccaggcccgc cgccatgccg acgcggttgg gaaagcgcag gcctgcaagt
     1081 tcgatggggt cgtctacccg gccggcgcca taggccatct tgagcgggtt gccctgggtg
     1141 gcggccagac cggccatggt gaggtcatgg gccacttcgg ggtccatggc aaacaaaaca
     1201 cgacgggcca gaccataagg tacgagagac attggataat tcctgcagtt tttccttaac
     1261 tcccccgatt gtccccgatg accaactccc aaactcctgc acccgccaac gtacttagcc
     1321 aagacgaact caaaaccctg gtcggccagg ccgcgctgca gtatgtagtg cccggcgaga
     1381 tcgttggcgt gggcaccggc tccacggtca acaagttcat cgacgcgctg gccaccatca
     1441 aggaccagat caagggcgcg gtgtccagtt cggtggccag caccgagcgg ctgcaggcgc
     1501 tgggcatttc ggtgttcgat tcgaacgcgg tggaccgcct gtcggtctat atcgacggtg
     1561 ccgatgagat cgatggtgcc ggcaacatgg tcaaaggtgg cggcgcggcc ctgacccgcg
     1621 aaaagattgt ggccgcgcag agcgcgaagt ttgtgtgtat tgccgacgag tccaagctgg
     1681 tgcagacatt gggcaaattc cctctgccgg tggaagtgat tcccatggcc acgcagcgca
     1741 tcattgccca gtttgccgcc aagggtggca ctggcaaggt gcggctgaag gatggcaagc
     1801 cgctggtgac cgacaacggc cagttcatcg tggatgtgac cggtttgcag atcaccgacc
     1861 cgctggcgtt tgaagccgaa gtaagccagt ggcctggcgt ggtgacggtg ggcgtgtttg
     1921 cctaccagcg tgcccaggtg tgcctgttgg gcacggccag cggcgtaaaa actttgacgt
     1981 tctgacgctg accttctgaa cctgctttag aacgtgatgc cggccgggtt ggagggcgag
     2041 ggtcccactg gcggtgctgc ttccacaggt gctggtgctg gctccgcctt gcgttcggct
     2101 ggcttctgca cggcgaccag tggtgtagcc ggtgcattct tgaagccggc tggcagcgtg
     2161 gacgtgcggg ggtagcctgc ggcgtccagg tactgtgacc attccgcgtc ggaatagccc
     2221 ttgaggcgtt gtgagccgat ggtcagcacc ggaatggagt tctcgccggt caggcgctgc
     2281 agtgctgccg tgtcttccgc ggtggtgacg gttcgctcgt tgaacggcac gccgcggctg
     2341 gtcagcaggg agcggccggc accgcacggt gcgcagtcgc tggaggtgta cagaacgacc
     2401 gggtatttgg caaccacctg gcgcagttca aacggcaggt tggtggcaga tgctgcaccg
     2461 gcagcaccta ctccagtggc ggccaccttg ccctggtctg ctgacggggg cttgtccgag
     2521 aacgtgactt ttccgtcggg gccgacgatg cgatagacca cctgggcttg cgcaacggcg
     2581 ctggctccca gcagcgccgc gctggtgagc agtagtgcaa gggtgtgacg tttcatgtcc
     2641 agactccaat aacagatgac aagagtttac gctgccatgc cctggtggcg cagcagggcg
     2701 tccagcgtgg gttcgcggcc gcggaaggct ttgaaggatt ccatggccgg gcggctacca
     2761 ccggcttcca gaatggattc gcggtatttt ctgccggttt ccgcgctggg ggtgccgtcc
     2821 gcagcggcgg tttcctcaaa ggctgcgtag gcatcggcgc taaggacttc ggcccatttg
     2881 tagctgtagt agcccgccgc gtaaccaccg gcaaagatgt ggctgaaggt gtgggcagtg
     2941 cggctccagg caggcgggtg catcacggcc acctcctggc gcacggtgtg cagcaggggc
     3001 aagaaatcca ccgccgggtc gtggctggtg tgcagcagca tgtcgaacag cgcaaattca
     3061 atctggcgca gggtctgcat gccgctctgg aagttcttgg cggccagcat cttgtcaaac
     3121 agggcacgc
//
LOCUS       DAOSJM010000295         3121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27125_length_3121_cov_4.015982, whole genome shotgun sequence.
ACCESSION   DAOSJM010000295 DAOSJM010000000
VERSION     DAOSJM010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27125_length_3121_cov_4.015982"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PKX82_10125"
     CDS             <1..409
                     /locus_tag="PKX82_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52654.1"
                     /translation="LGNLTALENVMLPLELDARKDARKLATEMLGRVGLSTRLNAYPR
                     VLSGGEQQRVALARAFVVHPAVLLADEPTGSLDFATGEKIMELMFDLNREQGTTLVLV
                     THDRGIAQRCDRRITIEAGRVVPEVPVMEVSGL"
     gene            complement(487..786)
                     /locus_tag="PKX82_10130"
     CDS             complement(487..786)
                     /locus_tag="PKX82_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52655.1"
                     /translation="MASNRHVIAVRWHEGKYAVIADQIFNGQDDIDWRALEAKHPWND
                     VSVDTGYQDHASVISIEQLSRYVRSDAARAYVERTKAAGVFLYLIHRAEYETGLG"
     gene            895..1668
                     /gene="rlmB"
                     /locus_tag="PKX82_10135"
     CDS             895..1668
                     /gene="rlmB"
                     /locus_tag="PKX82_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOF52656.1"
                     /translation="MSSPKVLFGFHAVGVRLKTAPKSIVEIYVDPTRRDARMRLFLEK
                     VTESGVRLIEADGVRLAKLAGSHGHQSVAARVQEIKQVHSLDELLENLEAANVALPPE
                     QRTQPLILVLDGVTDPHNLGACLRVADGAGAHCVIAPKDHAAGINATVAKVASGAAET
                     VPYFMVTNLARTLNELKERNIWIIGTSDQATSNLYQADLKGPVALVLGAEGDGMRQLT
                     AKTCDQLVSIPMRGAVESLNVSVASGVCLYEALRQRTAA"
     gene            1690..2049
                     /locus_tag="PKX82_10140"
     CDS             1690..2049
                     /locus_tag="PKX82_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52657.1"
                     /translation="MFAIKSRAACAALAGATALFILSACTQEQQNKISRDIQNWTGTN
                     GVLEIYAGDKVVRRFLKIDKMSTAMGTDDKSPRPYRYGYGILDENFNMQADPGEKKVY
                     FEISDYASNYLFFENPH"
     gene            2059..2562
                     /locus_tag="PKX82_10145"
     CDS             2059..2562
                     /locus_tag="PKX82_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52658.1"
                     /translation="MRPVLAWGSVSLALLVGLGAYLAPLKPSLVALQFTFTPEAFGTV
                     LAAWQPVGVALYRSHLPVDGVLLLSYGWFGYLLASTSPLFAQYAAAHRRSLAWLLPLA
                     AVADSGENLLHWLLTSDGTVHAPWIYALAGACASLKWCGLLVFAAAVLWAWTHRKPGS
                     DPTHPAQ"
     gene            complement(2536..3120)
                     /locus_tag="PKX82_10150"
     CDS             complement(2536..3120)
                     /locus_tag="PKX82_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOF52659.1"
                     /translation="MTAADWLTLWVHFMSLSLLAVGGAITTAPDMHRYLVVEQHWLTD
                     SQFTASIALAQVSPGPNVLFVALLGWNVGLNAAGGLGAGVVAWSYGLLGVLLCMLGIM
                     IPSTTLTFAAARWGHRNRELRAVRAFKQGMAPIVIALLVATGWILASSQGSVQEHWPL
                     WLVTVGAAVLVWKTKLHLLVLLGAGGLLGWMGWV"
BASE COUNT          580 a          949 c         1013 g          579 t
ORIGIN      
        1 gctgggcaac ctgaccgcgc tggagaacgt gatgctgccg ctggaactgg atgcccgcaa
       61 ggacgcgcgc aagctggcca ccgagatgct gggccgtgtg gggctgtcca cccggctcaa
      121 cgcctatccc cgggtactca gcggggggga gcagcagcgt gtggccctgg cccgtgcctt
      181 tgtggtccac cccgcggtcc tgttggccga cgagccgacg ggcagcctgg actttgccac
      241 gggcgaaaaa atcatggagt tgatgttcga tctcaaccgc gagcagggca cgacgctggt
      301 gctggtgacg cacgaccggg gcattgccca gcgttgcgac cggcgcatca ccattgaggc
      361 gggcagggtg gtgccggagg tgccagtgat ggaggtttct ggcctctagc ccccggacaa
      421 taagcgcggg gagctatata aacgatagca gatcaagctg caccctctcc gtggtgtctc
      481 gctgtctcag cccagtccgg tttcatattc ggcccggtgg atcaggtaca gaaatacgcc
      541 ggcggccttg gttctctcga cataggcacg ggctgcatcg ctgcgcacat agcggctgag
      601 ctgttctatg gaaatcacgg aggcatggtc ttggtagccg gtgtcgacgc tgacatcgtt
      661 ccaggggtgc ttggcttcca gcgcccgcca gtcgatgtca tcctggccat tgaagatctg
      721 gtctgcaatg acggcgtatt tcccttcgtg ccagcgcacg gcaatgacgt ggcggttgga
      781 tgccataggt gtgttccggt gcaatagtgc atggtttata gctgaacttg ccgtgcaaac
      841 ggaaaacacc ggtctgctgg ccaggtgcgc tgccttagcc ccgataatca gggcatgtct
      901 tcccccaaag ttctcttcgg cttccatgcc gtaggcgtgc gcctgaaaac cgcacccaaa
      961 tccatcgttg aaatctatgt ggaccccacg cggcgcgatg cacgcatgcg cttgtttctg
     1021 gagaaagtca ccgagagcgg cgtgcgtctg atcgaggccg atggtgtgcg tctggccaaa
     1081 ctggcgggca gccacgggca ccagagcgtg gccgcacgtg tgcaggaaat caagcaggtg
     1141 cattcgctgg acgaactgct ggagaacctg gaggcggcca acgttgccct gccgccggag
     1201 cagcgtacgc agcccctgat tctggtgctc gacggcgtga ccgatccgca caacctgggt
     1261 gcctgcctgc gcgtggccga tggcgccggc gcccactgcg tgattgctcc caaggaccat
     1321 gcggccggca tcaacgcgac agtggccaag gtggccagtg gtgcggccga gacggtgccg
     1381 tacttcatgg tgaccaacct ggcacgcact ctcaatgagc tcaaggagcg caatatctgg
     1441 atcattggca ccagcgacca ggccaccagc aacctctacc aggccgacct caagggcccg
     1501 gtggccctgg tgctgggtgc cgagggcgat ggcatgcgcc agctcaccgc caagacctgc
     1561 gaccagctgg tgagcattcc catgcgcggt gcggtggaga gcctgaatgt gtcggtggcc
     1621 agtggcgtct gcctgtacga ggcactgcgc caacgcactg cagcctgaat tggccgaagg
     1681 gggaatacca tgtttgctat taaatcgaga gctgcttgcg cagcattggc gggggctaca
     1741 gccctattta ttctgagtgc gtgcacccag gagcagcaga acaagatcag ccgcgacatc
     1801 cagaactgga ctggcaccaa cggcgtgctg gagatctatg ctggcgacaa ggtggtgcgc
     1861 cgattcctga agatcgacaa gatgtccacc gccatgggga ccgatgacaa gagtccgcgt
     1921 ccttaccgct atggctacgg catcctggat gaaaacttca acatgcaggc ggatccgggc
     1981 gagaagaagg tgtacttcga gatcagcgat tacgccagca actacctgtt ctttgaaaat
     2041 cctcactagg ccatcgccat gcgcccggtc ttagcctggg gatcggtgtc actggccttg
     2101 ctggtcgggc tgggtgcgta ccttgcgcca ctcaagccca gtctggtggc attgcagttc
     2161 acattcacac ctgaagcctt cgggaccgta ctggcagcgt ggcagcccgt gggggtggca
     2221 ctgtatcggt cgcatttgcc ggtcgacggt gttctgctgt tgagttacgg gtggtttggc
     2281 tatctgttgg cttccacgtc tcccttgttt gcgcagtacg ccgcagccca ccggcgcagc
     2341 ctggcctggc tgctgccttt ggcagccgta gccgactccg gggagaacct gctgcactgg
     2401 ctgctgactt ctgacgggac cgtgcacgcg ccgtggatct acgcactggc aggcgcctgt
     2461 gccagtctca agtggtgcgg actgctggtg tttgcggccg cggtgctatg ggcgtggacc
     2521 catcgcaagc ctggctcaga cccaacccat ccagcccagt agaccgcctg cgcccagcaa
     2581 caccagcagg tgcagtttgg tcttccagac caggacagcg gcgccaacgg tgaccagcca
     2641 caaaggccag tgttcctgca cactgccctg gctgctggcc agaatccagc cggtggccac
     2701 caacagtgcg atcacgatgg gggccatgcc ctgcttgaag gcgcgcacgg cgcgcagctc
     2761 acggttgcgg tggccccagc gggcggcggc aaaggtgagc gtggtgctcg gaatcatgat
     2821 gcccagcatg cacaggagta cgcccagcag gccgtagctc caggcgacca ctccggcacc
     2881 caggccgcca gcggcattca gaccgacgtt ccagcccaac aaggcgacga acagcacatt
     2941 gggtcccggt gaaacctgcg ccagtgcgat cgaggcggtg aactggctgt cggtcagcca
     3001 gtgctgttcg accaccaggt agcggtgcat gtccggcgcg gtggtgatgg cgccgcccac
     3061 ggccagcagc gacagggaca tgaagtgcac ccacaaggtg agccagtcgg cggcggtcag
     3121 t
//
LOCUS       DAOSJM010000296         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27322_length_3105_cov_3.013770, whole genome shotgun sequence.
ACCESSION   DAOSJM010000296 DAOSJM010000000
VERSION     DAOSJM010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27322_length_3105_cov_3.013770"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /gene="aztA"
                     /locus_tag="PKX82_10155"
     CDS             complement(<1..718)
                     /gene="aztA"
                     /locus_tag="PKX82_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HOF52660.1"
                     /translation="MSALPAARAAISLHNLTVSYRQHPALHHVSGHFAQGSLTAVIGP
                     NGAGKSTLLKGIAGLVPLDRHARIERAPDLRMAYLPQHSELDRSFPLDVRDCVLMGLW
                     TQTGAWGRATAAMLARVDAALEAVGLQGFGHRPVGTLSSGQLQRTLFARLLVQDAQII
                     LLDEPFNAVDSKTTASLLTLVQQWHRQGRTVVAVVHDDAQVREHFPQTLLLARECMAW
                     GATAEVLTHENLARARNMAEA"
     gene            complement(715..864)
                     /locus_tag="PKX82_10160"
     CDS             complement(715..864)
                     /locus_tag="PKX82_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52661.1"
                     /translation="MTTPRAPAQLYKQLSCIALPAWKRVALTLPALALLWLGVWWASL
                     EAAPL"
     gene            938..1465
                     /locus_tag="PKX82_10165"
     CDS             938..1465
                     /locus_tag="PKX82_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOF52662.1"
                     /translation="MASALPPAAPVLKGDDPIDALLSAHGLRRTVAARRVLGWLLAHP
                     DTSYTHAQLQDALAGSSAEPLDRVTLYRLIDRLTQVGLLLCRVDSQRVRRYQAMPASV
                     HATPHFECQSCHRDSPLAGALRASDLEKAAETAIEALRALGYQDMHLDFAVRGVCADC
                     ATGATATGATAGPAA"
     gene            1462..2160
                     /locus_tag="PKX82_10170"
     CDS             1462..2160
                     /locus_tag="PKX82_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52663.1"
                     /translation="MIRTQGLAYQYPGTPNAAVLQFPDVDVAQGAVLLLSGPSGCGKS
                     TWLALVAGLVAPSAGTVEVVGQPLHALKSVASDAWRARTIGFLPQKLHLSAALTVQQN
                     LAMAQWAAGQSEDSVAIHSALQALGVDELVQRTPAQLSGGQAQRVALARAVLLQPRVL
                     LADEPTASLDDEAAADAVALLLSTARTHGATLVIATHDARVAALIPAGVEGQIGFQPL
                     QLLRKQLSKEERSA"
     gene            2157..>3105
                     /locus_tag="PKX82_10175"
     CDS             2157..>3105
                     /locus_tag="PKX82_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF52664.1"
                     /translation="MRMLRFAWRYLWSRPLGAALNVLLLSLGLASITFLLLVGAQLSK
                     AFDRDLAGIDVVVGAKGSPMQLILSGVLHIDVPPGNVPFSAVKELARNPLVDHIIPIS
                     LGDNYAGYRIVGTSLAYLDHYEAQLAQGPQNRLWDAPMQVVLGATTARKLGLAPGQTF
                     VGSHGLGAGGHTHGDIVYTVVGILAPSGSVLDRLILTDTASVWKVHEDYTATDDEDRQ
                     VLEEEREITMALVQYKTPMAALSFPRYVNTSTEMQAAAPALEISRLLHMLGLGTEVLR
                     AFAGVLLLTAGLSVFIALWSAVRERRADLARRRTQRAQQR"
BASE COUNT          509 a         1017 c         1029 g          550 t
ORIGIN      
        1 aggcctcggc catattgcgt gcgcgcgcca ggttttcgtg ggtcagcacc tcggccgttg
       61 cgccccaggc catgcactct cgcgccagca gcagcgtttg tgggaagtgc tcccgcacct
      121 gcgcatcgtc atgcaccacg gccaccacgg tgcggccctg acggtgccat tgctgcacca
      181 gcgtcagcag gctcgccgtg gttttgctgt ccaccgcgtt gaagggttcg tccagcagga
      241 tgatctgtgc gtcctgcacc aggaggcgcg caaacagtgt gcgctgcagc tgtccgctcg
      301 aaagtgtgcc taccgggcgg tgcccaaaac cctgcagtcc cacggcctcc agtgcggcgt
      361 ccacccgtgc cagcatggct gcagtggcgc ggccccaggc acctgtttgg gtccacaaac
      421 ccatgagcac acagtcgcgt acatccagcg gaaagctgcg gtccagttcg ctgtgctgcg
      481 gcaggtaggc catccgcaga tcgggtgcac gttcgatgcg ggcatgccgg tccagtggca
      541 ccagaccggc gatgcctttg agcaacgtgc ttttgcctgc gccattggga ccgatgacgg
      601 cggtgaggga gccctgcgca aagtggccac tcacatggtg cagggccggg tgttggcggt
      661 aactcaccgt caggttgtgc agggatatgg cagcccgtgc tgctggcaac gcactcatag
      721 cggtgcagcc tccaggctgg cccaccacac gcccagccac agcagggcca gcgcgggcag
      781 ggtgagggcc acccgcttcc acgctggcag ggcgatgcaa ctcagctgtt tgtaaagttg
      841 ggcaggggcg cgtggagtgg tcacaaagct atctcataat gcaactggat tgcgatatag
      901 tctaacgcaa cttagttgca tttgtaggga tgctttgatg gcttctgcac tgcctccggc
      961 tgcaccggta ttgaagggtg atgacccgat cgacgcgctc ctgtcggccc acggcctgcg
     1021 ccggaccgtg gcggcgcgcc gggttcttgg ctggttgctg gcccatcccg acaccagcta
     1081 cacccatgcc cagctgcagg acgcactggc aggcagcagt gccgagcccc tggaccgggt
     1141 cacgctgtac cggctgattg accgcctgac ccaggtcggc ctgctgctgt gccgtgtgga
     1201 cagccagcgc gtgcgccgct accaggccat gccggccagc gtgcatgcca ccccgcattt
     1261 cgaatgccag agttgccacc gcgacagccc cctggccgga gccctgcgcg ccagtgacct
     1321 ggagaaggcg gccgaaaccg ccatcgaagc actgcgcgca ctgggctacc aggacatgca
     1381 tctggacttt gccgtgcgcg gtgtgtgtgc cgactgcgca accggcgcca cggcgaccgg
     1441 ggccaccgca gggccagctg catgatccgt acccagggtc tggcgtacca ataccccggt
     1501 acccccaacg cggcggtgct gcagtttccc gatgtcgatg tggcgcaggg cgccgtgttg
     1561 ctgctcagtg gcccctctgg ctgcggcaag tcgacctggc tggcgctggt ggccgggctg
     1621 gtagcgccca gcgcgggtac cgtggaggtt gtgggtcagc ccttgcatgc tctcaagtcc
     1681 gtggcgtctg acgcatggcg ggcgaggacg atcggctttt tgccccaaaa attgcacttg
     1741 agcgcggcgc tcaccgtgca gcagaacctg gccatggcgc aatgggccgc tggacagtcc
     1801 gaggacagcg ttgccatcca ctctgcactg caggccctgg gcgtggacga gctggtgcaa
     1861 cgcacacccg cacagctctc cggcgggcag gcacagcgtg tggctctggc acgtgcggtg
     1921 ctgttgcagc cccgcgtgtt gctggccgac gagcccaccg ccagcctgga cgacgaggcc
     1981 gcggccgacg cggtggccct gctgttgagc acggcccgca cccacggtgc cacgctggtc
     2041 attgccaccc acgatgcgcg cgtggcagcg ctcattcctg ccggggttga gggtcaaata
     2101 ggcttccagc ccttgcagct attgcgcaag cagctatcaa aagaggagcg gtccgcatga
     2161 ggatgctgcg ctttgcctgg cgctacctgt ggtcgcgccc gctcggggcg gcactcaatg
     2221 tactgctact gagcctgggg ttggcgtcca tcacctttct gctgctggtg ggggcgcagc
     2281 tcagcaaagc atttgaccgc gacctggccg gcattgacgt ggtggtgggg gccaagggca
     2341 gccccatgca gctgattctg tcgggtgtgc tgcacatcga tgtgccgccc ggcaatgtgc
     2401 cgttcagcgc ggtcaaggag cttgccagga acccgctggt ggaccacatc attcccatca
     2461 gtctgggcga caactatgcg ggctaccgca tcgtcggcac ctcgcttgcc tatctcgatc
     2521 actacgaagc ccaactggcc cagggcccgc agaaccgtct atgggatgcg cccatgcagg
     2581 tggtgctggg cgccaccacg gcgcgcaaat tggggctggc gccggggcaa acctttgtcg
     2641 gctcgcacgg cctgggcgca ggtggccaca cgcatggtga catcgtctac accgtggttg
     2701 gcatcctggc acccagcggc agcgtgctgg accgcctcat cctgaccgac accgcttcgg
     2761 tctggaaggt gcacgaggac tacacggcaa cggacgatga agaccgacag gtgctggaag
     2821 aagaacgcga aatcaccatg gcgctggtcc aatacaaaac cccaatggcc gcactgagtt
     2881 ttccgcggta cgtaaacacc agtaccgaga tgcaggccgc tgcccccgcg ctggagatca
     2941 gccgcctgct gcacatgctg ggtctgggca ccgaggtgct gcgtgccttt gccggtgtgc
     3001 tgctgctgac cgcgggcctg agtgtcttta tcgcgctgtg gagcgcggtg cgcgagcgcc
     3061 gtgccgatct tgcccggcgg cgcacccagc gtgcgcagca gcgca
//
LOCUS       DAOSJM010000297         3092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27471_length_3092_cov_3.523872, whole genome shotgun sequence.
ACCESSION   DAOSJM010000297 DAOSJM010000000
VERSION     DAOSJM010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27471_length_3092_cov_3.523872"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PKX82_10180"
     CDS             complement(<1..188)
                     /locus_tag="PKX82_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOF52665.1"
                     /translation="MSFPLDTDTVSHGIQLAVAPVFLLTAVSGMIGVVANRLARIIDR
                     ARVLEQRLKDTPTEADTS"
     gene            371..1030
                     /locus_tag="PKX82_10185"
     CDS             371..1030
                     /locus_tag="PKX82_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOF52666.1"
                     /translation="MKRLFLLAASLYATTLACAQDMGRVLSSTPVMQQIAVPRQVCST
                     EQIEVQRPNSGAGAAIGAIAGGALGSASGHGPGRAAATVIGAIGGAVLGDRIEGTPEP
                     QFQNVQRCSMQTFFETRTVAYNVVYEYAGKQYSVQMPNDPGPNIALQIAPAGASAPAT
                     SPAPQVVQQTPVYVQPEPLIVTRQFYPYYAPRPYFAPVFIDLGVGYRGGHPGHGRRHW
                     R"
     gene            complement(1027..1431)
                     /locus_tag="PKX82_10190"
     CDS             complement(1027..1431)
                     /locus_tag="PKX82_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOF52667.1"
                     /translation="MAYFDWADDMVIDGGAIDADHRRLVDLVNALHTATTEGRGREVV
                     EGIMKELLEYTTAHLRREEAQMASVGFPHLERHKIGHDHFVAQLAELQKKYEAGSITV
                     AAQLSTVLRDWLSLHIRRSDKELKSYVEKLGR"
     gene            complement(1447..3015)
                     /locus_tag="PKX82_10195"
     CDS             complement(1447..3015)
                     /locus_tag="PKX82_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017774803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOF52668.1"
                     /translation="MKIGVRMGAGFGLLILMMLVMALVSVMRFGDVAEINDRIIEKDW
                     VKAEAANVINATTRANARRTMELVIATDPQQINSVKQNIETNKKTISAALDTLESLIY
                     LPEGKALVARLKEARGKYVASFSKVAKLVDEGKRDEAITVLNAETLPALDALQEPINA
                     LNELQRKIVVASSAELRAKTASARTLLLVLGIFGLVLGAGFSYWLTVSVTRPLHEAVH
                     MTKTVASGDLTVQVGAHSKDEAGQLLTALQDMNDSLVNIVHEVRNGVDSMATATSQIA
                     AGNMDLSSRTEQQASALEQTAASMEELAETVKQNFESGRHANQLADSAAAVAVRGGDV
                     VARVVHTMEAINTSSNKIADIIGLIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAS
                     EVRSLAGRSAAAAKEIKDLIGASVSNVSDGCKLVEQAGSTMDEIVVGVRRVADLMREV
                     TMASQDQTSGIEQVNQAVGQMDQVTQQNAALVEEAAAAAQSLEHQAQSLLHTVSVFRV
                     GAGSSRMRSLPSPA"
BASE COUNT          605 a          979 c          828 g          680 t
ORIGIN      
        1 aagctggtat ccgcctctgt gggggtgtcc ttcagtcgct gctccagcac ccttgcacga
       61 tcgatgatgc gcgcaaggcg gttggcaacc accccgatca ttccagacac agcagtgagc
      121 aggaatacgg gagcaactgc cagctgaatg ccatgggaca ccgtgtcagt atctagtgga
      181 aaactcatgc tctactttct ttgggatgat gcagtaccta cagggcgcgc tttatcctga
      241 ttctgacagc tgtgtaaagt tcgcaacgaa tccagcccct ctgtcatcca actagactgc
      301 atcaaccgtt acagatggca ttgggggtta atcctccctc gcgggctgta tggatgaatt
      361 tgggcacacc atgaagagac tattcttgct agcggcgtcg ctgtatgcca ccacactggc
      421 ctgcgcgcag gacatgggcc gcgtactgtc gtccacacct gtcatgcaac agatcgctgt
      481 accgcggcag gtctgcagca ccgagcagat cgaagtacag cgccccaact caggtgctgg
      541 tgctgctatc ggagccatcg cggggggcgc actgggcagc gcttcgggcc acggccctgg
      601 acgcgcagcc gccacagtga ttggtgccat tggcggagcg gtcctgggcg accggataga
      661 aggcacgccg gaaccccagt tccagaatgt gcaacgctgc tccatgcaaa cgttctttga
      721 gacacgcacc gtggcttaca acgtggtgta tgaatacgct ggcaagcaat attcggtgca
      781 gatgcccaat gaccccggtc cgaatatcgc gctgcagatt gcccccgcag gcgcgagtgc
      841 tcctgcaact tcaccagcac cgcaagtggt gcaacagact ccggtctatg tgcagccgga
      901 acccctcatc gtgacgcggc agttttatcc gtactacgcc cccaggccct actttgcccc
      961 tgtttttatc gatttgggcg ttggctatcg gggtggccac ccgggccacg gtcgcaggca
     1021 ttggcgctag cgccccaact tctccacgta gcttttcagt tccttgtcgg aacgccggat
     1081 atgcaatgac agccagtcac gcaatacggt ggaaagctgt gccgcaacgg tgatactgcc
     1141 agcttcatac tttttctgaa gctcagctag ttgcgctacg aaatggtcat gcccgatctt
     1201 gtggcgctcc aggtgcggaa aaccgaccga tgccatctgc gcttcttccc ggcgcaaatg
     1261 tgcagtggtg tactccagca attctttcat gatgccttca accacttcac gcccgcggcc
     1321 ctctgtcgtc gccgtgtgca gtgcattgac cagatcaacc agcctgcggt gatcggcatc
     1381 gatggcgccg ccatcaatga ccatatcatc cgcccaatca aagtaggcca tggtcgtgct
     1441 tccttctcaa gcaggcgagg gcagcgaacg catccggctc gaccccgctc ccacgcgaaa
     1501 gacgctgacg gtgtgcaaca gcgactgggc ctgatgctcc agcgattggg cagccgcggc
     1561 cgcctcttcc accagcgcag cattctgctg ggtcacctgg tccatctggc ccacggcctg
     1621 gttgacctgt tcgatgccgg acgtctgatc ctggctggcc atggtcactt cgcgcatcag
     1681 gtccgcaacg cggcgcacgc ccaccacaat ttcgtccatg gtgctgccgg cctgttccac
     1741 cagcttgcag ccatcgctga cattgctgac ggacgcacca atcaggtcct tgatttcctt
     1801 ggccgctgcc gccgatcggc cggccaggct gcgcacctca ctggcgacca ctgcgaatcc
     1861 acgcccctgt tcacccgcac gcgcagcctc aacggcggcg ttgagtgcca ggatattggt
     1921 ctgaaatgca atgccatcta tcaacccgat gatgtctgca atcttgttgg acgaagtatt
     1981 gatcgcctcc atcgtatgca ccacgcgggc caccacatca cccccacgca ccgccacagc
     2041 cgccgccgaa tcggccaact ggttggcatg ccgcccgctt tcgaagtttt gtttgaccgt
     2101 ttcggccaac tcttccatcg acgctgcggt ttgttcgagt gcactggcct gctgctccgt
     2161 acgtgacgac aagtccatat tgccagcggc tatctggctg gtggcggttg ccatgctgtc
     2221 cacaccattg cgtacttcat ggacgatatt gacgaggctg tcattcatgt cctgcaacgc
     2281 cgtcagcagt tgcccggctt catccttgga atgcgcaccc acttgcaccg tgaggtcacc
     2341 cgaagcgacc gtctttgtca tgtgaacagc ctcgtggagg gggcgcgtca cagataccgt
     2401 caaccagtag gaaaagccag cccccaagac cagcccaaaa atgcccaaaa ccagcagcaa
     2461 cgtccgtgcg ctggcggttt tggccctgag ctctgcactg ctggcaacca caattttccg
     2521 ctgcagttcg ttgagcgcat tgatgggctc ctgcaatgca tccagcgcag gaagggtttc
     2581 tgcattgagc acggtgatgg cctcatcgcg ctttccctca tccaccagtt tggccacttt
     2641 cgagaacgat gccacatact ttccgcgcgc ttccttgagc ctggctacca gggctttgcc
     2701 ctccggaagg tagatgagcg actccagcgt gtccagcgct gctgaaatgg ttttcttgtt
     2761 ggtttcgatg ttttgcttga cactgttgat ctgctgggga tccgtagcaa tcaccagttc
     2821 catggtacgc cgggcattgg cgcgggtcgt ggcgttgatg acgttggcgg cctctgcctt
     2881 gacccagtct ttctcaatga tccggtcatt gatctctgcc acatcaccga agcgcatcac
     2941 gctgaccagc gccattacca gcatcatcaa aatcagcagg ccaaagcctg cacccatccg
     3001 aactccgatt ttcaagccat tgaatgccac ggaagttccc tcctgaatta cattggaccg
     3061 cttgcatggg cctcactatg ctggaaagcg at
//
LOCUS       DAOSJM010000298         3082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27592_length_3082_cov_4.357450, whole genome shotgun sequence.
ACCESSION   DAOSJM010000298 DAOSJM010000000
VERSION     DAOSJM010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27592_length_3082_cov_4.357450"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PKX82_10200"
     CDS             <1..1065
                     /locus_tag="PKX82_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOF52669.1"
                     /translation="AFSHGPLRMVPGLEDYLDLPAGSNPRTRQLAADMRADPRLTRAD
                     GATLVAAVLERLRTGGYTYTLEPGVFGIHTADEFWFDRKEGFCEHIASSFVLLMRALG
                     VPARIVTGYQGGEVNSVDGYWTVRQSDAHAWSEVWLAGQGWVRVDPTSAVAPGRTGSF
                     QRLAAPTNVFAQALGTVSPSFALNLRAMWDAVNNRWNQWVLNYSQAKQFDMLRNLGFA
                     SPSWEDLTYVLIAIVVSVSLLGAAWTLWERSRQDPWLRLLHRAARKLQDAGLSIPANA
                     APRQLAALVQTASHADAPQRTAITQWLLRLEAWRYAPAKTGGQGAAHAARSLGTLQRE
                     FGQLPWPAKQPQHNAPPDSP"
     gene            1233..2180
                     /gene="mltB"
                     /locus_tag="PKX82_10205"
     CDS             1233..2180
                     /gene="mltB"
                     /locus_tag="PKX82_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HOF52670.1"
                     /translation="MQLADDIAQKRNLDRTWVREAVGQSCYLAGIAKAVTPPPVGVPK
                     NWALYRSRFIEPVRVRAGVKFWQANAEVLQRAQDETGVPAEIIVGIVGVESIYGQQTG
                     TYRVMDALTTLALDFPPAHPRASARSAFFRAELEEFLALTARTRTNPLALLGSYAGAM
                     GMPQFMPSSWVKYAIDFDSDGRVDLFNSSADVIGSVANYFKSFGWKSGMPTHYAVQFD
                     PQTLDMAALMTPDILPTFVAEAFSAKGALLDAQAQQHAGPLALVELQNGDAPPQYVAG
                     TENFYVITRYNWSSYYAMAVIELGREVAAARNSVVDVQK"
     gene            2184..>3082
                     /locus_tag="PKX82_10210"
     CDS             2184..>3082
                     /locus_tag="PKX82_10210"
                     /inference="COORDINATES: protein motif:HMM:NF022400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HOF52671.1"
                     /translation="MVTAAPATDATSATTLDRSETSATAELYRAAIGSIHTDDYLLVF
                     ERFEAADRAGPSWNWAAALLTLNWMAFRRLWGAALAYLGLLATAGLLLFGIGRLVFGL
                     SADVQWALLALLVLLAIAVPGVWGHALLYKACRKRMARALAANDTVEQACAMLRKDAT
                     TRQRLIGMVVANLALAGTAIVAYLNFPDFHDGPLNTAKMGEARTSVQGQVVIEAPTPQ
                     RAASAPQSPASAPDLAASAPVAAASAPAPTASEPVLPALSPAVTVAVTVAPDPAPVSE
                     PVRATPKPEEARPKQPPKQPPKQ"
BASE COUNT          551 a         1081 c          943 g          507 t
ORIGIN      
        1 gccttcagcc acggcccgct gcgcatggtg cccgggctgg aagactacct ggacctgccc
       61 gccggcagca atccgcgcac ccgccagctc gccgccgaca tgcgtgcgga cccacgcctg
      121 acccgcgccg atggtgccac cctggtggcc gccgtcctgg agcgcctgcg caccggtggc
      181 tacacctaca cgctggagcc gggcgtgttt ggcatccaca ccgccgacga attctggttt
      241 gaccgcaaag aaggtttttg tgaacacatc gcgtcgagct ttgtgctgct gatgcgcgcg
      301 ttgggcgtgc ctgcgcgcat cgtgaccgga tatcaggggg gcgaagtcaa ttccgtggac
      361 ggctactgga ccgtgcgcca gagcgatgcc catgcctggt ctgaagtgtg gctggcaggc
      421 cagggctggg tgcgcgtgga ccccacttcg gccgtcgcgc cggggcgcac cggcagcttc
      481 cagcggttgg ccgcacccac caatgtgttt gcgcaggcgc tgggcaccgt cagccccagc
      541 tttgcgctga acctgcgtgc catgtgggac gcggtcaaca accgctggaa ccaatgggtg
      601 ctgaactatt cccaggccaa acagttcgac atgctgcgca acctgggctt tgcatcaccg
      661 agctgggaag acctcaccta cgtgctgatt gccatcgtgg tgtcagttag cctgctgggg
      721 gccgcctgga cgctgtggga acgcagccgc caggacccct ggctgcggct gctgcaccgc
      781 gctgcccgca agctgcagga cgccggcctg tcgatccctg ccaacgccgc gccgcgccag
      841 ctggcggccc tggtgcaaac cgcctcccac gccgacgcac cgcagcgcac cgcgatcacc
      901 caatggctgt tgcggctgga agcctggcgc tatgcgccgg ccaaaaccgg tgggcagggt
      961 gctgcacatg ctgcaaggtc cctcggtaca ctgcagcgtg aattcggcca gttgccctgg
     1021 cccgccaaac agccccaaca caacgcgcct cctgattccc cgtgaaattc tcctccctcc
     1081 caattgctat tatctttata gctagctgcg ccctatccgc gggggctagc acccaaaaac
     1141 acccaaaatc ccacgcaaaa aaggtgtcct acaccaaggt gcgcaaggag ccggcgcgta
     1201 ccgggccgct ctacgccacc cggcccgatg ccatgcaact ggccgacgac atcgcgcaga
     1261 aacgcaacct ggaccgcacc tgggtgcgcg aggccgtcgg ccagtcctgc tacctcgctg
     1321 gcatcgccaa agccgtcacc cccccacctg tcggagtccc gaaaaactgg gcgctgtacc
     1381 gcagccgctt catcgagccg gtgcgcgtgc gtgcgggtgt gaagttctgg caggccaacg
     1441 ccgaggtgct gcagcgcgcg caggacgaga ccggcgtgcc ggcagaaatc attgtgggca
     1501 tcgtcggcgt cgaatccatc tacggccagc agaccggcac ctaccgcgtc atggacgcgc
     1561 tgaccacgct ggccctggat tttccgccag cccatccacg cgccagcgcg cgctccgcct
     1621 tcttccgcgc ggagctggag gagttcctcg cgctgacggc acgcacccgc accaacccgc
     1681 tggcgctctt gggcagctat gccggtgcta tgggcatgcc gcagttcatg ccgtcgagct
     1741 gggtgaagta cgccatcgac tttgacagcg atggccgtgt ggacctgttc aacagcagcg
     1801 ccgatgtcat cggctcggtg gccaactact tcaagtcctt cggctggaag tcgggcatgc
     1861 ccacgcacta cgcagtgcag ttcgacccgc agaccctgga tatggcggcg ctgatgacac
     1921 ccgacatcct gcccaccttc gtagccgaag cattcagtgc caagggggcc ttgctggacg
     1981 cccaggcgca gcaacatgcc ggcccgctgg ccctggtgga actgcaaaac ggcgacgcac
     2041 caccgcagta cgtggccggc accgaaaact tctacgtcat cacccgctac aactggagca
     2101 gctactacgc gatggcggtg atagaacttg gtcgcgaggt ggccgcagcc cgcaacagcg
     2161 tggtggacgt gcaaaaatag cgcatggtta cagcagcccc tgccaccgac gccacctcgg
     2221 ccacgacact ggaccggtcg gaaaccagcg ccaccgccga gctgtaccgc gcggctatcg
     2281 gcagcatcca taccgacgat tacctgctgg tcttcgaacg ttttgaagcc gccgaccgtg
     2341 ccggccccag ctggaactgg gccgcagcac tgctgacgct aaactggatg gcgtttcgcc
     2401 ggctgtgggg ggctgcactg gcgtacctgg gcctgctcgc cactgcaggg ctgctgttgt
     2461 ttggcattgg gcgtttggtg ttcggtctgt ctgccgacgt gcaatgggct ctgctggccc
     2521 tgctggtgct actggcgatt gccgtgccgg gcgtgtgggg acatgcactg ctctacaagg
     2581 catgtcggaa gcgtatggcc agggcactgg cggccaacga cacggtggag caagcctgcg
     2641 caatgctgcg caaagacgcc acgacacgcc agcgcctgat aggaatggtt gtggccaacc
     2701 tggccctggc tggcacggcc atcgtcgcgt acctgaactt cccggacttc cacgacggcc
     2761 ccctgaacac tgcaaaaatg ggcgaagcac gtaccagcgt gcaaggccag gtggtcatcg
     2821 aagcacccac gccccagcgc gcggcgtctg ccccccagag tccggcatcg gccccggacc
     2881 tcgcggcatc cgcgcccgtt gcggcagcat cggcgccagc gcccactgca tccgagcccg
     2941 ttcttcctgc tttatcgcca gcagttaccg tagcggtgac cgtggctcca gacccggctc
     3001 cagtgtccga gcccgtgcgc gccacaccca aaccggaaga ggcacgcccc aaacaacctc
     3061 ccaaacaacc tcccaaacaa ca
//
LOCUS       DAOSJM010000299         3080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27608_length_3080_cov_3.737851, whole genome shotgun sequence.
ACCESSION   DAOSJM010000299 DAOSJM010000000
VERSION     DAOSJM010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27608_length_3080_cov_3.737851"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="PKX82_10215"
     CDS             <1..1171
                     /locus_tag="PKX82_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF52672.1"
                     /translation="SERLHPDVLKLGLVSFLTDLSSEMIFSVFAVFFTTVAGASSALL
                     GLIEGLADFSASSLNYLSGWLSDRSGQRKWFATAGYGFSTLAKLILLVSSSIVGLSIF
                     RVIERLGKGFRGPPRDAWLSSIAVKENRGYAFGVHKALDKSGAVLGPLVAYGLLSWLG
                     ESASTYSTLFLVAFIPAVVSVAVLARMPDQPGQVHPRESIVQNWALLSPGFKRFLWPA
                     GVFALAYFSLGFILIKAHDVGFSVTEIVLLYALFNTTCVVAAPMVGKLGDRVGRSRIV
                     VLGYAVYAGISLWLMVASTRWELIAAFAMYGLFYSIEESQSKAFIADLEPDRRATAVG
                     VYNMVTGVLYLPASLVAGALWGVAPALAFAAAAALALAAIGVFWLQRPATAARRT"
     gene            complement(1149..>3080)
                     /locus_tag="PKX82_10220"
     CDS             complement(1149..>3080)
                     /locus_tag="PKX82_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52673.1"
                     /translation="LHQQISPDLKAKPSAAITDINEAQRDAIWRSVAAMRKEGIYTTL
                     SPYWAVPMKFAAGWNFPGGSEQSATGLLFFDPMLQHAYKAWLRKLLTEKNPYTGVALV
                     NDPSVAILQLQNEDSLLFWSFNHIQGAQRQALERLFATFLARKYGTLAKVQDAWAGAQ
                     ETGDAPQDGRMTLLNLWELTQPSRDWPLVGDPKDLRGGKAVRRADQTEFLARTMHDFN
                     ATMVRFLRDELGAKQLINAGNWKTGSTELLNDAERWSYTAADVDAANVYNGGIHKGPK
                     NGWAILAGDQFTNESILRTPQLLPIHLKQTQGRPMLLTEGNWVMPNNFGAEGPFLIAA
                     YASLTGVDGYYWFSTGDEGWTPPQSANGYLPNSQGRWIFATPEILGSFPAAALAYRMG
                     YIAKGTPVLQENRSLQDLWQRKPPLLSESTSFDPNRDSGDRAANAERKGGLPGSAFLV
                     GPALVSLGKEGNAPSRAADLAPWVTPQGVQSSTAQLVWNTQQGFCTINTPQVQGVAAH
                     FANAPVHQLKDVRFASGNTFGAAMAVSMDGLPLQQSRRILLQYATQSRPTGWRETPAA
                     IALEGGQQASGFTVQAVGHSPWQVHSAQLEVTVNNPHVSTATVLDMNGMAARTLDLKR
                     ASGSASLRFPADAMYVVLQ"
BASE COUNT          469 a          919 c         1027 g          665 t
ORIGIN      
        1 aagtgaacgg ctgcacccgg atgtgctcaa gctcggactg gtgagctttc tcaccgatct
       61 gagttccgag atgatctttt cggtgttcgc cgtcttcttc accacggtgg cgggggcatc
      121 gagtgcgctg ttgggcctga tcgaggggct ggccgatttt tcggcgtcct cgttgaacta
      181 tctctctggc tggctgtcgg accgcagcgg gcagcgcaaa tggtttgcca ccgccggcta
      241 tggtttttcc acgctggcca agctgatatt gctggtgtca tcgagcattg tgggcctgag
      301 catctttcgt gtgatcgagc gcctgggcaa gggctttcgc ggaccgccgc gggatgcctg
      361 gctgtcgtcg attgccgtga aagaaaaccg cggctatgcc tttggcgtgc acaaggccct
      421 ggacaaatca ggcgcggtgc tcgggcctct ggtggcctac ggtctgctgt cctggctggg
      481 ggagtcggcc agcacctatt cgacgctgtt cctggtggcc ttcattcccg ctgtggtcag
      541 tgtggccgtg ctggcccgca tgcccgacca accgggccag gtgcacccgc gtgaaagcat
      601 cgtgcagaac tgggcgctgc tcagccccgg gttcaagcga ttcctgtggc ctgcgggtgt
      661 gtttgcgctg gcgtatttca gcctcggatt tatattgatc aaggcccatg acgtgggctt
      721 tagcgtgacc gagatcgtgc tgctgtacgc cctgttcaat acgacctgtg tcgttgccgc
      781 gccgatggtg ggcaagctgg gcgaccgggt ggggcgcagc cgcattgtgg tgctgggtta
      841 tgcggtctat gcgggtatca gtctgtggct gatggtggcg agtacgcgct gggagctcat
      901 tgccgcgttt gccatgtacg gtcttttcta ttccattgag gaatcccaaa gcaaggcctt
      961 tatcgccgac ctcgagccgg accggcgcgc gacggcggtg ggggtctaca acatggtgac
     1021 cggtgtgctg taccttccgg catctctggt ggccggtgcg ctttggggcg ttgcccccgc
     1081 gctggcattt gccgcagcgg cggcactggc gctagctgcc atcggagtct tctggctgca
     1141 gcggcctgct actgcagcac gacgtacata gcatccgccg gaaaacgcag gctggcgctg
     1201 ccggatgcgc gcttcaaatc cagggtgcgc gccgccatac cgttcatgtc cagcacggtg
     1261 gccgtgctga catgggggtt gttcaccgtc acctccagct gtgcgctgtg tacctgccag
     1321 ggcgaatgac ccactgcctg caccgtgaat ccgctggcct gttgcccccc ttccagtgca
     1381 atggctgctg gtgtttcgcg ccagccggtg ggtcggctct gcgtggcgta ctgcagcagg
     1441 atgcgacggg attgctgcaa cggcaaaccg tccatcgaca ccgccatggc ggcaccaaag
     1501 gtgttaccgg aggcaaaccg gacgtctttc aactggtgta ccggcgcatt ggcaaagtgg
     1561 gctgctactc cctgcacctg cggtgtgttg atggtgcaga acccctgctg cgtattccac
     1621 acgagttgcg ccgtgctgga ctgcacgccc tgtggcgtaa cccatggcgc gaggtcagct
     1681 gcacggctgg gcgcattccc ttccttgccc aggctcacca gcgcaggccc gaccaggaac
     1741 gcactgccgg gcagcccgcc tttacgttcg gcatttgccg cgcggtcccc gctgtcgcgg
     1801 ttgggatcaa aactggtgga ctctgacagc agcggtggct tgcgctgcca caggtcttgc
     1861 agactgcggt tttcctgcaa taccggagtg cccttggcga tgtagcccat gcggtaggcc
     1921 agcgcggcgg cggggaagct gcccagaatc tcgggcgtgg caaagatcca tctgccctgt
     1981 gagttgggca ggtagccgtt ggccgactgc ggcggcgtcc agccttcgtc accggtggag
     2041 aaccagtagt agccatccac gccggtgagc gatgcgtagg ccgcaatcag gaagggcccc
     2101 tcggcgccaa agttgttggg catcacccag ttgccctcgg tcagcagcat cggacggccc
     2161 tgcgtctgct tcaggtggat gggcagcagc tggggcgtgc gcagaatgct ctcgttggtg
     2221 aactggtcgc ctgccaggat ggcccagccg tttttgggcc ctttgtggat gccaccgttg
     2281 tacacattgg cggcgtctac atcggctgcg gtgtagctcc agcgctcggc atcgttgagc
     2341 agttcggtgc tgccggtctt ccagttgccg gcgttgatga gttgtttggc acccagctcg
     2401 tcgcgcagaa agcggaccat ggtggcattg aagtcgtgca tggtgcgggc cagaaattcg
     2461 gtctggtcag cgcgccgtac cgccttgccg ccgcgcaagt ctttggggtc acccaccagc
     2521 ggccagtcac gtgatggctg ggtcagctcc cagaggttga gcagagtcat gcggccgtcc
     2581 tgtggcgcat ctccggtctc ctgtgcgccc gcccaggcgt cctgcacctt ggccagtgtg
     2641 ccgtatttgc gtgccagaaa cgtggcaaac agccgctcca gcgcctgccg ctgcgcgccc
     2701 tggatgtggt tgaacgacca gaacaacaga ctgtcttcat tctgcagctg caggatggcc
     2761 acgctcgggt cgttgaccag cgcgacgccg gtgtacgggt ttttctcagt cagcagcttg
     2821 cgcagccagg ccttgtaggc gtgttgcagc atcgggtcaa aaaacagcag accagtggcc
     2881 gactgctcgg acccgccggg gaagttccag cctgcggcga acttcatggg gacggcccaa
     2941 tagggcgaga gtgtggtgta aatgccctcc ttgcgcatgg ccgccacgga gcgccagatg
     3001 gcatcgcgct gggcctcatt gatgtcggtg atggcagcgg atggtttggc cttcaggtcc
     3061 ggactgatct gctggtgcag
//
LOCUS       DAOSJM010000300         3076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27650_length_3076_cov_4.400861, whole genome shotgun sequence.
ACCESSION   DAOSJM010000300 DAOSJM010000000
VERSION     DAOSJM010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27650_length_3076_cov_4.400861"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PKX82_10225"
     CDS             complement(<1..465)
                     /locus_tag="PKX82_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOF52674.1"
                     /translation="MADLLVQDALLPAPADQLLQADPAQWPVAPGWKPLVDGFFASAK
                     GVQLVDFLRQRLQAGAVVFPPEPLRALHLTPPEAVRVVILGQDPYHGRGQAEGLAFSV
                     APGVALPPSLRNIFKEIQRDLGELFPEFPQPGGSLVKWARHGVLLLNTCLTVE"
     gene            complement(470..1258)
                     /gene="trpC"
                     /locus_tag="PKX82_10230"
     CDS             complement(470..1258)
                     /gene="trpC"
                     /locus_tag="PKX82_10230"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOF52675.1"
                     /translation="MSDILEKIVAVKHQEVAAAKKRKSLDIVRADAESRVLTRDFVGA
                     LRAKIAAGQPAVIAEVKKASPSKGVIRADFIPADIAQSYAEHGAACLSVLTDRQFFQG
                     EVDYLKQARASCQLPVLRKDFMVDPYQIYESRAMGADCILLIAACLGDAQMQELEAIA
                     LGLDMAVLVEVHDGVELERALKLKTPLVGINNRNLKTFEVSLDTTLTLKAQVPSDRLL
                     VTESGIHTREDVLRMGAAGVNAFLVGEAFMRAPEPGEALEALFG"
     gene            complement(1266..2297)
                     /gene="trpD"
                     /locus_tag="PKX82_10235"
     CDS             complement(1266..2297)
                     /gene="trpD"
                     /locus_tag="PKX82_10235"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOF52676.1"
                     /translation="MPITPQEALQRTIEHREIFHDEMLHLMRQIMSGEMSPVMMAALI
                     TGLRVKKETIGEITAAAQVMREFSTKVDVADKTHLVDIVGTGGDGSHTFNISTCSMFV
                     AAAAGAKVSKHGGRSVSSKSGSADVLEALGLNINLSPAAIAQCIAEQGVGFMFAPNHH
                     PAMKNVAPVRRELGIKTIFNILGPLTNPASAPNILMGVFHADLVGIQVRALQRLGAEH
                     ALVVYGRDGMDEVSLGAATLVGELKNGEITEYEIHPEDFGMVMASNRALKVQTAEDSR
                     AMLLGVLNNQPGAAKDIVVLNAGAALYAADVADSMQAGIQLARAAIESGAAKAKLDAL
                     VALSARLAK"
     gene            complement(2309..2968)
                     /locus_tag="PKX82_10240"
     CDS             complement(2309..2968)
                     /locus_tag="PKX82_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOF52677.1"
                     /translation="MPLDQLLLFIAAGLLLNLTPGPDVLYVVSNALRGGARVGVVAAL
                     GITAGCFVHIFAAAVGVSALMAASATAFTVLKWLGATYLVYVGVRLLLSKAESAIQLE
                     AASAISTGATGQNGLKTIFLRGFWTNALNPKVALFFLAFLPQFITPAMDNKPLAFLLL
                     GLLFNFNGMWVNMGWALTAAWLARRVDAVQRGMHWLDRIAGAMFITFGLKLALTDNPT
                     S"
BASE COUNT          613 a          984 c          909 g          570 t
ORIGIN      
        1 ctccaccgtc aggcaggtat tcagaagaag cactccgtgc cgcgcccact tcaccaggct
       61 tccgccaggc tggggaaatt ccgggaatag ctcacccaga tcgcgctgga tttccttgaa
      121 gatattgcgc aatgatggcg gcaaagccac acctggcgcc accgagaaag caagaccctc
      181 ggcctggccc cgcccgtggt aggggtcctg tcccagaatc acgacccgga cggcttcggg
      241 cggcgtcaga tgcagcgccc gcaagggttc tggcggaaat acgacagcac ccgcttgcag
      301 ccgctggcgc aagaaatcaa ccaactgcac gcccttggcc gaagcaaaga agccgtccac
      361 caggggcttc cagccgggag ccaccggcca ctgggccggg tccgcctgca acagctggtc
      421 cgcaggcgct ggcaatagcg cgtcctgcac caagaggtcc gccatggctt tatccgaaca
      481 aggcttcgag cgcttcgccc ggttcaggcg cccgcatgaa ggcctccccc accaggaatg
      541 cgttgacccc ggcagcgccc atgcgcagca catcctcacg ggtatggatg ccgctctcgg
      601 tcaccagcaa gcggtcggat ggcacctgcg ccttgagcgt caacgtcgta tccagcgaca
      661 cctcgaaggt cttgaggttg cggttgttga tgccgaccag aggcgttttc agcttgagcg
      721 cacgctccag ctcgacgcca tcgtggactt caaccaacac cgccatatcc agacccagcg
      781 cgatggcttc cagctcctgc atctgcgcat ctcccagaca ggcggcgatc agcagaatgc
      841 agtccgctcc catggcacgg gattcgtaga tctggtacgg atcgaccatg aagtccttgc
      901 gcagcaccgg caactggcac gacgcacgcg cctgcttgag gtagtccacc tcgccctgaa
      961 agaactgcct gtcggtcagt accgacagac acgcagcacc atgctcggca tagctctgcg
     1021 caatatctgc cggaatgaaa tccgcgcgga tcacgccctt ggagggactg gcctttttga
     1081 cctccgcgat cacggcgggc tggccggcgg caatcttcgc gcgcaaggca cccacaaagt
     1141 cgcgggtcag cacgcgtgac tcggcgtcgg cacgcacgat gtccagagac ttgcgttttt
     1201 tggccgcagc cacctcctgg tgtttgacgg ctacgatttt ttcgagaatg tcgctcatgg
     1261 tgttcctatt tcgccagccg ggccgagagg gccaccagcg catcgagttt agccttggct
     1321 gctcctgatt caatagctgc tctcgcaagc tggatgccgg cttgcatgct atcggctaca
     1381 tcagcggcat atagcgcggc acctgcgttg agcaccacga tgtccttggc ggcacccggc
     1441 tggttgttca gcacacccag cagcatcgcc ctggaatctt ctgcggtctg cactttgagc
     1501 gcgcggttgc tggccatgac catgccaaag tcttcggggt ggatttcgta ttcggtgatt
     1561 tcgccgttct tcagctcgcc caccagggtc gccgcgccga gggacacttc gtccatgccg
     1621 tcacggccat aaaccaccag cgcatgttcg gcgccaagcc gctgcagcgc ccgcacctgg
     1681 attcccacca gatcggcatg gaacacgccc atcaggatat tgggcgcact ggccggattg
     1741 gtcagcggac ccaggatgtt gaagatggtc ttgatgccga gttcacggcg caccggcgcc
     1801 acattcttca tggccgggtg atggttaggc gcaaacatga agcccacacc ctgctctgcg
     1861 atgcactgcg caatggcagc ggggctgagg ttgatgttga gccccaaggc ttcgagcaca
     1921 tcggcactgc cgctcttgct gctgacgctg cgcccgccgt gcttgctgac cttggcaccg
     1981 gcagcggcag cgacgaacat ggagcaggtc gagatattga aggtgtgcga gccatcgccg
     2041 cccgtgccca cgatgtccac cagatgcgtc ttgtcggcca cgtccacttt ggtggaaaac
     2101 tcacgcatca cctgcgccgc ggcggtgatt tcgccaatcg tctctttctt gacgcgcagg
     2161 ccggtgatca gcgccgccat catgacgggg gacatttcgc cggacatgat ttgccgcatc
     2221 aggtgcagca tctcgtcgtg gaagatttcg cggtgttcaa tggtgcgctg cagcgcttcc
     2281 tgtggggtaa taggcatatc ggatttcctc aagaagtggg attgtcggtc agggccagct
     2341 tcaggccaaa ggtgatgaac atggcgccgg cgatgcggtc gagccagtgc atgccacgct
     2401 gcaccgcgtc gacgcgtctt gccagccatg ctgctgtcag cgcccagccc atattcaccc
     2461 acataccgtt gaagttgaac agcagcccca gcagcaggaa cgccagcggt ttgttgtcca
     2521 tggccggcgt aatgaactgc ggcaaaaagg ccagaaagaa cagcgccacc ttcgggttga
     2581 gcgcattggt ccagaaaccg cgtagaaaga tggttttcaa gccattttgg cctgtagccc
     2641 cggtagatat tgcggatgca gcttccaatt gaatagcaga ctcagccttg gacagcagca
     2701 aacgtacacc cacatagacc aggtaggtag cgcccaacca cttcagcacg gtaaaggcag
     2761 tggcagatgc cgccatcagg gcgctcacac ccaccgcagc agcaaagatg tgcacaaagc
     2821 agcccgccgt gatgcccagc gcagccacca cgcccacccg cgcaccgccg cgcaaggcgt
     2881 tggacactac gtacagcacg tccggccctg gtgtcaggtt cagcagcagg ccagcggcga
     2941 tgaaaagcag cagttggtcc aggggcatgg tggttcctta atacacgccc acgccagagg
     3001 tcgatgcagt ttgtccaggc ggctgctcct ggaagaagcg gcggatgcag tgcagggtgt
     3061 ggtctatccc ggccgc
//
LOCUS       DAOSJM010000301         3075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27661_length_3075_cov_4.454305, whole genome shotgun sequence.
ACCESSION   DAOSJM010000301 DAOSJM010000000
VERSION     DAOSJM010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27661_length_3075_cov_4.454305"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..1110)
                     /gene="hpnC"
                     /locus_tag="PKX82_10245"
     CDS             complement(238..1110)
                     /gene="hpnC"
                     /locus_tag="PKX82_10245"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOF52678.1"
                     /translation="MPAPSTVPSAHQPIDHYENFPVASWLCPKPLRAPITAIYHFART
                     ADDIADEGEATPAQRLAELQAYRQALDAAASGAGPASASHRWPHVFGPLQHAIATHHL
                     PVQLLHDLLDAFVQDIEKTRDHAGYATEAELLDYCRRSANPVGRLLLHLYGVTSEQAL
                     LESDAICTALQLINFWQDLSVDIPRGRYYLSDEMRALHGVDQATVLAREQTEDATKLI
                     AACAYSARASMQKGSELVHHIPGRAGWELRLVVQGGLRILDKIEALHYATLLQRPTLQ
                     WWDFPVMLWRAIWM"
     gene            1259..2455
                     /locus_tag="PKX82_10250"
     CDS             1259..2455
                     /locus_tag="PKX82_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOF52679.1"
                     /translation="MFTRTLIPVATGLAVALALSACSKPAPTEEPIRAVKVMTVGADN
                     IRSGSEYAGEVRARVESRVGFRVGGKIIKRQAELGQRVKAGDVLAQLDPRDYQLSADA
                     ARAGLAAATTNRDLAAADLKRYRDLRDQNFISGAELERREANLKAAQAQLDQAQAQLS
                     GQGNQATYTRLVADVSGVVTSVDAEVGQVVAAGTPVVRIAQDGPRDVVFAVPEDKVAL
                     IRTGSEAEVRGWAAGASTRGVVREVAASADPVTRTFAVKVALGDKDVLPLGATVSVVP
                     QALDRSGVQVIKLPTSAFRQDGKSSAVWVLDTASMTVKNQPVVIATADGNDVVVASGL
                     TLGMQVVIAGVHVLSPGQKVTIYKDKQALAPASNASSATNSIASSAAALAASAPAVAA
                     SQPAAK"
     gene            2463..>3075
                     /locus_tag="PKX82_10255"
     CDS             2463..>3075
                     /locus_tag="PKX82_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOF52680.1"
                     /translation="MSDQHLHPEKGFNLSRWAVEHAPLTKFLMIVLLLLGVTSYFQLG
                     QDEDPPFVFRLMVVRAFWPGATAQQMAEQVTDKIERTLQEVPNADKIRSYSKPGETTI
                     IFQLKDSTRGADVPNTWYTVRKKVGDMRLTLPGGVQGPFFNDEFGDVYGVIYALQADG
                     FSDAEVKTFADDVRQKLLKVADVNKVELFGVQDEKLYIEISQKR"
BASE COUNT          585 a          880 c         1024 g          586 t
ORIGIN      
        1 tttgctccgt gtcccccggc ctttggcctc ctcctttacc tgcgcaaaac gcacagcccg
       61 acgcaaactg cgagcctgtc ttgtgcgcag gctggaatac ctaaaaccca gcttcaggta
      121 gggagtgggt attctgcgga ggtaaaggag gaggccacgc agtggccggg ggacacgtag
      181 cagagtaccc actccctgcc tgttgcgggc gaacgaagaa caactgtgca gggagttcta
      241 catccagata gcacgccaga gcatcaccgg aaaatcccac cactgcagcg tagggcgctg
      301 gagcagcgtg gcgtagtgga gggcctcgat cttgtccaga atccgcagac caccttgtac
      361 caccaggcgc agctcccaac ctgcccgacc ggggatatgg tgcaccagtt cagagccttt
      421 ttgcatgcta gcccttgcgg aatatgcgca agcagctatc aatttggtag cgtcttcggt
      481 ttgctcccgg gccagcactg tggcctggtc cacaccgtgg agcgcacgca tctcgtcact
      541 caggtagtaa cgaccgcgcg gaatgtccac gctcaggtcc tgccagaagt tgatgagctg
      601 cagggcggtg cagatggcgt cgctttccag taacgcctgc tcgctggtga ccccatagag
      661 gtgcagcagc agtcgcccga ccggattggc cgagcggcgg cagtagtcca gcagttcggc
      721 ctcggtggcg tagccagcgt ggtcgcgggt tttctcgatg tcctgcacaa aagcgtccag
      781 caggtcgtgc agcaattgca ccggcagatg gtgcgttgca atggcatgct gcaatggccc
      841 aaaaacgtgg ggccagcggt ggctggctga ggctggcccg gccccggaag cagcagcgtc
      901 cagcgcctgg cggtaggcct gcagttcggc cagacgctgg gcgggggtgg cttcgccttc
      961 gtccgcaatg tcgtcggcgg tacgggcaaa gtggtagatc gcggtaatcg gcgcgcgcag
     1021 gggcttgggg cacagccacg aagctaccgg gaagttttcg tagtggtcga tgggctggtg
     1081 agcggacggg acagtggagg gcgctggcat gtggggattt tgcctgtggc cgggcgggct
     1141 ttcgaaaagt cacttgacaa ctatcaacga aatctctaaa ttactaacca atcggtcagt
     1201 aactccgctg ttactggctg acttccacct tctcccttta ctcccgacgg gcattgccat
     1261 gttcacacgt actctcattc ctgttgcaac gggccttgcc gttgcgcttg cactctctgc
     1321 ctgctccaaa cctgccccaa ctgaagaacc cattcgtgcg gtgaaagtga tgaccgtggg
     1381 agccgacaat atccgctccg gttcggaata cgccggggaa gtgcgtgccc gtgtggagtc
     1441 ccgagtgggc tttcgggtgg ggggcaagat catcaagcgc caggccgaac tcggccagcg
     1501 cgtgaaggct ggtgatgtgc tggcgcagct ggaccccagg gattaccagc tctccgcgga
     1561 tgccgcacgt gccggtctgg ccgcagccac gaccaaccgc gacctggcgg cggcggacct
     1621 gaagcgctac cgtgacctgc gcgaccagaa cttcatcagt ggtgccgagc tggagcgccg
     1681 cgaagccaac ctcaaagccg cccaggccca gctggaccag gcgcaggcgc agttgagcgg
     1741 gcagggcaac caggctacct acacacgtct ggtggcagat gtatctggtg tggtgacttc
     1801 ggtggatgcg gaagtgggcc aggtagtggc cgcaggtacc ccggtggtgc gcattgccca
     1861 ggacggaccg cgggatgtgg tgtttgcagt gccagaagac aaggtggccc tgatccgcac
     1921 gggcagcgaa gccgaagttc gcggctgggc tgccggggcc agcacccggg gcgtggtgcg
     1981 tgaagtggcg gccagtgccg atccggtgac gcgcacgttc gcggtcaagg ttgcgctggg
     2041 tgacaaggat gtgttgcccc tgggcgccac cgtgtcggtg gtgccgcagg cgctggaccg
     2101 cagtggcgtg caggtgatca agttgccgac cagtgccttc cgccaggatg gcaagagttc
     2161 tgcggtgtgg gtgctggata ccgccagcat gacggtcaag aaccagccgg tggtgattgc
     2221 aacggcggac ggcaacgacg tggtcgtggc ctcgggcctg acgcttggca tgcaggtggt
     2281 gattgccggg gtgcatgtgc tctcgcccgg acagaaggtg acaatttaca aggataaaca
     2341 ggccttggcc cccgccagta atgcgtcttc agctactaat tcgatagcat cttccgctgc
     2401 ggcacttgcc gcatcggcac cggcagtcgc tgccagccaa cccgctgcga agtgagatag
     2461 cgatgagcga ccaacatttg caccctgaga agggtttcaa tctgtcccgc tgggcggtgg
     2521 agcacgcgcc gctgaccaag ttcctgatga tcgtgctgtt gttgctgggg gtgacgtcgt
     2581 acttccagct agggcaggac gaagatccgc ctttcgtgtt ccggctgatg gtggtgcggg
     2641 ccttctggcc gggcgccacg gcgcagcaga tggcagagca ggtgaccgac aagatcgagc
     2701 gcacgctgca ggaagtgccc aatgccgaca agattcgcag ctactccaag ccgggcgaaa
     2761 ccaccatcat tttccagctg aaggactcca cacgcggcgc ggatgtaccc aacacctggt
     2821 acaccgtgcg caagaaggtc ggcgatatgc gtctgacgct gcccggtggc gtgcaggggc
     2881 cttttttcaa tgacgagttt ggtgatgtgt atggcgtgat ttacgccctg caggccgatg
     2941 gtttctccga tgcggaagtc aagacttttg ccgacgatgt gcgccagaag ctgctgaagg
     3001 tggcggatgt gaataaagtg gaattgttcg gtgtgcagga cgagaagctg tacatcgaaa
     3061 tttcgcagaa acgcc
//
LOCUS       DAOSJM010000302         3058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_27856_length_3058_cov_3.790210, whole genome shotgun sequence.
ACCESSION   DAOSJM010000302 DAOSJM010000000
VERSION     DAOSJM010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27856_length_3058_cov_3.790210"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKX82_10260"
     CDS             complement(<1..1006)
                     /locus_tag="PKX82_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOF52681.1"
                     /translation="MGDTTHNNNTAAQDFIARWSGKAASELSTAQSFVNELCALLGVV
                     PPTHELDYMFERPITFRHGDGSSSAGRIDCYRRGHFVLEAKKLKAGPATKGFDDALLR
                     ARAQAEGYARALPASEGRPPFCVVVDVGNVIELYSEFSRSGATYTPYPDPRSHRIKLA
                     DLARPEVQATLRAIWTDPTSLDPARASARVTREVAAQLAGLAKSLEQSGHRADHVGAF
                     LTRCLFSMFAEDVELLPKGSFLNLLQTWREQPATLQQMLRTLWADMDHGGFSAALAAP
                     VMHFNGKLFKEPASDGYSLLLNQEQIDLLITAARANWREVEPAIFGTLLERALDPTER
                     H"
     gene            complement(1028..1378)
                     /locus_tag="PKX82_10265"
     CDS             complement(1028..1378)
                     /locus_tag="PKX82_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HOF52682.1"
                     /translation="MLYYALKVFISALLIVGISEIAKRSSGLAALLAALPLTSLLAFV
                     WLHLEATPPRDIADLSIQIFWLVIPSLLLFLLLPLMLRHGWNFWLSLALSMAATAGFY
                     LTLLPLLRRMGVQL"
     gene            1451..1951
                     /locus_tag="PKX82_10270"
     CDS             1451..1951
                     /locus_tag="PKX82_10270"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOF52683.1"
                     /translation="MTSTPSPTDFDDISPASNIAIPDGFRPVQVGGAFIAHNGPLYGK
                     WTGERLLLGFRVQDQHTNPLKIAHGGMMATFADMLIPCAAMYQHAMERRFLPTISLQM
                     DYMGASPLGAWVQGEADVLKTTRNMLFGQGVVTADGQPVLRVSGIFKMGQLIGDGTAT
                     DPLGLK"
     gene            2021..2431
                     /locus_tag="PKX82_10275"
     CDS             2021..2431
                     /locus_tag="PKX82_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOF52684.1"
                     /translation="MAINIQWDNKFEVGHERIDFEHRIFLGLIRDLSLEAERNPQSPR
                     VTRMLLEIHKYAEFHFLSEENIMLDVGYPHLVEHQRVHKRILNQLGDKIHQYQHQGIH
                     DSAEDIVTFMFEWFALHTTSEDKRIAEFIRATTT"
     gene            2428..>3058
                     /locus_tag="PKX82_10280"
     CDS             2428..>3058
                     /locus_tag="PKX82_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF52685.1"
                     /translation="MTTTTLNPSSSVPLKQDATIIGLVGLAHASSHFGHLLLPLMFPV
                     FMKEFGLSYSELGALMSVFFVVSGVGQASAGFVVDRLGARPLLFVALGIFAAACVAAS
                     TVTGYTGLMLVAALAGLGNATFHPVDFTILNQRVSGPRLGYAFSAHGLTGNLGWAVAP
                     VFFASISAGFGWRTAYLAAAVLYAAILALLVVQRDKLSTQVVVHEPHVHK"
BASE COUNT          549 a          888 c         1039 g          582 t
ORIGIN      
        1 cgtggcgctc ggtggggtcg agcgcgcgtt ccagcagggt gccgaagatg gcgggttcca
       61 cctcgcgcca gttggcgcgt gcggcggtga tgagcaggtc gatctgctcc tgattcaata
      121 gcaggctata cccgtctgac gcgggctcct tgaacaattt gccattgaag tgcatgaccg
      181 gcgcggccag cgcggcgctg aagccgccgt ggtccatgtc ggcccacagg gtgcgcagca
      241 tctgctgcag cgtggcgggt tgttcgcgcc aggtttgcag caggttcagg aagctgcctt
      301 tgggcagcag ctccacgtct tcggcaaaca tgctgaacag gcagcgggtc aggaaggcac
      361 cgacgtggtc ggcacggtgg ccgctctgtt cgagggactt ggccaggcct gcgagttgcg
      421 cggccacttc gcgggtgacg cgggcgctgg cgcgtgccgg gtccagactg gtggggtcgg
      481 tccagatggc gcgcagggtg gcctgcacct cgggccgtgc caggtcggcc agcttgatgc
      541 ggtggctgcg cgggtcgggg taaggtgtgt aggtggcgcc gctgcggctg aactcggaat
      601 acagctcgat gacgttgccg acatccacca ccacgcagaa gggcgggcgg ccctcggagg
      661 cgggcagggc gcgggcatag ccctcggcct gggcacgggc gcgtaagagc gcgtcgtcga
      721 agcctttggt ggcggggcct gccttgagct ttttggcttc cagcacgaag tggccacgcc
      781 ggtagcagtc gatacgcccg gcgctgctgg agccgtcgcc atggcggaag gtgatggggc
      841 gctcgaacat atagtccagc tcgtgcgtgg gtggtaccac gcccagcagg gcgcacaact
      901 cgttgacaaa gctttgcgcg gtggatagct cggaggcggc tttgccgctc cagcgcgcaa
      961 tgaagtcctg cgccgctgta ttgttgttat gtgtggtgtc tcccatgcgg gggatgatag
     1021 cgggacctta caactgcacg cccatgcgcc gcagcagggg cagcaaggtg aggtagaagc
     1081 ctgcggtggc cgccatcgac agggccaggc tcagccagaa attccagccg tggcgcagca
     1141 tgagcggcag cagcagaaac agcagcagcg atggaatgac cagccagaag atctggatgg
     1201 agaggtcggc aatatccctg ggcggcgtgg cttcgaggtg cagccagaca aacgccagca
     1261 gcgaggtgag cggcagggcg gccagcagcg cagccaggcc gctggagcgc ttggcgattt
     1321 ccgagattcc gactatgagc agtgcggaaa tgaagacctt gagagcgtag tacagcatgg
     1381 ggcgcatggt accaagccgg cctgcaaact gcgtgacagc agttcctgca tccgtggata
     1441 agatgccagc atgacctcca caccatcccc caccgacttc gacgacatct cgcccgccag
     1501 caatattgcg attccggacg gtttccgccc ggtgcaggtg ggcggcgcgt ttatcgccca
     1561 caacggcccg ctgtatggca aatggaccgg cgagcgcctg ctgctggggt ttcgcgtgca
     1621 ggaccagcac accaatccac tcaagatcgc gcacggcggc atgatggcca cctttgccga
     1681 catgctgatt ccctgtgccg ctatgtacca gcacgcaatg gaacgccgtt ttttgccgac
     1741 catcagcctg cagatggact acatgggcgc atcgcctctg ggtgcctggg tgcagggcga
     1801 ggccgatgtg ctcaagacca cgcgcaacat gctgtttggc cagggcgtgg tgacggccga
     1861 tggccagccg gtgctgcgcg tgagtggcat tttcaaaatg gggcagctca ttggtgacgg
     1921 cacggcaacc gatcctctgg ggttgaagta agtcgggtgg gctgctggcc agcctgccta
     1981 gaatggggca ctacaaacca caagacaggg cgggcatcgg atggcgatca atatccagtg
     2041 ggacaacaag tttgaggtcg ggcacgagcg cattgacttc gagcaccgga tttttctggg
     2101 cctgattcgc gacctgtccc tggaggcaga acgcaatccg caaagccccc gcgtgacgcg
     2161 gatgcttctg gaaattcaca agtacgccga gttccacttc ctgagcgaag agaacatcat
     2221 gctggatgtg ggctacccgc acctggtcga acaccagcgc gtgcacaagc gcattctcaa
     2281 ccagctgggc gacaagattc accagtacca gcaccaggga attcatgact cggccgaaga
     2341 catcgtgact tttatgttcg agtggtttgc gctgcacacc acctctgaag ataagcgcat
     2401 cgccgagttc atccgcgcca ccacaacatg actactacga ccctgaatcc ctccagcagc
     2461 gttccactca aacaggatgc caccatcatc ggtctggtgg gcttggccca tgccagttcg
     2521 cacttcgggc acctgctgtt gccgctgatg tttccggtgt tcatgaagga gtttggcctg
     2581 agctactcgg agctgggagc gctgatgagc gtcttctttg tggtttctgg cgtggggcag
     2641 gccagtgcgg gttttgtggt ggaccgcctg ggcgcgcggc cgctgctttt tgtagcgctg
     2701 ggcatttttg cggcggcctg cgtggccgcg tcgaccgtga ccggctacac cgggttgatg
     2761 ctggtagccg cattggcggg attgggcaat gcgacttttc accccgtgga cttcaccatc
     2821 ctgaaccagc gtgtctccgg gccacgcctg ggctatgcct tcagcgcgca tggattgacg
     2881 ggcaacttgg gctgggcggt ggcgccggtg ttttttgcgt ccatcagcgc gggctttggc
     2941 tggcgcaccg cctatctggc agcggccgtg ttgtacgcgg cgatcctggc gctgctggtg
     3001 gtacagcgcg acaagctcag tacacaggtg gtggtgcatg aaccccatgt gcacaagg
//
LOCUS       DAOSJM010000303         3047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28011_length_3047_cov_4.052139, whole genome shotgun sequence.
ACCESSION   DAOSJM010000303 DAOSJM010000000
VERSION     DAOSJM010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28011_length_3047_cov_4.052139"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PKX82_10285"
     CDS             complement(<1..826)
                     /locus_tag="PKX82_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOF52686.1"
                     /translation="MSTKQIRNIAIIAHVDHGKTTMVDQLLRQSGTFAEHEKVVDTVM
                     DNNAIEKERGITILAKNCAVSWEGTHINIVDTPGHADFGGEVERALSMVDSVLLLIDA
                     QEGPMPQTRFVTKKALALGLKPIVVVNKVDKPGANPDKVVNAAFDLFDKLGATDEQLD
                     FPVVYASGINGWSSLEEGEPGEQWGPDMSALFNTILKHVPPNSGDPKAPLQLQISALD
                     FSTFVGRIGVGRISQGTLKPMMDVVVMEGPDGKAVKGRVNQVLTFQGLDRMQTQEAG"
     gene            complement(823..1800)
                     /gene="truB"
                     /locus_tag="PKX82_10290"
     CDS             complement(823..1800)
                     /gene="truB"
                     /locus_tag="PKX82_10290"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOF52687.1"
                     /translation="MNAPRARVARRPVHGVLLLDKPIGLSSNQALQKAKWLLRAEKAG
                     HTGTLDPLATGVLPLCFGAATKFSQMHLDADKTYLATARLGLKTSTGDAEGEVISERP
                     VQVTQAQLTDVVQGLTGPIRQVPPMHSALKKDGKALYEYARAGIEVERAARDVTIYEL
                     KVTQALVDSAQPAIEFIAKCSKGTYIRTLGEDMGEALGCGAHLTALRRIRTGDFDASQ
                     CITLEALEAMDENARLARLMPVESLLTGHTVVTLDSENAARFLSGMRRRGPWADAQEV
                     VVYASGPRALLGTAHVKAGELIPGRLLSPVEISEVQAQNELLKLAESEL"
     gene            complement(1805..2176)
                     /gene="rbfA"
                     /locus_tag="PKX82_10295"
     CDS             complement(1805..2176)
                     /gene="rbfA"
                     /locus_tag="PKX82_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOF52688.1"
                     /translation="MVRKKSSTPNRGFRVADQIQRDLTELIARELKDPRVGMVTIQAV
                     EVTPDYAHAKVFFSLLVGDPVETAEGLNQAAGFLRAGLFKRLHIHTVPTLHFIFDQTT
                     ERAADMNALIARAVASRAKED"
     gene            complement(2189..>3047)
                     /locus_tag="PKX82_10300"
     CDS             complement(2189..>3047)
                     /locus_tag="PKX82_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOF52689.1"
                     /translation="RVRAMLDENGKSIKTAGPSIPVEIQGLTEVPQAGDEFMVMSDER
                     RAREIATYRAGKFRNTKLAKQQAAKLENMFTDMTAGDVKMVPIIVKADVQGSQEALAS
                     SLLKLSTDEVKVQMVYTAVGGISESDVNLAIASKAIIIGFNTRADAGARKLAENNGVD
                     IRYYNIIYDAVDELKAAMSGMLAPEKREEVIGFAEIRTVFVASKIGTVAGCMVTGGFV
                     NRTAHFRLLRDNVVIYTGELDSLKRMKDDVREVKEGFECGIKLKNYNDIKEGDQLEFF
                     EVKEIARTL"
BASE COUNT          574 a          966 c          836 g          671 t
ORIGIN      
        1 gacccgcttc ctgggtctgc atacgatcca gaccctggaa agtcagcacc tggttgacac
       61 ggcctttgac cgccttgccg tcagggcctt ccatgacgac cacatccatc atgggtttca
      121 gcgtaccctg gctgatgcgg cccacgccga tgcggcccac aaaggtcgaa aagtccagcg
      181 ccgagatctg cagttgcagc ggggccttgg ggtcgccgga gtttggaggt acgtgcttca
      241 ggatggtgtt gaacagcgcg gacatgtcgg ggccccattg ctcaccgggc tcgccctctt
      301 ccagcgacga ccagccgttg ataccggagg catacaccac ggggaagtcc agctgttcgt
      361 cggtcgcacc cagcttgtcg aacaggtcga acgcggcgtt gaccaccttg tcggggttgg
      421 caccgggctt gtccaccttg ttcaccacca cgatgggctt gagacccagg gccagtgcct
      481 tcttggtcac aaagcgggtc tggggcatcg ggccttcctg tgcatcaatc agcagcaaca
      541 cgctgtccac catactgagc gcacgttcca cctcaccgcc gaagtcggcg tgtccggggg
      601 tgtcaacgat gttgatgtgg gtaccttccc agctcacggc gcagttcttg gccagaatcg
      661 tgatgccacg ctctttttca atcgcgttgt tgtccatcac ggtatcaacc actttttcat
      721 gctcggcaaa ggtgccggac tggcgcagca actggtccac catggtggtt ttgccatggt
      781 ccacgtgggc gatgatggcg atattgcgaa tttgtttggt actcatagtt cactttctgc
      841 taacttcaat aattcgttct gggcttgtac ttcgctgatt tccacgggac tcagaagtcg
      901 ccccggaatc aactcacccg ccttcacatg ggccgtaccc agcaatgccc tggggccgct
      961 ggcatacacc acgacctctt gcgcatcggc ccagggacca cgcctgcgca tgccactgag
     1021 gaaccgggct gcattctcgc tgtccagtgt gacaaccgtg tgccctgtca agagggactc
     1081 cacaggcatc aagcgcgcca gccgcgcatt ttcgtccatt gcttccagcg cttccagcgt
     1141 gatgcactga gatgcgtcaa agtccccagt gcggatcctg cgcaacgccg tcaggtgagc
     1201 cccgcaaccg agcgcctcac ccatgtcctc acccagagtc cgtatatacg ttcccttgct
     1261 gcactttgct ataaattcaa tagctggctg cgcactatcc acgagggctt gcgtcacttt
     1321 tagctcataa atcgtgacat cacgcgccgc ccgctctact tcaatcccgg ccctggcgta
     1381 ctcgtacagg gctttaccgt cctttttcaa ggcactgtgc ataggcggaa cctgccggat
     1441 cggcccggtc aacccctgca ccacatccgt caactgcgcc tgcgttacct gcacaggacg
     1501 ctcactgatc acctcaccct cggcatcgcc ggtgctggtt ttcagaccca accgtgccgt
     1561 ggccaggtag gtcttgtccg catccagatg catctggctg aacttggtcg ctgcaccaaa
     1621 acacaagggc aacaccccgg tcgccaacgg gtccagcgta ccggtgtgcc ctgccttttc
     1681 ggcccgtaaa agccacttgg ccttctgcaa cgcctggttg ctcgacaggc caatcggttt
     1741 atccagcaga agcaccccat gcacagggcg ccttgcaacc cgtgcacgtg gcgcattcat
     1801 ggcgtcaatc ttcctttgcg cgcgaagcca ccgcacgggc gatcaaggca ttcatgtccg
     1861 ccgcgcgctc tgtcgtctgg tcaaaaatga aatgcaaagt gggcacggtg tggatatgca
     1921 ggcgcttgaa taggcctgcg cgcagaaaac ccgcagcctg attcaggcct tccgccgtct
     1981 ccaccggatc accaaccaac aaactgaaga acaccttggc gtgcgcatag tctggcgtga
     2041 cctccactgc ctgaatcgtc accatgccaa cccgcggatc cttgagctcg cgcgcgatca
     2101 gctcggtcag atcgcgctgg atctgatcgg ccacgcggaa gccgcggttg ggggtggatg
     2161 actttttgcg gaccatggtg tgtttggctt acagcgtacg ggcgatttcc ttgacctcga
     2221 agaactccag ctgatcacct tccttgatgt cgttgtagtt cttgagcttg ataccgcact
     2281 cgaagccttc cttgacttcg cgcacatcgt ctttcatgcg cttgagcgaa tccagctcgc
     2341 cggtgtagat caccacgttg tcgcgcagca ggcggaaatg tgccgtccgg ttgacaaaac
     2401 cgcctgtgac catacaaccc gcgaccgtac cgatcttgga tgccacgaac acggtgcgga
     2461 tctcggcaaa gccaatgact tcttcgcgct tctcgggagc cagcatgccg gacatggccg
     2521 ccttcaactc atcgacggcg tcgtaaatga tgttgtagta acggatatcc acgccattgt
     2581 tttcggccag tttgcgggca ccggcatctg cacgggtatt gaacccgatg atgatggcct
     2641 tggaagcaat cgccaggttg acgtccgatt cgctgatacc gcccacagcg gtgtagacca
     2701 tctggacctt gacctcgtcg gtcgacagct tgagcagcga ggaagccagc gcctcctggg
     2761 agccctgcac atcggccttg acgatgatcg gaaccatctt gacgtcgcca gccgtcatgt
     2821 cggtgaacat gttctccagc ttggcggcct gctgtttggc cagcttggtg ttgcggaact
     2881 tgccggcacg gtaggtggca atttcacggg cacggcgttc gtcggacatc accatgaatt
     2941 cgtcgccagc ctgcggtact tccgtcagac cctggatttc caccggaatg gaaggccctg
     3001 cggtcttgat cgatttgccg ttttcgtcca gcatggcgcg cacgcgg
//
LOCUS       DAOSJM010000304         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28078_length_3041_cov_5.346618, whole genome shotgun sequence.
ACCESSION   DAOSJM010000304 DAOSJM010000000
VERSION     DAOSJM010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28078_length_3041_cov_5.346618"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..1885
                     /gene="argS"
                     /locus_tag="PKX82_10305"
     CDS             212..1885
                     /gene="argS"
                     /locus_tag="PKX82_10305"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOF52690.1"
                     /translation="MLTVKTELLQALAACLESLSPGAGSKAAFESPKVAAHGDLATTA
                     AMQLAKPLKQNPRQLAENLRTAMLAQPAFAAWVDAVEIAGPGFINIRLKPSAKQQIVR
                     EVLGQRERFGVQPDKGQRMLVEFVSANPTGPLHVGHGRQAALGDAICNLFQTQGWDVY
                     REFYYNDAGVQISTLANSTQLRAKGFKPGDAEWPEAAYNGDYIADIAGDYLAKKTVHS
                     DDREFTASGDVDALDDIRLFAVAYLRHEQDLDLKAFSVKFDNYYLESSLYTSGSVDAA
                     VQKLRDAGKTYEQDGALWLKSTDYGDDKDRVMRKGDGSYTYFVPDVAYHISKWERGFT
                     KVVNIQGTDHHGTIARVRAGLQAADVGIPHGYPDYVLHTMVRVVKGGEEVKISKRAGS
                     YVTLRDLIEWTSKDAVRFFLLSRKPDTEYTFDVDLAVAKNNDNPVYYVQYAHARICSV
                     LATWGGDVATLQGVDLSALQSPQAQALMLLLAKYPDMLTAAAVDFAPHDVTFYLRELA
                     ASYHSYYDAERILVDDEAVKLARLALVAATAQVLHNGLAVLGVSAPQKM"
     gene            1918..2529
                     /locus_tag="PKX82_10310"
     CDS             1918..2529
                     /locus_tag="PKX82_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOF52691.1"
                     /translation="MKHQRGGTFLGFIIGVVVGLAAALAVAVYVTKVPVPFMNKGQSR
                     SAEQDAAEAKKNKDWDPNAPLYGKNPAKAAQPAVPPEAAASKPAGKDEAKPADKKAEN
                     KPAVSADPLGDLAKAKAAAAEGEPFVYYVQVGAFRTPEDAESHRAKLSLSGIETKVSE
                     REQSGRMVYRVRVGPFEKKEDGERAKEKLDKAGLDTALVRVQR"
     gene            2603..>3041
                     /locus_tag="PKX82_10315"
     CDS             2603..>3041
                     /locus_tag="PKX82_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HOF52692.1"
                     /translation="MKRRDFSRMAASSALLASLAPVMVQAQGKKPVPGTDYQVLDPRA
                     PVEAPAGKIEVVEFFWYNCPHCATLEPTLNEWVKRLPKDVAFRRVPRRFADGEVPQQQ
                     LYYALESMGLVDKLHAKVFAAVHGERRNLNRPEAIADWVAAQGV"
BASE COUNT          601 a          966 c          963 g          511 t
ORIGIN      
        1 ggtgctttgt cgtcggcagc gtgggccacg cccattgcca gtgtcaaacc caaggccacg
       61 agggaaatca gttttttcat gtgtcgtcct taaagaaatg tgacagttgc gtcgcctttc
      121 ggctggaatc cgcacatgga ttccagcaca taacgcactg gctggcaatg cggatgaccc
      181 ccggcgcgcc acccccgtaa aatcagcccc catgctcacc gtcaaaaccg aactcctgca
      241 ggcactggct gcctgtctgg aatccctctc ccccggcgcc ggctccaaag ctgcgtttga
      301 atcccccaaa gtggccgccc atggcgacct ggccaccacg gccgccatgc aattggccaa
      361 gccgctcaag cagaacccgc gccagctggc cgagaacctg cgcaccgcca tgctggccca
      421 gcccgcgttt gcggcctggg tggatgcggt ggagattgcc ggccccggtt ttatcaatat
      481 ccgcctcaag ccctcggcca agcagcagat cgtgcgtgag gtgctgggcc agcgagagcg
      541 cttcggtgtg cagcccgaca agggccagcg catgctggtg gaattcgtgt ccgccaatcc
      601 caccgggccg ctgcatgtgg gccacggccg ccaggcggcg ctgggcgatg cgatctgcaa
      661 cctgttccag acccagggtt gggatgtgta ccgcgagttc tattacaacg acgccggtgt
      721 gcagatcagc accctggcca actccaccca gctacgcgcc aagggcttca agccgggcga
      781 cgccgagtgg cccgaggccg cctacaacgg cgactacatt gccgacatcg ctggtgacta
      841 cctggccaaa aagacggtgc attccgacga ccgcgaattc accgccagcg gcgatgtgga
      901 tgcgctcgac gacatccgcc tgtttgccgt ggcctacctg cgccacgagc aggacctgga
      961 cctcaaggcg ttctcggtca agtttgacaa ctactacctg gagtcgagcc tgtacaccag
     1021 tggcagcgtg gacgccgcgg tgcaaaagct gcgcgatgcc ggcaagactt acgaacagga
     1081 cggcgcgctg tggctcaagt ccaccgatta cggggacgac aaggaccgcg tcatgcgcaa
     1141 gggtgacggc agctacacct actttgtgcc ggatgtggcg taccacatca gcaagtggga
     1201 acgcggtttc accaaggtcg tcaacatcca gggcaccgac caccacggca ccattgcccg
     1261 cgtacgcgcg ggcctgcagg ccgccgatgt cggcatcccg cacggctatc ccgactatgt
     1321 gttgcacacc atggtgcgcg tggtcaaggg gggtgaggaa gtcaagattt ccaagcgtgc
     1381 gggcagctat gtgacgctgc gcgacctgat cgagtggacc agcaaggacg cggtgcgctt
     1441 cttcctgctg agccgcaaac ccgacaccga gtacaccttc gatgtggacc tggcggtggc
     1501 caaaaataac gacaacccgg tgtactacgt gcagtacgcg catgcgcgca tctgctcggt
     1561 gctggccacc tgggggggtg atgtggccac cctgcagggc gtggacctgt cggcgctaca
     1621 gagcccgcag gcgcaggccc tcatgctgct gctggccaag taccctgaca tgctgacggc
     1681 tgcggccgtg gacttcgcac cccacgacgt gaccttctac ctgcgcgagc tcgcggccag
     1741 ttaccacagc tactacgatg cagagcgcat tctggtggac gacgaggccg tcaagctggc
     1801 ccgtctggcc ctggtcgccg ccacggcgca ggtgttgcac aatggcctgg cagtgctggg
     1861 cgtgagtgcc ccgcaaaaaa tgtaatccat gttttcaaca agcagcgaga caaaggcatg
     1921 aagcatcagc gcggcgggac ctttctgggg ttcatcattg gggtggtggt cgggctggcg
     1981 gcggcgctgg ccgtggccgt gtacgtcacc aaggtaccgg tgcctttcat gaacaagggg
     2041 caaagccgca gcgccgaaca ggacgcggcc gaggccaaga agaacaagga ctgggacccc
     2101 aacgcaccgc tgtacggcaa gaatcccgcc aaggcggcgc aacccgccgt accgcctgaa
     2161 gccgccgcta gcaagcccgc aggcaaggat gaggccaagc ccgccgacaa gaaggccgag
     2221 aacaaaccgg ccgtgtcggc ggaccccctg ggcgacctgg ccaaagccaa ggccgctgca
     2281 gccgaaggcg agccttttgt gtactacgtg caggtgggcg ctttccgcac cccggaggac
     2341 gccgagagcc accgtgccaa gctgtccctc agcggtatcg aaaccaaagt gtccgagcgc
     2401 gaacagtcgg gccgcatggt gtatcgcgtg cgggtgggtc ctttcgagaa aaaggaagac
     2461 ggtgagcgtg ccaaggagaa actcgacaag gccgggctgg atacggccct ggtgcgggta
     2521 cagcgctagc cagccaccgc gcccggaact ttctggagcc tctgcggtct gacctgtcca
     2581 atccactgga gatatgtgat ttatgaaacg tcgtgatttt tcccggatgg ctgcatcctc
     2641 ggcgctgttg gcgtctctgg cgcctgtcat ggtccaggcg cagggcaaaa agccggtccc
     2701 cggtaccgat taccaggtac tggacccacg cgcaccggtc gaagcgcctg ccggaaaaat
     2761 cgaggtggtg gagttcttct ggtacaactg cccgcactgc gccacgctgg aacccacgct
     2821 caatgagtgg gtcaagcgtc tgcccaagga tgtggcattc cgccgcgtgc cgcgccggtt
     2881 tgccgacgga gaagtgccgc aacagcagct gtattacgca ctggagtcca tgggcttggt
     2941 ggacaagctg catgccaagg tgtttgccgc tgtgcatggc gagcgccgca atctgaatcg
     3001 ccccgaggcc attgccgact gggtggcggc acagggtgtc g
//
LOCUS       DAOSJM010000305         3041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28088_length_3041_cov_3.399531, whole genome shotgun sequence.
ACCESSION   DAOSJM010000305 DAOSJM010000000
VERSION     DAOSJM010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28088_length_3041_cov_3.399531"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKX82_10320"
     CDS             complement(<1..259)
                     /locus_tag="PKX82_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOF52693.1"
                     /translation="MDNSDDLKAAQKKALRKELLEQRLNLPDRLQRADLLQRVMRIWL
                     VGRPDAVIGAYWPIKGEFDPLPALHRWKEDGELLDEPQPRRI"
     gene            269..2269
                     /locus_tag="PKX82_10325"
     CDS             269..2269
                     /locus_tag="PKX82_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOF52694.1"
                     /translation="MQFSSILTSFALLGALTLSPFTPVLAQGRDNTRVDEAILDMAQA
                     YKQRDRKRLTALLPQVRGYVLEPWGAYWELSARLDDAGPSEIQDFLNRYSGTYQEDRL
                     RADWLLQLGKRRDWQAFNREYPKYRMNDDKSVRCYALLAEHLSGGTDVNKAVYDTWLS
                     QKDADEGCAAAAEQLVKDHSTRKQPVWLRARLGMENDRLRIATQAVGLLNDSWTKTVN
                     AIYLNPGKYLNDKLTALRPQTREFVSLALIRQAYLEPAEAAVEVEKLRWKAQLTQEER
                     SWIWGVIGKRAAMKQSSDAPAYFANGQFAQMHEDHLAWAARANLRAGRWTQVHAAISA
                     MPEAMRKEPTWVYWHARALLAQASNESERAEALRLLEGIASVRGFYEQLALEEIGQRI
                     STPEKPAPLTPEEKEAARQNPGLVRALYAIRIGLRPEGVREWNYTTNLHTRGGMDDRA
                     LLAAADMACAVEVWDRCINTSERTKTVVDLEQRYPMPFRNAVTTRAKQINLDPAYVYG
                     LIRQESRFIMDARSHVGASGLMQVMPATARWTAKKIGLTDFQAHQITDRDTNIAIGTG
                     YLKLVLDNFGGSMPMAAAAYNAGPNRPRTWRGQAGAPVLEAAIWAENVPFNETRDYVK
                     KVLSNTTNYAALITGQPQSLKARLGTVGPRDTAQPEAGLDLP"
     gene            2284..2967
                     /locus_tag="PKX82_10330"
     CDS             2284..2967
                     /locus_tag="PKX82_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOF52695.1"
                     /translation="MLKLLIGNKNYSSWSMRPWVLLKQAGIPFEEVKVRFDSFDASST
                     FKKTLAGVTPTAKVPVLQDGDLAIWDTLAIGEYVAEQFPDKQLWPAERVARARARSIC
                     AEMHSGFTGLRGNCPMNIEAHLPDNGALIWRDKPAVRSDLERIISMWSALLQEHGGPM
                     LFGAFTVADAYFAPVCMRIQTYALPVPPVVQAYIDRVCALPGVKAWIDDARAEQDFLD
                     FEEPYRLQR"
BASE COUNT          592 a         1023 c          925 g          501 t
ORIGIN      
        1 caatgcggcg cggctggggt tcgtcgagca gctcgccatc ttccttccag cggtgcaggg
       61 ctggcaaagg atcgaactcg cccttgatgg gccagtaggc gccaatgacg gcatcggggc
      121 gacccaccag ccagatgcgc atgacacgct gcaaaagatc cgcacgctgt aggcggtccg
      181 gcaggttcag gcgttgttca agcagttcct tgcgcagcgc tttcttctgt gccgccttca
      241 gatcatccga gttgtccata atccccacat gcagttttct tccattctga catcgttcgc
      301 gctcctgggc gccttgacgt tgtccccgtt cactcccgtt ctggcccagg gccgggacaa
      361 cacccgtgtc gacgaggcca ttctggacat ggcccaggct tacaaacagc gcgaccgcaa
      421 gcgtctgacc gccctgctgc cccaggtccg tggctatgtg ctggagccct ggggtgccta
      481 ctgggagctg tcggcccgcc tggacgacgc gggccccagc gagatccagg atttcctgaa
      541 ccgctacagc ggcacatacc aggaagaccg cctgcgcgcc gactggctgt tgcagctggg
      601 caagcgccgc gactggcagg ccttcaaccg cgagtacccc aagtaccgca tgaacgacga
      661 caaatcggtg cgctgctatg cgctgctggc agaacacctc agcggtggca ccgatgtcaa
      721 caaggcggtc tacgacacct ggctctcgca gaaggatgcc gacgaaggct gcgccgctgc
      781 ggccgagcag ctggtcaagg accactccac ccgcaaacag ccggtgtggc tgcgcgcccg
      841 gctgggcatg gaaaacgaca gactgcgcat tgccacccag gcggtcggtc tgctcaatga
      901 cagctggacc aaaaccgtca acgcgattta cctcaatccc ggcaaatacc tcaacgacaa
      961 gctgaccgcc ctgcggccgc agacccgcga gttcgtctcc ctcgcgctga tccgacaggc
     1021 ctacctggag cctgccgagg ccgctgtcga agtggaaaag ctgcgctgga aggcccagct
     1081 gacgcaggaa gagcgcagct ggatctgggg cgtgatcggc aagcgcgctg ccatgaaaca
     1141 gtccagcgat gccccggcgt actttgccaa cggacagttt gcgcagatgc acgaagacca
     1201 cctggcctgg gccgcccgcg ccaacctgcg ggctgggcgc tggacgcagg tgcatgccgc
     1261 catcagcgcc atgcccgagg ccatgcgcaa ggagcctacc tgggtctatt ggcacgcacg
     1321 cgccttgctg gcccaagcca gcaacgagag cgaacgcgcc gaggcgctgc gcctgctcga
     1381 aggcattgcc agtgtgcggg gtttttacga acaactggcg ctggaagaaa tcggtcaacg
     1441 catcagtacg cccgagaagc ccgcgccact gaccccggaa gaaaaagaag ccgcccggca
     1501 gaaccctggc ctggtgcgtg cgctctacgc gattcgcatt ggcctgcggc ctgaaggcgt
     1561 acgcgaatgg aactacacca ccaacctgca cacccgcggc ggcatggacg accgcgcgct
     1621 gctggcggcg gctgacatgg cctgtgcggt ggaagtgtgg gaccgctgca tcaacaccag
     1681 cgagcgcacc aagaccgtgg tggacctgga gcagcgctat cccatgccgt ttcgcaatgc
     1741 cgtcaccacc cgggccaagc agatcaacct ggatccagcc tatgtgtatg gcctgatccg
     1801 ccaggaaagc cgcttcatca tggacgcccg ctcgcatgtg ggtgcgtccg gactgatgca
     1861 ggtgatgcca gccaccgcgc gatggaccgc caaaaagatc ggtctgaccg acttccaggc
     1921 gcaccagatc accgaccgtg acaccaatat tgccattggc accggctacc tcaaactggt
     1981 gctggacaac tttggcggct ccatgcccat ggccgctgcc gcctacaacg ccggccccaa
     2041 ccggccccgt acctggcgcg gacaggccgg tgcaccagtg ctggaggccg ccatctgggc
     2101 cgagaatgtg ccgttcaacg agacccgcga ctacgtcaag aaagtgctgt ccaacaccac
     2161 caactacgcc gccctcatca ccggccagcc ccagtcgctg aaagcccgcc tgggcaccgt
     2221 ggggccgcgt gacaccgcgc agcccgaggc cgggctggac ttgccctaac acctggagat
     2281 cacatgctca aactcctcat tggtaacaag aactactcgt cctggtcgat gcggccatgg
     2341 gtgctgctga agcaggcggg tatcccgttt gaggaagtca aggtgcgctt tgattccttc
     2401 gatgccagct ctaccttcaa gaaaaccctg gctggcgtca cccccacagc caaggtgcca
     2461 gtgctacagg acggcgatct ggccatctgg gacaccctgg cgatcggcga atacgtagcc
     2521 gagcagtttc ctgacaagca gctctggccc gcagagcgtg tggcccgggc acgcgcgcgc
     2581 agcatctgcg ccgaaatgca cagcggcttt accggcctgc gcggcaactg cccgatgaac
     2641 atcgaagccc acctgcccga caacggcgca ctgatctggc gcgacaagcc cgcagtacgc
     2701 agcgatctgg agcgcatcat ttccatgtgg agcgcactgc tgcaggagca cggtggcccc
     2761 atgctgttcg gtgccttcac ggtggccgat gcctactttg ccccggtgtg catgcgcatc
     2821 cagacctacg ccctgccagt gccgccggtg gtgcaggcct atatcgaccg cgtctgtgcc
     2881 ctgcccggcg tgaaggcatg gatcgatgat gcacgggccg agcaggattt tctggacttt
     2941 gaagagccct accggctcca gcgctgaact gggccacgcg gcatgcagat ctacctggtt
     3001 ggcggtgcgg tgcgcgatgc cctgatgggc ctgcgcaacc a
//
LOCUS       DAOSJM010000306         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28287_length_3025_cov_4.006397, whole genome shotgun sequence.
ACCESSION   DAOSJM010000306 DAOSJM010000000
VERSION     DAOSJM010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28287_length_3025_cov_4.006397"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2203)
                     /locus_tag="PKX82_10335"
     CDS             complement(<1..2203)
                     /locus_tag="PKX82_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOF52696.1"
                     /translation="MNVSAWSIRNPIAALMLFVMLTFAGLLSFNAMKVQQFPDLDLPT
                     ITVVASLPGAAPAQLETEVARKLENSIATLQGLKHIYTKVQDGGVTITAEFRLEKPGQ
                     EAVDDVRSAVAQVRADLPADLRDPVVNKLNLAGSPVLAFTVGSSQLDDEALSWFVDNT
                     IAKRLLSVRGVGAINRVGGADRQVQVALDPMRLQALGATAADISRQLKLVQQDTAGGR
                     TDLGGSEQPVRTLGKVASARELEALELSLPDGRRIRLDQVATIRDTIAEPRSAALLNG
                     KPVVGFEVARSKGASEVEVGAAVQKALAELRALHPEIEITEAFDFVSPVQEEYEGSMA
                     LLYEGAVLAVLVVWLFLRDWRATFVSAVALPLSAIPAFIGMHYLGFSVNVITLLALSL
                     VIGILVDDAIVEVENIVRHLRMGKTPYQAAMEAADEIGLAVIATTFTLIAVFLPTAFM
                     SGIPGKFFKQFGWTAALAVAASLLVARMLTPMMAAYILKPIVTPHQDPRWMTLYMRWA
                     TWCVRHRWITMLAAGAFFVGSLMLIPLLPKGFIPPDDNSQTQVYLELPPGATLAQTRA
                     TAEAARVAITAVPHVKSVYTTIGGGLAGSDPFTPKGAAETRKATLTILLDARGTRPLK
                     QGIEHQIRTALEPLPGARSKVGLGASGEKYILVLTGEDANALNAAARAVEKDLRTIEG
                     LGNIASTASLVRPEIAVRPDVARAADLGVTSTAIGETLRIATQGDYDAALPK"
     gene            complement(2216..>3025)
                     /locus_tag="PKX82_10340"
     CDS             complement(2216..>3025)
                     /locus_tag="PKX82_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOF52697.1"
                     /translation="KAQAQAAEATANADRARALQPTGVLSGQQTNQLLTAELTAKARL
                     ESAKATLAVQQLRLKHTQVLAPDNGIISARNATVGAVVGAGTELFRLIRQGRLEWRAE
                     VTATELGRVSPGGKVSLTTASGATVQGKVRMVGPTVDPQTRNALVYVDLESSANGAAR
                     AGMFARGEFELGSTGALTVPQQAVVVRDGFTYVFKVGADNRVAQQKVDTGRRIGDQVE
                     ILQGLTAEATIAVAGAGFLNDGDTVRIGTAPVASVPNPAPARVKSAQPATK"
BASE COUNT          475 a         1003 c         1013 g          534 t
ORIGIN      
        1 gcttgggcag ggcagcgtcg tagtcaccct gggtggcaat gcgcagggtc tcgccaatgg
       61 cggtgctggt cacaccgagg tccgctgcgc gggccacgtc ggggcgcacc gcaatctctg
      121 ggcggaccag gctggcggtg gatgcgatgt tgcccagacc ctcaatggtg cgcaggtctt
      181 tctccacagc gcgcgccgcg gcattcagtg cgttggcatc ctcgccggtc agtaccagga
      241 tgtatttctc acccgaagcc cccagcccca ccttgctgcg cgcgccgggc agcggctcca
      301 gtgcggtgcg gatctggtgc tcaatgccct gcttgagcgg gcgggtgccc cgcgcgtcga
      361 gcaggatggt cagtgtggcc ttgcgcgttt cggccgcacc tttgggggtg aacggatcac
      421 tgccggccaa cccgccgcca atggtggtgt aaacgctctt gacgtgcggt accgcggtga
      481 tggccacgcg ggcggcttcg gcagtagcgc gggtctgggc cagtgtggcc cctggcggca
      541 gctccaggta gacctgggtt tgcgagttgt cgtctggtgg aatgaagccc ttgggcagca
      601 gcggtatcag catcagtgag cccacgaaga aggcgccagc cgccagcatc gtgatccacc
      661 ggtgccgcac gcaccaggtg gcccagcgca tgtacagcgt catccagcgt gggtcctggt
      721 gcggtgtgac gatgggcttg aggatgtagg ccgccatcat gggcgtgagc atccgcgcca
      781 ccagcagcga ggccgccacc gccagcgccg ccgtccagcc aaactgcttg aagaacttgc
      841 ccggaatgcc gctcatgaac gcggtcggca ggaacacggc aatcagcgtg aaggtggtgg
      901 caatcacggc caggccgatt tcgtcagcgg cttccatggc ggcctggtag ggtgtcttgc
      961 ccatgcgcag gtggcgcacg atgttctcca cctccacaat cgcatcgtcc accagaatgc
     1021 cgatgaccag cgagagcgcc agcagcgtga tcacgttgac gctgaacccg aggtagtgca
     1081 tgccgatgaa ggccgggatg gccgacagtg gcagcgccac ggcagacaca aaagtggcac
     1141 gccagtcgcg cagaaacagc cacaccacca gcaccgccag cacggcacct tcgtacagca
     1201 gggccatgga accttcgtac tcctcctgca ccggcgacac aaagtcaaac gcttcggtga
     1261 tttcaatctc ggggtgcagg gcacgcagtt cggccagggc cttttgcacc gccgcaccga
     1321 cttccacttc actcgcgccc ttgctgcggg ccacttcaaa gcccaccacc ggcttgccgt
     1381 tgagcaatgc cgccgagcgg ggctcggcaa tggtgtcgcg gatggtggcc acctggtcca
     1441 gacgaatgcg ccgaccgtcg ggcagggaga gttccagcgc ttcgagttcc cgggccgagg
     1501 ccactttgcc cagcgtgcgc acgggttgtt cgctgccgcc cagatcggtg cgtccaccgg
     1561 cagtgtcctg ctgtaccagc ttgagctggc gcgagatgtc ggccgccgtg gcgcccaggg
     1621 cctgcagccg catcgggtcc agcgccacct gcacctggcg gtcggcgccg cccacgcggt
     1681 tgatagcacc cacgccgcgc accgacagca gccgcttggc aatggtgttg tccacgaacc
     1741 agctcagcgc ttcatcatcg agctggctgg agcccacggt aaacgccagt accgggctgc
     1801 ccgccagatt gagtttgttg accaccgggt cgcgcaggtc ggccggcagg tccgcacgca
     1861 cctgggccac ggccgagcgc acgtcatcca ccgcttcctg tccgggcttt tccagccgga
     1921 actcggcggt gatggtgacg ccgccatcct gcaccttggt gtagatgtgc ttgaggccct
     1981 gcagcgtggc aatggagttc tccagcttgc gggccacctc ggtttccagt tgggccggtg
     2041 cggcgccggg gagggacgcc actaccgtga tggtgggcag gtccaggtcc gggaactgct
     2101 gcaccttcat ggcattgaac gacagcagcc ccgcgaacgt gagcatcacg aacagcatca
     2161 gggcggcaat ggggttccgg atcgaccagg ccgatacatt catggcaact gctccttatt
     2221 tagtagcagg ctgcgctgat ttcacgcggg ctggggccgg atttggcact gaagccaccg
     2281 gcgcggtgcc tatgcgcacc gtgtcgccgt cgttgaggaa gcccgcaccc gccactgcga
     2341 tggtggcttc tgcggtgagg ccctgcagaa tttccacctg gtcgccaatg cgccgaccgg
     2401 tgtccacctt ttgctgcgcc acgcggttgt ccgcgcccac cttgaagaca taggtaaacc
     2461 cgtctcgcac caccaccgcc tgctgcggta ccgtcagcgc gccggtgctg cccagttcaa
     2521 attcgccccg cgcgaacatg cccgcgcgtg cggcaccgtt ggcgctagac tccaggtcca
     2581 catagaccag cgcgttgcgc gtctgcgggt ccaccgtggg gccgaccatg cgcaccttgc
     2641 cctgcacggt ggcgccgctg gcggtggtca ggcttacctt gccaccgggg ctgacgcggc
     2701 ccagttcggt ggcggtcact tcggcacgcc attccaggcg cccctggcgg atcagccgga
     2761 acagttcggt ccctgcgccc accactgcgc ccacggtggc gttgcgggca gagatgatgc
     2821 cgttgtccgg tgcaagcacc tgggtgtgtt tgaggcgcag ctgctgcacc gccagggtgg
     2881 ctttggcact ctccaggcgt gccttggcgg tgagttctgc ggtcagcagc tggttggtct
     2941 gctggccact gaggacgccg gtgggctgca gggcgcgggc ccggtcggcg ttggcggtgg
     3001 cttccgcagc ctgcgcctgg gcttt
//
LOCUS       DAOSJM010000307         3025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28289_length_3025_cov_3.884512, whole genome shotgun sequence.
ACCESSION   DAOSJM010000307 DAOSJM010000000
VERSION     DAOSJM010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28289_length_3025_cov_3.884512"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2398)
                     /gene="gcvP"
                     /locus_tag="PKX82_10345"
     CDS             complement(<1..2398)
                     /gene="gcvP"
                     /locus_tag="PKX82_10345"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOF52698.1"
                     /translation="MPSTPSLAQLEDASEFVARHIGISASDEALMLQAIGEKSRKELI
                     DSIVPRSIARSSKMAIPAAITEAAALAELKALAGKNQQFKSFIGQGYYGTHTPGVILR
                     NILENPAWYTAYTPYQAEISQGRMEALVNFQTMVCDLTGMPMANASMLDEATAAAEAM
                     TLAKRSVKSKSNVFVVAGDCHPQTIEVVQTRAQPLGIEVRIANSAAEWETLVAGDYFA
                     VLAQYPATSGRIDDLPGEVAKVHAKQAAFIVAADLLALTLLTPPGEWNADIVIGTTQR
                     FGMPLCNGGPHAAYMACRDEYKRSMPGRLVGVSVDTHGNPAYRLALQTREQHIRREKA
                     TSNICTAQVLPAVVASMYAVYHGPAGLTRIAQRVATYTAILVKGLQALGHTPTCASAF
                     DTVTLHTGDATNSIAARAISAGANLRIYFKEYIAVSLDETTTRDDIALLWSFFAKPGQ
                     ALPRVEDFAADAAPLIPAALRRTSKFLTHPVFNTYHSETGMLRYIRMLSDKDLALDRS
                     MIPLGSCTMKLNATSEMIPITWPEFANIHPFAPADQRQGYTELDEQLRAWLCAATGYA
                     GISLQPNAGSQGEYAGLLVIKAFHESKGQGHRNICLIPSSAHGTNPASAMMVGMTVVV
                     TQCDSNGNVDMADLQAKCEQHSANLAAVMITYPSTHGVFETSVKELCALVHQHGGRVY
                     VDGANMNALVGTAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVCVVEDLVPFLPGH
                     ATGGVAGTGAVSAAPLGNAAVLPISWMYCRMMGPDGLRHATEAAILAANYISARLADH
                     Y"
     gene            complement(2516..2887)
                     /gene="gcvH"
                     /locus_tag="PKX82_10350"
     CDS             complement(2516..2887)
                     /gene="gcvH"
                     /locus_tag="PKX82_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOF52699.1"
                     /translation="MTIKYTPDHEWLSVDGDTATVGITHHAQDALGDVVFVDLPEVGA
                     SIDAKAVAGVVESVKAAADVYMPVTGTITEVNEALRADPSLANSDPLGAGWFFKVKLS
                     NPSELDALMDETSYTAFSAAA"
BASE COUNT          511 a          860 c         1074 g          580 t
ORIGIN      
        1 ggtagtggtc cgccaggcgc gcgctgatgt agttggccgc caggatggcc gcctcggtcg
       61 catggcgcag gccgtccggg cccatcatgc ggcagtacat ccagctgatc ggcagcacgg
      121 cggcattgcc cagtggcgct gcgctcacgg cgccggtgcc agccacaccg ccagtggcat
      181 gtccgggcag gaaaggcacc aggtcttcca ccacgcagac ggggccgaca ccggggccgc
      241 caccgccgtg ggggatgcag aaggtcttgt gcaggttcag gtggctgacg tcgccgccaa
      301 actcgccggg ggcggcggtg ccgaccagcg cattcatgtt ggcgccatcc acatagacgc
      361 ggccgccgtg ctggtgcacc agcgcgcaca gctctttcac cgatgtttca aacacgccgt
      421 gggtgctggg gtaggtgatc atcaccgcgg ccaggttggc gctgtgctgc tcgcacttgg
      481 cctgcaggtc ggccatgtcc acgttgccgt tggagtcgca ttgggtgacc acgacggtca
      541 tgcccaccat catggcgctg gccgggttgg tgccgtgcgc gctgctgggg atcaggcaga
      601 tattgcggtg gccctggccc ttgctttcgt ggaaagcctt gatgaccagc aggcccgcgt
      661 attcgccctg gctgcccgcg ttgggctgca ggctgatgcc ggcgtagccg gtggcggcgc
      721 agagccaggc gcgcagctgt tcgtccagct cggtataacc ctggcgctgg tcggcggggg
      781 caaaggggtg gatgttggca aactcgggcc aggtgatggg aatcatctcg ctggtggcgt
      841 tgagcttcat ggtgcaggag cccagcggaa tcatgctgcg gtccagcgcc aggtccttgt
      901 cggacagcat gcggatgtag cgcagcatgc cggtctcgga gtggtaggtg ttgaagaccg
      961 ggtgcgtcag gaacttggac gtgcggcgca gcgcggcggg gatcagcgga gcggcatcag
     1021 cagcaaagtc ctccaccctg ggcagggcct ggccgggctt ggcaaaaaac gaccaaagca
     1081 gcgcaatgtc gtcgcgggta gtggtctcgt ccagagacac agcgatgtac tctttgaagt
     1141 aaatccgcag gttagccccc gccgatattg cgcgggcagc tatcgaattg gtagcatccc
     1201 cggtgtggag ggtgacagtg tcaaatgccg aggcacaggt gggcgtgtgg cccagggcct
     1261 gcaatccctt gaccagaatc gcggtatagg tggctacgcg ttgtgcgatg cgtgtgagtc
     1321 ctgcagggcc gtggtacacg gcgtacatgc tggccaccac cgccggcagc acctgcgcag
     1381 tacagatgtt ggacgtggct ttttcgcggc ggatgtgttg ttcgcgggtt tgcagggcca
     1441 ggcggtaggc ggggttgccg tgggtgtcca cactgacacc caccaggcga ccgggcatgg
     1501 agcgtttgta ttcatcgcgg caggccatgt aggcagcgtg cgggccgccg ttgcacagcg
     1561 gcatgccaaa gcgctgggtg gtgccgatca caatgtcggc gttccattct ccggggggcg
     1621 tcagcagggt tagtgccagc aggtcggccg ctacgatgaa ggcggcctgt ttggcgtgca
     1681 ccttggcaac ttcgccgggc aggtcgtcaa tgcgtccgct cgttgcgggg tattgggcca
     1741 gcaccgcaaa gtagtcgcca gcgaccaagg tttcccactc ggctgcggag ttggcgattc
     1801 ggacctcaat acccagaggc tgggcgcggg tctgcaccac ttcgatggtt tgggggtggc
     1861 agtcgccggc caccacaaac acattgctct tgcttttgac gctgcgcttg gccagcgtca
     1921 tggcttcggc cgccgcagtg gcttcgtcca gcatggacgc gttggccatg ggcatgccgg
     1981 tcaggtcgca gaccatggtc tggaagttga ccagcgcctc catacggccc tgggaaatct
     2041 cggcctggta gggcgtgtag gcggtgtacc aggcggggtt ttccaggatg ttgcgcagga
     2101 ttacaccggg cgtatgggtg ccgtaatacc cttgaccgat gaagctcttg aactgctggt
     2161 tcttgcctgc cagcgccttg agttccgcca gtgccgccgc ttcggtaatc gctgcgggaa
     2221 ttgccatctt gctagagcgc gcgatggaac gcggcacgat gctgtcgatc agttccttgc
     2281 gggacttttc gccaatggct tgcagcatca gcgcttcatc ggaagcagag atgccaatgt
     2341 ggcgtgcaac aaattcgctg gcgtcttcga gttgggcgag ggaaggtgtc gagggcatgg
     2401 tcttggagca atgttgttgt cggaggtgag ggcaattttc cgctgggccg ccccaaggaa
     2461 aattagcccc cttggggggc agcgacccgc gcagcggcgg agcgtggggg ccagttcagg
     2521 cagcggcgga gaaggcggtg tagctggtct cgtccatcag cgcgtcgagt tcagacgggt
     2581 tggacagctt gaccttgaag aaccagccgg cgcccagcgg gtcgctgttg gccagcgacg
     2641 ggtcggcacg cagggcttcg ttgacttcgg tgatggtgcc ggtgacaggc atgtagacat
     2701 cggcagcggc cttgacggac tccaccacgc cggccacggc cttggcgtca atggatgcgc
     2761 ccacttcggg caggtccaca aacaccacgt cgcccagcgc gtcctgcgcg tggtgggtga
     2821 tgccgacggt ggcggtgtcg ccgtcaacgc tgagccattc gtggtcgggg gtgtacttga
     2881 tggtcatgaa aaactcctga aacgtgaaat tgaaataggt ggaaaaacca aaaattgaac
     2941 ctgccagcgc agactgtagc cgctggcgat ggcggcatca gccgcggtag tagcgggtgg
     3001 gcacaaaagg catggcgctg acttc
//
LOCUS       DAOSJM010000308         3021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28343_length_3021_cov_4.272421, whole genome shotgun sequence.
ACCESSION   DAOSJM010000308 DAOSJM010000000
VERSION     DAOSJM010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28343_length_3021_cov_4.272421"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PKX82_10355"
     CDS             complement(<1..993)
                     /locus_tag="PKX82_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF52700.1"
                     /translation="MTVAQQVLIVDDTEINLILFAALVKKLGDCESHSFSDSRAGLAW
                     VQQYVPDLVIVDYMMPEMDGLEFIRLLREIPGREAIPVLMITANDQKQIRYRALDIGA
                     NDFLTKPVDKVEFLARAKNMLTLNDARKKLADQAAWLAEEVRKATHEVVERERETVIR
                     LSRAAEFRDPETGAHILRMAHFSQIIARGMGLSESDQYLLLEAAPMHDIGKVGIADKI
                     LLKPGRLEPEEFEIMKRHATIGYELLQGSSSRVLQAGAEIARGHHEKFDGSGYPQGLK
                     GEEIPIFSRIVAVADVFDALTSERPYKKAWDVEAAVDFLKAGSGSHFDPACVTAF"
     gene            complement(1035..>3021)
                     /locus_tag="PKX82_10360"
     CDS             complement(1035..>3021)
                     /locus_tag="PKX82_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF52701.1"
                     /translation="GNIIFNGYDRAELHKAKEDAESASHAKSDFLANMSHEIRTPMNG
                     VIGMTDLLMDTELDAEQSEYVSIVKSSADALLRVINDILDFSKIEAGKLQIEHIPFHL
                     GRTVDETLKTLAMRANDKGLELVCDMDPDVPMALVGDPGRLRQVLVNLIGNALKFTAS
                     GEVVLRVSEERSSGSECQLHLAVSDTGIGIATDKLGSIFEAFSQEDSSITRKYGGTGL
                     GLTICSRLVEAMGGHIWVESELGKGSVFHFTVRLQRDAAGASAEPQPVDLAGTTMLLV
                     DDNAVNLQVLARGLEAAGVDVHAVDSGAGALQWLSARELAGAPCDLVVLDAQMPGMDG
                     FEVAQQILKIPRWGRVPMVMLSSAGLKGDAQRSREAGISGYASKPIARHELLQVLARV
                     LNLEAREAQVLVTRHSIRDADAGMEVLLVEDHAINQKLAVVLLERWGHRVTVADNGQL
                     ALDAMARQRFDVVLMDMMMPVMDGLEATRRIRASETGQHVPIIAMTANAMESDRQRCI
                     DAGMDDYLSKPIKALELQELLRKYSAEGYSSVPASLEEAPSAAPAAFNYAAGLAAMDQ
                     EIREIIAQAFVDQWPQDQQKLRTGVGKGDMLAVLHTAHSLKGTLSMFGARPASELARQ
                     VESLAGLSDAQGVSVLIDTLCAEVELLLAAIAADIPA"
BASE COUNT          611 a          972 c          811 g          627 t
ORIGIN      
        1 aaatgccgtg acgcacgcag gatcgaagtg gctgccggag cccgccttca aaaaatcgac
       61 ggcggcctcg acgtcccagg cttttttgta ggggcgctcg gatgtcagcg catcaaacac
      121 atctgccacc gccacaatac ggctgaaaat cggaatttcc tctcccttga gtccttgcgg
      181 gtacccggaa ccatcaaact tttcatggtg gccacgggcg atctcggcgc ctgcctgcag
      241 aacccgcgac gagctgccct ggagcaactc gtaaccgatc gtggcatggc gtttcatgat
      301 ttcaaactcc tcgggctcca ggcgtccggg tttgagtaat atcttgtctg cgatgcccac
      361 tttgccgatg tcgtgcatgg gcgcagcttc cagcaacaga tactgatcgc tctccgacaa
      421 ccccattccg cgcgcaatga tttgcgaaaa atgggccatg cgcagaatat gggcaccggt
      481 ttctggatca cggaattcgg ccgctctgga caggcgaatc acggtttcgc gctcacgctc
      541 caccacttca tgggtcgcct tgcgtacttc ttcggccagc caggccgcct ggtccgcaag
      601 ctttttccgc gcatcgttga gtgtcagcat gttcttggca cgtgccagga actcgacctt
      661 gtctaccggc ttggtcaaaa agtcgttggc cccgatatcc agtgcgcggt aacggatctg
      721 cttctggtcg ttggccgtga tcatcaacac cggaatagcc tcgcgaccgg ggatttcccg
      781 gagcaagcgg atgaactcca ggccatccat ctccggcatc atgtaatcca cgatgacgag
      841 gtccggcaca tattgctgca cccacgccag tccagcacgg gaatcggaaa aactgtggga
      901 ttcgcaatcc ccaagttttt tgacaagggc tgcaaaaaga atcagattga tttcagtgtc
      961 atcgacgatc aaaacctgtt gagccacagt catttaccct ttacatcgaa tgtgcatctt
     1021 agcgtctgtt gcgctcatgc ggggatgtcc gcagcaatgg ccgccagcag cagttccact
     1081 tccgcgcaaa gcgtgtcgat cagaacgctg acgccctgcg catccgacag gccggcaagt
     1141 gactccacct gacgggccag ttcactggcg ggacgcgcac caaacatgga aagcgtcccc
     1201 ttgagcgaat gagccgtatg caacacggcc agcatgtcac cctttccgac cccagtgcgc
     1261 aatttctgct ggtcctgcgg ccactggtct acaaacgcct gggcaatgat ttcccgaatt
     1321 tcctggtcca tggcagccag tcctgcggcg taattgaatg cggccggtgc tgcagaagga
     1381 gcctcctcca gcgaagccgg cacactgctg tagccttctg cggagtattt cctgagtagt
     1441 tcctgcaatt ccagagcctt gatgggcttg gacaggtagt catccatgcc cgcatcgatg
     1501 cagcgctggc ggtccgactc catggcattg gccgtcatgg caatgatggg aacatgctgg
     1561 cctgtttcag atgcccggat acgccgcgtg gcctccagtc catccatcac cggcatcatc
     1621 atgtccatca gaacgacgtc aaaccgctgc cgtgccatcg cgtccagggc cagttgaccg
     1681 ttgtctgcca ctgtcacccg gtggccccac cgctctagca ataccaccgc cagcttctgg
     1741 ttgatcgcat ggtcttccac cagcaggacc tccatgccgg catcggcgtc gcgtatggag
     1801 tgacgtgtca ccaggacttg cgcttcccgc gcctccagat tcagcacacg ggccagcact
     1861 tgcagcagtt cgtggcgtgc aatgggtttg gacgcgtagc cggatatccc ggcctcgcgc
     1921 gagcgttgcg catcgccttt gagaccagcc gacgacagca tcaccatcgg cacccggccc
     1981 caacggggta ttttcagaat ctgctgcgcc acctcaaagc cgtccatgcc aggcatctgc
     2041 gcatcgagca caaccaaatc gcaaggtgca cccgccaact ccctggccga cagccactgc
     2101 agggcgccag cccctgaatc taccgcgtga acgtctacgc cagcggcttc caggccacgc
     2161 gcaagcacct ggaggttgac ggcgttgtcg tccaccagca gcatcgtggt gccagcaaga
     2221 tcaacgggct gtggctccgc actggcgcca gcggcatctc gctgcaaccg caccgtgaag
     2281 tgaaacacgc tgcccttgcc caactcgctc tcgacccaga tgtgcccgcc catggcttcg
     2341 accagccggg agcagatggt caagcccagt ccggtaccac cgtacttgcg ggtgatggag
     2401 ctgtcttcct gcgagaacgc ctcgaaaatc gagcccaact tgtccgtcgc aatgccaatg
     2461 ccggtatcgc tgaccgccag gtggagctgg cattcgctgc cagaactccg ctcctcggaa
     2521 acacgcaaaa ccacttctcc gctggcggtg aacttcaatg cattgccaat caggttgacc
     2581 aggacctgcc gcaagcggcc cggatccccc accaacgcca tgggcacgtc cggatccatg
     2641 tcgcagacca gttccagccc tttgtcgttg gcacgcatgg ccagcgtctt gagggtttcg
     2701 tccaccgtcc gccccagatg gaacgggatg tgctctatct gcagcttgcc agcctcgatt
     2761 ttggagaaat ccagaatgtc attgatcacc cgcaacaggg catccgcaga cgacttcacg
     2821 atcgaaacgt actcgctctg ctcggcgtcc agctcggtat ccatcagcag gtccgtcatg
     2881 ccgatgacgc cattcatcgg ggtgcggatt tcatggctca tattggccag aaaatcggac
     2941 ttggcatggc tggctgactc ggcatcttcc ttggctttgt gcagttcggc cctgtcgtaa
     3001 ccgttgaata taatattacc a
//
LOCUS       DAOSJM010000309         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28369_length_3019_cov_3.571525, whole genome shotgun sequence.
ACCESSION   DAOSJM010000309 DAOSJM010000000
VERSION     DAOSJM010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28369_length_3019_cov_3.571525"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1317)
                     /locus_tag="PKX82_10365"
     CDS             complement(61..1317)
                     /locus_tag="PKX82_10365"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOF52702.1"
                     /translation="MKRVDDFRLKLGNKELVPLIIGGMGVDISTAELALEAARLGGIG
                     HISDAMVQDVSDRRFDTEFVKEKTKFYKHNIDNSDKSIVQFDLGRLAEATKLHVGHTM
                     EAKRGDGMVFINCMEKLTMNGPKETLQVRLNSALDAGIDGITLSAGLHLGSFALMADH
                     PRFRDAKLGIIVSSVRALQLFLRKTARLSRLPDFVVVEGPLAGGHLGFGMDWAQYDLA
                     TIVEEILQFLKTEQLEIPLIAAGGIFTGSDAVSFLELGAAGLQVATRFTVANECGLPS
                     KVKQEYFRASEEDIIVNTISPTGYPMRMLRNTPAIGAGIRPNCEAYGYLLDGSGGCAY
                     ITAYNEQIALHPDGKNISVMDKTCLCTHMRNYNCWTCGSTTYRLKDTTRRLADGSYQE
                     LSAEHIFQDYQYSTNQQIALPDAIGT"
     gene            1436..2074
                     /locus_tag="PKX82_10370"
     CDS             1436..2074
                     /locus_tag="PKX82_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52703.1"
                     /translation="MLCYQTIPVTAFQQNCSLVWCDQTRRAAVIDPGGDLDVILNQVA
                     ERELSLEAIWLTHAHIDHAGGTGELSRRLNIPIVGPHKADQFWIDALPKQSAMFGFPA
                     AQSFQPTQWLEDGDTVTVGQSSLQVRHCPGHTPGHVVFHSAEAKRAFVGDVLFAGSIG
                     RTDFPQGDLDTLLDSIRSRLWPMGDETVFIPGHGPESTFGRERRTNPYVSDI"
     gene            2118..>3019
                     /locus_tag="PKX82_10375"
     CDS             2118..>3019
                     /locus_tag="PKX82_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HOF52704.1"
                     /translation="MAKKYSLSTKLVAIGAILLIVALTSIGLTLWVTWQLEGGAAAVN
                     EAGRMRMQSWRLASTAQANPSTEAVQELVAKFDASLALLKEGDPSRPLFVPWDDNVRA
                     RFESVSRLWAEQRSRWTGTRPPTAQESLQATTEFVDAIDGLVSAIEQQMSRLTAILNL
                     FQFVMMALAIAGAVIMLYTGYLYVINPMANLQQGLRKIESGDFSTRIEMEALDEFGLV
                     ALGFNGMAGKLQTMYDGLEAQVEAKTRRIEAQRARIETLYEVSAFLASANTIQELSQG
                     FAQRVRKVLNADAVAVRWSDEANQR"
BASE COUNT          630 a          838 c          868 g          683 t
ORIGIN      
        1 tggtaccaga tccatccgga cagacaggca acctgaacac tctccgcgac aggtccgttt
       61 tcaggtcccg atggcgtcag gcaaagcaat ctgctggttg gtgctgtact gatagtcttg
      121 gaagatatgc tccgcactga gctcctggta actgccatcg gcaaggcggc gcgttgtatc
      181 tttcagtcgg taggtggtgc tgccacaggt ccagcagttg tagttgcgca tgtgggtgca
      241 caggcaggtc ttgtccatca ccgagatgtt tttcccatcc ggatgcaggg caatctgttc
      301 gttgtacgcc gtgatgtagg cgcatccgcc tgacccgtcc agaaggtagc cgtacgcttc
      361 gcagttgggg cgaatgcctg cgccaatggc gggtgtgttg cgcagcatgc gcatcgggta
      421 gccagtgggg gagatggtgt tgacaatgat gtcctcttca ctcgcccgga aatactcctg
      481 cttcaccttg gaggggaggc cgcattcatt ggcaactgtg aacctggtgg ccacctgcaa
      541 tccggctgca cccagctcca gaaaagacac ggcatcgctg ccggtgaaga tgcctcccgc
      601 agcaatcagc ggaatttcca gttgttcggt cttcaggaat tggagtattt cttccacgat
      661 agtcgccaga tcgtattgcg cccaatccat gccaaagccc aagtgcccac cggccaatgg
      721 accttcaacc accacaaagt cgggcaggcg ggaaaggcga gcggtcttgc gaagaaacaa
      781 ttgcaaagcg cgcaccgacg acacaatgat gcccagcttg gcatcgcgaa agcgggggtg
      841 atctgccatc agggcaaatg aacccagatg gagccccgcg ctgagcgtga tgccgtcaat
      901 gcccgcgtcc agcgccgaat tgagccggac ctgcaatgtc tccttcggcc cgttcatggt
      961 gagcttttcc atgcagttga tgaaaaccat gccgtcgcca cgtttggcct ccatggtgtg
     1021 gcctacatgc agcttggtgg cttccgcaag gcggcccaaa tcaaactgca caatgctctt
     1081 gtccgagttg tcgatgttgt gcttgtagaa cttggtcttt tccttcacga attccgtgtc
     1141 aaaccgccga tccgatacgt cctgcaccat ggcgtctgaa atatggccta tgccacctag
     1201 ccgtgcggct tcaagggcca gctccgcggt ggagatatcc acgcccattc caccaataat
     1261 cagtggcacc aattctttgt tgcctaactt gaggcggaaa tcatctactc gtttcatcgc
     1321 tatccccttt gggcgcaagt attaaccaaa ccgccacgtt ttttcaccgc aatggcaatt
     1381 acccatgcgc ggagtacaaa atggcaagaa tcattgaaat tgtcatcaat ctgccatgct
     1441 ttgctaccag accattcccg taaccgcctt ccagcagaac tgctctctgg tgtggtgtga
     1501 ccagacgcgg cgggctgccg tcattgatcc gggtggagat ctggacgtga ttttgaacca
     1561 ggtggctgaa cgggagcttt cgctggaggc catttggttg acgcacgcac atattgacca
     1621 tgcgggcggg acaggtgagc tttcgcgtcg actcaatatt cccattgtcg ggccccataa
     1681 ggcagaccag ttctggattg atgcgttacc caagcaaagt gccatgttcg gttttccggc
     1741 agcgcagtcc ttccagccaa cgcaatggct ggaagatggt gacaccgtga ctgtcgggca
     1801 gagttctctg caggtacgcc actgccccgg tcatacgccg ggccatgtgg tattccattc
     1861 ggcggaggca aagcgtgctt ttgtgggtga tgtcctgttt gcaggatcca ttgggcgcac
     1921 cgatttcccc cagggcgatc tggatacgtt gttggacagt atccgctccc ggctttggcc
     1981 tatgggtgac gaaaccgtgt tcattccggg acatggtccc gagagtactt ttggacggga
     2041 acgccggaca aacccctacg tgtcagatat ttgactgacg gctcccccac tttggaccgg
     2101 caggttaccc ttaacgcatg gcaaaaaaat attccttatc caccaaactg gttgccatcg
     2161 gtgccatcct tctgatcgtg gcattgactt cgattgggtt gaccctgtgg gtgacctggc
     2221 aactggaagg cggagctgct gcggtcaacg aggcaggccg catgcgcatg caatcctggc
     2281 ggctggccag taccgcccaa gccaaccctt ccacggaggc ggtgcaagaa ctggttgcta
     2341 aatttgatgc cagcctggca ttgctgaaag agggcgatcc cagcagaccg ctgtttgttc
     2401 cctgggatga caatgtccgt gcgcggtttg aatccgtcag tcgtttgtgg gctgagcagc
     2461 gcagtcgctg gaccggaacg cgtccaccaa cagcgcagga gtcacttcaa gcaaccaccg
     2521 agtttgtgga cgccattgac ggcctggtat ctgcgattga acagcagatg tcgcgactga
     2581 cagcgatcct gaacctgttt cagttcgtca tgatggccct ggcgattgcg ggcgcagtca
     2641 tcatgctgta caccggctat ctctacgtca tcaacccaat ggccaacttg cagcaggggt
     2701 tgcgcaagat tgaatcgggc gatttttcga cgcgcatcga aatggaggct ctggacgagt
     2761 tcgggttggt ggcgcttggc ttcaatggaa tggccggtaa attgcagaca atgtacgacg
     2821 gcctggaggc gcaggtcgaa gccaaaacgc ggcggattga agcccagcgt gcgcggatcg
     2881 aaaccctcta tgaggtcagt gcatttctcg ccagtgccaa taccatccag gaactgtctc
     2941 aggggtttgc gcagcgggtg cgcaaggtgc tcaatgcaga tgctgttgcc gtgcgatggt
     3001 cggacgaggc caatcaacg
//
LOCUS       DAOSJM010000310         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28480_length_3010_cov_3.119459, whole genome shotgun sequence.
ACCESSION   DAOSJM010000310 DAOSJM010000000
VERSION     DAOSJM010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28480_length_3010_cov_3.119459"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..337)
                     /locus_tag="PKX82_10380"
     CDS             complement(35..337)
                     /locus_tag="PKX82_10380"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOF52705.1"
                     /translation="MILELADIRIHPGQNAAFDEAITRGLRDVISQAKGFRGYKVNRG
                     IESPERYILQIFWDTLEDHTVGFRQSDAFTQWRAIVGPFFAVPPVVEHFDLLTKSV"
     gene            447..1112
                     /gene="maiA"
                     /locus_tag="PKX82_10385"
     CDS             447..1112
                     /gene="maiA"
                     /locus_tag="PKX82_10385"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HOF52706.1"
                     /translation="MKLHNYFRSSASFRVRIALALKGLPYQYIAVNLAQGDHLLDAYR
                     SQSPEVLVPLLEIDGHRLSQSMAIIEYLDETRPEPPLLPDDPLGRAHVRALAQSVACE
                     IHPLNNLRVLKYLSRELKVDEDTKNTWYRHWVRGGLEAFERQLLAQDALRSAAGQPPS
                     VYCWGASPTMADCLLVPQIFNAQRFHVNLDGLPRTMTAFEACMQLQAFQDAQPSSCPD
                     FVA"
     gene            1115..1915
                     /gene="pgeF"
                     /locus_tag="PKX82_10390"
     CDS             1115..1915
                     /gene="pgeF"
                     /locus_tag="PKX82_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOF52707.1"
                     /translation="MGGLHLPAGVDWLVPQWPAPARVKAVCTTRQGGKSEAPWNSLNL
                     GTHVGDTPDSVQHNRAVLQTAMGTQLVFLNQVHGSHMAALSPDTADGYEADGAWTDQT
                     GLACVVMVADCLPVLLCDTQGRRVAAAHAGWRGLVGTDGKGILEASLEPFRPLAPVEY
                     AQGATEIIAWLGPCIGPEAFEVGDEVRTVFVDVSPEADQCFRPLGQGKWLADLAGLAR
                     QRLQALGVTGIYGNDSTAAWCTVTNPSRFFSHRRDRVSGRQAACIWLE"
     gene            complement(1847..2119)
                     /locus_tag="PKX82_10395"
     CDS             complement(1847..2119)
                     /locus_tag="PKX82_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52708.1"
                     /translation="MYIIPIAWIYVAFMMAAAEATNTNGSVLGAIITFVLYGMLPVGL
                     MLYFMGTPARKRRIREREAAERLASLQPDAGGLPPTDAVPPVGKEP"
     gene            2213..>3010
                     /locus_tag="PKX82_10400"
     CDS             2213..>3010
                     /locus_tag="PKX82_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOF52709.1"
                     /translation="MTTQTPNDFWTQASQQFQQSMSESWGKAIQSFQTMDIGSAGASM
                     PSMGKKLPEIHFAPEKLQELQQQYMQEALALFNSGMTPPDTKSDKRFADAAWGDNPVA
                     AYAAAVYLLNARTMMGLAEAVDADPKTRNRIRFAIEQWMAAAAPSNFMAFNAEAQKKA
                     IETKGESIAKGMKNLMHDLQQGHVSMTDESLFEVGKNVATTEGAVVFENELFQLIEYK
                     PLTAKVYEKPFLMVPPCINKFYILDLQPDNSLVRYAVAQGHRTFVVSW"
BASE COUNT          592 a          895 c          945 g          578 t
ORIGIN      
        1 acaaaagaca tgcgtgtact ccagtcaggt gggatcagac cgacttggtc aggaggtcga
       61 agtgctccac aacgggcgga accgcaaaga acgggcccac gatggcacgc cactgggtga
      121 acgcgtccga ctgccggaag cccacagtgt ggtcctccag cgtgtcccag aatatctgca
      181 ggatgtagcg ctcggggctt tcgataccgc ggttcacctt gtagccccga aaccctttgg
      241 cctggctgat cacgtcgcgc aggccgcgcg tgatggcctc atcaaatgcg gcattctggc
      301 cggggtggat gcggatgtcg gcgagttcaa gaatcatgcg ggctccagtc ggtgatttcc
      361 acggggtgtt ctccccgtcc aatcgctgaa tgttaacggg gttggcgcat ctccactttt
      421 tacgccaaac gtttatcctt ggcggcatga agctgcataa ctacttccgc tcgtccgcct
      481 cgtttcgggt gcgtatcgcc ctggccctca agggcttgcc ctaccagtac attgcggtca
      541 atctggcgca gggtgaccac ctgctggacg cctaccgttc ccagtcgccc gaggtgctgg
      601 tgccattgct ggagatcgac ggccaccgcc tctcgcagtc catggccatc atcgaatacc
      661 tggacgaaac ccgcccggag ccgcctttgc tgcccgatga cccactgggc cgcgcgcacg
      721 tgcgcgcatt ggcgcaatcg gtggcctgcg agatccatcc actgaacaac ctgcgcgtgc
      781 tcaagtacct cagccgcgaa ctcaaggtgg acgaggacac caagaacacc tggtaccgcc
      841 actgggtccg cggggggctg gaggcattcg agcgtcaact gctggcgcag gatgccttgc
      901 gcagcgcagc cgggcagccg ccgtcggtct actgctgggg cgcatcgccc accatggcgg
      961 actgcctcct ggtgccccaa atcttcaacg cgcagcggtt ccatgtgaat ctggacgggc
     1021 tgccacgcac catgactgct tttgaggcct gcatgcagct gcaggccttc caggacgcgc
     1081 agccctccag ttgcccggat ttcgtcgcct agccatgggg ggcctgcatc tgcccgccgg
     1141 tgtggactgg ctggttcccc aatggccagc gccggcgcgg gtcaaggctg tgtgcaccac
     1201 ccgccagggt ggtaaatcgg aagcaccctg gaacagcctg aatctgggca cccacgtggg
     1261 ggacacgcct gacagcgtgc agcacaaccg cgccgtgctg cagaccgcga tgggtacaca
     1321 acttgtgttt ctcaaccagg tgcacgggtc gcacatggcg gccctgtcgc cggatacggc
     1381 cgatggttat gaagccgacg gtgcatggac cgaccagact ggtctggctt gtgtagtgat
     1441 ggtggcagat tgcctgccgg tgttgttgtg tgacacccag gggcgccggg tcgcagcggc
     1501 ccatgcgggc tggcgcgggc tggtgggcac ggatggcaaa ggcattctgg aggcatcttt
     1561 agagcctttt aggcctctag cccccgtgga atatgcgcag ggcgctactg aaataatagc
     1621 gtggttgggg ccgtgcattg gcccggaagc gtttgaagta ggcgacgaag ttcggacggt
     1681 atttgtggac gtgtcgcctg aggctgatca gtgttttcga ccgctgggtc agggtaaatg
     1741 gcttgcggac ctggctggtc tggcccgcca acgcctgcag gctctgggcg tgacaggcat
     1801 ctacggcaac gacagcacgg cggcgtggtg caccgtgact aacccctcac ggttcttttc
     1861 ccaccggcgg gaccgcgtca gtgggcggca ggccgcctgc atctggctgg agtgaagcca
     1921 gccgctcggc ggcttcacgc tcccggattc tgcgtttgcg tgccggggtg cccatgaagt
     1981 acagcattaa ccccacgggc agcatcccat acaacacaaa ggtgatgatc gctcccaata
     2041 cggatccgtt ggtgttggtg gcttctgctg cggccatcat gaaggcgacg taaatccagg
     2101 caatgggaat gatgtacatg gtgattttga ctaggggtat gtcaggatat cgtagggctt
     2161 gggctgagat acttcgagag taaactagat tcactaataa cgaggcgacg acgtgacgac
     2221 gcaaactccc aacgatttct ggacgcaggc gtcccagcag tttcagcagt ccatgtccga
     2281 gagctggggc aaggccatac agtcctttca gaccatggac atcggcagcg cgggagccag
     2341 tatgccttcc atgggaaaga agctgccaga gatccatttt gcccccgaga aactgcagga
     2401 attgcagcag caatacatgc aggaggccct tgccctgttc aacagcggca tgacgccacc
     2461 cgacaccaaa agtgacaagc gctttgccga tgccgcctgg ggggataacc cggtggccgc
     2521 ctatgccgca gcggtgtacc tgctcaatgc ccgcaccatg atgggcctgg cagaggcagt
     2581 ggatgccgat cccaagacgc gcaaccgcat ccgctttgcc atcgagcagt ggatggcggc
     2641 tgcagcgccg agcaacttca tggcgttcaa tgccgaagcc cagaaaaagg ccatcgagac
     2701 caagggcgag agcattgcca agggcatgaa gaacctgatg catgacctgc agcagggcca
     2761 tgtatccatg accgacgaga gcctgttcga agtaggcaag aacgtggcca cgaccgaagg
     2821 tgcggtggtg ttcgagaacg agctgttcca gcttatcgag tacaagccgc tcacggccaa
     2881 ggtttatgaa aagccttttt tgatggtgcc gccctgcatc aacaagttct acatcctgga
     2941 cctgcagccc gacaactcgc tggtgcgcta tgccgtggcg cagggccacc gcacctttgt
     3001 ggtgagctgg
//
LOCUS       DAOSJM010000311         3007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28504_length_3007_cov_4.057927, whole genome shotgun sequence.
ACCESSION   DAOSJM010000311 DAOSJM010000000
VERSION     DAOSJM010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28504_length_3007_cov_4.057927"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..1038
                     /locus_tag="PKX82_10405"
     CDS             211..1038
                     /locus_tag="PKX82_10405"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOF52710.1"
                     /translation="MTTSQTILITGCSSGIGQALALEFKKRGHTVFATARQPAALQAL
                     TAMGLHAVELDVTRADSIAQALAQIQAQAGRLDMLINNAGYGQFGAVIDTDPETLRRQ
                     FETNVVAPVALSRAFLPLLRASQQACIAHIGSISGLVTTPFAGVYCASKAALHALSDA
                     MRMELGPLGVRVVTVQPGGIQSRFGDNAEHQIEFPANSPYAAIARFIQGRAKLSQKGA
                     TPVDQFAATVVDHLLNPKAGPICRTGAQSTRLPLLKKLLPTRTLDAKMQKLFGLDQL"
     gene            complement(1065..>1748)
                     /gene="modA"
                     /locus_tag="PKX82_10410"
     CDS             complement(1065..>1748)
                     /gene="modA"
                     /locus_tag="PKX82_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOF52711.1"
                     /translation="TAIAKDYEARTGQKVVLTFGASGLLRERIEAGEGAQVFASADTD
                     HPQRLVRQGGWQEPKVFVRNSLCALTSDKLNATPATVLGTMLLPGVRLGTSTPKSDPS
                     GDYTWALFEKANGLQPGTYATLNAKAQKLAGATDSPKPPDSRGTYAWIMDTDQVDIFI
                     TYCTNAVAAQAEVPRLKVVQFPPALSVGAAYGLTVRQGAPAAASAFAAALLAPPAQAV
                     FSRLGFGAP"
     assembly_gap    1749..1848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1849..2661)
                     /locus_tag="PKX82_10415"
     CDS             complement(<1849..2661)
                     /locus_tag="PKX82_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOF52712.1"
                     /translation="MKCLKQFRALAPVQYAQSAIIFIAIGLASTATMAQSIVVASTTS
                     TEQSGLFAHLLPEFKKASGIDVKVVALGTGQAIDMGRRGDADVLFVHDQVAEEKVVAE
                     GFAVKRLEVMYNDFVLIGPKADPAKTQGKDIVEALKKVAGTNSAFVSRGDKSGTHAAE
                     LRFWKVAGLDDKTGSGYKSCGCGMGPALNMASATSAYVLADRGTWLNFKNRGDLAVLV
                     EGDKRLFNQYGVMLVNPARHPQTKAVEGQKFVDWVTSPVGQNVIASYKIGGEQ"
     gene            complement(2658..>3007)
                     /locus_tag="PKX82_10420"
     CDS             complement(2658..>3007)
                     /locus_tag="PKX82_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52713.1"
                     /translation="PARKLSGGQQQRVALARAWALSPQVLLLDEPTASLDPHAKREVE
                     ALIGDLARQGSGMTVLFASHNLGQVKRLATRVVYLEHGRVLADLPVQDFFGGTLLQTD
                     YPQAHLFVKGELA"
BASE COUNT          534 a          917 c          909 g          547 t
ORIGIN      
        1 aaccgcagcg ccagcgcaca ggagaagatc aaagtgcacg cggtgtgtgt ggccctgttc
       61 ctggtgtttg ggcacattgc catgatcgcc ggcatgctgg accccaccct gtttggctat
      121 gcaccggaca tgggcaatac catgccgatg gaccacgggc actgagcctg tgccctggtc
      181 ataatgggtc tgaaaaaaat tcaggccctt atgaccacct cgcaaaccat tctcatcacc
      241 ggttgctcca gcggcatcgg gcaagcactg gcattggaat tcaagaagcg cgggcacacc
      301 gtcttcgcca ccgcacgcca gccggctgcg ctgcaagccc tgacggcgat gggcttgcac
      361 gcggtggaac tcgatgtcac acgcgcggac agcatcgccc aggcgctggc gcagattcag
      421 gcgcaggcag gtcgactgga catgctcatc aacaatgctg gatacgggca gttcggcgcc
      481 gtgatcgata ccgacccgga gactttgcgc cgccagttcg agaccaacgt ggtggcgccg
      541 gtggcgctgt cccgcgcgtt cctgccgctg ctgcgcgcat cgcaacaggc gtgcatcgcc
      601 catatcggca gcatctcggg actggtcacg acgccctttg ctggcgtgta ttgcgccagc
      661 aaggcggcgc tgcatgcgct gtccgacgcc atgcgcatgg agctgggccc gctgggcgtg
      721 cgcgtggtga cagtacagcc cggtggcatc cagtccagat tcggtgacaa cgcggagcac
      781 cagattgaat tcccggccaa ttctccctat gcagcgattg ccaggttcat tcaagggcgg
      841 gcaaagctgt cccaaaaggg tgcgacaccg gttgaccagt tcgccgccac cgtggtggac
      901 catttgctta accccaaagc cggccccatc tgccgcacgg gcgcccaaag cacccgcttg
      961 ccattgctca aaaagctgct tcccacccgg acgctggacg cgaagatgca aaagctgttt
     1021 gggctcgacc agctctgaac cagtattcag agccgtgagt catttcaggg cgccccaaaa
     1081 cccaggcgac tgaacacagc ctgcgccggt ggcgcaagca gagcagcggc aaacgcgctg
     1141 gctgccgcgg gtgcgccctg gcgtaccgtc aaaccatagg ctgcgcccac cgagagtgct
     1201 ggcggaaact gcaccacttt cagacgcggc acttcggctt gcgcggctac ggcgttggtg
     1261 cagtaggtga tgaatatgtc cacctggtcg gtgtccatga tccaggcata ggtgccccgg
     1321 ctgtcgggcg gtttgggcga gtcggtggca ccggccagct tttgcgcctt ggcattcagc
     1381 gtggcgtagg tgccgggctg taagccattg gccttttcaa acagagccca cgtgtagtca
     1441 ccagagggat cggatttggg tgtggaggtg cccaggcgga cgccgggcag cagcatggtg
     1501 cccagcaccg tggccggggt ggcgttgagc ttgtcactgg tcagcgcaca cagggagttg
     1561 cgcacaaata cctttggctc ctgccagcca ccctggcgta ccaggcgctg cgggtggtcg
     1621 gtatcggccg aggcgaacac ctgcgcgccc tcgcccgcct cgatgcgctc gcgcagcagg
     1681 ccggaggcac cgaaagtgag cacgaccttc tggcccgtgc gggcctcgta gtctttggca
     1741 atagcggtnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct gttcgccacc
     1861 aatcttgtag ctggcgatca cgttttgccc tacgggcgag gtcacccagt ccacaaactt
     1921 ctggccttcc accgccttgg tttgcgggtg tctggcgggg ttgaccagca tgacgccgta
     1981 ctggttaaac aggcgcttgt cgccttcgac cagcaccgcc aggtcgcccc ggttcttgaa
     2041 attcagccag gtgccgcggt ctgccagcac ataggcactg gtggccgacg ccatgttcag
     2101 cgcagggccc atgccgcagc cgcaggactt gtagccactt cccgtcttgt catccaggcc
     2161 cgccactttc cagaagcgca gttcggcagc atgggtgccg ctcttgtcgc cacgcgacac
     2221 aaaggcgctg ttggtgccgg ccaccttttt gagtgcttcc acaatgtcct tgccctgggt
     2281 tttggccgga tcggccttgg ggccaatcag cacgaagtcg ttgtacatca cctccaggcg
     2341 tttgacggca aagccttcgg caacgacctt ttcctcggcg acctggtcgt gcacaaacag
     2401 tacatcggca tcgccgcggc ggcccatgtc gatggcctgg ccagtgccca gggcaacgac
     2461 tttcacgtcg atgccactgg ccttcttgaa ctccggcagc agatgggcaa acagcccgga
     2521 ctgttcggtg gacgtggtgg acgcgaccac aatggattgc gccatggtcg ctgtggaagc
     2581 cagtccgatt gctataaaaa taatagctga ctgcgcatat tggacggggg ctagagccct
     2641 gaattgcttc aaacacttca tgcaagttct cctttgacaa ataaatgggc ctgggggtaa
     2701 tcggtttgca gcagggtgcc gccgaagaaa tcctgcaccg gcaggtctgc cagcaccctg
     2761 ccatgctcca gatagaccac ccgggtggcc aggcgcttga cctgacccag gttgtggctg
     2821 gcaaacagca cggtcatgcc agagccctgc cgggccagat cgccgatcag cgcctcgact
     2881 tcgcgcttgg cgtgggggtc gaggctggcg gtgggctcat cgagcagcag cacctggggt
     2941 gacaaggccc atgcgcgggc caatgccaca cgctgctgct ggccgccgga cagcttgcgt
     3001 gccgggc
//
LOCUS       DAOSJM010000312         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_28754_length_2988_cov_3.625980, whole genome shotgun sequence.
ACCESSION   DAOSJM010000312 DAOSJM010000000
VERSION     DAOSJM010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28754_length_2988_cov_3.625980"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PKX82_10425"
     CDS             <1..592
                     /locus_tag="PKX82_10425"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52714.1"
                     /translation="DYQEAVQWYRRAADLGYALAQYGLGVAYQYGRGVQANPVVAYAL
                     YSLSSANDPSASNEATARREGLVPSLSLAEIAAAQALTRELTVAGTVTTGLNNFVASY
                     VPEKKAVKLAAVEKKPVAKTDAVVQATPPAPAPAAIDDGFPERPAQREGMLSCNTRCV
                     NAACWRTYDDGKKIKFRAQRKYNSATRQWELDSGGC"
     gene            594..1088
                     /locus_tag="PKX82_10430"
     CDS             594..1088
                     /locus_tag="PKX82_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF52715.1"
                     /translation="MIPTKNPNFFLRFATPDDAGLVVGFMKKLGSYQKMADKIIATEA
                     GIRKLLTEGKGESVFGIYEGEIVAFFYFCQTSSAFTGQSGLYMDGFYVDDAYRSHGFG
                     KVMMAFLSKLAIERGCQRLEWGCLDWNEPSINFYKSLGAYGVDNMTIYRFSPDTLREN
                     ALRF"
     gene            complement(1158..1233)
                     /locus_tag="PKX82_10435"
     tRNA            complement(1158..1233)
                     /locus_tag="PKX82_10435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1198..1200),aa:Thr,seq:cgt)
     gene            complement(1271..2281)
                     /locus_tag="PKX82_10440"
     CDS             complement(1271..2281)
                     /locus_tag="PKX82_10440"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOF52716.1"
                     /translation="MAKKISLTRYLVEQQRQEGHIPSQLRLLLEVVARACKSISQAVN
                     KGDLGDVMGTADSENVQGEIQKKLDIIANEVLIEANEWGGHLAGMASEEMEGIYVVPN
                     RYPQGEYLLMFDPLDGSSNIDVNVSIGTIFSVLRMPEDDRGVDEGDFLQAGAKQVAAG
                     YCVYGPQTTLVLTVGDGVAMFTLDREQGSFVLTRENVQIPADTKEFAINMSNMRHWDA
                     PVKRYVDECLQGKEGPRGKDFNMRWIASMVADVHRILTRGGIFMYPWDKREPNKPGKL
                     RLMYEANPMSWLIEQAGGAATNGKQRILDIQPTGLHERVSVILGSKNEVERVTRYHLE
                     AQ"
     gene            complement(2400..>2988)
                     /locus_tag="PKX82_10445"
     CDS             complement(2400..>2988)
                     /locus_tag="PKX82_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HOF52717.1"
                     /translation="AAVHNGDPLASAGPAAVWPGKTLQRFKDASNMTDRFNALQALVS
                     SGHPLATPALARFHQLFKDEALVLDKWFALQAGAPDRGGQVLPAVRQLMAHPDFHIKN
                     PNRARSVIFSYCSANPGGFHRTDAAGYVFWAERVIELDAINPQVAARLARAMDRWKRL
                     AEPYRSAAREALARVAAKTDLSNDVREVIDRALAD"
BASE COUNT          551 a          887 c          935 g          615 t
ORIGIN      
        1 ggactaccag gaggccgtgc agtggtaccg ccgcgcggca gacctgggct atgcgctggc
       61 ccagtatggt cttggggtgg cctaccaata cgggcgcggc gtgcaggcca acccggttgt
      121 tgcctatgcg ctctacagtc tgtccagcgc caatgaccct tccgccagca acgaggccac
      181 agcgcgccgc gaaggattgg ttccatccct atccctggca gagatcgcag ccgcgcaggc
      241 actgacccga gagctgacgg tggcaggcac cgtcaccacg gggttgaaca actttgttgc
      301 cagctatgtg ccagagaaaa aggccgtcaa gctagccgcc gtggagaaga aaccggtagc
      361 gaaaacggat gcagtcgttc aggcaacgcc accagccccc gcaccggctg ccatcgacga
      421 tggattcccc gagcgccccg cgcaacgcga aggcatgctg agttgcaata cgcgctgcgt
      481 gaatgcggct tgctggcgca cgtatgacga tggcaaaaaa atcaagttcc gggcgcaacg
      541 gaaatacaac tcggctacgc gacagtggga gctggattct ggggggtgct gaattgatcc
      601 ccacaaagaa ccccaacttc ttcctgcgct ttgctacgcc agacgacgcc ggattggtgg
      661 tcggttttat gaagaagctc ggcagctatc aaaagatggc cgacaagatc attgccaccg
      721 aagcaggtat tcgcaagctg ctcacggagg gtaagggcga gtcggttttc ggaatctacg
      781 aaggggaaat cgtcgccttc ttctacttct gccaaacctc ttcggctttt acaggccagt
      841 ccgggctcta catggacggt ttttatgtcg atgacgcgta ccgcagccat ggattcggaa
      901 aggtcatgat ggccttcctg tcgaagctgg cgatagaacg cggttgtcag cggctggaat
      961 ggggttgcct ggactggaac gagccctcca tcaacttcta caaaagtttg ggagcgtacg
     1021 gcgtagacaa catgacgatt taccgatttt ccccggacac attgagagag aacgcgcttc
     1081 gattttgatg tgacccggac ggacatacgc agcacattaa aaaacccgct gaccttgcga
     1141 tcaacgggtt ttaagagtgg tgccggagag atgaatcgaa cacccgacct tctcattacg
     1201 aatgagctgc tctaccgact gagctacacc ggcgtggtag cccgcaatta tagccagcgg
     1261 gcgtagcttc ctattgcgct tccaggtggt agcgggtgac gcgttccact tcgttcttcg
     1321 agcccagaat cacgctcacg cgctcgtgca gcccggtcgg ctggatgtcc aggatgcgct
     1381 gcttgccgtt ggtggctgcg ccgccagcct gctcgatcag ccagctcatg gggttggctt
     1441 cgtacatcag gcgcagcttg ccgggtttgt tgggttcgcg cttgtcccag gggtacatga
     1501 agatgccgcc gcgggtcagg atgcggtgca cgtcggccac catgctggca atccagcgca
     1561 tgttgaagtc cttgccgcgc gggccttcct tgccttgcag acattcgtcc acatagcgct
     1621 tcacgggcgc atcccagtga cgcatgttgc tcatgttgat ggcaaattcc ttggtgtcag
     1681 cgggaatctg cacgttctcg cgcgtcagca cgaacgagcc ctgctcgcgg tccagtgtga
     1741 acatggcgac gccatcgcct acggtcagca ccagcgtggt ttgcgggccg tagacgcagt
     1801 agcctgcagc cacctgtttg gcgccagcct gcaggaagtc gccttcgtcc acaccgcggt
     1861 cgtcttcggg catgcgcagc acgctgaaga tggtgccgat gctcacgttc acatcgatgt
     1921 tgctcgatcc gtccagcggg tcgaacatca gcaggtattc gccttgcggg taacggttgg
     1981 gcaccacgta gatgccttcc atttcctcac tggccatgcc agccaggtgg ccgccccatt
     2041 cgttggcttc gatcagcact tcgttggcaa tgatgtcgag ctttttctgg atttcgcctt
     2101 gcacgttctc gctgtcggcc gtgcccatca catcgcccag atcgcctttg ttcacggcct
     2161 ggctgatgct cttgcaggcg cgcgccacca cttcgagcag caggcgcagt tgcgagggaa
     2221 tatggccttc ctggcgctgc tgttcgacca ggtagcgggt aagagaaatt ttctttgcca
     2281 tgctttttcc tattttttag taaagataag acgttttccc gcagggccga cccaagggaa
     2341 aatgcggccc cctttggggg gcagtgaccc acacgtagtg agggaacgtg ggggccatgt
     2401 cagtcggcca gcgcgcggtc aatcacctcg cgcacgtcgt tgctcaggtc ggtcttggct
     2461 gccacgcggg ccagggcctc gcgtgctgcg ctgcggtagg gctcggccag gcgtttccag
     2521 cggtccatcg cgcgggccag ccgtgcggcc acctgtgggt tgatggcgtc gagctcgatc
     2581 acgcgctcgg cccagaacac atagcctgcc gcgtccgtgc ggtggaagcc gccggggttg
     2641 gcactgcagt agctgaagat gacgctgcgc gcgcggttgg ggttcttgat gtggaagtcc
     2701 gggtgggcca tgagctggcg cacggcaggc agcacctggc cgccacggtc gggtgcaccg
     2761 gcctgcaggg caaaccattt gtccagcacc agcgcctcgt ccttgaacag ctggtgaaag
     2821 cgtgccagcg cgggtgtggc cagcgggtgg ccactgctga ccagcgcctg cagggcgttg
     2881 aagcggtcgg tcatgttgct ggcgtccttg aagcgctgca gcgtcttgcc gggccagacc
     2941 gctgccggtc cggcgctggc caaggggtca ccgttgtgca cagcggcc
//
LOCUS       DAOSJM010000313         2961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_29064_length_2961_cov_5.322780, whole genome shotgun sequence.
ACCESSION   DAOSJM010000313 DAOSJM010000000
VERSION     DAOSJM010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29064_length_2961_cov_5.322780"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PKX82_10450"
     CDS             <1..1133
                     /locus_tag="PKX82_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOF52718.1"
                     /translation="PEDILREINRSVWTIGYTGQTPERLKAHMRNMHLFDVKTLRAKS
                     GVDKETGYDMSGDYFGLPWPCWGTGELKHPGSPNLYDTSKHVMDGGGNFRANFGVERD
                     GVSLLAEDGSHSLGADITTGYPEFDHVLLKKLGWWDELSEAEQKAAEGKNWKTDSSGG
                     IIRVVMKNHGCHPFGNAKARAVVWNFPDAIPQHREPLYGTRPDLVAKYPTHDDKKAFW
                     RLPTLYKTIQQKNVADKVHEKFPLILTSGRLVEYEGGGEETRSNPWLAELQQEAFVEI
                     NPTTAASRGIRNGERVWLNSPTGARLNVQALVTERVATDTVWMPFHFSGRWQGADMLA
                     YYPNGAAPVVRGEAVNTATTYGYDSVTMMQETKTTVCNIERA"
     gene            1161..1781
                     /gene="fdh3B"
                     /locus_tag="PKX82_10455"
     CDS             1161..1781
                     /gene="fdh3B"
                     /locus_tag="PKX82_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HOF52719.1"
                     /translation="MARMKFACDAERCIECNGCVTACKNEHEVPWGVNRRRVVTLNDG
                     VPGEKSISVACMHCSDAPCMAVCPVNCFYRTDEGVVLHDKDVCIGCGYCSYACPFGAP
                     QFPAAGTFGVRGKMDKCTFCAGGPEANGSQAEFEKYGRNRLSEGKLPACAEMCSTKAL
                     LAGDGDVVADIFRTRVVQRGKGAEVWGWGTAYGSKKAGQAPEGAKS"
     gene            1781..2008
                     /locus_tag="PKX82_10460"
     CDS             1781..2008
                     /locus_tag="PKX82_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52720.1"
                     /translation="MTTVLRIIGVVAVVGLAACGEKPQTLGAGKTDVSPYQGGESAFA
                     TPGWKAGDKTSWEQAMKARALNTQNEYNRTR"
     gene            2020..>2961
                     /locus_tag="PKX82_10465"
     CDS             2020..>2961
                     /locus_tag="PKX82_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOF52721.1"
                     /translation="MQRARLALFFVAFCWGGLALAQSAAAPAAPATTAGVQSQDIRDV
                     APEASAAPGYAQQTNGERAQVQPGNNAPMWRQVGSGVTGYSSLPTSQAPEAGNLIQPF
                     VQYPGSRLTTSGEAWRQVRNNWIIPYGAALIAISALALAMLYWKKGSLGKNHPENGPQ
                     IERFTPFERAAHWSNAAAFLALAISGLVMAYGKFFLLPVIGSTLFGWLTFALKNLHNF
                     TGPLFAVSLCVIIITFIKDNIANAADFVWLSKAGGMLGDQEVPSHRFNAGEKGLFWWG
                     VTIPGVVVVVTGLILDKLIPGLGILRGDMQVAHMIHSV"
BASE COUNT          611 a          838 c          923 g          589 t
ORIGIN      
        1 tgccggaaga catcctgcgc gagatcaacc gctcggtctg gaccattggc tacaccggac
       61 agacgccgga gcggctcaaa gcgcacatgc gcaacatgca cctgtttgac gtcaaaaccc
      121 tgcgtgccaa gtccggtgtg gacaaggaaa ccggatatga catgagtggc gattatttcg
      181 gtctgccctg gccttgctgg ggtacaggag agctcaagca cccgggttcc cccaacctgt
      241 acgacacctc caagcacgtc atggacggtg gtggcaactt ccgggccaat tttggcgtgg
      301 agcgcgacgg tgtttccctg ctggcagaag acggctccca ctctttgggc gccgacatca
      361 ccacgggcta ccccgagttc gaccatgtgc tgctgaagaa gctgggctgg tgggacgaac
      421 tgagcgaggc tgagcagaag gcggctgagg gtaagaactg gaagaccgat tcgtcgggcg
      481 gcatcatccg tgtggtgatg aaaaaccatg gttgccatcc ctttggcaat gccaaggccc
      541 gcgcagtggt gtggaacttt ccggatgcca ttccccagca ccgtgagccc ctgtacggca
      601 cacgtccgga tctggtggcc aagtacccca cccacgacga caaaaaagcg ttctggcgtt
      661 tgcccacact gtacaaaacc attcagcaaa agaacgtggc cgacaaggtc cacgaaaagt
      721 tcccgctgat cctgacctca ggccggctgg tggagtacga ggggggcggt gaagaaaccc
      781 gatccaatcc ctggctggcc gagttgcagc aggaagcctt tgtcgagatc aatccgacaa
      841 cagctgcatc gcgtggcatc cgcaatggag agcgtgtctg gctgaattca ccaaccggtg
      901 cacgactcaa tgtccaggca ctggtaaccg agcgtgtggc gaccgatacg gtgtggatgc
      961 cgttccactt ttctggtcgc tggcagggag ccgatatgct cgcttactac cccaatggcg
     1021 ctgcgccagt agtgcgcggt gaggcagtga atacagcaac cacctatggc tatgacagtg
     1081 tgacgatgat gcaggagacc aagacaacgg tctgcaacat cgagcgcgct tgacccccta
     1141 gctaggacca ggagacagat atggcaagaa tgaaattcgc atgcgatgcc gagcggtgta
     1201 tcgagtgcaa tggctgcgtg acagcatgca agaacgagca tgaagttcct tggggtgtca
     1261 atcgccggcg tgtggtgacc ctcaatgacg gtgtgccagg agaaaaatcc atttctgtgg
     1321 cgtgcatgca ctgcagtgat gcaccctgca tggctgtgtg cccggtgaac tgcttctacc
     1381 gtaccgatga aggcgtggtg ctgcatgaca aggacgtctg catcggttgc ggctactgct
     1441 cctacgcctg cccgtttggt gctccgcaat tccccgcagc tggcactttc ggcgtgcgcg
     1501 gcaagatgga taaatgcacc ttctgtgccg gtggccccga ggccaatggc agccaggccg
     1561 agttcgagaa gtacggacgc aaccgtctgt ccgaaggcaa gctgcccgcc tgcgcagaaa
     1621 tgtgttccac caaggcgctg ctggccggtg atggcgatgt agtcgccgat atcttccgta
     1681 cccgcgtggt gcagcgtggc aagggtgctg aggtctgggg ctggggtacc gcctacggat
     1741 ccaagaaggc aggccaggcc cctgaaggag ccaaatcatg atgactactg tgttgcgcat
     1801 catcggcgtt gtggctgttg tggggctggc ggcgtgcggc gagaagccgc aaaccctggg
     1861 ggcgggcaag accgacgtgt ctccctacca gggcggagaa agcgcgtttg caacgccagg
     1921 ctggaaagcc ggtgacaaga cgagctggga acaggccatg aaagcccgtg ccctgaatac
     1981 gcagaacgaa tacaacagaa ccaggtgatc ggagccctta tgcaacgcgc acggttagct
     2041 ctgttttttg tagcattctg ttggggtggg cttgccctgg cccagagcgc tgcagcgcct
     2101 gcggcaccag cgacaaccgc aggtgtgcag tcgcaagaca ttcgtgatgt tgcgcccgag
     2161 gccagcgcgg cgccaggcta tgcgcagcaa accaatggcg agcgggccca ggtgcagcct
     2221 gggaataatg cgcccatgtg gcgccaggtg ggcagtgggg tgaccggcta cagcagcctg
     2281 cccacatcgc aggccccgga ggctggtaac ctgattcagc cttttgtgca atatcccgga
     2341 tcgcgcctga ccactagtgg cgaggcttgg cggcaggtgc gcaacaactg gatcattccc
     2401 tacggagcag cgctgattgc catttcggct ctggcgctcg ccatgctgta ctggaagaag
     2461 ggctcgctgg gcaagaacca ccctgaaaat ggccctcaga tcgagcgctt caccccattc
     2521 gaacgtgcgg cccactggtc caatgcggcc gcttttctgg ccttggcaat ctccggtctg
     2581 gtcatggctt acggcaagtt tttcctgctg cccgtgattg gcagcaccct gtttggctgg
     2641 ctgactttcg cactcaagaa cctgcacaac ttcaccggac cgctttttgc tgtgtcgctg
     2701 tgcgtgatca tcatcacctt catcaaggac aacatcgcca atgcggcaga ttttgtctgg
     2761 ctttccaaag ccggtggcat gctgggtgat caggaagtgc cttcgcaccg cttcaatgcg
     2821 ggtgaaaaag gcctgttctg gtggggtgtg accattcccg gcgtggtagt ggtcgttacg
     2881 ggattgattc tcgacaagct gattcccggt ctgggcattt tgcgtggcga catgcaggtt
     2941 gcccacatga ttcattcggt g
//
LOCUS       DAOSJM010000314         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_29194_length_2951_cov_5.071823, whole genome shotgun sequence.
ACCESSION   DAOSJM010000314 DAOSJM010000000
VERSION     DAOSJM010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29194_length_2951_cov_5.071823"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="PKX82_10470"
     CDS             <1..1528
                     /locus_tag="PKX82_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOF52722.1"
                     /translation="IDKAALGGGEAARAKHTARGKLLPRDRVGMLLDPGTPFLELSPL
                     AALAMYPDRDGSDSAPCAGVIAGIGRVSGVDCMIVCNDATVKGGTYYPLTVKKHLRAQ
                     EVAAQNNLPCIYLVDSGGANLPNQDEVFPDRDHFGRIFFNQANMSAQGIAQIAVVMGS
                     CTAGGAYVPAMSDETIIVKNQGTIFLGGPPLVKAATGEVVTAEDLGGGDVHTRLSGVA
                     DHLAQNDLHALSLAREAISHLNKNKVLSPITQAPVAPKFAADELYGVIPTDTRKPFDV
                     REIIARIVDASEFHEFKPRYGNTLVCGFAHIEGMPVGIIANNGILFSESALKGAHFIE
                     LCCQRKIPLVFLQNITGFMVGRKYENEGIARNGAKMVTAVATAQVPKFTIIIGGSFGA
                     GNYGMCGRAYSPRFLWMWPNARISVMGGEQAASVLATVKRDGIEAKGGAWSKDEEESF
                     KAPIRQQYEVQGHPYYATARLWDDGIIDPADTRRVLALGLSASLNAPIPDTKFGVFRM
                     "
     gene            1549..2220
                     /locus_tag="PKX82_10475"
     CDS             1549..2220
                     /locus_tag="PKX82_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOF52723.1"
                     /translation="MQSLDTAQLIALAGALGWASGVRLYLVVLLTGFAGFMGWVELPL
                     GLRLLAHPVVLGASGFMVLVEFFADKVPGLDSLWDMVHTVIRIPAGAALAAGVFGADS
                     AAMGLVAAVLGGSLAATSHAAKTTTRAAINTSPEPFTNIGASLAEDGLVPLALWLSVT
                     HPMAFAVLLVVLLVLGVWLIRNCWRYLRSLFSRLTRIFSGMPDPGALPAFRFSKNSPG
                     EPPHV"
     gene            2213..>2951
                     /locus_tag="PKX82_10480"
     CDS             2213..>2951
                     /locus_tag="PKX82_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOF52724.1"
                     /translation="MFNKILIANRGEIACRVAATAARLAIKTVAVYSDADATSKHVAA
                     CDEAVYIGGSSPKDSYLRWEKIIAAALETGAQAIHPGYGFLSENEEFAQACANAGLVF
                     IGPPPSAIKDMGLKAESKQLMEKAGVPLVPGYHGANQDPALLQAEADRIGYPVLIKAS
                     AGGGGKGMRAVDKSEDFAAALASCKREAINSFGDDAVLIEKYVQRPRHIEIQVFGDTQ
                     GNYVYLFERDCSVQRRHQKVLEEAPAPG"
BASE COUNT          524 a          952 c          930 g          545 t
ORIGIN      
        1 aatcgacaag gcggcactcg gtggtggcga ggcagcgcgc gccaagcaca ccgcccgcgg
       61 caagctgctg ccgcgcgacc gcgtgggcat gctgctggac cccggcaccc cgttcctcga
      121 actctcgccg ctggccgccc tggccatgta cccggaccgt gacggcagcg acagtgcacc
      181 gtgcgcgggc gtgattgccg gtatcggccg cgtcagtggg gtggattgca tgatcgtctg
      241 caatgacgcc accgtgaagg gcggtaccta ctacccgctc accgtcaaga agcacctgcg
      301 cgcgcaggaa gtggcggcgc agaacaacct gccctgtatt tacctggtgg actcgggcgg
      361 cgccaacctg ccgaaccagg acgaggtctt ccccgaccgc gaccactttg gccgtatctt
      421 cttcaaccag gccaatatga gcgcgcaggg cattgcccag atcgctgtgg tcatgggttc
      481 ttgcaccgcc ggtggcgcct atgtgccggc catgagcgac gaaactatca tcgtcaagaa
      541 ccagggcacc atcttcctgg gcggtcctcc gctggtgaaa gcggccacgg gcgaagtggt
      601 gacggccgaa gacctggggg ggggcgatgt gcatacgcgc ctctccggcg tggccgacca
      661 tctggcgcaa aacgatttgc atgccctgtc gctggcgcgc gaggcgattt cgcatttgaa
      721 caaaaacaag gtgctgtccc ccatcacaca agcgccggtt gctcctaaat ttgcagcaga
      781 cgagttgtat ggcgtgattc ccaccgacac ccgcaagccg tttgatgtgc gcgaaatcat
      841 tgcccgcatc gtcgatgcgt ccgagttcca tgagttcaag ccacgctatg gcaacacgct
      901 ggtgtgcggc tttgcccaca tcgaaggcat gccggtgggc atcattgcca acaacggcat
      961 tctgttcagt gagtcggcgc tcaagggcgc gcacttcatc gaactgtgct gccagcgcaa
     1021 gattccgctg gtgttcctgc agaacatcac cggcttcatg gtcgggcgca aatacgagaa
     1081 cgaaggcatt gcccgcaatg gcgccaagat ggtgaccgcc gtggccaccg cccaggtgcc
     1141 caagttcacc atcatcatcg gcggcagctt tggcgccggc aactacggca tgtgtggccg
     1201 cgcctacagc ccgcgcttcc tgtggatgtg gcccaacgcc cgcatctcgg tgatgggcgg
     1261 cgagcaggcg gccagtgtgc tggccactgt caagcgcgac ggcattgagg ccaagggcgg
     1321 cgcctggagc aaggacgagg aagagtcgtt caaggcgccg atccgccagc agtacgaagt
     1381 gcagggccac ccctactacg ccaccgcgcg cctgtgggac gacggcatca ttgaccccgc
     1441 cgacacccgc cgcgtactgg cgctgggact gagtgcgtcg ctgaatgctc ccattcccga
     1501 taccaagttc ggcgtgttcc ggatgtaagg gcacgcagga ctgcagccat gcaaagcctc
     1561 gatacggccc aactcatcgc cctggcaggt gccctgggct gggccagtgg cgtgcgcctg
     1621 tacttggtgg tgttgctcac cgggtttgct ggcttcatgg gctgggtcga gctgcccctg
     1681 gggctgcgcc tgctggccca cccggtggtg ctgggcgcca gcgggttcat ggtgctcgtc
     1741 gagttttttg ccgacaaggt tcctggcctg gactcactct gggacatggt gcacacggtg
     1801 atccgcattc ccgccggcgc agcactggcg gccggtgtgt ttggtgccga cagtgcggcc
     1861 atggggctgg tggctgccgt gttgggcggc tcgctggctg caaccagcca tgccgccaaa
     1921 accaccaccc gcgcggccat caatacctcg ccggagcctt ttaccaacat cggtgcatcg
     1981 ctggccgagg atggtctggt gcccctggcg ctgtggctgt cggtcaccca cccgatggcc
     2041 tttgcggttc tgctggtcgt tctgctggtg ctgggtgtct ggctgatccg taactgctgg
     2101 cgctacctgc gttcactctt ttcgcgttta acccgcattt tcagcggcat gcccgacccc
     2161 ggtgccttgc ccgcattccg cttttccaag aattcccccg gagaacctcc ccatgtttaa
     2221 caagatcctt atcgccaacc gtggcgaaat cgcctgccgc gtggctgcga cggccgcgcg
     2281 cctggccatc aagacggtgg ccgtgtattc cgatgccgat gccacgtcca agcacgttgc
     2341 cgcctgcgac gaggccgtgt acatcggtgg cagctccccc aaggacagtt acctgcgttg
     2401 ggaaaaaatc atcgcggccg cgctggaaac aggtgcacag gccatccacc ccggctacgg
     2461 attcctgagc gaaaacgaag aatttgccca ggcgtgcgcc aacgccggcc tcgtattcat
     2521 tggcccgcca ccgtcggcca tcaaggacat gggcttgaaa gccgaatcca aacaactgat
     2581 ggaaaaagct ggcgtgccgc tggtgcccgg ctaccacggt gccaaccagg atccggcatt
     2641 gttgcaggct gaggccgacc gtattggcta ccccgtgctg atcaaggcca gcgccggcgg
     2701 cggtggcaag ggcatgcgtg cggtggacaa gtccgaggac tttgccgccg cgctggcctc
     2761 gtgcaagcgc gaagccatca atagctttgg cgacgacgcg gtgctgatcg aaaaatacgt
     2821 gcagcgccca cgccatatcg agatccaggt gtttggcgac acgcagggca actatgtgta
     2881 cctgttcgag cgcgattgct cggtgcagcg ccgccaccag aaggtgctgg aagaagcccc
     2941 cgcccccggc a
//
LOCUS       DAOSJM010000315         2946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_29265_length_2946_cov_3.855067, whole genome shotgun sequence.
ACCESSION   DAOSJM010000315 DAOSJM010000000
VERSION     DAOSJM010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29265_length_2946_cov_3.855067"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..171
                     /locus_tag="PKX82_10485"
     CDS             <1..171
                     /locus_tag="PKX82_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOF52725.1"
                     /translation="RVAHVGSSSVRYEVGIFGDDELCAAKGHFVHVYVDAASRRPTAL
                     PEKLKTVLETLR"
     gene            168..863
                     /locus_tag="PKX82_10490"
     CDS             168..863
                     /locus_tag="PKX82_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOF52726.1"
                     /translation="MSFKEVDSAITSRMSVRAFTQQPVSRSDLEDILQVASRAASGTN
                     TQPWHVYVLQGKSRDTLVEKVCAVHDAIRAKPELAANYVEEYDYYPTQWISPFIDRRR
                     ENGWSLYGLLGIGKGDKDKMHLQHQRNYKFFDAPVGLMFTVDRAMGRGSLLDFGGFMQ
                     NIMVAARARGLDTCPQAAWNGFAKIVMPHIGAGDNEMLVCGMALGYADKSDVVNTFHT
                     PRVPVAEFTKWLD"
     gene            871..2712
                     /locus_tag="PKX82_10495"
     CDS             871..2712
                     /locus_tag="PKX82_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF52727.1"
                     /translation="MAFAAIQFLNGLSYASTLFLMAAGLTLIFGVTRIVNFAHGSFFM
                     LGALLSAHWLVDWFPAWGDSAWLYLAAMLLGAICAGLAGAMAEFVLLRRLYGVPELYQ
                     LVATFGLSLAMHDAMRWAFGPDEVFAPRFPGLKGALEFGGEFFPQYQAVTIVLGPLVW
                     LGLHLLLHHTGFGARLRAATQDRTMLSALGVNPRPLMLGAVVLGCALAGLGGALQLPR
                     EPAHLQMDVNIVVETFVVVVTGGLGSIGGAFLAALLIGLVHAFGTSLLPEATLVLVFA
                     TMGVVLAVRPQGLLGQPLEPVAQELGHRFAWGPRSGVVFTGVCAAVALLLAAVWWAGD
                     YAQSLASDALILLVLGVSLQAMMALGGLVSFGHAAFFALGAYGAALSHSVWGWSLPGA
                     LAAGCALSLLVAAVLGGVMVRSAGVYLAMLSLALAQVVWATASQWVNLTGGDNGLIGL
                     QLVTPDTRPVFFALLVLVALAGVAALRRLSRSTLGAALQAVRDAPQRAAASGLQAHGI
                     KYRIFVESATLAGLAGGLFAAHKGAVFPSVASVGTSVDALLVILLGGLHQVWGAAAGA
                     AILVGVAGELGRGFEYWRGALGVLVMAIMVAAPSGLMGLRLWTRRRA"
     gene            2709..>2946
                     /locus_tag="PKX82_10500"
     CDS             2709..>2946
                     /locus_tag="PKX82_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52728.1"
                     /translation="MTLEVRSLVKHFGGVQAVRGVSFGVAAGECVALIGPNGAGKSTT
                     FACIAGQHALTQGEVSWNGMRVDGLSAVQRLRQGI"
BASE COUNT          412 a          846 c         1053 g          635 t
ORIGIN      
        1 cgtgtggccc atgtaggatc atcgagtgtg cgttacgagg tgggtatttt tggtgacgat
       61 gagttgtgcg ccgccaaagg ccacttcgtg cacgtgtatg tggatgccgc cagccggcgc
      121 cccacagcct tgcctgaaaa actgaaaact gttctggaga ccttgcgatg agtttcaaag
      181 aggtggattc cgccattacg tcgcgcatgt ccgtgcgcgc ttttacccag caaccggtgt
      241 ccaggagcga cctggaagac attttgcagg tggccagccg cgctgcgtcg ggcaccaaca
      301 cccagccctg gcatgtctac gtattgcagg gcaagagccg cgacacgctg gttgaaaagg
      361 tctgtgccgt ccatgacgct atccgtgcca aaccggaact ggcggccaac tacgtggaag
      421 agtatgacta ttaccccacc caatggatca gcccgttcat tgaccgccgc cgtgaaaatg
      481 gctggagcct gtacgggcta ctgggcattg gcaagggcga caaggacaag atgcacttgc
      541 agcaccagcg caactacaag ttcttcgatg cgcccgtggg cctgatgttc accgtggacc
      601 gcgccatggg gcgtggctcg ctgctggatt ttggtggctt catgcagaac atcatggtcg
      661 ctgcccgtgc ccgcgggctg gacacctgcc cacaggccgc ctggaacgga ttcgccaaga
      721 tcgtgatgcc gcacatcgga gctggtgaca acgaaatgct ggtctgtggc atggcgttgg
      781 gctatgccga caagtcggac gtggtcaaca ccttccacac gccgcgcgtg ccggtcgcag
      841 agttcacgaa gtggctcgac tgagctgctg atggccttcg ccgcgatcca gttcctcaac
      901 gggctgagtt acgccagtac cctgttcctg atggcagcgg ggctcaccct gatcttcggg
      961 gtgacccgca tcgtcaattt tgcgcacggc agtttcttca tgctgggcgc gttgctgtcg
     1021 gcgcactggc tggtggactg gttcccggcc tggggtgaca gtgcgtggct gtacctggct
     1081 gctatgcttt taggagctat ctgcgcagga ctggcggggg ctatggccga atttgtcttg
     1141 ttgcgcaggc tctatggcgt accggagctg taccagctgg tggcaacctt tggcctgagt
     1201 ctggccatgc acgacgccat gcgctgggcg ttcgggcccg acgaagtatt tgccccgcgc
     1261 tttccgggac tcaagggcgc gttggaattc ggcggcgaat tttttcccca ataccaggca
     1321 gtcaccatcg tgctcgggcc gctggtgtgg ctggggctgc atctgctgct ccaccacacc
     1381 ggctttggcg cgcgcttgcg cgcggcaacc caggaccgaa ccatgctgtc ggccctgggc
     1441 gtcaaccccc ggccgctgat gctgggtgcg gtggtgctgg gctgtgcgct ggctgggctg
     1501 ggcggggcgc tgcaactgcc gcgcgagcct gcccatctgc agatggatgt gaatatcgtg
     1561 gtggaaacct ttgtcgtggt ggtcactggt ggtctgggca gtattggcgg cgcgtttctc
     1621 gctgcgttgc tgatcgggct ggtccatgcc tttggcacca gcctgttgcc cgaagccacg
     1681 ctggtgctgg tatttgccac catgggtgtg gtgctggcgg tgcggcccca ggggctgctg
     1741 ggccagccgc tggaacccgt ggcgcaggag ctggggcacc gctttgcctg ggggccgcgt
     1801 tccggcgtcg tcttcacagg tgtctgcgcg gccgttgcgt tgctgctggc agctgtctgg
     1861 tgggctggtg actacgccca gtcgctggcc agcgatgcct tgattttgtt ggtgctgggc
     1921 gtgagcctgc aggccatgat ggcgctgggt ggcttggtga gtttcggcca cgcagcgttc
     1981 tttgcgctgg gggcgtacgg tgcggcgctg tcgcactccg tgtggggctg gagcctgccc
     2041 ggcgcactgg cagcgggctg tgcgctcagt ctgctggtgg ccgcggttct gggtggggtg
     2101 atggtgcgca gcgccggggt gtatctggcc atgctgtcgc tggcactggc gcaagtggtc
     2161 tgggcgactg ccagccagtg ggtgaacctg acagggggtg acaatggcct tattggtctg
     2221 cagctggtca cgcccgacac gcggcccgtc ttctttgccc tgctggtgct ggtggcgctg
     2281 gccggcgtgg ccgctttgcg tcgcctgagc cgctctactt tgggggctgc attgcaggcc
     2341 gtgcgcgacg caccgcagcg tgccgccgcc agcggcttgc aggcgcatgg catcaagtac
     2401 cggatattcg tggagagcgc caccctggcg gggctggcgg gtggcttgtt tgcggcacat
     2461 aagggggcgg tgtttccgtc ggtagcctcg gttggcacct cggtcgatgc actgctggtc
     2521 atcctgctcg gcggcttgca ccaggtgtgg ggtgcggcgg caggggcagc cattctggtg
     2581 ggtgtggcgg gggaactggg gcgcggtttt gagtactggc gtggtgcgct cggtgtgctg
     2641 gtcatggcca tcatggtggc ggcgccgtcc ggcctgatgg gcttgcggct gtggacccgg
     2701 aggcgcgcat gacgctggaa gtacgcagtc tcgtcaagca ctttggcggc gtacaggccg
     2761 tccgcggcgt gtcgttcggc gtagcagccg gagagtgcgt cgcattgatc ggccccaatg
     2821 gcgccggcaa gtccaccacc tttgcctgta tcgccggtca acacgcgctg acgcagggcg
     2881 aggtatcctg gaacggcatg cgggtggacg ggctttctgc ggtgcagcgc ttgcggcagg
     2941 gcatag
//
LOCUS       DAOSJM010000316         2943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_29300_length_2943_cov_4.784626, whole genome shotgun sequence.
ACCESSION   DAOSJM010000316 DAOSJM010000000
VERSION     DAOSJM010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29300_length_2943_cov_4.784626"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="PKX82_10505"
     CDS             complement(<1..560)
                     /locus_tag="PKX82_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOF52729.1"
                     /translation="MLRTHRRACNLCEAICGLTVEIDGERIVSIKGDVNDPHSKGFVC
                     PKALALKDLYEDPYRLRQPMRRTAHGWVPVSWEDALDEAGRRIAEVQKRDGKDALGIY
                     WGNPPTHNHGLVLTMNPVFDALGTRNRFSAASADQLPQYLVSRQMFGNQLMFPVPDVD
                     RTQFWLILGGNPLASNGSVGSGPDMRG"
     gene            974..1555
                     /locus_tag="PKX82_10510"
     CDS             974..1555
                     /locus_tag="PKX82_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52730.1"
                     /translation="MSHWYFASVLSAIFFSASALALTPNEIASIKPGTQLELPVVDTD
                     TPALETIDIHTVATITDPDGTLWYRFNGKRRDQSAAAIIAHIENGQVEVVSSIRLLKL
                     RDIGANPKQVWRMDKLAQGELNFENQPYKFNADESDDAKYVDTSGTQTSISYYKFENA
                     TDDDLSLMVFEWAEDNFEVLALEWVDSEKARIK"
     gene            1552..2601
                     /locus_tag="PKX82_10515"
     CDS             1552..2601
                     /locus_tag="PKX82_10515"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOF52731.1"
                     /translation="MNARGPHALLPELRPGAFYVATEGEPPGPVTYCEDTSDRYDIFG
                     FRCRAVVCSVQTAFQNVLIADTVSYGRALFLDGVLQSSEFDEALYHELLVQPAMCIHP
                     NPQEVLIIGGGEGATLREVLAHRTVKRAVMVDIDETCVDLCREHLRDWHQGAMDDPRA
                     QLVYVDGRAFVEEDDRFYDVVIIDVVDMLENGPAQRIYTREFYAHLRNRLRPGGIVVV
                     QGMEFSHVDHVQHAALVRTLRTNFSEVHSYQSHIPSFVSPWGFVVASDHVRPYEYSAA
                     SIDAQIQRKLGTEWMQHVDGNFMLSRFELCAATRRMLSMPGPTLGDDLSFVKPADIEF
                     VSEPIAVFPSRPTAP"
     gene            2604..>2943
                     /locus_tag="PKX82_10520"
     CDS             2604..>2943
                     /locus_tag="PKX82_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52732.1"
                     /translation="MLQYTYEIQRPVANTPGNRRNLFGYEATLQQATEVQPEQTWPDK
                     EHVILVQQLLLDTRAELDAEMHAWVDSASAGQKLEICFRSIRVVWKSGRAFVQAPDGH
                     VQAALDAILDY"
BASE COUNT          652 a          831 c          822 g          638 t
ORIGIN      
        1 ccgcgcatgt ccggccccga gcccacgctt ccattggacg ccaacgggtt gccgcccaga
       61 atcagccaga actgcgtgcg gtccacatcg ggcacgggaa acatcagctg gttgccaaac
      121 atctggcggc tcaccaggta ctgcggcaac tggtcggccg atgcggcaga aaaccggttg
      181 cgcgtgccca acgcatcaaa caccgggttc atggtcagca ccaggccgtg gttgtgcgta
      241 ggcgggttgc cccagtagat gcccagtgcg tccttgccat cgcgcttctg gacttcggcg
      301 atgcgccggc ccgcttcgtc cagtgcatcc tcccacgaca ccggcaccca gccgtgggcc
      361 gtgcgacgca tgggctggcg caggcggtag gggtcttcgt acaggtcctt gagcgccagc
      421 gccttggggc acacaaaacc cttgctgtgc gggtcattca cgtcgccctt gatggaaaca
      481 atgcgctcac catcgatctc caccgtcagg ccacagatgg cttcgcacag gttgcaggca
      541 cggcggtggg tacggagcat ggcaggttcc tcgggaagca tgggcggcaa gggcttcaag
      601 actttttggc cccttccccc cgtggaatat gcgcaatgcg ctctgttttt tgtagcgtat
      661 ttacccttga cacacacaac cacgttagcg tgctgacgtg gcaagtggct attccattac
      721 cttgatctgg ttgagaatac atcgagaatc gtccattgcg tggttgaatt tcaaccacca
      781 aaatccaaca acgattgctt gacgcggatt cagtagtacg tgcatcttgc caaggcgctt
      841 gcggtacagg cgcctcaccc attcacacac gctgatctct gcaagcaaga gtcagttctt
      901 ggaaacagct gcgtgtatat tcaatatcaa gttgttccag agttttttta accgccaact
      961 cacggagaaa tgcatgtctc actggtattt tgctagcgtt ctcagcgcaa tatttttttc
     1021 tgcttcggcc cttgctttga cgcccaatga aattgcaagc atcaaacccg gaacgcaact
     1081 cgaactgcct gttgtagaca ccgacacgcc ggcactggaa acaatcgaca tacacacggt
     1141 agccaccatc actgatcctg acgggactct gtggtaccgg ttcaacggca agcgccgtga
     1201 tcagtccgcc gcagccatca ttgctcacat tgaaaacggg caagtcgaag tggtatccag
     1261 cattcgcctt ttgaagctgc gcgacattgg cgcaaatccc aagcaggttt ggagaatgga
     1321 caaactggcg cagggcgaat tgaactttga aaatcagccc tataaattca acgccgacga
     1381 atcggatgat gccaaatacg tagacacaag tggcacgcaa accagcatca gctattacaa
     1441 atttgagaac gcaaccgacg acgacctgtc gctgatggtg tttgaatggg cggaagacaa
     1501 ctttgaagtg ctcgcgctgg aatgggtgga ttctgaaaaa gccagaatta aatgaatgca
     1561 cgtggcccgc acgcattgct gcccgagctc agacccggag cgttttatgt cgccactgag
     1621 ggcgagcctc cgggtccggt gacctactgc gaagacacct cggatcggta cgacatattt
     1681 ggtttccggt gcagggcggt tgtctgctcg gtacagaccg catttcagaa cgttctgatt
     1741 gccgataccg tgagctacgg gcgggccttg tttctggacg gcgtccttca aagcagcgaa
     1801 ttcgacgaag cgctgtacca cgaactgctg gtgcaacccg ccatgtgcat ccatcccaac
     1861 ccccaagagg tactgattat tgggggcgga gagggcgcta cattgcgcga ggtcctggcg
     1921 caccggacgg tgaaacgtgc cgttatggtg gatatcgacg agacctgtgt tgatctctgc
     1981 agggagcact tgcgtgactg gcaccaaggt gcaatggatg acccgcgtgc acaactggtg
     2041 tacgtcgatg ggagggcttt cgtcgaagag gacgaccgtt tttacgatgt agtgatcatt
     2101 gatgtcgtgg acatgctgga gaacgggccg gcgcagcgta tctacacacg ggagttctat
     2161 gcccacttgc gcaatcgatt gcgtcctggg ggcatcgttg tcgttcaagg catggagttt
     2221 tcgcacgttg accatgtcca gcatgcggca ctggtgcgta ctttgcgaac gaatttttcg
     2281 gaagtgcata gttaccaatc gcacatccca tccttcgtat ccccctgggg gtttgtcgtg
     2341 gcatcagacc acgttcgccc ttatgagtat tccgcagcgt ccatcgacgc gcaaatccag
     2401 cgcaagctgg gcacggaatg gatgcaacat gtagacggca actttatgct ttcacggttt
     2461 gaactgtgcg cagccacacg ccggatgctc tcgatgccag gccccacgct gggcgatgac
     2521 ctttcatttg tcaagcccgc ggatatcgaa tttgtcagtg aacccatcgc tgtattcccc
     2581 agtcgcccca cggcaccata agcatgttgc aatacaccta cgaaatccag cgtcccgtcg
     2641 caaacacccc ggggaatcgt cgcaacctgt tcgggtatga agccacactg cagcaagcta
     2701 cggaggttca gccagaacaa acttggcctg acaaagagca tgtcattctt gtgcagcagt
     2761 tgctgttgga tacccgcgcg gaactggacg cagaaatgca cgcctgggtg gacagcgcct
     2821 ctgcaggcca gaagctggag atatgtttcc gcagcattcg ggtggtgtgg aagtctggcc
     2881 gcgcgttcgt acaagcacca gacggccacg tgcaggcggc actggacgcc atccttgact
     2941 atg
//
LOCUS       DAOSJM010000317         2930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_29436_length_2930_cov_5.018435, whole genome shotgun sequence.
ACCESSION   DAOSJM010000317 DAOSJM010000000
VERSION     DAOSJM010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29436_length_2930_cov_5.018435"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="PKX82_10525"
     CDS             <1..139
                     /locus_tag="PKX82_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOF52733.1"
                     /translation="KEGPFTDFNGSVEDVNYEKSKVRVSVTIFGRATPVELEFSQIEK
                     T"
     gene            257..688
                     /gene="rplK"
                     /locus_tag="PKX82_10530"
     CDS             257..688
                     /gene="rplK"
                     /locus_tag="PKX82_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOF52734.1"
                     /translation="MAKKIVGFIKLQVPAGKANPSPPIGPALGQRGLNIMEFCKAFNA
                     QTQGVEPGLPLPVVITAFADKSFTFIIKTPPATVLIKKAIKLDKGSARPHMDKVGKIT
                     RAQLEEIAKTKMKDMNAADLDAAVRTIAGSARSMGVNVEGV"
     gene            689..1384
                     /gene="rplA"
                     /locus_tag="PKX82_10535"
     CDS             689..1384
                     /gene="rplA"
                     /locus_tag="PKX82_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOF52735.1"
                     /translation="MSKLTKKQKTQVGKIDSNKLYPLGDALGLVKEFANAKFDESIDV
                     AVQLGIDAKKSDQVVRGAVVLPNGTGKTKRVAVFAQGAKAEEAKAAGADVVGMDDLAA
                     MVKAGDMPFDVVIAAPDAMRIVGTLGQILGPRGLMPNPKVGTVTPDVATAVKNAKAGQ
                     VQFRVDKAGIVHSTIGRRSFESDKLQGNLAALVDALNKAKPASSKGVYLRKVAVSSTM
                     GVGVRVDIQSIAA"
     gene            1646..2173
                     /gene="rplJ"
                     /locus_tag="PKX82_10540"
     CDS             1646..2173
                     /gene="rplJ"
                     /locus_tag="PKX82_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOF52736.1"
                     /translation="MSLNRSEKEAVISDVTGLAAKAQTLVIAEYRGITVADMTKLRVA
                     ARSAGVTLSVLKNTLARRAVAGSSFEIVSDQMTGPLIYGFSEDAVAAAKVVAEFAKTN
                     DKLVIRAGAYGGRALDVNGVKQLASIPSKEVLLAQLLGLMQSPISRTARVLAALAEQR
                     GAGAAEAAPAEAVAA"
     gene            2216..2593
                     /gene="rplL"
                     /locus_tag="PKX82_10545"
     CDS             2216..2593
                     /gene="rplL"
                     /locus_tag="PKX82_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOF52737.1"
                     /translation="MAFDKDAFLTALDSMTVMELNDLVKAIEEKFGVSAAAMAAPAAA
                     GAGAGAAAAEEKTEFNVMLMEAGANKVSVIKAVREITGLGLKEAKDLVDGAPKAVKEG
                     IAKADAEAAKKKLEEAGAKVELK"
BASE COUNT          669 a          753 c          876 g          632 t
ORIGIN      
        1 caaggaaggc ccatttaccg acttcaatgg ttcggtggaa gacgtcaact acgagaagag
       61 caaggtgcgc gtgtctgtga ccatctttgg tcgtgcgaca cccgtcgagc tcgagttttc
      121 tcaaatcgag aagacctgaa ggttttgcgt tgcatttttc aactcggtgc gagcggtttt
      181 tgtagagttg cttaccccgg ggagctgcgg tctgagtggt tcaggtccaa agcgctatta
      241 cccgtaagga gaaatcatgg cgaagaaaat cgttggtttc atcaagctgc aagtgccagc
      301 tggtaaagcc aatccatccc cccctattgg cccggctctg ggtcagcgcg gcctgaacat
      361 catggaattc tgcaaggcat tcaatgccca gacccaaggt gtcgagcccg gactgccatt
      421 gcctgtggtg atcacggcat ttgcggacaa gagctttacc ttcatcatca agaccccgcc
      481 tgcgacggtt ttgatcaaga aggccatcaa gctggacaag ggttccgcac gtcctcacat
      541 ggacaaggtc ggcaagatca cccgcgctca gctggaagaa atcgccaaga ccaagatgaa
      601 agacatgaac gcagccgatc tggatgccgc tgttcgtacc atcgctggtt ctgcccgctc
      661 catgggcgtg aatgtggagg gcgtgtaaat gtccaagctc accaagaaac aaaagaccca
      721 agttggcaag attgacagca acaagctgta ccctttgggc gatgcgctcg gactggtgaa
      781 ggaattcgcc aacgccaagt ttgatgagtc catcgacgtg gccgtgcagc tcggcattga
      841 tgccaagaag tctgaccagg tggtgcgtgg tgctgtcgtg ttgcccaatg gcaccggcaa
      901 gaccaagcgc gtggctgtgt ttgcccaggg tgccaaggct gaagaagcca aggctgctgg
      961 cgctgacgta gtcggcatgg atgacctggc tgccatggtg aaggctggcg acatgccctt
     1021 cgacgtggtg attgctgcac ccgatgccat gcgtatcgtc ggtacgctgg gccagatctt
     1081 gggcccacgt ggcctgatgc ctaatcccaa ggttggcacc gtgactcccg atgtggctac
     1141 tgcggtaaaa aatgccaagg ctggccaggt ccagttccgt gtggacaaag ccggtatcgt
     1201 gcacagcacc attggtcgtc gttcttttga atccgacaag ctgcagggta acctggcggc
     1261 tctggtggat gcactgaaca aggccaaacc tgcttccagc aagggcgtgt atttgaggaa
     1321 agtcgctgtt tcgtcgacca tgggtgtggg tgtccgcgtg gacatccagt ccatcgcggc
     1381 gtaatcgcaa aaattggact attccctcgg gtgtagtccg gtgtggtggg ctgtagaagc
     1441 tgaaaggcaa ctacaggtca tccaagaccg ttggtgtgcg agcaatcgca cttaatcatg
     1501 gggtcagaag gttcgcctga agactctggc caacgcagat ggcgatcccg ctgcagatgg
     1561 aatgtgagtt ctgttaacag ttggtcgctg caatgtggag cgtaaaagcg gcatttgctg
     1621 ccaagcgcat ttaaaggagt agaccttgag tctgaatcgc agtgagaaag aagcggtcat
     1681 cagtgatgtg accggcctcg ccgctaaagc tcaaacgctc gtgatcgcgg aataccgcgg
     1741 catcacggtt gcggacatga ccaaattgcg tgttgccgcc cgtagcgccg gtgtgaccct
     1801 gagtgtgttg aaaaacacct tggctcgccg tgctgtggct ggtagcagct ttgaaatcgt
     1861 gtcggaccag atgaccggtc ccctgatcta cggcttttcc gaagacgcag tagctgccgc
     1921 caaggtggtg gccgagttcg cgaaaaccaa cgacaagttg gtgatccgtg ctggtgcgta
     1981 cggcggtagg gctttggacg tgaacggcgt taaacagctc gcaagcatcc cttccaagga
     2041 agtgttgttg gcgcagttgc tgggcttgat gcaatccccc atttcgcgta cagcccgtgt
     2101 gctggccgca ctggcggagc aacgtggcgc tggcgcagca gaagctgcac ccgcagaggc
     2161 agttgcagcg taattgctgt ctgtccgtag taaccaatat tgttaggaaa tcaaaatggc
     2221 attcgataaa gacgcatttt tgaccgcgct ggacagcatg acggtcatgg aactcaacga
     2281 cctggtgaaa gccatcgaag agaaatttgg cgtgagcgca gctgctatgg ctgctcccgc
     2341 tgctgctggc gctggcgctg gcgctgctgc cgcagaagag aagactgaat tcaacgtgat
     2401 gttgatggaa gctggcgcca acaaggtgtc cgtcatcaag gctgtgcgtg aaatcactgg
     2461 cctgggtctg aaagaagcca aggatctggt ggacggcgct cccaaggctg tcaaggaagg
     2521 cattgccaag gctgacgctg aagctgccaa gaagaagctg gaagaagctg gcgccaaggt
     2581 cgaactcaag taatcgactg ttgcttacag gctggggtgc tccaaaaggg cccccagcct
     2641 ttgctgcttg taagagcgca ctaaaaagca gtggccccac tgttttttag tgtcttctga
     2701 tcccgactgc agaagatgcc ttggtacggg ttacgtgcaa tgcgtaaccg tccgccaatg
     2761 attggtagtg gccaatcacc aagcctgttg agagtgacgc tcaaccgaca gtcgcctagg
     2821 acccccaggt tcctttagtc tttgcccgga gatcgaatgg cttatacata cactgaacgc
     2881 aagcggattc gcaaaaattt cggcaaccgc gacagcgtgc tcgaaatccc
//
LOCUS       DAOSJM010000318         2927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_29488_length_2927_cov_3.914694, whole genome shotgun sequence.
ACCESSION   DAOSJM010000318 DAOSJM010000000
VERSION     DAOSJM010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29488_length_2927_cov_3.914694"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKX82_10550"
     CDS             <1..269
                     /locus_tag="PKX82_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOF52738.1"
                     /translation="AIGALEREVLLQFLIEAVVLSSLGGLIGIVLATGASIGLAAVME
                     VPYLFNPAVNLLSFVFSAGIGVLFGYFPARRAARLDPIEALRHE"
     gene            complement(319..909)
                     /gene="purN"
                     /locus_tag="PKX82_10555"
     CDS             complement(319..909)
                     /gene="purN"
                     /locus_tag="PKX82_10555"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOF52739.1"
                     /translation="MKNIVILISGSGSNMAAIVKTAQQQHWQQKWGARVACVISNKAD
                     AQGLQFAREHGIATEVLDHKAFDSREAFDDALAALIARHDTPERPALVVLAGFMRILT
                     PGFVQRFAGRLVNIHPSLLPAFTGLHTHQRAIDAGCKFAGATVHLVTAELDHGEILDQ
                     AVVPVLPGDTAQALAERVLTQEHVIYPRAVANLLSK"
     gene            923..1585
                     /locus_tag="PKX82_10560"
     CDS             923..1585
                     /locus_tag="PKX82_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HOF52740.1"
                     /translation="MTTPLLLSPIEARVLATLMEKARTVPDSYPLTLNSLMLGCNQKT
                     SRDPLMDLTDADVAAALESLREASLVHETSGGRTTRYTHNFQRGVGVPEQSAVLLGLL
                     MLRGPQTAAELRLNSERWYKFADVSSVEGFLEELQDRSEDKGGPLTVKLPRAPGAREQ
                     RWAHLLCGPVDMTSPVTGQQAAAHGTTADRLERLETEVAELRAILEKLHQELGLSPFG
                     QK"
     gene            1652..2842
                     /locus_tag="PKX82_10565"
     CDS             1652..2842
                     /locus_tag="PKX82_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF52741.1"
                     /translation="MDLAFTPEEQKFREDIRAWVQANLPADIAHKVHNALHLTREDMQ
                     NWAKILGKKGWLGHAWPKEFGGPGWNSVQKHLFEEECALAGAPRVVPFGPVMVAPVIM
                     AFGNAEQQKRFLPGIADGSVWWSQGYSEPGSGSDLASVKTRAEKRTIPGQGTVYVVNG
                     QKTWTTLGQYGEWIFCLVRTSTEGKPQTGISFLLIDMKSPGVTVRPIIMLDGGHEVNE
                     VFFDNVEVPVENLIGEENKGWNYAKHLLAHERTNIADVNRTKRELERLKRIAKQEGVY
                     DDVRFRDEIAKLEVDVVALEMLVLRVLAAEKSGKNPLDIAGLLKIRGSEIQQRYAELM
                     MLAAGPNAVPLVREAMEAGWQGDQAVARCAPLASTYFNLRKTTIYGGSNEVQRNIVSQ
                     VVLG"
BASE COUNT          588 a          926 c          905 g          508 t
ORIGIN      
        1 tggccattgg cgcgctggag cgcgaggtgc tgctgcagtt cctgatcgaa gccgtagtgc
       61 tgtcctcact gggcggactg atcggcatcg tgctggccac cggggcctcg atcgggctgg
      121 cagcggtaat ggaagtgccc tacctgttca accccgcggt gaacctgctg tcgttcgtgt
      181 tttcagcagg cattggcgtg ctgtttggct actttccggc gcgccgcgcg gcgcggctgg
      241 acccaatcga ggcgttgcgc cacgaataat tcaagccaaa ttgacctcca gcccccgtgg
      301 aatatgcgca agtagctgct attttgatag caaatttgcc accgcccggg ggtagatcac
      361 atgctcctgg gtgagcaccc gctccgccag cgcctgcgcg gtatcgcctg gcagcaccgg
      421 caccaccgcc tggtccagaa tttcgccgtg gtccagttcg gctgtcacca gatgcaccgt
      481 ggccccggca aacttgcaac ccgcatcgat ggcccgctgg tgggtgtgga gcccggtgaa
      541 cgccggcaac agcgacggat ggatattgac cagccgcccg gcaaagcgct gcacaaagcc
      601 cggagtgaga atgcgcataa agcccgccag caccaccagg gcgggccgct cgggcgtatc
      661 gtggcgcgca atcaacgccg ccaatgcatc gtcaaacgct tcgcggctgt caaaggcttt
      721 gtggtccagc acttcggtgg caatgccgtg ctcgcgggcg aactgcaggc cctgggcatc
      781 ggccttgttg ctgatgacac aggccacgcg tgcgccccat ttttgctgcc agtgctgctg
      841 ttgtgcagtt ttgacgatgg ccgccatgtt ggagccgctg cctgagatca aaataacgat
      901 gtttttcata ccggcctgca ttatgaccac ccccttgcta ctctccccca tcgaagcccg
      961 cgtactcgcc accctcatgg agaaggcgcg caccgtgccc gacagctacc cgctgaccct
     1021 caatagtctg atgctcggct gcaaccagaa gaccagccgc gacccgttga tggacctgac
     1081 cgatgccgac gtggccgccg cgctggagtc gctgcgcgag gccagcctgg tgcacgagac
     1141 cagcggtggg cgcaccaccc gctacaccca caacttccag cgcggcgtgg gcgtgcccga
     1201 gcaatctgcg gtgctgctgg gcctgctgat gctgcgcggt ccgcagacgg cggcggaatt
     1261 gcgcctcaac agcgagcgct ggtacaaatt tgccgacgtg tcctcggtgg agggctttct
     1321 ggaggagttg caggaccgct cggaagacaa gggtggcccg ctgaccgtca agctgccccg
     1381 tgcgccgggc gcgcgcgagc agcgctgggc gcacttgctg tgcggcccgg tggacatgac
     1441 cagcccagtg acgggccagc aggcggctgc gcacggcacg actgcagacc ggctggagcg
     1501 gctggaaacc gaggtggccg agctgcgcgc catcctggaa aaactgcacc aggaactggg
     1561 tttgtcaccc tttggacaga agtaatcgaa cgaccgtgct aaaacacggg gttagacgga
     1621 cacctatgac cgattgattg gagaccactg tatggatttg gcatttaccc ccgaagagca
     1681 gaaattccgc gaggacattc gcgcctgggt ccaggcgaac ctgcccgctg acattgcgca
     1741 caaagtgcac aacgccctgc acctgacccg tgaggacatg cagaactggg ccaagatcct
     1801 gggcaaaaag ggttggctcg gccacgcctg gcccaaggaa tttggcggcc cgggctggaa
     1861 ttcggtgcaa aaacacctgt ttgaagaaga gtgcgcactc gctggcgccc cccgcgtggt
     1921 gcccttcggc cccgttatgg tggcaccggt gatcatggct ttcggcaatg ccgaacaaca
     1981 aaagcgcttc ctgcccggca ttgccgacgg ttccgtatgg tggagccagg gttacagcga
     2041 gcccggttcc ggctctgacc tggcttccgt caagacccgc gcagaaaaga gaacaatacc
     2101 aggccagggc accgtctatg tcgtgaacgg ccagaaaacc tggactaccc tgggccagta
     2161 cggcgaatgg atcttctgcc tggtgcgcac cagcaccgag ggcaagcccc agaccggcat
     2221 cagcttcctg ctgatcgaca tgaaatcgcc cggcgtcacc gtgcgcccga tcatcatgct
     2281 tgacggcggc cacgaagtga acgaagtgtt cttcgacaac gtcgaagtgc ccgttgaaaa
     2341 cctgattggt gaagagaaca agggctggaa ctacgccaag cacctgctgg cacacgagcg
     2401 caccaacatt gccgacgtga accgcaccaa gcgcgagctg gagcgcctca agcgcattgc
     2461 caagcaggaa ggcgtgtacg acgacgtgcg cttccgtgac gaaatcgcca agctcgaagt
     2521 cgatgtggtg gcgctggaaa tgctggtgct gcgcgtactt gccgccgaaa agtccggcaa
     2581 gaacccgctg gacattgccg gcctgctcaa gatccgcggc agcgagatcc agcaacgcta
     2641 tgccgagctg atgatgctgg ccgccggccc caacgccgtc cccctggtcc gcgaggccat
     2701 ggaagccggc tggcaaggcg accaggctgt tgcgcgctgc gcacccctgg catccaccta
     2761 cttcaacctg cgcaagacca ccatctacgg cggcagcaac gaagtgcaac gcaacatcgt
     2821 gtcccaggtc gttctgggct aacaggagat agatatggat ttcgattttt ccgacgacca
     2881 ggaacaactg cgcgacgccg tacgcaagta cgtggacaag ggctaca
//
LOCUS       DAOSJM010000319         2896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_29899_length_2896_cov_2.788103, whole genome shotgun sequence.
ACCESSION   DAOSJM010000319 DAOSJM010000000
VERSION     DAOSJM010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29899_length_2896_cov_2.788103"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..>1393
                     /locus_tag="PKX82_10570"
     CDS             110..>1393
                     /locus_tag="PKX82_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52742.1"
                     /translation="MLRSTPMIQAAIVLACTHALMPALAQNPSTVVGPYFNRIATFEA
                     HRNVPAGRTVSKKSVAEIVAASADGRWLAYTDAEQQGIGFIDIANPAQPQPAGFVPVD
                     GEPTSVVFAHDKVLAVVSSTRQFDQPEGHLAVLDWASRKVVAKCNLYGQPDSIALDPG
                     ARHAVIAMENERDEKRNKGAMPQLPGGNLSIVPLDAKGMPDCTRLHPVALDGLADIAT
                     GDPEPEFVKVNRQGVAVVSLQENNHLVLVDVAKRTVLGHFSAGSVNLKEVDSKRDGNI
                     QPTDTLNNVMREPDAVAWLDDTRFVTANEGDYKGGSRSFSIFNTDGSVAWDSGNFLDH
                     EAIRLGHYPEARSAAKGNEPEGIEVGLFGKDRLIFVGSERSSLVTVWRDRGVGQPPQY
                     LQALPAGSGPEGLLAIPQRNLLVVASENDGAARSSV"
     assembly_gap    1394..1403
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1404..2358
                     /locus_tag="PKX82_10575"
     CDS             <1404..2358
                     /locus_tag="PKX82_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HOF52743.1"
                     /translation="MIYQRGAAEPAYPTLMSSDSASGTPIPWGAISGFSADRKQPGSL
                     WAVTDSVYATTRILQIDANFSPALVTSAITVTKGGKPVGFDGEGIAQRADGGFWIASE
                     GDPDKKGGALKDLLVRVNAKGEVQEEIALPESLSRHAVRFGLEGVTTTGEGEQEAVWL
                     AVQREWKDDPKGMVKILRYTPATQAWGVLHYPLDTTTASGGWVGLSEITAVGNDTLVV
                     IERDNQFGDQSLKTLKAFSVAGLQPAAPGDANVPVVRKRLVRNLVPDLQKQRGYVLDK
                     VESFAIDAAGDAFAVTDNDGVEGGASGETQFLRLGKLPALR"
     gene            complement(2355..>2896)
                     /gene="queA"
                     /locus_tag="PKX82_10580"
     CDS             complement(2355..>2896)
                     /gene="queA"
                     /locus_tag="PKX82_10580"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOF52744.1"
                     /translation="QRYQTVFARAPGAVAAPTAALHFDEGVLADLAARGIERASVTLH
                     VGAGTFQPVKTENLAEHQMHSEWYEVPQATQDAIADCKARGGRIIAVGTTTVRTLESW
                     AQSGKTSGDTTIFITPGFAFQLVDLLVTNFHLPKSSLMMLVSAFAGYDHIMTLYREAI
                     AREYRFFSYGDAMLLERQG"
BASE COUNT          522 a          891 c          965 g          508 t
ORIGIN      
        1 attgtcctgg atacccgcgc gcaagccgct cgcgccgggt tggggtagcc gtcagtcacc
       61 aaattgtcac agcgcagaca taggatgccg gcttacttta ggacggtcca tgttgcgctc
      121 tacacccatg attcaggcag ccattgtgct ggcttgcacc cacgccctga tgcccgcgtt
      181 ggcacaaaac ccttccacgg tggtcggccc gtatttcaac cgtatcgcca ccttcgaggc
      241 acatcgcaac gtcccggccg ggcgcacggt cagcaaaaag tcggtcgccg aaattgtggc
      301 ggccagtgcc gatggccgct ggctggccta taccgatgcc gagcagcagg gtattggctt
      361 catcgacatt gccaaccccg cgcagcccca acccgccggt tttgtgccgg tggacggcga
      421 gcccacttcg gtggtgtttg cgcatgacaa agtgctggcg gtggtgagtt ccacgcgcca
      481 gtttgaccag cccgaggggc acctcgcggt gctcgattgg gccagtcgca aggtggtggc
      541 gaaatgcaat ctgtacggcc agccggactc catcgccctg gatcccggag cccggcacgc
      601 agtcatcgcg atggaaaacg agcgcgacga aaagcgcaac aaaggggcca tgccccagtt
      661 gcccggcggc aatctgagca ttgttccgct ggatgcgaag ggcatgccgg actgcacgcg
      721 cctgcatcct gtggcattgg atggcctggc cgacattgcg acgggtgatc cggaacccga
      781 gttcgtcaag gtcaatcgcc agggtgtggc tgtggtgtcc ttgcaggaga acaaccacct
      841 ggtgctggtc gatgtcgcca agcgcaccgt gttggggcac ttttccgccg gcagcgtgaa
      901 cctcaaggaa gtggacagca agcgcgatgg caacatccag cccaccgaca ccctgaacaa
      961 tgtcatgcgg gagcccgatg ctgtggcctg gctggacgac acgcgctttg tgacagccaa
     1021 cgagggcgac tacaagggtg ggtcgcgcag tttctccatc ttcaacaccg acggcagcgt
     1081 ggcctgggac agcggcaact tcctggacca tgaggccatc cgtctggggc actacccgga
     1141 agcgcgttcc gccgccaagg gcaatgagcc cgagggcatt gaggtcggcc tgttcggcaa
     1201 ggaccgactg atttttgtcg gctccgaacg ttcctcgctg gtgacggtgt ggcgtgaccg
     1261 gggtgttggg cagccgccgc agtatctaca ggcgctgccg gcaggcagtg ggcccgaagg
     1321 cctgctggcg attccgcagc gcaacttgct ggtggtggcc agcgagaacg atggtgcagc
     1381 ccgctccagc gtgnnnnnnn nnngatgatc taccagcgtg gtgctgccga accggcatac
     1441 cccactctga tgtccagtga cagcgccagc ggtaccccga ttccctgggg cgcgatctct
     1501 ggcttctcgg ccgaccgcaa gcagccggga agcctgtggg ctgtcaccga cagtgtctat
     1561 gccacgacgc gtattctgca gattgacgcc aacttcagcc cggcgctggt gacgtctgcc
     1621 atcaccgtca ccaaaggcgg caaaccggtc ggatttgacg gtgaaggcat tgcccagcgc
     1681 gccgatggcg ggttctggat tgcatccgag ggagatccgg acaagaaggg aggcgccctg
     1741 aaggacctgc tggtgcgtgt gaacgccaag ggcgaagtgc aggaagagat cgccttgccc
     1801 gaaagcctga gccgccatgc cgtgcgcttc ggactcgaag gcgtgacgac caccggcgag
     1861 ggggagcagg aagccgtgtg gctggccgtg cagcgtgagt ggaaagacga tcccaagggg
     1921 atggtgaaga ttctgcgcta cacccccgcc acccaggcct ggggcgtact gcactacccg
     1981 ctggacacca cgaccgcatc gggtggctgg gtgggtttgt ccgaaattac ggccgtgggc
     2041 aacgacaccc tggtggtgat tgagcgcgac aaccagtttg gcgaccagtc gctcaagaca
     2101 ctcaaggcct tctccgtggc cgggctgcag cctgctgccc cgggtgatgc caacgtgcct
     2161 gtcgtgcgca agcgcctggt gcgtaacttg gtgccggatt tgcagaagca gcgcggctat
     2221 gtgctcgaca aggtggagag ctttgccatc gatgcagcgg gcgacgcctt tgccgtcacg
     2281 gacaacgacg gcgtggaagg cggggccagc ggtgaaacgc agttcctgcg tctgggtaaa
     2341 ctgccggccc tgcgctaacc ctggcgctcc agcagcatgg catcgccata gctgaagaag
     2401 cggtactcgc gcgcaatcgc ctcgcggtac agcgtcatga tgtggtcgta gcctgcaaag
     2461 gcgctgacca gcatcatcag gctcgatttg ggcaggtgga agttggtcac cagcaggtcc
     2521 accagctgga acgcaaaccc cggcgtgatg aagatggtgg tgtcgccgct ggtcttgccg
     2581 ctctgggccc agctttccag tgtgcgaacc gtcgtggtgc ccaccgcgat gatgcggccg
     2641 ccacgcgcct tgcagtcggc aatggcgtcc tgtgtggcct gcggaacctc gtaccactcg
     2701 ctgtgcatct ggtgctcggc caggttctcg gtcttgacgg gctggaaggt gccggcgccc
     2761 acgtgcagcg tgacactcgc gcgctcgata ccgcgcgcag ccaggtcggc cagcacgccc
     2821 tcgtcaaagt gcagcgcggc ggtgggcgcg gccacggcgc cgggggcgcg cgcaaacacg
     2881 gtctggtagc gctggc
//
LOCUS       DAOSJM010000320         2885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_30047_length_2885_cov_3.657244, whole genome shotgun sequence.
ACCESSION   DAOSJM010000320 DAOSJM010000000
VERSION     DAOSJM010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30047_length_2885_cov_3.657244"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..712
                     /gene="phnH"
                     /locus_tag="PKX82_10585"
     CDS             74..712
                     /gene="phnH"
                     /locus_tag="PKX82_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HOF52745.1"
                     /translation="MKAHDLSALGAGFSSEAHGSQSVFREALQALSHPGRCVDALHDA
                     QVPSAGHPASAALLLAMLDADCTLWLSPMLARSDAALWLHFHTGCQLVDDLAQAQFVW
                     VAAGDAMPELKVLPQGTEAYPDQSATCVVDVLTLQAHLQGQAGWCLRGPGIQGQAQLR
                     VEGVPADFLAQWSANHARFPRGVDVFLAAQQQLVGLPRTTAIEPTGGQSACM"
     gene            703..1794
                     /locus_tag="PKX82_10590"
     CDS             703..1794
                     /locus_tag="PKX82_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-phosphorus lyase complex subunit PhnI"
                     /protein_id="HOF52746.1"
                     /translation="MYVAVKGGETAILNSYQLLAEQRRGDHALPELSVPQIQQQLKLA
                     VDRVMNEGSVYDSELAALAIKQASGDLVEAIFLLRAYRATLPRLGTSKPLHTERMQLD
                     RRVSATFKELPGGQVLGPTYDYTQRLLDFTLLAQGESAVPAVKPNAPVVMSESSFTPR
                     VVDLLNDEGLIETRPIPPGDPAPVDLTREPLRFPAVRSTRLQNLARGDEGFLLAMAYS
                     TQRGYAHSHPFVGEVRLGSVELEIEPEELGFAISIGDLEITECEMVNQFAGSKTEPPQ
                     FTRGYGLAFGHCERKAMAMSLVDRALRADELGESIESPAQMQEFVLSHSDSLEASGFV
                     QHLKLPHYVDFQSELELIRKMRATTEVTP"
     gene            1803..2696
                     /locus_tag="PKX82_10595"
     CDS             1803..2696
                     /locus_tag="PKX82_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HOF52747.1"
                     /translation="MKTENLTPATHGEDDVQSHFNFAYLDERTKRMIRRAILKAVAIP
                     GYQVPFGSREMPLPYGWGTGGIQVTASVIGPDDVLKIIDQGSDDTVNAVNIRRFFQRT
                     TGVATTTRTVDATLIQTRHRIPEIPLAEGQVIVYQVPVPEPMQRLEPRETETRTLHGL
                     AEYGLMHVKLYEDIARYGHIATTYDYPVLVNDRYIMAPSPIPKFDNPKMHMNPALQLF
                     GAGREKRIYAVPPYTSVESLGYEDHPFEVQRWDAACALCGATDSFLDEVITDDAGTRM
                     HVCSDSDYCQSRQAVEPGAQA"
     gene            2693..>2885
                     /locus_tag="PKX82_10600"
     CDS             2693..>2885
                     /locus_tag="PKX82_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF52748.1"
                     /translation="MTLQIRQATQRDIASVLDLYAQPGLDDGQVLSEEAAQEVFAQFS
                     RYPNYRLFVALDGDAVVGTY"
BASE COUNT          541 a          900 c          906 g          538 t
ORIGIN      
        1 cccaaagtac caaggtggag ttcttcaccg tagcccgtga atccggaacc cctggcgaag
       61 aagaggaaaa cgaatgaaag cccatgacct gtccgcactg ggtgcgggtt tttccagcga
      121 ggcgcatggc agccagtcgg tctttcgtga ggcattgcag gccctgtccc atccggggcg
      181 ctgtgtggat gcgctgcatg acgcgcaggt gccgtcggct ggccatcccg cctcggcagc
      241 gttgctgctg gccatgctgg atgccgattg cacgctgtgg ctgtcgccca tgctcgcccg
      301 ctctgacgcg gcgctgtggc tgcactttca taccggttgt cagttggtgg atgacttggc
      361 gcaggcccag tttgtgtggg tggcggctgg ggatgccatg ccggagttga aggtcctgcc
      421 gcaaggcacc gaggcctatc ccgaccagtc tgccacctgc gtggtcgatg tgctgactct
      481 gcaagctcac ttgcaggggc aagccggttg gtgcttgcgc gggccgggca tccagggcca
      541 ggcccaactg cgcgtagaag gggtgcccgc agattttctg gcgcagtggt ctgccaacca
      601 cgcccgtttt ccacggggtg tcgatgtgtt cctggcagcg caacagcagc tggtgggcct
      661 gccgcgcacc accgccattg aaccaaccgg agggcagtca gcatgtatgt agccgtcaaa
      721 ggcggtgaaa ccgccatcct caacagctac cagctgctgg ccgaacagcg caggggtgac
      781 catgccttgc cggaactcag cgtgccgcag atccagcagc agctcaagct ggcggtggac
      841 cgggtgatga acgaaggttc ggtgtacgac tccgaactgg ccgcgctggc gatcaagcag
      901 gcgtcgggcg acctggtgga agcgatcttc ctgctgcgcg cctaccgcgc cacattgccg
      961 cgcctgggga cgtccaaacc tctgcacacc gagcgcatgc agctggatcg ccgtgtgtcg
     1021 gccacgttca aggagctgcc gggcggacag gtgctgggcc cgacgtacga ctacacccag
     1081 cggctgctgg acttcacctt gctggcgcaa ggcgaatcgg cagttcccgc cgtcaagcca
     1141 aacgcaccgg tggtcatgag cgagagttcg tttaccccgc gtgtggtgga tctactcaac
     1201 gacgagggat tgattgaaac ccgcccaatt cccccgggcg accccgctcc ggtggacctg
     1261 acccgcgaac ccctgcggtt tccggcggtg cgttccaccc gcctgcaaaa cctggcgcgg
     1321 ggcgatgaag gatttctgct ggccatggcc tattcgaccc agcgcggcta tgcccacagc
     1381 cacccgtttg tcggcgaggt gcggctgggc agcgtggaac tggaaatcga gccggaagag
     1441 ctgggctttg ccatctcgat tggcgatctg gaaattaccg aatgtgaaat ggtcaaccag
     1501 tttgctggca gcaagaccga gccgccgcag ttcacccgtg gttatggcct ggcctttggc
     1561 cactgtgaac gcaaagccat ggccatgagt ctggtggacc gggctctgcg cgccgatgaa
     1621 ctgggcgagt cgattgaatc gccagcgcag atgcaggagt ttgtgctgtc ccacagcgac
     1681 agcctggaag cctccggatt tgtgcagcac ctgaagctgc cgcactacgt ggactttcag
     1741 tccgaactgg agctgatccg caagatgcgc gctaccaccg aggtgacccc atgaacgcac
     1801 ccatgaaaac cgaaaatctg actccggcca cccatggcga agacgatgtg cagtcgcact
     1861 tcaactttgc ctacctggac gagcgcacca agcgcatgat ccgccgcgcc atcctcaagg
     1921 cggtggctat tcctggttac caggtgccct ttggctcgcg ggaaatgccg ctgccgtatg
     1981 gctggggcac tgggggcatc caggtcacgg cctccgtgat cgggccggat gatgtgctga
     2041 aaatcatcga ccagggctcg gacgatacgg tcaatgccgt caatatccgt cggttcttcc
     2101 agcgcaccac gggtgtggcc accaccaccc gcacggtgga cgccacgctg atccagacgc
     2161 gccaccgcat tccggaaatc ccgctcgccg aaggccaggt catcgtctac caggtgccgg
     2221 tgcccgaacc catgcagcgc ctggaaccgc gcgagaccga gacgcgcacc ctgcatggcc
     2281 tggcggaata cggcctgatg catgtgaagc tgtacgagga cattgcccgc tacggccaca
     2341 ttgccaccac ctacgactat ccggtgctgg tgaatgaccg ctacatcatg gcgccgtcac
     2401 cgatcccgaa gttcgacaac cccaagatgc acatgaatcc cgcgctgcag ctgttcggtg
     2461 cggggcgcga gaagcgcatt tacgccgtgc caccttacac atcggtcgag agtttgggct
     2521 atgaagacca tccgtttgaa gtgcagcgct gggatgcggc gtgtgccctg tgtggtgcca
     2581 ctgacagctt ccttgacgaa gtgatcaccg atgatgctgg cacccgcatg catgtgtgct
     2641 cggactcgga ctattgccag tcccgccaag ccgttgaacc aggagcgcaa gcatgacgct
     2701 acagatacgc caggccacac agagagacat tgcgtcggta ttggacctct acgcacagcc
     2761 cggtctggac gacggccagg tgctttcgga ggaggctgcg caagaagtgt ttgcccagtt
     2821 ttcccgctac cccaattacc ggttgtttgt cgcgctggat ggggacgctg tggtcggcac
     2881 ctacg
//
LOCUS       DAOSJM010000321         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_30082_length_2883_cov_3.164427, whole genome shotgun sequence.
ACCESSION   DAOSJM010000321 DAOSJM010000000
VERSION     DAOSJM010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30082_length_2883_cov_3.164427"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PKX82_10605"
     CDS             complement(<1..207)
                     /locus_tag="PKX82_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOF52749.1"
                     /translation="MSTPADARVWRVGALCRAVADALDARFNPVTVSGEISGFSRATS
                     GHCYFVLKDETGQLRCAMFRRAASL"
     gene            516..1142
                     /locus_tag="PKX82_10610"
     CDS             516..1142
                     /locus_tag="PKX82_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOF52750.1"
                     /translation="MFSIIQAAGWPIWPLIACSVLALAIIIERFISLKTSKVAPNRLL
                     EEVLGVIRTGVPGPDVITQLERNSALGEVLASGLRAVNADPRCSEDDLRATMEGAGRV
                     VTQRLEHYLSALATIASAAPLMGLFGTVVGMIEIFGSQAPGGSTGGNPAQLAHGISIA
                     LYNTAFGLIVAIPSLIFWRYFRGRVDAYLLTLELAAEHLVRHLKSLRK"
     gene            1149..1577
                     /locus_tag="PKX82_10615"
     CDS             1149..1577
                     /locus_tag="PKX82_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOF52751.1"
                     /translation="MNFRPRTKDEPEINLIPFIDVLLVVLIFLMLSTTYSKFTEMQLK
                     LPVADTEAQRDYPKEVVVAVSAEGNYSINRVPVAGRSLDTLSAALREGAKAGKDSVVI
                     ISADASARHQAVVTVMEAARRAGLNHITFATQSSAQAGGN"
     gene            1582..2658
                     /gene="lpxK"
                     /locus_tag="PKX82_10620"
     CDS             1582..2658
                     /gene="lpxK"
                     /locus_tag="PKX82_10620"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOF52752.1"
                     /translation="MGSPATKPSLESTLQRIWTRRGAGAWLLLPLSLLYVALSTFRRL
                     LFRTGILTIHRIRVPVIVVGNVIAGGAGKTPTTIAIVQHLQSQGRRVGVISRGYGRKS
                     QETLEVIADTSTELAGDEPLLIRRKTSAPVFVGRSRYQAAQALLARYPDTELIICDDG
                     LQHFSLYRDLEICVFDDRGCGNGWLLPSGPLRETWPRRPLKQAGQRNDRLLVLHTGGQ
                     PRFSGFRAQRSLAAHAVRSNGETVALDTLQKPGALPLMAVAAIAQPAAFFRMLELCGL
                     VIAKTLPLPDHYDFDSFIRNEYGGYQLICTEKDTAKLWQKAPDALAIPLTQTMEPAFW
                     RELDRQIAGLSPTKLSSPHGHKTT"
     gene            2639..2821
                     /locus_tag="PKX82_10625"
     CDS             2639..2821
                     /locus_tag="PKX82_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOF52753.1"
                     /translation="MDTRLLELLVCPVTKGPLEFDREKQELISRSARLAYPVRDGIPV
                     LLENEARTLSDEELGL"
BASE COUNT          590 a          940 c          802 g          551 t
ORIGIN      
        1 aaggctggca gccctgcgga acatggcgca gcgcaactgc cctgtttcat ccttgagaac
       61 gaagtagcaa tgcccgctgg tcgcccgcga aaagccactg atctcgccgg agacggtaac
      121 cggattgaac cgcgcgtcca gcgcgtcggc cactgcgcgg cacagggcac cgacccgcca
      181 aacgcgtgcg tcagcgggcg tggacatcac attacccatg cagaccgccc cgatgcccag
      241 gtaacagcag gcaaaaggtc atccccatgc gagccatccc ctgcgcacag ctaccgcgat
      301 ctcccgcaaa gcacggcgac acgtaaaaga tggttgattt cattggtttt tttgctgcct
      361 catttgtcat caatctgtca aaaccgcggc ccggcgcggt cccgcaccgt ctccaccgca
      421 ggatttccac aaagttatcc acaaaaactg tgcgtccgtg cggacccggt agtgggacat
      481 aattgcacag catctgaatt gcacggagtc actacttgtt ttccatcata caagccgctg
      541 gttggcccat ttggccactg atcgcatgct cagtattggc actggccatc atcattgaac
      601 gtttcatcag ccttaaaacc agcaaagtcg cccccaaccg tctgcttgaa gaggtgctgg
      661 gggtcatccg caccggcgtg ccgggccccg acgtcatcac ccagctggag cgcaattccg
      721 ctttgggtga agtgcttgcc agtggcctgc gcgcagtcaa cgcggacccc cgttgcagcg
      781 aagacgacct gcgcgccacg atggaaggcg ctggccgtgt cgtcacccag cgcctggaac
      841 actacctgag cgcattggcc accattgcct ctgcggcacc actgatgggg ctgtttggca
      901 ccgtggtggg aatgatcgaa attttcggct cccaggcccc cggcggctcc actggcggaa
      961 acccggcaca actggcacat ggcatctcca ttgccctcta caacacagca ttcggcctga
     1021 tcgtggccat tccctcactg atattctggc gctatttccg tggccgcgtg gatgcctatc
     1081 tgctcacgct ggaactggcg gccgagcatc tggtgcggca cctcaaatct ctgcgcaagt
     1141 aattcgcgat gaactttcgc ccccgtacca aggatgaacc cgagatcaac ctgatcccgt
     1201 tcatcgacgt gctcctggtg gtgctgatct ttctgatgct ctccaccacc tacagcaagt
     1261 tcaccgagat gcaactcaag ttgccggttg cggacacaga agcccagcgc gactacccca
     1321 aggaagtcgt agtggctgtc agcgcagaag gcaactacag catcaaccgg gtgcccgttg
     1381 caggccgcag cctggacacc ctgtctgcag cgctgcgcga aggtgccaag gcaggcaagg
     1441 acagcgtggt catcatcagt gccgatgcca gcgcccggca ccaggcagtg gtcaccgtca
     1501 tggaagcagc gcgccgggcc ggtctgaacc acatcacgtt tgcaacccag tcctcggcac
     1561 aggcaggcgg caactaagcc catgggcagc ccggcgacga agccctcgct ggagagcaca
     1621 ctgcagcgta tctggacccg gcgcggggcg ggtgcatggc tgttactgcc cctttctctg
     1681 ctctacgttg cgttgtcaac atttcgacga ctgttgtttc gcaccggcat tctgaccatc
     1741 caccgcatcc gggtccccgt cattgtggtg ggcaatgtca ttgcgggtgg tgcgggaaag
     1801 acacccacca ccattgcaat cgtgcagcac ctgcaaagcc agggccgccg ggtaggcgtt
     1861 atatctcgcg gctatggacg caagagccag gaaaccctgg aagtcatcgc cgatacctcc
     1921 acggaacttg ccggagacga gcccctgctg atccgccgca agacctcggc gccagtgttt
     1981 gtcgggcgct caaggtacca ggctgcacag gccctgctcg ccagataccc cgacaccgag
     2041 ctgatcatct gcgacgatgg actgcaacat ttcagcctgt atcgggacct ggagatatgc
     2101 gtctttgacg accgtgggtg tggcaacggc tggctgcttc cctcgggacc gttgcgggaa
     2161 acctggccac gccgccctct gaagcaagcc ggacaacgca acgaccgcct gctggttcta
     2221 cacacggggg gccagccccg tttttcaggc ttcagggccc agcgcagttt ggctgcgcat
     2281 gccgtgcgct ccaacggcga aaccgtggca ctcgacacgt tgcaaaagcc tggcgcactt
     2341 ccgttgatgg cggtggctgc cattgcccaa ccagcggcat ttttccgcat gctagagctt
     2401 tgtggactgg tgatcgcaaa aacattgccg ctgccagatc actatgattt tgatagcttt
     2461 atccgcaatg agtacggggg ctaccagctg atttgtaccg aaaaagatac cgccaagctc
     2521 tggcagaagg ctcccgatgc cctcgccatc ccgctgaccc agaccatgga acccgcgttc
     2581 tggagagaac tggaccggca gatcgcaggt ctgtcaccta caaagctatc atccccccat
     2641 ggacacaaga ctacttgaac tgctcgtttg ccctgtcacc aagggccccc tggaattcga
     2701 ccgcgagaag caggaactga tttcccgcag cgcgcgcctg gcctacccgg tgcgcgacgg
     2761 catcccggtg ctactggaaa acgaagcccg tacgctgtca gacgaagaac tcggactctg
     2821 agcaccttcc gcatgggata cacggtactg attccggcgc gccttgcttc ctcacgcctg
     2881 ccc
//
LOCUS       DAOSJM010000322         2873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_30207_length_2873_cov_4.964514, whole genome shotgun sequence.
ACCESSION   DAOSJM010000322 DAOSJM010000000
VERSION     DAOSJM010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30207_length_2873_cov_4.964514"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..836
                     /locus_tag="PKX82_10630"
     CDS             93..836
                     /locus_tag="PKX82_10630"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOF52754.1"
                     /translation="MTNQDAPDLQLAAATTVTETPTPASTKPSETQALQNYAIPGSVR
                     LKYEVRGEKSGIPVFANGELLWVQDGKTYEARMEISILMFGRVQTSKGQLGAHGLEPL
                     RFGDKVRSEVAAHFDRSKGKVTFSANTPDVAMLPGAQDQLSIFMHVASMIGGDPRRFP
                     PGTELSFQAIGPRSAETWAFTVGNLETLTLPGGTIKALKLSRPPANEYSPRADVWLAP
                     EMAYMPVRIRLKEANGDIADQQWSATQKP"
     gene            926..1237
                     /locus_tag="PKX82_10635"
     CDS             926..1237
                     /locus_tag="PKX82_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HOF52755.1"
                     /translation="MHTLYDSDTYSVTHMLASAEAPEQHPSGASKSEQWPTSARTLMR
                     HGFEIVDKRAGKEVYLDGSWAELFQQHVMAWQVNTPTQEEVEDTLERYAELAQNPVVV
                     H"
     gene            complement(1270..2079)
                     /locus_tag="PKX82_10640"
     CDS             complement(1270..2079)
                     /locus_tag="PKX82_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOF52756.1"
                     /translation="MKIQLLAAAAFAAVLQTGCAIPPTDPAEVKALRPGILAGYLPAG
                     SWVNSLELLPPPPAAGSAAQAADSAAFQASRKFKDSARWQLAAKDAELMFPKAADTFS
                     CALGVPVSAEATPHLNMLLRRTLADAGLATYRAKDTYKRQRPFMAANDAICTPVEETS
                     LRKDGSYPSGHAALGWAWGLVLTEVAPDRANALTQRAYEFGQSRVVCGVHWQSDVDAG
                     RLVASAVVAQLHGNADFNAQLAQARREYAATRAKAAPQPESCALEAKAFAK"
     gene            2128..>2873
                     /gene="purU"
                     /locus_tag="PKX82_10645"
     CDS             2128..>2873
                     /gene="purU"
                     /locus_tag="PKX82_10645"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOF52757.1"
                     /translation="MSQTYILTLSCPDRLGLVHAVSGFLLERGGNIEEAAQYNDPDTG
                     LFFMRVQFKCSQLTHGDLQTQLRMFATPYQMQWSLHATAQPMRTVLLVSKEGHCLNDL
                     LFRWKSGLLPIDIRAIISNHRDFYQLAASYNVPFHHIAVTAATKAQVEAKQLEIIESE
                     GAELVVLARYMQILSDDLCRKLAGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATA
                     HYVTADLDEGPIIEQDVARVDHSKTVDDLTT"
BASE COUNT          612 a          922 c          830 g          509 t
ORIGIN      
        1 caacggagat caagagaatt cgccctctac ccctcgtaga acctgcgcag aaagctcctg
       61 atttgatagc aaatacagag gcatcacctg cgatgacgaa ccaggacgcg ccagacctcc
      121 agctggcggc ggccaccacc gtcactgaaa cgcccacgcc cgccagcacc aaaccgtcgg
      181 aaacacaggc gctgcagaat tacgcgatac ctggctcggt gcggctgaaa tacgaagtca
      241 ggggtgaaaa aagcggcatt cccgtatttg ccaatggaga gcttctgtgg gttcaggacg
      301 gcaagaccta cgaagcccgc atggaaatca gcatcctgat gtttggccgg gtgcaaacca
      361 gcaagggcca gttgggcgcc catgggctgg agcccctgcg ttttggcgac aaagtgcgca
      421 gcgaagtggc agcgcacttt gacagatcca aaggcaaagt caccttcagc gccaacaccc
      481 ccgatgtcgc catgctgccc ggcgctcagg accagctgag catcttcatg catgtggcgt
      541 ccatgatcgg cggagacccg cgccgctttc caccaggcac cgagctgtcc ttccaggcca
      601 ttggtccgcg gtcggccgag acctgggctt ttaccgtcgg aaacctggaa acactgaccc
      661 tgccaggcgg caccatcaag gcacttaaat tatcgcgccc gcctgccaat gagtattcac
      721 cgcgggccga tgtctggctg gcgcctgaaa tggcctatat gccggtacgc atccggctca
      781 aggaagccaa cggcgatatt gccgaccagc aatggagtgc cacacaaaaa ccctgagtac
      841 gggtctgcgg gccggaaata gctgttactc ttgaaaccgg atcaagtgct cttacatgca
      901 ttgcaacaca ggacaccctc acgccatgca cacactttac gactctgata cctattccgt
      961 gacccacatg ctggccagcg cggaagcgcc ggagcagcac ccgtcgggtg ccagcaagag
     1021 tgagcagtgg cccaccagcg cccgcacact gatgcgccat ggcttcgaaa tcgtggacaa
     1081 acgcgccggc aaggaggtct atctggacgg ctcctgggct gagctattcc agcagcacgt
     1141 catggcctgg caggtcaaca cgcccaccca ggaagaagtg gaagacacac tggagcggta
     1201 cgccgaactg gcgcaaaacc cggtcgtggt tcactgagct ccccgccccg cctcggggca
     1261 gggaatcgtc tatttggcaa aagccttggc ctccagggcg caggattccg gttgcggggc
     1321 tgccttagcg cgtgtagccg catattcccg gcgggcttgc gccaactggg cattgaaatc
     1381 tgcattgcca tggagttgcg ccaccacggc agaggccacc aggcggccag cgtccacatc
     1441 actttgccaa tgcacgccac acaccacacg gctttgacca aactcatatg ccctctgagt
     1501 cagcgcattg gcccggtctg gcgccacctc ggtcagtacc agcccccaag cccagcccag
     1561 cgcggcatgc ccggacggat aagagccgtc cttgcgcaat gaagtttctt ctaccggtgt
     1621 gcagatggca tcgttcgcgg ccatgaaagg acgctggcgc ttgtaagtgt ctttggcgcg
     1681 gtaggttgcc agtccggcat cggccagggt gcggcgcaac agcatattca ggtgcggcgt
     1741 ggcctctgcg ctcaccggga cgcccagggc gcaactgaat gtatccgccg ccttgggaaa
     1801 catcaattca gcatccttgg cagccagctg ccaacgtgca ctgtccttga acttgcgact
     1861 ggcctggaaa gccgcactgt cggccgcctg ggccgcagac cctgcagcgg ggggcggcgg
     1921 cagcagttcc agactgttga cccaagaacc ggcaggcagg tagccggcca ggatgccggg
     1981 gcgcagcgcc ttcacttcgg cagggtccgt tggcggaatg gcacacccag tctgcagaac
     2041 ggcggcgaat gctgctgcgg ccaaaagctg gatcttcatg gcaggtgtct cctgtgggtt
     2101 gggtgttgct gcgacaatcc aaacattatg agtcagacct acatccttac cctgtcctgc
     2161 cccgatcgcc tgggcctggt ccacgccgtc tcgggtttcc tgctggagcg tggcggcaat
     2221 atcgaagagg ccgcacagta caacgacccc gacaccggcc tgtttttcat gcgggtgcag
     2281 ttcaaatgct cccagttgac gcatggtgat ctgcaaaccc agttgcgcat gtttgccacg
     2341 ccctaccaga tgcagtggag tctgcatgcc acggcgcaac ccatgcgcac cgtcctgctg
     2401 gtgagcaagg agggacactg cctcaacgac ctgctgtttc gctggaagag cggcctgctg
     2461 cccatcgata tccgcgccat catttccaac caccgcgact tttaccagct ggctgccagc
     2521 tacaacgtgc ccttccacca cattgcggtc acggcggcca ccaaggcgca ggttgaggcc
     2581 aagcagctgg agatcattga atccgaaggt gccgaattgg tggtgctggc gcgctacatg
     2641 cagatcctga gcgacgacct gtgccgcaaa cttgccggcc gcgccatcaa catccaccac
     2701 tcatttctgc cgagcttcaa gggtgccaag ccttattacc aggcgcacga ccgcggtgtg
     2761 aaactgattg gtgctaccgc ccactacgtg acggcagacc tcgacgaagg cccgatcatc
     2821 gagcaggacg tagcccgggt cgaccacagc aaaaccgtgg acgacctcac cac
//
LOCUS       DAOSJM010000323         2871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_30231_length_2871_cov_5.040128, whole genome shotgun sequence.
ACCESSION   DAOSJM010000323 DAOSJM010000000
VERSION     DAOSJM010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30231_length_2871_cov_5.040128"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..829
                     /locus_tag="PKX82_10650"
     CDS             11..829
                     /locus_tag="PKX82_10650"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOF52758.1"
                     /translation="MRSQYEDFMRHVYTHGVHKGDRTGTGTRSVFGHQMRFDLNEGFP
                     LVTTKKVFLKAIIVELLWFLRGDSNVKWLQERGCTIWDEWARADGDLGPVYGVQWRSW
                     PTPGGDHIDQIADVIHTLKTNPDSRRIIVSAWNVAELDKMALMPCHAFFQFYVAPSET
                     PGGKGRLSCQLYQRSADIFLGVPFNIASYALLTHMVAQQCDLDVGDFIWTGGDCHIYS
                     NHHEQVELQLSRAPKAYPTLHIKRRPDSIFDYQYEDFEVQGYESHPAIKAPVAV"
     gene            826..1716
                     /locus_tag="PKX82_10655"
     CDS             826..1716
                     /locus_tag="PKX82_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF52759.1"
                     /translation="MISRRQLWALLALTLMWGVNWPMMKLSLQGMTPLYFRASTMSLG
                     AAWLFAYVALRGERMWPVGREWQTIAMLGLTNVLGWHTLSIFGVQELASGRAAILGFT
                     MPIFTVLIGAALFGERITARVGLAVVCVAVAVGLLLWHELQRLSGRPAGVVWMLGAAI
                     CWALGTLQIRRAHLTLSPMVLTVWMLLLGSAVLWGLALSFEQPPQTAGFSTRLWISLA
                     YGVLINYGVAQLIWFGMARSLPPATSAMSVMAIPLVGTLSATLIVGEMPHWQDWLAMV
                     FVMVAIASVLLPARPAVPAQ"
     gene            1721..2230
                     /locus_tag="PKX82_10660"
     CDS             1721..2230
                     /locus_tag="PKX82_10660"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOF52760.1"
                     /translation="MTSSSSPRLHLIYARAANGVIGNHNTLPWHLPEDLAHFKRTTLG
                     CPVIMGRKTWDSLPPKFRPLPGRRNIVITRQEKWNENGAYPAQNMQEALQLCEQSADV
                     WVIGGAQLYALAEPLASTAVVTEIDRDFEGNAFAPTLGPAWKETARERHIAASGLPFS
                     FVTYTREAP"
     gene            2227..>2871
                     /locus_tag="PKX82_10665"
     CDS             2227..>2871
                     /locus_tag="PKX82_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOF52761.1"
                     /translation="MKLATWNVNSLGVRLPQVLDWLAAHPVDVLALQELKLTDDKFPV
                     DAFSAAGYSSAWFGQKTYNGVALLSRTPATDVVKNIPGFDDDMSRVITGTIDGVRVIG
                     AYFPNGQEPGSDKFEYKMEWLGALRRWVKEELAQHPRLVLMGDYNITFDDADVWDPEG
                     LRDTIHCTEEERYHLRALIALGLTDSHRLFAHPPKSFSWWDYRDFGFRRNRGLRI"
BASE COUNT          529 a          927 c          865 g          550 t
ORIGIN      
        1 gccccacccc atccgctccc agtacgaaga cttcatgcgc catgtctaca cccacggtgt
       61 gcacaaaggc gaccgcaccg gcaccggcac ccgcagtgtg tttggccacc agatgcgctt
      121 cgacctcaac gaaggcttcc ccctggtcac caccaaaaag gtattcctga aggccatcat
      181 cgtggagctg ctgtggttcc tgcgcggcga cagcaatgtg aaatggctgc aggagcgcgg
      241 ctgcaccatc tgggacgaat gggcccgtgc cgatggcgac ctcggaccgg tctacggcgt
      301 gcaatggcgc agctggccta ccccgggcgg tgaccacatc gaccagatcg ctgacgtcat
      361 ccataccctc aagaccaacc ccgacagccg ccgcatcatc gtaagcgcct ggaacgtggc
      421 cgagctcgac aagatggcgc tcatgccctg ccatgccttt ttccagttct acgtggcgcc
      481 cagtgaaaca ccgggcggca aaggccgtct gagctgtcag ctgtaccagc gcagcgctga
      541 catctttctg ggcgtgccat tcaatattgc cagctacgcc ctgctcaccc acatggtggc
      601 gcagcagtgc gatctggacg tgggcgattt catctggacc ggtggcgact gccacatcta
      661 cagcaaccac cacgaacagg tggagctgca gctcagccgt gcaccgaagg cctaccccac
      721 actgcatatc aagcgcagac ccgactcgat cttcgactac cagtacgagg actttgaggt
      781 gcagggatac gagagccacc ctgccatcaa agcccccgtc gcagtgtgat cagccgccga
      841 cagctgtggg ccctattggc cctcaccctc atgtggggtg tgaactggcc catgatgaag
      901 ctctccctgc agggcatgac cccgctgtac ttccgtgcca gcaccatgtc gctgggcgcg
      961 gcctggctgt ttgcctacgt ggcgctgcgc ggcgagcgca tgtggccggt cgggcgcgaa
     1021 tggcagacga ttgcgatgct tggactgacc aatgtgctgg gctggcacac gctgtcgatt
     1081 tttggcgtgc aggagctggc ctcgggtcgg gcggccattt tgggattcac catgcccatc
     1141 ttcacggtgc tgatcggcgc cgcgctgttt ggcgagcgca tcactgcgcg cgtgggcctg
     1201 gcagtcgtct gtgtcgccgt ggcggtaggg ctgctgctgt ggcacgaatt gcagcgcctc
     1261 agcggccgac ccgccggagt ggtctggatg ctgggagccg ccatttgctg ggccttgggc
     1321 accttgcaga ttcgcagggc ccatctgaca ctctctccca tggtgctcac cgtatggatg
     1381 ctgctgctgg gcagtgcggt gttgtggggg ttggcgctgt ccttcgagca gccaccacaa
     1441 accgccggat tctctacacg cctgtggatc agcttggcat atggcgtact catcaactac
     1501 ggtgtggccc agctgatctg gtttggcatg gcgcgcagcc tgccaccggc caccagcgcc
     1561 atgagcgtga tggccattcc gctggtgggc acgctcagcg ccaccctgat agtgggcgag
     1621 atgccacact ggcaggactg gctggccatg gtgttcgtca tggtggccat tgccagcgtg
     1681 ctgctgccag cgcggcctgc cgtaccggca caataagacc atgacaagtt cgtccagccc
     1741 ccgcctgcac ctgatctatg cccgcgccgc caatggcgtg attggcaacc acaacaccct
     1801 gccctggcat ttgcccgagg acctggcgca cttcaagcgc accacactgg gctgtccggt
     1861 catcatgggg cgcaagacct gggactcctt gccaccgaaa tttcgcccgc tgcccgggcg
     1921 caggaatatc gtcatcaccc gccaggaaaa atggaatgaa aatggtgcct accccgcaca
     1981 gaatatgcag gaggcgctac aactttgtga gcaatctgcc gacgtctggg tgattggcgg
     2041 cgcgcagttg tacgcactgg cggagcctct agccagcacc gccgtggtga ccgaaatcga
     2101 cagggatttt gaaggcaacg cattcgcccc caccctgggt cccgcctgga aggaaacggc
     2161 ccgcgagcgc cacatcgctg ccagtggctt gcctttcagt tttgtcacct acacccgtga
     2221 agcaccatga aacttgccac ctggaacgtg aactctctgg gcgtgcgcct tccccaggtg
     2281 ctggattggc ttgcagccca tccggtcgat gtgctggccc tgcaggaact caaactcacc
     2341 gacgacaagt ttccggtaga tgcctttagc gctgcaggct actcgtccgc gtggtttggg
     2401 cagaaaacct acaacggcgt ggccctgctc tcgcgcacac cggcgaccga tgtggtcaag
     2461 aacattcccg gttttgacga tgacatgtcg cgtgtgatca ccggcaccat cgatggcgtg
     2521 cgggtgattg gcgcctactt ccccaacggg caggagccgg gcagcgacaa gtttgagtac
     2581 aagatggagt ggctcggtgc cttgcgccgc tgggtcaagg aagaactggc acagcacccc
     2641 aggctggtgc tgatgggtga ctacaacatt acctttgacg acgctgatgt ctgggacccc
     2701 gaaggcttgc gcgacaccat ccactgcacc gaggaagagc gctaccacct gcgtgcactg
     2761 atcgcactgg gcctgaccga cagccaccgg ctgtttgcgc acccgcccaa gagttttagc
     2821 tggtgggact accgcgactt cggcttccgg cgcaaccgcg ggctgcgcat c
//
LOCUS       DAOSJM010000324         2868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_30273_length_2868_cov_4.817632, whole genome shotgun sequence.
ACCESSION   DAOSJM010000324 DAOSJM010000000
VERSION     DAOSJM010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30273_length_2868_cov_4.817632"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PKX82_10670"
     CDS             complement(<1..650)
                     /locus_tag="PKX82_10670"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOF52762.1"
                     /translation="MIAACANTAGASWPFDYIFHRGEPLWWLQVAALAALCWGLTRTG
                     SVRLAAMLGLLFASVWLSTTFWWLYVSMHTYAGLHGGLAAIAVVGLAAALGAYYAAAC
                     AIFKKLSQGSGPAAALIFAAAWTCAEMARGTWLTGFGWGAVGYAHVEGPLAFYIPWLG
                     AYGVGALAAWLSATVVHAGARRVGQLAAAAAVLCAGILGPQLLGAWSQPAGTLQVTL"
     gene            complement(718..1587)
                     /locus_tag="PKX82_10675"
     CDS             complement(718..1587)
                     /locus_tag="PKX82_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOF52763.1"
                     /translation="MSDPHPARLSDKEDKRTFLQKLAEFIHPGPDSTDELIETLAEAE
                     DNKIIGTESRLMLEGVIRMADMTAGDVMVPATRMDLINIQAPMDDVLYAVIDTAHSRF
                     PVYEGERENIIGILMAKDLLKLQRAPELNIRALLRPAVFVPETKGLNDLLRDFRGNRN
                     HLAIVIDEFGRVAGLITIEDVLEQIVGEIEDEFDIAEDEGDIFALADRTFRVSGDTPI
                     ERVNDAFSVALNAADPEDQFDTIGGLIAHEMGHVPKRGERHTLGGLDFVVLHTKGGAV
                     KWFKVSPTPTAAP"
     gene            complement(1671..2405)
                     /locus_tag="PKX82_10680"
     CDS             complement(1671..2405)
                     /locus_tag="PKX82_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF52764.1"
                     /translation="MVNPIRLIKASIDTGRDLLRPPTAGDNPDTADAPRAPVLVPIRS
                     LGANHRSRIADHLLSLDDNDRYLRFGYLAHDAQIQKYVDGLDFERDDIFGIYNRKLEL
                     LAVAHLAYAKAHGYEKCAEFGVSVVPAARGRGYGARLFERAAMHSSNEGVRMMFIHAL
                     SENAPMLKIARNAGAHVERDGSESEAYLMLPDASLDSRMTEMLEERYAQTDYNLKVQA
                     KQFWSVLGELQEIRRNAIEAQKRSAS"
     gene            2525..>2868
                     /locus_tag="PKX82_10685"
     CDS             2525..>2868
                     /locus_tag="PKX82_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52765.1"
                     /translation="MDAAETIRNSVARVTALRRAAAAHDDLQAANRAVKRLQARRFAG
                     TYADLLESSEYSGAARFFLDELYSDKDYSLRDAQFSRIAGALQTFFPEQVVATAVALA
                     ELHALTEELDQE"
BASE COUNT          582 a          867 c          845 g          574 t
ORIGIN      
        1 agggtgactt gcagcgtacc cgccggctgt gaccaggctc ccagtaactg aggccccagg
       61 attcctgcac agagcacggc ggcagcggcg gcaagctggc caacccggcg cgcgccggca
      121 tgcaccaccg tggccgacag ccacgctgcc aatgcgccaa ccccataggc acccaaccag
      181 gggatgtaga aagccagtgg accctccaca tgggcatagc ccactgcgcc ccagccgaat
      241 ccggttaacc aggtgcctcg ggccatttct gcgcaggtcc acgcggccgc aaaaatcagg
      301 gcagctgcgg gcccactccc ctgggacagt tttttgaaga tcgcgcacgc cgccgcgtag
      361 tacgcaccca gcgccgcagc aagccccaca acggcgatgg ctgcgagccc tccgtgcaat
      421 ccggcatagg tgtgcatgga cacatacagc caccagaacg ttgtgctcag ccagacactg
      481 gcaaacagca ggcccagcat cgcagccagg cgcacgctgc ccgtgcgtgt gaggccccag
      541 cacagagccg caagcgcggc gacttgcagc caccagaggg gctctccacg gtgaaaaata
      601 tagtcaaatg gccagctagc ccccgccgta tttgcgcaag cagctatcat tacgatagca
      661 atcagaaggc cgggagatga aacgcctgcg ggtgacgctc ggtatatcac catgcactca
      721 aggcgcagcg gttggtgtcg gggagacctt gaaccatttc acggcgccgc ctttggtgtg
      781 cagcacaaca aagtccagcc cacccagggt atggcgctcg ccccgtttgg gtacgtgccc
      841 catttcatgg gcgatgaggc cgccgatggt gtcgaattgg tcttcagggt cagcggcatt
      901 caacgcgaca gaaaacgcat cattcacgcg ctcaatcggc gtgtctccgc tgacccggaa
      961 ggtccggtcg gccagcgcga agatgtcgcc ctcatcctcg gcaatatcga actcgtcttc
     1021 gatttcaccc acgatctgct ccagcacatc ttcaatcgtg atcagtcccg ccacgcgtcc
     1081 aaattcatcg atgacgatgg ccaggtggtt gcggttgccg cggaagtcgc gcagcaggtc
     1141 gttgagtccc ttggtctccg gcacaaacac ggccgggcgg agcagtgcgc ggatgttgag
     1201 ttcgggagcg cgttgcagct tgagcagatc cttggccatc aggatgccaa tgatgttttc
     1261 ccgctctccc tcatacaccg gaaagcgcga gtgggcggta tcgatcacgg catacagcac
     1321 atcgtccatc ggcgcctgga tgttgatcag gtccatcctg gtcgccggca ccatgacatc
     1381 gccagcggtc atgtcggcca tgcgaatgac gccttccaac atcagccgcg attcggtacc
     1441 gatgattttg ttgtcttccg cctcggccag cgtctcgatc agttcatcgg tcgagtcagg
     1501 gcccgggtgg atgaactcgg ccagcttttg caggaaggtg cgcttgtctt ccttgtcgga
     1561 caagcgcgcg ggatgtggat cggacactga agtagcgcaa tatgcggttg ttgcggaacg
     1621 ccaaggatag cggatttgcc ttccaactcc gttaccgccg caatttgcga tcaggaagcg
     1681 ctgcgctttt gtgcctcgat ggcgttgcgg cgtatttcct gcaattcacc gagcacgctc
     1741 cagaactgct tggcctgaac cttcaggttg taatccgtct gggcatagcg ttcttccagc
     1801 atttcggtca tgcggctgtc cagcgaggca tcgggcagca tcaggtaggc ctcagactcg
     1861 gagccatcgc gctctacgtg cgcacccgcg ttgcgggcaa ttttgagcat gggcgcgttc
     1921 tcgctcagcg catggatgaa catcatgcgc acgccctcgt tgctggagtg catggccgca
     1981 cgctcaaaca ggcgggcacc gtaaccccta ccccgcgcag cgggcaccac ggacacaccg
     2041 aattcggcac acttctcgta cccatgtgct ttggcatacg ccaagtgggc cactgcgagc
     2101 agttcaagct ttcggttgta aataccaaaa atatcgtccc gctcgaagtc gaggccatcg
     2161 acgtacttct ggatctgcgc atcatgggcg aggtagccaa aacgcaggta tcgatcgttg
     2221 tcatccaaag aaagcaggtg gtctgcgatc ctggaacggt ggttcgcacc cagcgagcgg
     2281 atgggaacca gcacaggtgc gcgcggcgca tctgccgtgt cagggttgtc tcccgccgtc
     2341 ggggggcgca atagatcgcg ccccgtgtcg atggaagctt tgatcagtcg gattggatta
     2401 accatgggtt gaatataagg gttttcccta tatttggttg tctattcggt gactgtttcg
     2461 tggaataggg actctaatta cgcctaagtt agcgcttcct tgcatggata cacttgtctg
     2521 cacaatggat gctgctgaaa ctattcgcaa ttccgtggcc cgggttacag cgcttcggcg
     2581 cgctgcagcg gcacacgatg accttcaagc cgccaacagg gcggtaaaaa gactccaggc
     2641 ccgtcgattt gcggggacgt atgccgattt gctggagtcc tccgagtatt cgggggcagc
     2701 ccgcttcttt ctggacgagc tgtacagcga caaggattac agcctgcgcg atgcacagtt
     2761 ctcccggata gcgggcgcac tacagacatt ttttcccgag caggtcgtgg caactgccgt
     2821 tgcattggcg gagttgcatg ccctgacgga agaattggac caggaaat
//
LOCUS       DAOSJM010000325         2856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_30454_length_2856_cov_5.137451, whole genome shotgun sequence.
ACCESSION   DAOSJM010000325 DAOSJM010000000
VERSION     DAOSJM010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30454_length_2856_cov_5.137451"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1169)
                     /locus_tag="PKX82_10690"
     CDS             complement(210..1169)
                     /locus_tag="PKX82_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HOF52766.1"
                     /translation="MKQASLKLNLNVKKTRKQVFLEQMEQVVPWAALVERIAPYYPEG
                     KTGRPPFSLQTMLRVHFMQQWFTLSDPGMEEAFFDTPLYREFAQLEEFGRLPDESTIL
                     RFRHRLEKNKLAEQILDVVNEILMQRGLLLKTGTVVDATLIAAPSSTKNKDHKRDPEM
                     HSSKKGEQMYFGMKGHIGTDAESGLVHTVRGTSGNVSDVVEGNSLLHGEETHAFGDAG
                     YQGITKRPDANADVTWHIAMRPGKRRALNKENAVDAMIDKVEKIKAGIRAKVEHPFRV
                     IKRQFGFVKVRYRGLKKNTAQLVTLFALSNLWMVRGKLMGAQG"
     gene            1309..2205
                     /locus_tag="PKX82_10695"
     CDS             1309..2205
                     /locus_tag="PKX82_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52767.1"
                     /translation="MSTALSSSPSIAPSRRKPAASLLAGDSVVYAVLALLVWGTWKIS
                     GMGLFKAGDDVGYWIGVAGGVMMLLLFSYPLRKYFRFAHGWGKVKWWFWIHMVLGVGG
                     PLLILLHSTFRIGSLNAGVALYSMLIVAGSGVVGRFLYSRVNRGLLGELTGLKALQAR
                     AGLDQEQVRSRLAFAPEVEARLKDFEQTELQAHMGWWTHLRRVFWLPVQQWMVYRECV
                     AKLQDPIRKLARHGNWSDQDLARREKQSRKMIRRYLTAVVGVAQYTAYERLFSLWHIA
                     HIPFVYLLVISAIVHVVAVHAY"
     gene            2266..>2856
                     /locus_tag="PKX82_10700"
     CDS             2266..>2856
                     /locus_tag="PKX82_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOF52768.1"
                     /translation="MRWLLLGWLCWAPVAFGQGIESIMAPGKVIEGHAKVEDDCKQCH
                     VKFDRKAQDGLCTACHKDVGADVRNKAGFHGRLSDATCRSCHTEHKGRSARIVELDKK
                     KFDHAKTDFSLKGKHGKTECDKCHIAGKRYREAALECNACHKKDDVHKASLGPKCADC
                     HTENNWKEARFDHDGTKFPLTGKHVDVKCVHCHKNNN"
BASE COUNT          577 a          781 c          821 g          677 t
ORIGIN      
        1 ggtgctttgt cgtcggcagc gtgggccacg cccattgcca gtgtcaaacc caaggatggt
       61 ctgaataact catcgccacg tgctgtagcg agtcgttaat tttcaaatgg cgggatttca
      121 cgcgcaacct ttcaaagttg cgccgctttc tgcacccatt gggctacttt tatccccttg
      181 tggggcattt ctccggattc cgggcgcact catccctgcg ctcccatcag tttgccgcgc
      241 accatccaca aattcgacag cgcaaacagt gtgacgagtt gggcggtgtt cttcttcaag
      301 ccgcggtagc gcactttcac aaatccgaac tggcgcttga tgacccgaaa tgggtgttcc
      361 accttggccc gaatacctgc cttgatcttc tcgaccttgt cgatcatggc atccactgcg
      421 ttttccttgt tcaatgcacg gcgcttgccc ggacgcatgg ctatatgcca ggtaacgtcg
      481 gcattggcat cagggcgttt tgtaatgcct tggtatccag catctccaaa cgcgtgtgtc
      541 tcttcgccat gcaacaaact gttgccctca acgacgtcac tcacattgcc tgaggtgcca
      601 cgaaccgtgt gcacgagacc cgactcagca tctgtgccaa tgtgcccctt cattccgaaa
      661 tacatttgct cgcccttttt gctcgaatgc atttccggat cacgcttgtg gtccttgttt
      721 ttggtcgatg agggagcagc aatcagggtg gcgtccacca ccgtgccggt cttgagcagc
      781 agtccgcgct gcatcagaat ttcattcacg acgtcaagga tttgctcggc caatttgttc
      841 ttctccaacc ggtggcggaa acgcaagatg gtgctctcat cgggcaggcg accaaactcc
      901 tcaagctggg caaactcccg atacagcggg gtgtcaaaga aggcttcctc catacccggg
      961 tcggacagcg tgaaccactg ctgcatgaag tgcacgcgca acatggtctg cagagaaaac
     1021 ggtggccgcc cggtcttgcc ttccgggtaa tacggcgcaa tgcgttcgac cagagcagcc
     1081 cacggaacca cctgctccat ttgttcgagg aacacttgct tgcgcgtctt tttgacgttc
     1141 agattgagct tcaggctggc ttgtttcatg gaggatattt tctcaagcag ggcttacgtc
     1201 aagcacacag tcaagggagt tatgcagagt gtccttaaga aatcagtaat ccgttttcgg
     1261 tacatttggg aggttgggct acgactgtca ccgtagccgt cttttcctat gagtactgct
     1321 ttaagttcat ctccttccat tgctccatcc cggcgcaaac ccgccgccag tttgctggct
     1381 ggcgattctg tggtgtacgc cgtattggcc ttgctggtct ggggcacctg gaagatcagc
     1441 ggcatgggtc tgttcaaggc aggcgacgat gtgggctact ggattggtgt ggctggtggc
     1501 gtcatgatgc tgttgctctt cagctaccct ttgcgcaagt atttccgttt tgcacacggt
     1561 tggggcaagg tgaaatggtg gttctggatc cacatggttt tgggtgtggg tggtcctctg
     1621 ttgatccttc tgcactccac attccggatc ggctcactca atgcaggggt ggcgctgtac
     1681 agcatgctga tcgtggctgg cagcggcgtg gtgggacggt ttctctattc gcgggtaaat
     1741 cgcggcctgc tcggtgagct caccggactc aaggcattgc aagcccgtgc ggggctggac
     1801 caggagcaag ttcgctcacg tctggctttt gcacccgagg tcgaagcccg gctgaaagac
     1861 tttgagcaga ccgaactgca ggcgcatatg ggctggtgga cccatctccg acgcgtattc
     1921 tggttgcccg tgcagcagtg gatggtgtac cgtgaatgcg ttgccaaact gcaggatccc
     1981 atacgcaagt tggctcggca cggaaactgg agcgaccaag atttggcgcg gcgggagaag
     2041 caatcccgga agatgattcg tcgttacctc accgcggtag tgggtgtggc gcagtacacg
     2101 gcctatgagc gcctgttttc gctgtggcac atcgcccata tcccattcgt ctatctgctg
     2161 gtgatcagcg ccatcgtcca tgtggtggcg gtgcacgctt attgaccgtg tcatgtgatg
     2221 ccatgccaac cacttctgtc ctgctttgcg acgtggagcc agtgtttgcg ctggttgttg
     2281 ctgggttggc tatgttgggc accggtggca ttcgggcagg gtatcgagtc catcatggca
     2341 cccggtaagg tcatcgaggg tcatgccaaa gtcgaggacg actgcaagca gtgccacgtc
     2401 aaatttgacc ggaaagcgca ggatggtttg tgcacggcat gccacaagga tgtgggtgct
     2461 gatgtgcgca acaaggcagg attccacggt cgtttaagcg acgccacctg tcgcagttgc
     2521 catacggagc acaagggccg cagcgcacgc atcgtggagc tggacaagaa gaaatttgac
     2581 cacgccaaga ctgacttttc cctcaagggc aagcacggca agacggagtg cgataagtgc
     2641 catattgcgg gcaagcgata ccgggaggca gcgctggagt gcaacgcatg ccacaagaaa
     2701 gatgatgtgc acaaggcatc cctcggcccc aagtgcgcgg attgccatac cgaaaacaac
     2761 tggaaagaag cgcgcttcga ccacgacggc accaaatttc cgctgaccgg caagcacgtc
     2821 gatgtgaaat gcgtgcattg ccacaagaac aacaac
//
LOCUS       DAOSJM010000326         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_30872_length_2827_cov_4.811328, whole genome shotgun sequence.
ACCESSION   DAOSJM010000326 DAOSJM010000000
VERSION     DAOSJM010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30872_length_2827_cov_4.811328"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PKX82_10705"
     CDS             <1..404
                     /locus_tag="PKX82_10705"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOF52769.1"
                     /translation="IFHWGYRYGAQLLRRKFFARMLPEARLQSMQATMTHYGPAYIFV
                     VRFLPGIRTPLFLVAGMSRMPYRHLFVYDGLAAAVELPLLVYGVRYVGGRWREILASM
                     QQLQDFLLPAAVLAVLALWLYRRRVTKGNSV"
     gene            401..1864
                     /locus_tag="PKX82_10710"
     CDS             401..1864
                     /locus_tag="PKX82_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOF52770.1"
                     /translation="MKPMIKTALSRKGRSSSAVLGDLQAYGAHDPDYKSGRLWSLVYY
                     LDEEYAAFQAQAYQAFSSANGLNPTAFKSLKQFETEIIAGVSEMLHGTPEVCGVVTSG
                     GTESCLLAVKTYRDMARARGVKRPEMVLPVTAHVAWFKASEYFDVKVRLLPLDKNLRA
                     DVGKLPRLVNRNTVMILGSAPEYPHGTIDPIEAFGAIAQKRGVPLHVDACVGGFILPF
                     MEMNGQELPLWDYRVPGVTSISADIHKYGFASKGASTITYRTLDYLKHQMFVQQDWPG
                     GVFASPALLGTRPGGAYAAAWAAMQYFGKTGYRDLARRTTQAFTAMRNGVDAMPELYV
                     MGDPSGPLLAYGSRDPEVNIYAVGDQMDAKGWKVNRLQNPDGLHAMITAGHREVVDDY
                     LRDLREAVATVKADPTLASAGSAATYGMMSHLPMRGMVRQRVLDMFAQMYQAGGQNID
                     MHATTAPGQGLAGRVQALAEKAAHWYVERAQRKAAGR"
     gene            2229..>2827
                     /locus_tag="PKX82_10715"
     CDS             2229..>2827
                     /locus_tag="PKX82_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF52771.1"
                     /translation="MDAAVPSRGGIVVSWANHQDEVRAAQRLRYDVFAGEMGARLTTP
                     IAGHDIDLFDNYCEHLLVRDQDTQQVIGTYRVLTPAQALRVGSTYSDTEFDLTRLRSL
                     RERMVELGRSCVHPDHRHGGVIMALWGALGEFMVRNQLDTMIGCASIPMLHNGVVSGD
                     IAASIWRKVQATHLAPIEYHVRPRLPLPVEQLDGSLDVEP"
BASE COUNT          554 a          811 c          853 g          609 t
ORIGIN      
        1 tgatctttca ttggggctac cgttatggag cacagctgct gcgcaggaag ttcttcgcgc
       61 gcatgctgcc agaggcacga ctgcagtcca tgcaagccac catgactcac tatggccccg
      121 cctacatctt cgtggtgcgc tttttgcccg gcattcgcac gccccttttt ctggtggcag
      181 gcatgtcgcg catgccctac cggcatttgt ttgtatatga cggcctggct gctgcggtcg
      241 agttacctct gctggtctat ggcgtgcgct acgtcggagg acgatggcgg gaaattctgg
      301 catcaatgca gcaattgcaa gacttcttgt tgcctgcagc cgtcctggcg gttctggcgc
      361 tgtggctata tcgccggcgc gtaacaaaag gaaactccgt atgaaaccca tgatcaaaac
      421 agcgttgtcg cgcaagggcc gttcttcatc ggctgtgctg ggagatctgc aagcctatgg
      481 cgcgcacgat ccggactaca aatccggccg tttgtggagt ctggtgtatt acctggatga
      541 agagtacgca gcctttcagg cccaggccta tcaggcattt tcgtccgcca atgggctgaa
      601 cccgaccgct ttcaaaagcc tcaagcagtt cgagaccgag atcatcgcgg gcgtatccga
      661 gatgctgcac ggcactcccg aggtgtgcgg cgttgtaacg tctggaggta ctgagagttg
      721 ccttctggca gtcaaaactt accgcgatat ggcacgtgcg cgaggcgtga agcgtcctga
      781 gatggttttg cctgtaacag cccacgtcgc atggttcaag gcatccgaat acttcgacgt
      841 gaaggtgcgc ctgttgccac tggacaagaa tctgcgtgcc gatgtaggca aactgccgcg
      901 cctggtcaac cgaaacacgg tgatgattct gggttcggca cctgaatacc cgcacggcac
      961 catcgatccg attgaggcat ttggtgctat tgcacaaaaa cgcggtgtac cgttgcatgt
     1021 ggatgcctgt gtgggtgggt tcatcctgcc tttcatggaa atgaatggcc aagagttgcc
     1081 gctgtgggat taccgcgtac ccggggtgac atcgatttca gcagacattc acaagtacgg
     1141 ctttgcgtcc aagggggcct ccaccatcac ctatcgcact ttggattacc tcaaacacca
     1201 aatgtttgtg caacaggact ggccaggtgg cgtttttgca tctcccgcgt tgttggggac
     1261 acgtcctggc ggtgcttacg ctgcagcctg ggcggccatg cagtattttg gcaagacggg
     1321 ctatcgtgat cttgcgcggc gaacgacaca ggcattcacc gctatgcgca acggtgttga
     1381 tgccatgccg gaactgtatg tcatgggcga tcccagtggg ccgctcttgg cttatggttc
     1441 acgcgatccc gaggtcaaca tttatgccgt gggcgaccag atggatgcca aaggctggaa
     1501 ggtcaaccgt ttgcagaacc cggacggatt gcacgccatg attactgccg gtcatcggga
     1561 ggtcgtggac gactacctgc gagatctgcg cgaagcggta gccacggtca aggccgatcc
     1621 gacgctggcc agtgcgggca gtgccgcaac ctatggaatg atgagtcatc tgcccatgcg
     1681 tggcatggtg cgccagagag tgttggacat gtttgcacag atgtaccagg cgggcggaca
     1741 aaacatagat atgcatgcaa caaccgcgcc tggacaaggt ctggctgggc gagtgcaggc
     1801 cctggcggaa aaagcagccc attggtatgt ggagcgggca cagcgcaaag ctgcaggtcg
     1861 ttgagacccg gcaagctgac agcctatgga tggaggctgg attgcgctgg ggtgcttcat
     1921 cctgataggt tttaggatag aggcgatcac cggctttggc ggaacggttg ttgcattggc
     1981 gttgggcgcg ttgatgctgc ccataccagt gttgctgccc attctggtac ccccgtctct
     2041 ggaggttgtt cacattctgg gcgtccgtat gtcacaaaaa cttcatgccg ttgccacgaa
     2101 gttgacacca cccaaattca gactctgctt tcagtacaac accattgaac tgacaggaga
     2161 tccagatgct caacatcctc cccgcatccc acgctgccac cggcagtatc gtcgtcaata
     2221 cccctgcgat ggacgctgca gtgccgtccc gtggtggcat cgtcgtgtcc tgggccaacc
     2281 accaggacga ggtccgcgcg gcccagcgcc tgcgctatga cgtgtttgct ggtgagatgg
     2341 gcgcccgcct gacaaccccc attgccggcc acgacatcga cctgtttgac aactactgcg
     2401 agcacctgct ggtccgtgac caggacaccc agcaggtgat tggcacctac cgcgtactca
     2461 ccccggcaca agcgctgcgc gtgggcagca cctacagcga taccgagttt gacctgaccc
     2521 gcctgcgctc cctgcgcgag cgcatggtgg agcttggtcg cagctgcgta catccggacc
     2581 accgccacgg tggcgtgatc atggcgctgt ggggcgcgct gggtgagttc atggtgcgca
     2641 accagctcga caccatgatt ggctgcgcca gcatcccgat gctgcacaac ggtgtggtca
     2701 gcggtgacat cgcggccagc atctggcgca aggtgcaggc gacccacctg gcgcccatcg
     2761 aataccacgt gcgcccacgc ctgccgctgc cggtggagca actggatggt tccctggatg
     2821 tggagcc
//
LOCUS       DAOSJM010000327         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_31104_length_2811_cov_3.671988, whole genome shotgun sequence.
ACCESSION   DAOSJM010000327 DAOSJM010000000
VERSION     DAOSJM010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31104_length_2811_cov_3.671988"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PKX82_10720"
     CDS             <1..820
                     /locus_tag="PKX82_10720"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HOF52772.1"
                     /translation="HPTVNQIVARADAVFIAGGDQSDYVKYWKNTPLDWTLQSLMNRK
                     VPIGGTSAGLAVLGQFDFAALRGGVTSAQALSNPFNKYMSVDPTPQTLTPTSSSAPGG
                     AFLAPPVLFNTILDAHVDERDRMGRLVTFVSRLITPYYGAGCSGGILVPREAQSLSPT
                     PYGDIVARGIGVGVEAALLVSGNGAASSPITATLVTNPTTTTRSAAYFVLPQNYPGQC
                     TSGKALSGVTATVVKVDRLNPTFNLSTWSGGYAPYTLTVTNGVMTVSGNGGDPY"
     gene            complement(896..1723)
                     /locus_tag="PKX82_10725"
     CDS             complement(896..1723)
                     /locus_tag="PKX82_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOF52773.1"
                     /translation="MSNAVSVTTRGSITVVTLNRPDVRNAVDADTAQLLYDAFVSFDE
                     DPNSRVAVFHGAHGHFCAGWDLQAGARLAQQGASTSDAFSRLNFSPVDYAQAGNSSAG
                     PQGPMGPSRLILSKPVIAAVSGAAVAGGMELALWCDMRVMEDDAYFGVFCRRFGVPLI
                     DGGTVRLPRLIGMGHAMDLILTGRKVEAPEALAMGLCNRVVPKGTALEAAVALAEQLA
                     GFPQQTMLADRASAYAQWDMDLPHALHQEWERGKACIASGLEGAGRFAAGAGRHGKF"
     gene            1830..2726
                     /locus_tag="PKX82_10730"
     CDS             1830..2726
                     /locus_tag="PKX82_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOF52774.1"
                     /translation="MQFLQLVISGVAQGCIYGLIALGFVLIYKATETVSFAQGELMML
                     GAFCGLVAMTLLGFPYWLAVLSSIAAMAVFGIVLERLVIRPILGQPAFSIVMLTIGIG
                     YVARGLITMIPGIGTDTQALPVPYKDEIWKIGGNEMGVGALVLNVEHMAVIGATAVLC
                     VLLFALFRYSKLGIAMQAASQNQLAAYYMGIPVQRLNGIAWGLAAAVAAIAGLLLAPI
                     TFVHANMGFIGLKAFPAAVVGGFGSLPGAIVGGLLIGIVESLSGFYLPDGFKDTAAYI
                     VVLLMLMVKPNGLFGEKLRKKV"
BASE COUNT          522 a          880 c          849 g          560 t
ORIGIN      
        1 ccaccctacg gtgaaccaga tcgtggcgcg ggccgatgct gtcttcattg ccggcgggga
       61 ccagagcgac tacgtcaagt actggaagaa caccccactc gactggaccc tgcaatccct
      121 gatgaaccgc aaggtaccca ttggcggcac cagtgccggt ctggccgtgt tggggcaatt
      181 tgactttgcc gcgctgcgtg gcggcgtaac gtcggcgcag gctctgtcca acccgttcaa
      241 caaatacatg agcgtggacc ccacccccca gaccctcaca ccaacctcat ccagcgctcc
      301 gggaggcgcc ttccttgcgc cacccgtcct gttcaacacc attctggacg cgcacgtgga
      361 tgagcgcgac cgcatgggcc ggttggtgac cttcgtatcc cgcttgatca cgccctatta
      421 cggtgccggt tgcagtggcg gcattctggt acccagggaa gcccaaagcc tgtcgcccac
      481 accctatggc gacatcgtgg cgcgcggcat tggggtgggt gtcgaggctg ccttgctcgt
      541 gtctggcaat ggtgccgcca gtagcccgat cacggcgacg ttggtcacca acccgaccac
      601 cacgacacgc agcgcagctt attttgtgct gccgcaaaac tacccgggcc agtgcaccag
      661 tggcaaggca ctttctggcg tgaccgctac cgtcgtcaag gtggaccgac tgaacccgac
      721 cttcaacctg tcgacctggt ctggcggata tgccccgtat acgctgaccg tcaccaatgg
      781 cgtgatgacc gtatcgggta atggcggcga tccgtattga tacggagtca cgccaggaag
      841 ctataaaata tatagcgcac tgcgcatatc tcacgggggc cagaggccaa tttagctaaa
      901 atttaccgtg ccgccccgcg ccggcggcaa agcgccccgc cccttccagc cccgacgcaa
      961 tgcaggcttt gccgcgctcc cactcctggt gcagtgcatg gggcagatcc atatcccact
     1021 gtgcataggc actggcgcgg tcggccagca tggtctgctg cggaaagccg gccagttgct
     1081 ccgccagtgc caccgctgcc tccagcgccg tgcctttggg caccacgcgg ttgcatagcc
     1141 ccatggccag cgcttcgggc gcctcgacct tgcgcccggt gaggatcagg tccatggcgt
     1201 ggcccatgcc gatcaggcgc ggcaggcgca ccgtgccacc gtcaatgagc ggcacaccaa
     1261 agcggcggca gaacacgccg aagtaggcat cgtcttccat cacacgcata tcgcaccaca
     1321 gcgccagctc catgccgccc gccaccgcgg ccccgctgac tgcggcaatg acgggcttgc
     1381 tgagtatcag acgcgatggg cccatggggc cttgtggccc tgcgctgctg ttgcccgcct
     1441 gcgcatagtc gacaggagaa aagttcaacc tggaaaacgc atcgctggtg ctggcaccct
     1501 gctgggccag ccgggcgccg gcctgcaggt cccagccggc acagaagtga ccatgggctc
     1561 cgtggaaaac agccacacgt gaattggggt cttcatcgaa tgacacgaat gcgtcgtaca
     1621 gcagctgcgc ggtatccgcg tcgacagcgt tgcgaacatc gggcctgttc agggttacta
     1681 ctgtgatgct tccacgcgtt gtcactgaaa ctgcattgct catgcggttc attacatagt
     1741 cccccgagtt acaggggaat cagtgacaac cctatcaacg atcgccgcaa tgcccgatat
     1801 aaccgggcca cagaacaaca ggagacaacg tgcagtttct tcaactcgtg atcagcggag
     1861 ttgcgcaggg gtgcatctac ggattgattg cgctgggctt tgtgctcatc tacaaggcga
     1921 ccgagacggt cagcttcgcc cagggcgaac tgatgatgct gggcgcattc tgcggactgg
     1981 tcgccatgac cctgctgggc tttccctact ggctggcggt gctgagttcc attgcagcca
     2041 tggcggtgtt tggcattgtg ctggagcgcc tggtgattcg ccccattttg gggcagcccg
     2101 cgttttccat tgtgatgctg accatcggca ttggctatgt ggcgcgcggc ctgatcacca
     2161 tgatccccgg tatcggtacc gatacgcagg cgctgcccgt gccctacaag gatgagatct
     2221 ggaagatcgg tggcaacgaa atgggcgtgg gtgcactggt gctcaacgtg gaacacatgg
     2281 cggtgattgg tgccacggcc gtgctgtgcg tgctgttgtt cgcactgttc cgctacagca
     2341 agctgggtat tgcgatgcag gctgcgtcgc aaaaccagct ggcggcgtat tacatgggca
     2401 ttccggtgca gcggctcaac ggcattgcct ggggcctggc cgctgcggtg gcagccattg
     2461 ccgggctgct gctggcgccc atcacctttg tgcacgccaa catgggcttc attggattga
     2521 aggcatttcc ggcagccgta gtcgggggct ttggcagcct gccaggcgcc atcgtgggtg
     2581 gcctgctgat tggcattgtg gagtcgctct cgggcttcta cctgcccgat ggcttcaaag
     2641 acaccgccgc gtacatcgtg gtgctgctga tgctgatggt caagcccaac ggcttgtttg
     2701 gcgaaaaact gcgcaagaaa gtctgacatg cgtttcatct tcaagaccga ctacgcgcag
     2761 gacatcaagc tcgccaaaca tggcggccac ttgttctggt acagcgcatt g
//
LOCUS       DAOSJM010000328         2803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_31216_length_2803_cov_3.866812, whole genome shotgun sequence.
ACCESSION   DAOSJM010000328 DAOSJM010000000
VERSION     DAOSJM010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31216_length_2803_cov_3.866812"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PKX82_10735"
     CDS             complement(<1..454)
                     /locus_tag="PKX82_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52775.1"
                     /translation="MHRQTSQPARKSVVQWTTLCASIAALVAGCATRPPEAPPAPVAA
                     APAPAPYVAPLTAPDKISNATTPRAYRQDAASHLYAHNGKRIYSGKMPPLLYAVGVLQ
                     VEIDGRGNVLNISWMRKPSHAPEVVAEIERSVRQAAPFPAPTKMGRVTY"
     gene            550..1356
                     /gene="xdhC"
                     /locus_tag="PKX82_10740"
     CDS             550..1356
                     /gene="xdhC"
                     /locus_tag="PKX82_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HOF52776.1"
                     /translation="MVNVKSQFLQRLGSESAVLVTVQAFRGSVPRETGAWMAVLADAT
                     LGTVGGGHLELDAIAHARTLLASGAGDEVVRYALGPSLGQCCGGEVHLRYKWVQAQDP
                     AALKTSLDSATRPVALFGGGHVGRALVQVLGNLPFTVRWVDSRDEIFPVDVPDNVQCE
                     HSDPVQAAVADLVPGSRVVVMSFSHAEDLDVVAACLARQRLRGDLPYVGLIGSQTKWA
                     AFRHRLEARGFSGAEIAHVTCPIGVPGIRDKRPEVIAVGVAAQLLQVPVE"
     gene            1399..2619
                     /locus_tag="PKX82_10745"
     CDS             1399..2619
                     /locus_tag="PKX82_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HOF52777.1"
                     /translation="MESYLLDWANLLLRWVHVITAIAWVGSSFYFVFLDSSLTPPVDE
                     DLKKQGVSGELWALHGGGFYHPVKFAGAPPQLPGHLHWFYWESYSTWLTGFSLFTVSY
                     LWSAGTYLIDKSVMAWTPAAAVAVALSFLVVFWLAYDAICRIFGQRKHGDAIVGALVL
                     VLVCVASYLACHWFAGRAAFLLVGAMIATAMSANVFFWIIPGQKTVVADIKAGRPVDP
                     IHGKRGKQRSVHNTYFTLPVLFAMLSNHYSFTYSHPQNWLVLILMMLAGAAIRQFFVL
                     RHGFKLGRNPHPWPYAAVGVVVILATIVWLKPKIAINSVADGAGNTGASGPIDYKTLQ
                     PVLAQRCYLCHGAEVQMKNVRLDSPQGVATHAQAIYQQVVVSKIMPLNNATQITDAER
                     ALIRRWFESGAAVN"
     gene            2651..>2803
                     /locus_tag="PKX82_10750"
     CDS             2651..>2803
                     /locus_tag="PKX82_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOF52778.1"
                     /translation="MTQSFRYTLLSLRDLVASAGPFFALAIALLVLAYWWLDPNPPQR
                     VTLATGP"
BASE COUNT          452 a          813 c          939 g          599 t
ORIGIN      
        1 tgtaggtcac gcggcccatc ttggtgggtg ccgggaaggg cgcagcctgg cgcacggacc
       61 gctcgatttc tgccaccact tcaggcgcat ggctgggctt gcgcatccag ctgatgttga
      121 gaacattgcc ccggccgtcg atctccacct ggagcacgcc cacggcatac agcaggggcg
      181 gcatcttgcc ggaataaatg cgctttccat tgtgtgcata caggtgcgat gccgcgtcct
      241 ggcggtaggc gcgcggtgtc gtcgcgttgg aaattttgtc gggcgcggtt agcggcgcta
      301 catagggagc cggtgcgggg gctgccgcca caggtgccgg gggcgcttcg ggcggacggg
      361 ttgcacatcc ggcaacgagg gcggcaatgc tggcgcaaag ggtcgtccat tgcacaacgc
      421 ttttgcgggc tggctgggat gtctgacggt gcatggtctg tctcctgggc tgttttttgc
      481 atgctggaag gcatactgtg cccgaacttc ggggcgccgt gccagcctgg cttttgataa
      541 aggtatccaa ttgtgaatgt gaaatcgcag tttttgcaac gtttgggttc ggaaagtgcc
      601 gttttggtca ccgtgcaagc ctttcgcggc tcggtaccgc gcgagacagg tgcctggatg
      661 gctgtgctgg ccgatgccac cctgggaacg gtggggggtg gccatctgga actggatgcc
      721 attgcccatg cgcgcacctt gctggcatcc ggtgctggtg atgaggtggt gcgctacgcg
      781 ctgggtccga gcctgggcca atgttgcggt ggcgaagtgc atttgcgtta caaatgggta
      841 caggcacagg accctgccgc actgaaaacc agcctggaca gcgccacgcg gccagtggcg
      901 ctgtttggtg gcgggcatgt ggggcgtgcg ctggtgcagg tgctgggcaa cctgccgttc
      961 accgtgcgct gggtggacag ccgggatgag atattccccg tggacgtgcc cgacaacgtc
     1021 cagtgcgagc attctgatcc ggtgcaggcg gcagtagccg atctggtacc aggctcgcgc
     1081 gtcgtggtca tgagcttcag ccatgcggaa gacctggacg tggtggccgc atgcttggcg
     1141 cgccagcggt tgcgggggga cctgccctat gtgggattga tcgggagcca gaccaagtgg
     1201 gccgcatttc gccaccggct ggaagcccgt ggcttcagcg gcgcagagat cgcccatgtg
     1261 acctgcccga ttggtgtgcc gggcatccgc gacaaacggc cggaagtgat tgccgtaggg
     1321 gtagcggcac aactcttgca ggttccggtt gaatgagaag tgtatacact ttgatccttc
     1381 gctaggagaa cacaccgtat ggaaagctac cttctggact gggccaatct gctgctgcgc
     1441 tgggtccatg tcatcacagc cattgcctgg gtgggttctt cgttctattt tgtctttctg
     1501 gacagcagcc tcacgccgcc ggtggatgaa gacctgaaga agcagggcgt cagcggtgaa
     1561 ctgtgggcct tgcacggcgg tggtttctac cacccggtca agtttgccgg cgcgccgccg
     1621 cagttgccgg gccatctgca ctggttttac tgggaaagct atagcacctg gctgaccggg
     1681 ttctcgctgt tcaccgtgtc ctacctgtgg agcgctggca cttacctgat cgacaagtcc
     1741 gtcatggcct ggacgcctgc ggcggccgtg gccgtggcgc tgtcgttcct ggtggtgttc
     1801 tggctggcct acgatgccat ttgccgcatc tttggccagc gcaagcatgg cgacgccatc
     1861 gtcggtgcgc tggttttggt gctggtgtgt gtggcctcgt acctcgcttg ccactggttt
     1921 gccgggcgcg ccgccttcct gctggtcggc gcgatgatcg ccacggccat gagcgccaat
     1981 gtgtttttct ggatcattcc cggccagaag actgtggtgg ccgacatcaa ggcgggccgg
     2041 ccggtggacc ccatccacgg caagcgcggc aagcagcgca gcgtgcacaa cacctacttc
     2101 acgctgccag tgctgtttgc catgctgagc aaccactaca gcttcaccta cagccaccca
     2161 cagaactggc tggtgctgat cctcatgatg ttggccggcg cggcgattcg ccagttcttc
     2221 gtgttgcgcc acggcttcaa gctcgggcgc aatccgcatc cctggcccta tgcggcggtg
     2281 ggtgtggtgg tcattctggc caccatcgtc tggctcaaac cgaaaattgc tatcaattca
     2341 gtagcggatg gtgcaggtaa tacgggggct agtggcccaa ttgactacaa gacactgcag
     2401 ccggtgctgg cccagcgctg ctacctgtgc cacggtgccg aagtgcagat gaaaaacgtg
     2461 cgtctggact cgccacaggg cgtggccacc cacgcacagg cgatctacca gcaggtggtg
     2521 gtcagcaaga tcatgcccct gaacaacgcc acccagatca ccgatgccga gcgtgcgctg
     2581 atccggcgct ggttcgaaag tggtgccgcg gtgaactgac cgatggctgg ccgcgtgcta
     2641 aggtagtgcg gtgacccaat cttttcgcta caccctgctc tcgctgcgtg acctggtcgc
     2701 gtcggcgggg ccgttttttg cactggccat cgctttgctg gtgctggcgt actggtggct
     2761 ggacccgaat ccaccgcaac gcgtgacgct ggccaccggc ccg
//
LOCUS       DAOSJM010000329         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_31436_length_2787_cov_3.665813, whole genome shotgun sequence.
ACCESSION   DAOSJM010000329 DAOSJM010000000
VERSION     DAOSJM010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31436_length_2787_cov_3.665813"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..2151
                     /locus_tag="PKX82_10755"
     CDS             382..2151
                     /locus_tag="PKX82_10755"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HOF52779.1"
                     /translation="MYYSNGNYEAFARPRKPKGVENKTAWFVGSGLAALAGAAFLIRD
                     GQMPGGQINILEQQQLPGGALDGIKDPEKGFVIRGGREMEDHFECLWDLYRSIPSLEI
                     EGASVLDEFYWLDKDDPNSSLQRATMNRGEDACTDGLFTLSEQAQKEIVKFFLATREE
                     MENKRINEVFGGEFLGSNFWLYWRTMFAFEEWHSALEMKLYLHRFIHQIKGMPDFSTL
                     KFTKYNQYESLVLPLVKWLQDHGVVFHYGTVVTDVDFDIAPGQKQATRIHWRRDAVEG
                     GVDLGADDLMFMTIGSLTENSASGDHYTPANLNLGPAPAWDLWRRIAAKDPAFGHPDV
                     FGAHISETKWESATVTTLDARIPQYIQKIAKRDPFSGKVVTGGIVTAKDSSWLLSWTV
                     NRQPHFKQQPKDQIVVWVYALFVEQPGDYVKKPMQDCSGEEITQEWLYHLGVPVEAIP
                     ELAATGAITIPVMMPYVTAFFMPRQAGDRPDVVPAGAVNFAFIGQFAQSRERDCIFTT
                     EYSVRTPMEAVYTLLGVERGVPEVFNSTYDIRSLLGATGRLRDGKEINVPGPAFVRKL
                     LMNKLDQTQIGALLREFGLVTDD"
     gene            2219..>2787
                     /locus_tag="PKX82_10760"
     CDS             2219..>2787
                     /locus_tag="PKX82_10760"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: protein motif:HMM:NF017873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HOF52780.1"
                     /translation="MKKKEDSSANVYLNGLGLGSIAASMYLIQKADFNPSNIHIFEQM
                     GARTLVGGSCDAAQMTVNDQPAYVMQGSRMFEDKVYPCTKELWSLIPYNDDESCLQDY
                     QKNREECRVHTVVRLLHEHGEKDTGYQLGLNMAEKLKMAKLLATPEFLIPDGAKITDI
                     FGEAFFTSNYWYMWRALFAFQPWHSAVEI"
BASE COUNT          632 a          789 c          797 g          569 t
ORIGIN      
        1 cgcagccaat ggcggcctga acacgaaggg gggaactacg cactgtccag ttaacagctc
       61 ggctttgaat gctagtcaat tgatgcaact ccatctgtgc gcgtgcacac atacatgcca
      121 tcgctctctt ggtcgtagct ttgtgacaac catctcttca acgccgcctg caagcagggc
      181 cttgcgtggt ctaccgcacc gactgccaac gcttgggaga tcacagtggc gggtctggac
      241 acgccatgaa gccacaactt ctgtctgcgc ataggcccta gcccagttca tcacccagcc
      301 cggcccgaat cgctcaaagt agcttttcat gaccctcaat gccgcttcgg ggtggctaca
      361 acgttacaca aggaaaactg catgtattac agcaatggca actacgaagc cttcgcccgt
      421 ccgcgcaaac ccaagggggt tgagaacaaa acagcctggt ttgtcggctc ggggctagcg
      481 gcattggcgg gggcggcctt cctgatccgt gatggtcaga tgccgggcgg gcaaatcaac
      541 attctggagc agcagcagtt acccggtggt gcgctggacg gcatcaaaga tccggaaaaa
      601 ggtttcgtga ttcgtggcgg gcgcgagatg gaagaccact ttgaatgcct gtgggacctg
      661 taccggtcaa ttccatcgct ggagatcgag ggcgccagcg tgctcgacga gttctattgg
      721 ctcgacaagg acgatccgaa ttcttctttg cagcgcgcga ccatgaaccg aggcgaggat
      781 gcgtgtaccg acggtctgtt cacgctgagc gagcaggccc agaaggaaat tgtcaagttc
      841 ttcctggcga cccgcgaaga aatggagaac aaacgcatca acgaggtatt cgggggtgaa
      901 ttcctgggca gtaatttctg gctgtactgg cgcacgatgt ttgccttcga ggagtggcac
      961 tcggcgttgg agatgaagct gtacctgcac cgtttcattc atcaaatcaa aggtatgcct
     1021 gacttctcca cgctgaagtt caccaagtac aaccagtacg aatcgctggt gctgccactg
     1081 gtgaagtggt tgcaggatca tggcgtggtg ttccattacg gaaccgtggt gaccgatgtc
     1141 gatttcgaca tcgcacccgg tcaaaagcag gccacccgca tccactggcg cagggacgca
     1201 gttgaaggcg gtgtcgatct tggggctgac gacctgatgt tcatgaccat cggctcgctc
     1261 accgagaact cagccagtgg tgaccactac acgccagcga atctcaacct tggacctgcg
     1321 ccggcgtggg atctgtggcg gcgcatcgct gcaaaggatc cggcgttcgg gcacccagac
     1381 gtcttcggcg cgcacatctc cgagaccaaa tgggaatcgg ccaccgtcac cacgcttgat
     1441 gcgcgcatcc cgcagtacat ccagaagatc gccaagcgtg atccgttcag cggcaaggtg
     1501 gtgactggcg gcatcgtgac cgccaaggac tcctcctggc tgctcagctg gacggtgaac
     1561 cggcaaccgc acttcaagca gcagcccaag gaccagatcg tggtgtgggt ttacgcgctg
     1621 tttgtcgagc aacccggcga ttacgtgaaa aaaccgatgc aggactgcag cggcgaagag
     1681 attacccagg agtggctcta ccacctgggc gtgccggtcg aggccatccc cgaactggcc
     1741 gccacaggtg ccatcactat cccggtgatg atgccgtacg tgacggcctt cttcatgcca
     1801 cgccaggcgg gtgatcgccc ggatgtcgtg cctgcgggcg cggtcaactt cgccttcatt
     1861 ggccagttcg cgcagtccag ggagcgcgac tgcatcttca ccaccgagta ttcggtgcga
     1921 acaccaatgg aagcggtcta cacactgctc ggcgtcgagc gcggcgtgcc cgaagtcttc
     1981 aactccacct acgacatccg gagtcttctg ggcgcgaccg gccgtctgcg cgatggcaag
     2041 gagatcaatg tcccggggcc tgcattcgtg cgcaagctgc tgatgaacaa gctcgaccag
     2101 acccagattg gcgccctgtt gcgcgaattc ggactggtca cggacgattg accccacttc
     2161 cttcatgtca gtcttgtgat tgatacctag acggtcaata aacaaaaggt acaccaaaat
     2221 gaaaaagaaa gaagactctt ccgccaacgt ttatctcaat ggtctcggct tgggctccat
     2281 tgctgcgtcg atgtatttga tccagaaggc tgacttcaat ccatccaata tccatatctt
     2341 cgagcaaatg ggtgccagga cgctcgtggg aggaagctgt gacgccgcgc agatgaccgt
     2401 gaacgaccag ccggcctacg tgatgcaagg cagtcggatg ttcgaggaca aggtgtatcc
     2461 ctgcaccaag gaactgtggt ccctcattcc gtacaacgac gatgaatcgt gcctgcaaga
     2521 ctatcaaaag aaccgtgaag aatgcagggt tcacacggtc gtccgtcttc tgcatgaaca
     2581 cggtgagaag gacactggat atcaactggg tctgaacatg gcagaaaaac tgaagatggc
     2641 aaaacttctg gccacgcccg aattcttgat tcccgacggt gcaaagatca ccgacatctt
     2701 cggagaggcc tttttcacca gcaattactg gtacatgtgg cgggcacttt ttgctttcca
     2761 gccgtggcat tcagcggtgg aaataaa
//
LOCUS       DAOSJM010000330         2754 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_31910_length_2754_cov_4.736199, whole genome shotgun sequence.
ACCESSION   DAOSJM010000330 DAOSJM010000000
VERSION     DAOSJM010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31910_length_2754_cov_4.736199"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PKX82_10765"
     CDS             complement(<1..643)
                     /locus_tag="PKX82_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOF52781.1"
                     /translation="MPGLLSNVDPDGLLEFSVVYTDRALNHMSQRFQGVMKDISRILK
                     EVYHAPSAVLVPGSGTFGMEAVARQFATGKKALVIRNGWFSYRWTQIFDMGSIPAEST
                     VLKARRVGDSAQAPWIPCPVDEVVAAIRAKKPDVVFAPHVETAAGMILPDDYLRKVAD
                     ATHEVGGLFVLDCIASGAMWVNMENTGVDVLVSAPQKGWSGSPACAMVMLSERA"
     gene            complement(699..1550)
                     /gene="asd"
                     /locus_tag="PKX82_10770"
     CDS             complement(699..1550)
                     /gene="asd"
                     /locus_tag="PKX82_10770"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794428.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOF52782.1"
                     /translation="MSDTLAVLPQYLLPKKLLTALAGKLAGAHGGAATTALIRRFVKR
                     YNVNMAEAANPDIASYPTFNDFFTRPLRAGVRPLAAADFISPVDGAISQFGAIDKDQI
                     FQAKGHSYSTTALVGGDAALGATFNGGSFATLYLSPRDYHRIHMPCDGVLRRMVYVPG
                     DLFSVNPTTARGVPGLFARNERVVCVFDSAHGPFVLTLVGATIVGSMATVWHGLVNPP
                     RLPQVTEWHYDAGKVVLRKGEEMGRFLLGSTVVMLFPPSTLEFNPQWAPAKPIAMGEA
                     MGEHLSR"
     gene            1623..2495
                     /locus_tag="PKX82_10775"
     CDS             1623..2495
                     /locus_tag="PKX82_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOF52783.1"
                     /translation="MTAARIPPIQCLLTFEALARLRSVTQASEELCVTPSAVSHRVKQ
                     LEQIIGTKLFGRADFSLTTEGSEYLAHVREGLAILQKFPTSAAAPGKRKLRLAVTPTF
                     SRSILIPRLRQFTDAYPEIDLTLQVSIPLLDVVAEDADLMVRFGAGRYADMEHVCLSK
                     DVVTPMASPAFVREHGPFESPMDLQNLPLLRSPLEPWRTWFAANGLDWPEPAEGSQFN
                     DIGLMCDGAAAGMGVGLIRLKLGAPWLENGSLVRLYENNVPSPHAHYLCWRTGAMDRW
                     EVAAFADWLKKAVG"
     gene            2568..>2754
                     /locus_tag="PKX82_10780"
     CDS             2568..>2754
                     /locus_tag="PKX82_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOF52784.1"
                     /translation="MNAPATPTASELLQKSELLAHILRGLKPYLPQHALLYQVEDTTP
                     YECDGLTAYRARPLLVAL"
BASE COUNT          542 a          900 c          829 g          483 t
ORIGIN      
        1 gggcacgttc actgagcatc accatggcgc aggcaggcga accgctccaa cccttttgcg
       61 gcgcgctcac cagcacgtcc acgccggtgt tttccatgtt gacccacatc gcaccagacg
      121 caatgcagtc gagcacaaac aatccaccca cttcgtgcgt ggcgtcagcc accttgcgca
      181 ggtagtcgtc gggcaggatc atgccggcag cggtctccac atgcggagcg aacaccacgt
      241 cgggtttttt ggcgcggatg gcggcgacca cttcgtccac tgggcaggga atccacggcg
      301 cttgcgcgct gtcgcccaca cggcgggcct tgagcacggt cgactcagca gggatgcttc
      361 ccatatcaaa gatctgcgtc cagcggtagc tgaaccagcc attgcgaatg accagggctt
      421 tcttgccggt cgcgaactgg cgcgccacgg cttccatgcc aaaagtacca ctgccaggca
      481 ccagcacagc cgacggggcg tggtacacct ctttcaggat gcgggagatg tccttcatca
      541 cgccctgaaa gcgctgcgac atgtggttca gcgcacggtc ggtgtacacc accgaaaact
      601 caagcaaccc gtccgggtca acgttggaca acaggcctgg cataaacagc tccggtaaaa
      661 atcaatgacg atgcgcgcag actagcacgc ccgcggcatc agcgcgacag gtgctccccc
      721 atggcctcac ccatggcgat gggtttggcc ggcgcccatt gcggattgaa ctccagtgtg
      781 gacggcggga acagcatcac taccgtagag cccagcagga aacgtcccat ctcctcgcct
      841 ttgcgcaaga ccaccttacc cgcgtcgtaa tgccattcgg tgacctgcgg cagacgtggc
      901 gggttcacca gaccatgcca gaccgtggcc atgctgccta cgatggtagc gcctaccagc
      961 gtcagcacaa acgggccgtg cgcagagtcg aacacgcaga ccacgcgctc gttgcgggca
     1021 aacaggccag gcacaccgcg tgccgtggtt gggttcaccg agaacaggtc gcccggaaca
     1081 tacaccatgc ggcgcagcac gccatcgcag ggcatgtgaa tgcggtggta atcccgcggg
     1141 ctgaggtaca gcgtggcaaa gctgcccccg ttgaaagtgg cacccagggc agcgtcccca
     1201 ccgaccagcg cagtagtgct gtagctgtga cccttggcct gaaaaatctg gtctttgtcg
     1261 atcgcgccaa actggctgat cgccccgtcc accgggctga tgaagtccgc cgccgccagc
     1321 gggcgtacac ctgcgcgcag gggacgggta aagaaatcgt tgaacgtcgg gtagctggca
     1381 atgtccggat tggccgcctc ggccatgttg acgttgtagc gtttcacgaa gcgccggatc
     1441 agcgccgtgg tggccgcgcc tccgtgggct ccggccagct ttcccgccag tgcggtcaac
     1501 agttttttgg ggagcaggta ctgcggcaaa accgcgaggg tgtcagacat ggcagctccc
     1561 aaaatgggcc tggtaatggg ggaacaagag gacgggcaag ggccgcgaat tatagtgggg
     1621 gcatgaccgc cgcacgcatc cctcccatcc agtgtctgtt gacgtttgag gcgctggcac
     1681 gcctgcgcag cgtcacacaa gcctccgagg aactgtgcgt cacgcccagc gccgtgagcc
     1741 accgcgtcaa acagctggag cagatcattg ggaccaagct gtttggtcgg gccgattttt
     1801 cactcaccac cgaaggcagc gaatacctcg cccatgtgcg cgaagggctg gccatcctgc
     1861 agaaattccc cacatccgct gcggcaccgg gcaaacgcaa actgcgcctg gcagtcactc
     1921 ccacgttctc gcgctcgatc ctgatcccgc ggttgcgcca gttcaccgac gcctaccccg
     1981 agatcgacct gaccttgcag gtctccatcc ccttgctgga cgtggtggcc gaagacgccg
     2041 acctgatggt gcgctttggt gccggtcgct atgccgatat ggagcatgtc tgtctgagca
     2101 aggacgtcgt cacaccgatg gcttcccccg cctttgtgcg cgaacatggc cccttcgaat
     2161 ccccaatgga cctgcagaac ctgcccctgc tgcgcagccc gctggagccc tggcgcacct
     2221 ggtttgctgc caacggactg gattggcctg aacccgcaga aggctcgcag ttcaacgaca
     2281 ttggcctgat gtgcgacggc gccgccgctg gcatgggcgt gggcctgatc cggttgaagc
     2341 tgggcgcgcc ctggctggag aacgggtcgc tggtgcggct gtatgagaac aacgtgccca
     2401 gcccccacgc ccactacctg tgctggcgca cgggtgccat ggaccgctgg gaagtggccg
     2461 cattcgccga ctggttgaag aaagcggtgg gttgacgcga agttttttca caccgacggc
     2521 ccgccaattg ccgtagagtt gcacccaccc ttgccaaagc cacgaccatg aacgcacccg
     2581 caacgcccac cgccagtgaa ttgctacaaa aatcagagct gctagcgcat attctgcggg
     2641 ggctaaagcc ctatttgcca caacatgcgc tgctgtacca ggttgaagac accacaccct
     2701 acgagtgcga tggcctgacg gcctaccgcg cccgtccgct gctggtggcc ttgc
//
LOCUS       DAOSJM010000331         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_31993_length_2749_cov_4.107275, whole genome shotgun sequence.
ACCESSION   DAOSJM010000331 DAOSJM010000000
VERSION     DAOSJM010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31993_length_2749_cov_4.107275"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1549
                     /locus_tag="PKX82_10785"
     CDS             2..1549
                     /locus_tag="PKX82_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF52785.1"
                     /translation="MQDPQTVRALGLSVWTGLLSTALATLLAAWVLSVTEGRSATQTH
                     RLTRWLAPMLAVPHAAFAIGLVALLAPSGWIVRGLSPWATGLDAPPPWTTTQDPWGLG
                     LVAVLVCKEVPFLLWAALAHVQRPDVARRLQQELRLAHTLGYSAHAAWWRVGWPQLLP
                     RLTAPLLAVLAYGLTVVDVALVIGPTTPPTLAVLAWQWLQDADPAMNAQGAAAAWLLA
                     GVLVLCAALAWSASRAPLWRLRWTRGADASPKQHTRHSSQTWFNTLNLLASVYLAVLL
                     ALLVGSVTGHWPFPQWLPDTWTWAAWHSVLGSGSTVWTTVGLALASAAVALVWAVAWL
                     EWAPPRWQLRSQPLLTLPLVLPAVLWVVGLHRLALDWQLDTTAWGLWLAHTLACLPYV
                     LLALQGPYSGFDARLRQVAATLGHGQWTFLLLVKWPLLRAALAASFAIGCAVSVAQYL
                     PTLYVGAGRFNTVTTEAVTLAAGGQRSLTAAFAWLQWLLPVLVFALAAHLGRAQFTHP
                     AHHDSRR"
     gene            1551..1976
                     /locus_tag="PKX82_10790"
     CDS             1551..1976
                     /locus_tag="PKX82_10790"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOF52786.1"
                     /translation="MTTPTEPRDIAPRIWSALPHAPAPGTPLGQRDALPDGIPQMLQL
                     DTGGGAEQPFRYLLLRSGEVVTAFVNRCAHFGVPLAARQEHLKFEPHVSISCNVHYAR
                     YRWADGVCDRGDCEGESLLPIPVTVDTSGHIRIALADTP"
     gene            1973..2608
                     /locus_tag="PKX82_10795"
     CDS             1973..2608
                     /locus_tag="PKX82_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52787.1"
                     /translation="MSLHIHITRLATPDTTLVSNLGITIAPGTVHTLMGDSGSGKSSV
                     LAAICGTLDGALAFEGEVLLDGARIDTLPTQARRVGLLFQEDLLFAHMTVRENLLFAV
                     PKGPQAGREAQVQQALADVEMVDSLHANPATLSGGQRARVALARALLAQPLALLLDEP
                     FSRLDAALRARMRALVFGMVRARNIPALLVTHDAADVADPALCTHLQQRRQ"
BASE COUNT          376 a          943 c          942 g          488 t
ORIGIN      
        1 gctgcaggac ccacaaaccg tgcgcgcact gggcctgagt gtctggactg gcctgctgtc
       61 caccgcactg gccacgctgc tggcggcttg ggtgctgtcc gtcaccgagg gacgctcggc
      121 aacgcaaacc caccggctga cgcgctggct ggccccgatg ctggcggtgc cgcatgccgc
      181 gtttgccatc gggctggtgg cactgctggc gcccagcggc tggatcgtgc gcggtctgtc
      241 gccctgggcc accggactgg atgcgccacc gccgtggacc accacgcaag atccttgggg
      301 cctgggactg gtggcggtgc tggtgtgcaa agaagtgcct tttttgctgt gggccgcgct
      361 ggcccacgtg caacggcccg atgtggcgcg ccgcctgcag caggaattgc gcctggccca
      421 caccctgggt tacagcgccc acgccgcctg gtggcgcgtg ggctggccgc aactcttgcc
      481 gcgcctgacc gcgccgctgc tggcggtgct ggcctacggc ctgacggtgg tggatgtggc
      541 gctggtcatc ggtcccacca caccgcccac attggccgtg ctggcctggc agtggctgca
      601 ggacgccgac ccggccatga acgcgcaggg cgcagcggcc gcgtggctgc tcgcgggcgt
      661 gctggtgctg tgcgctgcgc tggcttggtc cgcatcccgc gcgccactgt ggcggctgcg
      721 ctggacgcgc ggtgccgacg ccagccccaa gcagcacaca cggcacagct cccaaacatg
      781 gttcaacacc ttaaacctgc tggcaagcgt gtacctcgcg gtgctgctgg cgctgctggt
      841 gggcagcgtg acgggccact ggccgtttcc gcagtggctg cccgacacct ggacctgggc
      901 cgcctggcat tcggtgctgg gcagtggcag cacggtgtgg accaccgtgg gactggcgct
      961 ggccagtgcg gccgtggcac tggtgtgggc ggtggcctgg ctggagtggg cgccaccgcg
     1021 ctggcaactg cgtagccagc ccctgctgac cctgccgctg gtgttgcccg ccgtgctctg
     1081 ggtggtgggg ctgcaccggc tggcgctgga ctggcagctc gacaccaccg cctggggcct
     1141 gtggctggca cacacactgg cctgcctgcc ctatgtgctg ctggcactcc agggaccgta
     1201 cagcggtttc gatgcgcggc tgcgccaggt ggcggccaca ttgggccacg ggcagtggac
     1261 gtttctgctg ctggtgaagt ggccgctgct gcgcgcagcg ctggcggcca gctttgccat
     1321 cggctgtgcg gtgagcgtgg cgcaatacct gcccacgctg tatgtgggcg cgggacgctt
     1381 caacaccgtc accaccgaag cagtcacgct ggctgccggc ggacaacgct cgctcaccgc
     1441 ggcatttgcc tggctgcagt ggctactgcc ggtgctggtg tttgcactcg ccgcgcacct
     1501 gggccgggca caattcaccc accctgcaca ccacgactca cggcgctgac atgaccaccc
     1561 ccaccgaacc ccgtgacatc gcgccccgca tttggtccgc cctgccccat gcccccgcgc
     1621 cgggcacacc gctgggccag cgcgatgcat tgccggacgg catcccgcag atgctgcagc
     1681 tcgacaccgg cggtggcgcc gaacaaccgt ttcgctacct gctgctgcgc agtggcgaag
     1741 ttgtcactgc gtttgtgaac cgctgtgcgc actttggcgt gccgctggca gcgcggcaag
     1801 agcacctgaa gtttgagccg catgtgagca tcagctgcaa tgtgcactac gcacgctacc
     1861 gctgggcaga tggcgtctgc gaccggggcg attgcgaggg tgaatcgctg ctgccgattc
     1921 ccgtcaccgt ggataccagc gggcacatcc gcattgcgct ggcggacaca ccatgagcct
     1981 gcatatccat atcacccggc tggccacgcc cgacaccaca ctggtgagca acctgggcat
     2041 cacgattgcc ccgggcacgg tgcacacgct gatgggcgat agcggctcgg gcaagtccag
     2101 cgtgctggcg gccatctgtg gcacgctgga cggtgcgctg gcctttgaag gcgaagtact
     2161 gctcgacggt gcccgcatcg acaccctgcc cacgcaggcg cgccgcgtgg gcctgctgtt
     2221 tcaggaggac ctgctgttcg cccacatgac agtgcgcgag aacctgctgt tcgcggtgcc
     2281 caaagggccc caggccgggc gcgaagcgca ggtacaacag gctctggccg atgtggagat
     2341 ggtggactct ctgcatgcca acccggccac gctctcgggc ggccagcgcg cccgcgtggc
     2401 actggcgcgt gccctgctgg cccagccact ggcgctgctg ctggatgaac cgttttccag
     2461 gctggatgcc gcgctgcgcg cacgcatgcg cgcgctggtg tttggcatgg tccgggcgcg
     2521 gaacatccct gcgctgctgg tgacgcacga tgctgctgac gtggccgacc ctgcgctgtg
     2581 cacgcatttg cagcagcgca gacaatagcc ccatgctcga ccgctttgcg attgccctgc
     2641 tccgtccgcc catctccacg ctggcgcggg ttctggtacg cgtaggtatg ggcgccaaca
     2701 ctgtcacgct ggtgggtttt tccatcggca tgtttgctgc atttttgat
//
LOCUS       DAOSJM010000332         2743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_32081_length_2743_cov_3.546875, whole genome shotgun sequence.
ACCESSION   DAOSJM010000332 DAOSJM010000000
VERSION     DAOSJM010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32081_length_2743_cov_3.546875"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PKX82_10800"
     CDS             <1..536
                     /locus_tag="PKX82_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF52788.1"
                     /translation="LVGGASLLHALDGSGIWRGDVLFMAASLVWSTYSVLARRHALDA
                     VRATIAITAFAFVTYVPVYTVLLLLDWVPGQVLVAPWRDVVFQMVFQGMGSVVISGIT
                     FTKMIQHFGPVRSTMITALVPGLSAISAAIFLGEPLHWNLLAGLALVTLGIFFGVRAA
                     VAPKIIATSAISTGAKG"
     gene            551..1297
                     /gene="tsaB"
                     /locus_tag="PKX82_10805"
     CDS             551..1297
                     /gene="tsaB"
                     /locus_tag="PKX82_10805"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOF52789.1"
                     /translation="MNLLAFDTSTEVMSIGVSRVVDGVPRQWLHTSAGGAQASASLIA
                     TVMDLLQQAGLALVQLDAICFGAGPGAFTGLRTACAVAQGLAFGADVPVLPVDTLLAL
                     AEEARHAALPEVPACRVTALLDARMDEMYAATYDFADGQWSEVQGSHLVRPEDLVVAR
                     GAQAVQVLAGNVFTVYADRLASAEQNATTRITALPTAAALLRLAPGLLAAGKAVTAAE
                     ALPLYIRDKVAKTTAERMAEKAAAHAAAAQ"
     gene            1303..1770
                     /gene="rimI"
                     /locus_tag="PKX82_10810"
     CDS             1303..1770
                     /gene="rimI"
                     /locus_tag="PKX82_10810"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOF52790.1"
                     /translation="MNARIETTEAMFEPLLADRLDEVLRVEERAYAHPWNRANFLDAL
                     HSGYEAQMLMADGNVLGYFVAMKGVDEVHLLNITVAPEYQRQGWARVMLEALSIWARG
                     QNAVWLWLEVRVGNVRAMQVYESHGYRRVGYRKEYYPAAHGQREDAVVMSLRL"
     gene            1782..2579
                     /locus_tag="PKX82_10815"
     CDS             1782..2579
                     /locus_tag="PKX82_10815"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOF52791.1"
                     /translation="MALELDNRQRAMLEEMGVRVWLPGTAFATEAEPVPVRVVVAPAT
                     CVVPVPSAVTAPTAMPIAPMVRSVDASGLGWDALLQTVSSCQACPLGAGRKNVTLQPP
                     VQSQMDWMVVGDPPDEDEDRAGQPFAEAQGVLLDNMLKAVGASRSGQGAGGAYVTNVV
                     KCRPPHGRIAQPADLAQCAGYLQREMALVQPKMILAMGRFAVQLLLGEHGAMAGQPLG
                     KLRGTVYRYQGIPVVVTYAPKVLLRASADKGKAWADLCLAMDTLEVS"
     gene            2583..>2743
                     /locus_tag="PKX82_10820"
     CDS             2583..>2743
                     /locus_tag="PKX82_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOF52792.1"
                     /translation="MSPPLAPVEPPMALADEPGHLIRRAQQLAVSTFHEVHGRHVTPI
                     QYAILRTLH"
BASE COUNT          424 a          835 c          968 g          516 t
ORIGIN      
        1 tgttggtggg cggcgccagc ctgctgcatg cgctggacgg cagcggcatc tggcgtggcg
       61 atgtgctgtt catggcggca tcgttggtct ggtcgaccta cagcgtgctg gcgcgccgcc
      121 acgcgctcga tgccgtgcgc gccacgattg cgatcactgc gtttgcgttc gtcacctatg
      181 tgccggtcta caccgtgctg ttgctgctgg attgggtgcc ggggcaggtg ctggtggctc
      241 cctggcgtga cgtggtgttc cagatggtgt tccagggcat gggctcggtg gtgatctccg
      301 gcattacctt caccaagatg attcagcact tcggcccggt gcgctcgacc atgatcacgg
      361 cgctggtgcc ggggctgtcg gccatttcgg ccgccatctt tctgggcgag ccgctgcact
      421 ggaatctgct ggccgggctg gcgctggtga cgctgggtat cttctttggc gtgcgcgccg
      481 cagttgctcc taaaataata gctacctccg caatttccac gggggctaag ggctgatttg
      541 gcttgaaacc atgaacttgt tggcgtttga taccagtacc gaagtcatgt ccatcggcgt
      601 ctcgcgcgtg gttgatggcg tgccgcgcca gtggctgcat accagcgccg gcggcgcgca
      661 ggcgtctgcc agcctgattg ccaccgtgat ggacttgctg cagcaggccg ggctggcctt
      721 ggtgcaactc gacgcgatct gttttggggc tggccccggc gcctttaccg gcttgcgtac
      781 tgcgtgcgcg gtagcgcagg ggctggcgtt tggtgccgac gtgccggtgc tgccggtgga
      841 taccctgctg gctttggccg aagaagcgcg tcatgcggcc ttgcccgagg tgcctgcgtg
      901 ccgggtcacc gcgctgctgg atgcgcgcat ggacgagatg tatgcggcca cttacgactt
      961 tgcggacggc caatggagcg aagtgcaggg cagccatctg gtgcgccccg aggacctggt
     1021 ggtggcgcgt ggcgcgcagg cggtgcaggt gctggcgggc aacgtgttca cggtgtacgc
     1081 cgaccgcctg gccagtgctg aacagaatgc aactacgcgg atcacggctt tgccgaccgc
     1141 ggcggccctg ctgcggctcg cacccggact gctggcggcc ggcaaggcgg tcacggccgc
     1201 cgaggcgctg cccctctaca tccgtgacaa agtcgccaaa accacggccg agcgcatggc
     1261 ggagaaggcg gcggcccatg cagccgcggc acaatagggc gcatgaacgc acgcattgaa
     1321 acgacggagg cgatgtttga gcccctgctg gctgaccggc tggacgaagt cctgcgggtg
     1381 gaagaacgtg cctatgccca cccctggaac cgggccaatt ttcttgacgc actgcattcg
     1441 ggctatgagg cgcaaatgct gatggcggac ggcaatgtac tcggctactt cgtggccatg
     1501 aagggggtgg atgaggtgca tctgctcaac atcacggtcg cgcccgaata ccagcgccag
     1561 ggctgggccc gcgtcatgct ggaagcgctg tcgatctggg cgcgtggcca gaacgcagtg
     1621 tggctgtggc tggaagtgcg cgtgggcaat gtgcgcgcca tgcaggtcta tgagagccat
     1681 ggctatcgtc gcgtgggcta ccgcaaggaa tactacccgg cagcccacgg ccagcgcgaa
     1741 gacgcggtgg tcatgagcct gcggctgtaa ggacgcaccg gatggcactg gaactcgaca
     1801 accgccaacg cgccatgctt gaagagatgg gtgtgcgcgt ctggctgccc ggtaccgcgt
     1861 ttgccaccga ggcagaaccg gtgccggtgc gggtggtggt tgcgccggca acgtgcgttg
     1921 tgcctgtacc ttccgcagtg accgcaccca ctgccatgcc gattgcccca atggtgcgca
     1981 gcgtggatgc ctcgggcctg ggctgggatg cgctgctgca aaccgtctcc agctgccagg
     2041 cctgcccgct gggtgccggg cgcaagaatg tgacgcttca acctccggtc cagagccaga
     2101 tggactggat ggtggtgggc gatccgcccg acgaagacga ggaccgtgcc ggccagccct
     2161 ttgccgaggc gcaaggcgtg ctgctggaca acatgctcaa ggcggtgggc gccagccgtt
     2221 cgggccaggg tgctggcggc gcttatgtga ccaacgttgt caaatgccgc ccgccacacg
     2281 gccgcattgc gcagcccgcc gatctggcgc agtgcgcggg ctatctgcag cgcgaaatgg
     2341 cgctggtgca acccaaaatg attctggcca tgggccgctt tgcggtgcaa ctgctgctgg
     2401 gcgaacatgg ggcgatggcg gggcagccac tgggcaagct gcgtggcacg gtgtaccggt
     2461 accagggcat cccggtggtg gtgacgtatg cacccaaggt gctgctgcgc gccagtgccg
     2521 acaagggcaa ggcctgggcc gatctgtgtc tggcgatgga cacgctggag gtgtcctgag
     2581 acatgagtcc accgcttgct cctgttgagc cgcccatggc gctggcggac gagcccggcc
     2641 acctgatccg ccgtgcgcag cagctggccg tgtccacctt ccatgaggtg catggccgcc
     2701 atgtcacccc gatccagtac gccatccttc gcaccttgca cga
//
LOCUS       DAOSJM010000333         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_32208_length_2735_cov_3.983209, whole genome shotgun sequence.
ACCESSION   DAOSJM010000333 DAOSJM010000000
VERSION     DAOSJM010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32208_length_2735_cov_3.983209"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /gene="arsJ"
                     /locus_tag="PKX82_10825"
     CDS             complement(<1..1034)
                     /gene="arsJ"
                     /locus_tag="PKX82_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HOF52793.1"
                     /translation="MNQGARNYAIVTSAYWGFTLTDGALRMLVLLHFYKLGYSPFTLA
                     FLFLLYEAAGIAANLIGGWLATRYGIARMLAVGLTTQITGFLLLSALSPDWTATMSVA
                     WVVLSQGVCGVAKDLTKTASKSAIKLTAGEASGQLFKWVAWFTGSKNAMKGIGFFLGG
                     VLLEVLGFRASLWAMAGLLSLVLLGVVVYVPKLMGKSKASKSAKELFAKNQGINLLAA
                     ARVVLFGARDVWFVVGVPVFLYASGWSRPMVGGFLALWTIGYGAVQAFAPHLVKRSPD
                     GLSTEVPAARLWSALLAVVPVALAVVVYLDVPNLEWVVVGGLGVFGFAFAVNSSVHSY
                     LVLAYAGSEKA"
     gene            complement(1036..2079)
                     /locus_tag="PKX82_10830"
     CDS             complement(1036..2079)
                     /locus_tag="PKX82_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HOF52794.1"
                     /translation="MRVGINGMGRIGRLALRAAMGAAERQSDDPRAGNRLEVVHLNEL
                     KGGAKATAHLLAFDTVQGKWRENIRAEGEEAILIGAKRLSFSSHATAAEIPWGDMGVD
                     VVLECTGKFLTPETIQGHLDRGAKRVIVAAPVKVGDVLNIVVGVNHQLYEPAKHRIVT
                     AASCTTNCLAPVVKVIHEHLGIKHGQITTIHNPTNTNLVVDAPHKDLRRARSAMESLA
                     PTSTGSATAIALIYPELKGKLNGHAVRAPVLNASLTDCVFELQRATTPEGVNALFKTA
                     SETYLAGILGYEERPLVSADYARDTRSSIVDALSTMVTDGTLLKVYAWYDNEMGYACR
                     MVDLACHMEAVGI"
     gene            complement(2158..>2735)
                     /locus_tag="PKX82_10835"
     CDS             complement(2158..>2735)
                     /locus_tag="PKX82_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52795.1"
                     /translation="SHSHPPKQSRLVHIQVHVDVIDQLFNSIDPAPFHVRELDADAHA
                     YIVHSAKALPRDARMELVLHIDSLPADESKLSTVSQAIQTHFSGKAVAERIELQDLFA
                     RGRISLLIGLSILSLSIGLVEISKSWAADNHWLQILQQSLLIGGWVAMWRPMEIFLYD
                     WWPMAAHIRLLRRLATIKVHIAPQPKPSASA"
BASE COUNT          569 a          817 c          811 g          538 t
ORIGIN      
        1 gccttctccg atccagcgta cgccagcacc aggtaggaat gcaccgagga gttgacagcg
       61 aaggcaaagc cgaaaacgcc cagaccaccc acaaccaccc attccaggtt gggcacgtcc
      121 aggtagacca ccaccgccag cgcaacgggg accaccgcga gaagtgccga ccacagccgt
      181 gcggcgggta cttcggtgct cagaccatca ggactgcgtt tgacaaggtg cggcgcgaag
      241 gcctgcaccg ccccatagcc aatcgtccat agcgccagaa aacctccgac catgggacgg
      301 gaccagccgg atgcgtacag aaacaccgga acacccacca caaaccacac atcccgtgcg
      361 ccaaacagca ccacgcgggc agctgccagc aggttgatgc cctggttttt ggcaaacagc
      421 tctttggcgg acttggacgc tttgcttttg cccatgagct tgggaacgta cacaacgacg
      481 cccagcaaca ccaggctcag caaacccgcc atggcccaga gcgacgctct gaatcccagc
      541 acctccagca gtacgccacc cagaaaaaag ccaatacctt tcatggcgtt cttgctgccg
      601 gtaaaccagg ccacccattt gaacaattga ccggatgcct cgcccgcagt cagcttgatg
      661 gccgatttgc tggcggtttt ggtgaggtct ttggccacgc cgcacacccc ctgtgacagc
      721 accacccagg ccaccgacat cgtggctgtc cagtccggcg acaacgcgga taacagaagg
      781 aagccggtga tctgggtcgt cagacccaca gccagcatgc gcgcaatgcc gtagcgcgtg
      841 gccagccagc caccaatcag attggcggca atgcccgcag cttcgtacag caagaacaga
      901 aacgccagcg taaacgggga gtagcccagc ttgtagaagt gcaacagcac cagcatgcgc
      961 agcgcgccgt cggtgagcgt gaagccccag tacgcgctgg tgacgatggc gtaattgcgt
     1021 gcgccctggt tcatatcaga tgcctacggc ttccatgtgg caggccagat ccaccatgcg
     1081 gcaggcgtaa cccatctcgt tgtcgtacca ggcgtagacc ttcagcagcg tgccgtcggt
     1141 caccatggtg gagagcgcat cgacaatgga gctgcgggtg tcgcgggcgt aatcggcgct
     1201 caccagcggg cgctcctcgt agcccagaat gccggccaga taggtttcgg atgcggtctt
     1261 gaacagagca ttcacccctt ccggtgtggt tgcacgctgg agttcgaata cacagtcggt
     1321 cagcgatgca ttgagcacgg gggcgcgcac cgcatggccg ttgagcttgc ctttgagttc
     1381 ggggtaaatc agcgcaatcg cagtcgcact gcctgttgac gtgggtgcca gactttccat
     1441 ggcactgcgt gcgcggcgca aatctttgtg gggcgcatcg accaccagat tcgtgttggt
     1501 cgggttgtgg atggtggtga tttggccgtg cttgatgcca aggtgctcgt gaatgacctt
     1561 gaccacgggt gccagacagt tggtggtgca ggaagctgcg gtaacgatgc ggtgcttggc
     1621 aggttcgtaa agctggtgat tcacccccac cacgatgttg agaacatcgc ccactttgac
     1681 cggcgcagca acaatgaccc gcttggcacc ccggtccagg tggccctgaa tggtttctgg
     1741 cgtcaggaat ttgcctgtgc actccagcac cacatccacc cccatgtcgc cccaagggat
     1801 ttcggcggcg gtggcatggg agctgaagga gagtcgtttg gccccgatca ggatcgcttc
     1861 ttcgccttcg gcacggatgt tttcgcgcca cttgccttgc accgtatcaa atgccagcaa
     1921 gtgtgccgtt gctttggctc cgcctttgag ttcgttcaag tgcaccacct ccagccggtt
     1981 gcccgcacgg gggtcgtctg attgccgctc ggccgcgccc atggcggccc gcagtgccag
     2041 gcggccgata cggcccattc cgttgatacc tacacgcatc gggagctcct ttttattcaa
     2101 tgattcaata attattgaag tataggggcg ctgtatgacc gatttgtgac tttggaatca
     2161 cgctgaggca gaaggcttcg gctggggggc gatgtgcact ttgatggtgg ccagacgcct
     2221 gagtagtcgg atatgggcag ccatgggcca ccagtcatag agaaagattt ccatgggccg
     2281 ccacatggcg acccagccgc caatgagcag actctgttgc aggatctgga gccaatggtt
     2341 gtcggcagcc cagcttttgc tgatttctac caatccaatc gaaagcgaca ggatcgacag
     2401 cccaatcagc aggctgatac gtccgcgtgc gaacaagtct tgcagctcga tgcgctccgc
     2461 cactgccttg cccgagaagt gcgtctgtat ggcctggctc acggtgctca gcttgctttc
     2521 gtccgcgggc aggctgtcga tgtgcaatac caattccatg cgcgcatctc ttggcaatgc
     2581 cttggcgcta tggacgatat aggcgtgtgc gtctgcgtcc aactcgcgta catgaaacgg
     2641 tgcggggtca atggaattga acaattggtc aatgacgtcc acgtggacct ggatgtgtac
     2701 aaggcgggac tgctttggag ggtggctatg tgaag
//
LOCUS       DAOSJM010000334         2717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_32501_length_2717_cov_3.533058, whole genome shotgun sequence.
ACCESSION   DAOSJM010000334 DAOSJM010000000
VERSION     DAOSJM010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32501_length_2717_cov_3.533058"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..1373
                     /locus_tag="PKX82_10840"
     CDS             234..1373
                     /locus_tag="PKX82_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HOF52796.1"
                     /translation="MRVSQLTLHTTEPAGADFAALAATQPQLVLAFGAVDALKGAAGA
                     LAAAFPQAHRIGCSTAGEISASGVSDGTLVVTALALAKTRVEQVSTELKTMEDSQPAG
                     RRLASGLSRDGLRAILVFGQGVHINGSAMLAGMIEVVGKDIPITGGLAGDGGAFAQTW
                     VLDDQGASSDRLACVGLYGEGLTFSHGSFGGWSPFGPARKVTRCENNVLFELDGEPAL
                     AVYRRYLGEHAKDLPASGLLFPFAMLGSDHSERGLIRTILGIDEAAGSLTLAGDIEPN
                     GYLKLMHANTDALVDGAEAAAQAARDMHQAAGQSLALLVSCVGRKLVMGGRVDEEVEA
                     VGEVFGQGATLAGFYSYGEISPFVRATECKLHNQTMTVTCIAETP"
     gene            1396..>2717
                     /locus_tag="PKX82_10845"
     CDS             1396..>2717
                     /locus_tag="PKX82_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF52797.1"
                     /translation="MHKLLSRQIKRTLGVDDAQLSAVLEEIAHVGSRGGLSAQATTVI
                     AGLGGLLQRVDEAYVQNDRDLELKTRSLELSSVELTESNSRLRAELGSRTRAMESLRA
                     TAMGLMEFVDLDQPGLLDDNLENLSTLMSALVRQKEESQRDLQSALTDLAHQKFALDQ
                     HAIVSTTDVNGNIIYANDKLCEISGYSRAELLGKNHRLINSGFHDRAFFIGMWQTITA
                     GNVWHGEICNRNKAGGLYWVNATIVPLRDEAGKPSMFIAIRTDITERKKMESTIKAAE
                     ARLLRITNAVPGVVFQAHVSPTHFKYTFVSDRVEDVLGLQARALIDDGFLTFRQIIEE
                     DREKVIQGVQDAAQRRAAWREEYRARLPNGLLRWIRTEVNPEPDLAPDGATVFTGIWQ
                     DVTELKEADARLREVTQNVPVAVFQYYIAGNGKFRIPFMSDAIEKMCGV"
BASE COUNT          542 a          749 c          886 g          540 t
ORIGIN      
        1 cattgcccaa ctgaaggcta cgctggccaa tttcaagatt cccaagcgct gctttgtcgt
       61 tcctgaattg ccgcgaaacg ccatgggcaa ggtgcagaag aacctgttgc gttcccagta
      121 ccaggcggtg taaccggccc aatccctttc gggggcaact gcaggcatgg aaagcctggt
      181 ttcaggcgta ttatcctttt gactgtatac aggtagcgaa caggagtatt gccatgcgcg
      241 tgtcccaact gaccctgcac acgacagaac cggctggtgc agatttcgcg gcgctcgctg
      301 ccacgcaacc gcagttggtg ttggcatttg gcgccgtgga cgcgctcaag ggagctgctg
      361 gtgccttagc cgcagccttt ccacaggcac accgtatcgg ctgttctacc gccggtgaaa
      421 tttccgcatc cggggtgagt gacggcacgc tggtggtcac ggcgctggca ctggccaaga
      481 cccgcgtgga acaggtcagt accgaactga agacgatgga ggactcgcag cctgcaggca
      541 ggcgcctggc atccggtctg tcgcgggatg gcttgcgggc gatcctggta tttgggcaag
      601 gcgttcacat caacggaagc gccatgctgg ctggcatgat cgaagtggtg ggtaaggaca
      661 ttcccatcac cggcggtctc gctggcgatg gtggtgcctt cgcccagacc tgggtgctgg
      721 atgatcaggg cgccagcagc gaccgccttg cgtgtgtcgg tttgtatggc gaggggctga
      781 ctttctcgca cgggtcgttt ggcggctggt cgccttttgg cccggcgcgc aaggtcacac
      841 gctgtgaaaa caatgtgctg tttgaactcg atggtgagcc cgcgctggcg gtctaccgac
      901 gctatctggg cgagcatgcc aaggacttgc ctgcgtccgg actcctgttt ccgtttgcca
      961 tgctcggcag tgaccacagt gagcgggggt tgattcgcac cattctcggt atcgatgagg
     1021 cggcgggcag cctcacgctg gcgggggata tcgaacccaa tggctacctg aaactcatgc
     1081 atgccaatac cgacgcactg gtggacggag cagaagcggc cgcgcaggcg gccagggaca
     1141 tgcaccaggc cgcgggccaa agcctggccc tgctggtcag ctgcgtgggg cgcaagctgg
     1201 tcatgggcgg gcgtgtggat gaagaagtgg aggcggtggg tgaggtcttt ggccagggcg
     1261 cgactttggc cggcttctat tcctacggcg agatcagtcc ttttgtgcgg gccacggaat
     1321 gcaagctgca caaccagacg atgacggtca cctgcatcgc agaaacaccc tgaactccag
     1381 cggcccacga atttcatgca caaacttctc tcgcgccaga tcaaacgcac actgggggtg
     1441 gatgacgcgc agctgtccgc cgtgctggag gagattgcac acgtggggtc caggggcggg
     1501 ctgtctgccc aagccacgac ggtgatcgcc gggctgggcg gtttattgca gcgggtcgat
     1561 gaggcgtatg tccagaacga ccgggacctg gagctcaaga cacgcagtct ggaactgagt
     1621 tcggtggagc tgacggagag caactcgcgt ttgcgcgcgg aactggggag ccgcacccgg
     1681 gccatggaat ccttgcgtgc cacagcgatg ggactgatgg aatttgtgga tctggaccaa
     1741 cccggcctgc tcgacgacaa cctggagaat ctctccacgc tgatgagcgc gctggtgcgt
     1801 cagaaagagg aaagccagag agacctgcag tcggccctga ccgacctggc gcaccagaag
     1861 tttgcgctgg accagcatgc gattgtcagt accacggatg tcaacggaaa catcatctat
     1921 gccaacgaca agctgtgcga gatcagtggg tattcgcgcg cagagttgct gggcaagaac
     1981 caccgtctga tcaattcggg tttccacgac cgggcatttt tcatcggaat gtggcaaacc
     2041 attaccgctg gcaatgtctg gcatggagag atttgtaatc gcaacaaggc cggaggcctg
     2101 tattgggtca atgcgaccat tgttcccttg cgggatgagg cgggcaagcc gtcgatgttt
     2161 attgccatcc gaaccgacat caccgagcgc aagaagatgg agtccaccat caaggcggcc
     2221 gaggcccggt tgctgcgcat caccaatgcc gtgccggggg tggttttcca ggcacatgta
     2281 tcgccaacgc atttcaagta cacgtttgtc agcgacaggg tagaagacgt attgggattg
     2341 caggcccggg ccctgattga cgatggattt ctcactttcc gccagatcat tgaagaagac
     2401 cgcgagaagg tcattcaggg tgtgcaggat gctgctcagc gccgtgcggc gtggcgcgaa
     2461 gaataccggg cgcgtctgcc caatggcctg ttgcggtgga tccgtaccga ggtcaatccg
     2521 gagcctgatc ttgcgcccga tggcgccacc gtgtttaccg gcatctggca ggacgtgacc
     2581 gaactcaagg aggcggatgc ccgtctgcgg gaagtcacgc agaacgtgcc tgtggcggtt
     2641 ttccaatact acatagcggg caacgggaag ttccggattc cctttatgag tgacgccatc
     2701 gaaaagatgt gcggtgt
//
LOCUS       DAOSJM010000335         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_32769_length_2698_cov_3.945516, whole genome shotgun sequence.
ACCESSION   DAOSJM010000335 DAOSJM010000000
VERSION     DAOSJM010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32769_length_2698_cov_3.945516"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /gene="hisB"
                     /locus_tag="PKX82_10850"
     CDS             <1..582
                     /gene="hisB"
                     /locus_tag="PKX82_10850"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOF52798.1"
                     /translation="SAEVTRNTAETRITVRVNLDGTGQSRLHTGIGFFDHMLDQIARH
                     GMIDLDIEADGDLHIDGHHTVEDVGITLGQAFHQAVGDKKGIRRYGHAYVPLDEALSR
                     VVIDFSGRPGLVMDVPFKAGMIGSLDTQLVHEFFQGFVNHAFVTLHIDNLKGENAHHQ
                     AETVFKAFARALRFAVELDPRALGTIPSTKGSL"
     gene            633..1286
                     /gene="hisH"
                     /locus_tag="PKX82_10855"
     CDS             633..1286
                     /gene="hisH"
                     /locus_tag="PKX82_10855"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOF52799.1"
                     /translation="MKKVAVVDYGMGNLRSVTQAVMHVAADAGVEVVWARTPQEVMDA
                     ERVVLPGQGAMRDCMRELHDSGMFDAVMHAAANKPLMGVCVGMQMLLDHSQELDTACL
                     GLIPGDVVKFDLAGQHQPDGSRYKVPQMGWNQVWQSGQHGRHPVWGDVPDGAYFYFVH
                     SYYAQPSDERHTVGETDYGQRFTSAIARDNIFATQFHPEKSAEHGLALYRNFLHWNP"
     gene            1355..2095
                     /gene="hisA"
                     /locus_tag="PKX82_10860"
     CDS             1355..2095
                     /gene="hisA"
                     /locus_tag="PKX82_10860"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOF52800.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMDQSTTFGEEPDVMARSWVDKGA
                     RRLHLVDLNGAFAGHPKNEMAIRKILKEVGSEVDVQLGGGIRDLDTIERYLDAGLRYV
                     IIGTAAVKNPGFLQDACTAFGGHIIVGLDARDGKVATDGWSKLTRHDVIDLGKKFEDY
                     GVESIIYTDIGRDGMLSGINIDATVKLAQALSIPVIASGGLSNMADIEALCEVESEGV
                     EGVICGRAIYSGDLDFQKAQERADELNG"
     gene            2186..>2698
                     /locus_tag="PKX82_10865"
     CDS             2186..>2698
                     /locus_tag="PKX82_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOF52801.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNDQGA
                     DELTFLDITATSDGRDLILHIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKTSF
                     NSAAIANPQVIEDASHKYGAQCIVVAIDAKRRFGEDMLNRGEGWDVYSHGGRKNTGLD
                     AVAWATQMAEH"
BASE COUNT          543 a          827 c          827 g          501 t
ORIGIN      
        1 agtgccgaag tcacccgcaa caccgccgag acccggatca ccgtccgcgt caacctcgac
       61 ggaaccgggc agtcccggct gcataccggc atcggcttct ttgaccacat gctcgaccag
      121 atcgcccgcc acggcatgat cgacctcgac atcgaggccg acggcgattt gcatatcgac
      181 ggccaccaca cggtggaaga cgtgggcatc accctgggcc aggcgttcca tcaggccgtc
      241 ggcgacaaaa aggggattcg ccgctacggc cacgcctatg tgccactgga cgaggccttg
      301 agccgcgtgg tgattgattt ttcgggccgc cccgggctgg tgatggacgt gccgttcaag
      361 gccggcatga tcggtagcct ggatacccaa ctggtccacg agttctttca gggttttgtg
      421 aaccacgcgt ttgtgacatt gcacatcgac aacctcaagg gcgaaaacgc ccaccaccag
      481 gccgagaccg tgttcaaggc ctttgcccgt gcactgcgct ttgccgtgga gctggatccg
      541 cgcgcgctgg ggaccatccc ctccaccaag ggctcgctct agtttatgta tcaaattggc
      601 ctctaccctc cgtcgaatgg gcgtgggcag ctatgaaaaa agtagcagtt gtagattacg
      661 gcatgggcaa cctgcgatcg gtcacccagg ccgtgatgca tgtggcggcc gatgcgggtg
      721 tcgaagtcgt gtgggcgcga accccgcaag aggtgatgga cgccgaacgt gtggtgttgc
      781 ctgggcaggg cgccatgcgc gactgcatgc gcgagttgca tgactcgggc atgttcgacg
      841 ccgtcatgca tgcggctgcc aacaagccct tgatgggtgt gtgtgtgggc atgcagatgc
      901 tactggacca cagccaggaa ctcgacaccg cctgcctggg gctgatcccc ggtgatgtgg
      961 tcaagttcga tctggccggc cagcaccagc ccgatggtag ccggtacaag gtgccgcaga
     1021 tgggctggaa ccaggtgtgg caatcgggcc agcatggtcg ccaccccgtt tggggcgatg
     1081 tacccgatgg tgcgtacttt tactttgtgc acagttacta cgcccagccg tcggatgagc
     1141 gccacaccgt aggggaaacc gactatggtc agcgttttac ctcggccatt gcgcgtgata
     1201 acattttcgc aacccagttt caccccgaaa aaagcgcaga acacggcttg gccctctacc
     1261 ggaacttcct ccactggaat ccctgacctc catctgccgc gcttattact ggttccgcat
     1321 ccctttccta ttcttgttcc accactgaag caccatgctt ttaattcctg cgattgacct
     1381 caaagacggc cactgcgtac gcctcaaaca aggcgacatg gaccaatcca ccacgttcgg
     1441 cgaggagccc gacgtcatgg cccgcagctg ggtggacaag ggcgcgcggc gtctgcatct
     1501 ggtggacttg aacggcgcgt ttgccggaca tcccaagaac gagatggcca ttcgcaagat
     1561 cctcaaggaa gtcggcagtg aagtggacgt gcagctcggc ggcggtattc gcgacctcga
     1621 cacgattgag cgctacctcg acgccggcct gcgctacgtc atcatcggta ccgccgcggt
     1681 caagaatccc ggcttcctgc aggacgcctg caccgcgttt ggcggccaca tcatcgtggg
     1741 gctggacgcc cgtgacggca aggtggccac cgacggctgg agcaagctca cccgccacga
     1801 cgtgatcgac ctgggcaaaa aatttgaaga ctacggtgtt gaaagcatca tctacaccga
     1861 catcggccgt gatggcatgc tcagcggcat caacatcgac gccaccgtca agctcgcgca
     1921 agcgctctcc attcccgtca tcgcctcggg cggcctttcc aacatggccg acattgaggc
     1981 cctgtgcgaa gtggaaagcg aaggcgtcga aggtgtgatc tgcggacgcg ccatctacag
     2041 cggcgacctg gacttccaga aagcgcaaga gcgcgcggat gagttgaacg gatgagcatg
     2101 ttttgccgaa gggccgcccc aaggcaaaac gcggccccct tggggggcag ggagctacac
     2161 gtagtgagcg accgtggggg caagcatgct cgctaaacgc atcattccct gtctggacgt
     2221 gaccggtggc cgcgtggtca agggcgtcaa tttcgtcgag ctgcgcgatg ccggggaccc
     2281 ggtggaaatt gcggcgcgtt acaacgacca gggtgcggac gaactgacgt ttctggacat
     2341 cactgccacc agcgatggcc gcgacctgat tttgcacatc atcgaagcgg tggcctccca
     2401 ggtgttcatt ccgctcaccg tgggtggcgg agtgcgtacc gtagaagatg tgcgccgcct
     2461 gctcaatgca ggggccgaca agaccagctt caattcggcg gccattgcca atccgcaggt
     2521 catcgaagac gcatcccaca aatacggtgc acagtgcatc gtggtcgcca ttgatgccaa
     2581 gcgccgcttt ggcgaggaca tgctcaaccg cggtgaaggc tgggatgtct acagccacgg
     2641 tgggcgcaag aacaccggcc tggacgccgt ggcctgggcc acgcagatgg cagagcac
//
LOCUS       DAOSJM010000336         2692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_32877_length_2692_cov_3.801289, whole genome shotgun sequence.
ACCESSION   DAOSJM010000336 DAOSJM010000000
VERSION     DAOSJM010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32877_length_2692_cov_3.801289"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PKX82_10870"
     CDS             <1..331
                     /locus_tag="PKX82_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HOF52802.1"
                     /translation="AINASLWQRMPEDATTDDNPEISDLVGRGEIAGAWNVNPDNTLV
                     MTVRHSLQANANGSIRLGWLRKVGTSSDRPGLRFHTEIFSGYGDTLLDYNRKRTVLSM
                     GLTLLDW"
     gene            complement(343..900)
                     /locus_tag="PKX82_10875"
     CDS             complement(343..900)
                     /locus_tag="PKX82_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52803.1"
                     /translation="MFNRLFGKGISKSTSSSMRDAVDSRGLEVTEDDPDTAWSRWEEA
                     QAAQDAWAFVHSPISAPLPLIHDLLPPSAIDDVPTQPLGLEDLTLEQRKTRALVIIQE
                     HHPRVANTIRTLWGYRECSIYINKLVMDGDDGRGHARVGFNQDAVQAMLALIDVHDAV
                     YGTFDAKMPTGFADPSIRAGWDGSR"
     gene            complement(1034..1561)
                     /locus_tag="PKX82_10880"
     CDS             complement(1034..1561)
                     /locus_tag="PKX82_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOF52804.1"
                     /translation="MQPSRYHIALRILHWLTAVMLLVALVMGTLVLDKTPNTSPEKMD
                     ALRGHMVFGGLILVLTLIRLVVRLRTEHPAPVVTGMAFADRLAPLVHWGLYALVIVMA
                     ASGIGISILAGLPDIVFGGQGALPTDFKNFPPRLVHGIVAKLLMLAIALHVGAALFHQ
                     LVRKDRLLSRMGFGK"
     gene            complement(1764..>2692)
                     /locus_tag="PKX82_10885"
     CDS             complement(1764..>2692)
                     /locus_tag="PKX82_10885"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOF52805.1"
                     /translation="RLTLSADAPKRMSLAGAQHKLLVVYHEGHLYEPEGATPSTHILK
                     PNHPDTDSYPASVVNEYLTMRLAKAVGLPVPQVHMLYVPEAVYVIQRFDRFTTEATPP
                     GARPPLNVQRMHLIDACQLLNKSRTFKHSGASIQALKDVIEATTNKANTRLMLFRWLV
                     FNILVANDDCHLKNLSFFMSPEGVQLAPHYDLLATSAYYTKAFANEKATWADVRMAIP
                     LGGANFFSDVTVQLVLDAGLELGVPEVSARRILKEVTTRLPEALGKELDGIRKLHAGV
                     PEAAKVQLAAEDRLLRVFEHIILKEMLERLRS"
BASE COUNT          583 a          794 c          801 g          514 t
ORIGIN      
        1 tgccatcaac gccagcctgt ggcagcgcat gccggaagac gccaccacgg atgacaaccc
       61 ggaaatctct gacctggtgg gacgcggcga gatcgctggt gcctggaacg tgaacccgga
      121 caacacgctg gtgatgacgg tgcgccattc gctgcaggcc aacgccaacg ggtcgatccg
      181 gctgggctgg ctgcgcaagg tgggtacgtc cagtgatcgc cccggcttgc ggtttcacac
      241 cgagattttc agtggctacg gcgatacttt gctggactac aaccgcaaac gcaccgtcct
      301 gagcatgggt ctgacgttgc tggactggta ggagccgagg tcctagcggg acccgtccca
      361 cccggcccgg atggacgggt cggcaaaacc cgtaggcatt ttggcgtcaa aagtgccata
      421 cacggcatcg tgcacatcga tcagcgccag catggcctgc actgcgtcct ggttgaaccc
      481 gacccgggca tgaccccggc catcatcgcc gtccatgacc agcttgttga tatagatgct
      541 gcattcccgg tagccccaca gggtacggat ggtgttggcc acacgggggt ggtgctcctg
      601 aatgatgacc agcgcccggg ttttgcgctg ttccagcgtc aggtcttcca gaccgagcgg
      661 ctgcgtgggc acatcgtcga tggccgacgg cggcaacagg tcatggatga ggggcagggg
      721 tgcactgatc gggctgtgca cgaacgccca tgcatcctgg gcagcttgcg cctcttccca
      781 acggctccag gcggtgtcgg ggtcgtcttc ggtgacttcc aggccacgcg aatccaccgc
      841 atcccgcatc gacgacgaag tggacttcga aatgcctttg ccaaaaagcc tgttgaacat
      901 accctacccg aaaaatgtaa cgagatgtta cttcggccgc attgcggaat ccagccctgc
      961 cccggcagga aacccgcgta attgtgactt tatgcggctt ccgggaaaaa tgaaagtcgc
     1021 cggggcaatc cgtttatttc ccaaacccca tgcgggaaag caaacggtcc ttgcgcacca
     1081 gttggtggaa cagcgcggcc ccaacatgca acgcaatggc cagcatcaat agcttggcaa
     1141 caatgccgtg gaccaaccgc gggggaaagt tcttgaaatc ggtcggcaat gcaccctgtc
     1201 caccaaacac aatgtccggc aggccggcga ggatggagat tccaatgccg ctggcagcca
     1261 tcacgatcac cagggcatac agcccccagt gcaccagggg agccagacgg tctgcgaagg
     1321 ccatgccggt taccacggga gcaggatgct ccgtgcgcag acgcacgaca aggcgaatca
     1381 gcgtcagcac cagaatcagt ccgccaaaca ccatatgccc ccgcaaggca tccatcttct
     1441 ccgggctggt attgggcgtc ttgtccagca ccagcgtccc catcaccaag gcaacgagca
     1501 gcatgacagc ggtcaaccag tgaagaatac gcagggcaat gtggtaacgg gaaggttgca
     1561 tggaatctct cctgataacg tgatgtaccc attggatcac aacggcgcta cgcgtcagaa
     1621 agcaaaaagc ccatcgcagt ggatgggctt gtacgcatgg agttgtccac aggaccgccc
     1681 cagggtctag ccatcacttg catcgcgcta caagtttgca tacctgaagg acgtcggcaa
     1741 ggcatggatt ctccctgtgc atttcagctg cgcaaacgct caagcatctc tttgaggatg
     1801 atgtgctcga atacccgcaa cagccggtct tctgctgcca gttgaacttt tgcagcttcc
     1861 ggaaccccgg cgtggagctt tcgaatcccg tccagctcct tgcccaacgc ctcaggcaga
     1921 cgggtggtta cttctttgag aatacggcgt gcgctcacct caggaacgcc cagctccagc
     1981 ccggcatcca gcaccagctg gacagtgaca tcgctgaaga agttggcccc gcccaaagga
     2041 atggccattc gtacatcggc ccaggtggcc ttttcgttgg caaacgcctt ggtgtaatac
     2101 gcactggtcg ccagcaaatc gtaatgcggg gccaactgca cgccttcagg ggacatgaag
     2161 aacgacaggt tcttcaagtg acagtcgtcg ttggctacaa ggatgttgaa caccaaccac
     2221 cgaaacaaca tgaggcgcgt gttggctttg ttggtggtgg cctcgatgac atctttgagc
     2281 gcctgaatcg acgcgcccga atgcttgaag gtgcgcgact tgttgagcaa ctggcaggca
     2341 tcgataaggt gcatgcgttg cacattcaat ggcggccggg cgccaggtgg cgttgcctct
     2401 gtggtgaacc ggtcgaaccg ttgaatgaca tagaccgcct ccggcacata gagcatgtga
     2461 acttgcggaa ccgggagacc gaccgccttt gccaatcgca tggtgaggta ttcgttgacc
     2521 acggacgcgg ggtacgagtc ggtatcaggg tgattgggct tgaggatgtg ggtggaaggc
     2581 gttgcaccct ctggctcgta gagatgtcct tcgtggtaca ccaccaaaag cttgtgttgc
     2641 gcccccgcca gggacatgcg cttgggcgca tcggcgctca gtgtcaacct gg
//
LOCUS       DAOSJM010000337         2689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_32916_length_2689_cov_5.911162, whole genome shotgun sequence.
ACCESSION   DAOSJM010000337 DAOSJM010000000
VERSION     DAOSJM010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32916_length_2689_cov_5.911162"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="PKX82_10890"
     CDS             <1..660
                     /locus_tag="PKX82_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52806.1"
                     /translation="ANDLVHAGHTVHVADPSARPLAALLPAAAGEQLAQALGALGVQW
                     HFGATVQAVDTADTRLTVTLSDGSVVVADAVLSAIGLRADTRLAAAAGIACERGIVVD
                     ARLQSSAPGVYALGDCAQYASAGQRTLPYVMPIMHAAKALAGTPTEVVFPLMPVSIKT
                     PALPIVVAAAHPAQQGEWVADAQEDGVWRFIDTDGVQRGFVLTGRHTTRRMELAKTTL
                     G"
     gene            795..>2689
                     /locus_tag="PKX82_10895"
     CDS             795..>2689
                     /locus_tag="PKX82_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOF52807.1"
                     /translation="MQDLPSFTPVSNSVEKKAQLRQAALEYHEFPTPGKIAVAATKQL
                     INQHDLALAYSPGVAAPCEEIVKDPNNAFKYTSRGNLVAVITNGTAVLGLGDIGPLAA
                     KPVMEGKGVLFKKFAGIDVFDIEINEKHDLDKLVEIIASLEPTFGGINLEDIKAPDCF
                     YVERKLRERMKIPVFHDDQHGTAIVVGAAILNGLKVVGKDPKKIKLVTSGAGAAALAC
                     LGLLVKLGIPRENIYVTDLAGVVYEGRTELMDEDKIQFAQKTDARTLREVIQGADVFL
                     GLSAGGVLKADMVTTMAAKPLIFALANPTPEILPEEVKAVRDDAIMATGRTDYPNQVN
                     NVLCFPYIFRGALDAGASTITLEMEIAAVHAIAELAQAEQSEVVAAAYVGEQLAFGPE
                     YLIPKPFDPRLMMKIAPAVAKAAADSGVALRPIADMDAYRERLQSFVYASGTMMKPIF
                     NAAKNALKKRVAYAEGEDERVLRAAQIVVDEHLAFPTLIGRPKVIAERIEKFGLRLQE
                     GKHYNVVNVEQDDRYRDFWQTYHQMTARKGVTIPMAKIEMRRRLSLIGAMMLHKGYVD
                     GLICGTWGNTALHLQYIDQVIGKRPGGSPSTPQDVHIYAAMNGLMLPGRQVFLVDTHV
                     NYDPTAE"
BASE COUNT          543 a          842 c          841 g          463 t
ORIGIN      
        1 gccaatgacc tggtgcacgc cgggcacacg gtgcatgtgg ccgacccgtc tgcacggccc
       61 ctggccgcat tgctgcctgc cgcagcgggt gaacagctgg cgcaagcgct gggcgcactg
      121 ggtgtgcaat ggcactttgg ggccacggtg caggcggtcg ataccgcaga cacccgattg
      181 accgtcaccc tgtcggatgg cagcgtggtg gtcgccgatg cggtgctcag cgccattggc
      241 ctgcgcgccg acacgcgtct agccgcggca gcgggcatcg cctgcgaacg cggcatcgtc
      301 gtggacgccc ggttgcagtc ttcggcaccc ggcgtgtatg ccctcggaga ctgtgcgcaa
      361 tacgccagcg caggccagcg cactctgccc tatgtcatgc ccatcatgca tgccgccaaa
      421 gcgctggccg gtacgcccac cgaggtggtc tttccgctga tgccggtgtc gatcaagacg
      481 ccggcactgc ctatcgtggt ggcggccgcg catccggccc agcaaggcga atgggtggcc
      541 gatgcgcagg aagacggcgt ttggcgcttt atcgacaccg atggcgtgca acgcggcttt
      601 gttctgacag gtaggcacac gacccggcgc atggagctgg cgaagaccac attgggctga
      661 ataaggtcat gactgatctg catgaaactg tggctttcca caaacccggc ccaaggggtg
      721 aggtctacaa tccaccaccc aatcaagagt agggcggatg caccgttgca ctggccctcg
      781 cctgaggatg caccatgcaa gatctgccca gcttcacccc cgtctccaac tctgtggaaa
      841 aaaaggcgca attgcgccag gccgccctgg agtaccacga gttccccacc cccggaaaaa
      901 ttgcggtcgc tgccaccaag cagctgatca accagcacga cctggcgctg gcctactcgc
      961 ccggcgtggc tgcaccgtgc gaggagatcg tcaaggaccc gaacaacgcc ttcaagtaca
     1021 ccagccgcgg taacctggtt gcggtgatca ccaacggcac cgcggtgctg ggcctgggcg
     1081 acattggccc gctggccgcc aagccggtga tggaaggcaa aggcgtgctg ttcaagaagt
     1141 ttgccggcat cgatgtgttt gacatagaaa tcaacgaaaa gcatgacctg gacaagctgg
     1201 tcgagatcat tgcgtcgctg gaaccgacct ttggtggcat caacctcgaa gacatcaagg
     1261 cgcctgattg cttctatgtg gaacgcaagc tgcgcgagcg catgaagatt ccggtgttcc
     1321 acgacgacca gcatggcacg gccattgtgg tgggtgcagc gattctgaat ggcctgaagg
     1381 tagtgggcaa agaccccaag aaaatcaagc tggtgacctc tggcgcgggt gccgctgcgc
     1441 tggcctgcct gggcctgctg gtcaagctgg gcattccccg cgaaaacatc tatgtgacgg
     1501 atctggccgg cgtggtgtac gagggccgca ccgagctgat ggacgaagac aagatccagt
     1561 tcgcccagaa gaccgatgcg cgcacgctgc gggaagtgat ccagggcgca gacgtgttcc
     1621 tgggtctgtc ggctggcggc gtgctcaagg ccgacatggt gaccaccatg gccgccaaac
     1681 cactgatctt tgcgctggcc aatcccacgc cggaaatcct gccggaagaa gtcaaggcgg
     1741 tgcgcgacga cgccatcatg gccaccggcc gcacggatta ccccaaccag gtcaacaacg
     1801 tcctgtgctt cccctacatc ttccgcggcg ccctcgacgc gggcgcctcc accatcacgc
     1861 tggagatgga gattgccgca gtgcatgcca ttgccgaact ggcccaggcc gagcagagcg
     1921 aagtggtggc agcggcctac gtgggggagc aactggcctt tggccccgag tacctgattc
     1981 ccaagccgtt tgatccgcgc ctgatgatga agattgcccc ggccgtggcc aaggccgcag
     2041 cggacagcgg cgtagccttg cgcccgattg ccgacatgga cgcctaccgc gagcggctgc
     2101 aaagttttgt gtatgcgtct ggcaccatga tgaagccgat cttcaatgcg gccaagaacg
     2161 cactcaagaa acgcgtggcc tacgccgagg gtgaagacga gcgcgtactg cgtgcggccc
     2221 agatcgtggt ggacgaacac ctggctttcc ccacgttgat tggtcgcccc aaggtgattg
     2281 ccgagcgcat cgagaagttc ggcctgcgcc tgcaggaagg caagcactac aacgtagtca
     2341 acgtggagca ggacgaccgc taccgcgact tctggcagac ctaccaccag atgacggcgc
     2401 gcaagggcgt aaccatcccc atggccaaaa ttgagatgcg ccggcgcctc tcgttgatcg
     2461 gcgccatgat gctgcacaag ggttacgtgg acggtctgat ctgcggcacc tggggcaaca
     2521 ctgccctgca cctgcagtac atcgaccagg tgattggcaa acggcccggc ggcagcccca
     2581 gcacaccgca ggatgtacac atttacgccg cgatgaacgg cctgatgctt cccggtcgcc
     2641 aagtgtttct ggtggatacc catgttaact acgatcccac tgccgaaga
//
LOCUS       DAOSJM010000338         2685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_32993_length_2685_cov_4.322814, whole genome shotgun sequence.
ACCESSION   DAOSJM010000338 DAOSJM010000000
VERSION     DAOSJM010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32993_length_2685_cov_4.322814"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PKX82_10900"
     CDS             <1..297
                     /locus_tag="PKX82_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOF52808.1"
                     /translation="ELANLALHCTRQEDAARKVERQLRKSEAALLLETRIGQIFSGVI
                     TGSAAEGVWVRIFSPPAEGKLYAEAPGLAIGDKVQVRLESVSVERGFIDFVVVD"
     gene            complement(314..1171)
                     /locus_tag="PKX82_10905"
     CDS             complement(314..1171)
                     /locus_tag="PKX82_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOF52809.1"
                     /translation="MQATLYALGAIALWVTLASLGVALRHVPPFLLTGVALLVGSTLA
                     LPFVAKDRQQWKIPARTLLLGVYGLFGYHFLLFIALRLAPPVEANLVNYLWPLLMVVL
                     SPLLLRNVHLRAMHVAAALMGFAGAAVAILGANASPAGSLAVSGTWSWGYLPALGSAL
                     IWASYSLWTQRVPAFPTAAVGLFGLVSGVLSLLCHWALEPQVVLSGQDWLLLVLMGLG
                     PLGAAFFLWDKALKLGDARHIGILSYITPLASTSLLMLVSGRPFSWSIVLSASMIISA
                     ALLGARARR"
     gene            complement(1171..2220)
                     /gene="mgrA"
                     /locus_tag="PKX82_10910"
     CDS             complement(1171..2220)
                     /gene="mgrA"
                     /locus_tag="PKX82_10910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HOF52810.1"
                     /translation="MTYQAKPERYNGSMPYRTCGTSGLKLPAISLGLWHNFGDATPFQ
                     TQRDMLRTAFDLGITHFDLANNYGPPYGSAETNFGEHLRRDFKPYRDELIISSKAGWD
                     MWPGPYGQGGGSRKYLLASLDQSLQRMGLDYVDIFYSHRFDPDTPLEETMGALASAVQ
                     QGKALYVGISSYSAGKTREAAAILRSMGVRALIHQPSYSLLNRWIEEDLLDALADTGM
                     GCIAFSALAQGLLTDKYLHGIPADARINRPGGGSFTPDHLSAQNLQHVSALNAIAQSR
                     GQTLAQMAIAWVLRDARVTSALIGASKPAQIVELAAAMQKPNFSTSELAAIDQHAVEG
                     GINLWQRPSTDQRPS"
     assembly_gap    2288..2297
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2322..>2685
                     /locus_tag="PKX82_10915"
     CDS             2322..>2685
                     /locus_tag="PKX82_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52811.1"
                     /translation="MLPVTEESPKIQEPRIWSADGWTAKVIKNEDDDGWAVAMTRDGA
                     REPALIGPWTMGRDKKNPKPLDNNAFNTLVKTASEFVRRSEQQLHATLHQSITVTAGG
                     ARVTVYLGIEPDDDNPSAT"
BASE COUNT          532 a          820 c          878 g          445 t
ORIGIN      
        1 gaactggcaa acctggcttt gcattgcacc cgccaggaag atgcggcccg caaagtggag
       61 cgacagctgc gcaaatcgga agcggcgctg ttgctggaga cgcgtatcgg gcagatcttc
      121 agcggcgtca tcaccggcag tgccgccgaa ggcgtctggg tgcggatctt ttcgcccccg
      181 gcagagggca agctctacgc ggaagcaccc ggcttggcta tcggcgacaa agtgcaggtg
      241 cggctggagt cggtcagcgt ggagcgcggg tttatcgact tcgtcgtggt tgactaacgc
      301 gagagtgatg cactcacctg cgcgcccgcg cgcccagcaa ggctgcggaa atgatcatgg
      361 aggctgacag gacaatgctc cagctgaacg gccgccccga caccagcatc agcaggctgg
      421 tcgatgccag gggggtgatg taactcagaa tgccaatgtg ccgggcatcg cccagcttga
      481 gcgccttgtc ccacaaaaag aacgctgcgc ccagtggccc cagccccatc agcaccagca
      541 gcaaccagtc ctgacccgag agcaccacct gcggttccag cgcccagtgg catagcaggg
      601 acagcacgcc agacaccagc ccgaagagcc ccaccgcagc ggtgggaaac gccggtacgc
      661 gttgggtcca cagcgaatag ctggcccaga tcagcgcaga ccccagtgcg ggcaaatagc
      721 cccaggacca cgtaccagaa acagccagac tccctgcggg cgatgcattg gcacccagaa
      781 tggcaaccgc cgccccggca aaccccatca gggcagccgc gacatgcatt gcgcgcaaat
      841 gcacgttgcg cagcaacagc ggggagagca ccaccatcag caggggccac aggtagttca
      901 ccaggttggc ctccacagga ggggccaacc gcagcgcaat gaacaacagg aagtggtagc
      961 caaacaatcc gtacacgccc agcagcaggg tgcgcgcagg aattttccat tgctgccggt
     1021 ctttggccac aaagggcaat gccagcgtgc tgccaacgag cagcgccacg ccagtgagca
     1081 ggaacggggg cacgtggcgc aacgccacgc ccagcgaggc cagcgtaacc cagagcgcaa
     1141 tggcgcccag tgcatagagt gtggcttgca tcagctgggg cgctggtcgg tggacgggcg
     1201 ctgccagagg ttgatgccgc cttcgacggc atgctggtca atcgcggcca gctcgctggt
     1261 gctgaagttg ggcttctgca tggcagctgc aagctcgacg atttgcgcgg gcttgcttgc
     1321 accgatcagt gccgaggtca cgcgtgcatc gcgcagcacc caggcaatgg ccatctgcgc
     1381 cagggtttgg ccgcgcgact gggcaatggc gttgagcgcg ctcacatgct ggaggttctg
     1441 cgcgctcagg tggtcgggcg tgaacgagcc gccgccgggg cggttgatac gggcatccgc
     1501 cggaatgcca tggaggtact tgtcggtcag caggccttgc gccagcgcgc tgaaagcgat
     1561 gcagcccatg ccggtgtcag ccagtgcatc gagcaaatct tcctcaatcc agcggttgag
     1621 caggctgtag gacggctggt ggatcagggc gcgtacaccc atgctcctca gaatggctgc
     1681 ggcctcacgc gtcttgccag ccgagtagct gctgatgccc acgtacagcg ccttgccctg
     1741 ctgcaccgcg ctggccagtg cacccatggt ttcttccagc ggggtgtcgg ggtcaaagcg
     1801 gtggctgtag aagatgtcga catagtccag gcccatgcgt tggaggctct ggtccaggct
     1861 ggccagcagg tatttgcgcg agccgccgcc ctggccatag gggccgggcc acatgtccca
     1921 gccggctttg ctggagatga tgagttcgtc acggtagggt ttgaaatcgc ggcgcaggtg
     1981 ttcgccaaag ttggtttcgg cgctgccgta gggcgggccg tagttgttgg ccaggtcgaa
     2041 gtgggtgatc cctaagtcaa acgccgtgcg cagcatgtcg cgttgggtct ggaacggggt
     2101 ggcatcgcca aagttgtgcc acaggcccag ggaaatcgcc ggcagcttga gcccgcttgt
     2161 gccgcaggtg cggtagggca tgctgccgtt gtagcgctcg ggtttggctt ggtaggtcat
     2221 gggggtagat actatcgaac tttcccaaag ctttgtggct cgcctgcctg tgactgaaga
     2281 acctaccnnn nnnnnnncga actctcccat agctttgtgg tttgctgcct gtgactgaag
     2341 aatctcccaa aatccaagaa ccccgcatct ggtccgctga cggctggacc gccaaagtga
     2401 tcaagaacga ggatgacgac ggctgggctg tggccatgac ccgggacggt gcgcgcgagc
     2461 cggccctgat cggcccgtgg accatgggtc gcgacaagaa gaatcccaag cccctagaca
     2521 acaacgcctt caacacgctg gtcaagaccg ccagtgagtt tgtccggcgc agcgagcagc
     2581 agctgcatgc caccctgcac cagagcatca cggtcaccgc aggcggtgcg cgggtgacgg
     2641 tgtacctggg catcgagccc gatgacgaca acccttctgc cacct
//
LOCUS       DAOSJM010000339         2673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_33193_length_2673_cov_4.319328, whole genome shotgun sequence.
ACCESSION   DAOSJM010000339 DAOSJM010000000
VERSION     DAOSJM010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33193_length_2673_cov_4.319328"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKX82_10920"
     CDS             complement(<1..299)
                     /locus_tag="PKX82_10920"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011659328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOF52812.1"
                     /translation="MQLPFSLGTTLPIIQAPMAGVQGSALAAAVCNAGGLGSLPCAML
                     SPQQLRDELTQLRQLTDMPFNVNFFCHTPPAPDAAREARWRSALAPYYADYGIDPS"
     gene            375..2503
                     /gene="uvrB"
                     /locus_tag="PKX82_10925"
                     /pseudo
     CDS             375..2503
                     /gene="uvrB"
                     /locus_tag="PKX82_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464467.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
     gene            2605..>2673
                     /locus_tag="PKX82_10930"
     CDS             2605..>2673
                     /locus_tag="PKX82_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOF52813.1"
                     /translation="MRLTTKGRFAVTAMIDLGLRQSS"
BASE COUNT          564 a          753 c          838 g          518 t
ORIGIN      
        1 gatgggtcga tgccgtagtc ggcgtaatag ggcgccaaag cactgcgcca gcgcgcctct
       61 cgggccgcat cgggcgcggg gggcgtgtgg cagaagaaat tgacgttgaa gggcatgtcg
      121 gtcagctggc gcagttgcgt cagttcgtcc cgtaactgct gggggctcag catggcgcag
      181 ggcagggaac ccaggccccc ggcgttgcag acggccgctg ccagggcgct gccctgcacg
      241 ccggccatgg gggcttgaat gatgggcagc gtggtaccca gggaaaaagg aagttgcatc
      301 gtgtctggac ctgaaatgtc aacctagtct acagtcatgg gtttggcctt tcgcctgagc
      361 ccgagatcac caccatgtcc gtacccgcac ccctggctgt agccgtatcc gatgacgttg
      421 agacggttcg cacgggcacg tttgtgcgtt ttccgggctc accgtttgag ttgtaccagc
      481 cgtacccgcc tgccggggac cagcccgagg ccatcaacca gctggtcgaa ggcgtggaag
      541 acggtgaggt attccagacc ctgctgggcg tgactggctc tggaaagacc ttcaccatgg
      601 ccaacgtgat tgcgcggctg ggtcggccag ccattgtgtt ttgccacaac aagacgctgg
      661 cggcgcagct gtacagcgaa ttccgcgagt tcttccccaa gaatgcagtg gaatatttcg
      721 tcagctacta cgactactac cagccagagg cctatgtgcc gcagcgcgat ctgtttatcg
      781 agaaagacag cgccatcaac gagcacattg agcagatgcg gctctcgtgt accaagagca
      841 tcctggagcg gcgcgatgtg gttattgtgg ccaccgtctc cgccatctat ggtatcggcc
      901 agcccgaggc gtatcacaag atgatcatga cactgcgcgc gggcgacaaa ctgggccagc
      961 gtgatgccat tgcgcaactg gtgcgcatgc agtaccagcg caacgacatg gatttttccc
     1021 gcggcacctt tcgcgtgcgc ggcgacacca tcgacatctt ccccgctgaa cattccgaaa
     1081 tggcgcttcg gctggagctg ttcgacgacg agattgagag tctgcagctg tttgatccgc
     1141 tcaccggtcg cattcgccag aagattccgc gttttacggt ctaccccagc agccactatg
     1201 tcaccccgcg tgaacaggtg ttgcaggcgg tggaaaccat caagctggaa ctggccgatc
     1261 gtctgaaaga gttcgtcagc cagggcaagc tggtcgaagc ccagcgattg gagcagcgca
     1321 cccggtttga tctggaaatg ctcagcgaag tggggcactg caaaggtatt gaaaattact
     1381 cgcgccatct ggctggcagc gcgccgggtg atccgccatc cacgctggtc gactacttgc
     1441 cgcgcgattc gctgatgttt atcgacgaaa gccacgtcac catcggccag tttggagcca
     1501 tgtatctggg tgaccggtcc cgcaagacga cgctggtgga gtacggcttc cggctgccca
     1561 gcgcgctgga caaccggccg ctgaagtttg acgagtttga gcagcgcatg cgccaggtgg
     1621 tcatggtgtc ggccacacct gcccagtggg aaaaagacca ttcgagcaag gtggtggagc
     1681 aggtggtgcg ccccactggc ctgatcgacc cagaagtgga agtgcgtccc gccacccacc
     1741 aggtggacga cgtgctgcag gaaatccgta tccgggtcga gaagaacgaa cgggtactga
     1801 tcactacgct gaccaagcgc atggccgagc aactgaccga ctatctgacg gacaacggcg
     1861 tcaaggtgcg ttatttgcac agcgacgtgg acaccgtgga gcgcgtggag attttgcgcg
     1921 acctgcgcct gggtaccttt gatgtgctgg tgggtatcaa cctgctgcgc gaggggctgg
     1981 acattcccga ggtgtcgctg gttgcgattc tggatgcaga caaggaaggc ttcctgcgtt
     2041 ctgagcgttc actgatccag acgattggcc gggcggcgcg caatctggaa ggcaaagcca
     2101 ttctgtatgc cgacaaagtc accgactcca tggcgcgggc gatcagcgaa ccgagcgccg
     2161 tcgcaagaaa cagattgccc acaacctcga gcagggtatt acgcccaagg ccatcgtcaa
     2221 gaaggtgcgc gacctgatcg acggtgtcta cagcgagaag gcgggcaagg aagccgagcg
     2281 tctggaacgc gatgcccagc agcgtgcgca ggtggaggac atgagcgaga aagatatttc
     2341 ccgtgaaatc aagcgcctgg agaagctgat gatggagcac gcccgcaatc tcgaattcga
     2401 aaaggcggcg cgggtgcggg accaattgac cattttgaaa gagcaggcat tcggtgcctc
     2461 cggctcggac aaccttgcgg ttcttgcatc tccctccaaa tagtcgggta ttcgaccgat
     2521 cagtctaggt atccgagcga aatacttggt ttattgttat acttgactcc attagtcaac
     2581 caaatacgcc gggaggagca tgcgatgcgt ctcactacca aaggccgctt tgcggtcaca
     2641 gcgatgattg atctgggtct gcgccagagc agc
//
LOCUS       DAOSJM010000340         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_33505_length_2654_cov_4.026549, whole genome shotgun sequence.
ACCESSION   DAOSJM010000340 DAOSJM010000000
VERSION     DAOSJM010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33505_length_2654_cov_4.026549"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="PKX82_10935"
     CDS             <1..892
                     /locus_tag="PKX82_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOF52814.1"
                     /translation="RPHLSFLHRFAQTLKAQREVVRGKPENFNRPDYNFRLVGNDGQT
                     PQGTEQVQISVRQRGAPLDLIVAEAMILANSSWGQWLAELGVPAIYRSQASMAPGVKV
                     RMGTKALPHAGIGVKSYAWSTSPLRRYTDLVNQWQIIACARHGKTAALVVPFKPKDAE
                     LFAIISAFDTAYSAYNGYQGAMERFWTMRYVQQHGITELVASVFKENMVRADDIPLVL
                     PVMGAQGLPRNAKVRVKLGEMDLITLDVHGTVVERLDAPADTAESTDESEDEDDVAGP
                     IAIAVDVSENPEPAGDNPAP"
     gene            889..1761
                     /locus_tag="PKX82_10940"
     CDS             889..1761
                     /locus_tag="PKX82_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HOF52815.1"
                     /translation="MNLRFIPRFSTLQWALGVSIAVHVVLLTVRFVDPESFNRVFEDT
                     PLEVILVNARTEEKPDKVKAIAQASLAGGGDAEKGRATSPLPPSPSTDSGDALEQASS
                     ARQLQSLQEHQSLLLAQVKNQLATMPPPDPKEVASKAEQTEREEKRKQLTKLLAEIER
                     RINMENSRPKKRYISPATKEAVYAVYYDTLRHAIEDRGTENFPSAGGKKLYGELTMIV
                     TVNHDGRVLAAEVVQTSGNPTLDRRAMAIAKSAGPFGPFNKAMRQQADQILVVSRFRF
                     TRDETLEANVTGSP"
     gene            1758..2474
                     /gene="mtgA"
                     /locus_tag="PKX82_10945"
     CDS             1758..2474
                     /gene="mtgA"
                     /locus_tag="PKX82_10945"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOF52816.1"
                     /translation="MKAAWRWLGLMALALFALQLYFVIRVAAMVVIAPQSTAFERSEA
                     WRISREKSEFRWRQQWVDYPQISDHLKRAVIASEDDGFANHDGVDWDALEKAWAKNTQ
                     AEQRAQQRSNAKPPKIVGGSTITQQLAKNLFLSGERTMVRKGQEFVLTFLLERLLTKR
                     RILEIYLNSVEWGEGVFGAEAAAQHYFRKPASKLNAFEAARLAVMLPRPKYFEKLPNS
                     AYLSGRASVITGRMADAQLP"
     gene            2507..>2654
                     /locus_tag="PKX82_10950"
     CDS             2507..>2654
                     /locus_tag="PKX82_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOF52817.1"
                     /translation="MLAKLMSLFLLGLIRVLTGSQARWHGCPPKAEQRIYFANHQSHA
                     DLVMI"
BASE COUNT          552 a          865 c          810 g          427 t
ORIGIN      
        1 gcgcccacac ctaagctttt tgcaccgctt tgcgcagacg ctcaaggcgc agcgtgaggt
       61 ggtacgcggc aagccggaaa acttcaaccg acccgactac aacttccgcc tggttggcaa
      121 cgatggccag acgccgcagg gcaccgagca ggtgcagatc agcgtgcgcc agcgcggcgc
      181 accactggac ttgattgtgg ccgaggccat gattctggcc aacagcagtt ggggtcaatg
      241 gctggcggag ctgggcgtgc ccgccatcta tcgcagccag gccagcatgg cccctggggt
      301 gaaggtgcgc atgggcacca aggccttgcc gcatgccggc atcggcgtga agagctatgc
      361 ctggagcacc tcgccgctac gccgctacac cgacctggtg aaccagtggc aaatcattgc
      421 ctgcgcgcgc cacggcaaga ccgccgcgct ggtggtgcca ttcaaaccca aggacgccga
      481 gctgttcgcg atcatctcgg cctttgacac cgcctacagc gcctacaacg gctatcaggg
      541 cgccatggag cgtttctgga ccatgcgcta cgtgcagcag cacggcatca ccgagctggt
      601 ggccagcgtg ttcaaggaaa acatggtgcg cgccgacgac attccgctgg tgctgccggt
      661 catgggtgcc cagggcctgc cgcgcaatgc caaggtacgg gtcaagctcg gcgagatgga
      721 cctgatcacg ctggacgtgc acggcaccgt cgtggagcgg ctggacgccc ctgccgatac
      781 cgctgaaagc accgacgaaa gcgaggacga agacgacgtg gcgggaccca tcgccattgc
      841 cgtggacgtc agcgaaaacc ccgaaccagc cggcgataat ccagcaccgt gaacctgcgc
      901 ttcatccccc gcttcagcac actccagtgg gcactgggtg tctccatcgc cgtgcacgtg
      961 gtgctgctga cggtgcgctt tgtggatccg gagagcttca accgcgtgtt tgaggacacg
     1021 ccgctggagg tcatcctcgt caacgcacgc accgaagaga aacccgacaa ggtcaaggcg
     1081 attgcgcagg cctccctggc cggcggtgga gatgccgaaa aaggccgcgc caccagccca
     1141 ctgcctccat caccgtcgac ggactccggg gacgcgctgg agcaggccag cagtgcgcga
     1201 cagctgcaaa gtctgcagga gcaccagtcc ctgctgctgg cccaggtgaa gaaccagttg
     1261 gccaccatgc cgccgcccga cccgaaagag gtcgccagca aagccgaaca gaccgagcgc
     1321 gaagaaaagc gcaagcagct caccaagctg ctggccgaga tcgagcggcg tatcaacatg
     1381 gaaaactcgc ggcccaagaa gcgctacatc agccccgcga ccaaggaggc ggtgtacgcg
     1441 gtgtactacg acaccctgcg ccacgccatt gaggaccggg gcacggagaa cttcccctct
     1501 gcgggtggca agaaactcta cggtgaactc accatgatcg tcacggtcaa ccacgatggc
     1561 cgcgtgctcg ccgccgaagt ggtgcagacc tccggcaacc ccacgctgga ccgccgggcc
     1621 atggcgattg ccaaatccgc agggcctttt ggtccgttca acaaggccat gcgccagcag
     1681 gcagaccaga ttctggtggt gtcacgcttc aggttcaccc gcgacgaaac gctggaagcc
     1741 aacgtcactg gcagcccatg aaagcggcat ggcggtggtt ggggctgatg gcactggcgc
     1801 tgttcgcgtt gcagttgtat ttcgtcatcc gcgtggccgc catggtggtc atcgcgccgc
     1861 agtccaccgc gttcgagcgc tccgaggcct ggcgcatcag ccgtgagaaa agcgaatttc
     1921 gctggcgcca gcaatgggtg gactacccgc agatctccga ccacctcaag cgcgcggtga
     1981 ttgcttctga agacgacggc tttgccaacc acgatggcgt ggactgggac gcactggaaa
     2041 aagcctgggc caaaaacacc caggcggaac agcgcgccca gcagcgcagc aacgccaagc
     2101 cccccaagat cgtcggcggc tccaccatca cccagcaact cgccaaaaac ctgtttctct
     2161 caggggaacg caccatggtg cgcaaggggc aggagtttgt actgaccttt ctactggagc
     2221 gcttgttgac caaacggcgc attctggaga tttacctcaa cagcgtcgaa tggggcgaag
     2281 gcgttttcgg tgcagaagca gcggcacaac actacttccg caaaccagcc tccaaactca
     2341 atgcctttga ggccgcgcgc ctggctgtca tgctgccgcg cccgaagtac ttcgaaaaac
     2401 tgcccaattc ggcctacctc tcgggccgag ccagcgtgat cacgggccgc atggcggacg
     2461 cgcagttgcc ataaagctgg caccttcgct ctgtatcatg ggctccatgc ttgccaagtt
     2521 gatgagtctg ttcctgctgg gcctgatccg ggtgttgacc ggatcccaag cgcgctggca
     2581 tggctgcccg cccaaggccg agcagcgcat ctactttgcc aaccaccaga gccacgccga
     2641 tctggtgatg atct
//
LOCUS       DAOSJM010000341         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_33710_length_2641_cov_3.886698, whole genome shotgun sequence.
ACCESSION   DAOSJM010000341 DAOSJM010000000
VERSION     DAOSJM010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33710_length_2641_cov_3.886698"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="PKX82_10955"
     CDS             complement(<1..1299)
                     /locus_tag="PKX82_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOF52818.1"
                     /translation="MRTQLKITAGQHSDKGRKDANQDFHGLCVPRELQLAGKGIAIAL
                     ADGISSSSVGHVASESAVKGFLSDYYCTSDAWSVKRSAQRVLEATNSWLYAQTQQSQW
                     RYDRDRGYVCTFSALVLKSTTAHLFHIGDARICRLQGQALEQLTTDHRVFVTHDKSCL
                     SRALGVQQQLDLDYLALPLEVGDIFVLTTDGVHEHMDGAFVAGAIAQAGDDLDRAARA
                     ITEEALRRGSADNLTLQIVRVDELPAPEAAEISTLSVTLLPPPLPSDGTVIDGFRIVR
                     ELHASFRSHLYLAQDEATGQQLAIKIPSLDMRDDTAYLERFLMEEWVARRIDNTHVLR
                     PYAQTRKRTALYITLEYLEGQTLAQWMRDHPAPDLETVRGMVEQIARGLQAFHRLEML
                     HQDLRPENIMIDPQGTLKIIDLGATRVSAVQEALPVGQNED"
     gene            complement(1311..2117)
                     /locus_tag="PKX82_10960"
     CDS             complement(1311..2117)
                     /locus_tag="PKX82_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HOF52819.1"
                     /translation="MSYVIPSEFVTKMVDAGESKIFMSTRDTLIRSYMAGAILALAAA
                     FAVSVTVNTGNPLIGALLFPVGFCILYLLGFDLLTGVFTLAPLAVMDKRPGCTWSGVL
                     RNWSLVFVGNFAGALTVALFMAIIFTNGFVDAPNAIGQKIGHIGEGRTVGYAAYGAAG
                     MLTLFIRAVMCNWMVSTGVVAAMMSTSVSGKVIAMWMPILVFFYMGFEHSIVNMYLFP
                     SGLMLGGKFTFMDYLIWNEIPTVVGNLVGGLTFVGATLYSTHYKTAPKRR"
     gene            complement(2173..>2641)
                     /locus_tag="PKX82_10965"
     CDS             complement(2173..>2641)
                     /locus_tag="PKX82_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF52820.1"
                     /translation="QLTMLAALMGAVVRVAGGWISDHWGGVNTLTVVLVAVAVTLVGV
                     GLSAGSLVMTTLLLILCFAALGAGNGALFQLVPLRWPTSTAVAGSMIGEIGALGGGTV
                     PAMMGQVKQHTGSFLPGFVVMALFSLLMLAVMRKMQLRWTRTWAEKGGRARSV"
BASE COUNT          504 a          811 c          872 g          454 t
ORIGIN      
        1 gtcctcgttc tgcccgacgg gtagcgcctc ttgcacggca gagacacgcg tggcacccag
       61 gtcgatgatc ttgagcgtgc cctgcgggtc gatcatgatg ttttcaggcc gcaggtcctg
      121 gtgcagcatt tccaggcggt gaaaggcctg cagaccgcgc gcgatctgct ccaccatgcc
      181 gcgcacggtt tccaggtcgg gcgcggggtg gtcgcgcatc cactgcgcca gggtttgccc
      241 ctccaggtac tccagggtga tgtacagcgc ggtgcgtttg cgcgtctgcg cataggggcg
      301 cagcacgtgc gtgttgtcga tgcgccgggc tacccactct tccatcagga agcgctccag
      361 atacgcggtg tcgtcgcgca tgtcgagcga ggggattttg atggcgagct gctgccccgt
      421 ggcttcatcc tgcgccagat acagatggct gcggaaactg gcatgcagtt cgcgcacgat
      481 gcgaaagccg tcgatcaccg tgccgtcaga aggcaaaggc ggaggcagca gggtgacgga
      541 cagggtggag atttctgccg cttcgggggc tggcagttca tccacacgaa cgatctggag
      601 ggtgaggttg tccgcgctgc cgcgtcgcaa cgcttcttcg gtgatggccc gggccgcgcg
      661 gtcgaggtca tcgccggcct gggcaatggc gcccgccaca aaggcgccat ccatgtgctc
      721 gtgcacgccg tcggtggtca gcacaaagat gtcgcccacc tccagcggca gggccaggta
      781 gtccaggtcc agctgctgct gtacgcccag ggcgcggctc aggcagctct tgtcgtgcgt
      841 gacaaacacc cggtggtcgg tggtgagctg ctccagcgcc tggccctgca gcctgcagat
      901 gcgcgcatcg cctatgtgga agaggtgcgc cgtagtggac ttgagcacca gggcgctgaa
      961 ggtgcacaca tagccccggt cccggtcata gcgccactgg ctttgctggg tctgcgcgta
     1021 cagccaggag ttggtggcct ccagcacgcg ctgggccgag cgtttcaccg accaggcatc
     1081 cgacgtgcag tagtagtcgg acaggaagcc cttgaccgcg gattcgctgg ccacatggcc
     1141 cacactgctg ctgctgatgc catcggccag cgcgatggca atgcccttgc cggcgagctg
     1201 cagctcgcgc gggacgcaca ggccatgaaa gtcctggttg gcgtccttgc ggcccttgtc
     1261 gctgtgttgc ccggcggtga ttttcagttg tgtgcgcatg tgggcgcagc ttaacggcgc
     1321 ttgggcgcgg tcttgtagtg ggtggaatac agcgtggcgc cgacaaaggt caggccaccc
     1381 accagattgc ccactacggt ggggatttcg ttccaaatga ggtagtccat gaaggtgaac
     1441 ttgccgccca gcatcaggcc cgaggggaac aggtacatgt tgacgatgct gtgctcaaaa
     1501 cccatgtaga agaacaccag aataggcatc cacatcgcaa tgaccttgcc cgagaccgag
     1561 gtcgacatca tggccgccac cacaccggtg gacaccatcc agttgcacat gacggcgcgg
     1621 ataaacagcg tgagcatgcc ggcagcgccg taggctgcgt agcccacggt gcggccttcg
     1681 ccgatgtggc caattttttg gccaatggcg ttgggggcat cgacaaaacc attggtgaag
     1741 atgatggcca tgaacagggc cacggtgagt gcgcccgcga agttgcccac aaacaccaga
     1801 ctccagttgc gcagcacgcc cgaccaggtg cagccggggc gcttgtccat caccgccagc
     1861 ggtgccaggg taaacacacc ggtcagcagg tcaaagccca gcagatacag aatgcaaaag
     1921 cccaccggaa acagcagcgc gccaatcagc gggttgccgg tgttgacggt aacggacacc
     1981 gcgaacgcgg cggccagcgc caggatggca cccgccatgt aggagcggat caaggtgtcc
     2041 cgggtggaca tgaagatttt ggattctccg gcatccacca ttttggtgac gaattcagat
     2101 gggatgacat aggacatagt ggctttcctg agggttgaga tgaaagaacg gggtggtgtg
     2161 gtggagtcgg gtttagacgc tgcgcgcgcg gccacctttc tccgcccagg tgcgggtcca
     2221 gcgcagttgc atcttgcgca tgacggcgag catcagcagg gagaacagcg ccatcaccac
     2281 aaagccgggc aaaaagctgc cggtgtgttg tttgacctgg cccatcatgg ccggcacggt
     2341 gccgccaccc agggcgccga tttcgccaat catggaaccc gccaccgcag tggaggtggg
     2401 ccaacgcagc ggcaccagct ggaacagcgc accgttgccc gcgcccagtg ccgcaaagca
     2461 caggatcagc agcagggtgg tcatcaccag tgagcccgcc gacaggccga cacccaccag
     2521 ggtcacagcc acggccacca gcaccacggt gagggtgttg acgccgcccc agtggtcgga
     2581 aatccagccg cccgccacgc gcaccacggc gcccatcagt gcggccagca tggtcagctg
     2641 c
//
LOCUS       DAOSJM010000342         2632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_33878_length_2632_cov_4.164144, whole genome shotgun sequence.
ACCESSION   DAOSJM010000342 DAOSJM010000000
VERSION     DAOSJM010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33878_length_2632_cov_4.164144"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PKX82_10970"
     CDS             complement(<1..756)
                     /locus_tag="PKX82_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOF52821.1"
                     /translation="MASSAPEGILIAQRQQGKTTTQCFLRPDKANRHGLITGATGTGK
                     TITLQSLAEGFSHLGVPVFMADVKGDLTGISQKGTLGEKLGKVIAERALPTPEFSAFP
                     TTVWDVFGEQGHPVRATVSDLGPLLLARMLNLNETQAGVLQLVFKIADDDGLLLLDMK
                     DLRAMCQHVGDNASTYTTEYGNISAASIGAIQRGLMQIDQQGGDKFFGEPMLNIADFM
                     QTDGSGKGVVNILAADRLMNAPRLYSTFLLWMLS"
     gene            815..1534
                     /locus_tag="PKX82_10975"
     CDS             815..1534
                     /locus_tag="PKX82_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOF52822.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRGKIWTRIIREITVAARTGGGDPA
                     MNPRLRLAIEKAKAANMPADRVKYNVDKATGSLDGISYEEIRYEGYGIGGAAIIVDTM
                     TDNKVRTVAEVRHAFSKNGGNMGTEGSVAFQFKHCGQLIFAPGTSEDKVMEVALEAGA
                     EDVITDDDGAIEVLTAPGDFEAVKASLEAAGLTAEVAGVTMRAENTIELSGEDAERMQ
                     KLLDALEDLDDTQDIYHNAAL"
     gene            1541..>2632
                     /gene="purD"
                     /locus_tag="PKX82_10980"
     CDS             1541..>2632
                     /gene="purD"
                     /locus_tag="PKX82_10980"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOF52823.1"
                     /translation="MNVLVIGGGGREHALAWKLAQSPKVRTIYVAPGNGGTATDSRLQ
                     NVPITDVVELRTWAQENKITLTVVGPEVPLAAGVVDEFRAHGLRIFGPTKAAAQLESS
                     KAFSKAFMRRHGIPTAEYATFSDPVAAHAYVDKMGAPTVVKADGLAAGKGVVVAMTLA
                     EAHEAIDFMLVDDTLGVAHNDGADGVAVPRVVIEEFLDGEEASFIVMVDGKNVLPLAT
                     SQDHKRLQDGDQGPNTGGMGAYSPAPVVTPDIHAKAMREIILPTVKGMEADGIPFSGF
                     LYAGLMIDKNGQPKTLEFNCRMGDPETQPIMMRLKTDLVDVLMAATEPGPHGKLDEVE
                     LQWDRRTALGVVLAAHGYPLNPRKGDVITG"
BASE COUNT          544 a          764 c          860 g          464 t
ORIGIN      
        1 gcttagcatc cacagcagga aggtggagta caggcgcggc gcattcatca ggcggtcggc
       61 ggccaggatg ttgaccacgc ccttgccgct gccatcggtc tgcatgaagt cggcaatgtt
      121 gagcatcggc tcgccgaaaa acttgtcgcc gccttgctgg tcgatctgca tcagcccgcg
      181 ctggatggcg ccaatactgg cggcgctgat gttgccgtac tcggtggtgt aggtggatgc
      241 gttgtcgccc acatgctggc acatggcgcg caggtctttc atgtcgagca gcagcaggcc
      301 atcgtcgtcg gcaatcttga acaccagttg cagcacgccg gcctgggttt cgttcaggtt
      361 gagcatgcgc gcgagtagca gggggccgag gtcactgacg gtagcgcgca ccgggtggcc
      421 ctgttcgcca aacacatccc agaccgtggt ggggaaggcg gagaactccg gggttggtaa
      481 cgcccgttct gcgatgactt tgcccagttt ttcacctaaa gtgccctttt gggagattcc
      541 agtcaggtca cccttgacgt cggccatgaa caccggcacg cccaggtggg aaaaaccctc
      601 tgccagggat tgcagggtga tggtcttgcc ggtgccggtg gcgcccgtga tgaggccatg
      661 gcgattggcc ttgtcggggc gcaaaaagca ctgggtggtg gttttgcctt gctggcgttg
      721 ggcaatgagg atgccttcag gtgcgctgga tgccatggaa gtcccgaaaa gtacaatcaa
      781 gccaatagcg taacgaattt ttgaaggatt tcgcgtggca ggacacagca aatgggccaa
      841 tattcagcac cgcaaggggc gacaggacga gaagcggggc aagatctgga cccgcatcat
      901 ccgtgaaatc acggtagcgg cccgcactgg tggtggcgac ccggcgatga acccccgcct
      961 gcgcctggcc attgaaaagg ccaaggcggc caacatgcct gccgaccgcg tgaaatacaa
     1021 cgtggacaag gccaccggat cactggatgg catcagctac gaggaaatcc gctacgaagg
     1081 ctatggcatt ggtggtgcag cgatcatcgt ggacacgatg accgacaaca aggtgcgcac
     1141 cgtggccgaa gtgcgccacg ccttcagcaa gaatggcggc aacatgggca ccgaaggctc
     1201 ggtggccttc cagttcaagc actgcggcca actcattttt gcaccgggca ccagcgaaga
     1261 caaggtcatg gaagtggctc tggaagctgg cgccgaagac gtcatcacgg acgacgacgg
     1321 tgcgatcgag gtgctgaccg cacctggcga ctttgaggcg gtgaaagcgt ccctggaagc
     1381 tgctggcctc acggccgaag tggcgggcgt gactatgcgt gccgaaaaca ccatcgaact
     1441 gagcggcgag gatgccgaac gcatgcagaa actgctggac gctctggaag acctcgacga
     1501 cacccaagat atttatcaca acgcggcgct gtaacccctt atgaacgtac tagtaattgg
     1561 cggcggtggt cgcgaacatg cattggcctg gaaactggcc cagtctccca aggtccgtac
     1621 catttatgtg gcgcccggca acggtggtac cgcgacggat tcccgtctgc agaatgtgcc
     1681 gattaccgat gtggtggagt tgcgcacctg ggcgcaggag aacaagatca cgctgacggt
     1741 ggtgggcccc gaagtgccgc tggccgctgg tgtggtcgac gaattccgcg cccacggtct
     1801 gcgcattttt ggcccgacca aggctgcagc acaactggaa agctccaagg cgttcagcaa
     1861 ggccttcatg cgccgccacg gcatccccac agcggagtac gccacctttt cggaccccgt
     1921 tgcagcacac gcttatgtag acaagatggg ggcacccacc gtggtcaagg ccgacgggct
     1981 ggccgccgga aagggcgtgg tggtggccat gaccctggct gaggcccacg aagccatcga
     2041 cttcatgctg gtggacgaca ccctgggggt ggcgcataac gacggcgcgg atggggtggc
     2101 cgtgcctcgc gtggtgattg aagagtttct ggacggtgaa gaggccagtt tcatcgtcat
     2161 ggtggacggc aagaatgtgc tgccattggc caccagccag gaccacaagc gcctgcagga
     2221 cggcgaccaa ggccccaaca ccggtggcat gggcgcctac tcgcctgcgc ctgtggtgac
     2281 gcccgacatc cacgccaagg ccatgcgcga aatcattctg cccaccgtca agggcatgga
     2341 agccgacggc attcccttca gcggctttct gtatgccggc ctgatgatcg acaagaacgg
     2401 ccagcccaag acgctggagt tcaactgccg catgggcgac cccgaaacgc agcccatcat
     2461 gatgcgcctc aagaccgatc tggtggatgt gctgatggcg gcgaccgagc cgggacccca
     2521 cggcaagctg gacgaagtgg agttgcagtg ggaccgccgc accgcgctgg gcgtggtgct
     2581 ggcagcgcac ggctacccgc tgaatccgcg caaaggcgat gtcattaccg gt
//
LOCUS       DAOSJM010000343         2631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_33893_length_2631_cov_3.980978, whole genome shotgun sequence.
ACCESSION   DAOSJM010000343 DAOSJM010000000
VERSION     DAOSJM010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33893_length_2631_cov_3.980978"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PKX82_10985"
     CDS             <1..268
                     /locus_tag="PKX82_10985"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOF52824.1"
                     /translation="LKGVTGSGALDTDAVEAMLPRIRQHISTPVGVGFGIRDAATAKT
                     ISRVSDAVVIGSKIIQLLEDQPRDKVAATAQQFLREIRTAMDQG"
     gene            343..1215
                     /gene="accD"
                     /locus_tag="PKX82_10990"
     CDS             343..1215
                     /gene="accD"
                     /locus_tag="PKX82_10990"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOF52825.1"
                     /translation="MSWLEKLLPPKIQHTDPTERRSVPEGLWIKCPSCEAVLYKTDLE
                     QNQNVCPTCSHHHRIGARARLNVFLDNEGRFEIGQEVLPVDALKFKDSRKYPERLKEA
                     LENTGETDALVVMGGAVHGVSVVAACFEFEFMGGSMGSVVGERFVRGVETAIEQKVPF
                     ICFTATGGARMQEGLLSLMQMAKTNASLTRLAKKGLPYISVLTDPTMGGVSAGFAFLG
                     DVVIAEPKALIGFAGPRVIESTVRVTLPEGFQRAEFLQTKGAIDFICDRRELRKTIAD
                     TLAMLQRQPADAVI"
     gene            complement(1235..1804)
                     /locus_tag="PKX82_10995"
     CDS             complement(1235..1804)
                     /locus_tag="PKX82_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOF52826.1"
                     /translation="MLYQIVSLLLEVAAGLIAGVCLLRLYMQYQRIPISARSGNPMGK
                     FIFALTDWLVLPLRRVVPAVGRWDMASLVAAFLVQLAQFCILWVLTGMAASLASVAVL
                     AVFGLVRMAISGLTGLVIVYAILSWVQTQSYMADLLERLVMPVLVPIRRVLPLVGGVD
                     LSPLVLLVLLQIAAIVLGNVQAAVLVAVA"
     gene            1900..>2631
                     /locus_tag="PKX82_11000"
     CDS             1900..>2631
                     /locus_tag="PKX82_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HOF52827.1"
                     /translation="MSDHTPAAPAPQDENQLILERREKLKALRQIQADGGAVAFPNDF
                     KPSHKAEQLFDHYQHKSTEELATINAHAKVAGRMMLKRVMGKASFCTLQDASFGSSAG
                     RIQIYVKREDVGDEVYESFKHWDLGDIVAAEGVVFKTKTGELSLHASKIRLLTKSLRP
                     MPDKFHGINDQEVKYRQRYIDLMTDEDTRRRFVARSKAVSGLREFMVQHGFLEVETPM
                     LHPIPGGANAKPFTTHHNALDQQMFL"
BASE COUNT          586 a          840 c          769 g          436 t
ORIGIN      
        1 gctcaagggc gtgaccggct ccggcgcgct cgataccgac gccgtggagg ccatgctgcc
       61 gcgcatccgc cagcacatca gcacgccggt cggcgtgggc tttggcatcc gcgacgcggc
      121 taccgccaaa accatctcac gcgtgtccga tgcggtggtg attggcagca agatcatcca
      181 gttgctggaa gaccagccgc gcgacaaggt ggcagctacg gcgcaacagt tcttgcgcga
      241 gattcgcacc gccatggacc aaggctgaac gaccgaggtt tagccgataa tcgccccctg
      301 accgttggac gccctgttgg gcattgcact ggagatttgc acatgagttg gcttgaaaaa
      361 ctgctacccc cgaaaattca gcacaccgac cccaccgagc gtcgcagcgt gcccgaaggc
      421 ctgtggatca agtgccccag ctgcgaagcg gtgctctaca agaccgatct ggagcaaaac
      481 cagaacgtct gccccacctg tagccaccac catcgtattg gcgcccgtgc gcgcctgaat
      541 gtgtttctgg acaacgaagg ccgcttcgaa attggacagg aagtgctacc ggtcgatgcc
      601 ctgaaattca aggacagccg caaataccct gagcgcctca aggaagccct ggaaaacact
      661 ggagaaaccg atgccctggt ggtcatgggt ggtgcagtgc atggcgtgag cgtggtggca
      721 gcctgcttcg aatttgaatt catgggcggc agcatgggat cggtcgtagg cgagcgtttt
      781 gtgcgcggcg tcgaaactgc gattgaacag aaagtgccct tcatctgctt tactgccacc
      841 ggtggcgcgc gcatgcagga aggcctgctg tccctgatgc agatggcaaa gaccaatgca
      901 tctttgaccc gtttggccaa aaagggcctg ccctacatca gcgtgctgac cgacccgacc
      961 atgggcggcg tgagcgccgg ttttgcgttc ctgggcgacg tggtgattgc cgagcccaag
     1021 gccctgattg gctttgcggg cccccgcgtg atcgaatcca ccgtgcgcgt gacactgcct
     1081 gaaggttttc agcgtgcaga gtttctgcaa accaagggtg cgatcgactt tatctgcgac
     1141 cgccgggaac tgcgcaaaac catcgccgac acgctggcca tgctgcagcg ccagcctgcc
     1201 gacgcagtga tctgatctgg ctgcagctgg cctgctaggc cactgccacc agcacagcag
     1261 cctgcacgtt gcccagcaca atcgcggcaa tctgcagcag caccagcagc accaagggtg
     1321 acaggtccac gccgccgacc agcggcagca cacgccggat cggcaccagc acgggcatca
     1381 ccagacgctc cagcagatcg gccatgtagg actgggtttg cacccacgac aggatggcgt
     1441 acacgatcac caggccggtc aggccagaga ttgccatacg caccagacca aataccgcca
     1501 ggacggccac cgaagccagg ctggccgcca tgccggtcag cacccacaaa atacaaaact
     1561 gcgccagctg caccaaaaat gcggctacca ggctggccat atcccagcgc cccacggcag
     1621 gcaccacgcg ccgcagcggc aacaccagcc agtcggtcag ggcaaatatg aacttgccca
     1681 tggggttgcc cgagcgggct gatatgggaa tgcgctggta ctgcatgtac aggcgcaaca
     1741 ggcacacgcc agcgatcagg ccagcggcca cttccagcaa aagggagacg atttggtaga
     1801 gcatggtttt gaatgtggga ggcccgcgca tcatagccgc tgaccagcgg gtggcgtccc
     1861 tcgtagaatc gagggttttg gcccgcagaa ttacctccca tgtctgacca cacacccgcc
     1921 gccccagccc cccaggacga aaaccagctg attctggagc gccgcgaaaa actcaaggcc
     1981 ctgcgccaga tccaggccga tggcggtgcc gtggccttcc ccaacgactt caagcccagc
     2041 cacaaggccg aacagctctt tgaccactac cagcacaaga gcaccgaaga actagccacc
     2101 atcaatgcac atgccaaggt cgccggccgc atgatgctca agcgggtgat gggcaaggcc
     2161 agcttctgca ccttgcagga tgcgtcgttt ggcagcagcg cgggtcgcat ccagatctac
     2221 gtcaagcgcg aggacgtggg cgacgaagtg tatgaatcct tcaagcactg ggatctgggc
     2281 gacatcgtgg cagccgaagg tgtggtgttc aagaccaaga ccggcgaact gtccctccac
     2341 gcttcaaaga tccgcctgct caccaagagc ctgcggccca tgcctgacaa gtttcacggc
     2401 atcaatgacc aggaagtcaa gtaccgccag cgctacatcg acctgatgac cgatgaggac
     2461 acacgccggc gttttgtagc ccgcagcaaa gccgtgagtg gtctgcgtga gttcatggtg
     2521 cagcacggtt ttctggaagt ggaaaccccc atgctccacc ccatccccgg tggtgccaac
     2581 gccaagccat tcaccacgca ccacaacgcg ctggaccagc agatgttcct g
//
LOCUS       DAOSJM010000344         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_33969_length_2626_cov_3.721120, whole genome shotgun sequence.
ACCESSION   DAOSJM010000344 DAOSJM010000000
VERSION     DAOSJM010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33969_length_2626_cov_3.721120"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKX82_11005"
     CDS             <1..457
                     /locus_tag="PKX82_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOF52828.1"
                     /translation="AALTAALGRAPRIGVAGLNPHAGEGGLFGREEIEVIAPAMAAAR
                     AQGCDVSGPWAPDTVFMRARNGAGHPGEFDVVVAQYHDQGLIPVKYLGVEKGVNVTLG
                     LPLVRTSPDHGTAFDIAGTGSADASSLLEAVRMARSLSNSPPERSRLTS"
     gene            complement(447..875)
                     /gene="mscL"
                     /locus_tag="PKX82_11010"
     CDS             complement(447..875)
                     /gene="mscL"
                     /locus_tag="PKX82_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOF52829.1"
                     /translation="MGMMQEFKEFAVKGNVVDLAVGVIIGGAFGKIVDSVVGDLIMPL
                     VGAIIGKLDFSNLYVALGAIPAGTENTLAALKKAGVPVFAYGSFITVAVNFAILAFII
                     FLMIKQINRLKKEEPAAPAAAPVTPEDVVLLREIRDSLKK"
     gene            1029..1643
                     /gene="petA"
                     /locus_tag="PKX82_11015"
     CDS             1029..1643
                     /gene="petA"
                     /locus_tag="PKX82_11015"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HOF52830.1"
                     /translation="MSETLVGTQPIDSSKRTWLIASGCAGAVGGVATAIPFVSTFQPS
                     ERAKAAGAAVEVDIAGLKPGEKVTVEWRGKPVWVVRRTPEQLAALGKVTDQLADPESK
                     RKPDEFTPVYARNEGRSIKPEFFVAVGICTHLGCSPSDKFQTGAQPSLPDDWQGGFLC
                     PCHGSTFDMAGRVFKNKPAPDNLEIPPHMYLSDSKLLIGEDKKA"
     gene            1667..>2626
                     /locus_tag="PKX82_11020"
     CDS             1667..>2626
                     /locus_tag="PKX82_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HOF52831.1"
                     /translation="MAEFKDISPNASAGAKLTNWFENRFPTAFDAYKVHMSEYYAPKN
                     FNFWYIFGSLALLVLVIQIVTGIFLVMHYKPDAALAFGSVEYIMRDVPWGWLIRYMHS
                     TGASAFFVVVYLHMYRGLIYGSYRKPRELVWVFGCAIFLALMAEAFMGYLLPWGQMSY
                     WGAQVIVNLFAAIPFVGPDLALLIRGDYVVGDATLNRFFSFHVIAVPLVLLGLVVAHL
                     LALHDVGSNNPDGVEIKGPNAPRDAQGHPLDGIPFHPYYTVHDIFGVSVFLMVFTAII
                     FFAPEFGGYFLEYNNFIPADPLKTPLHIAPVWYFTPFYSMLRAI"
BASE COUNT          487 a          761 c          773 g          605 t
ORIGIN      
        1 tgcggccctg acggccgcgt tggggcgtgc gccccggatt ggcgtggcgg ggctgaaccc
       61 gcatgcgggc gagggcgggc tgttcgggcg tgaagaaatt gaagtgatag cgccagcgat
      121 ggctgcagcg cgagcacagg ggtgtgatgt gtcaggcccc tgggcaccgg atacggtgtt
      181 catgcgcgcc cgcaacggcg ccggacatcc gggcgagttt gatgtggtcg tggcgcagta
      241 ccacgatcag gggctgatcc cggtgaaata cctgggggtg gagaagggtg tcaatgtcac
      301 cctgggtctg ccactggtgc gcaccagtcc ggaccacgga accgcatttg atattgcggg
      361 aaccggcagc gccgatgcgt ccagcctgct ggaggctgtc cgcatggcgc ggagtctgag
      421 caattccccg ccggagcgga gcaggcttac ttcttgaggc tgtcgcggat ttcgcgcagc
      481 aagacgacgt cttcgggtgt caccggtgcg gcggcaggtg ctgcgggctc ttccttcttc
      541 aggcggttga tctgtttgat catcaggaag atgatgaacg ccagaatggc gaagttcacc
      601 gccacggtga tgaaactgcc ataggcaaac acgggaacgc cagctttctt aagggctgcc
      661 agggtgtttt ctgtgccggc agggatagcg cccaatgcaa cgtacaggtt ggaaaagtcc
      721 agttttccaa taattgcgcc gaccagcggc atgatcaggt cgccgaccac cgagtccaca
      781 atcttcccaa aggcgccacc gatgatcacg ccgacggcca gatcgaccac attgcccttg
      841 acggcaaatt ccttgaattc ctgcatcatt cccatttggt gatcctttgt gagttgttga
      901 tagttcgtct attgtccaca aaaacaaggg gtttaacttc aacaaccggg cgcaccgcgt
      961 tgaatactcc tatggccgtc agctacaatc gggtcttacc cccgtagtga tgttcattga
     1021 ggattctcat gagtgaaacc cttgtcggca cccagccgat cgactccagc aagcgcacgt
     1081 ggttgattgc ttccggatgc gcaggcgccg tcggtggcgt tgcaaccgca attcccttcg
     1141 tcagcacttt ccagccttcc gagcgtgcca aggccgccgg tgccgccgtt gaggtggata
     1201 ttgccggact caagcccggc gaaaaggtga ccgtcgaatg gcgtggcaag cccgtgtggg
     1261 tcgttcgtcg cacaccggag cagcttgcag ccctgggcaa ggtgacagac cagctggcag
     1321 accctgaatc caagcgcaag cctgacgaat tcactcctgt ctacgcccgt aatgaaggcc
     1381 gctccatcaa gcccgagttt tttgtcgctg ttggcatctg cacccatctg ggttgctcgc
     1441 cttcggataa gtttcagacg ggtgcccagc cctccctgcc tgatgactgg caaggcggtt
     1501 tcctgtgccc ctgccatggt tcgactttcg acatggcagg ccgcgttttc aagaacaagc
     1561 ctgctccaga caatctggaa attcccccgc acatgtacct ctctgacagc aagttgctga
     1621 tcggtgaaga caaaaaagcc tgacctagaa gcgtacggag aaacacatgg ctgaattcaa
     1681 ggatatttcc cccaacgctt cggcgggcgc caagctcacc aactggtttg agaaccgctt
     1741 tcccacagcg tttgatgcct acaaggtcca tatgtcggag tactacgctc cgaagaactt
     1801 caatttctgg tacatctttg gctcgctggc gttgctggtg ctggtcatcc agatcgtgac
     1861 cggcattttc ctggtgatgc actacaaacc cgatgcggct ctggccttcg gttcggtgga
     1921 atacatcatg cgcgatgtgc catggggttg gttgatccgc tacatgcact ccacaggcgc
     1981 ttctgccttt tttgtggtgg tctatctgca catgtaccgt ggcctgattt acggtagcta
     2041 ccgcaagccg cgcgagctgg tctgggtgtt tggctgcgcg attttcctgg cgttgatggc
     2101 cgaagctttc atgggttacc tgctgccctg gggccagatg agctactggg gtgcccaggt
     2161 gatcgtgaac ctgtttgccg ccattccgtt tgtgggccca gatctggctt tgctgatccg
     2221 tggtgactac gtggtgggtg acgcaacact gaaccgcttc ttcagcttcc acgtgattgc
     2281 tgtgccgctg gtgctgctgg gtctggtggt tgcgcacttg ctggcgctgc acgatgtggg
     2341 ttccaacaac cccgatggcg tggaaatcaa gggccccaac gctccccgcg atgcccaggg
     2401 tcatcctctg gacggtattc cgttccaccc ctactacacc gtgcacgata tcttcggtgt
     2461 cagcgtgttc ctgatggttt tcaccgccat catcttcttc gcgcccgagt tcggcggtta
     2521 cttcctggag tacaacaact tcatcccggc cgatccgctg aagacgccgc tgcacattgc
     2581 accggtctgg tacttcacgc cgttctactc gatgctgcgt gccatt
//
LOCUS       DAOSJM010000345         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_34090_length_2619_cov_3.801482, whole genome shotgun sequence.
ACCESSION   DAOSJM010000345 DAOSJM010000000
VERSION     DAOSJM010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34090_length_2619_cov_3.801482"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="PKX82_11025"
     CDS             complement(<1..876)
                     /locus_tag="PKX82_11025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOF52832.1"
                     /translation="MTRPSILVTRAIFPEVLDHLRQHFEVEANQADDAWSADELAQRL
                     QGKAGAFTSGADPITAALLARCPGLKICANMAVGYNNFDVPAMTAAGVLATNAPDVLT
                     ETTADFGFALLMATARRVTESEHYLRAGKWTRWAYDMFSGCEVHGSTLGIIGMGRIGQ
                     GIAKRGALGFGMKVIYHNRSRLDDATEGQCRATYVGKEALLRTADHVVLVVPYSKESH
                     HTIGAAELAQMKPTATLVNIARGGIVDDAALAVALRDKRIAAAGLDVFEGEPKVHPDL
                     LTVPNVVLTPHIASAT"
     gene            complement(900..2108)
                     /gene="pncB"
                     /locus_tag="PKX82_11030"
     CDS             complement(900..2108)
                     /gene="pncB"
                     /locus_tag="PKX82_11030"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOF52833.1"
                     /translation="MIITSLLDTDLYKFTMMQVVLHQFPGAQVEYRFKCRNAGINLAE
                     HAQEIREEIRALCSLQFQESELAYLRSMRFIKSDFVDFLGLFRLNEKYITVTPQPSGE
                     LEIVIIGPWLHTILFEIPVLAIVNEVYFRNTQKVPDFVEGRKRLDAKIAQLHGPDLKD
                     LKIADYGTRRRFSRAWHEEVMRVLSAKLGTVQSPSRGKGSPGQLAGTSNVELAHKLGL
                     IPLGTMAHEYLQASQALGPRLRDSQIFAFESWAREYRGDLGIALSDVYGMSAFLRDFD
                     LYFCKLFDGARHDSGDPFAWGERMLDHYVRNRVDPRTKTLIFSDGLTVPRTIELYNQF
                     RGRCQLAFGIGTNLTNDLGYEPLQIVIKMVRCNGQPVAKLSDTPSKNMCDDEKYLAYL
                     RQVFDIPQPH"
     gene            complement(2112..>2619)
                     /locus_tag="PKX82_11035"
     CDS             complement(2112..>2619)
                     /locus_tag="PKX82_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52834.1"
                     /translation="ATGAVSYQNKSILGDEPHHIARLGLGYVPEDRRLFTGLTVQENL
                     LIAARFGPAAAGAASHTTLRAQQDHVLHLFPALQGMLARKAAHMSGGEQQMLSIARTL
                     MTGPSLLLLDEPCEGIAPVLVESIRDALLQLKGEGMPMLVAEQNAILASRADRVITLV
                     AGQIAQPV"
BASE COUNT          494 a          810 c          809 g          506 t
ORIGIN      
        1 cgtggcgctg gcgatgtgcg gtgtcagcac cacattgggc accgtcagca gatccgggtg
       61 gaccttgggc tcgccttcga acacatccag tccggcggct gcaatgcgtt tgtcgcgcaa
      121 ggcgaccgcc agtgctgcgt catccacaat gccgccccgt gcaatgttca ccagcgtggc
      181 ggtcggcttc atctgggcca gctcggctgc gccaatggtg tggtgggact ctttcgaata
      241 gggcacgacc agcaccacat ggtcggccgt gcgcagcaac gcttccttgc ccacataggt
      301 cgcgcggcat tggccttcag tcgcgtcatc cagcctgctg cggttgtggt aaatcacctt
      361 catgccaaag cccagcgccc cgcgtttggc aatgccctgc ccgatgcgcc ccatgccgat
      421 gatgcccagc gtggagccat gtacctcgca gcccgagaac atgtcatagg cccagcgcgt
      481 ccatttgccg gcacgcaggt aatgttcgct ctcggtcaca cgccgcgcgg tggccatgag
      541 cagcgcaaaa ccgaagtcgg cggtggtctc ggtcaataca tcgggggcat tggtggccag
      601 cacacccgcg gcggtcatgg ctggcacatc gaagttgttg taacccaccg ccatgttggc
      661 gcagattttc agcccggggc atcgcgccag caaggctgcg gtgatcgggt ctgcaccgct
      721 ggtgaaagca ccggccttgc cctgcaaacg ctgcgcgagt tcatcggcac tccaggcgtc
      781 atcggcctgg ttggcttcga cctcgaaatg ctgacgcaga tgatcgagca cctcgggaaa
      841 gatggcgcgg gtcaccagaa tggaaggtcg ggtcatggct aaaactccag ggtaaaacgt
      901 cagtgcggct gcggaatgtc gaacacctgg cgcaggtagg ccaggtactt ctcgtcatcg
      961 cacatattct tggatggcgt atccgacagc ttggccacgg gctggccatt gcagcgcacc
     1021 atcttgatga cgatctgcaa gggctcgtag cccaaatcgt tggtgaggtt ggtaccaata
     1081 ccaaaggcca gctggcagcg tccgcggaac tggttgtaca gctcgatggt gcgtggcacc
     1141 gtcaggccgt cgctgaagat gagtgtcttg gtgcgcggat cgacccggtt gcgcacatag
     1201 tggtccagca tgcgctcgcc ccaggcaaag gggtcgccac tgtcatggcg ggcgccgtcg
     1261 aacagtttgc agaaatacag gtcaaagtcg cgcaggaacg cgctcatgcc atagacatcg
     1321 ctcagcgcaa tacccaggtc gccccggtac tcccgagccc aggactcgaa ggcaaaaatc
     1381 tggctgtcgc gcagacgcgg gcccaacgcc tgcgaggcct gcaaatactc gtgggccatg
     1441 gtgcccagcg gaatcagccc cagcttgtgt gccagctcca cattactggt acctgccagc
     1501 tgacccggtg aacccttgcc gcggctcgga ctctgaacgg ttcccagctt ggctgagagc
     1561 acccgcatga cttcttcgtg ccaggcgcgc gagaagcgcc ggcgggtacc gtagtcggca
     1621 atcttcaggt ctttcaaatc aggaccgtgc aactgcgcga tcttggcgtc cagccgtttg
     1681 cggccctcca cgaagtcggg cactttctgc gtgttgcgga aatacacctc gttgacgatg
     1741 gccagcactg ggatctcgaa caggatggtg tgcagccagg gcccaatgat cacgatttca
     1801 agctcgcccg agggctgtgg cgtgacggtg atgtattttt cgttaagcct gaacagaccc
     1861 agaaagtcta caaaatcgct cttgatgaaa cgcatggacc gcaagtaggc cagttcgctt
     1921 tcctggaact gcaggctgca cagcgcgcgg atttcctcgc ggatttcctg cgcatgctcc
     1981 gccaggttga tgcccgcgtt gcggcacttg aagcggtact ccacctgcgc acccgggaac
     2041 tggtgcagca ccacctgcat catggtgaac ttgtacaggt cggtgtcgag cagactggta
     2101 atgatcatgg ttcaaacggg ttgggctatc tgtccggcta cgagtgtaat gacccggtcg
     2161 gcccggctgg ccagaatggc gttttgctct gcgaccagca tgggcatgcc ctcgcccttg
     2221 agctgtagca gcgcatcgcg gatggattcc accagcaccg gcgcgatgcc ctcgcagggc
     2281 tcatcaagca gcagcagcga cgggccggtc atcagcgtgc gggcgatgct gagcatctgc
     2341 tgctcgccgc cgctcatatg cgcggccttg cgggccaaca tgccctggag cgccgggaac
     2401 agatgcaaga catggtcctg ctgcgcacgc agcgtggtgt ggctggccgc acccgccgct
     2461 gcaggaccga accgggctgc gatcagcagg ttttcctgca ccgtcaggcc ggtgaacaga
     2521 cgccggtctt ccggcacata acccagcccc agacgtgcaa tatggtgcgg ctcgtcaccc
     2581 agaatgctct tgttttgata gctgactgcg cccgtggca
//
LOCUS       DAOSJM010000346         2616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_34148_length_2616_cov_2.559157, whole genome shotgun sequence.
ACCESSION   DAOSJM010000346 DAOSJM010000000
VERSION     DAOSJM010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34148_length_2616_cov_2.559157"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKX82_11040"
     CDS             complement(<1..370)
                     /locus_tag="PKX82_11040"
                     /inference="COORDINATES: protein motif:HMM:NF022458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HOF52835.1"
                     /translation="MLRPLASLIIASVLTMPALEVYAALGRPPSTLAGNVSHPVPAAV
                     ARRLAATAPMAPNLYTVQETQLESRTVVREYATPAGQVFAVTWKGPVLPNLQTLLGGY
                     FPAFEQETAQARQAGRQRSRV"
     gene            501..1235
                     /gene="imuA"
                     /locus_tag="PKX82_11045"
     CDS             501..1235
                     /gene="imuA"
                     /locus_tag="PKX82_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HOF52836.1"
                     /translation="MPSLAHTPSLADIHADVWQAHALATAPVRVQATGNGALDAQLPG
                     GGWPVGGMTEVLQIDGAHCEWRLLLPALARCGQGPVVLVGAPFVPFAPALAAQGLAVQ
                     RLLWMAAQTLAQRLWAAEQALRCAKVDAVLAWLPAGRADTVRTDQLRRLQMAASGYNK
                     LLFVMRPAAAWADASPAVLRLQVGVDSQRADALEVQLLKRRGPPCDQPLTLQACSAQL
                     SMLLAVHGQPLVRPRDALDRTAARAA"
     gene            1201..2538
                     /locus_tag="PKX82_11050"
     CDS             1201..2538
                     /locus_tag="PKX82_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOF52837.1"
                     /translation="MHWIALQPAPHDGAADAPALTDVHAALAWWALQFTPLVARVQDV
                     LVLEVSGSERLFGGRTALLAQLFKPNRPVAPVYSAQGATSLIAIGRLRVAAAGRPVDL
                     APDALPLQALAAAQAHLPTLLRLGCRTWGQLRALPRGGLARRFGAGLVDALDRAYGLQ
                     PEVYPWLTLPDVFDAPLELQARVESAPALLFGARRLLAQLLVWLRARQRGVLVLELLW
                     ELDAARSAEKQGRLELRTAQATQDMQHLQRLLGEQLARVTLPAPVLYLRLRTLQTQPL
                     GGESHSLLPDDLRHGDSLHQMAERVAARLGATQVLCVQTRADHRPEQMQCWQPWVAFD
                     GKVDAIKKRANEAVQTRDEGLNAHASLYPSWLLPVPQRLAVRRGQPQHHGDLALLAGP
                     QRVEAGWLDGVAALRDYYVAHSAGVGLVWIYRERLTPNPGEDHAPWYLHGLFA"
BASE COUNT          404 a          794 c          931 g          487 t
ORIGIN      
        1 gaacccgtga gcgctgcctt ccggcttgtc gtgcctgggc ggtctcctgc tcaaaggccg
       61 ggaaatatcc gccgagcagg gtttgcaggt tggggagcac cggccctttc caggtcactg
      121 caaatacctg tccggcaggt gtggcgtatt cacgtacgac ggtgcggctc tccagctggg
      181 tttcctgcac ggtgtacaga ttgggcgcca taggtgcagt ggccgccagg cgccgcgcga
      241 cggctgccgg taccgggtgg ctgacgttcc ccgccaatgt ggaaggtggc cgccccagag
      301 ctgcatagac ttcgagggcg ggcattgtca gcacgctggc aatgatcagg ctggcaaggg
      361 gacgcaacat ggggatgact gtaccagccg aatacggtac ggcggccata aaaaggtaag
      421 taaaaaattc atccgctggc gcagctgagg caagcgattt atactgtatt taactacagc
      481 tttattcgca aacccttacc atgccttcgc ttgcgcatac cccctctttg gccgacatcc
      541 atgccgatgt ctggcaagcg cacgccctgg ccacggcgcc cgtccgggta caggcgacgg
      601 gaaatggggc gctggatgca caacttcctg gtggtggttg gccggtgggc ggcatgaccg
      661 aggtgcttca gatcgacggg gcgcactgcg aatggcgcct gctgctaccg gctctggcgc
      721 gttgtgggca ggggccggtg gttctggtgg gggcaccgtt tgtaccgttt gcgcctgccc
      781 tggcggcaca gggcctggcg gtgcagcggc tgctgtggat ggctgcccag acgctggcgc
      841 agcgcctgtg ggcggcagag caggccctgc ggtgtgccaa ggtggatgca gtgctggcct
      901 ggttgcccgc gggacgtgcg gatacggtgc gcaccgacca gttgcggcgc ctgcagatgg
      961 ctgcgtccgg gtacaacaaa cttttgtttg tcatgcggcc cgctgctgca tgggccgacg
     1021 catcccccgc tgtactgcgc ttgcaggtgg gggtggattc gcagcgggcc gatgcactgg
     1081 aagtgcaact gctcaagcgc cgtggcccgc cgtgtgatca gccgctgaca ctgcaggcat
     1141 gctccgcgca gctttccatg ttgctggcgg tccacggcca gcctcttgtg aggccacgcg
     1201 atgcactgga tcgcactgca gcccgcgccg catgacggtg ccgcagacgc ccccgcgctg
     1261 accgatgtgc atgcggcgct ggcgtggtgg gcgctgcagt tcaccccgct ggtggcacgg
     1321 gtgcaggacg tgctggtgct ggaggtctct ggcagcgagc ggctgtttgg tggccggaca
     1381 gctcttctgg cgcagttatt taagccaaat cgacctgtag cccccgtata cagtgcgcaa
     1441 ggtgctacat ctttaatagc aataggacgt ttgcgtgtgg ccgcggccgg gcggcctgtg
     1501 gatctggcgc ctgacgcctt gccgttgcag gcactggcgg ctgcacaagc ccatttgccc
     1561 acgcttctgc gtctgggctg tcgtacctgg gggcaattgc gtgcattgcc gcggggggga
     1621 ttggcgcgcc ggtttggcgc cgggctggtg gacgcgctgg accgcgccta tgggctgcag
     1681 cccgaggtgt atccgtggct gaccttgccc gatgtgtttg atgccccgct ggagttgcag
     1741 gccagggtgg agtcggcacc ggctttgctc tttggtgccc gccgcctgct ggcccagttg
     1801 ctggtgtggc tgcgcgcccg ccagcgcggt gtgctggtgc tggagctgct gtgggaactg
     1861 gatgccgccc gcagcgctga gaagcaaggc cgtctggagt tgcgcacggc acaagccacg
     1921 caggacatgc agcacctgca gcggctgctg ggtgagcagc tggcccgcgt caccttgcct
     1981 gcaccggtgc tgtacctgcg cttgcgcaca ttgcagaccc agcccttggg cggggagtcg
     2041 cacagcctgt tgcccgacga cctgcgccac ggcgacagcc tgcaccagat ggccgagcgc
     2101 gtggcggccc ggctgggtgc gacgcaggtg ctgtgtgtcc agacgcgggc agaccaccgg
     2161 ccggaacaga tgcagtgctg gcagccctgg gtagcgttcg atggcaaggt agatgctata
     2221 aaaaagagag ctaacgaagc agtgcagacg agggacgaag gcttaaacgc ccatgcatcg
     2281 ctatacccct cctggctact gccggtaccg cagcggctgg cggtgcgcag aggccagcca
     2341 cagcaccatg gcgacctggc cctgctggcc gggccgcagc gggtcgaggc cggctggctc
     2401 gacggggtgg ctgcactgcg cgactattac gtggcgcaca gtgccggggt ggggctggta
     2461 tggatctacc gcgaacgcct gacgcccaac ccgggggaag accatgcccc ctggtacctg
     2521 catgggctgt ttgcctgaat gcagggggtc agcccggagg gggcggcgga atcagtttgc
     2581 cgccccctcc gggctgaccc cctgcattca ggcaaa
//
LOCUS       DAOSJM010000347         2615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_34158_length_2615_cov_4.339062, whole genome shotgun sequence.
ACCESSION   DAOSJM010000347 DAOSJM010000000
VERSION     DAOSJM010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34158_length_2615_cov_4.339062"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..658
                     /locus_tag="PKX82_11055"
     CDS             197..658
                     /locus_tag="PKX82_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52838.1"
                     /translation="MKKLVALALAACALAPAFAQTNVGVSIGVYQPGVYGRIDIGNFP
                     QPQVIYPQPVIIHHPPVAVYQQPVYLYVPPGHQKNWRKHCSRYNACGQPVYFVQERWI
                     RDRYDERHGHDDRRDDRYDGRRDDRGDFRHDNGHGRDKHGKDDRGRHGRGD"
     gene            complement(655..1992)
                     /locus_tag="PKX82_11060"
     CDS             complement(655..1992)
                     /locus_tag="PKX82_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOF52839.1"
                     /translation="MGAKAHWHRRARHALAHSLRVRLVAMFLLLALAMTATFVFGMQY
                     ALRIGWRDAARPLVVDYVDRLAAEIGTPPDVERARAITQRLPVSVRISGPTVNWRSNP
                     GEPDYDAQREARWSSDGPRFFERTLADGHHVRFGLNVQVWHDRPRFIGWATLGILLLL
                     TAIAYARVRRMLRPLDDIRAGALRFGAGDFAQPITVKHAHRPDQLGELAATINTMGAD
                     IHQMLEAKRALLLAISHELRSPLTRARLNTELLPETPDVQPSREALLRDLALMRDLVT
                     DLLESERLSSRHAALHTEEVDLSALVADVVGSLPAGAAVQQDVSTQLPHLALDATRMR
                     LLVRNLLDNALRFTPAGGAPPVISAQPDATGGVQLVVRDFGPGVAEEQLSQLGQPFYR
                     PDTARTREGGGVGLGLYLCRLVAQAHGGTFTVRNAQPGLEVVVSLPRLAAGQG"
     gene            complement(2061..>2615)
                     /locus_tag="PKX82_11065"
     CDS             complement(2061..>2615)
                     /locus_tag="PKX82_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOF52840.1"
                     /translation="AILDVMLPEMDGFALCRAIRKDSDIPIVMLTARGEVMDRVVGLE
                     LGADDYVPKPFEPRELVARVQTVLRRRIASPAAKLVSGDGNTLVFDGLVVNATTRTVM
                     RSGETLDLTSTEFDLLLLLARAPGKVFSRDDILNQLRGHEAELYTRAVDIVVSRVRKK
                     LEPLDCIKTLRNAGYTLALVRLQA"
BASE COUNT          460 a          848 c          887 g          420 t
ORIGIN      
        1 gtccagcgcg ccccaggcgg gcaccacaca ggcggcctgg gcggagaggg cttgcacttg
       61 cggcgcccaa gcagcggcat cacacatcag gccgggcagg aggatgaggg tgggtttcat
      121 acagtttgtt gcaaagtcag gtcggccgcg gggcggtttc aaccgttaaa tatagccagt
      181 cgattggaga actgtaatga aaaaactcgt agcacttgcc ctggccgctt gcgccctggc
      241 tcctgctttt gcccagacca atgtaggcgt gtccatcggg gtgtaccagc cgggggtgta
      301 tggccgcatt gacattggca actttccgca gccccaggtg atttatccgc agccggtcat
      361 cattcaccat ccgcccgtgg cggtgtacca gcagccggtg tacctgtatg tgccacccgg
      421 ccaccagaag aactggcgca agcattgcag ccgctacaac gcctgcggtc agccggtgta
      481 ttttgtgcag gaacgctgga ttcgtgatcg ctacgacgag cgtcacggcc atgatgaccg
      541 ccgcgatgac cgctatgacg ggcgccggga cgaccgcggc gacttccgtc atgacaacgg
      601 ccacggtcgc gacaagcatg gtaaagacga cagaggccgg cacggccggg gcgattaacc
      661 ctgccctgca gccagtcgcg gcaggctgac caccacttcc agccccggtt gcgcattgcg
      721 gaccgtgaag gtgccgccat gggcctgtgc caccaggcgg cacagataca agcccagccc
      781 cacaccgccg ccctcacggg tacgggcggt gtcgggccgg tagaagggtt ggcccagctg
      841 ggacagctgc tcttctgcca caccggggcc aaagtcacgc accaccagtt gcacaccacc
      901 ggtggcgtct ggctgcgcgc tgataacggg cggcgcaccg cccgcgggcg tgaagcgcag
      961 cgcgttgtcc agcaggttgc gcaccagcag gcgcatacgg gtggcatcca gtgccaggtg
     1021 cggcagctgg gtgctgacat cctgctgcac cgcggcgccg gcgggtaggc tacccactac
     1081 gtcagctacc agggcggaca gatcgacctc ttcggtatgc agcgcggcat ggcggctgga
     1141 gaggcgttcg ctttccagca ggtcggtcac caggtcgcgc atcagggcca ggtcgcgcag
     1201 caaggcctcg cggctgggtt gcacgtcagg cgtttcaggc agcagttcgg tgttgaggcg
     1261 cgcgcgggtc agcgggctgc gcagttcatg gctgatggcc agcaataacg cgcgcttggc
     1321 ttccagcatc tggtggatgt cggcgcccat ggtgttgatg gtggccgcga gttcacccaa
     1381 ctgatcgggg cggtgcgcgt gcttgaccgt gatgggctgc gcaaagtcgc ccgcgccaaa
     1441 acgcagagcc cccgcacgga tgtcatccag cggccgcagc atgcgccgca cgcgggcata
     1501 ggctatcgcg gtgagcagca acagaatgcc cagcgtggcc cagccgatga agcgcggccg
     1561 gtcgtgccag acctgcacat tgaggccgaa gcggacatgg tgcccatcgg ccagggtgcg
     1621 ctcaaaaaag cgggggccat cggaggacca gcgcgcttcg cgctgcgcgt cgtaatcggg
     1681 ttcgccgggg ttgctgcgcc agttgaccgt ggggccgctg atgcgcacgc tcacgggcag
     1741 ccgctgggtg atggcgcgtg cacgctccac gtcgggcggg gtgccaattt cggcggcgag
     1801 cctgtcgaca tagtccacca ccagcggacg cgccgcatca cgccagccaa tgcgcagcgc
     1861 gtactgcatg ccaaacacga aggttgccgt catggccaga gccagcagca ggaacatggc
     1921 caccagccgc acgcgcagcg agtgggccag cgcgtggcgc gcgcggcgat gccagtgggc
     1981 ctttgccccc acgctccccg ctgcgcgtgg ttcgctgccc cccgaggggg ccttcgcgcc
     2041 ttggggcggc ccggcggcgc tcatgcttgc aggcgcacca gcgccagggt gtagcccgca
     2101 ttgcgcaagg tcttgatgca gtccagcggc tcgagtttct tgcgtacacg gctcaccacg
     2161 atgtcgaccg cgcgggtgta cagctcggcc tcgtggccgc gcagctggtt gaggatgtcg
     2221 tcgcggctga acaccttgcc gggggcacgg gcgagcagca ggagcaagtc aaattcggtg
     2281 ctggtgaggt ccagagtttc gccacttcgc attaccgtgc gggtggtggc gttgaccacc
     2341 aggccatcga acaccagggt gttgccatca cctgaaacca gcttggcagc aggtgaagcg
     2401 atgcgccggc gcagcacggt ctgcacccgg gccaccagtt cccgcggctc aaacggcttg
     2461 ggcacatagt catccgcccc cagctccagc ccgaccacgc ggtccatcac ctcgccacgc
     2521 gcggtgagca tgacgatggg aatgtcgctg tccttgcgaa tagcgcggca cagggcaaag
     2581 ccatccatct cgggcagcat cacatccaga atggc
//
LOCUS       DAOSJM010000348         2607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_34322_length_2607_cov_3.181034, whole genome shotgun sequence.
ACCESSION   DAOSJM010000348 DAOSJM010000000
VERSION     DAOSJM010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34322_length_2607_cov_3.181034"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="PKX82_11070"
     CDS             complement(<1..595)
                     /locus_tag="PKX82_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOF52841.1"
                     /translation="MFETAIAGSLPKPSWLSETEKLWPQWKLAGPELQQAKMDATLLW
                     IKEQEDAGLDIVGDGEQSRQHFVHGFLEQVEGIDFENKVKMGIRNNRYDAMVPQVIAP
                     LRLKGRVHAFEAQLARAHTKKKLKFTLPGPLTIVDTVADRFYGDRKAMAFAFAELLNQ
                     EALALQADGVDIIQFDEPAFNVYMQDAADWGVKALERA"
     gene            complement(613..1476)
                     /locus_tag="PKX82_11075"
     CDS             complement(613..1476)
                     /locus_tag="PKX82_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOF52842.1"
                     /translation="MAVASNDAWGKPLDGWRLRLYTIISEADTRAGRLFDQWLIAIIL
                     ASVTVVVLDSVHGINARLGTVFDILEWGFTLVFTVEYVARLLCVRHPLRYALSFYGVI
                     DLLALLPTYLALLVPEVHALIDVRVLRLLRVFRVFKLTAYMSEYQSLGRALQASRRKI
                     MVFLAAVLMIVLVMGTLMYVVEGPANGFTSIPIAVYWAVTTMTTVGFGDITPKTDLGR
                     LIASVMMLLGWGTLAVPTGIVTAEMTGQRGRETVTTRTCHECLTEGHAPEANYCLHCG
                     ARLPDYQRSTT"
     gene            complement(1490..>2607)
                     /locus_tag="PKX82_11080"
     CDS             complement(1490..>2607)
                     /locus_tag="PKX82_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF52843.1"
                     /translation="AWEAALATEGAPPALTVGPADLAVLPYTSGTTGMPKGCMHTHSS
                     IMHNAVASGLWGNGTMENVTLMVVPMFHITGMVSLMHTNMAAGATLVIMPRWDRELAG
                     QLISRYKITHWTNIPTMVIDLMASPNFGQYDLSSLVYIGGGGAAMPQAVAQRLWEQFG
                     LRYVEGYGLTETAAPSHSNPPDAPKQQCLGIPFMGVDARVVDPDTLAEMPTGGQGEIL
                     IHGPQVFKGYWKRPEATEAVFVQIDGKRFFRSGDLGHVDEDGYFFLTDRLKRMINASG
                     FKVWPAEVEALMFRHPAVQEACIISTKDAYRGESVKAVVVKRASHAQVTEAELLAWCH
                     ENMAVYKAPRSIQFVDALPKSGAGKVMWRVLQEAEGR"
BASE COUNT          473 a          848 c          807 g          479 t
ORIGIN      
        1 ccgcgcgctc cagcgccttc acgccccagt cggccgcgtc ctgcatgtag acgttgaagg
       61 ccggctcgtc gaactggatg atgtccacgc catcggcctg cagggccaga gcctcctggt
      121 tgagcagttc ggcaaaggcg aacgccatgg ccttgcggtc gccataaaaa cggtccgcca
      181 cggtatccac aatggtcagc gggccgggca gcgtgaactt gagtttcttt ttggtgtggg
      241 cgcgggccaa ctgggcctcg aacgcatgca cacggccctt gagacgcagc ggcgcgatca
      301 cctgcggcac catggcgtcg tagcggttgt tgcggatgcc catcttgacc ttgttctcaa
      361 agtcaatacc ttcgacctgc tccagaaagc catgcacaaa gtgctggcgg ctttgttcgc
      421 cgtcgcccac gatgtccagg ccggcgtctt cttgctcctt gatccacaac agcgtggcat
      481 ccatcttggc ctgttgcagc tcggggccag ccagcttcca ctgcggccag agcttttcgg
      541 tttccgagag ccaggatggc ttgggcaggc tgcctgcgat ggcggtttca aacatggtat
      601 ctcctcgttg tatcaggtcg tactgcgctg gtagtcgggc aggcgtgcac cgcagtgcag
      661 gcagtaattg gcttcgggcg cgtggccttc ggtcaggcat tcgtggcagg tgcgggtggt
      721 gacggtttcg cggccccgtt ggccggtcat ctcggcggta acgatgccgg tgggcacggc
      781 cagcgtgccc cagcccagca gcatcatgac cgaggcaatc agccgcccca gatcggtctt
      841 gggcgtgatg tcaccaaagc ccacggtggt catggtggtg acggcccagt acaccgcaat
      901 gggaatgctg gtaaagccat tggccggccc ttcgaccacg tacatgagcg tgcccatgac
      961 cagcacgatc atgagcaccg ccgccagaaa caccatgatc ttgcgccggc tggcctgcag
     1021 cgcccgcccc agcgactggt actcggacat ataggccgtg agcttgaaca cccggaacac
     1081 gcgcagcagg cgcaacaccc gcacatcaat cagcgcatgc acctcgggca ccagcaaggc
     1141 cagataggtg ggcaacagcg caagcaaatc gatcacacca taaaagctca gggcatagcg
     1201 caacggatgg cgcacgcaca gcaggcgcgc cacatactcc acggtaaaca ccagggtgaa
     1261 gccccactcc agaatgtcga acaccgtgcc caaccgcgcg ttgatgccgt gcacgctgtc
     1321 aagcaccacc accgtgacac tggcgaggat gatggcaatc agccactggt caaacagcct
     1381 gcctgcgcgc gtgtccgcct cagaaataat ggtgtaaagc cggagccgcc agccgtccag
     1441 cggcttgccc catgcgtcgt tcgatgccac tgccattagc gtcctcttgt tatcgtcctt
     1501 ctgcttcctg caagacccgc cacatgactt tacccgcccc gctcttgggc agggcatcga
     1561 caaactggat ggagcgcggg gccttgtaga ccgccatatt ctcatggcac caggccagca
     1621 gctcggcctc ggtcacctgc gcgtggctgg cgcgcttgac gaccaccgcc ttgaccgact
     1681 cgccgcggta ggcgtctttg gtggagatga tgcaggcttc ctgcactgca gggtggcgga
     1741 acatcagcgc ttccacctcg gcgggccaga ccttgaagcc cgaggcattg atcatgcgct
     1801 tgagccggtc ggtcaggaaa aaatagccgt cttcgtccac atggcccagg tcgcccgacc
     1861 gaaagaagcg cttgccgtcg atctgcacga atacggcctc ggtggcctcg ggccgcttcc
     1921 agtagccctt gaacacttgc gggccgtgga tcagaatttc gccctgcccg ccggtgggca
     1981 tttccgccag cgtgtccggg tccaccacgc gcgcgtccac ccccatgaac ggaatgccca
     2041 ggcactgctg cttaggcgca tcgggcgggt tgctgtgcga gggggctgcg gtttcggtca
     2101 ggccatagcc ctccacatag cgcaggccaa actgctccca caggcgctgg gccacggcct
     2161 gtggcatggc cgcaccgcca ccgccgatgt agaccaggct ggagaggtcg tactgcccga
     2221 agttggggct ggccataagg tcaatcacca tggtggggat gttggtccag tgcgtgatct
     2281 tgtagcggga aatcagttgc cccgccagct cgcggtccca gcgcggcatg atgaccagcg
     2341 tggcccctgc ggccatgttg gtgtgcatca gactcaccat gccggtgatg tggaacatgg
     2401 gcaccaccat cagcgtgacg ttttccatgg tgccattgcc ccacaggccg ctggccaccg
     2461 cgttgtgcat gatgctgctg tgcgtgtgca tgcagccctt gggcatgccg gtggtgccgc
     2521 tggtgtaagg cagcacagcc agatcagcgg gccccacggt cagcgccggc ggcgcgcctt
     2581 cggtggccag cgcagcctcc caggcgt
//
LOCUS       DAOSJM010000349         2602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_34409_length_2602_cov_2.437770, whole genome shotgun sequence.
ACCESSION   DAOSJM010000349 DAOSJM010000000
VERSION     DAOSJM010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34409_length_2602_cov_2.437770"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PKX82_11085"
     CDS             complement(<1..663)
                     /locus_tag="PKX82_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF52844.1"
                     /translation="MTGSLAATPAWVPSPVVEIRGLCTVFRTDGVDAQIHKNLDLHIE
                     RGELLSIVGGSGSGKTTLLRLMLGLESPMGGSVDVLGRPAVELTTSGAASRVGMLFQQ
                     GALYSAFTVLENIAFPLRELKSLPDDLVRDAVMVKLQMVGLDPAHAHKMPADLSGGMI
                     KRVALARALIMDPPLLLLDEPTAGLDPDRSDAFCTLLRSLHQELGLTVVMVTHDLDTI
                     YEI"
     gene            complement(660..1802)
                     /locus_tag="PKX82_11090"
     CDS             complement(660..1802)
                     /locus_tag="PKX82_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF52845.1"
                     /translation="MSLAAREESRPRIDIGHLPEGVCVRLLGRWTAGNLANRDNWQAL
                     QNSLCALPQDWRTCHWDLRELQRMDHVGAQLLWNAWYRQWPDTVHTLAAQRALLERIA
                     QFSSVLPPRRPQTLWDWVLRGGALVIRVQGHLVDAVGLIGQLLLDMAQLVRAPRNGPW
                     RDISGNLYRIGATALPITALVGFLIGVVLAYLMAQQLRQFGADAFIVDILGISLIREL
                     GPVLAAILIAGRSGSAITAQIGVMRVTEELDAMKVMGIPQGFRLVLPRAIAMAVAMPL
                     LSVWTTLAALSGGVLAADVVLGLTPGYFVTALAKVVLVSNLWLAVGKSVVFGLLIALI
                     GCHYGLRIKPNTQSLGEGTTASVVTSITMVLLVDALFAVVFKDIGI"
     gene            1899..>2602
                     /locus_tag="PKX82_11095"
     CDS             1899..>2602
                     /locus_tag="PKX82_11095"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52846.1"
                     /translation="MTTHATLSRRAPRRFWGWGQADATLDAREQATVKTMVEQLDVPY
                     AERPVPQVHEFTLPAPRVMPPPALAASFSDKPLDRLNHCVGKSYADLARMWLRSAPTP
                     PDRVAYPDDEQAVVDILDWAQTHNVAVIPYGGGSSVCGGVEAAVGDSYAGVISLDLER
                     LNRVLEVDTASRAARIQAGALGPELQAQLKPHGLTLRHFPQSFEFSTLGGWIVTRAGG
                     HYATQTTHIDDLVESTR"
BASE COUNT          501 a          892 c          816 g          393 t
ORIGIN      
        1 gatctcgtaa atcgtgtcca ggtcatgggt gaccatcacc accgtcaagc ccagctcctg
       61 gtgcagcgag cgcagcaggg tgcaaaacgc gtctgagcgg tccgggtcca gcccggcggt
      121 gggttcgtcc agcaagagca gcggcgggtc catgatgagc gcgcgcgcca gcgccacgcg
      181 cttgatcatg ccgcccgaca aatccgccgg catcttgtgg gcgtgggccg gatccagccc
      241 caccatctgt agcttgacca tgaccgcatc gcgcaccagg tcgtcgggca gggatttgag
      301 ttcgcgcagt ggaaacgcaa tgttctccag cacggtaaac gccgagtaca gcgcgccctg
      361 ctgaaacagc atgcccacgc ggctggccgc gcccgaggtg gtgagctcca ccgccggtcg
      421 ccccagcacg tcgacactgc cacccatggg cgactccagc cccaacatca ggcgcagtag
      481 cgtggtcttg ccgctgcccg agccaccgac gatggacagc aactcgccgc gctcgatgtg
      541 cagatccagg ttcttgtgga tctgcgcgtc cactccgtcg gtgcgaaaca cggtgcacag
      601 gccacggatt tccaccacag gagacggcac ccaggcgggg gtagctgcca atgagccggt
      661 catatgccaa tgtccttgaa aaccactgca aacaaggcgt ccaccagcag caccatggtg
      721 atgctggtca ccaccgaagc cgtggtgcct tcgcccaggc tctgcgtgtt gggcttgatg
      781 cgcagcccgt agtggcagcc gatgagcgca atcagcaatc caaacaccac cgacttgccc
      841 accgccagcc acaggttgga gaccagcacc accttggcca atgcagtgac gaaatagccg
      901 ggagtcagac ccaacaccac gtcagccgcg aggacaccgc ctgacagcgc agccagcgtg
      961 gtccacaccg acagcagcgg catggccacc gccatggcga tggcgcgcgg cagcaccagc
     1021 ctgaaaccct gcgggatgcc catgaccttc atggcatcga gctcttcggt gacccgcatc
     1081 acgccaatct gcgcggtgat agccgagccc gaacgccccg caatcaggat ggcggccagc
     1141 accggcccca attcacgaat gagcgatatg cccaggatgt caacaatgaa cgcgtccgct
     1201 ccaaactggc gcaactgctg cgccatcaga taggccagca ccacgccgat caagaagccc
     1261 accaacgcag tgatgggcag ggcggtagcg cctatgcggt agaggttgcc agagatgtcg
     1321 cgccacggcc cgttgcgcgg tgcgcgcacc agttgcgcca tgtccagcag caattggccg
     1381 atcagcccaa cggcgtccac caggtgccct tggacccgaa tgaccagtgc ccccccacgc
     1441 agcacccagt cccacagggt ctggggtctg cgcggcggca agacggaaga aaactgcgcg
     1501 atgcgctcca gcagcgcgcg ttgcgcggcc agggtgtgca cggtatcggg ccactgccgg
     1561 taccaggcat tccacagcag ctgggcgccc acatggtcca tgcgctgcag ttcgcgcaag
     1621 tcccagtggc aggtgcgcca gtcctgcggc aacgcgcaca ggctgttctg caaggcctgc
     1681 cagttgtccc ggttggccag atttcccgcc gtccagcgcc ccagcagccg cacgcacacg
     1741 ccttcgggca gatgcccgat gtcaatgcgg ggacgggatt cctcccgggc ggccagcgac
     1801 atggtgggtt ggcagtggta gcggcggtat tgacatggta caggctggac ggtccgggta
     1861 gaatagcgat ttgccccata ccagcccagg attcgcacat gaccacccac gccacacttt
     1921 cccgccgcgc cccgcgccgt ttctggggtt ggggccaggc ggatgccacg ctggatgcac
     1981 gcgaacaggc cacggtgaag accatggtcg aacagctcga cgtgccctat gccgagcgac
     2041 ccgtacccca ggtgcatgaa ttcacactgc ccgcgccgcg cgtgatgccg ccacccgcgc
     2101 tggcagccag tttttcagac aagccgctgg accggctcaa ccactgcgtg ggcaagagct
     2161 acgccgacct ggcgcgcatg tggctgcgca gcgcacccac tccgccggat cgggtggcct
     2221 acccggatga cgagcaggcg gtggtagaca tacttgactg ggcgcaaacc cacaatgtgg
     2281 ccgtcattcc ctatggcggc ggcagcagcg tgtgcggcgg cgtggaagcc gccgtgggcg
     2341 acagctacgc cggtgtgatt tcgctggacc tggagcgtct gaaccgcgtg ctggaagtgg
     2401 acactgcgag ccgggccgct cgcatccagg ccggtgcgct ggggccggag ttgcaagccc
     2461 agctcaagcc ccatggcctc acgctgcgcc actttccgca gagctttgag ttctccacgc
     2521 tgggcggctg gatcgtcaca cgggccggcg gtcactacgc cacgcagacc acgcacatcg
     2581 atgacctggt ggagtccacg cg
//
LOCUS       DAOSJM010000350         2599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_34450_length_2599_cov_4.711871, whole genome shotgun sequence.
ACCESSION   DAOSJM010000350 DAOSJM010000000
VERSION     DAOSJM010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34450_length_2599_cov_4.711871"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKX82_11100"
     CDS             complement(<1..406)
                     /locus_tag="PKX82_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOF52847.1"
                     /translation="MKKSKLVMIGNGMAGVRTLEELLKIAPDLYDITVFGAEPHPNYN
                     RILLSPVLAGEQTLDEIVLNDWSWYTDNHITLHAGWKVTEVDRVRRVVHAENTAGEKM
                     SAEYDRLLVATGSNPFILPIPGKDLEGVIAYRD"
     gene            730..1989
                     /locus_tag="PKX82_11105"
     CDS             730..1989
                     /locus_tag="PKX82_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HOF52848.1"
                     /translation="MTSALLCIHRPQTAPTLAADLSSAGISVLATLEDCSKLVQAVVL
                     HAPDVVICDVPLPTSAWFQAWQTLEQTLPCPTLVFTQDADATHIAQAVECGVHVYVVH
                     GYGANRLRSLIHLAQARHQKAQQQRQAFDAMATRFEERKAVDRAKGILMRAQSLSDDD
                     AFRTLRTAAMNSNQRIGQLSQHIIQSAHFAEGLNRAGQLRMLSQRLVKLHLLQAAGVQ
                     AAHHAALLQESVQWVDSNFALLRKNLSMPTYGDLLEQVAHTWEQLKTALATGQTSAVQ
                     DSAEALLLGAERLTSSLEGSGAAAPLQVLNLAGRQRMLSQRFAKFALLAAEADMRTVQ
                     QEFEAALTYLNGLPLSTPDIQAALAAAGVAWLQMVAAAKEAQRLPAAQRSARLEELAT
                     GSETLLGLFEQLSVHYERSMQMLMGEA"
     gene            2061..2546
                     /locus_tag="PKX82_11110"
                     /pseudo
     CDS             2061..2546
                     /locus_tag="PKX82_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
BASE COUNT          461 a          831 c          836 g          471 t
ORIGIN      
        1 tgtcgcggta ggcaatcacg ccctccagat ccttgccggg aatcggcagg atgaacgggt
       61 tggagccggt ggccaccagc aggcggtcgt attcggcgct catcttttcg ccggctgtgt
      121 tctcggcatg caccacgcgg cgcacgcggt ccacctcggt cactttccag cccgcgtgca
      181 gggtgatgtg gttgtcggtg taccaggacc agtcgttgag cacgatctcg tccagcgtct
      241 gctcaccagc cagcaccggg ctcagcagga tgcggttgta gttggggtgg ggttctgcac
      301 caaaaacggt gatgtcgtac agatcgggtg cgattttcaa cagctcttcg agcgtgcgca
      361 cgccggccat gccgttgcca atcattacca gtttcgattt tttcatagtg gccactccag
      421 aaaacaagaa gtcattgcgg ctgcagcacg gctgcacgcc agaaaaatag gggtagagga
      481 gatccgcgcc ttatgcaaag gcgcaccaga acacactggt gtgggacaga ctgcaactgg
      541 gcgacgtcgt tgtgcgcaga atgggtcagg ctgtcatctc gttcggacgc gtcgttgcgt
      601 cctgtgggta cttatgcaaa agctgtgcca tatgattgcc acctcgtgtt caggccctgc
      661 ccatccgggt gcctggtgtg cgacttgcat ggctgcaaag cctgttctcc ctagctacca
      721 ctgattttta tgacctccgc cctgctgtgt atccaccgtc cccaaaccgc ccccacactg
      781 gcggcggacc tgtcatcggc gggtatttcg gtgctggcca cgctggaaga ttgcagcaag
      841 ttggtgcagg cggtggtgct gcacgcaccc gatgtggtga tttgcgatgt gcccctgccc
      901 accagcgcat ggttccaggc atggcaaacg ctggagcaga cgctgccttg ccccaccctc
      961 gtgttcaccc aggatgccga tgccacccat attgcccagg ctgtggagtg cggggtgcat
     1021 gtgtatgtgg tgcacggcta tggcgccaac cgcctgcgtt cactcatcca cctggcccag
     1081 gcgcgccacc agaaggcaca gcagcagcgg caggcctttg acgccatggc cacccgcttt
     1141 gaagagcgca aggcggtcga ccgggccaag ggcatcctga tgcgggccca gagcctgtcg
     1201 gacgacgacg ccttccgcac cctgcgcacg gccgccatga actccaacca gcgcattggg
     1261 cagctgtcgc agcacatcat ccagtcggcc cattttgcgg aagggctgaa ccgcgctggc
     1321 cagttgcgca tgctctcgca gcggctggtc aagctgcacc tgctgcaggc ggccggggtg
     1381 caggccgccc accacgccgc gctgctgcag gagtcggtgc aatgggtgga cagcaacttt
     1441 gcgctgctgc gcaaaaacct ctccatgccc acctatggcg atctgctgga gcaggtggcg
     1501 cacacctggg agcagctcaa gacggcattg gccaccggcc aaaccagtgc ggtgcaagac
     1561 agtgccgagg ccctgctact gggcgccgag cgcctgacca gcagcctgga gggctcgggc
     1621 gcagcggccc ccctgcaggt gctgaacctg gccgggcgcc agcgcatgct gtcgcagcgc
     1681 ttcgcgaaat ttgcgctgct ggccgccgag gcggacatgc gcaccgtgca gcaggagttt
     1741 gaagcggcgc tcacctacct caacggcctt ccgctgagca cgcccgacat ccaggccgct
     1801 ctggctgcgg cgggcgtggc ctggctgcaa atggtggccg cggccaaaga ggcgcagcgc
     1861 ctgccggcgg cccagcgcag tgcgcggctg gaggagctgg caacgggcag cgaaacactg
     1921 ctgggcctgt tcgagcagct ctcggttcac tacgagcgca gcatgcaaat gctgatgggc
     1981 gaagcgtgag ccaagggtta acccgcacaa ctttggtgca gtgcagtagc gctgggcact
     2041 gatattgctt atcttggtgc atggcttttg acatagacat tggctttgca tcccaggctg
     2101 ggcgcaaacc gcttaacgaa gacttttgtg ccgccatgct gcccacacca gggcaggaag
     2161 acatgggctc catcgtggcc attgccgatg gcgtgagtgc cggcggcatg gggcgcgaag
     2221 ccgcgcagac cacggccacc agcgtggtgc gcgactactt tggcacgccc gagacctggg
     2281 acaccaccgt ggcgctggac cgcatcatcg ctgcgcagaa tgcctggctg tgtggcatca
     2341 accggcgccg ccagcccgcc atgggcctga ctacgctcac cgccctggtg ctgcgcggcc
     2401 agtcgtacac gctggcgcat gtgggcgaca cccgctgcta cctggtgcgc aatggccagg
     2461 ccacgcagct cacgcaggac catgtggtca accacccgga cttcaagcac cagctgctgc
     2521 gctcgggtgg cagcccagaa cggctcggcc gccggcgatg atcgcgactt cgtcgcagag
     2581 gcgttcggca tgggcgatg
//
LOCUS       DAOSJM010000351         2595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_34513_length_2595_cov_5.756299, whole genome shotgun sequence.
ACCESSION   DAOSJM010000351 DAOSJM010000000
VERSION     DAOSJM010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34513_length_2595_cov_5.756299"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /gene="rpsA"
                     /locus_tag="PKX82_11115"
     CDS             <1..1352
                     /gene="rpsA"
                     /locus_tag="PKX82_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOF52849.1"
                     /translation="TTSGKVKGGLTVLVNGIRAFLPGSLVDTRPTKDLSPYENKTLEF
                     KVIKLDRKRNNVVLSRRAVVEASMGEERAKLMHTLKEGSVVQGVVKNITEYGAFVDLG
                     GIDGLLHITDMAWRRVRHPSEVVTAGQEITAKILKFDTEKNRVSLGLKQMGDDPWMGV
                     NRRYPQGTRMFGKITNIADYGAFVELEPGIEGLVHVSEMDWTNKNVAPSKIVALGDEV
                     EVMVLEIDEDKRRISLGMKQCKANPWQEFAQNTKRGDRVKGPIKSITDFGVFVGLAAG
                     IDGLVHLSDLSWNEPGETAVREYKKGQEVEALVLAVDVDRERISLGIKQLDSDPFTTF
                     VSINDKGQIVTGKVKTVDAKGAEIDLGEDIIGYLRASEISRDRVEDARNVLKEGDEVT
                     AVVVNVDRKTRNIQLSIKAKDMADQNEAMQTLSQQSARENAGTTSLGALLRAKLDNN"
     gene            1440..1751
                     /locus_tag="PKX82_11120"
     CDS             1440..1751
                     /locus_tag="PKX82_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HOF52850.1"
                     /translation="MTRSDLVEELANRFSQLTHRDAEFAVKAILDAMNDALVRGHRIE
                     IRGFGSFSINRRPPRMGRNPRSGESVAIPEKRVPHFKPGKALREAVDQRTAELESKAK
                     P"
     gene            1879..2196
                     /locus_tag="PKX82_11125"
     CDS             1879..2196
                     /locus_tag="PKX82_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOF52851.1"
                     /translation="MKQIAWLFKWLLKAAIFFTLFAFALNNQGDTTVHFFFGYRWTAP
                     MVLVVLGAFASGLVIGVLGMVPRWWRHRRDAVQARLAASQAAIALSAEASKQTPAPRP
                     DGI"
     gene            2186..>2595
                     /locus_tag="PKX82_11130"
     CDS             2186..>2595
                     /locus_tag="PKX82_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HOF52852.1"
                     /translation="MEFDITLILLGLPVAFVLGWVASRMDMRQLRMENRQAPKAYFKG
                     LNFLLNEQQDQAIDAFIEAVQNDPDTSELHFALGNLFRRRGEYERAVRVHQHLLDRGD
                     LSLDDRHRAQHALALDYLKAGLLDRAEDALLKLEG"
BASE COUNT          554 a          814 c          750 g          477 t
ORIGIN      
        1 gcaccacttc cggcaaggtc aagggcggcc tgaccgttct ggtcaacggc atccgcgcat
       61 tcctgcccgg ctcgctggtc gacacacgtc ctaccaagga cctgtctccc tacgagaaca
      121 agaccctgga attcaaggtt atcaagctgg atcgcaagcg caacaatgtg gtgctgagcc
      181 gccgcgctgt ggtggaagcc tccatgggcg aagagcgcgc caagctgatg cacaccctca
      241 aagaaggttc tgtggtgcaa ggcgtggtca agaacatcac cgaatacggt gcgttcgtgg
      301 acctgggcgg tatcgacggc ctgctgcaca tcaccgacat ggcatggcgc cgtgtccgtc
      361 acccttccga ggtcgtgact gctggtcaag agatcactgc caagatcctg aagttcgaca
      421 ccgaaaagaa ccgcgtgtct ctgggtctga agcaaatggg cgacgatccc tggatgggcg
      481 tgaaccgccg ctacccccaa ggtacccgca tgttcggcaa gatcaccaac atcgccgact
      541 acggcgcgtt tgtggaactc gaacccggta tcgaaggtct ggtgcacgtg tccgaaatgg
      601 actggaccaa caagaacgtt gctccttcca agatcgttgc cctgggtgac gaagtcgaag
      661 tcatggttct ggaaatcgac gaagacaagc gtcgcatctc cctgggcatg aagcagtgca
      721 aggccaaccc atggcaagag tttgctcaaa acaccaagcg cggcgaccgc gtgaagggcc
      781 cgatcaaatc catcaccgac ttcggcgtgt tcgtcggtct ggctgctggt atcgacggcc
      841 tggtgcacct gtctgacctg tcctggaacg agcccggcga aaccgctgtg cgcgaataca
      901 agaagggcca ggaagttgaa gctctggttc tggctgtgga cgtggaccgc gaacgcatct
      961 ccctgggtat caagcagttg gattccgatc ctttcaccac cttcgtgtcg atcaacgaca
     1021 agggccagat cgtgaccggc aaggtcaaga ccgtggatgc caagggcgct gaaatcgacc
     1081 tgggtgaaga catcattggt tacctgcgcg cttccgaaat cagccgcgac cgcgttgaag
     1141 acgctcgcaa cgtactgaaa gaaggcgacg aagtgactgc tgtggttgtc aatgtggatc
     1201 gcaagacccg caacatccag ttgtccatca aggccaagga catggctgac cagaacgaag
     1261 ccatgcaaac cctgagccag cagtctgccc gtgaaaacgc tggtaccacc agcctgggcg
     1321 ccctgctgcg cgccaagctc gacaacaact aagccctgtt gttgatctga tcaaacgacc
     1381 ggccacccca agtgatcttt gggtgccggt cgttttttta ttgaataccc acttccagca
     1441 tgacccgctc cgacctcgtt gaagaactgg ccaaccgttt cagccagctg acccatcgcg
     1501 atgccgaatt cgccgtcaag gccattctgg atgccatgaa cgacgccctg gtgcgcggac
     1561 accgcattga aatccgtggt tttggcagct tctccatcaa ccgccggccg ccccgcatgg
     1621 gtcgcaatcc ccgcagcggt gagagcgtgg ccatccccga gaagcgtgtc ccccacttca
     1681 agcccggcaa ggccctgcgc gaagccgtgg accaacgtac tgccgaactg gagtccaagg
     1741 ccaaacccta aaacgcggag attgagcacc gccgggccgc cccaaggcgc gaaggccccc
     1801 gcggggggca gcgacccgtg caacggtgga gcgtgggggc actactcatt tagaatcact
     1861 cccacaccga ggagatgaat gaaacagatc gcatggctct ttaagtggtt acttaaagcg
     1921 gccatttttt ttaccctgtt cgctttcgcg ctgaacaacc agggcgacac gacggtgcac
     1981 tttttctttg gctaccgctg gaccgctccc atggtgctgg tcgtgctggg tgccttcgca
     2041 tccggcctcg tcattggtgt actgggcatg gtgccgcgct ggtggcgcca ccgccgtgat
     2101 gccgtgcagg cgcgtctggc cgcaagccag gctgccattg ccctgagcgc agaagccagc
     2161 aaacaaaccc cggcaccccg acccgatgga atttgatatc accctgatcc tgctgggcct
     2221 gcccgtggcc ttcgtgctgg gctgggttgc atcccgcatg gatatgcgcc agttgcgcat
     2281 ggaaaaccgc caggctccca aagcctattt caaaggcctg aacttcctgc tcaacgagca
     2341 gcaggaccaa gccatcgacg cctttatcga agccgtccag aacgaccccg acacctcgga
     2401 actccacttc gcgctgggca acctgtttcg ccgccgcggc gaatacgagc gggccgtgcg
     2461 cgtgcaccag cacctgctgg accggggcga cctgagtctg gacgaccgcc accgcgccca
     2521 gcatgcgctg gcgctggact acctcaaggc cggcctgctg gaccgcgcgg aagatgcctt
     2581 gctcaagctg gaagg
//
LOCUS       DAOSJM010000352         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_34572_length_2592_cov_4.250296, whole genome shotgun sequence.
ACCESSION   DAOSJM010000352 DAOSJM010000000
VERSION     DAOSJM010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34572_length_2592_cov_4.250296"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..559
                     /gene="nusB"
                     /locus_tag="PKX82_11135"
     CDS             23..559
                     /gene="nusB"
                     /locus_tag="PKX82_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOF52853.1"
                     /translation="MSEYNHPSHPKRPPPQPRKGLTSTGARKAASKNNRSRAREFALQ
                     GLYQSLVGGNSVSDIDPFTRDLAGFNKADAVHFDALLHGCVEQALALDALITPLLDRP
                     MAEISPVEHAVMWIGTYEMQNCPDVPWRVVINECVELAKEFGGTDGHKYVNGVLNGLA
                     PTLRALEVEADRTKKPRA"
     gene            573..1757
                     /locus_tag="PKX82_11140"
     CDS             573..1757
                     /locus_tag="PKX82_11140"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOF52854.1"
                     /translation="MQIASRAEKIEPFYVMEVAKAASELGRAVADTDAPMVFLNIGEP
                     DFTAPPLVQEAAVHAVHQGHTQYTQATGLGPLRERISDWYASRFGAQVPARRIVITAG
                     ASAALHLACMALIEAGDEILMPDPSYPCNRHFVSAAEGKAVLIPTTATERFQLSADKV
                     QAAWGERTRGVLLASPSNPTGTSIHPDELRRIHQVVQSRGGITMVDEIYLPLSYDAQF
                     GQTALALDEHIISINSFSKYFNMTGWRLGWMVVPDALVPVIERLAQNLFICPSTIAQH
                     AALACFEPESIPLYEQRRTEFQARRDYFIPELNAMGLTVPVMPDGAFYAWADCSAACQ
                     KLGVKDSWDFAYEVMNRAHVAVTPGRDFGTAQTANFVRFSTASSLEQLQIAVARLRTL
                     LL"
     gene            1789..2481
                     /gene="tolQ"
                     /locus_tag="PKX82_11145"
     CDS             1789..2481
                     /gene="tolQ"
                     /locus_tag="PKX82_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOF52855.1"
                     /translation="MNQDLSILHLVLNASIVVQAVMLLLMLVSIASWAAIFRKLFSLK
                     KVKAQNEAFERDFWSGSSLNDLFAAATQNARNCGPMERIFASGMREFQKLRERRITDA
                     GTLMDGARRAMRASFQREMDVVETNLSFLASVGSVSPYVGLFGTVWGIMHAFTGLAAL
                     TQVTLATVAPGIAEALVATAIGLFAAIPAVVAYNRFARDLDRIAIQQETFIEEFSNIL
                     QRNVGTQAPASR"
     gene            2508..>2592
                     /locus_tag="PKX82_11150"
     CDS             2508..>2592
                     /locus_tag="PKX82_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HOF52856.1"
                     /translation="MPAVTGRGGRGRRTINEINMVPFIDVML"
BASE COUNT          507 a          867 c          755 g          463 t
ORIGIN      
        1 ccaacctgct ggagagcatg taatgagtga atacaaccat cccagccacc ccaagcgccc
       61 gccgccccag ccccgcaagg gcctgaccag cacaggcgcg cgcaaggccg cgagcaaaaa
      121 caaccgcagc cgcgcacgcg agttcgcgtt acaggggctc taccagagcc tggtcggggg
      181 caactccgtg agcgatatcg atccgttcac ccgcgacctg gctggcttca acaaggccga
      241 cgcggtgcat ttcgatgccc tgctgcacgg ctgcgtggag caggccctgg cactcgacgc
      301 cctgatcacg cccttgctgg accgccccat ggcggagatt tcgccggtcg aacatgcagt
      361 gatgtggatt ggcacctacg aaatgcagaa ctgccccgac gtcccatggc gggtggtcat
      421 caacgaatgc gttgagctgg ccaaagagtt cggtggcacc gatggccaca agtacgtcaa
      481 cggcgtactc aacggcttgg caccaaccct gcgcgcactg gaagtggaag ccgaccgcac
      541 caagaagccc cgcgcctgag tcggacagca ccatgcagat tgcgtcgcgc gcggaaaaga
      601 tcgaaccctt ttatgtgatg gaagtggcca aggccgcctc cgaattgggc cgtgcggtgg
      661 ccgacaccga tgcgcccatg gtgttcctga acattggcga gccggacttc accgccccac
      721 cgctggtgca agaagccgca gtccatgcgg tacatcaagg gcacacccaa tacacccagg
      781 ccaccggcct cggcccgctg cgcgagcgca tcagcgactg gtacgccagc cgcttcggcg
      841 cgcaggtgcc tgcccggcgc atcgtgataa cggccggcgc ttcggcggcc ctgcatctgg
      901 cctgtatggc cctgatcgaa gccggggatg agatcctcat gccggacccc agctatccct
      961 gcaaccggca ttttgtgagt gctgcggaag gcaaggcggt actgattccc accaccgcaa
     1021 ccgaacgctt ccagctcagc gccgacaaag tgcaagccgc ttggggcgaa cgtacgcggg
     1081 gcgtgctgct cgcatcgcct tccaacccca cgggtacatc cattcacccg gacgaattac
     1141 gccgcatcca ccaggtagtg cagtcgcgcg gtggcatcac gatggtggac gagatttacc
     1201 tgcccttgag ctacgacgcg cagtttggcc aaaccgccct ggcgctggat gagcacatca
     1261 tcagcatcaa cagcttcagc aaatatttca acatgaccgg ctggcgcctg ggatggatgg
     1321 tggtacctga tgcactggta ccggtgatcg agcgcctggc gcaaaacctg ttcatctgcc
     1381 ccagcaccat tgcccagcat gccgcgctgg cctgcttcga acccgaaagc attccgctgt
     1441 acgagcagcg tcgcaccgaa ttccaggcgc ggcgcgacta cttcatcccc gagctcaacg
     1501 ccatgggcct gaccgttccg gtcatgcccg acggcgcttt ttacgcatgg gccgactgct
     1561 ctgcggcttg tcagaagctg ggtgtgaagg acagctggga ttttgcctac gaagtcatga
     1621 accgcgccca cgtggcggtc acaccggggc gggattttgg cactgcacaa accgcgaact
     1681 ttgtgcgctt ttccaccgcc agttctttag aacagttaca aattgccgtg gcgcgtttgc
     1741 gcacactgct gctgtaatac attgcattga ctcacccaac cggacaccat gaaccaggac
     1801 ctttccattc tccatctggt actcaacgcc agcatcgtgg tgcaagccgt catgctgctg
     1861 ctgatgctgg tctccattgc cagctgggca gctatcttcc gcaagctgtt ctcactcaaa
     1921 aaggtcaagg cccagaacga ggcgttcgag cgcgacttct ggtccggctc cagcctcaac
     1981 gacctgtttg ccgcagccac ccagaacgcc cgcaactgcg gccccatgga acgcatcttc
     2041 gccagcggca tgcgcgaatt ccagaaactg cgtgaacgcc gcatcaccga tgccggcacc
     2101 ctgatggacg gcgcgcgccg tgccatgcgc gccagcttcc agcgtgagat ggacgtggtg
     2161 gaaaccaatc tgtcgttcct ggcctcggtg ggctcggtct ccccgtatgt gggcctgttt
     2221 ggcacggtgt ggggcatcat gcacgccttc accgggctgg ctgccctgac ccaggtgacg
     2281 ctggccacgg tggctcccgg cattgcagag gccctggtcg ccacggccat tggcctgttc
     2341 gctgccatcc cggcggtggt ggcctacaac cgctttgccc gcgacctgga tcgcattgcc
     2401 atccagcagg agaccttcat tgaagagttc tccaacatcc tgcagcgcaa tgtgggcacg
     2461 caggctccag ccagccgcta gcccccgtcc cacaaggaga tagcgcgatg cccgcagtaa
     2521 caggacgtgg tggccggggc cgccgcacca tcaacgagat caacatggtc ccgttcatcg
     2581 acgtgatgct gg
//
LOCUS       DAOSJM010000353         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_35067_length_2563_cov_6.611244, whole genome shotgun sequence.
ACCESSION   DAOSJM010000353 DAOSJM010000000
VERSION     DAOSJM010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35067_length_2563_cov_6.611244"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="PKX82_11155"
     CDS             <1..1511
                     /locus_tag="PKX82_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOF52857.1"
                     /translation="LTGVMYVLDEPSIGLHQRDNDRLIGTLQHLRDIGNSVLVVEHDE
                     DMMRAADHVIDMGLGAGVHGGRVIAQGTFDDVKANPESLTGQYLAHTLQIEVPKRRTP
                     WLPVVAEANPVAKGSGKGAPSKAALAWAERQAHHIATQGDLQAIRITGASGHNLKDVS
                     VDFPVGLLTCVTGVSGSGKSTLVNDTLYAAVARQLYRAHDEPAAHESIDGIQYFDKVI
                     NVDQSPIGRTPRSNPATYTGLFTPIRELMAEVPVARERGYGPGRFSFNVAGGRCEACQ
                     GDGMVKVEMHFLPDVYVPCDVCAGQRYNRETLEVLYKGKNIAQILDMTVEAAYAFLQA
                     VPTIARKLQTLLDVGLSYIRLGQAATTLSGGEAQRVKLALELSKRDTGRTLYILDEPT
                     TGLHFADIALLLKVLHQLRDAGNTIVVIEHNLDVIKTADWLIDMGPEGGSGGGTVVGV
                     GTPEDIAANPASHTGRYLKPILEIQVPNWHLAPVEYAQAAIKSVAVGVVTPP"
     gene            complement(1498..>2563)
                     /locus_tag="PKX82_11160"
     CDS             complement(1498..>2563)
                     /locus_tag="PKX82_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HOF52858.1"
                     /translation="TRAQYHSSNRTLYPLPPQLYSHNDQQSIWQSSRPEGSKIGWGGN
                     MGDLVVPPNPSLNSNSLFTGISVSGNAVFLSGDTALQYQVSTGGAIKIRPATDTAVYG
                     SSVVRSTLNSLIQQTTGTQKLENDYNTVTKRALDSEAAITSALALSAGASAPPWVAAF
                     DTTNNNLAQQLKMVARLIHGRTQLGNRRQVFFVSIGGFDLHDGLIAQHPTILGRVSSA
                     MTAFYNATDEMGIANQVTTFTASDFGRTLASNGDGSDHGWGSHHFVVGGAVKGKAFYG
                     SPPPVSISDTKDSGGNYLPENQWHVGQGRLLPTTSVDQYAATMAKWFGVNPGAEMATV
                     LPNIGNFGGAYPVDLGFMAA"
BASE COUNT          502 a          830 c          794 g          437 t
ORIGIN      
        1 gcctgaccgg cgtgatgtac gtgctggacg agcccagcat cggcctgcac cagcgcgaca
       61 acgaccgcct gatcggcacg ctgcagcacc tgcgcgacat tggcaacagt gtgctggtgg
      121 tggagcacga cgaagacatg atgcgcgccg ccgaccatgt gatcgacatg ggcctgggcg
      181 cgggcgtaca cggcgggcgc gtgatcgccc agggcacgtt tgacgacgtc aaggccaacc
      241 ccgaatcgtt gaccggccag tatctggcac acacgctgca aatcgaagtg cccaagcgcc
      301 gcacaccctg gttgcccgtg gtagcagagg ccaaccccgt ggccaaaggt agcggcaaag
      361 gcgcccccag caaggccgcg ctggcctggg ccgaacgcca ggcgcaccac atcgccaccc
      421 agggggacct gcaagccatc cgcatcaccg gtgcatcggg ccacaacctc aaggacgtga
      481 gcgtggactt ccccgtgggc ctgctgacct gcgtgaccgg cgtctccggc tctggaaaat
      541 ccaccctggt caacgacacg ctgtacgccg ccgtggcgcg ccagctctac cgcgcgcacg
      601 acgagcccgc ggcacacgag agcatcgatg gcatccagta cttcgacaaa gtcatcaacg
      661 tcgatcagtc gcccattggc cgtacgccgc gctccaaccc cgccacctac accggcttgt
      721 tcacccccat ccgcgagctg atggccgaag tgccggtggc gcgcgaacgc ggctacggcc
      781 cgggccgctt cagcttcaac gtggccggcg gccgctgcga ggcctgtcag ggcgacggca
      841 tggtgaaggt ggaaatgcac tttcttcccg atgtgtatgt gccctgcgac gtgtgcgccg
      901 gccagcgcta caaccgcgaa acactggagg tgctctacaa gggcaagaac atcgcacaga
      961 ttctggacat gacggtggag gccgcctacg cgttcctgca ggctgtgccc accatcgccc
     1021 gaaaactcca gacactgctg gacgtcggcc tgtcctacat tcgtctgggg caggcggcca
     1081 ccacgctgtc gggcggcgag gcgcagcgtg tgaaactggc cctggagcta tccaaacgcg
     1141 acaccggccg caccctctac atcctggacg aacccaccac cggtctgcac tttgccgaca
     1201 tcgcgctact gctcaaagta ctgcaccagt tgcgcgatgc gggcaacacc attgtggtga
     1261 tcgagcacaa cctcgacgtc atcaagaccg ccgactggct catcgacatg ggcccggaag
     1321 gcggctctgg cggcggcacc gtggtgggcg tgggcacgcc cgaggacatt gcagccaatc
     1381 ccgcgagcca caccgggcgg tatttgaagc cgatcctgga aatacaagtg ccaaattggc
     1441 acctggcccc cgtggaatat gcgcaagcag ctataaaaag cgtagcggtt ggggtggtta
     1501 cgccgccatg aaccccaggt ccaccggata tgcgccgcca aagttgccga tattgggcaa
     1561 gactgtggcc atttccgcgc ccgggttgac gccaaaccac ttggccatgg tcgccgcata
     1621 ctggtccaca gacgtggtgg gcagcagacg cccctggccc acatgccact ggttctcggg
     1681 caggtagttg ccgcctgaat ccttggtgtc gctgatgctg actggcggcg ggctgccata
     1741 aaacgccttg cctttgaccg caccacccac cacgaaatgg tggctgcccc aaccgtggtc
     1801 cgaaccatcg ccattggatg cgagtgtgcg accgaagtcc gacgccgtga acgtggttac
     1861 ctggttggca atgcccattt catcggtagc gttgtagaac gcggtcatgg cactgctcac
     1921 ccgccccaga atggtggggt gttgtgcgat cagaccatcg tgcaaatcaa atccaccgat
     1981 cgacacaaag aacacctgcc tacgattgcc cagttgcgtc cggccgtgaa tcaggcgtgc
     2041 caccatcttc aattgctgcg ccagattgtt attggtcgtg tcaaaggcag ccacccaggg
     2101 tggtgcagag gctcccgcac tgagcgccaa ggccgacgtg atggcggctt cagagtccag
     2161 cgcgcgcttt gtcaccgtgt tgtagtcatt ctccagcttc tgggtgccgg tggtctgctg
     2221 gatcagggag ttgagggtgg aacgcaccac gctggacccg tacaccgccg tgtccgtcgc
     2281 ggggcgaatc ttgatggcgc caccggtact gacctggtac tgcagtgccg tatcccccga
     2341 cagaaacacc gcattgccgg acaccgaaat tcccgtgaac agggagttgg agttgaggct
     2401 ggggttggga ggaaccacca gatcgcccat gttgccaccc cagccaatct tggaaccctc
     2461 agggcgcgac gactgccaga tggactgctg gtcgttgtgg gaatacagct gtggcggcag
     2521 ggggtacagc gtgcgattgg agctgtggta ctgcgcacgc gtg
//
LOCUS       DAOSJM010000354         2563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_35068_length_2563_cov_5.437400, whole genome shotgun sequence.
ACCESSION   DAOSJM010000354 DAOSJM010000000
VERSION     DAOSJM010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35068_length_2563_cov_5.437400"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1972)
                     /locus_tag="PKX82_11165"
     CDS             complement(116..1972)
                     /locus_tag="PKX82_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HOF52859.1"
                     /translation="MFIFISRTLQIAGADVGLLRCSGMLRLAVLAVALVPAIYALIYL
                     SSVWDSNAKTGVLPVALVNLDEGIRYQGQVVNIGVELSDALLAKGIFGWRHVTDVDVA
                     RNAVATGRLAFAVIIPRDFSEQAVPGAVAGGGKIRVILSEGNNYSSAGFARRFAAELS
                     HQVNKTLSEKRWGMVLSTADGSQNSLIQLKVAVAQLRVGAKTLADGAVQYSAAATALS
                     GGFRQVASGVRVMEARLPAEDDLKPLNSGLQQLAAGQRELGRGLELLHTGSARLADGA
                     REMQVQSEGVPFFGSKLSKGAGALTAGAIQLGDGLGRARGANDQLTQGTAQLDAGVTR
                     LTDGVRALGGGVRTMAMRLPADEQLDAFSAGGKTLSDGATRLLDGIRQLDAVLPPAVG
                     KQRGSALGLADSVEPELENLAPVPNSGSAFAPNMLAVALWIGAVMSAYLFNMNLMPAS
                     LAGAPRLAQTVGKFIVPAMVTLLQASLVFLMLVFGLGLQTPHLHLINLLLTMAVASWA
                     FLAMLFAMLRVFGEASKLLAVLLLTLQLAAGGGVIPVELSGGLFQTVHDWLPLTWVVK
                     AFRASMFGAYDHGWADAWGAVILAGGVALLLAALIGRWKVVPDAEYRSGTEF"
     gene            complement(1996..>2563)
                     /locus_tag="PKX82_11170"
     CDS             complement(1996..>2563)
                     /locus_tag="PKX82_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOF52860.1"
                     /translation="YMERAIEKMKIVIPVTLLIIFLLLYLNFRRITETLIVMLSVPFA
                     LVGGVWLMWLLNYNLSVAVAVGFIALAGVAAETGVIMLIYLDHAWEDVKVKCRAAGRE
                     PGAGDLYGAVMEGAVERVRPKMMTVVAIMAGLLPIMWGTGTGSEVMSRIAAPMVGGMI
                     SSTVLTLAVIPALYALVKQWRLGKGLET"
BASE COUNT          562 a          851 c          709 g          441 t
ORIGIN      
        1 caaggcggcg gcaagagaaa gatcggcact tgattcggag atagtctgca agaactttga
       61 atttggtaga tgggaatttt ccccatattt attgccctga gctggatttg gcagctcaga
      121 attcggtgcc cgagcgatat tcagcatcgg gcacaacctt ccaacggcca atcaatgcag
      181 ccaacaggag cgcgaccccc ccggccagga tcacagcacc ccatgcatcg gcccatccgt
      241 ggtcataagc gccgaacatg ctggcccgaa aggccttgac cacccaggtg agaggcagcc
      301 agtcatgaac tgtctgaaac aggccgccgc tgagttccac cggaattacg ccgccaccgg
      361 cggccagttg cagggtcagc agcagcaccg ccagcagctt gctggcttca ccgaagacac
      421 gaagcatggc gaacagcatg gcgagaaacg cccacgacgc cacggccata gtgagaagca
      481 agttgatgag atgcaaatgg ggtgtctgca aacccagacc aaacaccagc atcaagaaga
      541 ccaggctcgc ctgcagcaac gtcaccatag ccgggacaat aaatttaccc accgtctggg
      601 ccaagcgcgg tgcacccgcc aagctggcgg gcatcaggtt catgttgaac agatacgccg
      661 acatgaccgc accgatccac agcgccactg ccaacatgtt gggcgcaaaa gcgctgccgc
      721 tgttgggaac cggggccagg ttttccaact cgggttcgac cgaatcggcc agcccgagtg
      781 cactgccacg ttgcttgccc actgctgggg gaaggactgc gtcgagctgc ctgatgccat
      841 ccagcaaccg ggtcgcgccg tcagaaagcg tctttccgcc tgccgagaag gcatcgagct
      901 gctcgtcggc tgggagacgc atcgccattg tccgcacacc gccacccagc gcccgcacac
      961 catccgtgag gcgggtcaca ccggcgtcca gctgcgccgt accttgcgtg agctgatcat
     1021 tcgcccctcg cgcccgaccc agcccatccc ccagttggat ggcccctgca gtcagggcgc
     1081 ctgcgccttt ggacagcttg ctaccaaaaa atggtacgcc ttcgctttgc acctgcatct
     1141 cgcgcgcgcc atcggccaac cgggcgctcc ctgtatgcaa tagctccagc ccacgaccca
     1201 gttcgcgctg gccagcggca agctgctgca agcctgagtt cagtggcttc aggtcatcct
     1261 ctgcaggcag gcgagcctcc atgacgcgaa cacccgatgc cacctggcgg aagccgccag
     1321 acaacgcggt ggccgcggca ctgtattgga cagccccgtc ggcgagggtc ttggcgccca
     1381 cgcgtaattg cgccacggct accttcagct gaatgaggct gttttgcgaa ccatcggcgg
     1441 tcgacaacac catcccccag cgcttttcgc ttagcgtctt gttcacctga tgactcaatt
     1501 cggcagcaaa gcgtctggcg aaacctgcag aagagtagtt gttaccctca gacaggatca
     1561 cccgaatttt tccgccgccc gcgactgcgc ctggcactgc ctgttcgctg aaatcccttg
     1621 gaatgatcac tgcaaaggcc aggcggcctg tggcaacagc gttgcgcgca acatcgacgt
     1681 ccgtcacgtg gcgccaaccg aagatgcctt tcgcaagcaa ggcatcgctt agctccacgc
     1741 cgatattgac gacttgaccc tgatagcgaa tgccttcgtc caggttgacc agcgccactg
     1801 gcagcacgcc agtcttggcg ttggagtccc agacgctcga aagatagatc agagcataga
     1861 tcgccgggac gagcgccacg gctagaaccg cgagccgtaa cataccgctg caacgcagta
     1921 ggcccacatc ggcgcctgca atttgcaggg ttctggaaat gaagatgaac atcaaaaaag
     1981 aagctcggac ttaacctatg tttccagtcc ttttccaagc cgccattgct tgaccagcgc
     2041 gtatagggcc gggatcaccg ccagcgtcag cacggtgctg gagatcatgc cgcccaccat
     2101 gggggcagca atccggctca tgacctcgga gcccgttcct gtgccccaca tgatcggcag
     2161 caggccggcc atgatcgcca cgacggtcat cattttgggc cgcacgcgct ccaccgcgcc
     2221 ctccatcacc gccccgtaga ggtcacccgc gccaggctcg cgcccggcag cgcggcattt
     2281 gaccttcacg tcctcccagg catgatccag gtagatcagc atgatcacgc cggtctccgc
     2341 ggccaccccg gccagggcaa taaagccgac cgccacggcc accgacaggt tgtagttgag
     2401 cagccacatc aaccataccc cacccacaag tgcgaagggg accgacagca tcacgatcag
     2461 cgtctccgtg atgcgccgga agttgaggta gagcagcaag aaaatgatca gcagcgtcac
     2521 cgggatcacg atcttcattt tctcgatggc gcgctccatg tac
//
LOCUS       DAOSJM010000355         2558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_35161_length_2558_cov_3.349181, whole genome shotgun sequence.
ACCESSION   DAOSJM010000355 DAOSJM010000000
VERSION     DAOSJM010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35161_length_2558_cov_3.349181"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PKX82_11175"
     CDS             complement(<1..998)
                     /locus_tag="PKX82_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 domain-containing protein"
                     /protein_id="HOF52861.1"
                     /translation="MFSRLHHLPRDARDTLFLLAVIAWIIAPQTAHLPPWCSAMAAGV
                     LGWRAVLAVQGSALPTRRWLVALLVLAMAGTYWSHRTLLGRDAGVTFVVVLLTLKTLE
                     MRARRDAFVIFFLGFFTLLSNFFFSQSLAVAASMLVGLLGLLTALVNSHMPVGRPPLR
                     QAAKTAGMMALLGAPIMAVLFMLFPRIAPLWGVPSDAMSGRSGLSATMQVGTIASLAL
                     DSSIAMRIRFEGAPPRQSDLYFRGPVLSTFDGREWRPLRSAFPPRFQLPANLQVQGDP
                     IRYQVTLEASSRPWILALDATPAKPEISGYTPSMQPDLQWMADRPIADLELGTRPT"
     gene            complement(1005..2072)
                     /locus_tag="PKX82_11180"
     CDS             complement(1005..2072)
                     /locus_tag="PKX82_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOF52862.1"
                     /translation="MAPPRSAVGASPSRGRTQWPGEASSTVAHTWNPISQVQRRLQIW
                     VESRIAPRDSVTLTQRNVYIVPTSAGWMLALTLLVLLVASINYQLNLGYVLTFLLTGS
                     AVVGMHICHGTLRGLTMHLIAPDAQFAGAGVPISINLSSERKSVRHGIGMALWGTDHW
                     SWTDVPAQGSTRVHVTYTPQQRGLHRLPTLTAETRFPLGTFRVWTVWRPAAQVLVYPA
                     PEAHPPPLPAGEPQAGGAGSAQRHATGEFDGVRAYRRGDPLKVVVWKKLAKADELISR
                     DAQHVQKLELWLDLAHTGSFPGTPATLEHRLSRLCAWVLLAQDRDIRYGLRLPGLEIA
                     PGSGEAHKRSCLQALALFQTA"
     gene            complement(2075..>2558)
                     /locus_tag="PKX82_11185"
     CDS             complement(2075..>2558)
                     /locus_tag="PKX82_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOF52863.1"
                     /translation="NPMDQIGTYALPESQLDRFLMRISLGYPDRAAERELLQGNDRRT
                     LLDTLPSLLDAGSLRALQQQVLAVHAAGPLLDYVQDLIAATRGGQWFLQGLSPRAGIA
                     VLRAAKALAFLSGRDYVAPDDVQAILPQTIAHRLIPVGDAGRGAEEQVRAMLAAVPLP
                     "
BASE COUNT          455 a          816 c          908 g          379 t
ORIGIN      
        1 tcggttgggc gcgtccccag ctcgaggtcg gcgatggggc ggtcggccat ccactgcagg
       61 tcgggctgca tgctgggcgt gtagccagag atctcgggtt tggcgggtgt ggcatccagc
      121 gccagtatcc agggccggct gctggcctcc agggtcacct ggtagcggat cgggtcgcct
      181 tgcacttgca ggttggcagg cagctggaag cgcggtggaa aggccgagcg cagcggccgc
      241 cactcgcgcc cgtcaaaggt ggacaacacg gggccacgga agtacagatc gctctggcgc
      301 ggcggcgcac cttcaaagcg gatgcgcatg gcaatgctgc tgtccagggc caggctggca
      361 atcgtgccca cctgcatggt ggccgacagg ccgctgcgtc cgctcatggc atcggacggc
      421 acaccccaca gcggcgcgat gcgcggaaac agcatgaaca gcaccgccat gatgggcgcc
      481 cccagcaggg ccatcatgcc ggccgttttg gccgcctggc gcaggggagg gcggcctacc
      541 ggcatgtggc tgttgaccag cgcggtcagc aggcccagca acccgaccag catcgaagcc
      601 gcaacggcca agctttgcga aaagaagaag ttggacagca gcgtgaagaa gcccaggaag
      661 aagatcacaa aggcgtcgcg gcgcgcgcgc atctccagcg tcttgagggt gagcagcacc
      721 accacaaacg tgacgccggc gtcgcgcccc agcagcgtgc ggtggctcca gtaggtgccc
      781 gccatcgcca gcaccagcaa ggccacgagc cagcggcgtg tcggcagtgc gctgccctgc
      841 accgccagca ccgcgcgcca gcccagaacg cccgcagcca tcgcgctgca ccacgggggc
      901 aggtgggcgg tttgcggcgc aatgatccag gcgataaccg ccagcaggaa cagcgtgtca
      961 cgggcatcgc ggggcaggtg gtgcaggcgg ctgaacatgg tggtctatgc cgtttggaat
     1021 aaggccagag cttgcaagca ggacctttta tgggcttcgc cactgccggg tgcaatctcc
     1081 aagccgggca ggcgcaggcc gtagcggata tcccggtcct gggccagcag aacccaggca
     1141 cacaggcggg agagccggtg ttccagggtg gccggggtgc cgggaaagct gccggtgtgg
     1201 gccaggtcca gccacagctc cagcttctgc acatgctgcg catcgcggct gatcagctcg
     1261 tcggccttgg ccagcttctt ccagaccacg accttgagcg ggtccccgcg gcggtaggca
     1321 cgcacgccat cgaactcgcc tgtggcgtgg cgctgtgcgc taccggcgcc gccagcctgt
     1381 ggctcgcccg caggcagggg cggcgggtgt gcctcgggtg cgggatatac caacacttgg
     1441 gcggcagggc gccagaccgt ccacacgcgg aaggtgccca gcgggaagcg cgtctcagcg
     1501 gtcagcgtgg gcaggcggtg cagcccacgc tgttgggggg tgtaggtgac atgcacgcgg
     1561 gtgctgccct gggcgggcac atcggtccag ctccagtggt cggtgcccca caacgccatg
     1621 ccgatgccat ggcgcacgga cttgcgttcg ctcgaaagat tgatagaaat tggcacccca
     1681 gcccccgcga actgggcgtc aggcgctatc aaatgcatag taagaccgcg cagggtgcca
     1741 tggcagatgt gcatgccaac caccgcgctg ccagtcagca ggaaggtcag tacatagccc
     1801 aggttgagct ggtagttgat gctggcaacc agcagcacca gcagggtcag cgccagcatc
     1861 cagccagcgg acgtgggcac gatgtagacg ttgcgctgcg tgagggtgac gctgtcgcgc
     1921 ggcgcaatac gcgactcaac ccagatctga agccgacgtt gtacctgcga tatgggattc
     1981 catgtgtggg caaccgtgga actggcttcg ccaggccact gggtgcgccc cctggagggg
     2041 gaggcgccaa cggcgcttcg ggggggagcc atatttacgg tagcggtacg gcggccagca
     2101 tggcccgaac ctgctcctcg gcgccacggc cggcatcacc cacgggaatg agccggtgtg
     2161 caatggtctg gggcaggatg gcctgcacat cgtcgggcgc cacgtaatca cggccgctta
     2221 agaacgccag tgccttggct gcgcgcagga cggcaatgcc ggcacgtggg ctcagcccct
     2281 ggagaaacca ctgcccaccc cgtgtggcgg caatcaggtc ctgcacatag tccagcagcg
     2341 gtcctgccgc gtggactgcc agcacctgct gctgcaaggc ccgcagtgag ccggcatcca
     2401 gcaggctggg cagggtgtcg agcagggtcc gccggtcgtt gccctgcagc agttcgcgct
     2461 cggccgcgcg gtccgggtag cccagcgaga tgcgcatcag gaatcggtcc agctgcgact
     2521 cgggcagcgc gtaggtgccg atctggtcca tcgggttc
//
LOCUS       DAOSJM010000356         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_35477_length_2539_cov_4.373994, whole genome shotgun sequence.
ACCESSION   DAOSJM010000356 DAOSJM010000000
VERSION     DAOSJM010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35477_length_2539_cov_4.373994"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..975
                     /gene="cyoE"
                     /locus_tag="PKX82_11190"
     CDS             79..975
                     /gene="cyoE"
                     /locus_tag="PKX82_11190"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOF52864.1"
                     /translation="MTKNVAAINPDVLRQFHALTKPRVIQLIVFCALIGMVLAVPGAP
                     SWSQVGLAALACLGIYLVAGAAAAFNCIVEKGIDAKMKRTAWRPTARGELGDWQTLIF
                     SAVLCIAGSVILYVWVNPLTMWLTFATFVGYAVIYTVILKPMTPQNIVIGGASGAMPP
                     VLGWAAMTGDVGPEAIILFLIIFLWTPPHFWSLALYRVEDYRKSGLPMLPVTHGSEFT
                     RLQIFLYTLILFSACLLPFVYGMSSWLYLAAAIVLSVGFCWYAWRLLRNYSDQLARET
                     FRFSLIHLSVLFAALLVDHYLV"
     gene            983..1594
                     /locus_tag="PKX82_11195"
     CDS             983..1594
                     /locus_tag="PKX82_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOF52865.1"
                     /translation="MQKRIAMKWIAACALSMGAMALLGACSEQKPSFVSIDVTGANYA
                     KGFELTDHNGQVRHLTDFPGKIVVMFFGYTQCPDVCPTSMAELTEVKKALGKDGDKLQ
                     GLFVTVDPARDTPAMLKEYMGNFDPTFLALYTTPEKLAELAKDYKVYYKKVDGKTPTS
                     YTMDHSAGSYVYDTQGQLRLFTRYGSGAPALTADIQLLLKEAR"
     gene            complement(1598..2539)
                     /locus_tag="PKX82_11200"
     CDS             complement(1598..2539)
                     /locus_tag="PKX82_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOF52866.1"
                     /translation="MSTGATGQINQKTAAAWPTRPLRIVVGFPGGSSPDLTARALAEP
                     LSKALGQPVIVENRVGAAGNIAADFVAKATDNHTLGLMINGNMTIAKLLNPRLPYDPL
                     QDLKPVSLIGTSPLVLVAPAHAAGATARDVLANARNAGNTWNYGTPGVGTVGHLGMEL
                     LKSRAGVAPVHVPYSGYPQVATAMVSGDLQLALLPPALAMAQIRAGKLRAIGMTASVR
                     STLVPDIPSLSEAGVRDFNLEIWNAVAAPNSMPPAVVARLSALVSEIVRTPEMRQKLF
                     QQGWNAVGTSAQGLANRIQADTVVLGGIIASQRITAE"
BASE COUNT          442 a          782 c          795 g          520 t
ORIGIN      
        1 tgctggtgtg gcttctggcc gccaccggaa cccaagagac tttggagaga gctgcatcca
       61 cgcggccaag ctatgcccat gactaagaac gttgccgcga tcaacccgga cgtgctgcgc
      121 cagttccacg cactgaccaa gccgcgcgtc attcaattga tcgtgttttg cgcgctcatt
      181 ggtatggtgc tggcggtgcc gggtgcgccc agctggtcgc aggtcgggct ggctgcgctg
      241 gcctgcctgg gtatttacct ggtggcaggt gcggcggcgg ctttcaattg catcgttgaa
      301 aaaggcatcg atgccaagat gaagcgcacc gcctggcggc ccacggcccg gggtgaactg
      361 ggcgattggc agaccctgat cttttcggcg gtactgtgca tcgcaggctc ggtgattctc
      421 tacgtctggg tcaacccgct gaccatgtgg ctcacctttg ccacgtttgt gggctacgcg
      481 gtgatttaca ccgtgatcct caaacccatg acaccccaga acatcgtgat cggcggcgca
      541 tcgggtgcca tgccgcccgt gctgggctgg gcggccatga ccggcgatgt agggcccgag
      601 gccatcatcc tgttcctgat catcttcctg tggacgccgc cgcatttctg gtccctggcc
      661 ctgtaccggg tggaggacta ccgcaagtcc ggcctgccca tgctgccggt cacgcacggc
      721 agcgaattca cgcggctgca gatatttctt tacaccctga tattgttttc tgcctgcctg
      781 ctgccgtttg tgtacggcat gagttcgtgg ctgtacctgg ctgcggccat tgtcctcagc
      841 gtggggttct gctggtacgc ctggcggctg ctgcgcaact actcggacca gcttgcgcgc
      901 gagactttcc gcttttccct gatccatctg agcgtgctgt ttgcagcgct gctggtggac
      961 cactaccttg tttaggaatt ccatgcaaaa acgcattgct atgaaatgga tagctgcttg
     1021 cgcattatcc atgggggcta tggccttgtt aggtgcctgc tctgaacaga agccgtcatt
     1081 cgtctcgatt gatgtgacgg gtgcgaacta tgccaaagga ttcgagctga ccgaccacaa
     1141 cggccaggtg cgccacctga ccgatttccc cggcaaaatc gtggtgatgt tctttggcta
     1201 cacgcaatgc cccgacgtct gccccacatc catggccgaa ctgaccgagg tgaaaaaggc
     1261 actgggcaag gatggcgaca agctgcaggg cttgtttgta acggtagacc ccgcacggga
     1321 cacgccggcg atgctcaagg agtacatggg taacttcgac cccactttcc tggcgctcta
     1381 caccacgccc gagaagctgg ccgagctggc aaaggattac aaggtgtact acaagaaggt
     1441 ggatggcaaa acgcccacca gctacaccat ggaccactcg gcaggcagct acgtctatga
     1501 cacccaaggc cagttgcgcc tgttcacccg ctacggcagt ggtgcgccgg ccttgaccgc
     1561 cgacatccag ttgctgctga aagaagcgcg ctaacggcta ctccgctgta atgcgctggc
     1621 tggcaatgat gccgcccagt accacggtgt cggcctggat gcggttggcc agtccctgtg
     1681 cagacgttcc caccgcgttc cagccctgct ggaacagctt ttgccgcatc tccggcgtgc
     1741 ggacgatctc gctgaccagt gcagacaggc gcgccaccac agcgggaggc atgctgttgg
     1801 gggcggctac ggcgttccag atttcgagat tgaagtccct cacgccggct tcagacaggc
     1861 tgggaatgtc cggcaccagc gtgctgcgca cactggcggt catgccaatc gcgcgcagtt
     1921 tgcccgcgcg gatctgcgcc atggccaggg ctggtggtag cagggcgagc tgcaggtccc
     1981 ctgacaccat ggcagtggcc acctgcgggt agccagagta gggcacatgc accggggcca
     2041 cgccggcgcg gctcttgagc aactccatgc ccaggtggcc caccgtgcca acgcccggcg
     2101 tgccgtagtt ccaggtgttg cccgcattgc gggcgttggc cagcacatcc ctggccgtgg
     2161 cacccgcagc atgggcggga gctaccagca ccagcggcga ggtgccaatc aggctgaccg
     2221 gcttcaggtc ctggagcggg tcatagggca gtcgggggtt gagcagtttg gcaatcgtca
     2281 tgttgccgtt gatcatcagc ccgagggtgt ggttgtcggt cgccttggcc acgaagtcgg
     2341 cagcgatgtt gcccgctgcg cccaccctgt tttccacgat caccggctgg cccagcgctt
     2401 tggacagcgg ttccgccaac gcgcgggccg tcaggtcggg cgacgagcca ccgggaaagc
     2461 ccacaacgat gcgcagcggc ctggtcggcc aggcagcggc agttttttga ttgatttggc
     2521 ctgtagcccc cgtggatat
//
LOCUS       DAOSJM010000357         2538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_35501_length_2538_cov_4.287958, whole genome shotgun sequence.
ACCESSION   DAOSJM010000357 DAOSJM010000000
VERSION     DAOSJM010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35501_length_2538_cov_4.287958"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /gene="hflK"
                     /locus_tag="PKX82_11205"
     CDS             <1..745
                     /gene="hflK"
                     /locus_tag="PKX82_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOF52867.1"
                     /translation="YRLSDARAFLFESKNPADAVVQAAETAVREVLGKMKMDAALSEE
                     RDQIAPRVRALMQGILDRYKVGVEVVAINLQQGGVRPPEQVQASFDDVLKAGQERERA
                     KNEAQAYANDVIPRAVGSASRLKEEADAYKARVIAQAQGDAQRFRSVYTEYQKAPQVT
                     RDRMYLDTMQQIYGNVTKVLVESRQGSSLLYLPLDKILQMNGSNGASAEAAALPATST
                     PAPAAPVTSTLNSDARSRDAARTRERDSR"
     gene            755..1657
                     /gene="hflC"
                     /locus_tag="PKX82_11210"
     CDS             755..1657
                     /gene="hflC"
                     /locus_tag="PKX82_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOF52868.1"
                     /translation="MNRIGLIVSSLLVLIALASSTLFVVDQRQFGVVYALGQIKDVIT
                     EPGLNFKLPPPFQNVSYIDKRLLTLDSTDAEPMLTAEKQRVVIDWYVRWRITEPTEYI
                     RNVGLNESAGAQQLNRVVRNAFQEEINKRTVKELLSLKREALMADVKAEVLDKVRGAK
                     PWGVDVVDVRITRVDYVEAITESVYRRMEAERKRVANELRSTGGAEGEKIRADADRQR
                     EVTIANAYRDAQKIKGEGDAEAARIYGEAFGRDPQFAQFYRSLEAYKASFANKSDVMV
                     LDPASSEFFKVFRNGGSAAGAAKK"
     gene            1661..1852
                     /locus_tag="PKX82_11215"
     CDS             1661..1852
                     /locus_tag="PKX82_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOF52869.1"
                     /translation="MDSGTLWLALALVFIFEGLFPFAAPGAWRRMFTQLLQLSDGQIR
                     FFAVISISLGLTLIWWLVP"
     gene            1907..>2538
                     /locus_tag="PKX82_11220"
     CDS             1907..>2538
                     /locus_tag="PKX82_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOF52870.1"
                     /translation="MSAWVLPDHIADVLPFEARHIEEIRRDLLDMARCYGYELVMPPL
                     LEHLESLLSGTGEALDLQTFKLVDQLSGRMMGLRADSTPQVARIDAHLLNRSGVTRLC
                     YCGPVLHTRPGAPHATREPLQFGAEIYGHKGLEADLEILTLALDALQAAQVNAVSVDM
                     ADARIVHALIAQTQLGSDKRALLHAALAAKDASEIQSLTRGCPAAPAQGL"
BASE COUNT          507 a          736 c          781 g          514 t
ORIGIN      
        1 ataccgtttg agcgatgcgc gggctttcct gtttgaaagc aagaatccgg cggatgccgt
       61 ggtccaggct gctgagactg cagtacgcga agtgctgggc aagatgaaga tggatgcagc
      121 actgtccgaa gagcgtgacc agattgcgcc tcgcgtgcgt gcgctgatgc agggcatcct
      181 cgaccgctat aaagtgggtg tggaagtcgt ggccatcaat ctgcagcagg gcggtgtgcg
      241 tcctcctgag caggttcagg cgtcatttga cgatgtactc aaggccggac aagagcgcga
      301 acgcgccaaa aatgaggcgc aagcctatgc gaacgacgtg atcccccggg ctgtgggttc
      361 tgcatcccgc ctgaaggaag aagccgatgc ctacaaggcg cgggtgatcg cgcaggcaca
      421 aggcgatgcg caacgtttcc gctccgtgta tacggaatac cagaaggcgc ctcaggtcac
      481 ccgcgaccgc atgtacctcg acaccatgca acagatctac ggcaacgtga ccaaagtgct
      541 ggtggaatcc aggcaggggt ccagcctgct gtaccttcct ctggacaaga ttctgcagat
      601 gaatggcagt aacggcgcct ccgcggaagc ggcagcactt ccggccacca gcacgccagc
      661 gcctgctgcg ccagtgacca gtactttgaa cagtgatgcg cgtagccgtg atgcggcccg
      721 cacgcgtgaa cgtgattcac gctaggagta acgaatgaac agaattggat tgattgtttc
      781 gtccttgctg gtgctgattg cactggcgag ttcgaccctg tttgtagtgg accaacggca
      841 gtttggcgtg gtgtacgcct tgggccagat caaggacgtc atcaccgagc cgggtctgaa
      901 tttcaaattg cccccgccat tccagaacgt ctcctacatt gacaagcgtc tgctgacgct
      961 cgacagcacc gatgcggagc ccatgctcac ggcagaaaag cagcgcgtgg ttatcgactg
     1021 gtacgtgcgc tggcgcatta ccgagccgac cgagtacatc cgcaatgtgg gtctcaatga
     1081 gagcgcgggt gcccagcagc tcaaccgcgt ggtgcgcaat gcattccagg aagaaatcaa
     1141 caagcgcacg gtaaaggaac tgctgtccct caagcgcgaa gccctgatgg ctgacgtcaa
     1201 ggccgaagtg ctggacaagg tccgtggtgc caagccctgg ggtgtcgatg tggtggatgt
     1261 acgcatcacc cgcgtggact atgtggaagc cattaccgaa tcggtctacc gccgcatgga
     1321 agccgaacgc aagcgcgtcg ccaatgaatt gcgctctacc ggtggcgccg agggtgagaa
     1381 gattcgcgcc gatgccgatc gccagcgcga ggtgacgatt gccaatgcct accgcgatgc
     1441 ccagaagatc aagggcgagg gtgatgccga ggccgcccgt atttatggcg aggcctttgg
     1501 acgtgacccg cagtttgcgc agttctatcg cagcctggag gcgtacaagg ccagctttgc
     1561 caacaagagc gatgtaatgg tgctggatcc ggcgtcttcc gagttcttca aggtattccg
     1621 caacggtggt tctgccgctg gcgctgccaa gaagtaagac ttggatagcg gtaccctctg
     1681 gctggcactg gccctcgtat tcattttcga gggcctgttt ccttttgcgg cccctggtgc
     1741 ctggcgccgc atgttcaccc agctgctgca actgtcggat ggacagatcc gttttttcgc
     1801 agtgatcagc atttcgctcg gtctgacgct gatttggtgg ctggttccgt aaattcccgg
     1861 gtccaagccc ggtaaaatca cggttttaac agctccccct aattccatgt ctgcctgggt
     1921 cctgccggat cacattgccg atgtgctgcc ctttgaggcc cgccacatcg aagaaatacg
     1981 tagagacctt ctggacatgg cacgttgcta tggctacgag ctggtcatgc ccccgctgct
     2041 ggaacacctg gagtcgctgc tctcgggtac cggtgaagcg ctggacctgc aaaccttcaa
     2101 acttgtcgac cagctgtccg gtcgcatgat gggtttgcgg gccgacagca cgccccaggt
     2161 tgcccgtatt gatgcgcacc tgctgaaccg ttctggtgtc acccgtctgt gctactgcgg
     2221 gccggtgttg cacacccgtc cgggtgcgcc gcacgcgacc cgcgagcctt tgcagttcgg
     2281 tgcagaaatc tatggccaca agggcctgga agccgatctt gaaatcctca ctctcgcact
     2341 ggatgcgctg caagcggcgc aggtcaatgc ggtgagtgtg gacatggccg atgcgcgcat
     2401 cgtgcatgcc ctgatcgcgc agacccagtt gggctccgac aagcgggcct tgctgcatgc
     2461 cgcactggca gccaaggacg caagcgaaat ccagtccctg acccgcggtt gccccgctgc
     2521 gcctgcgcag gggctgtg
//
LOCUS       DAOSJM010000358         2526 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_35720_length_2526_cov_3.637798, whole genome shotgun sequence.
ACCESSION   DAOSJM010000358 DAOSJM010000000
VERSION     DAOSJM010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35720_length_2526_cov_3.637798"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKX82_11225"
     CDS             complement(<1..528)
                     /locus_tag="PKX82_11225"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HOF52871.1"
                     /translation="MLDRDGFRPNVGIVLLNQRNQVFWGKRIRTHSWQFPQGGIDRGE
                     TPEQAMFRELHEEVGLQPEHVSIVARTRDWLRYEVPDRYIRRDARGHYKGQKQIWFLL
                     QLTGHDWDLNLRATDHPEFDAWRWNDYWVPLDAVVEFKRGVYEMALTELARYLPRNDP
                     RNRYLRHNLRGRDHEG"
     gene            620..2362
                     /locus_tag="PKX82_11230"
     CDS             620..2362
                     /locus_tag="PKX82_11230"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOF52872.1"
                     /translation="MKASQFLISTLKEAPADAEVVSHKLMMRAGMIKKLGAGIYSYMP
                     MGLRVIRKVEAIVREEMNRAGAIEMTMPVVQPAELWQETGRFEKMGPELLRIKDRHDR
                     DFVIQPTSEEVVTDIARQEIKSYKQLPKNFYQIQTKFRDERRPRFGLMRGREFTMKDA
                     YSFDRDQDAAKASYQVMAGAYRKIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGE
                     DAIVYCPGSEYAANMEKAEALAPAGPRPAPSQAMTQTPTPGKSTCEDVAALLNVPLST
                     TVKSLVLATDSKDDKGNVTKTQVWLLLLRGDHDMNEVKVGKLDGLADFRFATVPEIEE
                     HFGCKPGYLGPINLQKPLKIVADREVAVMADWICGANAVDFHITGVNWGRDLPEPDMV
                     ADLRNVVAGDASPDGKGILHIERGIEVGHVFYLGTKYSKAMNATFLDVNGKPQFMEMG
                     CYGIGITRLPAAAIEQNHDDKGIIWPDSIAPFTVALCPISPDRFPEVKAAADKLYADL
                     LAAGVDVILDDRNERPGAMFADWELIGVPHRVNIGDRGLKEGNVEYQHRRDEKATNVP
                     VSEILGLLQSRLKA"
BASE COUNT          543 a          726 c          768 g          489 t
ORIGIN      
        1 tccttcatgg tcgcgaccac gcaggttgtg gcgcaggtac cggttgcgcg ggtcgttgcg
       61 cggcaggtag cgcgccagtt ctgtgagcgc catctcgtac acgccacgtt tgaactccac
      121 cacggcatcg agcggcaccc aataatcatt ccagcgccag gcatcaaact ccgggtggtc
      181 ggtggcgcgc aggttcaaat cccagtcgtg ccccgtcaac tggagcagaa accagatctg
      241 tttttggccc ttgtagtggc cgcgtgcatc gcggcgaatg taccgatccg gcacctcgta
      301 gcgcaaccag tctcgggtac gggcaacaat gcttacatgc tccggctgga ggcccacttc
      361 ttcgtgcaac tcgcggaaca tggcctgttc cggggtttca ccccggtcaa tgccaccctg
      421 cgggaattgc cacgagtggg tacgtatgcg cttgccccaa aatacctgat ttctctggtt
      481 gagcaggacg atgccgacgt tgggcctaaa gccgtcccgg tcaagcataa tcaaacccca
      541 atttttaaac ttcgttcatt atgcacagcg attgctgtgg atcaaggggc gaagaccctc
      601 gtaggtcaca agtcacgcca tgaaagcatc ccaatttctc atttccaccc tcaaagaagc
      661 gcccgccgat gcggaagtgg tcagccacaa gctgatgatg cgcgcgggca tgatcaagaa
      721 gctgggtgcc ggcatctaca gctacatgcc catgggcctg cgcgtgattc gcaaggttga
      781 agccatcgtg cgggaggaaa tgaaccgcgc gggtgccatc gagatgacca tgccggtggt
      841 gcaacccgca gagctgtggc aggaaacagg ccgcttcgag aagatgggcc ccgaactgct
      901 gcgtatcaag gatcgccacg accgcgactt tgtgatccag cccaccagcg aagaggtggt
      961 gacggacatt gcgcgccagg agatcaagag ctacaagcag ttgcccaaga atttctacca
     1021 gatccagacc aagttccgtg acgagcggcg cccccgtttt gggttgatgc gtgggcgcga
     1081 gttcacgatg aaagatgcct acagttttga ccgtgaccag gacgctgcca aggccagcta
     1141 ccaggtcatg gcgggtgcgt accgcaagat ttttgaccga tttggtctga cctaccgcgc
     1201 ggtggcggct gacagcggcg ctattggcgg tgatttgagc gaagaattcc aggtgattgc
     1261 ggccaccggt gaagacgcca tcgtgtactg ccctggcagc gaatacgcgg ccaacatgga
     1321 aaaggccgaa gccctggcac ctgcgggtcc gcgtccggca cccagccagg cgatgaccca
     1381 aacaccgaca ccgggaaaga gcacctgcga agacgtcgcc gcgctgctga acgtgccgct
     1441 gtccacgacc gtcaaatcgc tggtgctggc caccgatagc aaggacgaca agggcaatgt
     1501 caccaagacc caggtctggc tgctgctcct gcgcggcgac catgacatga acgaagtcaa
     1561 ggtgggcaag ctcgacggcc tggccgattt ccgttttgcc actgtgcctg agattgaaga
     1621 gcattttggt tgcaagccgg gttacctggg ccccatcaat ctccagaagc cgctgaaaat
     1681 tgtggcggat cgcgaagtgg cggtcatggc tgactggatt tgcggtgcca atgcggtgga
     1741 tttccatatc accggcgtga actgggggcg agacttgccc gaacccgata tggtggccga
     1801 cctgcgcaat gtggtggcgg gcgatgcatc acccgacggc aaaggcatct tgcacatcga
     1861 acgtggcatc gaggtgggcc acgtctttta cctgggaacc aagtacagca aggcgatgaa
     1921 tgcgaccttt ctggacgtca acggcaaacc ccagttcatg gaaatgggtt gttatggaat
     1981 tggcatcacg cgcttgccgg ctgctgcgat tgagcagaac cacgacgaca agggcatcat
     2041 ttggcccgat tccattgcac cgttcaccgt agctctgtgc cccatcagtc cagaccgttt
     2101 ccccgaggtg aaggctgctg ccgacaagct gtacgccgac ctgctggccg ctggtgtgga
     2161 cgtgatcctg gacgaccgca acgagcgccc tggagccatg tttgcggatt gggaactcat
     2221 cggcgttcca caccgcgtga acattggcga ccgtggcctc aaagagggca acgtggagta
     2281 ccagcaccgc cgcgatgaga aggccaccaa tgtgccggta tccgaaatcc tgggtctgct
     2341 gcagtctcgt ctgaaagcat gatccatcgg ggtgtggagg ctggaattaa gaggcgaaac
     2401 gtcctcctgc ccccgttggt attgggtggt ttgctacttt ttcaggagcg cgccaaagcg
     2461 ggagcccaaa gggaagagcc gatagtcgat gcggttcgga acgccatgag tgcctctgtc
     2521 aaaagc
//
LOCUS       DAOSJM010000359         2522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_35801_length_2522_cov_4.661127, whole genome shotgun sequence.
ACCESSION   DAOSJM010000359 DAOSJM010000000
VERSION     DAOSJM010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35801_length_2522_cov_4.661127"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PKX82_11235"
     CDS             complement(<1..259)
                     /locus_tag="PKX82_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HOF52873.1"
                     /translation="MNTLLPLRLLVAPDKNDPTPLVLYHGRSCPDGFAAALAARLYYG
                     DAVECLGLDHGDIKTVDDLPPLQGRAVYILDFSFDAPILKDI"
     gene            337..996
                     /locus_tag="PKX82_11240"
     CDS             337..996
                     /locus_tag="PKX82_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOF52874.1"
                     /translation="MSASWYDRHILPTALDTACGMPMISRMRQRIVPLARGRVLEVGI
                     GTGLNMRHYDKTRVTRITGLDPALQLHPLARERIAQAGLEVDLVGLSAEAIPLADASF
                     DTVVVTYTLCTIPDPLSALREMHRVLVPDGRLLFCEHGRAPDASVKAWQDRLQPLWGK
                     VSGGCHLGRDIPELLQQTGFSVPELQTRYLRGPRPFTFHYWGEALPVCRQNSMSNSSP
                     K"
     gene            999..1580
                     /locus_tag="PKX82_11245"
     CDS             999..1580
                     /locus_tag="PKX82_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HOF52875.1"
                     /translation="MIKLALPLATALLMVVAGCSQQDAPAKPAATASNPAQSYADVAA
                     KTKGFTVGAMMSANTVYVLFDPQCPHCGHLWQQSQPLLKKVKFVWIPVSFINAKSTPQ
                     GAALLTAGNPAELMTTHETSILAGTGGIAASASIPPEVETAIKTNTQLLNSMGVESVP
                     YIMAKHAASGQVVTNAGAMETAALAKLLGVEIP"
     gene            1658..>2522
                     /locus_tag="PKX82_11250"
     CDS             1658..>2522
                     /locus_tag="PKX82_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOF52876.1"
                     /translation="MKRLCTSRRWAVAGLAALWASTSLGVAAQTTWPTKPVRIVVPFA
                     PGGTTDILARAIAPELSKAFGQQFIVENKAGAGGNLGAEVVAKSASDGYTLLMGTVGT
                     HGINRSLYPRLPYDPFKDFAPVTLVAGVPNVMVVNADMAKANNLQSVQDFIKLAKSRP
                     GKLNMASSGNGTSIHLAGELFKSQTGLFMTHIPYRGSGPALMDLAGGQMDVMFDNLPS
                     AMQLIKSGKLKALAVTSAQRSAALPDVPTVEEAAGLKGFEATSWFGLLAPAGTSPEIV
                     SRIQQEVARALN"
BASE COUNT          547 a          765 c          714 g          496 t
ORIGIN      
        1 caatatcttt cagaatcggc gcatcgaatg aaaaatccag gatatacacg gccctgccct
       61 gcaaaggcgg caagtcatcc acggtcttga tatccccgtg gtcaagcccc aggcattcca
      121 cggcatcgcc gtaatacagg cgcgccgcga gcgcggcagc gaatccatcc ggacagctgc
      181 gtccgtggta caaaaccagc ggagtggggt cattcttgtc aggggccacg agaagccgca
      241 aggggagtaa ggtattcatg gggctactgt accgcctcgg caccgcacca ggcgccgcaa
      301 gtcacaatag cgcgaacaac cagcccgaag agcaccatgt ccgcatcctg gtacgaccgc
      361 cacatcctgc ccaccgccct ggatacggcg tgtggcatgc ccatgatttc acgcatgcgc
      421 caacgcattg ttccgttggc ccgtgggcga gttctggagg tgggcatcgg gactgggctc
      481 aatatgcgcc attacgacaa aacgcgcgtc acccgcatta ctggactgga tcccgccttg
      541 caattgcatc cactggccag agagcgcatt gcgcaagcag gcctggaagt cgatttggtg
      601 ggtctgtccg ctgaagccat tccgctggca gatgccagtt ttgacaccgt tgtggttacc
      661 tatacgctgt gcaccattcc cgatccattg agcgctctgc gcgaaatgca cagggtactg
      721 gtgccggatg gcaggctgct gttttgtgaa catggccgcg caccggatgc gtcggtcaag
      781 gcctggcagg ataggttaca gcctctttgg ggaaaggtct ctggaggatg tcacctcgga
      841 cgggacattc cagaactgct gcaacaaacc ggcttctccg tgccagagct gcagacgcgc
      901 tatttgcggg gtccgcgccc cttcacattc cactactggg gcgaggctct cccggtttgc
      961 aggcagaatt caatgtccaa ctcatcccca aaatgaaaat gatcaaactt gccttgcccc
     1021 ttgccacagc cctgctgatg gtcgttgccg gttgttccca gcaggatgca ccggccaagc
     1081 ctgcggccac tgcctccaat ccggcgcagt cctatgcgga tgtagcggcc aagacaaagg
     1141 gattcacggt aggcgccatg atgagtgcca atacggtgta tgtacttttt gatccccagt
     1201 gcccgcattg cggccacctt tggcaacaat cccaacccct gctcaagaaa gtcaaatttg
     1261 tgtggatccc ggtctccttc atcaatgcaa aaagtacgcc gcaaggcgcc gctttgctga
     1321 cagcaggaaa cccggcggaa ctgatgacaa cccatgaaac atcgatcctg gcaggtacgg
     1381 gtggcatagc cgcctcagcc agtattcccc ctgaggtgga aactgccatc aaaaccaaca
     1441 cgcagctgct caacagcatg ggtgtggagt cggtcccgta catcatggcc aaacatgcag
     1501 caagtgggca ggttgtcacc aatgctggcg ccatggagac ggctgccctg gcgaagcttc
     1561 tcggcgtgga aattccctga gactgtcacc caggtttggg ggccttgcgg gatttgctgg
     1621 catgatgttc ggcaaatact acaagaggag acacaccatg aaacgcctat gtaccagccg
     1681 ccggtgggcc gttgccggat tggcagcgct atgggcttcc accagtctcg gagtggcagc
     1741 ccagactacc tggccaacca agccggtcag gattgtggtt ccttttgcgc caggtggcac
     1801 taccgatatc ctcgcgcgtg ccattgcgcc agaactctcc aaagctttcg ggcaacagtt
     1861 catcgtggaa aacaaggcgg gtgccggggg caacctgggt gccgaggtcg tcgccaaatc
     1921 agccagtgac ggttacacgc tgctgatggg taccgtgggc acacatggca tcaaccgctc
     1981 gctgtacccc cgattgccct atgacccgtt caaggacttc gctcccgtca cgctggtagc
     2041 aggggtaccc aacgtcatgg tggtgaatgc cgacatggca aaggccaaca acctccaatc
     2101 cgtccaggac ttcatcaagc tggccaaatc ccgccccggc aagctcaaca tggcgtccag
     2161 tggcaacgga acatccatcc atctggcggg tgaattgttc aagagtcaga ccggattgtt
     2221 catgacccac attccctacc ggggttccgg ccccgcattg atggatctgg caggcggcca
     2281 gatggacgtc atgttcgaca acctcccttc ggccatgcaa ctcatcaaaa gtggcaagct
     2341 caaggccttg gctgtcacca gcgcacagcg ctcggctgct ctgcccgatg tacctacagt
     2401 ggaagaagcc gcgggactca aaggtttcga agcaaccagc tggtttggtc tgctggcccc
     2461 ggcgggcact tcgccagaga ttgtgagccg cattcagcag gaggttgccc gcgcgctgaa
     2521 ta
//
LOCUS       DAOSJM010000360         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_36080_length_2506_cov_3.561404, whole genome shotgun sequence.
ACCESSION   DAOSJM010000360 DAOSJM010000000
VERSION     DAOSJM010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36080_length_2506_cov_3.561404"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PKX82_11255"
     CDS             <1..548
                     /locus_tag="PKX82_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOF52877.1"
                     /translation="WAKADFDDQTVIALYPADGVQRQLLVAPAGSPPPEGNGLPLEVW
                     QWGEVRSAVATLSTPVFEAFVPQMLNYESVGGVNFKKGCYPGQEVVARSQFRGTLKRR
                     AFVFHCDAPLAAGDEVFSAGDESQPAGVVVQAAHAPSGGFDALISVQLNALDTGSLHL
                     KSASGPGLLQLPLPYSLLDDI"
     gene            610..1239
                     /locus_tag="PKX82_11260"
     CDS             610..1239
                     /locus_tag="PKX82_11260"
                     /inference="COORDINATES: protein motif:HMM:NF016247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HOF52878.1"
                     /translation="MSTPLTKEEAFARLGSITREMHEALTVLGGNQLHSIVEEIPNAR
                     DRLAYVGKMTEDAANKVLTLVEKAKPECDDLSTRGLELSQSLTRMAGTQDMSVDRAKG
                     LLTVCGKFAESTAKFAGDQANVLSDIMMAQDFQDLSGQVIKKVIDIITRTEMQLVQLL
                     IDSSPETVAVPKSDAQEITVDNHKLEGPQTDGTALKQDDVDDLLASLGF"
     gene            complement(1243..2106)
                     /locus_tag="PKX82_11265"
     CDS             complement(1243..2106)
                     /locus_tag="PKX82_11265"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HOF52879.1"
                     /translation="MNPKRADFRCLHRLRVRWAEVDMQKIVFNAHYLMYFDTAMADYW
                     RELAVPYEAAMHMLNGDLYVKKASVEYFSSARYDDQLEIGLRCGRIGNSSIIFDGAIF
                     RGDTLLVTGELIYVFADPATQTSRPVPDALRDLLMAYESGQSMVRVETGNWATLESQA
                     SPLRQAVFVQEQGTDAALVWDAADADAVHAILINRLGMAVASGRLVQQAPGVGRIGRM
                     AVDRVLRGSSLGRAMLDALVSSARSRGDKEVMLHAQRSAEGFYRRLGFVARGAPFEEA
                     GLDHIEMVLAL"
     gene            complement(2103..>2506)
                     /locus_tag="PKX82_11270"
     CDS             complement(2103..>2506)
                     /locus_tag="PKX82_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF52880.1"
                     /translation="PGVAGHQDTPSHHTFDKGFVLEEPHITYFFNHYVRTQADRDDWR
                     FAPLDGLTPSGHVADLSGVAPAWLGLAECDPLVDEGVLYADQLRMAGVAVDLEIYRGV
                     VHEFIKMGRAIPEAEKAHADAARALKEAFAT"
BASE COUNT          519 a          744 c          754 g          489 t
ORIGIN      
        1 cgtgggctaa ggccgatttt gatgatcaaa ccgtgattgc cctctaccct gcggacggcg
       61 tgcagcgcca actgctggtg gcgccagcag gcagtccacc gcccgaaggc aatggcttac
      121 cgttggaagt ctggcagtgg ggcgaagtgc gcagcgcggt ggccacgttg agcacccccg
      181 tatttgaggc ttttgtcccc cagatgctga actacgagtc cgtgggcggg gtgaatttca
      241 agaagggttg ctatcctggg caggaggtgg tggcacgtag ccagttccgt ggcacgctca
      301 agcggcgtgc attcgtgttt cattgtgacg ctccgctggc tgcgggagat gaagtgtttt
      361 ccgcaggtga cgagtctcag ccggcgggtg tggtggtgca ggctgcccat gccccctcgg
      421 gtgggtttga cgccttgatt tctgtgcagt taaacgcgct ggatacaggc agcctgcatt
      481 tgaaatccgc cagtggcccc ggcctgctgc aattgccgct gccgtattcg ttgctggacg
      541 atatttaggt ccggaactcc ctgtaaacta accattcagg cttgcgccaa gaagggattg
      601 gttccataca tgtccacacc actgacaaaa gaagaagcgt tcgcaaggtt gggttccatt
      661 acccgggaaa tgcacgaagc gttgaccgta ttgggcggta accagttgca cagcattgtg
      721 gaagaaattc ccaacgcccg cgaccgcttg gcctatgttg gcaagatgac tgaagacgct
      781 gccaacaaag tgctcaccct ggtggaaaaa gccaaaccgg agtgtgacga tctgtctacg
      841 cgtggactgg aattgagcca gtcgctgaca cgcatggcag gcacccagga catgagcgtg
      901 gaccgtgcca aaggtcttct gacggtgtgc ggaaagtttg cagagagcac cgccaagttc
      961 gccggcgacc aggccaatgt gctgagcgac atcatgatgg cgcaggattt ccaggatttg
     1021 tcagggcagg tcatcaagaa ggtgatcgac atcattaccc gcaccgagat gcagctggtg
     1081 cagctgctga tcgacagttc gcctgaaacg gtggcagtgc ccaagtctga tgcgcaggaa
     1141 atcaccgtgg acaaccacaa gctcgaagga ccgcaaaccg atggtacggc gctgaaacag
     1201 gacgacgtgg acgacctgct cgcctcactc ggcttctagc ctttacagcg ccaggaccat
     1261 ttcaatgtgg tccagccctg cctcttcaaa cggcgctccg cgcgccacaa aacccaatct
     1321 ccggtagaag ccttcggcgc tgcgttgtgc gtgcagcatg acttccttgt cgccacggct
     1381 gcgcgcactg gaaaccagcg catccagcat ggcacgcccc agactggagc cacgcagcac
     1441 ccggtccacg gccatccggc caatgcgccc tactccgggt gcctgttgca ccagtctgcc
     1501 actggccacg gccattccca gccggttgat gagaatcgca tgcacagcat ccgcatcggc
     1561 tgcgtcccac accagcgctg catcagtgcc ctgttcctgc acaaagacgg cctgccgcag
     1621 cgggcttgcc tggctctcca gggttgccca gttgccggtt tccaccctga ccatcgattg
     1681 acccgactca tacgccatga gcaggtcgcg cagggcgtcg ggtacgggtc tggaggtctg
     1741 ggtggcgggg tcggcaaaca catagatcag ctccccggtg accagcagcg tgtcgccccg
     1801 gaagatcgca ccatcaaaga tgatggaaga gttgccaata cggccacagc gtaggcctat
     1861 ttccagctgg tcgtcgtagc gcgcagagga aaaatactcc acactggctt tcttgacgta
     1921 gaggtccccg ttcaacatgt gcatggcagc ttcgtacggt acggcgagct cccgccagta
     1981 gtccgccatg gccgtgtcga aatacatgag gtagtgggcg ttgaacacga ttttctgcat
     2041 gtccacttca gcccaacgca cgcgcaggcg gtgcaggcag cggaaatcag cacgtttggg
     2101 attcatgtcg caaaagcctc cttgagggcc cgggccgcat cggcatgggc tttttccgct
     2161 tcggggatgg cgcgccccat cttgatgaac tcatggacca cgccacggta gatttccagg
     2221 tccacagcca cacccgccat gcgcagctgg tccgcgtaca gcacaccttc gtcgaccaaa
     2281 gggtcgcatt ctgccaagcc cagccaggca ggagcaacac cggagaggtc cgccacgtgc
     2341 ccggacggcg tgagcccatc cagtggtgca aaccgccagt cgtcgcggtc cgcctgggta
     2401 cgcacatagt ggttgaaaaa gtaggtgatg tggggttctt ccagcacaaa acctttgtca
     2461 aaagtgtggt gcgatggcgt gtcctggtga ccggcaaccc cagggt
//
LOCUS       DAOSJM010000361         2502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_36164_length_2502_cov_3.924806, whole genome shotgun sequence.
ACCESSION   DAOSJM010000361 DAOSJM010000000
VERSION     DAOSJM010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36164_length_2502_cov_3.924806"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2502
                     /locus_tag="PKX82_11275"
     CDS             <1..>2502
                     /locus_tag="PKX82_11275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOF52881.1"
                     /translation="LERAARSGEASLSGRMSLLRNDQLNTGFMYVLPVYRRGTDPATE
                     QQHMMALEGLLYAPMIAADAMGGVADVAMEAVQVELFDGDVRDNGNLLFGAHSQLVIH
                     QGDQPHAAGDARLYQTTRVMRIGGRLLNLRVGSTPAFAASLDRSAVTLMALSGTFGSF
                     MLALAVWLLASGRVRAQNLARRMTSELDTMARVVQTTDNAVVITDADLKITWVNDGFT
                     RMSGYTLAEALGHTPGELLGTSKSEPEVIARLEVAAAAGEACRVEVINRAKDGREYWI
                     DTDVHPVHNAYGVLTGFMEVGTDITSQKQAQLELQAAQSRMTELTNRLNLAIEGGSDG
                     LWDWMDLQQDAQWWSPSYYQLLGYTFEELPATGSNFASLVHPDHLHRIQEAMELALHH
                     GTTYDQEMLMRTKTHGFRWFRSRAKVFRDARGHAIRVSGSSQDIHDRKQAEAEMRQAE
                     ALLRGSIDALDDAYVLYDPQDRLVLCNQRHKDFYPLVADLFVPGNTFEFIIRTAMARG
                     QYPHAQGHEEAWIAERLVQHRLPQSNFQQVLDDGRILRISERRMPDGYTVGFRVDITD
                     FIRATEAAEAASRSKSQFLANMSHEIRTPMNAILGMLKLLQNTELTDRQQDYASKTEG
                     AARSLLGLLNDILDFSKVEAGKMTLDPRPFRLDKLLRDLSVILSSNVGSKDVEVLFDV
                     APDVPRALVGDDMRLQQVLINLGGNAIKFTSHGEVVLRLRVVEQTAGDVLLEFSVKDS
                     GIGIAPESQAHIFSGFSQAEASTTRRFGGTGLGLAISSRLDRLLGGDMQLVSAPGQGS
                     TFSFQIRLGLAPQEVQAPGIVPPQTHGQRTLVVDDN"
BASE COUNT          462 a          765 c          822 g          453 t
ORIGIN      
        1 cttgaacggg ccgcccgttc gggtgaggct tcgctctctg gtcggatgtc gttgctgcgc
       61 aatgatcagt tgaataccgg cttcatgtat gttcttccgg tgtaccgcag gggcaccgat
      121 cccgcgacgg agcagcagca catgatggcg ctggagggcc ttctgtatgc acccatgatt
      181 gcagcggatg ccatgggcgg tgtggccgat gtggccatgg aagcggttca ggtggagttg
      241 tttgatggcg atgtccgcga caacggtaac ctgctgttcg gagcccacag ccagctcgtg
      301 atccaccagg gtgatcagcc ccacgcggct ggcgatgcgc gcctgtacca gactacgcgt
      361 gtgatgcgca ttggcgggcg cctgctgaac ctgcgggtcg gcagcacacc ggcgtttgcc
      421 gcgtcgctgg accgatcggc ggtgacgctg atggcgctca gcggcacctt tggcagcttc
      481 atgctggcgc tggcggtctg gctgctggcg tcgggccgtg tgcgtgcaca gaacctggcg
      541 cggcgcatga cgtccgaact cgacaccatg gcccgcgtgg tccagaccac ggacaacgcg
      601 gtggtgatta ccgatgcaga tctgaagatc acctgggtca acgacggctt cacccgcatg
      661 agcggctata cgctggcaga agcgctgggc catacgccgg gggaattgct gggtaccagc
      721 aaaagcgagc ctgaagtcat tgcgcggctg gaggtggccg cggctgcggg cgaggcctgc
      781 cgggtggagg tgatcaaccg ggccaaggac ggccgcgagt actggatcga caccgatgtg
      841 cacccggtgc acaacgcgta cggtgtgctg accggcttca tggaggtggg caccgacatc
      901 acttcgcaaa agcaggccca gctggaactg caggcagcgc aatcccgcat gaccgagttg
      961 accaaccggc tcaatctggc gattgagggg ggtagtgatg gtttgtggga ctggatggac
     1021 ctgcagcagg acgcccaatg gtggtcgcca agctattacc agttgctggg atacacgttt
     1081 gaagagttgc ctgcaaccgg gtcgaatttt gcatccctgg tgcacccgga ccacttgcac
     1141 cggatccagg aggcgatgga actcgctctg caccacggca ccacctacga ccaggaaatg
     1201 ctgatgcgca ccaaaaccca cggcttccgc tggttccgct cgcgggccaa ggtgttccgt
     1261 gatgccagag gccatgcgat ccgtgtatcg ggttcttccc aggacatcca tgaccgcaaa
     1321 caggccgaag ccgaaatgcg ccaggccgaa gccctgctgc gcggctccat cgatgcgctg
     1381 gacgatgcct atgtcttgta cgacccgcag gacaggctgg tgctgtgcaa ccagcggcac
     1441 aaggattttt accctctggt ggctgacctg tttgtgccgg gcaacacttt cgaattcatc
     1501 atccggaccg ccatggcgcg cggccagtac ccgcatgccc agggccatga ggaagcctgg
     1561 attgcggaac gcctggtcca gcaccgtctg ccacagagca acttccagca ggtgctcgac
     1621 gacggccgca tcctgcgcat cagcgaacgc cgcatgcccg acggttacac cgtgggcttc
     1681 cgggtggaca tcaccgactt catccgtgcc accgaggcgg ccgaggcggc ctcgcgttcc
     1741 aagagccagt tcctggccaa tatgagccac gaaatccgca cccccatgaa cgcgattctg
     1801 ggcatgctca agcttctgca gaacaccgag ctcaccgacc ggcagcagga ctacgccagc
     1861 aagaccgaag gcgctgcgcg ctcgctgctg gggctgctca acgacatcct ggatttctcc
     1921 aaggtcgagg ccggcaagat gaccctggac ccacggcctt tccgtctgga caagctgttg
     1981 cgggacctgt cggtcatcct gtcctccaac gtgggcagca aggatgtgga agtgctgttc
     2041 gatgtggccc cagacgtacc gcgcgccctg gtgggcgacg acatgcggct gcagcaggtg
     2101 ctgatcaatc tgggcggcaa cgcgatcaag ttcacaagcc acggcgaagt ggtgctgcgc
     2161 ctgcgcgtgg tggagcagac cgccggggac gtgttgctgg agttctcggt caaggacagc
     2221 ggcatcggca ttgccccgga aagccaggcc catatcttca gcgggttttc gcaggccgaa
     2281 gcctccacca cccgtcgctt tggcggtacc ggtctggggc tggccatttc cagccgtctg
     2341 gacaggctgc tgggtggtga catgcagctg gtgagcgctc ccgggcaggg cagcacattc
     2401 tcgttccaga tccggctggg gctggcaccg caggaggtgc aagcgccagg gattgttccg
     2461 ccgcagaccc atgggcaacg tacgctggtc gtggacgaca ac
//
LOCUS       DAOSJM010000362         2480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_36586_length_2480_cov_3.665567, whole genome shotgun sequence.
ACCESSION   DAOSJM010000362 DAOSJM010000000
VERSION     DAOSJM010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36586_length_2480_cov_3.665567"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1355)
                     /locus_tag="PKX82_11280"
     CDS             complement(36..1355)
                     /locus_tag="PKX82_11280"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOF52882.1"
                     /translation="MSSVRFLTGITTSGTPHLGNYVGSIRPAVRASLTPGVESFYFLA
                     DYHALIKIDEPARIQRSTLEIAASWLAAGLNPDKVTFYRQSDVPEIPELTWFLTCVTG
                     KGVLNRAHAYKASVDKNTAAGVDPDADVTAGLFMYPVLMGADILMFNAHKVPVGRDQI
                     QHIEMARDMAASFNHRYGEHFVPPQAEIEESVATLPGLDGRKMSKSYDNTIPLFAPRE
                     QLKKLIAGIVTDSRAPGEPKDTEGSALFQIYQAFASEQETEALRTSFANGIAWGDAKN
                     VLFERIDREIAPMRAAYEDYMAHPEKVEALLLAGARKARAIATPFMAELRSAVGLRGL
                     GSAVQAGASKTAKVALASFKQYREKDGQFYFKLAAADGTVLLQSRAFESGKVAGQTVA
                     QLTRDGAAAIAALQAQLEPVDATGVAAAAAALADMAQAAASSDKKQA"
     gene            complement(1378..2046)
                     /locus_tag="PKX82_11285"
     CDS             complement(1378..2046)
                     /locus_tag="PKX82_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOF52883.1"
                     /translation="MDIAHLIQTVAIYALPVLFAITVHEAAHGYAARHFGDNTAWMLG
                     RVTLNPAKHIDPVGTILMPLLLYFVTSGAFLFGYAKPVPVRFDRLRNPKRDMVWVALA
                     GPAANLAQALAWGILVYLLPALGVTEPFFNRMCQGGVLVNVVMFAFNMFPLPPLDGGR
                     ILVGLLPHQQAALVSRIEPYGFFVVMALVVSGIASTVWMQPVMALTFGLLNLLLSPLA
                     ALVL"
     gene            complement(2077..>2480)
                     /locus_tag="PKX82_11290"
     CDS             complement(2077..>2480)
                     /locus_tag="PKX82_11290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOF52884.1"
                     /translation="LASYARVDNRQYRLLKLATPGPYTFILEASKEVPRRVSHPSRKT
                     IGLRVPEHKVLQELLALHGSALLATTLIAPGETDPLNDAHDIRDRYEHQIAAVLDSGA
                     CAQEPTTVIDLTGDEPEIIRQGRGDLARVGL"
BASE COUNT          460 a          711 c          836 g          473 t
ORIGIN      
        1 gtagctattg aattaatagc aaatgcattg cggttttatg cctgcttctt gtcactggat
       61 gccgctgcct gcgccatatc tgccagtgct gcggccgctg cggcaacgcc cgttgcgtcc
      121 accggttcca gttgcgcctg cagggcggca atggccgccg cgccatcgcg cgtgagttgc
      181 gccacggtct ggcctgctac cttgccggac tcaaaggccc tgctttgcag cagcacggtt
      241 ccgtcagcgg ctgcgagctt gaagtagaac tggccgtctt tttcgcggta ctgcttgaag
      301 gacgcgagcg ccaccttcgc ggtcttgctt gcgccagcct gcacggcgct gcccaggcca
      361 cgcaggccca cggcgctgcg caactcggcc atgaagggtg tggcaatggc gcgggctttc
      421 ctggcaccgg ccagaagcag ggcttccacc ttttctgggt gggccatgta gtcttcgtag
      481 gccgcgcgca tgggggcgat ttcccggtca atgcgttcga agaggacgtt cttggcgtca
      541 ccccaggcga tgccattggc gaaggatgta cgcagcgctt cggtttcttg ctccgacgca
      601 aaggcctggt agatctggaa caaggccgag ccctcggtgt ctttcggctc acccggcgcg
      661 cgcgagtctg tgacgatgcc agcaatcagc ttcttgagtt gctcgcgcgg agcgaagagt
      721 ggaatggtgt tgtcgtagct cttgctcatc ttgcggccgt ccaggccagg cagggtggcc
      781 acactctctt caatctcggc ttgcggaggc acgaagtgct cgccatagcg gtggttgaag
      841 ctggcggcca tgtcgcgggc catctcaatg tgctggatct ggtcgcgtcc cacgggcacc
      901 ttgtgcgcgt tgaacatcag gatgtccgca cccatcagca ccgggtacat gaacaggccc
      961 gctgtgacgt ccgcatcggg gtcgactccg gccgccgtgt tcttgtctac cgatgccttg
     1021 taggcgtggg cgcggttgag cacgcccttg ccggtgacgc aggtgaggaa ccaggtgagc
     1081 tccgggattt cgggcacatc ggactgccgg tagaaggtga ccttgtcggg gttgagccct
     1141 gcggccagcc agctcgcggc aatctccagc gtggagcgct ggatgcgtgc gggctcatcg
     1201 atcttgatca gggcgtggta gtccgccaga aaataaaaac tctcgacgcc cggggtcaga
     1261 ctggcgcgca ctgcggggcg gatggagccc acatagttgc ccaggtgggg tgtgccggag
     1321 gtggtgatgc cggtgaggaa gcgaacggaa ctcataacag gaacaatcag aaaaaaatca
     1381 gagaacgagt gcggccaggg gcgagagcag caggttcaga agaccaaagg tcagggccat
     1441 gacgggctgc atccacaccg tgctggcgat gccggacacg accagggcca tgacgacgaa
     1501 aaatccatag ggttctatcc gggacaccag tgccgcctgc tggtgcggta gcagccccac
     1561 cagaatgcgt ccgccatcga gggggggcaa cgggaacatg ttgaaagcaa acatcacgac
     1621 gttgaccagt acgccaccct ggcacatccg gttgaagaag ggttccgtca cgcccagggc
     1681 gggcagaagg tacaccagaa tgccccaggc cagtgcctgc gccagattcg cagccggtcc
     1741 tgccaaagcc acccacacca tgtcccgctt ggggttgcgc aagcggtcaa agcgaaccgg
     1801 aactggcttg gcatagccaa acaggaaagc cccggaggtg acaaagtaca gcagcagcgg
     1861 catgaggatc gtgccgaccg ggtcgatgtg cttcgcaggg ttgagggtga cccggcccag
     1921 catccatgcg gtgttgtcgc cgaagtggcg cgccgcgtat ccgtgcgccg cttcatgcac
     1981 cgtgatggcg aacagcacgg gcaaggcata gatggcaacg gtttgaatca ggtgagcaat
     2041 gtccacagga aggggtgcct tgttggttgg ggggtgttag agaccgaccc gggccaggtc
     2101 accgcgcccc tggcgaatga tttcgggttc gtcgcctgtg aggtcaatga cggtcgtggg
     2161 ttcctgggcg cacgccccgg aatcaaggac agcggcaatc tggtgttcgt accggtcgcg
     2221 gatgtcgtgt gcatcgttga gcgggtcggt ttcgcctggc gctattaaag ttgtagctaa
     2281 caaagcagac ccatggaggg ccaacagctc ctggagtact ttgtgctcgg gtacgcgcag
     2341 gccaatggtc ttgcgcgacg ggtggcttac gcggcgcggt acttccttgc tggcttccag
     2401 aatgaaggta taggggccgg gggtggcgag cttgagcagg cggtactgcc ggttgtccac
     2461 gcgcgcatag ctggccagct
//
LOCUS       DAOSJM010000363         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_36599_length_2479_cov_4.070957, whole genome shotgun sequence.
ACCESSION   DAOSJM010000363 DAOSJM010000000
VERSION     DAOSJM010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36599_length_2479_cov_4.070957"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PKX82_11295"
     CDS             complement(<1..1772)
                     /locus_tag="PKX82_11295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOF52885.1"
                     /translation="MVSLVSPPDVPDDSDRESASLSQFVHWIHGILFSAGLAVLVGIV
                     VADPQAWGRYSGLLVLLVVIALSHWQGRTHPRRSVAILVVGVWLLSCLSVLQFAGIHS
                     ASFVLFPFSIAVAGWMLGRRWLVATTLATFALALGVGLGELSGLFEPTPRAHPLIATM
                     QIAAIVLVVGFLVDAARTNLSRSRDRAVRLSSDLAAQVSEVATRERQWAMLLNNVPAA
                     IASFDAQSRLRNCNQRYADLFGTSTESIVGKTIQEYVPATALEQLREHWDKALAGEAQ
                     SYRRFNVHPQTHAVTWMDASVTPELEFGKVVGLYAVLVDVTDKVQAESEIRNLNADLE
                     GRVARRTEELAQAMDRLQESREALVSSQAKATLSALVASVSHELTTPIGNSVLVATTL
                     TDLSRQMQLQIDSGQVRKSSLLTLNRTLDEGSQLLLSNLARAETLLKNFKQVSVDQAS
                     EQRRTFVLDQMIGEVLSSLRPSLKLSPHRTVLDIPQGIAMDGLPGPLGQVVINLVNNA
                     YLHAFEGITAGVLTIRAADLGLRVEISIADNGVGMDPVVRAQVFNPFFSTKIGRGGTG
                     LGLSIVDSLVRKVLRGSLHIESSPG"
     gene            complement(1853..2341)
                     /locus_tag="PKX82_11300"
     CDS             complement(1853..2341)
                     /locus_tag="PKX82_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOF52886.1"
                     /translation="MQRPFQVLGIQQIAIGGPDKTRLQKLWVDMLGLEITGTFKSEKE
                     NVDEDICAIGKGPFKVEVDLMQPLDPEKKPAVHTTPLNHVGLWIDDLPVAVEWLTAKG
                     VRFAPGGIRKGAAGFDICFLHPKANDEFPIAGEGVLIELVQAPPEVVKAFSQMAFPGK
                     SV"
     gene            complement(2341..>2479)
                     /locus_tag="PKX82_11305"
     CDS             complement(2341..>2479)
                     /locus_tag="PKX82_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52887.1"
                     /translation="GERVAVIEAMKMENVLFAAADGVVGKVLATKGESLTVDQAIVEF
                     V"
BASE COUNT          508 a          807 c          703 g          461 t
ORIGIN      
        1 cccggactgg attcgatatg caacgaaccg cgcagaactt tgcggaccag ggaatccaca
       61 atgctcagac ccagacctgt accgccacgt ccaattttgg tgctgaagaa cggattgaac
      121 acctgcgccc gcaccaccgg atccatgccg acaccgttgt ctgcaatcga tatttctaca
      181 cgcaacccca ggtctgccgc gcggatcgtc aagacgccgg cggtgatgcc ttcgaaggca
      241 tgaaggtagg cgttgttgac caggttgatc accacctgcc ccagggggcc gggcagaccg
      301 tccatcgcga tgccctgcgg gatgtccagc acggtccggt gcggcgacag cttcaggctg
      361 ggccgcagcg acgacaggac ctctccaatc atctggtcca gcacaaacgt ccggcgttgc
      421 tcactggcct ggtccaccga cacctgcttg aagttcttca gtagtgtctc tgcgcgcgcc
      481 agattggaca gcagcaactg gctgccttcg tccagcgtgc ggttcagggt gagcaggctg
      541 gatttgcgca cctgcccgga atcgatctgc agctgcatct ggcgtgacag atcggtcagt
      601 gtggtggcca ccagcacgct gttgccgatg ggggtggtca gttcatgcga tacgctggcc
      661 accagcgccg acaacgtggc tttcgcctgg ctgctcacca gcgcctcgcg ggactcctgc
      721 agccggtcca tcgcctgcgc gagttcttcc gtgcggcgcg cgacgcgtcc ttcgagatct
      781 gcattgaggt ttctgatctc ggattcggcc tgcaccttgt cggtcacgtc taccaacacc
      841 gcgtacaggc cgaccacctt gccgaactcc agctcggggg tcacgctggc atccatccag
      901 gtcaccgcat gggtttgcgg atggacattg aaacggcgat aactctgcgc ctcgcccgcc
      961 agcgccttgt cccagtgctc gcgcagttgt tccagtgcag ttgcgggaac gtattcctgg
     1021 atggtcttgc caacgatgga ttcggtggat gttccaaaca agtccgcgta acgctggttg
     1081 caattgcgca gccgggactg tgcgtcaaat gacgcaatgg cagcaggcac attgttcagc
     1141 agcatggccc attgccgttc acgcgttgcg acttcggaca cctgcgccgc cagatcactg
     1201 gacaaccgga ctgcacgatc ccggctcctg ctcaggttgg tacgggcagc atccaccagg
     1261 aacccgacca ccagcacaat ggctgcaatc tgcatggttg caatcagtgg gtgcgcccgt
     1321 ggggtgggct caaacaatcc ggagagttcg cccaacccca cacccaaggc cagggcaaaa
     1381 gttgccagcg tggtagcaac cagccagcgc cggcccaaca tccagccggc aaccgcaata
     1441 gaaaagggaa agagtacgaa gctggcactg tggatgccgg caaactgcag gaccgacagg
     1501 caggacagga gccacacgcc aaccaccaga atggccaccg atcggcgcgg gtgggtgcga
     1561 ccttgccaat gactcagggc gatcaccacc aacagaacca gcaggccgct gtaccgtccc
     1621 caggcctgcg gatctgcaac cacaataccg accaacaccg ccaagcctgc cgaaaacaga
     1681 atgccatgaa tccagtggac aaactgggaa agcgatgctg actcgcgatc cgagtcgtcc
     1741 ggaacgtcag gcggcgaaac gagcgaaacc atggtccaac attggcgctg tgtatttcag
     1801 tgcactatac gccggcaaag gcctgcagtc gcacgggccg gctggccatg actcagacgc
     1861 tcttaccggg aaaggccatt tgtgaaaatg ctttcaccac ttccggcggt gcctgcacca
     1921 gttcaatcag cacaccttcg ccagcgatgg ggaattcgtc gttagccttg gggtgcagaa
     1981 agcagatgtc aaagcctgca gcgcctttgc gaatgccgcc aggcgcaaaa cgcacgcctt
     2041 tggcggtcag ccactccacc gccaccggca agtcgtcaat ccacagaccg acgtggttca
     2101 gtggggtggt gtgcactgcc ggcttctttt cggggtccag cggctgcatg aggtcgacct
     2161 cgaccttgaa cgggcccttg ccaatggcgc agatgtcctc gtccacgttc tctttttcgc
     2221 tcttgaacgt accagtgatc tccagaccca gcatatccac ccacagcttt tgcaggcggg
     2281 tcttgtcggg gccaccgatg gcgatttgct ggatgcccag cacctggaat gggcgctgca
     2341 tcacacaaac tccacgatcg cctggtccac cgtcaggctc tcgcccttgg ttgccagcac
     2401 cttgcccacc acgccatccg ccgcagcaaa cagcacgttc tccatcttca tggcttcaat
     2461 cacggccacc cgctctccc
//
LOCUS       DAOSJM010000364         2461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_36952_length_2461_cov_3.867830, whole genome shotgun sequence.
ACCESSION   DAOSJM010000364 DAOSJM010000000
VERSION     DAOSJM010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36952_length_2461_cov_3.867830"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="PKX82_11310"
     CDS             complement(<1..225)
                     /locus_tag="PKX82_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52888.1"
                     /translation="MRKLFTFLCLCLIGGWVYAAAISQTDMLPGSAQAQPAWSQAPCH
                     EHTAPALASHASPCKSDVHTCCHGAFFFPTL"
     gene            complement(324..522)
                     /locus_tag="PKX82_11315"
                     /pseudo
     CDS             complement(324..522)
                     /locus_tag="PKX82_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425171.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1234)
                     /locus_tag="PKX82_11320"
     CDS             complement(614..1234)
                     /locus_tag="PKX82_11320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOF52889.1"
                     /translation="MQSKAHWEKVYTTKAPDNVSWFQAHAETSVRMIHDTGLGREASI
                     IDVGGGASTLVDDLLDDGYRAITVLDLSGAALDESKRRLGTKGKHVHWMETDITRAVF
                     EPHSCDIWHDRAVFHFLTTDEDRRAYVKQVLHALKPGGHVIMATFGTHGPAQCSGLPV
                     VRYAPEELHSEFGEAFTLLTHEEELHHTPFGTDQQFIYCMCRKTAT"
     gene            complement(1255..1686)
                     /gene="cadR"
                     /locus_tag="PKX82_11325"
     CDS             complement(1255..1686)
                     /gene="cadR"
                     /locus_tag="PKX82_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Pb(II)-responsive transcriptional
                     regulator"
                     /protein_id="HOF52890.1"
                     /translation="MKIGELAQVAQTTVETVRYYEKEGLLPETTRTAGNFRVYGPTHL
                     ERLRFIRNCRALDMSHGEIHILLGLADQPDEGCGAINAVFDQHMAHVDERIRELVHLK
                     EQLALLRQRCQTERAVDACGILQGLAAMATEAKPERHTHLG"
     gene            1764..>2461
                     /locus_tag="PKX82_11330"
     CDS             1764..>2461
                     /locus_tag="PKX82_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012492246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52891.1"
                     /translation="MTAPEHHDHAPTHDPGPNACCGGCTAGVAMPVPLARSKEVVAGS
                     GVQTPIRILQMDCPTEEALLRTKLGGMAGVAGMEFNLMQRVLTVTHEPQALEPVLAAV
                     RSLGFTPEIAGDAQAEPAPEPVRPWWPLAVAGLAAIGSEASHWLGFPDALAAALALIA
                     VLACGISTYKKGWMAVRNGNLNINALMSIAVTGALVLGQWPEAAMVMVLFTVAEWIEA
                     RSLDRARNAIRSLM"
BASE COUNT          476 a          751 c          730 g          504 t
ORIGIN      
        1 cagtgtggga aagaagaacg caccgtggca gcaggtgtgc acatccgatt tgcatggaga
       61 tgcgtgcgat gccagtgccg gagccgtgtg ttcatggcaa ggggcctgtg accacgcagg
      121 ctgcgcttgc gcgctgccgg gcagcatatc cgtctgggaa atggcagccg catacaccca
      181 gccgccgatc aggcagaggc agaggaaggt gaagagtttg cgcatccagt tggttccaaa
      241 cacgctcaat gtgcgtcaag tggaaccagc aagccttgac gtaaagcaag aaatccgtga
      301 aacgccgagc gcaaacgcca ggcagtgttg acgcagccgg ccaccagtcc tttgctcccc
      361 gcgatagaaa gtgcatctga tgccgtcact ttgcgcgtga gatggttatc aatggccaaa
      421 gccagagggc ccatgtatcg ccgttaacac cgactccagg tccagaaaca gcggccccaa
      481 caacccgcca aacgcaatcg cccctatcag ccagggccac tcgcgtcccg gcatttccgt
      541 cgacttctac ccgtggcccc ttgctaaccg ggtcagtgtc aaacccaagc cactgccgac
      601 agatgcaacc gactcatgtc gccgtctttc tgcacatgca atagatgaac tgctggtcgg
      661 ttccgaaagg cgtgtggtgc aactcttcct catgggtcaa caaggtaaac gcctcgccaa
      721 attcagagtg cagctcttcc ggtgcgtagc gcacgaccgg tagcccactg cactgtgccg
      781 ggccgtgggt gccgaaggtg gccatgatga cgtgtccacc gggtttcagt gcgtgcagca
      841 cctgcttcac atacgccctg cggtcttcgt ccgtggtcag gaagtgaaac acggcacgat
      901 cgtgccagat gtcacagcta tgaggttcaa acaccgcacg ggtgatgtcc gtttccatcc
      961 agtgtacgtg cttgcctttg gtgcccagac ggcgcttgct ctcgtccagt gctgctccag
     1021 acaaatccag caccgtaatg gcacggtagc cgtcgtccag cagatcatcg accagcgtgg
     1081 atgcaccgcc gcccacatcg atgatggaag cttcgcgccc cagacccgta tcgtgaatca
     1141 tgcgcaccga cgtttcggcg tgcgcctgaa accagctcac gttgtccggt gctttggtgg
     1201 tgtacacctt ttcccaatgc gctttgcttt gcatcgttat ttcctttttt tcatttaacc
     1261 cagatgggtg tggcgttctg gcttggcttc ggttgccatg gcagccaggc cctgcaaaat
     1321 accgcaggca tccaccgccc gctcggtctg gcagcgttgg cgcaataggg ccagttgctc
     1381 cttcaagtgc accagctcac ggatgcgctc atccacatgg gccatgtgct ggtcaaatac
     1441 ggcgttgatg gcaccacagc cttcgtcggg ctggtcggcc agacccagca agatgtggat
     1501 ttccccgtgg ctcatgtcca gtgcgcggca gttgcggatg aaacgcaagc gctccagatg
     1561 ggtcggacca tagacccgga agttgcctgc tgtacgggta gtctcgggta gtagaccttc
     1621 cttctcgtag tagcgcacgg tttccaccgt ggtctgggcg acctgggcca attcaccgat
     1681 tttcatggcg ggttccaaat agttcttgac tctatagtag cttcaggttg cgtaataggg
     1741 ccagtttcca tgtaaagacc cttatgacag cacccgagca ccacgaccac gcccctaccc
     1801 acgaccctgg cccgaatgcc tgttgcggtg gatgcaccgc cggcgtggcc atgccggtac
     1861 ccctggcgcg ctccaaggaa gtggtagcgg gttcgggcgt gcagacgcct atccgcatcc
     1921 tgcagatgga ctgccctacg gaagaagcgc tgttgcgcac aaagctcggt ggcatggcgg
     1981 gtgtggccgg catggagttc aacctgatgc agcgggtgct caccgtcacc catgaacccc
     2041 aggcgctgga gccggttctc gcggctgtgc gctccctggg ttttacacct gaaattgcgg
     2101 gtgatgcgca agctgaaccg gcaccggaac cggtcaggcc ctggtggccg ctggccgttg
     2161 cgggtcttgc agccatcggc tccgaggcat cccactggct gggtttcccg gatgccctgg
     2221 ccgccgcgct ggcgctgata gccgtgctgg cctgcggcat cagcacctac aagaagggct
     2281 ggatggccgt ccgcaatggc aacctgaata tcaacgccct gatgagcatt gccgtcaccg
     2341 gtgctctggt actggggcaa tggccggaag cggccatggt gatggtcctg ttcaccgtgg
     2401 ccgagtggat tgaagcccgg tcattggacc gtgcgcgcaa tgccattcgc agcctgatgc
     2461 a
//
LOCUS       DAOSJM010000365         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_37268_length_2444_cov_5.240686, whole genome shotgun sequence.
ACCESSION   DAOSJM010000365 DAOSJM010000000
VERSION     DAOSJM010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37268_length_2444_cov_5.240686"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKX82_11335"
     CDS             <1..396
                     /locus_tag="PKX82_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOF52892.1"
                     /translation="TLIERVGICRDFAHLMIALCRALNIPARFTTGTDYGADPILGPP
                     DFHAYVEAYLGDRWYIFDPSGTAMPMGLVRFGTGRDAADVAFATIFGGVQSHAPVIHA
                     WAVEDASQGIVLPYHCRDAMSTDGGHQLL"
     gene            complement(430..1062)
                     /locus_tag="PKX82_11340"
     CDS             complement(430..1062)
                     /locus_tag="PKX82_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOF52893.1"
                     /translation="MTWLSPEQFFGFLSAALLITLSPGPDNLMVLSIGASRGRRLGMA
                     FGLGCGLGCLSHTVLAALGVGALIAASPAAFLALKLAGGAYLMVLGWQALRSSGRMNL
                     QADGAVEVSVWQLFGKGMFANAINPKVVMFFLAFLPQFVVSGRGDAPWQIVQLGVLFT
                     LQAAVLFGALGYFAGHVGRWLHRNASAGLWLDRIAGCVFVGLGFKLIMGL"
     gene            complement(1059..1487)
                     /locus_tag="PKX82_11345"
     CDS             complement(1059..1487)
                     /locus_tag="PKX82_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOF52894.1"
                     /translation="MNAIVLDTNIVLDLCVFRDPAVEPLRHSLDQRVLQWIATPPMRE
                     ELARVLAYPQIIKSLNHHQTRAADVLARFDAQVCMVEVAAKAAFTCKDADDQKFIDLA
                     VAHRAALVSKDHAVLCMARRLRTLGVAVQAVFSPEMLVPA"
     gene            complement(1484..>2444)
                     /locus_tag="PKX82_11350"
     CDS             complement(1484..>2444)
                     /locus_tag="PKX82_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOF52895.1"
                     /translation="TAQHSPLNSLNFAALKIKDAVCDRFRAKAGGIRPDVNTQWPDVR
                     IYAHLTTDTVSLYIDTSGEALFKRGWREDKGEAPLKETLAAAMIAASGWADGEDDLQP
                     LYDPCCGSGTIAIEAAQIACNIAPGSLRRFAFEKYVPFQPHVWAAIKQEAADAVVKPA
                     PGQKALIYGSDVSHRMVDFAQRNAERAGVADVIEFRGGDALQRMPPIEGGGVMVLNPP
                     YGERIGIGGVAGEGVQARFGQREIAQTEDGGEFFSQLATHWKKNFSGWTAWVLTPDLK
                     LPGKMRLKESRRVPMWNGPLECRLFKFDMVAGKMAPKAGKGAP"
BASE COUNT          453 a          848 c          736 g          407 t
ORIGIN      
        1 acgctgatcg agcgcgtggg catctgccgc gacttcgccc acctgatgat tgcactgtgc
       61 cgcgcgctca acattccggc gcgcttcacc accggcaccg actatggtgc cgacccgatt
      121 ctgggtccac ccgactttca tgcgtatgtc gaggcgtatc tgggcgaccg ctggtacatc
      181 ttcgacccct cagggaccgc catgcccatg ggcctggtgc gctttggcac cgggcgcgat
      241 gcggccgatg tggccttcgc caccatcttc ggtggcgtgc agtcgcacgc accggtcatc
      301 cacgcctggg cggtggagga tgccagccag ggcatcgtgc tgccgtacca ctgcagggac
      361 gccatgtcca ccgacggcgg gcatcaattg ctataaagtt aatagctgac tgcgcaatga
      421 atacgggggc tagagcccca taatgagctt aaagcccagc cccacgaaga cgcaccccgc
      481 gatccggtcc agccacaggc ctgcactggc gttgcggtgg agccaccggc ctacgtggcc
      541 cgcaaagtag cccagggcac caaagagcac cgccgcctgc agcgtgaaga gcacccccag
      601 ctggacgatc tgccagggtg catcaccgcg cccgctgacc acaaactgcg gcaggaacgc
      661 cagaaagaac atcaccacct tggggttgat ggcattggca aacatgcctt tgccaaacag
      721 ctgccacaca gacacttcca ccgcgccatc ggcctgcaga ttcatacgcc ccgaactgcg
      781 caaggcctgc cagcccagga ccatgaggta ggcaccgcca gccagcttga gcgccagaaa
      841 tgccgcgggc gacgccgcaa tcagcgcgcc cacacccagg gccgccagca ccgtgtggct
      901 cagacacccc agtccgcagc ccaggccaaa cgccatgccc agccgtcgcc cacgcgaggc
      961 cccgatggac agcaccatca ggttgtctgg ccctggtgac agggtgatca gcagtgccgc
     1021 actgagaaat ccgaagaact gctccggcga cagccaggtc atgcgggcac caacatctct
     1081 ggcgaaaaca ccgcctgtac cgcgaccccc agcgtgcgca ggcgccgggc catgcacagc
     1141 accgcatggt ccttgctcac cagcgccgca cggtgggcca ccgccaggtc aatgaacttc
     1201 tggtcgtcgg cgtccttgca ggtgaatgcg gccttggctg ccacctccac catgcacacc
     1261 tgcgcatcga accgcgccag tacatccgct gcgcgggtct ggtggtggtt caggcttttg
     1321 atgatctggg gataggccag cacacgtgca agctcctcgc gcatgggcgg cgtggcaatc
     1381 cactgcaata cccgctggtc cagggagtgg cgcaggggct ccacggccgg gtcacgaaaa
     1441 acacacaggt ccaggacgat gttggtgtcc agaacgatgg cgttcaaggc gcacctttgc
     1501 ccgctttggg cgccatcttt ccggccacca tgtcgaactt gaacagccgg cattccagcg
     1561 ggccgttcca catcggtacg cggcgcgact ccttgaggcg catcttgccg ggcaacttga
     1621 gatcgggcgt cagcacccag gccgtccagc ccgagaagtt ctttttccag tgcgtggcca
     1681 gctgactgaa aaattcgccg ccatcttcgg tctgggcaat ctcgcgctgg ccaaaacggg
     1741 cttgcacgcc ctcccctgca acaccgccaa tgccgatgcg ctcgccatac ggcgggttca
     1801 gcaccatcac gccgccgccc tcgatgggcg gcatgcgctg cagcgcgtcg ccaccgcgga
     1861 actcgatcac atcggccaca ccggcgcgct cggcattgcg ctgcgcaaag tccaccatgc
     1921 ggtgcgacac atcgctgccg taaatcaggg ctttctggcc cggcgcaggc ttgaccactg
     1981 cgtctgccgc ttcctgcttg atggccgccc acacgtgggg ttgaaacggc acgtatttct
     2041 caaaggcgaa ccggcgcagc gaacccggtg caatattgca ggcaatctgt gccgcctcaa
     2101 tggcaatcgt gccgctgccg cagcacgggt catacagggg ttgcaggtca tcctcgccat
     2161 cggcccagcc gctggcggca atcatggcgg cggccagggt ttccttcagc ggcgcctcgc
     2221 ccttgtcctc gcgccagccg cgcttgaaca gcgcctcgcc cgaggtgtcg atatacagcg
     2281 acaccgtgtc cgtggtcaga tgggcataga tgcgcacgtc gggccactgg gtgttcacgt
     2341 ccggccggat gccgccggcc ttggcgcgga agcggtcgca gaccgcatcc ttgattttga
     2401 gcgcggcaaa gttcaggctg ttgagcgggc tgtgctgggc cgtc
//
LOCUS       DAOSJM010000366         2441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_37331_length_2441_cov_3.850796, whole genome shotgun sequence.
ACCESSION   DAOSJM010000366 DAOSJM010000000
VERSION     DAOSJM010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37331_length_2441_cov_3.850796"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PKX82_11355"
     CDS             complement(<1..1116)
                     /locus_tag="PKX82_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOF52896.1"
                     /translation="MSLALWTLVGVLVAGMLLRMPIGFSMLASGVGYLLVKRQDLGLV
                     AEQVSNGLYNSYVLLAVPLFVFAANIMNAGTVSERIFDFCRILVGRMRGGLAQVDILV
                     SVIFSGMSGSAIADAAGPGLVTIKQMLKKPEYTRGFAGAVVVASATLGPIIPPSIPMV
                     IYALVSGTSVGALFLGGVLPGLLMAALMMLVVHIIAVKRNMPRDEPVPRSEWAGLIFR
                     GALPLSMPLVLLGGIYSGAFTPTEAAAVAALHSLILAAVVFRALTWRSFWGVVMESTR
                     GSAVITLILAGSFMLNYAFTAEGVPQAMAQWVDSMQLSQIKFLLLVNVMFLVLGCFLD
                     VSVLLLVFVPMLLPAARLLGVDMVHFGVLVVLIMMIGL"
     gene            complement(1113..1595)
                     /locus_tag="PKX82_11360"
     CDS             complement(1113..1595)
                     /locus_tag="PKX82_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOF52897.1"
                     /translation="MARWLQRAANLVGGGLFLTLFIVFIIQITARFGFNKPLPWTDEA
                     AVILYIWVILWAAAVVVPEREHVVFDLVWNSAGRRTRQAMRIAGNLLIGGLCAVALPA
                     SWDYVHFMQREGTPVLNMPLMWVYLPFVILMAALVVRSAWAIWDAARGRGLEAELHLQ
                     "
     gene            complement(1596..>2441)
                     /locus_tag="PKX82_11365"
     CDS             complement(1596..>2441)
                     /locus_tag="PKX82_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HOF52898.1"
                     /translation="KDTLEKSAPGEFDVQINLNASLFKQGTEPAAMARGNLELTTVSA
                     SDIAKLVPEFSIFTAGYVVRDPAQQQKIFNGPIGAELFKTVAEKMDITPLSTCYLGTR
                     QVNLREVRNVKTPADLKGVKLRMPGSKDWLFLGEALGATATPLAFGEVYLGLKTGTID
                     GQDNPLPTVRAAKFYEVTKQIVMTSHLVDGLFIAISNKSMNALNAAQKAKVLAAAQAA
                     AAYNNENRLKEEAQLVEFFKKEGLQVTVPNVDAFHKSVQATYMASDYAKVWPKGLLDR
                     INATK"
BASE COUNT          496 a          820 c          725 g          400 t
ORIGIN      
        1 taggccgatc atcatgatca gcaccaccag caccccaaag tgcaccatgt ccacgcccag
       61 caggcgcgcc gccggcaaca gcatgggcac gaacaccagc agcagcaccg acacatccag
      121 aaaacagccc agcaccagga acatcacgtt cactaacagc agaaacttga tctgactgag
      181 ttgcatgctg tccacccatt gcgccatggc ctggggtact ccctcggcag tgaacgcgta
      241 gttcagcatg aacgaaccgg ccagaatcag cgtgatgacc gcactgccgc gggtggactc
      301 catcaccaca ccccagaaac tgcgccaggt cagtgcccgg aacaccactg ccgccagaat
      361 cagcgaatgc agcgccgcca ccgcagccgc ctcggtgggc gtgaacgcgc ccgagtaaat
      421 gccgcccagc agcacgaggg gcatggacag cggcagggca ccacgaaaga tcagccccgc
      481 ccactcggag cggggcaccg gttcgtcgcg cggcatgttg cgcttgacgg cgatgatgtg
      541 caccaccagc atcatcagcg cggccatcag caggcccggc agcacgccgc ccaggaacag
      601 cgcacccacc gatgtacccg acaccagcgc atagatcacc atcggaatgc tcggcggaat
      661 gatgggcccc agcgtggcgc tggccaccac caccgcgccg gcaaagccac gcgtgtactc
      721 cggcttcttg agcatctgct tgatggtgac caggcccggg ccggcggcgt cggcaatggc
      781 gctgccgctc atgcccgaga agatgacact gaccagaatg tccacctgcg ccagaccgcc
      841 gcgcatgcgc cccaccagaa tgcgacagaa atcaaaaatg cgctcgctca ccgtgcccgc
      901 attcatgatg ttggcggcaa acacaaacag cggcaccgcc agcagcacat agctgttgta
      961 caggccgttg ctcacctgct cggctaccag accgaggtcc tgccgtttga ccagcaggta
     1021 gcccacgccc gaggccagca tcgaaaaacc aatcggcatg cgcagcagca tgcctgctac
     1081 caatacgccc accagggtcc agagtgcgag gctcattgca ggtgcagctc cgcttccagg
     1141 ccacgccccc gcgcggcgtc ccagatggcc caggcgctgc gcaccaccag cgccgccatc
     1201 aggatcacaa agggcaggta cacccacatc agcggcatgt tcagcaccgg cgtgccttcg
     1261 cgctgcatga agtgcacata gtcccagctg gccggcagcg ccacggcaca caagccgcca
     1321 atcagcagat tgccggcaat gcgcatggcc tggcgcgtgc ggcggcctgc gctgttccac
     1381 accaggtcaa acaccacatg ctcgcgctcg ggcaccacca cggcggccgc ccacaggatg
     1441 acccagatgt agagaatcac cgccgcctca tccgtccacg gcaatggctt gttgaagcca
     1501 aagcgcgccg tgatctggat gatgaagacg ataaacagcg tcaggaacaa gccgccacca
     1561 accaggttgg cagcgcgctg cagccagcgg gccatttact tggtggcgtt gatgcggtcc
     1621 agcaggccct tgggccagac cttggcgtag tccgaggcca tgtaggtagc ctgcaccgat
     1681 ttgtggaagg catccacatt gggcactgtg acctgcaggc cttccttctt gaagaactcc
     1741 accagctggg cttcttcctt gaggcggttc tcgttgttgt aggccgctgc ggcctgggcg
     1801 gcggcgagta ccttggcttt ttgcgcggca ttgagcgcgt tcatgctctt gttggagatg
     1861 gcaatgaaca gtccgtccac caggtggctg gtcatcacga tctgtttggt cacctcgtag
     1921 aacttggccg cacgcaccgt gggcaggggg ttgtcctggc cgtcgatggt gccggttttc
     1981 aggcccaggt acacctcgcc aaaggccagc ggcgtggcgg tggcgcccag cgcctcgccc
     2041 agaaacagcc agtccttgga gccgggcatg cgcagcttca cgcccttgag gtcggcggga
     2101 gtcttgacgt tgcgcacttc gcgtaggttg acctggcgcg tgcccaggta gcaggtggac
     2161 aggggggtga tgtccatctt ttcggccacc gtcttgaaca gctcggcgcc aatggggcca
     2221 ttgaaaatct tttgctgttg cgccgggtcg cgcaccacat agcccgcggt gaagatggaa
     2281 aactccggca ccagcttggc aatgtccgac gccgacaccg tggtcagctc cagattgccg
     2341 cgcgccatgg cggccggctc ggtgccctgt ttgaacagcg aggcgttgag gttgatctgc
     2401 acatcaaact cgcccggtgc gcttttttcc agcgtgtcct t
//
LOCUS       DAOSJM010000367         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_38335_length_2392_cov_3.554129, whole genome shotgun sequence.
ACCESSION   DAOSJM010000367 DAOSJM010000000
VERSION     DAOSJM010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38335_length_2392_cov_3.554129"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="PKX82_11370"
     CDS             complement(<1..885)
                     /locus_tag="PKX82_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOF52899.1"
                     /translation="MRSTVWTRWFPFLAWPRPDLTLLRGETLAAFTVAILLIPQAVAY
                     AALAGMPLVSGLYASLLPALISSLWGGTSRLSVGPTALTCLLIAASLQGMAQPASAEW
                     VALAVWLALFSGAIQLVLGLLRAGWLVNMISAPVLAGFTQAAGVLIIGSQLPALLGMQ
                     GPLSSVLEQPHFHLGALAFGLGTVLLLWLAKRYFPRWPTVLGVVAVSGVVSYATGFEN
                     AGGAVVGALPVGLPDLYFPPLLPWSVLSQLIVPSMVIALVSFLEVASSAKVQSQQDGS
                     LWNDNQDLVAQGLAKIASA"
     gene            complement(882..1649)
                     /gene="moeB"
                     /locus_tag="PKX82_11375"
     CDS             complement(882..1649)
                     /gene="moeB"
                     /locus_tag="PKX82_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HOF52900.1"
                     /translation="MQDDELLRYSRHILLDDIGIEGQTRILQGHALVIGAGGLGSPVA
                     MYLAVAGVGHLTLVDHDTVDLTNLQRQIAHHSDSVGQPKVQSASRTVHALNDRVQVTA
                     LQERADAALLDTLVPQADVVLDCCDNFTTRQAVNAACVRHGVPLVSGAAIQFDGQISV
                     YDPRNAASPCYACAFPPDVTFEEARCATMGVFAPLVGIIGSMQAAEALKLLAGVGQSL
                     AGRLLMLDGRAMEWNEVRIPRNTNCPVCSSHIGTKQP"
     gene            complement(1657..>2392)
                     /locus_tag="PKX82_11380"
     CDS             complement(1657..>2392)
                     /locus_tag="PKX82_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOF52901.1"
                     /translation="DLRNDPGGLLNAAVAISATFLPENVTVVSTNGQTPESKQTFKAS
                     VRDYMSRAGADPLKTMPAGLKNVPLVVLVNEGSASASEIVAGALQDHKRATIMGSQTF
                     GKGSVQTFRPLGPDTGLKITTSRYYTPSGKSIQAKGIVPDVMLDDTEEGSPYAALRMR
                     EADLEKHLGSGQGEEVKDAAREKAREEALKRLEEESRKPASERRAPEFGTEKDFQLQQ
                     ALNQLKGKPVLVSKTLVERKEEKKDN"
BASE COUNT          439 a          737 c          769 g          447 t
ORIGIN      
        1 tgcggaggca atttttgcaa gaccctgtgc gaccagatcc tggttgtcat tccacagact
       61 gccatcctgc tggctctgca ccttggcact ggacgccact tccagaaagc tcaccagtgc
      121 aatcaccatg gacggaacaa tcagctggct caagaccgac cagggcagca ggggggggaa
      181 atacaggtct ggcagaccga cagggagcgc gcccaccacc gcacctccgg cgttctcaaa
      241 tccggtggcg taactgacca cacctgacac agccacgacc ccgagtaccg tgggccagcg
      301 tgggaaatag cgtttggcca gccagagcaa gagtacggtt cccagaccaa atgccaaagc
      361 accgaggtgg aagtggggtt gctccagcac agaggagagc ggcccctgca tgcccagcaa
      421 agcaggaagt tgcgagccga tgatgagaac cccggcggcc tgtgtgaacc ctgcgagtac
      481 tggcgcactg atcatgttga ccagccaccc ggcacgcaac aggcccagca ccagttgaat
      541 ggcaccggaa aacagtgcca gccacacggc cagtgcgacc cattcggcac tggcgggttg
      601 tgccatgccc tggagtgacg ctgctatcag cagacaggtc agtgcggtgg ggcccaccga
      661 caaccggctg gtacctcccc acaggctgga gatgagtgcc ggaagcaggg atgcatacag
      721 gccactcacc aggggcatgc cagccagcgc cgcataggcg accgcctgcg gtatcaggag
      781 aatggcgacg gtaaaggccg caagggtctc tccgcgcagt agcgtgaggt ccggtctggg
      841 ccaggcaaga aaaggaaacc agcgggtcca gacagtggat ctcacggttg cttggtgcca
      901 atatgactgg aacagaccgg gcagttggtg ttgcgtggta tgcgcacttc gttccattcc
      961 atggcgcgtc catcgagcat cagcaggcgc ccggccaggg actggcccac gccggccagc
     1021 agcttgaggg cttccgccgc ctgcatggaa ccaatgatgc ccaccagcgg cgcaaagacg
     1081 cccatggtcg cgcaacgtgc ttcctcgaac gtcacgtcgg gcgggaatgc gcacgcgtag
     1141 cagggcgaag ccgcgttacg tgggtcgtag acgctgatct gtccatcgaa ctggatggct
     1201 gcaccggata ccagtggcac gccatggcgc acgcaggctg cgttgaccgc ctggcgggtg
     1261 gtgaagttgt cgcagcagtc cagcaccacg tcggcctggg gcaccagcgt atccagcaga
     1321 gccgcatcgg cacgctcctg cagggccgtg acctggacgc ggtcgttgag ggcatggacg
     1381 gtgcgactgg cggattgcac cttgggttgc cccacgctgt cgctgtggtg cgcgatctgg
     1441 cgctgcaggt tggtgaggtc cacggtgtcg tggtccacca gcgtgaggtg tcctacgccg
     1501 gccaccgcca ggtacatggc caccggggag cccaaccccc cggcaccgat gaccagcgca
     1561 tggccctgaa ggatgcgggt ttgtccttca atgccaatgt cgtccagcag gatgtggcgc
     1621 gagtagcgca gcagctcgtc gtcctgcatg gtgcggctag ttgtcttttt tctcttcctt
     1681 gcgttccaca agggtcttgc tcaccagcac cggcttgcct ttgagctggt tcagcgcttg
     1741 ctgcaactgg aagtcctttt cggtgccgaa ttcaggcgca cggcgctcgg acgctggctt
     1801 gcgcgattct tcttccaggc gcttgagggc ttcttcgcgg gccttctcgc gggcggcgtc
     1861 tttcacctct tcaccctggc cgctgcccag atgtttttcc agatcggctt cccgcatgcg
     1921 cagtgcggcg taggggctgc cttcttcagt gtcatcaagc atcacgtcgg gcacgatgcc
     1981 cttggcctgg atggacttgc cgctgggggt gtagtagcgc gaggtggtga tcttcaggcc
     2041 ggtatccgga cccagcggac ggaaagtctg caccgatccc ttgccaaacg tctggctgcc
     2101 catgatggtg gcgcgtttgt ggtcttgcag tgcaccggcc acgatttcgc tcgcggaggc
     2161 cgatccttcg ttgaccagca ccaccagcgg cacatttttc aggcctgcag gcatggtctt
     2221 gagcgggtca gcgcctgcgc ggctcatgta gtcacgcacc gatgccttga aggtctgttt
     2281 gctctcgggt gtctggccgt tggtggacac cacggtcacg ttctctggca ggaaggtggc
     2341 cgagatcgca accgctgcat tgagcaggcc acctgggtca ttgcgcagat cc
//
LOCUS       DAOSJM010000368         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_38581_length_2378_cov_3.685321, whole genome shotgun sequence.
ACCESSION   DAOSJM010000368 DAOSJM010000000
VERSION     DAOSJM010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38581_length_2378_cov_3.685321"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..2188
                     /locus_tag="PKX82_11385"
     CDS             104..2188
                     /locus_tag="PKX82_11385"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOF52902.1"
                     /translation="MPTSTAAEADPFQALNVEQRQAVEHGLGNESADTRPLLIIAGAG
                     SGKTNTLAYRVANLIVHQADPQRMLLLTFSRRAAQEMEHRVGAVLSRVLGQSSALQLP
                     SLPWAGTFHSIAARLLRCYAGRIGLDDSFTILDRGDAEDLMGMVRHDLGFSSTQRRFP
                     QKGTCLSIYSRVVNSQEPLLQIMQQVFPWCVQWQSELKSLFGCYVDAKQAQNVVDYDD
                     LLLFWSEMAAEPQLAAELGERFDYVLVDEYQDTNRLQASILLGIKPSGKGVTVVGDDA
                     QSIYSFRGATVRNILDFPRQFGQPSRLVTLERNYRCTQSILDVSNAVIAQARERHAKN
                     LWTDKPGNTRPQLVTVADEAGQARWVASRILELREGGLKLKSQAVLFRTSSHSAALEL
                     ELTRCQIPFVKFGGLKFLEAAHVKDVLAILRFAQNPRGRVAGFRVLQLIAGIGPATAA
                     QLLQAMDTATDPCSALERFLVPTNVVAQWRVFTKLYRTLFDQKDAWPADMDLVKRWYL
                     PNLDRLHEDAAMRRLDIENLEHMAAGYASRERFLSELTLDPPQATSDQSGAPHQDEDY
                     VILSTIHSAKGQEWTSVFVLNVVDGCIPSDLGTGSHEEIEEERRLLYVAMTRAKEYLH
                     LMVPHRFYVTQQTALGDRHLYASRTRFIPSALVREFDQVVWPAVSADAGCAGADGVAR
                     MQVRDRARSAWR"
BASE COUNT          500 a          653 c          672 g          553 t
ORIGIN      
        1 cgggcaatct tgccgagctt ggctgactat atttggagcc aaagaaacac ctgtaataat
       61 gcacagcatt acccgctgtc gctgcccgac agacctgccc gccatgccta cctccactgc
      121 cgcagaagcc gacccgttcc aggcactcaa tgtcgagcag cgccaggcag ttgaacacgg
      181 cctgggcaat gagagtgctg acacaaggcc cttgctgatc attgccggag ctgggtctgg
      241 caaaaccaac accttggcct accgggtcgc caatctcatc gtgcaccagg ctgatcccca
      301 gcgcatgctg ttgctgacct tttcccgccg ggccgcgcaa gaaatggaac accgtgtagg
      361 ggctgttctg agccgagttt tgggccaaag ctcggctctt cagcttcctt cacttccctg
      421 ggcaggaaca ttccacagca tagcagcgcg gctgctgcgt tgctacgcgg gccgcattgg
      481 tctggatgat tccttcacca ttcttgacag gggtgatgca gaggacctca tgggaatggt
      541 acggcacgac ttgggatttt cttctacaca aaggcgcttt ccgcaaaagg gcacgtgctt
      601 gtcgatatat tcgcgcgtcg tcaacagcca agaacccctc ttgcagatca tgcagcaggt
      661 ctttccctgg tgtgtgcagt ggcaaagtga actcaagagc ctgtttggat gctatgttga
      721 tgccaagcag gcacagaacg tcgtggatta tgacgatctg ttgctcttct ggtcagaaat
      781 ggccgccgag ccgcaactgg ctgccgaatt gggcgaacgc tttgactatg tcctggtcga
      841 tgaataccag gacactaacc gcttgcaggc gtctattttg ttggggatca aaccatcggg
      901 caagggtgtc accgtcgtgg gcgacgatgc gcagagcatc tacagctttc gaggagcgac
      961 ggttcgcaac attcttgatt tcccacgcca gttcggacag ccatcacgcc tggtgaccct
     1021 ggagcgcaac taccgctgca cccagagtat tctggatgtg tccaatgctg tcatcgctca
     1081 agcccgtgaa cgccatgcca agaatctgtg gacagacaaa cccggcaaca cccgtccaca
     1141 gctcgtcacg gttgcggatg aagccggaca ggcacgctgg gtggcaagtc ggattttgga
     1201 actccgcgaa ggtggactca aactgaaatc tcaggccgtg ctttttcgca cgtcttcaca
     1261 cagtgcggcg ttggaacttg aactcacgcg atgccagatt cccttcgtga agtttggtgg
     1321 tctgaagttc ctggaggcag cgcacgtgaa ggatgtgctg gccattctgc gttttgccca
     1381 aaatcccaga ggacgagtag caggattcag ggtattgcag ctcattgccg ggattggacc
     1441 cgccacggcg gcgcagttgc tccaagccat ggatacggcg acagatcctt gttcagcact
     1501 ggagcgtttt ctggttccga cgaatgtagt tgcacagtgg agggttttta cgaagttgta
     1561 ccggacattg tttgaccaga aggatgcctg gccggccgat atggatctgg tcaagcgctg
     1621 gtacctgccg aatctggatc ggctgcacga ggatgcggcg atgcgacgac tcgatatcga
     1681 gaatctggag catatggctg caggctacgc tagccgggag cgattcctga gtgagttgac
     1741 acttgacccc ccgcaagcta ccagcgatca atcaggtgcg ccccatcaag acgaagacta
     1801 cgtcatcctg tcgaccatcc attctgcaaa aggacaggaa tggacttcag tatttgtcct
     1861 gaacgtggtt gatggttgta ttccttctga tttgggaacg ggttcccacg aagagattga
     1921 ggaagagcgc cgactgctgt atgttgcgat gacccgagcc aaagagtact tgcacctgat
     1981 ggtgccgcac cgcttttatg tgacacagca gaccgccttg ggagaccgcc atctctatgc
     2041 ttcgcgcacc cgcttcatcc ccagtgcgct agttagagag ttcgatcagg tcgtatggcc
     2101 cgctgtctct gcagatgccg ggtgcgcagg ggctgacggg gttgcccgaa tgcaggtgcg
     2161 tgaccgtgca cgttcagcct ggcgttgaac gctagccttc ttccgcagcc cgacttgcac
     2221 cggatgaaca tcaagatcct agccagtttg gttgatggtc ttgtcgctac gcaaagcctc
     2281 caacccgacc ttggcgttgt actctggtgt gtggaacttg cgtttcttcg tttcactcat
     2341 gattttggat ttccttgatt tgtggacatc ttaaattt
//
LOCUS       DAOSJM010000369         2357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_39085_length_2357_cov_5.929626, whole genome shotgun sequence.
ACCESSION   DAOSJM010000369 DAOSJM010000000
VERSION     DAOSJM010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39085_length_2357_cov_5.929626"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PKX82_11390"
     CDS             <1..817
                     /locus_tag="PKX82_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HOF52903.1"
                     /translation="NTAFYFVDESGDGVLFNAKGQVMVGQPGGAMGHFIMGLLQVEDP
                     TTLAVELEQLRAELAADPYFRDVPSFQPGRGKTAVAFHAKDDLPEVRREVFKLLLRHP
                     VKFYAVVRDMQAVLAYVRERNARGGNYRYHPNELYDNTVARLFKNRLHQYESCHVCYA
                     VRGSSDRTRAFASALELARERFARQWDKQVATAIQVTPARSAQQACLQAVDYLLWALQ
                     RHYTRQESRFLDMMWGKVSLIHAVDETDKAPYGVYYSKTKPLTGAALRNEVGI"
     gene            1165..>2357
                     /locus_tag="PKX82_11395"
     CDS             1165..>2357
                     /locus_tag="PKX82_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOF52904.1"
                     /translation="MPQITLKSYQQAALNALGAFARAAQMKGPALAFGEQAGRPYNPD
                     PFGAVPCVCLRIPTGGGKTVLAAHAVPLLARAWRATDAPVAVWLVPSDTIRSQTLKAL
                     QTAGHPYRAALEAHYGPGVKVCALEDVAQIAPPDWGRQAVVVVATIQSFRIEETGQRN
                     VYSFSEAFEPHFRSTDAQHLAVLQPLPDAVVTAEEAAQDGKGLLKGFVGQARWSLANW
                     LALHEPILIVDEAHNTKTDKSFTALKRLNPSFILELTATPIPAKTNVLYHVSAQELAA
                     ESMIKLPIALAEHPQGWPQAVFAAVQTQRGLEAEALKDEAEGGYGDKGYVRPIVLFQA
                     QNADDEMPPEALRRYLIDELHIPANQIVVATGDVRGLDGLDLSARNCPVRFVITVQAL
                     REGWD"
BASE COUNT          469 a          713 c          785 g          390 t
ORIGIN      
        1 gaacaccgcg ttttactttg tcgatgaatc cggcgatggc gtgctgttca acgccaaggg
       61 gcaggtcatg gtgggtcagc ccggtggcgc gatgggccac ttcatcatgg gtttgctgca
      121 agtggaagat ccgacgacgc tggcggtgga actggagcaa ctgcgcgcag agctcgccgc
      181 ggacccatat ttcagggatg tgccctcgtt ccagccgggg cgcggcaaga cggcggtggc
      241 gtttcacgcc aaggacgatt tgcccgaggt ccgccgggag gtgttcaagc tgttgctgcg
      301 ccacccggtg aagttctacg cggtggtgcg cgacatgcag gcggtgctgg cctacgtgcg
      361 cgaacgcaat gcgcgcggcg gaaactaccg ctaccacccc aacgagttgt acgacaacac
      421 cgtcgcgcgg ctgttcaaga atcgcctgca ccaatatgaa agctgccacg tctgctatgc
      481 cgttcggggc agttcggatc ggaccagggc tttcgcctcg gcgctggaac tggcccgcga
      541 gcggttcgcc cggcaatggg acaaacaggt cgccaccgcc atccaggtca cgccggctcg
      601 gtcggcgcag caggcctgtc tgcaagcggt ggactacctt ctgtgggcct tgcagcggca
      661 ttacacccgt caggagagcc gctttctgga catgatgtgg ggcaaggtca gtctcatcca
      721 cgcggtggac gaaaccgaca aggcgcctta cggggtgtat tactcaaaaa caaagccgct
      781 cacgggagcg gctttgagaa acgaggtggg aatataggat cgcctgactg ggttgccccg
      841 tggttccagc tctcacacgg catggagccg aattttgttc ccacggggcg attgtaaatt
      901 gtgtttaccg atctggcatc cccgcccaac ccgcatcacg tcaaggcgca atgaagaaaa
      961 aatgaggaaa gaatggcaac actgaaattg aagacaccgc caacccaagc tggcgagcga
     1021 tacggacgct ggccgcgcca atgagcgctg aagaagcagg atgaatccgc caatcaggaa
     1081 gaaaaactta atttaaatca acagtttgca ttggcgtagt cgtttcaact caaccaatgc
     1141 ttgcgccaat tagccccgct ccccatgccc cagatcaccc tcaagtccta ccagcaggcc
     1201 gcactgaatg cgctgggggc gtttgcccgc gcggcgcaga tgaaggggcc ggcgctggcg
     1261 tttggcgagc aggcggggcg gccctacaac ccggacccgt ttggcgcggt gccctgcgtg
     1321 tgcctgcgca tccccacggg cgggggcaag acggtgttgg cggcgcacgc ggtgccgctg
     1381 ctggcgcgcg catggcgggc cacggacgcg ccggtggcgg tgtggctggt gcccagcgac
     1441 accatccgca gccagacgct caaggcgctg cagacggcgg gccacccgta ccgcgcagcg
     1501 ctggaagccc attacggccc tggcgtgaag gtctgcgcgc tggaagacgt ggcgcagatc
     1561 gcgccgccgg actgggggcg ccaggcggtg gtcgtggtgg cgacgatcca gagtttccgc
     1621 atcgaggaaa ccgggcagcg caacgtctac agcttctcgg aggctttcga gccgcacttc
     1681 cggagcacgg acgcacaaca cctggcggtg ctgcagcccc tgcccgacgc ggtggtgaca
     1741 gctgaagagg cggcacagga tggtaaaggc cttcttaagg gttttgtcgg ccaagcgcgc
     1801 tggagcctgg cgaactggct ggcgctgcac gagccgatcc tgatcgtgga cgaggcgcac
     1861 aacaccaaga ccgacaagag cttcaccgcg ctgaaaaggc ttaacccatc gttcattttg
     1921 gagttgacgg ccacgccgat tccggcaaaa accaacgtgc tttaccacgt cagcgcgcag
     1981 gagctggcag cggaaagcat gatcaaactg ccgattgccc tggccgagca tccgcagggc
     2041 tggccgcaag ccgtctttgc agcagtgcaa acccagcgtg ggctggaagc cgaggcgctg
     2101 aaggacgagg ccgagggagg ctatggcgac aagggctacg tgcgccccat cgtgctgttc
     2161 caggcgcaaa acgcggatga cgagatgccg cccgaggcac tgaggcgcta cctgatcgac
     2221 gagctgcaca ttccggccaa ccagatcgtg gtggccaccg gcgatgtgcg cggcctggac
     2281 gggctggacc tgagcgcgcg caactgcccg gtgcgcttcg tcatcaccgt gcaggcgctg
     2341 cgcgagggct gggactg
//
LOCUS       DAOSJM010000370         2351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_39205_length_2351_cov_3.282666, whole genome shotgun sequence.
ACCESSION   DAOSJM010000370 DAOSJM010000000
VERSION     DAOSJM010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39205_length_2351_cov_3.282666"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="PKX82_11400"
     CDS             <1..326
                     /locus_tag="PKX82_11400"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOF52905.1"
                     /translation="EGSCAIDEDLLDAANICENEQIHIWNINNGERFVTYAIKGERGS
                     GMISVNGSAARRASNGDLIIIAAFAQVHDDQVAMHEPQLVFVDDKNRQVELRHKVPTQ
                     AAPGH"
     gene            complement(348..1241)
                     /locus_tag="PKX82_11405"
     CDS             complement(348..1241)
                     /locus_tag="PKX82_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOF52906.1"
                     /translation="MDIPIRYISPVFRPPSEAQSLILPVTNGCSWNKCTYCEMYTAPQ
                     KKFAVRDEDETLDTIRQCGAQFGSQVQRVFLGDGDAMALSTRRLMTVLEAIQQHLPAV
                     RRVSTYCLPRNVRSKSVADLQEMRAAGLSLAYVGAESGDDEVLQKVNKGETFASTRDA
                     LDKLGAAGITRSVMLLNGLGGRVLSRQHAENSARLANLTQPEYLATLVVSFPQGEARF
                     RQGFPEWEPLEQGELFGEIEQLLQALELQRTVFRSDHASNWLALKGTLGAEKDRLLKQ
                     VRQAIEAPESAKLRQEWQRGL"
     gene            complement(1244..>2351)
                     /gene="nadA"
                     /locus_tag="PKX82_11410"
     CDS             complement(1244..>2351)
                     /gene="nadA"
                     /locus_tag="PKX82_11410"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOF52907.1"
                     /translation="VDYEQPDTNPAGQPGVCSTRHAWARVPAEPTQTERSALKDKIRH
                     LLKVKNAVMVSHYYVHPDLQDLAEETGGLVSDSLEMARFGRDHAAQTLVVSGVKFMGE
                     TAKILSPHKTVLMPDLDATCSLDLGCPIKEFNAFCDAHPDRTVVVYANTSAAVKARAD
                     WLVTSSCALDIVRALKDKGQKILWAPDKHLGGYIQRETGADMVFWEGACIVHDEFKAF
                     ELEALKKEHPGAKVLVHPESPRDVVALADAVGSTSAILKAAQTMDAKEFIVATDNGMM
                     HKLRTLNPGKTFIEAPTAGNSATCKSCAHCPWMAMNGLAGVARVLEHGLNEIQVDAKL
                     IDRARLPIDRMLAFTAALKNGLPVGGLIPNIGAA"
BASE COUNT          422 a          753 c          725 g          451 t
ORIGIN      
        1 acgaaggctc ctgcgctatc gacgaggacc tgctcgacgc cgccaacatc tgcgagaacg
       61 aacagatcca catctggaat atcaacaatg gcgagcgatt tgtgacctat gccatcaagg
      121 gggaacgtgg ctccggcatg atctcggtga acggctcggc cgcacggcgc gcctccaacg
      181 gcgacctcat catcattgca gcgtttgccc aggtgcacga cgaccaggtg gccatgcatg
      241 aaccccagct ggtgttcgta gacgacaaga atcgccaggt ggaattgcgc cacaaggtac
      301 cgacccaggc agctccgggc cactgagccc atcagccccg acagacgtca gagaccgcgc
      361 tgccactcct ggcgcaattt ggcggattca ggtgcctcga tggcctggcg cacctgtttc
      421 agaaggcggt ctttctcggc tcccagcgtg cccttgagcg ccagccagtt ggacgcatgg
      481 tcgctgcgaa aaacggtgcg ctggagttcc aacgcctgta gcagctgttc gatctcgcca
      541 aacagttcgc cttgttccag tggctcccac tcgggaaagc cctgccgaaa ccgggcctcc
      601 ccctgcggaa aactcaccac cagcgtggcc aggtattcgg gctgcgtgag gttggccaga
      661 cgcgccgagt tctcggcatg ctggcgcgac agcacgcgcc cgcccagccc attgagcagc
      721 atgaccgagc gggtgatccc cgccgctccc agcttgtcca gcgcatcgcg ggtggacgca
      781 aacgtctcgc ccttgttgac tttctgcagg acttcatcgt caccggactc tgcgcccacg
      841 tatgccaggc tcagtccggc ggcgcgcatc tcctgcaggt ccgcgaccga cttgctgcgc
      901 acattgcggg gcaagcaata ggtggaaaca cgccgcactg ccggcaggtg ctgctggatc
      961 gcctccagca cggtcatgag ccggcgtgtg gacagcgcca tggcatcacc atcgcccaga
     1021 aacacgcgct gcacctgcga cccgaactgc gcgccgcact ggcggatcgt atccagggtt
     1081 tcgtcttcgt cgcgcaccgc aaatttcttc tgtggcgcgg tgtacatctc acagtaggtg
     1141 cacttattcc acgagcagcc attggtcacc ggcaggatca gcgactgcgc ctcgctgggc
     1201 ggacgaaaca ccgggctgat gtagcggatc ggaatgtcca tgctcaggcg gcgccgatgt
     1261 tcggaatcag tccacccaca ggcagtccat ttttgagggc cgcggtaaac gccagcatgc
     1321 ggtcaatcgg caggcgcgca cggtcaatga gtttcgcgtc cacctggatt tcgttgagcc
     1381 catgctccag cacccgggcc acaccggcca agccgttcat ggccatccag gggcaatgcg
     1441 cgcagctctt gcaggtggcg ctgttgcccg cggtaggcgc ctcaataaaa gtcttgccgg
     1501 ggttgagcgt gcgcagcttg tgcatcatgc cgttgtcggt agcaacgatg aactccttgg
     1561 cgtccatggt ctgtgcggcc ttgaggatgg cgctggtgga gcccaccgca tcagcgaggg
     1621 ccaccacgtc tctgggcgac tcggggtgca ccagcacctt ggcgcctggg tgttctttct
     1681 tcagcgcttc cagctcgaac gccttgaact cgtcgtgcac gatgcaggcg ccttcccaga
     1741 acaccatgtc ggcgccggtt tcacgctgga tatagccacc caggtgcttg tcgggcgccc
     1801 acagaatctt ctggcccttg tccttgagcg cgcgcacgat gtccagtgcg caactgctgg
     1861 tcaccagcca gtcggcgcgg gctttgaccg cagcgctggt gttggcatag accaccacgg
     1921 tgcggtccgg gtgggcatcg caaaaagcgt tgaactcttt gatcgggcag cccaggtcga
     1981 gtgaacaggt ggcgtccagg tcgggcatca gcacggtctt gtgcggcgac agaatcttgg
     2041 ctgtttcgcc catgaacttc acgccagaca ccaccagcgt ttgcgccgca tggtcacggc
     2101 caaagcgggc catttccagt gaatcgctga ccaggccacc ggtctcctcg gccaggtcct
     2161 gcaggtcggg gtgcacatag tagtgcgaca ccatgacggc gtttttcacc ttcagcaggt
     2221 ggcggatttt gtctttcagc gcggagcgct cagtctgtgt gggttctgcc ggaacgcggg
     2281 cccaagcgtg gcgggtgctg cagacgccag gctgacctgc ggggttggtg tcaggctgtt
     2341 cgtagtcgac a
//
LOCUS       DAOSJM010000371         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_39458_length_2340_cov_3.318162, whole genome shotgun sequence.
ACCESSION   DAOSJM010000371 DAOSJM010000000
VERSION     DAOSJM010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39458_length_2340_cov_3.318162"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1191)
                     /locus_tag="PKX82_11415"
     CDS             complement(73..1191)
                     /locus_tag="PKX82_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HOF52908.1"
                     /translation="MTDSKTPRRRNLLKGAAVAAGAMSAPMIAKAQTGPINMRWQSTW
                     PAKDIFHEYALDYAKKVNDMTGGDLKIEVLPAGSVVPAFGLLEAVSKGTLDGGHGVLG
                     YHYGKQNALALWNSGPAFGMDANMILAWHKYGGGAELLAKLYASIGGNVQSFLYGPMA
                     TQPLGWFKKPITKSADFKGLKFRTNGLAIDLFTAMGAAVNALPGGEIVPAMDRGLLDG
                     AEFNNASSDRLLGFPDVSKVCMLQSYHQSAETFEIMFNKTKYDALPAKMKAIIAGATE
                     AASADMSWKAIDRYSKDYIELQTKDKVKFYKTPDSVLQDQLKLWTEIVEKKSAENPLF
                     KEIVASQKAFAARAVKWDQDTYVSRRMAVNHFFGAKKA"
     gene            1435..>2340
                     /locus_tag="PKX82_11420"
     CDS             1435..>2340
                     /locus_tag="PKX82_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOF52909.1"
                     /translation="MQPIQLFDSASSTYTYVLFDEQSREALIIDPVDEQLERDLAVLR
                     QYGLTLLWTVETHAHADHITSAGLLAEHTGAKTAAPQGCGILTAAVQLVDGDTLTFGS
                     QQIRALHTPGHTAGSMSFAWHNHVFTGDTLLINGCGRTDFQSGSPEAMYHSLTQVLFK
                     LPDETTVWPGHDYHGRTHSSIGAEKSGNTRVAGKTLAEFTAIMEALHLPKPKRIDEAV
                     PANLTSGVRHDAGGVGAQEMRPATGYAGDVSAALAYAWWQAGDAVLVDVRTDAEREWV
                     GFVPGAIGVAWKQWPGMAMNANFDAA"
BASE COUNT          498 a          689 c          652 g          501 t
ORIGIN      
        1 catattcaaa ccgcctaagt aaatcagcca tccaggcaac ccccggatgg ctgaaatgtg
       61 aagcgaaaac cgttacgcct tcttggcacc aaagaagtgg ttcaccgcca tacgacggct
      121 gacgtaggtg tcctgatccc acttgacggc gcgtgctgca aacgcttttt gcgacgccac
      181 aatttccttg aacagggggt tctcagcaga cttcttctcc acgatttcgg tccacagttt
      241 gagttggtcc tgcaagacag agtccggggt cttgtagaac ttgaccttgt ccttggtttg
      301 cagctcaatg tagtccttgg agtagcggtc aatggctttc cacgacatgt ctgcagaagc
      361 cgcctcggta gcgcctgcga tgatggcctt catcttcgca ggcagcgcat cgtatttggt
      421 cttgttgaac atgatctcga aggtctcggc gctctggtgg tagctttgca gcatgcagac
      481 tttggagaca tcggggaagc ccagcagacg gtcggagctc gcgttattga actctgcgcc
      541 atcgagcagg ccacggtcca ttgccgggac gatttcgcca cctggcaagg cattcaccgc
      601 ggcgcccatg gcggtgaaca ggtcgatcgc caacccattg gtacggaact tcaaaccctt
      661 gaaatctgcc gacttggtga tgggtttctt gaaccagccc agaggctgcg tcgccatcgg
      721 accgtacagg aacgactgca cattgccacc aatggaagcg tacagcttgg ccagcagttc
      781 tgcgccaccg ccgtatttgt gccacgccag aatcatgttg gcatccatgc caaatgccgg
      841 gccggaattc cagagtgcca gggcgttctg cttgccatag tggtagccca gcactccgtg
      901 tccaccgtcc agcgtgccct tggaaaccgc ttccagcaaa ccaaatgccg gcaccacaga
      961 accagcaggc agaacctcga tcttgagatc gccaccggtc atgtcattga ccttcttggc
     1021 gtagtccagt gcgtactcgt gaaaaatatc cttggcaggc caggtgcttt gccaacgcat
     1081 gttgataggg ccggtttgcg ccttggcaat catgggcgca gacatggcgc ctgcggctac
     1141 ggcggcaccc ttgagcaggt tgcggcggcg tggggttttg ctgtcagtca tgcgttatct
     1201 cctgtagtaa ttaaagaact aacgattgaa tactatgaga ttcagtgtgc ggaacagcaa
     1261 gggttttcac caacccagcg ccatgcattg catctctttt aagcaatact gttttctcat
     1321 ctttgcagct ttgcctttcg acaccgacga tgagacggca tactccaatc caatatccag
     1381 tagagttcgc ctaagtccat tcgcgcccag aattcagtca accacacccg tcacatgcaa
     1441 ccgatccagc tgtttgattc cgcttccagc acttacacct atgttctgtt cgatgaacaa
     1501 tcgcgtgagg cactcatcat tgacccggtg gatgagcaac tcgagcgaga ccttgccgta
     1561 ctgcgccagt acggcctgac actgctatgg acggtagaaa cccacgccca cgcggaccac
     1621 atcacgagcg cgggcctgct ggcagaacac accggcgcaa aaactgctgc gccccaaggc
     1681 tgcgggattc tgactgcagc ggtgcaactc gtcgatggcg acacgttaac ttttggcagc
     1741 cagcaaatca gggcgctgca cacgccgggg cataccgcag gaagcatgag ctttgcctgg
     1801 cacaaccatg tcttcaccgg ggacaccctg ctgatcaacg gctgcggccg aaccgacttc
     1861 cagtcgggca gtcccgaggc gatgtaccac agcctgacgc aggtgctatt caagttgccc
     1921 gacgaaacca ccgtctggcc tggccacgat taccatggtc gcacgcactc cagcattggg
     1981 gctgagaagt ccggcaatac ccgggtggcc ggcaagacgc ttgcagaatt caccgccatc
     2041 atggaagcgc tgcacctacc caaacccaag cgcattgacg aagctgtgcc cgccaacctg
     2101 acctcggggg ttcgccatga tgcgggtggc gttggcgcgc aggaaatgcg tccagccact
     2161 ggctatgccg gtgatgtgtc ggcagcgctg gcctacgcct ggtggcaggc gggtgacgcg
     2221 gtactggtcg atgtgcgtac cgatgcggag cgcgaatggg tgggctttgt tccgggcgct
     2281 attggtgttg cgtggaagca atggcctggc atggccatga acgccaactt tgatgcagcc
//
LOCUS       DAOSJM010000372         2339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_39480_length_2339_cov_4.507443, whole genome shotgun sequence.
ACCESSION   DAOSJM010000372 DAOSJM010000000
VERSION     DAOSJM010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39480_length_2339_cov_4.507443"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="PKX82_11425"
     CDS             <1..1249
                     /locus_tag="PKX82_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOF52910.1"
                     /translation="LAIALLVLAYWWLDPNPPQRVTLATGPAQSAYEEFGKRYASALA
                     KNGIEVVLLPSEGSAANLQLLREGKADLGFVQGGSQEAGAQSGTSDSTPLQSLGSLFL
                     EPVWLFYRESVSPRTPLTSLTQLKGLRVNIGTPGSGVPSLMGKLFESNRVDITALKIS
                     QLEQTPATVAFLGGELDAMVLVSAPESLMVQMLLQTPGVKLMNFAQSEAYSRRFAFLS
                     PAVLPQGVVDLAGNIPPQEVHLVAPTTTLLTRTTTHPALLQLFSLAGNDIHGNAGWFQ
                     HAREYPSANHNEFPLHPEAERSMQRNAPLLQRYLPFWVANLVERMWLAMGIIIAILLP
                     LSRVVPPLYEFRIRSRIFRWYAQLRDIEQRAAGAGPVDGLLEELGQMDRRAELIVVPL
                     SYADELYALRANIQLVRNRLQGR"
     gene            complement(1273..>2339)
                     /locus_tag="PKX82_11430"
     CDS             complement(1273..>2339)
                     /locus_tag="PKX82_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOF52911.1"
                     /translation="SDHLYIYPNGVRLDDSIDAAREIGMRFVATRGSMSVGQSQGGLP
                     PDRVVESEDFILKDTQRLIEQYHDTAHGSMLNVAVAPCSPFSVSRDLMRESALLARQY
                     GARLHTHLAENDHDLTYSREKFNCTPTQYAQDLGWLGHDVWHAHCVKLDEEGISLFAA
                     TRTGVAHCPCSNMRLASGIAPIRKMLNAGVPVGLGVDGSASNDAAHMVNEARQALLLA
                     RVGRAMQPPEERTLPSGERKTFFGCDLGPAEMTPRDVLRMATRGGAQVLGRSDIGHLA
                     VGMCADLVLFDLNTLAFAGGAVHDPVGSLLLCASPQVDTTVVNGRVVVRQGQLATIDL
                     GPLLEKHNALAVQLAQNSPR"
BASE COUNT          373 a          751 c          785 g          430 t
ORIGIN      
        1 actggccatc gctttgctgg tgctggcgta ctggtggctg gacccgaatc caccacagcg
       61 cgtcacgctg gccaccggcc cggcgcaaag cgcctacgaa gaattcggca agcgctatgc
      121 cagtgcactg gccaagaatg gcatcgaggt cgtgctgctg ccctctgagg ggtcggctgc
      181 caacctgcag ctgctgcgcg agggcaaggc cgacctgggc tttgtgcaag gcggcagcca
      241 ggaggccgga gcacagagcg gcacgtcgga cagcaccccg ctgcagtcgc tgggcagcct
      301 gtttctggag ccggtctggc tgttctaccg cgagtcggta tcaccgcgca cgccgctgac
      361 gtctctcacg caactcaaag gtctgcgtgt caacatcggc acgcccggta gtggtgtgcc
      421 cagtctgatg ggcaagctct tcgagagcaa ccgcgtggac atcacggcac tgaagatttc
      481 ccaattggag caaacgccag ccaccgtggc tttccttggc ggggaactcg acgccatggt
      541 gctggtctcg gcgcctgaat cgctgatggt gcagatgctg ctgcaaacac cgggcgtgaa
      601 gctgatgaac tttgcgcaga gcgaggccta ttcacggcgg tttgcgttcc tgagcccggc
      661 ggtgctgccg caaggcgtgg tggacctggc gggcaacatc ccgccgcagg aggtgcatct
      721 ggtggcaccg accaccaccc tgctcacacg caccaccacc cacccggcgc tgctgcagct
      781 gttttcgctg gcgggcaacg acatccatgg caacgcgggc tggttccagc atgcccgtga
      841 atacccgtcg gcaaaccaca acgagttccc cttgcacccc gaggccgaac gcagcatgca
      901 gcgcaatgcg ccgctgctgc agcgttacct gccgttctgg gtggccaatc tggtcgagcg
      961 catgtggctg gccatgggca tcatcatcgc gattttgctg ccgctgtccc gtgtggtgcc
     1021 gccgctgtat gaattccgca tccgttcgcg catctttcgc tggtatgccc aactgcgcga
     1081 tatcgagcag cgtgcggcgg gtgcgggtcc ggtggatggc ctgctggagg agttgggcca
     1141 gatggaccgg cgtgccgaac tgatcgtggt gccgctgtcc tatgccgacg agctgtacgc
     1201 cctgcgtgcc aacatccagt tggtgcgcaa ccggttgcag gggcgttgag gctattgttt
     1261 ttgtagcgaa tgctatcggg gcgaattctg ggcgagctgc accgccagtg cgttgtgttt
     1321 ctccagcagc ggcccgagat cgatggtggc gagctgaccc tggcgcacca ccacgcggcc
     1381 gttgaccacg gtggtgtcga cctgcgggct ggcgcacaac agcaggctgc ccaccgggtc
     1441 atgcaccgcg ccaccggcaa aggccagcgt gttcaggtca aacagcacca ggtcggcgca
     1501 catgcccacg gccagatgtc cgatgtcact gcgccccagt acctgcgcgc cgccgcgcgt
     1561 ggccatgcgc agcacgtcgc ggggcgtcat ctcggccggc cccaggtcgc agccaaaaaa
     1621 ggttttgcgc tcgccgctgg gcagggtgcg ttcctcaggc ggctgcatgg cgcggcccac
     1681 gcgggccagc agcagagcct ggcgcgcttc attgaccata tgcgcggcgt cgttgctggc
     1741 gctgccgtcc acccccagac cgaccggcac gccggcattg agcatttttc gaatgggcgc
     1801 aatgccgctg gccaggcgca tgttgctgca ggggcagtgc gcgacgccgg tgcgcgtggc
     1861 ggcaaacagg ctgatgcctt cctcgtcgag tttcacgcag tgggcgtgcc agacatcgtg
     1921 ccccagccag cccaggtcct gcgcgtactg cgtgggcgtg cagttgaact tctcgcgcga
     1981 gtaggtcagg tcgtggtcgt tttcggccag atgggtgtgc aggcgggcac cgtactgtct
     2041 cgccagcagc gccgactcgc gcatcaggtc gcggctgacg ctgaacgggg agcagggcgc
     2101 taccgccacg ttgagcatgg agccgtgcgc agtgtcgtgg tactgctcga tcaggcgctg
     2161 cgtgtccttg aggatgaagt cttcgctttc cactacgcgg tcgggtggca ggccgccttg
     2221 cgactggccc acgctcatgc tgccacgcgt ggccacaaac cgcatgccga tctcgcgcgc
     2281 ggcgtcgatg ctgtcgtcca ggcgcacacc gttggggtag atgtagaggt ggtcgctgc
//
LOCUS       DAOSJM010000373         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_39855_length_2322_cov_3.589766, whole genome shotgun sequence.
ACCESSION   DAOSJM010000373 DAOSJM010000000
VERSION     DAOSJM010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39855_length_2322_cov_3.589766"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PKX82_11435"
     CDS             <1..711
                     /locus_tag="PKX82_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52912.1"
                     /translation="NVAALQGSMQFADNSVVNVLLDRRAVPVRSLGNVLFFQDPTLAT
                     PARSIRELLQTTPIDMLRSRVNGVTPFQSQASLGYNRPLNARWQVGGNLNYTNVDAIQ
                     PVPDILPNGQPSTGDLYGVGLQLIGSNLYSARDTHVFSLNYMSGPTYTGQMLAYNNMT
                     GFEVWQLEPSLRYYTQSTSDGTKTDRWTPGVRMSYRAIKRVSLDAEVSYEIADTVGPT
                     RKESATRMFYYLGGRFDF"
     gene            728..1357
                     /gene="ccmA"
                     /locus_tag="PKX82_11440"
     CDS             728..1357
                     /gene="ccmA"
                     /locus_tag="PKX82_11440"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HOF52913.1"
                     /translation="MLQVRDVAATRGTSRLFQALNFEVLPGHLLWLRGPNGCGKTTLL
                     RMLAGLARPEAGEVCWDGVPIRKAATFRDDLVYMGHSNGLKDDLTAMESLEFLCRIHG
                     RVFSRSTVDAALRRLGVFHRRKLPMRTLSQGQRKRVALARLALESRSGIWVLDEPFDA
                     LDDAGIATVNELLGEHLLRGGSVMLTSHIPVHIAHAPVKVLMLNKVVQA"
     gene            1354..2022
                     /gene="ccmB"
                     /locus_tag="PKX82_11445"
     CDS             1354..2022
                     /gene="ccmB"
                     /locus_tag="PKX82_11445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOF52914.1"
                     /translation="MSGNAFLSVVGRDLRLASRRPVDAFLPIVFFLVAGSLFPLGVGP
                     EANVLRQIGPGIVWVCALLSAMLSLGGMYGGDYLDGSLEQMLLSPQPAVLIAIAKGLA
                     HWLMTGVPLILAAPVFGLLFDMHTETIAILAVSLLLGTPILSLVGGVGAALTLGLRNG
                     AVLILLLVLPLCIPVLIFGAGAVAAVDAGLSSAGHLSLLAALLLLTSLGAPVATAAAL
                     RISV"
     gene            2019..>2322
                     /locus_tag="PKX82_11450"
     CDS             2019..>2322
                     /locus_tag="PKX82_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HOF52915.1"
                     /translation="MKTLPRFFTFAAPSRFYRLSGLLVPWLYGVALVFAVAGLYVGFF
                     VAPTDATQGESYRIIFVHVPAAWMSMLLYMVMAGWAAVGWAFNARLASMVARAIAPT"
BASE COUNT          393 a          629 c          706 g          594 t
ORIGIN      
        1 aatgtggcag cacttcaggg gtccatgcag tttgcagaca attcagtggt caatgtgttg
       61 ctggatcgcc gtgccgttcc cgtccgttca ttggggaatg tgctgttctt ccaggatccc
      121 acgctggcaa caccagccag aagcatccgc gagttgctgc aaaccacgcc gattgacatg
      181 ctgcgcagca gggtcaatgg cgtgacgcct ttccagtccc aggcttcgct ggggtacaac
      241 cggcctctca acgcccgttg gcaagttggc ggtaacctga actacaccaa tgtggatgcc
      301 atccagccag tacccgatat cctgcccaat ggccagccca gtacaggtga cctctatggt
      361 gtcggactgc aattgattgg ctccaatctc tactctgcgc gtgataccca tgttttcagc
      421 ttgaactaca tgagtggtcc cacttacact ggacaaatgc tggcgtacaa caacatgact
      481 ggctttgaag tctggcagct ggagccatcg ctgcggtact acacgcagtc cacctccgat
      541 ggcaccaaga ccgaccgctg gacgccgggc gtacgcatgt cgtaccgcgc gatcaagcgc
      601 gtatccctgg atgcagaagt ttcctacgaa attgcagaca cggtaggacc gacccgcaag
      661 gagtctgcaa cccgcatgtt ctattacctg ggtggacgtt ttgatttcta gacaggttgg
      721 gggtcatgtg cttcaggttc gcgacgtcgc ggctacgcgt gggacgtcac ggctttttca
      781 agcgttgaat tttgaggtgc tgcccggcca tctgttgtgg ctgcgcggac ccaatggctg
      841 tggaaagacc actttgctgc gcatgcttgc gggactggca cgaccggaag cgggcgaagt
      901 ttgctgggac ggtgtaccta ttcggaaggc ggcgaccttt cgcgatgact tggtgtatat
      961 gggtcacagt aacgggctca aggacgatct gacagccatg gagtcactgg agtttctctg
     1021 tcgcatacat gggcgtgttt tcagccgatc taccgttgat gcagcgctac gtcggcttgg
     1081 ggtgttccat cgccgcaagt tgccaatgcg cacgttgtca cagggtcagc ggaaaagggt
     1141 ggctttggcc cgccttgcac tggaatccag gtccggcatc tgggtgctcg acgagccgtt
     1201 tgatgcgctc gatgacgcgg ggatcgcaac ggtgaacgag ttgcttggtg agcatcttct
     1261 gcgcggtggg agtgtcatgt tgaccagtca cattccggtt cacatcgcgc acgcacctgt
     1321 aaaggtgctc atgctgaaca aggtggttca ggcgtgagtg gtaatgcatt tctttctgtg
     1381 gtcggacgtg acctgcgcct ggcaagcaga cgtcctgtcg acgcgttttt gccgatcgtc
     1441 ttcttcctcg tggcagggag ccttttccca ttgggggtag ggcctgaagc aaatgtcctg
     1501 cgccagatcg gtccgggcat cgtgtgggtt tgcgccctgc tgtctgccat gttgtctctt
     1561 gggggaatgt acggaggtga ttatctggat ggttccctgg agcagatgct gctttcaccc
     1621 caacccgcag tactcatcgc tatcgcaaaa ggactggcac attggctcat gaccggtgtg
     1681 ccgttgatac tggctgcacc tgtgtttgga ctgctatttg acatgcacac cgagaccatt
     1741 gcgatactgg ccgtgagttt gctgcttgga acgccgatcc tgagcctcgt gggcggtgtc
     1801 ggggcggctt tgacgctggg gcttcgcaat ggtgccgtcc tgattcttct gttggtcctt
     1861 ccgctgtgta ttcctgtgtt gatttttggt gcaggtgccg tagcggccgt ggatgccgga
     1921 ttgagttccg cggggcatct atccttgttg gctgccctcc ttctgttgac ctcattgggt
     1981 gcgcctgtgg caactgccgc ggccctgcgt atttctgtat gaagacactc ccccgatttt
     2041 ttacgtttgc agccccctcg cggttttacc gactgtccgg gctgttggtg ccctggctct
     2101 atggcgtagc cctcgttttt gcggttgccg gtctgtatgt ggggtttttc gttgctccga
     2161 ctgatgccac acagggcgag tcgtatcgca tcatctttgt gcatgtgccg gcggcctgga
     2221 tgtccatgct gctgtatatg gtgatggccg gatgggcggc tgtcggctgg gctttcaacg
     2281 cacgcttggc ctccatggtg gccagagcca ttgcaccgac ag
//
LOCUS       DAOSJM010000374         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_40017_length_2315_cov_3.209735, whole genome shotgun sequence.
ACCESSION   DAOSJM010000374 DAOSJM010000000
VERSION     DAOSJM010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40017_length_2315_cov_3.209735"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKX82_11455"
     CDS             complement(<1..261)
                     /locus_tag="PKX82_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOF52916.1"
                     /translation="MRKFDEMYTQLPYEFFSKGEQIRDHYKIYDAWLARQPGDMMAKR
                     REEAEMIFRRVGITFAVYGEKDEDGAGTERLIPFDLIPRIIPA"
     gene            503..751
                     /locus_tag="PKX82_11460"
                     /pseudo
     CDS             503..751
                     /locus_tag="PKX82_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
     gene            866..2221
                     /locus_tag="PKX82_11465"
     CDS             866..2221
                     /locus_tag="PKX82_11465"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOF52917.1"
                     /translation="MALQPRDPSPPQGGLRPLQAAGVIQPHPIKAAKPPPPQDTRWRL
                     HWLLAAPHRLGFFGGALMLAISALWWCIELYARSSGMPVAWSVSPSTAHALTMTLGFM
                     PLFFVGFLFTAGPKWLGMPEPGTRALLPQVLTYLAGWILALVGFHASVLLATAGLVLV
                     AAAWSSLAWKFTDLWRRSRARDKAHATVVTIACLFGVLALWLIAGGLAFGLDTLVRTA
                     TQAALWMFIATVFATVSHCMIPFFSASALPVLDAWRPMWLLWSMVSILWLEGLFAVLD
                     LWLWPLPGALRWLQVAVEAPAGALMLWLAIRWGLVQSLKIRLLAMLHGGFLWLGIALA
                     LQAVSHTLMALSQDQFSLGLAPLHAMTMGYLGATLFAMATRVASGHSGRPLAADNIAW
                     ALYWVAQLAVLLRVVSALWPAAGHWPLLLAISAWTLASCGWSLRYGSWFGRPRVDGRP
                     G"
BASE COUNT          372 a          770 c          709 g          464 t
ORIGIN      
        1 ggccgggatg atgcgcggaa tcaggtcgaa cggaatcagc cgctcagtgc cggcgccgtc
       61 ctcatccttc tcgccataga ccgcgaaggt gatacccacg cggcggaaga tcatttccgc
      121 ctcttcgcgg cgcttggcca tcatgtcgcc gggctggcgg gccagccagg catcgtaaat
      181 tttgtagtgg tcccgtatct gctcgccctt cgagaagaac tcgtaaggca actgggtgta
      241 catctcgtca aatttccgca taaatagacc tctctcatcc cactgtaagc aagcttcgga
      301 ccaccacact atgcaccact atggcacaag aattttccac gcaccagaaa gaacaccctt
      361 cgcgaccagg ggtgcagagt cagacattga tccgggtcag tgaatccaaa aatttgctcc
      421 aaattaaggc gcagatggcc cttgccccat acgctccaga aggtcccgca tactgcagac
      481 ggggcaccac caaaggaaaa ccgtgaacac tgcctccacc ttgtccctcc ccgcccacgg
      541 ctttgatgcc cgaggcgttg ccaaacggtt ccgccacgct gccatttttg gcgcactcga
      601 gtctctcaac catggcgaaa ccatgcgctt cgtcaacgac catgacccat tgcccctgct
      661 gcagcaaatc tcgcagcgct acggaaacca gatcaccgcg gcctatgtgg cgcgggagcc
      721 gggcaacatc gtgatcgact tcaccgtgca gctgggcgca gaacaggccg gtgaggccca
      781 acaggcaccg gcctcatcca gctccggcgg ttgcggttcg ggcggttgcg gctgctcggg
      841 tagccactaa agtccctgta tccccatggc actgcaaccc cgcgatccct cgcccccgca
      901 aggtggcttg cgccccctgc aggccgcggg tgtgatacag ccccacccta tcaaggcggc
      961 caagccgcct ccaccccaag acacccgctg gcgcctgcac tggctgctgg cagcgccgca
     1021 ccgcctgggc tttttcggtg gtgcgctgat gctggccatc agtgcactat ggtggtgcat
     1081 agagttgtac gcacgcagca gcgggatgcc ggtcgcctgg tcggtgagcc ccagtaccgc
     1141 ccatgcactg accatgacac tggggttcat gcccctgttt tttgtgggct tcctgttcac
     1201 ggccggcccc aagtggctgg gcatgcccga gcctggcacc cgggccctgc tgccccaggt
     1261 gttgacttac ctggccggct ggatactcgc actggtgggg tttcatgcct ctgtcctgct
     1321 ggccaccgcc ggactggtgc tggtcgctgc cgcctggtcc agcctggcct ggaaattcac
     1381 cgatctctgg cgccgtagcc gtgcccggga caaggcccac gccactgtcg tcacgatcgc
     1441 ctgcctgttc ggcgtgctgg cgctgtggtt gattgccggc gggctggcct tcggactgga
     1501 cacactggta cgcaccgcca cgcaagctgc actgtggatg ttcattgcca ccgtgtttgc
     1561 cacggtttcg cactgcatga ttccgttttt cagcgccagc gctctgccgg tgctcgatgc
     1621 ctggcgtccc atgtggctgt tgtggagcat ggtctccatc ctctggctgg aggggctgtt
     1681 tgcggtactg gacctctggc tgtggcccct gcctggcgcc ctgcgctggc tgcaggtggc
     1741 tgtggaagcg ccggcgggag cactgatgct gtggctggcc attcgttggg gcctggtgca
     1801 aagcctgaaa atccgcctgc tggcgatgct gcacggcggc ttcttgtggc tgggcattgc
     1861 gttggcactg caggccgtat cacacaccct gatggcgctg agccaggacc agttctctct
     1921 ggggctcgcg ccgctgcacg ccatgaccat gggttacctg ggtgccaccc tgtttgccat
     1981 ggcgacccgg gtcgccagcg gccacagcgg gcgaccgctg gcggcggaca acatcgcctg
     2041 ggcactgtac tgggtggcac aactggcggt attgctgcgt gtggtctcgg cactgtggcc
     2101 cgccgctggc cattggcccc tgctgctggc catcagcgcc tggaccctgg ccagctgcgg
     2161 ctggtcgctg cgctacggca gctggtttgg ccgcccgcgt gtggatgggc gtccgggcta
     2221 ggctggcagc cctcagtcgt gccggtaact gaacgtatcg aacaaggcgt ccatttcagc
     2281 cagtagcggc gcatccagcc gcactgcgga cgcgg
//
LOCUS       DAOSJM010000375         2312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_40061_length_2312_cov_5.373505, whole genome shotgun sequence.
ACCESSION   DAOSJM010000375 DAOSJM010000000
VERSION     DAOSJM010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40061_length_2312_cov_5.373505"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKX82_11470"
     CDS             complement(<1..234)
                     /locus_tag="PKX82_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOF52918.1"
                     /translation="MTLFTRLKDWARRIKRDAVAVYFASRDPRTPWLARILAVAIAAY
                     ALSPVDLIPDFIPVLGYLDDVLLVPLGLWLVIHF"
     gene            complement(258..770)
                     /locus_tag="PKX82_11475"
     CDS             complement(258..770)
                     /locus_tag="PKX82_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOF52919.1"
                     /translation="MDTYHASLEKHRINVLRGFEILDTTAEQPYDDVVFLASQICQTP
                     IALITLVDEDRQWFKAKVGLDINETPRNQAFCAHAIQQPTKIFEVEDATKDPRFIDNP
                     LVTGAPDIRFYAGVPLVAATGEALGTVCVIDREPRTLTMAQAASLRALGRLVMSMLEM
                     RRELHKDKAN"
     gene            complement(782..1366)
                     /locus_tag="PKX82_11480"
     CDS             complement(782..1366)
                     /locus_tag="PKX82_11480"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOF52920.1"
                     /translation="MERCPWCEGFDLYREYHDCEWGVPLRDDRALFELLILEGAQAGL
                     SWSTVLKKRESYREAFDHFAPEKIALYGDQKVAELLANPGIIRNRLKVAATIANARAY
                     LQLQESGQTFSEFLWAFVGGKPLQNARRSLSEVPAKTAESDAMSKALSKAGFKFVGST
                     ICYAFMQASGMVNDHLVTCHRHAEVRRASMLPVA"
     gene            complement(1463..>2312)
                     /locus_tag="PKX82_11485"
     CDS             complement(1463..>2312)
                     /locus_tag="PKX82_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF52921.1"
                     /translation="LIAGAVISGYGDAATKAAWLPGIASGEKLVVLAYQERAARYKLD
                     VCEAKATQAPGGYVINATKTVVPVGDKADAFIVPAKVNSQLALFIVERNSAGVTTSGY
                     GTQEGGRAADVVLKNAPATLLTMDGQTALEHAVDIGIAATCAEAVGVMDKTLAITAEY
                     LNTRKQFGVVISSFQALRHRAADMKMQLELARSMSYYASLKLNAPAAERRAALARAKY
                     QLGNSMRFIGQQAVQLHGGIGVTDEYIVSHYFKKLTQMEMTFGDTLHHLGEVSARMQD
                     TAGVFA"
BASE COUNT          455 a          677 c          693 g          487 t
ORIGIN      
        1 gaagtggatc accagccaca accccagggg aaccaagagc acatcgtcca gataacccag
       61 cacaggaatg aagtcaggaa tcagatcgac aggactgagc gcatacgccg caatggcaac
      121 cgccagaatc cgggccaacc acggcgtgcg cgggtcacgt gatgcaaagt aaacggcgac
      181 cgcatcgcgc ttgatacgac gggcccagtc tttcaatcgg gtgaacaagg tcatggatca
      241 gccagtctac aagttggtca attcgctttg tctttgtgta actctctgcg catctccagc
      301 atggacatca ccagcctgcc aagtgccctc aatgaagccg cttgtgccat ggtcaatgtc
      361 ctcggttctc tatcgatcac gcagacagtc cccaaagctt ctcccgttgc ggcaaccaac
      421 ggaactccag cgtaaaacct gatgtcaggt gcgccggtca caagtgggtt gtcgatgaat
      481 cttggatctt tggtcgcatc ctccacctcg aagatcttgg taggctgttg aatggcgtgg
      541 gcgcaaaacg cctgattcct gggtgtttcg ttgatatcca gtcccacttt tgccttgaac
      601 cactgtcgat cctcgtccac caaagtgatc aacgcgatag gcgtttggca tatttgcgat
      661 gcaagaaaca ccacgtcgtc atagggttgc tccgccgtgg tgtcgagaat ctcgaagccg
      721 cgaaggacat tgatgcgatg cttttccaac gatgcgtggt aggtgtccat atttttctcc
      781 tctaggcgac aggcaacatg ctagcgcgcc gaacctccgc atgccggtgg caggtcacca
      841 gatggtcgtt gaccatgccg gaggcctgca tgaacgcgta gcaaatggtg gagccgacaa
      901 acttgaaccc cgccttggac agtgccttgc tcatggcatc cgactcggcc gttttggcgg
      961 ggacttcact caaggatctg cgcgcattct gcagcggctt gccacccaca aacgcccaca
     1021 agaattcgct gaatgtttgc ccgctctctt ggagttgcag gtaggccctg gcgttggcga
     1081 tggtcgccgc gactttcagg cggttgcgga tgatgccggg gttggccagc aactcggcaa
     1141 ctttctggtc gccatagagg gcgatcttct ccggggcaaa gtggtcgaag gcttcgcggt
     1201 agctttcccg tttcttgagc acggtggacc acgacaggcc ggcctgcgcg ccttcgagga
     1261 tcagcaactc gaacagcgcc cggtcgtcgc gcagcggcac tccccattca cagtcatggt
     1321 attcgcggta caggtcaaaa ccttcacacc agggacaacg ttccattgag cacctcgctt
     1381 gtttccacgg gagtatggcg tcaaataaaa aagccttcca gtgcagcact ggaaggcttg
     1441 gggtcagtga cggtctgaac gcttacgcaa acacgcccgc ggtgtcctgc atgcgggcag
     1501 acacttcacc caggtggtgc agggtgtcgc caaaggtcat ttccatctgg gtcagcttct
     1561 tgaagtagtg gctgacgatg tattcgtctg tcacaccgat gccgccgtgc agctgcaccg
     1621 cctgctggcc gataaagcgc atgctgttgc ccagctggta cttggcgcgg gccagggcgg
     1681 cgcggcgctc ggcggcaggg gcgttgagct tgaggctggc gtagtagctc atggagcgtg
     1741 ccagttccag ctgcatcttc atgtcggcag cgcggtggcg cagggcctgg aagctgctga
     1801 tcaccacacc aaactgcttg cgggtgttga ggtattccgc ggtaatggcc agggtcttgt
     1861 ccatcacgcc cacggcttca gcgcaggtgg cggcaatgcc gatgtccacc gcgtgctcca
     1921 gggcggtctg gccgtccatg gtcagcaggg tagcgggggc attcttcagc accacatcgg
     1981 cggcgcgtcc gccttcctgg gtgccgtagc cgctggtggt gacaccggcg gaattgcgct
     2041 ccacgatgaa cagcgccagc tggctgttga ccttggcggg cacgatgaag gcatcggcct
     2101 tgtcacccac gggtactaca gtttttgtag cattgatcac atagccgccg ggggcttgcg
     2161 tggcttttgc ttcgcaaacg tcgagcttgt agcgggcagc gcgttcctgg taggccagaa
     2221 ccaccagctt ctcgccgctg gcaatgccag gcagccaagc ggctttggtg gcagcgtcgc
     2281 catagccgga gatgaccgcg cccgcgatca gg
//
LOCUS       DAOSJM010000376         2309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_40131_length_2309_cov_4.213842, whole genome shotgun sequence.
ACCESSION   DAOSJM010000376 DAOSJM010000000
VERSION     DAOSJM010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40131_length_2309_cov_4.213842"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="PKX82_11490"
     CDS             complement(<1..965)
                     /locus_tag="PKX82_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52922.1"
                     /translation="MLLDFFYTLRSAKLPVSVKEYLTLLEALKKGVVGPNTPEPDDDS
                     EPSGYKIDDFYYLARTALVKDEKHYDKFDRAFAAYFKGVEMVADFTKDIPAEWLRKNL
                     EGMLSPEELAKLQAMGWDELMETLKKRLEEQKERHEGGSKWIGTGGTSPFGAYGNNPQ
                     GIRIGQDKSRNKSAVKVWDQRAYKDYDDTQELGTRNIKVALRRLRKFAREGHEDELDL
                     DETISRTAANAGYLDIKMRPERHNNVKVLLLMDVGGTMDEHIARVEELFSATKTEFKH
                     LEFYYFHNCVYDFMWKNNRRRFAEKFETWDIIRKYNKDYKLIFVG"
     gene            complement(974..1570)
                     /locus_tag="PKX82_11495"
     CDS             complement(974..1570)
                     /locus_tag="PKX82_11495"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOF52923.1"
                     /translation="MAFVEPVTLSARGITLVPLALEHETGLRAAAADGELWNIRVTSV
                     PEPENTRKYIEDALSMREAGNRFAFAVTDHASGKVLGSSSYHDILPAVKRVEIGWTWY
                     AKSVQRSHVNTTCKLMLLTHAFETLGCHVVGWRTDNFNFASQAAIERLGAKKDGVIRG
                     HALRRDGTIRDTVMYSLRSGEWPEVKAQLLHQLDKPRT"
     gene            complement(1573..1794)
                     /locus_tag="PKX82_11500"
     CDS             complement(1573..1794)
                     /locus_tag="PKX82_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52924.1"
                     /translation="MKNDLLIEGISDAIGFVGGALLGFWAGRLLGLDVFAPGYGNASI
                     AGIVLVGVAGGIGLRLARRWRTSRKQPKE"
     gene            complement(1805..>2309)
                     /locus_tag="PKX82_11505"
     CDS             complement(1805..>2309)
                     /locus_tag="PKX82_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOF52925.1"
                     /translation="IDEIDKADIEFPNDLLREIDRMEFYCYETRQLIKAKHRPLVFIT
                     SNNEKELPDAFLRRCFFHYIKFPDAATMQSIVDVHFPGLKKELVSAAMKTFFEVRNLP
                     GLKKKPSTSELLDWLKLLLAEDIPLEALQSKDDKMAVPPLVGALLKNEQDVTLFEKLM
                     FMQRNNR"
BASE COUNT          402 a          684 c          694 g          529 t
ORIGIN      
        1 tcacccacga agatcagctt gtagtccttg ttgtacttgc ggatgatgtc ccaggtctcg
       61 aacttctcgg caaagcggcg ccggttgttc ttccacatga agtcgtacac gcagttgtgg
      121 aagtaataaa actccaggtg tttgaactcc gtcttggtgg cgctgaacaa ctcttccacg
      181 cgggcaatgt gctcgtccat ggtgccgccc acgtccatga gcagcaacac tttcacattg
      241 ttgtgccgtt cggggcgcat cttgatgtca aggtagcccg cattggccgc ggtgcggctg
      301 atggtttcgt ccaggtccag ctcgtcctca tggccttcgc gggcgaactt gcgcaggcgg
      361 cgcaacgcca ccttgatgtt gcgggtgccc agctcctggg tgtcgtcgta atccttgtag
      421 gcgcgctggt cccagacctt gacggcgctc ttgttacggc tcttgtcctg gccgatgcga
      481 ataccctgcg ggttgttacc gtaagcgcca aaaggcgatg ttccaccggt gccgatccat
      541 ttggaaccgc cttcgtggcg ttccttttgc tcttccagcc gcttcttgag cgtctccatc
      601 agttcgtccc agcccatggc ttgcagcttg gccagctcct cggggctcaa catgccctcc
      661 aggttcttgc gcagccactc cgctggaatg tctttggtga agtcggccac catctccacg
      721 cccttgaagt aggcggcaaa ggcgcggtca aacttgtcgt aatgtttctc gtccttcacc
      781 agcgccgtac gggcgaggta gtagaagtcg tcaatcttgt agcccgatgg ctcgctgtca
      841 tcgtccggct cgggcgtgtt gggtcccacc acgccttttt tgagggcttc aagcagggtg
      901 aggtattcct tgaccgaaac gggcagcttg gccgagcgca gggtgtagaa aaaatccagc
      961 agcataggtc gcctcaggtg cgtggcttgt cgagctggtg cagcaactgc gctttcactt
     1021 ccggccattc gccagaacgc aggctgtaca tcacggtgtc gcggatggtg ccgtcgcggc
     1081 gcaatgcatg gccacggatg acgccgtcct tcttcgcgcc caggcgctca atggcggcct
     1141 gggacgcaaa gttgaagttg tcggtgcgcc agcccaccac atggcagccc agggtttcaa
     1201 aggcatgcgt gagcaacatc agtttgcagg tggtgttgac atggctgcgc tgcacgcttt
     1261 tggcgtacca ggtccagcca atttcaacgc gtttcaccgc gggcaggatg tcgtggtagc
     1321 tggagctgcc cagcaccttg ccgctagcgt ggtcggtcac cgcaaaggca aaccggttgc
     1381 cggcctcgcg catggacaaa gcgtcttcaa tgtacttgcg tgtgttctcc ggctcgggca
     1441 ccgatgtcac gcggatgttc cacagttcgc catctgccgc ggccgcgcgc agtccggttt
     1501 catgctcgag cgccaggggc acgagggtga tgccgcgtgc gctcagggtg acaggttcaa
     1561 cgaatgccat gttcactcct tgggctgctt gcggctggtg cgccagcgcc gtgccagtcg
     1621 caagccaatg ccgccagcca cgcccaccag cacaatgccg gcaatgctgg cgttgccata
     1681 gcctggcgcg aaaacatcca gccccagcag gcgacctgcc cagaacccca gcagtgcacc
     1741 accgacgaat ccaatggcat ccgagatgcc ttcaatcagg agatcgttct tcatgtgggt
     1801 cggctcagcg gttgttgcgt tgcatgaaca tcagcttctc gaatagcgtg acgtcctgtt
     1861 cgttcttcag cagtgcgccg accagcggcg gcaccgccat cttgtcgtcc ttgctctgca
     1921 gcgcctcaag cggaatgtct tcggccagca gcagcttgag ccagtccagc agttcgctgg
     1981 tggagggttt cttcttcagg cccggcaggt tgcgcacctc aaagaaggtt ttcatggcag
     2041 cgctgaccag ttcttttttc aggcccggga agtgcacgtc cacaatgctc tgcatggtgg
     2101 cggcatcagg gaacttgatg tagtggaaga agcagcgacg caggaaggcg tctggcagct
     2161 ctttttcgtt gttggaggta atgaacacca gagggcggtg cttggccttg atcagctggc
     2221 gggtttcgta gcagtagaac tccatgcggt cgatttcgcg cagcaggtcg ttggggaatt
     2281 cgatgtcggc cttgtcgatc tcgtcgatc
//
LOCUS       DAOSJM010000377         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_40400_length_2298_cov_3.536781, whole genome shotgun sequence.
ACCESSION   DAOSJM010000377 DAOSJM010000000
VERSION     DAOSJM010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40400_length_2298_cov_3.536781"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKX82_11510"
     CDS             complement(<1..378)
                     /locus_tag="PKX82_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOF52926.1"
                     /translation="MAETTLWWLLTGGAVAVELLTGTFYLLMLAIGLAAAAIAAHLGA
                     STVVQIVVAALVGGGAVAGWYFKQSRNGAEPRAEANPNVNLDIGEVIQITAWNPDGTA
                     DVQYRGAHWTAIHRAGVSPSAGPH"
     gene            522..>2298
                     /locus_tag="PKX82_11515"
     CDS             522..>2298
                     /locus_tag="PKX82_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOF52927.1"
                     /translation="MKFRFPIIIIDEDFRSENTSGLGIRALAQAIESEGFEVLGVTSY
                     GDLSQFAQQQSRASAFILSIDDEEFTPGPDLDPAVLNLRHFIEEVRRKNLDVPIYVYG
                     ETKTSRHIPNDILRELHGFIHMFEDTPEFVARHIIREAKGYLEGVQPPFFKALLDYAE
                     DGSYSWHCPGHSGGVAFLKSPVGQMYHQFYGENMLRADVCNAVEELGQLLDHNGAIGA
                     SERNAARIFNADHCFFVTNGTSTSNKMVWHHTVAPGDIVVVDRNCHKSNLHAIIMTGA
                     IPVFLKPTRNHFGIIGPIPQSEFEPATIQAKIKANPLLKGVDAKKVKPRILTLTQSTY
                     DGVLYNTETIKSMLDGYVENIHFDEAWLPHAAFHPFYGTYHAMGKNRARPKEAVVYST
                     QSIHKLLAGISQASHVLVQDSQTVKLDKHLFNEAYLMHTSTSPQYSIIASCDVAAAMM
                     EPPGGTALVEESIAEALDFRRAMRKVDEEYGDDWWFKVWGPDKLVDEGIGRAADWIIK
                     GESKSAKANWHGFGKLANGFNMLDPIKSTIVTPGLDLNGKFAKTGIPASIVTKFLAEH
                     GVVVEKTGLYSFFIMFTIGITKGRWN"
BASE COUNT          494 a          709 c          665 g          430 t
ORIGIN      
        1 gtgcggtccg gcgctggggg acacccctgc gcgatggatg gcagtccagt gggcaccgcg
       61 gtattgcaca tcggccgtgc catccgggtt ccaggcggta atctggataa cttccccgat
      121 gtccagattg acattgggat tggcctcagc gcggggctct gcgccatttc ggctttgctt
      181 gaagtaccag ccagccaccg caccaccgcc gaccagggca gcgaccacga tttgcactac
      241 ggtgctggcg cccagatggg ccgcaatcgc ggccgcggcc agtccgatgg ccagcatgag
      301 caggtaaaag gtcccggtga gcagctcgac ggcgacagcg ccaccagtga gcagccacca
      361 gagggtggtt tcagccatgt gcagatgccc cttgcgggct ccatgtgtat tgacaacacc
      421 acattgtggg ccaaaaggtg tgcaccgcac catggaaaac ccatttagtc cttacacttc
      481 gcgcctgttt ttctgttttt ggcctgtcgc tggagcctgc aatgaaattt cgctttccca
      541 tcatcatcat tgacgaagat ttccgctcgg aaaacacgtc gggattgggt atccgtgcgc
      601 tggcgcaggc cattgagtcc gagggttttg aggtgctggg tgtgaccagc tacggggacc
      661 tgagccagtt tgcacagcaa caaagccggg ccagcgcctt cattctgtcc atcgacgacg
      721 aagagttcac acccggtccg gacctggacc ccgcggtgct gaacctgcgc cactttattg
      781 aggaagtgcg ccgcaagaac ctggatgtcc cgatctatgt gtatggcgag accaagacca
      841 gccgccacat ccccaacgac atcctgcgcg aactgcacgg cttcattcac atgtttgaag
      901 acacgcctga gttcgtggcg cgccacatca ttcgcgaggc caagggttac ctggaaggtg
      961 tgcagccgcc attcttcaag gcgctgctgg actacgccga ggacggctcc tacagctggc
     1021 actgccccgg tcactccggt ggtgtggcat ttctgaagag cccggtaggc cagatgtacc
     1081 accagtttta cggcgagaac atgctgcgcg ccgacgtgtg caacgcggtg gaagagttgg
     1141 gtcaattgct ggaccacaac ggagcgattg gcgccagtga gcgcaatgcc gcgcgtattt
     1201 tcaacgctga ccactgcttc tttgtgacca acggcacctc cacctccaac aagatggtgt
     1261 ggcaccacac ggtggcacct ggtgacatcg tggtcgtgga ccgcaactgc cacaagtcca
     1321 acctgcacgc catcatcatg accggggcca tccctgtgtt cctgaagccc acccgcaacc
     1381 atttcggcat catcggtccg atcccgcaga gcgagttcga acccgccacc atccaggcca
     1441 agatcaaggc caacccgctg ctcaagggcg tcgatgccaa gaaggtgaag ccgcgcatcc
     1501 tgacgctcac gcaaagcacc tacgacggtg ttctctacaa caccgaaacc atcaagagca
     1561 tgctggacgg ctatgtggaa aacatccact tcgacgaagc ttggctgccc cacgccgctt
     1621 tccatccgtt ctacggcacc taccacgcca tgggcaaaaa ccgcgcacgc cccaaagagg
     1681 ccgtggtcta cagcacccag tccatccaca aactgctggc tggtatcagc caggccagcc
     1741 atgtgctggt gcaggactcg caaaccgtca agctggacaa gcacttgttc aacgaggcct
     1801 acctgatgca caccagcacc agcccgcaat acagcatcat tgccagctgc gatgtggcgg
     1861 cggccatgat ggagcctccc ggcggcaccg cactggtgga ggagagcatt gcggaggcac
     1921 tggacttccg ccgcgccatg cgcaaggtgg acgaagagta cggtgacgac tggtggttca
     1981 aggtctgggg accggacaaa ctggtggacg aaggtattgg ccgcgctgcg gactggatca
     2041 tcaagggcga gagcaagagc gccaaggcca actggcatgg tttcggcaag ctggccaatg
     2101 gcttcaacat gctggacccg atcaagtcca ccatcgtgac gccaggtctg gatctgaacg
     2161 gcaagttcgc caagaccggt attccggcct ccatcgtgac caagttcctg gcggaacatg
     2221 gtgtggtggt ggaaaagacc ggtttgtaca gcttcttcat catgttcacc atcggcatca
     2281 ccaagggccg ctggaaca
//
LOCUS       DAOSJM010000378         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_40477_length_2295_cov_4.675893, whole genome shotgun sequence.
ACCESSION   DAOSJM010000378 DAOSJM010000000
VERSION     DAOSJM010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40477_length_2295_cov_4.675893"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PKX82_11520"
     CDS             <1..383
                     /locus_tag="PKX82_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52928.1"
                     /translation="NAEEQARTLGEQMLQLMQHNESRLQIPSLRKYLNEATQLALNLV
                     DIRPYCELPARTGEVTISTRLRTELLTQAQTSDGLDLLEHRLEIVLDGLEAQLQRTQE
                     SWRGTLETWIGLSILALLVLDYIS"
     gene            404..811
                     /gene="speD"
                     /locus_tag="PKX82_11525"
     CDS             404..811
                     /gene="speD"
                     /locus_tag="PKX82_11525"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HOF52929.1"
                     /translation="MLPTDASPKDYFCEVDGQRFAGTHLLVELWESDGMDDLQRIETG
                     LTQAALAAHATVLHGYFHVFTPGGGITGVLLLAESHITIHTWPEHRYAAIDVFMCASC
                     DPHNSLPALRECFNPGRMSVQTIRRGLPDSGPV"
     gene            complement(808..1737)
                     /locus_tag="PKX82_11530"
     CDS             complement(808..1737)
                     /locus_tag="PKX82_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF52930.1"
                     /translation="MDKLKQLESFVSIATRGSLTAAANAEGVAPAIMGRRLDGLEERL
                     GVKLLIRTTRRITLTHEGSAFLEDCQRIIADVANAEASVSAGGVKASGHLRITAPGGF
                     GRRHVAPLVPRFRELHPDVTVSLNLSDRIVDIAGEGYDCAVRVGDMPDSSLVSVRLAD
                     NRRLCVATPAYLARHGTPQTPAELARFHCLTLSSDASQTRGWAFRGVNAEGTEVTHFK
                     PGGPMDCSDGQVLHDWCLAGYGIAWRSTWEVESEITAGQLVAVLEDFAAPPNGIYAVF
                     PQRKHMSLRVRLWIDFLKEHYGSAGFWRSVAVC"
     gene            complement(1880..>2295)
                     /locus_tag="PKX82_11535"
     CDS             complement(1880..>2295)
                     /locus_tag="PKX82_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOF52931.1"
                     /translation="RAFGFEDLLADTRLTTNNDRVLARAWFIPLLRERLAHRSAADLA
                     AVFEANGLPFAPITDPQYLFDDPHLQQTGGLAPMELPDGRQTQVPLLPLTLGGERLGL
                     RLDPPKLGEHTDALLQGLGYTAAQVTALHAQGVVL"
BASE COUNT          430 a          744 c          715 g          406 t
ORIGIN      
        1 caaatgccga agagcaagcc aggacactgg gagaacagat gctgcaattg atgcagcaca
       61 acgaatcccg gctacagatt ccatcactgc gcaagtacct gaacgaagcc acgcaactag
      121 ccctcaacct ggtggatatc cgaccctact gcgagcttcc tgcacgcact ggagaggtca
      181 ccatcagtac ccgcctgcgt accgagttgc tcacgcaagc ccaaaccagt gacgggttgg
      241 atctgcttga acaccggctg gagattgtgc tggacgggct ggaggcacaa ctgcagagaa
      301 cccaggaatc ctggcgcggc acattggaaa cctggattgg attgagcatc ctcgccctgc
      361 tggtgctgga ctatatttcg taaccccctt ggcaacccgc acgatgcttc ccaccgacgc
      421 aagccccaaa gactatttct gcgaagtcga tggccagcgc ttcgcgggta cccacctgct
      481 ggttgagctg tgggagtccg acggtatgga cgatcttcag cgcattgaaa caggcctcac
      541 ccaagcagcc ctggccgcgc atgccactgt gctacacggt tacttccatg tcttcacacc
      601 cggcggtggc attacaggtg tactgctgtt ggcagaatct cacatcacaa ttcacacttg
      661 gccagagcac cggtatgccg ccattgatgt cttcatgtgt gcaagctgcg accctcacaa
      721 tagtctgcca gcgcttcggg aatgcttcaa ccctggacgg atgtcggttc agaccatccg
      781 gcgtggactg ccagacagcg gccccgttta gcagacagcc acggaccgcc aaaacccggc
      841 ggagccatag tgttccttga gaaagtcgat ccacaggcgc acgcgcaggg acatgtgttt
      901 gcgctggggg aatacggcgt agatgccgtt gggtggcgcc gcaaagtctt ccagcaccgc
      961 caccagttgt ccggcggtga tctcggactc gacctcccag gtgctgcgcc aggcgatgcc
     1021 gtagccggcc aggcaccagt cgtgcagcac ctgcccgtcc gagcaatcca taggcccccc
     1081 gggcttgaaa tgcgtgacct cagtcccttc ggcgttcacc ccccggaatg cccagccccg
     1141 cgtctgcgag gcgtcgctgg acagcgtgag gcaatggaag cgggcgagtt ctgctggtgt
     1201 ctgcggcgtg ccatggcgtg ccaggtaggc cggtgtggcc acgcacaggc ggcggttgtc
     1261 ggccaggcgc acgctcacca gcgacgagtc aggcatgtcg cccacgcgga ccgcgcagtc
     1321 gtagccttcg ccagcaatgt ccacgatgcg gtcggacagg ttgagcgaca ctgtcacgtc
     1381 agggtgcagt tcgcggaacc tgggcaccag cggcgccacg tggcggcggc caaagccacc
     1441 cggcgcggta atgcgcaggt ggccgctggc cttgacgcca ccggcactca cgctggcctc
     1501 ggcattggcg acatcggcaa tgatgcgctg gcagtcttcg aggaacgcgc tgccttcgtg
     1561 cgtgagcgtg atgcgccggg tggtgcggat cagcagcttc acgcccaggc gttcctccag
     1621 cccatcaagg cggcgcccca tgatggccgg ggccacgccc tcggcgttgg cggcggcggt
     1681 caggctgccg cgcgtggcaa tggatacaaa ggattccagt tgtttgagct tgtccatggt
     1741 tagccccttg acggctgaat ggcagagcgc tctgctccgt gtcccccgcc cgctgcgcgg
     1801 gctcctcctt tacctacgca gagcgcccta ccacccagcc ttctagctcg gatggaatag
     1861 gcgcaagcag ctataggttt catagcacca caccttgcgc atgcagcgca gtcacctgcg
     1921 ctgcggtgta gcccaagccc tgcagcaacg catctgtgtg ctcgccgagc ttgggtgggt
     1981 ccagccgcag ccccagccgc tcgccaccaa gcgtgagggg cagcagcggc acctgcgtct
     2041 gtctgccatc gggcaactcc atgggggcca ggccaccggt ctgctgcagg tgcgggtcgt
     2101 caaacaggta ctggggatcg gtgatcgggg caaacggcag tccattggcc tcgaacactg
     2161 cggccaggtc ggccgcactg cgatgggcca gccgctcgcg cagcaggggg atgaaccagg
     2221 cacgtgccag cacacggtcg ttgttggtgg tcagccgggt gtctgccagc aggtcctcga
     2281 acccgaacgc tcggc
//
LOCUS       DAOSJM010000379         2287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_40654_length_2287_cov_4.419803, whole genome shotgun sequence.
ACCESSION   DAOSJM010000379 DAOSJM010000000
VERSION     DAOSJM010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40654_length_2287_cov_4.419803"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1792)
                     /gene="glgP"
                     /locus_tag="PKX82_11540"
     CDS             complement(<1..1792)
                     /gene="glgP"
                     /locus_tag="PKX82_11540"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005977 - glycogen metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HOF52932.1"
                     /translation="MPTVHPFSFDHPTNSVEALRRSISNRLVYAVGKDVRSATRRDWL
                     FAILHAVRDRVMDSWRESLNTAQDKDVKRVYYLSMEFLMGRMLTNALMAVGIYDDTKA
                     ACAQLGADFEGLLDLEADPGLGNGGLGRLAACFLDSIATLGLPGMGYGIRYDYGMFAQ
                     RIVDGRQVEEPDGWLVNGNPWEFMRPEFSYDVRFGGRLTTEGGKVHWVDTDDVVATAY
                     DSAVPGFELTSVATLRLWSARASSVINLDAFNRGDYIGAVEAKNESENVSRVLYPDDS
                     TEHGKELRLRQEYFFVSASLQDLLRRYLKDHGNLDQLCDKVAIHLNDTHPALAVPELM
                     RLLVDEHRMEWDAAWALCCRIFSYTNHTLMQEALETWPVEIMGRLLPRHLRIIYDLNA
                     RFLAGVHERFPEEPDLLRRVSLIEENGHRRVRMASLCVLASHKVNGVSALHSGLMVQT
                     IFADYARLFPDRFCNKTNGITPRRWLAQANQPLAKLIDDRIGAGWRRNLDALTGLRAL
                     ATEPAFNNAVRLAKQQNKRRLADYIARELDIRVDPESLFDVQVKRMHEYKRQLLNLLH
                     VVTRYHQILAHPHANWVPRTVIFAGKAASAY"
     gene            complement(1828..>2287)
                     /locus_tag="PKX82_11545"
     CDS             complement(1828..>2287)
                     /locus_tag="PKX82_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="starch synthase"
                     /protein_id="HOF52933.1"
                     /translation="IEHAIRQAVTRYPGQAVLRVGYDEPLAHRIMAAADVVLVPSRFE
                     PCGLTQLYGLRYGALPLVHAVGGLADTVTDCTAATLADHTASGFTFHEFTPEGLRAAL
                     HRAFDLYRHPADWIAVQHHAMQLRFDWQVAAQAYATLFQRLAPPSGTGPA"
BASE COUNT          420 a          700 c          750 g          417 t
ORIGIN      
        1 ggtaggccga ggcggccttg cccgcaaaga tgacggtgcg cggcacccag ttggcgtgcg
       61 gatgggccag gatctggtgg tagcgtgtga ccacatgcag caggttgagt agctgtcgct
      121 tgtattcgtg catgcgtttg acctgtacgt caaagaggct ttccggatca acccggatgt
      181 ccagctcgcg tgcgatgtag tcggccagcc tgcgcttgtt ctgctgtttg gccagccgca
      241 ccgcgttgtt gaaggccggc tcggtggcca gcgcacgcaa gcccgtcagg gcatccagat
      301 tgcggcgcca tccggccccg atgcggtcgt caatcagttt ggccaggggc tggttggcct
      361 gcgccagcca gcgtctcggg gtgatgccat tggtcttgtt gcaaaagcgg tcggggaaca
      421 gccgtgcgta atctgcaaag atggtctgca ccatcaggcc actgtgcagt gctgacacgc
      481 cgttgacctt gtggctggcc agcacgcaca gggacgccat gcgtacgcgg cggtggccgt
      541 tttcctcgat cagcgagacg cgccgcaaca ggtccggttc ttcgggaaac cgctcgtgca
      601 cccccgccag aaaccgtgca ttgagatcgt agatgatgcg cagatgccga ggtaacaggc
      661 gccccatgat ttctacgggc caggtttcca gcgcctcctg catcagggtg tggttggtgt
      721 agctgaaaat gcggcagcac agggcccacg ccgcatccca ttccatgcga tgttcgtcca
      781 ccagcagacg catgagttct ggcactgcca gcgcggggtg ggtgtcgttc aggtggatgg
      841 ccaccttgtc acagagctgg tccaggttgc catggtcctt gaggtagcgg cgcagcaagt
      901 cctgcagcga cgcactgacg aagaaatact cctggcgcag ccgcagttcc ttgccgtgct
      961 cggtgctgtc gtcggggtac agcacgcgcg agacattctc ggactcgttc ttcgcttcca
     1021 cggcgccgat gtagtcgccc cggttgaacg cgtcgaggtt gatcacactg ctggcgcgtg
     1081 cagaccagag ccgcaacgtg gccaccgacg tcaactcgaa gccgggcacg gcactgtcgt
     1141 aggctgtggc caccacatca tcggtgtcga cccaatggac tttgccgccc tcggtcgtga
     1201 ggcggccacc gaagcgcacg tcgtagctga attccgggcg catgaattcc cagggattgc
     1261 cattgaccag ccagccgtcg ggctcctcga cctggcggcc atccacaatg cgctgggcaa
     1321 acatgccgta gtcgtaccgg atgccatagc ccatgcccgg cagccccagg gtggcgatgg
     1381 agtcgagaaa gcacgccgcc aggcggccca ggccgccatt gcccaggccc gggtcagctt
     1441 ccagatccag taaaccctca aagtcggcac ccagctgggc gcaggcggcc ttggtgtcgt
     1501 cgtagatgcc caccgccatg agtgcattgg tcagcatgcg acccatcagg aattccatgg
     1561 agaggtagta gacgcgtttg acatccttgt cctgcgcggt attgagcgac tcgcgccagc
     1621 tgtccatgac gcgatcacgc acggcatgca ggatggcaaa cagccagtcc cggcgcgtgg
     1681 cggaccgcac gtccttgcca accgcgtaca ccagccggtt cgaaatggac cggcgtagcg
     1741 cctccacgga gttggtgggg tggtcaaaac tgaaggggtg aacggtcggc atgggaaatc
     1801 tccggaaaac gggtaacagc agcagatcta cgcaggaccc gtgccagacg ggggtgccag
     1861 tcgctggaac agcgtcgcat aggcctgcgc tgccacctgc cagtcgaacc gcaactgcat
     1921 ggcgtggtgt tgtaccgcaa tccagtctgc agggtgacgg taaaggtcaa atgcacggtg
     1981 gagtgcagcg cgcaagccct cgggggtgaa ttcgtggaac gtgaacccgc tggcggtgtg
     2041 gtccgccagg gttgcggcgg tgcaatcggt gacggtgtct gccagccccc ccactgcgtg
     2101 caccagaggc agtgcgccat agcgcaatcc gtacaactgc gtcaggccgc agggttcgaa
     2161 ccgtgatggc accagcacca catcggccgc cgccatgatg cggtgtgcca agggctcgtc
     2221 gtagcccacc cgtagcacgg cctgtccggg gtaacgcgtg acggcctgcc gaatggcatg
     2281 ttcgatg
//
LOCUS       DAOSJM010000380         2281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_40792_length_2281_cov_3.861186, whole genome shotgun sequence.
ACCESSION   DAOSJM010000380 DAOSJM010000000
VERSION     DAOSJM010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40792_length_2281_cov_3.861186"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PKX82_11550"
     CDS             complement(<1..607)
                     /locus_tag="PKX82_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding protein"
                     /protein_id="HOF52934.1"
                     /translation="MQRQDSRRFTLKALTAAVALTTLSAVPAFAADTIKVGVLHSLSG
                     TMAISETVLKDTVLMAIDEINAKGGVLGKKLEPVVVDPASNWPLFAEKTKQLLTQDKV
                     AVIFGCWTSVSRKSVLPVVEELNGLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVDY
                     LMSKEGGGAKRWVLLGTDYVYPRTTNKILRAYLKSKGVKDSD"
     gene            complement(766..1650)
                     /locus_tag="PKX82_11555"
     CDS             complement(766..1650)
                     /locus_tag="PKX82_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HOF52935.1"
                     /translation="MTQPAFSTLLYSVDDGIATITLNRPDKLNAFTAQMRDDLIAAFD
                     ATDADDAVRAVIVTGAGRAFCAGADLSTGGQTFDYAARAEEVRDKYKVGDVYRDGGGT
                     VTLRIFQSLKPVIGAINGAAVGIGMTMQLPMDIRMASADARFGFVFSRRGITPEAASS
                     WFLARQVSMQTALEWCYTGRVFGAQEALDHGLIRSVHAPDELLPAARLLAREIADNTA
                     PVSIALTRQLLWRMATAEHPMVAHSVDSRAIQSRGQSADAREGVSAFLEKRAPVYPNK
                     VSTDMPDFYPWWDEPAFR"
     gene            complement(1686..>2281)
                     /locus_tag="PKX82_11560"
     CDS             complement(1686..>2281)
                     /locus_tag="PKX82_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOF52936.1"
                     /translation="LGTGSVTPVQMAGAYSVFANGGFQISPYLIQKVTDTRGTVLFEA
                     KPVAVAQERDRVLDARNAFVMGSMLHEVTVSGTAAAATRTLGRQDLAGKTGTSSEAVD
                     GWFAGFSGATTAVAWMGYDEPKSLGGREFGATLALPIWIDFMRVALNGKPQLPLAPPA
                     GVSYVAGDWVIDEYKDASGAGSIDVDLLQRLKEMFSR"
BASE COUNT          428 a          725 c          719 g          409 t
ORIGIN      
        1 tgtcgctgtc cttcacgccc ttgctcttga ggtaggcgcg caggatcttg ttggtggtgc
       61 gggggtagac atagtcggtg cccagcagca cccagcgctt ggcgccgccg ccttccttgc
      121 tcatcagata gtcaactgcg ggaatggctt gctggttggg tgccgcgccg gtgtagaaca
      181 cgttcttgga cagctcttca ccctcgtact gcacggggta gaacagcagg ccgttgagtt
      241 cttccaccac gggcagcacg gacttgcggg acacagaagt ccagcagcca aagatcacgg
      301 cgaccttgtc ctgcgtcagc agctgttttg tcttctccgc aaacagcggc cagttggagg
      361 cggggtccac gaccacgggt tcgagcttct tgccgagcac accgcccttg gcgttgatct
      421 cgtcaatggc catcaggacc gtgtctttca acacggtctc cgagatggcc atggtgccgg
      481 acaggctgtg cagcacaccg accttgatgg tgtccgcagc aaatgcgggg actgccgaca
      541 aggtggtcag ggcgacggca gcggtgagtg ccttcagggt aaagcgacgg gaatcttggc
      601 gttgcatgtg ggcttctccg gttggtaaac agttcgtggg tcgtggtgcg ccagctgcag
      661 aaagcagctt ttgcgacacg atgggagaac tgtagggaag cagcccagtt cgggaaatac
      721 gccgagtggc gtacatggcg gccacgggca ggccgcagct gtggtttacc ggaaggcggg
      781 ctcgtcccac caggggtaga agtccggcat atccgtggag accttgttcg ggtagaccgg
      841 cgcgcgcttt tccagaaatg cactgacccc ttcgcgcgca tcggccgact ggccgcgcga
      901 ctggatcgcg cggctgtcca cgctgtgggc aaccatcgga tgctcggctg tggccatgcg
      961 ccagagtagc tggcgcgtca gcgctatcga caccggcgcc gtgttgtcgg cgatctcgcg
     1021 cgcgagcaag cgggccgctg gcaacagctc gtcgggtgcg tgcacgctgc ggatcaggcc
     1081 atggtccagc gcctcctgcg cgccaaacac ccggcccgtg taacaccact ccagcgccgt
     1141 ctgcatgctg acctggcgtg ccaggaacca ggacgacgcc gcttcgggcg tgatgccacg
     1201 gcgggaaaac acaaagccga agcgcgcgtc ggcactggcc atacggatgt ccatgggcaa
     1261 ctgcatggtc atgccaatgc ccaccgctgc cccattgatg gcgccgatca ccggcttgag
     1321 gctctgaaag atgcgcaagg tcacggtgcc gccgccatcg cggtacacgt cccccacctt
     1381 gtacttgtcc cgcacctcct ccgcccgcgc tgcgtagtcg aaggtctgac caccggtgga
     1441 aagatcggca ccggcacaga aggctcggcc agccccggta acgatgacgg cgcgcacggc
     1501 gtcgtctgca tcggtggcat cgaacgcggc gatcaggtcg tcacgcatct gcgccgtgaa
     1561 cgcgttcaat ttgtcaggac ggttcagcgt gatggtggcg atgccatcgt caacgctgta
     1621 aagcagggtg gagaatgcag gttgggtcat ggggccatct tggcacagcc cgcaccaacc
     1681 gcctgtcacc gagagaacat ctccttgagg cgctgcaaca ggtccacatc gatggaaccc
     1741 gcaccactgg cgtccttgta ctcgtcaatc acccagtcac ctgccacgta cgacacacct
     1801 gcaggcggcg ccaaaggcag ttgcggtttg ccgttaagcg ccacacgcat gaaatcgatc
     1861 cagatcggca gcgccagggt ggcaccgaac tcacgccctc ccagggactt cggttcgtca
     1921 tagcccatcc aggctacggc ggtggtagca ccactgaagc cggcaaacca gccatccacc
     1981 gcttcactgc tggtgccggt tttgccagcg aggtcctggc gacccagagt tcgggtagcc
     2041 gcagcagcgg tgccggacac ggtcacctca tgcagcatgc tgcccatgac aaaggcattg
     2101 cgcgcatcga gcacccgatc gcgctcctgt gcaacagcca ccggcttggc ctcgaacagc
     2161 acagtgccac gtgtatcggt caccttctgg atcaggtagg ggctgatctg gaaaccccca
     2221 ttggcaaaca ccgagtaggc accggccatc tgcaccggcg tgaccgaacc ggtgcccagc
     2281 g
//
LOCUS       DAOSJM010000381         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_40825_length_2280_cov_3.056629, whole genome shotgun sequence.
ACCESSION   DAOSJM010000381 DAOSJM010000000
VERSION     DAOSJM010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40825_length_2280_cov_3.056629"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PKX82_11565"
     CDS             <1..528
                     /locus_tag="PKX82_11565"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOF52937.1"
                     /translation="VHDATPWEWLTLPLTFVYANFAEYFGHKGPMHHLRPGLRLVYER
                     HTRQHHRFFTETEMAFESPRDFKAVLFPPVLVTFFLLAFGTPMALLLTWLTTPNVGYL
                     FALTAAAYFLNYELLHFAHHLPSEHWVSRLPGMHVLRRLHTDHHDPKLMARYNFNISY
                     PLGDWVFGTLYRGKA"
     gene            complement(610..1809)
                     /locus_tag="PKX82_11570"
     CDS             complement(610..1809)
                     /locus_tag="PKX82_11570"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOF52938.1"
                     /translation="MSKSLTTGTPASLGFCPDRTVGLMHVLQSEVASQRLPGAVALIA
                     RHGQVLLHSSVGMQMPALGKPMALDSIFRIYSMTKPVVSVAILMLMERGKLLLSDPVS
                     KHLPEFANVQVMAHVNSHTVLHHPKAAPTVQDLLRHTSGLTYEILGTEPIQKQYAQAR
                     LASRERSNAEFSQALAAMPLMFEPGTTWEYSRATDLLGALLEVVAGQSLGDFLQHNIF
                     GPLGMVDTAFHVPPEKHHRIAEPFESDPDGGIAMKLIDVRQPARMEAGGAGLVSTSAD
                     YARFLQCLLNGGELDGVRILGLNTVRYMTADHLGTIGVHRAGRSGELLPPGHGFGLGF
                     AVRLEDGIAAAPGTKGLYFWGGIAGTTFFVDPAKDFFALMMIQAPNQRDYYRPLFRNL
                     VYAALLE"
     gene            complement(1821..>2280)
                     /locus_tag="PKX82_11575"
     CDS             complement(1821..>2280)
                     /locus_tag="PKX82_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOF52939.1"
                     /translation="DWGVQALEIAARNLTCTTAVHICYGYGIKANTDWKETLGGEWRQ
                     YAQVFPALAASSIQQVSLECYHSHVPPEMMDLLKGKDVMVGVIDVASDVIETPEQIAD
                     TIGMALQHVPRNRLFPCTNCGLAPMSREVAVKKLEALAQGAALARQRYGA"
BASE COUNT          398 a          736 c          729 g          417 t
ORIGIN      
        1 gtgcacgacg ccacgccatg ggaatggctg acactgccac tgacctttgt ctacgccaat
       61 tttgccgagt actttggcca caagggcccc atgcaccatc tgcgccccgg cctgcgcctg
      121 gtgtacgagc gccacaccag gcagcaccac cgtttcttta ccgaaaccga gatggcgttt
      181 gagagcccgc gcgacttcaa ggcggtgctg ttcccgccgg tgctggtgac cttctttctg
      241 ctggccttcg gcacccccat ggccctgtta ctgacctggc tgacaacacc caatgtgggt
      301 tacctgttcg cactcaccgc cgcggcgtac ttcctgaact acgaactgct gcactttgca
      361 caccacctgc ccagtgagca ctgggtctcg cgtcttccgg gcatgcacgt gttgcgccgc
      421 ctgcacaccg accaccacga cccgaagctg atggcccggt acaacttcaa catcagctac
      481 ccgctgggag actgggtgtt cggtacgctg taccgtggca aagcatgagc acgcatgggg
      541 aatgctccta tttccatagc tacttccgca tattccacgg gggctagagg ccgatttgac
      601 ctataaaccc tattccagca gcgccgcgta caccaggttg cggaacagcg ggcggtagta
      661 gtcgcgctgg ttgggggcct ggatcatcat caaggcgaag aagtccttgg ccgggtccac
      721 aaagaaggtg gtgccggcaa tgccgcccca gaagtacagg cccttggtgc cgggtgcggc
      781 ggcaatgccg tcttccagcc gcaccgcaaa gcccaggcca aagccgtggc ctgggggcag
      841 cagttcaccc gagcggccgg cgcggtgcac gccgatggtg cccagatggt cggccgtcat
      901 gtagcgcacg gtgttgaggc ccagaatgcg cacgccgtca agctcgcccc cgttcagcag
      961 acactgcaaa aagcgcgcgt agtcggcgga ggtggacacc aggcccgcac caccggcttc
     1021 catgcgggcg ggctggcgca cgtcgatcag cttcatggcg atgccgccat cggggtcgct
     1081 ctcgaacggt tcggcaatgc ggtggtgttt ttcgggcggc acatggaagg cggtgtccac
     1141 catgcccagc gggccaaaaa tgttgtgctg caggaagtcg cccagcgact gccctgccac
     1201 cacctccagc agcgcgccca gcaggtcggt ggcgcggctg tattcccacg tagtaccggg
     1261 ctcgaacatc aggggcatgg ccgccagcgc ctgggagaac tcggcgttgc tgcgctcacg
     1321 cgaagccagc cgcgcctgcg cgtactgctt ctggataggc tcggtgccca gaatttcgta
     1381 agtcaggccc gaggtgtggc gcaacaggtc ctgcacggtg ggcgcggcct tggggtggtg
     1441 cagcacggtg tggctgttga catgggccat gacctgcacg ttggcaaact ccggcaggtg
     1501 tttggacacc gggtcgctca gcagcagttt gccgcgctcc atcagcatca ggatggccac
     1561 ggacaccacc ggcttcgtca tggagtagat gcggaagatg gagtccagcg ccatgggctt
     1621 gcccagcgca ggcatttgca tgccgacgct ggaatgcagc agcacctggc cgtggcgcgc
     1681 aatcagcgcg acggcgccgg gcaggcgctg gctggccact tcggactgca ggacatgcat
     1741 caggccgacg gtgcggtcgg ggcaaaagcc caggctggca ggtgtaccgg tggtcaaact
     1801 tttggacata caggcttcct tcaggcgccg tagcgctggc gcgccagggc ggcgccctgg
     1861 gccagggctt cgagtttctt gacggccact tcgcggctca tgggggccag gccgcagtta
     1921 gtgcagggga acaggcgatt gcgtggcaca tgctgcagtg ccatgccaat ggtgtcggca
     1981 atctgctcgg gtgtttcgat cacatcgctg gccacgtcga tcacgcccac catcacgtcc
     2041 ttgcccttca gcaggtccat catctcgggc ggcacatgcg agtggtagca ctccaggctc
     2101 acctgctgga tgctgctggc cgccagcgcc gggaacacct gcgcatactg gcgccactcg
     2161 ccacccaggg tttccttcca gtcggtgttg gccttgatgc cgtacccgta gcagatgtgc
     2221 acggccgtgg tacaggtcag gttgcgggcc gcaatctcca gcgcctgcac gccccagtcg
//
LOCUS       DAOSJM010000382         2268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_41106_length_2268_cov_3.457750, whole genome shotgun sequence.
ACCESSION   DAOSJM010000382 DAOSJM010000000
VERSION     DAOSJM010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41106_length_2268_cov_3.457750"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKX82_11580"
     CDS             <1..505
                     /locus_tag="PKX82_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOF52940.1"
                     /translation="VQGAEIAFSPGFRWGTTLLPGQAITREWLMDMTATTYSYATVTE
                     MTGETIKTVLEDVCDNLFNPDPYYQQGGDMVRVGGLQYSCNPSAGMGKRIDDMRINGK
                     AIEASKKYKVAGWAPVAEEAKGAGNKMVWDVVETWLQSKGGKIAPRKLNTPTLIGVKP
                     NPGYAEA"
     gene            602..1942
                     /locus_tag="PKX82_11585"
     CDS             602..1942
                     /locus_tag="PKX82_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HOF52941.1"
                     /translation="MPSSSIHLPSCLPHDDPLRSQLHNEVHARPQARIRLPALVVYVA
                     VFNDGVTREQECDHLRLLPGQEALGVDQLQGNFLRLRLEGYTLKWERHTEFTRYSLVQ
                     PLPEHAALGAKEPELLSSIVLPPDWLRSIPGRTIAAIQLAMVHGDPGDTQNTLALARD
                     WIGEYPLVASVMGNNSHSLAVTDFILRRDGFERILVIAPPETSRTRAGRVSQRLLELE
                     TYRLMALRGLPVAKGLGPMLSQAESALADITAQLENKSTSDQELLDTLVHLAARVERA
                     TAEHIYRFSATRAYDTLVAQRIVELREKAVPGTQTIGEFMQRRLSPAMATVAATSQRL
                     TSLSERVARTSALLRTRVDIATEVQNQQLLEKLTRGQEMQLRLQTTVEGLSIAAISYY
                     VVSLLVYGGKALKGAGVPVNPEVAAGALIPLVLWGVWRATQRIHRKIHHGASGR"
     gene            complement(1955..>2268)
                     /locus_tag="PKX82_11590"
     CDS             complement(1955..>2268)
                     /locus_tag="PKX82_11590"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOF52942.1"
                     /translation="TSWPTQVREPGGDVEVALVDISVAGAMIKASSNLAAVGAPLKVV
                     MDVTVDGGPMQLILDAKVCHVNKGTHEDAHFIGMAFQNLTREDKLALFYLTQTPQAIQ
                     A"
BASE COUNT          421 a          708 c          732 g          407 t
ORIGIN      
        1 cgtgcagggc gccgagattg cgttctcgcc cggcttccgc tggggtacca cgctgctgcc
       61 gggccaggcc atcacccgcg agtggttgat ggacatgacc gcgaccacct attcctacgc
      121 cacggtgacc gagatgaccg gcgaaactat caagacggtg ctggaagacg tgtgcgacaa
      181 cctgttcaac cccgacccct actaccagca gggtggtgac atggtgcgcg tgggtggcct
      241 gcagtacagc tgcaatccgt ccgccgggat gggcaagcgc attgacgaca tgcgtatcaa
      301 cggcaaggcc atcgaggcca gcaaaaagta caaggtggct ggctgggctc cggtagccga
      361 agaagccaaa ggcgctggca acaagatggt ttgggacgtg gtggaaacct ggctccagtc
      421 caagggcggc aagattgcgc cacgcaagct caatacgccc acgctcattg gtgtgaaacc
      481 caatccggga tacgccgaag cctgagtgcc gcctgcaggc acggattgca gggttatgca
      541 caaaatcggg ggctagcccc cgtaaaatgg gcgcaaccag ctatatattt ggtagcaaac
      601 catgccatcc tcttcgatcc acttgccctc ctgtctgcca catgacgatc ccctgcggtc
      661 ccagttgcac aacgaggtgc atgcccggcc gcaggcacgc atccgcctgc cggccctggt
      721 ggtctatgtg gcggtgttca atgatggtgt gacccgcgag caggaatgcg accacctgcg
      781 gttgctgccc gggcaggaag cgctgggcgt ggaccagttg cagggcaact tcctgcgctt
      841 gcggttggaa ggctacaccc tcaaatggga gcgccatacc gagttcaccc gctactcgct
      901 ggtgcagccg ctgccagagc atgctgcgct gggggccaaa gagcccgagt tgttgtcgtc
      961 tatcgtcctg ccgcccgact ggttgcgcag cattcccgga cgcaccattg ccgccatcca
     1021 gctggccatg gtgcatggcg accctggcga tacccagaac accctggccc tggcccgcga
     1081 ctggattggc gagtatccgc tggtggcctc cgtcatgggg aacaacagcc actccctggc
     1141 ggtcacggat ttcattctcc ggcgggacgg cttcgagcgc atcctggtga tcgcgccgcc
     1201 cgagacatcg cgtacgcggg cggggcgggt gtcacaacgc ctgctagagc tggaaaccta
     1261 ccggctgatg gcattgcggg gtttgccggt ggccaagggc ctcggtccca tgctctccca
     1321 ggcagagagt gcgctggcgg acattaccgc gcagctggag aacaagtcca catccgacca
     1381 ggagctgctc gataccctgg tgcacctggc tgcgcgcgtg gagcgcgcca cggccgaaca
     1441 catttaccgt ttttctgcca cgcgggccta tgacacgctg gtggcccagc gcattgtgga
     1501 gttgcgcgaa aaggcggtgc ccggcaccca gaccattggc gaattcatgc aacggcgtct
     1561 ctcgcctgcc atggccacgg tggcggccac cagccagcgg ctgacttcgc tgtcggaacg
     1621 ggtggcgcgc accagcgcac tgctgcgcac acgggtggac attgccaccg aagtgcagaa
     1681 ccagcagttg ctggaaaagc tgacccgcgg gcaggaaatg cagctgcggc tgcagaccac
     1741 cgtggaaggc ctgtcgattg cggccatttc gtactacgtg gtgagcctgc tggtgtatgg
     1801 cggcaaggcg cttaaggggg cgggcgtgcc ggtcaatccg gaagtggcag ccggtgcact
     1861 gataccgctg gtgttgtggg gcgtgtggcg cgcaacccag cgcatccacc gcaagatcca
     1921 ccacggtgca tcaggccgct gatttggccc ggtatcaggc ctgtatggcc tgtggggtct
     1981 gggtcaggta gaacagcgca agcttgtctt cgcgcgtgag gttctggaac gccatgccga
     2041 tgaaatgtgc atcctcgtgg gtgcccttgt tgacgtggca gaccttggcg tccaggatca
     2101 gctgcatcgg accaccatcg accgtgacat ccatcaccac cttcagcggc gcgccgacgg
     2161 cggccaggtt cgagctggcc ttgatcatgg caccggccac gctgatgtct accagtgcca
     2221 cttccacatc gccaccgggc tcgcgcactt gggtgggcca ggaagtct
//
LOCUS       DAOSJM010000383         2261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_41272_length_2261_cov_3.594742, whole genome shotgun sequence.
ACCESSION   DAOSJM010000383 DAOSJM010000000
VERSION     DAOSJM010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41272_length_2261_cov_3.594742"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..764)
                     /locus_tag="PKX82_11595"
     CDS             complement(21..764)
                     /locus_tag="PKX82_11595"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOF52943.1"
                     /translation="MAVVFEFGEQMDGIPVRVVNERAVRAAAGLLFVPAFVAFMNAML
                     LGNFQPTRVFIVLFLVDMAVRLFINPRWSPSLIAGQWIVRSQQPEWVGAPQKRFAWGL
                     GLLLAIAMFFLMVVNQVIGPINMLVCGTCLVLMFFESAFGICLGCKLYNLFNREAAQL
                     CPGGACAIPAKDVKGLGWAQRLVLLGFAGLIVVTSQWVRSTAPAGAPVANAPLAAPQD
                     AAEAERCKVPEFAKAIGHEAQWKLHNHCP"
     gene            850..1554
                     /locus_tag="PKX82_11600"
     CDS             850..1554
                     /locus_tag="PKX82_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOF52944.1"
                     /translation="MIPIKTESAWRGTSDCRNCGIRDMVLFADLNEQDFGMIHAPIDD
                     LEIRAGTELFQEAIAAQGIYTLRTGLIKLVRATSDGRQRIVRVLRPGDVAGLEALAHS
                     RYDTDAVALTDISVCRIPLAVIQALGAQSPRLHRRLMEKWSRTLKEADDWLADLNFGT
                     ARNRVCNFVLKMRSSTDAQYVTLFAREDMGAMLDLKMETVSREISALVREQTLEPLDK
                     NGRAYRIVNIERLQVA"
     gene            1675..1893
                     /locus_tag="PKX82_11605"
     CDS             1675..1893
                     /locus_tag="PKX82_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52945.1"
                     /translation="MLFLSWESTQKNVPAFEQIAEDYARYRNMPSAEAELAEKLRQGH
                     LFGGVRAHQRLSEETARALVRWLISGGH"
     gene            complement(1911..2054)
                     /locus_tag="PKX82_11610"
     CDS             complement(1911..2054)
                     /locus_tag="PKX82_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3149 domain-containing protein"
                     /protein_id="HOF52946.1"
                     /translation="MKLLTDLFSTDYGLMSISGIFFMLGMGVFFLRFFSRKMEEDARA
                     AGK"
BASE COUNT          509 a          657 c          648 g          447 t
ORIGIN      
        1 tgatttcggg agcgcccgga ctatggacag tggttatgca gtttccactg ggcttcgtgg
       61 ccgatggcct tggcaaattc agggaccttg caacgctcgg cctcggcagc atcctggggc
      121 gctgccagtg gtgcgttggc cacaggcgcc ccggctgggg ccgtgctgcg cacccattgc
      181 gacgtgacca cgatcagacc ggcaaaaccc aaaagcacca ggcgctgcgc ccagcccagc
      241 cctttgacgt cttttgcggg aatcgcgcat gcgccgccgg ggcacagttg tgctgcttcg
      301 cggttgaaca ggttgtagag cttgcagccc aggcagatgc caaacgctga ctcgaaaaac
      361 atcaacacca ggcaagtgcc gcacaccagc atattgatag gcccaatgac ctggttgacc
      421 accatcagaa agaacatggc aatggccagc agcagcccca ggccccaggc aaagcgtttt
      481 tgcggggccc ccacccactc gggttgctgc gagcgcacga tccattgccc ggcgatcaga
      541 ctgggcgacc agcgcgggtt gataaaaagc cgcaccgcca tgtccaccag aaacagcacg
      601 ataaacacac gcgtgggctg gaagttgccc agcagcatgg cgttcatgaa cgcaacaaat
      661 gcgggaacaa acagcaaacc tgctgcagcg cgcaccgcac gctcattgac gacccgcacg
      721 ggaattccgt ccatttgctc accaaactca aataccaccg ccatgggaat ttccttgctg
      781 atgcgacaat tgcatccgtc tgcacgaata tagtcgagtg ccagaacccc tcttccaagc
      841 actgcctcca tgatccccat caaaaccgaa tccgcgtggc gaggcacatc cgactgccgc
      901 aattgcggca tccgcgacat ggtgcttttt gctgacctga acgagcagga tttcggcatg
      961 atccatgctc cgattgacga tctggaaatc cgcgcaggaa ccgagctgtt tcaggaagcg
     1021 attgctgccc agggtatcta taccttgcgt accgggctga tcaaactggt gcgcgccacc
     1081 agtgacgggc gtcagcgcat cgttcgggtt ttacgtcctg gtgatgtggc cgggctcgaa
     1141 gccctggccc attcacggta cgacaccgat gccgtggcgt tgacagacat ttcggtgtgc
     1201 cggattcccc tggcggtgat tcaggctttg ggtgcgcagt cacccaggct gcatcggcgg
     1261 ctgatggaaa agtggtcgcg taccctgaaa gaagctgacg actggctggc cgatctgaat
     1321 ttcggtactg cgcgcaaccg cgtgtgcaac tttgtactca agatgcgcag tagcaccgat
     1381 gcgcaatacg tgacgctgtt tgcgcgcgag gacatgggcg ccatgctcga cctgaagatg
     1441 gagacggtca gccgcgaaat cagcgccctg gtgcgcgagc agacactgga gccgctggac
     1501 aagaacggca gggcctaccg tattgtcaat atcgaacggc tgcaagtagc ctgacgagga
     1561 ttgtggagct gcgcatatgg tgatgaaaca gctgatcagc tatgtcgcaa taggggtagt
     1621 tgcaagctac tcattgccag cgcatgccaa tgcccagctg gcattggaca agggatgcta
     1681 ttcctgtcat gggagtccac ccaaaaaaac gtcccagcct ttgagcagat tgccgaggac
     1741 tatgcccgtt accgcaacat gccctcagca gaagctgaac tggccgagaa gctacggcaa
     1801 gggcatctct ttggtggcgt ccgggcgcac cagcggttgt ctgaagaaac tgcccgtgcg
     1861 ctggtccgct ggttgatcag tggcggccac taagtccgcg caatttcgtc ctattttccg
     1921 gctgccctgg catcttcttc catcttgcga ctgaagaagc ggagaaagaa aacgcccatg
     1981 cccagcatga agaaaatgcc actaatgctc atcagcccat agtcggtaga aaaaaggtcc
     2041 gtcagtaatt tcatggggta ctcctttggt tggatctgca cagtgtgctt cctcaggcac
     2101 atctggcgat tgatattcat caatgctcgg catgcgccga aagaggattg gctgtctatt
     2161 gatgcagcgc aataccccgg tctgtaggga aattggcagc caggcctttt caggtccaag
     2221 tgcacccaac atcgggctgg cagaatcggg ccatgcttga a
//
LOCUS       DAOSJM010000384         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_41350_length_2257_cov_4.036331, whole genome shotgun sequence.
ACCESSION   DAOSJM010000384 DAOSJM010000000
VERSION     DAOSJM010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41350_length_2257_cov_4.036331"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1483)
                     /locus_tag="PKX82_11615"
     CDS             complement(173..1483)
                     /locus_tag="PKX82_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOF52947.1"
                     /translation="MSIKSLLMPLVTQRLFSRERLLSRRARAERQRQSAGLPHAVHYF
                     HQVDDPYSALVAACLKDLQTRYAITLIPHLVSPPEAAAAPERGRLVAYSRRDAQLLAA
                     RHGLQYQDLGVQPTPAQVDTANRQLVAAMEDGVFIDVAPEVSRRLRQIPHDGQPNPLW
                     PEARLASSEATASHLQASDALRSQWGHYLGATFYYGGEWYWGIDRLYHLEQRLQELGA
                     QKAGVQGYLFPPGDDLAADTPLQNAPAIDFFFSLRSPYSAIVARRVFDLGRRTGAPVR
                     LRFLLPMVMRGLPVPKAKRQYIAHDAAREAHVRGIPFGRLNDPVGRPTERGLALIPLA
                     ESRGLGEAYLVSFMQGVWAEGLDAGSDRGLRTIAERAGLSWTDAQAALKDEAWRATAE
                     ANREAMFALGLWGVPSFHVGDTAVWGQDRLWAVQDALFAQAQSA"
     gene            complement(1488..1781)
                     /locus_tag="PKX82_11620"
     CDS             complement(1488..1781)
                     /locus_tag="PKX82_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52948.1"
                     /translation="MADYKIQRTMGPLDLINHLLNFVAPALFVGTALACVAPVFMGNR
                     PVARVVVAQAAINSIAGVVALSVGLWFFGRDGKMASYAAMLLGCALSQGLLRK"
     gene            1789..>2257
                     /locus_tag="PKX82_11625"
     CDS             1789..>2257
                     /locus_tag="PKX82_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOF52949.1"
                     /translation="MAVWALGINHNTAPLDMRGRFAFAMDQIAPHLEGLRQSMERPPE
                     AAIVSTCNRTEIYCAGDESDLERTLAWLAKSGGVSATELRGHTYQMEDGQAARHAFRV
                     ASGLDSMVLGEPQILGQLKDAVRAADAAGALGTTLSQLFQRSFAVAKEVRTSTE"
BASE COUNT          462 a          747 c          666 g          382 t
ORIGIN      
        1 cgagctgacc gagttgaccg ctcaggtcgt tgaagtcgtc ggcaacgatc accacaatat
       61 tgggtcgcct gggttctgca gccccggcag gcactgcact cccaaaaatg gacagaagca
      121 acagggcaaa gggaaatcgg gacatgctgg tttctcgctg aagcttctgc catcaggccg
      181 attgcgcctg cgcaaacagg gcatcttgta ccgcccacag acgatcctgc ccccacacgg
      241 cagtatcgcc gacatgaaaa gaaggcaccc cccacagtcc cagggcaaac atggcttcgc
      301 ggttggcctc ggcagtcgcg cgccaggctt cgtctttcag cgccgcctgc gcatcggtcc
      361 aggacaaacc cgcgcgctcg gcaatggtac gcaagccacg gtcactgccc gcatccagac
      421 cctcggccca caccccttgc atgaatgaga ccagataggc ctcgcccagt ccccggcttt
      481 ccgccagcgg aatcaaggcc agtccgcgct ccgtcggtcg acccactggg tcattcaaac
      541 ggccaaacgg tatgccccgc acatgtgcct cgcgcgcagc gtcatgggca atgtactggc
      601 gcttggcctt gggcaccggc aagccccgca tgaccatggg caacaggaaa cgcaggcgca
      661 ctggcgcccc ggtgcggcgc cccaggtcaa acacacgacg ggccacaatg gcggaatacg
      721 gactgcgcag ggagaagaaa aaatcaatgg ctggcgcatt ctgtaatggg gtgtctgctg
      781 ccaggtcgtc gcctggcgga aacagatagc cctgtacgcc ggccttctgc gcaccgagtt
      841 cctgcaagcg ctgttccaga tggtaaagac ggtcgatgcc ccaataccac tctccgccgt
      901 aatagaaagt cgcccccaga tagtggcccc actgcgagcg caacgcgtcg gatgcctgca
      961 ggtggctcgc tgtggcctcg ctggaggcca accgggcctc tggccaaagc ggatttggct
     1021 gtccatcgtg cgggatttgc cggagtcggc gcgaaacctc gggtgccacg tcaataaaca
     1081 ccccatcctc catggcagcc accagttgcc ggttggccgt atcaacctgt gctggcgttg
     1141 gctgcacgcc caggtcctgg tactgcaaac cgtgacgggc ggccagcagc tgtgcgtccc
     1201 tgcggctgta ggccaccagc cggccgcgct cgggcgccgc cgcggcctcg ggcgggctga
     1261 ccagatgggg aatcagcgtg atggcgtagc gggtttgcag gtccttcaga caggctgcta
     1321 ccaacgcact gtaggggtca tcgacctggt ggaagtagtg caccgcatgc ggcaacccgg
     1381 ccgactgacg ctgccgctca gcccgggcgc gtcggctcag cagtcgttcg cgcgaaaaca
     1441 gccgctgggt gaccagtggc atgagcaggc tcttgatcga catggcatca tttcctcaac
     1501 aacccctgac tcagcgcaca gcccaaaagc atggcggcat acgacgccat cttgccatct
     1561 cttccgaaaa accacagtcc aacagacaac gccactacac cagctataga atttatagct
     1621 gcttgcgcaa ctaccacgcg ggctacaggc ctgtttccca taaaaacggg agccacacat
     1681 gccaatgccg tgccgacgaa cagcgcggga gccacgaagt tgaggaggtg gttgatcagg
     1741 tctaatgggc ccatggtgcg ctggatttta taatcggcca caacatatat ggcagtctgg
     1801 gcattaggca tcaatcacaa caccgcaccg ctggatatgc gcggtcggtt tgcgttcgcc
     1861 atggaccaga tcgcaccgca cctcgaaggc ttgcgccagt cgatggagcg gccgcccgaa
     1921 gcggccattg tttccacctg caaccgcacc gaaatctatt gcgctggcga cgaatccgac
     1981 ctcgaacgca ctctggcctg gctggccaaa tccggcggcg tttctgccac tgaactgcgc
     2041 ggccacacct accagatgga agacgggcag gccgcccgcc acgccttccg cgttgccagt
     2101 ggcctggact ccatggtgct gggcgagccg caaattctgg gccagctcaa ggacgctgtg
     2161 cgtgccgccg atgcggccgg tgccctgggc accacactgt cccaactgtt ccagcgctcg
     2221 tttgccgtgg ccaaggaggt gcgcacctcg accgaaa
//
LOCUS       DAOSJM010000385         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_41351_length_2257_cov_3.662579, whole genome shotgun sequence.
ACCESSION   DAOSJM010000385 DAOSJM010000000
VERSION     DAOSJM010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41351_length_2257_cov_3.662579"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..307
                     /locus_tag="PKX82_11630"
     CDS             65..307
                     /locus_tag="PKX82_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52950.1"
                     /translation="MKKALLALVLLAQAATFSIATETDKETREDIAKHRAMAAAHEAA
                     AKCREAGKGEDVCNKELQAACKGLAIGKFCGMKHEH"
     gene            419..892
                     /locus_tag="PKX82_11635"
     CDS             419..892
                     /locus_tag="PKX82_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3299 domain-containing protein"
                     /protein_id="HOF52951.1"
                     /translation="MGGAQAQLSSPMGPGGAVPAGSGAGVHSPNSPFAPLQERNDVLP
                     WSLLTSIKTKTEKNRILPVFTPAVMALNAKSQRVQGFMMPLEPGEKQKHFLLSSVPLS
                     CSFCVPGGPESMVEVKTKTPVKYSLEAVVVEGKFAVLQDDPYGLYYRITDAVPVK"
     gene            914..1867
                     /locus_tag="PKX82_11640"
     CDS             914..1867
                     /locus_tag="PKX82_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOF52952.1"
                     /translation="MDHPVVSSLVPVVLLIVVGLVAGRLQLIRQEAVRDLSNLVFLVL
                     TQALLFRTMSTVHIEQLDFRPVWLYFAVAAGLFTVLLVVQGTNSRSAVLALSGTFSNT
                     VMIGIPLIGLAYGKDALVLLFTLISLHALVLLTMATVVLELLQAREQVQAGLAPERHR
                     LATIGLAIRNAVVHPVPMPIIAGLLFAQTGWTLPEVVDRPLLLLGNAFGPVALVLVGV
                     TLSQAHVGREFKGALALAGIKTLLHPLLMALAGWAGGLRGPSLVVLVVAASLPIGANV
                     FLFSQRYQKAQDLVTAGVAVSTVLALFSVTLVMTLVPLIAN"
     gene            complement(1889..>2257)
                     /locus_tag="PKX82_11645"
     CDS             complement(1889..>2257)
                     /locus_tag="PKX82_11645"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HOF52953.1"
                     /translation="PFAALQFHWVELERVVRIEGRVEKVSAEESDAYFQSRPLDSRVG
                     AWASPQSQVIPGRTVLVTNAAKYAAQFLLHPPRPPHWGGYRLTPERWEFWQGRKSRLH
                     DRLRYSLQEEGSWLRERLAP"
BASE COUNT          366 a          680 c          739 g          472 t
ORIGIN      
        1 gctggaactg ctgcagaccc actagatgat ctctttttcc tccactccaa cctgaaggta
       61 atttatgaaa aaagcactcc tggcccttgt cctgcttgcg caagcagcta cgttttccat
      121 agcaactgaa accgacaagg aaaccaggga agacatcgcc aaacaccggg ccatggcagc
      181 cgcccatgaa gctgccgcca aatgccgcga ggctggcaag ggtgaagatg tctgcaacaa
      241 ggaactgcag gccgcctgca aaggtctggc gattggcaag ttctgcggca tgaagcacga
      301 gcattgagct ctatttccct gagacggcgc gtgcgtgagg ccacgcttta agataagcct
      361 gttggaggtt cccatgaaga cagtctttcg cgttgtaccg gtcctgctcg ccctgtcgat
      421 gggcggtgcg caggcccagc tcagttcccc catggggccc ggtggggcgg tgccagcggg
      481 gtccggtgcc ggggtgcaca gcccgaacag cccgtttgcg ccactgcagg agcgcaacga
      541 cgtgctgccc tggtcgctgc tgacgtccat caagaccaaa accgagaaaa accgcatcct
      601 gccggtgttc acaccggccg tgatggcgct caatgccaag agtcagcgtg tgcagggctt
      661 catgatgccg ctggagccgg gcgaaaagca gaagcatttc ctgctcagtt ctgtgcccct
      721 gtcctgttcc ttctgtgtgc ccggtgggcc cgaaagcatg gtggaggtca agaccaaaac
      781 gccggtgaaa tattcgctgg aagccgtggt cgtggaaggc aagttcgccg tgttgcagga
      841 tgacccttac gggctctact accgcatcac cgatgcggtg ccagtgaagt aggttgcaga
      901 tttgctgatg ccgctggacc atccggtcgt ttcttcgctg gtgccggtcg tcctgctgat
      961 cgtggtgggg ctggtggccg ggcggctgca gctcatccgc caggaggcgg tgcgcgacct
     1021 gtccaatctg gtgtttctgg tgctgacgca ggcgctgctg tttcgcacca tgagcacggt
     1081 gcacatcgag cagctggact tccgccccgt atggctgtac ttcgcggtgg cggccgggtt
     1141 gtttacggta ctgctggtgg tgcaggggac caacagccgg tcggcggtgc tggccttgtc
     1201 gggcacgttc agcaacaccg tgatgatcgg cattcccctg atcggtctgg cctatggcaa
     1261 ggacgcgctg gtcctgctct tcacactgat ttccctgcat gccctggtgc tgctgaccat
     1321 ggcgacggtg gtgctggaat tgttgcaggc gcgcgaacag gtgcaggccg ggctcgctcc
     1381 cgagcgccat cgcctggcca ccattggcct ggccatccgc aatgcggtgg tacatccggt
     1441 gcccatgccc atcatcgccg ggctgttgtt tgcccagacc ggttggacac tgcccgaggt
     1501 ggtggaccgc ccgctgctgt tgctgggcaa cgcgtttggc cccgtcgcgc tggtgctggt
     1561 gggagtcacg ctgtcgcagg cgcatgtggg acgtgagttc aagggtgcgc tggcgctggc
     1621 cggcatcaag accctgctgc acccgttgct gatggccctg gccggatggg ctggcgggct
     1681 gcgtgggcca tcgctcgtgg tgctggtggt ggctgcgtcg ttgccgatcg gtgccaatgt
     1741 gttcctgttt tcgcagcgct accagaaggc gcaggacctg gtgacggctg gcgtggcggt
     1801 ttccaccgtg ctggccctgt tcagcgtgac gctggtgatg acgctggttc cgctgatcgc
     1861 gaattgaccg gtcagcttgg ggtagccgtc agggtgcgag ccgctcccgc agccagcttc
     1921 cttcttcctg caggctgtag cgcagccggt cgtgcaggcg gctcttgcgg ccctgccaga
     1981 attcccagcg ttccggtgtg aggcggtagc ccccccagtg cggcggacgc ggtgggtgca
     2041 gcagaaactg cgcggcgtat ttggcggcgt tggttaccaa tacggttctg ccaggaatga
     2101 cctggctttg cggcgaagcc caggcgccca cccgtgaatc cagcggccgg ctctggaaat
     2161 aggcgtcgct ttcttctgca ctgacctttt ccacccggcc ttcaatccgc accacccgct
     2221 ccagttccac ccaatggaat tgcagcgcgg caaacgg
//
LOCUS       DAOSJM010000386         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_41386_length_2256_cov_3.609269, whole genome shotgun sequence.
ACCESSION   DAOSJM010000386 DAOSJM010000000
VERSION     DAOSJM010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41386_length_2256_cov_3.609269"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PKX82_11650"
     CDS             <1..388
                     /locus_tag="PKX82_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52954.1"
                     /translation="GFTSAPPVSTSYIYNSAASLSTITGSWTGSTLFGEAGTMVIASN
                     GTFTASFTGSYVGTCTATGTVQPRASGKNVFNVTMTFGAAPCGLPGTTATGIALSYLL
                     INGQRQLIAAATTSSKANGAAFFAAR"
     gene            complement(425..549)
                     /locus_tag="PKX82_11655"
                     /pseudo
     CDS             complement(425..549)
                     /locus_tag="PKX82_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930198.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(602..871)
                     /locus_tag="PKX82_11660"
     CDS             complement(602..871)
                     /locus_tag="PKX82_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOF52955.1"
                     /translation="MKCPTCPDSTLVMTDRQGVEIDYCPACRGIWLDRGELDKLLERS
                     ATVSAPPPAPVSQSRKQPDFEDSDYRGGQRYSQHRKKSWLHEIFD"
     gene            complement(885..1241)
                     /locus_tag="PKX82_11665"
     CDS             complement(885..1241)
                     /locus_tag="PKX82_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOF52956.1"
                     /translation="MQIIFESRDADGSQMREVSVERVRFTLRRLSALVPRAKVQFSDV
                     NGPRGGVDKRCQVELSTDAAGTVVIASLARDWRTALDHSLRRATRVLTRSLHRNNKPV
                     RGRTARLALDSQSQPS"
     gene            1342..2226
                     /locus_tag="PKX82_11670"
     CDS             1342..2226
                     /locus_tag="PKX82_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOF52957.1"
                     /translation="MSAPFNYKHLYYFWVVAKEGGISRAADKLDMAVQTVSAQVRELE
                     RSLGYALLKPAGRGLVLTDAGLAAMQQADQIFQLGEKLPALVRDAVSAPAVRLAVGIS
                     DGLPKLVVRHLLQPVIPEPHLRLLCHEGEFDELLGDLALHRLDVVLSDRPAPTNPNIK
                     LYSHALGSSTIAWYGTADMVKAASSDFPRSLADVPMLLPTGHTAVRARLDHWFEQRAI
                     RPRIVGEFEDSALLKTFGASGMGIFPAAEWANDDLLAHYAVQRLGPCEGVNEHFFAIG
                     TEKKVHHPLVQRLLQPAP"
BASE COUNT          465 a          661 c          669 g          461 t
ORIGIN      
        1 aggcttcaca agtgctcccc ctgtcagcac cagctacata tacaactctg ccgcttcact
       61 ctcgaccatc accggcagtt ggactgggtc gaccttgttt ggcgaggcag gaaccatggt
      121 gattgcgtcc aatggcactt tcactgcgag ctttaccgga agttacgttg gtacttgcac
      181 agcaaccggc acagtacaac cgcgagcatc gggaaagaat gtttttaacg tgactatgac
      241 tttcggtgcg gcgccatgtg gacttcccgg tactacagcg acaggaatag cgctcagtta
      301 tctgctgatt aatggacaac ggcaactgat tgccgcagcc acaacatcca gcaaggccaa
      361 tggcgcagcc tttttcgcag cacgatagac atgagcgagc gccgcatgac gcggcgccgt
      421 gtaccaacac acagctgacc gtgctacagg agacgcccag cgctgcggca atgcatacct
      481 gagtcagtcg gcggtgacgt agctccacga tggccaatgc cttttgggat ctatgaatcg
      541 gcggctcaca gcaagagatc gttccatccg agccgcaggt agcggctgtc cggcctaaat
      601 ctcaatcaaa aatctcgtga agccaggact tcttgcggtg ctggctgtaa cgttgcccac
      661 cacggtaatc cgagtcttca aagtcaggct gcttgcgact ctgcgacaca ggcgctgggg
      721 gcggtgcaga aacagtggct gaacgctcca gcagtttgtc gagttcaccg cggtcgagcc
      781 agatgccgcg gcaggccggg cagtagtcaa tttcgacacc ctgtcgatcg gtcatcacca
      841 gggtggaatc ggggcaggtg gggcatttca tggtgttggt tgctttatga cggttgagat
      901 tggctatcga gggcaagtcg agcggtgcgt ccacgcaccg gtttgttgtt gcgatgcagg
      961 ctgcgcgtca gcacgcgggt agcacgccgc agggagtggt ccagtgcagt gcgccaatcg
     1021 cgggccagag aggcgatgac cacggtgcct gcagcgtcgg tactcagctc gacctggcaa
     1081 cgcttgtcca caccaccgcg gggtccattc acgtcggaga actgcacctt ggcgcgtggc
     1141 accagggcac tgagacggcg cagcgtaaaa cggacacgct caaccgacac ctcgcgcatc
     1201 tggctaccgt cggcgtctcg ggactcaaaa atgatttgca ttgcattgtc tccagtgggt
     1261 gaaagaagac ccgaagatag gagcccaatt gattcttaaa aagcagataa tttctcatcc
     1321 agactcttaa aaaacctgat catgagcgcc cccttcaact acaagcacct ctattacttt
     1381 tgggtcgtgg ccaaggaagg cggcatctcg cgggctgccg acaagctgga tatggcggtg
     1441 caaaccgtca gtgcccaggt gcgtgagctg gaacgatcgc tgggctacgc gctgctcaag
     1501 cccgctggac gcggattggt gttaactgac gcgggcctgg ctgccatgca gcaggcagac
     1561 cagatttttc aactgggcga gaaactgcca gctttggtac gcgacgccgt cagcgcaccc
     1621 gcggtgcgcc tggccgttgg catctcggat ggcctgccca agctcgtagt tcgccacctg
     1681 ttgcagccgg taattcccga gccccatttg cgtctgctgt gccatgaggg cgagtttgac
     1741 gagctgctgg gtgaccttgc cttgcaccgt ctggatgtgg tgctgtcgga ccggcctgcg
     1801 ccaaccaacc ccaatatcaa gctgtacagc catgccctgg ggtcctcgac cattgcgtgg
     1861 tacggcaccg cagacatggt caaggccgcc agcagcgact ttccccgcag cctggccgat
     1921 gtgcccatgc tgctccccac aggccatacg gccgtgcggg cacggctgga ccactggttt
     1981 gagcagcgcg ccatccggcc gcgcattgtg ggggagtttg aagacagcgc attgctgaaa
     2041 acctttggcg ccagcggcat gggaatcttt cctgctgccg agtgggctaa cgacgatttg
     2101 ctggcccatt acgcagtgca gcgtttgggc ccgtgcgagg gtgtgaacga gcacttcttt
     2161 gccatcggca ccgaaaagaa agtgcaccat ccactcgtgc agcgtttact gcagccagca
     2221 ccgtaactcc gtttcaaacg ctgaggcttt gccttt
//
LOCUS       DAOSJM010000387         2245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_41687_length_2245_cov_4.142922, whole genome shotgun sequence.
ACCESSION   DAOSJM010000387 DAOSJM010000000
VERSION     DAOSJM010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41687_length_2245_cov_4.142922"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /gene="slmA"
                     /locus_tag="PKX82_11675"
     CDS             complement(<1..448)
                     /gene="slmA"
                     /locus_tag="PKX82_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HOF52958.1"
                     /translation="MPDAPHTQPIEVQHSDAQAAEAESPSTRKRPKPGERRIQILQTL
                     AAMLEQPSGDRITTATLAARLEVSEAALYRHFASKAQMFEGLIEFIEQSVFTLVNQIV
                     EREEAAGGDAQLVGARQASRIITMLMQFAERNPGMARVMVGDALVFE"
     gene            complement(488..1921)
                     /locus_tag="PKX82_11680"
     CDS             complement(488..1921)
                     /locus_tag="PKX82_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOF52959.1"
                     /translation="MTAGLQRRLLVLLLLPLLLLAALNTWFDYRSADSAALQQDRQLR
                     SLVPLLADSVVARGAGLQDPPVLLTAPAIDEFLGQRQGVSAFAIVGFDGRVMAGAEWL
                     TGLPPTTHEAEFSSEEFDGTTYRIVSQRMQTAAGELTMRLADGSDPRQQWLRLLWLKV
                     ALPNLVLAVAGFFAVNWAVRRALRPLIELKEAVEHRSPRDLTAIDEASSPDEVRPLVA
                     SLNRLFDLVNAQAENQRRFVADAAHQLRTPLAGLQAQVEAWAQAVSSERYKQNDAPAH
                     VKPASEAITLGADQVYKLRQATRRTSQLASQLLALSRVDARSANAAPMQRVELKQLCE
                     VVLEGHLDIAAAKGIDLGLDATVVHADGYEWLLRELLTNLVDNAVRYTQQGGCVTLRC
                     GHMLEDQRPFLEVEDDGPGVPVAERGRVLERFYRVPGTGGEGNGLGMAIADEIAHLHH
                     SVLELHGGPNGRGLRVLLRLPESTPNA"
     gene            complement(1936..>2245)
                     /locus_tag="PKX82_11685"
     CDS             complement(1936..>2245)
                     /locus_tag="PKX82_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOF52960.1"
                     /translation="RQACIRSADSGATVLIELGPREWTVMEYLLLQSPKPANKDKLLQ
                     ALTGWDREITPNAVEVYISRLRGKLEPHGIALRAIRGFGYRLELMGGIASSSDAASGV
                     "
BASE COUNT          403 a          741 c          686 g          415 t
ORIGIN      
        1 tctcaaacac cagtgcatcg cccaccatga cgcgggccat gcccgggttg cgctcggcaa
       61 actgcatcag catggtgatg atccggctgg cctggcgtgc gcccaccaac tgcgcgtcac
      121 cgcctgcggc ctcttcccgc tccacgatct ggttgaccag cgtgaagacc gactgctcga
      181 tgaattcaat caggccttcg aacatctggg ccttgctggc aaagtggcgg tacagcgctg
      241 cttcgctgac ttccagacgt gccgccagcg ttgccgtggt gatgcggtcg ccagagggtt
      301 gctccagcat ggctgcgagc gtctgcaata tctggatgcg ccgctccccg ggtttggggc
      361 gcttgcgtgt ggagggagac tctgcctcgg ccgcctgcgc gtcggagtgc tgtacttcga
      421 tgggttgcgt gtgtggtgcg tctggcatgg ttcgcaagcg gtggttttaa atcattctcg
      481 cacagggtta tgcgtttggg gtcgactccg gcagcctcag caaaacacgc agtccacgtc
      541 cgttggggcc gccatgcaac tccagaacgc tgtggtgcag atgggcaatt tcatcggcaa
      601 tggccatccc caggccattg ccttccccgc ccgtgcccgg gacacggtag aagcgctcca
      661 ggacccgccc gcgctctgcc accggcacgc cggggccatc gtcctcgacc tccaggaaag
      721 ggcgctggtc ctccagcata tgaccgcacc gcaaggtgac gcaaccgcct tgctgcgtgt
      781 aacgcactgc gttatcgacc agattggtca gcagctcacg cagcaaccat tcgtagccat
      841 cggcgtggac gacggtggcg tccagcccca gatcaatccc tttggccgca gcgatgtcca
      901 gatgcccctc cagaaccact tcacacagct gcttgagctc cacgcgctgc atcggggccg
      961 cattggcact gcgtgcatcc accctggaaa gcgcaagcaa ctggctcgcc aactgcgacg
     1021 tacggcgggt ggcctggcgc agtttgtaca cctggtccgc tcccaaagta atagcttccg
     1081 acgcaggttt cacgtgggct ggagcatcat tttgcttgta acgttcggaa ctgaccgcct
     1141 gggcccatgc ttccacctgc gcctgcagac ccgccagcgg cgtgcgcaac tggtgcgccg
     1201 catcggcgac aaaccggcgc tggttttcgg cctgggcatt gaccagatcg aagagacggt
     1261 tcagcgacgc caccaggggc cgcacctcgt cgggtgacga ggcttcatcg atggcggtca
     1321 gatcccgcgg cgaccggtgt tcgaccgcct ctttcagctc tatcagtggt cgcaaggcgc
     1381 gccgtaccgc ccagttgacg gcaaagaaac ctgcgaccgc cagcaccaga ttgggcaagg
     1441 cgaccttgag ccacagcagg cgcagccatt gttggcgcgg gtcagacccg tcggccaggc
     1501 gcatcgtgag ttcgccggct gcggtctgca tgcgctggct gacgatgcga taggtggtgc
     1561 catcaaactc ttcgctggag aactcggctt cgtgcgtggt gggcggcagt ccggtcagcc
     1621 actcagcccc ggccatcacc cgcccgtcaa accccacgat ggcaaaagcc gagacgcctt
     1681 gccgctggcc cagaaactcg tcaatcgcag gggccgtcag caacacgggg ggatcctgca
     1741 atccagcacc acgggcgacc actgaatcgg ccagcagggg aaccaggctg cgcagctgcc
     1801 ggtcctgttg caatgcggcg ctgtccgcag accggtagtc aaaccaggta ttgagggccg
     1861 ccagcaacag cagtgggagc aagagcagca caagcaagcg gcgctgcagt cctgccgtca
     1921 ttccggccgt gtttttcaca ccccggacgc cgcgtcactg gatgacgcga tgccccccat
     1981 cagctccagt cggtacccga atccgcggat ggcgcgcaat gcaatcccgt ggggttccag
     2041 ctttccgcgc agacgcgaaa tatagacctc caccgcattg ggtgtgattt cccggtccca
     2101 gcccgtcagc gcctggagca gcttgtcttt attggcgggt ttgggagact gcagcaacag
     2161 atactccatg actgtccatt cgcgtggccc cagctcgatc agtaccgtgg cgcctgaatc
     2221 tgcactgcga atgcaggcct ggcgc
//
LOCUS       DAOSJM010000388         2237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_41902_length_2237_cov_3.838680, whole genome shotgun sequence.
ACCESSION   DAOSJM010000388 DAOSJM010000000
VERSION     DAOSJM010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41902_length_2237_cov_3.838680"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PKX82_11690"
     CDS             <1..229
                     /locus_tag="PKX82_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52961.1"
                     /translation="EIAMAIPLGMLGGYGLARGIVQMIKTDEFYFPFVIQPSTYATAA
                     ICVVLAGVVSALIVRRRIDTLDLVGVLKTRE"
     gene            246..1442
                     /locus_tag="PKX82_11695"
     CDS             246..1442
                     /locus_tag="PKX82_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOF52962.1"
                     /translation="MKKNRLWMAAGGVVVLALLVWAFSPSALEVETAVVSQGRFERSV
                     QEDGKTRLRDRFVVSTPLSGRVARIALKQGDVVEQGAVLATLWPASPALIDARTQQEQ
                     RERMGAMESALLRAGANIERAKAALDQAVADLKRNETLAKQGFVSPTQNESGRLTVRL
                     REKELESAVQDEHATRHELEQSRIAMRQFAPGTEGRQQRAWEVKAPIAGTVLKVNQQS
                     EGVVLAGTPLVELGDPSRMEVVVDVLTEDAAQIKPGTVATLSGWGGADALQARVRLVE
                     PAAFTKVSALGVEEQRVNAVLDITSAPDTWRALGDGFKVDVRVQVQVVDNALKVPVSA
                     LFPVGARSAMFVVEQGRAQQREVEVLARNGMEAWIKSDLKPGTTVIVYPPSKLASGDR
                     VTSTAH"
     gene            1456..1947
                     /gene="ybaK"
                     /locus_tag="PKX82_11700"
     CDS             1456..1947
                     /gene="ybaK"
                     /locus_tag="PKX82_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HOF52963.1"
                     /translation="MAKKDHVSETPATQLLKAHKVPFAEHPYEYLEHGGAQHSAQVLG
                     WDPFHVVKTLIMQDQDAKPLVVLMHGNRKVSTKNLARQIGAKSVEPCKPEVANRHSGY
                     LVGGTSPFGTRKTMPVYIEETILAIPKILINGGRRGYLVEIEPAVCVQLLGAKAVQCA
                     LLD"
     gene            1965..>2237
                     /locus_tag="PKX82_11705"
     CDS             1965..>2237
                     /locus_tag="PKX82_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOF52964.1"
                     /translation="MQAFYPALATVAAYLMGSLSFAVIVSSVMGLNDPRTYGSKNPGA
                     TNVLRSGSKAAAVVTLLLDAVKGWVPVVLVKWYGKPYGLEDGTLALV"
BASE COUNT          410 a          638 c          786 g          403 t
ORIGIN      
        1 ggaaattgcc atggccattc cgctgggcat gctcggcggc tacgggctgg cccgcgggat
       61 tgtgcagatg atcaagaccg acgagtttta ttttcccttc gtgattcagc catcgaccta
      121 tgccactgcc gccatctgcg tggtgctggc aggggtggtc agcgcactga ttgtgcgccg
      181 gcgcattgat acgctggatc tggtgggtgt actgaagaca cgggaatgac aggaaggaag
      241 tgaccatgaa gaaaaatcgt ctctggatgg ctgcgggcgg cgttgttgtg ctggctctgc
      301 tggtgtgggc cttttcgcca tcggccctgg aggttgaaac tgcagttgtc tcgcaggggc
      361 gctttgaacg ctcggtgcag gaggatggca agacccggct gcgcgaccgg tttgtggtgt
      421 ccacgccgct gtcggggcgc gtggcgcgca tcgcgctcaa acagggtgat gtggtggagc
      481 agggggcggt gctggccacg ctgtggcccg cttctcccgc actgattgac gcccgcaccc
      541 agcaggaaca gcgtgaacgc atgggtgcca tggagtcggc actgctgcgc gcgggtgcca
      601 atatcgaacg ggccaaagct gcgctggacc aggcggtggc cgacctcaaa cgcaatgaga
      661 ccctggccaa gcagggcttt gtctcgccca cgcaaaacga gtcgggtcga ctgacagtgc
      721 gcctgcgtga gaaggagctg gagagcgccg tgcaggacga acacgctacg cgccatgaac
      781 tggagcagtc gcgcattgcc atgcgccagt tcgcccccgg gaccgagggg cgccagcagc
      841 gtgcctggga ggtcaaggca ccgattgccg gcaccgtgct caaggtcaac cagcagagcg
      901 aaggcgtggt gctggccggc acgccgctgg tggagctggg tgaccccagc cgcatggagg
      961 tggtggtgga tgtcctgacc gaggacgcag cccagatcaa gccgggcacc gtggccacgc
     1021 tgtcgggttg gggtggcgcc gatgcattgc aggcgcgggt gcgcctggtg gagccggcag
     1081 ccttcaccaa ggtgtccgcc ctgggggtgg aagagcagcg tgtcaacgcg gttctggaca
     1141 tcacctccgc gccggacacc tggcgcgcgc tgggggacgg cttcaaggtg gatgtacggg
     1201 tgcaggtgca ggtggtggac aacgccctca aagtaccagt gagcgcactg ttcccggtgg
     1261 gcgcgcgctc cgccatgttt gtggtggaac agggccgcgc gcagcagcgc gaggtcgagg
     1321 tgcttgcgcg caatggcatg gaagcctgga tcaagtcgga cctgaaaccc ggcacgacgg
     1381 tcatcgtcta cccgcccagc aaactggcgt cgggtgaccg ggttacttcc acagcccatt
     1441 aggagaaagc cgccgatggc aaagaaggac cacgtttcgg aaaccccggc aacgcagttg
     1501 ctcaaggcac acaaggtgcc attcgccgag cacccctacg aatacctgga gcatggcggt
     1561 gcccagcaca gcgcgcaggt gctgggctgg gacccgttcc atgtagtcaa gacgttgatc
     1621 atgcaggacc aggatgccaa accgctggtg gtgctgatgc atggcaaccg caaggtctct
     1681 accaagaacc tggcgcggca gattggcgcc aagtcggtcg agccatgcaa gcccgaggtg
     1741 gccaaccggc acagcgggta cctcgtgggt ggaacctcgc cgtttggcac ccgaaagacc
     1801 atgccggtgt acatcgagga aaccattctg gccatcccga agatcctgat caatggcggg
     1861 cgccggggtt atctggtgga gattgaaccc gccgtgtgcg tgcagctgct gggtgcaaaa
     1921 gcggtacagt gtgcgctgtt ggattgaagg agtctggttt ggaattgcaa gctttctacc
     1981 ccgcgctggc cactgtggcg gcgtacctga tggggtccct ttcttttgcg gtgatcgtca
     2041 gcagtgtgat gggcctcaac gacccgcgca cctatggcag caagaatccg ggcgccacca
     2101 atgtgctgcg ttccggttcc aaggccgctg ccgtcgtcac gctgctgctc gacgccgtca
     2161 aaggctgggt gccggtggta ctggtcaagt ggtatggcaa gccctatggt ctggaagatg
     2221 gcacgctggc gctggtg
//
LOCUS       DAOSJM010000389         2233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_41999_length_2233_cov_4.968779, whole genome shotgun sequence.
ACCESSION   DAOSJM010000389 DAOSJM010000000
VERSION     DAOSJM010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41999_length_2233_cov_4.968779"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PKX82_11710"
     CDS             complement(<1..922)
                     /locus_tag="PKX82_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HOF52965.1"
                     /translation="MTFSKILIANRGEIACRVIRTARNLGYQTVAVYSDADRDAPHVL
                     LADEAVYIGGSPAAESYLKFDALLDAARKTGADALHPGYGFLSENAAFAQACVDANVV
                     FIGPPPSAIEAMGDKALAKRRMLDAGVPCAPGYLGADQSDAVLIAEAEKLGFPLLVKA
                     VAGGGGRGMRLVRHFAELQQGIEGARREATSAFGDGTLMLERLIDNGRHIEIQVFADA
                     HGNAVYLGERDCTAQRRRQKVIEEAPSPVVSPAMRAAMGKDAVAAALAVGYRGAGTVE
                     FIVDDKLNHYFLEMNTRLQVEHPVTECITGY"
     gene            complement(919..1737)
                     /locus_tag="PKX82_11715"
     CDS             complement(919..1737)
                     /locus_tag="PKX82_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOF52966.1"
                     /translation="MNFEAIRAEQTGAVLQITLNRPEARNAMSLKMVGELRQALAVAE
                     ASAGKDGAVRVVVVRGAGGHFCAGGDLKDMAGARMRAMQNTDAAQDPIAEVNAEFGQL
                     CVDYSRTPLAVVTVLEGTVMGGGFGLACVSDVAIASDTANFRLPETSLGVVPAQIGPF
                     LVERLGYSQARRLAVTGGRLDAHAALGLGLVHAVTSTDGLQAELDRVLTDILACAPGA
                     LAATKALMARARFAAPQDMVAEAAAIFSRAVQGPEGLEGTTAFIQKRKPNWALQ"
     gene            complement(1755..>2233)
                     /locus_tag="PKX82_11720"
     CDS             complement(1755..>2233)
                     /locus_tag="PKX82_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF52967.1"
                     /translation="QEGMGFMLQMLQFQEERLYGAAGALRSLDRLIDQTIDYTRQRKT
                     FGQPILNNQVVHFRLAELRTEVEALRALTYSAVESYVSGKDVTRLASMAKLKAGRLSR
                     EVADSCLQYWGGMGFTADNPISQSFRDGRLISIGGGADEIMLGIICKYEGTLPGKA"
BASE COUNT          406 a          742 c          691 g          394 t
ORIGIN      
        1 catagccggt gatgcattcg gtgaccgggt gctcgacctg cagacgggtg ttcatttcca
       61 ggaaatagtg gttgagcttg tcatccacaa tgaattccac ggtaccggcg ccgcggtaac
      121 ccacggccag cgccgcagcc actgcgtcct tgcccatggc tgcgcgcatg gcgggtgaca
      181 ccaccgggga cggcgcttct tcaatgactt tctggcgacg gcgctgggcc gtgcaatcgc
      241 gttcgcccag gtacacggcg ttgccgtgcg catcggcaaa cacctggatt tcgatgtggc
      301 ggccgttgtc gatcaggcgc tccaacatca gcgtgccgtc gccaaaggcg ctggtcgctt
      361 cgcggcgggc accttcaatg ccctgctgca attcagcaaa atggcgcacc agccgcatgc
      421 cacggccacc gccaccggcc accgctttga ccagcagcgg gaagcccagc ttctcggctt
      481 cggcaatcag cactgcatcg ctctggtccg cacccaggta gcccggtgca cacggcacac
      541 cggcgtcgag catgcggcgc ttggccagtg ctttgtcgcc catggcttca atcgcgctgg
      601 gcggtggccc aatgaagacc acattggcgt ccacacaggc ctgcgcaaag gccgcatttt
      661 cactcaaaaa accgtagcct gggtgcagcg cgtccgcgcc ggttttgcgc gcggcatcca
      721 gcagcgcgtc gaacttcagg taggactcgg cggcaggcga gccgccaatg tacacggcct
      781 cgtcggccag cagcacgtgc ggtgcgtcgc ggtcggcatc gctgtaaaca gctacggttt
      841 ggtagcccag gtttcttgcg gtgcgaatca cccggcaggc gatctcacca cggttggcaa
      901 tcaggatttt ggagaatgtc attgcaacgc ccagtttggt ttgcgttttt ggatgaatgc
      961 ggtcgtgcct tccagacctt cagggccctg aacggcacgc gaaaaaatgg ctgccgcttc
     1021 cgccaccatg tcctggggcg cggcaaaccg ggcccgggcc atcagtgcct tggtggcagc
     1081 cagggcgcca ggagcgcagg ccaggatgtc ggtcaacacc cggtcgagtt ccgcctgcag
     1141 gccgtcggtc gatgtcacgg cgtgcaccag gcccaagccc agcgcagcgt gcgcgtcaag
     1201 ccggccgcca gtcactgcca ggcgacgcgc ctgggaatac cccagacgct ccaccaggaa
     1261 ggggccgatc tgcgctggca ccacacccag cgaggtctcc ggcagacgga agttggcagt
     1321 gtcactggct atggccacat cggacacaca ggccaggcca aagccgccgc ccatgaccgt
     1381 accttccagc acggtcacca cggccagcgg tgtgcgggag tagtccacgc acaactggcc
     1441 aaactcggcg ttgacctcgg caatcgggtc ctgcgcagcg tcggtgttct gcatggcacg
     1501 catacgagcc ccggccatgt ccttgaggtc accaccggca cagaaatggc cgcctgcacc
     1561 gcgcaccacc accacgcgca cggcgccatc cttgccagcc gaggcttccg ccacagccag
     1621 tgcctggcgc aactcgccca ccatcttgag cgacatggca ttgcgcgcct cgggacggtt
     1681 cagcgtgatt tgaagcacag cccccgtctg ctctgcgcgg attgcttcga aattcatagc
     1741 actgcaatcc acggtcaagc cttgccgggc aaggtacctt cgtacttgca gatgatgccc
     1801 agcatgattt cgtcggcgcc accgccgatg gagatcaggc ggccgtcacg gaacgactgg
     1861 gaaatggggt tgtcggccgt gaagcccata ccaccccagt actgcaggca gctgtcggcc
     1921 acttcgcgcg acaggcgtcc ggccttgagc ttggccatgg aagccaggcg ggtcacatcc
     1981 ttgccgctca cataggattc cactgcgctg taggtcagcg cccgcagggc ttccacttcg
     2041 gtgcgcagtt cggccaggcg gaaatgaacc acctggttgt tcaggatggg ctggccaaag
     2101 gtcttgcgtt ggcgggtgta gtcaatggtc tggtcgatca ggcggtccag cgaacgcagg
     2161 gcaccggcgg caccgtacag gcgctcttcc tggaactgca gcatctgcag catgaagccc
     2221 atgccttcct ggc
//
LOCUS       DAOSJM010000390         2233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_42001_length_2233_cov_4.392562, whole genome shotgun sequence.
ACCESSION   DAOSJM010000390 DAOSJM010000000
VERSION     DAOSJM010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42001_length_2233_cov_4.392562"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PKX82_11725"
     CDS             <1..397
                     /locus_tag="PKX82_11725"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866075.1"
                     /note="catalyzes the formation of arginosuccinate from
                     citrulline and aspartate in arginine biosynthesis; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOF52968.1"
                     /translation="HNEDTIEQYRDNGRKLGRLLYQGRWFDSQAIMLRETAQRWVAKA
                     VTGAVTMELRRGNDYSLLNTESPNLTYAPERLSMEKVEDAPFSPLDRIGQLTMRNLDI
                     TDTRAKLQVYSNAGLLELGKGDDFLKLGK"
     gene            complement(407..>2233)
                     /locus_tag="PKX82_11730"
     CDS             complement(407..>2233)
                     /locus_tag="PKX82_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOF52969.1"
                     /translation="KTSYEVGYAQNLRDALPHATFVAFTGTPVSGADRDTRAVFGDYI
                     HVYDMQQAKEDGATVAIYYESRLAKLRLKDEDLLSIDDEVDELAEDEEENAQAQLKSR
                     WAALEKVVGAKPRIESVAADLVAHFEERNKTQSGKAMVVAMSRDICVLLYDAITALRP
                     DWHDTDPEKGAIKVIMTGSASDRALLRPHIYSAQTKKRLEKRFKDPKDPLRLVIVRDM
                     WLTGFDAPCVHTLYVDKPMKGHNLMQAIARVNRVFKGKQGGLVVDYIGIGNELKAAMK
                     EYTASKGRGRPTVDAHEAYSVLAEKMDVLRAMLHGFDYSGFLTGGHKTLAGAANHVLG
                     IKDDKKRFADVALAMSKAFTLCCTLDEAKAVREEVAFLQGVKVILTKRDVAAQKRTDE
                     ARELAIKQIIDSAVVSESVVDIFDAVGLEKPNIGLLDDEFLAQVRNLPERNLAVELLE
                     RLLEGEIKSKFASNIVQERKFSELLTNVIARYQNRSIETAQVMEELVDMAKKFREAAG
                     RGEALGLNDDEVRFYDALATNESAVRELTDETLKKIAHELTENLRQNISVDWSERESV
                     RAKLRLMVKRILRKYKYPPDLQDAAVELVLQQAQALGESWGE"
BASE COUNT          402 a          728 c          616 g          487 t
ORIGIN      
        1 ccacaacgaa gacaccatcg agcagtaccg cgacaacggc cgcaagctcg gccgcttgct
       61 gtaccagggc cgctggttcg acagccaggc catcatgctg cgcgaaaccg cccagcgctg
      121 ggtggccaag gccgtgaccg gcgccgtgac catggaactg cgccgcggca acgactacag
      181 cctgctcaac accgaaagcc ccaacctgac ctacgcgccc gagcgcctga gcatggaaaa
      241 ggtggaagac gcgccgttca gcccgctgga ccgcatcggc caactgacca tgcgcaacct
      301 ggacatcacc gacacccgcg ccaagctgca ggtgtactcg aatgctgggc tgctggagtt
      361 gggcaagggg gatgatttct tgaagctggg taagtaagcc gctgcgctac tcgccccagc
      421 tttcacccaa ggcctgtgcc tgctgcaata ccagctccac agctgcgtct tgcagatcag
      481 gcgggtattt gtacttgcgc agaatgcgct tgaccatcag tcgcaatttg gcacgcacgc
      541 tctcgcgttc cgaccagtcg actgagatgt tttgccgcag gttttcggtg agctcgtggg
      601 caattttctt tagggtctcg tcggtcagtt cccgcaccgc agactcattg gttgccagcg
      661 cgtcgtagaa gcgcacctca tcgtcgttca ggcccagcgc ttcaccccgg ccagcggctt
      721 cacggaactt cttggccata tccaccagtt cttccatgac ctgggccgtc tcaatcgacc
      781 ggttctggta gcgggcaatc acgttggtca gcaactcgga gaatttgcgc tcctgcacga
      841 tgttgctggc gaatttgctt ttgatctctc cctccagcag ccgctccagc aactccaccg
      901 ccagattgcg ctctggcaga ttgcgcactt gtgcgaggaa ctcatcgtcc agcaggccga
      961 tattgggctt ctccaacccc acggcatcaa aaatgtccac cacgctctct gacaccactg
     1021 ccgagtcaat gatctgcttg atcgccaact ctcgggcttc gtcggtgcgt ttctgcgctg
     1081 ccacatcgcg cttggtcaaa atcactttga cgccttgtag aaacgccacc tcttcgcgca
     1141 ccgcttttgc ctcatccaac gtgcagcaca aggtaaacgc tttgctcatc gccagcgcca
     1201 catcggcaaa tcgctttttg tcgtctttga tacccagcac atggttggcc gcgcctgcca
     1261 gcgtcttgtg cccgccggtc aaaaagccgc tgtagtcaaa gccgtgcagc atcgcgcgca
     1321 gcacgtccat cttttccgcc aacacgctat aggcctcgtg ggcatccacc gtggggcgtc
     1381 cgcggccttt gctggcggtg tactccttca ttgcggcttt gagctcgttg ccaatgccga
     1441 tgtagtccac caccagccca ccctgcttgc ccttgaacac ccggttcacg cgcgcaatgg
     1501 cctgcatcag gttgtgcccc ttcatgggct tgtccacgta gagggtgtgc acacaaggcg
     1561 catcaaagcc ggtcagccac atgtcgcgca cgatcaccag gcgcagaggg tccttggggt
     1621 ctttgaagcg cttttcgagc cgcttcttgg tctgggcgct gtagatgtgg gggcgcagca
     1681 gggcgcggtc tgatgccgag cctgtcatga tcactttgat ggcacccttc tccgggtcgg
     1741 tgtcgtgcca atcgggtcgc agcgcggtga tcgcgtcata gagcagcaca caaatatcgc
     1801 ggctcatcgc caccaccatg gctttgcccg actgcgtttt gttgcgctcc tcaaaatgcg
     1861 ccaccagatc tgcggcaaca ctttcgatgc ggggcttggc ccccaccacc ttttccagcg
     1921 ctgcccagcg gctcttgagt tgggcttggg cgttctcttc ctcgtcctcg gccagctcat
     1981 cgacctcgtc gtcgatggag agcaagtctt cgtccttcag gcgcagcttg gccaggcggc
     2041 tctcgtaata gatggcgacc gtggcaccat cttccttggc ctgctgcatg tcataaacgt
     2101 ggatgtagtc accaaacacc gcgcgggtat cgcggtctgc accgctcact ggcgtgccgg
     2161 taaacgcgac gaatgtcgca tggggcaagg cgtcgcgcag gttctgcgcg tagcctactt
     2221 cgtagctagt ttt
//
LOCUS       DAOSJM010000391         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_42615_length_2209_cov_4.214485, whole genome shotgun sequence.
ACCESSION   DAOSJM010000391 DAOSJM010000000
VERSION     DAOSJM010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42615_length_2209_cov_4.214485"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PKX82_11735"
     CDS             complement(<1..632)
                     /locus_tag="PKX82_11735"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOF52970.1"
                     /translation="MARVPAVPKPQGLGMDSTAVRMNMVQKLRGQGITDAAVLAAMGA
                     IERHRFVDSGLVNQAYEDTSLPIGLGQTISKPGVVSRMAELMRNGASGKIGRVLEIGT
                     GCGYQAAVLSLLATEVYSIERLRGLHDKARDNLRHLRLPNVHLLFGDGMEGYAKGAPY
                     AAIIAAAGGEAVPQAWVEQLAVGGRLVAPVVTGTGKGSTQALLVLDKTPQG"
     gene            complement(722..1501)
                     /gene="surE"
                     /locus_tag="PKX82_11740"
     CDS             complement(722..1501)
                     /gene="surE"
                     /locus_tag="PKX82_11740"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOF52971.1"
                     /translation="MKILISNDDGYQAPGIVALYEALKDIAEVEVIAPEQNNSAKSNA
                     LTLHSPLYVHKASNGFRYVNGTPADCVHIALTGLLDYRPDLVVSGINNGANMGDDTIY
                     SGTVGAAMEGYLFGIPAIAFSQVEKDWHELESAARKARELVQGMLQQQLVGQTPWLLN
                     VNIPNRPYADIKSMKLCRLGRRHAAEKVIKQQSPRGETMYWIGAAGAVMDDADGTDFH
                     ATAQGHMAMTPLKVDLTDHEALRYWAQTASRLSALPSSAAA"
     gene            1624..>2209
                     /locus_tag="PKX82_11745"
     CDS             1624..>2209
                     /locus_tag="PKX82_11745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HOF52972.1"
                     /translation="MHAWLCENPTGIDALNWKELPTPAPKAGEVLIEIKAASLNFPDI
                     LIVQNKYQMKPALPFVPGSEYAGIVQAVGEGVTHLKVGQAVACLSGTGGFGTHTLAPA
                     ALCMPLPPGFPMVDAAAFIMIYATSHHALVDRAQLKAGETVLVLGAAGGVGTSAIQIA
                     KAMGARVIAAASTDEKCDICKKIGADATINYSTEN"
BASE COUNT          402 a          707 c          662 g          438 t
ORIGIN      
        1 ccctggggcg ttttgtccag caccagcagc gcctgggtgc tgcccttgcc tgtacccgtc
       61 accacgggcg ccaccaggcg cccgccgacc gccaattgct ccacccaggc ctgcggcacc
      121 gcttcacccc ccgcggcggc aatgatggcg gcgtagggtg cgcccttggc atagccttcc
      181 atgccatcgc caaacagcag atgcacattg ggcaagcgca ggtggcgcag gttgtcgcgt
      241 gccttgtcgt gcaggccgcg caagcgctcg atgctgtata cctcggtggc cagcaggctc
      301 agcacggcgg cctggtatcc gcacccggta ccgatctcca gcaccctgcc gatttttccc
      361 gaggcaccgt tgcgcatcag ttctgccata cgcgagacca caccgggctt ggaaatggtc
      421 tgccccagcc cgatgggcag gctggtgtct tcgtaggcct ggttgaccag tccgctgtcg
      481 acaaagcggt ggcgctcgat cgcgcccatc gccgcaagaa ctgccgcgtc agtaataccc
      541 tgccctctga gcttctgcac catgttcatg cgcacggctg tcgagtccat gcccagtccc
      601 tggggcttgg gcaccgctgg cacacgggcc atgctggcag atggcgcaac aggcgccccc
      661 tgtgcaccag tgcgtgcgcg aactgcggta tcgagttttg cgggaaatgc tggccgctgc
      721 ttcatgcggc agctgaggat ggaagggccg agagacgcga ggcggtttgc gcccagtaac
      781 gcagcgcttc gtggtccgtc agatccactt tcagtggcgt catggccatg tgtccctgtg
      841 ccgtggcgtg gaaatcggtg ccatccgcat cgtccatcac tgcgccagct gcaccaatcc
      901 agtacatggt ttcgccccgt ggactctgct gcttgatcac cttctcggcg gcgtggcgtc
      961 tacccagacg gcacagcttc atgctcttga tatcagcata aggccggttg ggaatattga
     1021 cattgagtag ccagggggtc tgccccacca gttgctgctg cagcatgccc tgcaccagtt
     1081 cgcgggcttt tcgggcggcg gactccagtt cgtgccagtc tttctccacc tgtgagaaag
     1141 caatggccgg gatgccaaaa agataacctt ccatggcagc acccaccgta ccggagtaaa
     1201 tggtgtcatc gcccatgttg gcaccgttat tgatgcccga aaccaccaga tcgggccggt
     1261 agtccagcag cccggtcagc gcaatgtgca cacagtccgc gggggtaccg ttgacatagc
     1321 ggaatccatt ggaagctttg tgtacataca gcggcgaatg cagcgtcagc gcattggact
     1381 tggcgctgtt gttctgctcg ggggcaatta cctccacttc ggcaatgtct ttgagtgctt
     1441 catacagagc cacaatgcct ggcgcctggt agccgtcgtc gttggagatg agtattttca
     1501 tggtcattgc gggtaagtgc tgattgtagg tggaggcccc tgccggtcac gcaagagaca
     1561 atggccttgg cgcggaaatg ccgcatccgc ctatgatggc cgcactattt ttggagaccc
     1621 cacatgcatg cctggctttg cgaaaacccc accggtatcg acgccctgaa ctggaaagaa
     1681 ttgcccacac cggcgccgaa ggccggtgaa gtgctcatcg aaatcaaggc cgccagcctg
     1741 aatttcccgg acatccttat cgtgcagaac aagtaccaga tgaagccggc cctgcccttt
     1801 gtgccgggtt cggaatacgc tggcatcgtg caggccgtgg gcgaaggcgt cacccacctc
     1861 aaagtgggcc aggccgtggc ctgcctgagt ggcaccggcg gctttggcac ccacacgctg
     1921 gcacccgccg cactgtgcat gccgctgccg ccaggcttcc cgatggtgga tgccgccgcc
     1981 ttcatcatga tctacgccac ctcccaccac gcgctggtgg accgcgccca gctcaaggcc
     2041 ggtgaaaccg tgctggtact gggggctgcc ggtggtgtgg gcacctcggc catccagatc
     2101 gccaaggcca tgggtgccag ggtgattgct gcggcctcta ccgatgaaaa gtgcgacatt
     2161 tgcaagaaga tcggcgccga tgccaccatc aattactcca ccgaaaacc
//
LOCUS       DAOSJM010000392         2205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_42725_length_2205_cov_3.873488, whole genome shotgun sequence.
ACCESSION   DAOSJM010000392 DAOSJM010000000
VERSION     DAOSJM010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42725_length_2205_cov_3.873488"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PKX82_11750"
     CDS             complement(<1..711)
                     /locus_tag="PKX82_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOF52973.1"
                     /translation="MQHTTHDAVLRQARLRLTEYGDCSSSGLDERLARSWRRSLAAGL
                     MPRESRQEAVHADSLALRQALACNHDLLAHSQPVMEFLLEQVRHSESMVILADKAGTL
                     VHTLGHATFLDKAQQVALTRGASWLEQHRGTNAIGTALAEVSGIEIHGGEHFFERNAF
                     LTCTAAPIVSGRGELLGILDISGDQRHGHPHTLGLVSMAARMIENRLLTASTRRQTRL
                     HLHASPEGIGSVAEGIVVL"
     gene            1013..>2205
                     /locus_tag="PKX82_11755"
     CDS             1013..>2205
                     /locus_tag="PKX82_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOF52974.1"
                     /translation="MIYAAPGAAGAKIAYKAQYNNFIGGKWVAPVKGQYFDVITPVSG
                     KVYTQAARSTAEDVELALDAAHAAADAWGKTDAATRSNILLKIADRIEANLELLAYAE
                     TVDNGKAIRETLNADIPLTVDHFRYFAGCLRAQEGGISEIDENTMAYHIQEPLGVVGQ
                     IIPWNFPILMAAWKLAPAIGAGNCVVLKPAESTPISILILAELIADLLPAGVLNIVNG
                     YGREAGMPLATSKRIAKIAFTGSTSTGRVIAQAAANNLIPATLELGGKSPNIFFADIM
                     DKDDAFLDKAIEGLVLFAFNQGEVCTCPSRALIQESIYDKFMERVLKRVAAIKHANPL
                     DTDSMMGAQASKEQLTKILSYLDLGKQEGAQVLAGGGQAHLGGDLEGGYYVQPTLFKG
                     HNKMR"
BASE COUNT          448 a          714 c          653 g          390 t
ORIGIN      
        1 cagcaccaca atgccttcgg ccaccgagcc aatgccctcg gggctggcat gcagatgcag
       61 gcgcgtctgg cgccgggtgg atgcggtcag cagccggttt tcgatcatgc gggccgccat
      121 gctaaccagg cccagcgtgt gcgggtggcc atggcgctgg tcgcccgaga tatccagaat
      181 acccagaagc tcaccccggc ccgagacgat gggcgcagca gtgcaggtca ggaacgcatt
      241 gcgctcgaag aaatgttcgc cgccatggat ttcgatccca ctgacttcgg ccagtgccgt
      301 gccaatggcg ttggtgccgc ggtgctgctc cagccaggaa gcgccacggg tcagggctac
      361 ctgctgggct ttatccagaa atgtggcatg gcccagcgtg tgcaccagcg taccggcctt
      421 gtccgccagg atcaccatgc tctcgctgtg gcgcacctgc tcaagcagaa actccatcac
      481 cggctgcgaa tgggccagca ggtcgtggtt gcaggccagc gcttggcgca acgccaggct
      541 gtccgcgtgc accgcctcct gccggctttc gcgtggcatc aggccggcgg ccagactgcg
      601 ccgccaggac cgcgccagcc gctcgtccag gccgctggaa gaacagtcgc cgtattcggt
      661 cagacgcagc cgggcctggc gtagtacggc gtcgtgggtg gtgtgttgca cagtgtctcc
      721 tgcacaggct atgggcccat gtcatgtctt tgtggatagt gatggtagcc agtcgggctg
      781 acaagcgccc aacgcaaaaa aaccctgtgg acagcatgcg acaggtgtca ctttttgtga
      841 cacctgtcgc agttcgctcc tgcgggttta cccggaagtc cgccctaccc cttagaaaaa
      901 agtcttttga aatcaaagcc atgccagcgc tgcaacaact ggcacaggac ttgaggatgg
      961 aacagcaccc gcaagggaat ggcttatcca atttctaatt tctggagaca cgatgattta
     1021 cgcagcaccc ggcgcagcag gcgccaagat tgcctacaag gcgcagtaca acaacttcat
     1081 tggcggtaaa tgggttgccc cggtcaaggg ccagtacttt gacgtcatca cccctgtgag
     1141 cggcaaggtc tacacccagg ccgcccgctc cacggccgaa gacgtggaac tggcgctgga
     1201 cgccgcccat gccgcagccg acgcctgggg caagaccgac gccgccacgc gcagcaacat
     1261 cctgctgaag attgctgacc gcatcgaagc caatctggaa ctgctggcct acgccgagac
     1321 cgtggacaac ggcaaggcca tccgcgaaac gctgaacgcc gacattccct tgacggtgga
     1381 ccacttccgc tactttgccg gttgcctgcg cgcgcaggaa ggcggcatca gcgagatcga
     1441 cgagaacacc atggcgtacc acatccagga accgttgggc gtggtcggcc agatcattcc
     1501 ctggaacttc cccatcctga tggccgcctg gaaactggcg cctgccattg gtgccggcaa
     1561 ctgcgtagtg ctcaagcctg cagagtccac ccccatctcc atcctgatcc tggccgagct
     1621 gattgccgac ctgctgcccg ctggcgtgct caacatcgtc aacggctatg gccgcgaagc
     1681 cggcatgcct ttggcaacct ccaagcgcat tgccaagatt gcgttcaccg gctccaccag
     1741 caccggccgc gtgattgcac aggccgccgc caacaacctg attcccgcca cgctggagtt
     1801 gggtggcaag agccccaaca tcttctttgc cgacatcatg gacaaggacg atgccttcct
     1861 ggacaaggcc attgaaggcc tggtgctgtt cgcgttcaac cagggcgagg tctgcacctg
     1921 ccccagccgt gcgctaattc aggaatccat ctacgacaag ttcatggaac gtgtcttgaa
     1981 acgtgtggct gccatcaagc acgccaaccc gctggacacc gacagcatga tgggcgcaca
     2041 ggcttccaaa gagcaactca ccaagatcct gtcctacctc gatctgggca agcaggaagg
     2101 cgcgcaagtg ctggccggcg gcggtcaggc ccatctgggc ggcgacctgg aaggcggcta
     2161 ctacgtgcag cccacgctgt tcaagggcca caacaagatg cgcat
//
LOCUS       DAOSJM010000393         2201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_42842_length_2201_cov_4.115564, whole genome shotgun sequence.
ACCESSION   DAOSJM010000393 DAOSJM010000000
VERSION     DAOSJM010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42842_length_2201_cov_4.115564"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PKX82_11760"
     CDS             <1..327
                     /locus_tag="PKX82_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF52975.1"
                     /translation="PTANRLIELHVRPRQMEGKEVIEFAVQDTGKGLAPEVMDRLYEA
                     FYSTKANGMGIGLSLCRSIVESHLGRMTAENIYNGSVVAGCRFSFWIPISEVGVPQPR
                     TPAKSN"
     gene            344..970
                     /locus_tag="PKX82_11765"
     CDS             344..970
                     /locus_tag="PKX82_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF52976.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKGYRVRCFESAESFL
                     SRYDAREVACLIVDIRMAAMTGLELQNRLIEARSPLPIVFITGHGDVPMAVDTMKKGA
                     MDFIQKPFNEEQLVGLVERMLDHAKDTFADYQLSVSRDTLLSKLTLRESQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLSANTVADLLKIALGQNAPKA"
     gene            1042..1911
                     /gene="folD"
                     /locus_tag="PKX82_11770"
     CDS             1042..1911
                     /gene="folD"
                     /locus_tag="PKX82_11770"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOF52977.1"
                     /translation="MPTSTTAQIIDGNALSRQLGTEVTQRAAALRGRGLPPGLAVVLV
                     GDNQASQVYVRNKVKACHESGLHSVLEQYPATMTEAELLARVDVLNNDPTIHGILVQL
                     PLPAHIDAQKVIEAISPAKDVDGFHVASAGALMVGQPGFWPCTPYGCMKMLESIGYDL
                     RGKHAVVIGRSNIVGKPMALMLLAKNATVTVCHSATKDLKAMTLQADVIVAAVGKRNV
                     LTADMVKPGAVVIDVGMNRNDEGKLCGDVDFAGVSQVASHITPVPGGVGPMTITMLLV
                     NTIEAAEREANKA"
     gene            1908..>2201
                     /locus_tag="PKX82_11775"
     CDS             1908..>2201
                     /locus_tag="PKX82_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HOF52978.1"
                     /translation="MNPLLRFDALPLFDQITPAHVEPAIDTLLANASAALEKVTAPDF
                     PADWSAIARTLDVATEQLGMAWGAVSHLNSVADTPELRAAYNAALPKVTEFWTR"
BASE COUNT          459 a          719 c          651 g          372 t
ORIGIN      
        1 cccacggcca accggctgat tgagctgcac gtgcgcccac gccagatgga aggcaaggaa
       61 gtgattgaat tcgccgtgca ggacaccggc aagggcctcg ctccggaggt gatggaccgc
      121 ctgtacgaag cgttctactc caccaaggct aatggcatgg gtatcggcct gtccctgtgc
      181 cgctccatcg tggagtcgca cctgggtagg atgaccgcag agaacatcta caatggctca
      241 gtggtggccg ggtgccggtt ttcgttctgg attccgattt ccgaggttgg tgttccccaa
      301 ccccgcaccc ccgccaaaag caactagaaa attggacgtc tgaatgagct tgattcccaa
      361 aaaaggtacg gtatatgtgg tcgacgatga cgaggccgta cgggactcac tccagtggct
      421 gcttgaaggc aaaggttacc gggtacgctg cttcgagtct gccgaatctt tcctgagccg
      481 ctatgacgcg cgcgaggtag cctgcctgat cgtggacatc cgcatggcgg ccatgaccgg
      541 tctggaactg cagaatcgcc tgatcgaagc ccgctcacca ctgcccatcg tgttcatcac
      601 cggccacggc gacgtgccca tggcggtgga caccatgaaa aaaggcgcca tggatttcat
      661 ccagaagccc ttcaacgaag agcaactggt gggcttggtc gagcgcatgc tggaccacgc
      721 caaggacacc tttgccgact accagctctc ggtcagccgc gacaccctgc tctccaagct
      781 gacgctgcgc gaatcgcagg tgctcgagcg catcgtggcc ggacgcctga acaagcagat
      841 tgccgacgat ctgggcatca gcatcaagac cgtcgaagcc caccgcgcca atatcatgga
      901 gaagctcagc gccaacacgg tggcggatct tctgaaaatt gcactcggac agaacgcccc
      961 caaagcctga cacacccaat tcaatagcat caaacgcctg tcccatgagg gttacgggcg
     1021 tttttcaatt caggaacccc tatgcccaca tcgaccaccg cccagatcat tgacggcaac
     1081 gccctgtccc gccagttggg caccgaagtc acccagcgcg cggcagcctt gcgcggccgg
     1141 ggcctgcccc ccggactggc tgtggtgctg gtgggtgaca accaggccag ccaggtctat
     1201 gtgcgcaaca aggtcaaggc ctgccacgaa agcggcctgc actcggtgct ggagcagtac
     1261 cccgccacca tgaccgaagc cgagctgctg gcccgtgtgg atgtgttgaa caacgacccc
     1321 accatccacg gcatcctggt acagctaccg ctgcccgccc acatcgatgc gcaaaaggtg
     1381 atcgaggcga tttctcccgc caaggacgtg gacgggttcc acgtcgccag cgccggcgcg
     1441 ctgatggtcg gacagcctgg cttctggccc tgcacgccgt acggctgcat gaagatgctg
     1501 gagtccatcg gctacgacct gcgtggcaaa cacgcggtgg tgatcggccg cagcaatatc
     1561 gtgggcaagc ccatggcact gatgctgctg gccaagaacg ccaccgtcac cgtgtgccac
     1621 agtgccacca aagacctcaa ggccatgaca ctgcaggccg atgtgatcgt ggctgccgtg
     1681 ggcaagcgca atgtgctcac cgccgacatg gtcaaacccg gcgcggtggt gatcgacgtg
     1741 ggcatgaacc gcaatgacga aggcaagctg tgcggcgacg tggattttgc gggtgtcagc
     1801 caggtggcga gccacatcac tccggtgccc ggcggcgtcg gccccatgac catcaccatg
     1861 ctgctggtca acaccattga agccgccgaa cgcgaggcca acaaagcatg aaccccttgc
     1921 tccgctttga tgcgcttccc ctgttcgacc agatcacccc ggcccatgtc gaaccggcca
     1981 tcgacacgct gctggccaac gccagcgcgg cactggagaa agtgaccgcg cccgactttc
     2041 cagcggactg gagcgccatt gcgcgcacgc tggatgtcgc caccgagcaa ctgggcatgg
     2101 cctggggcgc cgtgagccac ctcaacagcg tggccgatac gcccgaactg cgcgcagcct
     2161 acaacgcggc cctgcccaag gtgaccgagt tctggacccg c
//
LOCUS       DAOSJM010000394         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_43438_length_2179_cov_3.195386, whole genome shotgun sequence.
ACCESSION   DAOSJM010000394 DAOSJM010000000
VERSION     DAOSJM010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43438_length_2179_cov_3.195386"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PKX82_11780"
     CDS             complement(<1..381)
                     /locus_tag="PKX82_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-alkenal reductase"
                     /protein_id="HOF52979.1"
                     /translation="MKRLWLLFSQSATVLLAAYFVVATLKPQWLNQAPTTSSAVALFE
                     APAGKPGEVPAGSFRLAAQKASAAVVSISTSKAPRRNPNANDPWFRFFYGDQGSEAQS
                     GLGSGVIVSPSGYILTNNHVIEGAD"
     gene            467..775
                     /locus_tag="PKX82_11785"
     CDS             467..775
                     /locus_tag="PKX82_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF52980.1"
                     /translation="MSKRSEVIHIHADAPAKPVQGAPCNGCGVCCLAEPCPLGMVLSR
                     KRQGACVALRWSAEDHQYRCGATQGPWGFLARRWISAGTGCDSSLQALHPLNDNGGHD
                     "
     gene            768..1529
                     /locus_tag="PKX82_11790"
     CDS             768..1529
                     /locus_tag="PKX82_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOF52981.1"
                     /translation="MTDRLELLTTLDALLQPERFKDYCHNGLQVEGRTQVRKIVSGVT
                     ASRALIEAAIEAQADAIFVHHGLFWRGFEGRIAGWMRQRLGLLLQHDINLFAYHLPLD
                     AHPELGNNAQLGRVLGLEGPAGRIGEQSLCWLGGAAGGGAFENAQALASHVENKLKRS
                     VTLVDAAQGAIKKVAWCTGGAQGYFEAAIAAGADAFITGEISEPQAHYARECGVAYLA
                     CGHHASERYGAQAVACHVAVQLGLEHVFIDIDNPA"
     gene            1533..>2179
                     /locus_tag="PKX82_11795"
     CDS             1533..>2179
                     /locus_tag="PKX82_11795"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOF52982.1"
                     /translation="MTADTTTPVMAITQGDAAGIGPEIIAKCFFSHPEVMRGCFVAGD
                     VRTMRTAASLLAAPGQLRMPVAQIANPEEAKDVPPHCIPVLQVGPPMAPVAVGQVSGI
                     AGEFAGRCVQWAAEAALRGEIAALVTAPLHKEALSAAGAPYSQFPGHTEMLQAAAARH
                     LGKAVQDVPVRMMLANDELRTVLVSIHVSLRDALAAVTYDNVLQTILITHAALTAA"
BASE COUNT          377 a          642 c          743 g          417 t
ORIGIN      
        1 gtcggcacct tcaatcacat ggttgttggt caggatgtag ccgctggggc tgacgatcac
       61 accgctgccc aggccgcttt gcgcctcact cccttggtcg ccatagaaaa agcggaacca
      121 ggggtcgttg gcattggggt tcctgcgcgg tgctttgctg gtgctgatgc tcaccacggc
      181 ggcagacgcc ttctgcgccg ccagccggaa gctgcctgcg ggcacttccc ctggtttgcc
      241 ggcgggtgcc tcaaacagcg cgactgcgct ggaagtggtg ggtgcctggt tgagccattg
      301 gggcttcaag gttgccacca caaaatatgc agcgagcaac acggttgcgg attgggagaa
      361 aagcagccag agtcgtttca tgcggcaaat tgtagggcgc aaccccgcaa gtcaagccca
      421 ggctgcggat atggcaccgt ttatcgcagt acattgcggg gaaaccatgt ccaagcgcag
      481 cgaagtcatc catatccatg cggacgcgcc cgccaagccg gtacagggcg caccctgcaa
      541 cggttgtggt gtgtgctgtc tggcggagcc gtgtccgctg gggatggtgc tgtcccgcaa
      601 acgccagggt gcctgcgtgg cgctgcgctg gagtgctgag gaccaccaat accgttgcgg
      661 cgcgacacag ggcccctggg gttttctggc ccgccgatgg atttctgcgg gaaccgggtg
      721 cgacagcagc ctgcaggccc tgcatccact caacgacaat ggtggccatg actgatcgcc
      781 tggaattgct caccacgctc gatgcgttgc tgcagcccga acgcttcaag gactattgcc
      841 ataacggcct gcaggtcgaa ggccgcacgc aggtgcgcaa aattgtttct ggcgttacgg
      901 ccagccgtgc gctgatcgag gctgccatcg aagcccaggc tgatgccatt tttgtgcacc
      961 atggcctgtt ctggcgcggg tttgaaggcc gcattgccgg ctggatgcgc cagcggctgg
     1021 gtttgctgtt gcagcacgat atcaatctgt ttgcctacca tctgccgctg gacgcccatc
     1081 ccgagctcgg caacaacgcc cagctgggcc gggtactggg gctggagggc cctgcgggtc
     1141 ggatcggcga acagtcgctg tgctggctgg gtggggcggc gggcggcggc gcattcgaga
     1201 atgcccaggc gctggccagc catgtggaaa acaagctgaa acggtcggta accctcgtcg
     1261 atgctgcgca aggtgctata aaaaaagtag cgtggtgcac gggtggtgcg caaggctact
     1321 ttgaggcggc tattgccgca ggggcagatg ccttcatcac tggagaaatc tcggagcccc
     1381 aggcccacta tgcgcgcgag tgtggcgtgg cgtatctggc ctgtggccac catgccagcg
     1441 agcgctacgg tgcccaggcg gtggcgtgcc atgtcgctgt gcagctgggg ctggagcatg
     1501 tgtttattga catcgacaac ccggcctgag ccatgactgc tgacacaacg acgcctgtaa
     1561 tggccattac ccagggcgac gcagccggga tcggcccgga aatcatcgcc aaatgctttt
     1621 tcagccatcc cgaggtgatg cgcggctgtt ttgtcgccgg tgacgtgcgg acgatgcgca
     1681 ctgcggcaag tttgctggct gcgcccgggc aactccggat gccggtggcg cagattgcaa
     1741 atccggaaga agcgaaggat gtcccgcccc actgcattcc ggtgctgcag gtggggccgc
     1801 ccatggcgcc cgtggcggtg gggcaggtca gtggcatcgc cggcgagttc gcggggcgct
     1861 gcgtgcagtg ggctgccgaa gcggctttgc gtggcgagat tgctgccctg gtgaccgcgc
     1921 ccttgcacaa ggaagcgctg tcggctgctg gtgcgcccta cagccagttt cccggccaca
     1981 cggaaatgct gcaggcagct gcagccaggc atcttggcaa ggcggtgcag gacgtgccgg
     2041 tgcgcatgat gctggccaat gacgagttgc gcacggtgct ggtgagcatc catgtgtcgt
     2101 tgcgtgacgc gctggctgcg gtgacgtacg acaatgtcct gcagacgatc ctcatcaccc
     2161 atgcggccct gacggccgc
//
LOCUS       DAOSJM010000395         2177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_43485_length_2177_cov_5.631951, whole genome shotgun sequence.
ACCESSION   DAOSJM010000395 DAOSJM010000000
VERSION     DAOSJM010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43485_length_2177_cov_5.631951"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="PKX82_11800"
     CDS             complement(<1..996)
                     /locus_tag="PKX82_11800"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HOF52983.1"
                     /translation="MDRNQQLFDRARQVIPGGVNSPVRAFKAVGGTPRFVARAQGAYF
                     WDANDKRYIDFIGSWGPMILGHGHPAVVEAVQKAVLEGFSFGAPTEREVELAEEILSL
                     VPSMDMVRLVSSGTEASMSAIRLARGATGRSKIIKFEGCYHGHADALLVKAGSGLATF
                     GHPTSAGVPAEVVQHTLVLEYNNLAQIEEVFATQGGEIACVMIEPIAGNMNFVRAQVP
                     FVKRIAELCQQHGALFVFDEVMTGFRVALGGAQSVYAKLIPGFAPDISVFGKVIGGGM
                     PLAAFGTSRKIMQNLAPLGPVYQAGTLSGNPVATACGLATLKEIAKPGFYEALAAK"
     gene            complement(1004..1876)
                     /locus_tag="PKX82_11805"
     CDS             complement(1004..1876)
                     /locus_tag="PKX82_11805"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOF52984.1"
                     /translation="MDDGPAASVMVFNANDPSGAAGITADLMTIASVGAHALPVITGA
                     YARDTSEVYDHFAFDDEAVSEQARAILEDVPVQAFKVGFVGTPENLSAIAELASDYSD
                     VPLITYMPDLSWWQEDQIDLYQDACTELLLPQTTVLVGNHSTLSRWLLPDWNSERSPT
                     ARDIARAANEFGVAYTLVTGIPLPDQFIDNVLSSPTAVLCSHKFERFEASFFGAGETL
                     SAALTALIASDTELTEAVSEALSYLDRCLEGGFRPGMGNVLPDRLFWAHTEDDDEEDA
                     QESSFEISPNDTRH"
     gene            2037..>2177
                     /locus_tag="PKX82_11810"
     CDS             2037..>2177
                     /locus_tag="PKX82_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOF52985.1"
                     /translation="MTDTKTWMCLICGWIYDEATGAPDHGIAPGTLWADVPMNWTCPE
                     CGA"
BASE COUNT          399 a          618 c          716 g          444 t
ORIGIN      
        1 cttggcggcc agggcctcgt agaagccggg cttggcgatt tccttcagcg tggccaggcc
       61 gcaggcggtg gccaccgggt tgcccgacag cgtgccggcc tggtaaaccg ggcccagcgg
      121 cgccaggttc tgcatgatct tgcggctggt gccaaaagcc gccagcggca tgccgccgcc
      181 gatgaccttg ccgaacacgc tgatgtcagg cgcaaagccg gggatcagct tggcgtacac
      241 gctctgcgcg ccgcccaacg ccacgcgaaa gcccgtcatc acctcgtcga agacgaacag
      301 cgcgccgtgc tgctggcaca gctcggcaat gcgcttgacg aagggcacct gggcgcgcac
      361 gaagttcatg ttgccggcga tcggctcgat catcacgcag gcgatttcac cgccctgggt
      421 ggcgaacact tcttcgatct gggccaggtt gttgtactcc agcaccaggg tgtgctgcac
      481 cacctcggcc ggcacgccgg cgctggtggg gtggccgaag gtggccaggc ccgagccggc
      541 cttgaccagc agcgcatcgg catggccgtg gtagcagcct tcaaacttga tgatcttgct
      601 gcgcccggtg gcgccacgcg ccaggcggat agcgctcatg ctggcttcgg tgccactgct
      661 taccaggcgc accatgtcca tggaaggcac caggctcagg atttcttcgg ccagttcgac
      721 ttcgcgctct gtcggtgcgc caaacgagaa gccttcgagc acggcctttt gtacggcttc
      781 caccaccgcg gggtggccgt ggcccagaat catcgggccc caggagccga tgaaatcgat
      841 gtagcgcttg tcgttggcat cccagaaata ggcaccttgc gcgcgggcta caaaacgtgg
      901 tgtgccgcct acggccttga aggcccgtac gggggagttc acgccgccgg gaatgacttg
      961 gcgggcacgg tcgaagagtt gctggttgcg gtccataggg gtttcagtgg cgggtgtcgt
     1021 tgggagagat ttcaaaactg gattcctgtg cgtcttcctc gtcgtcgtct tcggtatgtg
     1081 cccagaacag gcggtcgggc agcacattgc ccattccggg acggaagccg ccttcgaggc
     1141 agcggtcaag ataactcagg gcttcggaga cggcttcggt cagttcggtg tcgctggcga
     1201 tcagcgcagt cagtgcagcg gagagggttt cccctgcacc aaagaagctg gcttcaaagc
     1261 gttcaaactt gtgggagcac agtactgcgg tgggcgaact cagaacgttg tcgataaatt
     1321 ggtccggcaa ggggatgccg gtgaccaggg tgtaggccac accgaactcg ttggcagcgc
     1381 gggcaatgtc acgggcagtg gggctgcgtt ccgaattcca gtctgggaga agccagcggg
     1441 acaaagtgct gtggtttcca accaacactg tggtctgggg taatagtaac tctgtgcaag
     1501 cgtcctgata caaatcgatc tggtcttcct gccaccacga gaggtcgggc atgtaggtaa
     1561 tcagcggtac atctgagtag tccgaggcca gttctgcgat ggcgctgagg ttctctggcg
     1621 taccgacaaa cccgaccttg aaagcctgta ccggcacgtc ttccagaatg gcacgtgcct
     1681 gttcggaaac agcttcgtcg tcaaaggcaa agtggtcgta cacctcggag gtatcccggg
     1741 cgtaggcccc ggtaatgacg ggcagtgcgt gggcgccgac cgatgcgatg gtcatgaggt
     1801 cggcggtgat tccggcagct ccactggggt cgttggcgtt gaagaccatg acgctggccg
     1861 ccggaccgtc gtccatggag ttttcttcaa ggtcgggaga aatggatgcc ggcagcatgg
     1921 agcctgtcct tgtgtttgcc gtcaggcaga atggggcgct tcgggctggc attcactttt
     1981 tttgatgcgt ttgcctctat acaatcgttg cattctattc gaagccccta taagctgtga
     2041 ccgatacaaa aacctggatg tgtctgattt gcggttggat ttatgacgag gccactggtg
     2101 cgccggatca tggaattgca cctggtaccc tttgggcgga tgtacccatg aactggacgt
     2161 gcccggagtg tggcgcc
//
LOCUS       DAOSJM010000396         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_43639_length_2171_cov_2.847826, whole genome shotgun sequence.
ACCESSION   DAOSJM010000396 DAOSJM010000000
VERSION     DAOSJM010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43639_length_2171_cov_2.847826"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="PKX82_11815"
     CDS             complement(<1..1464)
                     /locus_tag="PKX82_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOF52986.1"
                     /translation="MDRRHLLGLATAWAGSLWLPRSAWSQVRFNSDPFALGVASGSPT
                     HDSVVLWTRLVQPAGSLPQQDLTVRWEMAHDAQFQKMAATGKATAVAALAHSVHVEVP
                     ALAPDHWYYYRFMAGDAVSATGRTRTFPAPGVAVNRLRLGYASCQHWEHGYYGAYRHM
                     QEEQLDALMFLGDYIYEYTGSSRGVRRAQGGTCVTLDDYRARYALYKSDLHLQRMHLA
                     CPWLMTWDDHEVQNDYAGLAQGYPGGEGVAFAARRAAAYQAFYEHMPLRANVLTLALA
                     GLAQGAEMRIYSSVPFGNLATLYLLDDRQYRDPQACTKDGRAGSSTANPDTCAIWNEP
                     RRTLLGAQQEQWLDQSFAKDKAQWNVLGQQTVLGQRDFKSGPGLALSNDGWDGYSAAR
                     TRLTDSMQRTRLANPVILGGDVHSNWVGHVKADYARADSATVGVEFCGTSITSHGGSN
                     ERMASVLAENPHFVFADRERRGYGVVEFTPKQLTTTLR"
     gene            complement(1490..2143)
                     /locus_tag="PKX82_11820"
     CDS             complement(1490..2143)
                     /locus_tag="PKX82_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOF52987.1"
                     /translation="MNKLKIDFVSDVSCPWCAVGLASLQTALKNLDGVVQVNMAFQPF
                     ELNPDMVPEGEDTTEHLAKKYGSTPEQAAAVRETIRERGAAVGFTFNLDKRSRIYNTF
                     DAHRLLHWAHLEDSSGVKQLALKQGLLKAYFTDGENPGSHSVLLRVAGEVGLDVARAQ
                     VILDSDTFAAEVREQEQFYQGLGIHSVPAVIINDRHLISGGQPPEVFEQALRQIAVA"
BASE COUNT          372 a          726 c          693 g          380 t
ORIGIN      
        1 ccgcagcgtg gtggtgagct gtttgggcgt gaactccacc acaccatagc cccgccgctc
       61 ccggtcggca aacacaaagt gcgggttttc cgccagcaca ctggccatgc gctcattgct
      121 gccgccgtgc gaggtgatgc tggtgccaca gaactccaca cccacggtgg cactgtctgc
      181 gcgcgcgtag tcggctttca catgccccac ccagttggaa tgcacatcac cgcccagaat
      241 caccgggttg gccagccggg tgcgctgcat ggagtcggtc aggcgggtac gtgcggcaga
      301 gtagccgtcc cagccatcgt tggacagcgc caggccgggt ccggacttga agtcgcgctg
      361 gcccaacacc gtttgctggc ccagcacatt ccactgtgcc ttgtctttgg caaacgactg
      421 gtcgagccac tgttcctgct gcgcacccag tagcgtgcga cgcggctcgt tccagatggc
      481 gcaggtgtcg gggttggctg tgctggagcc cgcccggcca tccttggtgc aggcctgcgg
      541 gtcgcggtac tgccggtcgt ccagcaggta cagcgtggcc aggttgccaa agggcacgct
      601 gctgtagata cgcatctcgg ccccctgcgc caagccggcc agggccaggg tcagcacatt
      661 ggcacgcaac ggcatgtgct cgtagaacgc ctggtaggcc gccgcacgcc gcgccgcaaa
      721 ggccacgccc tcgccgcccg gatagccctg cgccagaccc gcatagtcgt tctgcacttc
      781 gtggtcgtcc caggtcatca gccacggaca ggccaggtgc atgcgctgca ggtgcaagtc
      841 gctcttgtac agggcgtagc gggcgcggta gtcgtccagc gtgacgcaag tgcctccctg
      901 ggcccggcgc accccacggc tgctgccggt gtactcgtag atgtagtcac ccaggaacat
      961 gagcgcatcg agctgctcct cctgcatgtg ccggtaggcg ccgtagtagc cgtgctccca
     1021 gtgctggcac gaggcatagc caagccgcag gcggttgacc gcgacacctg gagcggggaa
     1081 cgtccgggtg cgcccggtag cgctgaccgc atcgccggcc atgaagcggt agtagtacca
     1141 gtggtccggt gccagtgcgg gcacctccac atgcaccgaa tgcgccagcg cggccactgc
     1201 ggtggccttg cccgttgcgg ccatcttctg gaactgggcg tcgtgcgcca tctcccagcg
     1261 cacggtcagg tcttgctgcg gcaagctacc cgcgggctgc accagccggg tccacagcac
     1321 cacgctgtcg tgcgtgggtg aaccactggc aacgcccagg gcaaacggat cgctgttaaa
     1381 acgtacctgg ctccatgcac tgcgcggcag ccatagcgag cccgcccatg ccgtggccag
     1441 gcccagcagg tgtcggcggt ccatcacggc atgcgtcccg ctggcagggt caggcgacag
     1501 cgatctggcg cagcgcctgc tcaaacactt cggggggctg gccgccggag atcagatggc
     1561 ggtcattgat gatcaccgca ggcaccgagt ggatgcccag gccctggtaa aactgctcct
     1621 gttcgcgcac ctcggccgcg aacgtgtcgc tgtccagaat gacttgggct cgcgctacat
     1681 ccagaccaac ttcgccggcc acgcgcaaca gcacgctgtg cgaacccgga ttctccccat
     1741 cggtgaaata ggccttgagc aacccctgct tcagcgccag ctgcttgacg ccgctggagt
     1801 cttccagatg tgcccagtgc agcaggcggt gcgcatcgaa ggtgttatag atgcgcgaac
     1861 gtttgtccag gttgaaggta aaacccaccg cagcgccccg ttcgcggatg gtctcgcgca
     1921 cggcagcggc ctgctccggg gtggagccgt atttcttggc caggtgctcg gtggtgtctt
     1981 cgccctcggg caccatgtcg gggttgagct caaacggctg gaaggccatg ttcacctgca
     2041 ccacgccatc gaggtttttc agtgcagtct gcaacgacgc caggcccacg gcgcaccacg
     2101 ggcaggacac atccgagacg aaatcgattt tgagcttgtt catccccgca ctttaaagca
     2161 caaagggcaa c
//
LOCUS       DAOSJM010000397         2162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_43873_length_2162_cov_6.597532, whole genome shotgun sequence.
ACCESSION   DAOSJM010000397 DAOSJM010000000
VERSION     DAOSJM010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43873_length_2162_cov_6.597532"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PKX82_11825"
     CDS             complement(<1..971)
                     /locus_tag="PKX82_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOF52988.1"
                     /translation="MPATARPTHSHSHAGGQAGTKPKLYNPRHPERTLLYQTIAEHFE
                     TWHELASAGQFDGQGDHHTPKPYVRQAFRKYLECGIFAHGFARAWCDDCGHDYFVAYS
                     CKGRGVCPSCNTRRMAETAAHLTDHVFPRLPVRQWVLSVPKRLRYFMQRDGAVLGMVL
                     RIFLRVIAQSLSANCPSAADVNKAAQHIGAVAFIHRFGSSLNGHVHFHVCAVDGVFEE
                     VPGIVFHPARAIDETAVAQVQATLRKRILRAFVGRGLLESCDAKEMLGYQHSGFSVDA
                     GVCIEAHDRAALERLLRYCARPPFAMDRLRKEGAALVYRCAKQHSEPT"
     gene            complement(1135..1428)
                     /locus_tag="PKX82_11830"
     CDS             complement(1135..1428)
                     /locus_tag="PKX82_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOF52989.1"
                     /translation="MLRASKDREQVGSSSVDYLMYSGFLMMAYFWALQASKATELLAS
                     GKGKESNDFYTAKIQTAEFYFARLLPRADSHYSAALAPTSAVMQMPQELFQMA"
     gene            1437..2120
                     /locus_tag="PKX82_11835"
     CDS             1437..2120
                     /locus_tag="PKX82_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOF52990.1"
                     /translation="MEFSVTDTGLGIAPDQQALLFQPFSQADSSTTRRFGGSGLGLSI
                     VRSLAHLMHGDVGVSSTLGQGSRFWFRIRVTCSSERLEQHSTAVAPTSAGTSADRQVG
                     GKVLVAEDNLVNGMVIEALLTQLGLEVHLVGDGQQAVDLVTSGDIPDVILMDIQMPVM
                     DGYAATEQIRRWEEIHGRSRIPIIALTADAFEEDRQHCFAVGMDDFLTKPVAMDALRT
                     ALSQWVTPA"
BASE COUNT          435 a          639 c          669 g          419 t
ORIGIN      
        1 gtgggctcgc tgtgctgttt ggcgcagcgg tacaccaggg ctgcgccctc tttgcgaagg
       61 cggtccatgg caaagggcgg gcgggcgcaa tacctcagca gccgctccag tgctgcgcgg
      121 tcgtgcgcct caatacacac accggcatcc accgaaaacc cgctgtgctg gtagcccagc
      181 atctctttgg cgtcacaact ctccagcaag ccccggccca cgaaggcgcg caggatgcgt
      241 ttgcgcaagg tagcctgcac ctgagccaca gcggtctcgt cgatagcacg ggccgggtga
      301 aagacgatgc ccggcacttc ctcaaacacc ccatcgaccg cgcacacatg gaagtgcaca
      361 tgcccgttca ggctggagcc gaaccggtgg ataaaggcca cagcgccaat gtgctgggct
      421 gccttgttca cgtccgctgc actggggcag ttggcagata ggctttgcgc aatgacccgc
      481 aagaaaatgc gcagcaccat gcccagcact gccccgtcac gctgcatgaa gtagcgcagc
      541 cgctttggta ccgacagcac ccactggcgc accggcagac ggggaaagac gtggtccgtc
      601 agatgcgccg ctgtctccgc catgcgccgg gtgttgcacg aggggcagac accccggccc
      661 ttgcaggaat aggccacaaa gtagtcgtgc ccgcagtcat cacaccaggc ccgggcaaaa
      721 ccgtgggcga agataccgca ctccagatac ttgcgaaacg cctgacggac atagggcttg
      781 ggcgtgtggt ggtcgccctg gccgtcgaac tggccggcac tggcaagttc atgccaagtc
      841 tcgaagtgct cggcgatggt ttggtacagc agggtccgct cggggtggcg cgggttgtag
      901 agctttggct ttgtacccgc ttgcccgcca gcgtgtgaat gcgagtgcgt tgggcgtgca
      961 gtggcgggca tggggcagtg ccagcgtggg ctgggtgcct gatgatactg gttttacatc
     1021 cagcaatata cacttcactg ctatcagcgg gcagccacat cactcctgaa ttgatagctg
     1081 gttgcgcata ttccacgagg gccggaggcc aaatacacta agaaaccctc agtctcaagc
     1141 catctgaaac agctcctgcg gcatctgcat caccgcgcta gtcggcgcca gcgcagcgct
     1201 gtagtggctg tccgcacggg gcagtagacg cgcaaagtag aactctgctg tctggatctt
     1261 ggcggtgtag aagtcgttgg actccttgcc cttgccactc gctaatagtt cggtggcctt
     1321 ggacgcctgc agcgcccaga agtaggccat catcaaaaag ccggagtaca tcaggtagtc
     1381 cacgctgctg gatcccacct gctcgcggtc tttggaggcg cgcagcatga gcagatctgg
     1441 agttttcggt aacagacact ggactcggca ttgcgcccga tcagcaggcg ctgttgttcc
     1501 agcctttttc ccaagccgac agttccacca cgcgacgctt tggcggctcc ggccttggcc
     1561 tgtcgattgt gcgcagtctg gcgcacttga tgcacggtga cgtgggtgtc agcagcacac
     1621 tcggacaggg ttcgcgcttc tggtttcgca ttcgggtcac ctgctcatca gagcgactgg
     1681 agcagcattc aacggcagtt gcgcctacga gtgcagggac atcagcggac cgccaggttg
     1741 gcggtaaggt gctggtggca gaagacaacc tggtcaacgg catggtgatc gaggcactgc
     1801 tgacccagct gggacttgaa gttcatctgg tgggtgacgg gcaacaagcc gttgatctgg
     1861 tcaccagcgg tgacattccg gatgtcattc tgatggacat ccagatgcca gtgatggacg
     1921 gctatgcggc gaccgaacaa atccgcaggt gggaagaaat tcatggccgg tcacgaattc
     1981 cgatcatcgc attgactgcg gacgcctttg aggaagaccg tcaacattgc tttgctgtcg
     2041 gtatggatga ttttctgacc aagccagtgg caatggacgc cttgcgcacc gcgttgagcc
     2101 agtgggtcac cccagcttaa acacgcgccc acggataagc atttcaaccg catgaggcca
     2161 ag
//
LOCUS       DAOSJM010000398         2146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_44342_length_2146_cov_3.244381, whole genome shotgun sequence.
ACCESSION   DAOSJM010000398 DAOSJM010000000
VERSION     DAOSJM010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44342_length_2146_cov_3.244381"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="PKX82_11840"
     CDS             complement(<1..848)
                     /locus_tag="PKX82_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOF52991.1"
                     /translation="MTDQPILDFAASAPEGDDQLNLATYAQRAYLEYALSVVKGRALP
                     DVCDGLKPVQRRILYSMSRMGLGFGGPNGNTGAKPVKSARVVGDVLGRFHPHGDQSAY
                     DALVRMAQDFSQRYPLVDGQGNFGSRDGDGAAAMRYTEARLSRITSLLLDEIDQGTVD
                     FQPNYDGSTEEPKQLPARLPFSLLNGASGIAVGLATEIPSHNLREIADACVALIKSPT
                     LSDDDLFALVPGPDFPGGGQIISPAGDIADAYRSGRGSLKVRARWKIEDLARGQWQLV
                     VTELPPG"
     gene            complement(854..1657)
                     /locus_tag="PKX82_11845"
     CDS             complement(854..1657)
                     /locus_tag="PKX82_11845"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOF52992.1"
                     /translation="MATFHAKADVWVHVDAQGEAHFAPQRVDDRYELFYRGPAPAQSS
                     DAAAEDAPARPPELKDLRPKLAAFFESSPRYRQLQPLIHEAARIYRIDYELLQGVIAV
                     ESAFDPEIVSSKGAIGLMQVLPDTARRFGVDSDRWSSVESKLKNPRMNLQLGARYLRF
                     LIDLFPGRLDLALASYNAGEGAVQKAGNAIPNYRETQDYVAMVTQLYTVLKPPPAPVA
                     PKSARTEPYGPGFVLTAPREPYALVQSGRGNMVPPIRIGAPSDSSIAVD"
     gene            complement(1729..>2146)
                     /locus_tag="PKX82_11850"
     CDS             complement(1729..>2146)
                     /locus_tag="PKX82_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOF52993.1"
                     /translation="LLTLFFRHFPKLIETGHVFVARPPLFRVDAPARGKKPASKAYAL
                     DEGELTAILDKLRKEGVREGAWAISRFKGLGEMNAEQLWDTTLNPDTRRLLPVELGAI
                     DFGATEALITKLMGKGEAAARRELMELHGDSVEIDV"
BASE COUNT          415 a          670 c          685 g          376 t
ORIGIN      
        1 ccaggtggca actcagtcac caccagttgc cattgaccac gcgccaggtc ctcaatcttc
       61 cagcgtgccc gcactttgag cgaaccgcga ccgctgcggt aggcatcggc aatgtcgcca
      121 gccgggctga tgatctggcc accaccggga aagtcggggc cgggtaccag cgcaaacaga
      181 tcgtcgtccg aaagtgtggg cgacttgatg agcgccacgc aggcatcggc aatttcgcgc
      241 aggttgtggc tggggatttc ggtggccagg cccacggcga taccgctggc cccgttgagc
      301 agcgagaacg gcaggcgcgc agggagctgc ttgggctctt cggtagagcc atcgtagttg
      361 ggctggaagt ccacggtgcc ctggtcgatc tcgtcgagca gcaggctggt aattctggac
      421 aggcgtgctt cggtatagcg catggcggcc gcgccatccc cgtctctgga gccgaagttt
      481 ccctggccgt ccaccaatgg atagcgctgc gaaaaatcct gcgccatgcg caccagcgcg
      541 tcgtaggccg actggtcgcc gtgcgggtgg aaacggccca gtacatcgcc caccacgcgg
      601 gcgctcttga ccggcttggc accggtgttg ccattggggc caccaaaacc cagccccatg
      661 cgggacatgg aatacagaat gcgccgctgc accggcttga ggccatcaca gacatcgggc
      721 aaggcgcggc ccttgaccac gctcagcgca tattccaggt aggcccgctg ggcgtaggtg
      781 gccaggttga gctggtcatc gccctcgggc gcagacgcag caaagtcaag aatgggttgg
      841 tcggtcatga aaactagtca actgcaatag aagaatcaga aggagcgccg atgcggatgg
      901 gcggcaccat gttgccgcgg cccgactgca ccagtgcgta gggttcgcgc ggcgcggtca
      961 gcacaaagcc ggggccatac ggctcggtgc gtgcggactt gggagccacc ggcgccggtg
     1021 gaggtttgag cacggtgtag agctgcgtga ccatggccac gtaatcctgc gtttcacggt
     1081 aattggggat ggcgttgccg gccttctgca ccgcaccctc accggcgttg taggacgcca
     1141 atgccagatc gagccggccg gggaacagat cgatcaggaa ccgcaggtag cgcgcgccca
     1201 gctgcaaatt catgcgcggg ttcttcagct tggattccac cgaagaccag cggtcgctgt
     1261 ccacgccaaa acgccgcgcg gtatcgggca gcacctgcat gaggccaatg gcgcctttgc
     1321 tggacacgat ctcgggatca aaagccgact ccaccgcgat cacaccctgc agcagttcgt
     1381 agtcgatgcg gtagatgcgc gcggcttcgt gaatcagcgg ctgcaactgg cggtagcgtg
     1441 gcgacgactc aaaaaacgcc gccagcttgg ggcgcaggtc cttgagttcc ggtggacggg
     1501 cgggtgcatc ctccgccgcc gcgtcgctgc tctgtgcagg cgcgggcccg cggtaaaaca
     1561 gctcataccg gtcgtccacg cgttggggtg cgaaatgggc ttcaccctgg gcgtccacat
     1621 gcacccagac atcggctttg gcatgaaatg tggccatacc ccccgccaat agtgcgcagg
     1681 tagctatgct ttttatagca ttgagcgaca aaatacgcca ggcagacatc agacgtcaat
     1741 ctccaccgag tcgccatgca actccatcaa ctcccgccgg gcagcggctt cacctttgcc
     1801 catgagcttg gtgatcagcg cctcggtggc gccaaagtcg atagcaccca gttccaccgg
     1861 caacagacgg cgggtgtcag gattcagcgt ggtgtcccac aactgctcgg cattcatctc
     1921 gcccaggcct ttgaagcggc tgatggccca agcgccttca cgcacaccct ctttgcgcag
     1981 cttgtccaag atggcggtga gctcgccttc gtccagcgca taggccttgc tggccggctt
     2041 tttgccacgt gcaggcgcat cgacgcggaa cagcggcgga cgggccacga acacatgccc
     2101 ggtctcgatc agcttgggga aatggcggaa aaacagcgtc agcagc
//
LOCUS       DAOSJM010000399         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_44491_length_2140_cov_4.562110, whole genome shotgun sequence.
ACCESSION   DAOSJM010000399 DAOSJM010000000
VERSION     DAOSJM010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44491_length_2140_cov_4.562110"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /gene="prfB"
                     /locus_tag="PKX82_11855"
     CDS             <1..678
                     /gene="prfB"
                     /locus_tag="PKX82_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOF52994.1"
                     /translation="ACDWASMLLRQYLKYAERKGFKTTIEDETAGDTAGIKGATIKIE
                     GEYAFGLLRTETGVHRLVRKSPFDSSGGRHTSFASVFVYPEIDDSIEIDINPSDVRTD
                     TFRASGAGGQHINKTDSAVRLTHIPTGIVVQCQDGRSQHSNRDVAWKRLRSRLYDHEM
                     RKRQAEQQKLEDSKTDVGWGHQIRSYVLDNSRIKDLRTNVEVSATQKVLDGDLDVFIE
                     ASLKQGV"
     gene            678..1376
                     /locus_tag="PKX82_11860"
     CDS             678..1376
                     /locus_tag="PKX82_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOF52995.1"
                     /translation="MTATLRMQPVKALIFDMDGTIVDSMPHHTVTWLDFAKKHGLSLD
                     VDDMMRRTTGRTGVECVRLLMDEPDMPEALAWELVHEKEQLYRDLFGPIFKEVTGFGA
                     FYKAVRARGLKVGLGTAGDIHNVEFVLNHLHLVPPPDAIARGDEGLTGKPTPAIFLAA
                     AQRIGMAPEHCIVFEDAPFGIEAARRAGMRAVAICTGHSAEELAGPHVVASARDYNEL
                     FESNFLESLHVATA"
     gene            1360..>2140
                     /gene="pepN"
                     /locus_tag="PKX82_11865"
     CDS             1360..>2140
                     /gene="pepN"
                     /locus_tag="PKX82_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOF52996.1"
                     /translation="MLQLRDDNGPGMVIHRAAYTAPAYWIDTVDLSFDLDPAKTRVLN
                     KMTLRRNPDVAAQPLRLDGEELNLARVLVNGQGTSFKMDGNQLVLENLPEGTEPFALE
                     IFTTCAPVKNTKLMGLYVSNESFFTQCEAEGFRRITYFLDRPDVMAMYTVTLRADKAK
                     YPVLLSNGNLVDSGALEEGRHFAKWVDPHKKPSYLFALVAGQLVAREQRITSRAGKDH
                     LLQVYVRPGDLDKTEHAMNSLMASVAWDEARFGLPLDLERFM"
BASE COUNT          429 a          680 c          658 g          373 t
ORIGIN      
        1 gcctgcgact gggccagcat gctgctgcgc cagtacctca aatacgccga gcgcaagggc
       61 ttcaagacca cgattgaaga cgaaaccgcg ggcgacaccg ccggcatcaa gggtgccacc
      121 atcaagatcg aaggcgaata cgcctttggc ctgctgcgca ccgagaccgg tgtacaccgc
      181 ctggtgcgca agagtccgtt cgattcgtcc ggcggccgtc acacctcgtt cgcgtcagtg
      241 tttgtctacc ccgagatcga tgactcgatc gagatcgaca tcaaccccag cgatgtgcgc
      301 accgacacct tccgcgcatc gggcgccggc ggccagcaca tcaacaagac cgactcggcg
      361 gtgcgcctga cgcacatccc caccggcatc gtggtgcagt gccaggacgg acgcagccag
      421 cacagcaacc gcgacgtcgc ctggaagcgt ctgcgttcgc gcctgtacga ccacgagatg
      481 cgcaagcgcc aggccgagca gcagaagctg gaagactcca agaccgatgt gggctggggc
      541 caccagattc gctcttacgt gctggacaac agccgcatca aggacctgcg caccaatgtc
      601 gaagtctcgg ccacccagaa ggtgctggac ggcgacctcg atgtgtttat cgaagcttcc
      661 ctcaagcaag gggtgtgatg actgccactc tgcgcatgca gccggtcaag gcattgattt
      721 tcgatatgga tggcaccatc gtcgattcca tgccgcacca cacggtgacc tggctggact
      781 ttgccaaaaa gcatggcctg tcgttggacg tcgatgacat gatgcgtcgc accaccggcc
      841 gtaccggcgt ggagtgtgtg cgtctgttga tggacgagcc cgatatgccc gaagcattgg
      901 cctgggaact ggtgcatgaa aaagagcagc tgtaccgcga cctgtttggc cccatcttca
      961 aggaagtgac cggctttggc gctttttaca aagcggttcg cgcacgcggg ctcaaggtcg
     1021 gtctgggcac tgcgggggac atccacaacg tggagtttgt gttgaaccac ctgcatctgg
     1081 tcccgccgcc cgacgcgatt gcgcgtggcg acgaaggcct gaccggcaag cccacgcccg
     1141 ccatttttct ggcagcggcc cagcgcatcg gcatggcacc ggaacattgc atcgtgtttg
     1201 aagatgcccc gtttggcatc gaggccgcgc gccgcgccgg catgcgtgcg gtggctatct
     1261 gtaccggcca cagtgccgag gaactggcgg gcccgcatgt ggtggccagc gcccgcgact
     1321 acaacgaatt gtttgaatct aattttctgg agagtctgca tgttgcaact gcgtgatgac
     1381 aatggcccgg gcatggtgat ccaccgtgcg gcctacacag ccccggcgta ctggatagac
     1441 accgtcgatc tgagcttcga cctggacccc gccaagaccc gcgtgctcaa caagatgacg
     1501 ctgcgccgca accccgacgt ggcggcccag cccctgcgcc tggacggcga ggagctgaac
     1561 ctggcgcgcg tgctggtcaa cggccagggc accagtttca agatggacgg caaccaactg
     1621 gtgctggaaa atctgcccga gggcaccgag cctttcgcgc tggaaatctt cacgacctgc
     1681 gcgccggtca agaacaccaa gctcatgggc ctgtacgtga gcaacgaatc gttcttcacg
     1741 cagtgcgagg ccgagggttt ccgccgcatc acctacttcc tggatcgccc ggatgtgatg
     1801 gccatgtaca cggtgaccct gcgcgctgac aaggccaagt acccggtgtt actgtccaac
     1861 ggcaatctgg tggacagcgg cgccctggaa gaaggccgcc acttcgccaa gtgggtggac
     1921 ccgcacaaga agcccagcta cctgttcgcc ctggtggccg gccagctggt agcgcgcgag
     1981 cagcgcatca ccagccgcgc gggcaaagac cacctgctgc aggtgtatgt gcgccccggc
     2041 gatctggaca agaccgaaca cgcgatgaat tcactcatgg ccagtgtggc ctgggacgaa
     2101 gcgcgctttg gcctgccgct ggacctggaa cgcttcatga
//
LOCUS       DAOSJM010000400         2138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_44553_length_2138_cov_3.517043, whole genome shotgun sequence.
ACCESSION   DAOSJM010000400 DAOSJM010000000
VERSION     DAOSJM010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2138
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44553_length_2138_cov_3.517043"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PKX82_11870"
     CDS             complement(<1..664)
                     /locus_tag="PKX82_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOF52997.1"
                     /translation="MLALNHWPLSNRRDLVGVFTDIDDTLTTHGAITPDALQALADLK
                     AAGLTVIAITGRPIGWCEPFMAGTTGPRWPVDAMVAENGAVAYVLDKNGLQPPRNMRS
                     ALSKLYQQDAATREANSLRMQKVAARVMFEVPGVFLSRDSVGRETDLAFDYNEFTRHT
                     PETVQKVRDALERAGMQTTTSSIHIHGCFGDFNKWQGAQWIVRELFRRDLAQELDRWA
                     FVG"
     gene            744..1550
                     /locus_tag="PKX82_11875"
     CDS             744..1550
                     /locus_tag="PKX82_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF52998.1"
                     /translation="MFRWRLWWVGWGLWLCAAGAVSAQQVAQVVDIPTRPGVTQRFLY
                     LAPAAPRAAVILIAGGHGGLQMSTAGDVRWGADNFVVRTRELFARAGMAVAVVDAPSD
                     RQTLPHLNGFRQTPEHAKDLKAVMQWLRAKHTVPVWLVGTSMGTLSAAYVGMTLPARD
                     GGPDGVVLTSSVLVAAGAKGLRPVMEMPVQQITMPVLVVHHTQDGCKYCPYDQAVALM
                     DKLSASPAKALISISGGQDKGDPCHEWAYHGFNGMEAETVTRIADWITAH"
     gene            complement(1578..>2138)
                     /locus_tag="PKX82_11880"
     CDS             complement(1578..>2138)
                     /locus_tag="PKX82_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOF52999.1"
                     /translation="EAVEEAYAGDIVGFTTHGGVQLGDTITDGSVNLMYTGLPFFAPE
                     MFMTVVLKNPLRTKQLQQGLAQLGEEGAIQVFKPEMGGNMLLGAVGQLQFEVVQHRLK
                     GEYDADIRLEGSQYTGARWITADTPKELQEFINAYPQRMARDAADTLAYLCTSPYDVR
                     LAQERFPKIHFHPLREHAGLALQSAG"
BASE COUNT          342 a          641 c          736 g          419 t
ORIGIN      
        1 cgccgacaaa ggcccagcgg tccagctcct gcgccaggtc gcgccggaat agttcgcgca
       61 cgatccactg cgcgccctgc catttgttga agtcgccaaa gcagccgtgg atatggatgc
      121 tgctggtggt tgtctgcatg ccggcgcgct ccagggcatc gcgcaccttc tgcacggttt
      181 ccggggtgtg gcgggtgaac tcgttgtagt caaaggccag atcggtctcg cggccgacgc
      241 tgtcgcgcga taggaagacg ccgggcactt caaacatcac ccgtgcggcg actttctgca
      301 tgcgcaggct gttggcttcg cgtgtggcgg catcctgttg gtacagtttt gatagcgcac
      361 tgcgcatatt ccgcgggggc tgcaggccat ttttatccaa aacgtaagcc acggcgccgt
      421 tctcggccac catggcatcc accggccagc gcggcccggt ggtgcccgcc atgaagggtt
      481 cacaccagcc aatgggccgc ccggtgatgg cgatgacggt cagccccgcc gctttcaggt
      541 cggccagcgc ctgcagcgca tccggagtga tggcgccatg cgtggtgagc gtgtcgtcga
      601 tgtcggtgaa cacgccaaca aggtcgcgtc ggttggaaag gggccagtgg ttcagtgcga
      661 gcacagtcgg gtggtgttgg aagcaggact ggtattgtcg cggtattgcg atgcgccagt
      721 ctggcgcaga tcaggaggtg ggtatgttcc ggtggagact ctggtgggtg ggttgggggc
      781 tgtggctgtg tgcggcgggt gctgtatctg cgcagcaagt tgcgcaggtg gtggacattc
      841 cgacgcggcc tggcgtcacg cagcgttttc tttacctggc gcctgcggca ccgcgtgccg
      901 cagtcatcct gattgccgga gggcatggcg gcctgcagat gtccactgcg ggcgatgtgc
      961 gctggggcgc cgataacttt gtggtgcgta cccgggagct gtttgcgcgc gctggcatgg
     1021 cagtggcggt ggtggatgct ccatctgacc gccagacgct gccgcacctg aacggctttc
     1081 gccagacgcc ggagcatgcc aaggacttga aagccgtcat gcagtggctg cgggccaaac
     1141 acaccgtgcc cgtgtggctg gtgggtacca gcatgggaac cctgtctgct gcgtatgtcg
     1201 gcatgacact gcccgcgcgc gacggcggac cggacggcgt ggtgttgacc tcctcggtgc
     1261 tggtggctgc cggggcgaag ggcctgcgcc ccgtgatgga gatgccggtg caacaaatca
     1321 ccatgcccgt gctggtggtg caccacacac aggatgggtg caagtattgc ccttacgacc
     1381 aggcggtcgc gctgatggac aaactgtctg cttcgccagc caaagccttg atctctattt
     1441 caggcgggca ggacaaaggc gacccctgcc acgagtgggc gtaccacggc ttcaacggca
     1501 tggaagccga aaccgtcacc cgcattgctg actggattac ggcccactaa agcgggcagg
     1561 gcgtcgtcga aaagccctca gcccgcgctc tgcagtgcca agcccgcatg ctcgcgcagc
     1621 gggtggaagt ggatcttggg aaagcgttcc tgcgccaggc gcacgtcgta cgggctggtg
     1681 cacaggtagg ccagggtgtc ggccgcgtcg cgcgccatgc gctgcgggta ggcgttgatg
     1741 aattcctgca gttccttggg ggtgtcggcc gtgatccagc gcgcaccggt gtattggctg
     1801 ccttccaggc ggatgtcggc gtcgtactcg cctttgaggc ggtgctgcac cacttcaaac
     1861 tgcaactggc ccaccgcgcc cagcagcatg ttgccaccca tctcgggttt gaacacctgg
     1921 atcgcacctt cttcgcccag ctgggccagg ccctgctgca gctgcttggt gcgcagcggg
     1981 ttcttcagca ccacggtcat gaacatttcg ggtgcaaaga acggcaggcc ggtgtacatc
     2041 aggttgacgc tgccatcggt gatggtgtcg cccagctgga cgccgccgtg ggtggtaaag
     2101 ccgacgatgt cgcccgcgta ggcctcttcc accgcctc
//
LOCUS       DAOSJM010000401         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_44697_length_2133_cov_3.317613, whole genome shotgun sequence.
ACCESSION   DAOSJM010000401 DAOSJM010000000
VERSION     DAOSJM010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44697_length_2133_cov_3.317613"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PKX82_11885"
     CDS             complement(<1..528)
                     /locus_tag="PKX82_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOF53000.1"
                     /translation="MAEATATAASAAPHKVLKVGGYTPFTATDYPGKLAAVVFVQGCP
                     WRCGYCHNPHLQSRTTDSPVPWAAVLARLCRRVGLIDAVVFSGGEPTMDPGLEEAICA
                     VRAMGFSVGLHTAGMYPQRLAAVLPLVDWVGMDIKASAHGYDAITGVAHSDAPAFASA
                     AQVLASGVAYEFRTTL"
     gene            complement(521..2095)
                     /locus_tag="PKX82_11890"
                     /pseudo
     CDS             complement(521..2095)
                     /locus_tag="PKX82_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
BASE COUNT          386 a          633 c          685 g          429 t
ORIGIN      
        1 caacgtggtg cggaattcgt acgccacgcc gctggccagc acctgcgccg cactggcaaa
       61 ggccggtgca tcgctgtggg ccacaccggt gatggcgtca tagccgtggg ccgaggcctt
      121 gatgtccatg cccacccaat ccaccagtgg caacacggca gccaggcgct gtggatacat
      181 gcctgccgtg tgcaagccca cggaaaatcc catcgcccgc acggcgcaga tggcctcttc
      241 cagaccgggg tccatggtgg gctcgccacc actgaacacc accgcatcga tgaggcccac
      301 gcgccggcac aggcgcgcca gcacagcggc ccagggtacg ggactatcgg tggtgcgact
      361 ttgcaggtgt gggttgtggc aatagccaca gcgccaggga cagccctgca caaacaccac
      421 cgccgccagc ttgccgggat agtcggtcgc ggtgaagggc gtatagccac ccaccttgag
      481 taccttgtgc ggggcagcgc tggctgcagt ggcagtggct tcagccagca agactggcct
      541 tgcagctgcc ttcggagaag taggtgcgct ccagaaactc gcccttcttg ccggtgttga
      601 acgatgccat ggggcggtgg tagcccatca cgcgggtcca tacctcgcag cgctggcgtt
      661 catcatcacg cagcgcgacc ggtgtggtct gggttgcttg cacgggggtt tgtaagtcca
      721 tcatttctat ctctcctggc ggggttggtt aaacggtttc aagctgtttg tgcgcgatga
      781 tttcggcatc gcacttggga caaaaatcgt ggcgcccgcc cagatagccg tgcttggggc
      841 aaatcgagaa ggtgggcgtg acggtgatat agggcaaacg gaagcgcgac agcgaacgct
      901 tcaccagctg acggcaggcc tcggcgctgg agagcggctc cgtcatgtac aggtgcagca
      961 cggtgccacc ggtgtatttg cgttgcaggt cgtcctgcat ctccagcgcc tcgaaggggt
     1021 cgtcggtgta acccaccggc aactggctgg agttggtgta gtagggcatg tccttggtgc
     1081 ccgcttgcaa aatatcgggg aactgcttgg cgtcttcctt ggcaaagcgg taggtggtgc
     1141 cctcggccgg ggtggcctcc aggttgtaca tgtggccggt ctggtcctgg aattcgagca
     1201 tgcgggcgcg cacatggtcc agcaggcgga tggcaaaggc gtggccccat tcggtgctga
     1261 tgtcttcacg gtcttgcgtg aaattgcgga tcatctcgtt gatgccgttc acccccagcg
     1321 tggagaaatg gttgcgcagc gtgcccagat accgcttggt gtacgggaac aggccgttgt
     1381 ccatgtggcg ctggatcacc ttgcgcttga tctccaggct gtcgcggccc agctccagca
     1441 gggtgtcgag ctctttcaac atgcccgcct catcaccttt gtgcaggtat cccaggcggg
     1501 cgcagttgat ggtgaccacc cccagcgagc cggtttgctc ggccgagcca aacaggccgt
     1561 tgccacgctt gagcagttcg cgcaaatcca gctgcaggcg gcagcacatg gagcggatca
     1621 tgttcggctt gagttccgaa ttgatgaagt tctggaaata cggcaagccg tacttggcgg
     1681 tcatttcgaa cagcagttcg gcgttcgggc tgtaccagtc gaagtcttcc gtcatgttgt
     1741 aggtcggaat cgggaaggta aacacccggc ccttggcgtc gccggccatc atcacgtcga
     1801 tataggcgcg gttgatcatg tccatctccg cctggcagtc gccataagcg aagtccactt
     1861 cctggccggc aatcaccggc acctgttccc gcaagtcttc cgggcaaatc cagtcaaacg
     1921 tcaggttggt aaacggggtc tgggtgcccc agcgggacgg cacgttcagg ttgtaaatca
     1981 gctcctgcat gcactggcgc acagccgcgt agtccatctg gtcgcgtttg acgaacggcg
     2041 ccatgtaggt gtcgaacgag ctgaaggcct gcgccccggc ccactcgttt tgcagcgtgc
     2101 ccagaaagtt gacaatctgg ccgaccgcgc tgg
//
LOCUS       DAOSJM010000402         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_45142_length_2118_cov_4.576345, whole genome shotgun sequence.
ACCESSION   DAOSJM010000402 DAOSJM010000000
VERSION     DAOSJM010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45142_length_2118_cov_4.576345"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PKX82_11895"
     CDS             <1..425
                     /locus_tag="PKX82_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOF53001.1"
                     /translation="TGASNVIPGDVVIDFNFRFSTESTPESLQSRLTKVLQKHGLEFD
                     LAWTIGGLPFLTTPGPLVDAVREAILAETGVQTQLSTTGGTSDGRFIAQICPQVIEFG
                     PPNATIHKINEHVAVSDIEPLKNIYRRVLENLNQQASA"
     gene            422..1309
                     /gene="prmB"
                     /locus_tag="PKX82_11900"
     CDS             422..1309
                     /gene="prmB"
                     /locus_tag="PKX82_11900"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOF53002.1"
                     /translation="MTLLELIESSAQRLTDAGVSFGHGTTNAFDEAAWLVLHSLGLPL
                     DTQLTGENSEGNRPLAPADIAQAATLLEARITTRKPAAYLTHEAWLQGVAFYVDERVI
                     VPRSLIAELLVDGSIDYWLKESTHRVLDLCTGNGSLAVIAAMVYPDVVVDGADISRDA
                     LDVARINVERHGLGERIRLLESDGLAGAMGPYDLILCNPPYVNAQSMSELPPEYLAEP
                     FIALDGNQQGGTDGMDFIRKLLQQAPAHMAPDAVLVLEIGNERPYFEAAFPSLEVVWL
                     ETSAGDDQVLLITRESLLK"
     gene            1306..>2118
                     /locus_tag="PKX82_11905"
     CDS             1306..>2118
                     /locus_tag="PKX82_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF53003.1"
                     /translation="MITLKNVTLRRSAKVLLEGASVTLNPGEKVGLVGRNGAGKSSLF
                     ALFNGNLHEDAGEYYIPAQWRMGQVAQDMPETDQSATDFVIEGDTVLNAARQEVAAAE
                     ATDDYDRMAHAYTGLHDAGEYDAAARAQALILGLGFKTTELTNPVNSFSGGWRMRLQL
                     ARALMCPSDLLLLDEPTNHLDLDALVWLEAWLKKYEGTMIVISHDREFLDAITNVTLH
                     IDAGKLVRYGGNYSKFEDMRAEQMELQQATVAKQAEKIAHLQSFIARFKAKAS"
BASE COUNT          439 a          654 c          629 g          396 t
ORIGIN      
        1 gcacgggggc gagcaacgtc attccgggtg acgtcgtgat tgacttcaat ttccgctttt
       61 ccaccgagtc cacgccagag tccctgcaaa gccggctcac caaggtcctg caaaaacacg
      121 gtctggaatt cgatctggcc tggaccattg gtggcctgcc atttctcaca acccctggtc
      181 cgttggtgga cgcagtgcgc gaggccatcc tggctgaaac cggcgtgcag acccagctct
      241 ccaccaccgg cggcaccagt gacggacggt tcatcgcgca gatctgcccc caggtgatcg
      301 aattcggccc gcccaatgcc accatccaca agatcaacga gcatgtggcg gtgtcggaca
      361 ttgagcccct taaaaacatt taccgccggg tgctggaaaa cctgaatcag caggccagcg
      421 catgaccctg cttgagttga ttgaatcgtc cgcacaacgg ctgaccgatg ccggtgtgtc
      481 gtttggccat ggcacgacca acgccttcga cgaagccgca tggctggtac tgcacagcct
      541 gggattgccg ctggacacac agcttaccgg cgagaattct gaaggaaata ggccgttagc
      601 ccccgccgat attgcacaag ccgctacact tttggaagcg cgcatcacca cgcgcaaacc
      661 cgcagcctac ctgacccacg aggcttggct gcagggtgtt gcgttctatg tggacgagcg
      721 ggtcatcgtt ccgcgctcgc tgattgccga gttgctggtg gacggcagca ttgactactg
      781 gctcaaggaa tccacgcacc gcgtgctgga cctgtgcacc ggcaacggca gcctcgctgt
      841 gatagccgcc atggtctacc ccgatgtcgt ggtggacggg gccgatatat cgcgtgacgc
      901 gctggatgtg gcccgcatca atgtggagcg tcatgggctg ggcgagcgca tccggctgct
      961 ggagtccgat ggccttgctg gagcgatggg gccgtacgac ctgatcctgt gcaacccgcc
     1021 ctacgtcaat gcccagagca tgtcggaact gccgcccgaa tacctggcgg agcctttcat
     1081 cgcgctggat ggcaaccagc aaggcggcac ggacggcatg gactttatcc gtaaactgtt
     1141 acaacaggct ccggcacata tggcacctga cgcggtgctg gtgctcgaaa ttggcaatga
     1201 acgcccctac tttgaagccg catttccatc cctggaagtg gtctggctgg aaaccagcgc
     1261 tggtgacgac caggtcttgc tcatcacgcg tgaatcgctc ctgaaatgat tactttgaaa
     1321 aatgtcacct tgcgccgcag tgccaaggtg ttgctggagg gtgcctccgt cacgctcaat
     1381 ccgggcgaaa aagttggttt ggtgggacgc aatggcgccg gcaagtcttc gttatttgcc
     1441 ctgttcaacg gaaacctgca tgaagatgcg ggcgagtact acatccctgc ccagtggcgc
     1501 atgggccagg ttgcccagga catgccggaa acggaccaga gcgccaccga tttcgtgatc
     1561 gagggggaca cggtgctcaa tgctgcacgc caggaagtgg cggcggccga agccaccgac
     1621 gactatgacc gcatggccca cgcctacacc gggctgcatg atgccggcga atacgacgcg
     1681 gccgcccgcg cgcaggccct cattttgggt ctgggcttca agaccactga actgaccaac
     1741 ccggtgaaca gcttctccgg tggctggcgc atgcgcctgc aactggcacg cgcactgatg
     1801 tgcccgagcg acctgctgtt gctggacgag cccaccaacc acctggacct cgacgccctg
     1861 gtctggctgg aggcctggct caagaagtac gaaggcacga tgattgtcat cagccacgac
     1921 cgcgagtttc tggatgcaat caccaacgtc acgctgcaca ttgatgccgg taagctggtg
     1981 cgctatggcg gcaactacag caagttcgaa gacatgcgcg ccgagcagat ggagctgcaa
     2041 caggccacgg tagccaagca agccgagaaa atcgcccacc tgcaatcctt catcgcccgc
     2101 ttcaaggcca aggcgagc
//
LOCUS       DAOSJM010000403         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_45578_length_2104_cov_3.357247, whole genome shotgun sequence.
ACCESSION   DAOSJM010000403 DAOSJM010000000
VERSION     DAOSJM010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45578_length_2104_cov_3.357247"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..997
                     /locus_tag="PKX82_11910"
     CDS             161..997
                     /locus_tag="PKX82_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HOF53004.1"
                     /translation="MPRFAANLTMLYPELPFLARFEAAARDGFKAVEFLFPYAWSKQE
                     LVANLTGNGLQLVLMNAPPGGTDAASSMQAWEAGSRGLACIPGREAEFRRGLQLGLDY
                     CAALGCPSLHVMSGVLPDGLERETLLTVMVANLRWATQRAQQAQVTLLIEPINLRDMP
                     RYFLNRQDHAHEILTAVNTSNLQVQMDLYHCQIVEGDVSTKLRQYLPTGRIGHMQIAG
                     VPERHEPDVGELNYPYLFKLIDALGYTGWLGCEYRPQRGAKPGGTSEGLGWLKPWLGA
                     QE"
     gene            1400..>2104
                     /locus_tag="PKX82_11915"
     CDS             1400..>2104
                     /locus_tag="PKX82_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOF53005.1"
                     /translation="MNARIPSTILSAPQALSQISQAWDDDIVGQLTDYIAIPAKSPMF
                     DADWAQHGYIDTVVRNAANWVQAQKVQGLTLEVIRLPGRTPVLFFEVPASSGAIESEA
                     CITDSSRASGETVMMYGHLDKQPEFSGWRSDLGPWSPKYQDGKLYGRGGADDGYAIYA
                     AIAAIQALKSQNVAHPRIVGLVETCEESGSYDLLPYIDTLRERLGEVGLVICLDSGAG
                     NYDQLWLTNSLRGMASG"
BASE COUNT          412 a          605 c          633 g          454 t
ORIGIN      
        1 cggtaggcac aaacgccagc gttccaggtg ctatggaatt gatagctgtc tgcgaagtct
       61 gcataagcgc ttgaaggcta ttttgttgat atgaatgaaa aatggaagct tgttgcctgt
      121 gcgacacttt acgcctgtta ttacccgatc agatttcacc atgccccgtt ttgctgccaa
      181 cctcaccatg ctttacccgg agctgccctt tctggcacgt tttgaggcgg ccgcgcgtga
      241 tggtttcaaa gcagtcgagt tcctgtttcc ctatgcctgg tccaagcagg aactggtggc
      301 gaacctgaca ggcaacggat tgcagctggt gctgatgaat gcaccgccgg gtggcacaga
      361 tgctgcgtca agtatgcagg catgggaagc cggaagccgg gggctggcct gcattcccgg
      421 gcgcgaggcc gagttccgtc gtggcctgca actgggcctg gactactgcg ccgcactggg
      481 ctgcccgagc ttgcatgtga tgtcgggtgt gttgcccgat ggtctggagc gtgaaaccct
      541 gctgacggtg atggttgcca acctgcgctg ggcgacccag agggcgcaac aggcgcaggt
      601 caccttgctg atcgagccca tcaacctgcg tgacatgccg cgatatttcc tcaaccggca
      661 agaccatgca cacgagattc tgaccgctgt caacacctcc aatctgcagg tgcagatgga
      721 cctgtaccac tgccagattg ttgaaggcga cgtttccacc aagctgcgcc agtacctgcc
      781 caccgggcgc attggtcaca tgcagattgc cggcgtgccc gagcgccatg agcccgatgt
      841 gggcgaactc aattacccgt atcttttcaa gctgattgac gcactgggct acaccggctg
      901 gctgggttgt gaataccggc cacagcgggg tgcaaagccg gggggcacct cggagggctt
      961 ggggtggttg aaaccctggc ttggtgcgca agaatagcgg gtcccacagt agtcccaccg
     1021 aagccatttt ctctgactgc agcgactggc tctgcgcctc gctcagtttg ctgttgagtt
     1081 cagacaacgc gcgcacctgg gcctgcatgg caacttcaat gtgccgacgc tccgagatgt
     1141 ctctggagat gccgaaagta ccaatggatc gcccatcccg gtcacgcagg ggcaccttgg
     1201 ttgttgaaac ccaggtctca ggcttgtctt gccacgtttc tttctcctcg atcgtcaagg
     1261 ccttcccggt ccggatattg gccagttcat ccaggcgcgc ctgttgcgcg tgcgtcccag
     1321 gggttacaga catcgggatg cggtgacagc tgggtcgtgg catgatcaaa tcacgaatcc
     1381 attgcccaag gagttttgta tgaatgcccg catccctagt accattctga gcgcccccca
     1441 agccctgagc cagatcagcc aggcgtggga cgatgacatt gttggccagc tcacggacta
     1501 catcgccatt cctgccaagt cccccatgtt cgatgccgac tgggcgcagc acggctatat
     1561 cgacaccgtg gtgcgcaatg cggccaactg ggtacaggcg caaaaagtgc agggcctgac
     1621 gctggaagtg attcgtctgc caggacgaac gccggtgctg ttctttgaag ttcctgcctc
     1681 ctcaggtgct attgaatcag aagcttgtat tacagattca tcaagggcta gcggcgaaac
     1741 agtcatgatg tatgggcatc tggacaaaca gcccgagttt tccggctggc gctctgacct
     1801 ggggccgtgg agtcccaaat accaggatgg caagctgtat ggccgggggg gtgcggacga
     1861 cggctatgcc atctatgccg ccattgctgc catccaggcg ctgaagagcc aaaacgtggc
     1921 gcacccgcgc attgtgggcc tggtcgaaac ctgcgaggaa agtggctcct atgacctgct
     1981 gccctacatc gacaccttgc gcgagcgact gggtgaggtc ggtctggtga tctgcctgga
     2041 ttcaggtgct ggcaactatg accagctgtg gctcaccaac agcctgcgcg gcatggccag
     2101 cggt
//
LOCUS       DAOSJM010000404         2103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_45612_length_2103_cov_3.626953, whole genome shotgun sequence.
ACCESSION   DAOSJM010000404 DAOSJM010000000
VERSION     DAOSJM010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45612_length_2103_cov_3.626953"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKX82_11920"
     CDS             complement(<1..451)
                     /locus_tag="PKX82_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOF53006.1"
                     /translation="MAVTEQAIMAALQGVVDTNTGKDFVSSKCIRNFSVGDGDVSFDV
                     ELGYPAKSQIPEFRKALVAAAKSVSGVSNVSVNITSKVLAHAVQRGVQLLPNVKNVVA
                     VASGKGGVGKSTTAVNLALALAAEGASVGILDADIYGPSVPMMMGIDG"
     gene            593..1870
                     /locus_tag="PKX82_11925"
     CDS             593..1870
                     /locus_tag="PKX82_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOF53007.1"
                     /translation="MASTPAQPSSTGLLLTGGGARAAYQVGVLEAIADMRKECGAGND
                     PNPFPIITGTSAGAINASALACGADDFDGTVRLIAKTWREFHAHDVYRSDHLSMLRSG
                     ARWMTLLSVGWIVAKFRRIKPKSLLDNSPLGELLQRLVPLERLPELVRQGHMQALAVT
                     ASSYSSGDHITFFEGDKRLMPWVRTQRLAVRDKITHAHLLASSAIPFVFPATELHING
                     HTEYFGDGSMRQSAPIAPAIHLGAEKILVVGAGRMHEPKNEAHLHTTNTYPSIAQIAG
                     HALSNIFLDALAVDVERVRRINQTIGLVPPEARKASALRPVDLLVIAPSQRLDAVASR
                     HIGDLPHAVRTMLGGVGVSSAKADMKGSALASYLLFESGYTSELMALGYADAQKQRAE
                     VCKFFGWTDPEALLPIAPAKPFVERRSDPLRLR"
BASE COUNT          380 a          733 c          590 g          400 t
ORIGIN      
        1 gtccgtcaat gcccatcatc atgggcacgc tggggccata aatgtcggcg tccagaatac
       61 ccacactggc gccctcggcc gccagtgcca gcgccagatt cacggctgtg gtgctcttgc
      121 ctacaccgcc cttgcccgat gccaccgcca ccacattctt cacattgggc agcagctgca
      181 cgccacgctg caccgcatgg gccagcacct tgctggtgat attgaccgac acattggaca
      241 cgccggaaac gctcttggcc gctgccacca atgccttgcg aaactcgggg atctggctct
      301 tggcgggata gcccagctcc acatcaaacg acacgtcgcc atcgcccacg ctgaaattgc
      361 ggatgcattt cgaactgacg aaatccttcc cggtattggt atccaccacg ccctgtaggg
      421 ccgccatgat tgcctgttca gtcactgcca ttcaaatctc ctgttcccct gagtctactg
      481 aaccccggca atcccgcaga gggtgtaagt ccaatcgcgc cgctaatcgc taggggttta
      541 ccccgttttt ccagccgttc cagcggtgca tcgaggttcc ataatggatc gcatggcaag
      601 tacccccgca caacccagca gcacaggact cttgttgacc ggcggcggcg cacgcgcggc
      661 ctaccaggtc ggtgtactgg aggcgattgc ggacatgcgt aaggaatgtg gggccggcaa
      721 tgaccccaac ccctttccca tcatcaccgg aacctctgcc ggggcgatca acgcctctgc
      781 gctggcctgc ggtgcggatg actttgatgg caccgtgcgc ctcattgcca agacctggcg
      841 cgaatttcac gcccacgacg tctaccgctc cgaccacctg agcatgctgc gctccggcgc
      901 gcgctggatg acgctgctct cggtgggctg gatcgtcgcc aagttccgcc gcatcaagcc
      961 caagtccctg ctggacaact ctcctctggg agaactcctg cagcgcctgg tcccgctgga
     1021 gcgtttgccc gaactggtgc gccagggcca catgcaggca ctcgcggtca cggcatcgag
     1081 ctacagctcg ggtgaccaca ttacgttttt tgagggcgac aaacgcctca tgccctgggt
     1141 gcggacccaa cggctggcgg tgcgcgacaa gatcacccat gcacacctgc tggcctccag
     1201 cgccatcccg tttgtctttc cggccacgga gctgcacatc aacggccaca ccgaatactt
     1261 tggcgatggc tccatgcggc aatcggcccc gatcgcaccc gctattcatt tgggagcaga
     1321 aaagatcctg gtggtggggg ctggacgcat gcacgagccc aaaaacgaag cccacctgca
     1381 taccaccaac acctacccca gcattgcgca gattgccggt cacgccctgt ccaacatctt
     1441 tctggacgcg ctggccgtgg atgtggagcg ggtgcgccgc atcaaccaga ccattggcct
     1501 ggtgccgcca gaagcgcgca aggccagtgc cctgcggccg gtggacctgc tggtgatcgc
     1561 acccagccag cgcctggatg ctgttgcgtc ccgccacatc ggtgacctgc cccacgcggt
     1621 gcgcaccatg ctcggtggcg tgggcgtttc gtctgccaaa gccgacatga aaggctccgc
     1681 gctggccagc tacctgttgt tcgagtccgg gtataccagc gagctgatgg ccctgggcta
     1741 tgccgacgcc cagaaacagc gggctgaggt gtgcaagttc ttcggctgga ctgacccgga
     1801 agccttgctc cccattgcgc cggccaaacc gtttgtggag cggcgcagcg accccttgcg
     1861 gctgcgctga cggtctgtgc ggcaagggac caggtgggct gggcgctttg ctccgtgtcc
     1921 cccgcccgct gcgcgggctc ctcctttacc tgcgcaaaac acccagcccg cctggtcctc
     1981 cctgtactgg ggcgactaaa atcaccagtt cctctgtgaa agtcgttccc atgccccgcc
     2041 aactctttgt caccaccgcc ctgccctacg ccaacggcaa cttccacatc ggccacatca
     2101 tgg
//
LOCUS       DAOSJM010000405         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_45821_length_2096_cov_3.675649, whole genome shotgun sequence.
ACCESSION   DAOSJM010000405 DAOSJM010000000
VERSION     DAOSJM010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45821_length_2096_cov_3.675649"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PKX82_11930"
     CDS             <1..337
                     /locus_tag="PKX82_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybenzoate 4-monooxygenase"
                     /protein_id="HOF53008.1"
                     /translation="SPLRRHTAPGEDVDAVIDTRAVFQQGFRDLDYAAMPTLLRPHVG
                     RYGLCDYEKVFCVDAKRGEDIFASRGIDKSAGCMVLVRPDQYVGHILPLHARAELAAY
                     FAAILRVRG"
     gene            complement(334..>2096)
                     /locus_tag="PKX82_11935"
     CDS             complement(334..>2096)
                     /locus_tag="PKX82_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOF53009.1"
                     /translation="PDIARTDYLLVLGANPLASNGSMWTVPDFKGKAKALQVRGGKLV
                     VIDPRRTETAAMADAHHFIRPGADVFLLAAMVHTLFAEKLVSLGTVSEWVVGVDAVQQ
                     AVAPFTPEAVATRCGISADTIRSLARTLASTPRAAVYGRIGTCTQQYGTLASWLIDVL
                     NTLTGHLDVPGGMLFAKSAAFGQNTAGNPANKPGVGKGVSTGRHHARVSGAPEVYGEL
                     PMTCLSEEIETPGEGQIKALFTVATNPVLSSPDGPRLAKALDTLEFMVSMDIYLNETT
                     RHADVILPGVSPLEDLHYDVAFPQLSWRNHARYSPPVFERAPDQPEEWQTMLKLAAIV
                     QGKGAAVDANALDDAQFAEDAQRLFGQHADAVIAATAHYKGPQRSLDMALRAGPYGDQ
                     FGLQPDGLNLAKVMASNHAGGIDLGELQPRIPEMLRTPSGKVELAPPSLLQDLQRAAQ
                     DVRGLAPDLVIIGRRDVRTNNSWMHNLPILAKGPFRCTALVHPQDAARLGLQDGAMAW
                     LHNGPRSVQAQVHISDEMMPGVVSLPHGWGHDLPGAQLQVAAERPGANLNALLDDQLR
                     DPLSGNAVLGGVAITMTAAI"
BASE COUNT          331 a          659 c          715 g          391 t
ORIGIN      
        1 ttcacccctg cgccgccaca ccgctccagg agaagacgtg gatgccgtga tcgacacccg
       61 cgcggtcttc cagcagggat tccgcgatct ggactacgcc gccatgccca cgctgttgcg
      121 cccgcacgtg gggcgctacg gactgtgtga ctacgagaaa gtattctgtg tcgacgccaa
      181 gcgtggcgag gacatctttg cgtcgcgtgg catcgacaag tccgcgggct gcatggtcct
      241 cgtgcgcccg gaccagtacg ttggccacat cttgccattg cacgcgcgtg ctgaactggc
      301 ggcctatttc gccgccatcc tgcgtgtgcg tggctagatt gccgctgtca tcgtgattgc
      361 cacaccgccc agcaccgcgt tgcccgagag cgggtcgcgc agctggtcgt ccagcagggc
      421 gttgaggttg gcgccgggcc gctcggcggc cacctgcagt tgtgcgccag gcaagtcgtg
      481 gccccagccg tgcggcagac tgaccacccc cggcatcatc tcgtcgctga tgtggacctg
      541 ggcctgcacg ctgcgcgggc cgttgtgcag ccaggccatg gcgccgtcct gaagccccag
      601 ccgggcggca tcctgcgggt gaaccagcgc agtgcagcgg aacgggccct tggccaggat
      661 gggcaggttg tgcatccagc tgttgttggt gcgcacgtcg cgcctgccaa taatgaccaa
      721 atcgggcgct agccctcgta catcttgcgc agcgcgctgc aaatcttgta gcagactggg
      781 tggggccagc tccaccttgc cactcggtgt gcgcagcatt tcgggaatgc gtggctgcag
      841 ttcgcccagg tcgatgccgc cggcatggtt ggacgccatc accttggcca ggttcaggcc
      901 atcgggttgg aggccaaact ggtctccgta aggtccagcg cgcagtgcca tgtccaggct
      961 gcgctgcggt cccttgtagt gggcagtggc ggcaatgacc gcatcggcat gttggccaaa
     1021 caggcgctgc gcgtcttcgg caaactgggc gtcgtccagc gcgttggcat ccaccgccgc
     1081 gcccttgccc tgcacgatgg cagcgagttt gagcatggtt tgccactctt cgggctggtc
     1141 tggcgcgcgc tcaaacaccg gtgggctgta gcgtgcatgg ttgcgccagg agagctgggg
     1201 aaaggcgacg tcgtagtgca ggtcttccag tggcgatacg ccgggcagga tcacatccgc
     1261 atggcgcgtg gtctcgttga ggtagatgtc catgctgacc atgaactcca gtgtgtcgag
     1321 ggccttggcc aggcgggggc cgtccgggct ggagagtacc gggttggtgg ccacggtgaa
     1381 cagggccttg atctggccct ctccaggggt ttctatttct tcgctcaggc aggtcatggg
     1441 cagctcgcca tacacctcgg gtgcgccgct gacgcgcgca tggtggcgtc cggtggacac
     1501 gcctttgcca acacctggtt tattcgcggg gttgccagcg gtgttctggc cgaaagcggc
     1561 ggacttggca aacagcatgc cgccggggac atccagatgg ccggtcaagg tgttgagcac
     1621 atcgatcagc cagctcgcca gtgtgccgta ctgctgggtg caggtaccaa tgcggccata
     1681 gaccgcggcg cgcggtgtgg acgccagcgt acgcgccagc gagcggatgg tgtcagccga
     1741 tatgccgcaa cgtgtggcta cagcttcggg tgtgaagggg gccaccgcct gctgcacggc
     1801 atcgacgccc accacccact cagacaccgt gcccagtgag accagctttt cggcaaacag
     1861 ggtgtgcacc atggccgcaa gcaggaacac gtcggcacca gggcggatga agtggtgcgc
     1921 atcggccatg gccgcagtct cggtgcgccg ggggtcaatc accaccagct tgccgccgcg
     1981 tacctgcagg gctttggcct tgcccttgaa gtcgggcacg gtccacatgc tgccgttgct
     2041 ggccagcggg ttggccccca gcaccagcag gtaatcggtg cgggcaatgt cgggca
//
LOCUS       DAOSJM010000406         2089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_46058_length_2089_cov_3.109636, whole genome shotgun sequence.
ACCESSION   DAOSJM010000406 DAOSJM010000000
VERSION     DAOSJM010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46058_length_2089_cov_3.109636"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKX82_11940"
     CDS             complement(<1..400)
                     /locus_tag="PKX82_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOF53010.1"
                     /translation="MFAIKKRAVRALLTGATALFFINSAGAGIPIVHWTQPSGAQVFL
                     VESPGIAMVDVRLEFDAGSRRDPTDKPGLASLMASSTTYGVRATPESPALDENSLSDA
                     WADLGAAFGGNASSDRLGFSLRSLTYPDILP"
     gene            complement(431..1792)
                     /locus_tag="PKX82_11945"
     CDS             complement(431..1792)
                     /locus_tag="PKX82_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOF53011.1"
                     /translation="MSTAASSAQDAGVQQFTLKNGMSLIVKPDHRAPTAVHMVWVRVG
                     SMDEVDGTSGVAHVLEHMMFKGTPTVKAGDFSRKVAALGGRENAFTSKDYTGYFQQIP
                     AAKLEDVMRLEADRFAYNQWSDEDFRKELEVVKEERRMRTEDNPHARLHEAMDATIYQ
                     AAPYRRPIVGWMSDLEAMTPDDARAFYKRWYTPTNATVVVAGDVQVPQVRAWAEKYYG
                     SLPTRAVPARKPREEPAQSGLRRIDFKAPAEQSYVALAFKVPGFAPAALDPAKADAAS
                     DDALALTVLSAVLAGYDNARLERALTQGDDPVADSAGAYCGMAARGPQLCTLYGVPAT
                     GKTAVQVEQALRAQVARIAADGVSEAELQRVKNQWVAGEVYKQDSVFNQARILGVHWI
                     NGFPLDADERLIARLRAITAAQVQSVAGRYFGDDALTVATLLPQPVDKKRKPRTPAAG
                     ARH"
     gene            1888..>2089
                     /locus_tag="PKX82_11950"
     CDS             1888..>2089
                     /locus_tag="PKX82_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOF53012.1"
                     /translation="MFSFFKKKSPPPPAATAPEPVAGTPSAAPATPPAAPAPLPQTGS
                     LIGSALLAPIEIPVAPATPPERQ"
BASE COUNT          361 a          698 c          655 g          375 t
ORIGIN      
        1 ttggcaggat gtccgggtag gtcagcgagc gcagcgaaaa acccagacgg tcggaactgg
       61 cgttgccacc aaacgcagcc cccagatcgg cccaggcatc gctcaagctg ttctcatcga
      121 gtgcaggact ctcgggcgtt gcccgcacgc cataggtcgt ggacgaggcc atcaggctgg
      181 ccagacccgg tttgtcggtc ggatcgcggc ggcttccggc atcgaactcc aggcgcacat
      241 ccaccatggc gatgcccggg ctttccacca gaaacacctg tgcacccgac ggctgcgtcc
      301 agtgcacgat gggaatgcca gcaccggcag aatttatgaa aaacagggct gtagcccccg
      361 tcaatagtgc gcgaacagct ctttttttga tagcaaacat ggtgtctttc tggcggctcg
      421 gtggcgtgaa tcagtggcgc gcaccggcgg ccggcgtgcg gggtttgcgc ttcttgtcca
      481 cgggctgcgg cagcaatgtg gccaccgtga gcgcgtcgtc gccaaagtag cggccggcca
      541 cagactgcac ttgcgccgcg gtgatcgcgc gcagccgggc aatcaggcgt tcgtcggcgt
      601 ccagcggaaa accgttgatc cagtgcacac ccagaatgcg ggcctggttg aagacgctgt
      661 cctgcttgta gacctcgcct gccacccact ggtttttgac acgctgcagt tcggcttccg
      721 acacgccatc ggccgcaatc cgcgccacct gagcgcgcag ggcctgctcc acctggaccg
      781 ccgtcttgcc tgtcgcgggg acaccataca aggtacacaa ctgcggcccg cgcgcggcca
      841 tgccgcagta ggcgccggca ctgtcagcca ccggatcatc accctgggtg agcgcccgct
      901 ccagccgcgc gttgtcgtag cccgcgagca ccgccgatag cacggtcaaa gcgagtgcat
      961 cgtcgctggc agcatcggct ttggccgggt ccagtgcggc gggcgcaaaa ccgggcacct
     1021 tgaaagccag cgccacgtag gactgctcgg caggtgcctt gaagtcgatg cggcgcaggc
     1081 cactctgggc gggttcttcc cgtggcttgc gggcgggcac agcgcgggtc ggcaggctac
     1141 cgtagtactt ttcggcccag gcacggacct gcggcacctg gacatcaccc gccaccacca
     1201 cggtggcatt ggtgggggtg taccagcgct tgtaaaaagc gcgggcgtcg tcgggcgtca
     1261 tggcttcgag gtcgctcatc cagcccacga tggggcgacg gtacggtgcg gcctggtaga
     1321 tggtggcgtc catggcctcg tgcaggcggg catggggatt gtcttcggtc cgcatgcggc
     1381 gctcttcctt gacgacctcc agctccttgc ggaagtcttc atcgctccac tggttgtagg
     1441 caaaccggtc tgcctccagt cgcatcacgt cttcgagttt ggccgccgga atctgctgaa
     1501 aatagccggt gtagtccttg ctggtgaagg cgttctcgcg tccacccagc gccgccacct
     1561 tgcgggagaa gtcgcccgcc ttcacggtcg gtgtgccctt gaacatcatg tgctccagca
     1621 catgggccac gccactggtg ccgtccacct cgtccatgga gcccacgcgc acccacacca
     1681 tgtgcaccgc cgtcggtgcg cggtggtcgg gtttcacgat gagggacatg ccgttcttta
     1741 atgtgaactg ctgcacaccc gcgtcctgcg cgctgctggc agccgttgac atcagggaag
     1801 aacagaggag ggttgcggcc caaatagcgc gcttcagccg ggggacaagg tgtttcatag
     1861 aatgctgtga ttctaagaac gccccacatg ttcagctttt tcaagaaaaa atcacctccc
     1921 ccccctgcgg ctactgcgcc agagccagta gcaggcacgc catcggccgc ccctgccacg
     1981 ccgccagcag cccccgcccc cctgccgcaa acgggctcgc tgattggcag cgcactgctg
     2041 gcgcccatcg agatccccgt cgcccctgcc acaccaccgg agcggcaga
//
LOCUS       DAOSJM010000407         2083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_46246_length_2083_cov_2.425049, whole genome shotgun sequence.
ACCESSION   DAOSJM010000407 DAOSJM010000000
VERSION     DAOSJM010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46246_length_2083_cov_2.425049"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="PKX82_11955"
     CDS             <1..485
                     /locus_tag="PKX82_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOF53013.1"
                     /translation="LRDAGMAKVAVLDIDYHHGNGTQAIFYDRADVFFASIHGDPRTE
                     YPFYLGHADERGTGAGLGTNLNIPLPRGTGFDVWAQALDTALATIAQFGADALVVSLG
                     MDTFVGDPISGFALQSADYLRVGERIAVAGLPTAWVFEGGYAVAEVGVNAVNVLEGFA
                     "
     gene            494..1159
                     /locus_tag="PKX82_11960"
     CDS             494..1159
                     /locus_tag="PKX82_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOF53014.1"
                     /translation="MIQLHYHPSDASLMPHIFLEELGVPFELLLVDRSCNAQKSPEYL
                     RLNPNGLIPVLQDGSLVLYETPAILLHLSDTHPQSQLLPPVGTHERAHAYKWLVWLSN
                     TLQTQLVQYFYAERYVQSSGVADFRARAEARLAPLLDQVEAQLQAGGGPWLLGAHYSA
                     ADCMAFVLCCWTRNLAHPASQRPAIGAYLARMRQRPAVQRAVSTEQLPESFFVRAASP
                     SSH"
     gene            complement(1160..1987)
                     /locus_tag="PKX82_11965"
     CDS             complement(1160..1987)
                     /locus_tag="PKX82_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HOF53015.1"
                     /translation="MLPLSDWPLDERRGIRGVFTDIDDTLTTHGAITPDALAALGALR
                     AAGLTVIAITGRPVGWSEPKARAWPVDAIVAENGAVALVSDQNSLIPSSEGRQTLSKI
                     YQLDAATRQAHTRRLRQAAERVLREVSGVSLARDSAGRETDIAFDHAEFASLTATQID
                     AIEAVLRAEGLHTTRSSIHTHGAVSPGDKWTGAVWAVRTLLGRDLTQELDCWVAVGDS
                     GNDQAMFAHFTHSVGVANIRKFTDQLTHLPRFISPSAHGAGFAEVARAVLQGRAPGG"
BASE COUNT          330 a          673 c          688 g          392 t
ORIGIN      
        1 atttgcgtga tgccggcatg gccaaggtgg ccgtgctgga catcgactac caccacggca
       61 acggtacgca ggccattttc tacgaccgtg ccgacgtgtt ctttgccagc atccacggcg
      121 acccgcgcac cgaatacccg ttctatctgg gccatgccga cgaacgcggc accggcgcgg
      181 ggctgggcac caacctcaac ataccgctgc cgcgcggcac cgggtttgac gtttgggcac
      241 aagcgttgga caccgcgttg gcgaccatcg cccaatttgg cgccgatgca ctggtggtgt
      301 cgctgggcat ggacacgttt gtgggcgacc ccatttccgg cttcgcgctg caaagtgcgg
      361 actacctgcg cgtgggcgag cgcattgcgg tggcgggttt gcccacagcc tgggtgttcg
      421 aaggtggtta cgccgtggcc gaggtcggcg tgaacgcggt caacgtgctg gaagggtttg
      481 cataatgttg gccatgatcc aactgcatta ccaccccagc gacgccagtc tgatgcccca
      541 cattttcttg gaggaactgg gcgtgccgtt cgagctattg ctggtagacc gttcgtgcaa
      601 tgcacaaaag tctccagaat atctgcggct caatcccaat gggctcatcc ctgtgctgca
      661 agacggtagc ctggttttgt acgagacgcc tgccatcctc ttgcatctat ctgacaccca
      721 tccgcaatcg caattgctgc cacccgtcgg tacccacgag cgcgcccatg cgtacaagtg
      781 gctggtgtgg ttgtccaata ccttgcaaac ccagttggtg cagtatttct atgccgagcg
      841 gtatgtgcag agcagtggcg ttgccgattt tcgggcgcgt gcggaggcac ggctcgcgcc
      901 gctgctcgac caagtcgaag cacaactgca ggccggtggt ggaccgtggc tgctgggcgc
      961 gcactacagc gccgccgact gcatggcttt tgtgctgtgt tgctggacgc gtaacctggc
     1021 acatccggca agccagcgtc cggccatcgg tgcctatttg gcccgcatgc gccaacgccc
     1081 ggcagttcaa cgcgccgtca gcaccgagca gttgcccgag tcattctttg tccgcgcggc
     1141 gtccccgtcg agccattgat cagccgcccg gtgcacggcc ctgcagaacg gcgcgcgcca
     1201 cttcggcaaa tccagcgcca tgtgcgctgg ggctgataaa gcggggcagg tgggtgagct
     1261 gatcggtaaa cttgcggatg ttggccacgc ccacgctgtg ggtgaagtgc gcgaacatgg
     1321 cttggtcgtt gccggagtcg cccacggcta cccagcaatc cagctcctgt gtcaggtcgc
     1381 gtccgagcaa ggtgcgcacc gcccacacgg cgccggtcca tttgtcgccc gggctcaccg
     1441 cgccgtgggt gtggatgctg ctgcgcgtgg tgtgcaggcc ctcggcgcgc agcacggcct
     1501 cgatggcatc gatctgcgtg gcggtgagtg aggcgaactc ggcgtggtcg aaggcgatgt
     1561 cggtttcgcg ccccgccgaa tcccgcgcca gactcacgcc ggacacctcg cgcagcacgc
     1621 gctcggcggc ctggcgcagg cgccgggtgt gcgcctgccg cgttgcggcg tccagctggt
     1681 atatttttga tagcgtctgg cgaccttccg acgatgggat gaggctgttc tggtctgaaa
     1741 ccagggccac ggcaccgttt tcggccacga tggcgtccac cggccaggcg cgggccttgg
     1801 gttcggacca tccgaccggc cgcccggtga tcgcgatgac cgtcagcccg gccgcacgca
     1861 acgcccccag cgcggccagc gcatccggcg tgatggcgcc gtgcgtggtc agcgtgtcgt
     1921 cgatgtcggt gaacacgccg cggatgccgc ggcgctcgtc cagcggccag tcggacaggg
     1981 gcaacatcag cctgcgctgc ccagcgccag ccccgcatgc tcgcgcagcg ggtggaaatg
     2041 gatcttcggg aaacgctcct gcgccaggcg cacgtcgtag ggg
//
LOCUS       DAOSJM010000408         2069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_46642_length_2069_cov_5.562562, whole genome shotgun sequence.
ACCESSION   DAOSJM010000408 DAOSJM010000000
VERSION     DAOSJM010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46642_length_2069_cov_5.562562"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="PKX82_11970"
     CDS             complement(<1..1021)
                     /locus_tag="PKX82_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOF53016.1"
                     /translation="MSSAPSSPASVTHGLNLAQQEAVNYLHGPCLVLAGAGSGKTRVI
                     THKIARLIQSGMPANRIAAITFTNKAAAEMRERAKGLIGKAAKDVQICTFHALGVRML
                     RQDGSVLGLKPAFSILDTDDVTSILKDAGGTVDAATARQWQWTISGWKSAGLSSAQAL
                     ALAADDNERIIATVMGRYEERLTAYQSVDFDDLISLPLKLLQNHEQVRQKWQGLMGHI
                     LVDEYQDTNATQYEMLKLLAGGNPASSARFTAVGDDDQSIYGWRGATLDNLKKLPIDF
                     PTLKLVKLEQNYRSTSAILRAANNVIGPNPKLFPKTLFSELGEGEPVRVVDADNEEHE
                     AERAVA"
     gene            1099..>2069
                     /locus_tag="PKX82_11975"
     CDS             1099..>2069
                     /locus_tag="PKX82_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOF53017.1"
                     /translation="MLQILYVTFPFFALVLCGYLAARRGMLPLAAIPGLNGFVLFFAL
                     PCMLYRFGASTPIAQLLDASVALTYLLCALIMVAFVVAVTLHGRIGWNDAAFGALVGA
                     FPNTGFMGVPLLVALLGAKAAGPAIVTILVDMVVTSSLCIALSRLGETGGVSAGQAAK
                     NALKGVAANPMPWSIMLGGLVSAMQIELPKPVATTVGMLADAASPVALFTIGAVLARS
                     QIMARDDKSHEVHWQEYVPVALIKLFLHPLLVLLVGAAAMSLGVQIDKFALMVLVLLA
                     ALPSASNVSLLAERFGADNGRIARIILVSTAAAFFTFSGAVALMTGK"
BASE COUNT          314 a          627 c          677 g          451 t
ORIGIN      
        1 gggccacggc gcgctcggct tcatgctcct cgttgtcggc atcgaccacg cgcactggtt
       61 cgccttcgcc cagttcactg aatagtgtct tggggaacag cttggggttg gggccgatca
      121 cattgttggc cgcgcgcagg atggcgctgg tagagcggta gttttgctcc agcttgacca
      181 gcttgagcgt ggggaaatcg atgggcagtt ttttcaggtt gtccagcgtg gcaccgcgcc
      241 agccgtagat ggattggtcg tcatcgccca ccgccgtaaa tcgcgcgctg ctggccggat
      301 tgccaccggc cagcagcttg agcatttcgt actgcgtggc attggtgtcc tggtactcgt
      361 ctacaaggat atggcccatc agcccctgcc acttctgcct cacctgctca tgattttgta
      421 gcaacttgag tggcaggctg atcaggtcgt caaagtccac gctctggtag gcggtcagtc
      481 gctcttcgta gcggcccatg acggtagcga tgatgcgttc gttgtcgtcc gcagccagcg
      541 ccagggcctg cgccgaactc aggccggcgc ttttccatcc gctgatggtc cattgccact
      601 gccgcgcggt ggcggcgtcc acggtgccgc cagcgtcctt gaggatgctg gtgacatcgt
      661 cggtgtccag aatggagaaa gcgggtttga gtcccagcac gctgccgtcc tggcgcagca
      721 tgcgcacgcc cagcgcgtgg aaggtgcaga tctgcacgtc cttggcagcc ttgccgatca
      781 gacccttggc ccgctcgcgc atttctgcag ccgccttgtt ggtaaaggtg atggctgcaa
      841 tgcggttcgc tggcatgccg ctctggatca ggcgggcgat cttgtgcgta atcacccgtg
      901 tcttgcccga gcctgccccg gccagcacga ggcaggggcc gtgcaggtaa ttcacggctt
      961 cctgctgggc caggttcagg ccgtgggtga cagaagcggg ggaagagggg gcagaagaca
     1021 tgcagacaag gaaacagaca agcgcgccat cttaccgggt aggtgtaacg gccctgtaaa
     1081 tgccccgaca atgtgcgggt gctgcaaatt ctttacgtca cctttccctt tttcgcgctg
     1141 gtcctgtgcg gctatctggc tgcacgccgg ggcatgctgc cactggccgc cattccgggg
     1201 ttgaacggct ttgtgctgtt ctttgccctg ccgtgcatgc tgtaccgctt tggcgcatcc
     1261 acgcccattg cgcagttgct cgacgccagc gtggcactta cctacctgct gtgtgcgctg
     1321 atcatggtgg catttgtggt ggcggtcacc ctgcacggtc gcatcggctg gaacgacgcg
     1381 gcctttggcg cgctggtagg cgcttttccc aataccggct tcatgggcgt gccgctgctg
     1441 gtggcgctgc tgggggccaa ggcagctggc ccagccatcg tcaccattct ggtggacatg
     1501 gtggtgacct cttcgctatg cattgccctg tcacgcctgg gcgaaaccgg gggcgtgtcg
     1561 gccgggcagg cagccaaaaa tgcgctcaag ggcgtggcgg ccaaccccat gccatggtcc
     1621 atcatgctgg gcgggctggt ctcggccatg cagatcgagc tgccgaagcc agtggccact
     1681 accgtgggaa tgctggcgga tgcggcttca cccgtggcgc tgttcaccat tggcgccgtg
     1741 ctggcccgtt cccagatcat ggcgcgggat gacaaatcgc atgaggtgca ctggcaggaa
     1801 tacgtgcccg tggcactgat caagctcttc ctgcacccgt tgctggtgct gctggtgggc
     1861 gccgccgcta tgagcctggg cgtgcagatc gacaagttcg cactcatggt gctggtgctg
     1921 ctggcggcac tgcccagtgc cagcaatgtg tcgctgctgg ccgagcgctt tggcgcggac
     1981 aacggccgca ttgcccgcat cattctggtg tcgacggcag ccgccttctt caccttctcg
     2041 ggcgccgtgg cgctgatgac cggaaagta
//
LOCUS       DAOSJM010000409         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_47737_length_2034_cov_3.397676, whole genome shotgun sequence.
ACCESSION   DAOSJM010000409 DAOSJM010000000
VERSION     DAOSJM010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47737_length_2034_cov_3.397676"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PKX82_11980"
     CDS             <1..435
                     /locus_tag="PKX82_11980"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594540.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HOF53018.1"
                     /translation="LRGFKDTSGVMAEDVAKRLMDYGFHAPTLSFPVPNTLMVEPTES
                     ETLAELDRFIDAMIAIRGEVSKVEKGEWPQDNNPLKHAPHTAASLLGAEWDRPYSRET
                     GAFPVASLKAVKYWPSVGRVDNVYGDRNLFCSCVPVADYANT"
     gene            515..1438
                     /locus_tag="PKX82_11985"
     CDS             515..1438
                     /locus_tag="PKX82_11985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF53019.1"
                     /translation="MSAAIAASVLLAGILALTLLLGQQARLESGVQQLVDAASSARVP
                     EASKEAEQLRTSIGEARAMLLWVAAAAAVGLLAVSAAIYRLLVRPVVRLANTLRDSSG
                     QPAPHGKEPLRELQMLQDATLALEETRKELDAVRDALAQQVHTDALTGLANGRMFEMQ
                     GQQAFFHAQRYGEALSVIVLDIDHFQRINDRYGHDSGDVVLRALGRYLKEAVRAAERP
                     AARIGGEEFAILVSHTAADEPALFAERLRRGIAVMEVVMPTGSLLRLTTSAGFASRNE
                     TDQDLNTLMRRAGDALQLAKQNGRNRIERAP"
     gene            complement(1500..>2034)
                     /locus_tag="PKX82_11990"
     CDS             complement(1500..>2034)
                     /locus_tag="PKX82_11990"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOF53020.1"
                     /translation="SHPALAQEVIALLGAGGISAQADANRPFDHGTWVPLMHLLPQAN
                     VPIVQVALPVGWNARQVYAMGQTLQSLRSQGVLVMGSGSMTHNLAEFFGGEREVAPYV
                     TEFSRWIESKLAAGDLEALLNYRSQAPHAHRAHPTEDHFLPLFFALGAAGADARPEYL
                     SREVMYSMLAMDTFTLQ"
BASE COUNT          392 a          660 c          638 g          344 t
ORIGIN      
        1 ctgcgtggct tcaaggacac gagcggcgtg atggccgaag acgtggccaa gcgcctgatg
       61 gactacggtt tccacgcgcc caccctgtcc ttcccggtgc ccaacacgct gatggtggag
      121 cctaccgaga gcgaaaccct ggccgagctg gaccgtttta tcgacgcgat gattgccatc
      181 cgtggcgaag tgagcaaggt tgaaaagggc gagtggccac aggacaacaa cccgctcaaa
      241 cacgcccccc acaccgccgc cagcctgctc ggtgccgaat gggaccgccc ctacagccgc
      301 gagacaggcg ccttcccggt ggccagcctc aaggccgtga agtactggcc cagcgtgggt
      361 cgcgtggaca acgtctatgg ggaccgcaac ctgttctgca gttgcgtgcc ggtagcggac
      421 tacgccaaca cctgagccaa gcgcatctga accggcctat gattcgtgca tgtccgcacg
      481 acacaggtct ccacacaagc cagggttact ctgggtgtct gctgccatcg ccgcatcggt
      541 gctgctggca ggcatcctgg ctttgaccct tctgttggga caacaggcac gcctggagtc
      601 cggcgttcag cagctggttg acgccgcttc aagcgccagg gtgccggaag ccagcaagga
      661 agccgagcag ctacgcacat cgatcgggga agcacgcgcg atgctgctct gggtggccgc
      721 agctgctgcg gtgggtctgc tggcggtgtc tgcagccatc tatcgcttgc tggtccgtcc
      781 cgtcgtccgt ctggcaaaca cccttcgcga cagcagcgga caacctgcac cgcacggaaa
      841 agaacccctt cgggaactgc agatgctgca ggacgcgacc ctcgcgctgg aggaaacccg
      901 caaggagctc gatgccgttc gggacgcgct ggcgcaacag gtgcacaccg atgcactcac
      961 cggcctggcc aacgggcgca tgtttgaaat gcaggggcaa caggcgttct tccatgccca
     1021 gcgctatgga gaagctctgt ccgtcatcgt gctggacatc gaccacttcc agcgcatcaa
     1081 cgaccgctat ggacacgaca gtggtgatgt ggtcctgcgg gcgctggggc gctacctgaa
     1141 agaagcggtg cgtgccgcag agcgccccgc agcacgcatt ggcggcgagg aatttgcgat
     1201 cctggtttcc cataccgcag ccgacgaacc agccctgttt gccgaacgcc tgcgccgggg
     1261 cattgcggtg atggaagtgg tcatgccgac aggctcgctg ttgcgcctga ccaccagcgc
     1321 agggtttgcc agccgcaacg agaccgacca ggacctcaac accctgatgc gccgcgcggg
     1381 ggatgcgctc cagctggcca agcagaacgg gcgcaaccgg atagagcggg cgccctgatc
     1441 agggatgcta tatttttgat agctattcgc gcatattccg cgggggctat aggccaattt
     1501 cactgcaaag tgaaggtatc catcgccagc atgctgtaca tcacctcgcg gctcaggtac
     1561 tcgggccggg cgtctgcacc tgctgcgccc aatgcaaaga acagcggcag aaagtggtct
     1621 tccgtcgggt gcgcacggtg ggcatgcggc gcctggctgc ggtagttcag caaggcctcc
     1681 agatcacctg cggccagctt cgattcaatc cagcggctga attccgtcac atacggcgca
     1741 acctcccgct caccgccaaa gaactcggcc aggttgtgcg tcatgctgcc ggagcccatc
     1801 accagcacac cctggctgcg caggctttgc agggtctggc ccatggcata gacctggcgc
     1861 gcattccagc ccacaggcag cgccacctgc acaatgggga cgttggcttg gggcagcaaa
     1921 tgcatcagcg gtacccaggt gccgtggtcg aaaggccggt tggcatcggc ctgcgcggaa
     1981 atgcccccgg cgcccagcag tgcaatcacc tcctgcgcca gcgccggatg gctg
//
LOCUS       DAOSJM010000410         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_48488_length_2010_cov_3.622506, whole genome shotgun sequence.
ACCESSION   DAOSJM010000410 DAOSJM010000000
VERSION     DAOSJM010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48488_length_2010_cov_3.622506"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PKX82_11995"
     CDS             <1..174
                     /locus_tag="PKX82_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOF53021.1"
                     /translation="LEVEQKLLKRIPAKYLVDAHHWLILHGRYVCKARNPQCWQCSVA
                     GVCGFTLKATAPG"
     gene            204..1016
                     /locus_tag="PKX82_12000"
     CDS             204..1016
                     /locus_tag="PKX82_12000"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOF53022.1"
                     /translation="MADISEFLHAVKLPVMPEVAHALIRTLNDEDADVSTVRDVIAKD
                     PALTATLLRMANSAIFGLSRSVNTLDNAVSVVGMSQIRARALSICLANVFVLPQGLNR
                     LEFWRNSMACAGYAHWLAHALHMDEQQAWLTGMMQRLGEITLVQNHPAQAEVIERKPC
                     GPGERWQREREAFGFDEGQITAEIARRWDFPEDVVQALRVSASPMESAPLSRLGAVVH
                     LAGLLVDMPDVTPQTLTQLPAAVVKALGLPLDALADSIPDLDAISDTSSLAG"
     gene            complement(1013..1561)
                     /locus_tag="PKX82_12005"
     CDS             complement(1013..1561)
                     /locus_tag="PKX82_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOF53023.1"
                     /translation="MTLWLVRHAQPLVAPGVCYGALDVPADADATAKAAQALAAELPQ
                     NLFVQVSPLQRCEQLAQCLRGLRPDLAFKIDLRLQEMDFGVWEGVAWGEIPRAAMEAW
                     TADFGNHRFGDAESANGVLQRVAQVWDACCAAGQPAVWITHAGVIRAVNLIAQNQRAV
                     MNAEDWPREAPAFGAWQTLALD"
     gene            complement(1558..>2010)
                     /locus_tag="PKX82_12010"
     CDS             complement(1558..>2010)
                     /locus_tag="PKX82_12010"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOF53024.1"
                     /translation="AIAVVLALLLKVVLLAQLGSLGVAVVCTSVFLAHVVSRTWPLLM
                     IRLLPHVGDAAGSKSKPLADQITVGALLAAFFWCFLALAPVWIAYPAIKGVAIGVALA
                     FTAVAFGVMLRWFRRRLGGFTGDCLGATQQVCELAFYLGMAVGWGLSA"
BASE COUNT          401 a          696 c          595 g          318 t
ORIGIN      
        1 ctggaggtgg agcagaaact gctcaagcgc attccggcca agtacctggt ggatgcccac
       61 cactggctga tcctgcacgg gcgttatgtg tgcaaggcgc gcaacccgca gtgctggcaa
      121 tgtagcgtgg caggggtttg cgggtttaca ctcaaagcca ctgcgccggg ctgaccatag
      181 cctggccaaa aaactttttt tatatggccg acatttccga atttctgcat gctgtcaagc
      241 tgcccgtcat gcccgaagtg gcgcatgcgc tgattcgcac gctcaatgac gaagacgccg
      301 acgtctccac cgtgcgcgat gtcatcgcca aggaccccgc cctgaccgcc accctgctgc
      361 gcatggccaa tagcgccatc tttggcctgt cccgcagtgt caacacgctg gacaacgccg
      421 tgagcgtggt gggcatgtca caaatccggg cgcgcgcgct gtccatctgt ctggccaatg
      481 tgtttgtgct gccacaaggc ctgaaccggc tggagttctg gcgcaacagc atggcgtgcg
      541 ccggctacgc ccattggctg gcccatgcgc tgcatatgga cgaacagcag gcctggctga
      601 ccggcatgat gcaacggctg ggcgagatca ccttggtaca gaaccatccc gcgcaggcag
      661 aagtcatcga gaggaagccc tgcggccccg gtgaacgctg gcagcgcgag cgcgaagcct
      721 tcggtttcga cgagggccag attacggccg agattgcccg gcgctgggat tttccggaag
      781 acgtggtgca ggccctgcgg gtcagcgcaa gccctatgga atcggcccca ctgtcccgcc
      841 tgggcgcggt ggtgcacctg gccggcctgc tggtcgatat gccggacgtc acgccgcaaa
      901 cactgaccca actgcctgcc gctgtggtga aggccctggg gctgccactg gacgcactgg
      961 ccgactcgat tcccgacctg gacgccatca gcgacacgtc ctcgctggca ggctaatcca
     1021 gcgccagggt ctgccaggcg ccaaatgcag gcgcctcgcg cggccagtct tcggcgttca
     1081 tcactgcacg ctggttttgc gcaatcagat tcactgcacg gatcaccccc gcatgcgtga
     1141 tccagacggc aggctggccg gcagcgcagc acgcatccca cacctgcgcc acgcgctgca
     1201 gcaccccatt ggcgctctcg gcgtcgccaa agcgatgatt cccgaagtcg gccgtccagg
     1261 cctccatcgc agcgcgggga atctcgcccc aggccacgcc ttcccacacg ccaaagtcca
     1321 tttcctgcag gcgaagatcg attttaaagg ccaaatcggg ccgcagcccc cgcaaacact
     1381 gcgcaagctg ctcacatctt tgtagcggag acacctgcac gaacagattc tgtggcaact
     1441 ctgccgccag ggcctgcgca gctttcgccg tggcgtctgc atctgcaggt acgtccagcg
     1501 ccccgtagca cacgccgggc gccaccaggg gctgcgcatg acgcaccagc cacagggtca
     1561 tgcggacagc ccccagccca cggccatgcc aaggtaaaac gccagctcgc acacctgctg
     1621 ggtggcgccc aggcagtccc cggtaaagcc gcccaggcgg cgccggaacc agcgcagcat
     1681 gaccccaaac gccacggccg tgaacgccag tgccacgcca attgctacac ctttgatagc
     1741 tgggtacgca atccatacgg gggccagcgc cagaaaacac caaaaaaatg cagccagcaa
     1801 cgctcccaca gtgatctggt cggccagcgg cttggatttg gaaccggccg cgtcgcccac
     1861 atgcggcagc aggcgaatca tcagcagcgg ccaggtacgt gacaccacgt gggccaggaa
     1921 caccgacgta cacaccaccg ccacgcccag cgagcccagt tgcgccagca acaccacctt
     1981 gagcagcagc gccagcacca ccgcaatggc
//
LOCUS       DAOSJM010000411         2006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_48613_length_2006_cov_5.706304, whole genome shotgun sequence.
ACCESSION   DAOSJM010000411 DAOSJM010000000
VERSION     DAOSJM010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48613_length_2006_cov_5.706304"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="pntA"
                     /locus_tag="PKX82_12015"
     CDS             complement(<1..713)
                     /gene="pntA"
                     /locus_tag="PKX82_12015"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520899.1"
                     /note="forms a tetramer composed of 2 alpha subunits and 2
                     beta subunits in the inner membrane; involved in
                     catalyzing transfer of hydride ion equivalents between NAD
                     and NADP; stereospecific (AB-specific); functions as a
                     proton pump by translocating protons from cytoplasm to
                     periplasm; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HOF53025.1"
                     /translation="MQSGPSTAVPQRIGVPREIFPGEKRVATVPEAVQKLIKLGFAVS
                     VESGAGVLANFDDESFRDAGADVLPDAVSLWAQADIVFKVRAPTSDEIAMMRPGTTLV
                     SFIWPAQNPEMLQQLAARQVTVLAIDALPRTLSRAQKMDALTSMAGVSGYRAVIEAAN
                     AFGRFFNGQITAAGKVPPAKVFIAGAGVAGLSAIGTAANLGAIVRANDTRAEVADQVV
                     SLGGEFVKVDYEEEGSGGGG"
     gene            895..1404
                     /locus_tag="PKX82_12020"
     CDS             895..1404
                     /locus_tag="PKX82_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOF53026.1"
                     /translation="MNERWKPSTTVAAVIENDGRFLLVEEHTPEGLRLNNPAGHLEPG
                     ESLVQGCEREVLEETAHAFRAQALVGIYLSRFQRPANLQNGTPLQDITYLRFAFCGQL
                     GLFDAQRRLDTGIVRTVWLSADEIRQSATRHRSAMVLRCMEDYLRGQRYPLDVLTTDA
                     SALVWPISS"
     gene            1494..>2006
                     /locus_tag="PKX82_12025"
     CDS             1494..>2006
                     /locus_tag="PKX82_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOF53027.1"
                     /translation="MRKQRIVVGLSGGVDSAVTAYLLKQQGHEVIGIFMKNWEDDDDS
                     EYCSSNIDFVDAAAVADVLGIEIEHVNFAANYKDRVFSEFLREYQAGRTPNPDILCNA
                     EIKFKAFLDHAMRLGAENIATGHYARVRHNLSTGLFELLKGLDASKDQSYFLHRLNQA
                     QLSKTLFPVGE"
BASE COUNT          420 a          628 c          572 g          386 t
ORIGIN      
        1 ccgccaccgc ccgagccttc ttcctcgtag tcgaccttga cgaactcgcc gcccaaggag
       61 acgacctggt cggccacctc ggcacgcgtg tcattggcgc gcacgatggc gcccagattg
      121 gccgcggtgc cgatggccga gagaccggct acacccgcgc cggcaataaa gactttggcc
      181 ggcggaacct tgcccgccgc cgtaatctgg ccgttgaaga agcggccaaa ggcgttggcc
      241 gcctcgatga cagcgcggta gccgctaacg ccggccatgg aggtcagggc gtccatcttc
      301 tgggcccggc tcagggtacg cggcagcgcg tcaatggcca gcacggtgac ctgcctggcg
      361 gccagttgct gcagcatttc cgggttttgg gcaggccaga tgaagctgac gagggtcgtt
      421 ccaggccgca tcatcgcgat ctcgtccgac gtcggggcac gcaccttgaa aacgatatcg
      481 gcttgcgccc acagggacac cgcatccggc agcacgtccg cacccgcatc gcggaaactc
      541 tcatcgtcaa aattggccag cacccccgca ccggactcga cagagacggc aaatcccagt
      601 ttgatcagtt tctgtactgc ttcgggcacg gtggccacgc gcttttcgcc tgggaaaatt
      661 tccctgggca caccgatacg ctgcggtact gcagtggatg gtcccgattg catattgcga
      721 ctcctgatat cgaactgtgt gtggaaatgc gccactgcct gccactcggt aagcttggcg
      781 aaaggattgc cgactcaact gtaactgaat ttggtgcctc aacagtcact tagatgcctc
      841 tccccccact ggcggactgg cagacagcgc cttgtttcca gcacataatt ccccatgaac
      901 gaacgttgga aacccagcac caccgtagca gccgtcatcg agaacgatgg ccggtttctg
      961 ctggtggaag agcacacccc tgaaggcctg cggctgaaca acccggccgg ccatctggaa
     1021 cccggcgaat ccctggtgca agggtgcgag cgcgaagtcc tagaggaaac ggcccatgca
     1081 tttcgggccc aggccctggt gggcatctac ctgtcgcgat ttcagcgccc tgccaacctg
     1141 cagaacggta ccccgctgca ggacatcacc tacctgcgtt tcgctttttg cggtcagctc
     1201 ggcttgttcg acgcccagcg ccgtctggac accggcatcg tgcgcacggt ctggctcagc
     1261 gccgacgaga ttcgccagag cgcgacccgt caccgcagcg ccatggtgtt gcgctgcatg
     1321 gaggattacc tgcggggcca gcgctacccg ctggatgtgc tgaccaccga cgccagtgca
     1381 ttggtgtggc caattagctc ctgaatttat agctatatcc gcagcactga cgggggctaa
     1441 aggtcaattt cattcaatat tcagaaatac tgcaccccgg taaaatggca ggcatgcgca
     1501 aacaaagaat cgtcgtcgga ctgagtggag gtgtggactc cgccgtaaca gcctatctgc
     1561 tgaagcagca ggggcacgag gtcatcggca tcttcatgaa gaactgggaa gacgacgatg
     1621 acagcgagta ttgctcctcc aacatcgact ttgtcgatgc cgctgcggtg gccgacgtgc
     1681 tgggcatcga gattgaacac gtcaactttg cagccaacta caaagaccgc gtgttttccg
     1741 aattcctccg cgaataccag gctggacgca cccccaatcc ggacatcctg tgcaacgcag
     1801 aaatcaaatt caaggcgttt ctggatcatg ccatgcgtct cggggctgaa aacatcgcca
     1861 caggtcacta tgcccgtgtc cgacacaacc tgtccaccgg actgtttgaa ctgctgaaag
     1921 gtctggatgc gtccaaagac cagagctact ttttgcaccg gttgaatcag gcgcaactgt
     1981 ccaaaacgct gttcccggtc ggcgaa
//
LOCUS       DAOSJM010000412         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_48680_length_2004_cov_3.859928, whole genome shotgun sequence.
ACCESSION   DAOSJM010000412 DAOSJM010000000
VERSION     DAOSJM010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48680_length_2004_cov_3.859928"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2004
                     /locus_tag="PKX82_12030"
     CDS             <1..>2004
                     /locus_tag="PKX82_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOF53028.1"
                     /translation="DHVAKSSTAAHQSDHIFKACGTPVFFPANVQEILDLGIHAFAMS
                     RFSGVWAGMKTIQEIVESSATAMIDPDRVQIKIPTDFPMPPGGVHIRWPDAALDQEAR
                     LFDTKWYAALAYIRANRLNYNVIEGKNDRFGLIASGKAFNDTRQALADLGLDDATCQR
                     LGIRLHKVGVVWPLEAQLTREFATGLQEILVVEEKRQVIEYQLKEELYNWRPDVRPTV
                     LGKFNEVEGEDRYGAGGEWSMPNPTANTLLRANADLSPAIIARAIAQRLKKLGIDADT
                     TARIDAQLAVLSAKESSMQVLEVSTSAAIRQPWFCSGCPHNTSTQVPEGSRAMAGIGC
                     HFMSIWMDRSTTGFTQMGGEGVPWVGQQPFSHDQHMFANLGDGTYFHSGLLAVRQSIA
                     AGVNITYKILYNDAVAMTGGQQVGERPEGHSVVQIAQSMRAEGAIKITIVTDEPEKYV
                     GVKGIPDGISIQHRDALDAVQREFREIKGTTVIIYDQTCATEKRRRRKRGTLVDPAKR
                     VVINELVCEGCGDCSVQSNCISVEPLETELGRKRQINQSTCNKDLSCVKGFCPSFVTV
                     EGGSLKKKTKSSGNSAEGNWPALPMPEVADVHTAPGGVWGIVVAGVGGTGVITIGQLL
                     GVAAHLEGKGIVTQDAGGLAQKGGATWSHVLIGAMQDAIRTTR"
BASE COUNT          417 a          604 c          631 g          352 t
ORIGIN      
        1 acgaccacgt ggccaagagc tccacggcag cgcaccagag tgaccatatt ttcaaggcct
       61 gcggcacgcc cgtgttcttt ccagccaatg tgcaggaaat tctggacctg ggcatccatg
      121 cgtttgccat gagccgcttc tccggcgtgt gggccggcat gaaaacgatc caggaaatcg
      181 tggaatccag tgccaccgcc atgatcgatc cggaccgggt gcaaatcaag attccgacgg
      241 actttcccat gcctcccggt ggcgtgcaca tccgctggcc agatgctgcg ctggaccagg
      301 aagcgcgtct gtttgacacc aagtggtacg ccgcactggc ctacatccgc gccaaccgcc
      361 tgaattacaa cgtcatcgaa ggcaagaatg atcgattcgg tttgatcgcc agtggcaagg
      421 cgtttaacga cacgcgtcag gctctggcgg atctggggct ggacgatgcc acctgccagc
      481 gcctgggtat ccgcctgcac aaggtcggtg tggtgtggcc gctggaagcg cagctcacgc
      541 gcgaatttgc caccgggctg caggaaatcc tggtggtcga ggaaaaacgc caggtcatcg
      601 agtaccagct caaggaagag ttgtacaact ggcgccctga tgtgcgcccc accgtgttgg
      661 gcaagttcaa cgaagtggaa ggcgaagacc gctatggcgc cggtggcgag tggtccatgc
      721 ccaaccccac ggccaacacc ctgctgcgtg cgaacgccga tctgtcacct gccatcattg
      781 cccgtgcgat tgcccagcgt ctcaagaagc tgggcatcga tgcggacacc accgcccgca
      841 ttgatgcgca actggccgtc ctgagcgcca aggaaagctc catgcaggtg ctggaggtca
      901 gcacaagtgc tgcgattcgc cagccctggt tttgttccgg ttgcccgcac aacaccagta
      961 cccaagtgcc cgagggttcg cgtgccatgg ccggcattgg ctgccacttc atgagcatct
     1021 ggatggaccg ctccaccacg ggcttcaccc agatgggggg cgagggtgtg ccgtgggtcg
     1081 ggcagcaacc cttcagccat gaccagcaca tgtttgcgaa cctgggcgat ggcacctact
     1141 ttcatagtgg tctgctggcc gtgcgccaga gcattgcagc gggcgtcaac atcacctaca
     1201 agattctcta caacgacgcc gtggctatga ccggcggcca gcaggtgggc gaacggcccg
     1261 aaggccattc ggtggtgcag atcgcgcaga gcatgcgtgc agaaggcgcc atcaagatca
     1321 ccatcgtcac cgacgagccc gaaaagtacg tcggagtgaa gggtattccc gatggcattt
     1381 ccatccagca ccgcgatgcg ctggacgcgg tgcagcgaga gttccgtgag atcaagggca
     1441 ccaccgtcat catctacgac cagacctgcg ccaccgaaaa gcgccgccgc cgcaagcgtg
     1501 gcaccctggt ggatccggcc aaacgcgtgg tgatcaacga gctggtgtgc gagggctgcg
     1561 gcgactgcag tgtgcagagc aactgcatca gcgtggagcc actggagacc gaactcggcc
     1621 gcaagcggca gatcaaccag agcacctgca acaaggacct gagttgcgtg aaaggcttct
     1681 gtccgagctt tgtgacggtg gaaggcggtt cgctcaagaa aaaaaccaag tcttccggca
     1741 atagcgccga aggaaattgg ccagcactcc ctatgcctga ggtggccgat gtgcacaccg
     1801 cgccaggggg cgtctggggt attgtggtgg cgggcgtcgg cggcactggc gtgatcacca
     1861 ttggtcagtt gctgggtgtg gcagcccacc tggaaggcaa gggtatcgtc acccaggacg
     1921 ctggtggact ggcccaaaag ggcggcgcta cctggagcca tgtgctcatt ggggccatgc
     1981 aggacgccat tcgcaccacg cgtg
//
LOCUS       DAOSJM010000413         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_48740_length_2002_cov_4.455059, whole genome shotgun sequence.
ACCESSION   DAOSJM010000413 DAOSJM010000000
VERSION     DAOSJM010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48740_length_2002_cov_4.455059"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..428
                     /locus_tag="PKX82_12035"
     CDS             18..428
                     /locus_tag="PKX82_12035"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOF53029.1"
                     /translation="MSTNASPQSVQLPTDEELVLKVIPMPADCNANGDIFGGWVMAQV
                     DLAGSVVPARYTQGRMATVAVNEFIFKQPVRVGDILSFFSKVSRIGRTSITVKVEVYA
                     ERFRTQGQYIKVTEATLTYVAIDDNGKPREIPKV"
     gene            complement(668..1081)
                     /locus_tag="PKX82_12040"
     CDS             complement(668..1081)
                     /locus_tag="PKX82_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF53030.1"
                     /translation="MLLTDIAVEHTLVSKNNGVRQTYLLHPFTNTQRDTLGKFEIVRD
                     IREPGFKDVKRSAFVTFQQLAELYAKGVLEEFGFSVRMCPGQGTSPTVNPAKKILPTS
                     IRAGSSFDLAVQKVDVSKPANRELRTALLRTNVKL"
     gene            complement(1169..1285)
                     /locus_tag="PKX82_12045"
                     /pseudo
     CDS             complement(1169..1285)
                     /locus_tag="PKX82_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1525..>2002
                     /locus_tag="PKX82_12050"
     CDS             1525..>2002
                     /locus_tag="PKX82_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HOF53031.1"
                     /translation="MKTAFPLFAGQCVSAMARLAAGMLLGTAMGAVAAAPKLPYSYFS
                     VGDPAITPVISPSDKPATPSYVIMGGGPDVDTAFQWMIQRAGIKPGTGGRFVIIRATG
                     TEAYNPYIYYSDANGGTSTLPPTNQDGWVGGAYLGLSSVETLVIPSVTAANHPTVNQ"
BASE COUNT          434 a          586 c          553 g          429 t
ORIGIN      
        1 acccaaggat tgtgcccatg tctacgaatg caagccccca gagcgtccaa ttgcccaccg
       61 acgaagagct ggtgctcaag gtcatcccca tgccggcgga ctgcaacgcc aatggcgata
      121 tctttggtgg ctgggtcatg gcgcaggtgg acctggccgg ttcggtcgta ccggcgcgct
      181 acacgcaggg ccgcatggcc acggtggcag tgaacgaatt catcttcaag cagccggtgc
      241 gcgtgggcga catcctgagc ttcttctcca aggtcagccg catcggccgc acctccatca
      301 cagtgaaggt ggaggtgtat gccgagcgct ttcgcaccca gggccagtac atcaaggtga
      361 ccgaggccac cctgacctac gtggccatcg acgacaacgg caagccgcgg gaaattccga
      421 aggtttgagg gcagcaggct gggccgcgct caatccctga ggctcacccc cagggtatgc
      481 agcttcttgg catacgagtc cctgcgtttt gcggcctttg cagctgtttc ggtggccgct
      541 tgaactgctt tgggcccctt ttgcgcagca gctttgatca atccgaactt gtgcgcagca
      601 tctgcctgcg cccgcttgaa agtggcgatg agctcggcat ggacaacgga gggattcaca
      661 ggcaccctta cagtttgacg ttagtgcgaa gcaatgctgt tcttagttcg cgattcgcag
      721 gttttgaaac atccactttt tgaaccgcca ggtcgaacga cgagccagcc ctgatgctgg
      781 tgggcagtat cttctttgca ggattcacag tgggggacgt accctgaccc ggacacatgc
      841 gaacggaaaa cccgaattct tcgagtacgc ctttcgcata caactccgcc aattgttgaa
      901 acgtcacgaa ggctgagcgt ttaacatctt tgaaccctgg ctcacggatg tcacgaacga
      961 tctcgaattt accaagcgta tcgcgttgcg tattggtaaa cggatgcagc agataagtct
     1021 gacgaacccc attatttttg gacaccagcg tgtgctcgac ggcgatatca gttaagagca
     1081 ttttgttttc caatgtattc aagccacagc tcgcccagcc gcatcagccg ccatcccccg
     1141 atttccactg cacctaaaag tcgaacagtt atgcagccat ggccagatgc tgcttcgggg
     1201 tgaaaccgcc cagagccata ttcgggcggt cgtgattgta ggaccacatc cattgcgtgg
     1261 cgaagtcctg aacttcttca atactggacc agtcctactg ggatatccgt ctgccgcatc
     1321 catacttgat cttcaggagc aatcagctat tcgttcatac ggaacgggag gtgcagagaa
     1381 ctgtgtgcaa tagttcaaac aggctaattt ccagatgaaa gaccgaggct aaacgtcctt
     1441 aaaaagtcgt tttatttgat cctgcacccc ttgcaaaata ttcccaaagc gcatactgga
     1501 ggagttatct gaaaggagct tggtatgaag acagcgtttc ccctctttgc aggccagtgc
     1561 gtttctgcaa tggccagatt ggcagccggg atgttgctcg gcacggcgat gggggccgtc
     1621 gcggcggcgc ccaagcttcc ctacagctat ttttcagtcg gtgatccggc gatcacaccg
     1681 gtcatcagcc cgtcggacaa gcctgcgaca ccgtcctacg tgatcatggg cggtggcccg
     1741 gacgttgaca cggccttcca gtggatgatc cagcgtgcgg gtatcaaacc gggcactggc
     1801 ggccgcttcg tgatcatccg cgcaaccggc acggaagcct acaaccccta catctactac
     1861 agcgacgcca atggcggcac gtccaccctg ccgcccacca accaggacgg ctgggtgggc
     1921 ggtgcctacc tgggactgag ctcggtggag acactggtga tccccagcgt cactgcagcc
     1981 aaccacccta cggtgaacca ga
//
LOCUS       DAOSJM010000414         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_48851_length_1999_cov_3.482510, whole genome shotgun sequence.
ACCESSION   DAOSJM010000414 DAOSJM010000000
VERSION     DAOSJM010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48851_length_1999_cov_3.482510"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="PKX82_12055"
     CDS             <1..385
                     /locus_tag="PKX82_12055"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOF53032.1"
                     /translation="QRCSPLCRRGRCSHHLCPVIGQVWIGIMPNEHTNVIGLSKYARL
                     AEWIMGRPQIQEEAVVQLADMIQERTQPDGLAIVMEASHFCMSWRGVKDMDSKMINSV
                     MRGVFLKDPNLRREFLSLIPKKTNS"
     gene            414..836
                     /locus_tag="PKX82_12060"
     CDS             414..836
                     /locus_tag="PKX82_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOF53033.1"
                     /translation="MLVRLLYASRAVDCSDAAIDAILSQSRQSNPTCGITGILCYGGG
                     IFLQAIEGGRMAVSELYGHIQRDKRHKDVVLLHYEEISERRFGGWTMGQVNTSRINTS
                     ILLKYAEKPELDPYAVSGRVSLALLEELMATASIIGRA"
     gene            852..1700
                     /locus_tag="PKX82_12065"
     CDS             852..1700
                     /locus_tag="PKX82_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HOF53034.1"
                     /translation="MQPLVGCDFSSAPSRRKQIALAWGLLEQGRVRVTEIQRLDSLDA
                     FSAWLHEPRAWVGGFDLPFGLPRELVETLGWPLAWEPCMRHYASLDRAQIRNCFAAFC
                     AARPAGRKFAHRATDIPAGSSPSMKWVNPPVAYMLHAGVPRLLEAGVYLPGLQPAVQA
                     METTPERAAKVALEAYPGLLAREILGHRSYKTDDRARQSDERLIARKDLVTALELGQT
                     RLQLRLKLTHAQRDAIIEDGSGDALDAVLCLMQAAWAWQQHCQGAPLYGLPADMDPLE
                     GWIVTA"
     gene            complement(1712..>1999)
                     /locus_tag="PKX82_12070"
     CDS             complement(1712..>1999)
                     /locus_tag="PKX82_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOF53035.1"
                     /translation="NQQKRQNVRMVINQTARLGDGRAITMQLQQVLDRFVVSEPGKPV
                     RLIHMGDIPADPSVRQAIMRRQLLMQSMPGCPAAMAVTQVAIKLQEALLPR"
BASE COUNT          360 a          604 c          629 g          406 t
ORIGIN      
        1 gcaaaggtgc tctcccctct gccgccgtgg ccgctgcagc caccatcttt gcccggtgat
       61 tggccaggtg tggatcggga tcatgcccaa cgagcacacc aatgtcattg ggctctccaa
      121 atacgcacgt ctggcggagt ggatcatggg ccgcccgcag atccaggaag aagcggttgt
      181 gcaactggct gacatgattc aggagcggac ccagcccgat ggtctggcca tcgtcatgga
      241 agccagccat ttctgcatgt cctggcgcgg cgtgaaggac atggacagca agatgatcaa
      301 ttcggtcatg cgcggcgtgt tcctcaagga ccccaacctg cgccgggaat tcctgtcgct
      361 gatccccaag aaaaccaatt cctgactccc caatttccca cgaaaggtct gccatgcttg
      421 tacgtctgct ctatgccagc cgcgccgtag actgcagtga cgcagctatc gacgccattc
      481 tgagccagtc ccgccagtcg aatccgacct gcggcatcac cggcatcctc tgctacggcg
      541 gtggtatttt tttgcaggct atcgagggcg ggcgcatggc tgtcagcgaa ctgtacggac
      601 atatccagcg tgacaagcgc cacaaggacg tggtgctgct gcactacgaa gaaatctctg
      661 aacgccgctt tggcggctgg accatgggcc aggtcaatac ctcgcgcatc aataccagca
      721 tcctgctgaa gtatgccgag aagcccgaac tggacccgta tgccgtgtct ggcagggtct
      781 ctctggccct gctcgaagag ttgatggcca ccgcgtccat catcggccgc gcctgatccg
      841 ggcgtagcgc attgcaaccg ctggtaggct gcgatttttc cagcgcgcct tccaggcgca
      901 aacagattgc actggcctgg gggctgctgg agcaaggccg ggtccgcgtc accgagattc
      961 agcggctgga ttcgctggac gcattcagtg cctggttgca tgagccgcgt gcctgggtag
     1021 gtgggtttga cctgcctttt ggcctgccac gcgaactggt ggagaccctg ggctggcccc
     1081 ttgcttggga gccttgcatg cgccactatg catcgctgga cagggcgcag atacgcaact
     1141 gttttgccgc gttttgtgct gcgcgtcccg ctggtcgcaa attcgcccat cgggcgactg
     1201 atattcctgc cggttccagt ccctcgatga aatgggtcaa tccgccggtg gcttacatgc
     1261 tgcatgcggg cgtgccacgc ttgctggagg cgggggtgta tttgccaggc ttgcagccgg
     1321 cagttcaggc aatggaaaca acgcctgagc gcgccgcgaa agtcgctctg gaagcctatc
     1381 ccggactgtt ggcgcgcgaa atcctcgggc accgcagcta caagacggat gaccgggccc
     1441 gacaaagtga tgaacgactc atcgcgcgca aggacctggt gacggcactg gagctggggc
     1501 aaacgcggct gcagcttcgg ctcaagctga cgcacgcaca gcgcgatgcg atcatcgaag
     1561 atggttctgg cgatgcgctg gatgcggtgc tatgcctgat gcaggctgcc tgggcctggc
     1621 agcaacactg ccagggtgct ccgctctatg gtctgcctgc cgatatggat ccgctggagg
     1681 gttggattgt gacggcctga gcctgtgcaa cttagcgcgg caacagcgct tcctgcagct
     1741 tgatggctac ctgggtcacg gccatggccg ccgggcatcc gggcatggat tgcatcagca
     1801 gctgccggcg catgatggcc tggcgcaccg agggatcggc tggaatgtcg cccatgtgga
     1861 tcagccgtac cggtttgccg ggctcactga ccacaaagcg gtccagcacc tgctgcagtt
     1921 gcatggtgat ggcgcggccg tcgcccagac gcgcagtctg gttgatgacc atgcgcacgt
     1981 tttgccgctt ctgctggtt
//
LOCUS       DAOSJM010000415         1993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_49054_length_1993_cov_3.963880, whole genome shotgun sequence.
ACCESSION   DAOSJM010000415 DAOSJM010000000
VERSION     DAOSJM010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49054_length_1993_cov_3.963880"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PKX82_12075"
     CDS             complement(<1..1010)
                     /locus_tag="PKX82_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOF53036.1"
                     /translation="MTNELSAAESALREAAREYHRFPVRGKIAVSPTKPLSNQRDLSL
                     AYSPGVAYPCLDIEADPTKAAEYTSRGNLVGVVTNGTAVLGLGNIGPLASKPVMEGKG
                     CLFKKFSGIDVFDIELAENDPDKLVDIIAALEPTLGGINLEDIKAPECFYIEKKLRER
                     LNIPVFHDDQHGTAIISSAALLNGLELVGKAIGEVKLAVSGAGAAALACVDVMVGLGV
                     KRSNVFMCDSKGVIYEGRPGGYDESKARYAQKTEARTLADAVVNADVFLGCSTAGVLS
                     GDMVKTMARQPIVLALANPEPEIRPELALAARPDCIMATGRSDYPNQVNNVLCFPYIF
                     RGA"
     gene            complement(1103..1564)
                     /locus_tag="PKX82_12080"
     CDS             complement(1103..1564)
                     /locus_tag="PKX82_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOF53037.1"
                     /translation="MSSAPNDAAPSVDQVNSDYLETLLGYNARRAALSIIDVFLRKME
                     EHDFRPVDFSVASLIVHNPGITSRQLCTALNILPPNLVGMVNAMEKRGLIIRRPHPND
                     GRAMGLHPTPEGKALMAIAEEDAYKLEIAAAPRLTAAERKTLIRLLQKIYL"
     gene            complement(1570..>1993)
                     /locus_tag="PKX82_12085"
     CDS             complement(1570..>1993)
                     /locus_tag="PKX82_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOF53038.1"
                     /translation="VAMIYEGTNEIQAIDLLVRKILSDGGATFSAWLRDLRSELDDGN
                     PADQKTAARIAVLEKATMGIIAAAAADGTGAYWVADDYLRAVAVLLLDWAWLKIAREA
                     PDHSARWAAPEQALQRWVMPEFGMRIAIISAPAVALTA"
BASE COUNT          401 a          598 c          597 g          397 t
ORIGIN      
        1 gcgccacgga agatgtaggg gaagcacagc acgttgttga cctggttggg atagtccgag
       61 cgccccgtgg ccatgatgca atccggacgt gcggccaggg caagttctgg ccggatttca
      121 ggttcggggt tggccagcgc caggacgatg ggctggcgtg ccatggtttt gaccatgtcc
      181 cccgacagca ctcccgctgt ggaacaaccc aggaacacgt ctgcattgac taccgcgtct
      241 gccagtgtgc gggcctccgt tttttgggcg taacgggcct tggactcgtc atagccgcca
      301 gggcgacctt cgtagatgac gcccttggaa tcgcacatga acacgttgga gcgcttcaca
      361 cccagcccga ccatgacatc cacgcaggcc agcgcagcag cgcctgcacc ggacaccgcc
      421 agtttgactt caccaatggc tttgcccacc agttcgagac cattgagaag cgctgccgag
      481 gaaatgatgg ccgtgccatg ctggtcgtca tggaacaccg ggatattcag ccgctcacgc
      541 aacttcttct caatgtagaa gcactctggc gccttgatgt cctccaggtt gatgcccccc
      601 agcgtgggct ccagggcggc gatgatgtcc accagcttgt ccggatcgtt ctcggccagc
      661 tcaatgtcga acacgtcaat gccggaaaac ttcttgaaca gacagccctt gccctccatc
      721 accggcttgc tggccagggg gccgatattg ccgagtccca gcacagccgt gccattggtg
      781 accacaccga ccagattgcc acggctggtg tactcggccg ctttggtggg atcggcctca
      841 atgtccaggc agggatacgc cacaccgggc gagtacgcca gcgacaggtc gcgctggttg
      901 gacaggggct tggtgggtga tacggcgatc tttccgcgaa ccgggaaacg gtggtattca
      961 cgcgcggctt cacgcagcgc cgattcggct gcggacaatt cgttggtcat ggagggtcct
     1021 gaactggagg gttatagctt cactggccaa acggccacct gtgaaattct atgacgacga
     1081 gtccctggca gcgggcgaca cgctataggt agattttttg cagcaagcgg atcagtgtct
     1141 tgcgctcggc ggctgtcagt cttggcgcag cggcaatctc gagcttgtag gcatcctctt
     1201 cggcaatggc catcaacgct ttgccttcgg gcgtagggtg caatcccatg gcgcgcccgt
     1261 cgttgggatg cggacggcgg atgatcagtc cgcgtttttc cattgcattg accatgccca
     1321 ccaaattggg cggcaagatg ttgagcgccg tgcatagctg gcgtgacgtg atgcccgggt
     1381 tgtgcacgat cagcgaagcc accgagaaat cgaccggacg gaaatcatgc tcctccatct
     1441 tgcgcaaaaa cacatcaatg atggacagcg cggcccgccg ggcgttgtag cccaacaggg
     1501 tctcaaggta gtcagagtta acttggtcta cgctgggtgc agcatcattg ggggcagagg
     1561 acatggaggt caggcagtca gggcaacagc aggtgcgctg atgatagcga ttcgcatacc
     1621 aaactccggc ataacccaac gctgcaatgc ctgttccggc gcagcccacc gtgcgctgtg
     1681 gtcaggcgct tcgcgtgcga tcttcagcca cgcccaatcg agcaaaagta cagccacagc
     1741 ccgcaagtag tcgtcggcga cccagtaagc acccgtcccg tcagccgccg cagctgcaat
     1801 gatgcccatt gttgctttct ccagcacggc aatccgggca gcagtcttct gatcggctgg
     1861 attgccatcg tccagctcgg aacggaggtc ccgcagccat gccgagaaag ttgcaccccc
     1921 gtcagacagg atcttgcgca ccagcagatc gatcgcctga atttcgttgg taccttcgta
     1981 aatcatggca aca
//
LOCUS       DAOSJM010000416         1968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_49924_length_1968_cov_3.425510, whole genome shotgun sequence.
ACCESSION   DAOSJM010000416 DAOSJM010000000
VERSION     DAOSJM010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49924_length_1968_cov_3.425510"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PKX82_12090"
     CDS             <1..415
                     /locus_tag="PKX82_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOF53039.1"
                     /translation="ATASGLVANLAGFTIPLWLDPTVNRLGAASLALGLMAAGAGMQF
                     GALARAKTLAVSVLTIRHFLLPLVALGVSHLFGLSAVQTTVMLAFSALPTASSCYVLA
                     ARMGYDGVYVAGLVTLSTLLGMVSLPFALGVLRAF"
     gene            complement(412..1479)
                     /gene="queG"
                     /locus_tag="PKX82_12095"
     CDS             complement(412..1479)
                     /gene="queG"
                     /locus_tag="PKX82_12095"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012837346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOF53040.1"
                     /translation="MVSQIQAWARELGFSQIGVADVDLSSAEPGLLAWLSQGFHGDMA
                     YMASHGLKRARPAELVPGTVSVLTARMDYLPRTMEEGWQAVEWSRLQRPQEGIVSLYA
                     RGRDYHKVLRSRLQKLAERIAVEVGPLGFRVFTDSAPVLEAELAARSGQGWRGKHTLL
                     LNREAGSLFFLGEIYLDLPLAHSAPVTDHCGSCDACMAVCPTQAIVAPHRLDARRCIS
                     YLTIEHAGAIPLELRPLIGNRIYGCDDCQLVCPWNKFAQRSVLADFDPREGLAGQPLV
                     QLLAWTEEEFLLRTEGSPIRRIGHERWLRNVAVALGNALRTAVDAAAAQELRAALALR
                     SEHPSALVREHVQWALDVPAG"
     gene            1537..>1968
                     /gene="tsaE"
                     /locus_tag="PKX82_12100"
     CDS             1537..>1968
                     /gene="tsaE"
                     /locus_tag="PKX82_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOF53041.1"
                     /translation="MRTLLWSTEADTQVFAMRWAHSAAIGNAFVALHGDLGAGKTTLV
                     RHLLKALGVQGRVKSPTYAVVEPYELPAMNVWHFDFYRFTDPREWEDAGFRDIFASHG
                     LKLAEWPEKAAAMLPVADVDVQIRTLDDATRQVTLTAHTATG"
BASE COUNT          348 a          664 c          599 g          357 t
ORIGIN      
        1 ggcgaccgcc tcaggcctgg tagccaacct cgcaggcttc accattccgc tgtggctgga
       61 tcccaccgtc aaccggctag gggctgcatc gctggcactg ggtctgatgg ctgctggtgc
      121 cggcatgcag tttggtgcac tggcccgggc caagaccctg gcagtctcgg tgctgaccat
      181 ccggcatttc ctgctgcccc tggtggcact gggggtatcg catctgtttg ggctttctgc
      241 cgtgcaaacc acggtgatgc tggccttttc tgctctcccc acggcatcga gctgctatgt
      301 cctggcggcc cgcatggggt atgacggtgt ctacgtggcc ggactggtca cactgtccac
      361 gctgctgggc atggtgagcc tgccgtttgc gctgggcgta ctgcgcgcgt tctaacccgc
      421 tggcacatcc agtgcccact gcacatgctc ccgcaccagc gcactggggt gctccgaacg
      481 tagggctagc gcggccctga gttcctgcgc agcagcggcg tcgactgccg tccgcaacgc
      541 attgcccagg gctactgcca cattgcgcag ccagcgttca tggccaatac gccggatcgg
      601 actgccttcg gtgcgcagca ggaactcttc ttcggtccag gccaacagtt gcaccagggg
      661 ttgccccgcc agcccctcgc gcggatcaaa atctgccaag acactgcgct gcgcaaattt
      721 gttccaggga cacaccagct ggcagtcgtc gcaaccgtag atgcggttgc cgatcagggg
      781 gcgcagctct agcggaatgg cgccagcatg ctcaatcgtc aggtaggaga tgcagcgccg
      841 cgcatccagc cgatggggag ccacaatcgc ctgcgtgggg cagaccgcca tacaggcatc
      901 gcaggacccg caatggtctg tcaccggtgc actgtgggcc agcggcaggt ccagatagat
      961 ttcacccagg aaaaacagcg acccggcctc gcggttgagc agcagcgtgt gtttgccgcg
     1021 ccagccctgg ccgctgcgtg ctgccagctc ggcttcgagc accggggccg aatcggtgaa
     1081 cacacgaaac cccagcggcc cgacttcgac ggcaatcctt tccgccagct tctgcaggcg
     1141 gctgcgcagc accttgtggt agtcccgccc gcgggcatac agcgacacaa tgccctcctg
     1201 tggtcgctgc aggcggctcc actccaccgc ctgccagccc tcttccatgg tgcgcggcag
     1261 gtaatccatg cgcgccgtga gcacgctcac ggtgccgggc accagctccg cagggcgggc
     1321 ccgtttgagg ccgtggctgg ccatataggc catgtcaccg tggaaacctt gcgacagcca
     1381 ggccaataaa ccgggctctg ccgacgacaa atccacatcg gccacgccaa tttgggagaa
     1441 tccaagttcc cgggcccagg cctgtatctg agaaaccagc tggttgccat cgaattgagt
     1501 gctgttgatc gtcaccaccc cattgtagaa actgcagtgc gtaccctgct ttggtccaca
     1561 gaagcggaca cgcaggtctt cgccatgcgg tgggcgcaca gtgctgccat tggcaacgcc
     1621 tttgtcgccc tgcatggtga cctgggcgcc ggcaaaacca cgctggtgcg ccacctgctg
     1681 aaggcgctgg gcgtgcaggg ccgtgtcaaa agcccgacct atgcggtggt cgaaccctac
     1741 gaactgcccg caatgaacgt ctggcacttc gacttctacc gctttaccga cccgcgcgag
     1801 tgggaagacg ccggctttcg cgacatcttt gccagccacg gcctcaaact ggccgaatgg
     1861 cccgaaaaag ccgccgccat gctaccagtg gccgacgtgg acgtgcagat tcgcaccctg
     1921 gacgatgcaa cccggcaagt gacattgacg gcccacaccg ccaccggt
//
LOCUS       DAOSJM010000417         1964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_50063_length_1964_cov_4.775799, whole genome shotgun sequence.
ACCESSION   DAOSJM010000417 DAOSJM010000000
VERSION     DAOSJM010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50063_length_1964_cov_4.775799"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PKX82_12105"
     CDS             complement(<1..781)
                     /locus_tag="PKX82_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOF53042.1"
                     /translation="MNFSNTKISVKLGGAFLLMVLLTAIIGGFSLIQLARINGNTEEM
                     ASNWLPSIKYAGEIQGLLYDLRQAETQHAMAVAPEDKKIEADRIAANKGRIHEVGTKL
                     SALLDSSTEKQVWEKYGKDLAAYYAISTKLLVLSDVGPAEAMATTELLNGESRTAFKA
                     LSKDIEETIASHSKGADAAHAGAKQTYAGSMWSVIVLLVVTIALAAAMAYWITRQITQ
                     PIQYAVSAAKEFASGNLTHALHPRGKDEPAQMLHALESMRLA"
     gene            1001..>1964
                     /locus_tag="PKX82_12110"
     CDS             1001..>1964
                     /locus_tag="PKX82_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOF53043.1"
                     /translation="MKSSRFRVHPSWIIVLAGVSAALHVGKLPPALPVLQVALGISLV
                     QAGFLMSLVQMAGMALGLLVGLGADSLGLRRCMLVGLAVLSTASFLGGCADHLAALLF
                     LRGVEGLGFLLVVMPAPTLIRRTVALEALGPRLGWWGAYMPLGSAIALLIGPWVLADW
                     HWSVWWWSLGLVSALAWVAVWWAVPELAAVRPAAQGPVTRSEWLPRLRLTVRSPGPWL
                     VALCFAAYSAQWMAVIGFLPLVYAQMGLAAGITGVLTAAVALVNVSGNVLSGRLVQRG
                     WSAATVLQLGFAAMAVGAVGAFGQWQGEGLVLPLRFASVVFFSAV"
BASE COUNT          290 a          523 c          655 g          496 t
ORIGIN      
        1 gagccaaccg catggactcc agtgcgtgca gcatctgtgc gggctcgtcc ttaccgcgcg
       61 gatgcagggc atgggtcaga tttccgctcg caaactcttt tgcggcactg acggcgtatt
      121 gaatgggctg ggtgatctgg cgggtgatcc agtaggccat ggctgcggcc agggcgatgg
      181 tgacgaccag cagcacgatc acgctccaca tggagccagc ataggtttgc ttggccccgg
      241 catgtgcagc gtcagcgccc ttggagtggg acgcaatggt ttcttcaatg tctttggaca
      301 gggctttgaa agcggttcgt gactcgccat tgagcagctc ggtggtggcc atggcctccg
      361 cgggaccaac gtcggacagc accagcagtt tggtgctgat ggcgtagtag gcagccaggt
      421 ccttgccgta tttttcccag acctgttttt ccgtcgaaga gtccagcaag gccgacagtt
      481 tggtccccac ttcatggatc ctgcccttgt tggcagcaat gcggtccgcc tctatcttct
      541 tgtcctcagg cgcgacggcc atggcgtgct gcgtttctgc ctgacgcaag tcgtacagca
      601 gcccttgaat ttcacctgcg tacttgatac tgggcagcca gttgctggcc atttcctcgg
      661 tgttgccgtt gatgcgcgcc agctggatca gcgagaagcc gccaatgatc gccgtcagca
      721 gcaccatgag caaaaacgct ccgcccaact tgacgctgat tttggtattc gaaaagttca
      781 tgtactacgt tcccaacaaa tatgctttat tactcaggag taccctgcgc aaaactttcg
      841 aattggcgag gcattcaatc cgaccagtgt ggcataccac gcaccagatg gcgatccact
      901 tggtgacact ttttgcaggc tggggtttac cctgtggtga tttcttgcag aatgaatcca
      961 tctcccgctc tcccagcttt cttcgaatgt gccagcgccc atgaagtctt cccgttttcg
     1021 cgtccatccg tcgtggatca ttgtgctggc aggtgtcagt gccgcactgc atgtgggcaa
     1081 gctgccgcct gcacttccgg ttttgcaggt tgcgctcggc atatcactgg tacaggcggg
     1141 attcctgatg tctctggtgc aaatggcggg catggcattg ggcctgttgg tgggtcttgg
     1201 ggcggatagt ctggggctgc ggcgctgcat gctggtgggg ctggcggtgc tgtcgacggc
     1261 cagttttctg gggggctgtg cagaccatct tgccgctttg ctgtttctgc ggggtgtgga
     1321 aggcttgggc tttctgctgg tggtgatgcc ggcgcccacc ctgattcggc gcaccgttgc
     1381 gctagaagca ttggggccac gcctgggttg gtggggtgcc tacatgccgc tggggagtgc
     1441 catcgcactg ttgatcggac cttgggtgct ggcggactgg cattggtcgg tctggtggtg
     1501 gtcgcttggg ctggtatctg cgctggcctg ggtggctgtg tggtgggcgg tgccagagct
     1561 tgccgcggtg aggcctgccg cacaaggtcc tgtgaccaga tcggaatggt tgccgcggct
     1621 gcggctgacc gtgcgcagcc cggggccctg gcttgttgca ttgtgctttg ctgcgtattc
     1681 cgcccaatgg atggctgtca tcggttttct tccgttggtt tatgcgcaga tggggttggc
     1741 cgccggaatc accggtgtgc tgacggccgc tgtggcactc gtgaacgtga gtggcaatgt
     1801 actgtcgggc aggttggtgc agcggggctg gagcgccgcg acggtgctgc aactgggctt
     1861 tgcggccatg gctgtgggcg ctgttggcgc atttggtcaa tggcagggcg agggattggt
     1921 tttgccgctg cggttcgcgt ctgtggtgtt tttttccgca gtgg
//
LOCUS       DAOSJM010000418         1947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_50667_length_1947_cov_3.417548, whole genome shotgun sequence.
ACCESSION   DAOSJM010000418 DAOSJM010000000
VERSION     DAOSJM010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50667_length_1947_cov_3.417548"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PKX82_12115"
     CDS             <1..1021
                     /locus_tag="PKX82_12115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOF53044.1"
                     /translation="VPVELGGFGASIVELGYMCEALGQRCSSAAMVFAMHQIQVACMV
                     RHGTTPFFQNYMKDIVRDQRVIASVTSEAGVGGSVRTSICPIERDGDQCSIRKEGTVV
                     SYCADADDLLITVRRNAEAASNDQALILVNKSQCNLKVLSTWDSMGMRGTCSLGYVVD
                     ARFGEEQIVPDAFGDINSRTMVPWAHILWGSAWLGIATDAVARARAFVKDTGRKMGTT
                     PPTAARLSVVSTQLQTMRIQVRERAKYYNYLVSQPDGGLEVLASVDFAVQSNHLKLAA
                     SELVAEICTQALRITGTTGYRNDSPYSVTRNLRDAHSAALMIGNERIHAGNGQLHMMY
                     RDEAL"
     gene            complement(1038..>1947)
                     /locus_tag="PKX82_12120"
     CDS             complement(1038..>1947)
                     /locus_tag="PKX82_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOF53045.1"
                     /translation="VLVYQAGGCDGLIAVGGGSAIDCAKGVAIAATHEGPLTHYATIE
                     GGSPRITDQVAPIIAVPTTSGTGSEVARGAIIIVDDHRKLGFHSWNLVPKAAICDPEL
                     TLGLPPKLTAATGMDAIAHCMETFMAPAFNPPADGIGLDGLARGWAHIERATRDGSDR
                     EARLNMMSASMQGAMAFQKGLGCVHSLSHSLGGVDPRLHHGTLNAMFLPAVIAFNAQA
                     ESIRKENRLQRMAHAMGLASGSDIGEAIKDMNARLGLPKGLAEMGVTAAQFDAVIKGA
                     MADHCHKTNPRIATEDDYREMLAQSL"
BASE COUNT          349 a          596 c          612 g          390 t
ORIGIN      
        1 tgtgcccgtg gaactgggcg gatttggtgc cagcatcgtc gaattgggct atatgtgtga
       61 ggcgttgggg caacgctgct cctctgcagc catggtattt gccatgcacc agatccaggt
      121 ggcctgcatg gtgcgccatg ggaccacacc gtttttccag aactacatga aggacatcgt
      181 ccgcgaccag cgtgtgattg cctcggtgac ttccgaagcc ggcgtcggcg gttcggtgcg
      241 caccagtatc tgccccattg aacgcgatgg tgaccagtgt tccatccgca aagagggcac
      301 ggtcgtgtcc tactgtgcgg atgcggacga tctgctgatc accgtccggc gcaacgccga
      361 ggcggcctcc aacgaccagg cgctgatcct ggtgaacaag tcgcaatgca acctcaaggt
      421 gctcagcacc tgggactcca tgggcatgcg cggaacctgt agcctgggtt atgtggtcga
      481 tgcccgtttt ggcgaagagc agattgttcc agacgctttt ggcgatatca attcccgcac
      541 catggtgccg tgggcgcaca ttctgtgggg ctcggcctgg ctgggcatcg ccacggatgc
      601 cgtggcccgt gcccgcgcct ttgtgaaaga tactggccgc aagatgggca ccacaccacc
      661 taccgcagca cgtctgagtg tggtctccac ccagctgcaa accatgcgca tccaggtgcg
      721 ggagcgtgcc aagtactaca actatctggt cagccagcct gacggtggac tggaagtgct
      781 ggcgtcggtg gactttgcgg tgcaatccaa ccacctgaaa ctggcggctt ccgagctggt
      841 agccgaaatc tgtacccagg cactgcgcat taccggcacc accggctacc gcaacgattc
      901 gccctacagc gtgacgcgca acctgcgcga tgcccactcc gccgccttga tgatcggcaa
      961 cgagcgcatc cacgcgggca acggccagct gcatatgatg tacagggacg aggccctgta
     1021 agcatcgcac tgcgcagtta tagcgactgc gccagcatct cccggtaatc gtcctcggtg
     1081 gcgatacggg ggttggtctt gtggcagtgg tcggccatgg cgcctttgat gaccgcatca
     1141 aattgcgccg cggtgacacc catctcggct agccctttgg gtagccccaa acgggcattc
     1201 atgtccttga tggcttcgcc gatatcgctg ccgctggcca gacccatggc atgggccatg
     1261 cgctgcaggc ggttttcttt tcggatcgat tcggcttgtg cattgaacgc aatcacagcc
     1321 ggcaggaaca tggcattgag cgtgccgtgg tgcaggcgcg ggtccacgcc ccccagactg
     1381 tggctcagcg aatgcacgca gcccagacct ttctggaacg ccatggcacc ctgcatgctg
     1441 gcgctcatca tgttcaggcg cgcttcgcgg tcgctgccgt cccgggtggc gcgttcgata
     1501 tgtgcccagc cacgtgccag accatccagg ccaataccgt ccgctggcgg attgaaggcc
     1561 ggcgccatga aggtctccat gcaatgtgca atggcgtcca tgccggtggc ggcggtgagt
     1621 ttgggtggca gcccaagggt gagttcaggg tcgcagatgg ccgccttggg caccagattc
     1681 cagctatgga atccgagctt gcggtggtcg tccacaataa tgatggcacc gcgtgccact
     1741 tcgctgccgg taccgctggt ggtgggcacc gcaatgatgg gtgcgacttg gtccgtgatg
     1801 cgcggcgatc cgccttcgat ggtggcgtag tgggtcagcg gcccttcgtg ggtggcggca
     1861 atggccacac ctttggcgca gtcgatagca gaaccgcccc cgacggcaat cagtccatca
     1921 cagccaccgg cctggtagac cagtacc
//
LOCUS       DAOSJM010000419         1937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_51054_length_1937_cov_3.520723, whole genome shotgun sequence.
ACCESSION   DAOSJM010000419 DAOSJM010000000
VERSION     DAOSJM010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51054_length_1937_cov_3.520723"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1348)
                     /locus_tag="PKX82_12125"
     CDS             complement(join(120..1001,1004..1348))
                     /locus_tag="PKX82_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612240.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOF53046.1"
                     /translation="MESSVKRTQRDYTLAFNLAVVEQVEKGELTYKQAQQHYGIQGRS
                     TVLVWLRKHGRLGWSPAASAAAMPIDKKNTGALTPEQQIKALQVQLAQAQEKAKLFEA
                     VIDVLRKDYGVRIKKAFGQVLSQNLVTGLSVKRACQHMGHSRQAYYQGRRRQTDRIAN
                     AQTVVELVREQRMRQPRIGTRKLHHLLQEPLQRQAIALGRDAMFDVLRNARLLVAPRR
                     AYHKTTDSHHRFRRHPNLLKAGPQQVRASASEQVWVADITYLPTHGKFVYLSLVTDAY
                     SRKIVGWHVHESLQTEEVAQAMKMALSGRQSNRQLVHHSDRGIQYCATYYQALHKRHG
                     VICSMTDGYDCYQNALAERVNGILKSEFLLQRPADLGQARRMVEQSVDIYNCERPHLS
                     LKMQTPDAVHRASLAG"
     gene            complement(1465..1719)
                     /locus_tag="PKX82_12130"
     CDS             complement(1465..1719)
                     /locus_tag="PKX82_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOF53047.1"
                     /translation="MKEGIHPNYREVLFVDLSNGFKFVTRSCVNTKETETHEGKEYPL
                     FKLDTSSESHPFYTGTQKSVDNMGGRVERFRNRFGKTAAK"
BASE COUNT          350 a          582 c          573 g          432 t
ORIGIN      
        1 aacagcagcg acggagagga aacgagcggt cttgttcatg atgaaaatcc ttgaaggtta
       61 aaaaactgac ccgtcctgaa tagcgttgac acccaggccg gttaaaaact ccagccggac
      121 tatccggcca atgacgcccg atgcactgca tcgggcgttt gcattttcag cgacagatgc
      181 ggtcgctcgc agttgtagat gtccactgac tgctcgacca tccgtctggc ctgccccaga
      241 tcggcggggc gctgcagcag gaactcactc ttcaagatgc cattgacccg ctcggccagc
      301 gcgttctggt agcagtcgta gccgtccgtc atcgagcaga tcacgccgtg gcgcttgtgc
      361 agggcctggt agtacgtcgc gcagtactgg atacccctgt cggagtggtg caccaactgc
      421 cgattgctct gtcgccccga gagcgccatc ttcatggcct gcgccacctc ttcggtctgc
      481 aggctctcat gcacgtgcca gccgacgatc tttctggaat aagcatccgt gaccaggctc
      541 aggtaaacaa acttgccgtg tgtgggcagg taggtgatgt cagccaccca gacctgttcg
      601 ctggcgctgg cacgaacctg ctgtggcccg gctttgagca ggttcggatg gcgccggaag
      661 cggtggtgac tgtctgttgt cttgtggtac gcccgccggg gcgccaccag cagcctggca
      721 ttgcgcagga cgtcgaacat cgcatcgcgg cccagggcta ttgcctggcg ctgcagcggc
      781 tcctgcagca agtgatgcaa cttgcgtgtg cctatgcggg gctgacgcat gcgctgctcg
      841 cgcaccaact ccaccaccgt ctgcgcattc gcgatgcgat cggtctggcg gcgcctaccc
      901 tggtagtacg cctgacggct gtggcccatg tgctggcaag ccctcttcac gctcaaccct
      961 gtgacaaggt tttgcgagag gacttgccca aaggcttttt tacgatgcgc accccatagt
     1021 ccttgcgcag cacgtcgatg acggcttcaa atagcttggc cttctcctgc gcctgtgcga
     1081 gctgcacctg cagtgccttg atctgctgct cgggcgtgag agccccagtg ttcttcttgt
     1141 ctattggcat ggctgcagct gatgccgcag gactccagcc caggcgaccg tgcttgcgca
     1201 gccagaccag aaccgtcgag cggccctgga tgccgtagtg ctgctgcgcc tgtttgtacg
     1261 tgagctcgcc tttttccacc tgctccacca ccgccaagtt aaaggccagc gtgtagtcgc
     1321 gctgcgtgcg tttgacactt gattccatga cacatgccct ttttgaaagg tccatgtgtc
     1381 aacgccgggt aggacgggtc aaacccatga aaaaaggcag cccggttgtc cgggctgcct
     1441 ttttggcggg atcgcagggg cgaattactt cgctgcggtc ttgccgaaac ggttgcggaa
     1501 gcgttccacg cggccaccca tgttgtccac cgacttttgc gtgccggtgt agaaggggtg
     1561 cgactcagaa gaggtatcca gcttgaacag cgggtattcc ttgccttcgt gggtttcggt
     1621 ttccttggtg ttcacgcacg agcgggtcac gaacttgaaa ccgttggaca ggtccacgaa
     1681 cagaacttcg cggtagttgg ggtgaatgcc ttctttcatg atcaatcctt gtcaagtgcg
     1741 ggagccgtgc gggacccgaa gcaacctttg cctcgtgaaa catccagcgt actttccgca
     1801 gaaaaagccc acgattatgg cataggcgcg gttgacgtca ttttgggtgg attgctgcgc
     1861 cacttttccc gtggcgctta acttcgagcc gcgctcatgc cactgccacc gtggaactgg
     1921 ctctgccagg ccaccgg
//
LOCUS       DAOSJM010000420         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_51520_length_1924_cov_3.299090, whole genome shotgun sequence.
ACCESSION   DAOSJM010000420 DAOSJM010000000
VERSION     DAOSJM010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51520_length_1924_cov_3.299090"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="PKX82_12135"
     CDS             <1..851
                     /locus_tag="PKX82_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOF53048.1"
                     /translation="LTKYDLSSLRALFLAGEPLDEPTAQWISGSLKKPIIDNYWQTET
                     GWPILGIANGVEQKASKFGSPGVPMYGYNVMLMDDAGEELVGGDKKGVVMVEGPLPPG
                     CMQTVWRDDDRFVKTYWQKLNGKYVYSTFDWGVRDADGYYFILGRTDDVINVAGHRLG
                     TREIEEAISSHPNISEVAVVGVADNLKGQVAVAFAVVKDASVLVDDAAHAKLEAEVMK
                     VVDNTIGAVGRPARVRFVAVLPKTRSGKLLRRAIQAVCEGRDPGDLTTMEDPAALQQI
                     KDLTAA"
     gene            1044..>1924
                     /gene="sucA"
                     /locus_tag="PKX82_12140"
     CDS             1044..>1924
                     /gene="sucA"
                     /locus_tag="PKX82_12140"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464607.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOF53049.1"
                     /translation="MSETTSPGANQPASVYQAYQNNTYLFGGNAPYVEEMYENYLANP
                     GSVPDSWRDYFDALQHVPAVDGSSARDVAHLPVINAFAERAKQGVKHVVMATGADSEM
                     GRKRTAVQQLIAAYRNVGARWADLDPLKRTERDKIPELELSFYGFNDADQETVFNISN
                     TFFGKESMSLRELMNALRETYCGTIGAEYMYTTDQNQKRWWQQKLESIRSKPQFNAEK
                     KRHILDRLTAAEGLERFLHTKYVGQKRFSLEGGESFIAAMDELIQQAGAKGVQEIVIG
                     MAHRGRLNVLVNTLGKMP"
BASE COUNT          433 a          556 c          582 g          353 t
ORIGIN      
        1 tgctgaccaa atacgacttg tccagcctgc gggccctgtt cctggcgggc gagccgctgg
       61 acgagccgac cgcgcaatgg atcagcggca gcctgaaaaa acccatcatc gacaactact
      121 ggcaaaccga aacgggctgg ccgattctgg gcattgccaa cggcgttgag caaaaagcca
      181 gcaagtttgg cagccctggc gtgcccatgt atggttacaa cgtcatgctg atggacgatg
      241 cgggggaaga actggtgggt ggcgacaaga agggcgtggt gatggtggaa ggtcccttgc
      301 cccccggttg catgcaaacc gtgtggcgcg atgatgaccg ttttgtcaaa acctactggc
      361 aaaagctcaa cggtaaatac gtttacagca cctttgactg gggtgtgcgt gatgccgatg
      421 ggtactactt tatcctgggt cgtaccgacg atgtgatcaa cgtggcgggt caccgcctgg
      481 gcacccgcga gatcgaggaa gccatttcca gccatcccaa tatctccgaa gttgctgtgg
      541 tgggggtagc cgacaacctc aagggccaag tggccgtggc cttcgccgtg gtgaaggacg
      601 ccagtgtcct ggtggacgat gcagcccatg ccaaactgga agcggaagtc atgaaggtgg
      661 tggacaacac catcggcgcc gtgggccggc cggcacgcgt gcgctttgtg gcagtcctgc
      721 ccaagacccg cagtggcaaa ttgctgcgcc gtgccattca ggcagtctgc gaggggcgtg
      781 accccggtga tctgactacg atggaagacc cggccgcgct gcaacagatc aaggacctta
      841 ctgcagcctg atagtgtcaa ttggcactct gctgcagtac ggtacttcgt gtttgcggaa
      901 ttcggtggca caatcgaacc cgcaatttag gcccttccca tgccacagtc gcatccgcca
      961 aacggttcag ccgtgtcgcg gaaggttaat caacagcttt aaccagctaa tgttccccca
     1021 aggggaacgg aggtcagcgg aatatgagtg agacaacgtc ccccggggcg aatcagcctg
     1081 cgtctgtgta tcaggcgtac cagaacaata cctacctgtt tgggggcaat gccccgtacg
     1141 tcgaagagat gtacgaaaac tacctggcca atccaggcag cgttccagac tcctggcgcg
     1201 attacttcga cgccctgcag catgttcccg cagtggacgg cagtagcgcc agagacgtag
     1261 cccatcttcc cgtcatcaat gccttcgccg agcgcgccaa gcagggcgtg aagcatgtgg
     1321 tcatggccac cggagccgat tccgagatgg gccgcaagcg cacggcagtg cagcaactga
     1381 ttgctgccta ccgcaatgtg ggcgcacgct gggccgatct ggacccgctc aagcgcaccg
     1441 agcgcgacaa gattcccgaa ctcgaactgt ctttttacgg cttcaacgat gccgaccagg
     1501 aaaccgtgtt caacatcagc aacacgttct tcggcaagga gtcgatgagc ctgcgtgagc
     1561 tgatgaatgc cctgcgtgaa acctattgcg gcaccattgg cgccgagtac atgtacacga
     1621 cggaccagaa ccagaagcgc tggtggcagc agaagctcga aagcattcgc agcaagcccc
     1681 agttcaacgc cgagaaaaag cgtcacatcc tggaccggct gactgctgcc gaaggcctgg
     1741 agcgattcct ccacacgaaa tatgtgggcc agaagcgatt ctcgctcgaa ggcggtgaga
     1801 gctttatcgc cgccatggac gagctgatcc agcaagccgg cgccaagggc gtgcaggaaa
     1861 tcgtgatcgg catggcccac cgtggccgtc tgaacgtgct ggtcaacacc ctgggcaaga
     1921 tgcc
//
LOCUS       DAOSJM010000421         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_52325_length_1903_cov_4.633658, whole genome shotgun sequence.
ACCESSION   DAOSJM010000421 DAOSJM010000000
VERSION     DAOSJM010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52325_length_1903_cov_4.633658"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PKX82_12145"
     CDS             <1..781
                     /locus_tag="PKX82_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOF53050.1"
                     /translation="LQIFGDGPVKVAVVEVQPDLSLRATATVTEAVSGDSRLSQMVNV
                     NNQGRCAITLDPKTKFPGQQPYQGVVPLFDDHGRPIEKLSAVLEHYMLQSEQLDTTLV
                     LAANDTVAAGLLIQRLPMAGAGNLAGSMVSKENEDQIGLNEHYNRIAMLASSLKQEEL
                     LTLDVDTILRRLFWEEQITRFEPLMGETAPRFVCSCSRERVAKMIQSLGREEAESILE
                     ERGDIEVACEFCGVQYRFDPVDAAQLFTDSARLGQSGTDLH"
     gene            complement(778..1410)
                     /locus_tag="PKX82_12150"
     CDS             complement(778..1410)
                     /locus_tag="PKX82_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOF53051.1"
                     /translation="MRIAILGAECTGKTSLAKALREHLHQPQSPWILFPELLREWCVQ
                     HQRTPRAEEQEGLLQTQLQHMSEGMQANTSAIADTTPLMTAIYSDLLFQDTSLYACAL
                     EHQRIYDLTLVTAPDIDWQADGFQRDGIATRAAVDRKLRSVLQGSAIPYAVICGTGEA
                     RTQSALDAIAHWQRTPRPRNTHDTEWKWSCDTCSDPQCEHRLFSRLLGSS"
     gene            complement(1432..1707)
                     /locus_tag="PKX82_12155"
     CDS             complement(1432..1707)
                     /locus_tag="PKX82_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOF53052.1"
                     /translation="MGNRVVPAALIALLVILHGQLWFGRGSVPNVSRLQGQLAEQKRK
                     NVQAAQANDRLSAEIHDLRDGLEIVEEKARSELGMVRANEIYVQIAK"
     gene            complement(1707..>1903)
                     /gene="eno"
                     /locus_tag="PKX82_12160"
     CDS             complement(1707..>1903)
                     /gene="eno"
                     /locus_tag="PKX82_12160"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOF53053.1"
                     /translation="SHRSGETEDSTIADIAVGTNAGQIKTGSLSRSDRMAKYNQLLRI
                     EEDLGDVAFYPGRAAFYNLK"
BASE COUNT          357 a          533 c          610 g          403 t
ORIGIN      
        1 attgcagatt ttcggcgacg gtccggtcaa ggttgcagtg gtggaggtgc aacccgatct
       61 gtccctgcgt gcgaccgcta ccgttaccga ggccgtttcc ggagacagcc ggttgagcca
      121 gatggtcaac gtgaacaacc agggacgttg cgccatcacg ctggatccca agaccaagtt
      181 ccccggtcag caaccgtacc agggtgtggt gcccctgttt gacgaccacg ggcgtcccat
      241 cgaaaaactg agcgctgtgc tggagcacta catgctgcaa agcgagcagc tcgacacgac
      301 actggtactg gcggccaatg acacggtggc cgccggcctg ctgatccagc gcctgccaat
      361 ggccggtgcc ggcaatctgg cgggcagcat ggtgtcgaag gaaaacgaag accagattgg
      421 tctgaacgag cactacaacc ggattgccat gctggccagt agcctgaagc aggaagagct
      481 gctgacactg gatgtggaca ccatcctgcg gcgtttgttc tgggaagaac agatcacacg
      541 gtttgagccg ctgatgggtg aaactgcgcc ccggtttgtc tgttcctgca gccgcgagcg
      601 tgtggccaag atgatccagt cgctggggcg ggaagaagca gagtccatcc tggaagagcg
      661 cggcgacatc gaggtggcct gcgagttctg tggagtgcag taccggtttg acccggtgga
      721 tgctgctcag ttgtttaccg attcggcccg cctgggtcaa agcggcaccg acctgcacta
      781 gctgctgccg agcaatcgac tgaacaggcg gtgctcacat tgcggatctg agcaggtgtc
      841 gcaggaccat ttccactccg tgtcgtgcgt gttgcgtgga cgtggcgtgc gttgccagtg
      901 ggcgattgcg tccagtgcac tttgggtacg tgcttcgccg gttccgcata tgactgcata
      961 gggaatcgca ctgccctgca gaacgctccg cagttttcgg tcaaccgcgg cacgggtagc
     1021 aatgccgtca cgctgaaaac cgtcggcctg ccagtcaatg tcaggtgctg ttaccagcgt
     1081 aaggtcatag atgcgctgat gttcgagtgc gcaggcgtac agcgatgtgt cctggaagag
     1141 caggtcgctg taaatggcgg tcatcagcgg cgtggtatcg gcgattgcgg aagtgttggc
     1201 ttgcatcccc tcagacatgt gctgaagctg ggtctgtaac agcccttcct gctcttccgc
     1261 acggggtgtc cgctggtgtt gcacacacca ctcccgaagc agctcaggga acaggatcca
     1321 gggcgattgg ggttggtgga ggtgctcccg taacgccttt gccagcgagg ttttgccggt
     1381 gcactccgcg cccaatatgg ctatgcgcat gccggactcc tgcggatcag gtcacttggc
     1441 aatctgcacg tagatttcgt tggcgcgcac catgccgagt tcggagcggg ccttttcttc
     1501 cacaatctcc aggccgtcgc gcaggtcatg gatttcggcg gaaagacggt cattggcctg
     1561 tgcggcctgc acatttttgc gtttttgttc tgcgagttgt ccctgcaacc tggagacatt
     1621 ggggacgctc cctctgccga accacagttg cccgtgcagg atcaccagca aggcaatcaa
     1681 tgcagcggga acgactcggt tgcccattac ttcaggttgt agaacgcagc gcggccaggg
     1741 tagaaggcca catcaccgag gtcctcttcg atgcgcagca actggttgta cttggccatg
     1801 cggtcggaac ggctcaggga accagtcttg atctggcctg cgttggtccc cacggcgatg
     1861 tcggcgatgg tggagtcttc ggtttcgccc gagcggtggc tga
//
LOCUS       DAOSJM010000422         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_52985_length_1886_cov_4.756417, whole genome shotgun sequence.
ACCESSION   DAOSJM010000422 DAOSJM010000000
VERSION     DAOSJM010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52985_length_1886_cov_4.756417"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..486)
                     /locus_tag="PKX82_12165"
     CDS             complement(190..486)
                     /locus_tag="PKX82_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HOF53054.1"
                     /translation="MHKSVLTAMVAAALLAGCANQRFNVAGEMGVNPTPKAEDSQSFF
                     VNGIGQKQTIDAAKVCGGAAKVEGVAVEQSGMDVLLGMVTFGIYTPRTARVYCK"
     gene            complement(553..>1886)
                     /locus_tag="PKX82_12170"
     CDS             complement(553..>1886)
                     /locus_tag="PKX82_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOF53055.1"
                     /translation="LLVALPETEDQVAAVLRVCHTLNVPVVARGAGTGLSGGAMPHPL
                     GVTLSLAKFNRILKIDKRSRTAVVQCGVRNLAISEAAAPYGLYYAPDPSSQIACTIGG
                     NVAENSGGVHCLKYGLTLHNIVKVRGFTMEGEPVAFGSDALDQPGYDLLSIVVGSEGM
                     LAVTTEVTVKLVPKPQLARCIMASFDDMRKAGDAVASVIAAGIIPAGLEMMDKPMTAA
                     VEDFVHAGYDLTAEAILLCESDGTPEEVEEEIGRMSAVLTRCGATAIAVSKDEAERLR
                     FWSGRKNAFPASGRISPDYMCMDSTIPRKRLADILLAIAEMEKKYQLRCANVFHAGDG
                     NLHPLILFDANDPDQLRRCELFGADILETSVAMGGTVTGEHGVGVEKLNSMCVQFSAE
                     ENAQMFGIKHAFDTKELLNPGKVIPTLHRCAEYGKMLVRAGQIKHPDLPRF"
BASE COUNT          350 a          553 c          595 g          388 t
ORIGIN      
        1 cccgtatgct ggtggcgggc caatgtccgg ccggtttcca gggcgtaatc gatggagagg
       61 tgtgcgtgcc aggacatggg agttgatggg gtcagacggt ttcagtgttg caattggcgt
      121 gcctggatgt gctgaaacca aacaaaaagc ccctcatcga ggggcttgat tgtgcaggca
      181 atccgatgat tacttgcaat acacgcgcgc agtacgggga gtgtaaatac cgaaagtgac
      241 catgcccagg agtacgtcca tgccgctttg ttcgactgca acgccttcca ccttcgcggc
      301 accaccgcag actttggctg catcgatggt ttgcttctgg ccaatgccgt tgacgaagaa
      361 ggactggcta tcttctgcct tgggggttgg attgacgccc atttcaccag caacgttaaa
      421 acgctggttg gcgcaacctg ccagcagtgc ggcagcgacc atggcggtca gaacggattt
      481 atgcatcgaa atatctccct gaaagttgtc atgcccgcga ttcgggcaag accattatag
      541 ggaggccaat tctcaaaatc gtggcagatc cgggtgcttg atctgaccgg cgcgcaccag
      601 catcttgccg tactcggcgc agcggtgcag ggtggggatt accttgcctg ggttgagcaa
      661 ttctttggtg tcaaacgcgt gcttgatgcc aaacatctgc gcgttttctt cggcggaaaa
      721 ctgcacgcac atgctgttga gtttttccac gcccacaccg tgttcgccgg tgacggtgcc
      781 gcccatggcc acgctggtct ccagaatatc ggcgccaaac agctcgcagc ggcgcagctg
      841 atccgggtcg ttggcatcga acaggatcag cgggtgcagg ttgccgtcgc cggcgtggaa
      901 cacgttggcg cagcgcaact ggtatttctt ttccatctcc gcaatggcca gcaggatgtc
      961 ggccaggcgt ttgcgcggga tagtgctgtc catgcacatg tagtcggggc tgatgcggcc
     1021 ggatgccggg aacgcgttct tgcggccgct ccagaagcgc aagcgttcgg cctcgtcctt
     1081 gctaacggca attgcagtgg caccgcagcg ggtgagcacc gcgctcatgc ggccgatttc
     1141 ttcttccact tcctcgggcg tgccatcgct ctcgcacagc aggatggctt ccgccgtcag
     1201 gtcgtagcct gcgtgcacaa agtcttccac ggcagcggtc atgggtttgt ccatcatctc
     1261 cagacccgcg ggaatgatgc cagcggcgat gacggaggcg actgcgtcac cggctttgcg
     1321 catgtcgtcg aagctggcca tgatgcagcg cgccaattgg ggcttgggca ccagcttgac
     1381 ggtgacctcg gtggtgacag ccagcatgcc ttcgctgccg accacgatgc tgagcaggtc
     1441 atagccgggc tggtcgagtg catcgctgcc gaacgcaacg ggctcacctt ccatggtgaa
     1501 gccacgcacc ttgacgatgt tgtgcagggt caggccgtat ttgaggcaat gcacaccacc
     1561 cgagttttcg gccacgttgc cgccgatggt gcaggcgatc tggctgctgg ggtccggtgc
     1621 gtagtacaga ccatagggcg cagcagcctc gctgatagcc agattgcgca cgccgcactg
     1681 caccacggcg gtccggctac gcttgtcgat cttgagaatg cggttgaact tggccaggga
     1741 cagcgtgacg cccaaggggt ggggcatggc accgcccgac agcccggtac ctgcgccacg
     1801 ggccaccacc ggcacgttca gcgtgtggca aacccgaagc acggccgcca cctggtcctc
     1861 ggtttcgggc aaggccacca gcagcg
//
LOCUS       DAOSJM010000423         1879 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_53271_length_1879_cov_4.367325, whole genome shotgun sequence.
ACCESSION   DAOSJM010000423 DAOSJM010000000
VERSION     DAOSJM010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53271_length_1879_cov_4.367325"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PKX82_12175"
     CDS             <1..1032
                     /locus_tag="PKX82_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF53056.1"
                     /translation="VEKLFVVLKKLASEGCSILYISHKLHEIRELCTACTVLRGGKVT
                     GVCNPQEESNASLSRLMIGAEPPLLERNARTAGAAAMHVKGLNLEREDQFGVDLQDIH
                     LHVRSGEVVGIAGVSGNGQKELLYALSGEDCRAPRGSIHMGETDVSRLHPGERRKLGL
                     HFVPEERLGRGAVPTLSLAHNMLLTRTESIAYPKFAGGWIRVKPLEDHAARIIQAFNV
                     KAGGPQAVAKSLSGGNLQKFIVGREIDAKPTLFIVSQPTWGVDVGAAAQIRGEILALR
                     DAGCAVLVVSEELDELFEVCDRLHVIARGKLSPSVPMAEATVEKIGEWMSGLWETAAA
                     THGAEAQHA"
     gene            1025..>1879
                     /locus_tag="PKX82_12180"
     CDS             1025..>1879
                     /locus_tag="PKX82_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOF53057.1"
                     /translation="MLKVKLEARQQPSKFWSYGSPVLALLCTVVIGVILFAVLGKDPV
                     RGLQMFFWEPIKSGYALGELVVKATPLLLIALGLAVSFRSNVWNIGAEGQYIIGAVAA
                     SGIALMAEKTTGGWIVVPIILAGMLGGMVWAGLTAFLRDKFNANEILVSLMPVYVAVQ
                     VLGYMVYGPWKDPAGYNFPQTKNFDLVTRIPRLFEGSRLSIGVLFALVGVFGMWIFLF
                     RTRAGFAQQVGGLAPAASRYAGFSSRKALWTALLISGGMAGLAGALEVAGPIGQLTPY
                     VPAGYGFAA"
BASE COUNT          299 a          541 c          671 g          368 t
ORIGIN      
        1 gtcgagaaac tgtttgtggt gctcaagaag ttggccagcg agggctgcag cattctgtat
       61 atctcccaca agctgcacga aatccgcgag ctgtgcacgg cctgcacggt gctgcgcggc
      121 ggcaaggtga ccggtgtgtg caacccgcag gaggaatcca atgcctcgct gtcgcgcttg
      181 atgattggtg ccgagccgcc gctgctggag cgcaacgcgc gcaccgcggg cgctgcggcc
      241 atgcatgtca aagggctgaa cctggagcgc gaagaccagt ttggtgtgga cctgcaggac
      301 attcacctgc atgtgcgctc gggcgaggtg gtgggcattg ccggtgtctc cggcaacggc
      361 cagaaagagc tgctgtacgc gctgtcgggc gaagactgcc gggcgccgcg cggcagtatc
      421 cacatgggtg aaaccgatgt ttcgcgcctg catccgggcg agcggcgcaa gttgggcctg
      481 cactttgtgc cggaagaacg gctgggccgc ggtgcagtgc cgacgctgag tctggcgcac
      541 aacatgctgc tcacgcgcac cgagtcgatt gcgtacccga agtttgccgg cggctggatt
      601 cgcgtcaagc cgctggagga ccatgccgcg cgcatcatcc aggcgttcaa cgtgaaggcc
      661 ggtgggccgc aggccgtggc caagtcgcta tcgggcggca acctgcagaa gttcatcgtc
      721 ggccgcgaga ttgacgccaa gcccacgctg ttcatcgtct cgcagccgac ctggggtgtg
      781 gacgtgggcg cggcggcaca gattcgcggc gagattctgg ccttgcgtga tgcgggttgc
      841 gcggtgctgg tggtcagcga agagctggac gagttgttcg aagtgtgcga tcgcctgcat
      901 gtgattgcgc gcggaaaact gtcgccctcg gtgccgatgg ccgaggccac ggtggaaaag
      961 attggagagt ggatgagtgg actctgggaa accgctgcgg ccacgcacgg agcggaggcg
     1021 cagcatgctt aaggtaaagc ttgaagcgcg ccagcaaccc tcgaagttct ggagctacgg
     1081 ttcccccgtg ctggcactgc tgtgcacggt ggtcatcggg gtcatcctgt ttgcggtgct
     1141 gggcaaggac ccggtgcgcg gcctgcagat gtttttctgg gagcccatca agagcggcta
     1201 tgcgctgggc gagctggtcg tcaaggccac gccactgctg ctgattgcgc tggggttggc
     1261 agtgagcttt cgctctaatg tctggaacat cggcgccgag gggcagtaca tcatcggtgc
     1321 tgtcgcggcc agcggcattg cgctgatggc cgagaagacc actggcggct ggatcgtggt
     1381 gcccatcatc ctggcgggca tgctgggcgg catggtgtgg gcgggcctga ccgcattcct
     1441 gcgcgacaag ttcaacgcca atgaaattct ggtcagcctg atgccggtgt acgtagccgt
     1501 gcaggtgctg ggctacatgg tctatggccc ctggaaagac ccggccggct acaactttcc
     1561 gcagaccaag aatttcgatc tggtcacgcg catcccacgc ctgtttgagg gctcgcgcct
     1621 gtccatcggc gtgctgtttg ccctggtggg tgtatttggc atgtggatct ttctgttccg
     1681 cacccgagcc ggttttgccc agcaggtggg tggcctggcc cctgcggctt cgcgctacgc
     1741 cgggttctcc tcgcgcaagg cgctgtggac cgcgctgctg atttcgggcg gcatggccgg
     1801 tctggcgggc gcgctggagg tggccggacc gattggccag ctcacgccgt acgtgcccgc
     1861 gggctacggg tttgcggcc
//
LOCUS       DAOSJM010000424         1850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_54454_length_1850_cov_3.917549, whole genome shotgun sequence.
ACCESSION   DAOSJM010000424 DAOSJM010000000
VERSION     DAOSJM010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54454_length_1850_cov_3.917549"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKX82_12185"
     CDS             <1..628
                     /locus_tag="PKX82_12185"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HOF53058.1"
                     /translation="EWRSKRTGWITTNVAETQGFISTEGNTDYPNIQLALCTGIVDDH
                     TRKAHLGHGYTLHVTLMRPKSRGSVTLQSAKPTDAPLIDPAFFKDPDDMQTLIKATQM
                     GHDIMEAKALAPYRGKMIHPLERNNPTQIEAFLRKNSDTEYHPVGTCKMGPATDPMAV
                     VDASLKVHGMQGLRVVDASIMPRLTTGNTNAPSIMIGEKGAELIRAAT"
     gene            complement(621..1163)
                     /locus_tag="PKX82_12190"
     CDS             complement(621..1163)
                     /locus_tag="PKX82_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HOF53059.1"
                     /translation="MTSPTVADRYSRASIAMHWLMVAVFVGVYGAINLSELLDKGTAA
                     RQLARNWHSMLGLTVFALVWLRLALRLWGTTPAIVPAPPAWQEKLGKAVHGGLYLFMV
                     VTPLVGWALLTARGRVIPFYGLYLPDLPLPAMAQARLVKEVHEWLGNLGYALIGGHAV
                     AALWHHYGMRDSTLRRMWFR"
     gene            1284..>1850
                     /gene="gstA"
                     /locus_tag="PKX82_12195"
     CDS             1284..>1850
                     /gene="gstA"
                     /locus_tag="PKX82_12195"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HOF53060.1"
                     /translation="MKLYYSPGACSLSPHIAMYEAGLAFEAIMAPTKTHKLPDGSDYY
                     AVNPLGYVPFLTLDDGRSLHEGPAIVQYIADQAPEKNLAPANGTYERYKLQEWLTFIG
                     TELHRSFSPLFVPTTHPDIKATALERLQGRLKWVDGELAGKQYLMGDQFTVADGYLFV
                     VTNWAKPLKVDLSPFTNLLALRERVAARP"
BASE COUNT          401 a          662 c          490 g          297 t
ORIGIN      
        1 cgagtggcgc agcaagcgca cgggctggat caccaccaac gtggccgaaa cccaaggatt
       61 catttctact gagggcaaca ccgactaccc caacatccag ttggccctat gcaccggcat
      121 tgtcgacgac cacacccgca aggcccacct gggccacggc tacaccctgc atgtcaccct
      181 gatgcgcccc aagagccgtg gctcggtcac actgcaaagc gccaagccga cggacgcacc
      241 gctgatcgac cccgcgttct tcaaagaccc cgacgacatg cagaccctga tcaaggccac
      301 gcagatgggc cacgacatca tggaggccaa ggcgctggcg ccctaccgcg gcaaaatgat
      361 ccacccgctg gagcgcaaca acccgacaca aatcgaagcg ttcctgcgca aaaactccga
      421 caccgaatac catccggtcg gtacctgcaa gatgggcccc gcaacggacc cgatggcagt
      481 ggtggatgcc agcctcaagg tgcacggcat gcagggcctg cgcgtggtgg atgcatccat
      541 catgccgcgc ctgaccaccg gcaataccaa cgcccccagc atcatgatcg gggaaaaagg
      601 tgccgagctg attcgcgcag ctacctgaac cacatccggc gcagtgtgct gtcccgcatc
      661 ccgtagtggt gccacagcgc tgccacggcg tgccccccaa tcaacgcata gcccaaattg
      721 ccaagccatt cgtgcacttc cttgaccagc cgcgcctgcg ccatcgcggg caagggcaga
      781 tctggcagat acaggccata aaacggaatc accctgccgc gcgcagtcag caaagcccag
      841 cccaccaggg gcgttaccac catgaacagg tacaggccgc catgcacagc cttacccagt
      901 ttttcctgcc aggctggggg agccggcacg atggccggcg tcgtccccca caaccgcagc
      961 gccaggcgca gccataccag tgcgaatacc gtgaggccca gcatggaatg ccagttcctg
     1021 gccagctggc gcgccgccgt gcccttgtcc agcagttctg agagattgat ggcgccatag
     1081 acacccacaa acaccgccac catcaaccag tgcatggcaa tgctggcgcg gctgtatcgg
     1141 tcggcaacgg ttggtgaagt catgcatagc tccggcaatc agttggatat gtcactgtag
     1201 cgccacccca tgctgcaaac aaatccgttt gccggtagca tggttatctg catctctctt
     1261 ttcccaaccg tccattccat cccatgaagc tctactactc ccccggcgcc tgctccctgt
     1321 ccccacacat cgcgatgtac gaggccggtc tggccttcga agccatcatg gcacccacca
     1381 agacccacaa gctgcccgat ggcagcgact actatgctgt caacccgctg ggctatgtgc
     1441 ccttcctgac gctcgacgac ggtcgcagcc tgcacgaagg cccggccatc gtgcaataca
     1501 ttgccgacca ggcacccgag aaaaacctcg cccccgccaa tggcacctac gagcgctaca
     1561 agctgcagga atggctcacc ttcatcggca ccgagctgca tcgctcgttt tcacctctgt
     1621 tcgtccccac cacccacccg gatatcaagg ccaccgcact ggagcgcctg cagggtcgtc
     1681 tgaagtgggt cgatggagaa ctcgccggca agcagtacct gatgggcgac cagttcaccg
     1741 tggccgatgg ctacctcttc gtggtcacca actgggccaa gccgctgaag gtcgacctgt
     1801 cgcccttcac gaacctgctg gccctgcgcg aacgcgtggc ggcacgcccg
//
LOCUS       DAOSJM010000425         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_55230_length_1830_cov_5.375775, whole genome shotgun sequence.
ACCESSION   DAOSJM010000425 DAOSJM010000000
VERSION     DAOSJM010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55230_length_1830_cov_5.375775"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1830)
                     /gene="leuS"
                     /locus_tag="PKX82_12200"
     CDS             complement(<1..>1830)
                     /gene="leuS"
                     /locus_tag="PKX82_12200"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOF53061.1"
                     /translation="MLPYPSGKLHMGHVRNYTINDMLTRYLRMNGYNVLMPMGWDAFG
                     LPAENAALKNKVPPAKWTYENIAYMKGQMQAMGLAIDWSREVATCDPSYYKWNQWLFL
                     KMLEKGIAYRKTQVVNWDPVDQTVLANEQVIDGKGWRTGATVEKREIPGYYLKITDYA
                     QELLGHVQDGLPGWPERVKLMQENWIGKSEGVRFAFTHDIQDSQGQLIGDGRMYVFTT
                     RPDTIMGVTFCAIAPEHPLAVHAAQSNLKLAAFIEECKAGGTTEAELAVKEKLGMPTG
                     LQVTHPLTGRLVDVWVGNYVLMGYGDGAVMGVPAHDERDFAFAKKYNFPIHDVVHVDG
                     LTYDHAQWQDWYGDKQRGITVNSDVFSGLNYKEAVDAVAKALAAKGLGEKKTTWRLRD
                     WGVSRQRYWGTPIPIIHCEEHGAVPVPEKDLPVVLPQDCVPDGSGNPLHKHEGFHAGV
                     VCPVCGKPARRETDTMDTFVDSSWYFMRYCDPHNADAMVAGGADYWMPMDQYIGGIEH
                     AILHLLYARFWTKVMRDLGLVKVDEPFTKLLTQGMVLNHIYSRKGDKGGKEYFWPHDV
                     EHVLDDTGKVIGAKLKNAVGDLPVGTAIDYEGVGTMSKSKNNG"
BASE COUNT          303 a          597 c          536 g          394 t
ORIGIN      
        1 caccgttgtt cttggacttg gacatggtgc ccacgccctc gtagtcgatg gcagtgccca
       61 cgggcaaatc gcccacggcg ttcttcagct tggcaccaat gaccttgccg gtgtcgtcga
      121 gcacatgctc cacgtcgtgc ggccagaaat actctttgcc gcccttgtcc cccttgcggg
      181 aatagatgtg gttgagcacc atgccttgcg tcagcagctt ggtaaacggc tcatcgacct
      241 tgaccagccc cagatcccgc atgaccttgg tccagaagcg cgcgtacagc aggtgcagga
      301 tggcgtgttc gatgccaccg atgtactggt ccatcggcat ccagtagtcc gcacctcctg
      361 ccaccatggc atccgcattg tgcggatcgc aatagcgcat gaagtaccac gagctgtcca
      421 caaaggtgtc catggtgtcg gtttcgcgcc gtgcgggctt gccgcacacc gggcacacca
      481 cgccagcgtg gaaaccttcg tgcttgtgca aggggttgcc ggagccgtcg ggcacgcagt
      541 cctgtggcag caccacgggc aggtctttct cgggcaccgg cactgcgccg tgctcctcgc
      601 aatggatgat cgggattggc gtgccccagt agcgctggcg gctcacaccc cagtcgcgca
      661 ggcgccaggt ggtttttttc tcgcccagac ccttggcagc cagtgccttg gcgactgcgt
      721 ccaccgcctc cttgtagttc agcccgctga acacatcgga gttgacggta atgccacgtt
      781 gcttgtcgcc ataccagtcc tgccactgcg cgtggtcata ggtcaggccg tccacatgca
      841 ccacgtcgtg gatcgggaag ttgtatttct tggcgaacgc aaagtcacgc tcgtcgtggg
      901 caggcacccc catcaccgcg ccgtcgccat agcccatcag cacgtagttg cccacccata
      961 cgtccaccag acgcccggtc agcgggtgcg tgacctgcag gcccgtgggc atgcccagct
     1021 tttccttgac cgcgagttcg gcctcggtgg ttcctccggc cttgcattct tcgatgaagg
     1081 ctgccagctt caggttggac tgcgctgcat gcaccgccag cgggtgctcg ggcgcaatgg
     1141 cgcagaacgt cacgcccatg atggtgtcgg gccgggtggt gaacacatac atgcgtccgt
     1201 cgccaatcag ctgcccctgg ctgtcctgga tatcgtgggt aaacgcaaag cgcacaccct
     1261 cgctcttgcc aatccagttc tcctgcatga gcttcacgcg ctccggccag ccgggcaggc
     1321 cgtcctgcac atggcccagc aattcctgtg cgtaatcggt gatcttgagg tagtagcccg
     1381 gtatctcgcg cttctccacc gtcgcacccg tgcgccagcc tttgccgtca atcacctgct
     1441 cattggccag caccgtctgg tccaccgggt cccagttgac gacctgggtc ttgcggtagg
     1501 caatgccttt ttccagcatt ttcaggaaca gccactggtt ccacttgtag tagctggggt
     1561 cgcaggtggc gacctcgcgg ctccagtcga tcgccagccc catggcctgc atctggccct
     1621 tcatgtaggc gatgttctcg taagtccact tggcaggcgg taccttgttc ttcaaggccg
     1681 cgttttcggc aggcaagcca aacgcatccc agcccatggg catcagcacg ttgtagccgt
     1741 tcatgcgcag gtagcgcgtg agcatgtcgt tgatggtgta gttgcgcaca tggcccatgt
     1801 gcagcttgcc gctggggtag ggcagcatgg
//
LOCUS       DAOSJM010000426         1712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_60729_length_1712_cov_3.638503, whole genome shotgun sequence.
ACCESSION   DAOSJM010000426 DAOSJM010000000
VERSION     DAOSJM010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60729_length_1712_cov_3.638503"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..258)
                     /locus_tag="PKX82_12205"
     CDS             complement(55..258)
                     /locus_tag="PKX82_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF53062.1"
                     /translation="MDVSKAKPQQVQQVQATKRAEQVQQTQRREAQVQAEQAQTQKAA
                     ENKPRPNVNGQGQTIGTRLNVTA"
     gene            complement(342..>1712)
                     /locus_tag="PKX82_12210"
     CDS             complement(342..>1712)
                     /locus_tag="PKX82_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOF53063.1"
                     /translation="NTTLVQTVVGSSLSMGLRNLVMGLGALAVLVWTNPVVMLQVLVV
                     LVLIVLPSLWFGRRVRKLSRASQDRVADSSAIAAEVLNAIPVVQSYTAEGRETQRFVT
                     STDNAFATATKRSLMRSVLVAFIIIATSAALLWGLYQGTQAVAEGRISAGHLGQTVVY
                     VIILASAFAVLGEVYGDLLRAAGATERLMELLGTQSPITSPSNPALAPMPAAGSAIKF
                     EAINFHYPSRPQQAALQDFSLDVHPGQTVAIVGPSGAGKSTVFQLLLRYYDPQSGQIV
                     LDGVGTRAMALDALRQRVGIVPQDAVIFSSTALENIRYGKPEATDAEVHAAAHAAFAH
                     EFIQDLPEGYNTFLGERGVRLSGGQRQRIAIARAMLKNPPLLLLDEATSALDAESERM
                     VQAALESAMRDRTTLVIAHRLATVQKADLIVVLDHGRLVEQGTHAELVARGGVYAGLA
                     ALQFNA"
BASE COUNT          286 a          548 c          546 g          332 t
ORIGIN      
        1 gctggtggcg gtgatggcaa ccactgggtg gttcctgtca gaccggcgcg gtgttcaggc
       61 cgtgacgttc aggcgggttc cgatggtctg gccctggccg ttcacattgg gacggggttt
      121 gttctcggcg gctttttgtg tctgtgcctg ttcagcttgc acctgcgctt cgcggcgctg
      181 agtctgctgc acctgctcgg cgcgcttggt ggcttgcacc tgttgtactt gctggggttt
      241 ggctttgctg acgtccatgg aaaatctccc gggagtgaat gccttgattt taggcacata
      301 ccccgcgctt ttcaatgctt tgtcgtgtgc aaaacctgct atcaggcgtt gaattgcaag
      361 gccgccagtc cggcgtacac cccgccacgt gccaccagtt cggcgtgcgt cccttgctcc
      421 accagccggc cgtggtccag taccacgatc aaatcggcct tttgtaccgt ggccagccgg
      481 tgggcaatga ccagcgtggt gcggtcgcgc atggccgact ccagtgccgc ctgcaccatg
      541 cgttcgctct cggcgtccag cgcgctggtg gcttcgtcca gcagcagcag gggcgggttt
      601 ttgagcatgg cgcgtgcaat ggcgatgcgc tggcgctggc cacccgacag gcgcacccca
      661 cgctcgccca ggaaggtgtt gtagccctcg ggcaagtcct ggatgaattc gtgcgcaaag
      721 gcggcatggg cggcggcgtg cacctcggca tcggtggcct cgggtttgcc atagcggatg
      781 ttctccagtg ccgtgctgga aaaaatcacg gcgtcctgcg gcacaatgcc cacgcgctgg
      841 cgcagcgcgt ccaaagccat ggcgcgtgta cccacaccat cgagcacgat ctggccgctc
      901 tgcgggtcgt agtaacgcag cagcagctgg aacaccgtgc tcttgcccgc accgctcggt
      961 cccacgatgg ccacggtctg cccgggatgc acatcgaggc tgaagtcctg caatgccgcc
     1021 tgctgcgggc gggatgggta gtgaaaattg attgcttcga atttgatagc gcttcccgca
     1081 gcaggcatgg gggctagagc cggatttgag ggtgaagtga tgggagactg ggtgcctagc
     1141 agctccatca gccgctcggt ggcacccgct gcgcgcagca ggtcgccata cacctcgccc
     1201 agcactgcaa aggcgctggc cagaatgatc acgtacacca cggtttgccc gaggtggccc
     1261 gcgctgatgc ggccttcggc aaccgcctgc gtgccctggt acagacccca cagcagcgcg
     1321 gccgacgtgg caatgatgat gaaggccacc agcaccgaac gcatcaggct gcgcttggtg
     1381 gcagtggcaa acgcgttgtc ggtcgaggtg acaaaccgct gcgtctctcg tccctcagcc
     1441 gtgtagctct gcaccacggg aatggcgttg agcacctcgg ccgcaatcgc actggagtcc
     1501 gccacccggt cttgagacgc gcgagagagt ttgcgcaccc gtcggccaaa ccacaggctg
     1561 ggcagcacga tcagcaccag caccaccagc acctgcagca tcaccaccgg gttggtccag
     1621 accagcaccg ccagcgcgcc caatcccatc accaagttgc gcagccccat gctcagcgac
     1681 gagccgacca cggtttgcac cagtgtggtg tt
//
LOCUS       DAOSJM010000427         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_61578_length_1696_cov_4.938452, whole genome shotgun sequence.
ACCESSION   DAOSJM010000427 DAOSJM010000000
VERSION     DAOSJM010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61578_length_1696_cov_4.938452"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKX82_12215"
     CDS             <1..569
                     /locus_tag="PKX82_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HOF53064.1"
                     /translation="DIGMPSGHAYAKALRTVKTCVGSEWCRMGTQDSTQMGKDLERAH
                     WRMYAPHKVKYAVSGCPRNCAEAGIKDVGIIGVDSGWEMHIAGNGGIKTEVAHFFAKL
                     KTAEEVLEYTGAFMQLYRQEGWYLERTVHYVNRVGLEYVKKRILEDAAGRKALNEALI
                     FALDGEPDPWFEFDKAAVDTRQFEAITA"
     gene            628..1011
                     /gene="nirD"
                     /locus_tag="PKX82_12220"
     CDS             628..1011
                     /gene="nirD"
                     /locus_tag="PKX82_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HOF53065.1"
                     /translation="MTEWKKICLVTDIPVLGSRRVTRAQGLDVAVFRNDKDEVFALLD
                     RCPHKGGPLSQGIVFGTHVACPLHNWQIGLCDGQASAPDEGCTPKFSLKLEEGMVYLK
                     TAELENHATDLVRPIAGPARRTATA"
     gene            1008..>1696
                     /locus_tag="PKX82_12225"
     CDS             1008..>1696
                     /locus_tag="PKX82_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOF53066.1"
                     /translation="MTETRSTCPYCGVGCGVIITSDGAQITDVRGDPDHPANFGRLCT
                     KGSTLHLTASSAVTQQTRLLQPLRRMERGGVPQAVSWEQALDATAQSLQRIVQAHGPD
                     AVGLYVSGQLLTEDYYAFNKLAKGLLGTNNIDTNSRLCMSSAVAGYKQTLGADAPPAC
                     YDDVNHAQCIFIVGSNTAFAHPILFRRIEDAKKANPALKIIFCDPRRTDTAGIADLFL
                     PIQPGTDVMLF"
BASE COUNT          353 a          570 c          508 g          265 t
ORIGIN      
        1 aagacatcgg catgcccagc ggccacgcct acgccaaggc gctgcgcacg gtgaaaacct
       61 gcgtcggcag cgaatggtgc cgcatgggca cgcaggacag cacccagatg ggcaaggacc
      121 tggagcgcgc ccactggcgc atgtatgcac cgcacaaggt caagtacgcc gtctccggct
      181 gcccgcgcaa ctgcgcggaa gccggcatca aggacgtggg catcatcggc gtggacagcg
      241 gctgggaaat gcacatcgcc ggcaacggcg gcatcaagac cgaggtggcg cacttctttg
      301 ccaagctcaa aaccgccgag gaagtgctgg agtacaccgg cgccttcatg cagctctacc
      361 gccaggaagg ctggtacctg gaacgcacgg tgcactacgt gaaccgcgtg ggtctggagt
      421 atgtgaagaa gcgcattctg gaagacgctg ccggccgcaa ggccctgaac gaagcgctga
      481 tcttcgcgct ggatggcgag cccgatccct ggtttgaatt cgacaaggcg gcagtggata
      541 cgcgccagtt cgaagccatc actgcataac aaatagccct ttagcccccg aataataagc
      601 acatacagct atgaaaggta tagcaccatg accgaatgga agaagatttg tctcgtcacc
      661 gacatccccg tactgggttc gcgtcgcgtg acccgcgcgc agggactgga cgtggcggtg
      721 ttccgcaacg acaaggatga agtgtttgcg ctgctggacc gctgcccgca caagggcggc
      781 cctttgagcc agggcattgt gtttggcacc catgtggcct gcccgctgca caactggcaa
      841 atcggtctgt gcgacggaca ggccagcgcg cccgatgaag gctgcacgcc caagttcagc
      901 ctcaagctcg aagagggcat ggtctatctc aaaaccgcag aactggaaaa ccacgccacc
      961 gatctggtgc gccccatcgc cggccccgcc cgccgcactg ccaccgcatg acagaaacgc
     1021 gctccacctg cccctactgc ggcgtgggct gcggcgtcat cattacctcg gacggcgcgc
     1081 agatcaccga tgtgcgcggc gacccggacc acccggccaa cttcgggcgt ctgtgcacca
     1141 agggcagcac cctgcacctg acggccagca gcgcggtcac gcagcaaacc cgcctgctgc
     1201 aaccgctacg gcgcatggag cgcggcggtg tgccccaggc cgtatcctgg gaacaggcgc
     1261 tggacgctac cgcccagtcc ctgcagcgca tcgtgcaggc gcacggcccg gatgccgtgg
     1321 gcctgtatgt ctcgggccag ttgcttaccg aagactatta cgccttcaac aagctcgcca
     1381 agggcctgct gggcaccaac aacatcgaca ccaattcgcg cctgtgcatg agcagcgccg
     1441 tggcgggcta caagcagacg ctgggcgccg acgcgccccc ggcctgctac gacgacgtga
     1501 accatgcgca gtgcattttc atcgtcggct cgaacaccgc gttcgcccac cccatcctgt
     1561 ttcgccggat tgaagacgcc aaaaaggcca accccgcact caagatcatc ttctgcgatc
     1621 cgcgccgcac cgatacggcc ggcattgccg acctgttcct gccgatccag ccgggcaccg
     1681 atgtcatgct gttcaa
//
LOCUS       DAOSJM010000428         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_62279_length_1683_cov_4.154791, whole genome shotgun sequence.
ACCESSION   DAOSJM010000428 DAOSJM010000000
VERSION     DAOSJM010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62279_length_1683_cov_4.154791"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PKX82_12230"
     CDS             complement(<1..345)
                     /locus_tag="PKX82_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOF53067.1"
                     /translation="MSDELKSPQPGTEPPSVPLMPLGKVVAVTSGKGGVGKTFFSANM
                     AASLAKRGLRVLVLDADLGLANLDVVLNLYPKITLHDVFTGTAKLEEAVIKAPGGFSV
                     LLAGSGMVEYSRL"
     gene            complement(345..1136)
                     /locus_tag="PKX82_12235"
     CDS             complement(345..1136)
                     /locus_tag="PKX82_12235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOF53068.1"
                     /translation="MAATTPASLVELQQLRLDDARALLGPEMVEALPPREQSAAYLQG
                     VIDGLCELSLKDPLTGLSNRRHFRNVMERTIDIVARSGDPALLLMLDVDHFKNVNDTY
                     GHHAGDLVLQAVGRTLSKCVRPMDTVARYGGEEFAAILPNCHSHYGETVAERIRQAIE
                     KLTIQIAPGIQIGITISIGGAYAPEWVRSTTALWTERADTQLYNSKTGGRNRVSLDQP
                     QEIYVSAEEKNMLFGHLALGDPAWIESVSGDTPTSNANGSAQRVN"
     gene            complement(1196..>1683)
                     /locus_tag="PKX82_12240"
     CDS             complement(1196..>1683)
                     /locus_tag="PKX82_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HOF53069.1"
                     /translation="APRIYWGVLAGVMLGLIDFLYLRLHPRIIEVGLHPDGSLRDRHL
                     WKLAPLAPHMYALRMDAELDFAASSSFERNIADFLSAHPDTRHVCLFAHPINRIDASG
                     VEVFAALSTNLQERGIALHISGIKLPVETVLRRSGLLTPHPLLQLYRTDAEALECIAR
                     L"
BASE COUNT          333 a          498 c          527 g          325 t
ORIGIN      
        1 caggcgtgaa tactccacca tgccggagcc cgccagcagc accgaaaacc cgcccggcgc
       61 cttgatcacc gcttcttcga gcttggctgt gccggtaaac acatcgtgca gcgtaatctt
      121 gggatacagg ttcagcacca catccagatt ggccaggccc aggtcggcat ccagcaccag
      181 cacccgcagc ccacgcttgg ccaacgacgc tgccatgttg gcagagaaaa acgttttgcc
      241 gacgccaccc ttgccgctgg tcacggccac caccttgccc agaggcatca gtggaactga
      301 agggggctca gtaccgggtt ggggggattt cagctcgtcc gacatcaatt aaccctttgc
      361 gcactgccat tggcattgct ggttggcgta tcgccagaaa cactctcgat ccaggcggga
      421 tcgcccagcg cgagatgacc gaacagcatg ttcttttcct cggcgctgac ataaatttct
      481 tgtggctggt ccagcgagac gcggttgcgc ccgccggtct tggagttgta gagctgcgta
      541 tccgcccgct cggtccacag tgccgtggtg gagcgcaccc actcgggcgc ataggctccg
      601 ccgatactga tggtgatacc gatctggatc ccgggcgcaa tctggatggt cagcttttcg
      661 atagcctggc ggatgcgctc tgccacggtt tcaccgtagt gcgaatggca gttgggcaga
      721 atggccgcaa actcctcacc tccgtagcgt gcgacggtat ccatagggcg tacgcacttg
      781 gacagtgtgc gccccaccgc ctgcaggacc aggtcaccgg catggtgacc gtaggtgtcg
      841 ttcacgttct tgaaatggtc gacatcgagc atcagcaaca gtgcggggtc acccgagcgc
      901 gccacgatgt cgatcgtgcg ctccatgacg ttgcggaagt ggcgccggtt ggacagaccg
      961 gtcagcgggt ccttgagcga cagttcgcac aggccatcta taacgccctg caagtaggcg
     1021 gcggactgtt cgcgaggggg cagggcctca accatctccg ggcccagcag ggcacgtgca
     1081 tcatccaatc gcagttgctg caattcgacc agagatgcgg gtgttgtggc tgccacgttg
     1141 gatgttgctt gggattgcgt tcggcgttcg gcgaaagtct aacaggaagc cgtgcctata
     1201 ggcgcgcaat gcattccagt gcttctgcat cggtgcggta aagctgcaac agcggatgcg
     1261 gtgtgagcag acccgagcgc cgcagcacgg tttccaccgg gagtttgatg ccgctgatgt
     1321 gcagtgcgat gccgcgttcc tgcagattgg tagacagtgc agcaaaaact tctacgccac
     1381 tggcatcaat acggttgata gggtgtgcga acaggcagac gtggcgcgtg tcggggtgtg
     1441 cactgaggaa gtcggcaata ttgcgctcaa aactgctgga cgcagcgaag tccagttccg
     1501 catccatgcg cagggcatac atgtgcggtg ccagcggtgc cagcttccac aaatgccggt
     1561 cgcgcagact gccatccgga tgcagcccca cttcgatgat gcgcgggtgc aggcgcaggt
     1621 agaggaagtc gatcaggccc agcatgacgc ctgccagaac gccccagtaa atgcggggag
     1681 cgg
//
LOCUS       DAOSJM010000429         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_62897_length_1672_cov_2.714904, whole genome shotgun sequence.
ACCESSION   DAOSJM010000429 DAOSJM010000000
VERSION     DAOSJM010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62897_length_1672_cov_2.714904"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PKX82_12245"
     CDS             complement(<1..597)
                     /locus_tag="PKX82_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HOF53070.1"
                     /translation="MSTGAATGGPLAARILDALPQTQCTRCGYPDCAGYAEAIAQGQA
                     DINQCPPGGAEGVARLAALTGKPIQALNPEFGNESPRSVVFIDEAWCIGCTLCIAACP
                     TDAIVGSNKRMHTVLEAYCTGCELCLPVCPVDCIVVENASGAATGWDAWSPTLASLAR
                     KRYEFNSYRRKRDEGEHAKRLEEKALMKLADLPAHSQHT"
     gene            complement(594..>1672)
                     /gene="phaZ"
                     /locus_tag="PKX82_12250"
     CDS             complement(594..>1672)
                     /gene="phaZ"
                     /locus_tag="PKX82_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HOF53071.1"
                     /translation="LLRDTVKTMLKDHKVYITDWKNARLVPLEDGEFHLDDYVNHVQE
                     FIRHVQKLYGNCHVMSVCQPTVPVLAAVSLMASRGELTPLSMTMMGGPIDARKSPTAV
                     NNLAMNKSHSWFENNVIYRVPSSFPGAGRRVYPGFLQHTGFVAMNPDHHAKSHYDYFK
                     DLIKGDDASTEAHRKFYDEYNAVLDMDADYYLETIHVVFQDFSLVSGTWDVKNPQGKI
                     ERVRPADIQTTALLSVEGELDDISGSGQTQAVHDICSGVPRSQQVHFEAKGAGHYGIF
                     SGRRWREHVYPVVKAFILSHHNKTPATATPAASKSVETKAVAVQKVAAKAPARKAPAA
                     PRAVAKPAKAVVKKPATKRVAAKK"
BASE COUNT          264 a          491 c          546 g          371 t
ORIGIN      
        1 ggtgtgctgg gagtgtgcag gcaggtctgc cagcttcatc aaagcctttt cttcaagcct
       61 ttttgcgtgt tcgccctcgt cgcgcttgcg ccggtagcta ttgaattcat agcgttttct
      121 ggccagagat gcgagtgtgg gtgaccaggc gtcccatccg gtggcggctc cgctggcgtt
      181 ttccaccacg atgcaatcca ccgggcatac cggcaggcac agttcacagc ccgtgcaata
      241 ggcttccagc accgtgtgca tgcgtttgtt gctgcccaca atggcatcgg tcgggcaggc
      301 cgcgatgcac agcgtgcagc caatgcacca ggcctcgtcg atgaacacca cgctgcgggg
      361 tgattcgttg ccaaattcgg ggttgagggc ctggatgggc tttcccgtca gcgcagcgag
      421 ccgggcaacg ccttcggcgc cgcctggcgg gcactggttg atatcggctt gtccctgtgc
      481 aatggcctcg gcataacccg cgcaatcggg ataaccgcag cgtgtgcact gggtctgggg
      541 cagcgcatca agaatccggg cggccagtgg gccacccgtg gctgcccccg tgctcacttc
      601 ttcgcggcta cccgtttggt ggccggcttc ttcaccactg ccttggctgg cttggctact
      661 gcccgtggcg cagcgggtgc tttgcgggct ggggccttgg cagccacctt ttgcactgca
      721 actgccttgg tttccacgga cttgctggcc gcgggcgtgg cggttgctgg agtcttgttg
      781 tggtgcgaca ggataaatgc cttgaccacg gggtacacat gctcgcgcca gcggcggccg
      841 gagaagatgc cgtagtggcc tgcgcccttg gcttcgaaat gcacttgctg tgatctgggc
      901 acgccgctgc agatgtcgtg cacggcctgg gtctggcccg agccggaaat atcgtccagt
      961 tcgccctcga ccgacagcag ggcagtggtc tggatgtcgg ccggacgcac gcgctcaatc
     1021 ttgccctgcg ggttcttgac atcccaggta ccgctcacca ggctgaagtc ctggaacacc
     1081 acgtggatgg tttccaggta gtaatcggca tccatgtcca gtacggcgtt gtactcgtcg
     1141 tagaacttgc ggtgggcttc ggtgctggca tcgtcgccct tgatcaggtc cttgaagtag
     1201 tcgtagtggc tcttggcgtg gtggtccggg ttcatggcca cgaaaccggt gtgctgcagg
     1261 aagccggggt acacgcggcg gccagcgccg gggaagctgc tggggacgcg gtaaatcacg
     1321 ttgttttcga accagctgtg gctcttgttc atggccaggt tattcaccgc ggtgggcgac
     1381 ttgcgggcgt caatcgggcc acccatcatg gtcatggaca acggggtcaa ttcgccgcgg
     1441 ctggccatca gggacacggc agccagcacc ggtaccgtag gctggcacac gctcatgacg
     1501 tggcagttgc cgtacagctt ctgcacgtgg cggatgaatt cctgcacatg gttgacatag
     1561 tcatccaagt gaaattcgcc atcttccagc ggcaccaggc gggcgttttt ccagtcggtg
     1621 atgtagacct tgtggtcctt cagcatggtc ttgacggtat cgcgcagcag cg
//
LOCUS       DAOSJM010000430         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_64218_length_1647_cov_4.329774, whole genome shotgun sequence.
ACCESSION   DAOSJM010000430 DAOSJM010000000
VERSION     DAOSJM010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64218_length_1647_cov_4.329774"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PKX82_12255"
     CDS             <1..210
                     /locus_tag="PKX82_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOF53072.1"
                     /translation="PRYLLLDEATSALDATNEGLLYGQLAGLSITPISVSHHEAIVRH
                     HRHVLEMPGDGRWSLHDARDYRWVA"
     gene            complement(228..1346)
                     /locus_tag="PKX82_12260"
     CDS             complement(228..1346)
                     /locus_tag="PKX82_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOF53073.1"
                     /translation="MATSTPVAATPMAFVQAILLAYARYGKDPAQALQQAQIAPEDIP
                     RPRGRITAWQMERLSEAAMRELDDEALGWFSRRLPWGSYGMLARASISAPTLHLALSR
                     WCRHHGLIAPDISLALDVKDDVATIRITEHPATRGGCAAGDSAQVKEEPAARAGDTEQ
                     SPLPHNLPEPMREFCLVSVLRNILGVACWLVDSRIPLQAAQFPFDAPAHQSAYAILFS
                     GPTTFSADHAAIHFDARYLNLPLRRDEKALQQMLQHALPLTVLQYRRDRLLVQRVRQT
                     LATHAQQTHSAEALAALLHVSPRTLHRQLKEEGATLQGLKDEVRQARAIELLNRTDRP
                     IKQVAEAAGFQNEKSFIRAFRGWTGQSPAEFRRQSVPA"
     gene            1468..>1647
                     /locus_tag="PKX82_12265"
     CDS             1468..>1647
                     /locus_tag="PKX82_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylcrotonoyl-CoA carboxylase"
                     /protein_id="HOF53074.1"
                     /translation="MPILETKLNARSADFQANATAMRALVADLNAQIDKAALGGGEAA
                     RAKHTARGKLLPRDRV"
BASE COUNT          275 a          525 c          555 g          292 t
ORIGIN      
        1 ccccgctacc tgttgctcga cgaagccacc agcgcgctgg atgccaccaa tgaaggcctg
       61 ctgtatggcc agctggcagg cctgtccatc acgcccatca gtgtcagcca ccacgaagcc
      121 atcgtccgcc accaccgcca tgtgctcgaa atgcccggcg acggacgctg gtcgctgcac
      181 gatgcgcggg actaccgctg ggtggcttag cgcggcccgc gcggttgcta tgccggcacc
      241 gactggcgcc ggaactccgc gggggattgt ccggtccagc ctctgaaggc acggataaag
      301 ctcttctcgt tctggaatcc tgccgcctcg gccacctgct tgatggggcg gtcagtgcgg
      361 ttcaatagct cgatggcccg tgcctgtcgc acctcgtcct tgagcccctg cagcgtggcg
      421 ccttcctcct tgagttggcg gtgcagggtg cgcggagaga catgcagcag cgcggccagg
      481 gcctcggcgc tatgggtctg ctgggcgtgg gttgccaggg tctggcgcac acgctgcacc
      541 agcaaacggt cgcggcggta ttgcagtacg gtcagcggca gggcgtgttg cagcatctgc
      601 tgcagcgcct tttcgtcgcg acgcaagggc aggttcaggt agcgggcatc aaagtggatg
      661 gcggcgtggt ctgcactaaa cgtggtcggg ccggagaaca ggatggcata cgcgctctgg
      721 tgcgccggtg cgtcaaaggg gaactgcgcg gcctgcaatg ggatgcgcga atccaccagc
      781 cagcatgcca cccccaggat gttgcgcagt accgagacca ggcagaactc gcgcataggt
      841 tctgggaggt tgtgcggcag gggactctgc tccgtgtccc ccgcccgcgc tgcgggctcc
      901 tcctttacct gcgcagaatc ccctgccgca cagcctcccc gagttgcagg gtgctccgtg
      961 atgcggattg tcgccacatc gtctttcacg tccagtgcga gtgagatgtc gggtgcaatc
     1021 aacccgtggt ggcggcacca gcgtgacagg gccaggtgca aggttggtgc gctgatggac
     1081 gcacgcgcca gcatgccata gctgccccag ggcaggcgac ggctgaacca gcccagcgct
     1141 tcgtcatcga gttcgcgcat cgcggcctcg gacaggcgct ccatctgcca tgccgtgatg
     1201 cgtccgcggg ggcgggggat gtcttctggc gcaatctgtg cctgttgtag tgcctgggcc
     1261 gggtctttgc catagcgcgc gtaagccagc agaatcgctt ggacgaaggc catgggcgtt
     1321 gcggcgacgg gggtggaggt ggccatggcg tcggagtgtg ccagaaattc agggtctggc
     1381 acgatttgca accttgatgg cctgcccgga tggcctctgc ccgttcaaaa tgcggggtac
     1441 accgaaaaca tctaccggac acagaccatg cccattctgg agaccaaact caacgcccga
     1501 tctgccgact tccaggccaa cgccactgcc atgcgtgcgc tggtggcaga cctgaatgcc
     1561 caaatcgaca aggcggcact cggtggtggc gaggcagcgc gcgccaagca caccgcgcgc
     1621 ggcaagctgc tgccccgcga ccgcgtg
//
LOCUS       DAOSJM010000431         1629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_65190_length_1629_cov_3.981576, whole genome shotgun sequence.
ACCESSION   DAOSJM010000431 DAOSJM010000000
VERSION     DAOSJM010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65190_length_1629_cov_3.981576"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..754)
                     /locus_tag="PKX82_12270"
     CDS             complement(191..754)
                     /locus_tag="PKX82_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOF53075.1"
                     /translation="MSSTRPSTAIPGFRSPGVGFEAPFDMLEACHERVERTLNLLLRL
                     QQHVLTHGCDTQAVQAAQDILRYFDLAAPLHHQDEELHVFPLLLAGNNAVARDAVHRL
                     MAQHRQMEGAWGAMRLVLLDMAEPAEAPPPRSLSDAEVRAFVDLYRDHMRLEEEVAYP
                     AARTVMDAEQLRSAGAEMQSRRRVAGP"
     gene            complement(770..1174)
                     /locus_tag="PKX82_12275"
     CDS             complement(770..1174)
                     /locus_tag="PKX82_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase subunit alpha"
                     /protein_id="HOF53076.1"
                     /translation="MNIENFNDLLQAARSQPLPQRLLFVFVGVELPEDSTPEQRAQFE
                     AGHGGALVPLMCVDKSPDELASFDVLKAEASQFGKSWALVFAAAMSGSVLAPPSSADA
                     DKPLQAMVEAIKRGDIAAYIPFDAQGQAVHLG"
     gene            complement(1171..>1629)
                     /locus_tag="PKX82_12280"
     CDS             complement(1171..>1629)
                     /locus_tag="PKX82_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOF53077.1"
                     /translation="ATARGLLADQFASALVERDTEALAEQVEQAREKAYRAPLLLLVV
                     VDGARGDASVDLHERIVSVGCAVQNMLLMATSLGYGSALTSGKALKSQGLRSLFALAA
                     QEHALCFVSVGTVQSRKPQRSRPTPADYVAHLTVAQGVVSGLPLQELPTE"
BASE COUNT          351 a          520 c          458 g          300 t
ORIGIN      
        1 aggccaaagc ctgcacccat tcgaaccccg attttcaagt cattgaatgc cacggaagtt
       61 ccctcctcaa ttaaattgga ctgctcgcat gcgtctcact atgccgaaaa gtaagcctgg
      121 cgacaatcct acctctggac gcacgcgcac cgccaggttt ctatttccct actaccaggg
      181 tagtaattat tcaggggcca gcaactctgc gacgggactg catctcggcg cccgctgacc
      241 ggagttgctc ggcatccatc accgtgcgcg cagcaggata ggccacttct tcctccagac
      301 gcatgtggtc tcggtacaga tccacgaatg cgcgaacctc agcgtccgac aatgaccgtg
      361 gggggggggc ttcagcgggt tccgccatgt ccagcaatac caggcgcatc gccccccaag
      421 cgccctccat ctggcgatgc tgcgccatga gtcggtgtac cgcatcgcgg gctacagcat
      481 tgttccccgc aagcaatagc ggaaatacat gcaactcttc gtcctggtga tgcaaagggg
      541 cagccaaatc aaaatagcgc aggatgtcct gcgctgcctg caccgcctgg gtatcgcacc
      601 catgggtgag cacgtgctgc tgcaaacgca gcagcaggtt cagggtgcgt tctacgcgct
      661 cgtggcatgc ctcgagcatg tcaaaaggcg cttcgaaacc gacaccgggc gaccggaaac
      721 ccgggattgc ggtcgatggg cgagtggaag acatggacac aggccccact caacccagat
      781 ggactgcctg tccctgggca tcaaaaggga tgtaggcagc aatgtctccc cgcttgatgg
      841 cttccaccat ggcctgcaat ggcttgtctg catctgcgct ggacggtggc gccagcactg
      901 accccgacat ggcggccgca aaaaccagcg cccagctctt gccaaattga ctggcctccg
      961 ctttgaggac atcaaaggat gccagctcat cgggcgattt gtcgacgcac atcaaaggca
     1021 ccagggcgcc tccatgccca gcctcgaatt gggcgcgctg ctcgggtgtg ctgtcttccg
     1081 gcaactccac gccgacaaac acaaacaaaa gccgctgggg caaaggctgg ctgcgtgccg
     1141 cctgcaaaag atcgttgaaa ttttcgatgt tcattctgta ggtaattcct gtaatggcaa
     1201 acccgacacc actccctgag ccacggtcag atgggccaca tagtcggcag gagtcggacg
     1261 ggaacgttgg ggcttgcggg actggacggt ccccacactg acgaagcaca gcgcatgctc
     1321 ctgcgcagcc agcgcaaaca gactgcgcaa cccttgggat ttgagtgcct tgccgctggt
     1381 gagcgcggaa ccgtagccga gcgacgttgc catcagcagc atattttgaa ccgcacatcc
     1441 cacagagacg atacgttcat gcagatcgac ggaagcatcg cctcgggcac catccaccac
     1501 gaccagcagc aaaagcggcg cgcggtacgc cttttcccgg gcctgctcca cctgttccgc
     1561 cagtgcttcg gtgtcgcgtt ccaccagggc actggcaaac tggtccgcca gcagtccgcg
     1621 cgcggtagc
//
LOCUS       DAOSJM010000432         1612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_66166_length_1612_cov_4.676943, whole genome shotgun sequence.
ACCESSION   DAOSJM010000432 DAOSJM010000000
VERSION     DAOSJM010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66166_length_1612_cov_4.676943"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PKX82_12285"
     CDS             <1..238
                     /locus_tag="PKX82_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF53078.1"
                     /translation="ICSEIFLKVAQFSTGINTRPVYTKFGTPSTPEQANMPLIYTPYV
                     PSLWEDFILRNRRILADQMEFLMAHVPKSSRLLM"
     gene            complement(239..>1612)
                     /locus_tag="PKX82_12290"
     CDS             complement(239..>1612)
                     /locus_tag="PKX82_12290"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOF53079.1"
                     /translation="SFYQNQQLLQQTPPPLSTLAKPDIEGARLVIESVLAERRKVLTE
                     MESKTLLSAFHIPVTRTILARSANEAMMIATQLGFPVALKIDSPDISHKSDVQGVALN
                     IMNATGVRDTYNDMVQTVARLQPQARINGVTVQTMARTRRGREIYIGLVTDDPFGPVI
                     AFGAGGTMIELINDRAMELPPLNQFLANRLINRSRVAETLGEWRGNSPANVDALEQIL
                     LRVSEMVCELPQLREMDINPIIIDENGAVAVDARIVIDHAARGVSNRTDNYNHLSILP
                     YPAHYEQVWPLPGGGEYTVRPIQPDDAQMLQDLMHHLSPESRYFRFVSSIAELPPSML
                     ARFTLIDYDREMALVAIHKERTAGADGEIAETERIVGVSRYITNPDQSSCEFSLVVAD
                     DFNGKGLGSRLMQSIMDVARDKGLTEMDGLVLANNPGMLKLMRSLGFVVKTFDEDPDF
                     KLVTHPL"
BASE COUNT          286 a          491 c          497 g          338 t
ORIGIN      
        1 aatttgcagc gaaattttcc tcaaagtggc tcagttttca accggaatca acactcgccc
       61 cgtttacaca aaattcggga caccctccac ccccgagcag gccaacatgc cgctgatcta
      121 tacgccctac gtgcccagcc tgtgggaaga cttcattttg cgcaaccgcc gtatcctggc
      181 cgaccagatg gagtttctga tggcccatgt gccgaagtcc tcccggctgc tgatgtagtt
      241 acagtgggtg cgtcaccagc ttgaagtccg gatcttcgtc gaaggtcttg acgacaaagc
      301 ccaggctgcg catgagcttg agcatgccgg ggttgttcgc cagcaccaga ccatccatct
      361 ccgtcaggcc cttgtcgcgc gccacgtcca tgatgctctg catcaggcgt gagcccagtc
      421 ccttgccatt gaagtcgtct gcgaccacca gcgagaactc gcagctggac tggtccgggt
      481 tggtgatata gcgcgacaca cccacgatgc gttcggtctc ggctatctcg ccatcggcgc
      541 ctgcagtgcg ttccttgtgg atggccacca gcgccatttc ccggtcgtag tcaatcagcg
      601 tgaagcgcgc cagcatgctg ggtggcagtt ccgcaatact ggagacaaac cggaaatagc
      661 ggctctcggg cgacaggtgg tgcatcaggt cctgcagcat ctgggcatcg tcgggctgga
      721 tcgggcgcac cgtgtattcg ccgccaccgg gcagtggcca tacctgctcg tagtgggccg
      781 ggtagggcag gatggagagg tggttgtagt tgtccgtgcg gttgctcacg ccacgtgccg
      841 cgtggtcgat cacgatgcgc gcatccacgg ccaccgcacc gttctcgtca atgatgatgg
      901 ggttgatgtc catctcgcgc agttgcggca gttcacacac catttccgag acgcgcaaca
      961 ggatctgctc cagcgcgtcc acattggccg ggctgttgcc gcgccattcg cccagagttt
     1021 cagccacgcg cgagcggttg atcaggcgat tggccagaaa ctggttcagc ggtggcagct
     1081 ccatggcccg gtcgttgatc agttcgatca tggtgccgcc cgcaccaaac gcaatcaccg
     1141 gaccaaacgg gtcgtcggtc accaggccga tatagatctc gcggccacgg cgtgtgcgcg
     1201 ccatggtctg taccgtcacg ccgttgatgc gtgcctgggg ctgcagccgg gccacggtct
     1261 gcaccatgtc gttgtaggtg tcacgcacgc cggtggcgtt catgatgttg agggccacgc
     1321 cctgcacgtc cgacttgtgg ctgatatcgg gggagtcgat cttcagtgcc accgggaagc
     1381 ccagctgggt ggcgatcatc atggcttcat tggcactgcg ggccaggatg gtgcgcgtca
     1441 ccggaatgtg gaacgccgac agcagcgtct tggactccat ctcggtcagt accttgcggc
     1501 gctctgccag cacactttcg atgacgaggc gggcgccttc aatatcgggc ttggccagtg
     1561 tcgagagcgg tggcggcgtt tgctgcaaca gctgctggtt ctggtagaac ga
//
LOCUS       DAOSJM010000433         1601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_66804_length_1601_cov_4.690168, whole genome shotgun sequence.
ACCESSION   DAOSJM010000433 DAOSJM010000000
VERSION     DAOSJM010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66804_length_1601_cov_4.690168"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PKX82_12295"
     CDS             complement(<1..841)
                     /locus_tag="PKX82_12295"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOF53080.1"
                     /translation="MKTELTTLDTTRIDDTRIGAVRPLITPALLEEKLPVPTAAQALV
                     ESSRAAISDVLHGKDDRLVVVVGPCSIHDHEQAMDYAWQLKEQADALKDDLLVVMRVY
                     FEKPRTTVGWKGYINDPHLDGSFAINEGLEMARKLLLDILAIDMPVGTEFLDLLSPQF
                     ISELVSWGAIGARTTESQSHRQLASGMSCPVGFKNGTDGGIKVATDAIQAAQAAHAFI
                     GMTKMGQAAIFETRGNDDCHVILRGGKTTNYSKADVDAACALLKAAGLREQVMIDVSH
                     ANSS"
     gene            complement(859..>1601)
                     /locus_tag="PKX82_12300"
     CDS             complement(859..>1601)
                     /locus_tag="PKX82_12300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOF53081.1"
                     /translation="DHRDTGQSSTVDFQSAPYDMDTLTDDAVAVIHGHRLVRPTVVGL
                     SMGGYIAQLMAVRHPGIASRLVLISSTADHRPYMAATMGQGHSAFTLPKPERIYLDYL
                     LSAAIHPPRTHAETLQSMVDGWAVTYGGSRPFPRAEVTHAIGLAMQRSSSLSAGFHHA
                     LAVAASPHRLELVLRIAVPTLVIHGRHDPLLPLPHGNYLAHHIPLAQLEVLDMGHAFI
                     WSWDAEVLSLVQGFISQNPTQPPRNVRE"
BASE COUNT          275 a          472 c          524 g          330 t
ORIGIN      
        1 tgctgctgtt ggcgtggctc acgtcaatca tcacctgctc gcgcaggccg gcggctttaa
       61 gcagggcgca ggccgcatcc acatcggcct tgctgtagtt ggtggttttg ccgccgcgca
      121 ggatcacatg acagtcgtcg ttgccgcgcg tctcgaagat ggccgcctgg cccatcttgg
      181 tcatgccgat gaaagcatgc gctgcctgtg ccgcctggat cgcatcggta gcgaccttga
      241 tgccaccatc ggtgccgttc ttgaagccta ccgggcagct catgccgctg gccaactggc
      301 ggtggctctg gctttcggtg gtgcgtgcgc caatggcgcc ccagctcacc agttcgctga
      361 tgaactgggg ggaaagcaaa tccagaaact cggtgcccac cggcatgtcg atggccagga
      421 tgtccagcag cagcttgcgc gccatctcca gcccttcatt gatggcaaag ctgccgtcca
      481 ggtgcgggtc gttgatatag cctttccagc ccacggtagt gcggggtttc tcgaagtaca
      541 cgcgcatcac caccagcagg tcgtccttca aggcatcggc ctgctccttg agttgccagg
      601 cgtagtccat ggcctgttcg tggtcgtgaa tggagcaggg tcccaccacc accaccagcc
      661 ggtcgtcctt gccatgcagc acatcggaga tggctgcgcg gctgctctcc accagcgcct
      721 gcgcggcggt gggcaccggc aacttctctt ccagcagcgc gggcgtgatg agaggacgta
      781 ccgcgccaat acgggtgtcg tcaatgcggg tggtgtcgag ggtggtgagt tcggttttca
      841 ttgctattaa ttgtatagct actcgcgcac attccgcggg ggctgggtcg ggttttggct
      901 tatgaaccct tgcaccaggc tcagaacttc ggcatcccac gaccatatga aggcatgtcc
      961 catgtccagc acctccagct gggcgagagg aatgtggtgt gccagatagt tgccatgggg
     1021 caagggaagc aaggggtcgt ggcgcccatg gattaccagt gtaggtactg caatgcgaag
     1081 tacaagttcc agccggtggg gcgaggcagc caccgccagc gcgtggtgaa agcctgcgct
     1141 gaggctggat gaccgctgca tagccagccc tatcgcgtgt gtgacttctg cccgcgggaa
     1201 agggcggctg ccgccgtacg tcacggccca gccgtccacc atgctttgca gcgtttccgc
     1261 gtgcgtgcgg ggtgggtgta tggcggctga caaaaggtaa tcgaggtaga ttctctcggg
     1321 ttttggaagg gtaaaagcag agtggccttg ccccatggtc gccgccatgt aggggcggtg
     1381 gtctgccgtg ctggagatca gcaccagacg gctggcgata ccggggtgcc gaactgccat
     1441 cagttgcgca atgtatccac ccatggacag gcccaccacg gtgggccgca ccagccggtg
     1501 gccatggatg accgccacgg catcgtctgt cagcgtatcc atgtcgtacg gggcgctttg
     1561 gaaatccacc gtggacgatt gcccggtgtc gcggtggtcg t
//
LOCUS       DAOSJM010000434         1582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_67968_length_1582_cov_4.760969, whole genome shotgun sequence.
ACCESSION   DAOSJM010000434 DAOSJM010000000
VERSION     DAOSJM010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67968_length_1582_cov_4.760969"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PKX82_12305"
     CDS             complement(<1..301)
                     /locus_tag="PKX82_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOF53082.1"
                     /translation="MAFAQLQLAAPIARAVAEMGYESMTPIQAQAIPVVLTGQDVMGA
                     AQTGTGKTAAFALPLLQRMMKHENASTSPARHPVRALVLLPTRELADQVAQQVKLY"
     gene            complement(466..1374)
                     /locus_tag="PKX82_12310"
     CDS             complement(466..1374)
                     /locus_tag="PKX82_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOF53083.1"
                     /translation="MKWEGNRESDNVEDRRSSGGAGGGFSGGGLGGLLGALLGSKLGV
                     GTIVIALLGSWALGINPLTILSLLSGEGAPTAQVQQAPAQRPPADDQMARFVSTVLAD
                     TEDVWTDVFSQGGGTYQQPKLVLFRGATATSCGQGQSAMGPFYCPADRKVYIDLGFYQ
                     TLQKQLGAPGDFAQAYVIAHEVGHHVQNLLGVSAKVEQARGRMSEAEYNALSVRVELQ
                     ADCLAGVWAHHANNARQLLEQGDVEEAMNAAARIGDDALQRGSGGAVVPESFTHGSSA
                     QRQKWFATGLKNGTVKGCDTFSSRNL"
     gene            complement(1394..>1582)
                     /gene="dcd"
                     /locus_tag="PKX82_12315"
     CDS             complement(1394..>1582)
                     /gene="dcd"
                     /locus_tag="PKX82_12315"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517930.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOF53084.1"
                     /translation="EWEGYVTLEFSNTTPLPAKIYAGEGCAQVLFFESDKDDVCEVSY
                     KDRGGKYQGQHGVTLPKA"
BASE COUNT          275 a          508 c          505 g          294 t
ORIGIN      
        1 cgtacagctt gacctgctgg gcgacctggt cggccagttc acgggtgggc aacagcacca
       61 gcgcgcgcac cgggtggcgg gcgggggaag tggaagcgtt ttcgtgcttc atcatgcgct
      121 gcagcagggg cagtgcaaag gcagcggttt tgccggtgcc ggtctgggcg gcgcccatga
      181 cgtcctggcc ggtcagcacc acaggaatgg cctgggcctg gatcggggtc atggactcgt
      241 agcccatttc agccacggcg cgggcgatgg gggcggccag ctgcagctgg gcaaaggcca
      301 tgatgggagt gtcggcgttc agctcgttgg aagctgcaga aggcacggca gtggggggag
      361 tcagttcagt attcaaatcg gtccggcata gccaaaagag ttgctatcca tccgatagcg
      421 gggaggcccc agcgtgtagg ggcaaacccg cattatctcc tactcctaca aatttcggct
      481 ggaaaaggta tcgcagccct tgaccgtgcc gtttttcagg ccggtggcaa accatttctg
      541 gcgctgggca ctggaaccat gggtaaagct ctcggggacc accgcgccac cgctgccacg
      601 ctgcaaggcg tcgtcgccaa tccgggcagc ggcgttcatg gcctcttcca catcgccctg
      661 ctccagcagc tggcgggcgt tgttggcatg gtgggcccac acgccggcca ggcagtcagc
      721 ctgcagttcc acacgcacgc tgagggcgtt gtattcggcc tcgctcatac ggccacgggc
      781 ctgctctacc ttggcgctga cgcccagcag gttctgcaca tggtggccca cttcgtgcgc
      841 aatcacgtag gcctgggcaa agtcaccggg ggcacccagc tgcttttgca gggtctggta
      901 aaagcccagg tcgatgtaga ccttgcggtc ggccgggcag tagaacggcc ccatggcgct
      961 ctggccctgc ccgcacgacg tagcagtagc gccgcgaaac aacaccagtt tgggctgctg
     1021 gtaggtgcca ccaccctggc tgaacacatc ggtccagaca tcctcggtat cggccagtac
     1081 ggtagacaca aaccgggcca tctggtcgtc cgcaggtggg cgctgcgccg gcgcctgctg
     1141 cacctgtgcg gtcggcgcac cttcaccact gagcagactc agaatcgtca aggggttgat
     1201 gcccagcgcc cagctgccca gcagcgcgat gacgatggtg cctaccccca gcttgctgcc
     1261 cagcagggcc ccgagcaagc cccccaggcc accgccactg aaaccaccgc ccgccccgcc
     1321 actactgcgg cggtcttcca cgttgtctga ttcgcggttg ccttcccatt tcatggtgtg
     1381 gctcctaaaa ggtttatgcc ttgggcaagg tcacgccatg ctggccctgg tatttgccgc
     1441 cgcggtcctt gtaggacact tcgcagacgt cgtccttgtc gctctcgaag aacagcacct
     1501 gggcgcagcc ttcaccggcg taaatcttgg cgggcagcgg cgtggtgttg gagaactcca
     1561 gcgtcacata gccttcccac tc
//
LOCUS       DAOSJM010000435         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_68670_length_1571_cov_2.995383, whole genome shotgun sequence.
ACCESSION   DAOSJM010000435 DAOSJM010000000
VERSION     DAOSJM010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68670_length_1571_cov_2.995383"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PKX82_12320"
     CDS             complement(<1..874)
                     /locus_tag="PKX82_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HOF53085.1"
                     /translation="MLDPRTVSCAYVRTLLTTVEAQGVPSAQVLHGLPVEPGQIAQAN
                     GRLALGVVRLIWDRALALTADPSLGLKVAQDMKPNTFRVLGMATMSCASLAQAIGVML
                     RYQRLVSESGTLAAHPQPDGSVALEHTVQTARVPILPQQVEALLGGVYRQALWLAGRA
                     YQPLEVSFRHPAQGPLQGYRQCFGITPQFAADANRLTFSARDLSAPLPHADADLCRLH
                     CDMADRELASLPQVGYASSFAVQWLAHRADANPSIEDLAAALGLSVRSMQRTLKEEGT
                     SWATLVDNMRRAALE"
     gene            992..>1289
                     /locus_tag="PKX82_12325"
     CDS             992..>1289
                     /locus_tag="PKX82_12325"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOF53086.1"
                     /translation="MQAHRLENEVHIQRPPETVFRYVTQPWLWHEWHPASESAVGSAN
                     PLKVGDHFDESVSMQPFGWFPIRIRRQLRWTVVESQEPHLWEVHGTSPTIDVRAR"
     assembly_gap    1290..1299
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          295 a          527 c          497 g          242 t
ORIGIN      
        1 cttccagcgc cgcacgccgc atgttgtcga ccagcgtggc ccaactcgtc ccctcctcct
       61 tcagggtgcg ctgcatgctg cgcacgctca gtcccaatgc tgccgccaga tcctcgatag
      121 acggattggc gtcggcgcgg tgtgccagcc actgcaccgc aaagctggag gcgtacccca
      181 cctgcggcag gctggcgagc tcgcggtctg ccatgtcgca atgcaggcgg cacaggtcgg
      241 catcggcgtg cggcaagggg gcgctcaggt cccgtgccga aaaggtgagg cggttcgcat
      301 cggctgcaaa ctgcggcgtg atgccgaaac actgccggta gccttgcaac ggcccctgcg
      361 cgggatggcg aaaactcact tccagcggct ggtatgcgcg ccccgccagc cacagtgcct
      421 ggcggtacac accaccgagc aatgcctcca cctgctgcgg caggatgggg acgcgagcag
      481 tctgtacggt gtgctccaaa gccaccgaac cgtcgggctg agggtgggcc gccagggtgc
      541 ccgactcgct gaccaggcgc tggtaacgca gcatcacgcc aatggcctgc gccagcgacg
      601 cacagctcat ggtggccatg cccagcacgc gaaaggtatt gggcttcatg tcttgcgcca
      661 ccttcaagcc cagcgacggg tcggcggtga gcgccagcgc ccggtcccag atcaggcgca
      721 ccacacccaa agccagccgc ccgttggcct gtgcaatctg ccccggctcc acgggaaggc
      781 cgtgcaagac ctgcgccgat ggcaccccct gcgcctcgac ggtggtcagc agcgtgcgga
      841 cataggcgca ggagacggtg cggggatcga gcatgcgcga ccataggcag attggcgcgg
      901 aatgtcaatg caacgacagg gtgcagtcaa tgccagccgc agccgaatcc acaacaatgc
      961 cagggcacca caaaaaacaa caggagacac gatgcaggca caccgacttg agaacgaggt
     1021 tcacatccag cgaccccctg agaccgtgtt ccgctacgtg acgcagccgt ggctctggca
     1081 cgaatggcac ccggcctctg agtccgccgt aggcagcgcc aacccgctga aggtgggcga
     1141 ccactttgat gagtcggtca gcatgcagcc gttcggatgg tttcccatcc gcatacggcg
     1201 ccagctgcgc tggaccgtgg tggagtcgca ggaaccccac ctctgggagg tgcacggcac
     1261 atcgcccacc atcgacgtgc gcgcccgctn nnnnnnnnnc gctatgagct cgacccggtg
     1321 gatggcacgc ggttccggcg cacctttcac tatcgggtca aaggtccgct tcgggtgatt
     1381 gatcgcggct ttgtcttgcc gcgcatgcaa cagcaatccg tggtggcgct gcagaacctc
     1441 caacgcgcgc tggaggccca atgaaccacg ccgttgcaga gttccgcgcg cagtaccgcg
     1501 agcagcatat tggtcgaagc taccggggct ggctgcactt cggcttcacg gtcagcatgg
     1561 caagcaccat c
//
LOCUS       DAOSJM010000436         1570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1675
            NODE_68716_length_1570_cov_4.525413, whole genome shotgun sequence.
ACCESSION   DAOSJM010000436 DAOSJM010000000
VERSION     DAOSJM010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863204
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 01:04:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,468
            CDSs (total)                      :: 2,456
            Genes (coding)                    :: 2,437
            CDSs (with protein)               :: 2,437
            Genes (RNA)                       :: 12
            tRNAs                             :: 10
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 8 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 2 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68716_length_1570_cov_4.525413"
                     /isolate="AMR_MDS_1675"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="PKX82_12330"
     CDS             complement(<1..766)
                     /locus_tag="PKX82_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HOF53087.1"
                     /translation="MNKPAIPQAPARFQPFDLLATLVAVVHGDGSVIFSNAALEDALG
                     TSRRMIVGTSFTEVFTEPTQLQKALEGASENEFAALRYDAWLKRIGQEPMPVHVIITR
                     PEHTDEIIVEMVPLEQQARQDREERLAEQAQTNKELIRNLAHEIKNPLGGIRGAAQLL
                     EMEIESPELTEYTQVIIHEADRLQSLVDRLLAPHRRPHLVGDVNIHEVCERVRSLILA
                     EFPKGLQVVRDYDISLPEFRGDREQLIQTVLNIAHNA"
     gene            complement(772..1263)
                     /locus_tag="PKX82_12335"
     CDS             complement(772..1263)
                     /locus_tag="PKX82_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF53088.1"
                     /translation="MKTVFFFSLIAMGCATGALAQDRIYRCGNEYTNTVSEAQAKNCK
                     LVSGGNVTVVQAQKPGAGNKAASGTPAQRVDAGDQRAKDSDARLILESELKKAEARQA
                     ELLKEYNNGEPEKLGPETRNHQKYLDRIAELKASLARNESDIAGIRRELGRVPSSAQS
                     GAK"
     gene            complement(1440..>1570)
                     /locus_tag="PKX82_12340"
     CDS             complement(1440..>1570)
                     /locus_tag="PKX82_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOF53089.1"
                     /translation="FLTKGGVFTDSMIDAYIELKMQEVTRYRMSVHPVEFDMYYSL"
BASE COUNT          307 a          472 c          439 g          352 t
ORIGIN      
        1 aggcgttgtg cgcgatgttg agaactgtct ggatcaactg ctcgcgatcg ccacggaact
       61 ccggcagcga tatgtcgtag tcgcgtacca cctgcagccc cttgggaaac tcagccagaa
      121 tcagggagcg cacccgctca cagacttcgt ggatattcac gtcaccgacc agatgcggtc
      181 ggcggtgcgg cgccagcagg cgatcgacca ggctttgcag gcggtccgcc tcgtggatga
      241 tgacctgggt gtactccgtc agctcgggcg actcgatttc catttccagc aactgggcag
      301 ccccacggat accacccagc ggattcttga tttcgtgcgc cagattacgg atcaactcct
      361 tgttggtctg cgcctgctcg gccagacgct cttcacggtc ctggcgcgcc tgctgttcca
      421 ggggaaccat ctccacaatg atttcgtcgg tgtgctcggg gcgcgtgatg atcacatgca
      481 ccggcatggg ctcctgtccg atgcgcttga gccatgcgtc gtaccgcagg gctgcaaatt
      541 cgttttcact ggcgccttcc agcgccttct gcaactgcgt gggctccgtg aatacctcgg
      601 taaatgaggt ccccacgatc atgcggcggg aggtgcccag cgcatcttcc agtgcagcat
      661 tggaaaaaat gacggaaccg tccccatgta ccacggccac cagggtggcc agtaggtcaa
      721 agggctggaa gcgcgctggc gcttggggaa tagcaggctt gttcaagctg cctattttgc
      781 cccagactgc gcactggagg gcacgcgtcc aagctctctg cggatgcctg caatatcact
      841 ctcattacgg gcaagactgg ccttgagttc agcgatccgg tccaggtact tctggtggtt
      901 gcgtgtctct ggccccagct tttcgggttc gccgttgttg tattccttga gcagttcggc
      961 ctgacgcgcc tctgctttct tcaactcgga ctccagaatc aggcgcgcat cggaatcttt
     1021 ggcacgttga tcacctgcat ccacacgctg ggctggcgta cccgacgcag ccttgttacc
     1081 ggctccaggc ttttgtgcct gcaccacagt cacatttcca ccggagacga gcttgcagtt
     1141 cttcgcctgg gcttccgata cggtgttggt gtattcattt ccgcagcggt aaatacggtc
     1201 ctgtgccagt gcgccagtcg cacagcccat tgcaatcaag gaaaaaaaga aaacagtttt
     1261 catgcgatat ccgccttgca catgcagggc catgcgggaa gtatgcgcca aaaaccggcg
     1321 attccccata tcccctatgg acttaacgcc catcactaca aagggttaca tccggggcaa
     1381 aaaaaaggac ggcttgcgcc gtccttctct agtacatctg ccaccagggc agtgccccat
     1441 tacagggagt agtacatgtc gaattcgaca gggtgcaccg acatgcggta acgggtcact
     1501 tcctgcatct tcagttcgat gtaggcatcg atcatgctgt cggtgaacac gccacccttg
     1561 gtcaggaacg
//
